LOCUS       DASRLV010000001         8227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1056605,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000001 DASRLV010000000
VERSION     DASRLV010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 8227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8227
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056605"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VFC43_00005"
     CDS             complement(<1..259)
                     /locus_tag="VFC43_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29607.1"
                     /translation="MEGRDVRQSLGKLLGGKGTNQPATPPPRNETAHEEVIEIIDEDP
                     KSAVRNKAIKNAVLTPPAAVQVTDADQKIVNVDQISEISGLI"
     gene            complement(395..625)
                     /locus_tag="VFC43_00010"
     CDS             complement(395..625)
                     /locus_tag="VFC43_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29608.1"
                     /translation="MSGLNNDDDEALDNVDSLINRIKGKKSEEPQVQKNADSPLKASP
                     AGGSSAPGIGDFSALRKKMETAKTEEPSPPLR"
     gene            complement(773..1501)
                     /locus_tag="VFC43_00015"
     CDS             complement(773..1501)
                     /locus_tag="VFC43_00015"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HZK29609.1"
                     /translation="MRLPAGTVERIITNPKKEGLFQYLMTFKGLIETETPSGHGFILI
                     DNGIPVSAYFKNADALFRGHSALAPMNMESDADFESPQTLNVRKYTDEEYSRAVQISL
                     EEDLIIHKPGTPSGGDNEDLFTDEPAEPPEPLDETKLKKIKGLAGVIAVAAFSEGFAI
                     QSLGDADFEHVAASAEDLMRAGTKIARDLKIGALDQMILETDENKFIIAPCGDLYICV
                     FTTADAHLGLIRVVLKNIQSEISG"
     gene            complement(1519..1866)
                     /locus_tag="VFC43_00020"
     CDS             complement(1519..1866)
                     /locus_tag="VFC43_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HZK29610.1"
                     /translation="MLKPLLEEFLKIEGVSAAVVIGRDGFVIESAVSGKVDIEALGAM
                     ASTGLGTSEAMGSTLGKGDLSQMLVELEKGPIILSPLSADELIAIVADTTANIGRIRY
                     ELKKNKERLIAAL"
     gene            2113..2892
                     /locus_tag="VFC43_00025"
     CDS             2113..2892
                     /locus_tag="VFC43_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29611.1"
                     /translation="MQLPRGTFLTIKRSTKFGDLFSELHEMKFTGVCTISAKSGNGTI
                     VFKHGKRILAEFQNTTGDTAWDDVQKIISEKGDASLSTLSEAQIKLSLEFNKTCLVAK
                     GAKTEKPALMNIPVPPPISTTPTEITPPQQAKAVPVKTHQKPGIPQFAPLPEPGTVQQ
                     VKSPQPPRIIRDSQLRTATQLAEGQVKTAVPPQNEGNKFPDLSRDQEESESSSFEADI
                     ETFETMDVEAIRNKIHGECKSLIKELNLEHLTESHTDSKKR"
     gene            2899..3486
                     /locus_tag="VFC43_00030"
     CDS             2899..3486
                     /locus_tag="VFC43_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29612.1"
                     /translation="MISLQTWPDSPQSIVVIGLVALATMGAAALIISMLQKKIRILKS
                     RRKFATFPPQYQNFHHETKLIRKMVPVVQSQRAHKTPALRIEEVELLSGREDITQSLM
                     ALVEKYSLITFTIATSDGLVFASSGGDDANINAATYTTMALRDPLTKTTMVIIPGLIH
                     KGSDLVGIIRTQNPLEEEILMNITKDTQTILNWWV"
     gene            3554..4348
                     /gene="minD"
                     /locus_tag="VFC43_00035"
     CDS             3554..4348
                     /gene="minD"
                     /locus_tag="VFC43_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATPase MinD"
                     /protein_id="HZK29613.1"
                     /translation="MVKVYTIASGKGGTGKTTVSVNLGTMLAQLGKETFLMDADIGMA
                     NVGLLLGLQDAPVTLHEILAGKGKIDDAIYECPAGLKVIPAGISLQGFQQADPEKIRD
                     VMNEIVKRCDFLLIDAPAGISKDGIVPLAVADEVILVVNPELSSIVDALKTRILTELV
                     GGHVLGSIINRVNQDNPETMIKKMEKVLGVKVIGIIPEDPNVRRAGSARIPLVIKFPT
                     SPASKAFRRIASGLIGVAYKDEGEDEKREGFIERFTKALFTKKSKK"
     gene            complement(4590..5699)
                     /locus_tag="VFC43_00040"
     CDS             complement(4590..5699)
                     /locus_tag="VFC43_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V4R domain-containing protein"
                     /protein_id="HZK29614.1"
                     /translation="MKIGREDFEALIKGEKTIAPYIEMDPLAGSYSVFGVKTASNPNY
                     LVKAIYEMHEANLDKKLAVQAVRKLFNAVGRGSVGLNNLLKKYGMALTPSEFLLLVFK
                     LQHQQGWGAPFEIIESSDTRIVLRTGHTFESEVLKDWKMPVCGIHQGWIEGVLIAVTG
                     KNWLCTETKCHAKEDDCCEFIAEQRDPGWKGKAEAIVKGESAITEYIEHKPLEGKIRL
                     IDDPVVIMPRFIFTSMMNSLKKTMGEMPAGGVNYRAYMDMGRENVGHYKNMGITSPTL
                     LSDMALAFYSQMGWFRILKMEWNEQKKEKTITLSHTSESDAFGNTGKNVCYCTAGLLA
                     GIVEGAFGIKVQGREIRCRSKGDEHCVFTITNKPE"
     gene            complement(5813..6787)
                     /locus_tag="VFC43_00045"
     CDS             complement(5813..6787)
                     /locus_tag="VFC43_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29615.1"
                     /translation="MKIQDMIKNIHKGSKHLGTFTLHELFQFASSRNFNGIAVAKDNG
                     REHYLAFLAGEAEGAIYIDDNGTEYSDKAAKMITGNEKFEFYEITPDIVETIVMRCRI
                     FEKTHLKKTVPNVIHDLLDNIIKGSKYVGVFPMHELFSFASTGRSNGIAASKENEREK
                     YLAFIDGEAEGAIYIDDKGSLYSDKAVLMISGQEKFEFYNVKPDIVDAVVMGSRIFEK
                     MHLKKSAPSLIPEIGKKSEGIGVLTVTITKDTVPLNGVSISIRRDGKILGSDITTGQG
                     TAGFKLKYGKYDCIVKDRSQNMTTYYITFDESKSKILLDLTAKHLLDI"
     gene            6977..8149
                     /locus_tag="VFC43_00050"
     CDS             6977..8149
                     /locus_tag="VFC43_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HZK29616.1"
                     /translation="MIISSVVVLATLIYASFLDIRDRRVPFVHWLPMLGVGSICTSVL
                     LWQTTGNLSIITGYLALVASFLYADYLDNRGRTDSVGLIVYYQKASIFYYLAIVLVLP
                     ALSWFVFMPSVNFQLVPWYAMFAGVISYISYMEYRGMFDEPVRKKGKKQGQAFNEIIS
                     RWYFVLVILIFAITSVLMLFGPGGWGAPALVLLLLSVFCGVFYVFGRMNLFGGADAWA
                     LIFVAFCIPTFPFTPLLGDPPLGFLAFSALINALILNMVAPVAIFVMNVIRKNRAPLM
                     YMFFGFPVNGDNIQHEWGFVMEDFSEKNGKVSRTFIGFFDSIRRMYSGEGRIYTKDLR
                     EHPEKFKKELAMYRNAGTVWISYAVPFIIPITAGLITAIVFGDILFAIMKMVAGGI"
BASE COUNT         2143 a         1910 c         1845 g         2329 t
ORIGIN      
        1 ggataagtcc ggagatctct gagatctggt caacattgac aatcttctga tctgcgtccg
       61 tgacctgcac agcagcaggg ggggtcaaaa ctgcattttt tatcgcttta ttccgaacag
      121 ctgacttggg atcttcatca attatctcaa taacctcttc gtgtgctgtt tcattgcggg
      181 gtggcggagt ggcgggttga tttgttccct taccccccag aagtttacca agggactgcc
      241 gtacatccct tccttccatc agagtatcag gttcggcagg gggcttttgt gatatctggg
      301 cggagtgcgg gaatttttcc tgcagatcgt ctgcatcgga aaattccaga tcatcatgcg
      361 gggatgctgg cggagtgcgg ggcgtgactg acggctaccg cagcggggga gagggctcct
      421 ctgtttttgc tgtttccatt ttttttctga gcgcggagaa atcaccaatg ccgggtgcgg
      481 agctgcctcc cgccggtgat gctttaagag gagaatcagc atttttctga acctgtggct
      541 cctcactttt tttgccttta atcctgttga tcaaagaatc tacattatcc agtgcttcat
      601 catcatcatt gttcaatcca gacatcaccc tttgaactag atttaggtct tcatttatat
      661 taaatgattt ttttttacat tttaaagtat atatagtata gctattgact tagtgaacat
      721 tctggtcgat ttttaataaa aatgtcatct tcaatgttgg aaaatccgtt tatcacccgg
      781 aaatctctga ctggatattt tttaatacaa cccggatcag accgagatgt gcatccgccg
      841 tggtaaacac gcagatgtaa aggtccccgc atggggcaat aatgaattta ttttcatccg
      901 tttcgagaat catctggtca agggcaccga ttttcaggtc ccgggcaatt ttggtccctg
      961 cccgcatcag gtcttcagct gaggctgcca cgtgctcaaa atcggcgtcc ccaagagatt
     1021 gaatggcaaa tccttcggaa aatgcggcaa ccgcaataac gcccgccagg cctttgattt
     1081 tttttaattt tgtttcatcg agtggttcag ggggttctgc tggctcatcg gtaaacaggt
     1141 cttcattatc cccacctgag ggagtaccgg gtttatggat aataagatct tcttcaaggg
     1201 aaatctgaac tgcacgggag tattcttcat cagtgtattt tcgtacatta agtgtctgcg
     1261 gactttcaaa atcagcatcc gattccatgt tcatgggagc aagggctgag tgtccgcgaa
     1321 ataatgcatc ggcatttttg aaatatgctg aaaccggtat cccgttatca ataagaataa
     1381 aaccgtgacc ggagggtgtt tcggtctcaa tcagtccttt gaatgtcatc aggtactgga
     1441 acaacccttc ttttttggga ttggtaatga tcctttccac ggtgcctgcg ggtaatctca
     1501 taaaaaaagg acttggtatc agagagcagc aataagccgc tctttatttt tctttaattc
     1561 gtacctgatt ctgccgatat tggcggtggt atctgcaaca attgcgatga gttcatcagc
     1621 tgaaagcggt gacaaaataa tcggaccttt ttccagttct acaagcatct gggagagatc
     1681 tccctttccg agcgttgagc ccattgcttc tgatgttcca aggccggttg atgccatggc
     1741 tcccagcgct tcaatatcaa ctttgccgga aacagcactt tcaatgacaa agccatctct
     1801 tcctataaca accgcagctg ataccccttc aatttttaag aattcctcca ataatggctt
     1861 cagcattcac gtaccctcag ataaaagatt ctctcttgtt ttttttctat taaaatgttg
     1921 gtcttttggt gcccggtaag aataatgcag gtaaaatttc aaaaaaattt gaaaagaacg
     1981 ttttttcttc ggtcttttaa gagcgttgcg tgagcgtcag aacaggaggt tttaccgcat
     2041 tcaacaaaaa catccccgga agttatcgta aaaaaggaaa ctatatagta gaatatcata
     2101 acatacaaca taatgcaact cccgaggggt actttcctta ctattaaaag gagtacgaag
     2161 tttggggacc ttttttcaga acttcatgaa atgaaattca ccggagtatg cacaatctcg
     2221 gccaagtctg gcaacgggac gatcgtcttt aagcacggta agcgaatcct tgcagagttc
     2281 cagaacacca caggtgatac tgcctgggat gacgtccaga aaatcatctc ggagaagggg
     2341 gatgcttccc tctcgacact gagtgaggct caaatcaagc tctcgctgga attcaacaaa
     2401 acctgtcttg tggccaaagg agcaaaaaca gaaaaaccgg cccttatgaa catcccggta
     2461 ccacccccga taagcacgac tcccactgag attactcctc cacaacaggc aaaggccgta
     2521 cccgttaaaa cccaccagaa accgggtatt cctcaatttg ctccattacc tgaacctggc
     2581 acggttcaac aggtaaaaag tccacagccc cccaggatta tacgtgacag ccagctccgg
     2641 acagcgactc aactggcaga aggccaggta aaaacagcgg tacctccaca aaacgagggg
     2701 aataaattcc cggatttgtc acgtgatcag gaagaatcag aatccagcag ctttgaagca
     2761 gatatcgaaa cctttgaaac aatggatgtg gaagcgatca ggaataaaat ccacggtgaa
     2821 tgcaaatcac tgatcaaaga actcaatctt gaacatctga cagagagcca tacggatagc
     2881 aaaaaaaggt agatactggt gattagtctt cagacgtggc cagacagccc gcagagcatt
     2941 gtcgtcattg gtcttgtggc tctcgcgaca atgggtgccg cagcgctgat tatttcaatg
     3001 ctccaaaaaa aaatccgtat tcttaaatca agacgaaaat tcgccacctt cccaccacaa
     3061 taccagaatt ttcaccacga aaccaaactt atacgaaaga tggtaccggt agtacaatcc
     3121 cagagagcac ataaaacccc ggctcttaga attgaggaag tcgaactcct gagcggccgg
     3181 gaagatatta cccagagtct tatggcgctt gttgaaaaat attccctcat cacgttcacc
     3241 attgccactt cagatgggct tgtatttgct tcgtccggcg gggatgatgc gaatatcaat
     3301 gcggcaacct atactacaat ggcattaagg gatccgctta caaaaactac aatggttata
     3361 atacccggtc ttatccacaa agggtccgac cttgttggaa taatacggac gcaaaatccg
     3421 ttagaggaag agattttaat gaatattacc aaagatacac aaacaatatt aaactggtgg
     3481 gtttagttca ataattaaaa attgggtaat cttatataaa tcaaatagaa atattcacat
     3541 gacagggtgt ttaatggtca aggtttacac aattgcttcc ggaaaaggtg ggactgggaa
     3601 gacgaccgtt tctgttaatc tgggaacaat gctcgcccaa ctcgggaaag aaaccttcct
     3661 gatggatgct gatatcggta tggcaaatgt cggcctgctt ctcggtctgc aggatgcacc
     3721 agtcaccctt catgaaattc tagccggtaa agggaagata gacgatgcga tttacgaatg
     3781 cccggcggga ctcaaggtca tccccgcggg catatcactc cagggattcc agcaggcaga
     3841 tccggaaaaa atccgtgacg tcatgaatga gattgtcaaa cgttgcgact ttttattaat
     3901 cgacgcccct gcagggatca gcaaggacgg gattgttcct ctcgcagtcg cagatgaagt
     3961 tatccttgtc gtaaacccgg aactttcttc gatagtcgac gccctcaaga cacggatact
     4021 gactgaactt gtcgggggtc acgtgcttgg ttctatcatc aatcgtgtca atcaggacaa
     4081 tcccgaaacc atgattaaaa agatggagaa agtgctcggg gtcaaggtta tcgggattat
     4141 tccagaagac ccgaacgtcc gtcgtgcagg ttctgccagg atacctctcg tgatcaagtt
     4201 cccgacatcc cctgcctcaa aagctttccg ccgtattgca tcaggtctta tcggtgtggc
     4261 atataaagat gagggggaag acgagaagcg cgaaggattc atcgagcgat tcacaaaagc
     4321 cctgttcacc aagaaatcaa aaaagtaaag ccagtatttt ccaggagagc acaaaatact
     4381 ttttttcctg tagccattaa atcagcatgt atttgagaga tccatccctt tttctcccat
     4441 tggcttgcgt ccacaactca aatcacggtt aagcattggt atctcagctg actatccgtc
     4501 caggtaaaaa aacaggattt tacagcagta aattcagttt ctttcaagag tttttttaaa
     4561 actaccaagg gtgagaaggg aaaaatgatt tattccggtt tattggttat cgtaaaaaca
     4621 cagtgctcgt cgccctttga acggcacctg atttcccgac cctgcacttt tattccaaag
     4681 gcaccttcaa caatgccggc aagaagacct gcggtacaat aacagacatt ctttccggta
     4741 ttcccgaatg catcggattc tgaagtgtgt gacagcgtga tggttttttc cttcttttgc
     4801 tcattccact ccattttgag gatccggaac cagcccatct gggaataaaa ggccaatgcc
     4861 atatcggaca ggagggttgg gctggtaatc cccatgttct tgtagtgtcc gacattttcc
     4921 ctgcccatat ccatataagc ccggtaattt actccgccgg cgggcatttc gcccatggtc
     4981 ttttttaagg aattcatcat ggacgtgaat ataaaacgcg gcattataac aaccggatca
     5041 tctatcagac ggatctttcc ttcaaggggt ttatgttcga tatattcagt gattgctgat
     5101 tcacctttga ctattgcctc ggctttccct ttccagcccg ggtcccgttg ttcagcgata
     5161 aattcacagc aatcatcctc ttttgcatgg cactttgtct ctgtgcagag ccagttcttt
     5221 cccgttacgg caatcagaac accctcaatc cagccctggt gaataccaca gaccggcatc
     5281 ttccagtcct taagaacttc ggattcgaac gtgtggccgg tacggaggac gatccgggtg
     5341 tcgctggatt caataatttc gaaaggagca ccccagccct gctggtgctg gagtttaaaa
     5401 accagcagga gaaattcaga aggtgtgaga gccattccgt atttttttaa gagattattc
     5461 aggcccaccg atcccctgcc aaccgcgttg aagagcttgc gaaccgcctg gacagcaagt
     5521 tttttgtcga gattcgcttc gtgcatttcg tagatggctt tcacgagata attgggattg
     5581 ctcgctgttt ttaccccaaa aactgaatac gaaccggcca gagggtccat ctcaatatac
     5641 ggggcaatgg ttttttcccc ttttatgagc gcctcgaagt cttcacgtcc gatcttcatg
     5701 gaggagaact ctgttattat tcaattaaag gatttcgtcc attttacatc aggcgtacac
     5761 atattcccga aaaatgatga aataacaagt aaaaacgggc aatacacaac tgttaaatgt
     5821 ccaggagatg ttttgcggtc aggtcaagaa gaatttttga ttttgattcg tcgaatgtga
     5881 tgtaatacgt ggtcatgttc tgggaacgat ccttgacaat acaatcgtat tttccatact
     5941 tcagtttgaa accggctgtt ccctgtccgg tggttatgtc gcttcccagg atcttcccgt
     6001 cacgccggat cgagatgctg acgccgttta agggtaccgt gtctttggtg atggtgaccg
     6061 ttaacacccc gattccctca cttttctttc cgatctcggg tattaaggac ggggcgctct
     6121 tctttaaatg catcttttca aaaatccgtg atcccataac aacggcatcg acaatatccg
     6181 gctttacatt atagaattca aatttttcct ggcctgaaat catcagcacg gctttgtcgc
     6241 tgtagagaga ccctttgtca tcgatataga tggcaccttc agcttctccg tcaatgaatg
     6301 caagatattt ttcgcgttcg ttttcttttg acgctgcaat accattggat cgtcctgtcg
     6361 aggcaaacga gaagagttca tgcatgggaa ataccccgac gtactttgaa cccttgataa
     6421 tattatccag caggtcgtgt atgacattcg ggacagtctt ttttaagtgt gtcttttcaa
     6481 aaatccgaca cctcataacg atagtttcaa caatgtccgg ggttatttcg tagaattcga
     6541 atttttcatt gccggtgatc atttttgctg ctttatcgct gtattctgtg ccattgtcat
     6601 cgatatagat ggcaccttca gcctcaccgg caaggaaggc aagataatgt tccctcccat
     6661 tgtcttttgc caccgcaata ccattgaaat tccgtgatga ggcaaactgg aacagttcat
     6721 gcagggtaaa ggttcccaga tgctttgagc ccttgtgaat attttttatc atgtcctgta
     6781 ttttcataaa aaactcatct cggcgggaac tgcttatggg aattcattgc acaattttcc
     6841 ttatgtatag tggagatatt tgcaatgtgt gtattttaca tttgcggaag gattttttcg
     6901 atcttttttc ctatcgccat acataagtgt tcgtaaaaaa aaccggtata ccggatttat
     6961 ggaaatactc taccctatga taatttcatc agttgtggta cttgccaccc tgatctatgc
     7021 atcatttctt gatatacgtg accgccgggt cccgtttgtc cactggttgc ccatgctcgg
     7081 tgtggggagt atctgcacca gtgtcttact gtggcagacc acgggtaacc tgagcatcat
     7141 caccggttac cttgccctgg tcgcaagttt tctctatgct gattacctgg ataacagggg
     7201 caggaccgat tctgtgggcc ttatcgttta ttaccagaag gcttcaattt tttattacct
     7261 tgcaatagtc ctcgttcttc cggccttgtc atggtttgtt tttatgcctt ctgtaaattt
     7321 ccagctggtc ccctggtacg ccatgtttgc cggagtaata tcctatattt cctacatgga
     7381 ataccgtgga atgttcgatg aacctgtccg gaaaaaaggc aaaaaacagg gccaggcctt
     7441 caacgagatc atcagccgct ggtattttgt cttggttatc ctgatttttg caattacatc
     7501 ggtactcatg ctctttggcc cgggtggctg gggagccccg gcccttgtgc tccttctcct
     7561 gtcggttttt tgcggggttt tttacgtgtt cggaaggatg aacctctttg ggggtgccga
     7621 tgcgtgggcc ctgatttttg ttgcattctg tatccccaca ttcccgttta ctcctctcct
     7681 tggcgacccg ccgctgggat ttttggcatt ctctgccctc atcaacgcac ttatcctcaa
     7741 tatggtggca ccggtcgcga tttttgtcat gaatgtaatc cggaaaaacc gggcgccact
     7801 gatgtacatg ttcttcgggt tcccggtaaa cggcgacaac atccagcacg agtgggggtt
     7861 tgtcatggag gatttttccg aaaagaacgg aaaagtcagt cggactttca tcggattttt
     7921 cgactccatc aggcggatgt attccggtga aggcaggata tacaccaaag acttgcggga
     7981 acatcccgaa aaattcaaaa aggaactggc gatgtacaga aatgcaggaa cagtatggat
     8041 ttcttacgca gtccccttca tcatccctat tactgcaggt ctgattaccg caattgtttt
     8101 tggcgatatc ctgtttgcga ttatgaaaat ggtagcggga gggatatgat atgctcacgg
     8161 taaaatttgt agacggcctg gtcccggtaa ttgtccagga ttcaaagagc cgcgaagtac
     8221 tgatgat
//
LOCUS       DASRLV010000002        14193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_975351,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000002 DASRLV010000000
VERSION     DASRLV010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 14193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14193
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975351"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="VFC43_00055"
     CDS             <1..779
                     /locus_tag="VFC43_00055"
                     /inference="COORDINATES: protein motif:HMM:NF024270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3821 domain-containing protein"
                     /protein_id="HZK29617.1"
                     /translation="IGRTTSFTVSQSEFDGYLGNWYRLNSAGKSDGSAFNVADPQLEI
                     KAEDTTVAVDPTITWIPSGDDIRFKIDTNLVQMASQRASPPLITIKVEGPDGGSYTSL
                     YNAGGSPSSIVDIPVTTRPFYTASIWNMGNRDRYPPGTYTYWAECNVNRMNDNYDVTG
                     KTVSRKITLLNQGVNPLITKPTTVQTTKTLQPATTQLTTIQTTPADTGTIVSTPSLSV
                     SPTTTAPAVPTAGQTKAPGFGVTLAIFAIFFGLAAYFKNH"
     gene            837..1343
                     /locus_tag="VFC43_00060"
     CDS             837..1343
                     /locus_tag="VFC43_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZK29618.1"
                     /translation="MNIVTTVIKSRHSVRKFKPDPVDDKVIRDAIECAAHAPTAKNIQ
                     PWLFGVIKNKDMLKAIAQSTDNGKFIADAPMCVAVFGERNEKYYLEDCCAATENLILA
                     LQAYGINSCWVAGEKKPYAESIRTLLGVPEKYTLVSLVAAGCPRELTIATKKEQKQIV
                     FYEEFKKE"
     gene            1486..2772
                     /locus_tag="VFC43_00065"
     CDS             1486..2772
                     /locus_tag="VFC43_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hexulose-6-phosphate
                     synthase/ribonuclease regulator"
                     /protein_id="HZK29619.1"
                     /translation="MKKAVLQVALDIIELKRALQIAQESIDGGADWIEAGTPLIKSEG
                     MQAIRSMRDRFPGSIIVGDMKVADTGTLEVEMAAKAGANIVCILADADDSVIAESVRA
                     ARLYGIRIMADLINVRDPIKRAPELEAMGVDIICAHVGIDQQMIGKNSIDLLTSLSGL
                     IHIPLAVAGGISAATAGDAVKCGADIVIVGGWIVRSAEVSQNTRKIRAEMDDPLVRTI
                     EKKSPDEEIRSLLLQVSAPNITDAMHRKGAMSGILSICGNVKMVGKAVTVQTVAGDWA
                     KPVEAIDVAGKNEVIVINNDGAVHIAPWGELATLSCMKKGISGVVIDGAVRDVDDIRA
                     MKYPVFAKAIVPNAGEPKGFGEINAEIQCCGQSVCPGDWIVGDESGVVVIPSVRAYEI
                     ARRALEVRKNEERIREEIHRGSTLSQVAELIKWEKK"
     gene            complement(2779..3213)
                     /locus_tag="VFC43_00070"
     CDS             complement(2779..3213)
                     /locus_tag="VFC43_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HZK29620.1"
                     /translation="MDTQKYVIRQISIFSENRPGRLAAIAHALGEENINILAFSIAEA
                     NGFGVVRALVDHPEKAHDKLFSLGFNVAFTDVIAVRMKDQPGGLYEVAKVLGDAGVNI
                     EYSYAYSGKEGAVLILRVDMVDEAIKKIKNSGATLLERSVFH"
     gene            complement(3213..4514)
                     /locus_tag="VFC43_00075"
     CDS             complement(3213..4514)
                     /locus_tag="VFC43_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HZK29621.1"
                     /translation="MKSWDPRIEEMPREDLRRMQYKLLKSLVYRLYSFSPFYHDRMKE
                     QHVHPDDIRELDDVKKLPFMFKRDLRDNYPDKIFTSSQEELVRYHVSSGTTGKPTVVG
                     YTQNDLNLWTTSLARALTSIGLGRGDVIQVSYGYGLFTGGLGMHYGAERIGATVLPTS
                     VGNTERQIELMQDLGATAIACTPSYLLHIGEVAEKMGVDIKKDTQLRTGVLGAEPWTD
                     RMRTRIEDWLGIKAYDIYGTSELSGPMFTECVEQKGFHIWSDIALVEIIDPKTGEPLE
                     PGQKGELTITILQKEALPMIRYRIGDITTMDDEVCACGRTHPRVQRIQGRVDDMLIIR
                     GINVFPSQIEYALMAIPEVGQHFQIVVERKGALDDMLVRVELNKESFSDKINDLMQVR
                     EKVEHRLGNSLNVNVNVELVEPGSLPRFEGKAKRVVDKRSL"
     gene            complement(4514..5803)
                     /locus_tag="VFC43_00080"
     CDS             complement(4514..5803)
                     /locus_tag="VFC43_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HZK29622.1"
                     /translation="MFWNKKLETMKPEELKTLQLKRLKKTLSQVQNVEFYRNLLAGSG
                     LKTSSIKTLDDIQKIPFTKKQDLRNGYPFGFFAVPMKKIVRIHTTSGTTGKPTVVGYT
                     RRDLDTWSDLIARNMTMVGVRKNDIFQNMVNYGMFTGGLGFHYGAEKIGMTVIPSATG
                     NTKRQIEMILDFGVTTIHCTPSYAMHLSEIAEEMNASLDSLRTGLFGAEPWSESVRHS
                     LEKRLGLTAYDSYGLSELFGPGVAFECPERNGLHIWHDSFFVEIIDPKTGEQLSDGER
                     GELVVTPLVKEAMPLLRYRTGDVTVLMEDGCLCRRSQKIARITGRSDDMLVIRGINVF
                     PSQIEHVLLGIPEVGNQFMVYIDRINHLDEMTVEVEINREYFSGELADLAKLQKKVVK
                     ELRDALELRTTVKLVEPGSLPRFEGKAKRVIDRREAM"
     gene            6035..6946
                     /gene="cas1"
                     /locus_tag="VFC43_00085"
     CDS             6035..6946
                     /gene="cas1"
                     /locus_tag="VFC43_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HZK29623.1"
                     /translation="MPVSGFGAHIKSTQKKLIIQKKNTLEEYPLDSIKNLLVIGGHTI
                     SSSTIVSLVKNGAFISFFEPDGNPVGIIRPFGDQNDRDVYTWQQGVPRHRYAIILAQA
                     AIKSRLFAIQRVQEIRNTHLFYKGEQEFLHASQDELQYLIKLDEIRRLHRLTSDMYYE
                     IMSRNISSDFGFGRRTIRPQADPVNAMLSFGYAMLYGNCCVSVIGARLNPDVGLMHEG
                     KGSLVQDLIDPLKAEMIDAAVFQVARESLSTSDYEQIPDRCMLSEEFIRKLIRLFQKT
                     INNEKIDEIVDSYFNSITKNEDFRFRY"
     gene            complement(6930..7223)
                     /locus_tag="VFC43_00090"
     CDS             complement(6930..7223)
                     /locus_tag="VFC43_00090"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HZK29624.1"
                     /translation="MKTIEIVISGKVQRVGFRACVRRIATDLHVSGTVINLPDGRVHI
                     IATAESIILEKFISMIYSCPRALIRDLRTSEISCMSFSDFSIIKGEGRLSTGI"
     gene            complement(7226..10783)
                     /locus_tag="VFC43_00095"
     CDS             complement(7226..10783)
                     /locus_tag="VFC43_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29625.1"
                     /translation="MVSSPEIIKKVQQALSEEDWKILEKKITAAQKDTGKRAQKTLIK
                     DINQLITTTSFKENKPELLLALPDLINNDIDDLFSQIIQQFIKTKDERWLKCVHVLSE
                     KLSKKSYQSRVIAMMAQALIGAGVYESNAKFIDNGIKLLEQISFQKYRSDIMIDIIPL
                     LIEWAITTRDEKLLYRSHLIIEEISDISKRAVLHAELAKALATVAILEKNRSLFLDGI
                     NSATKIHQKIRRQSCISSIIEKGVKSVFGKEMLDISTFIQNFDSLEYEKQLEIVHALT
                     EQLLDREKDKTRILAILQTVCEKMPYVTSTLVIDLLKKAELSGELWYLSAAVQLQERF
                     LKTEIYPVRDLVRAGISIAKHSNNMSVLSDLIPIIDKNSNTAIQSRIYLQFSEIMLSS
                     GDFTSGLDIFRKISNEAETLPQYEDNLTHLLKTGISRHSIPVVNEMILKRLNSETVNK
                     SINRAIIEISTENPFNDIISHIHSMNDLILLHPRQDHLLLESITILINRGFLTLHDPY
                     ILIRMAESIKEHHFKEEAISNIAVQIAKIGVKIKNRDYLQRAVGLTCDIDGQDIRSVT
                     LSGIIDEASNFAAQQGDLDLLLRMRVWCSSLLENNLAANAMPNIIEGVIKYAVDKKSP
                     ASLEEAYKIAKDISDPALKAQQFGRIAECFVIIGCILLNEPPLNRDPETFESEIYPFE
                     RGLAIIKEHVKKPRISLKIAGMIDILISYSNVSNNPDYAIPLAMYAVEIENPLERDAM
                     MSRIISNLHYNVTYPDSTDPYEIMASLLQRNVHSTSNPHMIVLIFRFLQLINDPYMEL
                     AGLCNLADTYIKIRDFDRARQILDDVRLSLSKIPDEFKKIFILSDLAILFSAIDPDKA
                     GKCLEQGIKRLDNVENDKKSVASKRIVLAIVRLNAIRPDSAFITIALEVSSKIADPVE
                     YVDSLIAVFNMVKNDKMRSSEILSRMSHAVEKIPSPYEKASALLEIIPLVMQNSDEEA
                     PVVLLKKAEALTKKINIPRIADTIRDKIAQMFVVFYQKYNNPKYLQNAIEVTKTIDDA
                     QIRLHRLTQMGQKSEFELPPHYEKINALSDKIIEDGGHPNQVTSLEKLIRSMTDRGKG
                     AILFCDLAVVFRTKGMEKISSRLLQSAIKEAQIIRPLSRRAFIMCDIAMKTHASGCEK
                     IAQEVLDLAIDAATNIRQSSLRDEVFDELGLAIKLMQGM"
     gene            complement(11061..12428)
                     /gene="glmM"
                     /locus_tag="VFC43_00100"
     CDS             complement(11061..12428)
                     /gene="glmM"
                     /locus_tag="VFC43_00100"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HZK29626.1"
                     /translation="MQTTKFQKRLFGTNGVRGVVGKDITPDLILDIGKALGTMRKGCI
                     AVGRDTRTSGEMLVKALKSGLLATGCDVVDCGILPTPALQYIIKDRFDGGAIITASHN
                     PPEYNGVKIIEADGTEMGDEETLKLEELIFDRAGSVKSWEGIGNEIIAHHLIRDYIDA
                     VVHHFPPLTGSGMTVVIDPGSGPACLTTPQILTAMGCRVITLNGIPDGTFPGRLPEPS
                     PAGLKGLSELVLSSGSAFGVAHDGDADRAVFIDENGQFVEENAEFSLVAQFLCHKKKG
                     AVVTPVSTSQMGELAVMNEGCTITYTPVGSIYVARTMRALMEKGNEVVFGGEGNGGLI
                     FPDHQFCRDGGMTAAMMVFILATTGQKISTLVSALPKRYIIKGKMSSSSGTQVLERLK
                     KIYSSYRLDLTDGVKIFQGPSWALVRTSGTEPIIRIIVDSDNENSGRVFYTEIMDHLS
                     KITHT"
     gene            complement(12443..12865)
                     /gene="mobB"
                     /locus_tag="VFC43_00105"
     CDS             complement(12443..12865)
                     /gene="mobB"
                     /locus_tag="VFC43_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HZK29627.1"
                     /translation="MKIIQIVGRSNSGKTTFIRTLIPELKKIGSVAVIKHLGDHLYNI
                     EEGKDTSVFFEAGADISVGIDDEKAVATIRKNTLDEALCMLLYQGMDFVIIEGFKQRS
                     FPKIAIGDLKTDNCILTNPLVKEVIESLDRFEDFIIKK"
     gene            complement(12928..13728)
                     /locus_tag="VFC43_00110"
     CDS             complement(12928..13728)
                     /locus_tag="VFC43_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5806 family protein"
                     /protein_id="HZK29628.1"
                     /translation="MDDSRIHQEVNKYKKFKKVEGATYRKVNQFLRKRTYITAREWAL
                     ARLCTDFRTSGGAEMTFIGNHLPELVPFMKDTYSPQAVNQARNSFKKKVRKASATFFY
                     GAMCGFFTTDELDDLLFEASEVARFLLEVEGTTLDIDEEIDIEDRITKVMRSVGEKAK
                     TILKTRAEEEDPPKEIPFMPQQNSIIPDTASHSSKKPADTAEKDEKLKITAASDIPQS
                     APISSKSIFLVDGKNAMEGSIPKTDNAEGISFTNRMRKADKNKPDLGE"
BASE COUNT         3772 a         3153 c         3038 g         4230 t
ORIGIN      
        1 tcattggcag aactacgagt tttacagtca gccaatcgga gtttgacggc tatctcggta
       61 actggtaccg gcttaacagt gcaggaaaat cggatggcag tgcgttcaat gtcgcagacc
      121 cgcaacttga aattaaagct gaagatacaa cagttgcggt cgatccaact attacatgga
      181 taccatcagg tgatgatatc cggttcaaga tagatacgaa ccttgtacag atggcttccc
      241 agcgtgcttc tccccccctc ataaccataa aggttgaggg gccggatggt ggttcctaca
      301 catcgttgta taatgcagga ggatccccct cttccatagt tgatatcccg gtaacgacga
      361 gaccatttta caccgcctca atatggaaca tgggaaatcg cgaccggtac cctccgggaa
      421 cgtatacgta ctgggcagaa tgtaatgtaa acagaatgaa tgacaactat gacgtgaccg
      481 gtaaaacggt gagcagaaaa attaccctgc ttaatcaggg tgtgaaccct ctgatcacca
      541 agcctacgac agttcagacc acaaagaccc tgcaacctgc tactacccag ctgactacca
      601 ttcagacaac tcccgctgat acaggtacaa tagtgtcaac accttccctt agcgtatccc
      661 cgacaacaac cgctcctgca gttccgaccg ccggccagac aaaagctcca gggtttggag
      721 taactcttgc gatctttgcg attttttttg gattggctgc gtattttaag aaccattgag
      781 aatacccttt tttacactcc acaatctttt attatctttc ttacccattc cggagtatga
      841 acattgtaac aaccgttatc aaatcacgcc acagtgtgcg aaaattcaaa cccgatccgg
      901 tagatgataa ggtgatcagg gatgcaatcg aatgtgcagc ccatgcacct acagcaaaga
      961 atattcagcc atggctgttt ggcgttatta aaaacaagga catgctgaaa gcgatcgcac
     1021 aatctacgga caatggaaaa tttattgctg acgctccaat gtgcgttgcg gtatttgggg
     1081 aacgaaatga aaaatattat ctcgaggatt gttgcgcagc cactgagaac ctcattcttg
     1141 ctctccaggc atatggtatc aattcctgct gggtagccgg ggaaaaaaag ccatacgctg
     1201 aatcaataag aaccctgctt ggtgttcccg aaaaatatac gctggtttct ctcgttgctg
     1261 ccggatgtcc tcgggaatta actattgcca caaaaaaaga gcaaaaacag attgtgtttt
     1321 atgaagagtt taaaaaagaa taatttttgt ttatatcctt gcagacttga tattttcttt
     1381 gcaggctggt cataacagta accgcgaatg tgattccctg gctgcctgat gtacacaatt
     1441 tctttctcgt aattattaag catcagggaa atcacatcat taatcatgaa aaaagcggtt
     1501 ctgcaggttg cactcgatat tattgaactc aaaagggcat tgcagatcgc acaggaatcc
     1561 attgatggtg gtgcagactg gatcgaagca ggtactccgt taattaaaag cgagggaatg
     1621 caggccatcc gctcgatgag ggaccgtttt ccgggttcca taattgtcgg ggatatgaaa
     1681 gtcgctgata ccggcacgct tgaggttgag atggccgcaa aagcgggggc gaatatcgtc
     1741 tgcattcttg ctgatgcgga tgattctgta attgccgaat ccgtgagggc tgcacgcctg
     1801 tatggaatcc ggattatggc ggatttaatc aatgtcaggg atcccataaa acgtgctcct
     1861 gaacttgaag ccatgggggt ggatatcatt tgtgcacacg ttggtattga ccagcagatg
     1921 ataggtaaaa attccattga cctccttacc tccctttcag gtctcatcca tatccccctt
     1981 gctgtcgcag ggggaatcag cgctgctact gccggggatg cggtaaaatg tggtgcagat
     2041 attgtcatcg ttggtggctg gattgtccga tccgcagagg tatcccaaaa cacaagaaaa
     2101 atccgtgctg aaatggacga tcctttagta agaaccatag agaaaaaatc tcctgatgaa
     2161 gaaatacgct cgcttctttt gcaggtatca gcaccaaaca tcactgatgc gatgcaccgt
     2221 aaaggggcta tgtcaggaat tctatccatc tgtggtaatg tcaagatggt tggaaaagcg
     2281 gtcaccgtcc agactgttgc aggggactgg gcaaaacccg ttgaagccat cgatgttgcc
     2341 ggaaaaaatg aagtgattgt tatcaataat gatggtgcgg tacatatcgc tccgtggggc
     2401 gagcttgcca cgctcagttg tatgaaaaaa ggaatatccg gagtggtaat tgacggtgcc
     2461 gtgagggacg ttgatgatat cagggcaatg aaatatcccg tttttgcaaa agcgatagta
     2521 ccgaacgccg gtgaacccaa aggattcggt gagataaatg ctgaaattca gtgctgtggt
     2581 cagtcagtct gtccgggtga ctggattgtg ggagatgaaa gtggcgtggt ggtgatacct
     2641 tccgttcgtg cctatgaaat tgcacgaaga gctctggaag tcaggaaaaa tgaggaacgg
     2701 attcgggaag agatacaccg gggaagtact ctttcccagg tggcagaatt aatcaaatgg
     2761 gaaaagaagt aggatttttt aatggaaaac cgagcgctct agaagagttg caccggagtt
     2821 tttaatcttt tttatcgcct cgtccaccat gtctacacgg agaatgagca ctgccccctc
     2881 ttttccggaa tatgcgtagg aatactcgat atttactccg gcatccccca gcacttttgc
     2941 aacttcatac aggccacccg gctggtcttt catccgcacg gcaattacat cagtaaaggc
     3001 gacgttgaat ccgagagaaa agagcttgtc atgtgctttc tcaggatggt ccacaagcgc
     3061 ccgtaccacc ccaaacccat tcgcttcagc gatactgaaa gcaagaatat tgatattctc
     3121 ctcccccagc gcgtgtgcaa ttgccgcaag acgcccgggc cggttttcag aaaatatcga
     3181 aatctgcctg attacatact tttgcgtatc cattacaatg acctcttgtc cacaactctt
     3241 ttggcttttc cttcaaacct cggtaaagaa cccggttcaa cgagttcaac gttgacattc
     3301 acgttcagtg aattcccgag ccggtgctcg actttctccc tgacctgcat aagatcattg
     3361 attttatcac tgaatgattc cttgttcagt tcaacacgaa caagcatgtc atcaagagct
     3421 ccttttcgtt caacaacaat ctggaaatgc tgacctactt cgggaattgc catcagggcg
     3481 tattcaatct gggaggggaa tacattgatg cccctgatga tcagcatatc atcaaccctc
     3541 ccctggattc tctggacgcg gggatgtgtc ctgccgcatg cacagacctc gtcgtccatg
     3601 gtcgttatat cccctatgcg ataccggatc ataggaagtg cctctttctg aagaatagtg
     3661 atagtcagct cccccttctg gccaggttcc aggggctcac cagtcttggg atcaataatt
     3721 tcaaccagtg caatatctga ccagatgtga aatccttttt gctcaacgca ttcagtaaac
     3781 atgggtccgg aaagttcact ggtcccgtat atatcatatg ccttgatacc cagccagtct
     3841 tcgatacgtg tacgcatgcg gtctgtccag ggctcagcac ctaagacccc tgtacgaagc
     3901 tgggtgtctt tttttatgtc aacacccatc ttttccgcaa cttcaccgat atggaggagg
     3961 tacgaaggag tgcatgctat agctgttgca ccgagatcct gcatcagttc tatctgacgt
     4021 tcggtgtttc cgacacttgt gggaagtacc gtcgctccga ttctttctgc accgtaatgc
     4081 atcccgagtc cgccggtaaa cagcccatat ccgtaactga cctgaattac gtctcctctt
     4141 ccgaggccga tagacgtaag ggcacgggcg agtgatgttg tccagagatt cagatcattc
     4201 tgggtatatc ccactactgt cggtttgccg gttgtccctg atgaaacatg gtagcggaca
     4261 agttcctcct gcgatgaagt aaagatttta tcaggataat tatccctcag gtcacgtttg
     4321 aacatgaacg ggagtttctt cacgtcatcc agttccctta tgtcatctgg atggacatgt
     4381 tgctctttca tccggtcatg gtaaaaagga gagaaactgt aaagtctgta taccagactt
     4441 tttaataatt tatactgcat tctccggaga tcttctcttg gcatctcctc aattcgcgga
     4501 tcccagctct tcattacatc gcctctcttc gatcaattac tctttttgcc tttccctcaa
     4561 atctcgggag ggaacccggc tcaacaagtt tcactgttgt cctgagctcg agtgcatccc
     4621 tcagttcttt aacaactttt ttctggagtt ttgcaaggtc agccagctcg ccactgaagt
     4681 attcgcggtt aatctcaacc tcgacagtca tttcatccag atgatttatt ctgtcgatat
     4741 ataccataaa ttgattgcca acctcaggta tcccgaggag cacatgttct atctgtgaag
     4801 gaaatacatt gatgccgcga ataaccagca tatcatcact tctgccagtg attcttgcga
     4861 ttttctgcga tcgtctgcac aggcagccgt cctccatgag tactgtcaca tcacctgtgc
     4921 ggtagcggag aagtggcata gcttctttta ccagcggggt caccacaagt tcgccgcgtt
     4981 ccccatcgga taattgctct ccggtctttg ggtcaatgat ttcgacaaaa aaactatcat
     5041 gccagatatg gagaccgttg cgctcggggc attcaaatgc aacaccggga ccaaataatt
     5101 cactcagtcc ataggaatca taggcagtca gaccgagtct tttttcaagc gaatgtcgta
     5161 cgctttcaga ccatggttca gcaccaaaaa gccctgttct tagactatca agggatgcat
     5221 tcatttcttc tgctatttcc gacaggtgca tggcatagct gggtgtacaa tggattgtgg
     5281 tcaccccaaa atccaaaatc atctcaatct ggcgttttgt attaccagta gcgcttggaa
     5341 ttacggtcat cccgattttt tcagctccgt agtgaaatcc aagccccccg gtgaacatgc
     5401 cataatttac catattctgg aaaatatcat ttttcctgac acccaccatg gtcatgttcc
     5461 tggctatgag atctgaccag gtatccaggt cgcgtcgcgt atacccgaca actgttggct
     5521 ttcccgttgt gcctgaagtc gtatgaatcc ggacaatttt tttcatgggg accgcaaaaa
     5581 aaccgaacgg gtaaccattc ctcagatcct gttttttcgt aaacggaatc ttctggatat
     5641 cgtcaagtgt ttttattgaa gatgttttca aacctgaacc ggccagaaga ttccggtaaa
     5701 actcaacatt ctggacctgg ctgagggttt ttttcaatcg tttcaactga agcgttttga
     5761 gttcttctgg tttcattgtt tcaagtttct tattccagaa catggaatgc ccctgttatg
     5821 ctatcgcata tcagacaata tatgtcgttt ggtactgttt aaattcaccg aatctgcctg
     5881 cataacagcg aatttaaatt cccccgaaat gcccgtaagt caggcaggaa aatccgatat
     5941 gaaaaacgat gaattaatca ggaggaaatt gtgtagtaat tagatcagct gaaaaaaata
     6001 tcacaggagt gaaatactta cgaagtatcc ttggttgccg gtcagtggtt ttggtgcaca
     6061 cataaaatcc actcagaaaa aactgatcat tcagaaaaaa aatactcttg aagaatatcc
     6121 cctggactcg ataaaaaacc tgcttgtaat cgggggtcat accatcagtt catcaacgat
     6181 cgtaagtctg gtaaaaaacg gggcatttat ctcttttttt gaaccagatg gcaatcctgt
     6241 cggtattatc cgtccgtttg gagaccagaa tgacagggat gtctacacat ggcaacaggg
     6301 agtcccccgt caccggtatg cgattattct ggcacaggct gcaattaaat cccgtctgtt
     6361 cgcaattcaa cgtgttcagg aaatccggaa tacgcatttg ttctataaag gcgagcagga
     6421 atttcttcat gcctcgcagg atgaattaca atacctgatc aaactggatg aaatccggag
     6481 attgcaccgg ttaacttcgg atatgtatta cgagatcatg tcccggaata tttcttcgga
     6541 ttttgggttc gggagaagaa ccatccggcc tcaggcagat ccagtcaatg caatgctgtc
     6601 atttggctac gcgatgctct atgggaactg ctgtgtatcg gttatcgggg cccgccttaa
     6661 cccggatgtc ggcctgatgc atgaaggaaa aggaagcctt gtgcaggacc tgattgatcc
     6721 tttaaaagca gaaatgatcg atgcagcggt atttcaggtt gccagagaat ctctcagtac
     6781 ttctgattac gaacagatac ccgaccggtg tatgctctca gaagagttca ttcggaagct
     6841 tatcagatta ttccaaaaaa ccatcaataa cgaaaaaatt gatgaaattg tggatagtta
     6901 ctttaattcc ataactaaaa atgaggattt cagattccgg tactgagtcg tccttctccc
     6961 ttgatgatgg aaaaatcgct aaagctcata catgatatct cagatgtcct caaatcacgg
     7021 atcagagcac ggggacagct ataaatcatg gaaataaatt tttctagaat tatcgattct
     7081 gccgtcgcaa tgatatgaac tctcccgtca gggagattaa tgactgtacc ggaaacgtgt
     7141 aaatctgttg ctattctccg tacacaggcc ctgaatccaa cccgctgaac tttcccgctg
     7201 ataacaatct caatagtctt cactgttaca ttccctgcat aagtttgata gcaagtccaa
     7261 gttcgtcaaa tacttcatcg cgaagtgaag actgacggat gttcgtagca gcgtcaattg
     7321 ccaggtcgag aacttcctgg gcaatctttt cacatccgga agcatgggtt ttcattgcaa
     7381 tatcacacat gataaatgca cgacgggaaa gtggccggat tatctgtgct tcttttatcg
     7441 cactctgaag caacctggaa gatattttct ccataccttt tgttctgaat actaccgcga
     7501 gatcgcaaaa gaggatcgct cctttaccac gatctgtcat tgagcgtatc agtttctcaa
     7561 gcgaagtaac ctgatttggg tggcccccat cctcgattat tttatcagac agagcattga
     7621 ttttctcgta gtgagggggg agttcgaatt cacttttttg ccccatttgt gtcaggcgat
     7681 ggagccttat ttgtgcatca tcaattgttt ttgtaacttc aatcgcattt tgcaggtatt
     7741 ttggattatt atacttttga tagaagacaa caaacatctg tgcaatttta tcgcgtatcg
     7801 tgtcagcgat ccggggtatg tttatctttt ttgtgagtgc ttctgctttc tttagtaaaa
     7861 cgacgggtgc ttcttcatcg ctgttttgca ttacaagggg tattatctcc agaagagccg
     7921 atgctttttc atacggtgaa ggaatttttt caaccgcgtg tgacatgcgt gataatattt
     7981 cagatgacct cattttgtcg ttttttacca tattgaaaac agcaataagc gaatcgacgt
     8041 attctacagg atcggcaatt tttgacgata cttcgagcgc aatcgtaatg aatgcactat
     8101 caggccgtat tgcattcaat cggacgattg ccagcacaat tcgtttgctt gctactgatt
     8161 ttttgtcatt ttcgacatta tccagacgtt taattccctg ttcaagacac tttcctgctt
     8221 tatcagggtc tattgcagaa aacagaattg ccagatctga aaggatgaaa attttcttga
     8281 attcatccgg aatttttgat aatgacagtc tgacgtcatc taaaatctga cgtgcacgat
     8341 cgaagtcccg tatcttgata tacgtgtctg ccaggttgca taaccccgca agctccatat
     8401 aaggatcatt aatcagctgg aggaaacgga atatcagaac aatcatgtga ggatttgatg
     8461 ttgaatgcac gttccgctgg agaagggaag ccataatttc gtatggatcg gttgagtccg
     8521 ggtatgtcac attatagtga agatttgaaa taatccggga catcatggcg tctctctcaa
     8581 gcggattttc aatttctaca gcatacattg ccaggggtat tgcataatcc gggttattgc
     8641 tgacatttga ataggagatg agaatatcaa tcattccggc aatctttaat gagatccggg
     8701 gcttttttac atgctccttt atgattgcca gacctcgttc aaacggatat atttcagatt
     8761 caaaggtttc agggtctctg ttgaggggag gctcgtttaa cagaatgcac ccgattatta
     8821 caaaacactc tgcaattctc ccgaattgtt gggcttttaa cgcaggatca ctaatatctt
     8881 tggcgatttt atatgcctct tcaagcgaag ccggagattt tttgtcaact gcatatttta
     8941 ttaccccctc aattatgttc ggcatagcat tagcggcgag attattttcg agcaatgagc
     9001 tgcaccacac tctcatccgc aacaggaggt cgaggtctcc ctgctgggcg gcaaaattgg
     9061 aagcctcatc aattattcca ctcagtgtaa cagaacggat atcctgacca tcaatatcac
     9121 acgtgaggcc tactgctctc tggaggtagt cgcgattttt tatctttacc ccgatttttg
     9181 caatctgaac agcgatattc gatattgcct cttccttaaa atgatgctct tttatggatt
     9241 cggccatcct gatcaggatg tacgggtcgt gtaatgtgag aaaaccccgg ttaatgagaa
     9301 tcgtaatact ctctaaaagc agatgatcct gccggggatg caggagaatg aggtcattca
     9361 ttgaatgaat atgcgaaata atatcattga acggattttc cgtgcttatt tcaataattg
     9421 cccgattaat tgatttattc acggtttcgc tgttcaaacg tttaagaatc atctcattga
     9481 cgacgggtat gctgtgcctg gaaattccgg tcttaaggag atgggtgaga ttgtcctcat
     9541 actgagggag tgtttcagcc tcattactga tttttctgaa tatgtccaga ccagaagtga
     9601 aatccccgga tgagagcata atctcggaaa actgaagata aatgcgtgac tggatggcag
     9661 tattgctgtt tttatcgata atcgggataa ggtctgaaag tacagacata ttgtttgagt
     9721 gttttgcaat ggaaatacca gccctcacca ggtcccttac aggataaatt tcagtcttta
     9781 agaagcgctc ttgaagctgt accgcagcag aaaggtacca caactccccg gataattctg
     9841 cttttttcag aagatcaatg acaagtgtgg aagttacata aggcattttt tcacaaacag
     9901 tctggagaat ggcgagaatc cgggttttat ctttttcccg gtctaacagc tgttcggtaa
     9961 gggcatgaac aatttcaagt tgtttttcgt attccagcga atcaaaattc tggataaagg
    10021 ttgatatatc cagcatttct tttccgaaca cggatttcac tcctttctca ataatcgatg
    10081 aaatacatga ctgtcttcgg atcttctggt gaatttttgt tgcactgtta ataccgtcta
    10141 aaaacaagga ccggtttttt tcaagaatag cgactgttgc caatgccttc gcaagctccg
    10201 catggagtac cgcacgtttt gaaatatcgc ttatttcttc aatgataaga tgtgaccggt
    10261 agaggagttt ttcatcccgt gtagttattg cccattcgat aagaagggga ataatatcga
    10321 tcataatatc tgatcggtat ttctgaaaac ttatttgttc cagcagtttt atgccgttat
    10381 cgataaattt tgcatttgat tcatagactc cggcaccaat aagggcctgt gccatcatgg
    10441 caattacccg ggactgataa ctttttttac ttaatttttc tgagagaaca tgaacgcact
    10501 tcagccatct ttcatctttt gtttttatga actgctggat aatttgcgag aatagatcgt
    10561 caatgtcgtt attgataaga tccggaaggg caagtaacag ttcaggttta ttttccttaa
    10621 acgatgtagt ggtaatcagt tggttaatgt ccttgatgag cgttttttgc gcacgctttc
    10681 cagtatcctt ctgggctgct gttattttct tttccagaat tttccaatct tcttccgata
    10741 acgcctgctg tacttttttg ataatttccg gagagctgac cattactata tcactggtat
    10801 gtatctatgg gataataaac acaatttaac tcaaatggtt attaaaattt ttgtttctat
    10861 tcaggcttga ttttattttt ttttatcata atggaacaaa attttcgatt ttttcctcat
    10921 tatcttggaa tatttaaatc atttttcagt aatcggcaaa attatgaatt attatcaaat
    10981 ttttcccgaa tttttccctg aaacggattt tttctgactc ccaggtcccg atctcgattg
    11041 cccgataaaa aactcatcca ttacgtatgg gtaatttttg aaaggtggtc catgatttct
    11101 gtataaaaaa ccctgccaga gttttcatta tcagaatcaa caataatcct gatgattggt
    11161 tcagttcctg aagtacgcac cagcgcccat gacggtccct ggaaaatttt tactccgtca
    11221 gtcaagtcca atcggtatga ggaatatatt tttttaagac gttcaagcac ctgagttccg
    11281 ctggaggaac tcattttacc cttgataata taccgtttcg gcaatgcact taccagtgtt
    11341 gagatttttt gaccggtcgt agcgagaata aaaaccatca ttgcagcagt catccctccg
    11401 tctctgcaga actggtgatc gggaaaaatc agacccccgt tcccttcacc tccgaatacc
    11461 acttcatttc ccttctccat gagtgcccgc attgtccgtg caacatagat acttccaacc
    11521 ggtgtgtagg ttattgtaca tccttcattc atcactgcaa gctcccccat ctgggaagtg
    11581 cttacaggag taacgacagc accttttttc ttatggcata aaaattgtgc aaccagggaa
    11641 aattctgcgt tttcttcaac aaattgtcca ttttcatcaa tgaataccgc acgatctgca
    11701 tctccgtcat gtgcaacgcc aaatgcagat ccgctgctga gtacaagctc tgatagtcct
    11761 ttgagtcctg caggtgaagg ttcggggagc ctgccgggaa atgttccgtc aggtattcca
    11821 ttgagtgtga ttaccctgca tcccattgca gtaaggatct ggggggttgt gaggcacgcg
    11881 ggaccggagc cgggatcaat caccacggtc attcctgatc ctgtaagtgg aggaaaatgg
    11941 tgcacaacgg catcgatata atctctgata aggtgatgtg cgattatttc gttaccaatc
    12001 ccttcccatg atttaaccga tccggcacgg tcgaatatca gttcttcgag tttcagcgtc
    12061 tcttcatcac ccatttccgt tccatcggcc tcaattattt ttacgccatt atattcggga
    12121 ggattatgag acgcagttat tatagcaccc ccgtcaaacc ggtccttgat gatgtactga
    12181 agtgcgggtg tcggcaggat gccacaatca acaacatcgc agcctgtcgc aagcaaaccc
    12241 gattttaggg ctttaaccag catttcaccc gaagtccggg tatctcttcc tacggcaatg
    12301 cacccttttc gcattgttcc gagtgctttc ccaatgtcga ggatgagatc tggtgttata
    12361 tctttaccaa ctactccacg aacaccattg gtaccaaata accgtttttg aaattttgtt
    12421 gtttgcatgg aacacgttcc ttttattttt ttatgataaa atcttcgaac ctgtcgagtg
    12481 attcgatgac ctctttgacc agaggattgg taagaataca attatctgtt ttcaaatcgc
    12541 ctattgcaat tttgggaaat gatcgctgtt taaatccttc aattatgaca aagtccatac
    12601 cttgataaag gagcatgcat agtgcctcat caagggtgtt tttgcggatt gtggctactg
    12661 ctttctcgtc atcgattccg acagatatgt ctgctcctgc ctcaaaaaaa accgaggtgt
    12721 cttttccttc ctcgatattg tataggtgat cacccaggtg tttgattact gcgacactgc
    12781 caattttctt cagttccgga atcagggtcc ttataaacgt tgtttttcct gaatttgatc
    12841 ttccaacaat ctgaataatt ttcatacggg agcacaacct ttttcaaact aattcggata
    12901 cagaaatttt cctgatatta tcgctcatta ttctcctagg tctggtttgt ttttatctgc
    12961 ttttctcatc cgatttgtaa aagatatacc ctctgcatta tctgtttttg gtatagaacc
    13021 ctccattgca ttttttccat caaccaaaaa tatacttttt gaagatattg gtgctgattg
    13081 cgggatgtcc gaagcagccg taatcttgag tttttcatct ttttcagctg tgtctgcagg
    13141 ttttttggat gaatgagaag cagtatcggg aattattgaa ttttgttgtg gcataaaagg
    13201 aatttctttg ggtggatcct cctcttcagc ccgtgtcttg agaattgttt ttgctttttc
    13261 tccgacactt ctcataactt tggtgatacg gtcctcaata tctatctctt catcaatatc
    13321 cagagttgta ccttcaactt cgaggagaaa tcgtgcgacc tcgcttgctt caaataaaag
    13381 atcatccagc tcatctgtcg tgaaaaatcc gcacattgcc ccataaaaaa aagtagcact
    13441 tgcttttctt actttctttt tgaatgaatt acgtgcctgg ttgactgcct gtggagaata
    13501 ggtgtctttc ataaaaggaa cgagttcggg aaggtggttt ccgataaaag tcatctctgc
    13561 acccccggat gtccggaaat ccgtgcagag gcgtgcaagt gcccattccc gggcggttat
    13621 gtacgttctc ttccggagga actggtttac tttccggtac gtagcacctt cgactttttt
    13681 aaatttttta tatttgttaa cttcctgatg gatacgcgaa tcatccattt tgggtgaaca
    13741 cctgctttga ttattggtta ttgatgatct acgacaagag cctgatgcgg acttgtcctt
    13801 gagatctaac tttgtgtata gcattatatg gtttattggt attaaaatat cgataaatct
    13861 ttcaggttta ttatattttt tttacgacgg aagagaggaa ttttacaaaa attacccgtg
    13921 caaagagcaa aaaaagtccg tgcacagatc ataatcggta tccccaattc catgaagaga
    13981 aaacaaaact ccttccggat aaattttttt attactatgg aacagtcctc aggataacga
    14041 gaattactta aaacttggat gggaaaaaca aggtattttt caaaaatcag agcttattct
    14101 cattaatatg tgaatgatca ggcgatttct tacctggaga gaccagcact aacacacctg
    14161 tatcaatctt ttatttttct cccagtgtta cgt
//
LOCUS       DASRLV010000003         3655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_732719,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000003 DASRLV010000000
VERSION     DASRLV010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732719"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VFC43_00115"
     CDS             <1..710
                     /locus_tag="VFC43_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZK29629.1"
                     /translation="DVKKPYLSGSMEGVIRFNTVKLEMTSLNGLKIQMEKDIKFLQPP
                     TGITLTPVGSFYLFTTLINGLVSSKEAMTLLGFVLVFVFLALVYRHVHAVTPLVPIIF
                     IVGWNAVAMYILGIAYTPLTATLGSMTIGVAAEYTILVMERYAEEEERLHDHIAAIQE
                     SVNKIGTAITISGLATFFGFSALCLANFPIISNFGITTLIAVGFSLMGAIFIMPAILS
                     VMGDLTERLEKRKKQPE"
     gene            804..1529
                     /locus_tag="VFC43_00120"
     CDS             804..1529
                     /locus_tag="VFC43_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK29630.1"
                     /translation="MPSVLEAKNLTKRYGDVIAVDNVSLFVEQGALFGLLGPNGSGKT
                     TMIKMLTGQTRPTEGSATVLGLEVTKDPVGVRGLVGIIPEQETPPSFLTAKEYLEFVA
                     AVRKIPDIETKADWWFDFLDFADKKNVLCKDLSRGTRQKLMFTQAFIHEPAVALIDEP
                     LINFDPIMQDLVKDYLAGYVKKGKTIFISTHILEVAEEICSGFAILHKGKLLHSGPVT
                     ELTSRNEHLPSFFLSLVRKDRHV"
     gene            1522..2928
                     /locus_tag="VFC43_00125"
     CDS             1522..2928
                     /locus_tag="VFC43_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29631.1"
                     /translation="MFELFTNMVKEEWRVHSTMFGSLSFALFPVMIFGIAFMGSFLIP
                     LMRTTLPSGNLTMIVHANYLMLGIMVGGFGLLGNEVMNRRFGQASFLAYSARSLPLSE
                     KFIFLNFVVKDTVYYFFLWVLPFGFGYILASPFIGVALATAFLLLLTLTLSFLFGLCG
                     VFFLSAIYARSRLLLWIVFLASGVGVGSLAVLTGTNPALFFPPLLLNSAFTWTNFLIS
                     CTVLTLLFGVAIILFNPESVGSEKMYNDSLAPLMMKFSVLPNPPFTAKDTIDLLRSGS
                     LVGQMIFSFLLPLAVIWFFLSLLGPFFPPHGLIFIFAITTGVIASTMYTWVTMFDTFG
                     PYACLPVAVSTLIVSKLTTFAVLQVIPAVFIAAVALLSGEAAYLIPAVILGISVSFYA
                     VGVMAWLCGLSPNVLVYDVKVLFVYLVLVGIVVTSFSSIAFANPNFAFLAVILAIPTW
                     LLIQKAKVRWDAVDPEGF"
     gene            complement(3013..3654)
                     /locus_tag="VFC43_00130"
     CDS             complement(3013..3654)
                     /locus_tag="VFC43_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HZK29632.1"
                     /translation="MKNGPYIVSGTIPLIKKEIRNDADGYCSTWHDVETYPLQEQYAL
                     CRCGHSKNKPFCDGTHTKIHFDGTETADDTPYLERAEKITGPALSLTDADNMCVHARF
                     CMRAGGIWNLTRQSGNPDARDLAIEEAGNCPSGRLVVWDRKTGEAIEPEFEKSIVIIE
                     YPPKSEHGPLWVRGGIPVESSEGHVYEIRNRVTLCRCGKSYNKPFCDGSHVER"
BASE COUNT          799 a          966 c          862 g         1028 t
ORIGIN      
        1 ccgatgttaa aaaaccttac ctgagcggtt ctatggaggg ggtcatacgt ttcaacacgg
       61 tcaagctcga gatgacatct ctgaatggcc tcaaaataca aatggaaaaa gatattaaat
      121 ttctccagcc gccgacagga atcactctta ccccggtagg atcgttttat ctctttacga
      181 cactcatcaa cgggctcgtg tcatcaaaag aggcgatgac cctgctcgga ttcgtgcttg
      241 tctttgtgtt cctcgcactg gtataccgtc atgtccacgc agttactcca cttgtcccga
      301 ttatttttat tgtaggttgg aacgcagtgg ccatgtatat actgggaatt gcgtatacgc
      361 cgcttaccgc aacactgggt tccatgacca ttggtgttgc cgctgaatat accatcctgg
      421 taatggaaag gtatgcagaa gaggaagagc gcctgcacga tcacattgct gccatacagg
      481 aaagtgtgaa caagattggt acagcgataa caatatccgg gcttgcaact ttcttcggat
      541 tttccgccct ctgtctcgcg aatttcccta tcatcagtaa tttcggtata actaccctga
      601 tagctgtcgg gttctcgttg atgggtgcga tcttcataat gcctgcgatt ctttctgtca
      661 tgggagatct gactgaacgc ctggaaaaaa gaaagaaaca acctgaataa atgaattaca
      721 tcataatttt tttagggatt gcataaccat gaggtattta ttcattgagt ttcgcacact
      781 agcataacag gcaggaacgg aaaatgccat ctgtacttga agctaaaaac ctcacaaaga
      841 ggtatgggga cgtaatcgct gttgataatg tctcgctttt tgttgagcag ggagcactgt
      901 tcggcctgct tggccctaac gggtcgggca aaaccaccat gatcaaaatg ctgacagggc
      961 agacccgacc gactgaaggt tcggcaaccg ttctcgggct cgaggtaaca aaggacccgg
     1021 tcggtgtccg gggactggtg gggataatcc ctgaacagga aacccctccc agtttcctca
     1081 ccgcaaaaga gtatctcgag ttcgttgcgg cagtccggaa aatccctgac attgagacaa
     1141 aagcagactg gtggttcgat tttctcgatt ttgccgataa aaagaatgtt ctctgcaaag
     1201 acctttcacg gggtacccgt cagaaactga tgttcacgca ggccttcatt cacgagccgg
     1261 cagtcgcgtt gattgatgag cccctgatca acttcgaccc gatcatgcag gatctcgtca
     1321 aggattacct tgccggatat gtcaaaaagg gtaagacaat ctttatctcg actcacatcc
     1381 tcgaggtggc tgaggagatc tgttcggggt ttgcgatcct ccacaaggga aaactgctcc
     1441 attccggccc ggttaccgaa ttgaccagca ggaatgagca cctgccttcc ttcttcctct
     1501 cgctcgtaag gaaggatcgc catgtttgaa ctgtttacca atatggtcaa agaggaatgg
     1561 cgggtccatt ccacgatgtt tggcagcctt tcatttgccc ttttcccggt catgatcttc
     1621 gggatcgcct ttatggggtc gttcctcatc cccctgatgc gaaccaccct cccgtccggc
     1681 aatcttacga tgatagtcca cgcaaactac ctgatgctcg ggatcatggt cggcggtttc
     1741 gggctgttag gtaacgaagt aatgaaccgt cgattcggcc aggcaagctt ccttgcttac
     1801 tctgcccgga gcctcccgct ttcagagaag ttcatcttcc tgaactttgt ggtcaaggat
     1861 acggtctatt atttcttcct ctgggtcctg ccctttggat ttgggtatat tctggcatcg
     1921 ccttttattg gtgttgctct tgcaacggca ttcctcctgc tactcaccct gacgctttcg
     1981 ttcctctttg gtctctgcgg tgtcttcttc ctctcggcaa tctatgcccg ttcccgactg
     2041 ttactctgga tcgtcttcct tgcgtcgggt gttggagtag gcagccttgc agtcctgaca
     2101 ggtacaaacc cggcgctctt tttcccgcct ctgttactta acagcgcctt cacgtggacg
     2161 aatttcctga tctcgtgcac tgtgctcaca ctcctttttg gggtcgcaat catcctgttc
     2221 aatcctgaat cagtgggatc agagaagatg tataatgact ccctggcccc attgatgatg
     2281 aaattttcag tcctgccaaa tcccccgttc accgccaaag atactatcga ccttttacgg
     2341 agcgggagcc ttgtcggcca gatgatcttc tcgttccttc tccccctcgc ggtaatctgg
     2401 ttcttcttat ccctgctcgg cccgttcttt ccgccccatg gtcttatctt catatttgcc
     2461 atcaccaccg gtgtaatcgc atcaacgatg tacacgtggg tcacgatgtt cgatactttc
     2521 ggtccgtacg cctgcctgcc ggttgcagtc agtacgctga ttgtgagcaa actaaccaca
     2581 tttgcagttc tccaggtgat cccggccgtt ttcatcgcag ctgttgcact cctctccgga
     2641 gaggctgcat atctcatccc tgccgtcata ctcgggatct cggtctcgtt ttacgctgtg
     2701 ggagttatgg cctggctctg cgggctctcc cccaatgtgc tggtttatga cgttaaagta
     2761 ctctttgtat atctcgtgct tgtaggcatt gtcgtgacaa gcttctcttc aattgccttt
     2821 gcaaacccga actttgcctt tttggcagtc atccttgcca tacccacctg gctgctgatc
     2881 caaaaggcga aggtccgttg ggacgcagtg gacccggaag gtttctgatt tttttaaaaa
     2941 aatgcccggg cggacgaaac ttcaggattt ttactacagc cgggccaaga aaaaaccaat
     3001 ttgccggcac cattatcgct cgacatggct gccgtcacag aacggtttat tgtacgattt
     3061 tccacaccgg cataatgtca cacggttccg gatttcatag acgtgccctt ccgatgattc
     3121 aaccggaatg ccaccccgta cccagagtgg accgtgttca ctttttgggg gatattctat
     3181 aatcacaatg gatttttcaa attcgggctc aattgcctct ccggtttttc tgtcccagac
     3241 aacgagacgc ccggacggac agttgcctgc ctcctctatg gcaaggtccc tagcatcagg
     3301 atttcctgat tgtcgcgtca ggttccagat cccaccggcc cgcatgcaaa atcttgcgtg
     3361 gacgcacata ttatcagcat cagtcagcga cagggcgggc ccggtaattt tttctgcccg
     3421 ttcgagatac ggtgtatcat cagccgtctc tgttccgtca aagtggattt ttgtatgggt
     3481 gccatcacag aagggtttgt ttttcgaatg tccgcaccgg cacagggcgt attgttcctg
     3541 caggggatat gtctctacat catgccatgt actgcagtac ccgtcggcat cgttacggat
     3601 ctctttttta atgaggggaa tagttcctga aacgatatag ggaccatttt taatc
//
LOCUS       DASRLV010000004         8652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_652655,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000004 DASRLV010000000
VERSION     DASRLV010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 8652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8652
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652655"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..104)
                     /locus_tag="VFC43_00135"
     tRNA            complement(31..104)
                     /locus_tag="VFC43_00135"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(68..70),aa:Phe,seq:gaa)
     gene            212..1183
                     /locus_tag="VFC43_00140"
     CDS             212..1183
                     /locus_tag="VFC43_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HZK29633.1"
                     /translation="MPLQHKSANIEETGLANAVKSRTAHVVHITHNDLDAVGADAIHR
                     MKYGNEGVFTIWSSVGKYTSLFSLVASCEGKGDLLSISDLGYQRDTELIAKKAHMNGW
                     RIEWRDHHRWRDDEIENMKKEVSLLRVDTSVCACGIVARDLQPENPLAAEVARVVCDY
                     DLWNHTDPRSAVLGQVLQRKKNREHVRDCLIKGIFCDPLIGQEYAEIKAEMDAMMQQS
                     LRHTTILGKRYKIAFAPLYGYPSETAHFIRNERKTDIEVIIGKDGKFSLRSVPPISHL
                     IAREFGGGGHPNAAGGSFNFTVLERFTWWLLKKNRHFREFVAIADEH"
     gene            complement(1280..2071)
                     /locus_tag="VFC43_00145"
     CDS             complement(1280..2071)
                     /locus_tag="VFC43_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29634.1"
                     /translation="MKKRLKLATRAFGAEPVRPDIAVLAEWIAVNRGTTADIITYLLD
                     QSLAPQVSAGILAPCSGGRFYKNRIVASLLGVREGNATDEIGLDSRAVSEDAAHIIVH
                     KKGAWFAMPAPHLLGITDMFYHDESEWHDAIAGTYRTIMRSMRDAGISGHVLICDALS
                     EPEITALSHPKVFFFQPKPDRAGLSCLLEHQHEVVVSRKTLDTVLDLTNEFDIRKVII
                     MDPDEDSISRALSCLDPDHISAGGYCLDRCETYWKNLLETAVYTI"
     gene            2111..4099
                     /gene="metG"
                     /locus_tag="VFC43_00150"
     CDS             2111..4099
                     /gene="metG"
                     /locus_tag="VFC43_00150"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HZK29635.1"
                     /translation="MNLPPLLVTCGLPYTNGPCHLGHLRTYVPADAYVRYMRRAGEEV
                     VFVCGSDNHGTPIVVSAEEAGITPRELSERYHKHFDETFKRMDIRFDRFGMTDDPATH
                     HRTKTIVSELVRNGYVYKQIVHQAYCTKCRRFLPDRYVEGICPHCGTPARGDECDQGC
                     GRHLEPGEIKDPVCKVCQTKAEFRDQEHYFFKLSDFKDFLLSYLENLRGTSNARNYAI
                     GWIKDELHDWCITRTLEWGVKFPQRDDLVVYVWVDAPIGYIAFTEEWAETTGKDWKRY
                     WCGENRVTHFIGGDIIYHHSIFWPAMLKGAGYGVPHAIVASGMVKVDDHKFSKSRGYV
                     VWTNDDYLDKGLPSDYLRYYLLAYTSHTKELNFSWKIFSERINNEVVNILGNFVYRSL
                     FFAHKEWQGIPEGVADPTITAEIEKSLASVDRYMREYEFKGAVDAIMGLAAFGNTYIQ
                     TNAPWKLIKTDRAAAAQVVKNCVQITKALTLLIEPVMPQKAQECWNQLGYEDTLADHP
                     IAEATYPVLPARIQAPSPLFSRMEEAQVKDLDALLQKRVAEANKKMERIPVVTYEEFQ
                     KLDIRTGKVLFAEPVPKSNKLLKLQVDIGNETRQIVAGIQQFYNPDELVGREVVVVTN
                     LAPAKIFGVESNGMILAAGDSASLLVPLRSVESGSKIR"
     gene            complement(4268..4888)
                     /locus_tag="VFC43_00155"
     CDS             complement(4268..4888)
                     /locus_tag="VFC43_00155"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HZK29636.1"
                     /translation="MESGKGYTLPKLPFGYKALAPYISEEQLTLHHQKHHQAYVTGAN
                     AVLEKLDKARKDSIDLDMKAMLKELSFHIGGYRLHKLFWENMAPAGSGGGGLPKGELL
                     KAIDDEYGKFDRFKKEFTQAASGVEGSGWAVLTYCMTTGRPMILQLEKHNVNMVPGYR
                     ILMALDVWEHAYYLDYKNDRAKFIESFWNIVNWDEVNARFVTHMKK"
     gene            5082..6836
                     /locus_tag="VFC43_00160"
     CDS             5082..6836
                     /locus_tag="VFC43_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZK29637.1"
                     /translation="MNFLFLGQKYTNPRTVIGLIAASTACSLILNWYGLTLGITNVLP
                     HLLYIPIILTAYYYPRWGVAFTTGLSLAYGIIVWQFFLPGSDILFSALARILVFITIA
                     AVVSYLSGRLQHDANLSQRLASIVESSNDAIVGQTMDGIITEWNHGAERIYGYKPSEM
                     IGRRVNLLFPPEVPDELPRLIDKIFRGERIERFETERITKNGIRIQVSLSLSPIKNVQ
                     GQIVGISTTVNDISEKKRMQDQILRAKTEWELTFDAVPDLIAIIDQRNHILQVNKAMT
                     ERLGASREDIVGRACYQVVHHTMMPPGFCPHQMLLSDGKEHSLEIREERLKADFLVTA
                     SPLHDSHGMIIGSVHVMHDITERKRAETALQLALKKLNMLSSITRHDIQNQLMAMRAY
                     LGFLKEKITDPGLLGFVGKEETAIEAISRQIEFTQHYENIGVHAPQWQDVCAIIRSSA
                     SQLSTDTISLEVTFSGIEVFADPLIGKVFYNLMENSFRHGGNITRITFSFERMNGDAK
                     ITYRDDGTGIPVDDKEQIFRKGFGKHTGLGLFLTREILSITGITIRENGEPGKGVQFE
                     IVIPHDMHRISSDSKVAL"
     gene            6833..7714
                     /locus_tag="VFC43_00165"
     CDS             6833..7714
                     /locus_tag="VFC43_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HZK29638.1"
                     /translation="MTDKDPDPKLTSTLLALQKAEITEHDIYKKIAAIQKNAHNREIL
                     DQIAQQELEHYGIWKKYTNRDVAPNAIRIWFYYLMARLLGMTFAIKLMEGVEKRAQGY
                     DQALISQIPETRDMLKNEENHERELIALIDEERLKYVGSVVLGLNDALVEFCGTLAGL
                     TFAIQNTQIIAIAGLIMGVAASLSMGASEYLSQRSEAGSTDPVKASVYTGFTYMVTVA
                     ILILPFLVLSSPYIALLCTLLGAVVVIFLFTFYISVAKDLSFGKRFGEMLLISMGIAT
                     ISFVIGIFIRIGLNINV"
     gene            8019..8354
                     /locus_tag="VFC43_00170"
     CDS             8019..8354
                     /locus_tag="VFC43_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29639.1"
                     /translation="MVRTEREMVGIYGQVLLTTNPAPAGFFSRYILCVTPVLLVVISL
                     VVLEFMRILMSSFLPSWGKTLALIVPDLPVIMEIVVLCISPVGILLFLSTLAMWSNDM
                     KYGLDLPLR"
     gene            8473..>8652
                     /locus_tag="VFC43_00175"
     CDS             8473..>8652
                     /locus_tag="VFC43_00175"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HZK29640.1"
                     /translation="MAAVFILAGIEIFRRSVVYTITRDVVIITGGIGKQVENVIPLHN
                     VERILVVQGRMGRYFN"
BASE COUNT         2329 a         2020 c         2057 g         2246 t
ORIGIN      
        1 ctgataaatt ccaaaaaagc agcaaaagag tgccccaggc ctgatttgaa caggcgacaa
       61 ctagatcttc agtctagcgc tctcccaagc tgagctacta gggcaggagt acaattgttg
      121 tcactggtga ctaataaatg atatgctctg atgcttcgca ggatactaac tgaccgttct
      181 tttttatatg ccgcgatggt actattaact gatgccgttg cagcataaga gcgctaatat
      241 cgaagaaaca gggcttgcaa atgcggtgaa aagccggaca gcgcatgtgg tccatatcac
      301 tcataatgat cttgatgcag tcggggcaga cgcgatccac cggatgaaat atggaaatga
      361 aggtgtgttc acgatatgga gttcggttgg aaaatatacg tcactctttt ctcttgttgc
      421 ttcctgtgag gggaaaggtg atctgctctc gatctctgat ctcgggtacc agagggatac
      481 tgaactgatc gcaaaaaaag cacacatgaa cggatggaga attgaatggc gggatcatca
      541 ccgctggcgg gacgatgaga tcgaaaacat gaaaaaagaa gtatcattac tccgcgtcga
      601 cacttctgta tgcgcctgcg gaattgttgc ccgcgatctg cagcctgaaa accctcttgc
      661 cgccgaagtt gcacgggttg tctgcgatta cgatctctgg aatcatactg atccccggtc
      721 tgcagtcctc gggcaggtac tccagcgcaa aaagaaccgg gaacatgtcc gggactgcct
      781 cataaaaggg attttttgtg atccgctgat cgggcaggaa tacgccgaga taaaagctga
      841 gatggacgcg atgatgcagc aaagcctcag gcacacaacc atccttggca agaggtacaa
      901 aattgctttt gccccgctgt acggctaccc aagcgagact gcacatttta tccggaacga
      961 gagaaaaaca gatatcgaag tgatcatcgg gaaagacgga aagttttctc tccggtcagt
     1021 ccccccgata agccacctga tagcacgaga gtttggcgga gggggacatc cgaacgctgc
     1081 cggaggttcg ttcaatttta ccgtactgga acgctttacc tggtggctcc tgaagaaaaa
     1141 ccggcatttc agggaatttg tggccattgc ggatgaacat tagcttacca tacggtgttt
     1201 cctcatgtgt tttttttgat ttttcatcaa attcttccag cttgccacat tgttttttaa
     1261 gaaatcgttc ttttgatggt cagattgtgt aaactgccgt ttctaacagg tttttccagt
     1321 acgtttcgca cctgtccagg caatatcccc cggcgctgat atggtccgga tcaagacagg
     1381 aaagtgcacg tgaaatcgag tcctcgtcag gatccataat aattactttg cggatatcga
     1441 actcattggt aagatcgagt acggtgtcga gagttttcct gcttacgact acctcgtgct
     1501 ggtgctcaag caggcatgaa aggcctgccc gatctggttt aggctggaaa aaaaacactt
     1561 tcggatggga aagtgcagtt atctcaggtt ctgacagtgc atcacagatc agcacatgcc
     1621 cgcttatgcc agcatccctc atagaacgca taattgtccg gtaggttcct gcgattgcat
     1681 cgtgccactc ggattcatca tgatagaaca tatccgtaat gcccagaaga tgaggagctg
     1741 gcatggcaaa ccatgcccct tttttatgga caattatgtg tgcggcatct tcactgacag
     1801 cccgagaatc cagaccaatt tcatctgttg cattcccttc tctgactccg agcaggctcg
     1861 ccacaatccg gtttttgtaa aacctgccac cactacaggg cgccaatata cctgccgaaa
     1921 cctgaggtgc aagtgactgg tcaagcaagt acgtgataat atcagcagtt gttccacgat
     1981 tcactgcaat ccattcagca aggacggcga tatccggcct tacgggctct gctccgaatg
     2041 cccgtgttgc cagtttaagc cgttttttca tatacagatc ttattactta taagcatcta
     2101 aaaagtattg atgaacctac caccgctgct ggttacctgc gggttgccat acacaaacgg
     2161 cccctgtcac ctgggacatt tgcgaacgta cgttccggct gatgcatacg tccgctatat
     2221 gcgacgggca ggagaggaag tcgtttttgt ctgcggttcg gacaatcatg gtacacctat
     2281 tgttgtctcg gctgaagaag caggtatcac accccgggag ctctctgaac ggtatcacaa
     2341 acattttgat gagacattca aacggatgga tatccggttc gaccggttcg gcatgaccga
     2401 tgatcctgca acccatcacc gcacaaaaac aattgtgtcc gaactcgtca ggaatggcta
     2461 cgtgtataag cagattgtcc atcaggcgta ctgtacaaag tgcaggcggt ttcttcctga
     2521 cagatatgtt gaaggtatct gcccccactg cggaacaccc gcacgggggg atgagtgtga
     2581 ccaggggtgc gggaggcacc tcgaaccagg tgaaattaag gatcctgtct gcaaggtctg
     2641 ccagacaaag gctgagtttc gtgaccagga acattatttc tttaaacttt ccgattttaa
     2701 ggatttcctg ctctcttacc ttgaaaatct gaggggaacg agcaatgcca ggaattatgc
     2761 gatcggatgg ataaaagatg aacttcacga ctggtgcatc acccgtacac ttgaatgggg
     2821 ggtgaagttc cctcaacgtg acgaccttgt ggtatatgta tgggtcgatg ccccgatcgg
     2881 ttatatcgca ttcacggaag aatgggctga aacaaccgga aaagactgga agcgttactg
     2941 gtgcggtgaa aaccgggtca cgcattttat tgggggcgac atcatctatc accatagcat
     3001 tttctggcct gcgatgctga aaggagcagg ctacggtgtc ccccatgcga ttgtcgcaag
     3061 cggaatggtc aaggtagatg accataagtt ctcaaaatcg cggggctatg tagtatggac
     3121 caacgatgac tacctcgata aaggattgcc gtctgactac ctccggtact atcttttggc
     3181 atacaccagc cataccaaag agctgaactt ttcatggaag attttttccg agcggatcaa
     3241 taatgaggta gtcaatattt taggcaattt cgtgtatcgt tcccttttct ttgctcacaa
     3301 agaatggcag ggaatccctg agggtgtggc agatccgaca atcacagccg aaattgagaa
     3361 aagccttgcg tccgtggaca ggtacatgcg ggaatacgaa ttcaaaggag cggtagatgc
     3421 aattatgggg cttgcggcgt tcgggaatac ctacattcag acaaatgctc cctggaagct
     3481 cattaagacg gaccgggcag cagcggcaca ggtagtgaaa aactgcgtcc agataacaaa
     3541 agccttaaca ctcctcattg aaccggttat gccgcaaaaa gcacaagaat gctggaacca
     3601 gctgggctat gaagatactc tggcagatca tccgatcgcc gaggcaacgt atcccgtgct
     3661 tccagcccgt atccaagcac cgtccccgct cttttccaga atggaagaag cgcaggtgaa
     3721 agaccttgat gccctgctcc agaagcgtgt ggcagaggcc aataaaaaga tggagaggat
     3781 ccccgtggtt acttatgaag aattccagaa actggacatc cggacaggta aagtactttt
     3841 tgcagaacct gtaccaaaat ccaacaaact cctgaaactt caggtagata ttggcaatga
     3901 gacccgccag atcgttgctg gcatccagca gttctacaat cctgatgagc tggtcggcag
     3961 agaggtggtc gtagtcacta accttgctcc ggcaaaaata ttcggcgtcg agagcaatgg
     4021 aatgattctt gccgcagggg actctgcatc gctgctcgta ccgctccggt ccgtggaatc
     4081 cggatcgaag atccgttaaa atcccggtat tatttttttc aggcgagttc ttcaacggat
     4141 aattatatca ccaaaaaaac gaggttcatg acagaacagt tgatcgctcc tgtaaaaagt
     4201 attgcgatgg agtaaaaaac taaaaagtaa tggaaatggg aaatgaggag cacgaggggt
     4261 aaaggagtta ttttttcata tgcgtgacaa atcgcgcatt tacttcatcc cagttcacaa
     4321 tgttccagaa cgactcgata aattttgccc ggtcgttctt gtaatcgaga taatatgcat
     4381 gctcccagac atcgagagcc ataagaatcc gatatcccgg taccatattg acattatgct
     4441 tctcaagctg gagaatcatg gggcgtccgg tagtcatgca atacgtgagc accgcccatc
     4501 cggaaccttc gacacctgag gctgcctggg taaactcttt tttgaaccgg tcaaattttc
     4561 catactcatc atcaattgct tttaataact ccccttttgg taatcctcca ccaccactcc
     4621 cggcgggtgc catattctcc cagaagagct tatgcagacg atatcctccg atatggaagg
     4681 acagttcctt tagcattgct ttcatgtcca gatcgatgct atccttacgc gccttatcga
     4741 gcttttccaa cacggcgttt gcacctgtca catatgcctg gtgatgtttc tggtgatgca
     4801 aggtgagttg ttcttcagag atatacggcg ccagagcttt atacccgaag ggtagttttg
     4861 gtaaggtgta acctttgcct gattccatcg tcatgacctc cttagtggat aattgtgtat
     4921 tagtatgttt ccggtatttg tttattgtac aattaccaaa cagggttgca gcgtagggta
     4981 atatatgcaa aatatcggga atacatctgt gataagttaa aaagcgaagc atagattaaa
     5041 cgccgggtaa atattgttta tcgggaccag gagtatccac catgaatttc ttatttcttg
     5101 gccagaaata cacaaatcca cgaacggtta tcggcttgat cgctgccagt accgcctgct
     5161 cacttatcct gaactggtat gggctcaccc tcggtattac caatgtatta ccacaccttt
     5221 tgtatatacc catcatcctc acggcgtatt attatccccg ttggggtgtt gcctttacta
     5281 cggggctctc attagcgtac ggcattatag tctggcaatt cttcttaccg ggctctgata
     5341 tactcttttc cgctcttgca cgcatcctgg tattcatcac cattgcagcg gtggtatcct
     5401 atttgagcgg acgtctgcag catgatgcaa atttgtccca gcgactcgca tctattgttg
     5461 agtcttcaaa tgacgcaatt gtcggacaaa ccatggacgg gatcataacc gaatggaacc
     5521 acggagccga gagaatctac ggatataaac ccagtgaaat gattggcaga cgggtcaatc
     5581 ttctttttcc cccggaggtt cctgacgaac tccccaggct cattgataaa atttttcgcg
     5641 gggaacgcat tgagcggttt gaaacagagc ggataaccaa gaacggaatc cgtatacagg
     5701 tctccctctc cctgtccccg ataaaaaacg tccaggggca gattgtgggc atatcaacca
     5761 cggtcaatga tatctccgaa aaaaaacgga tgcaggacca gatcctgcgt gcaaagaccg
     5821 aatgggagct cacattcgat gcggttcctg atctaatcgc aattattgac cagagaaatc
     5881 atatcctgca ggtcaacaag gcgatgacag aacggcttgg agcttcccgt gaggatatag
     5941 taggacgtgc gtgttaccaa gtcgtgcatc acaccatgat gcccccgggg ttctgtcctc
     6001 accagatgct gctttcagat ggaaaagagc actcattaga gatccgtgag gagaggctga
     6061 aggcggattt tctcgtaact gcatcccccc ttcacgattc acacggaatg ataattggca
     6121 gcgtccatgt catgcatgat attaccgaac gaaaacgtgc ggagactgct ttgcaactgg
     6181 cactgaaaaa actgaatatg ctttcatcca ttacccggca tgatatacag aaccagttga
     6241 tggcaatgcg cgcatatctt ggatttctca aagagaaaat aactgatccc ggtcttcttg
     6301 gtttcgtcgg taaagaggaa acagcgattg aggcgataag cagacagatt gaatttaccc
     6361 agcactatga aaacatcggt gtacatgcac cccaatggca ggacgtctgc gcaatcatcc
     6421 gatcatccgc atcgcaattg tctaccgata caatttctct tgaagtcacg ttttccggca
     6481 ttgaagtgtt cgccgaccct ctcatcggta aagtgtttta taatcttatg gaaaattcct
     6541 tccgacatgg aggaaacatt acccggatca cgttctcatt cgaaagaatg aatggtgatg
     6601 cgaaaattac gtaccgtgat gatggtactg gtataccggt ggatgataaa gaacaaatct
     6661 ttagaaaagg gtttggcaaa cataccggcc ttggactgtt cttaacccgc gagatccttt
     6721 cgattaccgg tattaccata cgtgagaatg gtgagccggg caaaggggtg cagttcgaga
     6781 tagtgatacc tcatgatatg cacaggatat cttctgattc aaaggttgcc ttatgacgga
     6841 caaagacccc gacccaaaac ttacctcaac acttcttgcc ctgcaaaagg cagaaattac
     6901 ggagcacgat atctataaaa aaattgctgc aatccagaag aatgcgcaca accgtgagat
     6961 acttgaccag atcgcacagc aggaacttga acactatggc atctggaaaa agtacacaaa
     7021 cagggatgtg gcaccgaatg cgatccgtat ctggttttat taccttatgg cacgcctcct
     7081 gggaatgacc tttgctatca aactcatgga gggagtggaa aaacgtgccc agggatacga
     7141 tcaggcactt atctctcaga ttcccgaaac ccgggatatg ttaaaaaatg aggaaaatca
     7201 tgaaagagaa ctgattgcgc tcatcgatga agagcggctg aaatacgtag gatcggtcgt
     7261 ccttggcctc aatgatgctc ttgtcgaatt ttgcggtaca cttgccggcc tcacttttgc
     7321 catacagaat acccagataa tcgcaattgc cggcctgatc atgggagtcg cagcctcact
     7381 ttcaatgggg gcatccgagt acctttcgca gcggtctgaa gcaggatcaa cagacccggt
     7441 caaggcatcg gtgtataccg gattcaccta tatggtaact gtcgccattc tgatccttcc
     7501 gttcctggta ctgagcagcc cgtatattgc cctcctgtgc accctcctgg gagcagtcgt
     7561 tgtgatattc ctgttcacct tctacatatc cgttgcaaaa gacctctctt ttgggaaacg
     7621 gttcggggag atgctcctta taagcatggg aattgcgacg atatcatttg tcatcggtat
     7681 attcatccga atcggtctga acataaatgt ctgaggattt taatgcggat ttcaggatgc
     7741 atattttatt ttttaaacaa aacctcatta aggaccatca gcgactgaag gaagggtaaa
     7801 aatcgttttc ccccaacata attcctccaa ccaaaacaac cgcaaggctt tttaaaaacc
     7861 tgtcaccagt tcatctctta tgaatctcga aaattgacag gacgcagatt taatggagat
     7921 aaaatccata ttttgtttta atttccctca aatttttgta aaagagatta tttcaaaaga
     7981 gaaacttata aataaatgtt gaaataaaga gccgatacat ggtgagaaca gaacgtgaga
     8041 tggtgggaat ttacggacag gttcttctca cgaccaaccc ggcaccggca ggttttttct
     8101 ccagatacat cctttgtgtg actcctgtcc tgcttgtcgt gatctctctt gtggtccttg
     8161 aattcatgcg gattcttatg agttcatttc ttccgtcatg gggaaagact ctggcattga
     8221 tagttccgga cctccccgta attatggaaa tagtagtact atgtatttcg ccagtaggaa
     8281 ttctcctttt tttatctacc ttggcgatgt ggtccaacga catgaaatat ggattggatc
     8341 tgcccttacg ttaattttga gtatactggg tgccctgttt tttttacagg gtacggatat
     8401 accggtcctt tccacaagat acctgagact cttttacaat gggtagcgta cctggttcaa
     8461 cccgcgagcg tgatggctgc cgtattcatt ctcgcaggaa ttgaaatatt caggcgctcg
     8521 gttgtatata ctattacccg ggatgtggta ataatcaccg gtggtatagg gaaacaggtt
     8581 gagaatgtca taccacttca taatgttgag agaatccttg tcgtgcaggg tcggatgggt
     8641 cgatatttca ac
//
LOCUS       DASRLV010000005         5922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1057866,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000005 DASRLV010000000
VERSION     DASRLV010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5922
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057866"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VFC43_00180"
     CDS             complement(<1..676)
                     /locus_tag="VFC43_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3458 domain-containing protein"
                     /protein_id="HZK29641.1"
                     /translation="MDLIFDVFDNHTKVTADLQAESRDSPLSEVVLNAKNLEIIAVNC
                     PGHTCSHAYDVAQSALTIRFEDPVLPLTRFTLHTETICRPTENILEGLYYDKTPPGAP
                     PQQITQCQQWGFQRLVPCIDDMTAKCTYTTTIIADERYTNLITNGNVIQPRQPAGNGR
                     VKITCENSATPMAPYLFFLGCGTYATFSQECEYPDGRQCTLELLVPPASDPVTAGIAL
                     DILHDAV"
     gene            1173..2159
                     /locus_tag="VFC43_00185"
     CDS             1173..2159
                     /locus_tag="VFC43_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZK29642.1"
                     /translation="MTIAIHIENLTRRYTDLVAVDNISFDIEQGEIFGLLGPNGAGKT
                     TTLSMLSTMLKPTSGSATINGIDIERDEDGVRKSIGIVFQDQSLDEELTAWENMDFHG
                     RLYRIPADIRKQRITELLRLVELEDRKDDIVKTFSGGMRRRLEIARGLLHHPSVLFLD
                     EPTLGLDPQTRNHLWQYIATLAKEKGITIILTTHYMEEADRLCNRVAIIDHGKIIALD
                     TPQNLKDGIGGDVVTIRSPDPSAIVAALAEPWITRVEQHNDEVIVSLQNAEQHISTIV
                     TLLNKRQVPIQSIAIHKPTLEDVFLSFTGKTIREEEADHKTNMRMYQRMVRH"
     gene            2162..2911
                     /locus_tag="VFC43_00190"
     CDS             2162..2911
                     /locus_tag="VFC43_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK29643.1"
                     /translation="MDIIYTIWLRNMKRYIRSKSRIIGSISMPLFFLLFLGFGLNSVV
                     QLPGMGGNYIVFLIPGMVAMSVLFTSVFSGIQIIWDKQFGFLKETLVAPVSRLEIMLG
                     QTLGGATTAVLQGFMILVLSVFIGLKISNPAGFLIALGFMILIGISFTAFGIAIASRM
                     EDMTGFQLIMNFVVFPIFGLSGALFPISSLPSWMGPVTLLDPLTYGVEGIRYGLTGVS
                     QINPLVCAAVIGGFTVVMTVLGAFLFRKIKF"
     gene            3391..4215
                     /locus_tag="VFC43_00195"
     CDS             3391..4215
                     /locus_tag="VFC43_00195"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HZK29644.1"
                     /translation="MQPKKKTKKHPLLPVLAGSMLLIPFLLAAGCTGQVPADSRLNGT
                     GWTLTWYAQNGTLSQSLALTKVTLDFVNYGQITGSAGCNHYFASYKVDGTAITFGQAG
                     STEMYCGTPGVMDQESAYLTLLGQAKTVTVEGDRLSFADAKGTTILTFIKTVPPAQEP
                     LVGTNWTLESFHTADAVSSVLAGTTITAVFGADGSVSGSAGCNRYFASYNITGTSLSI
                     GPAGSTKMACGAPGVMQQEGKYLTLISQAKTVIIKGGSLTLADAKGAAILSYAKEA"
     gene            complement(4343..5044)
                     /locus_tag="VFC43_00200"
     CDS             complement(4343..5044)
                     /locus_tag="VFC43_00200"
                     /inference="COORDINATES: protein motif:HMM:NF040120.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HZK29645.1"
                     /translation="MKFPQPLYRILPEKSDLTPPVISLLSANLITIVLAIAGNWDAAT
                     VIFIYWAQSIIIGIFTVVTILCADTRSISAAMNYSRAERGESGTVTPGSIRTQAVILG
                     AFFSVHYGIFHAGYYFFIVESGLFGVVNLSSPGIWYSCGLFFVNHLFSYLYYRNRLRQ
                     DKDFMTETFTAPYYRIIPMHFTIMFGAMVIIVLGIIGITSTLPVLVLFLLLKTATDMA
                     MHLQKHPGVQENKAR"
     gene            5303..5536
                     /locus_tag="VFC43_00205"
     CDS             5303..5536
                     /locus_tag="VFC43_00205"
                     /inference="COORDINATES: protein motif:HMM:NF018961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HZK29646.1"
                     /translation="MKDTHEKEHISVAKVVELIGSSPNSWEEATANAVEAAGQTIRNI
                     KGVDVKRCTAKVVNNKIVEYRAVVKIAFIVERE"
     gene            5702..>5922
                     /locus_tag="VFC43_00210"
     CDS             5702..>5922
                     /locus_tag="VFC43_00210"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZK29647.1"
                     /translation="MFPIGDDNTSRRLQPVVTYALIALNVLFFLIELMGGDTFIETWA
                     FVPSRFLANPGGDFLTIFTSMFMHAGWLH"
BASE COUNT         1475 a         1487 c         1526 g         1434 t
ORIGIN      
        1 gaacagcatc atggaggatg tccagggcaa tgcctgcggt gacgggatcg gatgccgggg
       61 gaaccagcag ttcgagtgtg cactgccgcc cgtccgggta ctcgcattcc tggctgaagg
      121 tcgcgtaggt cccgcacccg agaaagaaca ggtacggggc catcggtgtg gcactgttct
      181 cgcaggtaat tttcaccctg ccgtttccgg caggttgacg gggttgtatc acattcccgt
      241 ttgttatcag gtttgtatac cgttcgtcag caatgatcgt cgtggtgtac gtgcactttg
      301 ccgtcatgtc atcgatacac gggaccaggc gctggaaccc ccactgctgg cactgggtaa
      361 tctgctgggg cggtgcaccc ggcggggttt tgtcataata caggccttca agaatatttt
      421 ctgtcgggcg gcagattgtt tctgtatgca gggtgaaccg tgtcagcggc aaaaccggat
      481 cttcaaaccg gatcgtcagg gcgctttgtg caacatcgta tgcgtgggaa catgtatgcc
      541 cgggacagtt taccgcaata atttcaagat tttttgcatt cagcacaact tctgaaagcg
      601 gactgtcacg ggattcagct tgcagatcag ctgtcacttt cgtgtggtta tcaaaaacgt
      661 caaagataag gtccatgtgg aggactcgga tgggaatctt tccgaaatcc tctttatagt
      721 agcggaactg gcgactggtc atggcggttt ttggtttata tgctgccgaa ggtgctctct
      781 ttagaggtat ttatgcatat gtccatcttt caaaaaatgt aacaaaacag gcgctggaga
      841 attcctttta tcgggttatg aaataaaaaa acacaggtca gatgaaggag ggacaataat
      901 gtccagatta ccgcccctgt tctcaggaga gcgatgatga tggagtacct gttcaggtgg
      961 tattgcatcc ggggggagcc gcagcggcgg ggtgaaacac cagtggagcg ttcaggttat
     1021 tttaaacatt tctccagggc cccaaaaaac cgcttccagc ttttccgccg gatcgtctct
     1081 gaaatctggt actatgaaaa gtgtattaac agaacaggca tcaaccatac cggaacgagt
     1141 gtgtccaccg gggatggaca ggatcgcatc ttatgactat tgctatacat atcgaaaact
     1201 taacccggcg atataccgac ctggtcgccg tggacaatat atcatttgat atcgaacaag
     1261 gcgagatttt tggcctgctt ggccccaacg gcgccggaaa aacaacgacc ctctccatgc
     1321 tctcgaccat gctgaaaccc acatcaggtt ctgcgacaat caacggcatt gatatagaac
     1381 gtgacgagga cggggtcagg aaatccatag gcattgtctt ccaggaccag agtcttgatg
     1441 aagaactgac cgcatgggaa aacatggact ttcacgggcg gctctacagg atccccgccg
     1501 atatacggaa acagcggatc accgagctcc tcaggctggt tgagctggaa gaccggaaag
     1561 atgatattgt gaaaacattt tccggaggga tgaggagacg cctcgagatc gcccgcggtc
     1621 ttctccacca tccttcggtg ctcttcttag acgaaccaac attgggcctt gacccccaga
     1681 cccgcaatca tctctggcag tatattgcca cccttgcaaa agagaagggg atcaccatta
     1741 tcctcaccac gcattacatg gaagaagccg accggttgtg caaccgggtc gccattatcg
     1801 atcacgggaa gatcattgct ctcgacaccc cgcaaaacct caaggacggg attggcgggg
     1861 acgtggtgac catccgatca cccgacccgt cagctatagt cgccgccctg gccgagccct
     1921 ggatcacccg cgttgaacag cataacgatg aagtgattgt cagcctgcag aacgccgaac
     1981 agcacatcag caccatcgtg acgcttctta acaaaagaca ggtcccgatc cagtccatag
     2041 caatccacaa accgacgctt gaggatgtat tcctctcgtt taccggaaaa accatccggg
     2101 aagaagaggc cgaccataaa acgaacatgc ggatgtacca gagaatggtg aggcattaat
     2161 catggatatc atctacacca tctggctcag gaacatgaaa cgctacatac ggtccaaaag
     2221 ccgtatcatc ggcagtatca gcatgccgct cttcttttta ctctttttgg ggttcggcct
     2281 gaattccgtt gtgcagttgc ccggcatggg agggaactac attgtgttcc tgatcccggg
     2341 catggttgca atgagcgtgc tgttcacctc tgttttttcc gggatccaga tcatctggga
     2401 caagcagttc ggctttttga aagagaccct cgttgcgccg gtctcacgcc ttgagatcat
     2461 gttaggccag actcttggcg gggcaacaac cgccgtcctc cagggattca tgatccttgt
     2521 cctctcggtc ttcatcgggc taaagatttc caacccggct ggttttctta ttgctctcgg
     2581 gttcatgatc cttatcggta tatccttcac cgcgttcggc attgcgattg catcacggat
     2641 ggaagatatg accggtttcc agctcatcat gaattttgtt gtcttcccga tattcggcct
     2701 gtccggggcg ctctttccta tcagttccct gccgtcgtgg atgggaccgg ttaccctgct
     2761 cgatccgttg acctacggtg tggaagggat ccgttacggt cttactggcg tatcgcagat
     2821 taatccgctg gtctgtgctg cggttatcgg gggttttacc gttgtgatga cggtccttgg
     2881 ggcattcctg ttcaggaaaa tcaagttctg ataacaataa tcccaccctc tttaagatcc
     2941 agaatagtgg aggaatcatt cttctcacga tcaattgttt tcttagagcg gtttttgggg
     3001 tgggagggga attcgcgttc gtttctttaa aaaaaacaaa agagggactc caaagaaatg
     3061 ttcaaaaacc tgaacgctca acagggattt caatcatacc gcttcgaccc cccgaatgcg
     3121 attaccttga accggtatta catcattacc gcaaaagcga ggggcggtaa gggatgatat
     3181 acctgatccc catcttgttc ttacctgtgt ttttttattt ttcgtgtcct gatacgggga
     3241 actctaccga ccaaaaaagc gtgagcccct gatgggaatc ataataatct aaccgcatat
     3301 atcgcccaaa ttcccgtttt gaaacagata actgcgaatt tttttgtagt caaaggcttt
     3361 tttattactt ggtgcatcac atgtatctgc atgcaaccca agaaaaagac caaaaaacac
     3421 cctctgcttc ccgttctagc ggggtccatg ctcctcatcc ctttcctcct tgctgccggg
     3481 tgtaccgggc aggtaccagc agattcccgg ctgaatggaa ccggatggac cctgacgtgg
     3541 tatgcccaga atggcactct gtcccagtcc cttgccttga caaaagtcac ccttgatttc
     3601 gttaactacg gtcagatcac cggttcggcc gggtgcaacc actactttgc ctcctataaa
     3661 gtggatggta cagcgatcac ctttggtcag gccgggtcaa cggagatgta ttgcggtacg
     3721 ccgggcgtga tggaccagga gagtgcctac ctcacacttc tcggccaggc aaagacggta
     3781 actgttgaag gcgaccgtct cagctttgca gatgccaaag gcacaacgat cctcacgttt
     3841 ataaaaacgg tccctccggc acaggaaccc cttgtcggga caaactggac acttgaatcc
     3901 ttccatacgg cagatgcagt ctcctcggtc ctcgccggta caaccatcac ggctgtcttt
     3961 ggtgccgacg ggagtgtatc cgggtcagcc gggtgcaacc gctactttgc gtcctacaac
     4021 ataaccggaa cttccctttc aattggcccg gccgggtcaa cgaagatggc ctgcggtgca
     4081 ccgggtgtga tgcagcagga aggtaaatac ctgacgctca tcagccaggc aaagaccgta
     4141 attatcaaag gtggcagtct cacgttggca gatgcgaagg gtgcagcaat cctctcgtat
     4201 gcaaaggaag cctgacgggg cagaatgggt caaatccctt tttttaatct tcccccattt
     4261 gtccctcccg tatccgtcca acgggttccg gcccggcagc ccgggcgaca cctcgttatt
     4321 ggtccaggat tcctgccccc ggtcagcgag ccttattctc ctgcacaccc gggtgcttct
     4381 gcaggtgcat tgccatatcg gtagcggtct tgaggagcag gaagagcacc aataccggca
     4441 gggtagaagt aatgccgatg atcccaagca caatgatcac catcgccccg aacatgatgg
     4501 taaagtgcat cgggataatc cggtaatagg gagcggtaaa agtctccgtc ataaaatcct
     4561 tatcctgccg aaggcggttg cggtaataca ggtaggaaaa caggtggttt acaaaaaaga
     4621 gcccgcagga ataccagatg cccgggctgg agagattcac caccccgaag agcccggatt
     4681 ctacgataaa aaagtaatat cctgcatgga atattccgta atgaacagaa aagaatgctc
     4741 caaggatcac ggcctgcgtc cggatactgc cgggagtgac cgtgccgctc tcccctctct
     4801 ctgcacggga gtaattcatt gctgcactga tggaccttgt atcggcgcag aggatcgtaa
     4861 cgacggtgaa gataccaatg atgatactct gggcccagta gatgaatatg actgttgcag
     4921 catcccagtt accggctatc gccagaacga tggtgataag gttggcagaa aggagcgaga
     4981 taaccggtgg ggtaaggtca gacttttccg ggagaatacg atataagggt tgggggaact
     5041 tcataccgta cagcagtgtt gcatcggctg cctaaaggat gttgtgaata tcggaaaatg
     5101 cagatgattc atggggctcg ttttttttgg tacaagagca atgccaacgg aaaacagtaa
     5161 taacggtgcc tgttcgcggc ccccatggaa tggtgtgcag accctcgcac ttgcggtatg
     5221 aaaataagcg aaacgcatat taccttgaaa cgatatctca caataaccca gaaaagggtt
     5281 cgatcacagg agttgaataa aaatgaagga tacccatgaa aaagaacata tatccgttgc
     5341 caaggtcgta gagttgattg gcagctcacc aaacagctgg gaagaagcaa ccgcaaacgc
     5401 cgtcgaggcc gcaggccaga ccatccggaa tatcaagggt gttgatgtaa agcgatgcac
     5461 ggcaaaagta gtgaacaaca agattgtaga atatcgggct gttgttaaaa tcgcgttcat
     5521 cgtggaaagg gaataatctt ttttttttat tttttttatt caaaggtatg gtcatccctt
     5581 attcccggga ttccggagat tttatatggg aaaaggctgc attctcgcat atgttccgca
     5641 tgtgtccata ctaagaaaag gatacattgc acggacttcc tgacatgagg gagaaaacca
     5701 gatgtttcca atcggtgatg ataatacctc ccgcagacta caaccggtgg tcacgtacgc
     5761 cctgatcgcc ctgaacgtcc tgttcttctt gatagagtta atgggaggag atacttttat
     5821 agagacctgg gcgtttgtcc ccagccgctt cctcgcaaat cctggcgggg attttctgac
     5881 gatatttacg tcaatgttca tgcacgccgg ctggcttcat at
//
LOCUS       DASRLV010000006         6873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_733615,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000006 DASRLV010000000
VERSION     DASRLV010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 6873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6873
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733615"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..339)
                     /locus_tag="VFC43_00215"
     CDS             complement(97..339)
                     /locus_tag="VFC43_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29648.1"
                     /translation="MNTVLIFVFKVIFVITLIIGPVSEVRPPARPDLTRHRCPDFFRT
                     YEALRSAEYKTMGFNRAMTVYAVPGAPCIHKHTLLP"
     gene            686..832
                     /locus_tag="VFC43_00220"
     CDS             686..832
                     /locus_tag="VFC43_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29649.1"
                     /translation="MDKQQQPPHVKHDRPICEMCQAANIRTRPDGSYSCNHCGYDSTK
                     KRKK"
     gene            855..1157
                     /locus_tag="VFC43_00225"
     CDS             855..1157
                     /locus_tag="VFC43_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29650.1"
                     /translation="MITIYCLEDCPRCEEIKAKLTTAGYEYSEEDMSTAENITELRVN
                     GCFAMEAPVLRVGDNFYEYCQCQADGFFSEMFGVKQDNQTVADVGGKHWTLKQGEP"
     gene            1373..1693
                     /locus_tag="VFC43_00230"
     CDS             1373..1693
                     /locus_tag="VFC43_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29651.1"
                     /translation="MDEPLTPDEDVVFLEFLRLDVEPNGIPVELYAGFLEQLAASCNM
                     SVQRARVATQELIDKGKFDVVEEKKDDRIQTDREIIIDLLLHDPEVRELLKKIVAEIS
                     PDKT"
     gene            complement(1820..3202)
                     /locus_tag="VFC43_00235"
     CDS             complement(1820..3202)
                     /locus_tag="VFC43_00235"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK29652.1"
                     /translation="MKEYDICVIGAGSAGLVAASAANRSGAKTALIETDRFGGECLHS
                     GCIPSKTFLHSANLYYTMKKAGQYGLPVCQAGTPDLGKVMGHVRDVIGSITLYENAEA
                     YQKLGIDVYFGKNHFVSTNILSVNGNELFSKYFIICTGSSAQVPPVDGLKDIPYLTNK
                     NFWDQTTLPGRALILGAGPIGIELGQALQRLGTEVWITMRSDRILQKEDPAMAEEMKK
                     ILQGDGVQFLDNTTVLGFKKTDSRITARYTKNGSENELVVDAVIVATGRKPNTGDLKL
                     ENAGVAYGKEGILVNDELKTTADNIYACGDVIGKYLFTKAASFYAGIAVHNIVNSGKK
                     TISDSVMPWVIFTDPELAHVGLTEDEAREKFEEVHVLHLDTVFGRLRTENSTKVFLKI
                     ILTGDDRIVGAHALGSGSGEYIQNLTLAMQDHITVKQIAGTIYPYPTFSEIVKKAFSR
                     YLRTKHTTNA"
     gene            3703..4362
                     /locus_tag="VFC43_00240"
     CDS             3703..4362
                     /locus_tag="VFC43_00240"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZK29653.1"
                     /translation="MAKANIPEKGAIVQRDLETFLIHPHIPGGFVEPALLRKIADVAE
                     KYGAKYVKLTGAQRIAIIGLREEDLDNAWAEFEDRSKAIGLTIRSIQICPGTRSCKKA
                     KQDSPGLGFALDKEFYGKPAPAKFKMGISGCTNCCSDSWMKDLGFFGTDDGFTAVAGG
                     KGGGTARLGRQFASGLNEKQAIALARKVIAFYRENAKPLERLGETIERVGFDTFVKTV
                     T"
     gene            complement(4649..6223)
                     /locus_tag="VFC43_00245"
     CDS             complement(4649..6223)
                     /locus_tag="VFC43_00245"
                     /inference="COORDINATES: protein motif:HMM:NF014510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HZK29654.1"
                     /translation="MRRVRDTHYGVRLIVAVLCILLLVTPGVVFAATDGTPSPGTKVT
                     AGPGANGTEVQATPVPVFLSTVNTDSIVENITTIPTTPVPPVYIFLQNATDAVNPPPL
                     LIFDTPVIEDLTCTIYGIAEPGSGNTTIMHILWDWGEDETPEYHEFPNSHTYSSPGVY
                     TLSIIVRQSDGQIRIGTTKVIAGQPVIPVTVPVTPTITVPGGPVMVIHPPVLTLFEPT
                     VDRMNVTLNGNLNAGSPGATIVSLHVDWNDGTSITTDDLPVTHQYSSPGTFTVTITGN
                     QSDGQSATKKIALELRGEMPAPPGPTPSGPAQNTLVPFLSIIAIAITGIVIVAVVLIT
                     RQRKDQPLAKDPHKVFSARTTPGSENRPPLEELTTISSGTGVEPQVLDTVIKVAAEIA
                     REGREGQAIGTAFVVGDTKNVLKCSKQFVLNPFYGHQEAERQITDTGIQGTIKEFAQL
                     DGAFLITGNGVVEAAGRCITVDLSQVDLPGGMGSRHLSVAGITKVTRSIGIVVSQSGG
                     QISIFRNGKIVYVIRP"
     gene            6576..>6873
                     /locus_tag="VFC43_00250"
     CDS             6576..>6873
                     /locus_tag="VFC43_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HZK29655.1"
                     /translation="MEKTGMFQYSTLPEEKLMEHFATSLPDGLSSQQGEERIATSGYN
                     EITASPVHWYEIAIRQFKSAFIYLLLVAAAIVFAVGEYFDAGIILGFVLVNAALG"
BASE COUNT         1688 a         1637 c         1808 g         1740 t
ORIGIN      
        1 ggtttgttct catcacaagg tctttttggg tcatttcgca gcccgaatca agtatttcga
       61 gaactttttt cgaggatttg ggtaaggttg tcgcagtcat ggcagaaggg tgtgcttatg
      121 gatgcatgga gcaccgggca ctgcatacac agtcattgcc cggttaaatc ccattgtttt
      181 atactccgct gaacgaaggg cttcatacgt cctgaagaag tccgggcaac gatggcgggt
      241 gaggtcagga cgggcagggg gccggacttc gctcaccggc ccgatgatta acgtgatgac
      301 gaaaatcacc ttaaagacaa agatcagtac cgtgttcatt gttgcactat ccacccttta
      361 acagtcaaga cattcatgca acccggacca gataagtatt atcctgaaaa tcagcaaatg
      421 ttataaaaaa tgaaatgaaa accctgttgc cagggaatga gacggttttt aataaaatca
      481 tgtgccggtt actgtttttt aagagcttct tgtacaaaga gaactgaacg cattgtctgc
      541 aaaggatgaa caattcattc gtccgtattc ttcaatactt caatggttaa ccgagatggt
      601 gttaaaaaaa aggggacctg cacattattc aacatcccgc ttttaccatg ggcatctttt
      661 tatgggaaca tggcaatcaa tccccatgga taagcaacaa caacccccgc atgtgaaaca
      721 cgatcgcccc atatgtgaga tgtgccaggc cgcaaatatc aggaccagac cagacgggag
      781 ttattcgtgc aaccactgcg ggtatgacag cacaaagaaa aggaagaagt gaagggcttt
      841 ttaagaggga ggcgatgatc accatctatt gtcttgaaga ttgcccgcgg tgcgaggaga
      901 taaaggccaa gctgaccact gccgggtatg agtacagtga agaggatatg tctactgccg
      961 agaacatcac ggagttgcgc gttaacgggt gttttgcgat ggaggccccg gtactgagag
     1021 ttggcgacaa tttctatgaa tactgccagt gccaggcaga cggtttcttc tcagagatgt
     1081 ttggggtgaa gcaggacaac cagacggttg cggatgttgg cgggaagcat tggaccttaa
     1141 agcagggtga gccctgagcg gacagcagca ggtacttccg ggacaatacc ggagagtgcc
     1201 tcatatgcgt aaaaaaactg cacgggcggt tccggaggtg agggtttttg tatcctcagg
     1261 acgagaagat cgtgctcttt cttttttgta ccctgatagc aatttttcaa caagggtctg
     1321 agggtcgata tactgctata tttatggttt atggcaaaaa gaaatctaaa ccatggacga
     1381 acctttaact ccggatgaag acgtggtctt tttagaattt ttacgtcttg atgtagaacc
     1441 gaacgggata ccggtcgaat tatatgcagg gtttttagaa cagctggccg cgtcgtgcaa
     1501 catgtccgtc cagcgtgcac gcgttgcaac acaggaatta attgataaag gaaaatttga
     1561 tgtggtggag gagaaaaaag acgaccggat tcagactgac cgtgaaataa ttatcgatct
     1621 gcttttacat gacccggagg tacgggagct gttgaagaaa atcgtggctg aaatatctcc
     1681 ggacaagaca taagggtagt acaactgcag acgagaagat tcaggtttgg tttgtcgtac
     1741 actcgtcctt tgagtcccgc atttcaggcg gcggaaattt caggaattcc cccggtgtag
     1801 tttttgttga ggggccaaat tatgcattgg tagtatgttt tgtcctcagg tatctcgaaa
     1861 atgccttttt tacaatctct gagaaggtcg ggtagggata tattgtcccg gcaatctgct
     1921 ttaccgtgat atggtcctgc atcgcgaggg tcaggttctg gatgtattct cctgaacctg
     1981 acccgagtgc atgtgcaccg acaatacggt catctccggt aagaataatt ttaagaaaaa
     2041 ccttggtcga attttcagtc ctcaaacgac caaacacggt atcaaggtgg aggacatgga
     2101 cctcttcgaa tttctcccgc gcctcatctt ctgttaaacc aacatgggca agttccggat
     2161 cggtaaagat aacccagggc atgacactgt cagaaattgt ctttttcccg ctattgacaa
     2221 tgttatggac cgcaatcccg gcataaaatg atgctgcctt cgtgaacaga tattttccga
     2281 ttacgtcgcc acatgcataa atattatcgg cggttgtctt cagctcatcg ttgacaagga
     2341 ttccttcttt tccatatgca acacctgcat tttcaagctt caggtcaccc gtgtttggct
     2401 tcctgccggt ggctacgatg acagcatcca caaccagttc attttccgat ccgttttttg
     2461 tatagcgggc agtgatacgg gaatctgtct ttttaaatcc cagcacggtg gtattgtcaa
     2521 ggaattgcac cccgtccccc tggaggattt ttttcatttc ttcggccatc gcgggatcct
     2581 ctttttgcag tatcctgtcc gaccgcatag taatccagac ttcagtaccc agtcgctgga
     2641 gtgcctgtcc aagttcgatc ccgatcggac cggcaccaag gataagggcc cttccgggga
     2701 gcgttgtctg gtcccagaaa tttttattgg taaggtaagg gatgtctttt agtccatcca
     2761 cgggaggaac ctgtgcagat gagccggtac agatgatgaa atacttcgaa aacagttcat
     2821 tgccgttgac ggacaggata tttgttgaca cgaaatgatt ttttccaaaa tagacatcaa
     2881 tacccagttt ctgataggct tctgcgttct catagagcgt gatagaaccg ataacatccc
     2941 gtacatggcc catcaccttt cctaggtccg gcgtgccggc ctgacagacc gggagcccgt
     3001 actgcccggc cttcttcata gtataataca ggtttgcaga atggaggaac gtcttactcg
     3061 ggatacatcc tgaatggaga cattcaccgc caaaccggtc tgtttcaatc agcgcagttt
     3121 tggcacccga tcggtttgcc gctgaagctg caacaagacc ggctgaacct gcacctataa
     3181 cacaaatgtc atactccttc atgctttgtt cctgctaaac tgatgttcac acttaccctg
     3241 aataacgaat atattcctta tttccacgag tgaatttttt gacatgagcg atttcctgat
     3301 gaattgcggc ctgtgcttcc tcgcaggtag tatcgggaga cgtaccaacc tttgctttga
     3361 aatgtcagct ggtgtgtttt tttctcaggc gaaccgtatt acgaccgtaa gtccataaat
     3421 atcttaccgg tcgttgcaga atctgaaaca acagggcata tccggtgttt tttttaatat
     3481 tttacaggac tatggagaat gaagacaaaa aaaagcatgt gttccggcgt accagtctga
     3541 ccggtaactt cgtcccggat caattttttt tcccaagttt aagttccagg ataatcagga
     3601 gagtccattt tttgttgcat atgtatgtct tgtcactttg catggttatt tatcatttta
     3661 ttgataagga aaaagcgtat caaattttat taaggaggaa tcatggcaaa ggcaaacata
     3721 cctgaaaaag gagcaatcgt tcaaagagac ctggaaacgt ttttgatcca tccccatata
     3781 cctggtggct tcgtcgagcc tgcactgttg aggaagatcg ctgacgtagc agagaaatac
     3841 ggggcaaaat acgtgaagct cactggtgcc cagcgcatag ccattattgg tttacgtgaa
     3901 gaggatctgg acaatgcatg ggcagagttt gaggaccgtt ccaaggccat tggcctgacg
     3961 atacggagca tccagatatg ccccgggacc cgatcatgta agaaagccaa acaggacagc
     4021 cccggcctgg gattcgctct tgacaaagag ttttacggca agccggcacc agccaagttc
     4081 aagatgggca tatcaggttg caccaactgt tgcagtgact cctggatgaa agacctcgga
     4141 ttcttcggca cagatgatgg gttcacggca gtcgccggcg gtaaaggtgg agggaccgcg
     4201 aggcttggcc ggcagttcgc cagcggactg aatgaaaaac aggctatagc cctcgcaagg
     4261 aaggtcatcg ccttttaccg ggagaacgcc aagcccctcg aacgtctggg cgaaactatc
     4321 gaacgagtcg gtttcgacac atttgtaaaa acagtgacct gatattcctg tccagcatat
     4381 ctgccacggg gataacgatt ccgtgccgtg gatgatggct catatttcca gtcagcatac
     4441 ggttggatca tcacgctgcc gtggattatc aacacacccc tttttaacga tgagcaggct
     4501 gatgatcaag acggggaatc tttccgggca ggcatcctgg taaccagtgt gatcaggagc
     4561 agtaattctc ctgatagtcc tcttacctcg ttcgctggat aaggatcgga aaatatcttt
     4621 ttggggagtt cagcactggc tgcttttctc acggcctgat aacgtacacg atcttcccgt
     4681 ttcggaaaat ggagatctgg ccaccgctct gcgagacaac aatcccgatc gagcgggtaa
     4741 ctttggtgat gccggcgacc gacaggtgcc gggaccccat ccccccgggg aggtccacct
     4801 gactcaggtc aaccgtgatg cacctgcctg ctgcctcgac cacgccattg cccgtgatga
     4861 ggaacgcccc gtccagctgg gcaaactcct tgatcgttcc ctggatcccg gtatcggtga
     4921 tctgccgttc ggcctcctgg tggccataga acgggttcaa tacaaactgc ttggaacatt
     4981 tcaggacgtt ttttgtgtcc ccgacgacaa aggccgtccc gatagcctgt ccctcccgcc
     5041 cttcccgggc aatttctgca gcaaccttga tgaccgtgtc caggacctgg ggctcaacac
     5101 ccgtcccgga agagatcgtt gtcagttctt ctaagggagg gcggttttct gacccggggg
     5161 ttgttctcgc cgaaaatacc ttatggggat cttttgccag gggctgatcc tttctctgcc
     5221 gggtgatcag gacgactgct acgataacaa taccggtgat ggcaatggcg atgatgctta
     5281 agaatggcac cagggtattc tgggcaggac ctgacggggt cggcccggga ggagcaggca
     5341 tctcgcccct gagctccagc gcaattttct tggtggctga ctggccgtct gactggtttc
     5401 ccgtaatggt cacggtgaat gtccctgggc tggagtactg gtgggtcacc gggaggtcgt
     5461 cggtggttat gctggtcccg tcattccagt cgacatgtaa agagacgatg gtggcgcccg
     5521 gcgatccggc attgaggttc ccgttcagcg taacattcat ccggtcaacc gtcggctcaa
     5581 agagggtcag gaccggcggg tgtatcacca tgaccggtcc ccccggcacg gttatcgttg
     5641 gcgttacggg gaccgtaaca gggatgaccg gctggcccgc gatgaccttt gtggtcccga
     5701 tcctgatctg tccgtcagac tgccggacga tgatcgacag cgtataaaca ccgggactac
     5761 tgtaggtatg cgagttgggg aactcatggt actccggcgt ctcgtcctct ccccagtccc
     5821 agaggatgtg catgatagtg gtgtttcccg acccgggctc agcaatcccg tatatggtgc
     5881 aggtcaggtc ttcgatgacc ggtgtatcga agatgaggag cgggggaggg ttgaccgcat
     5941 cggtcgcatt ctgcaggaag atgtacaccg ggggaaccgg tgtggtcgga atggttgtga
     6001 tgttttcgac tatgctgtct gtattcacag ttgagagaaa cacggggacc ggtgttgcct
     6061 gtacttcagt accgttcgcc ccgggtccgg ccgtgacttt tgtaccaggg ctgggggtac
     6121 cgtctgtcgc agcaaagaca acaccgggcg ttacgagaag gaggatgcat aggacagcga
     6181 cgataagcct taccccgtaa tgtgtgtctc gtaccctgcg catggaaaat ccgttctttt
     6241 gctctataaa tatcgctatg attgctctta aatacggttg ttttccttgc gattgtgtac
     6301 gtttcggaat atcaccacgg gaataacgaa agtccctttt caggctcttt acctccacag
     6361 aatttgatcc cgttaccgga cataaccccg cgatacgagt ccatacttcc acctgatcca
     6421 ggggggcaga gaatcgcgat actatagata acccggtacc attgaaactg tcatgaagga
     6481 gtcgtgagaa aaaagaaggt ggacaaagcc ggcaggtgcc ggatgcaata atatcttata
     6541 aggattgaaa tcgatagtgt cggtaaagga tgaacatgga aaagacggga atgtttcagt
     6601 actcaacact ccctgaagag aagctaatgg agcatttcgc tacctcgctt ccggacgggc
     6661 tatcatcgca gcagggagag gagcggatcg caacatcggg atacaatgag atcaccgcga
     6721 gcccggtcca ctggtatgag atcgcgatac ggcagtttaa gtcggccttc atctacctgc
     6781 tcctggttgc cgcagccatt gtctttgcag tgggagaata tttcgacgct ggaattatcc
     6841 tcggttttgt gctcgtcaac gcagcgctcg gct
//
LOCUS       DASRLV010000007         3135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_246091,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000007 DASRLV010000000
VERSION     DASRLV010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246091"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1249)
                     /gene="ligD"
                     /locus_tag="VFC43_00255"
     CDS             complement(<1..1249)
                     /gene="ligD"
                     /locus_tag="VFC43_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HZK29656.1"
                     /translation="MSEYQPMLAKTIHAPFNSDEWFFEIKWDGVRAIASVNDTVSLKS
                     RNNHELSGQFPELNELLHLAPGTVLDGEIVIMSGGKPDIQSLLPRLHKGRGKIPPSQV
                     RVPVTYIVFDILKKDKKPLVSLPLVERREILKQAVKEGPHVVLSVPVTGRGEDYYRAA
                     VARGLEGIMAKRMNGRYEPGLRSDNWLKIKAGRTCDCVIAGYTPGQGGRSPAFGALIL
                     GLYENMSGDATVIAGQSPGKGSGEGGLVHIGNVGSGFSDQDLHDLMGMFSSLKTDTPH
                     FVVREQKGTVVWLEPLLVAEVAYQEVTRDRKLRIPRFIRLRTDKQAEECTTDQLVPVK
                     VDLVKSGQVPDRAPAGIHQRSGDSKGAEKPVLGRSPESPPAHALKDYQKKRDFLKTKE
                     PEGTTIMTGKGNYFVIHEHHARHT"
     gene            complement(1275..2246)
                     /locus_tag="VFC43_00260"
     CDS             complement(1275..2246)
                     /locus_tag="VFC43_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HZK29657.1"
                     /translation="MTPGRQSGDKAVGGSSPKKAIKNKKGDNGPESVLSPEAPAIDAK
                     KDQHGSTVTREHSPDEPEQKGTAGRPFWSGSITIGLVNVPVRLHTMVRDRSFSFRLLH
                     RDDGQPLRYDRVCTKDGRVVPWAETVKGYEVEKGRYVVFEPDELKAIMPDSDRKIRIE
                     KFVYFLSLDPTYFETPYILVPDRSEEAYSLLVTALTELGRAAVGTITLRTKEYPVVVH
                     VYQGGLVLTTLRHADEVTSPHMFEQLTSLPVPKDSELTLAKRIINELSGDFSITDYPD
                     RYQKAVMELIKKKRAGEKIVIEEPPHPEQAKELMQALQETIATLEMK"
     gene            complement(2218..>3135)
                     /gene="ligD"
                     /locus_tag="VFC43_00265"
     CDS             complement(2218..>3135)
                     /gene="ligD"
                     /locus_tag="VFC43_00265"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HZK29658.1"
                     /translation="RNINYFKMTGSAGNNGKELSLGDLTRVRLTNLDKVMYPAISVTK
                     QDVITYYIRMAPRILPFLRNRPLTMHRFPGGVEGEAFYEKDAPQGTPDYVEIFTHPDK
                     TADRDLHYVLCNNLDTLIWLANLASLELHATLSASVSYDSPDLLLFDIDPEPPLTFDD
                     VIDVARIVREHLENAGITSFVKTSGKKGVHIVVPLSKGHRFEEVRAFAHTVGKTIARD
                     TSHVVAEFARSRDPGTIFIDYLQNARGKTMAAPYSLRATAGATVSAPLSWERLRRGIH
                     PEDFNIRTMLFGSEDPWRGIFDTRQTIGR"
BASE COUNT          642 a          860 c          821 g          812 t
ORIGIN      
        1 gtgtgtggcg tgcgtgatgt tcatggataa caaaataatt tccctttcct gtcatgattg
       61 tcgtcccttc cggttctttt gtctttaaaa aatccctctt cttctggtag tctttcaatg
      121 catgtgccgg gggtgattcc ggggaccggc ctagtactgg tttttccgca cccttactat
      181 cgccggatct ttgatgaatg cctgcaggtg ctcgatccgg gacctggcct gacttcacga
      241 gatcaacctt cacgggtaca agctgatcgg ttgtgcactc ttcagcctgc ttatcggtgc
      301 gtaacctgat aaaccgggga attctgagtt tccggtccct tgttacctct tggtatgcca
      361 cttcagcgac cagcagcggt tccagccaga ccacagtacc tttctgctcc cgcactacaa
      421 agtgcggcgt gtcggttttt aaagatgaga acatccccat gagatcgtga agatcctggt
      481 ccgagaaacc tgaaccaaca ttgccgatat gcacaagtcc cccctctccc gatcccttcc
      541 cgggtgactg gcctgcgatg acggtcgcgt ccccgctcat gttttcgtac agcccgagga
      601 taagtgcccc gaatgcaggg ctgcgccctc cttgtccggg cgtgtaaccg gcaatcacgc
      661 agtcacaggt cctgcctgcc ttgatcttca gccagttgtc gctgcgaagg ccgggctcat
      721 agcggccgtt catccgcttt gccatgatcc cttccagtcc ccgtgcaact gctgcacggt
      781 aatagtcctc acctcttccg gtcaccggga cggagagtac aacatgggga ccctccttaa
      841 cggcctgttt caggatttcc ctccgttcaa ccagcgggag tgatacgagc ggttttttat
      901 ctttttttaa gatatcgaac acgatgtagg tgacaggaac gcgaacttgt gaagggggta
      961 tttttccgcg tcccttgtgc aaccggggaa ggagcgattg tatatcgggt ttccccccac
     1021 tcataataac aatttccccg tcgagaactg tgcccggggc aaggtgcagg agttcgttga
     1081 gttcggggaa ctggccgctc agctcatggt tgttccggct cttcaaagat actgtgtcat
     1141 ttaccgatgc tatagcccgg acaccatccc acttgatctc aaaaaaccac tcatcgctgt
     1201 tgaagggagc atgaatggtt tttgcgagca tgggctggta ttctgacatt atatcccctc
     1261 aaaaatgccg gtattcattt catctcaagc gtggcaatgg tctcttgtaa tgcctgcatg
     1321 agttcttttg cctgctcggg atggggcggt tcttctataa caatcttctc accggcacgt
     1381 ttctttttga taagttccat gacggctttt tggtaccggt caggataatc cgtaatcgaa
     1441 aaatcacctg aaagctcatt gattatccgt tttgccagcg tcagttcgga atcttttggc
     1501 acggggaggc tggtcagttg ttcaaacata tggggggatg tgacctcgtc tgcatggcgg
     1561 agtgttgtca ggacaagacc tccctggtag acgtgcacca ccaccgggta ttcctttgtc
     1621 ctgagggtga tcgtcccgac tgccgcccgt ccgagttcag taagtgcagt aaccagcaga
     1681 ctgtaggcct cctcgctccg gtccggaaca aggatgtacg gagtctcgaa ataggtcggg
     1741 tcgagcgaga gaaaatacac gaatttctca atccttatct tccggtcgga gtcgggcatg
     1801 atggccttga gctcatcggg ttcaaagacc acgtacctgc ccttttccac ctcgtaccct
     1861 ttcacggttt cagcccacgg gacaacccgt ccgtcctttg tgcagacccg gtcgtaccgg
     1921 agcggctgcc cgtcatcccg gtggagcagc ctgaacgaaa acgaccggtc cctcaccata
     1981 gtatgcaacc tcaccggtac attgaccagc ccgatcgtga tgctcccgct ccagaacggc
     2041 cgtcccgcag tacctttctg ttctggttca tccggtgagt gttcccgtgt gaccgtagat
     2101 ccgtgctggt ccttttttgc atcaattgcc ggtgcttcag gagaaaggac agattctggt
     2161 ccgttatctc cttttttgtt ctttatggcc tttttcggtg atgaaccgcc gacggcctta
     2221 tctcccgatt gtctgcctgg tgtcaaagat acctctccat gggtcttcac ttccaaaaag
     2281 cattgtccgt atattaaaat cttcgggatg tatcccgcgc ctgagtctct cccagctgag
     2341 cggggcagaa accgttgcac cggcagtagc ccggaggctg tacggtgcag ccatggtctt
     2401 tccccgggca ttctgaagat agtcgatgaa gatagtcccg gggtcccgtg accgggcaaa
     2461 ctctgcaacc acgtgggaag tgtcccgtgc aatcgttttt cctaccgtat gggcaaatgc
     2521 ccgcacctcc tcaaaccggt ggcctttgga aagcggcacc acgatatgga cacctttttt
     2581 gcccgaggtt tttacaaacg acgtgatccc ggcattttcc aggtgctcac ggacaatccg
     2641 ggcaacatcg atcacatcgt caaaggttag cggaggttcg ggatcgatat cgaacagcag
     2701 gaggtccggg gaatcgtacg agaccgatgc agaaagcgtg gcatggagtt cgagggatgc
     2761 aaggtttgcc agccagatca gcgtatcgag gttgttgcac agcacgtaat ggagatcgcg
     2821 gtccgcagtc ttgtccggat gggtaaagat ctcaacgtaa tcaggtgtgc cctgcggggc
     2881 atctttctcg taaaaagcct caccttccac accacccgga aacctgtgca tggtgagggg
     2941 ccggttacgg agaaagggaa gaatacgggg cgccatcctg atataatagg tgatcacgtc
     3001 ctgctttgtc acagagatgg ccgggtacat cactttgtcg agatttgtga ggcggactct
     3061 cgtgaggtca ccaagggaca gttccttgcc gttatttcct gcactcccgg tcattttgaa
     3121 gtaattgatg ttcct
//
LOCUS       DASRLV010000008         5612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_489753,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000008 DASRLV010000000
VERSION     DASRLV010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489753"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..674
                     /locus_tag="VFC43_00270"
     CDS             198..674
                     /locus_tag="VFC43_00270"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZK29659.1"
                     /translation="MQKITPFLWFDTHAQEAMDFYVSVFENSKVISVTRYGEGGPGPK
                     GTVMTAKIHLDGQEFVLLNGGPQFSFSQAISFVVNCASQDEVDWFWEKLSEGGDKQSC
                     GWLKDKFGVSWQVVPVVLQEMLTGADVQKSQRVMMALLQMGKIDIQKLKQAYNNAG"
     gene            776..1336
                     /locus_tag="VFC43_00275"
     CDS             776..1336
                     /locus_tag="VFC43_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZK29660.1"
                     /translation="MANVILGVTISLDGFAEDRNGSVGTLYPDLEIYDTTDVMREAVR
                     DTGAVLMAWKEFAMAEDPDLFAGNYEFQVPIFVVTDKSPKKHPKETDRLTFTFVTDGI
                     ENAVRQAKAAAGDKNVTIIGSAFTTRECLKEGLVDELHIDIISVFLKAGFRPFEDNGT
                     TSIRVERIKVVELPAGRTHLRFRVVK"
     gene            1448..1912
                     /locus_tag="VFC43_00280"
     CDS             1448..1912
                     /locus_tag="VFC43_00280"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HZK29661.1"
                     /translation="MKHTTFIYLGIICIIAIACAGCTGPGPVNPVDSTPAPSPAKTAI
                     PVGNIVVDEEQNGATTSMDLDRTITLRLKENPSAGYVWDLTVTPGLQVTNDIFYPSDT
                     SGSLVGAGGTRVWTITTLHSGEQKVKAIYKHPWEPVTGKETMFNLTIMVSPL"
     gene            complement(2046..3899)
                     /locus_tag="VFC43_00285"
     CDS             complement(2046..3899)
                     /locus_tag="VFC43_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK29662.1"
                     /translation="MSVLWKYLKPQRGLIVVALVLAGIGQLLSLVDPLIFGKIIDDYA
                     LNPSNSPEEALIRGVLFWLGVAVSVAVLAQVAKAFQDYFTRLAVKKFGMQIFNDGLKQ
                     TLRLSYDEFEEQRSGETLSILQKVRDDTQIFMNSFINVVFSSVVGMSFLIGYSLTRNW
                     LLIPVFVIGIAVLGSLTGLLSKKIKMTQRSINRETYKISGVITESLRNIELVKSLGLT
                     FPEIRRLQDQNLKIFNLEMMKVKRIRLLTFLQGATLSILKQSILFILLWLIFRKALTT
                     GELISMQFISTIIFGPLQDLGSIIISYHEVESSVNNFDQLMQKPIEQNVENPIEIDEL
                     SDIRFENVVFRHKSAHYNAVDGISFEVKTGETIAFVGPSGAGKSTLVKLLLGLYSPVS
                     GEIWFNGHPSSQIRYNPLRRQIGFVSQDAQLFAGTIKENLLFVQPDATGEQMMDALHK
                     AACDNLLSNSAHSIDTIIGESGMNLSGGEKQRISIARALLRHPRLLIFDEATSALDSL
                     TEEDITNTIRDISTSRDQISLLIAHRLSTIMHADVIYVLEQGKIVETGSHESLLDQKG
                     LYYAMWRQQVGERRSAVTSPVSQSAGISPRKTGNDGISDSEFAHEKDEHSH"
     gene            4208..4507
                     /locus_tag="VFC43_00290"
     CDS             4208..4507
                     /locus_tag="VFC43_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29663.1"
                     /translation="MGHIRTDSSTRYHCASLICDYFLSKNITHADVSARDIAKYHNLT
                     GKTCRSLSAFLNYLYSNRIRKVGFGFYILGTLAFSKSDYPHRFTIELIDGARGLL"
     gene            4663..5037
                     /locus_tag="VFC43_00295"
     CDS             4663..5037
                     /locus_tag="VFC43_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29664.1"
                     /translation="MTQEEGKDYEHRRRNRHLIPAVLFIGLGVGLLLNYPGPGLLIGM
                     GLGFIGSTLMTPAVYPSGDAAETGRHRGSGHRIIMALLGIFFVILGISLIVAPPNFWP
                     YFGAGFLILLGIWFIARSLGKA"
     gene            complement(5093..>5612)
                     /locus_tag="VFC43_00300"
     CDS             complement(5093..>5612)
                     /locus_tag="VFC43_00300"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HZK29665.1"
                     /translation="VQTTPLMGIVFQDAANTAVYVAQNKTGVSLAGSDVIFSIEAEHQ
                     ISSVDGPSAGALMTLVMISALKNQPLDPGVTLTGTIDKDGHVGAIGGVIEKATAAQEN
                     KKILFLLPKENSELVQYTTKRTSYGGLVLVERVPERISAQDYIRKNIGINVTIVDSID
                     DVIAAGPKTVLI"
BASE COUNT         1459 a         1406 c         1324 g         1423 t
ORIGIN      
        1 ggaattgaac caacatcacc tggaccaaat gcttcttctt tccccgcctc ttttgcatct
       61 ttagaggcaa ccgtcacact ggtcaaagag atcatcaagg cgagaatgac agaaaatgaa
      121 acgaaggcaa aaaaataagt accctcctga tcctgtcaca ttaataattc aatagaaagg
      181 aaaagaggta aactggtatg caaaaaatca ccccatttct gtggttcgat acccatgcgc
      241 aggaggcaat ggatttctat gtctcggtct ttgagaactc aaaagtcatc agcgtaaccc
      301 ggtacggcga aggagggccc gggccgaaag gaacggtcat gacggcaaag attcacctgg
      361 acgggcagga atttgtcctc ctgaacggtg gtccccaatt ttccttctcg caggcaatat
      421 cctttgtcgt taactgcgcc tcacaggatg aggtagactg gttctgggag aaattgtctg
      481 aaggtggtga taaacaatcc tgcggctggc tgaaagacaa attcggtgtt tcctggcagg
      541 ttgttccggt tgtcctgcag gaaatgctga ctggtgcgga cgtacaaaaa tcacagcggg
      601 tcatgatggc cctgctccag atgggaaaaa tcgatataca gaagctgaag caggcctaca
      661 acaatgcagg ataatgttcg gtacgtttgc cttttaatag ggatcaacgc aaccggtcgt
      721 tttcttttgg tacacgtgaa aaatattaaa tcgaaaacag ggtaaaacac cagtcatggc
      781 aaacgttatt ctgggggtga caatttcact ggatggattt gccgaagatc gcaacggcag
      841 tgtgggtacc ttgtatcccg atctcgaaat ttacgatacc accgatgtca tgcgggaagc
      901 cgtacgggat accggtgcag tcctgatggc atggaaagaa tttgcaatgg cagaggatcc
      961 ggacttgttt gcgggcaatt atgaatttca ggtcccgata ttcgttgtca ccgacaaatc
     1021 tcccaaaaag catccaaaag agactgacag gttaacgttt acgttcgtca cggatggtat
     1081 cgaaaatgcg gtccggcagg caaaagcggc ggcaggagat aaaaacgtca caattatcgg
     1141 gagcgccttc actactcgtg agtgtttaaa agagggacta gttgatgaac tgcatattga
     1201 catcatatcg gtatttttaa aagccggttt ccggccgttc gaagataatg gtactacatc
     1261 catcagggtg gaaagaataa aagttgtgga actcccggcc ggcaggacac acctgagatt
     1321 ccgggttgtg aaataaacct gctatcgcaa ttatggaaga ttttgagtat tagtctctca
     1381 tttatcctgt tcagagagat aaatgcataa ataattcccg gaacaaacct acagcgaaag
     1441 gcagagcatg aaacacacga cttttattta tcttggtatt atctgcatca tcgccattgc
     1501 atgtgccggc tgcacgggtc ctggcccggt aaacccggta gacagcaccc ccgcgccctc
     1561 tcccgcgaaa accgcaattc cggtcggaaa tattgtggtg gatgaagagc agaatggcgc
     1621 aacaacatcc atggacctgg acaggacgat cacgctcagg ttaaaagaaa acccttcggc
     1681 ggggtacgta tgggacctca ccgtgactcc ggggctgcag gtcaccaatg atatctttta
     1741 tccttctgat accagcggga gcctggtcgg ggctggaggg acacgggtct ggactatcac
     1801 gaccctgcat tcgggagaac agaaggtgaa ggcgatctat aaacacccat gggaaccggt
     1861 caccggcaaa gagaccatgt ttaacctgac catcatggtc agcccgctct aggcttctcc
     1921 acgttatcct ttctttttgt atcctgtctg cgataagagg tcctcaaccg tccatgagaa
     1981 ataccgggtg ataaaataaa cggtaagctc aacaaccatg atttttcccg gagagccttc
     2041 atcatctagt gcgaatgctc atccttttca tgcgcaaact ctgaatcgga aataccgtca
     2101 ttacctgttt tccggggaga aataccggca gactgcgata cggggctggt caccgcactt
     2161 cttcgctcac caacctgctg gcgccacatt gcgtaataca aacccttctg gtcaagaaga
     2221 ctttcatggg aaccggtctc aacaattttg ccctgctcca gtacataaat cacatccgca
     2281 tgcatgatcg tagacagccg atgggcgatc agaagggaga tctggtccct gctcgtggaa
     2341 atatccctga tcgtatttgt tatatcctcc tcggttaacg agtccagtgc cgacgttgct
     2401 tcatcaaaga tcagcaaccg tggatgacgc agcagcgccc gggcaatgga aatacgctgc
     2461 ttctcacctc ccgacaggtt cattccgctc tccccgataa tcgtatcaat actgtgagca
     2521 gagttggaaa gcaggttatc acaggcagcc ttgtgcaatg catccatcat ctgctcgcca
     2581 gtcgcatccg gctggacaaa caggagattt tccttgattg ttcctgcaaa caactgtgca
     2641 tcctgagaaa cgaaaccgat ctgcctccgt aacggattat accggatctg agaagacggg
     2701 tgaccattga accagatctc tccgctcacc ggactgtata atcccaggag gagctttacc
     2761 agcgttgact tgcctgcacc ggaagggccg acaaaagcga tcgtctcacc ggtcttcacc
     2821 tcaaaagaaa ttccatcgac ggcattataa tgcgcagact tatgacggaa gaccacattc
     2881 tcaaaacgga tatcactcag ctcatcaatc tcgatcggat tttccacatt ctgttcaatg
     2941 ggcttttgca tcagctgatc gaaattattg accgatgatt caacctcatg gtagctgata
     3001 ataatactgc caagatcctg caaaggcccg aaaatgatcg tggagatgaa ctgcatcgag
     3061 atcaattctc cggtggtaag ggcttttcgg aaaattaacc agaggagtat gaacaatatt
     3121 gactgcttga ggatgctgag cgttgctccc tgcaggaacg tcaggagacg gatccttttt
     3181 actttcatca tctcaaggtt gaagatcttc aggttctggt cctgtaatct gcgtatctcg
     3241 ggaaatgtca ggccaagact ttttaccagc tcaatattcc ggagggattc ggtgatcacc
     3301 cccgatattt tatacgtttc gcggttgatc gaccgttggg tcatcttgat ctttttactc
     3361 aataatccgg tgagtgaacc cagcacggca ataccgatca caaaaaccgg tatcaacagc
     3421 cagttcctgg taagcgaata ccctatgaga aaactcatgc caacgacaga agagaagacc
     3481 acgttgatga acgaattcat gaatatctgg gtatcgtccc tcaccttctg caggattgat
     3541 aacgtctcac cgctccgttg ttcctcgaac tcatcatagg ataaacggag tgtctgcttc
     3601 aacccgtcat tgaagatctg cataccgaac ttttttactg ccagccgggt gaaataatcc
     3661 tggaaagctt ttgcaacctg ggcaagcact gcgacagaga cagccacacc cagccagaac
     3721 agcactcctc tgatgagtgc ctcctctggg ctgttggacg gatttaaggc ataatcgtca
     3781 ataatcttcc caaaaataag cggatcgaca aggctcagca actgaccgat gcccgcaaga
     3841 acaagagcaa cgacaataag ccccctctgc ggttttaaat acttccaaag gacactcatt
     3901 tttgtgggat aacgacgata tttatggttc tcctgatatt tttttctttg gtgtctgcct
     3961 gctcctttgc cttctttaca cagtacgtgc agggagtaac ttataaccaa tgtatgcagc
     4021 cccgttgaaa aagtatcgcc gggccggttc gcgggccgga tatggttcac tatcgctctg
     4081 caaacatctt tcgttatacc tccaacctcg gaattccggc ctttttcgcg ggatattttc
     4141 ttaaaaaagg cgtgatacgt aactatttcg tcccatacag ctaatactgg tgaaagaagg
     4201 aatggctatg gggcatatac ggacggattc cagtacacga tatcattgtg catccctcat
     4261 ctgcgattat ttcctttcaa aaaatatcac ccatgccgac gtatcagcac gggatatcgc
     4321 aaaataccat aacctgacgg gcaaaacctg ccggtctctc agtgcatttt tgaattattt
     4381 atacagcaac cggatcagga aagtggggtt tgggttttat attttaggta ccctggcttt
     4441 ttccaaaagt gactatcccc accggtttac catcgaactg atcgatggag cacggggttt
     4501 gctctgattt tttgcatttc ctgtccttac cttcaactgc catgccgttt tgtatataaa
     4561 gaataactgc acctgtgcct cgtttcagaa attatcggcg ttttttcctg gctctaaaaa
     4621 aattccatac ctttattact cctgcaggac aacctgcgcc gtatgacaca agaagaggga
     4681 aaagactacg aacaccgtcg ccggaaccgc catcttatac cggccgtcct attcatcggc
     4741 ctcggggtcg ggcttctttt gaattatccc ggccctggcc tgctgattgg catggggctc
     4801 gggttcatcg ggtcgacact gatgaccccc gcagtgtacc cctccggaga tgctgctgaa
     4861 acaggaaggc accgcgggag cgggcaccgt atcatcatgg ccctgctcgg gatctttttt
     4921 gtgatccttg gtatcagcct tatcgtggca cccccgaatt tctggcccta ttttggtgct
     4981 ggtttcctta tccttctggg gatctggttt atagcccgga gcctgggaaa ggcataggaa
     5041 acgggtatcc ctcctcatct tttaccaaag aaaaaagtgg atttcctggt gtttatatca
     5101 ggaccgtttt aggtccggcg gcaatcacat cgtcgattga gtcaacgata gtgacattga
     5161 tcccgatatt cttccggatg tagtcctgtg ccgaaatccg ttccggcacc cgctcaacga
     5221 gtaccaaccc gccataactg gtccttttcg ttgtatactg caccagctca ctgttctctt
     5281 ttgggaggag gaacagtatt tttttgtttt cctgtgccgc agtcgctttt tcgataacgc
     5341 cgccaatcgc accgacatga ccatctttat caatagtacc agtcagcgtt acacccggat
     5401 caagcggctg gtttttcagt gctgagatca tgacaagggt catcagggcg cccgcactgg
     5461 gcccgtcaac tgaagatatc tggtgctccg cttcaatcga aaagatcaca tcgctgccgg
     5521 caaggctgac gccggtctta ttctgtgcta catagactgc ggtgtttgcc gcatcctgga
     5581 acacaatacc catcagcggg gttgtctgga ca
//
LOCUS       DASRLV010000009        13693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1059018,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000009 DASRLV010000000
VERSION     DASRLV010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 13693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13693
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059018"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(264..794)
                     /locus_tag="VFC43_00305"
     CDS             complement(264..794)
                     /locus_tag="VFC43_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29666.1"
                     /translation="MRWAYLLVILLALMGISAMPVAAAPVLTISATPHQAHIGDVVTL
                     NGTVSGITTIAVYLFVTGPDLDTRGVTLDNLNVPAGRGLFTTAPVQMNDGTWTYTWDT
                     SVILGVMSPGKYTIYVVSSPVDRLRYVAGDIATVDVEFLASDKPAAETPLEPVIPVIA
                     VGIAGILGLVMFRMQG"
     gene            complement(846..2147)
                     /gene="brxD"
                     /locus_tag="VFC43_00310"
     CDS             complement(846..2147)
                     /gene="brxD"
                     /locus_tag="VFC43_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding protein BrxD"
                     /protein_id="HZK29667.1"
                     /translation="MNTPVPEQMDRRRLESMNIINALRRGTVPAGGLERIAVGLDIEE
                     GVISKQLDYVAQGGGDTKFIRGEYGSGKTFLVARALEIARTKGFVTSHVIISSSTPLH
                     KLKGIYQQICANLRTSDEEHALKTIIDNWLYSIEERLISVSGTAGEDLALEGATEREI
                     EASLAGISEVNTALAAALRTYYKANNAGEFQLAQSALGWISAEPNIGRDFKQKAGIRG
                     DIDDTIAFIFLRGLVSIIHGAGYRGIAIAVDEMETTQGLQRNQREKGYQTLVQIIDAL
                     DRGEMPRCYFLFTGTPALYDGSRGIRSIPPLYDRINVVQTDAFRNPRQPQIILEKFDT
                     SKLELVGLKVVDVYAQAYSEPDRERVSHRFVRAMIKKITTRFGGRVDVIPRIFLKEFV
                     DVLDKCELYEDYNPADAYDFDTEHLKGELKKEEEAVMVVRF"
     gene            complement(2128..3942)
                     /locus_tag="VFC43_00315"
     CDS             complement(2128..3942)
                     /locus_tag="VFC43_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29668.1"
                     /translation="MIPASIDDRKKEAFRLFEDGRYQESLDVCNALLEGEKDSAVEVL
                     AATNLYYTGRFEDAEVFFRDLSQKMQESSYIHSYLGKVLEARKDEGAITEYALAVHLD
                     PTNQDALRSYAAYLISHKDYRGSLPVLRRLVQLGKKPADVQHLMRVLIEIGEPQEALA
                     ISAILGGDAQKTPEYIDALIKTQNFPAAAELAYRIYRDTQDPQIHRKYLDTLSRYDMQ
                     ASLGAYESHIQKEPDEEILFDYILLLQAKGDYAPALAALKPLLDRAGDPLYQLVGCDL
                     LAASGDASKALESYENLIRYELGSKNDLDLLARIIDKYRRYLKAHVPTEEARKRFLAV
                     VSEDANVVSLLETARFYEDFGNATEARSWYYRAYRADFLAGGPDYAKFLLAHGEEREC
                     EKVMLYILSNVKKSTDVARVASVILDKKGKMYHLKRLMEQLVKKLEERRATLNSDGLE
                     LLAIAFFQSATNAEEEMDYAGCKYFCLCGMDVMPAHARSIRLDDYLHLIRTCKERSVA
                     DRPIMNTPQTKKRPVTVPPVTIIADQLGLSEQEQKIVEFLRSHRKASEMDLRKLLGTR
                     RVVGVVNQLIQKASSMGLCLIDKKGVGEDGEVYEYTGT"
     gene            complement(3939..4646)
                     /locus_tag="VFC43_00320"
     CDS             complement(3939..4646)
                     /locus_tag="VFC43_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HZK29669.1"
                     /translation="MTTRPEARYSLVIPAYNEEQRIIPLFDAIHGFEGELIVVCDGVD
                     ATADIVDRIASRRQDLTIRCLRFDHRLGKGGGVIAGLMAAKSPLVGYFDADGSTSIEE
                     MKRLFISLATVDGAIGSRWIPGSTLKVRQSILRQLESRAYNLLIRVLFGLTYHDTQCG
                     AKVFKKTAIDAVLPMMISRGFEFDVELLWRLRGAGFVVAEVPIEWQNKVDSRVRKRDR
                     FRMLSGLFMVRFGFRRP"
     gene            complement(4794..5024)
                     /locus_tag="VFC43_00325"
     CDS             complement(4794..5024)
                     /locus_tag="VFC43_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29670.1"
                     /translation="MQDKEIKEMLADLIWLNALIATELIQITENSSSILRKSPPPESC
                     LAEHHALRETALSMAEKYRPGTMLSQHLGKHQ"
     gene            5122..5541
                     /locus_tag="VFC43_00330"
     CDS             5122..5541
                     /locus_tag="VFC43_00330"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZK29671.1"
                     /translation="MSYLENIKKDGKNANPFFCLMGIDVVSAEPGKAVLKMQVLPVMH
                     NGVGWLQGGILAAIADEAMALALYSLLSKNEGIATISESTSFIRGVQEGDIIAKGRVI
                     RKGRRVAFAEGEVYAGTDERTMLSRTSAAFAVTTNTN"
     gene            complement(5548..6258)
                     /locus_tag="VFC43_00335"
     CDS             complement(5548..6258)
                     /locus_tag="VFC43_00335"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZK29672.1"
                     /translation="MKSGQFIPVLAILLLVSVFVQPIAAAENQTQDAATEFYNNGVNL
                     LEQKEYVRAIVAFDQALASNITMIKQSDALLYTYQNKAYAQIQLNNYTEAVQTIDQGL
                     LLYGNDEKLWYNKGYALFKLGRYQDAITAYDKVLLVNNQSIPALNNKGDTYFQMGKYQ
                     DAVDSYSRANTIDPNDSYSLAGLAKAKSAAATANQTTLVILVIVVVVAAGGLIYYLKF
                     RKPTEQKEPEKKSKGKKK"
     gene            6445..8025
                     /locus_tag="VFC43_00340"
     CDS             6445..8025
                     /locus_tag="VFC43_00340"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HZK29673.1"
                     /translation="MKYIIVTGGVMSGLGKGITAASVGRILKNRGYRVTAVKIDPYLN
                     IDAGTMNPAQHGEVFVLRDGSEVDLDLGNYERFLDIELTAAHNITTGKVYRTVIEKER
                     KGDFLGETVQIIPHITDQIKTCIRQAAEEEFPDGTKADICLVEVGGTVGDIESMPFLE
                     AIRQMRGDLPAQDYILLHVTLIPEDTMGDLKTKPTQHSVKALRELGLHAEIIVGRSEH
                     PISAHTKRKISAFCDVPQHAVISAATIPDIYGVPMEMEKEGIAEVLSAHLGLEKRDVD
                     TSWYRLVTKEYTSRVNVAIVSKYGIEDVYISIKEALKHAGRSLSTEVKIGWLDAERYE
                     RCQLKDYDGILIPGGFGKRGIEGKIEAIRFARENNIPFLGLCLGFQLGVVEFARNKLD
                     VADATSEEFGEGSHVIALLPEQEGLAELGGTMRLGDYTADIKEGTRAMNMYQRKSIIE
                     RHRHRYEVNPRYIEKLEKAGMIFSATNKNRMECLELADHPFYFATQFHPEFKSRPTNP
                     SPPYVGFVEACRVNKRTK"
     gene            8038..8955
                     /gene="guaA"
                     /locus_tag="VFC43_00345"
     CDS             8038..8955
                     /gene="guaA"
                     /locus_tag="VFC43_00345"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HZK29674.1"
                     /translation="MVNTEKFISQAIAEIKTAAGNEKVVMALSGGVDSSVCAALAARA
                     IGDNLIPIYIDTGLMRKGETERIRMIFNTIHLQIVDAGDEFLASLKGITEPEAKRKAI
                     GERFIRVFEREAKKQGATCLLQGTIYPDRIESEGGIKSHHNVGGMPLHMEFTKVIEPI
                     RDLYKDEVREVAGALGLPKEIQHRMPFPGPGLAVRVLGEITKEKVAIIREANWIVEDE
                     LVETYRPWQCFAALLGLGTGVKGDNRIHGWIVAIRAVNSRDGMTADPVEIPFEHLVRI
                     GSRITADIPRVARVVYDITAKPPATIEYE"
     gene            8997..10139
                     /locus_tag="VFC43_00350"
     CDS             8997..10139
                     /locus_tag="VFC43_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZK29675.1"
                     /translation="MEKTSQMKALDERYFMPAFSRDMAIVKGEGSKVWDAEGKEYIDC
                     VAGIAVCSTGHCHPAVVKAICDQAHELIHCSNLYYVPHQGDLAKKLVEITGMHKAFFS
                     NSGAEATDGALKLARVRTGKKKFVAFTHGFHGRTVGSLAVTHKPAIREPFEPLGMVKT
                     FVEYGDLEALKKVVDNDTAGVIFEPIQGEAGVIIPPDSFIEGIREICDESGAYMIVDE
                     VQTGMGRTGKWLAMQHTKVQPDIVTLAKGIASGFPMGALVAREDIEFKKSEHGSTFAG
                     GPLACAAALATIGVIEKILPDVGRNGERFKKGLEKYNPRVRGLMIGATIGDKCPEIQK
                     KCAEEGVLVNCAADGNLRLVPPLVISDDEIDRVVRVLNGALGQVML"
     gene            10111..11169
                     /gene="hisC"
                     /locus_tag="VFC43_00355"
     CDS             10111..11169
                     /gene="hisC"
                     /locus_tag="VFC43_00355"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZK29676.1"
                     /translation="MERLVRSCYKKKSGYVFARKAEEIAREYGIDRIARLASNENPEP
                     VSPAVREAAEKALLTVNRYPDERVNILMNALRSHYGDYHFVTGVGMDGVIETIIRTLV
                     EPREKVVISAPTFSFYALAAMGQGAEIVTVPREADFSVNVKSLIKAAKEAKITVMCSP
                     NNPTGNATSPETVTEILEGIDGVLFLDNAYVEFSDYDYLPLMKKHENLILGRTFSKVH
                     SLAGLRIGYAFTPRWLPPWYNRAGTPFAVNSVSAAAAAASLSDTAHAERCIAQVKRWR
                     KRFESEVKFPVLPSDANFVMVDVAPHTSDEVVESLARKGVVVRSCRSFLGLDDHYIRV
                     SVGDDWENERFLQEINSL"
     gene            11166..11678
                     /locus_tag="VFC43_00360"
     CDS             11166..11678
                     /locus_tag="VFC43_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase family protein"
                     /protein_id="HZK29677.1"
                     /translation="MMCGITGTPGTGKTLVGTELARKGHTVVHLTTTVGPYVTGDDEE
                     RDAQIIDIDRWAAEFVPVDGFVEGHIAHFLPCDRIIVLRCRPDELKKRLSLRKYRKEK
                     IQENADAEALDVCLIETVEAFDPHQIFELDTTGQDVAYCADRIELFVQDQIPSGFGTI
                     DWSGFLEVHS"
     gene            11675..12271
                     /locus_tag="VFC43_00365"
     CDS             11675..12271
                     /locus_tag="VFC43_00365"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZK29678.1"
                     /translation="MTLDQYRTHAKVYFDPLVSIAIKCRMTPNFFTLAAFLASAAAGF
                     LFYFRLELWGIIAVALNAFCDAMDGAVAREMKIQSRRGDFLDHAVDRYADIFIITGIF
                     AGGLVPWPIGVLALTGVLMSSYLGTQAQAVGVGRYYGGLLGRADRLVLILVVGIIDLF
                     IPISFFSLGWLGWLLVMFGVFGHITAFQRFMYIWAKVE"
     gene            complement(12372..12689)
                     /locus_tag="VFC43_00370"
     CDS             complement(12372..12689)
                     /locus_tag="VFC43_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29679.1"
                     /translation="MTGNGRRNSSVEWARSEFEVRYPDLSASIYVTNTKFFRGGNFLI
                     STFDVILPEGTDYYPTAYTGESIITLHRVYAFAFTADNKNFDKYRNLKDRIITSIKIN
                     DLA"
     gene            complement(12786..13208)
                     /locus_tag="VFC43_00375"
     CDS             complement(12786..13208)
                     /locus_tag="VFC43_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29680.1"
                     /translation="MNDRSGTVLFFLFIAAVLAAGCTNQTLPEASVMAPTPSVSIPLT
                     ERTTLVIPTTNPRETTIPTIVPGTTLPPTADTTDVSAITFIQYSDSDFSVEYPAAWNI
                     THSAYFRYFCQNVFDDSPTNYHVCYENETESIGPFNFY"
     gene            13377..>13693
                     /locus_tag="VFC43_00380"
     CDS             13377..>13693
                     /locus_tag="VFC43_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29681.1"
                     /translation="MDKTTIIFSTLCIVALLLAGCTTSSPSPLNGTTITTLPTIAVTT
                     VPVDNQTCTIDADCVPAQCCHPTSCVNQASIHVCNFLCTASCEGPLDCGAGSCGCNNG
                     KCSV"
BASE COUNT         3286 a         3436 c         3639 g         3332 t
ORIGIN      
        1 tgaagggatt aatactattc cggatttgga tctatcatat tcttttttta aacaacggtt
       61 aagcgctcca tctccacctg ttagtcatga tgaacgccgc cgcactgacg gggcgccccc
      121 gcggcggatc agaaaacaaa aaaaactccc tgtccgccgt gcctcggaga ggccctgagg
      181 cgggggtcta tcgtaaaaat cttatcgtga attgctagat acggtcaaac tttcactatg
      241 ctaaaacccg tgtcctatta tctttacccc tgcatccgga acataaccag tcccagtatt
      301 ccggcaatgc ccaccgcaat aaccggtatc acaggttcga gcggtgtttc ggcagccggt
      361 ttatcagatg cgaggaactc tacgtcaacc gttgcaatat ctcccgctac atatctcaga
      421 cgatcaacag gcgaactcac gacatatatc gtatattttc ccggcgacat cacgcccaga
      481 atgacggatg tgtcccaggt ataagtccag gtgccgtcat tcatctgtac cggggccgtg
      541 gtgaataaac cgcgtcctgc ggggacattg aggttgtcca gtgtaactcc ccgtgtgtca
      601 agatcagggc ccgtgacaaa gagatagaca gcgatggtcg taatccctga aacggtcccg
      661 ttcagcgtga caacgtctcc tatatgtgcc tggtgaggtg tggcggagat ggtgagtacg
      721 ggagcagctg ctaccggcat ggcactgatt cccatcagcg caagtaaaat aaccagaagg
      781 tacgcccatc tcataccggt accatacaat ctggttccat aaatcctttt caggcccgca
      841 cttttttaga acctgaccac catgactgcc tcttcttctt ttttcaactc gcctttgagg
      901 tgctcggtat cgaaatcata ggcatcagcg ggattatagt cctcatagag ttcgcactta
      961 tcaagcacat cgacaaactc ttttaaaaat atccgcggga tcacgtccac acgcccgcca
     1021 aaccgggtgg tgatcttctt aatcatcgca cgcacgaacc ggtgcgatac ccgttcacgg
     1081 tcaggttcgg aataagcctg tgcgtacacg tccactacct tgagcccaac cagctcgagc
     1141 ttggaggtgt caaacttctc aaggatgatc tggggctggc gcgggttgcg gaatgcatcg
     1201 gtctgtacaa cattaatccg gtcataaagg ggtggaatag accggatgcc ccgtgatccg
     1261 tcataaagag caggtgttcc ggtaaagagg aagtagcagc gcggcatctc gccccggtcc
     1321 agggcatcaa tgatctggac gagggtctgg taaccttttt cccgctggtt gcgctggagc
     1381 ccctgcgtag tttccatttc atcgacagca atcgcgatcc cgcggtagcc cgccccatgg
     1441 ataatggaca cgagcccgcg aagaaaaata aacgcgatgg tatcatcgat atctccccgg
     1501 atcccagcct tctgcttgaa gtccctgccg atattgggtt cagcagagat ccagcccagt
     1561 gctgactggg caagctggaa ttccccggca ttgtttgcct tatagtacgt gcgaagcgcg
     1621 gcagcaaggg ccgtgtttac ctcactgatg ccggcaagag aggcctcgat ctcccgctcg
     1681 gtggcccctt ccagggccaa atcctcccct gcagtcccgc tcacggaaat caggcgctct
     1741 tcaatggagt agagccagtt atcgattatg gttttgaggg cgtgttcctc atcgctcgtc
     1801 ctgaggttcg cacagatctg ctggtatatg cctttgagtt tgtggagggg ggttgacgag
     1861 gagataatca cgtgggaggt gacaaaaccc ttggtgcgtg cgatctcgag agcccgggcg
     1921 acaagaaacg tcttgccgct gccgtattcg ccccggatga atttggtatc gcctcctccc
     1981 tgggcaacgt aatccagctg tttgcttatt acaccttctt cgatatcaag cccgacagcg
     2041 atccgctcaa gcccgcctgc aggcaccgtt ccccgtctca aagcattgat gatattcatg
     2101 ctttcgagcc ggcgccggtc catctgctca ggtaccggtg tattcataga cttctccatc
     2161 ttctccaacc cctttcttgt cgataagaca caatcccatc gacgatgctt tctggatcag
     2221 ctggtttact actcctacga ccctgcgggt acccagcagt ttccgcagat ccatctcgct
     2281 tgctttccgg tgcgaccgga ggaattcaac tatcttctgt tcctgttctg acagcccgag
     2341 ttgatcggca atgatcgtta ccgggggaac cgtaaccgga cgtttctttg tctgcggtgt
     2401 attcatgatg ggccggtcgg caaccgatcg ctccttgcat gtccggatca ggtggagata
     2461 atcatcaagg cgtatggacc gggcatgcgc cggcatcaca tccatgccgc agagacagaa
     2521 atacttgcag cctgcatagt ccatctcttc ttcagcattg gttgcgcttt gaaaaaaagc
     2581 aatagcaagc agttccagcc cgtcagagtt taacgtcgct cttcgctcct cgagtttttt
     2641 tacgagctgt tccattaagc gtttcaggtg gtacattttc cctttcttat caagtatgac
     2701 agaagctacc cgcgcgacat cggtgctttt tttgacattg gacaggatat agagcatcac
     2761 tttttcacat tcccgctcct ccccgtgtgc aagtaaaaat tttgcatagt caggcccacc
     2821 ggctaaaaag tctgcccggt acgcccggta ataccatgac cgtgcttcgg tagcattgcc
     2881 aaaatcctca taaaagcggg ccgtttccag gaggcttaca acattagcat cttcagaaac
     2941 aacggccagg aaccgcttgc gggcttcctc cgtggggaca tgtgctttaa gataccggcg
     3001 gtacttatcg ataatacggg cgaggagatc caggtcgttt ttcgagccaa gctcgtaacg
     3061 gatcaggttt tcataggatt ccagtgcttt gctcgcgtcc cccgatgctg caaggagatc
     3121 gcatccgacc agctggtaga gcggatcacc cgcacggtcc aggaggggtt taagagcagc
     3181 aagtgccggg gcataatccc cctttgcctg taaaagcagg atataatcaa acaggatttc
     3241 ctcatccggc tctttttgga tgtgcgattc gtatgcacca agggatgcct gcatatcata
     3301 tcgggacagg gtatcaaggt attttctatg gatttgagga tcctgcgtgt cgcggtatat
     3361 ccggtatgcc agttcggcag ccgccgggaa attttgtgtc ttgatgagag catcaatgta
     3421 ttcgggagtc ttttgcgcat cacccccgag tattgcagat atggcaagtg cttcctgtgg
     3481 ctcaccaatc tctatgagca cacgcataag gtgctggacg tctgctggtt ttttcccgag
     3541 ttggaccagg cgccgtagca cgggcaggga accacggtag tccttatgcg agataagata
     3601 tgctgcatag ctcctgagag cgtcctggtt ggtgggatcg agatggacag caagtgcata
     3661 ctcggtaatc gctccttcat ctttcctggc ttcaagtact ttacccaggt agctatggat
     3721 atatgaggac tcctgcatct tctggctcag gtcacggaag aatacttcag catcttcaaa
     3781 tctgcccgtg taatagaggt tcgtggcagc aaggacttca actgcagagt ccttttcccc
     3841 ctcaaggagg gcgttgcaga cgtcgaggga ttcctggtac ctcccgtctt caaagagcct
     3901 gaatgcttcc ttcttccgat catcaatgga tgcgggaatc atggtcgcct aaaaccaaac
     3961 ctgaccataa aaagaccgga aagcatcctg aacctgtctc gtttccgtac cctggagtct
     4021 actttattct gccattcgat ggggacttcg gcaaccacaa atcctgcacc ccggagtctc
     4081 cagagcagct cgacatcgaa ttcaaatccc cgtgatatca tcatcggtaa tactgcatca
     4141 attgctgttt ttttaaatac ctttgccccg cactgcgtat cgtgataggt taatccgaag
     4201 aggactctga tgagaaggtt gtaggcacgg ctctccagct gccgcaggat gctctgtcgc
     4261 actttgagcg ttgaaccggg gatccagcgc gatccaatcg caccatcaac ggtagcaagt
     4321 gatataaaaa gccgtttcat ctcctcgata ctggtagagc catcggcatc gaagtacccg
     4381 acaaggggag atttggccgc catgagaccg gcaattactc ctcccccttt tccgagccgg
     4441 tgatcgaaac gcaggcaccg gatagtcaaa tcctgccgtc gtgatgcgat acggtccacg
     4501 atatcggccg ttgcatccac tccgtcgcag acaacgatga gttccccctc aaacccgtgt
     4561 atcgcatcaa agagtgggat aatcctttgt tcctcgttgt atgcggggat gacaaggctg
     4621 tagcgtgctt cgggtcgggt agtcatgatg tacaaggtat gttgttttgt cgataatcaa
     4681 caatgaaaac tcttttttcc ggaagtggag gatggggttc cttgctgcat atgctgatat
     4741 acaaaattac atcagttccg ttacagtact tttcgcccga tctccccata actctactgg
     4801 tgttttccga ggtgttgtga gagcatggtg ccgggacggt atttctctgc catggaaagt
     4861 gccgtctcgc ggagcgcatg gtgctcggcc aggcagcttt ccgggggcgg ggattttctc
     4921 aggattgatg aactgttttc ggttatctgg ataagttctg tcgcaatgag ggcgtttaac
     4981 cagattaaat cggcgagcat ctctttgatt tccttgtcct gcatgcagaa tacgtcattg
     5041 tgcagatgat ataaacagat tgcatgggtt agatgagagc aatcttaatc cctttttatg
     5101 gtaccatcag gtaggggaat aatgtcgtac ctggaaaata tcaaaaaaga cggaaaaaac
     5161 gcaaaccctt ttttctgcct tatggggatc gatgtggtca gcgcagaacc aggaaaagcg
     5221 gtgctgaaaa tgcaggtcct tccggtaatg cacaatggtg tggggtggtt gcaggggggt
     5281 atactggcag caattgctga tgaagcgatg gcccttgccc tctactccct gctctcgaaa
     5341 aacgaaggaa tcgcgaccat ctctgaatca accagtttta tcaggggggt ccaggaaggg
     5401 gatattattg ccaaaggccg ggtaatcagg aaggggcgac gggttgcatt cgctgaaggt
     5461 gaagtgtacg cgggaacgga tgaaaggact atgctttccc ggacgtcggc agcatttgcc
     5521 gttacgacga atacgaatta aaaaaagtta ttttttcttt cctttggatt tcttctccgg
     5581 ttctttctgt tcggtgggtt tccggaattt caggtaatat atcaatccac cagctgcgac
     5641 aaccacgact ataacgagaa tgacaagcgt cgtctggttc gctgttgcgg cagcactttt
     5701 tgcttttgca agtcctgcga gggagtagga gtcattcggg tctatcgtat ttgccctgga
     5761 ataactatcg acagcatcct ggtacttccc catctggaaa taggtgtccc ccttgttgtt
     5821 gagggcggga atggactggt tgtttaccag aagtacctta tcatacgcag tgattgcatc
     5881 ctggtatcgt cccagtttga agagagcata gcctttgtta taccagagtt tctcatcatt
     5941 tccatagagt aaaagcccct ggtcgatcgt ctggactgct tctgtataat tattcagctg
     6001 gatctgtgca tacgctttgt tctggtaggt atataacaga gcatcggatt gctttatcat
     6061 tgttatattc gaggcgaggg cctgatcgaa tgccacaatc gcccgcacat attctttttg
     6121 ttcgagaagg tttacaccat tattgtaaaa ctccgttgcc gcatcctgtg tttggttctc
     6181 tgctgccgcg attggctgga cgaaaacgga aaccagcaag agaattgcca gaacagggat
     6241 aaattgcccg cttttcatat ctactaggtt tgagttccgg gatacatgaa tcttgcttca
     6301 tcctgctcaa gacccggaat gcggactggc aagatcttcg ggtcaacaaa aaaccatcag
     6361 gcccacgaaa tcctcaggta tcgaatgata agataacact aataggtatt atgcgcaaaa
     6421 tgtaaagcaa tcggggagtt tagagtgaaa tacatcattg ttaccggcgg tgtgatgagc
     6481 ggattgggaa aagggatcac cgcagcttct gtcgggcgta tcctgaagaa ccgtggctac
     6541 cgggttactg ctgtcaagat cgatccctac ctgaatatcg atgcggggac gatgaacccg
     6601 gcacagcatg gcgaagtgtt cgtgctcagg gatggcagtg aagtcgatct cgacttaggt
     6661 aactacgagc ggtttttaga tatcgaactc acagctgccc ataatattac caccggtaaa
     6721 gtgtaccgca cggtcattga aaaggaacgg aaaggagatt tcctcggcga gaccgtgcag
     6781 atcattcccc atatcacgga ccagataaag acctgcatac gacaggcggc agaagaagaa
     6841 tttcccgatg gaacgaaagc cgatatctgc cttgttgaag tgggcggtac tgtcggtgac
     6901 atcgaaagca tgccgtttct tgaggcaatc cgccagatga gaggagacct gcccgcccag
     6961 gactatatcc tcttacatgt tactctcatc ccggaagata cgatgggaga cctcaaaaca
     7021 aaacccacgc agcactcggt aaaagccctg cgggaactgg gcctccatgc tgagataatc
     7081 gtcgggagga gcgagcaccc gataagcgct catacgaaac gcaagatctc tgcgttctgc
     7141 gacgttcccc agcatgcagt catatctgca gcaacaatcc cggacatcta cggggtcccg
     7201 atggaaatgg aaaaagaagg tattgcggag gtactttctg cacacctcgg gcttgaaaaa
     7261 cgtgacgttg ataccagctg gtaccgcctc gtgaccaagg agtataccag ccgggtcaac
     7321 gtagcgatcg tcagtaaata cggtatcgag gatgtataca tcagcatcaa ggaagcgctc
     7381 aagcatgcgg gcaggtcgct ttcaacggaa gtaaaaatcg ggtggctgga cgctgaacgc
     7441 tacgagcgct gccagctgaa ggattatgac gggatattga tacccggagg gtttggcaaa
     7501 cggggtatcg aaggaaagat agaagcgatc cggttcgccc gtgaaaacaa catccccttc
     7561 ctcgggctct gcctcgggtt tcagcttggg gttgttgaat ttgcccgcaa caaactcgac
     7621 gttgccgatg ccacaagcga ggaattcggg gaaggttcgc atgtcatcgc ccttctcccg
     7681 gaacaggagg ggcttgcaga acttggcggt acaatgcgcc tgggtgacta tactgcagat
     7741 attaaggaag gcacaagggc gatgaacatg taccagcgga agtctattat cgagcggcac
     7801 cggcaccggt atgaagtgaa cccgcggtat attgagaaac tggaaaaagc ggggatgatc
     7861 ttctcagcaa ccaataagaa ccggatggag tgcctggaac tggctgatca tcctttctac
     7921 tttgccaccc agttccaccc ggagttcaaa tcccgcccga caaatccgtc acccccctac
     7981 gtgggttttg tcgaggcatg cagggtaaat aaacggacaa aatagggaca cgataccatg
     8041 gtaaatactg aaaaatttat cagccaggct attgcggaaa taaaaactgc cgcaggtaac
     8101 gaaaaggtgg ttatggcctt gtcgggaggt gtcgacagct cggtctgcgc tgctcttgca
     8161 gcccgtgcca tcggtgacaa tctcattcct atttacatcg ataccggcct gatgcgcaaa
     8221 ggcgagacag aacggatcag gatgattttt aacaccattc acctgcagat agtggatgcg
     8281 ggtgatgaat tccttgcctc cctcaaggga attaccgaac ctgaggcaaa acgcaaagca
     8341 atcggtgaac ggttcatccg agtctttgag cgggaggcaa aaaaacaggg tgcaacctgc
     8401 ctgcttcagg ggactatcta cccggaccga atcgagagtg aagggggtat caagagtcac
     8461 cataacgtcg ggggtatgcc gctgcatatg gaatttacca aggttattga gccgatccgc
     8521 gatctctaca aagacgaagt aagggaagtg gcaggagctc ttggcctgcc aaaggaaatc
     8581 cagcaccgga tgccattccc cgggcccggg cttgccgtcc gggtgcttgg ggagattacc
     8641 aaagagaagg tcgcaatcat acgggaagca aactggattg tggaagatga actggtggaa
     8701 acctaccgcc cgtggcagtg ttttgcagcc cttctcggtc ttgggaccgg ggtgaaggga
     8761 gataaccgta tccacggatg gattgttgca atccgggcag tgaactcacg ggacgggatg
     8821 actgcagatc ctgtcgagat cccctttgag catctcgtga ggatcggttc aagaattact
     8881 gctgatattc cccgtgttgc cagggtagtg tacgacatta ccgccaaacc cccggcaacc
     8941 atagaatatg aataaactga acaaaagaaa aaagatacta caatcaagtg atttgaatgg
     9001 aaaaaaccag ccagatgaaa gcgctcgacg agcgctactt tatgccagcg ttctcgcgtg
     9061 atatggctat cgtaaagggt gaaggttcaa aagtctggga tgcggaaggt aaggaatata
     9121 tcgactgcgt tgcaggaatt gccgtttgca gcaccgggca ctgccacccg gctgtcgtga
     9181 aggcaatctg cgaccaggcc catgaactca tccattgctc caacctgtac tatgttcccc
     9241 accagggaga ccttgcgaag aaactggttg aaataaccgg tatgcacaag gccttcttct
     9301 ccaattccgg tgcggaggcg accgatgggg ccctgaagct tgcccgtgtc aggaccggta
     9361 aaaagaaatt tgttgcgttt acccatgggt tccatggccg tactgtcggt tcactcgcag
     9421 tcacccacaa acctgcaatc cgtgagccct tcgaacctct tgggatggtc aagacctttg
     9481 tcgaatatgg tgatctggaa gcactgaaaa aagtcgtgga caacgatacc gcgggcgtca
     9541 ttttcgaacc gatccaggga gaagcagggg tcatcatacc cccggacagt ttcattgaag
     9601 gaatccgtga gatctgtgat gaatccggtg cctacatgat cgtcgatgaa gtccagactg
     9661 gcatggggag gacgggcaaa tggcttgcca tgcagcatac gaaggttcag cctgatatcg
     9721 taacccttgc aaaagggatc gcgagcgggt ttccgatggg tgcattggtt gcccgtgaag
     9781 atatcgagtt caaaaagagt gaacacggga gcacgtttgc cgggggacct cttgcctgtg
     9841 cggcggcact tgccacaatt ggtgtcatcg agaagatcct ccccgatgtc ggccggaatg
     9901 gagagcggtt caaaaaaggg ctggaaaaat acaatccccg ggtccgcggg ctgatgatcg
     9961 gtgcaaccat cggggataaa tgcccggaga tccagaaaaa atgtgcagag gagggagttc
    10021 ttgtgaactg tgcggcggac gggaacctgc gtctggtgcc cccgcttgtc atctccgatg
    10081 atgaaattga ccgtgtggtc agggtgttga atggagcgct tggtcaggtc atgctataag
    10141 aaaaagagcg gctatgtttt tgccaggaaa gcagaagaaa tagcccgtga gtacggtatc
    10201 gaccggatcg cacggcttgc cagcaatgaa aatcccgaac ccgtttcacc cgcggtacgg
    10261 gaagctgcag aaaaggcact cctgaccgtg aaccggtacc ctgacgaacg tgtcaacatc
    10321 ctgatgaacg cgctgaggtc acactatggt gactaccact ttgtgacggg tgttggcatg
    10381 gacggggtca tcgagacgat catccgcacg ctggtcgaac ctcgcgagaa agttgtcata
    10441 tcagcgccca ccttctcctt ttatgccctt gcggcaatgg gacagggggc cgagatcgtc
    10501 accgttcccc gggaagcgga cttctcggtc aatgtaaagt ccctgataaa ggcggcgaaa
    10561 gaggcgaaga ttaccgtcat gtgctctccc aataacccga ccggaaatgc caccagccct
    10621 gagacggtca cagaaattct tgagggaatc gacggggtgc tcttcctcga caatgcctac
    10681 gttgagttct cggactacga ttaccttccg ctgatgaaga aacacgagaa cctcatcctt
    10741 gggcggacat tctcgaaggt gcattccctt gccggactcc gtatcggcta tgcattcact
    10801 ccgcgatggc ttcccccgtg gtataaccgc gccggtaccc cgtttgcggt caactcggtc
    10861 tccgctgctg ccgctgccgc ttccctgtcc gatacggccc acgccgaacg gtgtattgcc
    10921 caggtaaaac ggtggaggaa acggttcgaa agcgaggtga agttcccggt cctcccttcg
    10981 gatgcaaact tcgtgatggt agatgtcgcc ccccacacaa gcgatgaagt tgtagagagt
    11041 ctcgcccgca aaggtgtggt tgtccgttcg tgccggagtt ttttaggcct tgatgaccac
    11101 tacatacggg tcagcgtggg cgatgactgg gaaaacgagc gcttcttaca ggagatcaat
    11161 tcgctatgat gtgcggcatc accggaacgc ccgggaccgg taaaacgctg gtgggtaccg
    11221 aacttgcccg aaagggacat actgttgtcc accttaccac gacggtcggt ccgtacgtta
    11281 ccggtgatga cgaggagcgg gacgcacaga ttattgatat cgatcgctgg gcggcggaat
    11341 ttgttcccgt ggacggattt gtcgaggggc acatcgcaca ctttctcccc tgcgaccgta
    11401 tcattgtgct ccggtgccgc cctgacgaac tgaaaaaacg cctttccctc cggaagtacc
    11461 ggaaagagaa gatccaggaa aacgccgatg cggaagctct cgatgtctgc ctgatcgaga
    11521 cagttgaggc atttgaccct caccagatct tcgaactgga tacgactggg caggatgtgg
    11581 cgtattgtgc agaccggatc gagttgtttg tccaggatca gataccctca gggtttggca
    11641 ctatcgactg gtccgggttt cttgaggtgc attcatgacg ctcgaccagt accgcaccca
    11701 tgccaaagtc tactttgacc cgctggtttc catcgcaata aaatgcagga tgactcccaa
    11761 tttttttacc cttgcggcgt tccttgcttc ggcagccgcc ggattcttat tttatttccg
    11821 gcttgaactc tggggaatta tagcggttgc cctgaatgcg ttctgtgatg caatggacgg
    11881 tgccgttgcc cgcgagatga agatccagag ccgccgtggg gatttccttg accatgcggt
    11941 ggaccggtat gcggatatat tcattatcac gggtatcttc gcgggcggcc ttgtcccctg
    12001 gcctattgga gtccttgcac tgacgggcgt cctgatgtcg tcgtatctcg gcacgcaggc
    12061 acaggccgtg ggtgtcgggc gctattatgg cggactgctc ggcagagcag accggctcgt
    12121 gctgatcctt gtcgtcggta tcatcgacct tttcattccc ataagtttct tttcgcttgg
    12181 gtggctgggc tggctgctgg tgatgttcgg ggtgtttggg catattaccg cgttccagcg
    12241 gttcatgtac atctgggcaa aggttgagtg aggattataa cctgccctgg atgaaatatc
    12301 cgaagatgcc tttgcgcttc ctgatctaac aggaaaagga gaaaaactgt tttttttatc
    12361 aaaaagttag ttcaggcaag atcgtttatt ttgattgagg taattatccg gtccttcagg
    12421 tttcggtatt tgtcaaaatt tttattatcc gcagtaaatg caaacgcgta tacgcggtgc
    12481 agggtaatga tggattcccc ggtataagct gtcggataat agtctgtacc ttccgggagt
    12541 atgacatcaa atgtggagat caggaagttc ccgcctctga aaaatttcgt attggtgaca
    12601 tagattgatg cggacaaatc cggataccgc acttcaaact cacttctggc ccattcgacc
    12661 gatgaattac gtctcccgtt accagtcacc gtatccagga aatctgaggt aaacgatata
    12721 aattttaatg taccgtcggc actcgtaaag gtgacaaccc gtgatggctt cctgagtgtg
    12781 tcatcctagt aaaaattgaa aggtccgatg gattctgtct cattttcata gcatacgtgg
    12841 taattcgtag gactgtcatc aaacacattc tggcagaagt agcggaagta cgctgaatgc
    12901 gtaatattcc atgctgcagg gtattcaacg ctgaaatcgc tgtcggaata ctgaatgaag
    12961 gtaatggcag acacgtcggt agtatctgcc gtagggggca gggttgttcc tggtacgatg
    13021 gtcgggattg tggtctccct gggatttgtg gtgggaatta ccagggtggt tctttcagta
    13081 aggggaatcg atactgaagg tgttggcgcc atgacggatg cttccggtaa cgtctggttt
    13141 gtacaccctg ctgcaagcac agcggctata aagagaaaaa aaagaacggt tcccgaacga
    13201 tcgttcatac tatttcagat cgtagtatca gacgtttaat agtattgttc tgctaataat
    13261 gcctgatccg gacttttttt aagcaccatt ctatactgtc tttttgcccg gtaataccct
    13321 ggaatccaga tacaatcccg gtggtttaat aatagttgga ctggtaatcc cattacatgg
    13381 acaaaaccac gattattttt tctaccctct gcattgtggc actcctcctg gccgggtgca
    13441 cgacatcctc tccttcacca ttgaacggga caacaatcac aaccctcccg acaattgcgg
    13501 taacaacagt gcccgttgat aaccagacct gtaccatcga tgcagactgt gtgcctgccc
    13561 agtgctgtca cccgaccagc tgtgtgaacc aggcatcaat ccatgtatgc aatttcttat
    13621 gtactgcatc ctgcgaggga ccgctggact gtggtgccgg cagctgcggg tgtaacaacg
    13681 ggaaatgttc ggt
//
LOCUS       DASRLV010000010         2739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_571015,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000010 DASRLV010000000
VERSION     DASRLV010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571015"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            390..842
                     /locus_tag="VFC43_00385"
     CDS             390..842
                     /locus_tag="VFC43_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HZK29682.1"
                     /translation="METLIVSLDFLEFPPTHTCEGDNLSPRIRLKGLNAASIAVMVFN
                     PFEKSCCSFTPWIIWNIPPVSIIPAGIPREGIVTAPVIAVQGITDHGSLGYTGPCPPH
                     GQMIRYQFKVYGLDSMLDLKPGSDKHELVSAMKGHVVQFGETVGICSR"
     gene            928..1389
                     /locus_tag="VFC43_00390"
     CDS             928..1389
                     /locus_tag="VFC43_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HZK29683.1"
                     /translation="MEELKVKISVKVLPNDYTCDGQDISPEIDVGGVNVKIAKSLAIM
                     VNDPDAPGGGGFVHWVAWNMELVRLIPEKIPKTPVVTFPLKLVQGKNSFGKIGYNGPC
                     PPQGQTHRYYFKVYGLDTELSLAAGATKDQLVRAMEGHVVQYGETFVTYGR"
     gene            1770..2012
                     /locus_tag="VFC43_00395"
     CDS             1770..2012
                     /locus_tag="VFC43_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29684.1"
                     /translation="MDATVSSEEITSMMQATTNAKNLLKKMQVKVVTNNEMEMSAFAD
                     LESYGYHLRGPVYLQRYLFKKGSLSGSVEFLSDSSG"
     gene            complement(2274..>2739)
                     /locus_tag="VFC43_00400"
     CDS             complement(2274..>2739)
                     /locus_tag="VFC43_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29685.1"
                     /translation="DYLAEDYRNTVELTEIMDSLRERINLDEVPHFTTIHKFSQRINS
                     FVFTRLLNRLIKQFYDWGEKISCTAIDSSGFTSSYASSYYSWRTGKTRKRFLKTSISV
                     DTDRQIITGFKISQQPVHDIPHAEKLLVHVTERESLILMFSIKDTILKIFTD"
BASE COUNT          750 a          614 c          630 g          745 t
ORIGIN      
        1 cctcagcctt gggtggcagg ccggcagggc tgttttttgc cgaagagcag gttttggcag
       61 tagcgcatgt ggaacactcg tttttgcatt cttcatcctc tgccttcgtt ctggtatctt
      121 tttttgtcat gggatgcctc ttttaaaaaa attatctgtt attactctca tcaattatcc
      181 tttgcatgca ataaagatgt ggagatggct gcaatcacaa acagttgttt cacgttgcct
      241 tatcctgtca acagcagccg gacatgattt gatgcagatt accggggttc catcaggtgc
      301 atcaagccac cgcatgaaca aaaaaagacg ggtgcctgaa aagaccaagc agacaaagca
      361 ttatttttcc tgtactccat tttgtagtaa tggaaaccct tatcgtatcc cttgatttcc
      421 tcgagttccc acccacgcat acctgtgagg gggataacct ctctccccgg atcaggttaa
      481 aaggcctgaa cgccgcatca atcgccgtta tggtgttcaa tccttttgaa aagtcctgct
      541 gttcattcac tccctggatc atatggaaca tccccccggt atctataatt cccgcgggta
      601 ttccccgtga aggtatcgtt actgcacctg ttatagcagt acagggtatc actgaccatg
      661 gttctttagg gtataccgga ccctgcccgc cacatgggca gatgatacgg taccagttca
      721 aagtatacgg gctcgacagc atgctggacc tgaaacccgg atcagacaaa cacgaacttg
      781 tctctgctat gaaaggacac gttgtacagt ttggcgaaac cgttggaatc tgttcccgtt
      841 aaagaaaaaa attctcctgc attaaactgg tgcgcaaaaa ttataatgcg cccggcatat
      901 cattcacaca acaaggaggc attatctatg gaggaactga aagtaaaaat ttccgtgaaa
      961 gtccttccca atgactacac ctgcgacggg caggatattt cgcctgaaat tgatgtaggg
     1021 ggagtgaacg taaaaattgc aaaatccctg gcgattatgg taaatgatcc cgatgcgccg
     1081 ggtggcgggg gttttgtcca ttgggttgca tggaacatgg agctcgtcag gctgatcccg
     1141 gaaaaaattc caaagacacc cgtcgtcaca ttccccctta aactggtcca gggaaaaaac
     1201 agctttggaa agatcgggta caacggcccc tgcccacctc aaggacaaac tcaccggtac
     1261 tactttaaag tctatgggct tgataccgaa ctttcccttg cagcaggagc gacaaaggac
     1321 cagcttgtcc gggcaatgga aggccatgtc gttcagtatg gtgaaacttt tgtgacctac
     1381 ggaaggtaaa aaacatccct gcactttttt cctcgttaat ataatcaatt cagatataaa
     1441 acgacgattt tgatttatta ataaaaaaga atcgcctaat accaacaccc aggagaggtt
     1501 catgtcggaa acaaaaaacc cgttcttctc tttcagggtt attacctaat cctcgtagcg
     1561 ctcatcgttg tgatcataat agcagcttcg ggaattgtat atattcccgg attttccgaa
     1621 gtccttggta caaacaaacc ccgtgatctt ggcataaaag cagatccggc acgattcagt
     1681 gcgctctttc acgggagaat gtaaaactca ccggccagac ttcagacttt tacctgtcgg
     1741 cacctgtcag atatggatca gcagtcccca tggatgcaac ggtctcaagc gaagaaataa
     1801 cctcgatgat gcaggcaacc accaatgcga aaaacctgct taaaaaaatg caggttaaag
     1861 tcgtcaccaa taacgagatg gagatgtctg cttttgctga tctggaatca tacgggtatc
     1921 atctcagggg cccggtttac cttcaaaggt acctttttaa aaaaggcagc ttgtcgggct
     1981 ctgtagaatt cctcagcgat agttccggat aaagattgcg agaataattt tagagatgtt
     2041 atagagaatc aatttgattt tgagttcttt gatctgtgat tggaattttc gtgctttcat
     2101 ggattccccg aactttcttt taaggacgga aaatacggtt tccaccaggt ttcttcgatg
     2161 gtataatgtc aggtcaaata ctcgtgagag ttcacggcgg tagtaaccag agatccgttt
     2221 tcgttttcgg tcacggaccg ggatgagaga atacgagttc aaatcatccc ggatcagtcg
     2281 gtgaatatct tcagaatcgt atcctttatc gagaacataa gtatcagact ttctcgttcg
     2341 gtgacatgaa cgaggagttt ttccgcatga gggatatcat gaaccggttg ttgggaaatt
     2401 ttgaacccgg taatgatctg ccggtcggta tcaacggata tagaggtttt caggaatcgt
     2461 tttcgggttt taccggttct ccaggaataa tatgaactgg cataagaact ggtaaaaccc
     2521 gacgaatcga tcgcggtgca agagattttc tcaccccaat catagaactg tttaatgagc
     2581 cggttcaaca atcgggtgaa aacaaacgag ttaattcttt gagagaattt atggattgtg
     2641 gtgaagtggg gtacttcgtc gagattgatc ctctctcgga gagagtccat gatctcggtg
     2701 agttcaacgg tattccggta atcttcggca aggtaatcc
//
LOCUS       DASRLV010000011         6056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_815503,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000011 DASRLV010000000
VERSION     DASRLV010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815503"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VFC43_00405"
     CDS             <1..691
                     /locus_tag="VFC43_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyl transferase"
                     /protein_id="HZK29686.1"
                     /translation="MVSTGIFSLTKDFFSFTSENNITDAVSSMLASGEHLKAVVADDW
                     QDAIYPWDLLKMNMRLLKDLPEARGGTVSRQTIIQGPVGIGKGTIIGPNTIISGPVII
                     GNDCEIGPNCCIMPNTSLGSRVKVEPFTLIGKSLIMDDTSIGSHSRIIDTVTGQGCRL
                     ADHTTISASASLMNIEEEVIKPEFGAVFGDQVTAGPFTIFKNCIVGNNVAIEGGSRVI
                     SRILPDESKVI"
     gene            693..2438
                     /gene="glmS"
                     /locus_tag="VFC43_00410"
     CDS             693..2438
                     /gene="glmS"
                     /locus_tag="VFC43_00410"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HZK29687.1"
                     /translation="MCGIVGYIGRREAAPIIVEGLKKLEYRGYDSFGVATISGGIELD
                     KHQGRISDLSDSAVCLKGKIGIGHTRWATHGVPNDVNSHPHLDCSGRIAVVHNGIIEN
                     YSELKRQLISRGHTFLSETDTEVVAHLIEEAYEPEKDLLSAVQKVVPKLTGSYALLVI
                     ATGEDRIIAARYASPLVLGIGDGEFFAASDMTPVIDHTERAIFLEDGDVASISYEKAE
                     IFHDGRPVKRPLELIDWSGEDTKKGGFPHYMLKEIYEQPQAFYNSVRSVTNELIPAPV
                     KNAESVTIIACGSSYNAGLIFKYLLEDTCAIPVRIDFASEFKYFPPPIRGLVIGISQS
                     GETADTIAALKQAKVHNCTTLAITNILGSSISRAVDFTLFMRAGPEISVAATKSFIAQ
                     LAVLMQVTNFLRNGEFEDVLRNGHRAIEEVLLIDLSDAVGLCRNARSIFYVGRGPFYP
                     VSLEGSLKMKEISYIHAEGYAAGELKHGPFALLSPETPVVAICMPGETYGVMISNIKE
                     MKARGAPVIAIGEEGDLELSEVVDIFVPLPVTHPFVQVLTSLIVLQLLAYYTAVGLNR
                     DVDKPRNLAKSVTVE"
     gene            2435..3043
                     /locus_tag="VFC43_00415"
     CDS             2435..3043
                     /locus_tag="VFC43_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HZK29688.1"
                     /translation="MIEYGINTVGTGAQVFEPVTLGFPSRDKMGQTGFTGTIIGKNAV
                     IRSGTIIYCDVTIGDNFQTGHNVMIREKTKIGHRVAIGTSVVIEGNTVIGDEVSLQSM
                     VYIPTDTIIGNNVFIGPNTVLTNDRYPPTRIGGLKGPQIRAGAAIGANATLLPGVCIG
                     EGALVAAGAIVTRDVPAHMLAIGAPAKITDLPQKMVQEVHPK"
     gene            3238..5832
                     /locus_tag="VFC43_00420"
     CDS             3238..5832
                     /locus_tag="VFC43_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006487 - protein N-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharyl transferase, archaeosortase A
                     system-associated"
                     /protein_id="HZK29689.1"
                     /translation="MVLIDLKNHRTQLIIGLVSLFCVFALWLRLLPMLNMGNTDVLMM
                     VASDDPLYNLRQVEQILANFPSYAWYDPMTLFPVGMSVPWGPLFPTIIALFCIITGAT
                     TRPEIISTGLLIPPLLGVMIVAIMYFVGKTCGDWKTGLLASGFTAIVSGQFFYRSFYG
                     YMDHHIAEVLFSTIFCLLYMTAVFSEKDIKIDLKNILSYKKTLLLSGLAGIAYLLGLF
                     VMPTMILFAMIVGIFTIIQFVIDVARGKTSEYLLVINTIIFTIAIIGLLMNGLKDPGI
                     NLIFYSVGHVYAYLALIGGTCFLYGVARFVKEKNWYYYPAVLIGSGIAFSLILLIVSP
                     QLYNLFINSLFSFFGQQAITNTVQEARGWAPDLAWTTFNYGLILMVGGALVMLYKNLK
                     EEHPHQIFALVWSLVMLVSTWQHIRYEYYLAVNVALLSAVCVSFTFDRGWPEIRKLAS
                     RISSDTDVQEFNKGGKEEPQKGKKQKKIHGKSSVATQSNFLVIVFVILTIGLSILFAY
                     TSASLSYGNAARDTIRMNPDWRETLEWMGNNTPDTGVNYTTIYNPHTFQYPPEAYGVM
                     SWWDYGNMITYIAKRIPNANPFQAGVTGPTGSANFFMSVSEDDGDKVLDLAKTRYIIT
                     DIEMDTGKFWAMATWYNASAGANPYQMTLLTPAQNTLNSYESVLLNTRLYYLTMISRL
                     HNFDGSLTTPTSAYYIEYTDPTISQVSLPVITGAEALNASDALLRAAEYNLNAPAGYH
                     AAALSPALTLPVETVPALRHYRLVYESKSNVFNSNTTDVKYVKVFEYVKGAHIQGEGI
                     IQVPVISNTGRTFIYRQMSSNGEFIVPYSTTGSTYGVKTSGKYKVISSGREYDVPESA
                     VMEGALIR"
BASE COUNT         1584 a         1424 c         1457 g         1591 t
ORIGIN      
        1 catggtaagc accggcattt tttcgctcac aaaagacttt ttttcgttca cctctgaaaa
       61 caacattacc gatgccgttt cctcaatgct tgcttcgggg gaacacctga aggcggttgt
      121 tgcagatgac tggcaggacg ctatctatcc ctgggacctg ctgaagatga atatgcgtct
      181 gttaaaagat ttgcccgaag cacgcggagg gacggtaagc aggcagacca tcatccaggg
      241 gccggtggga ataggaaaag gtaccatcat cggtcccaat acaataatat ccggaccggt
      301 tatcatcggc aatgactgcg agattggccc caactgctgc atcatgccga atacgagtct
      361 tggttcccgg gtcaaggtag aaccatttac gttgatagga aaatcattaa tcatggatga
      421 cacatccatc ggttcgcatt cccggatcat tgatacggtg accggccagg ggtgtcgcct
      481 ggcagaccac actaccattt cagcatcagc aagcctgatg aacattgaag aggaggtaat
      541 taaaccggaa ttcggtgccg tgtttggtga tcaggtcact gcaggaccgt tcaccatctt
      601 caaaaactgc atcgttggca ataatgtcgc aattgaaggg gggagccggg tcatctcccg
      661 tatcctgcct gacgaatcta aggtgatata acgtgtgcgg catcgtaggt tatatcggcc
      721 gcagggaagc tgccccgatc attgtcgaag ggctcaagaa actggaatac cgggggtatg
      781 attcgttcgg ggttgccact atcagcgggg gtattgaact ggacaaacac caggggcgga
      841 tatcagattt atcagattcg gcggtttgtc tcaagggaaa aatagggata ggacatacac
      901 gctgggcaac gcatggcgtt cccaatgatg tcaactccca cccccatttg gactgttcag
      961 gtcgtattgc tgtagtacac aacggtatta tcgaaaacta ttcagagctc aaacggcagc
     1021 tgatctcaag gggtcacaca ttcctctctg aaaccgatac agaagtggtg gcacatctca
     1081 tcgaagaggc atatgaacca gaaaaagatc tcctttcagc agtccagaag gtcgtcccca
     1141 aactgaccgg atcctatgcc ctgcttgtaa tcgccacggg tgaagatcgt atcatagccg
     1201 cccgttatgc aagcccgctt gtccttggta tcggtgacgg ggaatttttt gccgcttcag
     1261 atatgacacc agtcattgat cataccgaac gtgcaatttt tttggaagat ggggatgtcg
     1321 ccagtatatc ttatgaaaag gcagagattt tccatgatgg cagaccggtg aaaagaccac
     1381 tggagctgat agactggtcc ggtgaagaca ccaagaaagg cgggttcccg cattatatgc
     1441 tcaaggaaat ctacgagcag ccacaggcat tttataattc ggtcaggtcc gttaccaatg
     1501 aactaatccc tgcaccagtt aaaaacgctg aatcggtaac aatcattgcg tgtggctctt
     1561 cctacaatgc cggacttatc ttcaagtatc ttctcgaaga tacctgcgca atcccggtac
     1621 ggattgattt cgcttccgag ttcaagtatt tccccccacc aatacggggg ctggtaatcg
     1681 gcatctcgca atccggggag actgctgata caatcgcggc acttaagcag gcaaaggtgc
     1741 acaattgcac aacccttgca atcacaaaca tattgggcag ctccatcagc agagcagtag
     1801 attttaccct tttcatgcgt gccggacccg aaatcagcgt ggcagcgacc aagtccttta
     1861 tagcacagct cgctgtcctg atgcaggtca ctaatttcct gcgaaatggc gaatttgagg
     1921 atgtactccg caacggtcac cgggcaatag aagaagtgct cctgattgat ttatcggatg
     1981 cggtcgggct ctgccgtaat gcccggagca tcttctatgt cggaaggggc cctttttatc
     2041 cggtatctct tgaaggatca ctgaaaatga aggagatatc gtatatccac gcagagggtt
     2101 acgctgccgg agagctgaaa cacggaccat ttgccctgct ctcccctgaa acaccggtag
     2161 ttgcaatttg tatgccagga gaaacatacg gggtaatgat ttccaatatc aaggaaatga
     2221 aagcaagggg ggcacccgtt atcgcgattg gggaggaggg ggatctggaa ctttccgagg
     2281 ttgtggatat ctttgttccc ctgccggtga cacatccttt tgtgcaggtg ctgacaagtc
     2341 ttatagtact gcagctcctg gcctactata cggcggttgg tttgaaccgt gatgtagata
     2401 aaccaagaaa cctggcaaag agcgtgactg ttgaatgatt gagtatggca ttaatactgt
     2461 tggaacagga gcacaggttt tcgaacctgt tacccttgga tttccctcaa gggataagat
     2521 gggacagacc ggtttcaccg gcactataat cgggaaaaat gcggtaatcc ggtcaggtac
     2581 cataatctat tgtgatgtaa ccattggaga taattttcag accgggcaca atgtcatgat
     2641 ccgtgaaaaa acaaagattg gccaccgggt cgccattggt acgtctgttg ttattgaagg
     2701 taacacagtt atcggggatg aagtaagcct gcagagcatg gtgtatattc caactgatac
     2761 cattatcggg aataatgtct ttatcgggcc aaatacggta ctcaccaatg accgttaccc
     2821 tccgacacgg attggcggat tgaaagggcc acagatccgt gctggtgcgg caattggtgc
     2881 gaatgcaacg cttcttcccg gtgtctgtat cggtgaggga gcgctggtag cagccggtgc
     2941 aattgtaacc cgagatgtcc cggcacatat gctggcaatc ggtgcacctg caaaaattac
     3001 cgatctgcct caaaagatgg ttcaggaagt tcatcccaaa taataccgaa gtgccattgc
     3061 acttcctcgt ttttttgaat tgtaagccgg atgaaattta ctagggggtg agatattctt
     3121 cagttgttgg gaataccgcc agtttgaggg tttattttgg gattttctcc tgaagaattc
     3181 aaccaaattt attaatggtc ttaacttaaa accaagtacc caagaaaggt tgattacatg
     3241 gtacttattg atcttaaaaa ccaccggacc caactgatta tcggtctggt ttcattattt
     3301 tgtgtctttg ccctatggct tcgtttgctt cccatgctca atatgggtaa cacagacgtc
     3361 ctgatgatgg tggcaagtga tgacccgctg tacaacctgc gacaggttga acagattctg
     3421 gcaaacttcc caagctatgc atggtatgat cctatgacct tgtttcccgt gggaatgtca
     3481 gtcccctggg ggcccctttt ccctaccatc atcgcactct tttgcatcat aaccggcgcc
     3541 accacgcgac cggagatcat aagcacaggt cttctgattc ctcccctgct gggagtcatg
     3601 atcgttgcga tcatgtactt tgtcggaaaa acctgtgggg actggaagac gggtcttctg
     3661 gcatcgggtt ttaccgcaat agtttctggc cagttctttt accgttcatt ttatggatat
     3721 atggatcacc atatcgcaga ggtccttttt tctactatct tctgccttct ctatatgact
     3781 gcagtattct cggaaaaaga cataaaaatc gatctgaaga atattctttc atataaaaag
     3841 accctcctgt tgtcaggtct tgccggcatt gcctatctcc tgggtctgtt tgttatgccg
     3901 acgatgatcc tttttgccat gattgttggc atatttacca ttatccagtt tgttattgat
     3961 gtggcccgtg gcaaaacaag cgaatatctg ctggttatta acaccataat ttttactatt
     4021 gcgattatcg ggcttctcat gaacggatta aaagatcctg ggattaatct tatcttttat
     4081 tcagtcgggc atgtttacgc gtacctggca ttaatcggtg gaacatgttt tctgtacggt
     4141 gttgcacgct tcgttaagga gaaaaactgg tattactatc ctgcagttct tatcgggagc
     4201 ggaattgcat tctctctcat cctccttatc gtcagccctc agttatataa cctgtttatc
     4261 aattcactct tctctttttt cgggcagcag gcaataacca atactgtgca ggaagcccgg
     4321 ggctgggcac ctgatctcgc atggacgaca tttaactatg gtctcatcct gatggtgggc
     4381 ggggcgctgg tcatgctcta caaaaacctt aaggaggaac acccgcacca gatctttgct
     4441 ctcgtgtggt cactcgttat gctggtttcg acctggcagc atatccgcta tgagtattac
     4501 cttgctgtga atgtcgccct gctctcagcc gtgtgtgtca gctttacatt tgaccgggga
     4561 tggccagaaa tccgaaagct ggcatcaagg atttcatccg atacagatgt gcaggaattc
     4621 aataaaggag gtaaggaaga gccacaaaaa ggaaaaaaac agaaaaaaat ccatgggaag
     4681 agctccgtcg caacccagtc gaattttctt gtcatcgtat tcgtaatact taccattgga
     4741 ctcagcattc tgttcgctta cacttctgca tcactcagct atggaaatgc agctcgtgat
     4801 actatacgga tgaatcccga ctggcgggaa acccttgaat ggatgggtaa taatactcca
     4861 gatacaggtg tgaattacac aaccatttac aatccacata cattccagta tcctccagaa
     4921 gcttatgggg tcatgtcctg gtgggactat gggaacatga tcacgtatat tgcaaaacgg
     4981 atccctaacg ccaatccatt ccaggcaggc gttaccggac ccacgggatc agcaaatttc
     5041 ttcatgtccg tatcagaaga tgatggagat aaagtccttg atctcgctaa aacccggtat
     5101 atcatcaccg atatcgaaat ggataccggg aaattctggg ctatggcgac atggtataat
     5161 gcatcggctg gcgcaaatcc ataccagatg acactcctta ctcctgcaca gaatactctc
     5221 aacagttacg agtcagtcct gctcaatacc cggctgtatt accttaccat gatctcgagg
     5281 ctccataact ttgacggctc cctgacaaca cccacgagtg cctattacat agagtacacc
     5341 gatccaacca tttcgcaggt ctctcttccg gtaatcacgg gagcagaagc attaaacgcc
     5401 agtgatgctc tgcttcgtgc agctgaatat aacctcaacg caccggctgg ttatcatgcc
     5461 gcagcattga gtcctgccct cacgctcccg gttgaaacag taccagcact caggcattac
     5521 cggctggtgt atgaatcaaa aagcaatgtg ttcaattcga acacaacaga tgtgaaatat
     5581 gtcaaagtgt tcgagtatgt taaaggggcg cacatccaag gagagggcat tatccaggtc
     5641 ccggtaatca gtaataccgg cagaacgttc atttaccggc aaatgagttc caatggtgaa
     5701 tttattgtcc cctattcaac aaccgggagt acctatgggg taaagacatc cggaaaatac
     5761 aaggtcattt catcgggacg ggaatatgat gtacctgaat cggctgtaat ggaaggagca
     5821 ctcatccgat aatttttttt taagatcaca aaaccaaacc ttttgtttgc agggatctaa
     5881 tagaggatta atactggtat ggtccgttgt tccgcgattt atggggatat tttctctcat
     5941 catgacatgg aaaaccacga tgagagcaat acccggctgg caacagctct tgaaggcgta
     6001 cccgagggag taccgtttat cgcaccgttg tgtgcatctg aaagtgatct tgcaag
//
LOCUS       DASRLV010000012         2994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_896431,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000012 DASRLV010000000
VERSION     DASRLV010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896431"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VFC43_00425"
     CDS             complement(<1..749)
                     /locus_tag="VFC43_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-specific transcriptional regulator TrmB"
                     /protein_id="HZK29690.1"
                     /translation="MSTVLERRREYLRLMRQFTLDKGFFTVTDIQHATSIPRSTAQDW
                     VNRLLIEGCVLVREEKRGRNAAHYVAISAMPSSACRRIFTTIDDDDVEIYHECLSGAC
                     TAFCGYHHALAGGVLNHVERDGTLLKERARIGTREVIVGLSPLPAVGVTGIARDGDTI
                     IQHISCIGGPAYSLSDMMARAEGVLQVSTRQDGPIVEGDVRTLALIHVTIGIDDTDSP
                     EGGATFALALALLSYVTRIKGVLPISHHVAML"
     gene            complement(733..1719)
                     /locus_tag="VFC43_00430"
     CDS             complement(733..1719)
                     /locus_tag="VFC43_00430"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HZK29691.1"
                     /translation="MRVALVGLGSVGRGVAGVIAKKDLGISLTGIADSKSGCMDPDGL
                     DIKNILATKNETGFCGDQKISAQDIISKAEYDVLIEVTPTNAITGEPAIGYIKAALLR
                     KKHVVTSNKGPIALAYKELHMMAQKNGVALRYEATVAGAIPIMHTLEHGLAGNEILAL
                     YGVLNGTCNYILTRMAAEGLTYEQALMEAREMGYAEADPTYDVQGIDAAIKLVILANT
                     IWGNGIRLNDIDRTGIDLLTPDALRLADDQGCTIRLIAEAIPRKHILRVSPRIIEKNH
                     PLVVEGTLNALTLETDMAKEITLIGRGAGSTETASAIIGDLLFIKDRYVHRS"
     gene            complement(1719..2210)
                     /locus_tag="VFC43_00435"
     CDS             complement(1719..2210)
                     /locus_tag="VFC43_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HZK29692.1"
                     /translation="MKLEMKDSPGQLVAALKPISEVGGNIIAVIHQREPDLDDDTLNV
                     QIVLELPKGRLEKLVALLHEQGVRIQRIDEERLLYKRTVIMIGHLMHTDLSDTVDQID
                     STGFAEVCELSMSMPAVNERSSSRITIKAVSKQDMEAALAILRRVSNQKSLLLIEPLE
                     EIA"
     gene            complement(2642..>2994)
                     /locus_tag="VFC43_00440"
     CDS             complement(2642..>2994)
                     /locus_tag="VFC43_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HZK29693.1"
                     /translation="TEQFRADGLLISTPTGSTAYAMSAGGPIVDPRIQGFLLVPLAPY
                     LLSSRPHLISSNRKLEIKMESLKSANLVIDGQKTIGLGTLMTIQVKRSEHPALFVDVK
                     RNFFEKVDSKLRKL"
BASE COUNT          728 a          743 c          721 g          802 t
ORIGIN      
        1 agcattgcaa cgtgatggct gatgggaaga acacctttga tccgggttac gtacgaaaga
       61 agggccagtg caagggcaaa tgttgctcct ccctccggtg aatctgtatc atcaataccg
      121 atggtgacat gtatcagcgc cagcgtccga acatcgcctt ccacaatggg accatcctgg
      181 cgggtactga cctgtaaaac cccttcggct cgtgccatca tgtcggacaa agaataggca
      241 ggaccaccaa tacagctgat atgctggatt atcgtatccc cgtcacgggc aattcccgtg
      301 acaccaacag caggaagcgg tgagagcccc acgataactt cacgcgttcc gatacgggca
      361 cgttctttga gcagggtccc gtcccgttct acatggttca ggactccccc cgccagtgca
      421 tggtgatatc cacaaaaggc tgtgcaggcc ccgctgaggc actcgtgata aatctctaca
      481 tcatcatcat cgatcgtggt aaagatcctg cggcaggcac tcgagggcat ggcactgata
      541 gcaacatagt gtgccgcatt tcgtccccgt ttctcctccc gtacgagaac gcatccctcg
      601 atgaggagcc ggttcaccca gtcctgggca gtactgcggg gaatactggt agcatgctgg
      661 atatcggtga ccgtaaaaaa tcctttatcg agtgtgaact gccgcattag acgcaggtac
      721 tcacgacggc gctcaagaac ggtggacata acggtctttg ataaacagca ggtcaccgat
      781 gatcgcactg gccgtttccg tggaaccggc tcctcttcca atcagggtaa tttcttttgc
      841 catatcagtt tccagcgtaa gcgcattcag tgtcccttca actacaagag ggtgattctt
      901 ttcgataata cggggagaaa ccctgaggat gtgtttcctt gggattgcct cggcaataag
      961 gcggatagtg catccctgat catcggctaa ccgcagtgca tcgggtgtca ggagatcaat
     1021 tccagtacga tcaatatcgt tcaatcgtat cccattaccc cagatagtat ttgcaaggat
     1081 cacaagcttt atagccgcat caattccctg cacatcatag gtcgggtcgg cttcagcata
     1141 tcccatctcg cgggcttcca ttaatgcctg ttcgtaggtg agtccttccg ctgccatacg
     1201 ggtaaggata taattgcatg taccattcag taccccgtac aaagcgagga tctcattgcc
     1261 ggcaagcccg tgttccaggg tgtgcatgat aggaattgct cctgcgaccg ttgcttcgta
     1321 tcgcagtgca acaccattct tttgtgccat catatggagt tctttatagg caagagctat
     1381 cggccccttg tttgaggtaa cgacatgttt ctttcgcagg agggcggctt ttatatatcc
     1441 aatcgcaggt tcaccggtaa tcgcatttgt cggggttact tctataagga catcgtattc
     1501 tgctttactg attatatcct gagccgaaat tttttgatcc ccgcagaatc ccgtttcatt
     1561 cttggttgcc agaatgtttt taatatcgag gccgtcaggg tccatacacc cgctctttga
     1621 atcggcgata cctgtcaggg atataccgag atcctttttt gcaatcacgc cggcaactcc
     1681 cctgccgacg gaacctaacc cgacaagtgc aactcgtatc atgctatctc ctcgagaggt
     1741 tcgataagca gaagggactt ctggtttgag acgcgacgga ggattgcaag agccgcctcc
     1801 atatcctgct tactcactgc ttttatcgtg atccgtgagc tggatcgctc attcacggcc
     1861 ggcatactca tggaaagttc acagacttca gcaaaaccgg tggaatcaat ctggtcaaca
     1921 gtgtctgaga gatccgtgtg cataagatgc ccgatcataa tcactgtccg cttataaaga
     1981 agccgttcct cgtcaattcg ttgtatccgg actccctgtt catggagaag ggctacgagt
     2041 ttttccagac gccctttggg caactcaagt acaatctgga cattcagggt atcatcatca
     2101 agatcgggtt cacgctgatg aattaccgca atgatatttc cacccacttc agaaatgggt
     2161 ttgagggcag caaccagctg accgggagaa tccttcatct cgagtttcat ggaaacctgc
     2221 acacaaccac ctgtcaagta gtagatcggc tttcaagata agccttttat tttgaaaata
     2281 agaaaaaatt ttaaggaact gtttcttgtg caactaatat accggtaatc caaacagagt
     2341 aattgcttca gcaaaatcat gattacgtta ttttaatatt ggctctgtag gattcctatt
     2401 attctagcac aaaataaaaa gaggacacgt gctttgaagg aggggacagt gcctgtcccc
     2461 ccagaccgtc cctgcgcaat gtgatagtgt tgttattacc ttctcggggt ttatcgcatt
     2521 gggggggagc ccaatggcgg tggtgaaacc cccatgaacg ttcgagtttt ttgaatattt
     2581 ctacagggcc tcaatttcct ttttttttaa atggatacgt gaaaattaaa tggtaatttt
     2641 ttcagagctt gcggagtttg ctgtcaactt tttcaaagaa atttcttttc acatcaacaa
     2701 atagtgcggg atgttctgat ctttttacct gtatcgtcat caacgtccct agtcctatag
     2761 ttttctggcc atcaatgaca agattggcgg atttcagact ttccattttg atttcaagtt
     2821 tgcggttact gctgatgaga tgcggcctgg atgagaggag atagggggcg aggggtacga
     2881 gcaaaaagcc ctgtattcgc gggtcgacaa tgggtcctcc cgcactcatt gcatacgcgg
     2941 tggacccggt tggtgtgcta atcaggaggc catctgccct gaactgttcg gtgg
//
LOCUS       DASRLV010000013         5841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_490688,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000013 DASRLV010000000
VERSION     DASRLV010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490688"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VFC43_00445"
     CDS             complement(<1..373)
                     /locus_tag="VFC43_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZK29694.1"
                     /translation="MYAKPLAYWCVPIPDIIEALELKKIFGDLLAVDGISFTVKKGEV
                     FGFLGPNGAGKTSTMKIIACVSPRTSGTVRVFGLDPDLHPAEIKQRLGVVPQETNLDP
                     DFTCFGNLFSYARYFDIPPDSA"
     gene            complement(522..989)
                     /locus_tag="VFC43_00450"
     CDS             complement(522..989)
                     /locus_tag="VFC43_00450"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZK29695.1"
                     /translation="MSVTIADLISDLHNPAIKRRRAAAQHLGRTADPSVIPHLVEALS
                     DTHPMVRSDIVQALGRLGDSSALEALISALEDKDPLVRAATIAALGRIKDRNAVDALI
                     RCLQDREMNIRIGAAEVLGNLKDRRATGPLKKLLDDPFSQVREAAEMAILHIR"
     gene            complement(989..1336)
                     /locus_tag="VFC43_00455"
     CDS             complement(989..1336)
                     /locus_tag="VFC43_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29696.1"
                     /translation="MPRELSEKDITILKKLAPECGDLTCSGSGHTFHSILPPVSNHFS
                     EDSNDFIQRIGRLTEDELIYITDMIRKGEESMGCLPVEDVEALVHLVHDRLSPEIAKK
                     VIAAYESGGECER"
     gene            complement(1358..1498)
                     /locus_tag="VFC43_00460"
     CDS             complement(1358..1498)
                     /locus_tag="VFC43_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29697.1"
                     /translation="MPTCSDCISYSKKTPHTGDCRINGETTPDRESERCPSRTFIPKP
                     VK"
     gene            complement(1557..1916)
                     /locus_tag="VFC43_00465"
     CDS             complement(1557..1916)
                     /locus_tag="VFC43_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29698.1"
                     /translation="MSREKIKVPGKVYEELVALQREIHFTQNHQDTIKKAEDRGYMNA
                     SNWIRQNENAYKIGFAWGFEPMADEPQGTLRDIPARETPAQKRTISRPTPPQKPQKPP
                     ASGGFLAGIKNWLGKYF"
     gene            1977..2273
                     /locus_tag="VFC43_00470"
     CDS             1977..2273
                     /locus_tag="VFC43_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29699.1"
                     /translation="MLRLIDILLQRNGWITKLRYFDTYCEQFINTTEGRSMVNVIYEG
                     DDFKRMMRTDMLKLQRLVDAKKTGIFEVDHKDGKIKVEIKKRGDDIIVKRWRSN"
     gene            2726..3220
                     /gene="sixA"
                     /locus_tag="VFC43_00475"
     CDS             2726..3220
                     /gene="sixA"
                     /locus_tag="VFC43_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HZK29700.1"
                     /translation="MDLFILRHGKAGQSSDNPEDSARKLSKAGRSEIRKVARWMKKKN
                     LRFDCIATSPLARAYQTAEIVAKSLDLKDRLEVWDELVPGGDLDTLCYHASQAGKDAS
                     VILVGHEPGLSDLICRIVTGGDTASLVIAKGGLAKIRNFSFGDKPSGELQWLLTPGHM
                     LDMQ"
     gene            3741..5201
                     /locus_tag="VFC43_00480"
     CDS             3741..5201
                     /locus_tag="VFC43_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29701.1"
                     /translation="MISVFSFTFLVPVASATGIMSDNMDVITVESAVRYVDSPTPDNG
                     GMEAPQDLVSGPSVSGVMIFPADHIWNTRVDSLPVDPRSAAYVSTIGNYSYLHADFGS
                     GLYEGNLIGIPWNIVNGSQAKKTISFAYADESDPGPFPVPDNPLVEGGSDHHLLIIDR
                     DTKMLYELFAAEKQADGSWSAGSGAIFNLSGYSLRPGGWTSADAAGLAILPGLARYDE
                     VNDGEISHALRFTAPTTRQAYLWPARHYASSITDPVYPAMGQRFRLKASFNTSGYPYQ
                     AQIVLQALKKYGMILSDNGAPWYINGAPDDRWNNDALHTLHQLKGSDFEAVDSSSLMI
                     NQDSGQARIQPIFSGTSHHTGVFRNGTWYLDTTKTGTVNRTFHFGTPGDVPVIGDWDG
                     NGVSDTGVFRPANGNWYLDTTKTGTVNRTYHFGTTGDVPVIGDWDGDGVSDTGVFRPA
                     NGNWYLDTTKTGTVNRTFPFGKRGDLPVIGIWQMTN"
     gene            complement(5215..5289)
                     /locus_tag="VFC43_00485"
     tRNA            complement(5215..5289)
                     /locus_tag="VFC43_00485"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5251..5253),aa:Glu,seq:ctc)
     gene            5438..>5841
                     /locus_tag="VFC43_00490"
     CDS             5438..>5841
                     /locus_tag="VFC43_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HZK29702.1"
                     /translation="MSGLEDQIQELEAELTKTPYNKATSKHIGRIKAKIARLRDEAVN
                     RAMKSSGGAEGFSVKKSGDATAVLVGFPSTGKSTLLNKLTGTASAVAAYEFTTLTVVP
                     GALEHKGAKIQLLDIPGLIAGAAMGKGRGKEV"
BASE COUNT         1480 a         1388 c         1515 g         1458 t
ORIGIN      
        1 tcgcactatc ggggggaata tcgaaatacc gggcatagga aaagaggttg ccaaaacagg
       61 taaagtccgg atcgagattt gtctcctgcg ggactacccc gagccgttgt ttgatttcgg
      121 ccgggtggag atccgggtcc aagccaaaga cccggactgt ccctgatgtc ctgggtgaga
      181 cgcacgcgat tatcttcatg gtcgaagtct taccggcgcc gtttggaccc aggaacccga
      241 atacttcgcc ttttttaacg gtgaaactga tcccgtcaac ggcaaggaga tcaccaaaga
      301 ttttctttaa ttccagcgcc tcgatgatat caggaatcgg tacacaccag taagccagtg
      361 gtttggcata tatccataaa aatccccacg cttaaacgca ccaggagaac tctccggaac
      421 aaaccgtctt tgcttttccg gtcgtgtctt tggttagtcg gtgcacggta gagggtaaaa
      481 atgagttgca aatcccttgt tttaaaaaca gtaccacact gttaccggat atgcaggatc
      541 gccatctccg ctgcttcccg gacctgggag aacgggtcat caaggagttt tttcaacggc
      601 ccggttgccc ggcggtcctt aaggttgccc aggacttctg cggcaccgat cctgatgttc
      661 atttccctgt cctggaggca ccgtatcaaa gcatcgactg cattccggtc ttttatcctt
      721 cccagtgcag cgatagtggc agcacggacg aggggatctt tgtcttccag cgcagagatt
      781 aatgcctcaa gagctgagga atccccaagt ctccccaatg cctggactat atcactcctc
      841 accatgggat gggtatcaga caaggcctcc accaggtgcg gtatcactga cgggtcagcg
      901 gtcctgccca agtgttgcgc tgctgcccgt cgtcttttga tggcaggatt atggagatca
      961 ctgatcaggt cagcaatcgt gacggacatc agcgctcaca ttcgcctccc gattcgtatg
     1021 cagcgataac ttttttggca atttccggcg agagacggtc gtggaccagg tgtaccagcg
     1081 cttctacatc ttcgaccgga aggcagccca tgctttcctc tcctttccgg atcatatcgg
     1141 ttatatagat gagttcatcc tccgtaagac ggccgatccg ctgaataaaa tcgttactgt
     1201 cctcagaaaa atggttcgag accgggggaa gtatggaatg gaacgtatgc cccgaacccg
     1261 aacaggtcag gtccccgcac tctggcgcca gtttttttaa aatcgtaatg tccttttcag
     1321 aaagttcccg tggcatgatt ggtggtttcc ttaaaactca tttcaccggc tttggaataa
     1381 acgtacgtga aggacagcgt tcactttccc ggtccggtgt tgtttcacca ttgatacggc
     1441 agtcacccgt gtgcggggtt ttttttgaat aacttatgca gtcagaacaa gttggcatac
     1501 aatcctgtcg gttatgatag gaaataggtg tttttgttga tgaaaaaagg taaaaatcag
     1561 aagtatttac ccagccagtt tttgataccg gctaggaaac ccccggaagc agggggtttc
     1621 tggggctttt ggggtggtgt cggacgcgaa atcgttcgtt tctgtgccgg agtctcgcgg
     1681 gcaggaatat ccctgagtgt tccctgcggt tcatcggcca tgggttcaaa accccaggca
     1741 aagccaatct tataggcatt ctcgttctgg cggatccagt ttgatgcgtt catatatccg
     1801 cggtcttctg ccttcttgat ggtgtcctga tgattctgcg tgaaatggat ctcacgctgg
     1861 agggcgacta actcctcgta tacctttccg gggactttga tcttctccct gctcataact
     1921 cctcatatag cgttagcagg gatccgatat taaatgcttc tcttaaaaaa acggtaatgc
     1981 ttcggttaat tgacatcctg cttcaaagaa atggctggat cacgaaatta cgctactttg
     2041 atacgtattg tgaacaattc atcaacacaa cggagggaag aagcatggtt aacgtgatct
     2101 atgaagggga cgattttaaa cgaatgatga ggacagatat gctgaaactg cagcggctcg
     2161 ttgatgcaaa aaagacagga atttttgaag tcgaccataa agacggaaag ataaaagtgg
     2221 agataaaaaa gcggggagat gacattatcg tgaagcggtg gcgttcaaac tgagatcacg
     2281 gttaccgtaa aatgttttgt atatgaggtt tatcaggcaa cgaatttcct gcgtctcctt
     2341 aaagattgat gacggggaaa tcatttaaaa aatgacgggt aacgggagac ttccgggtta
     2401 ccgtgcacag gctttgacaa aatcccgtgc gataataatc ccgccgatgt gttttttttc
     2461 attcagtgaa gccgcctctc atgctgtgca gccataatct ttgccgcttt ttggatgctg
     2521 atcccagcct ccgctgtgta taggggatga ggatgcgcta ccgaaggccc atcaagggat
     2581 ttgtccggaa caaggaattt tgagaagagg tcttcgtaca gtaaatatcc cccagggctt
     2641 ttccctgctg ttcatgatac cgctccctac ccgtttttcc ccatgatgca agagaataaa
     2701 tacgaatgac tgagagtgcg tttgtatgga tttgtttatt cttcgacatg ggaaggccgg
     2761 gcaatcctca gataatcctg aagatagtgc ccgcaaactc tcaaaagcag ggagatctga
     2821 gataagaaaa gtggcccggt ggatgaagaa aaaaaatctc cggttcgatt gcattgccac
     2881 aagccctctt gcccgtgctt accagaccgc agagattgtg gcaaaatcgc ttgacctcaa
     2941 agaccgcctg gaggtctggg atgaacttgt accgggtggc gacctggata ccctctgtta
     3001 tcatgcttct caggctggaa aagatgcgtc tgtaatcctt gtcggacacg aaccaggttt
     3061 atccgatctc atctgcagga tagttaccgg tggggacaca gcatccctgg tcatcgccaa
     3121 agggggtctg gccaaaatcc ggaacttttc ctttggtgac aagccgtctg gtgaactcca
     3181 gtggttactg acaccaggac acatgctgga catgcaataa gacgttgata gcggtgacag
     3241 gcttcagcaa cccggagact ggcagcaaaa aaaaggcaac agacgccggg gcattgaagg
     3301 attacgatgt tgtcagattg atactacccg gaatccatgg ataatagcat tatcttttca
     3361 gcatgtgcct ttaattataa actcaccggt agtttattat tataaaatct gatgtgaatg
     3421 aggagaaaag accgctatga aacccctgaa gtgttttaac aaaatgtaat cccgaaaatt
     3481 tttttatagt acaaaagccg gagccctgat acgcttttca atgactgaag tgcaaaaggc
     3541 ttcatcactc aatggaattc aacagggcct gaatttcaat ctgttttatg gtatcacgct
     3601 ggcattggat cctgaaccat caggttatag ttgcccaata tcgggttatg ggtaatttat
     3661 atacccgctt ggagaagatt tttgcaggta aagcaggatg aagaaggttt tttaaattga
     3721 acacctggat aaggatcgtc gtgatttccg tattttcatt cactttcctg gttcccgtcg
     3781 ccagtgcgac aggtatcatg tctgacaata tggatgtaat taccgtagaa agtgcggtcc
     3841 ggtatgttga tagtcccact cctgataacg gtggtatgga agcaccgcag gaccttgtca
     3901 gcggaccatc agtcagcgga gttatgatct ttcctgccga ccacatctgg aatacccgtg
     3961 ttgattcact tcccgttgat ccacggtctg cagcatatgt cagcactatc ggaaactatt
     4021 cctacctcca tgctgacttc ggctcaggct tatatgaagg caacctgatc ggcattccct
     4081 ggaatatcgt gaacgggtcg caggcaaaga aaaccatctc ttttgcctat gcagatgaaa
     4141 gtgatccggg tcccttccct gttccggaca acccgttagt ggagggtggg tcggaccatc
     4201 acctgctgat tattgacagg gacacaaaga tgctctatga actcttcgcc gcggagaagc
     4261 aggctgacgg atcatggtcc gccggctcag gggcgatttt taatttgtcc ggttattcgc
     4321 tgcgacccgg tggctggact tctgcggatg cggcgggtct tgccattctg cctggtctgg
     4381 cccgctatga tgaagtcaat gatggtgaga tttcccatgc actccgtttc acggccccga
     4441 caaccaggca ggcctacctg tggcctgccc gtcattatgc ctcatcgatc acggatccgg
     4501 tatatcccgc gatgggtcag cgattcaggc tcaaagcatc tttcaatacg tcaggatacc
     4561 cttaccaggc acaaattgtg ctgcaggcac tgaaaaaata tgggatgatc ctgtccgata
     4621 atggtgcacc gtggtatatt aacggtgccc cggatgatcg gtggaacaac gatgccctgc
     4681 atactcttca ccagctgaag ggatcggatt tcgaggcagt ggacagcagt tcgctgatga
     4741 ttaaccagga ctccggacaa gcccggatac aaccgatatt cagcggtaca tctcatcaca
     4801 ccggcgtatt ccggaatgga acctggtacc tggatacgac aaagaccgga acagtcaacc
     4861 ggacattcca tttcgggaca ccgggagatg tcccggtaat tggagactgg gatgggaacg
     4921 gcgtatcgga tacgggggta ttccgtcctg caaacgggaa ctggtacctg gatacgacaa
     4981 agaccggaac agtcaaccgg acataccatt tcgggacgac gggagatgtc ccggtgattg
     5041 gggactggga tggtgacggc gtatcggata cgggggtatt ccgtcctgca aacgggaact
     5101 ggtacctgga tacgacaaag accggaacag tcaacaggac gttccctttt gggaaaaggg
     5161 gggatttacc agtgatcggt atatggcaga tgactaatta gagaacagga acagtgctgc
     5221 tatggggatt cgaaccccag tcgcgagcgt gagaggctcg catgattggc cggactacac
     5281 tatagcagcg taattgctca ataacatagg cgatattttt acttaaacgt gttgataacg
     5341 ggaactaccg tcctgattcc cttaccttgg tgaggttatc acacatccct cgaaccataa
     5401 acacctttat ctcggacgtg cagcgattaa tataagaatg agtggtcttg aagatcagat
     5461 ccaggagctg gaagcagagc ttaccaagac tccgtataac aaggctacat ccaagcatat
     5521 cggccggata aaggcaaaga ttgcacgtct ccgcgatgaa gcagtcaacc gtgcgatgaa
     5581 atccagcggt ggcgcggaag gcttttctgt caagaagtcc ggcgatgcca cggctgtgct
     5641 cgttggtttc ccctcaacgg gtaaaagtac gctgctcaac aaactcaccg gcacggcaag
     5701 tgcagtagct gcgtatgagt ttactacact cacggttgtc cctggtgccc tggagcacaa
     5761 aggggccaag atccagctgc ttgatattcc gggacttatc gccggggctg caatgggaaa
     5821 aggaaggggc aaggaagtga t
//
LOCUS       DASRLV010000014         2953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_167948,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000014 DASRLV010000000
VERSION     DASRLV010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167948"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VFC43_00495"
     CDS             complement(<1..339)
                     /locus_tag="VFC43_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HZK29703.1"
                     /translation="MKILFVVCGEGLGHASRCLHLGRYLHKQGHDIHFASYGRSLDFL
                     FKQEPLNLHRTRREVCLEGEMGFFSLKKTLWCSKWIVLDMVLSVLRISQLIRKHRFAC
                     VVSDTMYGGVL"
     gene            complement(370..600)
                     /locus_tag="VFC43_00500"
     CDS             complement(370..600)
                     /locus_tag="VFC43_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HZK29704.1"
                     /translation="MNRLYRSTKDRMLGGVCAGLGEHLDVDPTVIRLVWVVLTAVSMG
                     TGILVYILAWIIIPEEETPGGTPPSSGPGEIT"
     gene            706..1197
                     /gene="msrA"
                     /locus_tag="VFC43_00505"
     CDS             706..1197
                     /gene="msrA"
                     /locus_tag="VFC43_00505"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HZK29705.1"
                     /translation="MNVDADRYKKATFGAGCFWGVEAAFSVVPGVISTDVGYMGGTLK
                     NPTYENVCTGTTGHAEVVQVTYDPKKVSYGQLLDIFWSVHDPTQLNRQGPDIGTNYRS
                     VIFFHDAEQGKIARKSKESLEVSGKHGFGRIMTTILPAPEFYRAEEYHQQYHKKHGGS
                     CRF"
     gene            1250..1882
                     /locus_tag="VFC43_00510"
     CDS             1250..1882
                     /locus_tag="VFC43_00510"
                     /inference="COORDINATES: protein motif:HMM:NF014028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29706.1"
                     /translation="MIKQNKKESTTKTGCIRIPPSALWVKTFCVFLVLVSLVMSPGCT
                     TAPSVPGDTTTVTPSHSTTVSPLPSASSPASDTGQTAANIQLKGNVYGISTNPQVGIE
                     IITFTIGLAQQAPADIDLSRLEIAFLAPGSDTVILKRGTRDSTSMFTATMGNNEVTSL
                     HPGNEVEIVFRVKGVPAGSRVNIELKPSSGAALPISRTVPAMLSSVNNLG"
     gene            complement(2204..2374)
                     /locus_tag="VFC43_00515"
     CDS             complement(2204..2374)
                     /locus_tag="VFC43_00515"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HZK29707.1"
                     /translation="MMSLNCQDIGMSESFELTGTNEREVMRNFISYAETELKMPFLTA
                     DTIYRVQMAIKK"
     gene            complement(2686..>2953)
                     /locus_tag="VFC43_00520"
     CDS             complement(2686..>2953)
                     /locus_tag="VFC43_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29708.1"
                     /translation="SIREGVRFFAILTTIRTMGWLSYILALIVFIVVAVIYAIIAGIL
                     SIIPFIGWVLVLIIAPFFSIFIARYFTLVYDQGEPQAGPQAPIE"
BASE COUNT          825 a          683 c          724 g          721 t
ORIGIN      
        1 aagcacacct ccatacatgg tatcactcac cacacaggca aaacggtgtt ttcggataag
       61 ctggctgatc ctcaggaccg agaggaccat gtccagaact atccatttgg agcaccagag
      121 tgtttttttc aggctgaaga accccatctc cccttccagg cagacttccc gtcgtgtccg
      181 gtgcaggttg agaggctcct gcttaaacag gaaatccaat gacctgccat agctggcaaa
      241 atggatgtcg tgtccctgtt tgtggagata tcggccaaga tggagacacc gggatgcatg
      301 gccgagtccc tcaccgcaga caacaaacag gatcttcatg gccgttccca aaacaaatgc
      361 tgagcttttt taagtaattt ctccgggtcc tgatgacggg ggggtcccac ctggtgtttc
      421 ttcttcgggg ataataatcc aggccaggat atacacaagt atgcccgtac ccattgagac
      481 agccgtcagg actacccaga caagccggat gaccgtgggg tcaacatcga ggtgttcacc
      541 aagaccggca cagactccgc cgagcatcct gtctttagtt gagcggtaca aacgattcat
      601 gttagaaata ttgctttttc acccttaaag gtacgcgcat aaagtgactg gcaccataga
      661 agggtctaat aggtttgagg gatgtatggc ataggaggaa agtccatgaa cgtggatgcg
      721 gacaggtata aaaaagcaac atttggtgct ggttgtttct ggggcgttga agcagccttc
      781 agtgttgttc cgggagttat ttcaacggac gtcgggtata tgggaggcac actgaaaaat
      841 ccgacttacg aaaacgtctg tacaggcaca acgggacatg cagaggttgt gcaggtaacg
      901 tatgatccaa aaaaagtcag ctacggacag cttcttgata ttttctggtc ggtccatgat
      961 cccacacagc tgaaccggca gggtcccgat ataggcacaa attaccgatc ggtaattttc
     1021 ttccacgatg cagaacaggg aaaaattgcc aggaaatcaa aagagagcct tgaagtttct
     1081 ggaaaacacg ggtttggcag gattatgacc actatcctgc cggccccgga attttatcgg
     1141 gcagaagaat accaccagca gtatcacaaa aagcatgggg ggtcgtgcag gttttagtca
     1201 ccccggtaat aaataccgtg tgttaaaata ccggaatttt gtgatgaaca tgattaaaca
     1261 aaacaaaaaa gaatcaacta ccaaaacagg atgtatcagg atacccccat cagcgttatg
     1321 ggtaaaaacc ttctgtgttt ttcttgtact ggtttcattg gtcatgagtc cgggttgtac
     1381 tacagctccc tcagttcctg gcgatacaac aacagtaacc ccctcgcatt caacaacagt
     1441 gtcgcctctg ccttctgcat cctccccggc atcagatacc ggacaaactg ctgcaaacat
     1501 ccagttgaaa gggaatgtgt atgggatatc tacaaatccg caggtgggta tcgaaataat
     1561 tacttttact atcggtcttg cacagcaggc accggctgac atagacctga gccggctgga
     1621 aattgccttt ttggccccgg ggtctgatac ggtaatcctc aaacggggca caagagattc
     1681 aaccagcatg tttaccgcta ccatgggtaa caacgaggtc acttcactgc accccggtaa
     1741 tgaagttgag atcgttttca gagtgaaggg tgttccggcg ggtagcagag tgaatattga
     1801 gttgaaaccg tcttctggtg ctgcattgcc catctcaagg actgtgcctg caatgctgtc
     1861 atcggtgaat aatctcgggt gaactacttt tttacgttat aaaattttaa aaaaacatac
     1921 agtctactgt gaaaaatcgc aataatgata ggagtgacgc ttcgctacaa gtgaatcacc
     1981 cgggcggtgt tcatggttcc cggtcaaaga gcccgtcctc ccatcaggga ggttatcata
     2041 aaaatgtata catatcacct ggatcgggct ctctggttgg tgacaaaaaa atcagtccgt
     2101 ggtgtattat taaaaaaaaa caggagttct taaaaaaagc gtctgcggaa aggacagaca
     2161 gatccgaata tgacaatccg ggggtttata aagttcagtc cccttatttt tttatcgcca
     2221 tctgaacccg ataaatggta tcagcggtca ggaatggcat tttgagttcg gtttctgcat
     2281 aactgataaa atttctcatc acttctctct catttgtccc ggtcagttcg aatgactcgc
     2341 tcattccgat atcctggcag tttaatgaca tcatgttgaa caccttcctc tttgttaaca
     2401 ccactcttca tgagaaaata ataaagactg gcgaggcagc gttgcaaggc gctgctgtct
     2461 tgcagtgtgc cgattttcac aaattcacga gagagactca atgtccggac tgactggacc
     2521 ggcagttggc aatgcgtctt ttatccttta cggagttctc tgaccatcca caggatagct
     2581 gccatattca gtaagataag gctcgttgat gagcagggcg aaagtgaact tttaccagga
     2641 agggattaat ccggaaatcc ccctgcaaac aaaaaaggga tttggttatt ctattggtgc
     2701 ctggggccct gcctgcggct cgccctgatc gtatacaagc gtgaagtacc gggcgatgaa
     2761 gattgagaaa aacggggcta tgatgaggac gagaacccag ccgatgaacg ggataattga
     2821 caggataccg gcaattatgg catagatgac cgcgacaaca ataaacacga taagggcaag
     2881 gatatacgac agccatccca tcgtccggat ggtcgtaagg atcgcaaaaa accggacccc
     2941 ttcgcggatg ctg
//
LOCUS       DASRLV010000015         3343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_247330,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000015 DASRLV010000000
VERSION     DASRLV010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247330"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VFC43_00525"
     CDS             <1..312
                     /locus_tag="VFC43_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZK29709.1"
                     /translation="TKEQKDLATAYLKPLMMATENTSACFCDLMTKVINQGLCMGCGS
                     CASACPVNAITHEYGKPQGERDLCIKCGACYNQCPRSWFSFDVVENYEAINEAIMAAL
                     Q"
     gene            324..1250
                     /gene="frhB"
                     /locus_tag="VFC43_00530"
     CDS             324..1250
                     /gene="frhB"
                     /locus_tag="VFC43_00530"
                     /EC_number="1.12.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase subunit beta"
                     /protein_id="HZK29710.1"
                     /translation="MGAELGKYKSCVSARSTDKEILKHAQDGGIVTQLFGFALDEGII
                     DGAIVAATKEFAHKHPEKAMRDNSNMEFNEPWRPIPCVVTTKADMIAAAGTKYNISPN
                     VSLIKEATRSFGLDKVGIVGTPCQMQAIRKLQLYPVGARDVGASIALALGIFCMENFP
                     YQSILQLVEDHAGMHMDAVKKMEIGKGKFWVYGKRGQVVQLPLKVTHKYEQPGCHVCL
                     DYVANLGDISTGSVGSPDGWSTVFVRSKIGDSVWAKAMAAGVFETQPIDKVKPGLELV
                     SKLANEKISKNKKTLEERATFGVGKGLRNPYI"
     gene            2074..2559
                     /locus_tag="VFC43_00535"
     CDS             2074..2559
                     /locus_tag="VFC43_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2124 domain-containing protein"
                     /protein_id="HZK29711.1"
                     /translation="MTTKEVFHGVPGILRPFKEYIENGLKKGDQVVYYGVPGTCTPFV
                     ELLGFAVRSLELEQVFVPLVDESKAHKIHNVPEIGMQAGAVLASVKPKAIVIMGGLSM
                     PNVPIKAEQVKAVIDRHPGALVIGVCFMHMFEKAGWLTTVSFDCMIDANIDPVEVTKN
                     T"
     gene            2771..>3343
                     /locus_tag="VFC43_00540"
     CDS             2771..>3343
                     /locus_tag="VFC43_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HZK29712.1"
                     /translation="MEKGFFFDNGKTRIINDNIFSTKKVAAGSIDLLITSPPYNVDIK
                     YSSHNDQVTYKEYRNFSLRWMKRCFNWLRDDGRFCLNIPLDKNKGGQQSVGADLTTIA
                     KKIGFSYHSTIIWNEGNISRRTAWGSWKSASAPYVIAPVELIVVLYKKSWKKASGSGV
                     SDINRKEFMDWTNGLWTFNGERKKKTGHPAP"
BASE COUNT          929 a          799 c          801 g          814 t
ORIGIN      
        1 accaaggaac agaaggacct tgcgactgca tacctgaagc ccctcatgat ggcaacggag
       61 aacacaagcg cctgcttctg tgacctgatg accaaggtca tcaaccaggg tctctgcatg
      121 ggatgcggca gctgtgcatc agcatgcccg gtcaatgcca ttacccacga gtacggcaag
      181 ccccagggcg agcgcgacct gtgcatcaag tgcggtgcgt gctacaacca gtgcccgcgg
      241 agctggttca gcttcgatgt tgttgagaac tatgaggcga tcaacgaggc cataatggcg
      301 gcactccagt gaaggtgatt gaaatgggag cagaactcgg aaaatacaaa tcatgtgtct
      361 cagcacggag caccgacaag gagatcctca agcacgccca ggatggcggt atcgtcaccc
      421 agctgtttgg attcgctctg gatgagggta tcattgacgg cgcaattgtc gcggcaacca
      481 aggagtttgc gcataagcat cctgaaaaag ccatgaggga caactcgaac atggagttca
      541 atgaaccatg gagaccaatt ccgtgcgttg tcacaacaaa ggctgatatg attgcagcag
      601 ctggtaccaa gtataacatc agcccgaatg tttccttaat caaggaagca acccgcagct
      661 tcggccttga caaggttggt attgtcggaa ccccctgcca gatgcaggca atccggaaac
      721 tccagctcta cccggtcggt gcccgtgatg tcggagccag catcgctctt gcactcggaa
      781 tcttctgcat ggaaaacttc ccctaccagt ccattctcca gctcgtggaa gaccacgcag
      841 gcatgcacat ggacgcggtc aagaagatgg agatcggcaa aggtaagttc tgggtctacg
      901 gcaagcgcgg acaggtcgtc cagctgccgc tcaaggtgac ccacaagtac gagcagcccg
      961 gctgccacgt ctgtcttgac tacgttgcaa acctcggtga tatctccacc ggatcagttg
     1021 gcagccccga tggatggagc accgtctttg tcaggtcaaa gatcggggac tctgtctggg
     1081 caaaggcaat ggcagcaggt gtcttcgaga cccagccgat cgataaggtt aaaccaggtc
     1141 ttgaactcgt ttccaaactg gcaaacgaga agatctcaaa gaacaaaaag acgctcgaag
     1201 agcgtgcaac cttcggtgtg ggcaagggtc tccgcaaccc ctacatctaa attttttttg
     1261 gttttctatc agatcgactt attgatgttg atcgaaaata ttgcagactg gcagttcaca
     1321 ttttttataa caaaaacaca ggaaaatttt agcggaaata gtaaagcagg tcttcttcca
     1381 ttttaccata cttctgccta tctgaaaaga gatcctccat ccaaatagga ataaacaggc
     1441 cggtttttct cctcttgaat agggttattc cgaattctac gcccgtatat caattatctg
     1501 aaaaaatgtg atcaaaagtg ctccaataag gccggttacc gggaccttgt gaaaaaaaga
     1561 ttatacctgc attatgcccc gattatcatt ttaggacatt ttttggatct atatgaggct
     1621 ccgctcggtt acggtcatct gatgtccgtt atctgatcat cacccaaaac cacagaaaaa
     1681 ccagagttca aattccctaa aaatcccgga ataatgcaac cccctgagtc ccagaatcgc
     1741 ccaattcagc ctccgttttt cctcccggtt ttcagttcga taccgaaaaa cgccgtaaaa
     1801 tcctttgctt ctgtccgggt ggagaatacg cctaattccc accctgtttg ccaaaataag
     1861 gcagaaatgc acctcatcaa aagggcacca gtggtcgttt aatcgaggca ttttttgcca
     1921 catgtattat gccatcagcc cccataaaag ccgccggaac accaatatga ggttcccttt
     1981 tttccgacgt gataattttc ttgtttcaac aattaacggt tgccggataa taaagtaatt
     2041 attatcatct atccatggaa gtgtagatct gtaatgacaa caaaagaggt ttttcatggt
     2101 gtcccgggaa tccttcgccc attcaaggag tatatcgaga atggattgaa aaaaggcgat
     2161 caggtcgtct attatggtgt gccaggtacg tgcaccccgt ttgtcgagct gctcgggttt
     2221 gccgtccgct cgcttgaact tgaacaggtc tttgttcccc tggtagatga gagcaaggca
     2281 cacaagatcc ataatgttcc cgaaattggg atgcaggccg gtgctgttct cgcgagtgtt
     2341 aagccaaaag ctatcgtcat catgggagga ctctccatgc ccaacgtacc gattaaagca
     2401 gagcaggtga aagcggtgat tgaccggcac ccgggcgcac tggtaatcgg ggtctgtttc
     2461 atgcatatgt ttgaaaaagc cgggtggctt acaacagtat cgtttgactg catgattgat
     2521 gcgaatattg atccggttga agtgacaaaa aacacctgat gccagtccgc gatgtcagat
     2581 tagaaggaaa tccgtacatt tgccaaaaca tacgcttgtt ttaaagcgat tatggtaatt
     2641 ctatcctgtc tgtacttgtc ttaccttaaa aaattcaaca caacagaaca cgaattccaa
     2701 aaaggtctgc tcttccctcc aagcgtaatt ctatagcaaa gaataacgaa attgtacaca
     2761 ctggtgcagc gtggaaaaag gctttttttt tgataacggg aagacccgga ttatcaatga
     2821 taatattttt tcgacaaaaa aggtcgctgc cggtagtatt gatttgctta tcacttctcc
     2881 gccatataac gtcgatatca agtacagttc ccataatgat caggtgactt ataaggaata
     2941 ccgaaatttt tccctgcgtt ggatgaaacg gtgctttaac tggctcagag atgacgggag
     3001 attttgcctg aatatacccc ttgacaagaa caaaggcggc cagcaaagtg tcggggcgga
     3061 ccttactacc attgcaaaaa aaattggttt ttcctatcat tcgaccatta tctggaacga
     3121 gggaaacatt tcacgccgga ccgcatgggg atcctggaag agcgcatcgg ctccgtatgt
     3181 tattgccccg gtggagctca ttgtggtgct ctataaaaaa tcatggaaga aggcgagcgg
     3241 ttcaggagtg tcggatataa accggaaaga gttcatggac tggactaacg ggctatggac
     3301 gtttaacggt gaaaggaaga aaaaaaccgg tcatcctgca cca
//
LOCUS       DASRLV010000016         2655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_410038,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000016 DASRLV010000000
VERSION     DASRLV010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410038"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="VFC43_00545"
     CDS             complement(<1..1084)
                     /locus_tag="VFC43_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HZK29713.1"
                     /translation="MSGIPRIMIAGTHSGCGKTTIASGIMAALTARGYKVQPFKVGPD
                     FIDPSHHTRICGRPSRNLDPFMMKEEGCIDTFFRSTRGADIAVIEGVMGIYDGIDGSD
                     FASTAHVARILDTPVILVVDVKGMSRSVLALIKGYQDFDTTISFAGVILNRVGSPRHR
                     RMIEGSLSLPSLGWIPKREDIAVESRHLGLRMANETGLMGEFGKVIEEFCDLDAIIAI
                     SDNSPGLIPQEKIVLPGKKKRIRATIGVALDDAFCFYYQDNLDHLRACGAEIVFFSPL
                     TDPLPDVNALYIGGGYPELHLPELESSACTGEIKSAVDAGMPVYGECGGLLYLTREIR
                     SDRAYRLCGVLPAESEMTSHVQALGYV"
     gene            complement(1081..2283)
                     /locus_tag="VFC43_00550"
     CDS             complement(1081..2283)
                     /locus_tag="VFC43_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZK29714.1"
                     /translation="MSSIILEARDVRYRYPRAMEAICGISFHIRKKEKIALVGPNGAG
                     KSTLLKMFNGMIRPDSGLMLFDNQPIGYDTASLRLLRKRVGFVLQNPDRQIIAPTVYQ
                     DVAFGPTNLGYPENEVKRVVALALRQVGLDGFERRPPHQLSGGEKKRVAIAGVLAMDP
                     DVLVLDEPTSGLDPSGSEDIMELLDELNHEGKTIIISTHNVELAYPWAERAILMLEGK
                     ILEEDIPEKAFGNPEHVRRAHLSLPTLLELHIELQKRGFPLQGKKPHTVLDMMHCIET
                     MFEKTARHTKPGTITICNVDRDNRESLSTWISSRPGTPVGAMGTRAKQRAEISQIRLD
                     FTYGVIDKCILKALVGENSLIMTTDSMVQRVNQRVDAYCRESGIRIAINQLNPVPESA
                     DEVNGSLK"
     gene            complement(2294..>2655)
                     /locus_tag="VFC43_00555"
     CDS             complement(2294..>2655)
                     /locus_tag="VFC43_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HZK29715.1"
                     /translation="LRRCKVPETIIDLMMIIYRTIFILMDQVIQIYHAQVMRLGYSTW
                     RESLYSFASLCGAAFIASWDAGEDLIRAMDARCYDGKFALLGESRPVEKLPCLAVGIF
                     LVLSTAVVIVSGNSTII"
BASE COUNT          649 a          694 c          625 g          687 t
ORIGIN      
        1 gtacataacc aagagcctgg acatgcgacg tcatttctga ttcggccgga agcacaccac
       61 ataaccggta tgccctgtca gacctgattt cgcgtgtaag atacaggagc ccaccacatt
      121 caccatatac cggcattcct gcatcaacag cagattttat ttccccggta catgccgacg
      181 attccagttc aggcaggtgc agttccggat accctccccc gatgtagagg gcattgacat
      241 ccggcaatgg atctgtcaat ggactgaaaa agacgatctc tgcgccacag gctcgtaagt
      301 gatccaggtt atcctgatag taaaagcaga atgcatcatc cagtgctaca ccaatagtgg
      361 cacggatacg tttctttttt cccggcagga caatcttttc ttgtggaatt aaccccggac
      421 tattatctga gattgcgata attgcatcga gatcgcagaa ttcctcgatt acctttccaa
      481 attctcccat taatcctgtt tcatttgcca tcctgagacc aagatgacga ctttcaaccg
      541 caatatcttc acgtttaggt atccagccta aagatggcaa tgaaagggac ccctcgatca
      601 tccgccggtg tcgcggactt cccacgcgat tgaggatcac ccctgcaaaa gagattgttg
      661 tatcaaagtc ctgatatccc tttatgagtg ccagcacact gcgggacatc ccttttacgt
      721 ccacgacaag gatgaccggg gtatccaaga tgcgtgccac atgggcggta ctcgcaaaat
      781 cagatccatc aataccgtca tatatcccca tcacaccctc gataaccgcg atatcagccc
      841 ccctggttga gcggaaaaat gtgtcaatgc aaccctcttc tttcatcatg aagggatcaa
      901 ggttccgtga agggcgaccg cagatccggg tgtgatggga agggtcgata aaatccggtc
      961 ctactttgaa gggctggact ttgtaacccc gtgcggtcag tgcagccatg ataccgcttg
     1021 cgatggtagt ctttccacac ccgctgtgcg tcccggctat catgatccgg ggtataccgc
     1081 tcatttcaga ctcccattca cttcgtcagc ggattcagga acaggattga gctggtttat
     1141 ggcaatacgg attccacttt cacggcaata ggcatcaaca cgctggttaa cgcgttgaac
     1201 catgctgtct gtagtcataa tgagagagtt ttctcctacc agggccttaa ggatgcattt
     1261 gtctattaca ccataggtaa aatcaagccg gatttgggaa atttccgccc gctgttttgc
     1321 ccttgtcccc attgcaccaa caggagtacc aggccgggag gatatccatg tcgataaact
     1381 ttcccggttg tcccgatcaa cattgcagat tgttattgtc ccgggctttg tatgacgggc
     1441 ggttttttca aacatggtct cgatacagtg catcatgtca agtacggtgt ggggtttttt
     1501 tccctggaga ggaaaacctc gtttctgtag ttctatatgc aattcaagaa gggtcgggag
     1561 ggacaggtgc gccctgcgca cgtgttccgg attaccaaat gctttttcgg gaatatcttc
     1621 ttcaaggatc tttccctcca gcatcagtat tgcacgttcg gcccatggat atgccagttc
     1681 aacattgtgc gtggagatga tgatggtctt gccttcatgg ttcagttcat cgaggagttc
     1741 catgatatct tctgatccgg atggatcgag cccgcttgtt ggttcatcaa ggacaagcac
     1801 atccggatcc attgccagga ccccggctat agcaacccgt tttttctctc caccggaaag
     1861 ctggtgggga ggccggcgtt caaaaccatc cagtcctacc tgcctgagtg ccagtgccac
     1921 aactcttttt acttcatttt cagggtaccc gagatttgtt ggtccgaatg ccacatcctg
     1981 gtacacggtc ggggcaataa tctggcggtc agggttctga aggacaaatc ccacacgttt
     2041 gcgcaacaac cgtaaagatg cggtatcata cccgattggc tggttgtcaa agagcatgag
     2101 cccggagtct ggacggatca ttccattgaa catcttcagg agcgtggatt ttccggcgcc
     2161 gttgggtcct accagagcaa ttttttcctt cttccgtata tggaaactga taccgcagat
     2221 cgcctccatt gcccgtggat agcgatagcg gacatcccgt gcttcaagta tgatgctgct
     2281 catggttccc gacctagatt attgtactgt ttcctgatac aattacaact gcagttgaga
     2341 gcacaagaaa gattcccact gcaagacaag gcagtttctc aaccggacgg gactctccca
     2401 aaagtgcaaa tttcccatca taacatcgtg catccatcgc ccgtatcagg tcctctccgg
     2461 catcccagct ggcaataaat gcggcgccgc agagggacgc aaaagagtat aatgattcgc
     2521 gccaggttga atatccaagg cgcatgacct gagcatggta tatctggata acctggtcca
     2581 tcagtatgaa tatggtacga taaatgatca tcattaagtc aattattgtt tccggaacct
     2641 tgcaccgccg taaaa
//
LOCUS       DASRLV010000017         8426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_491128,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000017 DASRLV010000000
VERSION     DASRLV010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8426
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491128"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..974
                     /locus_tag="VFC43_00560"
     CDS             186..974
                     /locus_tag="VFC43_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HZK29716.1"
                     /translation="MMQKRNKRPRMKGTPPAPKKFSSDPVFSVCWEGPLPEPSVRTAE
                     DLRGVLADPGCTSTGPVYYMYRDVARSVKDRSWLTRQQLRFDITVIPPREICGEFIKT
                     KGHYHPDNPSGTGYPEIYEVLAGEAHYLIQDMYCSDVVMIAACAGEIVVVPPGYGHVT
                     INPARTTVLQMANIVSSQFSSNYQRYEAQRGAAYFEMVKERFVQNPAYAKPPPLRCVK
                     AQRLAGVRNTITAPLYDLIEKRAPVLEFLNYPENYASLFRELCP"
     gene            complement(1451..2950)
                     /gene="malQ"
                     /locus_tag="VFC43_00565"
     CDS             complement(1451..2950)
                     /gene="malQ"
                     /locus_tag="VFC43_00565"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HZK29717.1"
                     /translation="MKQRGSGILLHITSLFSGYGIGDLGPSACQFVDFLSDAKQRYWQ
                     ILPIHPTSPKYDNSPYHSISTFACNTNLISPDCMIKDGFLHEHDISGVPAFPRGIVDF
                     DAAIRFKEQLFSLAYERFRQDQTGRTGYDEFCRNNSRWLEDFALFTSLHRHYTGSSWS
                     TWPEAIKYRDPAALVAMRQTLQKNIEREKFLQYMFFRQWQALRDYCIKKGIRLIGDIP
                     MYVSYDSADVWTHPELFRLDDNLLPLVIAGVPPDYFSKTGQLWNNPLYCWEEHERSGF
                     SWWIDRIGHTLSLVDYTRIDHFRGLVAYWEVPAGADNAITGKWVPAPGEKLLTVLKNR
                     VVDLPLIAEDLGIITPDVSDLMDRFDLPGMRVLLFAFTGDPSGSPHAPHNLKKNCILY
                     TGTHDNAPVRGWLESEATTGEKHQLFRYIGQELPAEQVPAALIRLAMMSVADTVLFPM
                     QDILGIGAQSRMNRPGTDAGNWRWQLEKDQFSPLLTENLGDMTEVYGRT"
     gene            complement(3021..5516)
                     /locus_tag="VFC43_00570"
     CDS             complement(3021..5516)
                     /locus_tag="VFC43_00570"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HZK29718.1"
                     /translation="MSRFVCIHGHFYQPPRENPWLEEVEIEDSAYPYHDWNERIAAEC
                     YAPNAASRILDSDKRIIDIVNNYSRISFNFGPTLLTWLEHNQPDVYSAILKADKDSIN
                     RFSGHGSAIAQVYNHIIMPLASHRDKRTQVVWGIQDFIFRFRRRPEGMWLPETAVDLE
                     TLEILAEQGIAFTILSPKQANRTRPINDSRWTDESTGTIDCSRPYLCCLPSGGSIVIF
                     FYEETIAREVAFSRLLENGEGFANRMMDYFFQNQKESGLLSIASDGETYGHHHRFGDM
                     ALAYALHYLESRNLARITIYGEYLSTCPPTHEVEIIENTSWSCPHGVERWRSDCGCCT
                     KGSSFRVTPPGPCPVPATPPGDKRCEIVSRQMWRAPLRKAMDWLCQNLAALYGERMNS
                     YVANPWQARDDYINIILDRSVGTIEAFFSNHAVRTLSKEDKVQVLKLLEMQRNGMLMY
                     TSCGWFFEDIAGIESVQVMRYACRAMQLAREVAGVDWDPEFIRILEKAPGTVPEQGNG
                     AQVYKNFVRTAVVDLSRVGFHYAVSSLVAGSPDTIRIRNYTLRNEALEKAESGNLRLS
                     LGKVFLHSDTTWEENTLMFAVLHRGNLSFVGGATEYVDEMTYGAMRDELRDGFSKIDI
                     PRIMLGLEEHFGSHSYSLRHLFRDGQRKVIYAVLDSTLADKESAFRGMYKQFFPLLSA
                     MKEMQIPPPKVLEDPVWYILNLDLKKILSAKDPDIQHLAVLVGEMTKGKFEPDTATLN
                     FTASAAITALMQRLLKSPDDTFLIEKINEVFTILCPLALKYNLWESQNYYFRIGRKKA
                     AGMQVLAGSENAHAREWIRQFEQLGCYLGVKFL"
     gene            complement(5651..5800)
                     /locus_tag="VFC43_00575"
     CDS             complement(5651..5800)
                     /locus_tag="VFC43_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29719.1"
                     /translation="MRSWILCGNRKIDHPAICAERFDTALLYHTCRFFDYVDLCKKKG
                     REGSE"
     gene            complement(6134..7240)
                     /locus_tag="VFC43_00580"
     CDS             complement(6134..7240)
                     /locus_tag="VFC43_00580"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HZK29720.1"
                     /translation="MNTRSWLLITVFIIGIAACVVPAAADNVSYIQVSSNPLGALACF
                     DHWNCQNTPTTFATDPNSYHSISVYKDGYQMSTQTIYTNNTGVTLRVMLNLISNPPLT
                     GSLNLDSSPSGADVWVDQRYYGTTPQVIGGLSDGSHSLTLRNAGYYDVTEQFTITAGQ
                     TTTRSPVMSPYTPSSGFGDLRIQSKPVGAAVYVDNNYAGTTISSTALYVTQLKPGSYS
                     VRVTLAGYQIYSQTAAITAGKVTTVQANMVPVSPGPTPYINGQVTVGSSPSGANIYLD
                     NAYRGLTPLTLVDIPQGSHAIILKLNGYQDWQSSVNVVAGSSTDVSGTLASSPQPTPT
                     TLPPQATQAPISLVTIFSGIGICSAAAILHRKSR"
     gene            7496..7831
                     /locus_tag="VFC43_00585"
     CDS             7496..7831
                     /locus_tag="VFC43_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 C-terminal domain-containing protein"
                     /protein_id="HZK29721.1"
                     /translation="MTEDSINRMVNKGVLGADDMKLQTAGKLEEAARKLRSADMSTHG
                     EDIKKIMNDIESQVYQFKKEAGVKYDEMEAGFHESMEPIETMITDHPIPAILVAIGVG
                     VLAGMLIRR"
     gene            7948..8091
                     /locus_tag="VFC43_00590"
     CDS             7948..8091
                     /locus_tag="VFC43_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29722.1"
                     /translation="MAIDVNWGMMGLFSVIAFLVGWFIFGLLGAVVLAIIVMVVMGII
                     QFR"
     gene            8131..8292
                     /locus_tag="VFC43_00595"
     CDS             8131..8292
                     /locus_tag="VFC43_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HZK29723.1"
                     /translation="MDLIWLAVVFLVLALVFYIFGARGIAGFSMEIAKILVIIFVIVA
                     IITFLFGWL"
BASE COUNT         2079 a         2024 c         2188 g         2135 t
ORIGIN      
        1 agaaccagac acgggtccac cccatctcac tcttcagaca acgggactac gtacctgccg
       61 gggatcttgc ccagctgatt ctctgctggc ggattattca ccgccgcatt ttcatggaga
      121 cctcccggta atggcactcc atgggattcc tgcagaggaa aaggttgtac gacgaggagg
      181 atgcaatgat gcagaaaagg aataagcgac cacgtatgaa gggaactccc ccggctccca
      241 aaaaattctc atccgacccg gtgttttctg tgtgctggga gggtccgctc cccgaacctt
      301 cggtcaggac tgcagaagat ctgcggggag ttcttgcaga tccgggatgt accagcaccg
      361 gaccggtgta ttacatgtat cgtgatgtag cccgttcggt aaaagaccgc agctggttaa
      421 ccaggcagca gctccggttt gatatcacgg ttatcccacc gcgtgagatc tgcggtgagt
      481 tcatcaagac aaaggggcac tatcacccgg ataatccatc aggtaccggc taccccgaga
      541 tatatgaagt ccttgccgga gaggcacact acctgatcca ggacatgtat tgttccgatg
      601 tggtgatgat agcggcgtgt gccggggaga tcgttgttgt tccaccgggt tatgggcatg
      661 taactatcaa ccctgccaga actacggttc tccagatggc aaacattgtc tcttcgcagt
      721 tttcaagcaa ctaccagaga tatgaagcgc aacggggggc agcatatttt gagatggtaa
      781 aggaacgttt tgtacagaac ccggcatacg caaaacctcc gcctctccga tgtgtgaagg
      841 cacagcggct cgcgggcgta aggaatacga taactgcccc gctctacgat ttgattgaaa
      901 agcgggctcc ggtcctggaa tttctcaatt atcccgaaaa ctatgcatca ttattccggg
      961 aactctgccc gtaaacactt atttttagta attcaagttc tgtagaaacc ggcatgaatg
     1021 ggagttcaca ccagaacaat gaaaatctta tttttacgat gaactcccgc ctctgggcct
     1081 cttgagaggc acggcggact gggagttttt tttaaaaata gtcactcatc cgccgcggga
     1141 gcgtcccttc gggggtggcg gcattcatca taactgatcg atagaagggt accaactcct
     1201 aaatcgcatc tgataaacat gattttcaca ggaagtcctc ataaacttct catgacgctc
     1261 ttttgggctt ttgcaattgt tgcctccgcc tccgactctg gtgcagttga agagggatag
     1321 ttcccggaaa ggtaatacag cattatcgta gagcacaggg gcggtccgag tgggacaggc
     1381 acggccccct ccgtcactat ttgttttgtc tttttttaaa aaaacaaggt tttctatcgg
     1441 gagcagtaat tcacgttctc ccatagactt cggtcatatc accgaggttt tcagttaaaa
     1501 gagggctgaa ctggtctttt tccagctgcc atctccagtt gcccgcatcg gtcccgggcc
     1561 ggttcatcct ggattgtgcg ccaatgccga ggatatcctg catggggaac agtaccgtat
     1621 ctgcaaccga catcatggca agccgaatta aagcagcggg tacctgctct gctggaagtt
     1681 cctgcccgat ataccggaag agctggtgtt tctctcccgt tgtagcctcc gattccagcc
     1741 acccgcggac aggggcattg tcatgggtac cggtatacag gatacagttc tttttcaggt
     1801 tatgcggggc gtgcgggctg ccggaaggat caccggtaaa agcaaagagg aggactctca
     1861 tgcccggcag atcgaaccgg tccatgaggt ccgagacgtc cggggtaata atgccgagat
     1921 cttctgcgat aagcggaaga tcgacaaccc ggtttttcag gacggttaat aatttttcac
     1981 cgggtgcagg aacccacttc ccggttattg cattgtctgc acctgccgga acttcccagt
     2041 acgcgacaag gccacggaag tgatcaattc tcgtataatc gaccagggaa agggtatgcc
     2101 cgatccggtc tatccaccag gaaaagccgg atcgttcatg ctcttcccag cagtagagcg
     2161 ggttgttcca gagctgcccg gttttgctga aataatcggg gggcactccg gcaataacga
     2221 ggggaagcag gttgtcatcg agccggaaca attccgggtg tgtccagaca tcagcactgt
     2281 catagctgac atacatcggt atatccccaa tcaaccggat accttttttt atgcagtaat
     2341 ccctcaaagc ctgccactgc cgaaagaaca tatactggag aaatttttcc cgttcaatat
     2401 ttttttgaag ggtctgtctc atcgcaacca gtgctgccgg atcgcggtat tttatcgcct
     2461 ccggccaggt gctccaggaa cttcccgtat agtgccggtg taatgaggtg aaaagcgcaa
     2521 aatcctcaag ccaccgggaa ttattcctgc agaactcatc atacccggta cggccggtct
     2581 ggtcctgcct gaaccgctca tatgccaggg aaaacaattg ttccttgaac cggatcgccg
     2641 cgtcgaaatc tacgatacca cgaggaaatg ccgggacccc ggaaatatca tgttcgtgta
     2701 aaaaaccgtc ttttatcata cagtccgggc tgatcaggtt tgtattacat gcaaaggtgg
     2761 atatgctgtg atagggggaa ttgtcatatt taggactggt gggatgtatg ggaaggatct
     2821 gccagtaacg ctgttttgcg tccgacagga aatccacaaa ctggcaggca gatggcccga
     2881 gatccccgat gccatatcct gaaaaaagag aggtgatatg caaaaggatg ccgcttcctc
     2941 tttgtttcat acaagagccc ccgcattttt cctggacgta atgatggtgc cgctccctga
     3001 cccggtcact ccatacccta tcataaaaat ttcaccccaa gataacatcc gagctgctca
     3061 aactgccgga tccattcccg ggcatgtgca ttttcggatc ctgcaagcac ctgcataccc
     3121 gccgcttttt tgcgaccgat acggaaataa taattctggg attcccagag attgtatttc
     3181 agggcaagcg ggcataaaat ggtaaagacc tcattgatct tttctatcag aaacgtatcg
     3241 tccgggcttt taagaagtcg ttgcataagg gctgtgatgg ctgcacttgc cgtgaaattg
     3301 agcgtcgcag tatccggttc gaattttcct tttgtcattt caccaaccag gaccgcaagg
     3361 tgttgtatat cgggatcttt ggctgaaaga atctttttga gatccagatt gaggatatac
     3421 cagaccgggt cttcaagtac ttttggcgga ggtatctgca tctctttcat cgcagagaga
     3481 agcgggaaaa attgtttgta cataccccgg aatgcagatt ccttatctgc gagcgtggaa
     3541 tcaagaacag catatattac ctttcgctgt ccgtccctga acaggtgccg gagtgaatag
     3601 gaatgactgc caaagtgttc ttcaagaccg agcattatcc gggggatgtc aattttggaa
     3661 aaaccgtccc ttaattcatc ccgcatcgca ccataggtca tttcgtcgac gtattcggtt
     3721 gcccctccca cgaaactgag gtttccccgg tgaagaacgg caaacataag ggtattttct
     3781 tcccatgtag tatctgaatg caggaagact tttccaagtg acagtctgag attgccggat
     3841 tctgccttct ctaacgcttc gttccgcagg gtataattcc gtatccggat cgtatcagga
     3901 gatccggcaa ccagtgagga cactgcatag tgaaatccaa ccctcgaaag atcaacaact
     3961 gctgtccgga caaaattttt gtacacctgt gcgccattcc cctgttccgg tacggtgccg
     4021 ggagcttttt ccaggatacg gataaattcg gggtcccaat ccacccccgc tacttcccgt
     4081 gcaagctgca ttgcacgaca ggcatacctc attacctgca cggattcgat accggcaata
     4141 tcttcaaaaa accacccgca gctcgtatac atgagcatac catttctctg catctcgagc
     4201 agcttgagta cctgtacctt gtcttccttt gaaagtgtcc gtaccgcatg gtttgagaag
     4261 aaggcttcga tggttcccac ggaccggtcc agaatgatgt tgatataatc atcccttgcc
     4321 tgccagggat tggcaacata cgaattcatt ctctcaccgt agagtgccgc aaggttctgg
     4381 cagagccagt ccattgcctt acggaggggt gccctccaca tctgccgtga gacgatctca
     4441 catcttttgt caccgggagg tgtagccggg accgggcagg gaccaggagg agtcacccgg
     4501 aaactggaac ctttggtaca gcatccgcag tcacttctcc agcgctcgac accgtggggg
     4561 cagctccatg acgtattttc gataatttct acctcgtggg tgggtggaca ggtgctcagg
     4621 tattcgccat agatggtaat ccgggcaaga tttctggatt caaggtaatg aagggcgtac
     4681 gccagcgcca tgtcaccaaa gcggtggtga tgcccgtaag tttccccgtc agatgcaatg
     4741 gataacagcc cggattcttt ctggttctga aaaaaataat ccatcatccg gttggcaaac
     4801 ccttcgccat tttccaaaag acgggaaaac gccacctcgc gggcaatggt ttcttcataa
     4861 aaaaagatga caatcgaccc gccggagggc agacagcaca ggtacggccg ggaacaatca
     4921 atggtccccg tactttcatc cgtccaccgg gagtcattga tcggtctggt acggttggcc
     4981 tgttttgggg agagtatcgt aaacgcaatg ccctgttctg ccagaatttc cagggtttcg
     5041 aggtcaactg ccgtttccgg cagccacatc ccttcaggcc gtcgtctgaa ccggaagata
     5101 aaatcctgta ttccccacac tacctgtgtg cgcttgtccc tgtggctggc aaggggcata
     5161 ataatatggt tgtacacctg tgcaattgct gacccgtgcc cggaaaaccg attaatgctg
     5221 tccttatctg cttttagaat ggctgagtat acgtccggct ggttgtgctc cagccaggta
     5281 agcagggtgg gaccgaaatt aaaactgatc cgggaatagt tattgacaat atcaatgatc
     5341 cgtttatctg aatcgagaat ccgggatgcc gcattcggtg catagcattc agcagcgata
     5401 cgttcattcc agtcatgata cggataggca gaatcctcga tctcgacttc ttcaagccag
     5461 ggattttcgc gtgggggctg atagaagtgg ccatggatac agacaaaacg actcatgact
     5521 ccctcatatc acaaaaaccc gcagatatgg ctggatcatt tccgggtatt ccccggtctc
     5581 ttttcccaga taataatctt ctgtgcggtt aggtatcagg agtattagta tcagcagtat
     5641 ccctccctct tcattcactc ccctcccgcc ctttcttctt gcacaggtcc acataatcga
     5701 agaacctgca ggtatggtac aaaagagcgg tatcgaaccg ttcggcacag atcgcagggt
     5761 gatcaatttt tcggttaccg cacagtatcc atgaacgcat ggcagtccgg gcaccaccat
     5821 ctgacgggaa tgtagtgctg ttttgtgttt ttcgtcatag acacggatat aggtccttga
     5881 aatatactgc tgctgtgtcc ctgtatgtag gacagtccgg cagtcattcg ggcagggatg
     5941 gcttgtgcac atacaatgat atccctgtgg atgtggcacg ataaacgttg gacgggtcgt
     6001 ttcttccacg gcaggctttt ttgggtgggt tttttttagg aaatgggggc tgatcaggcc
     6061 cggtttgggg gcgggaggtg ataacgggac gtgcattgaa ggaaatctct tctttgggag
     6121 aaaaaatggt gtattatctg cttttcctgt gcagaatggc agcggcgctg catatcccaa
     6181 tgccggagaa tatggttacc agtgagattg gggcctgggt tgcctgaggg ggtaatgttg
     6241 tcggggttgg ctgcgggctg gatgcgagcg ttcctgacac atccgtgctg ctgccggcga
     6301 ccacgtttac cgatgactgc cagtcctggt acccgttcag tttcagtatg atggcatggc
     6361 ttccctgggg aatatccaca agagtaagag gtgtcaggcc cctgtatgca ttgtcaaggt
     6421 aaatgttggc accggacggg cttgatccga ccgtgacctg gccatttatg taaggggtcg
     6481 ggccgggcga aacaggtacc atgtttgcct ggaccgtggt aactttcccg gccgtgatag
     6541 cggcggtctg ggaatagatc tggtaacctg ccagggtaac gcggaccgaa taggatcccg
     6601 gtttgagctg ggtgacgtag agtgcggtag atgaaattgt agtacctgca tagttattgt
     6661 caacgtagac cgctgctccc accggtttgg attgtatccg gagatcacca aatccggatg
     6721 agggcgtata cggagacatc accggcgacc gggtggtggt ctggccggct gttatggtaa
     6781 attgttccgt aacgtcatag taccctgcat tcctgagcgt cagggaatgg ctgccatcag
     6841 aaagaccacc aatgacctgc ggggttgtac cataataccg ctggtcaacc cagacgtcag
     6901 caccgctggg actggaatcg aggttcaggc tgccggtcag cggcgggttt gagataaggt
     6961 tgagcattac acgtagtgtt acaccggtgt tgtttgtata gatggtctgg gtagacatct
     7021 ggtacccgtc tttgtagacc gagatggaat ggtaactgtt cgggtctgta gcgaacgtag
     7081 ttggtgtatt ctgacaattc cagtggtcaa agcatgccaa tgcacccaaa gggttcgaac
     7141 tgacctgaat atacgaaacg ttatctgctg ctgcgggtac tacgcaggca gcgatgccaa
     7201 taatgaagac cgtgataagc aaccacgatc gtgtgttcat gaagttctgg aacatgtcca
     7261 gacatccttt ttatcgttat aataacatat ccccatgttc taatttttgc aattccccga
     7321 tgtccatccg ttctatggca tgattcaaat tttgtaaaca acagacaggt ctttaaataa
     7381 ttccggagat gtactcgctt gtcctgatat caaccaacag agtacggtat tttccttatg
     7441 tttcccggaa gcatcaggaa aaaatcctat cagaaaggac gattggagca aaattatgac
     7501 ggaagactca attaacagga tggtaaacaa gggcgttttg ggggctgatg acatgaagct
     7561 ccagaccgca gggaaactgg aggaggctgc ccgtaagctg cggagtgcag acatgtcgac
     7621 gcacggggaa gatatcaaga aaatcatgaa tgatatcgaa tcccaggtgt accagttcaa
     7681 aaaagaggct ggtgtaaaat atgatgaaat ggaagccggt ttccatgaat ccatggaacc
     7741 gatagagacg atgattaccg atcaccccat ccccgcaatc ctggtagcga ttggcgttgg
     7801 ggtcctcgcc ggtatgttga tcagaagata atccagcgat tgatgcgcag tgaatctgcc
     7861 gccaccaaag atctggattg ccctgcacac acggccaggt gaagtgtaca tgatgaaaaa
     7921 aaataaggaa gaaggagatt aaaataaatg gcgatagatg tgaattgggg aatgatggga
     7981 ttattctccg tgattgcatt cctggtaggc tggttcatat ttggtttact gggtgccgtg
     8041 gtattagcta tcattgtaat ggtggttatg ggtattatcc agttcagata ataccccgtt
     8101 aaacaaccgt attaaggagg taagaagttc atggatttga tttggcttgc tgtagtattt
     8161 ctcgtactgg cactggtgtt ctacatattt ggtgcacggg ggatcgccgg tttctcgatg
     8221 gaaatcgcga aaattctcgt aataatcttt gtcatagttg cgattattac attcctgttt
     8281 ggctggttat aagaacactg cgtttgagaa gaaaaaatga catcacacaa acattcagac
     8341 aagcaatcta ttgatatttt tattgaagat tttgaattcc tgaaagaggg aatgcaaccg
     8401 gaatgtgatt gttcaagcca tgctct
//
LOCUS       DASRLV010000018         4506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_816713,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000018 DASRLV010000000
VERSION     DASRLV010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816713"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1614
                     /locus_tag="VFC43_00600"
     CDS             <1..1614
                     /locus_tag="VFC43_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HZK29724.1"
                     /translation="DGINSAGALVAQLLNVLGYSVYLYFDYPSLIRGGHNFAVIRGSE
                     QDIGACRNTVDFILALNNETIERHKDSTRPDTAIIFNADLVKSSGQGVPVKAILTDEH
                     APPIMGNSAIIGGFAKAAGIEWDIVVAVFNSSIPKGVDLNLKVAKRAYDQLQIVRPVS
                     KGPNPPRPLLTGNEAIGLGMVRGGLDAYVSYPMTPSSSLLHFLAENQEKFGIRVVHPE
                     NEIGVILMALGFVYAGKRAAVGTSGGGFCLMTEGLSLAGMAELPLVLLVSQRTGPSTG
                     LPTYTAQSDLHFICHAGQGEYPRLVVAPGDAQEALAWSEIAMNVAWKFQVPAFILSDK
                     TLSEGTYSTSPAEIPENLFKEPPAWTGVLPYLRYADLPSGVSPLAWPGMKDAVVKVNS
                     YLHDEAGITTEEAALVNQMTKKRLRKGEGLAEEMLKYPGVAISGNPEATTAVLCWGST
                     KGVCNEIAQELGLRVIRPIVLLPFPAGVVKKALTGIKKVIAVEENATGQLARLAEQDG
                     ITVQHRILQYDGRPFGPEDLLKKVKEVIV"
     gene            1611..2462
                     /locus_tag="VFC43_00605"
     CDS             1611..2462
                     /locus_tag="VFC43_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZK29725.1"
                     /translation="MTGRQLITDVQNTWCPGCGNFSIQHTLKNILADMEQQGKSLDDV
                     VLVTGIGCHAKMADYLNINTFYTLHGRTIPVATGIKLGNPRLTVICCTGDGDSYAEGL
                     DHLIFAAKRNIDITVIVHNNRVYGLTTGQYTPTSPLGFKGKSTPAGTAEYPINPLELM
                     LASGATYIARGYTKKMDLLKTLVVDGIAHKGFSFIDVLQICATFFPAADYYNPRVYEL
                     SGHDPADFESACMKAKEWDYNSDARIALGTFFKRSLPTFDDKMGIQGEKVKDKKQAVR
                     DFLGMKP"
     gene            2598..3401
                     /locus_tag="VFC43_00610"
     CDS             2598..3401
                     /locus_tag="VFC43_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29726.1"
                     /translation="MNDQPSAALQAATLRLLVIPFAVYAAWVLEIFLLAGTASVFMRF
                     NPTGIFVYSIIACIITGLLAPLVCLRAAFLSGAVNMFQIGFRSLRRTLLSSFLTCVGI
                     YGAIMFFNPFGTDRLAFANAFILLLPTAIASVMMCWVLMGTHVQAFVRGGGALISLSV
                     GISATTVLYCVATLSSVSSIVDQSFFLSAVALGIIAAFFFFAVRDIYAASLFVGTGSV
                     FCMADRIDPLYLQNTGQVVWISSVLAVCTLIVIHWYLSRNYVTVKIPVN"
     gene            3543..4148
                     /locus_tag="VFC43_00615"
     CDS             3543..4148
                     /locus_tag="VFC43_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HZK29727.1"
                     /translation="MADLVLTPEIITYIVLPFMIFCARICDVSLGTIRVIFISKGIKY
                     LAPLIGFFEVIIWLLAIGQVMNNLTNVVAYIAYGAGFAYGTFIGMLIEEKISLGLTSV
                     RIITKEDPLELTKYLRSQDYGVTSIDGEGGSGKVKMVFTIIKRQDLHHVVGIIKQFHP
                     NAFYSIEEVKSIAEGVFPETHARSFGIFSWIDSLRFYRKGK"
     gene            4252..>4506
                     /locus_tag="VFC43_00620"
     CDS             4252..>4506
                     /locus_tag="VFC43_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HZK29728.1"
                     /translation="MTGKKILTFIIAIGICLLAGYIGSYYTTPSITTWYAGLHKPDLN
                     PPSWVFAPVWTFLYILMGISLYLTIQSGLKNSEVQFGLILF"
BASE COUNT         1035 a         1169 c         1150 g         1152 t
ORIGIN      
        1 gacgggatca acagtgcggg tgctcttgtt gcccagctgc tcaatgttct tggttattct
       61 gtctacctgt acttcgatta tccctcgctg atccgcgggg ggcataactt tgcggtcatc
      121 cgaggttcag aacaggacat cggagcctgc cggaataccg tggatttcat tcttgcgctc
      181 aataatgaga caatcgaacg ccataaggac agtacccgcc cggatacagc gatcattttc
      241 aatgcggatc tggtgaaatc ctccggccag ggtgttccgg ttaaggcaat ccttacagat
      301 gaacatgccc cccctatcat gggaaactcg gcaattatcg gagggtttgc aaaggcagcg
      361 ggtatcgaat gggatattgt tgttgcagtt ttcaattcct ctattcccaa aggagtcgat
      421 ttgaacctga aggtcgcaaa gagggcatat gatcagcttc agattgtgcg gcctgtgagt
      481 aaaggaccaa accccccccg tccccttctc accgggaatg aagctattgg cctggggatg
      541 gtcaggggag ggcttgatgc ctatgtctcg taccccatga ctccctcctc aagcctgctc
      601 catttccttg ccgaaaacca ggagaagttc ggcatcaggg tcgtgcatcc ggaaaatgag
      661 attggtgtta tcctcatggc actcggcttt gtctatgccg gtaaacgggc agcagtcggc
      721 acctcgggag gcgggttctg cctgatgacc gaaggactct ctcttgccgg gatggcagag
      781 ctgcccctcg tgcttctggt ttcccagcgg accgggcctt cgaccggcct tcccacctac
      841 accgcccagt ccgatctcca ctttatctgc cacgccggcc agggagagta cccccgtctg
      901 gttgttgccc cgggtgacgc acaggaagcg ctcgcctggt cagaaatcgc gatgaatgtg
      961 gcatggaaat tccaggtccc ggccttcatc ctctcagata aaacactgtc tgagggcacc
     1021 tacagtacca gcccggcgga aataccggaa aatcttttca aagaaccacc tgcctggacc
     1081 ggggtactcc cgtatctccg gtatgcagat ctaccttccg gtgtttcacc ccttgcctgg
     1141 ccgggaatga aagatgcggt ggtcaaggtg aacagttacc tgcatgacga agctggtatt
     1201 acaaccgaag aggctgctct tgttaaccag atgaccaaaa aacggttgcg caagggggag
     1261 ggcctggctg aagagatgct gaaatacccc ggtgtcgcga tttctggcaa tcctgaggca
     1321 acaacggcgg tgctgtgctg gggatccact aagggtgtgt gtaatgaaat tgctcaggag
     1381 ctcgggctgc gggtgatccg tccgattgtc ctcctgccct tccctgctgg agtggtaaaa
     1441 aaggcattaa ccgggataaa aaaagtgatt gcagtcgaag agaatgcaac ggggcaactc
     1501 gcacgacttg cagaacagga cgggattacc gtacagcacc ggatccttca gtatgacggg
     1561 cgcccgttcg gtccggaaga tctgctcaaa aaagtaaaag aggtgatcgt atgaccggac
     1621 ggcagctcat aaccgatgtc cagaacacct ggtgccctgg gtgcgggaat ttctctatcc
     1681 agcataccct gaaaaatatc cttgctgaca tggagcagca gggcaaatcg ctggatgatg
     1741 tcgtgctcgt gacgggtatc gggtgccacg cgaaaatggc cgattatctc aatatcaaca
     1801 ccttctatac gctgcacggc aggacaatcc ccgttgcgac gggtatcaag ctgggcaatc
     1861 cccggctgac agtcatctgc tgtaccggtg acggggattc gtatgctgaa gggctcgacc
     1921 acctgatctt tgccgccaaa cgcaatatcg acatcacggt catcgtccac aacaaccggg
     1981 tttacgggct tactaccggg caatacactc ctacatcacc gctggggttc aaagggaaat
     2041 ccacaccggc aggcaccgct gaatacccga tcaatcccct tgagctgatg cttgccagcg
     2101 gagccacgta cattgcccgg ggatatacca aaaagatgga cctgttgaag acgctggtcg
     2161 tagatggcat agctcacaag ggtttttcgt ttatcgatgt gctccagatc tgtgccacct
     2221 tctttccggc tgctgactac tataacccgc gggtatacga actatccggt cacgacccgg
     2281 cagattttga atccgcgtgc atgaaggcaa aagaatggga ttataacagc gatgcacgga
     2341 ttgcactcgg tacattcttt aaacggtccc ttccgacgtt tgatgacaaa atggggatac
     2401 agggggagaa ggtaaaagac aaaaaacagg ccgtccggga ttttcttggc atgaagccat
     2461 gatctcctct ccctcaataa tccgcacaat cgctgaatga atccagacaa tcgggaatat
     2521 actttgacct gcaccacacg ggtacctgca atggcacaca aatacactac gcgaaaaact
     2581 cccggacggt ggaacgattg aacgaccaac cttcagccgc attacaggct gcgaccctgc
     2641 ggctcctggt aattccgttt gcagtctatg ctgcatgggt gcttgagatc tttttactcg
     2701 ccggaaccgc atccgttttc atgcgtttta atccaaccgg aatcttcgtg tacagtatca
     2761 tcgcctgcat catcaccggt ctccttgcac cgctcgtgtg cctgcgggca gcgtttttat
     2821 ccggtgcggt caacatgttc cagataggat tccggtcatt gcggagaact ttgcttagca
     2881 gtttcctgac atgtgttggt atctacggag cgatcatgtt tttcaatccc ttcgggaccg
     2941 accgccttgc gtttgccaac gcctttattc tcctgctgcc caccgcgatc gcatcagtga
     3001 tgatgtgctg ggtgcttatg ggaacccatg tgcaggcatt tgtccgcggc gggggggctt
     3061 tgatttccct gtcggtgggt atttcggcaa caaccgtgct ctactgcgtt gcaacgttat
     3121 cctctgtatc ttccattgta gaccagtcct tctttttatc ggcggtcgct ttgggaatta
     3181 ttgctgcatt tttcttcttt gccgttcgcg atatctatgc agcatctctt tttgtcggga
     3241 cagggagtgt cttttgcatg gctgaccgga ttgatccact gtatctgcaa aacactggtc
     3301 aggttgtctg gatcagttcg gtgcttgcgg tgtgtacgct tattgttatc cactggtatc
     3361 tttcacggaa ttatgtgacg gttaagattc cggtaaacta aatccgaatc tcgtgttccc
     3421 tgatagtcat ctgattccgg ctgttttgaa gttgtgagat ggttttttcc ggtcttatat
     3481 gatcaaggaa tcgcaccata ccacaaacta ttaacacccg cgatcgaaaa aatataacaa
     3541 gaatggccga tctggtcctg actccggaaa ttattaccta tatagtcctg ccattcatga
     3601 tcttctgtgc ccggatctgc gatgtgagcc ttggcactat ccgggtgatc ttcatatcca
     3661 aggggatcaa gtatctcgct cccctcatcg ggtttttcga agtgatcatc tggctgcttg
     3721 caatcgggca ggtgatgaac aacctgacaa atgtcgttgc gtatatcgcg tatggagccg
     3781 ggtttgctta cgggacgttc attggcatgc ttatcgagga aaaaatatca ctcgggctca
     3841 ccagtgtccg catcattacc aaagaagacc ccctggaact cacaaaatac ctccgttcgc
     3901 aggattacgg ggtaaccagt atcgatgggg aaggtgggtc cgggaaggta aaaatggtct
     3961 ttacgatcat caagcgacag gacctgcacc atgtcgtagg catcatcaag cagttccacc
     4021 cgaatgcctt ctattccatt gaggaagtaa aatccatcgc cgagggagta tttcccgaaa
     4081 cacatgcccg cagttttggt attttttcct ggatcgattc tttgcgtttt taccggaaag
     4141 gaaagtgagg ttcttttggg aaaatgggtt atctggcgcc tgtattgaca gaactttatt
     4201 catccaataa tcttttatgc caagcgtttc acatagtagc tttgagattt aatgacggga
     4261 aaaaagatac ttactttcat tatcgcgatt ggtatatgcc tgctggcggg gtatatcggc
     4321 tcatattata caaccccctc tattaccaca tggtatgccg gcctgcacaa gccggatctt
     4381 aatcccccgt catgggtttt tgccccggta tggaccttcc tctatattct catgggaata
     4441 tccctgtacc tgaccatcca gtccgggctt aagaattcgg aagtgcaatt cggcctgatc
     4501 ctcttt
//
LOCUS       DASRLV010000019         4270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_572962,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000019 DASRLV010000000
VERSION     DASRLV010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572962"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VFC43_00625"
     CDS             complement(<1..942)
                     /locus_tag="VFC43_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HZK29729.1"
                     /translation="MFKPEQMSRLLIVASKDQMAPIVTELHRNNLFHIEDYVEAGAEG
                     YEGFKIGTPLSGATEASGDLVKIRTIQNTFSIRSDELEVKKRQKQSDLQVMIERDLPA
                     IEQEVEGLTVKRSKLEIKVKEYEQKMSELSPFIGIPGDLDLYRGYSTLSVFAGYVEKE
                     VSLSVPHEMFSTKGKGKHFMVVIAAVEQRSVVEAALQEAGYQAVTIPDDSGDPKERIS
                     FYTGQITLMKKEMGEINQNLDAIKEKHANFMVACEEILKVQVERAEAPLRFATTNETF
                     VAEGWVPSASVENLSQALVKATAGKIFVTVLPTDSEHD"
     gene            complement(929..1258)
                     /locus_tag="VFC43_00630"
     CDS             complement(929..1258)
                     /locus_tag="VFC43_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HZK29730.1"
                     /translation="MKTEVLRDIKKTEEDYQKTISAAQEEKKHRHSQVELESDNLVTK
                     AQSNAEQYKKLKLEEARHQAALKHAEIIKSGNQRAAAIREKGSKNLQKAVQLLVVRFK
                     EQLHVQA"
     gene            complement(1374..1847)
                     /locus_tag="VFC43_00635"
     CDS             complement(1374..1847)
                     /locus_tag="VFC43_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZK29731.1"
                     /translation="MNYTLDDELLLIGGKQSFLLVAKVKGRFGLCIETSDDEYIQGVE
                     AGDLVVVSAPEGGAVEPALMLIDLVRTFHVPLLVLPKNHPGSRRISYVVSVGPEIFTS
                     CSIQRGTHPEQHLICSSGELAGIILKGEGGGVQISSLHGDITVRHIKFKTETVFS"
     gene            complement(1844..1999)
                     /locus_tag="VFC43_00640"
     CDS             complement(1844..1999)
                     /locus_tag="VFC43_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29732.1"
                     /translation="MCTVGGPVDIDLPPDRVQGKDYRCNECGERFKGIGKKPICPSCQ
                     SDDVAKA"
     gene            complement(2071..2334)
                     /locus_tag="VFC43_00645"
     CDS             complement(2071..2334)
                     /locus_tag="VFC43_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HZK29733.1"
                     /translation="MSDVLVVILLLIAIIAVVYYLVKKFTVLLVNAILGIIVLFFLNF
                     LHIMQWMGKPDLGYSLATILICAVGGLPGVCILVLLNILGITL"
     gene            complement(2346..3632)
                     /locus_tag="VFC43_00650"
     CDS             complement(2346..3632)
                     /locus_tag="VFC43_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HZK29734.1"
                     /translation="MAWIDQLLQKGQKAVEEVEVFYVEGTSVSADLKKKNVSLATTSV
                     DCGLGIRTIHKGRIGSSSTNNPDKWEECLSAAIASGKLATFQEWGGLPDPARFSDEPL
                     SFDSSIRLEPDLVVGMIGQMLEGADQHPAEVTAGSASLSSATITLANSRGVRYTSRHT
                     GVSLSLEAIHKQSTGYEFDHACFLDKVDPVNVGKKATFFAVESDNGKDIATGEYEILL
                     SPLAYGELLGNVFVPALSGRNVHAGRSRLSQSLGKSVTDPSIAMYDDPLLKKANGSVH
                     WDAEGIPARRTDFIKDGILETFAYDLKTAYRFNKTSTASAIRGGYGGLPSIGHHNFIV
                     DGKRDEVADERVLYIHNVVGAHTANPMSGDFSVELSNAFWMNAGEYEAPIRSAMLTGN
                     VFDMHHEISGLSKESRAIGSMILPSVKIHKQRIIGK"
     gene            complement(3619..>4270)
                     /locus_tag="VFC43_00655"
     CDS             complement(3619..>4270)
                     /locus_tag="VFC43_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HZK29735.1"
                     /translation="AVGHASEGDLIQEGNSVLKGKTGQKIGNDNLTIVDDPGIPEFGF
                     DPVDAEGVAVSRTEIIKNGIINAFLHNRETLSSVGYGTVGHARAMPGEPPLVRMSNTF
                     IEAGDATRDEIFEECRDGIFLKGSRGGQVDPGRGIFQFNAEYGYLIEKGECTKMVRDV
                     SLSGEILKTLHGISLCGNVRTMSPGYCGKGGQSVPVSDGAPYILLCDAVVGGNGMD"
BASE COUNT         1056 a         1131 c          891 g         1192 t
ORIGIN      
        1 atcatgctca ctgtctgtgg gcagaacggt gacaaagatt tttcccgcgg tggctttgac
       61 cagagcctgg ctcaggtttt caacgcttgc cgatggaacc cagccttctg caacaaacgt
      121 ctcgtttgtc gttgcaaacc gcaaaggtgc ctcggcccgt tcaacctgga cttttaaaat
      181 ttcctcgcat gcaaccatga agtttgcatg cttctcttta atcgcatcca agttctggtt
      241 gatctccccc atctcctttt tcatgagggt gatttgcccg gtgtaaaagg aaatacgctc
      301 cttgggatct cctgaatcat cagggatcgt gactgcctgg tatcccgctt cctgtaaagc
      361 tgcttcaacc acacttcgtt gctcaaccgc agcgatcacg accatgaaat gttttccttt
      421 acctttcgtg gagaacattt catgaggtac agaaagagag acctcttttt caacataccc
      481 ggcaaaaacg gaaagtgtcg aatatccgcg atagagatcg agatctccgg gaatacctat
      541 aaaaggagag agctcgctca tcttttgttc gtactctttt acttttattt cgagttttga
      601 gcgttttacg gtaagtcctt caacctcttg ctcaattgca gggagatcgc gttcaatcat
      661 gacctgaagg tccgactgct tttgtcgctt ctttacctcc aactcgtcgc tgcggattga
      721 gaaggtattc tggatagtac gtatctttac aagatcgccc gatgcttctg ttgctcctga
      781 aaggggcgta ccaatcttaa aaccctcata accttcagct cccgcttcaa cgtaatcctc
      841 aatatgaaac aggttattgc gatggagctc cgtaacaatg ggagccatct ggtcctttga
      901 tgcgacaatc agcagcctgc tcatctgctc aggcttgaac atgcagctgc tccttaaacc
      961 gcacaaccag caactgcact gctttttgaa ggttcttcga acctttttca cggattgccg
     1021 ctgcacgctg attgccactt tttatgattt cggcgtgctt gagtgccgcc tggtgccgtg
     1081 cttcttccag tttaagcttt ttatattgtt cagcattgct ctgcgctttt gtaaccagat
     1141 tatcagattc cagctcaacc tgagaatgcc tgtgcttctt ctcttcctgg gctgcactga
     1201 ttgttttttg gtaatcctct tcagtttttt taatgtctcg taggacctca gtcttcatcc
     1261 aaccctcctc tcacggcagt acaataattg gtgaaaaaaa caatatatgt attgttattt
     1321 acttttcaaa aagtcagaac atcaacgcga aaatgataaa attctccgat aattcaggaa
     1381 aatacggttt ctgttttgaa ttttatatgc ctgactgtaa tatcgccatg caatgaggaa
     1441 atctgaactc ccccaccttc tcctttcagg attatccccg cgagttcacc gctcgaacaa
     1501 atgagatgct gttccggatg cgttcccctc tgaattgaac agctggtaaa aatttctggt
     1561 cctacggata ctacatagga gatccgtctt gatccgggat ggttttttgg caataccaat
     1621 aaaggcacat ggaaggtccg gacaaggtcg atgagcatca gcgcaggttc aacagccccg
     1681 ccttcaggag cagaaacaac aacaaggtcc ccggcttcaa caccctggat gtattcatca
     1741 tcggatgtct cgatacaaag accaaacctg cccttcactt ttgcgacaag taaaaaagat
     1801 tgttttcccc cgatgagcaa cagctcatca tcaagggtgt aattcatgcc ttggccacat
     1861 catccgactg gcagctggga cagatgggtt ttttaccgat tcctttgaac cgctcaccgc
     1921 attcgttgca gcggtaatcc ttcccctgaa cgcgatccgg ggggagatca atatctacag
     1981 gtccaccaac agtacacata tccacatcac ctcactacga aatctctgcg acattattta
     2041 atggtatctc atttttttgt ttttgtgttt tcacagggtt attccaagaa tattcagcaa
     2101 aacaagaatg cagacacccg gaagtccccc aacggcacat atcagtattg tcgcaaggct
     2161 gtagccaaga tcgggtttac ccatccattg catgatgtga agaaaattca ggaaaaacaa
     2221 tacgattatt cccaaaatgg cgttcacgag aaggaccgtg aatttcttta ccaggtaata
     2281 aacgactgca ataattgcta tgaggaggag tatgacaacc agcacgtcac tcatgactgt
     2341 tacctctatt taccaataat acgctgttta tgtattttta ccgacggcag gatcatagat
     2401 ccaattgccc gggattcttt gcttaatccg gagatttcat ggtgcatatc gaacacattt
     2461 ccggtaagca tcgcactcct tataggcgct tcatattctc cagcattcat ccagaacgca
     2521 ttagaaagct caacagaaaa atcaccactc attgggtttg cagtatgagc tcccacaaca
     2581 ttgtgaatat agagcacccg ctcatcagct acttcgtccc gttttccgtc aacaataaaa
     2641 ttatggtgtc cgatcgaggg taaacctcca taaccaccac gtattgcact cgcggtgctt
     2701 gttttgttga aacgatacgc tgttttgaga tcataggcaa aggtttccag aatcccgtct
     2761 ttgataaaat ctgtccgtcg tgcaggtatt ccctctgcat cccaatgcac acttccattc
     2821 gcctttttca gcagtggatc atcgtacatc gctattgacg gatcggtaac actttttccc
     2881 agggattgcg acagacggga ccttccggca tgtacgtttc ttccactcag ggcaggaaca
     2941 aacacgttcc caagcagttc cccatatgca aggggggaga ggagtatctc gtattctccc
     3001 gtggcgatat ctttcccatt gtcagattcc acggcaaaga acgttgcctt ctttccgaca
     3061 ttcaccggat ccaccttatc gaggaaacag gcatgatcga actcataccc tgttgactgc
     3121 ttatgaattg cttccagtga gagcgaaact cccgtatgcc tgcttgtata ccggacacca
     3181 cgactgtttg ccagcgttat tgttgcagag gaaagtgagg cggaacccgc agtaacttca
     3241 gcagggtgct gatctgcacc ttcaagcatc tgtccgatca tcccgacaac gagatccggt
     3301 tcaagtctga tagaagaatc gaatgagagt ggctcgtcgg aaaaacgtgc aggatcgggc
     3361 aatcctcccc attcctgaaa cgttgcaagt ttcccgcttg caattgctgc gctaaggcat
     3421 tcctcccact tgtcaggatt gtttgtactc gatgacccta tccttccctt atggatagta
     3481 cggattccaa gaccgcaatc cactgacgtc gtggccagac ttacgttctt ttttttcagg
     3541 tcggcagaga ccgatgtgcc ctcgacataa aaaacttcaa cttcctcgac ggctttttgc
     3601 cctttttgga gcagttgatc aatccatgcc attgccaccc accaccgcat cgcacagaag
     3661 tatatagggt gccccgtcgc taacgggaac actctggcct cctttcccgc aatatcccgg
     3721 gctcatggtg cgaacgttcc cgcagaggga gatcccatga agcgttttca ggatttcgcc
     3781 tgataatgaa acatcccgca ccatttttgt acattcaccc ttttcgatca ggtaaccata
     3841 ttccgcattg aactggaaaa ttcccctacc gggatctacc tgtccaccac gagaaccttt
     3901 gaggaagatc ccgtcgcggc attcttcaaa gatctcatca cgcgtcgcat ctccggcttc
     3961 gataaaagtg ttgctcatcc gtacaagagg gggttctccc ggcattgccc ttgcatggcc
     4021 gacagtgcca tatcccacag atgacaacgt ttcgcggtta tggagaaagg cgttgataat
     4081 accatttttg atgatttccg tccggcttac tgccacccct tctgcgtcca ccggatcgaa
     4141 accaaattcc gggatcccgg gatcatcaac aatcgtcagg ttatcgttcc cgatcttctg
     4201 tcctgttttt cctttcagaa cagaattgcc ttcctggata agatcacctt cgcttgcatg
     4261 gccaactgct
//
LOCUS       DASRLV010000020         6059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_411717,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000020 DASRLV010000000
VERSION     DASRLV010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6059
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411717"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..617
                     /locus_tag="VFC43_00660"
     CDS             201..617
                     /locus_tag="VFC43_00660"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HZK29736.1"
                     /translation="MKQGVILLVITIILIAVCGCTQTSTPVQPTTTIPSPPQTTQQLT
                     VTTIPVSQTTASVSANTILIKNFAFNPANITVKVGSTVRWVNQDSVPHRLMFTDGIYS
                     QVLAGMDTWSRKIDLAGTYDYVCTIHTDMHGTVIVE"
     gene            complement(800..1003)
                     /locus_tag="VFC43_00665"
     CDS             complement(800..1003)
                     /locus_tag="VFC43_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29737.1"
                     /translation="MMRIKKNKPQISPETKTFREVRRLNLNRTFIPLKIGLIAGYIVL
                     GMLSASTSMFGILLGAMLRIAGL"
     gene            complement(1338..1422)
                     /locus_tag="VFC43_00670"
     tRNA            complement(1338..1422)
                     /locus_tag="VFC43_00670"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1386..1388),aa:Ser,seq:cga)
     gene            complement(1607..3205)
                     /gene="thsA"
                     /locus_tag="VFC43_00675"
     CDS             complement(1607..3205)
                     /gene="thsA"
                     /locus_tag="VFC43_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit alpha"
                     /protein_id="HZK29738.1"
                     /translation="MQSGQQIIILKENVERNTGKEAQRSNIAAAKAIAGAVKSTLGPR
                     GMDKMLVDSTGDIVITNDGATILGEIAVQHPGAKMVIEVARTQDDEVGDGTTTAVVIV
                     GALMEQAEGMLEQGIHPTVIAQGYRMGMEKALEIVNSLSLKVEPTDRKTLFKIVDTAI
                     TGKSIESVKTKLDGIIVDAVMAVAEKTNGKIIVDEEDVMIKKQKGQAMDDAELIRGIV
                     IDKKRISEGMPKKVTKAKVAMIATPLEITKTQVKAKIKITSAEQITAFSEQERDALKK
                     LADSIVGSGANVLLCQKGIADAVQFYLAKNGILAIEDVPEKDMKYAARSLHANIVNKP
                     EALTPKDLGAADLVEEDTDANITRISGCKNPKSITILLRGSSDYLLDELERAVVDGTR
                     VVMDAMEDGTYVVGGGAVETELLMKIRDYASTVGGRVQLAIEAYAAAFESIPRTLAEN
                     SGYNPIDKLVELKNAHSKGKKNAGLNVYDGTIVDMLAEGVLEPLRSKRQSIQSASETA
                     VMLIRVDDMMITQSKSAGGAPGAF"
     gene            complement(3287..3895)
                     /locus_tag="VFC43_00680"
     CDS             complement(3287..3895)
                     /locus_tag="VFC43_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase-like protein"
                     /protein_id="HZK29739.1"
                     /translation="MSSLDDLVGRAKILLAEGHSPGQIADELSLSMETVTWLLTQGRG
                     TAAPKDVHIDWTAVSSNATLLEETSLMLLQRFYGAQTEESVSSPAAGVIVGIALSGIP
                     IATIIAVQEAAKLAIYHPAKQSQSEHPMGSISGNFAAIGGERCIIVDDTITSGNTMRE
                     VVKYLKKHHAIPVAIWVIFDKRGIKDIDGVPVYSLFKISRID"
     gene            complement(3916..4371)
                     /locus_tag="VFC43_00685"
     CDS             complement(3916..4371)
                     /locus_tag="VFC43_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HZK29740.1"
                     /translation="MKVILDSSVFFSDYPVTGELYTTPSVRDELIDIRSKGIFEKFCS
                     AGLHIVSPGIQSRAKVISAAEKTRDTSVISETDCDLLAVALELDAVLYTDDFAIQNVA
                     GVLGIQTIPINQRKAKRIHWKYRCSGCGRYFDHDGECLICGSIIKRKLK"
     gene            complement(4409..5272)
                     /locus_tag="VFC43_00690"
     CDS             complement(4409..5272)
                     /locus_tag="VFC43_00690"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HZK29741.1"
                     /translation="MKVICTEKSQILATSLARALKTTVVEVKFSRFPDGEQYLIAGEL
                     DEETIIVGSVVDNDSLVQLILLIDACDSSENQLVLPYMGYARQDKRFRKGEPISARAV
                     AQVLSRGVSEIITVNIHEKEVLNYFGVPARNISLAGDIGSYIKTLNLKNPLILAPDEG
                     ARTFAEQVASSGGWDFDHLEKTRLSGVEVRMAPRQLCASERSVFIVDDIISTGGTIAT
                     ATSMLYQQGAKDVYAACVHGVLTGGAYVHLMAAGVRDVICSNTIERGCSKISAAGQIA
                     LTLQHNDIKKR"
     gene            5472..>6059
                     /locus_tag="VFC43_00695"
     CDS             5472..>6059
                     /locus_tag="VFC43_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK29742.1"
                     /translation="MEISEQSSPQKEAVEIPDSTPDVAHTQNVIATSSQIEVPEKLVD
                     QVIGQEHAVEVIKKAAIQRRHVMMIGSPGTGKSMLAKAMAEILPKEELQDILAYPNSD
                     DPNNPVIRTVTGGRGKQIVTAHKAEAKKKVQFRNTLLMLLLIGIIGYAFITYQWLMGI
                     IAAAFVFMALRYTTPREEAMVPKLLVSNDTKNIAPF"
BASE COUNT         1602 a         1434 c         1298 g         1725 t
ORIGIN      
        1 tcccgcgctc ttccatcaaa tctgctttca gacctgtcct tatctgacca ctatcacgtt
       61 gaagagttca tgaaaaggat aaagagtgaa caatttgtag cgctgaattc cgaccagtga
      121 attcagaaga atacgatgtc cattcaaatt aaaaagctat agggatccgg tattatatag
      181 aaaacagtgg agattaatct atgaaacaag gtgtaattct tcttgtcatt accatcatcc
      241 tgattgcagt ctgtggatgc acccagacat caacacccgt tcagccgacg acaactatcc
      301 cctcaccccc acaaacgaca caacagctga cagtaacaac cattccagtt tcacagacaa
      361 ctgcttctgt atccgctaat acgatcctga taaaaaattt tgcatttaat cctgcaaata
      421 ttacagtgaa agtgggatca acggtacgct gggtgaatca ggattcagtt ccgcacaggc
      481 tcatgtttac ggacggaata tattcacagg tgcttgcagg gatggatact tggagcagga
      541 aaattgatct agcgggtacc tatgattatg tctgcacaat acacacggat atgcatggaa
      601 cggttatagt tgagtgacga atttttttga gagaaggcaa ataacactgt tgaattatca
      661 gtgcatatat gcaggccagt attcggcaca cgagaaatcc cgatattctt tcatcttgta
      721 ttgccggaat cgtgggaaca tccctatttc ctgacataaa atatctgaaa ccaataattt
      781 taaaaaaacc ggtaaaaaat tataacccgg ctatccggag cattgctccg agaagaatgc
      841 cgaacatgga tgtggaagct gacaacatac ccagcacgat atacccggcg attaatccga
      901 ttttcagagg tataaaggta cggttcaggt taagccttct tacttcgcga aatgtttttg
      961 tttctggcga aatctgtggt ttattcttct ttatcctcat catagtggtt caccagtgct
     1021 gatcttggtt cttttcaatg attgtgggca cggccgcttt cttcctgtag ttagcgaata
     1081 ataccttgca tctaatacta tgaataggta ttatttaaaa ttttttttcc gtaataaaaa
     1141 taaaatacct caaaattttg ctttttttaa gattttgtgg caataataaa catattttta
     1201 aaatcgcact gcaaatcgta tatattgttt ttttctgcga agtttaaaat atccatgtgg
     1261 gaatagcgga aaattgtatc cgggaaaaca tgaatcaggg atagaacaat gttggataaa
     1321 gtcagtgaat attaatacgc cgagggggag atttgaactc ccgaggcgcg aagcgccagt
     1381 agctttcgag gctaccgcct tcccgggcta gactacctcg gcaaccggtt ctgatatttt
     1441 ttcaaccaat tgctctaata gttatctgat acctgaacga atcaaatcat tgtatcaggc
     1501 actcggtatt gatggctgtt ttcctggaaa aaattgaatt accaaaaatt caaaaaaata
     1561 aatgaagggg attcatccta ttcctcttct ttttttaact catatcttag aatgctccag
     1621 gagcgccacc cgcagatttt gactgggtga tcatcatgtc atcgacacgt attagcatga
     1681 ctgcggtctc agatgcgctc tggattgact ggcgtttgga tctcaaaggt tcaaggacac
     1741 cttcagcgag catgtccaca attgttccat catacacatt caaccccgca ttcttcttgc
     1801 cctttgaatg cgcatttttg agttctacca gtttgtcaat ggggttatat cccgaattct
     1861 ccgcaagggt ccggggaatc gattcgaatg ctgctgcata tgcttctatg gccagctgca
     1921 cgcgtccacc gacagttgaa gcgtaatccc ggattttcat caggagttcc gtctcaactg
     1981 cgccaccgcc aaccacatac gtgccgtctt ccatggcatc cataactacg cgggttccgt
     2041 caaccacggc gcgttcgagt tcgtcaagga gataatcgct tgaaccacgg aggaggatcg
     2101 taatcgactt gggattctta cacccggata tcctcgtgat attagcatcg gtgtcttctt
     2161 ccacaaggtc tgcagctcca agatcctttg gggtaagtgc ttccggtttg ttcacaatat
     2221 ttgcatgaag tgaacgggcg gcatatttca tatctttttc cggtacatcc tcaattgcca
     2281 ggatcccgtt tttggcgagg tagaactgaa ccgcatctgc aatgcctttc tgacaaagta
     2341 aaacatttgc accgcttccg acaattgagt cggccagttt tttaagggcg tcgcgttcct
     2401 gttcggaaaa ggccgtaatc tgttcagcgc tggtgatctt aatttttgct ttaacctggg
     2461 tttttgtgat ttcgagaggg gttgcaatca ttgcgacttt tgctttcgta acttttttgg
     2521 gcatcccctc actgatgcgc tttttgtcaa tgacaatgcc acggatcaat tcggcatcat
     2581 ccattgcctg gcctttctgc ttctttatca tcacatcttc ttcgtcaaca ataatcttcc
     2641 cgttggtttt ttcagcaact gccatcacgg catcaacaat aataccgtcg agcttggttt
     2701 tcacagactc gatcgacttt ccggtaattg ctgtatcaac aattttaaag agggtctttc
     2761 tgtcagttgg ctctaccttg agcgagaggc tgttcacgat ctcgagggct ttctccatac
     2821 ccatccggta tccctgtgcg atcactgtcg ggtgaatacc ctgttcgagc attccctcgg
     2881 cctgttccat gagcgcacct acaataacga cggcagttgt tgtcccgtca ccaacctcgt
     2941 catcctgggt tcgtgcaact tcgatgacca tttttgcacc cgggtgctgc accgcaattt
     3001 cacccagaat agttgcgcca tcattggtga tcacaatgtc cccggttgaa tctaccagca
     3061 tcttgtccat accccgtgga ccaagtgttg acttaaccgc tccagcgatt gcttttgctg
     3121 ctgcgatatt cgagcgctgg gcttctttcc cggtattacg ctctacattt tcttttaaaa
     3181 tgataatttg ttgtccagac tgcataatgg atcctctgat gttgtaaaag gtggaattga
     3241 acttctatat aaagctactg tgttaggcac taaaaaaaag aattgatcag tcaatgcgcg
     3301 aaattttaaa gagtgaatag accggtacgc catcaatatc cttgataccg cgcttgtcga
     3361 atataaccca tatcgctacg ggtattgcat ggtgcttctt cagatatttg accacttcac
     3421 gcatggtatt tccggacgtt atcgtatcgt caacaatgat acagcgttcc ccgccaattg
     3481 cggcaaaatt tccgctgatt gatcccatcg ggtgctcact ctggctctgt tttgccgggt
     3541 gataaattgc cagttttgct gcttcctgta ctgcgatgat agttgcaatc ggaattcctg
     3601 aaagtgcaat accaacaatc acgccggcag caggagacga aacgctttcc tctgtctgtg
     3661 ccccgtaaaa ccgctgaagg agcatcagtg aggtttcttc gagcagtgtg gcattgctgc
     3721 tgacggcagt ccagtcaata tgtacatctt tcggagccgc agtacccctt ccctgtgtga
     3781 gcagccacgt cacggtttcc atggacaggg agagttcatc tgcaatctga cccggactat
     3841 gtccctcagc gaggagtatt tttgccctgc caaccagatc atcaagagaa gacatgggaa
     3901 aagattcgcc ggtatctatt taagttttct tttaattatc gagccgcaga tgaggcattc
     3961 gccgtcatgg tcgaaatacc ttccacagcc gctgcagcga tatttccagt gaatgcgttt
     4021 tgcctttcgc tggttgatag gaattgtctg tatcccaagc acacctgcga cattctggat
     4081 tgcaaaatca tcagtataca gaacagcatc aagttcaagc gcaacagcga gaaggtcgca
     4141 atccgtttcg gagattacac ttgtatcccg ggttttttcc gccgcactaa tcaccttagc
     4201 tctactctgg ataccaggag aaacaatatg cagacctgct gagcaaaatt tttcgaatat
     4261 cccttttgaa cggatatcga tcagctcatc cctgactgag ggggtcgtgt acaactcccc
     4321 ggtaaccggg tagtcagaga aaaaaacgct ggaatcaagg ataactttca tgtgagaatc
     4381 cggaatagag aaaacaaatc attcctgtct aacgtttttt aatatcatta tgttgtaatg
     4441 tcagggcgat ctggccggca gctgaaatct tactgcaccc gcgttcgatt gtattgctgc
     4501 atattacgtc acgcacaccc gctgccatca gatggacata tgccccgccg gtgaggaccc
     4561 cgtgaacaca ggcagcataa acgtccttag caccctgctg gtacagcatg gatgttgccg
     4621 ttgcgatggt cccgccggta gagataatat catcgacgat aaacactgat cgttctgaag
     4681 cacataattg ccttggtgcc atcctgactt cgactccgct gagacgtgtt ttttcaagat
     4741 gatcaaaatc ccagcctcct gatgatgcaa cctgttcagc aaaggtacgt gccccttcat
     4801 caggagcaag gatcagggga tttttaagat tcagggtttt aatatatgaa ccgatatctc
     4861 ctgcgagcga tatattccgg gcagggactc caaaataatt cagtacttct ttttcatgga
     4921 tattcacggt aataatttct gaaacacccc tgctgagcac ctgagctact gcccgtgcac
     4981 tgatcggttc tcccttcctg aagcgtttat cctggcgggc gtatcccata tatggcaaca
     5041 caagctggtt ctctgaactg tcacaggcat cgattaaaag aattaactgg acaagtgagt
     5101 cattatccac aacacttccg acaataatcg tctcctcatc aagttccccg gcaatcaggt
     5161 attgttcccc atcaggaaac cgggaaaatt ttacttcaac gactgttgtt tttagcgccc
     5221 gtgccagact ggtggcaagt atctgggatt tttcagtgca gataactttc atagtatctt
     5281 cctaaatctt gagtgctgct gttttttttc ttagttttta ttcacaccat taggtattgt
     5341 acttttttaa gcaatgaacc ggaaggtttc tcctgtgata atcccatccg atgtaattag
     5401 ctgaaagtac ttaaactatc atgcagcaat tgatatgaaa actcccaagt gtaaaagagg
     5461 tatatctcgc aatggaaatt tctgaacaat catccccgca gaaagaggca gttgaaatac
     5521 cggattctac cccggatgtt gcacatactc aaaatgtgat tgcaacatcg tcccagatcg
     5581 aggttccgga aaagctggtt gatcaggtta tcggtcagga gcatgcggtt gaagttataa
     5641 aaaaggcagc aatccagcga cggcatgtca tgatgatcgg cagcccggga acaggtaagt
     5701 cgatgctggc caaagctatg gccgagattt taccaaaaga agaacttcag gatattcttg
     5761 cataccccaa ttccgatgat cctaacaacc cggtaatcag gaccgtgacc gggggacggg
     5821 gcaagcagat cgttacagca cataaagcag aagccaagaa aaaagtccag ttccggaata
     5881 cccttcttat gctcctgctc attggtatca tagggtacgc attcattacc taccagtggc
     5941 tgatgggcat cattgctgca gcatttgtct tcatggcatt acggtacaca acaccccgtg
     6001 aggaagcgat ggtcccaaaa ttgctcgttt ccaatgatac caaaaatatt gctccattt
//
LOCUS       DASRLV010000021        12763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_739882,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000021 DASRLV010000000
VERSION     DASRLV010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 12763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12763
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739882"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VFC43_00700"
     CDS             complement(<1..252)
                     /locus_tag="VFC43_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HZK29743.1"
                     /translation="MKQNDYGPIPGSSIFRGISGQKTIVMAANTRIATVAKGIFRAAK
                     DTDSAVFMELARSECDLKGGYTGLTPKDFSERIREAAKAV"
     gene            complement(271..1179)
                     /locus_tag="VFC43_00705"
     CDS             complement(271..1179)
                     /locus_tag="VFC43_00705"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HZK29744.1"
                     /translation="MILKDFLRNAGTEKNLSELILFLSSQAQDVKKGFFGNCMKTQAC
                     EQTRNTFGEEQMALDKLADGIFVSGLQKSRLVHYIATEEQDHLITIDNPKNDFGVVID
                     PLDGSSLIDVNFCIGSIIGIYPGHVLEKGITMVAALYILYGPLTILTITTGKGVHEFV
                     LDESGEFVLKEQDIKIPEGKLYAPGALRKDYLPGHARWIEHLEKEGYKLRFSGCFVAD
                     VHQILHKGGVFTYPGLKGKEKGKLRLLYEANPMGKIIHEAGGAISNGTSDILGITPDA
                     ISQVTPIYIGGKKEIRLIEKYMTEGG"
     gene            complement(1885..4476)
                     /gene="clpB"
                     /locus_tag="VFC43_00710"
     CDS             complement(1885..4476)
                     /gene="clpB"
                     /locus_tag="VFC43_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HZK29745.1"
                     /translation="MNFKNFTFKSQEAVQKATEVAAEKQNQAVETSHILKGMLLVDEN
                     VIPYLLKKLDVNLDSFIPALDKIVDSYPKVSGGEQFLSNDAAKTLQKAITLSKEYGDE
                     FVSIEHILRGILAGNDSASRLLKENGVLEKDLKNAIQELRKGETVNSQNAEDTYNALN
                     KYARNLNDLARNGKLDPVIGRDEEIRRVLQILSRRTKNNPILIGEPGVGKTAIAEGLA
                     HRIIDGDVPDNLKTKQIFSLDMGALIAGAKYKGEFEERLKSVVNEVISANGEIILFID
                     EIHTLVGAGASEGAMDAANILKPALSRGELHVIGATTLKEFQKYFEKDKALERRFQPV
                     MVNEPDTLDAISILRGIKEKYETHHNVQIKDEAIIAAVELSQRYISDRFLPDKAIDLI
                     DEAASKLRLEINSKPEELDTIERRIRQLEIEREAIKREKNQEKLKSLNEEIGNLTEER
                     NRVNAKWQSEKDLVEKIQARKNEIENLKFEAEGAERRGDLGKVAEIRYGRIPDAEKVI
                     VELKGNLTVLQKDSAMVNEEVDAEEIAEVVSRWTGIPVSRMLQSEKEKLLTLESELHR
                     RVVGQDEAIGAVSDAIRRSRAGLQDTKRPIGSFIFLGTTGVGKTELAKALAEFLFNNE
                     NSMVRIDMSEYQERHTVSRLIGAPPGYIGYEESGQLTEAVRRKPYSVVLLDEIEKAHP
                     DVFNILLQVLDDGRLTDNKGRTVDFKNTIIIMTSNIGSHIIQENMENVTEKNRDEVYD
                     STKKQVFELLKKTIRPEFLNRIDEIIMFRPLTVDEIRTVVELQLGIIQKMLDKSGIRI
                     RATKKAVQFIATMGFDPQFGARPIKRVIQKTLLNELSKMILEGKVHKDAEIVVDEKEG
                     KLVFRND"
     gene            complement(4576..6972)
                     /locus_tag="VFC43_00715"
     CDS             complement(4576..6972)
                     /locus_tag="VFC43_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HZK29746.1"
                     /translation="MADKEFTEVTVKEASHEDAGRGIARVSIDVMKKLGLVSGDVIEI
                     QGKKKAAAVVWPGFQDSGSNIISIDGNIRGNLSTGIDEKVRIRKSEAEYAKKVVIQPM
                     QPIRLVGGEQYLSRLLRGRAVYEGQTQRIDVMGNSITLVIAKVQPKGIAIVTDDTEIE
                     LKVEPYNPEEGKKELSDIHYEDIGGLGRELQLVRELIELPMKHPEIFEILGIKPPKGV
                     LFYGTPGSGKTLIAKAVANEVDAHFITLSGPEIMSKFYGDSEKALRDKFHDAEEHSPS
                     IIFIDEIDSIAPKREEVHGEVERRVVAQLLTLMDGLTGRGQVIVIAATNLLNSIDPAL
                     RRGGRFDREIEIGIPDKKGRLEIFQVHTRGVPLDNDINLEDLAETTFGFVGADIALLV
                     KEAAMNAIRNIIPLIDINKEIPSEIIQQLKLTKNDFDSARKIVLPSALREVRIEIPDV
                     PWENIAGLDEIKHTLIKIIEGRITFPQIFEKLNYTPPKGILLFGPPGTGKTLLVKGIA
                     SKRHINFISIKGPELLSKGVGDSEKHVREAFKKARQSAPCILFFDEIDSMFPKRGTIS
                     DSTHVTESVMSQFLTELDGIEELKDVFVIGATNRPDLLDPALLRPGRLEKHLFVPPPD
                     EEARIAILGIYLKDIQEILDSDVNIIDLAKKTPFFVGADLESLVREVKAGVIDELTLN
                     DNQDLDTIKISKKHFDSVLEKMKGSLDSADFEKYEQKSWDLLFTQAKRDVLYNAVHLL
                     EQIDILKRRQGADAGIIHRYEDLKDQVYWREKSFEMIQKSSDELRELVFFQLKKTPPQ
                     "
     gene            7226..8503
                     /gene="thiC"
                     /locus_tag="VFC43_00720"
     CDS             7226..8503
                     /gene="thiC"
                     /locus_tag="VFC43_00720"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HZK29747.1"
                     /translation="MSIITDARNGIVTEEMKIVAQKEGVTEDFVRRGVAQGHIVIPVS
                     PYRKVKICGIGEGLRTKVNASIGTSSDIVNIPEELEKARQAELAGADTLMELSTGGDF
                     IEIRKQVIASTTLSVGCVPLYQAFIEAAQKDGAVVNMNEDDLFRITAEQAKLGTNFMA
                     IHTGINFETVKRLRNQGRTAGLVSRGGAFMTAWMLHNEKENPLYAEFDYLLEIMKEHE
                     VTLSMGNGMRAGAVHDATDRAAIQELLINAELADKAHKDGVQVIVEGPGHVPIDEIAT
                     NVQLMKRVTNNKPFYMLGPIVTDIAPGYDDRVAAIGAAISSSLGADFICYVTPAEHLA
                     LPTPEEVFEGVISSRIAAHIGDMIKLKKTREADLQMGHARRDLDWERQYALAINPPRA
                     RAIRSERMPSDTDACTMCGDYCAIKIVNRHFKF"
     gene            8598..9233
                     /locus_tag="VFC43_00725"
     CDS             8598..9233
                     /locus_tag="VFC43_00725"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HZK29748.1"
                     /translation="MADSDLKAQKKVLRDHARHVRCTLSQAEIEEKSACICMALQNFL
                     EGIDPVMVYVSKPLEVNTRHLIEYLIAGRGRVIVPVIERETVNLRLSWLDNPSVLVES
                     TFRVHEPTGNEIPARPEDVKVAIIPVLGFDRKGNRLGYGAGYYDRFLARYPDIIRIGL
                     AFACQELSALPCEDNDIKMDLMVTENGIYHCGGNSHKDARNLLDVTHHTLK"
     gene            9252..9503
                     /locus_tag="VFC43_00730"
     CDS             9252..9503
                     /locus_tag="VFC43_00730"
                     /inference="COORDINATES: protein motif:HMM:NF024747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZK29749.1"
                     /translation="MNGDFIQINFGGFVSLSTIDWRGKSVCTVFLRGCPLRCSYCQNE
                     EIQAGDDWREIEDVFEMIKSSSPFISGVIFSGGEPTMQK"
     gene            9528..10085
                     /locus_tag="VFC43_00735"
     CDS             9528..10085
                     /locus_tag="VFC43_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29750.1"
                     /translation="MKKLDLAVGIQTNGFFPDTLESMLCENLVNKIAIDYKTTWEGYS
                     GTIGGYYPVVKENYTKNVVRSIEICKKASEKKILDEFEVVFTIFYENQEYLLEISKKI
                     GNVPLVLQQGEHKIPVLRTALSDMTDGEYIYKKQIQQEKHPPLKLDEIQQIAEKLEKP
                     VRIRTREHGEVFYEGYRGRRATRKR"
     gene            10045..10593
                     /locus_tag="VFC43_00740"
     CDS             10045..10593
                     /locus_tag="VFC43_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HZK29751.1"
                     /translation="MKVIGVVGLPASGKGEFSKIAEEMGIPVIVMGDMIRNAVKEYGL
                     DPTDANLGAIANKLRKERGMDAIAYLCIPEIQLQTAPLVLVDGIRGDAEVKLFRKTFY
                     RFTLICIDSSFENRLSRVVVRNRSDDFRTAGELRNRDERELGWGLGKALKVADVQITN
                     NGTLDEFSESVHTLLDEIRSDP"
     gene            10593..11363
                     /locus_tag="VFC43_00745"
     CDS             10593..11363
                     /locus_tag="VFC43_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZK29752.1"
                     /translation="MLKPYFSSSSKVWDDISWVYGIEEAGYAGWEIVGDGNYQLTDPV
                     CFSKIEEVIASTHLGITVHAPYGDLNLASLNDPIWRESIKQICSCIESASEITDRVTI
                     HPGYLSPLGKLMPAKVWNFQKEALRQIGKCAQEHSVLACLENMIGVKEFLCRLPEELI
                     GMTEGIEGIGMTFDFGHANTMGKVADFLAVVHKAHHIHIHDNNGMSDEHLALGEGTIP
                     WAKVGKKIADEYMGVIVIEGKSIDEAKKSLAVFRRYFL"
     gene            11360..12307
                     /gene="rnz"
                     /locus_tag="VFC43_00750"
     CDS             11360..12307
                     /gene="rnz"
                     /locus_tag="VFC43_00750"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HZK29753.1"
                     /translation="MSGETLHVYFLGTAGALPTPLRNPPCIMVRRGSDTLLFDCGEGA
                     QQQMMRSRCGFTVNAVFITHWHADHFLGLFGLVQTMSFNGRTEPLTIYGPEWVHDFVT
                     ILRQVARYNLKFSLESVEMTEGSWVRFDGYTITAFFTKHGMPSLGFVLEEDPRPGRFN
                     RDRAIELGVPPGPLFGRLQRGEVIRLGTGGETREIHPDAVMGISRPGRKIVYTGDTKA
                     VHMTIQEFAHDADLLIHDATYDESEAERGAEFFHATAAQAGEAAAVLNARVLALVHTS
                     SRYPDARDHIADAKKKFSGSIIAPDDLDMTEIAFRDEVR"
     gene            12489..>12763
                     /locus_tag="VFC43_00755"
     CDS             12489..>12763
                     /locus_tag="VFC43_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HZK29754.1"
                     /translation="MENTDVTVYRLGAGCDLSDIEEGKVYQAKVQGFATFGMFAQMND
                     RIKGLVHKSNVKAEHKERDPVLVRVRQVRPNGNIDLEEVVIQVYQVQT"
BASE COUNT         3195 a         3039 c         2991 g         3538 t
ORIGIN      
        1 tacggctttt gctgcttctc ttatcctttc tgagaaatct tttggcgtta aaccggtata
       61 cccgcctttc aggtcacatt cagaccgggc aagctccatg aataccgccg aatcagtgtc
      121 ttttgcggct ctgaaaattc ccttcgcgac cgttgcaatc cgtgtgttgg ctgccatgac
      181 aatggttttt tggccggaaa tgccacgaaa aatcgatgag ccggggattg gaccataatc
      241 gttctgtttc ataggttgat cactcctgtt ttatccccct tcggtcatat acttttcaat
      301 cagtcggatc tcttttttcc ccccgatgta gatgggagtt acctggctga tcgcgtccgg
      361 ggtaatcccg agaatatcag atgttccgtt acttatggct ccgccggcct catggatgat
      421 ctttcccatc ggatttgcct cgtataataa tctcagcttt cctttttcct tgcctttcag
      481 tccagggtag gtgaatacac cgcctttatg gaggatctgg tgtacatcgg caacaaaaca
      541 gccgctgaag cgaagtttgt agccttcctt ctcgagatgt tcaatccagc gtgcatggcc
      601 cgggaggtaa tcctttctca aagcaccggg cgcgtagagt ttcccctccg gtattttaat
      661 atcctgttcc ttcaagacga attcaccgct ctcgtctagc acgaattcat gcacaccctt
      721 tcccgtggtt atggtaagga tagtgagagg gccgtagaga atgtagaggg cagcaaccat
      781 cgttataccc ttttcaagta catgaccagg atagatcccg ataatcgatc ctatgcaaaa
      841 gttcacatca atcaatgaac taccatccag gggatcgatt acaaccccga aatcgttttt
      901 cgggttatcg atggttatca ggtgatcctg ctcctcggtt gctatatagt ggacaagtcg
      961 cgatttttgg agtcctgaaa caaatatccc gtcagctaat ttgtcaagag ccatctgctc
     1021 ttcgccaaag gtattccttg tctgctcaca tgcctgtgtc ttcatgcagt tgccaaaaaa
     1081 acccttcttc acgtcctgtg cctgtgagct taaaaagaga attaattctg aaaggttttt
     1141 ttctgttccc gcattcctga ggaaatcctt gagtatcatt cctgccacca tcctcttctt
     1201 tgaacaagac ctgcacaccg gattatttca gccgggtgcc atgatttgtg tagtcctttg
     1261 atccaaaggt ttatgagact tcccaatgcg acccgaagaa ccggatttat attgttcaat
     1321 caactccgtt tcatcggaag gtctggctta aaaatttttt ataggttctg gagaaaagtt
     1381 caaaaaacct gaacgcttac gggaatttca tccctgccac taggatctcc ccaaatgcga
     1441 taacccgtga aaggtaataa cagcattatc gcacagcgca ggagcggtca ggggggacag
     1501 ggaatgtcta gtctttttaa gcatctgtga tttcccacaa aaagtggaaa attttggttt
     1561 gatgaaggga ttatcatctc tttcttaagg tacgatgaac gccgccgccc ccgaagggac
     1621 gcccccgcgg cggatcggta actgaaaata ccattactcc tcctgtcccg ccgtgcctcg
     1681 gagaggccct gaatcggggg tctatcgcag agaaaaccga tgttcatggt aggggtgact
     1741 acttagagtt catttaggtt tctgctgaac catttcctgg tgccttcagt atctggtatc
     1801 agattcaaag tatcggcaaa aaattcctgt ggttagtatc tatcaaaacc agatacctgc
     1861 caatgaaagg attataggtc aggatcagtc attcctgaac accagttttc cctctttctc
     1921 atccaccacg atctccgcat ctttatgcac ttttccctcg aggatcattt ttgacaattc
     1981 attcagcagt gttttctgga ttacccgttt gatcggccgg gcaccgaatt gcgggtcaaa
     2041 acccatcgtg gcgataaact ggacggcttt ctttgttgcc ctgatccgta tcccgctctt
     2101 atcgagcatt ttctggataa tccccagctg gagctcgaca acagtccgga tctcgtcaac
     2161 ggtgagcggt ctgaacatga tgatctcatc gatacgattg aggaattcgg ggcggatggt
     2221 cttttttaac agttcaaaca cctgtttttt ggtgctgtcg tatacctcat cccggttttt
     2281 ttcagtgaca ttctccatgt tttcctggat gatgtgtgaa ccgatgttcg aggtcatgat
     2341 gataatggta ttcttgaaat ccaccgtccg ccccttgttg tctgtcagac ggccgtcatc
     2401 aaggacctgc agcaggatgt tgaaaacatc cgggtgggct ttttcgattt catccagcaa
     2461 taccacggaa taaggctttc tccggacagc ttcagtcagc tgcccgcttt cttcatagcc
     2521 gatatagccc ggaggtgcac cgatcagcct cgaaacggta tgccgttcct ggtattctga
     2581 catatcgatc cgcaccatac tgttctcatt attgaaaaga aattcagcca gggcttttgc
     2641 cagttccgtc tttcctacac cagttgtccc aaggaagatg aaagagccaa tcggacgttt
     2701 tgtatcctgc aaacccgccc ggctccggcg gattgcatcg gaaactgccc cgattgcctc
     2761 atcctggccc accacacgcc tgtgaagttc ggactcaagc gtcagcagct tttccttttc
     2821 actctgcagc atcctgctga cggggatccc ggtccagcgt gagacaactt cggcaatttc
     2881 ttctgcatca acttcctcgt tcaccattgc ggaatccttc tgcagcacag tcagatttcc
     2941 cttgagctcc acgataacct tttcagcatc cggtatccgg ccatagcgga tctccgctac
     3001 ttttccgaga tctcctctgc gttcggcgcc ttcggcctca aatttcaggt tctcaatctc
     3061 atttttacgg gcctggattt tttcaacaag atctttttcc gattgccact ttgcattcac
     3121 ccggtttcgt tcttcggtca ggtttccgat ctcctcattc aggctcttta acttttcctg
     3181 gttcttctca cgtttgatgg cttcccgttc gatctccagc tgacggatcc tgcgttcaat
     3241 cgtgtccagt tcttcgggtt ttgagttgat ctcaaggcgg agttttgatg cagcctcatc
     3301 gatcaggtcg attgccttgt caggaaggaa acggtcggat atatatcgtt gcgagagttc
     3361 aacggcggca atgatggcct cgtctttgat ttgaacatta tgatgtgttt cgtatttctc
     3421 tttgattccc cgaaggatgg aaatggcatc cagcgtatcc ggttcattca ccatcaccgg
     3481 ctggaaacgc cgctccagtg ctttgtcctt ttcaaaatat ttctggaatt cctttaaggt
     3541 agttgcgcca atgacgtgca gttcgcctcg tgaaagcgcg ggttttaaaa tattggcggc
     3601 gtccattgcg ccctcgctgg cgccggcccc gaccagcgta tgaatctcgt caataaagag
     3661 gataatctcg ccatttgcgg atatcacttc attgaccaca ctttttaatc tttcttcaaa
     3721 ttctcccttg tatttagccc cggcgatcag ggcacccata tcgagagaaa agatttgttt
     3781 ggttttgagg ttatccggta catccccgtc aatgatccgg tgagcaagac cttcggcaat
     3841 ggctgtcttt ccaacacctg gttcgccgat gaggatcggg ttgtttttcg tcctccttga
     3901 aaggatctgc agtaccctgc ggatctcttc gtcgcgaccg ataaccggat caagtttccc
     3961 gttacgggca agatcattta agttccgggc atatttgttc agggcattat aggtatcttc
     4021 tgcgttctgg ctgttcacgg tttcaccttt ccgtaactcc tgtatggcat tttttaaatc
     4081 tttctccaaa accccgtttt ccttgaggag ccttgaagcg ctgtcgttgc ctgcaaggat
     4141 tcccctgaga atgtgctcga tggagacaaa ttcatccccg tattccttgg atagtgtgat
     4201 tgctttctgg agggttttag cagcatcatt tgagaggaac tgctccccgc cactgacttt
     4261 tggatatgaa tcgacgatct tgtcaagggc agggatgaaa ctatcgaggt tcacatccag
     4321 tttctttaag aggtatggga taacgttctc gtctaccaga agcatcccct tcagtatatg
     4381 cgaagtctca acagcctggt tctgtttttc cgctgcaacc tctgttgcct tctgaacggc
     4441 ttcctgtgac ttgaaggtga aatttttgaa attcatggta ttgaccatac ctggtagttc
     4501 cccatttgag atttaccata agttagtatg aaattatata taaatatgtg gttgtgacaa
     4561 caaaagaaca aagaattact gagggggagt tttcttcaat tgaaaaaaga ccagttcacg
     4621 cagctcatct gaacttttct gaatcatttc aaatgatttc tctctccagt acacctggtc
     4681 cttgagatcc tcgtaacgat gaatgatccc ggcatctgcc ccttgtcttc tcttcagaat
     4741 atcaatttgt tcaagaagat gtacagcatt gtaaaggaca tcgcgtttag cctgggtaaa
     4801 taacagatcc catgatttct gctcgtactt ttcaaaatct gcactatcaa gggatccttt
     4861 cattttttca agcacactgt caaaatgttt ctttgagatt tttatggtat ccaggtcctg
     4921 attgtcgtta agggtcagtt catcaattac ccctgccttg acttcacgca ccagagattc
     4981 caggtcagcc ccgacaaaaa aaggcgtctt ttttgccaga tcgatgatat tgacatcgct
     5041 gtcaaggatc tcttgtatat ctttgagata tattcccaga attgcaattc ttgcttcttc
     5101 gtctggaggt gggacgaata aatgtttttc cagcctgcct ggtcgcagga gtgccggatc
     5161 aagtaaatca ggacggttgg ttgctccgat aacgaagaca tcctttaatt cctcaattcc
     5221 atccagttct gtcaggaact ggctcatcac gctctcggta acatgcgttg aatcactaat
     5281 tgtccctctt ttgggaaaca ttgaatcgat ttcatcaaaa aataagatgc agggtgctga
     5341 ctgacgtgct tttttgaagg cctcacgcac atgcttttct gaatcgccaa cgcctttgga
     5401 gaggagttcg ggacctttta ttgatataaa attaatatgc cgcttgctgg caataccttt
     5461 gacgaggagg gtctttcccg tacccggtgg accaaagagt aaaattcctt tgggtggtgt
     5521 gtagtttaat ttttcaaaga tctgcgggaa ggttatcctg ccttcgatta ttttgatcag
     5581 ggtatgcttg atttcatcca gacctgcaat gttttcccag ggaacgtcag gaatttcgat
     5641 acggacttcg cgtaatgcac tgggcaggac aatttttctg gccgaatcaa aatcattttt
     5701 tgtcagtttc aactgctgga taatctcact ggggatttct ttattgatat caatcagagg
     5761 aatgatattc ctgatggcat tcattgccgc ttcttttact aacagtgcaa tgtcagcccc
     5821 gacaaaccca aacgttgttt ctgcaagatc ctcaaggttg atatcattat ccaggggaac
     5881 tccccgtgta tggacctgga aaatttcaag acgtcccttt ttatcaggaa ttccaatctc
     5941 gatctcccga tcaaatctcc cgccgcgccg tagtgcaggg tcaatggagt tgagcagatt
     6001 ggttgctgcg atgacaatca cctgaccccg gccggtcaga ccgtccataa gggtaagaag
     6061 ttgtgctacc acacgtcgtt caacttcccc atgcacctct tctctttttg gagctatcga
     6121 atcgatttca tcgatgaaga taatggaggg ggaatgctct tccgcgtcat gaaatttatc
     6181 ccgcaatgcc ttctcgctgt caccataaaa tttgctcatg atctcgggac cggacagggt
     6241 gataaaatgg gcgtccactt catttgcaac ggcttttgcg atcagcgtct tgccagaccc
     6301 cggcgttcca tagaagagga cccccttggg aggttttatt cccaaaattt caaaaatttc
     6361 cggatgtttc atggggagtt cgatcaactc cctgaccagc tgcagctccc gcccaaggcc
     6421 acctatgtct tcgtagtgga tatcagaaag ttcctttttg ccctcttcag gattataggg
     6481 ttccactttg agttctatct cagtatcatc agtaacaatg gcgattcctt ttggctggac
     6541 ttttgcgatg acaagggtga tggaatttcc catcacatcg attctctggg tctgcccttc
     6601 atacacagcc cttccccgca ggagtctcga gagatactgt tccccgccaa ccaggcggat
     6661 aggttgcatg ggctggataa ctactttttt agcatattca gcttcggact tccggatcct
     6721 gactttttca tctataccgg tgctcaggtt accacggata ttcccgtcta tactgataat
     6781 gttggaaccg ctgtcctgga agccgggcca gactaccgca gcagctttct ttttgccctg
     6841 gatctcgatt acatcaccgg ataccaaccc gagctttttc attacgtcaa tgcttaccct
     6901 cgcaatgccc ctgccggcat cctcatgcga agcttccttg accgttactt cggtaaactc
     6961 tttatcagcc attgcaaaat atcctgaatt attttggaat taccctaagt tcgggttcaa
     7021 ttctatttat ttagtgtttt taaggctaaa attatgtaac tactctccct gaaaatttaa
     7081 atacctatgt caggtatgac tgataagaaa ttcaaaaaaa tttaccttaa tccaaatcaa
     7141 ctttacttgt taacttaaca agccagattt agtccagaat ttaaaaaccg agtctttatc
     7201 ttgtctttac ggtaacgtgt aagatatgag tattatcaca gatgcaagaa acggcatcgt
     7261 aacagaagag atgaaaatag ttgcacagaa ggaaggggtc actgaagatt tcgtccgtcg
     7321 cggtgttgca cagggacata ttgtgattcc cgtatctccc tatcgtaaag taaagatttg
     7381 cgggataggt gaagggctcc gcacaaaagt gaacgcttct attggcacat catcggatat
     7441 tgtaaatatt cccgaggaac tggaaaaagc acgtcaggca gagctcgcag gtgccgatac
     7501 gctgatggag ctttcgaccg gcggagattt tattgagatc cgcaaacagg tgattgccag
     7561 cacaaccctg tcggttggct gtgttcctct gtaccaggct tttatcgaag cagcacaaaa
     7621 ggatggcgct gtcgtaaaca tgaatgagga tgacctgttc cggatcaccg cagaacaggc
     7681 aaaactcgga acaaacttca tggccatcca taccggtatc aactttgaaa cagtaaaacg
     7741 tctccggaac caggggagaa ccgcgggact ggtatcacgg ggcggtgcat tcatgactgc
     7801 atggatgctc cataatgaaa aggaaaaccc gctctatgcg gaatttgatt acctgcttga
     7861 gatcatgaaa gagcacgaag tcaccctttc catgggaaac gggatgcggg ctggcgcagt
     7921 ccatgatgca actgaccggg ctgcaataca ggaacttttg attaacgctg aacttgcgga
     7981 taaagcacat aaggacgggg tccaggtgat tgttgaggga cccgggcatg taccaatcga
     8041 cgagattgcc accaacgtgc aactcatgaa acgggtgacg aacaacaaac ccttttacat
     8101 gctcgggcct attgtgactg acattgcccc cggctatgac gatcgtgttg ccgccattgg
     8161 tgcagcgatc tcttcatcgc ttggtgcaga ttttatctgt tacgtaaccc ctgccgagca
     8221 ccttgccctc cccactcctg aagaagtttt tgagggagtt atcagttcac ggatcgccgc
     8281 ccatatcggt gatatgataa aactcaaaaa gacccgggaa gcagatctcc agatgggaca
     8341 tgcccgccgc gatcttgact gggaacgcca gtatgccctt gcgattaatc caccccgtgc
     8401 ccgcgcaatc cggagtgaac ggatgccgtc agataccgat gcctgcacca tgtgtgggga
     8461 ttactgtgca ataaaaattg taaaccgcca ttttaaattt taatccccac catacttttt
     8521 gatacgcatt ttccttcctt catcccggat agtacctgga tcaatcggac aggtgcgttc
     8581 attgatgaac tgatatcatg gcagactccg atttaaaggc ccaaaaaaaa gttctccgcg
     8641 atcatgcacg acacgtacgg tgcacattat cccaagccga gatcgaagaa aaaagtgcat
     8701 gtatctgcat ggccttacaa aattttcttg agggaatcga ccccgtcatg gtgtatgtat
     8761 caaaacccct cgaggtaaat acgaggcacc ttatcgaata cctgatcgcg ggaaggggac
     8821 gggtcattgt cccggtaatc gaaagagaga ccgtgaacct gcgcttgtcc tggcttgata
     8881 acccttcggt gcttgttgag agtacctttc gtgttcacga accgacaggc aatgaaattc
     8941 ctgcaagacc cgaagatgtc aaagttgcga ttataccggt acttggattt gaccggaagg
     9001 gaaaccgcct cggttatggc gccgggtact atgaccgttt tttagccagg tatccggata
     9061 tcataaggat cgggctcgct tttgcctgcc aggaattatc cgcccttccc tgcgaagaca
     9121 atgatataaa aatggacctg atggtgaccg aaaacggaat ttatcattgc ggcggaaatt
     9181 cacacaagga tgccagaaat ctcctagatg taactcacca tacactcaaa taacctcgta
     9241 acaaaataca tatgaacggt gatttcattc agataaattt cgggggattt gtctcattat
     9301 ctacgattga ctggcggggc aaatcggtgt gtacagtttt tttacggggg tgtccgctcc
     9361 gctgttcata ctgtcagaat gaggaaatac aggcagggga tgactggcgg gaaatagagg
     9421 atgtttttga gatgataaag tcgtcttcac catttatcag cggggtaatt ttttcgggag
     9481 gagagccaac gatgcaaaag taaggactga ctgcgcttgc ccggtatgtg aaaaaactgg
     9541 acctggcagt cggaatacag acaaacgggt tctttcctga tacacttgag tcgatgctat
     9601 gtgaaaatct ggtaaataaa atagcgatcg attataaaac aacctgggaa ggatattccg
     9661 gtacgatcgg tgggtattac ccggtagtca aagaaaatta tacaaaaaat gttgtacggt
     9721 cgattgaaat ctgtaaaaaa gcgtcagaaa agaaaatact ggatgagttt gaagtggttt
     9781 ttaccatttt ttatgaaaac caggaatatt tactggaaat ttccaaaaaa atcggaaatg
     9841 ttcctctggt cctccagcag ggtgagcata agatcccggt attgcgcact gcgctgtctg
     9901 acatgaccga tggggaatat atttacaaaa aacaaataca acaggaaaag catccccccc
     9961 tgaagctgga tgaaatacaa cagattgctg aaaaactgga aaaaccagta cgtatccgca
    10021 cacgcgaaca tggagaggtt ttttatgaag gttatcgggg tcgtcgggct acccgcaagc
    10081 ggtaaaggtg aattttcaaa gattgcagaa gagatgggaa tccccgtcat agttatgggg
    10141 gatatgatcc ggaatgcggt gaaagaatac gggcttgacc ccaccgatgc gaaccttgga
    10201 gcgatagcga acaaactcag gaaagaacgg ggtatggatg caattgcgta tctctgtatc
    10261 ccggaaatac aactccagac cgctccgctt gtcctggtcg acggtatccg gggggatgct
    10321 gaagtgaaac ttttccgtaa gactttttac cggtttaccc tcatatgcat cgattcctcg
    10381 tttgagaacc ggttgtcaag ggttgttgta cggaaccgtt cggatgattt caggacagcg
    10441 ggtgaactcc gcaaccggga cgaacgcgag ctcggctggg gtcttggcaa ggcattgaaa
    10501 gtggccgatg tgcagataac gaacaacggc acactcgatg aattttcgga atctgtgcat
    10561 acactcctcg atgagatccg gagcgatcct taatgcttaa accctatttt tcatcatcat
    10621 cgaaagtctg ggatgatatt tcctgggtat atggaataga ggaggcaggg tatgccggct
    10681 gggagatcgt cggtgatggc aattaccagc tcacggatcc tgtgtgtttt tcaaaaattg
    10741 aggaggtaat cgcaagtacg caccttggta ttacggtaca tgcaccctac ggcgacctca
    10801 accttgcctc cttaaatgac cctatatggc gcgaatcgat aaagcagatc tgttcctgta
    10861 ttgaatctgc atccgaaata acagaccggg ttacgattca tcccgggtac ctttctcccc
    10921 tgggaaaact gatgcccgca aaagtctgga atttccagaa agaagcctta cggcagatcg
    10981 gaaaatgtgc acaggaacat tcagtgcttg cctgccttga aaatatgatc ggggtaaaag
    11041 aattcctgtg ccggttgcca gaagagctta tcggtatgac tgagggaatt gagggaatcg
    11101 gcatgacgtt tgatttcggg cacgcgaaca cgatgggaaa agttgcggat tttcttgcag
    11161 tggtacataa agctcatcat atccatattc atgataataa tggcatgtcc gatgaacatc
    11221 tggcccttgg tgaaggcact attccgtggg ctaaggtcgg gaaaaaaatt gccgatgagt
    11281 acatgggggt tatcgttatt gaaggaaaat ccattgatga ggcaaagaaa agtctcgcag
    11341 tattcaggag atatttttta tgagcggtga gaccctgcat gtctatttcc tgggtaccgc
    11401 cggggcgctc ccgactcccc tgcgtaaccc cccctgcatt atggtccgca ggggttctga
    11461 tacccttctc tttgactgtg gcgagggcgc ccagcagcag atgatgcgtt ctcggtgtgg
    11521 ttttacggtc aatgctgttt ttatcactca ctggcatgcc gatcacttcc ttggtctttt
    11581 tggtcttgtc cagaccatgt cttttaacgg aaggaccgaa ccactcacca tttatggtcc
    11641 tgagtgggtg cacgattttg tcaccatcct ccgtcaggtc gcccgttata atctcaaatt
    11701 ctcccttgaa tccgttgaga tgaccgaggg gtcctgggta cgttttgacg ggtataccat
    11761 tacggctttt ttcaccaagc acggaatgcc gtcgcttggg tttgtccttg aagaagatcc
    11821 ccgcccgggc agattcaacc gcgatcgggc gatcgagctg ggtgtaccac cgggcccact
    11881 cttcggccga ctccagcggg gcgaggttat ccgcctggga accggtggcg agacccggga
    11941 aattcatcct gatgcggtga tggggatctc aagacccggt cgcaaaatcg tgtataccgg
    12001 tgataccaaa gccgtccata tgactattca ggagtttgcc catgatgctg atctcctgat
    12061 ccatgatgcg acgtatgatg agtcagaagc ggaacgggga gcagagtttt ttcatgcaac
    12121 cgctgcgcag gcaggagaag ctgcagcagt gctcaatgca cgtgtccttg cgttagtcca
    12181 taccagttca cgctatccgg atgccaggga ccatatcgcc gatgcgaaga aaaaattttc
    12241 cggttccatt attgctccgg acgatctgga tatgacagaa atcgcgtttc gggatgaggt
    12301 acgataaaga atactaaaaa aataagaaat ccaaaagaaa attaccgcca gatgtcagat
    12361 acaatgaatt ttaacatagc cattcctaat aagtaagcat cagctttgtt tcgttatgca
    12421 ggttgtaatt tgataaaaaa caacaatgca gggaataagt acgcttcgct gtgtgtggtg
    12481 aaaaaacaat ggaaaatact gatgtaacag tgtatcggct gggtgccgga tgtgacctgt
    12541 ccgatatcga ggaaggaaag gtataccagg caaaagtaca gggttttgca accttcggga
    12601 tgtttgccca gatgaatgac cggatcaagg gtcttgtgca taaaagcaat gtaaaagctg
    12661 aacataagga acgcgatccg gtccttgtac gggtacggca ggtacgcccg aatggtaata
    12721 tcgacctcga agaagtggtc atacaggtct accaggtcca gac
//
LOCUS       DASRLV010000022         2627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_740259,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000022 DASRLV010000000
VERSION     DASRLV010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740259"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..941
                     /locus_tag="VFC43_00760"
     CDS             321..941
                     /locus_tag="VFC43_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxymethylpterin diphosphokinase MptE-like
                     protein"
                     /protein_id="HZK29755.1"
                     /translation="MRFEVWEPYYRDILEYFGFDRAQDEEAARLLGTLMTRDNILSLT
                     SLTCGNDITVCGNAPCLKKELDKISGVVFAADAAAEVLDAHGIIPDAVFTDLDGADDR
                     LLELNESGTIVVIHAHGDNMPLLRSWVPRFKGPVVATTQSTPLKNVYNFGGFSDGDRA
                     VFAADELGAAHITIIGFDLDDTNVDPMKRGKLFWARKLLAAIGHVC"
     gene            931..2574
                     /locus_tag="VFC43_00765"
     CDS             931..2574
                     /locus_tag="VFC43_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZK29756.1"
                     /translation="MSAEACILDGYVDEPACLGVPPYISPYIRTVAGSLIGHEFTVRY
                     RTIDQLRNDPHTINLLDHADLLVMIAGVTVPGKYLGGTPATLNEIQQVGHMIRNPKKL
                     IGGPIGFGYAHEGGQRAIKQVISGFDALLTGEPAVALDNYLTGNGPAGVLDYHRTDPW
                     SIRGSEIIVQHPDFPYVMCELETARGCSHGASGGCSFCTEPFYGSPKYRSTEGIAQEV
                     AALHTHGARHFRVGRQPDILAYGAGAGEYPAPRPDLIGNLFASIRAAAPELKTLHIDN
                     TNPATIAKHEDESRGALREIIRYHTPGDVAAFGMETADPAVVQKNNLKAGTEDVLRAI
                     RIVNEEGGMRRDNVPELLPGLNFVCGLAGETEKTFDLNERFLELVRDEGLLVRRVNIR
                     QVMPFEGTPAYTTNTLGKHDRRFRAFKEFVRQKIDLPMLQRVYPVGTVLREVQIEVSG
                     DLSFGRQMGSYPILVGIPVRLARRTIIDAVVVDWGMRSVTALPVPVEINTLSASALKW
                     LPGVGKKKTVAVIAKRPFKTLEEYQKIAGISSIDPLLKF"
BASE COUNT          636 a          642 c          722 g          627 t
ORIGIN      
        1 ggcagcagcc cagcttcccc tccactattt tggaaaagtg gagaacaaag cccagcttct
       61 tgagtttgtt atcgagccca tattgcaaaa attcaagaat ctcgtaggcc tggaaaatga
      121 aggtaaaaag aacggttcat aagttcagaa ataccggatt acccggccgt tatgggttcc
      181 caatcttggg atagccggag ctataccggg aaaagtcagg tagtagtgtt ttccccttgc
      241 aacagatgaa aagggcggga tgatgaacat cagatcctgg aaatctcagg tatcatttga
      301 agaaattatt ggggtaaatg atgagatttg aagtctggga gccttattac cgcgatatcc
      361 ttgaatattt cgggtttgat cgggcacagg acgaagaggc agcccggttg cttgggacac
      421 tgatgacccg ggataatatc ctctccctta catctcttac ctgcgggaat gatatcaccg
      481 tctgtggtaa tgcgccctgc ctaaaaaaag aacttgataa aatcagcgga gtagtttttg
      541 cagccgatgc tgcagcggaa gtacttgatg cacacggtat tattccggat gcagttttca
      601 ccgatcttga tggtgctgat gaccggctcc ttgagctgaa tgaatcagga acaattgtgg
      661 taatccatgc acacggggat aatatgcccc tgctccgttc ctgggtgccc cgtttcaaag
      721 gccctgtggt tgccacaacg cagagtactc cgctcaaaaa cgtctataat ttcgggggtt
      781 tttctgatgg cgaccgggca gtttttgctg ccgatgaact gggggcggct catataacca
      841 tcatcgggtt tgacctcgat gataccaatg tagatccgat gaagcggggt aaactgttct
      901 gggcacggaa gctcctggcg gcgatcggcc atgtctgctg aggcatgtat ccttgacggc
      961 tatgtggatg aacctgcatg tcttggggtc cccccgtata tttcccccta tatccgcacg
     1021 gttgccggct cattgatcgg acatgagttt actgtgcgtt accggaccat tgaccagctc
     1081 cgcaatgatc cgcacacgat caacctgctg gaccatgccg acctgctggt catgatcgcg
     1141 ggtgtaacgg tcccgggaaa ataccttggt ggcacgcctg cgaccctcaa tgagatccag
     1201 caggtaggac acatgatccg caatcccaaa aagctgattg ggggtccgat aggattcggg
     1261 tatgcccatg aaggcggtca gcgggcgata aaacaggtaa tttccggttt tgatgccctg
     1321 ctgacgggtg aacctgccgt agcacttgat aactatctta ccggtaatgg accggctggt
     1381 gtacttgatt atcaccggac agatccgtgg agtatccggg gcagcgaaat tatcgtgcag
     1441 cacccggatt ttccgtatgt gatgtgtgag ctggagacgg cacggggctg ttcccatggt
     1501 gcatccggag gctgttcatt ctgcaccgaa cccttctatg gaagtccgaa atatcgcagt
     1561 accgaaggca ttgctcagga agttgctgcc ctgcacactc acggggcgag gcatttcagg
     1621 gttgggcggc agccggacat cctcgcgtat ggcgccggag ccggggaata tcctgcaccg
     1681 cgcccggatc tcatcggaaa cctgtttgca tcgatccggg ccgcagcacc tgaactaaaa
     1741 accctgcaca ttgataatac gaatcctgca accatagcga aacatgaaga tgagagccgc
     1801 ggagcattgc gcgaaattat ccggtatcat acccccgggg atgttgccgc gttcggtatg
     1861 gaaactgcgg atcccgcagt ggtccagaaa aacaatctca aggctggtac agaggatgtc
     1921 ttacgggcaa tccggatcgt gaatgaagaa ggcgggatgc gcagggataa tgtgcctgaa
     1981 ttgcttcccg ggctcaattt tgtgtgcggt cttgcaggag agacagaaaa gacgttcgat
     2041 ctcaacgaac ggtttcttga acttgtacga gacgagggac tccttgtcag gcgggtaaat
     2101 atacggcagg tcatgccgtt tgaaggcaca cccgcctaca ccactaatac tttaggaaaa
     2161 cacgaccgcc ggttccgggc attcaaggaa ttcgtacggc agaaaattga tctccctatg
     2221 ctccagcggg tctatcctgt tggaaccgtg ctccgggaag tccagattga agtttcgggt
     2281 gatctctcgt ttggtcgcca gatggggtca taccccattc tggttggtat ccctgtacgg
     2341 cttgccagac ggactattat cgatgcagtc gtggttgact gggggatgcg atcggttacg
     2401 gctttaccgg ttcctgtgga gatcaatacg ctctctgcat ccgcactgaa atggctcccg
     2461 ggagtaggaa agaaaaaaac tgtggcagta atcgcaaagc gcccctttaa aacattagag
     2521 gaataccaaa agattgccgg gatatccagc atcgatccgc tgctgaaatt ctaaatctgc
     2581 ttcagttcgg tcactttgat gatatcggtc cgggtaacaa tccccag
//
LOCUS       DASRLV010000023         5984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_8243, whole
            genome shotgun sequence.
ACCESSION   DASRLV010000023 DASRLV010000000
VERSION     DASRLV010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8243"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..590)
                     /locus_tag="VFC43_00770"
     CDS             complement(174..590)
                     /locus_tag="VFC43_00770"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HZK29757.1"
                     /translation="MKQGIVLLAITIILIAVCGCTQTPATTPNQTNPTTPAITTESQT
                     VVTTPTQQKTPTVSDNTVRIKNLAFDRVNISVKVGSTVRWVNLDSVPHRILFEDGADS
                     TVLAGSQSWSRKFDEAGTYNYACTIHPTMQGTVIVD"
     gene            complement(671..1375)
                     /locus_tag="VFC43_00775"
     CDS             complement(671..1375)
                     /locus_tag="VFC43_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29758.1"
                     /translation="MIGKLDARFVNSKDSEEISLGGRNWSMVKCDEGHNIVVVVPSGS
                     DTSRIFWTSSGESGFSPLVCRMVQRICSSRRSALELPEHEHEVIQPVLQKTPAGFGKK
                     GLHIIERKGSKGKEVLVYSFSGSMFNGLLCVLLQDRIGGRVQVKYNDFIIRVYRAGKD
                     GLRECVMHALKEIQNMGRDEIRKVLPMPRSDEWKFSRALPSNLLSDLSLSDHYHVEEF
                     MKRIKSEQFIALNSDQ"
     gene            complement(1379..2854)
                     /locus_tag="VFC43_00780"
     CDS             complement(1379..2854)
                     /locus_tag="VFC43_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZK29759.1"
                     /translation="MIPPGKNITRQAPRLTESVSSVFSTLHESLQQVLSQRLDWTELR
                     EVQEQTYSAVVKGSDVLVIAPTAGGKSEAALIPVIDDILKNGRTGISCLYISPLKALI
                     NDQEERFRTFCTPTSLSVMKWHGDVPKGDRSWADGEPPQFLMITPESLEVLLQEKKLL
                     PDLRHIRTIIIDELHAFVESERGVHLKVLLNRMDHITKRHVQRIGLSATAGNPEEMLE
                     WLSDNRQKAELVAIPSSPKEKQFLFIVEPEEADRINALVRIVDQKKALVFVNSRSVAE
                     KLMKAAAGRIRNLHIHHSSLSPETKKSSEAAFSSTEGACIICTSTLELGIDIGDLDVV
                     VQVGPPNSVSSFLQRMGRTGRRGKSAFVAWVLQNPCELLCSIAIIECAIKKEVEKLVP
                     PLKPYNVLLQQIFLYLHNHPCTSRRNLAASVLSYPVFKDIRPDVLDQIISHLIKNEYV
                     TTDGEMVMLGTEAERVFGRSNWKDLYSVISGGGEYRAMTPD"
     gene            3170..4744
                     /locus_tag="VFC43_00785"
     CDS             3170..4744
                     /locus_tag="VFC43_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HZK29760.1"
                     /translation="MAVDPVTTAAFVLVYIAITLIIGYIGYRQTKNSDDYLVAGRSSH
                     PIVIALSYGATFISTSAIVGFGGVAANLGMGLIWLTVLNIGVGILIAFVVFGKKTREI
                     GQRLSAVTYPDLMGKIYKSALMKYISGIIIVVAMPLYTAAILIGGARFLEPTLNIPYN
                     YALLIFAAVVALYVVFGGLIAVMYTDAFQGLIMLVGMTALLVLTYIYLGGVTAAHTAL
                     TAMSDLVPSSLAAQGMTGWTSMPSLGSPIWWTLISTLVLGVGIGVLAQPQLVVRFMTA
                     KDNKSLNRAVAMGAPWLLMMTGVAFTVGALTNVYFYQQTGKIAIDAAGGNVDAIIPAY
                     INLAMPDIFVIIFMLTLLSAAMSTLSGLYHAMGTALICDIWGRGRACALSMKANQAGV
                     IIMMVASVALAFVMPTNIIARATAMFMGLCAAAFLPAFAAGIYAKNPSTKAALWSMIS
                     GSICWFLWTAFIHTAESKPLGLCQFIFGKPALLPLPWTVIDPIIIALPVSAAIMILMQ
                     WQSGSKNKSISPSISE"
     gene            4836..5159
                     /locus_tag="VFC43_00790"
     CDS             4836..5159
                     /locus_tag="VFC43_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012108003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HZK29761.1"
                     /translation="MELAQKKCSTYRIGSPPLTRKEIFELMGELPGWSLDNGHLIRIF
                     EFDDSGQCVGFFNEVVALSTEEGHIPDICMKESRFVEISYYTYPAGGLTLNDFIMAAK
                     LNTRS"
     gene            complement(5166..5567)
                     /gene="trxA"
                     /locus_tag="VFC43_00795"
     CDS             complement(5166..5567)
                     /gene="trxA"
                     /locus_tag="VFC43_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HZK29762.1"
                     /translation="MDDEISRIREKRLQELKDRMKHTVKAEISEVDEMHFQEFIKTHQ
                     FAVLDFWAEWCAPCRRVGIVLEDLSKEFSGKVTFGKCNTEENRALALKFNITAIPSIM
                     LFSHGQLVDRIIGAYPKEALREKIVKKFNLK"
     gene            5682..5756
                     /locus_tag="VFC43_00800"
     tRNA            5682..5756
                     /locus_tag="VFC43_00800"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5717..5719,aa:Met,seq:cat)
BASE COUNT         1467 a         1448 c         1361 g         1708 t
ORIGIN      
        1 tttttttgtg tttatccgga tagggtattt ttatccggaa aataaggaaa cgtgattttc
       61 atttgatggg attttttttg attggctttt tcaaacaaaa atctgattga tattcttccg
      121 attaccgggt aaacatttaa aaaaaactat tggatactat tcaccaacat cagtcaatct
      181 acaattactg ttccctgcat ggtcgggtga atagtgcagg cgtagttgta tgtgcctgcc
      241 tcgtcaaatt tcctgctcca tgattgcgaa ccggcgagca cggttgaatc cgctccatct
      301 tcaaataata tcctgtgggg tactgaatcc aggttcaccc atctgaccgt ggatccgact
      361 ttcacgctga tatttacccg atcgaaagcc agatttttaa tcctgacagt attatccgat
      421 acggtcggcg tcttctgttg cgtcggcgtc gtaacaaccg tttgcgattc cgtcgtgatc
      481 gcaggggtag tcggatttgt ctgattaggg gtagtcgcag gtgtctgggt gcatccgcag
      541 actgcaatca ggatgatggt aatggcaaga agaactatac cttgtttcat agattaatct
      601 ccactgtttt ctatataata ccggatccct ttggcttttt aatttgaatg gacatcgtat
      661 tcttctgaat tcactggtcg gaattcagcg ctataaattg ttcactcttt atccttttca
      721 tgaactcttc aacgtgatag tggtcagata aggacaggtc tgaaagcaga tttgatggaa
      781 gagcgcggga aaatttccat tcatctgacc ggggcattgg gagaactttc ctgatttcgt
      841 cccttcccat attctggatt tctttcaatg catgcatgac acactccctc aacccatctt
      901 ttcctgcccg gtacaccctg ataataaaat cattatactt tacctgcacc ctgccaccga
      961 ttcgatcctg aagcaggaca cacagcaacc cgttgaacat gcttccggaa aaagagtata
     1021 caagcacctc ttttcctttt gatccttttc gttcgataat atgcagccct ttcttgccaa
     1081 agcctgcggg agtcttttgc agtacaggct ggataacctc atgttcgtgt tcagggagtt
     1141 cgagtgctga tctcctagaa gaacatatcc tttgtaccat acggcacaca agtggcgaaa
     1201 acccgctttc accgctgctg gtccagaaga tgcgggaagt atctgaccca ctcggtacca
     1261 cgactacaat attatgtcct tcatcacact tcaccatgga ccagttgcgt ccaccgagcg
     1321 agatctcttc tgagtctttg ctgttcacga accgtgcatc cagctttcct atcatctctc
     1381 aatccggcgt catggcccga tactctccac ccccgctgat aactgaatag agatccttcc
     1441 agtttgaccg cccgaacacc cgctcagcct cggtacccag catcaccatc tctccgtcag
     1501 tggttacgta ctcattttta atgaggtgcg aaataatctg atccagaaca tcgggtctga
     1561 tatccttaaa gaccggatag gagagcactg atgctgcaag attcctgcgg cttgtacagg
     1621 ggtgattatg cagatacagg aaaatctgct ggagcaggac gttatagggt tttaacgggg
     1681 ggacgagttt ctctacttct tttttgatag cacattctat aattgcaatg ctgcaaagca
     1741 gttcacacgg gttctggaga acccatgcaa caaaagctga ttttcccctg cggccggttc
     1801 gtcccatgcg ctgaagaaaa gaagaaaccg aatttggcgg accaacctgg accacaacat
     1861 caaggtcacc aatatcgatg ccgagttcaa gggtgctggt gcagatgata caggcaccct
     1921 ccgttgagga gaatgctgct tctgaggatt tttttgtctc tggcgagagc gatgaatggt
     1981 gaatgtgaag atttctaatc cgtccggcag ccgccttcat gagtttttct gccacgctcc
     2041 tgctgttaac aaaaacaagg gctttctttt gatccacaat ccgcacaaga gcattgatcc
     2101 ggtccgcttc ttcaggttcc acaataaaca ggaactgttt ctctttggga gaggatggaa
     2161 tcgcaacgag ttccgccttt tgccggttat cagacaacca ttccagcatc tcttcgggat
     2221 ttcccgctgt tgctgaaagt ccaatccgct ggacatgtct ttttgtgata tgatccatcc
     2281 ggttgagcag gaccttaaga tgcacgcccc gttctgactc aacaaacgca tggagttcat
     2341 cgatgattat ggtcctgata tgcctgagat ccgggagcag ctttttttcc tggagcagaa
     2401 cctcaagcga ttcaggtgtg atcattaaaa actggggagg ttcgccatcc gcccatgagc
     2461 ggtctccctt tgggacgtca ccatgccatt tcataacgga caaggaagtc ggcgtgcaaa
     2521 aagtcctgaa ccgttcttcc tgatcattaa tcagtgcttt caagggagag atgtaaaggc
     2581 aggaaatacc agtcctgcca tttttcagga tatcatcaat aacagggatg agtgctgcct
     2641 ccgacttacc tccggcagtg ggcgctatca caagcacatc acttccctta actactgctg
     2701 aataggtctg ctcctgcact tctctgagtt cagtccagtc cagcctttgc gagagcacct
     2761 gctggagtga ttcgtgaagg gttgaaaata ctgatgacac actttcagtt aaacgtggtg
     2821 cctgacgcgt tatgttcttt ccgggtggaa tcataaaaac tggtggttaa aaagagatat
     2881 gcaaacacat atatacaaaa attttcagaa aagaagttgg taaattatgc tgtcactatg
     2941 ggtttgtaat tccgttttta catcaaaccc attcttcttt aactttagat aggggagccg
     3001 taacaccggt tccccgtgta atgttgtgat tccgagttta ccagaggttt ttgtatgctt
     3061 tttggaattc ctgacctgca gatagtaatt ggctatggtc ttgccctggg cctggtgttg
     3121 gcctgcatag tgtatggatg gttatactgg gacgataacg gggacgaaca tggcagttga
     3181 ccctgtcacc actgcggcgt ttgtattggt gtatatagcc atcacactca tcatcggtta
     3241 tataggttac cggcagacaa agaacagtga tgactacctt gttgccggac ggagcagtca
     3301 tcccatcgtg attgcacttt cttatggtgc aacttttatc agcacatctg ccatagtcgg
     3361 cttcggtgga gttgcagcta atcttggcat gggactgata tggctcaccg ttctgaatat
     3421 tggtgttggt atcctgatcg cattcgtggt attcggaaaa aaaacgcggg agatcgggca
     3481 gcggttgagt gctgtcacct atccggacct tatgggaaag atctacaaat cagcgcttat
     3541 gaagtacatc agcgggataa ttatcgttgt tgcaatgccg ctgtataccg ctgcaatttt
     3601 gattggcgga gcccgttttc ttgaacctac gttaaatatc ccttataatt acgcgttgct
     3661 tatctttgcc gccgtagttg ccctctatgt tgttttcggc gggttgatcg cggtcatgta
     3721 tactgatgcc ttccaggggc tgatcatgct ggtgggtatg actgcgcttc ttgtgcttac
     3781 gtacatatat cttggcggag tgactgccgc acacacggca cttacggcaa tgtcagacct
     3841 tgtaccttcc tcgctcgctg ctcagggaat gaccggatgg acttccatgc catcccttgg
     3901 ttctcctatc tggtggaccc tgatctcgac actggtgctc ggtgtgggaa tcggggttct
     3961 tgcacagccc cagctggttg tccgtttcat gaccgcaaag gacaataaat ccctaaaccg
     4021 ggctgttgcc atgggtgctc cgtggctcct catgatgacc ggtgtcgcat tcactgtcgg
     4081 tgcactgacc aatgtatatt tctaccagca aacgggaaag atcgctattg atgcagcagg
     4141 aggcaacgtt gatgccatca tcccggctta catcaacctg gcaatgccgg acatctttgt
     4201 tattatattc atgctcaccc tcctgtctgc cgcaatgtcg acgttgagtg gcctttacca
     4261 tgcaatgggt actgcactta tctgcgacat ctggggaaga gggcgggcct gtgccctatc
     4321 catgaaagcg aaccaggccg gggtaataat aatgatggtt gccagtgttg cacttgcatt
     4381 cgtgatgccg acaaatatta ttgcgagggc aactgctatg ttcatggggc tttgtgctgc
     4441 tgcattcctt cctgcatttg ctgccggcat ttacgctaaa aatccatcaa caaaagccgc
     4501 actctggagc atgatatccg gttcaatctg ctggttcctg tggacggcat ttatacacac
     4561 cgcggaatca aaacccctcg gtctttgcca gtttatattt ggcaaacccg ctctgctgcc
     4621 actcccatgg acagtcattg atccgatcat tatcgccttg ccagtttcag ctgcaatcat
     4681 gatactgatg cagtggcagt ccggcagtaa gaataaatca atatctccct caatctccga
     4741 ataacccaca aaacatcgcg aaatgccgca cagatggaaa aattgcccat ctgtgctgga
     4801 caaacttttt ttgtatagag aaacgagaaa taatcatgga actcgcacaa aaaaaatgct
     4861 ctacctatag aatagggtcc ccgcctctga caagaaagga gatatttgag ctcatggggg
     4921 aattgccggg ctggtcgctt gacaatggtc atctgataag gatttttgag tttgatgaca
     4981 gcggccagtg cgtcgggttt tttaacgaag tggttgctct gtccacagaa gaggggcata
     5041 ttccggatat ctgcatgaaa gagtcaagat tcgtcgagat ttcatattac acctatcctg
     5101 ccgggggatt gactttaaac gactttatta tggcagctaa actgaatacc cgcagttaat
     5161 cttttttatt tcaggttaaa tttcttaacg attttttccc gcaatgcttc ttttggatac
     5221 gccccgataa tgcggtcgac cagctgacca tgcgaaaaaa gcattatgga gggtattgca
     5281 gtgatattga atttcaaggc aagggccctg ttctcttctg tattacattt accaaaggta
     5341 accttcccgg agaactcttt tgacaggtct tccagcacta ttccaaccct ccggcaaggt
     5401 gcgcaccatt ccgcccagaa atccagaact gcaaattgat gagtcttgat gaattcctga
     5461 aaatgcatct catccacctc agatatttct gcttttaccg tgtgtttcat cctgtctttc
     5521 agctcctgta gccgcttctc ccgtatccgg ctaatctcat catccataat tctctcttga
     5581 ataaaagagt acttaaatct cccgacccgg gcattgatac atgaatcaat tgagtaaagt
     5641 atatcagcgc acgccgacaa tagtataacc ctgattagag aagcgaggta gggtagtcag
     5701 gatatcccga cgggctcata acccgtagat cgatggttca aatccatccc tcgctactgt
     5761 ttcttttcat acatgtgctt aaaacaacgc tatacctgac aatcctgtac tatctgcacc
     5821 cattttggat gacgggggat ttacgggttt atgctttttt ttgcaacagt caccatgaac
     5881 gtccgattct taaaaacaac atccacatca gaaaatccgc actcatgcag ccactgcagc
     5941 tgcacagaca ttttttcgtt ccggtcaagg atgtcgcggc ggga
//
LOCUS       DASRLV010000024         6896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_980903,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000024 DASRLV010000000
VERSION     DASRLV010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980903"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VFC43_00805"
     CDS             <1..293
                     /locus_tag="VFC43_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HZK29763.1"
                     /translation="SGKPVNLLLEPLPKLLSEVLQQYHPVIIAFKLDSSQKNTAKKIL
                     KQGIAMVLMNSPENMGSPDGEYVLMTQEGELPLSGKKDEIAMAIWDAVMRGL"
     gene            484..1350
                     /locus_tag="VFC43_00810"
     CDS             484..1350
                     /locus_tag="VFC43_00810"
                     /EC_number="2.7.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate kinase"
                     /protein_id="HZK29764.1"
                     /translation="MKSPHVVAFCPGHISGYFKRIQGDTVATTGSIGAGMVISEGVTA
                     IVEPADHTSICIRRRDYNGKTSEDTRGSPLLEHVMENLTITASVVTESVLPIGAGFGL
                     SAAALLATLTALNRLYSLEMNAEEIAEVSHAAEVAHRTGLGDVAACQGGGRVLRYDPG
                     IHGRIERRFDMPEPLYAVSFGPIHTPTVLGSSEQMDRISDAFPRTIPKDHEDFFLLSR
                     HFAELSGLITEEVRDITRLCDAAGVASGMTMLGNGIFAYGKKARDILLPSGHVFELHV
                     AATGARILEERL"
     gene            1347..2078
                     /locus_tag="VFC43_00815"
     CDS             1347..2078
                     /locus_tag="VFC43_00815"
                     /EC_number="6.3.2.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphopantoate--beta-alanine ligase"
                     /protein_id="HZK29765.1"
                     /translation="MIPSDHPRYRSLLTRERLAECAKKGIVAWEGLTAHGRGEAFDYL
                     IGERTTESALFAERVAAALLLKARHPVISVNGNTAALAAPEISALQKACGARVEVNLF
                     HRTETRIKKIETLLLKSGVAISTGNAEQLLPLSHDRAWCRRDGIFSADVVLVPLEDGD
                     RCQALVDMGKTVIAIDLNPLSRTARSATMTIVDELTRALPHITQACYEISMDEKEKLI
                     NSVDNHYLLSEAIREMTVRLSFALD"
     gene            2065..3060
                     /locus_tag="VFC43_00820"
     CDS             2065..3060
                     /locus_tag="VFC43_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZK29766.1"
                     /translation="MLWIEKYRPEILTDILGQDPVIQRLSSFSVSGTIPHLILTGPHG
                     TGKSAAIECFAKALYNENWELNTSFFQTADLFSLGKAYLEQDDRYSHLYQKNQTLITN
                     FKYIIKWYASLRPLDAEFKLMVFEDAHALTKEAQQALRRIMERTSTTCRFVFTTTNQS
                     ALITPITSRCLPLLFSPVDQTVMLERLRTIRESENAGSRSCSDDDLELIVQVAQGDMR
                     RALLLLQVALEAGRCKDLFAIAQSETANVTASAIDALKNGEVRTAIRRLESLMIDYGL
                     TGSEVLLEIRRITQRDYNHPSLAIALADAEFRIRHGNNEFVQMGAFISGIQEVFT"
     gene            3057..3788
                     /locus_tag="VFC43_00825"
     CDS             3057..3788
                     /locus_tag="VFC43_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZK29767.1"
                     /translation="MSAVKQIKIQEHYDTIADVYDHHYDQRRGRSYHKHISTHIMNAL
                     PQGGKLLDIGCGTGLFVQRYIEAGGNAIGLDISRKMIEQARRRCRTCDYTIGIGEKIP
                     FYDNSFDAVSSLLVFSYVKDPASMMNEAYRVLKPGGSIAICTLGKKLLTRGIPAIYQI
                     GEKMNVKHVVMKDFGEHYYDEKEMDELFTHAGFTDINIKWCSFAHIDMIDPLFLLAQK
                     IEPFVEKRIPQLAYNIFVSGKKPGK"
     gene            complement(3791..5122)
                     /locus_tag="VFC43_00830"
                     /pseudo
     CDS             complement(3791..5122)
                     /locus_tag="VFC43_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106368.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
     gene            complement(5184..6164)
                     /locus_tag="VFC43_00835"
     CDS             complement(5184..6164)
                     /locus_tag="VFC43_00835"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZK29768.1"
                     /translation="MDIVSMIESPKVSESTQSPNKDQDYYLQGLSLGREGRHEEALVA
                     FSEALSSDPNRPLAWIGKGFALGKLGRYEEEIECCEKAIALDPNCVDAWIFMGFAFGK
                     LDRFEDKVKCCEQALKINPESAVAWNNKGVALGMLGQFEEEIRCCDRALKLRPRYLSA
                     WVNRGYAFGKLKWYEEEIICYDRALKIYPYYFSALVNKGIALIHLKRYREAVQVLDRA
                     LSIEPQNAIALYRKGLSLSLHGNHEEAIPCLEHALRINPSIADAWVVMSNSCFMAGRL
                     EESARAFDKAYYLDVKDARTGMVKGLFLLKTGKFDEAIRSFSNVWGILLR"
     gene            6357..6881
                     /locus_tag="VFC43_00840"
     CDS             6357..6881
                     /locus_tag="VFC43_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29769.1"
                     /translation="MYAYSRQDEASSYPIGVILLVAITCLLALLILLMIQIPPITLNM
                     EREIPVIFAITSLESVDELTGYMNYDSRILIRHTGIEDYQNKNLKAKFFKNSQPVTCT
                     IATLNGHDFISTYHTGIQWMGGSGCSGVTWSPGEQICIDFSDGTFHPGDNIQMDIIDK
                     VTNSTISRHLYKVV"
BASE COUNT         1854 a         1671 c         1564 g         1807 t
ORIGIN      
        1 cgagtggcaa accggtaaat cttttactcg aaccgcttcc aaaactcctt tctgaagtgc
       61 ttcagcaata ccatcctgtc atcattgcat tcaagctcga cagttctcag aaaaatacgg
      121 caaaaaagat actaaaacaa ggaattgcga tggtgctgat gaactctccg gaaaatatgg
      181 ggagtcccga tggcgagtat gtactcatga cgcaagaagg ggaattaccc ttgagcggaa
      241 agaaagatga gattgctatg gcaatctggg atgctgttat gcggggactt taataaaaat
      301 ttccccattc tgatcctttt aatggtaata actgcctctt ttttccctaa aggattaatc
      361 ctgggtttta taagatacct ctcataccag atcctagtca tcggtaagca gggaaaaatc
      421 aataccgggt ttctttaatg agggaaaacg ccctatcttc ttaataaaaa tgtgcaccag
      481 cccatgaagt cacctcacgt cgtcgcattc tgtccgggtc atatttcggg ctatttcaaa
      541 agaatacagg gggataccgt cgcaactacc ggcagtattg gtgccgggat ggttatcagt
      601 gagggggtta ctgcgattgt cgaacctgca gatcacacct cgatttgtat caggcgcaga
      661 gattacaacg gaaaaacaag tgaagatacc cgcggatcac cattactcga gcatgtcatg
      721 gaaaatctta ccataactgc atctgtagtc actgaaagtg tgctgccgat tggtgccggg
      781 ttcggactct ctgcggctgc actccttgct actcttactg ccctgaaccg cctgtatagt
      841 ctggaaatga atgcagagga aattgctgag gtttcacacg ctgctgaggt tgctcaccgg
      901 accgggctgg gagacgttgc agcatgtcag ggaggtggca gggtcttacg gtatgatccg
      961 gggatccatg ggaggataga aaggagattt gacatgcctg aaccattata tgcagtaagt
     1021 tttggcccga ttcatactcc aacagttctt ggatcgtcag aacagatgga tcgcatttca
     1081 gacgcttttc ccagaacaat accaaaggac cacgaggatt tttttttact ttcgcgccat
     1141 tttgccgagt tgagtggtct tattacagag gaggtccggg acataacccg gctgtgtgat
     1201 gctgccgggg tagcctcggg tatgacgatg ctggggaacg ggatttttgc gtatggtaaa
     1261 aaggcacgtg atatattatt accttcgggt catgtatttg aactccatgt agcggcaacc
     1321 ggcgcacgca ttctggagga gcggctatga taccttcaga tcatccccgc tatcgttcgc
     1381 tgctgacccg ggaacgactc gcagaatgtg caaaaaaggg gattgtagca tgggaggggc
     1441 tgacagcgca tggacgcggt gaagcgtttg attatcttat tggagagaga actacagaga
     1501 gtgccctttt tgctgagagg gttgccgctg ctctgcttct gaaagcccgg cacccggtta
     1561 tttcggtaaa cggaaacact gcggcgctcg ctgcacctga aatttctgcg cttcagaaag
     1621 cgtgcggggc acgtgtagaa gtgaaccttt ttcaccggac agaaacgcgt attaaaaaga
     1681 tagagaccct tcttttaaaa tcgggagtag ccatatcaac aggcaatgca gagcagttac
     1741 ttccactttc ccatgaccgg gcctggtgcc gacgggatgg tatattttct gcagatgtgg
     1801 tccttgtgcc tcttgaagat ggagaccgtt gccaggctct tgtcgacatg ggtaaaacgg
     1861 taattgcaat cgatcttaac cccctctccc gcacagcacg gtctgcgacc atgacgattg
     1921 tagacgaact gacccgcgct ctcccacata tcactcaggc ttgttatgag atttccatgg
     1981 atgaaaaaga aaaactcatc aattcggttg acaatcatta tcttttgtca gaagcaatac
     2041 gtgaaatgac tgtgaggtta tcctttgctc tggattgaaa aatatcgccc ggaaatcctg
     2101 actgatatcc tgggacagga cccggtaatc caacgtctct cgtccttttc tgtatccggc
     2161 actattcctc accttattct taccgggccc catggaacgg gaaaaagtgc ggcaatcgaa
     2221 tgcttcgcaa aggcgctcta taacgaaaac tgggaattaa atacttcatt tttccagact
     2281 gcagacctct tttcactggg aaaagcgtac cttgaacaag acgaccggta ttcacatctc
     2341 taccagaaaa accagaccct gataacgaat ttcaagtata ttatcaaatg gtacgcttca
     2401 cttcgcccac tcgatgcgga attcaagttg atggtttttg aagatgccca tgcgcttaca
     2461 aaagaagctc agcaggctct gagaaggatc atggaacgta caagtactac gtgccggttt
     2521 gtttttacca caaccaacca gagtgcgctt atcactccca ttacatcccg gtgcctccct
     2581 ctcctgtttt cccccgttga ccagacagta atgctcgaac gtctgagaac catcagagaa
     2641 tcagaaaatg cggggagtcg ttcctgctct gacgatgatc ttgaacttat cgtccaggta
     2701 gcacaaggtg atatgcgccg ggcacttctg cttctccagg ttgcgctgga agccggacga
     2761 tgcaaggacc tttttgcaat tgcccaatca gaaacggcaa atgttaccgc atcagccatt
     2821 gatgctctca aaaatggtga agtgcgtacc gctatccgca ggcttgagtc gctgatgatt
     2881 gactacggac ttaccggcag tgaagtactc cttgaaatac ggagaatcac gcaacgtgac
     2941 tataatcacc cttcccttgc gattgcactt gctgatgctg agttccgcat tcgccacgga
     3001 aacaatgaat tcgtccagat gggagcattt atctccggga tccaggaggt ctttacatga
     3061 gtgcggtcaa acagataaaa attcaggagc attacgatac tattgccgat gtttacgatc
     3121 atcactatga tcagcgtcgg ggccgaagtt atcataagca tatcagcact catatcatga
     3181 atgctctgcc gcagggagga aaactccttg atatcggatg tggaaccgga ctgtttgtcc
     3241 agcggtatat tgaagcggga ggcaatgcga ttggcctcga cataagcaga aaaatgattg
     3301 aacaggcacg ccgcaggtgc agaacatgtg attatactat cgggattggg gaaaaaatcc
     3361 cgttttatga caattccttt gatgctgtct caagtctgct tgtgttcagt tatgtaaaag
     3421 atcccgcctc aatgatgaat gaggcatacc gcgttttaaa gccggggggt tcaatcgcca
     3481 tctgtactct gggaaagaaa ctgcttaccc gggggattcc tgcgatctac cagatcgggg
     3541 aaaaaatgaa cgtgaaacat gttgttatga aagatttcgg ggagcattat tacgatgaaa
     3601 aagagatgga tgagctcttc acccatgctg gttttaccga tatcaatatc aaatggtgtt
     3661 cttttgcgca tatcgatatg attgatccac tcttcctcct tgcccagaag atcgaaccat
     3721 tcgtggaaaa acgtattccc cagctggcct ataacatctt tgtaagcggg aaaaaacccg
     3781 gaaaataaaa ttattctggt ttcggatgca taagagagtt taaaactcgc ttttttttcc
     3841 ggatcgcagc gtccgaagca cgcaacactc cctctttcat ctcttcaaaa tcacgcactg
     3901 aggtgtcaac aacctttttg accggtgtat acgcagattc ccggaaacgg ggagtaaaat
     3961 cgttctcctt gccgtcaatc ataaggaccg attccggctt ccctttttcc acgtacccga
     4021 ttttatgcat cggcactccc gctgatttca cgacacgaca gatctcttct gatacgtctg
     4081 gtggggccac gataagcagt gcatcgagtg aaacaccaag ataatcgatc tgcagtcttt
     4141 cgagcatatc cctgacgtga ggttccacca gacttcctat agaattttca tcaataacaa
     4201 ttctgcaccc ggctgtctct gccatttcga atgcgtcacc cctcagcccg ccatttgtta
     4261 catcggtcat cgcatggacc cggaaaaaga catcactttt catcagtgtc tcacacgcga
     4321 taaggaaatt gagattgata gtatgctcaa caacttaagg aaatcctgaa tatattgctg
     4381 cggttgcaat cgtaccgccc ccggcccctt cggtcataag caggacatcc cccggttgtg
     4441 ttgattttct tgcggtcagg tgatcggcga caccgacagc cccgacacat ccggtaagac
     4501 ggttgccaag caccatatca ccaccgatcc gaagtgtgga accggtgaca agaggtaccc
     4561 ccattgcctc gcctacggtc gttatcccgg cggtataatc aaaaatcttt gccacatccc
     4621 catcatctgc aacatggata tctgaaaaaa gcattattgg ttttgcaccc ataacgtaaa
     4681 catccctgag tgtcgcgcgc gttacatgga atcccgcaag gaagggataa tcggataagc
     4741 gggaatgcat accatcaacc gtgcaaacga tatagttgcc cccggcacaa acagcaccag
     4801 catcatccat ttcatctacg ccgacggagg cggaggtttt tccgattatt tttgcaatct
     4861 gacgatgtgc aaagaaatct ccttttcctc ttgatcctac cccgaattcc cccattttca
     4921 cgccagccgg aacgaaatca aaaaaatcac ccgacagacc ccgggtgttt ttcacttctt
     4981 cgattacagc atgtgcaaac gcggatgcat aggagggttg aacctttttt atggcgagaa
     5041 tatgatccac cagatttttc tgtaaggtat cctcatccat gccttcggcg attttcctcc
     5101 gggtaaactc ctcaacatcc atagtcttct gttcgtgcga gagacataaa aaagcggggg
     5161 ctatgggcaa attttgaaaa atactatctt aaaaggatac cccagacatt ggaaaaactt
     5221 ctgatagcct catcaaactt tccggtcttt aaaagaaaga gaccttttac catacctgtc
     5281 cgtgcatcct tcacatcgag gtaatatgct ttatcaaatg cgcgtgcaga ttcttccagt
     5341 ctgccggcca taaaacagga gttgctcata acgacccatg catcagctat cgatggattg
     5401 attctcaggg catgttccag acacggtatt gcctcttcat gattcccatg cagacttagt
     5461 gatagtccct ttcggtaaag ggcaattgca ttttgtggtt caatagaaag ggccctgtcg
     5521 agaacctgaa ctgcttcccg atatcgtttc agatgaatta aagctattcc cttattgacc
     5581 agtgcggaaa aataataggg ataaatttta agggctctgt cataacagat tatctcttct
     5641 tcataccatt tcagtttgcc aaacgcgtat cccctgttca cccatgcgga cagatagcgt
     5701 ggcctgagtt tcagtgcccg gtcgcagcaa cgaatctctt cttcaaactg acccagcatt
     5761 cccagagcca cacccttatt attccatgca accgcacttt caggatttat tttcagtgcc
     5821 tgttcacaac attttacttt atcctcaaac ctgtcgagct ttccgaaagc aaagcccatg
     5881 aaaatccagg catcgacaca gtttggatcc agtgcaattg ccttttcaca gcactcgatc
     5941 tcttcttcat accgtccaag tttcccgaga gcaaaacctt tgccgatcca tgcaagcggg
     6001 cggtttggat cagaggagag cgcctcggaa aatgcaacta atgcctcctc gtgccttcct
     6061 tcccggccca gagagaggcc ctgaagataa taatcctggt ccttgttggg agactgcgtg
     6121 ctttcagaaa ctttgggtga ttctatcatg gaaacgatat ccatacaatg gggtgtgagt
     6181 acttaccaaa ttcttagtgc cggtgatata tatgttaacg gattaaacta ggggcaaatc
     6241 ggtttatacg ctaaaaacag agttgattta ccccagacag atagaaatga tccgatgtta
     6301 ccttaaagaa atcaatttca gcaacgttat attgtccccc aatttatcta gcatcattgt
     6361 acgcttacag caggcaggat gaagcttcgt cctatccgat tggagttatt ctcctggtgg
     6421 caatcacatg ccttctcgcc ctcctcattc ttctcatgat ccagattcct ccgatcacat
     6481 tgaatatgga aagggaaatt ccggtgatct tcgcgattac atcactggag agtgtagacg
     6541 agctgacggg atatatgaat tatgacagcc gtatactcat ccggcatacg ggtatcgaag
     6601 actaccagaa taaaaatctt aaggcaaaat tttttaaaaa ttcacaaccg gtaacctgca
     6661 caattgcaac gctgaatgga cacgatttca tcagtaccta tcacaccggt atccagtgga
     6721 tgggagggtc aggatgttcc ggtgtaacct ggagtcccgg tgaacagata tgtatcgatt
     6781 tttccgatgg gacttttcat cccggtgaca atatacagat ggatatcatc gataaagtga
     6841 ccaacagcac catatcgcgg catctctata aagttgttta aacaggtatg tagttt
//
LOCUS       DASRLV010000025        12162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_493806,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000025 DASRLV010000000
VERSION     DASRLV010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 12162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12162
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493806"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="VFC43_00845"
     CDS             complement(<1..1078)
                     /locus_tag="VFC43_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF460 domain-containing protein"
                     /protein_id="HZK29770.1"
                     /translation="MRDRLRVFGIDVIKGSVRSRSRRPMYALVRMEGQTIESEAEVSM
                     FRLFRALNEEQPDILAVDSLQEIAADQHELFFFLQKLPPQTRLVQVTGGERKETLGKV
                     AARYNISFNRFDPWAEARTTAQVASLGAGAEVIAFENESDVVVSRHRSLGKGGWSQNR
                     YVRKIHGAVQSKGREIEMALVASGLRYEKKETRAFGGCSRVAFRVFATREKVPVSTYH
                     GADVQVRISGKRLERIQFRPLSGKPRYLIAGIDPGTTTAVAALDLDGNLLHLASSRQM
                     NMGDVVESLFRVGKPLIIASDVQEMPYSVEKIRRAFSAVAYTPKQDVSVETKIELTSS
                     FAYGNDHERDALSAALDAYRQYRHK"
     gene            complement(1063..1920)
                     /locus_tag="VFC43_00850"
     CDS             complement(1063..1920)
                     /locus_tag="VFC43_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HZK29771.1"
                     /translation="MVIAAERIRTLNKYEKSILNALERGMKHFAWVPLDQIKLVTKLS
                     ESEVNYRLARLIDWGMVRFNPVPYDGYSLVFGGYDALALMTLTRNGTISALGTKIGEG
                     KESVVYEALGLGPVAIKLHRVGQRSFSSPRLNRDYMPEEGHCPWLIASRRSAEREYQA
                     LTALHPKVSVPLPIGQNRHAVVMALINGPNLNHCVLEEPEEVLDDILLNAHHAYHEGI
                     IHADLSEYNILMDEGKCVLIDWPQWIGTTHPNAETILERDIDNVLTFFKRKYNMQYDR
                     GDAIRCVID"
     gene            2033..2929
                     /locus_tag="VFC43_00855"
     CDS             2033..2929
                     /locus_tag="VFC43_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK29772.1"
                     /translation="MQCSKCGRTAVFFQTYSGQHLCLHHFTADVEAKAKHEIRRNHSL
                     RSGDHIAVGLSGDACSSALLYFLKKLTSNRRDIRISAISIDEGIAGYRFLGDSERITE
                     LLQTECIAQSFQENVGRTLDDIAETKGKTRSCTYCRVIRNFLLNRIARENGVTKIACG
                     STLDDAAGSVLNHILQGIPEIVVGSDVSFRGKIPRIRPFIAVPKEEIALYADLHVKEY
                     NRSHCPYTNNHFDEDVDAMLNEFTLHHPATKYALLSLKKNLARACVSLPDFSSSCERC
                     GEPVIGICQNCRIIDEVTSHGA"
     gene            2919..4625
                     /locus_tag="VFC43_00860"
     CDS             2919..4625
                     /locus_tag="VFC43_00860"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HZK29773.1"
                     /translation="MALRRGRVWRAIRNSPGIQISIYFLAFAVLIGVNTYIFHSYYPL
                     LENKSLSWTESLLFVVESMTTVGYGWLLPFSHDITRLLAIQIMLSGVIMIFVVIPLLL
                     APFLTTLLAPSPPRRTPHRLSGHTVVMGYDKLTSSVIDSLTISDHDIVIIEQDKAKAL
                     EIATHYRKRAYVIWGDYTDPGTWSAAHITGAHYIVICKDERQTASIVLGIRGLVKGKI
                     ISIVDKITFDRYLRYAGADYVLSPKHETGRILARHAVLNPSGYAEPDLPGLDRININI
                     RQASEHELRLINIPLVFGCRAAFKSLHELRLFERFGVIVPFLWKAGKFISFPTDDIIV
                     DNTTSLFLFGRAGSIVATLEEEFNTEGCADAFAVIAGFGDVGVGAYQELLASGVSCIV
                     VDSKQQNMEQVVGDAEDENVLKQARIEEARFCIIAVNDDNINIFTTLMARNLNPVIRI
                     LARANEPSSVEKLYRAGADYVALLPMIGGQAIARIILADTVTILLELPNNDLVILKRV
                     RSTYPKSLNWLTRKTGARIIGIELTDRSIVAPDPEEILSEGDAVIAVGNTEQLKKFIR
                     LL"
     gene            4641..5396
                     /locus_tag="VFC43_00865"
     CDS             4641..5396
                     /locus_tag="VFC43_00865"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HZK29774.1"
                     /translation="MSTDLGCRMGQVEQMIRYTYWNSGCTGIVIGLSGGVDSAVAAAF
                     CCRAIGSDKVLGLSLPSSVSDPADINDAAALCARTGITHRIISIDPMLDGFKTMPEFK
                     ESRYLVGNLMARIRMATLYYYANRDQKIVCGTSNRSEYMLGYCTKFGDNAADLQPLLH
                     LYKTEVYEYAHELGIPEAIIKKAPSAGLWEGQSDEEEIGLSYSEIDSSLKGLEQQDWE
                     AVTPLQEKVLALVKKSEHKRLIAPNLLAVQKIR"
     gene            complement(5365..6072)
                     /locus_tag="VFC43_00870"
     CDS             complement(5365..6072)
                     /locus_tag="VFC43_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HZK29775.1"
                     /translation="MIGWAGELPQPAIRTIEDMRPVLADPSCRSSGPLYFMYRDLARS
                     DADWNWLRHHNLRYDLTVIPSSEICGERVKTKGHYHPKNPAGIGYPEIYEVIEGKVHY
                     LLQSRNLDDIVMISAQAGDLVIIPPEYGHVSINPSTDSALVMANIVSTAFESEYGEFE
                     KYRGAAYYEMSDGKMQKNIRYPSLPPVRYIAATCRKEIHPLCRGPLYNLIGNEDALSF
                     LNYPENFLTVFSVLLKD"
     gene            complement(6072..7361)
                     /locus_tag="VFC43_00875"
     CDS             complement(6072..7361)
                     /locus_tag="VFC43_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HZK29776.1"
                     /translation="MKSFLIMSISLVLVLWCTIPVMGVTTYLGGSPQMSAAISGTNEF
                     TPGQDAIIQVIVWNSGVNNLKFVTKGTIERDDIATTAKMVTVGLFVGDAPIVVKNDPQ
                     LIGDIKSQGTATVAIATKITADATEGEYQLPLNIRYTYLASSDQEAVDVLQYNYQPVN
                     ETIPITIKIKPQVKIEILDAVSENLNIGSEGYLNLTIKNIGLEDGKKASVKILRNGAS
                     PIIPTDSSVFIGDFPHNSTVTCRYKVVVSDEAEIQSYPVDVLVMYENREGDVVSSAAD
                     TIGIPVVGKISFIITSGAVRVLQGSQSVIQIVYKNTGTRPAYHAQARLSAVEPFKSSD
                     DTAYLGDIRPGENATAQYQVQVDDAAIVRNYTLDTEVRYRDALDNSQVSDPFRVAFEV
                     QPKPSTGGLIQVLGIIGIIALIGIGAGYYLLVMRKKK"
     gene            7427..8206
                     /locus_tag="VFC43_00880"
     CDS             7427..8206
                     /locus_tag="VFC43_00880"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZK29777.1"
                     /translation="MKLPSENPVRIIDCLKSLGLTKYEALVYIALLKLVSATASEIHE
                     ISDVPRASVYPVIDQLLVKGLVSVSQSAPKRFAAISPEDAISRLMDKIKKDAYFARDS
                     LSLLYKERMNRGLGTEELIWNIYGIGNIKNRFSDLISGAKQEIRIIAHPEILTPDIQQ
                     KLATMANQISIKIITPHWAGEMPGKLKVYSIRQPEIPKELDMAKDMMAGGVCIIDSRS
                     VMVILGVGEDDGVALFSESEGFVRFFVRYYNLIFNWAKKLK"
     gene            complement(8225..8989)
                     /locus_tag="VFC43_00885"
     CDS             complement(8225..8989)
                     /locus_tag="VFC43_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF128 domain-containing protein"
                     /protein_id="HZK29778.1"
                     /translation="MSEITIMTVPLKFINHSIEDYAMKVTFDPATGEGKVVYNLSVIK
                     NEDLDFAISTLKDAYKTGVTVSGRVRFLHSGDKLPGYVVPKGYTGICTVCSITFDGIL
                     IRRGIPITPVGGGVVEIEKRTPIRFTHIILYEHTTIDPLQVLISQKTTSITSVMRTGS
                     GSILANVREFHMEAEHQVGLVLDELSASTLSGILEVGMPNLPLLGVQVSPQFVAIAAV
                     GGTNPMAAIREGGRWVQTQAMKGLMEISQLEEIRDY"
     gene            9032..10000
                     /locus_tag="VFC43_00890"
     CDS             9032..10000
                     /locus_tag="VFC43_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29779.1"
                     /translation="MMEHDMREKAKHAMRLILEAARFEVEEVDDPLDLSAVRDETCLL
                     VLCSNESDLISQFDKTNYSIMIDDHEMTCKKLLFTIDKDIVTENCIHWGVDEFVRYTG
                     EAVLADILDRELALDLTPSKGRKTTVAAPAGGQEETRKVEPSGITIPHYPIKIAEQAA
                     IRTAGVQGTAKLRFIPYYLFHYSSSGEQVYKDRRIPFDADGWGAINAINGIKIDLDDK
                     LVEDTEIPGGAEVSDPHIRKDEATERIVNELIDRLTQKVRIKQEKGDAIFYEEKILKP
                     DRKNITVDTRQVYIPIWQIRGKKIVEINAFTGELLSEPMDEGVEIF"
     gene            10092..11423
                     /locus_tag="VFC43_00895"
     CDS             10092..11423
                     /locus_tag="VFC43_00895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZK29780.1"
                     /translation="MIVVLGGGPAGRIASIRLASAGKEVHLIENGRIGGQCLHFGCMP
                     VCALNDTARHIHSARTFHNLGITDTIPKINFSKIISEMHAIQEKIASILDAETRSAGV
                     NIIYGKTGRLDSGNVFVGEEQLDAEAVIVATGSRPGIPALDGIDLNGVYTPHTLFQMK
                     ELPEKIVIIGGNVMAAEFAYIFSMFGSKVTILSRSAFLKNADSHLRALAIRQLHEVEI
                     REGTKINSLTGKERIESVHFQVHDRYESVDADAALIATGLIPRSELLNGIKKGPLGEV
                     IVDEHMRTSIPDVYACGDVVGPPYLTPIARHQGIVAADNILGIPRKMDYRFIPQSINL
                     SQELAFCITEKEGLASLAIPGPAGPGTFWEVPASDTGLAKILFDPESGTIKGMCAAGP
                     GGGLIAGYMSFLMKHHFSIHDFEEFIEVHPSTDGVYGIAKYASELMKKRNK"
     gene            11561..>12162
                     /locus_tag="VFC43_00900"
     CDS             11561..>12162
                     /locus_tag="VFC43_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HZK29781.1"
                     /translation="MTSPTENTAFDQTRLDKIRALQDKGITIFPSSFDRQHTVLDIKM
                     KYTDITHDKSEERVSTAGRIYIVRNHGKTVFADLGDETGKIQLYIRKNDLGDEQFDLF
                     NQFIERGDIIGVTGHVFRTKLGEITIWVDSFTLLCKAVCSLPEKFHGLTDIEKRYRQR
                     YVDLIVNEESRKIFRSRSRIVSLIRRYLDDHGFLEFETPIL"
BASE COUNT         3112 a         3002 c         2923 g         3125 t
ORIGIN      
        1 atttatgccg gtactggcga tatgcatcaa gagcggcaga caatgcatcc cgctcatgat
       61 catttccgta tgcaaaggat gacgtaagct cgatcttggt ctcaacactc acgtcctgtt
      121 ttggggtgta tgctaccgca gaaaaggccc tccttatctt ttccaccgag tagggcattt
      181 cctgtacatc cgatgcgata atcaggggtt ttcccacgcg gaacagcgac tcaacaacat
      241 ctcccatgtt catctgccgg gaactcgcca gatggagcag gttaccatcg agatctagtg
      301 cagcaacagc tgttgtagtc cccggatcga taccggcaat caggtatctc ggcttgcccg
      361 agagtggacg gaactgaatt ctctcgagac gtttgccgct gatccgtacc tgaacatcgg
      421 caccgtgata tgttgatacc ggaacctttt cccgcgttgc aaaaacccgg aatgccaccc
      481 ggctgcagcc cccaaatgcc cgggtctctt ttttttcata ccggagaccg gatgcaacaa
      541 gagccatttc aatttcccgt ccctttgatt gcaccgctcc atgaattttc cgaacatacc
      601 ggttctggct ccacccgccc tttcctaatg accggtgacg gctcaccaca acatcacttt
      661 cgttctcaaa tgcaatgact tctgcacctg ctccaaggga cgcaacctgc gctgttgtcc
      721 tggcttcagc ccagggatcg aaccggttaa agctgatgtt ataccgtgca gcaaccttgc
      781 caagtgtctc cttgcgttcc cccccggtca cctggacaag ccgggtctgg gggggcagct
      841 tctgcaaaaa gaaaaaaagt tcatgctgat cggcagcgat ctcctgcagg ctgtcgaccg
      901 caaggatatc cggttgttct tcattaagcg cccggaaaag acgaaacatt gagacctcgg
      961 cctcgctctc gatggtctgt ccttccatcc gcacaagggc atacatggga cgccgggacc
     1021 gggaccttac tgaccctttg atgacatcaa taccaaaaac ccttaatcga tcacgcatcg
     1081 aatcgcatcc ccgcgatcat actgcatgtt gtattttcgt ttaaaaaaag tgaggacatt
     1141 atcgatatcg cgctcaagga tcgtctccgc attcgggtgg gtagtcccta tccactgtgg
     1201 ccagtcgata agtacacatt tcccttcatc catgagaata ttatattctg acaggtcggc
     1261 gtggattatc ccttcgtgat atgcatgatg tgcattcaat aggatatcat ccagcacttc
     1321 ttctggctct tcaaggacgc aatggttgag gttcggacca ttgatcaatg ccatcaccac
     1381 cgcatggcgg ttctgtccta taggcagggg aacactgacc tttggatgaa gtgctgtaag
     1441 tgcctggtat tcccgttccg ctgatctccg tgacgcgatc agccagggac agtgaccttc
     1501 ctcgggcata taatcccggt tcaggcgggg ggatgagaat gatcgctggc caacccggtg
     1561 caactttatc gcaaccgggc caagtcccag agcttcgtat acaaccgatt cctttccttc
     1621 acctatcttc gttccgagtg cagatatcgt accgttgcgg gtaagggtca tgagagcaag
     1681 cgcatcgtaa ccgccaaaaa caagtgaata tccatcgtag ggtacgggat tgaaacggac
     1741 cattccccag tctatcaggc gggcgaggcg gtaattgact tcggattcgg acagtttggt
     1801 caccagcttg atctggtcca gcggtaccca tgcaaagtgt ttcatcccac gttcaagtgc
     1861 gttgaggatg gatttttcat atttgttgag ggtgcgtata cgctctgcgg cgatgaccat
     1921 aatcgttaga gtggttatgt gtcagactat aaaatcacat ctctggtgct gatttaacag
     1981 ctaacacaac cttaaacatc tttaattatc cagatcacaa aggaatttgc aaatgcagtg
     2041 cagtaagtgc gggagaaccg cagtattttt ccagacatat tccggacaac acctctgctt
     2101 acaccatttt actgctgatg ttgaagccaa agcaaaacac gaaattcgca ggaaccactc
     2161 tttgagatcc ggtgatcata ttgcggtcgg gttgtctggt gatgcctgca gcagcgccct
     2221 cctctatttt ttaaaaaaac tcacctcgaa ccgccgggac atacgtattt ctgccatctc
     2281 gattgatgaa ggcattgccg gataccgttt cttgggagat tcagagcgta ttaccgaact
     2341 gctgcagact gaatgtatcg cccagtcatt tcaggaaaac gttggaagga ccctggatga
     2401 tatcgcagaa acaaaaggta aaacccgttc ctgtacgtat tgccgggtga tcaggaattt
     2461 cctgctgaac cggattgccc gggaaaacgg ggtaacaaaa attgcctgcg gctctacgct
     2521 ggacgatgcg gctggttcgg ttctgaacca tatactccag gggattcctg agattgttgt
     2581 tggttcagat gtatcattca gaggaaaaat tccccggatc cgaccgttca tcgctgttcc
     2641 gaaagaagag atcgcgctct atgcagatct tcatgtcaaa gagtataatc gatcccactg
     2701 tccttatact aataatcact ttgatgagga tgtggatgcg atgcttaacg agtttaccct
     2761 gcatcacccg gcgacaaaat atgcactcct gagcctgaaa aaaaatctgg cacgtgcctg
     2821 tgtttccctg ccggattttt cttcgtcctg tgagcgatgt ggtgaacctg ttatcggcat
     2881 ttgccagaac tgccggataa tcgatgaggt tacttcccat ggcgcttagg cggggaagag
     2941 tctggagggc aatccgtaat tctcccggga tacaaatctc gatctatttt cttgcgtttg
     3001 ccgtgctgat aggagtcaat acctatatat ttcattccta ctacccattg ctggaaaata
     3061 aatccctttc gtggactgaa tcacttttat ttgtcgttga gtccatgaca acggttggat
     3121 atggctggtt attgccattt tcccacgata ttacacggct gctggcgata cagatcatgc
     3181 tgtccggcgt tatcatgatc ttcgttgtta tcccgcttct ccttgccccg ttcctcacca
     3241 ctctgcttgc tccctcaccc ccacgaagaa ccccgcatcg cctgtcagga catacggttg
     3301 tcatggggta tgacaagctc acgagttctg tgattgacag cctcaccatt tcagaccacg
     3361 atattgtgat catcgaacag gacaaggcaa aagcccttga aattgccact cattaccgca
     3421 aacgtgctta cgtgatctgg ggggattaca ctgatcccgg cacatggtca gcagcacata
     3481 tcacaggtgc acattatatt gttatctgca aggatgagcg gcagaccgcc agcattgttc
     3541 ttggtatacg cggactggta aaaggcaaga tcatttcgat cgtggataaa atcactttcg
     3601 accggtatct ccggtacgca ggtgctgatt acgtcctctc tcccaaacat gaaaccggaa
     3661 gaattctcgc acgccatgca gttcttaatc caagcgggta tgcagaaccg gacttacctg
     3721 gtcttgacag gattaatatc aatatccggc aggcatcgga acacgaactc cggttgatca
     3781 acatacccct tgttttcggg tgcagggctg cgtttaaatc attgcacgag cttcgtcttt
     3841 ttgaaagatt cggtgtcatt gtcccatttc tctggaaagc cgggaaattc atttcgttcc
     3901 ccactgatga cattatagtc gacaacacga cgtcactgtt cctttttggc agggctggat
     3961 ctatagtcgc taccctggaa gaagagttca atacagaggg atgtgcagat gcctttgctg
     4021 tgattgcagg gttcggggat gtcggtgttg gtgcatacca ggaactcctt gcttcgggtg
     4081 tatcctgcat tgtagtagat tcaaaacaac agaacatgga acaggtcgtc ggggatgctg
     4141 aagatgaaaa cgtcctcaaa caggcccgta tcgaagaggc acggttttgc atcattgcgg
     4201 tgaatgacga caatataaat atcttcacta ccctcatggc ccggaattta aacccggtaa
     4261 tccgtatcct tgcgcgggcg aacgaacctt cttcagttga gaaactgtac cgtgcgggtg
     4321 ctgattatgt tgccctcctt ccgatgatcg ggggtcaggc aatcgcgagg atcatccttg
     4381 cggataccgt cacgattctt cttgaacttc ccaacaacga tcttgtgatc cttaaacggg
     4441 tccggtcaac ctacccgaaa tccctgaact ggctgaccag aaaaaccggg gcacggatca
     4501 tcggcatcga actgactgac cgttctatag ttgctcccga tcccgaagag atcctttctg
     4561 aaggagatgc ggtaatcgcg gtaggaaaca ccgaacaatt gaaaaagttc atccgtttac
     4621 tataaacgga gtacatatca atgtcaacag atctcgggtg caggatgggg caggtagaac
     4681 agatgatccg ttacacctat tggaacagcg gttgtacggg aatcgttatc ggtctgagcg
     4741 ggggcgtcga ttctgccgtg gctgccgcgt tctgttgcag ggcaatcggg agtgataaag
     4801 tgctgggact ctctctgcct tcatcggtca gtgacccggc agatatcaac gatgccgccg
     4861 cactctgtgc ccggacggga attacacaca ggataataag catagatccg atgcttgatg
     4921 ggttcaaaac catgccggaa tttaaagaat cacggtatct tgtcggcaac ctgatggcac
     4981 ggatccgtat ggcgactctg tattattatg caaaccggga tcaaaaaatt gtctgtggta
     5041 cgtcgaacag gagtgaatat atgctgggtt actgcacaaa gttcggtgat aatgctgctg
     5101 acctccagcc tttactgcat ctgtataaaa ctgaagtgta tgagtatgct cacgaactgg
     5161 gaattccgga ggctattatc aaaaaggctc cctccgccgg tctatgggaa gggcaaagcg
     5221 acgaagagga gatcggttta tcctacagcg aaatcgatag ttctcttaaa ggccttgagc
     5281 agcaggactg ggaggcagta actcctcttc aggaaaaagt acttgctctc gtgaagaaaa
     5341 gcgagcacaa aaggctgatt gcgcctaatc ttttagcagt acagaaaata cggtaaggaa
     5401 gttttccgga taattaagga acgagagcgc atcttcgttt cctatgagat tatacaacgg
     5461 gcctctgcac agagggtgta tctccttcct gcaggtagcc gcgatatacc ggacaggcgg
     5521 taaagacgga taccggatat tcttttgcat cttcccgtca ctcatttcgt aatatgctgc
     5581 accacgatat ttttcaaact cgccatactc gctctcgaat gccgttgata caatattcgc
     5641 cataacgagc gctgaatccg tagagggatt gatggagaca tgcccatatt ccggcgggat
     5701 gatcacaagg tcacctgcct gtgcagagat catgactata tcatcgaggt tccgggactg
     5761 gagaaggtag tgcaccttgc cttcaataac ttcataaatc tcaggatacc cgattcctgc
     5821 cgggtttttc gggtgataat gccccttggt cttcacccgt tcgccacaga tttcgctgga
     5881 gggtatgacg gtcagatcat accgcaggtt atgatggcgc aaccagttcc agtcagcatc
     5941 tgaccttgcc aggtcccggt acataaaata gagggggccg ctgctcctgc acgaggggtc
     6001 ggcaaggaca gggcgcatat cctcaatcgt ccgaattgcc ggttgaggaa gctcccctgc
     6061 ccatccgatc atcacttctt ctttcgcatt acaagtaaat aatacccggc gccaattccg
     6121 atgagggcga taatccctat aattcccagt acctgtatca atccgcctgt tgatggttta
     6181 ggttgaacct cgaacgctac cctgaaagga tctgatacct gactgttgtc cagtgcgtcg
     6241 cgatacctta cctcagtatc aagggtatag ttcctgacga tcgctgcatc atccacctga
     6301 acctgatact gtgcggttgc attttccccg ggtctgatat ctccaagata tgcagtatcg
     6361 tcgctgcttt taaacggctc tacggctgac aggcgcgctt gtgcatggta tgccggcctg
     6421 gttccggtgt ttttgtagac tatctggatc acactttgtg atccctgaag tacccgaact
     6481 gctccggagg ttataatgaa actgattttt cccacgacag gaataccaat agtgtccgca
     6541 gctgagctca cgacatctcc ctcgcggttc tcatacatga caaggacatc aaccggatat
     6601 gactgtattt ccgcttcatc cgagaccact accttgtatc tgcaggtgac cgtgctgttg
     6661 tgggggaaat caccgataaa aacgctgctg tcagtcggaa tgatcgggct tgctccgttc
     6721 cgcaggatct tcacggaagc ctttttgcca tcctccaacc cgatgttttt gatcgtaagg
     6781 ttcaggtatc cttcacttcc gatattcaga ttttccgaaa cagcatcaag aatttcaatc
     6841 tttacctggg gcttgatttt aattgttatc ggaatggtct cattcaccgg ttgataattg
     6901 tactggagaa cgtcaacggc ttcctggtca gaagaggcaa ggtaggtgta ccggatattc
     6961 aggggcaact ggtattcgcc ttccgtggca tcagcggtaa tttttgttgc aatggccacc
     7021 gtggctgttc cctggctttt tatgtcccct atcagttgcg ggtcgttttt cacaacaatt
     7081 ggggcgtcgc ccacaaaaag accgacagtt accatctttg ccgttgtcgc gatatcatcc
     7141 cgctcgatag ttcctttcgt tacgaatttg agattattaa ccccgctgtt ccaaacgata
     7201 acctgaatga tcgcatcctg tcccggggta aattcgttgg tacccgatat tgctgccgac
     7261 atctggggac tcccgccaag atatgtcgta acacccataa ccggtatagt gcaccagagc
     7321 accagcacca gagaaatact cattatcaga aaagatttca tgaaccatca cttgttgtta
     7381 ttgttataac aacatttatt atagcaggat ataaagtgat gttttaatga aattgccgtc
     7441 ggaaaatcca gtccggatca ttgactgcct gaaatcactc ggtctgacca aatatgaggc
     7501 actcgtgtat attgcgctcc tgaagctggt cagtgcaacg gcaagcgaga ttcatgagat
     7561 ttcggatgtt ccccgtgcct cggtgtatcc tgttattgat caacttctcg ttaaaggact
     7621 ggtatcggtc tctcagtctg ctcccaaaag gtttgctgct atttcacctg aagacgccat
     7681 ctcccgactg atggacaaaa ttaaaaagga cgcatacttt gcacgcgatt ccttatcact
     7741 gttatataag gaacggatga accggggtct gggaaccgag gaacttatct ggaatatcta
     7801 tggtatcgga aatatcaaaa accggttcag tgatctcatt tccggtgcaa aacaggagat
     7861 tagaattatt gcacatcccg agattctgac gcccgacatt caacagaaac ttgctacgat
     7921 ggccaatcaa atcagtatta aaattatcac tcctcactgg gctggagaga tgccggggaa
     7981 actaaaagtg tattccatca ggcaacccga aattccaaaa gaactcgaca tggcaaaaga
     8041 tatgatggcc gggggagtct gcatcattga cagtcgatcg gtaatggtga ttctgggtgt
     8101 gggagaagat gatggggtag cacttttttc agaatccgaa ggctttgtcc ggttctttgt
     8161 gcgttattat aacctgatct ttaactgggc taaaaaacta aaatagccca ccgcaaactg
     8221 gaacttagta atccctgatt tcttccaact gcgagatttc cataagtcct ttcattgcct
     8281 gagtctgcac ccagcgacca ccttcccgaa ttgctgccat cggatttgtc cctcccactg
     8341 ccgctattgc aacgaactgc gggctcacct gtacgccaag gagaggaaga ttcggcattc
     8401 ccacttcaag aataccagaa agggtgcttg cggagagttc atcaagtact aaccccacct
     8461 gatgttcagc ctccatatga aattccctta catttgcaag aattgatccg cttcccgtac
     8521 gcataacact ggtaatcgat gttgtcttct gggagatcag gacctggagt ggatcgattg
     8581 tcgtgtgttc gtacagaatt atgtgagtga accgaatggg ggtgcgtttt tctatctcga
     8641 caacaccgcc cccgacaggg gtgatgggaa tcccccgccg gataaggatt ccatcaaagg
     8701 taatgctgca tactgtacag atacccgtat atcctttcgg caccacatac ccgggcaatt
     8761 tatctccgga atgaaggaat ctgacccttc cgctgacggt gacaccggtc ttgtacgcat
     8821 ctttaagcgt cgagattgca aagtcgagat cttcgttttt aatcactgac aggttatata
     8881 caactttacc ttcacccgtg gccggatcaa aagtcacctt catcgcgtaa tcttcaatgc
     8941 tgtggttgat gaatttgagc ggaacggtca ttatagtaat ttctgacatc gcaatagaaa
     9001 aattgttcta taagaccatg gcaaattatg tatgatggaa cacgacatgc gggaaaaagc
     9061 caaacacgca atgcggctca ttcttgaggc agcccgcttt gaggtcgaag aagttgatga
     9121 ccctcttgac ctttctgcgg tacgtgacga gacctgcctc ctggtgctct gttcaaatga
     9181 gagcgacctg atcagccagt ttgataaaac aaattacagc atcatgatag acgatcatga
     9241 gatgacctgt aaaaagctcc tttttaccat tgacaaggat attgtgacgg aaaactgtat
     9301 tcattgggga gtcgacgaat ttgtacggta tacgggtgag gcggttctcg ccgatattct
     9361 tgatcgtgaa ctggcattgg atctcacacc ttcaaaaggc aggaagacaa cggttgctgc
     9421 tccagctggc ggacaggaag aaacccggaa ggttgaacca tccggcatca ccattcccca
     9481 ttaccccata aaaattgccg aacaggcagc gatccggacg gcaggcgtcc agggtaccgc
     9541 caaacttcgt tttataccct attatctttt tcactattcg agcagtggcg aacaggtcta
     9601 taaggatcgg cgcattcctt ttgatgccga tggctggggt gcaattaacg caatcaatgg
     9661 aatcaagatt gaccttgatg acaaattagt cgaggatacg gagattcccg gcggtgctga
     9721 agtatctgat ccccatatcc ggaaagatga ggcaaccgag agaatcgtaa acgaactgat
     9781 cgacaggctt acccagaaag tgcgaatcaa acaggaaaaa ggcgacgcaa tcttctacga
     9841 agagaagatc ctcaaacctg acaggaaaaa tatcaccgtt gatacccggc aggtctacat
     9901 ccctatctgg cagatcagag gtaaaaagat tgttgagatt aatgcattta ccggcgaact
     9961 gctctcggaa ccgatggatg aaggagtcga aatattctga gagtggcggg ctgcacggaa
    10021 taaatatgat ttcccctgct cctcttgtaa tgaacaatat acctttttca ctgcatcttt
    10081 gaggaaatcc catgattgtt gttcttgggg gtggcccggc aggaaggatc gcatcaatcc
    10141 gccttgcttc cgcgggaaaa gaagtccacc tcatagaaaa cgggagaatc gggggccagt
    10201 gcctgcattt tggatgcatg cccgtctgtg ccctgaacga tactgcccgg cacatacatt
    10261 cggctcgtac atttcataat ctgggtataa ctgacactat accgaaaatc aatttttcaa
    10321 aaattatttc agagatgcat gcaatccagg aaaagattgc ctccattctt gatgccgaaa
    10381 ccagatctgc cggggtgaat ataatctacg gtaaaaccgg caggctcgac tcagggaatg
    10441 tatttgtcgg agaggaacag ctggatgctg aagcggttat tgtggctacc gggtcccgtc
    10501 ccggtatccc cgctcttgat ggtatcgatc tgaacggagt ctatactccc cataccctct
    10561 tccagatgaa agaactcccg gaaaaaattg tcattatcgg gggaaatgtg atggccgccg
    10621 aatttgctta tatattttcc atgttcggca gcaaggttac cattctcagc aggagtgctt
    10681 ttttaaaaaa cgcagattca catctcaggg cccttgcaat caggcagctc catgaagtgg
    10741 aaatcaggga agggacgaag ataaactcac tgaccggaaa agaaaggata gagtctgtac
    10801 acttccaggt tcatgacagg tacgaatcgg ttgatgctga tgctgctctt atcgcgacgg
    10861 gccttatccc acgatcagaa ttgctgaacg ggataaaaaa aggacctctt ggagaagtca
    10921 ttgtcgatga acacatgagg accagtatac cggatgttta cgcgtgcggg gacgttgtcg
    10981 ggccccccta cctgacgccc atagcccgcc accagggtat tgtggcagcg gataatatcc
    11041 tgggtatccc ccggaaaatg gattaccgtt tcatccctca atctatcaac ctgtcacagg
    11101 aactggcatt ctgtatcacc gaaaaggagg ggctggcttc tcttgcaatt cccggacccg
    11161 caggacccgg tacattctgg gaggttcctg caagtgacac aggacttgcg aaaattctgt
    11221 ttgatccgga gagcggcacg atcaaaggca tgtgtgctgc cgggcctggc ggagggctga
    11281 tagccggata catgtcattt ctcatgaaac accatttttc aatccatgac tttgaagagt
    11341 tcattgaagt acatccttcc acagatggag tctatgggat tgccaaatat gcatcagaac
    11401 tcatgaaaaa acgaaataaa taacaattcc ctcaattttt tatgtacatc agcaatgcct
    11461 gaagcgatag tgatctgtat gtcactttgc tgaaggtatt attaccgttc aaatcgcatt
    11521 attacgatac acaccatccc cgagcgaggg tgctttcaat atgaccagtc ccaccgaaaa
    11581 tactgcattt gaccagacac ggctcgataa aatccgtgcg cttcaggata aaggcattac
    11641 catatttcca tcgtcgtttg accggcagca cacggttctc gatataaaaa tgaagtacac
    11701 tgatattacg catgataaga gtgaggagcg ggtatctaca gccggaagga tctatatagt
    11761 ccggaaccac gggaagaccg tgtttgctga cctcggtgac gagactggaa agatccagct
    11821 gtatattcgt aaaaacgatc ttggggatga gcagttcgat ctcttcaatc aatttatcga
    11881 gaggggagac atcatcggag taactggtca tgtgttccgg acgaagctgg gtgagatcac
    11941 catctgggta gacagcttca ccctcctttg caaggcagtt tgttcccttc cggagaaatt
    12001 ccacggcctg accgatatcg aaaagcgcta tcgccagcgc tacgttgatc tgattgtgaa
    12061 tgaggagagc aggaagatat tccggagccg gagccggatc gtctccctca tccgcaggta
    12121 tctcgatgac cacggctttc tcgagttcga aacccctatc ct
//
LOCUS       DASRLV010000026        19934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_331824,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000026 DASRLV010000000
VERSION     DASRLV010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 19934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19934
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331824"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VFC43_00905"
     CDS             complement(<1..614)
                     /locus_tag="VFC43_00905"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK29782.1"
                     /translation="MKTRISLFFGMFAVMALSNAIVPVLPAYTDSSTAQAAIYSAYFL
                     GAFVSTLPAGILSDRYGRVPIIRAGLAITVASGFLLSVLVLPVPVIIARIIEGIGAGF
                     FVAAAMSYINSLPDHERMSGYLMASLNAGLVIGLVCAGWLAARLPNPASGILVFSALV
                     IIPVFASFFIRETGSVTREENFATILYLVQQFRWLWYSAVILIG"
     gene            complement(626..2545)
                     /locus_tag="VFC43_00910"
     CDS             complement(626..2545)
                     /locus_tag="VFC43_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-CASP ribonuclease aCPSF1"
                     /protein_id="HZK29783.1"
                     /translation="MLIEERLKELKDKINEKVPHGITVTQVEFEGPELVIYTDDPKRF
                     ADEADLIKVLARDLRKRIVVRPTILEDPEKAYNEIKSVVPDTAGITDIFFDADTGEVL
                     IEAEKPGVVIGKNGATLREITKHIGWTPKVVRTPPIESSTVKQVRQYLRSVNEERKSF
                     LRTIGRRIHRDIPGKDDTGKDTIKKDQWARVTMLGCCREVGRAAFLLTTPNSRVLIDC
                     GEKPDNSNGTPYLYVPEIHPLAQLDAVVLTHAHLDHCALIPLLYKYGYEGPVYSTPPT
                     RDLSAMLQLDYLDVIHKEDRKVPYSSQEVKTYIRHSITLNYGSVTDIAPDIKLTFHNA
                     GHILGSAIAHFHIGDGMYNIAFTGDFNYSKSRLFNPAINQFPRLEALFMESTYGGSND
                     FQPARSDAEAKLYETINTVLTRGGKVIIPAFSVGRSQEVMLALEEGMRLDKIPKGKIY
                     LDGMIREATAIHTTYPEYLNTELRNLIFREGMNPFLAECFQQVDSQELREKVMSGEPC
                     VIISTSGMLNGGPVMEYLLNLAQDEKNALVFVGYQADGTYGRRIQKGWREVPMGRKGT
                     ITINLEIVTIDGFSGHSDRRQLMNYIGQIQPRPEKIFCIHGDENNTIDLASSIYKRYH
                     IETHSPMNLETYRMV"
     gene            complement(2571..3209)
                     /gene="psmB"
                     /locus_tag="VFC43_00915"
     CDS             complement(2571..3209)
                     /gene="psmB"
                     /locus_tag="VFC43_00915"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit beta"
                     /protein_id="HZK29784.1"
                     /translation="MPEQYQESMKGTTTIGLVFSSGVILATEKRATMGYMIASKKAKK
                     VYQVADRIGMTTAGGVGDAQQLARILTVECNLYQIRRSRPITVGATATLLSNYLNQNR
                     YFPYYVQLLVGGIDETGPSVYSVDAMGGATKEEEIVATGSGSPMAYGVLEDRFVPKMN
                     EDDAIDLAIRGLKSAMKRDAGSGEGIHVVVITKDRYEVLDEDKLKKYLAKTP"
     gene            complement(3299..4147)
                     /locus_tag="VFC43_00920"
     CDS             complement(3299..4147)
                     /locus_tag="VFC43_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZK29785.1"
                     /translation="MIVKHYMTSDVVHVEIPGNRDDVLKILKRTGISGVPVIKNKKIV
                     GIITRKDLLRKPEEVQLGLLMTPKPLTITPDSDIREAARILVSRHIRRLPVVEDGQLV
                     GLLSVADLIHAIAKFKIKDEIKDTYTSLTFALWEETPLPVVGRVMEISGVDAIPILDA
                     ENRLQGIISERDLIRSSSIEDSVGVSDFSNGTDDDEWTWESIRDNHTISFGISKVQLP
                     NRPVKLAMVKNVVAVPVNAEVSECALKMRRARVDQIPVVNGDKRLVAMLYDRMLIRVL
                     CRDPAE"
     gene            complement(4153..4587)
                     /locus_tag="VFC43_00925"
     CDS             complement(4153..4587)
                     /locus_tag="VFC43_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZK29786.1"
                     /translation="MPVFNNILVALDGSEVSQRALVRAADEAKLWNAKLHAIYVVETG
                     LFSSLPLDNTVEIMYRVLEKEGEAVLEKAKKYCSEKGIMVFTHMKQGHAGSEIIALAD
                     REKSDLIIVGSHGKSNADRLLIGSVSTFVVTHSKATTLVVRS"
     gene            complement(4620..5660)
                     /locus_tag="VFC43_00930"
     CDS             complement(4620..5660)
                     /locus_tag="VFC43_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZK29787.1"
                     /translation="MKKNPLIVSGHAFLGEELALLPVDIIIEDGIITAIEESAPVPPV
                     WICPAFFNAHTHIGDTIAMDCGINGDLVALVTPPDGLKHRLLAAASHQDLVRGMHLSI
                     KGMIAGGTAGCADFREGGAGGVSALREAASGLLFQPFIFGREGGQGVAEGLRISSIRD
                     NSSFETIVADARRKGKKIAMHAGEKDAKDIDDAIALEPDLLIHCTHATKKQLRECSDR
                     QIPIAVCPRSNWALGVASSSGHPPLKLMQDLGCRILLGTDNVMFVPPDMFSEMSFTST
                     VYRIDPKVLLRAAIQGSEMTGTSFFIRKGQRANLFTIDTTRSSLCFSRDPLTSLTKRA
                     SSCQIANNVFNL"
     gene            5706..5876
                     /locus_tag="VFC43_00935"
     CDS             5706..5876
                     /locus_tag="VFC43_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit Sec61beta"
                     /protein_id="HZK29788.1"
                     /translation="MAKKQGGRLLSSAGLVNYYESEDRRAVHISPITVIAVAAAIGII
                     IMILNIAYGLQA"
     gene            complement(5915..6664)
                     /locus_tag="VFC43_00940"
     CDS             complement(5915..6664)
                     /locus_tag="VFC43_00940"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HZK29789.1"
                     /translation="MEQIQVMGVRGLPLIHKGDSIAEMICGRAEIQDGDILCIASTIY
                     SKSKGFTKSLASIIPSERACRLATLNGEDPRFVQAVLDASADIIMEHPFILSELSFGH
                     IGVRAGVDQSNIEDGMVIFLPPDPMGAAGEIAKEIRTLVNKNIGVIITDTCGRSFRRG
                     QTGNAIGWSGFYAIRDFRGDTDLFGHVLKITEEAVVDEIAGFANFVMGESNNGVPAVI
                     FRNCPKWTGHDNLYFRTDEDITKRVLKREVR"
     gene            6841..7122
                     /locus_tag="VFC43_00945"
     CDS             6841..7122
                     /locus_tag="VFC43_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1922 domain-containing protein"
                     /protein_id="HZK29790.1"
                     /translation="MYLIIRCPSCRTFTYVDRFQKWKLCPICGHAHEVVKAPAYLEVE
                     HYLEAEHIVKQMEKHLHTTKKPDFSPEETEELRHHYTEALRKKAKGHHA"
     gene            7265..7468
                     /locus_tag="VFC43_00950"
     CDS             7265..7468
                     /locus_tag="VFC43_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29791.1"
                     /translation="MLKRSEGYTDGVIATLPIKTEKQNIAIAMERICPRDRIVATMLD
                     AMPRCLLCTELMMAFVFGDENRA"
     gene            complement(7524..8555)
                     /locus_tag="VFC43_00955"
     CDS             complement(7524..8555)
                     /locus_tag="VFC43_00955"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK29792.1"
                     /translation="MIDRREKKVILLVACLASFLVPFTGSSITVALPAMAAQFNADAV
                     TLGWITSAYIVSAALFIVPFGRLGDIFGRKKLFVLGVLIFTIASLASAFAPTAGILIG
                     ARFVQGIGGAMLFATSVAIVTQVYGPGERGWALGITIATVYAGLSIGPFLGGILIDKF
                     GWPAIFLVNVPVGITTVVLTLREVLHEWADAAGERFDVFGSVVYGTMLFCAIFGTLLI
                     PDPVAGVWIFIAGFALVIFLWWEKRCASPLIDLTIFSGNRTFLFSNIAAMINYGATFA
                     VAVLLSLYLQYIREFSAESAGIILVAQPVIQTIFSPMAGKISDRIEPRIVATIGMASP
                     PPGSHFLSF"
     gene            complement(8633..9763)
                     /locus_tag="VFC43_00960"
     CDS             complement(8633..9763)
                     /locus_tag="VFC43_00960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK29793.1"
                     /translation="MLVNSAGEQKKDQEALLEYKEKYLILTDLMPLGVFRLGPGPEFP
                     VLSANPMLYKMLGYDAEDSLIGISVREIITNPVHWQQIEDDLVSDGAIAGRELQFRQK
                     EGTGILVALNAKSRHAADLQIAWIEGVAEDITERKVLEMEMQYHTTELNRYALSLTQT
                     NKKLNLLSRITRHDILNQLTALAGYLELIREESGDPKIQKYIDVEKRITETIRRQIQF
                     TKDYQEIGIQSPHWYDVKKTIEMAITNLPLPKEALTISIENLSIYADPLLEKVFYNLI
                     ENALRHGNGVSRITFSSYIQEDNINIVCQDNGRGIPEHYKEAIFNRQHFENTGFGLFL
                     SREILGITGLSIHETGEPGKGGRFEITVPPGYFRFDDQPLMP"
     gene            9872..11107
                     /locus_tag="VFC43_00965"
     CDS             9872..11107
                     /locus_tag="VFC43_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZK29794.1"
                     /translation="MDQYDVVVIGAGPAGLFCALQAGMPGHRILLLEKNKKPGEKLLL
                     SGSGQCNITHDGEIREFLSHYGDQGNQVKPALFGFSNRDLIAFFNEHGLPLISENNDK
                     VFPQTRRSADVLSLLVHECRERGVILKCNEPVHRVNRTTQGFEITSTDASYNGRMVVI
                     TTGGMSYPQTGSTGDGYRFSEALGQPLIRTAPALTPLKIRNYPFSALSGISFESMRFT
                     IWRSGVKIADRKGDVLFTHTGLSGPGILDASRNIQAEDEIRLSFVGPLKREEFAADLK
                     KRAEENRNWQLGTLLAAYPIPERLNRKLLLLSKIPPDLKCAHFSTLQRSKLIANCTEF
                     SLIVSELGGFSIAMATRGGVALDGLNMKTMESRIIPGLYFAGEVLDIDGDTGGYNLQA
                     AFSTGFLAAQNIIKNWKIG"
     gene            complement(11231..11668)
                     /locus_tag="VFC43_00970"
     CDS             complement(11231..11668)
                     /locus_tag="VFC43_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HZK29795.1"
                     /translation="MIEIVNKFEKVVYAVLMALLMLVLIGSLLDLVWLLYTAFILQTP
                     GLLESHELIAVLGAFLLVLIGVELLDTIKAYFKENTIHVEIVVLLAIIAIARKVILLD
                     PTGMSGNEFGFELMGIGVIVVGLAAGYYLIKKAGITVEPAKPE"
     gene            complement(11672..12433)
                     /gene="cobA"
                     /locus_tag="VFC43_00975"
     CDS             complement(11672..12433)
                     /gene="cobA"
                     /locus_tag="VFC43_00975"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HZK29796.1"
                     /translation="MNGKVYLVGSGPGGEGLLTQRARVVIDAADVVLYDQLPGKEILA
                     SLPVSAEKIDCGKYGGKHTLEQDEIEALIIDRAKKGKTVVRLKGGDPFLFGRGGEELE
                     SVHSAGIPVEMVPGVSSALAVPASVGIPLTHRKYASQVTILTGNEDPTKPEPALDWQL
                     LAKSRGTIVILMGVANIAKIADVLISNGKESNTMVAIIERGLRKDRRVTTGTLATIAD
                     AARKTGIKPPAVIVIGDVVKLYNPGNPDLVPAEGD"
     gene            complement(12430..13314)
                     /gene="hemC"
                     /locus_tag="VFC43_00980"
     CDS             complement(12430..13314)
                     /gene="hemC"
                     /locus_tag="VFC43_00980"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HZK29797.1"
                     /translation="MSIKVGTRGSKLALAQTQTVCEKLSRLGIDAEQVIMKTQGDTTT
                     GVPLHEIGGQGVFVRALDDAILAGDIDCAVHSMKDIPAFRPSGLFTAAVLKRDSPADF
                     LACTGSSEAIRVIGTSSTRRRAQLLRDNPEITIKELRGNVDTRIRKMTQGEYDAIMLA
                     EAGLTRLGISIPGKRFPPEKFVPAPNQGTIAVVSRADPSLMEELSTLDHPQTRTDVAI
                     ERGAMEHLGGGCFTPLGIYCNEGYLIAEVLSLDGIRTERIETEIKNIEQAREQGRQLH
                     KQAKDLIDDAYRKLGMKI"
     gene            complement(13305..14546)
                     /gene="hemL"
                     /locus_tag="VFC43_00985"
     CDS             complement(13305..14546)
                     /gene="hemL"
                     /locus_tag="VFC43_00985"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HZK29798.1"
                     /translation="MNSSDLFAIAKNLMPGGVSSPVRAIKPYPFYTAHASGSHLTTVD
                     GGSLIDCCMAYGPLILGHAHPQIRKSIETQLKNGWLFGTPSPLEPAFAKIITGDHKGM
                     DMVRFVSSGSEATMAAIRLARGFSQKKDIVKIEGGFHGAHDAVLVKAGSGATTMGVPD
                     SAGVLADLVAHTRQVPYNDAEALESLLVAHDDVAALIIEPVLGNIGPILPRDTYLHEI
                     REITRAHDVLLIFDEVITGYRLGIGGAQKLYKVMPDITTLGKIIGGGLPIGAFGGRRD
                     IMEMVAPQGPVYQAGTFSGNPLSLTAGIATLQWLHDHPTIYRDLEMMTRTLEESLDSV
                     KSGCFVRLGSMFKWFFRDEPPENYRQVKECNTESFGRFWKRMLDKGVFLPPSQFETNF
                     LSSVHSDHDLTTLSQAYQQCL"
     gene            complement(14533..15516)
                     /gene="hemB"
                     /locus_tag="VFC43_00990"
     CDS             complement(14533..15516)
                     /gene="hemB"
                     /locus_tag="VFC43_00990"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HZK29799.1"
                     /translation="MFPQRRMRRSRSKNIQPLLCEITLSKNDLIAPLFVDETITSPLP
                     IESMPGQFRYPVSGIAAVAGELWSQGVRAVLIFGVPKKKDPGGVSAYDPEGVVQKAVR
                     KIKAEVNEMVVITDVCACEYTDHGHCGIIGETRCGTDLLNDPSLELMNKIAISHAING
                     ADVVAPSCMLDGMVGSIRSALDKAGFEDVLIMSYSTKFASALYGPFREAADSGFAFGD
                     RTTYQINCGNSREAILESKMDADEGADILMVKPAGFYLDIVSEVATFGLPVAAYQVSG
                     EYSMIKCAAEKGWIKEKETVLESLIAIKRAGAELIITYFAGDVARWLDEQQ"
     gene            complement(15583..16317)
                     /locus_tag="VFC43_00995"
     CDS             complement(15583..16317)
                     /locus_tag="VFC43_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HZK29800.1"
                     /translation="MPAPLVGRKFGVTVIPPLDPEKRKGIRVLGLSGSSRQQPGMSKS
                     ERLLLGALDTYQEYGCKTSFLRLKDLTIHDCEGNYSENPEYCTYPCQSSLKYDDDQMQ
                     LVYDAVLACDILILATPIRWNNHSAIVQKFVERMNAIENQYSWFGNRMITDKVAAMII
                     VGHVDGVQHVAGNLMNFFSWLGFHTPEVAIASWVGENDEDTTKDWGQIVNNRYTQEDL
                     SNLITSALRLAYALKHHTAEETTASR"
     gene            16425..16691
                     /locus_tag="VFC43_01000"
     CDS             16425..16691
                     /locus_tag="VFC43_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2769 domain-containing protein"
                     /protein_id="HZK29801.1"
                     /translation="MPDDKNSINDENRAKCICRPCPTYNECMRAGDELLFCVTGKSLT
                     CTFDKKACLCPSCPVKPILKLSKAYYCIRGTDKEQNNPVVNKGR"
     gene            complement(16808..18088)
                     /gene="hemA"
                     /locus_tag="VFC43_01005"
     CDS             complement(16808..18088)
                     /gene="hemA"
                     /locus_tag="VFC43_01005"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HZK29802.1"
                     /translation="MNEPVVVPIAIAGVSHHMANVTALETFRFQDEGEFLKTAGQHFK
                     GVLLLQTCNRVELIVEGQVDELHEFLIGLGRKDFFMHDGKGALSHLFSLASGIDSMII
                     GEDQIIGQLKKALADAQESGTASSFLEFCVNKSVHVGIEVRKRTLINRGAVSVGSAAV
                     QLAESEIGSLEGKHILVVGSGEMGLLVAQALSAKQLTAMYVANRTYGRAVILANKIGG
                     KAVHLSELYHYITLSDVVISCTSAPHPIIHKKELAQAMKNRCWPVEGHPRPLILIDIA
                     QPRDVEEGAASIDGVCLFSIDNLRTINEQTMSTRKAEAERAREFVDAEIEIFLRHLNR
                     KSADELLAALHVWADTVRIRERDRAIARIGSSDEKIVEIVDDLSRVLTKKLLTDATFS
                     IRESAEEGDIAAAKAFVKAITRGDRIGSELCRKE"
     gene            complement(18081..18707)
                     /locus_tag="VFC43_01010"
     CDS             complement(18081..18707)
                     /locus_tag="VFC43_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HZK29803.1"
                     /translation="MIPLFVDCSGKRIVIFGGGEVASRKAAYFSGEADIIAVSRSFSH
                     KMSILPIQRRMLDAGRVSDSVLNEIVEGAFLVIGAFSDVGQNNRIRDLCTARGILFNN
                     ADGEPGDVIIPSMTRGENYTIAISTKGSSPAVSRFIREYLETRLPSLDKMVALQRDLR
                     AQLKETEPSVPRRNVILRGVLQDPVIWETLGRDPVQARKYAEERYLHE"
     gene            18971..19042
                     /locus_tag="VFC43_01015"
     tRNA            18971..19042
                     /locus_tag="VFC43_01015"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19004..19006,aa:Gly,seq:gcc)
     gene            complement(19482..>19934)
                     /locus_tag="VFC43_01020"
     CDS             complement(19482..>19934)
                     /locus_tag="VFC43_01020"
                     /inference="COORDINATES: protein motif:HMM:NF019949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FilR1
                     domain-containing protein"
                     /protein_id="HZK29804.1"
                     /translation="DTTTDMFYVYSHYLEILKDASYIHGISSVASPGLAQFIAEKVLT
                     GIPVELVVNNEVIELLSKEPYASNMQGLATYPNFSVWITAEKLRVGLTVTDKYLSLGL
                     FKKGTNLYDSSSDLFSKDERAVEWGETLFLYYKGRSTKLDIAALFQAP"
BASE COUNT         5121 a         4879 c         4693 g         5241 t
ORIGIN      
        1 ataccgataa gtatcaccgc tgagtaccac aaccaccgga attgctgtac gagatacagg
       61 atcgttgcaa aattttcttc acgggtcact gaacccgtct ccctgataaa aaaactcgca
      121 aataccggaa tgataacaag agcagagaaa accaggatgc cggatgcagg attcggaagg
      181 cgtgctgcca gccacccggc acataccagg ccgatcacaa gccctgcgtt aagcgatgcc
      241 atgaggtacc cactcattcg ttcgtgatcg ggaagtgagt tgatatacga catcgcagcg
      301 gcaacaaaga atcccgcgcc tatcccttcg attatgcggg cgataatcac cggaacaggc
      361 aaaaccagaa ccgataacaa aaatccgctg gcaacggtaa ttgcaagccc tgcacggatt
      421 atggggaccc gcccgtaccg gtccgacagg atacctgcgg gaagcgtgct cacaaaagcc
      481 cccaggaaat acgcggagta gatagctgcc tgagccgtgg aagaatcagt gtatgccgga
      541 aggacaggga caattgcatt ggaaagtgcc attacggcaa acatgccaaa aaaaagggag
      601 attcgtgtct tcattaaaag aaccgtcaga ccatgcgata tgtttccagg ttcataggag
      661 aatgcgtctc gatgtgatac cgtttgtaaa tggaacttgc gagatcaata gtattatttt
      721 catcaccatg aatgcagaag atcttttcgg gacgaggctg tatttgaccg atatagttca
      781 tgagttgcct ccggtccgaa tgaccggaaa acccgtcgat cgtgacgatc tcgagattga
      841 tggtaatggt gccttttcgc cccatcggca cttcacgcca tcccttctgg atacgtcgcc
      901 cgtaggtgcc atcagcctgg tagccgacaa agaccagggc atttttctcg tcctgtgcga
      961 ggttcaggag gtattccata acgggtccgc cattcagcat accgctcgtc gaaatgatca
     1021 cacagggttc cccgctcatc actttttcac ggagctcctg actgtcaacc tgctggaaac
     1081 attcagccag gaacgggttc attccctccc ggaagataag gttccggagc tcggtattca
     1141 ggtattcggg ataagtagtg tggatagcgg ttgcctcgcg gatcatgcca tcgagataga
     1201 tcttcccctt cgggatttta tcaagacgca tcccttcttc aagggcaagc atcacttcct
     1261 gggaacggcc gaccgaaaat gccggaatga ttacctttcc accacgggtg agtacggtgt
     1321 taatagtctc gtacagtttc gcttccgcgt ctgaacgggc cggctggaaa tcatttgatc
     1381 cgccatacgt gctctccatg aaaagtgctt cgagccgggg aaactggttt attgccgggt
     1441 tgaaaaggcg gcttttacta tagttgaaat cgccggtaaa agcgatattg tacataccgt
     1501 caccgatatg aaagtgagca attgcggagc cgaggatgtg gccggcatta tggaatgtca
     1561 gcttgatatc cggtgcgatg tctgttacac tgccatagtt cagggttatg gaatggcgga
     1621 tataggtctt gacttcctga gaagaatacg ggactttccg gtcttcctta tggatgacat
     1681 caagataatc gagctggagc attgccgaga ggtcgcgtgt cgggggtgtt gagtagaccg
     1741 ggccttcgta cccgtacttg tacaggaggg ggataagagc acagtggtcg aggtgagcgt
     1801 gagtcagtac cacggcatca agctgggcaa gggggtgaat ctcagggaca taaaggtagg
     1861 gagtgccatt cgaattgtcc ggtttttcac cgcagtcgat caaaacacgg ctgttgggtg
     1921 tcgtaagcaa aaatgcagca cgaccgactt cacggcaaca tccgagcatg gtgacccgtg
     1981 cccactggtc cttttttatc gtgtccttgc cggtatcatc ttttccggga atgtcccggt
     2041 gaatgcgccg acctatcgtc ctgaggaacg acttacgctc ctcgtttacc gaacggaggt
     2101 actggcggac ctgttttacg gttgagcttt cgatgggagg tgtccggaca acttttggtg
     2161 tccacccgat atgtttggtg atctcgcgta gtgtcgcgcc gtttttccct attacaacac
     2221 caggcttttc tgcttctatc agcacttcac cggtatccgc atcaaaaaag atatcggtga
     2281 tacctgctgt gtccggaaca accgatttaa tctcattgta agctttctca gggtcctcta
     2341 atatagtagg tctgacaaca atccgtttcc gcaggtcacg ggcaagtacc ttgatcaagt
     2401 ccgcctcatc ggcaaaccgt ttcggatcat cagtatagat cacaagctcg ggtccttcga
     2461 attcaacctg cgttaccgta atgccgtgag gcactttttc attaatcttg tctttgagct
     2521 ctttgagccg ttcttcaatc agcataaaaa agtcgtccta aaaaagatca ttagggagtt
     2581 ttggcaagat acttcttgag cttgtcctca tctaacacct catacctgtc ttttgtgata
     2641 accacaacat ggataccttc accggacccg gcatcgcgtt tcatcgcgga tttcagccca
     2701 cggattgcga gatcgattgc gtcatcttca ttcatttttg gtacgaaccg gtcctcaagc
     2761 acaccatacg ccatgggaga acccgaaccg gtggcaacga tctcttcttc ctttgttgct
     2821 ccacccatag catcgaccga gtaaacgctt gggccggtct cgtcaattcc cccgacaagg
     2881 agctgtacat aataggggaa gtaacggttc tggtttaagt aatttgaaag cagtgttgct
     2941 gtggccccga ccgtgatcgg acgtgacctg cggatctggt aaagattgca ctctacggta
     3001 agaatacgtg ccagctgctg ggcatcccca actcctcctg cagtagtcat accgatccgg
     3061 tctgcaacct gatatacttt ttttgctttc ttgcttgcga tcatatagcc cattgtcgcc
     3121 cttttctcgg ttgcgagaat tacgccgctg gaaaaaacga gaccaatggt ggtagtccct
     3181 ttcatggact cctggtactg ttcaggcata ttgactcctc aaaaacaaat aaacgctaaa
     3241 atcctgtttt ttaaaagcgc tttgtggtat taaatgatat ggggaaattt aaaggttttt
     3301 attctgccgg atcccggcat aaaacccgga taagcatacg atcatagagc attgctacaa
     3361 gacgcttatc tccatttacg acaggaatct ggtcaacccg tgcacggcgc attttgagtg
     3421 cgcactcgct cacttcagca tttacgggaa cagcgacaac attttttacc atcgcgagtt
     3481 tcacgggacg gttcggaagt tgcacctttg atataccgaa gctgatggta tggttgtccc
     3541 ggatgctttc ccatgtccat tcatcatcat cagtcccgtt ggagaaatcg ctgacaccca
     3601 cactatcttc aatacttgaa ctccgtataa gatcccgctc tgaaataata ccctgcaacc
     3661 ggttctcggc atcaagtata gggatagcat caacgccgga tatttccata actcttccca
     3721 caaccgggag aggggtttct tcccagagtg caaatgtcag gctggtatag gtatccttga
     3781 tctcatcttt tattttgaat ttggcaatcg catggataag atcagctacc gacaggagtc
     3841 caaccagttg gccatcttca acgacaggga gcctgcgaat gtgacgggag accagaatac
     3901 gggctgcctc tctgatatca gaatcggggg tgatagttaa gggttttggt gtcatgagga
     3961 gcccgagctg gacctcttcg ggctttctca acagatcctt tcgtgtaata atgccgacga
     4021 tctttttatt cttgataacc gggacaccgg atatacccgt ccgcttgagt attttgagga
     4081 catcatcccg gtttcccggg atttcgacgt gaactacatc ggatgtcatg tagtgtttga
     4141 caatcatgtc tgttatgatc tcaccaccag tgtcgttgct ttgctgtggg taactacaaa
     4201 ggtgcttacg ctgccaatga ggagtctgtc agcattgctt ttaccatgcg aaccgacgat
     4261 aatcaggtca gatttttccc gatcggcaag agcgatgatt tcacttccgg catgtccctg
     4321 tttcatatgg gtaaagacca tgattccctt ttcggagcag tatttttttg ctttttcaag
     4381 tactgcctct ccctcttttt cgagaacccg gtacataatt tccacagtat tatccaaggg
     4441 caaggaagag aacaggcccg tttcaacaac gtatatggca tggagcttgg cgttccataa
     4501 ttttgcttca tcagcagccc tgaccagcgc gcgctggctt acctccgatc catcaagtgc
     4561 aacaagtata ttattaaaca ctggcatccc ccaaaacaaa catggctctt ttattggatt
     4621 tacaaattaa aaacgttatt ggctatttgg caggaagaag ccctttttgt caaggaagtc
     4681 agcggatcgc ggctgaaaca aagagatgat cgtgtggtat ctatcgtaaa caaattcgcc
     4741 cgctgacctt tgcggataaa aaaggaggtc ccggtcattt cagagccctg gattgcggca
     4801 cgaagcagga ctttgggatc aatccggtaa accgtggacg taaaagacat ttcagaaaac
     4861 atatccggtg ggacgaacat cacgttgtcg gtaccgagga gtattctgca cccgagatcc
     4921 tgcattagtt tcagaggcgg gtgccctgat gaagaagcaa caccgagtgc ccagttggat
     4981 ctgggacaga cagcaatcgg tatctgtcgg tcagaacatt cacgaagctg tttttttgtt
     5041 gcatgcgtac agtggatgag aaggtctggt tcaagcgcaa ttgcatcatc aatatctttt
     5101 gcatcctttt caccggcatg catggcaatt tttttccctt tacggcgagc atccgcgaca
     5161 atggtttcaa agctgctgtt atccctgatg ctgctgatcc taaggccctc ggcaactcct
     5221 tgccccccct cacgcccgaa tatgaagggc tggaataata atccagaggc tgcttctctg
     5281 agtgccgata cacctcctgc tcccccttca cgaaagtctg cacagccggc ggtaccaccg
     5341 gcaatcatac ctttgatact caaatgcatc ccccgaacaa gatcctgatg tgaagcagca
     5401 gcaagcaagc ggtgtttcag accgtctggt ggagttacca gggcaacaag atccccgttt
     5461 ataccgcaat ccattgcaat ggtatccccg atgtgggtat gcgcattaaa aaatgcagga
     5521 cagatccata ctggcggtac aggagcagat tcttcaatgg ccgttataat accgtcctca
     5581 ataataatat caacgggtaa tagtgcgagt tcctctccaa ggaaagcatg tcctgacact
     5641 ataagagggt tttttttcat gggtcgtaac tctcttttat atgttgaaaa atgaaatata
     5701 tttgcatggc aaagaaacaa ggcggcagat tactctcatc ggctggtctc gtcaattatt
     5761 acgagagcga agaccggcgg gctgttcata tcagtcccat taccgttatc gcagtcgcag
     5821 cagcgattgg catcattatt atgatcctga atatagccta tggattgcaa gcgtagatct
     5881 ccttaaaaat aaatcctatt tctcagtatt tatctcatcg cacttctctt ttgaggacac
     5941 gcttcgtgat atcctcatct gtgcggaaat agaggttatc atgcccggtc cacttcgggc
     6001 agttgcggaa gattactgca gggacaccat tgttgctctc acccatgaca aagtttgcaa
     6061 agccggcaat ttcatcaacc actgcttctt cggttatctt gagaacatgc ccgaatagat
     6121 cagtgtcgcc acggaaatcc cggattgcat aaaatccgct ccatcctatt gcattccctg
     6181 tctgcccacg gcggaatgat ctcccgcagg tgtctgttat aataacccca atattcttat
     6241 tgaccagcgt tctgatctct ttagcaattt cccctgcagc gcccattgga tccggaggca
     6301 gaaaaattac catcccgtcc tcgatattac tctggtcgac tccagcccgc acgccgatat
     6361 gaccaaaaga gagttctgac aggataaaag ggtgttccat tattatatct gcggaggcat
     6421 cgaggacagc ctgaacaaaa cgggggtctt caccgttcag ggttgcaagc cggcaggccc
     6481 gttctgaagg aataatagat gcaagagatt ttgtgaaacc ttttgatttt gagtaaatag
     6541 ttgaggcgat gcagagaatg tcgccgtcct gaatctctgc cctaccacaa atcatctcag
     6601 cgatgctgtc acccttatga atcaggggaa gccccctcac acccataacc tgaatctgct
     6661 ccatagtacc atcagtatga gaagatccga tattaaagga gtttcgtttc gcatgccatc
     6721 tggatatatg gataattcat cttttttgag gacagcgtta tatacctgtg aaaggtatcc
     6781 ttgagaatct atcaatcacc gatatgtttt tatcaggata acacgcatca cctctttgtt
     6841 atgtacctta ttatccggtg cccttcctgc agaactttta cctatgtgga tcgtttccag
     6901 aaatggaagc tctgtcctat ctgcgggcat gcccatgagg tggtcaaagc accggcgtac
     6961 cttgaggttg agcattatct agaggccgaa catattgtaa aacagatgga aaaacacctc
     7021 catacaacaa agaaaccgga tttttcaccg gaagaaaccg aggaacttcg ccatcattat
     7081 acggaagcac tccgaaaaaa ggccaaaggc catcatgcct gattatcttt gattttttgc
     7141 ctcattcaat gaggtttgat tttcaatacc ggctggtatg tgagatgtat ggatagaacc
     7201 ccgcacgtat gatgcaccaa ccccgataat acaaaaacac gaaaaaatca ggaatgcggt
     7261 agttgtgctg aagaggagcg agggatacac cgacggtgtt attgccacac tcccgataaa
     7321 aacagaaaaa cagaacattg ctatcgccat ggagaggatc tgtccgagag atcgcatcgt
     7381 cgcgaccatg ctcgatgcga tgccaaggtg ccttctgtgt actgaactca tgatggcatt
     7441 cgtattcggc gatgagaaca gagcataacc gaatccaagt accataaggc agattatgat
     7501 cgcaaagagt ggggttgtcg gagttaaaaa gataaaaaat gagagcccgg cggtggtgat
     7561 gccattccga ttgtggctac gatacgtggt tcaatacggt ccgatatctt tcctgccatt
     7621 ggtgagaaaa ttgtctggat gaccggctgg gcgacaagta tgatcccggc cgattctgct
     7681 gaaaattccc ttatatactg aagatagagt gagagaagaa ctgccacagc aaaagttgca
     7741 ccatagttaa tcattgccgc aatattggaa aacaggaaag tccgattgcc ggaaaatatg
     7801 gtgagatcga tgagcgggct ggcgcagcgt ttttcccacc agagaaaaat gacaagtgcg
     7861 aatccggcaa tgaatatcca tactcctgcg acaggatctg gaataaggag agtaccgaaa
     7921 atagcacaga ataacatggt gccatatact actgacccaa agacatcaaa tcgctcaccg
     7981 gcagcatccg cccactcatg gagcacttcc ctgagggtaa ggacaactgt ggtaatacct
     8041 actggtacat tgaccaggaa gattgccggc cacccgaatt tatcaataag aatgcccccg
     8101 aggaacggtc ctattgaaag accggcgtag acggtagcga tagtgattcc cagtgcccag
     8161 cctcgttcac ccgggccata gacctgagtg actatagcaa ctgatgtggc aaagagcatt
     8221 gcgccgccga tcccctggac gaaacgtgca ccaatcagga tgccggcagt gggagcgaaa
     8281 gcagatgcaa gtgatgctat ggtaaaaatc aggacaccca gtacaaacag tttttttcgc
     8341 ccgaaaatat ccccgagtct cccgaaaggc acgataaaaa gagcagcgga aacaatatat
     8401 gccgaagtta tccaacccaa agttaccgca tctgcattga actgagctgc cattgcgggt
     8461 aatgcaacag tgatagatga tccggtaaaa ggtacaagaa atgaggcaag gcatgcgaca
     8521 agaagaatga ccttcttttc gcgtctgtcg atcatgagta tacccgggtt agtaatgata
     8581 tggattaaat atattgaatt taaagggcta aaaaaacaaa cgacgaagat cttcagggca
     8641 ttagtggctg atcatcaaaa cggaaatacc ccggtgggac cgttatctcg aatctcccgc
     8701 ctttaccagg ttcgccggtt tcgtgaatag agagacctgt aatacccaga atctcccgcg
     8761 acaggaagag tccgaaaccg gtattttcaa aatgctggcg attgaagatc gcttctttgt
     8821 aatgctctgg aatacctctg ccattgtcct ggcatacaat gttgatattg tcttcctgaa
     8881 tgtaggatga gaatgtaatc cttgacactc cattgccatg tcgcaatgca ttctcgataa
     8941 ggttatagaa aactttctcc agcagaggat ctgcgtaaat actgagattt tcaatgctga
     9001 ttgtcagcgc ttctttggga agaggaaggt ttgttattgc catttcaatg gtttttttca
     9061 catcatacca atgaggggac tggataccaa tttcctgata gtccttggta aactggatct
     9121 gtctcctgat agtttctgtg attcttttct caacgtcaat atatttttgg attttaggat
     9181 cccctgactc ttcacgtatc agctcgagat agcctgcaag tgctgtcagc tggttaagaa
     9241 tgtcatggcg ggtaatacgg gagagaaggt ttagtttttt atttgtctgg gtgagtgaca
     9301 gtgcgtaccg gttcagctct gtggtgtggt actgcatctc catttcgagc acttttcgct
     9361 cagtaatatc ttctgctact ccttcaatcc aggcaatctg aagatcagct gcgtgccggc
     9421 tctttgcatt gagtgcaacc agaattcctg ttccctcttt ctgccggaac tgcagttcac
     9481 gcccggcgat ggcaccatcg ctgaccaaat catcttcaat ctgttgccaa tggaccggat
     9541 tggtaataat ttcccgtacg gaaattccaa tgagtgagtc ttcagcatcg tatcccagca
     9601 tcttatacaa cattgggttg gctgataaga ccgggaactc cgggcctgga ccaagacgga
     9661 aaacacccag gggcatcaaa tcagtcagaa tgagatattt ctctttatac tcaagaagtg
     9721 cctcctgatc ctttttttgt tccccggcac tgttaaccag catctacttt tttttattcg
     9781 tatgaattta ttaataatct ttcattttgc atggaataat ccggcgaaat acctaaacga
     9841 atataccccc ttctaatctt ataggagatt tatggaccag tatgatgtgg tagtgattgg
     9901 tgcaggcccg gccggactgt tctgtgcgtt acaggccggg atgcccgggc accgtattct
     9961 cctgcttgag aaaaataaaa aacctggtga aaaacttctc ctttcaggct ccgggcaatg
    10021 caatataacc cacgatggtg agattcgtga gttcctttcg cattatgggg atcagggaaa
    10081 tcaggtgaaa ccggctttgt ttggtttttc caaccgtgat ctcatcgcat tctttaatga
    10141 acacggactt cccctgatat cagaaaacaa tgataaagta ttcccccaaa cacgccgttc
    10201 ggcggatgtc ctttcgctgc ttgtacatga gtgccgggaa cgaggtgtaa tcctgaaatg
    10261 taatgagcca gtacataggg tcaaccgtac aactcaggga tttgaaatta cttctacgga
    10321 tgcaagctac aacggaagga tggttgtcat cactaccggg ggaatgtcgt accctcagac
    10381 tggatctaca ggtgacgggt accggttttc tgaagctctg ggacaaccgt tgatccgaac
    10441 agcccctgct cttactcccc tgaaaatcag aaattacccc ttttcagcac tttccggcat
    10501 ctcgtttgag tctatgcgat ttaccatctg gcgctccgga gtaaagatcg cggatcgtaa
    10561 gggtgatgtt ctgttcacgc ataccggatt gtcgggccct ggaatcctcg atgcttcgcg
    10621 gaatattcag gcagaagatg aaatcagact ttcgtttgtc ggtcctttga aacgcgaaga
    10681 gtttgcagct gacttaaaaa aacgtgccga agaaaacagg aactggcaac tcggcaccct
    10741 cctcgctgcg tatccgattc cggaacgatt gaacaggaaa cttttattat tatccaaaat
    10801 tcctccggat ctcaaatgtg cacatttttc aacacttcaa cgatcaaaac tgattgccaa
    10861 ctgcacagag ttttccctga ttgtttcaga acttggaggc ttttcgatcg caatggccac
    10921 aaggggcggg gttgcactgg atggattgaa tatgaaaaca atggaatcaa ggatcatacc
    10981 cggtttatat tttgccggtg aagtgctgga tattgacggt gataccgggg gatataattt
    11041 gcaggcagca ttttccaccg ggtttttggc agcacaaaac ataattaaaa actggaaaat
    11101 tggttgagac ccgctgtttt tttattattc atacaatttg tgatttacct tgaaatcaac
    11161 caaagttaga acatgagtat atttcgtaca ggatcatttt ccttttccag gtgatatggg
    11221 aaaaaaataa ttattccggt ttagccggct cgactgtaat gccggctttt ttgatgaggt
    11281 aatatcctgc agccaggccg accacgataa cacctatacc catcaactca aaaccaaatt
    11341 cattgccgct catgccggtg ggatccaaca ggatcacctt ccgtgcgatc gcaatgatag
    11401 caagaaggac aacaatctcc acatgaatgg tattctcctt aaagtatgcc ttgatcgtat
    11461 ctaaaagttc gacgccgatc agcaccagca ggaaggcgcc gagcacagca atgagttcat
    11521 gtgattccag caaaccgggt gtctgaagga taaatgcagt ataaaggagc catacaaggt
    11581 caagcaggga cccgattaat acgagcatta gcagggccat gagcactgca tatacgacct
    11641 tttcgaattt attgacgatt tcaatcatgt tttaatctcc ttcagcaggt accagatccg
    11701 gattacccgg gttatataat tttactacat ccccgataac gattacagcc ggaggtttaa
    11761 tgcccgtttt tctggctgca tcagcaatgg ttgcaagtgt tccggtagtg acccggcggt
    11821 cttttcgtaa accccgctcg attatcgcaa ccatggtatt tgattccttg ccgttgctga
    11881 taagtacatc cgctatcttt gcgatatttg ctactcccat cagaattact attgttcccc
    11941 ggctctttgc aagtaactgc cagtcaagtg ccggttccgg ctttgtcggg tcttcattgc
    12001 ccgtcagaat tgttacctgt gatgcatatt tccggtgtgt aagtggaatt cctaccgatg
    12061 ccgggacagc gagcgcgctg ctcaccccgg gtaccatctc aacaggtata ccggcgctgt
    12121 ggactgattc cagttcctct cccccgcgtc cgaagaggaa gggatcgcca cctttgagcc
    12181 tgaccacggt tttgcctttt tttgcccgat caatgatcag ggcttcgatt tcgtcctgtt
    12241 cgagtgtatg tttaccccca tactttccac aatcaatttt ttctgcactc acgggtaacg
    12301 atgcaagaat ttcctttccc ggaagctggt catacaacac gacatcagca gcatcgataa
    12361 cgactctggc ccgctgcgtg agaagtcctt ctccgcccgg acctgatccg accaggtata
    12421 ctttaccgtt catattttca ttcccagttt ccggtatgca tcatcaatca ggtcttttgc
    12481 ctgtttatgg agttgccttc cttgttcacg ggcctgttca atatttttaa tttccgtttc
    12541 aattcgttcg gttcgtatac cgtcgaggga aagtacttca gcaatcaggt aaccctcgtt
    12601 gcaataaatg ccaagtggtg taaaacatcc cccgccgaga tgttccatag ccccccgttc
    12661 aattgccaca tcagtgcggg tctggggatg gtcgagtgtg gagagttcct ccatcagtga
    12721 gggatcggcc cggctgacga cagcaatcgt tccctgattt ggtgccggaa caaatttttc
    12781 aggaggaaat cgcttccccg ggattgaaat acccagcctg gtcagcccgg cttctgctaa
    12841 catgatggca tcatattccc cctgggtcat cttccttatc ctggtatcga catttccccg
    12901 aagctccttt atggtaattt cgggattatc gcgaagcagc tgggcacgcc ttcgtgtgct
    12961 ggaggttccg atcactctga tggcttccga agatcccgta caggcaagga aatcggctgg
    13021 tgaatcgcgc ttgagaacag ctgcagtaaa gagcccgctt gggcggaaag ccgggatgtc
    13081 tttcatactg tgaacagcgc aatcaatatc gccagccagg attgcatcat caagtgccct
    13141 gacaaagaca ccctgcccgc caatctcatg gagaggcacc ccggttgtgg tgtcgccctg
    13201 ggtcttcata ataacctgct cagcatcgat tcccagcctc gacagttttt cgcagaccgt
    13261 ttgcgtctgt gccagtgcca gtttgctgcc ccgtgttcca acttttatag acattgttgg
    13321 tacgcctgtg aaagagtagt gagatcgtga tcgctgtgca ctgaagaaag gaagtttgtt
    13381 tcaaactggg aagggggtaa aaataccccc ttatccagca tccgcttcca gaaccggccg
    13441 aaagattccg tattacactc tttgacctgc cggtaatttt ccggaggttc atcacggaag
    13501 aaccacttga acatagatcc gagccggaca aaacatccgc tctttacact atcaagagat
    13561 tcttcgagag tgcgtgtcat catttcgaga tcccggtaga tggttggatg gtcgtgcagc
    13621 cattgcaggg tcgcgatacc tgcagtgagt gacaacgggt tacctgagaa ggtcccggcc
    13681 tggtacaccg gtccctgtgg tgcaaccatc tccattatat cgcgccgtcc gccaaatgca
    13741 ccgatgggaa gtcctccgcc aattattttt cccagtgtgg taatatccgg cattaccttg
    13801 tacagtttct gggctccccc gataccaaga cggtaaccgg taattacttc atcaaaaatc
    13861 aggagcacat cgtgtgcacg ggtaatttcc ctgatttcat gcaggtaagt atcccgggga
    13921 agaatcggac cgatattccc gagcaccggt tctatgatga gtgcggcaac atcatcatgt
    13981 gcgacaagga gtgattccag agcttctgcg tcattgtagg gtacctgacg ggtatgggct
    14041 accaggtcag caagaacacc cgccgaatcg ggtacaccca tggtagttgc ccctgatccg
    14101 gctttgacga gaaccgcatc gtgggcacca tgaaatcccc cttcgatttt cacgatatct
    14161 tttttttgtg aaaaaccccg tgcaagacgg attgctgcca tagttgcctc agaaccgctg
    14221 gaaacaaacc gtaccatgtc catcccctta tgatcaccgg taataatctt tgcaaaagca
    14281 ggttcaagag gtgaaggagt accaaagagc cacccattct tcagttgtgt ttcgattgat
    14341 ttcctgatct gagggtgtgc atgtccgaga ataagtgggc cataagccat gcagcagtca
    14401 attaatgaac cgccatcaac tgttgtcaga tgcgatccac ttgcatgggc agtgtaaaac
    14461 gggtaaggtt taatcgccct caccggactg cttacacctc ctggcatgag gttttttgct
    14521 attgcaaaca agtcactgct gttcatcgag ccacctcgca acatcgccgg caaaataggt
    14581 gatgatgagc tctgcgccgg cacgttttat ggcgatcagg ctctcaagaa cggtctcctt
    14641 ttcctttatc cacccttttt cagctgcaca tttaatcata gaatattccc cgctaacctg
    14701 atatgcggca accgggaggc cgaatgtggc aacctctgat acaatatcga ggtagaaacc
    14761 tgccggtttt accatgagaa tatcggctcc ttcatctgca tccatctttg attcaaggat
    14821 cgcctcgcgg gaatttccac aattgatctg gtatgttgtc ctgtctccaa atgcgaaccc
    14881 cgaatctgca gcctcgcgaa aaggcccgta aagagcggaa gcaaactttg tggaatagga
    14941 catgataagg acatcttcaa agccggcttt gtcaagggcc gaacggatcg agccaaccat
    15001 gccatcgagc atgcacgatg gtgcgacaac atcagccccg tttatcgcgt ggctgattgc
    15061 aattttattc ataagttcga gcgaaggatc attgagaagg tcagtgccac atcgtgtttc
    15121 accgattatc ccgcaatgac catgatcagt atactcgcac gcgcagacat cggttataac
    15181 aaccatctca ttaacttctg ctttgatctt ccttaccgcc ttttgcacaa ctccttccgg
    15241 atcataggca gaaacgccac ccggatcctt ttttttggga acaccaaata tcaggaccgc
    15301 cctcacaccc tgtgaccata attctccggc aactgctgca atgccactga ccggataacg
    15361 gaactggccg ggcattgact cgatcgggag gggagacgtg atagtttcat caacaaaaag
    15421 cggagcaata agatcatttt ttgaaagggt gatttcgcac aggagcggct gtatattttt
    15481 tgaccgcgat cgcctcatcc ttcgttgggg gaacatagtt agcgcaccat ctcctgtagt
    15541 cgttgtttac cttttcttaa atcgattcta tttatggcta ttttatctgc tggcggtggt
    15601 ttcttctgcg gtatgatgct tgagggcata tgcaagcctc agcgcgctgg ttatcaggtt
    15661 gctgagatcc tcctgggtat accggttatt cacaatctgc ccccagtctt ttgtcgtatc
    15721 ttcatcattt tcaccgaccc aggatgcgat ggcaacctcc ggagtgtgga aaccaagcca
    15781 tgaaaaaaaa ttcatgagat taccggctac gtgctgcaca ccatcaacgt gacccacgat
    15841 aatcatcgcc gctactttgt cagtgatcat acggtttccg aaccatgaat actggttctc
    15901 gatggcattc atccgctcaa caaatttctg aacaatcgca gagtggttat tccagcggat
    15961 aggtgttgcg aggatcagga tatcgcatgc cagtacggca tcatacacga gttgcatctg
    16021 gtcatcatcg tacttcaacg agctctgaca gggataggta caatattcag gattttcgga
    16081 atagttaccc tcacaatcgt gaatagtgag atcttttaac cggagaaaac tggtcttgca
    16141 cccatattcc tggtatgtat caagcgctcc gaggagcagg cgttcagatt tactcatacc
    16201 gggttgctga cggctcgaac ctgaaagccc aagtacacga attccctttc gtttttccgg
    16261 gtccagcggc gggatgaccg tgacaccaaa ttttcgtccg acaagtggtg caggcatgat
    16321 ttaattatca aggagcccgc gataatctag ttattgatct acatctccgg aatatcactc
    16381 ctgtcctgca ggtatgttaa accggagcag ggagctaaga tagtatgcct gatgacaaga
    16441 acagcatcaa tgatgagaac cgggcaaagt gtatctgcag accgtgcccg acatataacg
    16501 agtgtatgcg ggccggggat gaactattgt tctgcgtaac gggtaaaagt ctgacctgta
    16561 cctttgataa aaaagcatgt ctctgtccga gttgtcctgt aaagccaatc ctgaaacttt
    16621 caaaagcgta ttattgtatc cggggtacag acaaggaaca aaataaccct gtggtcaata
    16681 aagggagata gaaataaacc ggaaaaaaat aatgaagact ttctcttttt attatcggga
    16741 ggtcttttga atcctgcgta cgggatactt tatcaatctg tcagtgtagt aaagaaaccc
    16801 gttccattca ctcttttctg cacagttcac tgccgatcct gtctccacgg gtgatggctt
    16861 tgacaaaggc ctttgcggca gcaatgtctc cttcctctgc actctcgcgg attgagaagg
    16921 ttgcatcggt cagcagtttt tttgtcagaa cccggctcag gtcatcgaca atctcaacta
    16981 tcttctcatc agaagaacct atccgggcga tagcacgatc gcgctccctg attcgcacgg
    17041 tatcagccca cacgtggagt gcagcaagta attcatctgc tgacttccgg tttaagtgac
    17101 gcaggaaaat ttcgatttct gcatcaacga actcacgtgc ccgctcagcc tcagccttcc
    17161 gggtactcat ggtttgttca tttatcgtac gcaggttatc gatggaaaac aggcagacac
    17221 catcgatgga agcggcgccc tcctcaacat cacggggctg ggcgatatcg ataaggataa
    17281 gcggtcgcgg gtgtccttcg actggccagc agcggttttt cattgcctga gccagttctt
    17341 ttttatggat gatcgggtgt ggtgcagatg tgcaggaaat gacaacgtcc gagagggtga
    17401 tatagtggta tagttccgac aggtgtactg cttttccccc gatcttattt gcaagaatga
    17461 ctgcccgccc ataggtcctg tttgcaacat acattgccgt cagttgcttg gctgaaagag
    17521 cctgggccac cagcagaccc atctcaccac tgccaaccac gaggatatgc tttccctcaa
    17581 gactaccgat ttctgattcg gcgagctgta cagctgcaga gcctacggaa actgcgcccc
    17641 ggttaattaa tgtgcgtttg cgcacctcga tccccacgtg gactgacttg ttgacgcaga
    17701 attctaaaaa agagcttgcg gttcctgatt cctgcgcatc agccagggct tttttcagct
    17761 gcccgatgat ctgatcttca ccaatgatca tggaatctat cccggatgcc agactaaaga
    17821 gatgggaaag tgctcctttc ccgtcatgca taaaaaaatc cttgcgtccg agtccgatca
    17881 gaaattcgtg caattcatcg acctgtcctt cgacaatcag ttcaacccgg ttacatgtct
    17941 ggagtaaaag aacacctttg aaatgctggc cggctgtttt aagaaattcg ccctcatcct
    18001 gaaaccggaa tgtttcaagt gcggtgacat ttgccatgtg atgacttacc ccggctatcg
    18061 cgatggggac tacaacaggt tcattcatgc aggtatctct cctctgcata tttgcgagcc
    18121 tgtactggat cgcggccgag tgtctcccag attaccggat cctggaggac cccccgaaga
    18181 atcacattac gccgtggtac agagggttcg gtctctttta actgggctcg caggtcgcgt
    18241 tgtaatgcca ccatcttatc aagggaaggc aacctggtct caagatattc ccttatgaac
    18301 cgggatactg cgggactgga tcctttcgtg ctgatcgcaa tcgtgtaatt ttcacctctg
    18361 gtcattgaag ggatgatcac atccccgggt tcaccatccg cgttattaaa caggatgcct
    18421 ctagcagtgc agagatccct gatccgattg ttctgtccta cgtcagaaaa tgcaccgata
    18481 accagaaacg ccccctcaac tatttcatta agcacactgt ccgatacgcg gcctgcatcc
    18541 agcatccggc gttggatggg aaggatgctc atcttgtggg agaatgaacg gctcacggct
    18601 atgatatcgg cctctccgga gaaatacgca gcttttcgtg atgcgacttc acctcccccg
    18661 aatataacga tgcgcttacc cgaacaatcc acaaagagag ggatcatctg gtattagata
    18721 gagttattac gttactttaa gatgttggaa ttgttgcgat ctctcctgct ttctcccagg
    18781 agaaggtttg aatataatgg ggaaacacca gaaaaaaccg gttttctggt tggttccgga
    18841 aaactcttcc ggggacatga gaggtggatg agtaaaaggc aaatcaggaa aaagcatcca
    18901 aaggtgtgtt tgtgccccgg aagttcgata tacttaaatt gtatgaatgc tcacacttta
    18961 gagcacattt gcgccgatag tgtagtggtt atcactaggc gttgccaacg cctaaacccg
    19021 ggttcgagtc ccggtcggcg catccatatt gactcgtttt tgagagctgc tctaactcaa
    19081 tatcttaaaa aggctctaaa tagaaatcag ggcacaattc agagatattt atattgcagc
    19141 tgctcccaaa aaccggtctt attgggcact ttttatcacg agttttccag ataactgaaa
    19201 taagggcata gaattcaaga ataatccatt taatgggaga aaaaccggcc tttttgttcc
    19261 cttttggagg gcagatctta tgttagatca ttaaccagcc aacctgttct ctcgggcaga
    19321 aaaaatgacc cttaccgggg tctctacgct attcggctgt gtgccctcgt aaaatccgga
    19381 ttaatgagac cggaatgtac cggatctgtc ctgtgaactt cttaatcagg gttctctggt
    19441 aaaaaaaatc tggataaaaa ttcccagaaa ccaggctgta cttatggtgc ctgaaataat
    19501 gctgcaatat caagtttggt tgatcgtccc ttatagtaca ggaaaagagt ctctccccac
    19561 tccactgcac gctcgtcttt gctgaaaaga tccgaggaac tgtcatagag attagtgccc
    19621 ttcttaaaga ggccaagtga gaggtactta tctgtcacag tcagcccgac ccggagtttt
    19681 tctgccgtta tccataccga aaaattagga taggttgcga gtccctgcat attcgatgca
    19741 tagggctctt ttgataaaag ttcaatgact tcattattta ccacaagttc gaccggaatg
    19801 ccggtaagga ctttttctgc aatgaactgg gcaaggccgg gactcgcaac ggatgaaatt
    19861 ccatggatat atgaggcatc tttcaggatc tcaagatagt gagaatacac gtaaaacatg
    19921 tcggttgtcg tgtc
//
LOCUS       DASRLV010000027         3787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_91677,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000027 DASRLV010000000
VERSION     DASRLV010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91677"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VFC43_01025"
     CDS             <1..182
                     /locus_tag="VFC43_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29805.1"
                     /translation="AIFMKGYGKKTKLGLYVSREILAMTGIQIDETGEEGKGAKFELT
                     IPKEGYRFSSGSSKK"
     gene            452..1270
                     /locus_tag="VFC43_01030"
     CDS             452..1270
                     /locus_tag="VFC43_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZK29806.1"
                     /translation="MGTILVDQDLCTRCGICSVICPMSIVEPADENTLPKVLDVKAGM
                     CIQCGHCESYCPTQALLLNVRPDEKVLIQPGAGTLSAEDLEVYLKKRRSVRHYTNDPV
                     SKETITHMLDIARYAASGSNGQPVEWIVVHDQKKVQKIAGLTVDWMKTLLNTSHPMSG
                     YVPMFIKAWESGHDVICRGAPHLLFAHIPESNPHTLVDAIIAMTHFDIAAPSFGIGTC
                     WAGFVAAASREYPPLKEALDIPAGRSIAYAMMFGHPHYKVSGIPRRKPLQVTWQ"
     gene            1302..1949
                     /locus_tag="VFC43_01035"
     CDS             1302..1949
                     /locus_tag="VFC43_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HZK29807.1"
                     /translation="MKVLAFNGSPRKKWNTATLLQNALDGAASQGAETELVHLYDLTY
                     TGCTSCFACKLKGGKSYGKCAVRDELTPVLEKIPDADAIILGSPIYFGTVSGEMRSFM
                     ERLLFPYLLYSNPPSTLFPKKIKTAFFYTMNVSEHQMKEFGYPLHVASNANILARTFG
                     YTESLFSFETLQFEDYDKVVFSYFDPEERRKRRRTIFPKDCERAYELGARLGKNA"
     gene            1971..2540
                     /locus_tag="VFC43_01040"
     CDS             1971..2540
                     /locus_tag="VFC43_01040"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HZK29808.1"
                     /translation="MIKVTILIGSPRKHGSTRVLAEEAERGLREQGVETEFIFLNDLK
                     FRGCQACYYCKKNDVAACAVQDDMQKIYPLMQESAGLIVATPIYFGGVTAQTKTWLDR
                     LFPYIGMNLSPKLPKSKKVSFIFTQKQPDVHLFEKSLTQFMHMVALTGFSLKDYLIAY
                     DLDAGVNQCVTGKKEFMEKAYRIGKDLLT"
     gene            2677..3762
                     /locus_tag="VFC43_01045"
     CDS             2677..3762
                     /locus_tag="VFC43_01045"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HZK29809.1"
                     /translation="MTISYLTLERFRNQQLGGNISMRAEEIVAWLGAVQAQDYAGAEW
                     SIGLRLPGSTEGDIEKAVADRKIVLTWALRGTLHFVAAPDIRWMLALLAPGIIAKRAS
                     RYRQLGLDKKTFAECKQVLIQELQGDRQRTRKDLISSLEDAGISTEGQRGYHLLGRSG
                     LEGVICFGPKEDRQQTFVLLDAWIPPSPDQDHLTSMAGLAGRYVASHGPATLQDFIWW
                     SGLSAADARSALAAAGPDLYTDSLGGRVYWRSKSNFPSHHASPKNVLLPAYDEYLLGY
                     RDRSHALDPGDAGKVNLIKGLALPILIDGKVVGTWKRTPKQNKILIALDYFHHPKEDE
                     QSAILAAAERYGEFIGKSVEVQHSYHP"
BASE COUNT          996 a          948 c          983 g          860 t
ORIGIN      
        1 aagccatctt tatgaaagga tacgggaaaa agacaaaact cggactctac gtttcccgcg
       61 agatcctcgc catgaccggt attcagatag acgaaacagg agaggaggga aaaggagcaa
      121 agttcgagct taccatacca aaggaaggat accggtttag ctcaggatct tcgaagaaat
      181 aaaaaacagg aaaatctccc gggagatatc atccccgtag tcagtttttt gagtttgaaa
      241 atatacgaaa aatccattca gcactgatta ttttcaaata ccttcacctc ataaaaagcc
      301 gggccagggt tttgtctttt ttttcttacc ggggtccctg agaagatatt gatttttcgc
      361 acacagtcgt tttaattacc tgggaacact tttttaaaac cgtgcattga ttatcagata
      421 agttaggaat agtactccat ggagtgaaaa aatgggaacg atacttgttg accaggatct
      481 ctgcacccgg tgcgggatct gctcagttat atgcccgatg tccatagtcg agcctgcaga
      541 tgagaacacc cttccgaagg tgctggatgt aaaagccggt atgtgtatcc agtgcggtca
      601 ctgcgaatcc tattgcccca cacaggcact gctgctgaac gtccggccgg atgagaaggt
      661 gctcatacag ccaggtgcag gaaccctgtc agcagaagac ctcgaagtgt acttaaaaaa
      721 gcgccggtct gtccgccact atacaaacga ccctgtatca aaggagacga ttacgcatat
      781 gctggatatc gcccggtacg ctgcctcggg gagcaacggg cagccggtag agtggatcgt
      841 ggttcacgac cagaaaaaag tgcaaaagat tgccggtctt accgttgact ggatgaagac
      901 actcctcaat accagtcacc cgatgagcgg gtatgtcccg atgtttatca aggcatggga
      961 aagcggtcac gatgtgatct gccgtggcgc cccgcacctg ctctttgccc acatacccga
     1021 gtccaatccc cacacattgg tcgacgccat cattgcgatg acgcatttcg atattgctgc
     1081 gccttcgttt ggaatcggga cgtgctgggc gggttttgtc gcggctgcat cccgggaata
     1141 ccccccgctc aaagaagcac tggatatacc ggcagggcgt agtatagcct acgcgatgat
     1201 gtttgggcac ccgcactaca aagtcagcgg gattccccgc aggaaacccc ttcaggtaac
     1261 ctggcagtaa accagacagt cagatacagg agaaagcgac aatgaaggtc cttgcattca
     1321 acggaagtcc gcgaaagaaa tggaataccg caaccctcct ccagaacgca ctggacggtg
     1381 ccgcgtccca gggtgcggaa acggaactgg tccatctcta tgatctcacg tataccggct
     1441 gcaccagctg ttttgcctgt aaactgaaag gcgggaagag ttatggaaaa tgtgcggtca
     1501 gggatgaact gactcccgtc ctggaaaaga ttcccgatgc cgatgcaatt atccttggct
     1561 ccccgatcta ttttgggacc gtgtccggtg agatgcgttc ttttatggag cgtctgctct
     1621 tcccgtatct cctgtacagt aatcccccgt caactctctt tccaaaaaag atcaaaacgg
     1681 ccttttttta cacgatgaac gtctcggaac accagatgaa agagttcggg tatcccctgc
     1741 acgtggcttc aaatgcgaac atccttgcac ggacattcgg gtatactgaa tccctcttct
     1801 cctttgagac actccagttt gaggattatg acaaggtcgt attcagttat ttcgatccag
     1861 aagagcgccg taaacggcgg agaacgattt tcccaaaaga ctgtgagcgt gcgtatgaac
     1921 tgggggcacg gctgggaaaa aacgcgtgag gaggagaagc gagaagaacc atgataaaag
     1981 taacaatatt aatcggcagc cctcgcaaac atggcagcac ccgtgttctt gcagaggaag
     2041 ccgaacgggg gctcagggaa cagggggttg aaacggaatt tatcttcttg aacgacctga
     2101 aattcagggg gtgccaggcc tgctattact gtaagaaaaa cgatgtggcc gcctgcgcag
     2161 tacaggatga tatgcagaag atctaccctc ttatgcagga atctgccgga ctcattgtcg
     2221 caacacccat ctacttcggg ggagtgacgg cccagacaaa gacatggctt gaccggcttt
     2281 tcccctatat cgggatgaat ctgagcccga aactgccaaa atcaaaaaag gtatcgttta
     2341 tctttaccca gaaacagccg gatgtccacc tgttcgaaaa atccctcact cagttcatgc
     2401 acatggttgc cctgaccgga ttctcgctca aagattatct gattgcgtat gacctcgatg
     2461 ctggggtaaa tcaatgcgta acgggcaaaa aggagtttat ggaaaaggca taccggattg
     2521 gaaaagacct cctcacataa gttttttcat gtcggacctg gacatgccga tggacaaaat
     2581 tttgtgtgcc gaaaaccggc aaggctgctt cgctccacat gacaattacc tgaaccggtt
     2641 ctccgttgac gatcagtgac ctgaaaaaag gggaaaatga ctatttctta tcttactctg
     2701 gaacgcttca ggaaccagca gctgggcggg aacatatcca tgcgggccga agagatagtt
     2761 gcatggctgg gagccgtaca ggcccaggat tatgccggtg cagaatggtc aatcggtctc
     2821 aggttgccag gcagcactga gggggatata gaaaaagcag tggcagacag gaaaatcgtc
     2881 ctgacatggg cactccgggg gacattgcat tttgttgctg caccggatat ccgctggatg
     2941 cttgctcttc ttgcgccggg catcatcgcg aagcgtgcct cacggtatcg ccagcttgga
     3001 ctggacaaaa agacatttgc ggaatgcaaa caggttctta tccaagagct tcagggtgac
     3061 cggcaacgga cccgcaaaga cctgatctct tctcttgaag atgctggtat atccacagaa
     3121 gggcagcggg gatatcacct gctgggacgg tccggattgg agggggtaat ctgttttgga
     3181 ccaaaggagg accgacaaca aacgtttgtg ctcctggatg cgtggatacc cccctccccg
     3241 gatcaggatc atctgacatc aatggccgga cttgccggcc gttatgttgc cagccacggg
     3301 ccggcaacac tgcaggattt catctggtgg tcaggtctgt cagctgctga tgcacgatca
     3361 gcccttgctg cagccggccc tgacctgtat acggattccc tcggtggccg ggtatattgg
     3421 cgatcgaaat cgaactttcc ctctcaccac gcatcaccaa aaaacgtcct ccttcctgca
     3481 tatgatgagt acctgctcgg gtacagggat cgctcccatg cccttgaccc cggggatgcg
     3541 ggtaaggtga atcttatcaa agggctggcc cttcccattc ttatcgacgg caaagtggtg
     3601 ggtacatgga aacgaactcc caaacaaaat aagatcctga tagcactgga ttactttcat
     3661 cacccaaaag aggatgaaca gtccgcaatt cttgctgctg ctgagaggta tggtgaattt
     3721 attggtaaat ctgtcgaggt acaacactcg tatcacccgt gaagctcccg atgagacggg
     3781 atgtacc
//
LOCUS       DASRLV010000028         9884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_494658,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000028 DASRLV010000000
VERSION     DASRLV010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 9884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9884
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494658"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1518)
                     /locus_tag="VFC43_01050"
     rRNA            complement(52..1518)
                     /locus_tag="VFC43_01050"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01959"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01959"
     gene            complement(2201..3868)
                     /locus_tag="VFC43_01055"
     CDS             complement(2201..3868)
                     /locus_tag="VFC43_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZK29810.1"
                     /translation="MLLFLLALFQPALALRDVRVGMHEIKPSLYTDEQGNPAGIFVDL
                     IQDIAPKEGWNLIYVHGTFRENQERLANGQIDLIMALTDTPEREKIYDFNREAAVSSW
                     TQIYASPHAGINTILDLDKKRVAILRGDVNAIAFRENAEKFNINPTYIEQDDLSGVFQ
                     QVKNGSADAAVASRIAGQLYEQQYGVVTTSVMFYPNPLGFAVPKGKNQDLILAIDSYL
                     AREKGDPSSYYSQTMQKWFGEKAGWVIPPYIWWGLTGFAVLAALFVVMSVFLQREVRR
                     KTAELSKQNTELQSEITSRTLAEAELVRKNEELQAAYEELTATEEELRANYLEIGKSK
                     QSLLQARKKLNFLNTLTFQDIQNGVFSVSGFIELAKSSGCSEGAKEYLGRGMASIRSV
                     ESSLRFAKNYQDLGISQPKWQNVNNVFLFAISHLDLSHLSRTVNLDGVRIYADPLLEK
                     VLFSLMENVIRHGKGATSIALHYQKTPEGLSILIEDNGPGIPAAAKEKIFERGYAGKG
                     ESGLFLAQEVLSLTGISIRETGVEGTGARFEILVPEGTYQFPTKDRV"
     gene            complement(4074..4964)
                     /locus_tag="VFC43_01060"
     CDS             complement(4074..4964)
                     /locus_tag="VFC43_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HZK29811.1"
                     /translation="MADPGGEEPGNHHHENSKKDPVISEPGELRPEDPSGTNAESLPV
                     LEPEPSGSPIQGIIEDPVEILVGLAERGEIDPWNINIIEVTDRFLSELERCRQLNLQV
                     SGRTLFYAATLLRMKSEHLELLSAPTGADPDEDDGLSGEDYDVPLDSEIDYGGRLGPI
                     ERLEHEIQRRLDRKNMRKSPVTLFELIIELKNIEKEERRRRRLTDTPDDLLIYAEDVV
                     GIAHDEGYQESSMVRLAEYLEGLEIDEEMTLAELCKQLEWGIPEVYIPLLFLALDGRC
                     SLRQEEFFGDIFVQICRAGE"
     gene            complement(4957..8400)
                     /gene="smc"
                     /locus_tag="VFC43_01065"
     CDS             complement(4957..8400)
                     /gene="smc"
                     /locus_tag="VFC43_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HZK29812.1"
                     /translation="MHITELEIDNFKSFSKKTKIPFLEGFSVISGPNGSGKSNIIDSI
                     LFVLALSSSRNLRAEKLTDLINLNSGRNTAEVALEFSDGTKIKRRIKRTGNGYYSYNY
                     LNDRLCKQSDIVNHLAKHGIKPHGYNVVMQGDVTRIMEMSDFERRKIIDEIAGVSEFD
                     IKKQQSLSELDIVRERIEREELLLLELTKRVNELKREREHALEYQKWQKDLLFFQNCR
                     AAAQLHEREKELASLLRSTDEHKILLTRTSADRSLEENELSYVKADLQDIDDLINKKS
                     GSDYLKLIAELEEAKSGIKLAEQTIVRLRKEKETNLEAINRVYMDTKRAEARVAECTD
                     QIRTLSIDRTNIAMEIAGARAQLEKLETEMKQHGEDTEGAREKLFSLLKEIEEKKGQR
                     SEILHQQDMLIEKSRMRTTELERLTHLLLQLDEEYAVKQTQFSDGEKSVSDLLLEKKD
                     LDRTLSELEGTVFAQRSSLERLRAEIREYEQDAFRLEAAQQARGESGGRAIEAVMAME
                     HVHGTIAELGKSPAEYSTALNTAAGNKLQFVVCDDDQVAADAIQYLKAEKLGRVTFLP
                     LNKLKPPALPPLKEPGVIDYAVNLVEYDPRYDRAFAVTLGATVVVDTLERARKLIGKY
                     RMVTLEGELLEKSGAMTGGATKKQGGRGFGAAVDDEIIRIRSHLAEIQGEAATLESGI
                     KRLTEEVDKQRAKRNEIDQNIARQGMFTEEFSRRFEAITVEKQTIEAAVARQKEETGN
                     GAAELTLLEADLDKTTEAINAIQSQVEEIKKRLDDTNIPVLSDQIEKKRKEIEESERR
                     LRNKEADINDAMRERQHFTSRLTELGEDRKRQDERNGQIDADITGFNEQIASCKSQIA
                     ALEERQKEFSGELDELRTRRSETSRQIQESELKLMKFDAEKERITIQMVAIEERARTL
                     GIEVDMLRHQVGDLDTNLSLLEIEGRIAEADGALRKIGAVNMLAIEEYEKVQRQVEER
                     TERKDTLSHERTTLIERIEKFEQMKYEAFTTAFHAIDTNFREIFARLTSGSGHLILEN
                     EEDPFAGGMTFAVQPRDKKVHLLSSLSGGEKSLTTLAFIFSIQRYIPAPFYAFDEVDM
                     SLDGSNVERIASMITELAPQSQFVIVSLRKPMIDAAQRIMGVTLRPDKSTLVTGVKAN
                     G"
     gene            complement(8413..8898)
                     /locus_tag="VFC43_01070"
     CDS             complement(8413..8898)
                     /locus_tag="VFC43_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29813.1"
                     /translation="MLEISGTGPATEERIAAIEKKMRDIEALVKGLTQELLDLKSLAM
                     KMNKQTEERSRQELKRVQAVVQDAPAPTAAPGGALGSTVVMRKGARASDAPQAPPEPV
                     MDMIMQSDGTMKMEQRRGDKNYIVASAGYGKNKKGTSVKQKQGDLIVAAEEEKKDPAK
                     K"
     gene            9209..>9884
                     /locus_tag="VFC43_01075"
     CDS             9209..>9884
                     /locus_tag="VFC43_01075"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866455.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HZK29814.1"
                     /translation="MSYLSKTDPEIADIIEKERLRQVNGLELIASENVVSRAVLEAMG
                     SIMTNKYAEGYPGKRYYGGCEFHDMAENLARNRLKQLFGAEHANVQPHSGTQANMAVY
                     FAYMKLGEKILSMKLNQGGHLSHGSPVSFTGKFYQVAQYGLDPKTEMLDYGAVEEMAK
                     AEKPRIIVCGASAYPSTIDFKTFQSIADGVGAYCMAYIAHIAGLVATGLHPTSVGVVN
                     FTTTTTH"
BASE COUNT         2211 a         2647 c         2309 g         2717 t
ORIGIN      
        1 caattttaca gtttgtttag caaccgcgta acccaaaaaa tcaatttcgt taggaggtga
       61 tccagccgca gattccccta cggctacctt gttacgactt aacccccctt gcaaaaccta
      121 gattcgaccg ccgcaaaaac gacggcctca tcaagacctc actcgggtgg tttgacgggc
      181 ggtgtgtgca aggagcaggg acatattcac cgtgccatgt tgagacacga ttactacaga
      241 ttccagcttc atgtgggcga gttgcagccc acaatccgaa ctaaggatgg gtttaggaga
      301 ttgccttcac ctttcggtgt cgatacccat tgtcccaccc attgtagccc gcgtgtagcc
      361 cggacaattc ggggcatgct gacctaccgt tgcccattcc ttcctcctct ttagcagagg
      421 cggtcccaac agtgtcccca ccatcccgga ggacgagctg gcaactgttg gcgtgggtct
      481 cgctcgttgc ctgacttaac aggatgcttc acagtacgaa ctgacgacgg ccatgcacct
      541 cctctcagct agtcaagtag agtcttcagc ccgactatca ttcagctgtc ttgtccggtg
      601 agatttccgg cgttgagtcc gattaaaccg caggctccac ctgttgtggt gctcccccgc
      661 caattccttt aagtttcagc cttgcgaccg tacttcccag gcggcacgtt tcacgatttc
      721 tcttcggcac ctcggtgact cgtggtcacc gatacaccta acgcgcatcg tttacggctg
      781 ggactacccg ggtatctaat ccggtttgct cccccagctt tcgtccctca ctgtcggagc
      841 cgttctggtg agacgccttc gccacaggtg gtcccccaag gattacagga tttcactcct
      901 acccctggag tacctctcac ctctcccggt ccctagtctg ccagtatccc tgaaacgcct
      961 attggttaag ccagtagatt tccccagaga cttaacagac cagctacgaa cgctttaagc
     1021 ccagtaatag tggccaccac tcgagccgcc ggtattaccg cggcggctgg caccggtctt
     1081 gcccggccct ttcttcacct gttatttaaa cagatggaca gcccgatttt acgggcactc
     1141 ggagttccct tatcacggtt tcccgcattg taaagttttc gcgcctgctg cgccccgtag
     1201 ggcctggatt cgtgtctcag aatccatctc cgggctcttg ctcccacaac ccgtaccgat
     1261 tacaggcttg ttgggccgtt accccaacaa caacctaatc ggccgcagac ccatcctatg
     1321 gcggcggacc tttcaactac aaagcattcc agcattcata gtctatgggg tattatcccc
     1381 agtttcccgg ggttatcccc ctccataggg taggttgtcc acgtgttact gagcagtatg
     1441 ccgaggtctt gcacctctcg actcgcatgg cttaatcgaa ctccgatagc agtggcctct
     1501 ggcaggatca accagaatta attgtacaat gtacgcacac tttagttact gaactgtttt
     1561 aaggaattag cggttactaa acaaatttcc gcattgccag tatcaacgtc agaaccaact
     1621 ccggtcaagc cggatatcgt tggatctcca tcaaagagaa tttgtatggt atttttcgtc
     1681 gtgattgctt acttaatcac gggctaataa cataggctgt ggtattattt ataccttgtt
     1741 gccttcacaa tttttttaat atcagaaccg acaccggtct tttcagacgg tttttttcgg
     1801 ttcttcatca gatcttgtgg atcacataat tgcacactca aacacttaaa cataacccat
     1861 gaaaatgtat cgctttttct cccttgatgt atgagctgaa aattatacat aaaaaaaata
     1921 acttctgaat ggttcaatat taaaatttca acagcaattc tggcattttt agtatacaga
     1981 aagggtttcc gtaaaaatgt cattctgagg aaaaaaagaa tatcctgaaa tcatcctcta
     2041 tagcagagcg cgttttcgct catggacttt aactgacggt aaacccggtt tttgtttatg
     2101 ttttaaaaaa accctgccgc gatcgcgcag taaatatttt ttcccaattc cacggcatat
     2161 tgctaatatc agccggatta ccaaaagtat aggacctgat tcagacccga tccttcgttg
     2221 ggaactggta ggtaccttcc ggcacgagga tctcgaacct ggccccggta ccttcaacac
     2281 ccgtctcccg aatagagatt ccggtaaggg atagtacctc ctgggcaagg aacaacccgg
     2341 attcgccttt ccctgcatat ccgcgttcga aaatcttttc tttggctgct gccgggatgc
     2401 cgggtccatt atcttcaata aggatcgaaa ggccctccgg ggttttctgg taatgcaggg
     2461 caatgctggt cgcccctttc ccatgcctga tgacattttc catcaggctg aacaggacct
     2521 tctcaagcaa tggatctgcg tatattctga caccgtcaag attcacggtt cgtgacagat
     2581 gggataaatc caggtgcgaa atcgcaaaca aaaaaacatt gttcacgttc tgccatttgg
     2641 gctggcttat tcccaaatcc tggtaatttt ttgcaaagcg cagggagctt tccacagaac
     2701 ggatagatgc catacccctc ccgagatatt cctttgcacc ttcactgcac cctgaacttt
     2761 ttgcaagctc gataaaaccg gaaacagaaa atactccatt ctgaatatcc tggaaggtga
     2821 gcgtgttcag gaagttgagt tttttccttg cctgcaaaag agactgtttg cttttcccga
     2881 tctcaagata gtttgcccgc agctcctcct cagtcgcggt cagttcctcg tatgctgcct
     2941 ggagttcctc gtttttccgg accagttccg cttctgccag cgtgcggctg gtaatctctg
     3001 actggagttc cgtgttctgc ttggaaagtt cagcggtctt gcgcctgacc tcccgctgaa
     3061 ggaaaacgct catcacaaca aagagggcag cgagaacggc aaacccggta aggccccacc
     3121 agatgtaagg cgggattacc cagcctgctt tttccccaaa ccatttctgc attgtctggc
     3181 tgtaataaga agacggatcg cctttttctc ttgcaaggta gctgtcgatc gctaaaatga
     3241 ggtcctggtt ctttccctta gggacggcaa aaccgagggg atttgggtag aacattaccg
     3301 aagtggtaac aacaccgtat tgttgttcat agagctggcc tgcgatacgg cttgcaactg
     3361 ctgcatcagc cgatccgttt tttacctgct ggaaaacacc actcaggtcg tcctgttcga
     3421 tatacgtggg gttgatattg aatttctctg cattttcccg gaaagcgatc gcattgacat
     3481 ctccccggag aatggcaaca cgttttttgt caagatcaag gatggtattg atacccgcat
     3541 gcggtgacgc atagatctgg gtccatgacg atacagcagc ctcacgattg aaatcgtaga
     3601 tcttttcgcg ctcgggggta tcggtaagcg ccataatgag gtcgatctgg ccattggcaa
     3661 gacgctcctg gttttcacgg aacgtgccgt gaacgtagat gaggttccag ccttcttttg
     3721 gggcaatgtc ctggatgagg tcgacaaaaa ttcccgcggg atttccctgt tcatcggtat
     3781 aaagcgaagg cttgatctca tgcatcccaa cccggacgtc acgcagggca agagccggtt
     3841 gaaaaagtgc aagaaggaag agaagcacaa tcgccaaaaa aaataccctt cgggatccat
     3901 gaaacgtggg ggcgtaattc ccgaaatgag ggtgcatgaa tcgaccagac cttgggttct
     3961 gaaattcccg gcaagggatg gtttcagtgt ttgatttcgt taatacattg gtagagattc
     4021 atgtatatca tttttggtac cagaaacaat aaaaaccgtc ggaatgggct tcctcactct
     4081 cccgcccggc aaatctgtac aaaaatatcc ccaaagaact cttcctgccg cagtgaacaa
     4141 cggccatcaa gagcaaggaa gagaagaggg atgtagactt ccggaatgcc ccactccagc
     4201 tgtttgcaca actccgcaag cgtcatctct tcatcgatct ccagcccttc aaggtattct
     4261 gcgagccgca ccatggacga ttcctgatag ccttcatcat gtgctatacc gacaacatct
     4321 tcggcataaa tgaggagatc atcaggtgta tccgtgagcc gtcggcgccg gcgctcttct
     4381 ttttcaatgt ttttaagttc tatgatgagc tcaaagagcg ttaccgggct ttttctcatg
     4441 ttcttccggt cgagccggcg ttgaatctcg tgttccaggc gctcgattgg acctaacctt
     4501 cccccataat cgatttcaga atcaagcggt acatcataat cttcgccaga caacccatca
     4561 tcttcatccg ggtctgctcc tgtgggtgct gagagcagct cgagatgttc tgacttcata
     4621 cgaagaaggg ttgcagcata aaagagcgtg cgcccggaaa cctggagatt gagctgccgg
     4681 catcgttcga gttcagacag gaaccggtca gttacttcta tgatattaat attccaggga
     4741 tcgatttcac cccgctcagc aagacctaca aggatctcga ccggatcttc gataattccc
     4801 tgaatgggtg agcctgaagg ttccggttcc aggacaggga gagattccgc attggttcct
     4861 gacggatctt ccgggcgtaa ttcaccgggt tcagaaatta ccggatcttt tttggaattc
     4921 tcatgatgat ggttccccgg ttcctctccc ccgggatcag ccatttgctt ttaccccggt
     4981 aacaagagtg ctcttatccg gtcgcagggt aacacccatt attctctgtg ccgcatcgat
     5041 catgggctta cgcagagaga caatgacaaa ttgtgactgg ggagcaagct cggtgatcat
     5101 cgatgcaatc cgctcgacat tagagccgtc aagcgacatg tccacttcat caaacgcgta
     5161 gaatggtgcc gggatatacc gctggatgga gaagatgaac gcaagcgtgg taagcgattt
     5221 ttctcctccg gagagcgatg agaggagatg tactttttta tcacggggct ggactgcaaa
     5281 tgtcatgcca ccggcaaacg gatcttcctc attctcgagt atcaggtgcc cgctcccact
     5341 cgtcaaccgt gcaaatattt cacggaaatt ggtgtcaatg gcgtgaaaag cagtggtaaa
     5401 cgcttcgtac ttcatctgct caaatttctc tatccgctca ataagagtcg tccgttcgtg
     5461 ggacagggta tcttttcgtt cggttcgttc ctccacctgc ctctggactt tctcgtattc
     5521 ctcaatggcc agcatattca ctgccccgat cttacggagc gccccgtcag cctctgctat
     5581 cctgccttca atttccaaaa gggacaggtt tgtatccagg tccccgacct ggtgccggag
     5641 catatccact tcgattccga gggtccgggc acgttcctca attgccacca tctggatcgt
     5701 gatccgctct ttttcggcat cgaatttcat taactttaat tcggattcct ggatctggcg
     5761 ggaggtttct gacctgcggg tacgcaattc gtcgagttcg ccggaaaatt ccttctggcg
     5821 ttcttccagc gcggcaatct gtgatttgca ggatgcaatc tgctcgttga accccgtaat
     5881 atcggcatcg atctggccgt tgcgttcatc ctgcctttta cggtcttcac cgagctcagt
     5941 caggcgggaa gtgaagtgct gccgctcacg cattgcgtca ttgatatcag cctccttgtt
     6001 tcgcaaccgt ctttcggact cctcgatttc tttgcgcttt ttctcgatct ggtcggaaag
     6061 cacgggaata ttggtatcgt caagacgttt cttgatctcc tcaacctggg actgtatcgc
     6121 gttgatcgct tctgtggttt tatccaggtc agcctcaaga agggtgagtt ccgcagcgcc
     6181 gttaccggtc tcttcctttt gacgggccac agctgcttca atagtttgtt tttcgacagt
     6241 gatagcttca aacctgcggg aaaactcttc ggtgaacatg ccctgccggg cgatattctg
     6301 gtcgatctcg tttcgtttcg ccctttgttt atccacttct tcggttaagc gttttatacc
     6361 cgattcaaga gtcgccgctt ctccctggat ttctgcaagg tgcgagcgga tccttatgat
     6421 ctcgtcatca acagcagctc cgaaaccccg gccgccctgt ttctttgtcg cacctccggt
     6481 cattgccccg cttttttcca gcagttcacc ttcaagcgtt accatgcggt attttccgat
     6541 aagttttcgt gccctctcga gagtgtcaac gacaaccgtc gctccgagcg taacagcaaa
     6601 cgcccggtca tatctgggat catattcgac aaggttgaca gcgtaatcga taacacccgg
     6661 ctcttttaac gggggaaggg ccgggggttt taatttattg agcgggagga aggttacccg
     6721 gcccagtttc tcggctttta agtattggat cgcatctgct gcaacctgat cgtcatcaca
     6781 gacaacaaac tggagtttgt tccccgctgc agtgttcagc gcagttgaat attcagcagg
     6841 ggatttcccg agttcggcga ttgtaccgtg aacatgttcc atcgccatga ctgcttcaat
     6901 tgccctgccc ccggattctc cccgtgcctg ttgtgcggcc tcgagccgga atgcatcctg
     6961 ttcgtattcc cggatctccg ccctgaggcg ttccagcgat gaccgttggg caaataccgt
     7021 accttccagt tccgacagcg tgcgatcgag atcttttttc tccaaaagaa gatcggacac
     7081 acttttctca ccatcggaaa actgcgtctg cttgacggca tattcttcat ccagttgcaa
     7141 aagaagatgg gtgagtcgtt caagttctgt cgtgcgcatc cggctttttt cgatcagcat
     7201 gtcctgctgg tgaaggattt cggatcgctg gcctttcttt tcctcaatct cctttaacag
     7261 ggagaacaat ttttcccggg ctccttcagt atcctccccg tgctgtttca tctccgtttc
     7321 aagtttttcc agctgggcac gggctccggc aatctccatg gcgatgtttg tgcggtcaat
     7381 ggaaagcgta cggatctggt ctgtgcattc tgccacccgt gcttcagcac gtttcgtatc
     7441 catatagaca cgattgattg cttcgagatt ggtctctttt tccttccgga gccgcacgat
     7501 tgtctgctca gcgagtttga tcccgctttt agcttcttcg agttcagcga taagtttgag
     7561 ataatctgac ccgctcttct tgttgatcag gtcatcgata tcctgtaagt cggccttgac
     7621 gtaggacaat tcattctctt caagactccg gtcagcactg gtacgggtga gcagtatttt
     7681 gtgctcatct gtcgatcgca ggagcgatgc aagttctttt tcccgttcgt ggagttgggc
     7741 tgctgcccgg cagttctgga aaaacaagag atccttttgc catttctggt attcgagagc
     7801 atgctcccgc tcccgtttga gctcgtttac cctctttgtc agctcaagca ggagaagttc
     7861 ttctcgttca atccgttccc tcacgatatc aagttcagag agcgactgct gtttttttat
     7921 gtcaaattct gaaacgcccg caatctcatc gataatcttc cgacgctcga aatcgctcat
     7981 ctccataatg cgggtgacat cgccctgcat gacgacatta tagccatgag gttttatccc
     8041 gtgctttgca aggtgattca cgatatcgct ctgcttgcag agccgatcat ttaagtaatt
     8101 gtagctgtaa tagccatttc ccgttcgctt gattcttctt ttgatctttg tcccgtcaga
     8161 aaattcgagt gctacttctg cggtgttcct gccggagttg aggttgataa ggtcggtgag
     8221 tttctcggcc cggagattcc gtgaactcga gagtgcaagc acaaaaagaa tagaatcgat
     8281 aatgttgctc tttcccgaac cgttgggccc ggaaataacg gaaaatccct ctaaaaaagg
     8341 gatttttgtc tttttagaaa aagacttgaa attgtcaatc tccagttccg tgatatgcaa
     8401 agatccggct ccttacttct ttgcaggatc ctttttttct tcctcggcgg caacgataag
     8461 gtcgccctgt ttttgcttga cagacgttcc tttcttattt ttcccgtatc cggccgatgc
     8521 aacgatataa tttttgtctc cgcgccgttg ttccattttc atggtcccgt ctgattgcat
     8581 aatcatgtcc ataacgggtt ccggtggggc ctgaggagcg tcagatgccc gtgctccctt
     8641 gcgcatgacg actgtgctac ccaacgctcc acccggtgct gcggtgggag ctggtgcatc
     8701 ctgtactact gcctgaacgc gtttaagctc ctgcctgctg cgttcttcag tctgtttgtt
     8761 cattttcata gccagtgact taaggtcgag aagttcctgg gttaacccct tcacaagagc
     8821 ctcgatatcc cgcatctttt tttcaatcgc tgcaatacgc tcctcagttg caggtccggt
     8881 tcctgaaatt tccagcattc gtatcagttt ctcctttgtg atcgtttgtg ctcaagatat
     8941 atgatctttg catcaggctg cgcctgtgat ctgatccagt actggtgcag cgcactcctt
     9001 ataaataata gtagttatcg ccaaataagg aaaaaaggtt tgtgctctat ttcatctgat
     9061 actccgttgt gtgcgtataa aacctgaatt atttctcact ccgttcacgc gcactatgat
     9121 aaaaaaaacg atacggctgc acttcccatc aacacgctcg ccagtatcgg gaaaaaggta
     9181 ttactgattg aagcgaaccc ttctccatat gtcctatctg tctaaaactg atccagaaat
     9241 agcagatatt atcgagaaag agaggctccg ccaggtcaat ggcctggaac tgattgcatc
     9301 tgaaaatgtt gtttcccgtg cggttcttga agcaatggga tccatcatga ccaataaata
     9361 tgccgagggc tatcccggca agcgatacta tggtggatgc gagttccacg atatggccga
     9421 gaaccttgcc cgcaaccggc tgaagcagct cttcggggca gagcatgcga acgtccagcc
     9481 gcactcgggc acccaggcaa acatggccgt ctattttgcg tacatgaaac tgggggagaa
     9541 gatcctgagc atgaaactca accagggagg tcacttatcc catggctccc cggtcagttt
     9601 taccgggaag ttctaccagg tggcacagta cggcctcgat cccaaaaccg agatgctcga
     9661 ttacggcgca gttgaagaga tggcaaaggc ggaaaaaccc cggatcattg tctgcggtgc
     9721 ctcagcatac cccagcacaa tcgacttcaa gacgttccag tcgattgctg acggtgtcgg
     9781 tgcatactgc atggcataca ttgcccatat cgccggtctt gtcgccacgg gcctccaccc
     9841 cacctctgtc ggcgtggtca actttactac caccaccacc caca
//
LOCUS       DASRLV010000029        16808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_495088,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000029 DASRLV010000000
VERSION     DASRLV010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 16808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16808
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495088"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..123
                     /locus_tag="VFC43_01080"
     CDS             <1..123
                     /locus_tag="VFC43_01080"
                     /EC_number="2.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107638.1"
                     /note="Required for the methylation step in diphthamide
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine synthase"
                     /protein_id="HZK29815.1"
                     /translation="RAGSAQVVRSVDFGPPLHILVVPAELHEMERTYLEMFAGL"
     gene            120..695
                     /locus_tag="VFC43_01085"
     CDS             120..695
                     /locus_tag="VFC43_01085"
                     /inference="COORDINATES: protein motif:HMM:NF015943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF357 domain-containing protein"
                     /protein_id="HZK29816.1"
                     /translation="MKIAECQVLLTRTLSATYVSAPKGTHFYRIAAQILEMARAYESD
                     GIRFFQTGDPVNALASYYYGFGWLHFGVSSGLLNIPCPAACPFKGPAEVLHIRHRSKL
                     EEKTTRYARLLDIARSSVKCSMDPATTSSVFARRMLCIAGTYARRGEYLVNAGDPEGA
                     LASFSYGHGWLDAAVTAGFFCIVAERDLFTI"
     gene            835..1854
                     /locus_tag="VFC43_01090"
     CDS             835..1854
                     /locus_tag="VFC43_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HZK29817.1"
                     /translation="MEKSQVKWLYISVGFSLLVLVIILYFTINENTVYYLRQINPVFL
                     FLAFMTHILTMCFWALRIKKMSGSLGYCIGFFYSLNLVFANLLAAAITPAQAGGEPVR
                     VHELYRANVPIGDATAIVIMERALDGIVLAALAAFAMIVLTDQWESLGAISEIMVYIT
                     WVFVAGCLILFYLAVKRPDVVKRVVNRIACFFTRTWENARVEKFILRADKEIDNFNDA
                     VIKFVRSAKGGLLWGLVFTLLYWVSEILTASLILVGLGQPPLILESFVIQLILAILMM
                     LPLTPGSSGIAEAGAYSMYALFIPVSIVGIFVVIWRIVLYYFNIALGILSSIIIVRRE
                     AQCKE"
     gene            1915..2367
                     /locus_tag="VFC43_01095"
     CDS             1915..2367
                     /locus_tag="VFC43_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HZK29818.1"
                     /translation="MTQVRCEVKGVFVAMSDATTIPVVLLTDGGQLSLPIFIGIWEAV
                     SINSAKNKEILSRPFTHDLFLDLAAKFSITFHSLQIDSIEDGVYYAQLVLSADHHREF
                     IDCRPSDGIALALRADIPIFVDKDVLESAGKKFSDLPPLVDLSTFLRK"
     gene            complement(2370..2672)
                     /gene="hisE"
                     /locus_tag="VFC43_01100"
     CDS             complement(2370..2672)
                     /gene="hisE"
                     /locus_tag="VFC43_01100"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HZK29819.1"
                     /translation="MKDNVDPAIISEIWSVIQERANNPSAESYTSRLLTDPKGIDKVL
                     EKVGEESIEFILAAKNGIKKRTTEEASDLLFHLLVALCAAGIDLSDVMQELQKRRK"
     gene            complement(2669..3112)
                     /locus_tag="VFC43_01105"
     CDS             complement(2669..3112)
                     /locus_tag="VFC43_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusA-like transcription termination
                     signal-binding factor"
                     /protein_id="HZK29820.1"
                     /translation="MERNIGFKERRYIEELRILTKSTALDCVIDDRFDRVIYVIRQGD
                     MGLAIGKKGDNIRRLQSVLGKRIEMVEYAETPEEFIMNIFKPAEVVRIERGAENGPLN
                     VFVKQRSDLGIAIGKSGCNIEKARILSRRFFGSDVGEVLLIQEDA"
     gene            complement(3268..4002)
                     /locus_tag="VFC43_01110"
     CDS             complement(3268..4002)
                     /locus_tag="VFC43_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284803.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HZK29821.1"
                     /translation="MMKKFSLLTVAIVIVAMTCIVSVLAAPADTAAVNVIHATGSGNV
                     IGTPDRAQVTFSVVTENPDVRTAQQANAVQMKKVIDALGAAGLPEDALKTTGYNIYPL
                     YDDYSKSPFEQKIRTYQVTNTLTVTLHDVNRTGEVIDIAVKNGINQASSIQFMLSDEQ
                     SQTLRTEALKKAVSRARADADTVAGALGTTILGVQSVDISGGYSPVLFENYKVDNAVS
                     APTTPTPIQPGDVTVNAQVSITYTIR"
     gene            4134..5384
                     /locus_tag="VFC43_01115"
     CDS             4134..5384
                     /locus_tag="VFC43_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HZK29822.1"
                     /translation="MGATIVEKIFSRKCGRDIRAGEVVMTPLDGAMIHDITGPLAIQK
                     FYEMGGTKVFDPRRVIMLFDHQIPADSIAAAENHVYMRRFAVEQQIHNYDIREGVCHQ
                     VVVEKGRAAPGEIVVGADSHTCMYGAAGAFATGVGSTDMGFALKYGALYFKVPETIFA
                     EVSGKFPKRVGAKDLILSIAADIGADGATYQAIQFAGKTISKLDMAGRMTCCNMAIEM
                     GAKAGIVEPDKVTWEYMKGRRSLKPFALASDKDADFIDKRTYDVSAMEPQVAVPHNVD
                     TGVPVSTVAGIPVDQVFIGSCTNGRFEDLEEAAAVVGKKKFSSNVRVIIIPASRDEYL
                     KALRAGFIEKFVKAGALVEAPCCGPCMGGAFGLLAPGEVSLSTSNRNFKGRQGSTEGK
                     VYLCSPSTAAASAITGVITDPREV"
     gene            5387..5884
                     /locus_tag="VFC43_01120"
     CDS             5387..5884
                     /locus_tag="VFC43_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HZK29823.1"
                     /translation="MRVWKFSDNIDTDAIIPGRFLTMYDPKELAKHAFEGTRDEYAKK
                     VKPGDIIVGGKNFGCGSSREHAPLALLGGGIKVIVAQSFARIFFRNSINTGVLPIVCK
                     EADRIADGQKITLNIKKGFLEADGRQFPIEPVPEFMQGIIDAGGLVEYAKNLPEVPVC
                     TKSQR"
     gene            5863..6981
                     /locus_tag="VFC43_01125"
     CDS             5863..6981
                     /locus_tag="VFC43_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HZK29824.1"
                     /translation="MYKIAAIGGDGIGPEIVAEGKKVLEAAGERFNFAIDWTDFEIGA
                     DRYLSSGELLTEDDTKELSKFRAIYFGAIGDERVQPGILEKGILLALRFHFDLYVNLR
                     PIRLLKGVETPLAGKKPEDIDFVVVRENTEDFYVGIGSRFRKHEKKVLNVARELYQVK
                     FGLDIESDAEEIAYQIGVVTREGSRRVQTYAFDLAQKRKKKLTSVDKANVLSDVYGLW
                     RDVFSETARKYPDVTTEFTFVDAITMWFVKNPEWFDVVVTPNMFGDIITDLGAMIQGG
                     LGLAPGGNINPKGTSMFEPIHGSAPKYRNLDVANPIATIWAGSLLLDHIGEHEAAAAV
                     VTAIEQSIKDGVVTKDMGGVAGTAKAGSHIANCVKEGK"
     gene            complement(7012..7971)
                     /locus_tag="VFC43_01130"
     CDS             complement(7012..7971)
                     /locus_tag="VFC43_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZK29825.1"
                     /translation="MKGGAQTAESESFYRDLVESQQDLIVKFSPEGRLLFVNSSYCDF
                     VGKSREELVGSVFMPVSAERYSDVVATQMTKLFRPPFTCIVEQWIHAARGLRCISWSA
                     KSVLGTENQVLEIVATGRDITRFKQEQKAIKKKDDELMFLIENGNQMYYSHTPDHVLM
                     FVSPRIRRMLGCRPHEGKRVWTDYLTDNPLNAEGLERTIKAITSGRREPPYRLEMAGK
                     NGKAVWVEVNEIPVVKNQKTVSIVGSIVDVTEKKLVEEALAEAEDLFKDFNGTKKRRY
                     NPPPAEHGKGPMKYIRSILSSQEDEEEDDFPELPHDLKKLIRE"
     gene            complement(8094..8510)
                     /locus_tag="VFC43_01135"
     CDS             complement(8094..8510)
                     /locus_tag="VFC43_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29826.1"
                     /translation="MMEKAGYLQRTGYKAFDSDGNFHDVIEFHILGIRYAIRRADLVK
                     SVAGHLFVQVEEIIHNYNYYLGTTKGLAQVSASGKALNIDIFNEGSYTVSLNTLRNVL
                     HGRERQACIVKIPEQVLPSLKMRRISTDQKRISAMV"
     gene            complement(8639..9766)
                     /locus_tag="VFC43_01140"
     CDS             complement(8639..9766)
                     /locus_tag="VFC43_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HZK29827.1"
                     /translation="MRECENPLWPCAMAGAAACCAGFEGLTVVIHGSSGCYYYPATLL
                     HAPLHGTFIMENEVIFGTEQRLREVLDGLSGAGNKIAVVLTCVPSAMGEDIRSILSDY
                     DVILVDSPGFSGDVEAGYHHALSILAPSVDPDREGINIDGACLFDPFFKGNIQEIVRL
                     LGNASVPAGTVFCSDRLESVSHATPYTIGTNGDFSSGVGTVLGGTLGFDEIRTTFNAI
                     ASVYPDADCGPVEEEMEREEERIVRACDKFLRRFDPPHAVIFSGFSYAVFAAKTLRHY
                     LDAEILFVGSRNEPGACPYLCEQVQGLSEVRNIIRQYNPDLVIGSSFERSVNPDLAFI
                     GLTPPVRGVVKLAPRPVAGITGTLLFVEDVLNTCRDKKKRS"
     gene            complement(9759..10799)
                     /locus_tag="VFC43_01145"
     CDS             complement(9759..10799)
                     /locus_tag="VFC43_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HZK29828.1"
                     /translation="MTLNPSPLNISRFAGCTLTGALSVTTQIRDAVTVIHGPKGCTHH
                     NFSLLHATGLENDRVILPDLISTSLTESDIIYGGEDTLDRTLEEVANRPVSTIFVLST
                     CIVDTIGDDVAAVCRKDRTVQVIPIPTAGFLGGTFQNGVNNALNAIAETARPCKKVRC
                     VNIIGEKNLEYEVDENFAEVLRLLTLLKIPVNLRFLHNCTMNDIVRLGAARLNILRDD
                     DLRSVGDYLEKQFKTPYISSYPFGLAGTIYFLEAVAGIFGVPCSAAVHAEKALQEKIL
                     GAFTDIRGSSITFEPSLIPSESFKIASDVAERIDITIDSGGCRVPLELSPPVGTTGIQ
                     RMLHRWRRAIHA"
     gene            complement(10765..11577)
                     /gene="cfbC"
                     /locus_tag="VFC43_01150"
     CDS             complement(10765..11577)
                     /gene="cfbC"
                     /locus_tag="VFC43_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide reductive
                     cyclase ATP-dependent reductase subunit"
                     /protein_id="HZK29829.1"
                     /translation="MKQIALYGKGGIGKSTTAANLSAALSLQGLDIMQIGCDPKRDST
                     RMLMQGTFIPTVMDLVRVQGEAALSLSDIVFTGFNGVRCVEAGGPEPGVGCAGRGIIA
                     TFQLLEKFGALSGDVIVYDVLGDVVCGGFAMPMREGYAQDIYLVTSGELMSLYAANNI
                     CKAIKRLSSRTKSTCRLAGVICNAKNQPREEDLVADFARRVNSSLIQFIPRDRVVQLA
                     ELNRKTVLEYDPGSSQAAQYRSLADRIMKNTRFTIPTPLEIDDLESLAIEYI"
     gene            complement(11663..13120)
                     /locus_tag="VFC43_01155"
     CDS             complement(11663..13120)
                     /locus_tag="VFC43_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HZK29830.1"
                     /translation="MHRLEHLSTIIADLGDIFVFITPVTAVPVIVAVLFSEWNMLLPT
                     LSVPVTFFLLGTLFKKLPRHNRGVKLSTAMCSVALFWLTCAIVSGFPFMLGLHMGFTD
                     AFFEGMAGWTGTAFSMIRSVDTAPHALLFWRSFMQWIGGIGIIAFMIALGSSTGLISS
                     KIFRTEGRDEPLMPSVIATGRALWKIYGMLTFFAISLILFTGLSLWESVNLAFAAVST
                     GGFTLHEGGILFYRSTALELILIPIMIAGALPFKLYYQIIENRRWRLFEDEQVRLFFL
                     FVVIGVAVVTYDLVFFGNLELFNALQQGLFMIVSSLTSTGFQNANLHSWASVTLFFLT
                     LLTFIGGASGSAAGGIKLHRVALAIRTLLWWFRRFFVSGKVLLPFKIEGRVIPRATAE
                     LEAAKNMLVIILSVVAVFIATLAVVQFHLTTYSVSDIVFVVVSAFSTCGMNAGYVTPD
                     MPIISKWILIGMMWVGRLEVIPVVMLFIAVFKGTD"
     gene            complement(13202..13582)
                     /locus_tag="VFC43_01160"
     CDS             complement(13202..13582)
                     /locus_tag="VFC43_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HZK29831.1"
                     /translation="MTKMFEVYTCSVCGNITKVVHASGGLLVCCGKPMILQQEKTSDA
                     GKEKHVPVIEKSQTGIMVKVGSVPHPMEEKHFIEWVEVRNGENVYVRGFKPGEKPESE
                     FCIPTTDVKVRAYCNVHGLWTNKV"
     gene            complement(13600..14784)
                     /locus_tag="VFC43_01165"
     CDS             complement(13600..14784)
                     /locus_tag="VFC43_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HZK29832.1"
                     /translation="MVSREIIHGVHWVGAIDFDRRLFDELIPLPNGTSYNSYLVQGSE
                     KTALIDTVDPTKEYELISNLVKMNVDRIDYVVVNHAEQDHSGSLPMILEFFPDARVVT
                     NEKCKDLLIAHHHLSPDRFMVIKDKETLSLGDRTLEFFFTPWTHWPETQVTYLREDKI
                     LFSCDLFGSHTATSSLYVTDEAEIYRSAKRYYAEIMMPFRNSIKGYVEMIRALPLVMI
                     APSHGAIYSRPPFILDAYADWVSDAVKNEVVIAYVSMHGSTQKMVDHLTDALITRGIT
                     VKPFNLTRTDIGELAMSLVDTATVIIATPTVLFGPHPQVVYATYLANMLKPKVRFASV
                     IGSYGWGGKAVDTIVKMLDHVKVEVIEPVMVKGLPDETTFLALNLLADEILKKHKSSN
                     IV"
     gene            complement(14797..14958)
                     /locus_tag="VFC43_01170"
     CDS             complement(14797..14958)
                     /locus_tag="VFC43_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HZK29833.1"
                     /translation="MLEKYICCICGHIYDPSKGEPRQDIPPGIAFSDLPDDWQCPICG
                     AERKNFKKE"
     gene            complement(15045..15596)
                     /locus_tag="VFC43_01175"
     CDS             complement(15045..15596)
                     /locus_tag="VFC43_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29834.1"
                     /translation="MKTRLFILVEGEDDVRFFGRIIKPLLIGRYDAVEIIPYASIKRS
                     KVSNFLKSITLMKNDYILISDIDAERSVKDKKQVLYSHFNHIDGGSIIVVIREIESWY
                     YAGLSHESAMALDVPDLSSTDELTKEDFNQLIPNKYDSRIDFMFEILKFFSIVSAGKK
                     NHSFRFFADRYHLEDIETGLLAP"
     gene            complement(15599..>16808)
                     /locus_tag="VFC43_01180"
     CDS             complement(15599..>16808)
                     /locus_tag="VFC43_01180"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZK29835.1"
                     /translation="DLSRFNVVIGSNAAGKSNFISIFKFLRDIAKHGIVNAIAMQGGS
                     EYLQNARIGSSHDLYIRVVYHPDQKTEFLVRQESDLPSLAVQSCQSSYEFAIRFAPKG
                     DGFTIIKDSLVLGCEVMSCKHKNNHSIEKESIGHGEIRVTNEGGAVTYTADLPEGCPL
                     SEEEIIPLCFRKKHLPEKTLLLETPYAFPLSHGEKFFNRIAVYDIDPRLMKKGVAITG
                     KRELEEDAGNLALVLKNIVGDSEKKRKFSNLLRDVLPFIEDFSVEKFMDISLILTLRE
                     KYAKNLNLPSPSISDGTLTIFALIIVLYFEDKPFIIIEEPVSHIHPFLVARVISMMKE
                     SSKRKQVMITTHTTEVVKHATLDHLLLISRDLEGFSVISRPADKEEVRTFLENEIGIE
                     ELYVQNLLGM"
BASE COUNT         4208 a         4147 c         4176 g         4277 t
ORIGIN      
        1 cgggcgggat ctgctcaggt agtgagatct gtggatttcg gtccgcccct tcacatactg
       61 gtagtccccg cagaactgca tgagatggaa cggacgtacc tggagatgtt tgccggttta
      121 tgaagattgc agaatgccag gtcctgttga cccggacgct ctcagcaacg tacgtatctg
      181 ctccgaaggg aactcatttc taccgcattg ctgctcaaat cctggaaatg gcacgtgcgt
      241 atgaatccga tggcatcagg tttttccaga ccggcgatcc cgtgaacgca ctggcgagtt
      301 attactatgg gttcggctgg ctgcacttcg gcgtgtcatc cggcctgctg aatatcccct
      361 gtcctgccgc ttgcccgttt aaagggcctg ctgaagtact tcacatccgg cacaggtcaa
      421 agctcgagga gaaaacaacc cgctatgcac gccttctgga catagccagg tcttctgtga
      481 agtgttcaat ggacccggcg acaaccagca gtgtatttgc ccggaggatg ctgtgtatcg
      541 ccggtaccta tgccaggcgc ggggagtatc ttgtgaatgc aggtgaccct gaaggcgctc
      601 ttgccagttt cagttacggg catggctggc tcgatgctgc cgtaacggca ggattcttct
      661 gtatcgtcgc agaacgagac cttttcacca tctgatccag acaaagaaac ctttgggata
      721 ctccgtggga gtgaaacccc cggattcaag tcataaaagc gtttatacta tgttcaataa
      781 tttgtaagat acaacgcaca tttttttagt gtgccattct ttcggggaac tcacatggaa
      841 aaatcgcagg taaaatggct ctacatttct gtcgggttct cccttctcgt tttagtaata
      901 atcctgtact tcaccatcaa tgagaacacg gtttactacc tcagacagat caacccggtc
      961 ttcctttttc ttgcattcat gacccacatc cttaccatgt gcttctgggc attgcgcatc
     1021 aagaaaatgt ccggctcgct gggttactgt ataggctttt tttacagcct gaaccttgtt
     1081 tttgcaaacc ttcttgcagc cgcaataact ccggcccagg caggaggaga gcctgtccgg
     1141 gttcacgagc tctaccgggc gaatgtgcca atcggggatg caacggcaat cgtgatcatg
     1201 gaaagagctc tggatggtat tgtacttgcc gcactcgcgg cctttgcgat gattgtcctg
     1261 acagaccagt gggagagtct tggagcgatc tcggaaatca tggtgtatat tacctgggtc
     1321 tttgttgcag gatgcctgat cctcttttac cttgcagtaa aacggccgga tgttgtgaag
     1381 agggttgtaa accgtattgc ctgcttcttc acccgcacat gggaaaatgc aagggttgaa
     1441 aaatttatcc tgcgggctga caaagagatc gacaatttta atgatgcggt gataaaattt
     1501 gtgagaagtg caaagggtgg tttgttgtgg gggttggttt ttaccctgct ctactgggta
     1561 tcagagattc tcaccgcttc acttatcctt gtcgggctcg ggcagccgcc gctgatcctt
     1621 gaatcatttg ttatccagct catcctggca atcctgatga tgctcccgct cacacccgga
     1681 agctccggta ttgccgaagc aggagcatat tcaatgtacg ccttgttcat ccctgtttcg
     1741 attgtgggga tattcgttgt tatctggaga attgttctct actacttcaa tattgcactt
     1801 ggcatcctct caagtattat tatcgtccgt cgtgaagctc aatgcaagga ataatcctgt
     1861 agcgctgacc ttaactctta tcatggtgaa tgacagcaat caataggaaa acccatgact
     1921 caggtcagat gtgaggttaa gggggtattt gtcgcgatga gtgatgcaac tacaatacct
     1981 gttgtactgc tcactgatgg cggacagctg agcctgccga tatttatcgg gatctgggaa
     2041 gcggtatcta tcaacagtgc aaaaaacaag gaaattctct cccgcccgtt cacccatgac
     2101 ctctttttag accttgcggc aaaattttct atcacgtttc actcattgca gatagacagc
     2161 attgaagacg gggtctatta tgcccagctc gtgctctcgg ccgatcacca cagggagttt
     2221 attgactgcc ggcccagtga cgggatcgcc cttgccctca gggcagatat ccctattttt
     2281 gttgataaag acgtgcttga atccgcaggc aagaaatttt cagatctccc ccctcttgtg
     2341 gacttgtcaa catttctgcg gaaatagcgc tattttcgcc gcttttgtaa ttcctgcata
     2401 acgtcagaga gatcgattcc ggctgcacaa agagcaacaa gaaggtggaa aagaaggtca
     2461 gatgcttctt ctgttgtgcg ttttttgatc ccgtttttgg cggcaaggat aaattctatt
     2521 gattcttctc ctaccttttc aagcaccttg tcgatgccct tgggatccgt gagcaaccgg
     2581 ctcgtgtagg attcagccga aggattgttt gcccgttcct ggatgactga ccagatttca
     2641 gaaattattg ccggatcaac attgtctttc atgcatcctc ctggataagg aggacttccc
     2701 caacatcgga cccaaagaac ctccgtgaaa ggatgcgcgc cttttcaata ttgcatcccg
     2761 atttcccgat agcaataccg aggtcactcc tctgcttcac aaagacattc aatggcccgt
     2821 tttcggcacc ccgctcaatc ctcaccactt cggcaggttt gaagatgttc atgatgaatt
     2881 cctcaggggt ttctgcatat tccaccatct caatgcgctt acccagcaca ctctggagac
     2941 gtctgatatt atcccctttt ttcccgatgg ccagtcccat atctccctgg cgtatcacgt
     3001 agatcacccg gtcaaaacga tcatctatga cgcagtcaag tgcagtggat tttgtgagaa
     3061 ttcttaattc ctcaatatac cttcgctctt taaagccgat attgcgttcc atatcaaaat
     3121 caatcctatt gtatccgcga tttttggata ttcgaaaatt ctcttgcgtg ggataatttt
     3181 gtccggcaca gctggaccat acattaggtt taaggaataa tcatacactg ctactgtaaa
     3241 aaagttgtga agagatcctg acttagatca gcggatcgta taggtaatgg aaacctgtgc
     3301 attcaccgtc acatcaccgg gttgtatcgg agtgggggtt gttggtgctg aaacagcgtt
     3361 gtcgaccttg taattttcaa agaggaccgg ggaataaccg ccgctgatat cgacgctctg
     3421 cacacccaat attgtcgtac ccagcgcacc ggcgacagta tcagcatctg cgcgtgcacg
     3481 tgatactgcc ttttttaagg cctcggtgcg gagggtctgc gactgttcat cggaaagcat
     3541 gaactggatg gaactggcct ggtttatacc attttttacc gcaatatcga tcacttcgcc
     3601 cgtccggttg acatcatgga gcgtaaccgt cagggtattg gtgacctggt atgtacggat
     3661 tttctgctca aacggagact ttgagtaatc atcatacaag gggtaaatgt tatacccggt
     3721 tgtcttgagt gcgtcttcag gcaggccggc ggcaccgagt gcatcaatta cctttttcat
     3781 ctgcaccgca tttgcctgct gggcagttct cacatccgga ttttcggtca cgactgaaaa
     3841 ggtgacctgg gcccggtcag gagttccaat cacattgccg cttccggttg catggatcac
     3901 gtttaccgcc gcggtgtctg ccggtgctgc gagcacactt actatacagg tcatcgcaac
     3961 gataacgatg gcgaccgtaa ggagagagaa ttttttcatc ataattatca gttttacttg
     4021 tctggaatgt agtttaaaga ttgctttgac tgcgaaaata cctgcagtta tgccggaaat
     4081 ttccctcccc caaactacaa tattattgag cacctatagt accccgataa cgcatgggag
     4141 cgacaatcgt agagaaaata ttttcccgga aatgtggcag ggatatacga gcaggcgaag
     4201 tagtcatgac gccactcgat ggtgcgatga ttcatgatat taccggcccc cttgcgatcc
     4261 agaagttcta cgaaatgggg ggaacgaagg tttttgatcc ccggcgcgtc atcatgctct
     4321 ttgatcacca gataccggca gattcgatcg ctgcagcaga gaaccacgtc tacatgcgga
     4381 gatttgccgt agaacaacag atccataatt atgatatccg ggaaggtgtc tgtcaccagg
     4441 ttgttgttga gaaaggaaga gcagcccccg gtgagatcgt tgttggtgcc gattcccata
     4501 cctgcatgta tggtgcggcc ggtgcgtttg ctaccggcgt cggttcaacc gacatgggat
     4561 ttgccctgaa gtacggtgcc ctgtatttta aagtccccga aacgattttc gcggaagtct
     4621 caggaaaatt tccaaagcgt gtcggggcaa aggatctcat cctttccatt gccgcagaca
     4681 ttggtgccga cggggcaacg taccaggcaa tccagtttgc cggaaaaaca atttcaaaac
     4741 tcgatatggc aggacggatg acctgctgca atatggctat tgaaatgggc gcaaaggccg
     4801 gcatagttga gccggataaa gttacctggg aatacatgaa agggcgtcgt tctttaaagc
     4861 cgttcgcgct cgcgagcgac aaggatgccg actttattga caaacgtacc tatgatgtct
     4921 cggcaatgga accacaggtc gctgtgccgc acaatgtgga taccggcgtg ccggtcagca
     4981 cggttgccgg tatacccgtt gaccaggtat ttatcgggtc atgcacgaac ggacggtttg
     5041 aggaccttga ggaagcagca gctgtcgttg gcaaaaagaa gttcagcagc aatgtccggg
     5101 tcatcattat cccggcttca cgggacgagt acctgaaagc cctgcgtgcg ggtttcatcg
     5161 agaaattcgt aaaagctggt gcactcgtgg aagcaccatg ctgtggtccc tgcatgggag
     5221 gggcgttcgg gctgctggca ccaggagaag tatccctgtc tacgtcgaac cggaatttca
     5281 agggccgcca ggggagcact gaggggaaag tctacctctg ctcaccatct acagcagcag
     5341 caagtgcgat taccggagtt ataactgatc cgcgggaggt ctgaccatga gagtctggaa
     5401 gtttagcgat aacattgata ctgatgccat tattccgggg aggttcctga ccatgtatga
     5461 cccaaaggaa ctcgcaaagc acgcgtttga agggacccgg gacgagtatg ccaaaaaagt
     5521 gaaacccggg gatattatcg tcggagggaa aaatttcggg tgcggctctt cccgggaaca
     5581 tgccccgctc gccctcctcg ggggaggcat aaaggtaatt gttgcacagt catttgcccg
     5641 gattttcttc cggaattcga tcaacaccgg cgtgcttccc atagtctgca aagaggcaga
     5701 caggattgct gatggccaga agatcaccct gaatatcaaa aaaggcttcc tcgaggcaga
     5761 tggcaggcaa tttcccatcg aaccggttcc ggagttcatg cagggtatca tcgatgccgg
     5821 aggactcgtg gaatacgcaa agaatttacc tgaggtgccg gtatgtacaa aatcgcagcg
     5881 ataggcggag acgggattgg cccggagatt gtggccgagg gaaagaaagt actggaggct
     5941 gcaggagaac gcttcaactt cgcaatagac tggacagatt tcgaaatcgg ggctgaccgc
     6001 tacttatcca gcggggaact cctgaccgaa gacgacacaa aggaactctc gaagttcagg
     6061 gcaatctatt ttggtgcgat aggggatgaa cgggtgcaac ccgggatact ggaaaagggt
     6121 atccttctgg cgctccggtt tcattttgac ctttacgtta acctgcgtcc gatccggctc
     6181 ctcaaaggtg tagaaacccc gcttgccggg aaaaaaccag aagacatcga ctttgtcgtg
     6241 gtcagggaga acactgagga cttctacgtt gggataggtt cacgattccg gaagcacgag
     6301 aaaaaagtgc tgaatgtagc ccgggaactc taccaggtga agtttggcct tgatatcgag
     6361 agtgatgccg aagagattgc gtaccagatc ggtgtcgtca cgcgggaagg atcccgccgc
     6421 gtccagacct atgcttttga ccttgctcag aaaaggaaaa agaaactcac ttcggtggac
     6481 aaggcaaacg tgctctcaga tgtgtacggg ttatggagag atgtcttttc agaaacggcc
     6541 aggaagtatc ctgatgttac taccgagttt acgtttgtcg atgccatcac catgtggttt
     6601 gtaaaaaatc ccgagtggtt tgatgtggtg gttactccta atatgtttgg cgatatcatc
     6661 accgatctcg gtgcgatgat ccagggggga ctcgggcttg cacccggggg aaacatcaac
     6721 ccgaagggca catcgatgtt tgagcccatt catggctcag cacccaaata caggaacctt
     6781 gacgtggcaa accccatagc aacaatctgg gcaggatcgc tgctgctcga tcatatcggg
     6841 gagcacgagg ctgctgcggc agttgttact gctattgaac agagtatcaa agacggtgtt
     6901 gtaacaaagg atatgggggg agtggccggg acggcaaagg cagggtcgca tattgccaat
     6961 tgcgtaaagg aagggaaata ggatttttta ataccccgga acctgcgaaa tttactcccg
     7021 tataagtttt tttaagtcat ggggaagttc gggaaaatca tcttcttctt catcttcctg
     7081 agaagagagg atagaacgga tgtatttcat cggccctttc ccgtgttcag ctggcggggg
     7141 gttgtacctg cgttttttag tgccgttaaa atccttgaag agatcttcgg cttctgccag
     7201 tgcttcttcc accagctttt tctcggttac atccacaata gagccgacaa tcgagaccgt
     7261 cttttggttc tttactacgg gaatctcgtt gacttcaacc cagacagcct tcccgttctt
     7321 tccggccatc tcgagacggt acggaggttc acgtcttcct gaagttatcg cttttatcgt
     7381 tcgttcgaga ccttcggcat tcaggggatt atcggtaaga taatcagtcc atacccgttt
     7441 tccttcatgc gggcggcatc ccagcatccg gcgtatcctg gggctgacaa acatcaatac
     7501 atggtcagga gtatgggaat agtacatctg gttcccgttc tcaatcaaga acatcagctc
     7561 gtcatctttc ttttttatcg ctttctgttc ctgtttaaac cgggtgatat cacgaccggt
     7621 tgctacaatt tccagtacct ggttctcagt tcccagcacc gactttgctg accagctgat
     7681 acatcgcaat ccccgcgctg catgtatcca ctgttcaaca atacaggtaa aaggcgggcg
     7741 gaacagcttg gtcatctgcg tggcgacgac gtcagaatac cgctctgccg ataccggcat
     7801 gaaaacgctg ccaactaatt cctcccggga ttttccaaca aaatcgcagt acgaagaatt
     7861 aacaaaaaga agacgaccct ccggactgaa tttaacaata agatcctgct gggactcgac
     7921 aaggtcccgg taaaaactct ccgattctgc cgtttgtgct ccccccttca taggtaattg
     7981 aaatgacatc cctggttttt taaaaaaaag agatgccttc tttcctcagg atcatctgtc
     8041 gaaggactat taatgttgta cgatatgcac agattctgtc agatctgcaa cagctatacc
     8101 attgcagata ttctcttctg gtcggtggag attcttcgca tctttaacga tggaaggacc
     8161 tgctcgggta tcttcacgat gcatgcctgc cgttctctgc catggaggac attccggagt
     8221 gtgtttaagg agaccgtgta gcttccctca ttgaatatat caatattcag ggctttaccg
     8281 gacgctgaca cctgggcgag tcctttggtc gtcccgagat aataattata gttatggatt
     8341 atttcctcga cctgaacaaa caggtgacca gccacagatt ttacgagatc agcacgccgg
     8401 atagcgtacc ggattcccag gatgtgaaat tcgatgacat cgtgaaaatt cccgtcgctg
     8461 tcaaatgcct tgtacccggt ccgctgtaga tatcctgctt tttccatcat acacgaccac
     8521 ctttcagtcg tactacggcg ttgggagtat cggtatataa cccattgagt ctgcgcggtg
     8581 tgaaagtgat taaaagagca gtctgacgaa atcgtttccg gtccgggtat gcaccaattc
     8641 acgatctctt ctttttgtcc ctgcaggtat tgagtacgtc ttcgacaaac agcagcgtgc
     8701 cggtaattcc tgcaacaggg cgcggggcca gcttgaccac tcctctgacc gggggggtaa
     8761 gcccgataaa agcaaggtcc gggttcacgg aacgttcaaa cgaagacccg ataacgagat
     8821 ccgggttgta ctgccggata atgttcctga cttctgacaa cccctgcacc tgttcacaca
     8881 ggtaggggca tgccccgggt tcgttgcggg aaccaacaaa caggatttcc gcgtcaaggt
     8941 aatgccggag ggtttttgct gcaaataccg catacgaaaa accactgaat attactgcat
     9001 ggggcgggtc gaacctgcgg agaaatttat cacaggcccg gactattctt tcttcctccc
     9061 tttccatctc ctcctcaaca gggccgcaat ccgcatccgg gtacaccgag gcaatggcgt
     9121 tgaatgttgt ccgtatctca tcaaaaccca aagttccccc cagcactgtc ccgacaccgc
     9181 tggaaaaatc cccgttggtc cctatagtgt agggagttgc gtggctcacg ctttcaagcc
     9241 ggtccgagca aaaaacagtt ccggcaggca ctgatgcatt acccagcaat cgcacaattt
     9301 cctggatatt acctttaaaa aaaggatcga aaagacaggc cccgtcaata tttatccctt
     9361 ctctgtccgg atccaccgag ggagcaagaa tggagagagc atggtggtat cccgcttcaa
     9421 catcaccgga aaaccccggg ctgtcgacca ggatcacgtc atagtcactg agtatggatc
     9481 gtatatcctc ccccattgcc gaggggacgc acgtcagaac tacggcaatt ttgttaccag
     9541 caccagaaag cccgtccagg acttcacgaa gtcgctgctc tgtcccgaaa atcacttcgt
     9601 tttccatgat aaaagtgcca tggagaggtg catggagaag agtggcagga taatagtaac
     9661 aaccgcttga accgtggatc acaacagtca gaccctcaaa accggcacaa caggctgcag
     9721 ccccggccat cgcacaaggc cagaggggat tttcgcattc acgcatggat tgcccgcctc
     9781 cagcggtgca gcatcctctg gatccctgtt gttcccacgg gtggtgacaa ctcaagggga
     9841 accctgcacc cgccgctgtc tatcgtaata tcgatccgtt ctgcaacatc agaagcaatc
     9901 ttaaatgatt cagaagggat cagggaaggt tcgaacgtta ttgaactgcc cctgatatcc
     9961 gtaaatgcac cgaggatctt ttcctgcagg gctttttccg catgcacagc tgctgaacag
    10021 ggtaccccaa aaatccctgc aacagcctcg aggaagtaaa ttgttcccgc aagaccaaag
    10081 ggatatgacg agatataggg tgttttaaac tgtttttcca ggtaatcacc aacagatctc
    10141 aggtcgtcat cccgaagaat attaagccgt gctgccccga gacgtacaat atcgttcatg
    10201 gtgcagttgt gtaagaaccg caggtttacg ggtattttga ggagtgtcag taaccgtaaa
    10261 acttcggcaa agttttcatc aacttcgtac tcgaggtttt tttcaccgat aatattgacg
    10321 cacctgactt ttttacaggg ccgggcagtt tcagcaatgg cattgagtgc attattcacc
    10381 ccgttctgga atgttccccc aagaaatccc gctgtcggta tggggataac ctgaacggtg
    10441 cgatccttcc ggcagactgc ggcaacatca tcgccaatgg tgtccacgat acaggtggaa
    10501 agcacaaaaa tggtgctgac cggccgatta gctacctctt caagggtccg gtcaagagtg
    10561 tcctctcccc cgtaaataat atcactctcc gtaagactgg tcgagatcag atccgggagt
    10621 ataacgcggt cgttttccag tccggttgca tgcaggagtg aaaagttgtg atgggtgcac
    10681 ccctttggtc cgtgaattac ggttacagca tccctgatct gggtagtcac cgacagtgct
    10741 ccggtgagcg tgcaccccgc gaacctagat atattcaatg gcgagggatt caaggtcatc
    10801 aatttcaagg ggtgtaggaa tggtaaaccg ggtgtttttc ataatacgat cggcaaggga
    10861 cctgtactgt gctgcctgcg atgagcccgg atcatattca agcacggttt tccggttcag
    10921 ttctgcaagc tggacaacac ggtcacgcgg gatgaactgg ataagactgc tgttcacccg
    10981 acgggcaaaa tccgcaacaa gatcttcttc gcggggctgg ttcttggcat tacagattac
    11041 cccggcaaga cggcacgtgc tctttgtccg cgatgataag cgttttattg ccttgcagat
    11101 attattcgct gcgtacaggg acatcagttc gcctgatgtc acaaggtaga tatcctgtgc
    11161 atacccttcc cgcatgggca tggcaaatcc gccgcatacc acatccccaa ggacgtcata
    11221 cacaataaca tcgccgctga gggctccaaa cttctcgagc agctggaacg tggcaataat
    11281 ccctctgccg gcacatccca cacctggttc cggcccgcct gcttctacac accgtacacc
    11341 attaaatcct gtaaatacga tatcggaaag ggagagtgca gcctctccct gcacccggac
    11401 aaggtccatt acggtaggaa taaaagtccc ctgcatcagc atccgtgtgc tgtcccgttt
    11461 gggatcacat ccaatctgca taatatcaag gccctgaagg gaaagggctg cggagaggtt
    11521 agcagccgtg gttgatttcc cgataccgcc cttgccatac agggctatct gtttcatcac
    11581 catactatgg ggtggcaatt tatattagat ctcctgatgt taagaaaagt aaagttgtct
    11641 aacagcagtt gcaggaaaaa atttagtccg ttcccttgaa cacggcaata aagagcatca
    11701 ctaccgggat cacttccagc cttcccaccc acatcatacc gattaagatc cacttggaaa
    11761 tgatcggcat atccggggta acataacccg cattcatccc gcacgtggag aaggctgata
    11821 ccacgacaaa gacgatatcg ctgactgagt acgtggtgag gtggaactgc actaccgcga
    11881 gagtagcgat aaaaacagcg accacggaga ggatgataac aagcatgttc tttgcagctt
    11941 cgagttcagc ggttgcccgg ggaatgaccc tgccttcgat cttgaacggc aggagtacct
    12001 taccgctgac aaagaacctt ctgaaccacc agagcagtgt ccggatggca agtgccaccc
    12061 ggtggagttt gattcccccg gcggccgacc cggatgctcc cccgatgaac gtcagcaggg
    12121 tcaagaaaaa caacgttaca ctcgcccagg aatgcaggtt ggcattctga aaacccgtgc
    12181 tggtgaggct cgaaacgatc ataaatagac cctgttgcaa ggcgttgaac aactccaggt
    12241 tcccgaaaaa gacgagatca taggttacta ctgctactcc gataaccaca aagagaaaaa
    12301 acagcctgac ctgttcatcc tcaaagagcc tccatcgccg gttttctatg atctggtaat
    12361 agagtttgaa cgggagagca ccggctatca taatcgggat gaggatgagt tccagtgcag
    12421 tgctccgata gaagagtata cccccttcgt gcagggtaaa gcctccggtc gataccgccg
    12481 caaacgcaag gttgaccgat tcccataatg agagcccggt gaacaggatg agactgatgg
    12541 caaaaaaggt taacatcccg taaatcttcc aaagggctct tcccgttgcg ataacactgg
    12601 gcatcagtgg ttcatctctt ccctcggtgc ggaagatctt tgaacttata agaccggtgc
    12661 tgctcccaag cgcaatcata aaggcaataa ttcctatgcc tccgatccac tgcatgaagg
    12721 atctccagaa aagaagggcg tggggggcag tatcaaccga gcgaatcatg gaaaacgcag
    12781 tccctgtcca tcctgccata ccttcaaaga acgcatcggt aaatcccata tgcagaccca
    12841 gcatgaaggg aaaaccgctg acaatcgcac acgttaacca gaaaagtgcc acggaacaca
    12901 ttgcggtgga caactttact cccctgttgt gccggggtaa ttttttgaaa agtgttccaa
    12961 ggaggaagaa ggtgacgggg actgacaggg taggcaggag catattccac tcggagaaca
    13021 gtaccgcaac gatgacggga accgcggtga caggcgtgat aaatacgaaa atatccccga
    13081 gatctgcaat aatcgtggaa agatgctcaa gccggtgcat cactaatggg ttaccgcttg
    13141 ggctctatta gtgtttgcct gagtaaacga gaaaaaaggg gatttttatg tgtggtcttt
    13201 tttatacttt atttgtccat agtccgtgaa cattgcagta tgcacggact ttgacatcag
    13261 ttgtgggaat acagaattca gattcaggtt tttccccggg tttaaaaccc ctgacgtaga
    13321 cattttcccc gtttcttacc tcgacccact cgataaaatg cttctcttcc atcgggtggg
    13381 gaacggaccc gaccttaacc atgattcccg tttgagattt ctcaataacg gggacgtgtt
    13441 tttccttccc tgcgtcagaa gtcttttcct gttgcaggat catcggtttc ccgcaacaga
    13501 caagcaggcc acctgatgca tggaccacct tggtgatatt tccgcacacg ctgcatgtat
    13561 atacttcaaa cattttcgtc atatcagaag tcctccttcc tacacgatgt tactactctt
    13621 gtgcttcttt aagatttcgt ctgccagcag gttgagtgcc aggaacgttg tctcgtctgg
    13681 aagacccttc accatgacag gctcgatgac ttcaaccttg acatggtcaa gcatttttac
    13741 aattgtatcg acagccttcc cgccccagcc gtacgatcct atcacagagg caaaccgtac
    13801 ttttggtttg agcatgtttg caagatacgt ggcatacacg acctgcgggt ggggaccgaa
    13861 aagtactgtc ggggtggcaa taataacggt tgcagtatcc acaagggaca ttgcaagctc
    13921 accgatatca gttcgggtaa ggttgaaggg ttttaccgta attccgcggg taatcagtgc
    13981 atcggtcagg tgatccacca ttttctgcgt gcttccatgc attgatacgt acgcgatgac
    14041 tacttcgttt ttcacggcat cagataccca gtcggcatag gcatcgagga tgaaaggggg
    14101 acgggagtag atggccccgt ggctgggagc aatcatcacg agaggaagtg cccgtatcat
    14161 ctcaacatac cctttgatac tgttgcggaa cggcatcatg atctcggcat agtacctctt
    14221 tgcggagcga tagatttccg cttcatcggt aacatagagg ctgcttgttg ccgtatgaga
    14281 cccgaacagg tcacaggaaa agaggatttt atcctcacgc aggtaggtaa cctgggtctc
    14341 gggccagtgt gtccacgggg tgaagaaaaa ttcgagcgtg cggtctccca aagaaagggt
    14401 ttctttgtcc tttattacca tgaaacggtc tggcgacaga tgatgatggg cgatgagaag
    14461 atccttgcac ttctcgttgg tcactacccg tgcatcggga aaaaattcca gaatcatcgg
    14521 cagtgaaccg gaatggtcct gttcggcgtg atttacgacg acataatcga tccggtcaac
    14581 attcatcttg acaaggtttg aaataagttc atactccttt gtgggatcga ccgtgtcaat
    14641 caatgcggtc ttctcgctgc cctggacaag atacgaattg tagcttgtac cgttcgggag
    14701 cgggatcagc tcgtcaaaga gccttcggtc aaaatcaatt gctccgaccc agtggacgcc
    14761 atgaataatt tctcgtgata ccatagttac cagcgattac tcctttttga aattttttct
    14821 ttctgcaccg catatcggac actgccagtc atcaggcagg tcagaaaatg ctattccggg
    14881 ggggatgtcc tgtcttggtt ccccctttga cgggtcataa atatgcccgc aaatacagca
    14941 gatatatttt tccagcacgt gaaaagcctc cggatatttt aaactacatc agattaaggc
    15001 agagatttga attaatcatt tcgatgctgc ataataggca tccattatgg agccaagagt
    15061 cccgtttcta tatcttccag atggtaccgg tcagcaaaga acctgaatga atggtttttc
    15121 tttcccgccg aaacgatgga gaaaaacttg aggatttcaa acataaaatc gatccgagaa
    15181 tcatacttgt tcgggataag ctggttgaaa tcctcctttg tgagctcgtc ggtggaggag
    15241 aggtcaggaa catcgagggc cattgctgat tcatgcgaga ggccggcata ataccagctc
    15301 tcgatctccc tgatcaccac gataatactt cccccgtcaa tatgattgaa atgggagtag
    15361 aggacctgtt ttttatcctt cacggatcgt tctgcgtcaa tgtctgaaat taaaatgtag
    15421 tcatttttca taagcgtgat gctttttaaa aaattactca cttttgagcg tttgatgctg
    15481 gcatagggaa taatttctac tgcatcgtaa cgcccgatca gcaggggttt gataattcgc
    15541 ccgaaaaacc ggacatcatc ttcaccttcc acgagaatga acaagcgggt tttcatgctc
    15601 acattcccag caggttctgt acgtacagtt cctcgatccc gatctcgttt tcaagaaacg
    15661 tccgcacttc ttccttatca gcggggcggg aaatcacaga gaaaccctcc aaatcccgtg
    15721 atatgagaag aagatgatcc aacgtggcat gtttcactac ctcggttgta tgggtggtga
    15781 tcattacctg ctttcttttt gaggactcct tcatcatgga aataacccgg gcaaccaaaa
    15841 atgggtggat atggctgacg ggttcttcaa tgatgatgaa tggcttgtcc tcaaaataca
    15901 gaacgatgat gagcgcaaag atcgtcaatg taccatctga aatcgatggt gaggggaggt
    15961 tgagattctt tgcatatttt tcacggagtg tgaggataag cgagatatcc ataaattttt
    16021 cgacggagaa atcctcaata aaaggaagga catctctcag gaggtttgaa aatttccgtt
    16081 ttttttccga atccccgacg atatttttta agaccagcgc aaggtttccg gcatcctctt
    16141 caagttccct cttacctgtt attgccacac cttttttcat gagcctggga tcgatgtcat
    16201 agaccgcgat gcgattgaag aatttttctc cgtgagataa aggaaaggca tacggggttt
    16261 ctaaaaggag cgttttttcc ggcaggtgtt tcttacggaa acaaagaggg atgatctctt
    16321 cttctgagag cgggcacccc tccggcagat cagctgtgta ggttacagca ccaccttcat
    16381 tcgtgacccg tatctcgcca tgtccgatgg actccttttc gatactatgg ttgtttttat
    16441 gtttgcacga catcacctcg caaccaagaa cgagactgtc ctttattatg gtaaaaccgt
    16501 cgccctttgg agcaaacctg atagcaaatt cataggatga ctggcatgac tgtaccgcaa
    16561 gggatggtaa gtccgactcc tggcgtacaa gaaactccgt tttttgatcg ggatgataca
    16621 caacccttat gtaaagatcg tgtgaagatc cgatcctggc attctgaagg tattccgacc
    16681 ccccctgcat tgcgatggca ttgacgatgc cgtgtttggc gatatcccgc aggaatttaa
    16741 atatgcttat gaaattggat tttcccgcgg cattggaacc gatcacgaca ttaaaccggg
    16801 ataagtct
//
LOCUS       DASRLV010000030         3316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_575827,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000030 DASRLV010000000
VERSION     DASRLV010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575827"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VFC43_01185"
     CDS             <1..470
                     /locus_tag="VFC43_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29836.1"
                     /translation="SSNSIAQQTGFEQRRVLRALTKIRFVMVKDIPEIFSGTVEVDET
                     YLGVNGKTNEKRSEIRVLSGEEGPRNNRYSESFVAMVLSGQRLSMTLKLVLFNHSSHK
                     KYQRVLSSVLILGKLIPESQHEDMSIVSLITAKNSTAMEKEITSMVWRGSGVT"
     gene            715..1614
                     /locus_tag="VFC43_01190"
     CDS             715..1614
                     /locus_tag="VFC43_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZK29837.1"
                     /translation="MISDEPLDLLTIIKSHIPDQKRPISLVRQDYLAFYEDMQDDRGE
                     YMITERVELSRSVYGYWITVPESESRCTILFFHGGDFTVGSTKDHLGLCIQIARAARA
                     QVFSVDYRLAPEYVFPSAIEDAMAAYRYLIGHGHQPHRIIPVGISAGGTLVIDLLISA
                     RDQGLPLPRAGVCMSPPVDMLFSGESVKRNQDNDWMTLERLDAIRTVYLAGHDPKDPL
                     VSPVFAHLYGLPRLYIQAGTHELLLSDIASFVDKARWAGVPVQFELWEGMFHSWQVFA
                     RDIPEGREAISHIGAYALDVLNR"
     gene            1715..2563
                     /locus_tag="VFC43_01195"
     CDS             1715..2563
                     /locus_tag="VFC43_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HZK29838.1"
                     /translation="MFESIGRSFALVKTSWNILMDDKKLLLFPIISGIVMLFVLLTFI
                     LPLILAGTFMNIPGVGPVFFYGLLFAFYVASYVVGIFFNTALITCVNARFQGRDMAVG
                     EGLSSAARHLPSIFLWALVSATVGVLLHLIQRRAGFIGRFAAALVGGIWSLVMFFVVP
                     VLILEDKGVVDAVKESVALIRRTWGESIVGSGSIMLIFVFIGLFGMVCVFASLLVGNM
                     IVFGSAVVLFIILIVLLAIVASAMQGIFITALYTYARTGIVPSEFNQDQIRNAFIPTQ
                     AGPGTI"
     gene            2751..3182
                     /locus_tag="VFC43_01200"
     CDS             2751..3182
                     /locus_tag="VFC43_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29839.1"
                     /translation="MDTVTAWEALPGKTQDEKLGSLGVTKDRMLDIRMLDEISRTYGI
                     EIFLFFEEDLARTRKLESVLKEFEIFPEFGRPYIRIGSFLRFTRENDPSFEQTLREFP
                     LMVEIVTIGETPQPGQGKPMQYITGLMPFLDELDVDADPVI"
BASE COUNT          819 a          748 c          829 g          920 t
ORIGIN      
        1 aaagttcaaa ttcaatcgcc caacagaccg gatttgagca acgacgggta ctcagagcat
       61 taaccaagat tcgatttgtg atggttaaag atataccaga gatcttctcc ggtactgttg
      121 aagtggatga aacatatctc ggggtcaatg gaaaaacaaa cgaaaaacga tccgagataa
      181 gggtactaag cggggaagag ggaccaagaa acaaccggta ttcggaatcc tttgtcgcaa
      241 tggtactgtc tgggcagagg ttgtcgatga cgttgaagct ggtactcttc aaccactcat
      301 ctcacaaaaa gtatcaacgg gttctatcgt ctgttctgat acttggaaag cttataccgg
      361 aatcgcagca cgaggatatg tccatcgtct cgttaatcac ggcgaaaaac agtacagcga
      421 tggaaaagga aatcacatca atggtctgga ggggttctgg ggttacctga aacgaaaact
      481 tgcgtcgaaa ggaggcattc gtaaagacag gctgcatctc ttcttaggag aatacgtctg
      541 gagatacaat catcggtcag atccagaaag gataaaaatg cgaagactca tttcaactct
      601 aagagaatac taggttttaa attcattccc agggaaaaaa gtaataaagt gtcagttgtg
      661 cccggcgcac cataccctct tcctttatct tcctccttca ccatatccaa attaatgata
      721 agcgatgaac ctttagatct ccttaccatt atcaaatccc atattcctga ccagaagagg
      781 ccaatatcac tcgtacgtca ggattatctg gcattttacg aagatatgca ggatgaccgg
      841 ggagagtaca tgattaccga gcgtgtggaa ctctccagga gtgtctatgg ctactggatt
      901 accgtgccgg aatcagagag cagatgcacg attctttttt tccatggcgg tgattttacg
      961 gtcggatcga caaaagacca cctcggattg tgcatacaga ttgcgcgggc ggcaagagcg
     1021 caggttttct cggttgatta ccggctggca ccggaatatg ttttcccttc agcaattgaa
     1081 gatgcgatgg ctgcgtaccg ctacctgatc ggtcatggtc atcagcccca caggattatc
     1141 ccggtcggta tatctgcggg aggtactctg gtgatcgacc ttctgatatc ggcccgggac
     1201 cagggattac cgctgccccg tgcgggcgta tgcatgtcac cacctgtgga tatgcttttt
     1261 tccggggagt ctgtgaaaag gaaccaggat aacgactgga tgaccttaga aagactcgat
     1321 gcaataagga cggtctacct tgcagggcac gacccgaaag atcccctggt atccccggtt
     1381 tttgcccatc tttatgggct gccccgtctc tatatccagg ctggcaccca cgagctgcta
     1441 ctatcggata ttgcatcatt cgttgataag gcccggtggg caggagtccc tgtccagttc
     1501 gaattatggg agggaatgtt tcacagctgg caggtgtttg cccgggatat acccgaagga
     1561 cgggaggcga tcagccatat cggggcatat gccctggatg tcctgaaccg gtaatcgata
     1621 tccgtacttc tgaagaacga cccttgatgt tcttttctct tctgagagaa aaacgaataa
     1681 atcttcttca tattccacgt catatgtaca tcatatgttt gaaagcatcg gcagaagctt
     1741 tgcactcgtt aaaacgagct ggaatattct tatggatgat aaaaagctcc tcctgttccc
     1801 gatcatctca ggaatcgtga tgctctttgt cctccttaca tttattctcc cgctaattct
     1861 tgcaggcact tttatgaata tacccggtgt cggaccggtc tttttttatg gtctcctgtt
     1921 tgctttctac gtagccagtt acgttgttgg tattttcttc aatacagcat tgataacctg
     1981 cgtgaatgca cggttccagg gcagggatat ggcagtcggg gaaggactgt ccagtgcggc
     2041 acggcatcta ccatccattt tcttatgggc acttgtttct gcaaccgtgg gtgtcctcct
     2101 gcacctcatt caacggcggg caggtttcat aggaaggttt gctgcagcgc tggtgggtgg
     2161 tatctggagc cttgtcatgt tctttgttgt tcctgtcctc atacttgaag ataagggggt
     2221 agtggatgca gtgaaggaat ctgttgcact tatccggagg acctggggcg agagtattgt
     2281 gggttccggc tcaatcatgc tcattttcgt tttcatcggg ttattcggta tggtgtgcgt
     2341 ttttgcatcc ctgcttgtgg gcaatatgat tgtctttggg agtgcagttg tgctgttcat
     2401 catccttata gtccttcttg cgattgttgc ctcggcaatg cagggaatct ttattactgc
     2461 cctgtatacg tatgcacgga ccgggatcgt tccttcagaa ttcaatcagg atcagatccg
     2521 caatgcattc ataccgacac aggccggccc gggaactatc tgacctctct tttttccctt
     2581 ctgtatccgg gttgcacttg tgctgacgaa aagtatcatg caaaaaagca ccgggctgtt
     2641 gttgggatcg tatcagaacc ctgcaaatac gcatcaagga ataaacgaca ggagcttttg
     2701 atgggagtat gtatccatac ctttacactg cctgacttcc tacatttttt atggatactg
     2761 tcacagcatg ggaagcgttg cccggaaaaa cacaggacga gaaactcggc tcgcttggtg
     2821 tcacgaagga ccggatgctc gacattcgca tgctggatga gatcagccgt acatatggta
     2881 ttgagatttt tttattcttc gaagaagatc ttgcacgtac tcggaagctg gaatccgttc
     2941 ttaaggaatt tgagatcttt ccggagtttg gtcgtcccta catcaggatt gggagttttt
     3001 tacggtttac tcgggaaaac gatccgtctt ttgagcaaac cctgcgtgag tttcctctca
     3061 tggttgaaat cgtgaccatc ggagaaacac cgcagcccgg gcagggaaaa ccgatgcaat
     3121 acataacagg acttatgccg tttctggatg agctggatgt tgatgcagat cccgtcatct
     3181 gacctggata tctttttttt cagtatgctt tcgaaaaata cggggtcctc atggcaaaaa
     3241 gatcaaaaac gtacatctgg cagtttgtca taggtctggg ttttgtttcc gggctctgga
     3301 cagcgatagg tattaa
//
LOCUS       DASRLV010000031         8951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_9949, whole
            genome shotgun sequence.
ACCESSION   DASRLV010000031 DASRLV010000000
VERSION     DASRLV010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 8951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8951
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9949"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..974
                     /locus_tag="VFC43_01205"
     CDS             204..974
                     /locus_tag="VFC43_01205"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HZK29840.1"
                     /translation="MQYDKRCLMNHDQKTEMRYARNIEPVINSMQGLNRENILVRVFQ
                     HTSSEPLGYLESIFSGEGIPFEYVRLWEDDPVNMGSATHFVFLGGPMSVNDEVQLPWL
                     NEEKELIRRAAKKGSPVLGLCLGAQLIASAHGAPVYRYINETGWYPVHPVKDATGVFA
                     AFPDPFYVFQMHGETFHLPVRGRLQCRGDTVMHQGFRLGSALGLQFHLEMTENLIRDW
                     TKSERKFKKEKIGRDTKYFLETSNLLCRKVAVEFLYKK"
     gene            complement(988..1611)
                     /locus_tag="VFC43_01210"
     CDS             complement(988..1611)
                     /locus_tag="VFC43_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HZK29841.1"
                     /translation="MADIKGHIQDIPDSGKQKKRSTGIVGLNLLLDGGFPEGTIIMIY
                     GTPLAGVDLAASQFWKVEGEEGTYLMNDGDVELGMIDATDLHPEMYMGHMAGGRIVVD
                     SLSTIIIKYGIEEALKFLKLTREEMKKRNANIMFVVYTGIHAPIEMTRIMRASDIVIE
                     FKTDIHQAEIERSLAVHKMKDSASPRRLLPFIITDNGIEASTTSRVV"
     gene            complement(1705..3243)
                     /locus_tag="VFC43_01215"
     CDS             complement(1705..3243)
                     /locus_tag="VFC43_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HZK29842.1"
                     /translation="MSNVTTLLDAKSVPAVKTALIFPSRNEVYSYGKLREEMNRIGRG
                     LSTLGIKKGDRVCIYLDSSPEYLVSYFAIWRIGAVAVPTNIAYRGDELLHAIKDAGAS
                     AIITDCQGVNVVAGVRQNAPALAHIICVRCSCEGSTAWESFPIAPAAMRAVNCREDDL
                     CHIQYTAGTTGKPKGAMLTHGNWMTALDAEREALGLRQDDMYLGIYPMGHVGLSWGLS
                     MLRAGGTYIIMERFELDRYIDIAEQFGVTVLAGIPPVLHSLVHAPPGTEKRLTKARVI
                     ISGGGQLLPVVWQEFDHRYHIPVANSYGLSETIVIGSGTTTLPDYPHLTRNYLSVGVP
                     VGYTEVSIVDADDPEKELAPGERGEIALRGPSIAQGYWNLKEATAAVFRREGWFLTGD
                     IGYLDDEGILFITDRKKDMIIMSGWKIYPTEVENVIIQHPAIADVAVFGIPDERKGEV
                     PVAAIVLKLHTSITEAELGSFCCAHLAGYKIPRTMVIVDSLPRVHGWKLLRRTLREKF
                     GPQS"
     gene            3409..3711
                     /locus_tag="VFC43_01220"
     CDS             3409..3711
                     /locus_tag="VFC43_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29843.1"
                     /translation="MVNNEPQTQDIPGDPNQISAPSKNSGSQENSHFRRRGPDRRPEG
                     YESAPGKDTDLKEREGGSEEEEETEPERSSSQQRHHRGGRPEKRIIEESAYDPYCE"
     gene            complement(3820..6237)
                     /locus_tag="VFC43_01225"
     CDS             complement(3820..6237)
                     /locus_tag="VFC43_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HZK29844.1"
                     /translation="MPEVQLRVDSAYPGDQGGGKARLDPETMLLLKISPGDLVAIEGK
                     RRTVAKVWRALVEDWNQRKIRIDNFTRLNAGVSIGDTVRIVKIADEVEAKRVVLAPPE
                     DLPKKIPIANNPHVVNGLIDFPIAKNDSVPIMLGLPFIQPQIVAFKVVEIEPEEAVII
                     TKNTLVEFSDKPAAGFEGIKRFSYEDIGGLKDELQRLRETIELPLRHPELFQKLGIEP
                     PKGVLLYGPPGTGKTLIAKAVASESGAHFISIAGPEVISKYYGESEQRLREVFEEARE
                     NAPSIIFIDELDSIAPRREEVTGEVERRVVAQLLTMMDGLEERGQVVVIGATNRVDAI
                     DAALRRPGRFDREIEIGVPSEVDRIEILKIHSRGMPLAEDVKIETLAQQTHGFVGADL
                     AALAREAAIRALRRYLPDLDLDAEEVPSEVLDSLRVLASDFRSAQRDVGPSAMREVML
                     EVSHVKWENVGGLEEAKTEIREAVEYPLTHRQKFEDLGIEPPRGVLLYGPPGTGKTLI
                     AKAVASESGANFIPVRGPQLLSKWVGESERAVREVFKKARQVSPSIIFFDEIDALAPA
                     RGTSGDSHVIDNVLNQILTEMDGLEELKDVVVMGATNRPDIVDPALLRAGRFDRLVYI
                     GEPTFEDRKKIIHIHTRFMPLEGSALEEIMQLLEGYSEEAIGELVEKLGKDRVLSIDE
                     VKAQIIPDVNAKSGITLGIRRKRFIELLYEKNLVFTDPARDRIASELSGMTEGFVGSD
                     LESICREAGMLALREDATLVTQRHFELAQKKVHPMMNERLREYYQKIQQHFKGGLPKQ
                     VQPPEYQ"
     gene            6280..7500
                     /gene="larC"
                     /locus_tag="VFC43_01230"
     CDS             6280..7500
                     /gene="larC"
                     /locus_tag="VFC43_01230"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HZK29845.1"
                     /translation="MLVLVFDPFHGAAGDMIIGALLDCGADKKSVLKAMQSVIAEPGI
                     TEVSRAGIRAVKVATHATPNPRTLSEVMQRLCEAEDDVPKEALAMAKRIFKRINDAEE
                     RVHGSHTHFHEVGADDAIADVIGACTALYSLRLDGVAVMPIALGSGSVNGSHGTFPVP
                     APATTEILRQSKLLIREGKEEGELCTPTGAALLAEFSTISPSELGTYSVKGVGYGAGT
                     KNPAKTPNVIRAMIVETIGTTGPMTMDTVDILETNVDDVSGEVIANAITRFLDAGARD
                     ASAIPIIMKKGRPGFLIRVICSSEKSSELAEHMARELGTLGVRCFPAIHRFIADRTIE
                     PVTVEISGNTRVIPVKYGRIRGEVYTLKAEFDQARIWAEELGIPVHMVLRAVEKAGWK
                     SAGKKDLIGGTNED"
     gene            7490..8167
                     /gene="radB"
                     /locus_tag="VFC43_01235"
     CDS             7490..8167
                     /gene="radB"
                     /locus_tag="VFC43_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012108006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadB"
                     /protein_id="HZK29846.1"
                     /translation="MKTDRRSAGSPALDHLIGGGLEVRTITQFYGEPASGKSTLCMIA
                     AVACLRAGQTVIYIDSEGFSIERFRQIAGTDTEEIADRLFLFEPFDFEHQGTMIAEAE
                     KILKTKKPGLLVMDSATALYRTDLEKGRDSIQMLTRQMIQLLGYSKRYEIPVIITNQV
                     YMDTMKNTYFGLGGTALEHLSKVIIRLEKTDTPGFRRARLVKHRSQPEGAYFDFEITG
                     DGIRTRE"
     gene            8268..>8951
                     /locus_tag="VFC43_01240"
     CDS             8268..>8951
                     /locus_tag="VFC43_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZK29847.1"
                     /translation="MVLKKEITLQIKEILQKNPQGLSITDIVKEVNINRNTAGRYLEN
                     ILVSGQVDMRRLGMAKIYMMSQRVPVSAVLSISSELVMQLDSSLRIVFANEPFLKLAG
                     TDSKNLLGKNIEYSPVQLVFDEFFTRFLENIRAGITGKEWSGEIVLPLKDIILFCQIA
                     PTVFDDGRKGVSVILQNITEKKKAELRVEESERQFRLLAENSLDMIGRIKPDFTHMYA
                     SPAYTTTLGY"
BASE COUNT         2335 a         2199 c         2173 g         2244 t
ORIGIN      
        1 tgtggcattt gctaaactga agcatatgac tgaagcggcc aggatggtcc gcattggtct
       61 ttgatttttc tgattcgggc ttttttatga aatcctgtat gtaagaccga tcatgcatga
      121 ggattgctgt cgaacagcaa attctcctta ataaaaacat cgatcgcaat atccgttttt
      181 tgtaccaata ttgaggtgtg ttattgcagt atgacaaacg ttgcctgatg aaccacgacc
      241 aaaaaaccga aatgcgttac gccagaaata ttgaaccggt aataaattct atgcagggac
      301 taaacagaga aaatattctg gtcagggttt tccagcatac atcctcagag ccgctcggtt
      361 accttgagag tatcttttca ggagagggga taccgtttga atatgtacgg ctgtgggaag
      421 atgaccctgt taatatggga tcagctaccc actttgtttt cctcggcggg ccgatgagcg
      481 tgaacgatga ggtgcagcta ccttggctca atgaggagaa agaactaatc cggagggcag
      541 caaaaaaggg aagtcccgta ctcggcctgt gccttggtgc ccagctgata gcatcggcac
      601 acggagcccc ggtgtatcgg tatatcaacg aaacagggtg gtatccggtg caccctgtaa
      661 aagatgcgac cggagtcttt gcagccttcc cggatccttt ttacgtattc cagatgcatg
      721 gtgagacctt tcatctccct gtcagaggca gacttcagtg caggggggat actgttatgc
      781 accagggttt caggcttggg tcagctctcg gtctccagtt tcaccttgag atgactgaaa
      841 acctgatcag ggactggact aaaagcgaga gaaaattcaa aaaagaaaag atcggacgcg
      901 atacaaaata tttccttgaa acgagcaatc ttctctgcag gaaagtagca gtggaatttt
      961 tgtataaaaa ataaaagagt tctgaattta aactacacgg gaagtagtcg atgcttcgat
     1021 tccattatcg gtaataatga agggtaacaa tctccgtggg gatgccgaat ccttcatctt
     1081 atgcacggca agagatcgct caatctcggc ctgatgaata tcggtcttga actcaatgac
     1141 aatatctgat gcacgcatta tcctggtcat ttctataggt gcgtgaatcc ctgtgtaaac
     1201 gacaaacatg atgtttgcgt tacgtttctt catttcttca cgggtaagct ttaaaaattt
     1261 gagtgcttct tcgataccgt acttgatgat aatggtcgag agcgaatcaa caacaatcct
     1321 gcctcccgcc atgtgcccca tatacatttc agggtgaaga tccgtggcgt cgatcatccc
     1381 gagctcgaca tccccatcgt tcatcaggta ggttccctct tcaccttcaa ccttccagaa
     1441 ttgtgatgcc gcgagatcaa caccggcgag cggtgtccca taaatcatga taatggtccc
     1501 ttcaggaaac ccaccatcca gtaaaaggtt gagaccaaca attccggtac tccgtttttt
     1561 ttgttttcca ctatccggaa tgtcctggat atgacccttt atatctgcca aaatgcttct
     1621 gccatatgtt tttttttccg ctttaaaagg tttgtgtgaa aaatgggcag tctgttgagg
     1681 atttgaaaag gtctttttcc cgcatcagga ttgaggacca aatttttccc gcaatgttct
     1741 gcgcagcaat ttccacccgt gaacccttgg taatgaatcc acaatcacca tagtgcgggg
     1801 tatcttatat ccggcaaggt gtgcacaaca aaaagagcca agttctgctt ccgtaatcga
     1861 agtatgaagt ttgagtacaa ttgctgcaac cggtacctcc cctttacgtt catccggaat
     1921 tccaaaaaca gcgacatcgg cgattgccgg gtgctggatt atcacgttct cgacttctgt
     1981 ggggtagatc ttccacccgg acatgatgat catatctttt tttctgtcgg tgatgaagag
     2041 gataccctca tcatcgagat acccgatatc tccggtaagg aaccacccct ctctgcggaa
     2101 tactgctgca gtcgcctcct taagattcca gtacccttgt gcaattgagg gaccgcgaag
     2161 tgcaatctca cctctctctc ctggtgcaag ttctttttcc ggatcatcag catcaacaat
     2221 cgatacttca gtataaccaa ccgggacacc cacactcagg tagttcctgg taaggtgagg
     2281 gtaatccggg agggtggtag tacccgatcc gattacaatc gtttccgata aaccgtaaga
     2341 attggcgacc ggaatgtgat accggtggtc aaactcctgc cagacaacgg gcaggagctg
     2401 ccccccgcct gaaataataa cccgtgcttt ggtgagacgt ttctcggttc ctgggggtgc
     2461 atgaacaagc gagtgaagaa ccgggggaat acctgcgaga acagtaaccc cgaactgttc
     2521 tgcaatatcg atataacgat cgagctcgaa gcgctccata atgatatacg ttccacccgc
     2581 acggagcatg gacagtcccc acgagagccc tacatgcccc atcgggtaaa ttccaagata
     2641 catatcatcc tgccggagcc ccagtgcttc acgttctgca tcaagtgccg tcatccagtt
     2701 gccatgagtg agcatcgctc ccttgggttt tccagtagtc ccggctgtgt actggatatg
     2761 acagagatca tcttccctgc agttcactgc ccgcatggcc gctggcgcga tcgggaatga
     2821 ttcccaggct gtcgaacctt cacaagagca tcggacacag ataatatgag caagtgccgg
     2881 ggcgttctga cggacaccgg caacaacatt taccccctgg cagtctgtaa tgatcgcaga
     2941 agctcctgca tctttgattg catggagcag ttcatccccg cgatatgcta tattggtagg
     3001 aacagcgaca gcgccgattc gccagatcgc aaaatagctg accaggtact caggagaact
     3061 gtcaaggtag atacagaccc tgtcaccctt tttgatacca agtgttgaga gcccccggcc
     3121 aatccggttc atttcctccc gcagctttcc gtaactgtac acctcatttc tggaggggaa
     3181 gataagggca gtttttacag cgggaacact ttttgcatca aggagagtgg taacatttga
     3241 catgcgacat caggtgaata cgatgaccca aaatttgatg ttgatgtatt taggttttca
     3301 taatcagatg tctggaatca tcaaagacaa aaaacgtcaa tgccgattcg ttttttttgt
     3361 atatgctaat gtatttctgg ttactaatcc gattatcaat cgataattat ggtaaataat
     3421 gagccccaga cacaggatat ccctggtgat ccgaaccaga tatcagcacc ttcaaaaaat
     3481 tccggcagcc aggaaaattc tcattttcgg cgaagagggc cagaccgccg tcccgaaggt
     3541 tatgagtccg caccgggcaa ggatacagat ttaaaagaga gagagggtgg ttctgaggag
     3601 gaggaggaaa ccgaacctga acggtcatct tcccagcaac ggcaccacag agggggcaga
     3661 ccggaaaaga ggattattga ggagtctgcc tatgatcctt attgtgaata aagtgccctg
     3721 ctgacatctt tgggataacc cgtgtcagta taagacgcaa tcgtacgcga aaaataagga
     3781 ttgcgggtta agtcccagtc gtcataaaaa ataaaaagat tattggtatt ccggcggctg
     3841 cacctgtttg gggagaccgc ctttgaaatg ctgctggatc ttctggtaat actcccgcag
     3901 cctttcattc atcatcggat gcactttctt ctgtgctaac tcaaaatgtc gctgtgtcac
     3961 caaagtcgca tcttcacgca atgcgagcat accggcttcc ctgcatatcg attccagatc
     4021 cgaaccgaca aatccctcgg tcatccccga aagctctgat gcaatccggt ctcgtgcagg
     4081 gtctgtgaag acaaggttct tctcatataa tagttcgata aaacgctttc tgcggattcc
     4141 gagggttatg ccggatttgg catttacgtc tggaatgatc tgtgccttaa cctcatcgat
     4201 tgacaaaacc cggtcttttc ccagtttctc aacaagctct ccgattgctt cctcgctgta
     4261 tccttccagc agttgcatga tctcttccag tgctgaacct tcaagaggca tgaaccgcgt
     4321 gtgaatatgg ataatctttt tcctgtcctc aaatgtgggt tccccgatat acacaagacg
     4381 gtcaaaccgg cccgcccgca agagtgccgg atcaacaata tccgggcggt ttgtcgcgcc
     4441 cataacgact acatctttga gttcctcaag accatccatc tcggtcagga tctggttaag
     4501 aacattgtca ataacatgcg aatccccgga tgtgccccgt gcaggagcca gagcgtcgat
     4561 ctcatcgaag aaaataattg acggggatac ctgccgtgcc tttttgaata cttcgcggac
     4621 agcccgctca ctttccccta cccatttgga aagaagctgg gggcccctga ccgggatgaa
     4681 attcgctccg ctttcgcttg cgactgcctt ggcgatcagc gtcttacctg tcccaggcgg
     4741 accataaaga aggactcccc tggggggttc aataccaaga tcttcgaatt tttgccggtg
     4801 ggtaagggga tactcaactg cttcgcgtat ttcggttttt gcctcttcaa gcccgccaac
     4861 attttcccat ttcacgtggg atacttcgag catcacttca cgcatcgcgc tgggcccgac
     4921 atcccgctgg gcgctgcgga agtcactggc aagaacgcgc aatgaatcca ggacttctga
     4981 cgggacctcc tcggcatcga gatccaaatc ggggagatag cggcgcaaag cccggattgc
     5041 tgcctcccgg gcaagtgctg cgagatccgc accaacaaaa ccgtgcgtct gctgcgcaag
     5101 agtttcaatc ttcacatctt cagcaagcgg catgccgcgg gaatggatct taagaatttc
     5161 aatccggtcc acctcgcttg ggacaccaat ctcaatctcc cggtcaaacc ttcccggtct
     5221 ccggagggcg gcatcgattg catcaacccg gtttgtcgca ccgataacaa caacctgtcc
     5281 tcgctcctcc agcccatcca tcatggtgag gagctgggcg acaacacggc gttctacttc
     5341 tcccgttact tcttcccgcc gtggcgcaat ggaatcgagt tcgtcaatga aaataatcga
     5401 cggagcgttc tcccgtgctt cctcaaagac ttcacggagc ctttgttcgc tctcaccata
     5461 gtatttcgat atgacttctg gaccagcaat tgatatgaag tgtgcaccac tctcactggc
     5521 aactgctttt gcaataagcg tcttgccggt tcccggaggg ccataaagaa gcactccttt
     5581 tggtggttca atcccaagtt tctggaataa ctcgggatgc ctgagcggca gctcgatcgt
     5641 ctcacgcagc cgttgcagtt cgtctttgag cccgccaata tcttcgtagg agaaacgttt
     5701 gatgccttca aaacctgctg cgggtttgtc ggagaactca actaaggtat tcttggtgat
     5761 gattaccgct tcctcgggct caatttccac gactttaaaa gccacgatct gcggttgtat
     5821 gaagggtaag ccgagcatga ttggcacgga atcgtttttc gcgatgggaa aatcgataag
     5881 cccgttcaca acatgcgggt tgttggcgat cggtattttt ttcggaagat cttcaggagg
     5941 tgccagcaca acccgctttg cttccacctc gtcagcaatt ttcacgatcc tgacggtgtc
     6001 accgatgctg acaccggcgt tgaggcgggt aaaattatca atccgtatct tgcgctggtt
     6061 ccagtcctcg acaagcgcac gccagacctt ggcaaccgtc cggcgtttcc cttcaatcgc
     6121 cacaagatca cccggtgaga tcttgaggag taacatcgtc tcgggatcaa gcctggcttt
     6181 tccgccaccc tgatcaccgg gatacgcaga atccaccctc aattgaactt caggcattac
     6241 cttaaaggtc atatacgcag ggatttataa gtactgtaaa tgctggtcct tgtttttgat
     6301 cccttccacg gtgcagcggg cgatatgatc atcggagcac tgctcgactg tggggcggat
     6361 aaaaaatccg tactgaaggc aatgcaatcc gtgatcgcag aacccggtat caccgaagta
     6421 tcccgggccg gtatcagggc agtgaaagta gctacacacg ccaccccaaa cccccgcaca
     6481 ttatctgaag tcatgcaacg actttgtgag gctgaggatg acgtcccaaa agaagcactc
     6541 gcaatggcaa aacgtatctt taaaagaatc aatgatgctg aagagcgggt tcatggcagc
     6601 catactcact ttcatgaggt tggagcagac gatgcgatag ctgatgtgat tggagcctgc
     6661 actgctctat attcacttcg tcttgatggt gtagcagtaa tgccaatcgc cctgggaagt
     6721 gggtccgtga atggatctca cggcactttt cctgtaccag caccggccac taccgagatt
     6781 ctcagacaat cgaaattgtt gatccgggaa ggaaaagaag agggggaatt atgtactcca
     6841 accggagccg cactccttgc agagttctcc acgatatctc cgtcagaact tggaacatat
     6901 tcggtgaagg gtgttgggta tggtgccgga acaaaaaacc cagctaaaac acccaatgtt
     6961 atcagggcaa tgattgttga aacaatcgga actacgggtc cgatgacaat ggatactgtt
     7021 gatattctcg agacaaatgt tgatgatgtc agtggtgaag tgattgccaa tgccattacc
     7081 cgttttttag atgctggtgc acgggatgcg agtgcaatcc cgattattat gaaaaaagga
     7141 agacccggtt tccttatccg ggttatctgc tccagtgaga agagttcgga acttgctgag
     7201 cacatggcac gggaactggg aacgctgggt gtccggtgct ttccagcaat acaccgtttt
     7261 atcgccgacc ggacaattga accggttact gttgaaatat ccgggaacac acgggttata
     7321 ccggtcaaat acggaaggat aaggggcgag gtctatacac tcaaagcaga atttgaccag
     7381 gcaaggatct gggcagaaga gctgggaatt cccgtgcata tggttttacg tgcagtagag
     7441 aaagccggct ggaaatccgc aggaaaaaag gatctgatcg gggggactaa tgaagactga
     7501 tcgacggagt gcgggtagtc ccgcccttga tcatctgatc ggcggcggac ttgaagtacg
     7561 gactatcacg caattttatg gggaacctgc cagtggaaag agtaccttat gcatgattgc
     7621 ggcagttgcc tgtcttcgtg cgggtcagac agtgatatat atcgattcgg aaggattttc
     7681 cattgagcgt ttccggcaga tagcaggcac tgacaccgaa gaaattgccg acagattatt
     7741 tttgtttgaa ccatttgact tcgaacacca gggcaccatg atcgccgaag cggagaaaat
     7801 tctcaagaca aaaaaacccg gattgctggt tatggattct gcgacggcat tataccgaac
     7861 agatcttgag aagggcaggg attcaatcca gatgcttacc cgccagatga tccagctgct
     7921 gggttattcc aagcggtatg aaatcccggt aattatcacc aatcaggtat acatggatac
     7981 catgaaaaac acgtattttg gtcttggagg gactgcactg gaacatttat caaaagtaat
     8041 tatccggctc gaaaagaccg atactcccgg gttccgaagg gcacgactcg ttaaacatcg
     8101 ctcgcagccc gaaggggcat actttgattt tgaaataacc ggcgatggga tcagaacacg
     8161 agagtagtcg ggaattttga tcataatacc tgataatctc tcaattgttg cttcaactgg
     8221 caatatctgc attaatatat gctgacaagc caatagaatt ctcaaacatg gttctgaaaa
     8281 aagagattac actgcagata aaagagattc tgcaaaaaaa tccgcagggc ctgagtatta
     8341 ctgatattgt caaagaagtc aatatcaaca ggaacactgc cggacgatac ctcgagaaca
     8401 ttctcgtttc gggacaagtg gatatgcgcc gtctcggaat ggcaaaaatc tacatgatgt
     8461 cgcagagagt cccggtctcc gcggtactat cgatatcttc agaactggtc atgcagctgg
     8521 acagcagcct tcggatcgtc tttgcaaacg aaccttttct caaactggca ggaactgaca
     8581 gtaaaaacct gctcggaaaa aatattgaat attcgcccgt tcaactggtc tttgatgaat
     8641 tctttaccag gtttttagaa aatatcaggg cgggaataac cggtaaggaa tggtctggcg
     8701 agattgtact tcctttaaaa gatatcatcc tgttttgtca gatcgccccg actgtttttg
     8761 atgacgggcg taagggagtg tcagtcatcc ttcagaatat cacggaaaag aagaaggccg
     8821 aactgagagt ggaggagagc gaacgacaat tccggttgct ggcggaaaac tccctcgata
     8881 tgattggccg gatcaaaccg gattttaccc atatgtacgc ttcacccgcc tacacaacca
     8941 cgcttggcta c
//
LOCUS       DASRLV010000032         9146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_10093,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000032 DASRLV010000000
VERSION     DASRLV010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 9146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9146
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10093"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VFC43_01245"
     CDS             complement(<1..584)
                     /locus_tag="VFC43_01245"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZK29848.1"
                     /translation="MHLQSLRLLHDTFPTRNCYPFNLEIFQKTEGIEFTRPVTFFIGE
                     NGTGKSTLLRAIARSCSIHIWEEHEGTKDRQNRYEDLLHKSIRITWTDGKVPGSFFAS
                     EIFRHFTEILDQWGMADPDTLKYFGDGTLIVKSHGQSHMKFFETRFKRKGLYLLDEPE
                     NALSPRMQLELLRLLRQFSTRGDVQFIIATHSPIL"
     gene            complement(763..1041)
                     /locus_tag="VFC43_01250"
     CDS             complement(763..1041)
                     /locus_tag="VFC43_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29849.1"
                     /translation="MHDTYSPPLWAVMGCLANILVSVAMIWYLIIFLSSCTTCAARPF
                     AYAFFVGFLMILLLTGFELFKLLFAGRKKTRRDGSMVARIIEIDGKVN"
     gene            complement(1317..3743)
                     /locus_tag="VFC43_01255"
     CDS             complement(1317..3743)
                     /locus_tag="VFC43_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZK29850.1"
                     /translation="MSAIGGKTGIRQVMFSVLYVDDEPLLLELAKLFLEKTGDFRIDT
                     LTSAKAALETLARTSYDCIISDYQMPGMDGIVFLKTVRSHGSTIPFIIFTGKGREDVV
                     IEALNSGADFYLQKGGDPKAQFVELAHKIRQAIQIKGAQKTMQDLVQGAPIPEFVIDK
                     SHRVIFWNRALEEYSGLAASEIVNTTNHWKAFYPTQRPCLADLIVDGNIDGITRWYAG
                     KHVKSPVVADNFEATDYFPSIKGGTWLFFTAAPIRDPSGNITGAIETLQDVTSLRKKE
                     EELRAACEQMEAAFEHAKASEAALNIQHKRLEASEQRYRDVVEDQTEFISRFLPDGTH
                     VFVNEAYCRYYHKTREEIIGKKFIPEVPAEDRVLLKEHFASLTRDHPLAIITHRIIAD
                     GGEVRWQRWSDRAIFDENGTLTEYQSVGSDVTEQKRADEALRESEASLASIFRVAPVG
                     IGLVSDRVIVRVNDRISEMTGYSYDDLVGQSARILYPTDEDSDFVGKVKYDMIRKQGA
                     GTVETRWLRKDGVIRDILLSSTPVNPPDISAGVMFTALDITDRKNAEAELHTAYEQLA
                     KTEEELRKQYSELAHAKESLKEANRKLHLLSSITRHDVLNKITVLRGNIELIRSRSGN
                     PAIAGFLTKLDSAAIAIREQIEFTRIYQDLGSHEPRWHKLDMVISSLEVPPNISLRQS
                     LDHIEIYADPILEKVFYNFLDNSVMHGKKVTTITVSSREFPEGLVISWEDDGIGIPAE
                     EKKKIFTRFFGKHTGLGLFLAHEICSVTGITLRERGEPGKGALFEMLVPKTAYRFTGT
                     SEKNLLVGRD"
     gene            complement(3819..4580)
                     /locus_tag="VFC43_01260"
     CDS             complement(3819..4580)
                     /locus_tag="VFC43_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK29851.1"
                     /translation="MTDGSGAYGQIYAELNAAWAIAKKDIAIYYFKPNIIVSGALFPL
                     FMFLAFAAGKSAGPSVMIPGLIAITILFSASSIEPVSIPIERRMKTFDRLISAPVSLH
                     AVVLGESLSGFLYSMCIAIVPLIAGLLIFGTPVVSAAPLIAGIILTSFCFATMGTLFA
                     AYPTESPGDIMSMLNLVRLPLIFISGVFIPIEAIPSIGQGAAYFSPLTYGNDLIQAAY
                     HGHTYFDPLLDIAMLCIFILIFQIFANQLYRKWNE"
     gene            complement(4573..5529)
                     /locus_tag="VFC43_01265"
     CDS             complement(4573..5529)
                     /locus_tag="VFC43_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZK29852.1"
                     /translation="MNAIEVDHLSKHFGALVALNDISFSVAEGETFGFLGPNGAGKTT
                     TIRLLTGISPPTAGTAKIFGKDIVRETTAARKQMGIVHETSNIYDDLSAWQNLMFTAE
                     LYTIGKKEREQRGKELLDLFSLWDRRTDKVHGFSKGMKRRLTLAMGLINNPRLLFLDE
                     PTSGLDVQSNLIIREVIRDLTSRGVTVFLTTHNIEEANLTCDRVAIINRGQIAAIDSP
                     ERLKATIQSAQSVEVAFDRTAAGRENDLRHLHGVSEVRKEGDKFRLFTHNPPDVITAV
                     MEYAQVHESRVLSMTTFGPSLEDVFIRLTGLEVRTKGVSTVD"
     gene            complement(5714..6583)
                     /locus_tag="VFC43_01270"
     CDS             complement(5714..6583)
                     /locus_tag="VFC43_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZK29853.1"
                     /translation="MFEKIIFPTDFSKPARTELNCITSIPGIREIILLHVIQQYAIPM
                     GVETIENLELETTEVYLHEAKAYIASLNPAIRVTLEETIATDITGAILEKAEEHHAGL
                     IVIHAHIKGIIAGVLLNCVSSKVLCRISKINVMIMPNTLVGSLTGKTYEKFCPMIFSR
                     VLCPTNFSEFSEKTVALVSTIKGMGEIILLHVVQKGEEKGDHTLALTTAEQRIHAVCD
                     RLSAQGINARTIVVTGKPVIEITRVAQAEDVSLIWMRSAAQGCLHDFFFGSMVHDVVM
                     NATRPVIVIRSYV"
     gene            complement(6760..7176)
                     /locus_tag="VFC43_01275"
     CDS             complement(6760..7176)
                     /locus_tag="VFC43_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29854.1"
                     /translation="MAPEDTDPAAHPGCNHDQKGREKVHTRIKKHQVPAQKITGADIY
                     GPEDIPPEVKCNYPGWEAYLEQYPMVHSLQREDFPDHGVSFVYLAHFYRTKNTADESS
                     LAQKEFEAFFSKIGYEYDYSGNFESRWFEGIGVKIN"
     gene            7418..7624
                     /locus_tag="VFC43_01280"
     CDS             7418..7624
                     /locus_tag="VFC43_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29855.1"
                     /translation="MVRGERESDRRGGEGYMGRGMRGSNDDRWDPGDLERDPYRDLKD
                     GFEDLTDPLNDEYIWPSTEEEEDD"
     gene            7840..8691
                     /locus_tag="VFC43_01285"
     CDS             7840..8691
                     /locus_tag="VFC43_01285"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HZK29856.1"
                     /translation="MRWRDGRRSDNVEDQRGFGVKQGAIGGGIGILALLVIGLLTGAD
                     PVSLISAIVTQSGSLDSTPVVLTAAENESADFVSVVLADTEDTWQSIFQQNGQVYRQP
                     KLVLFKGSVESACGNAQAAMGPFYCPGDEKVYIDLDFYNELKNQLGAPGDFAQAYVIA
                     HEVGHHVQKLDGTSAEVQRLESQSDKVTANKYSVMLELQADCYAGIWAYHADKQRQMI
                     EAGDIGEAMNAAAKIGDDALQKQSQGYVVPDSFTHGTSAQRMKWFNAGYVSGGVGSCD
                     TFNAAAL"
     gene            complement(8789..>9146)
                     /locus_tag="VFC43_01290"
     CDS             complement(8789..>9146)
                     /locus_tag="VFC43_01290"
                     /inference="COORDINATES: protein motif:HMM:NF024924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZK29857.1"
                     /translation="SAQSGLANSLTKTELSLFKKVKKELEKRVLIPCTGCRYCTPCPH
                     GVSIPECFEYYNRGHMYEDAEQTKQIYTMFLGGFFDGIPHYASLCQECGECEEKCPQD
                     LPIRDNLKKVVDYFGK"
BASE COUNT         2239 a         2195 c         2351 g         2361 t
ORIGIN      
        1 aggatagggg aatgagtggc gataatgaac tgcacatctc cccgggtgct gaactgtctt
       61 aacaagcgca acagttcgag ctgcatcctt ggtgacaggg cgttttccgg ttcgtccagc
      121 aggtacagtc cttttctctt aaagcgggtt tcaaaaaatt tcatatggga ctggccatgc
      181 gactttacta tgagagtccc gtcgccgaaa tacttcaggg tgtcagggtc agccatgccc
      241 cactggtcga ggatttcggt gaaatgacgg aaaatttctg aagcaaagaa cgatcccggg
      301 accttgccat cagtccatgt gatccttatg gacttatgca ggagatcttc atagcggttc
      361 tggcggtctt ttgttccctc atgctcttcc cagatatgga ttgaacagct gcgggcgatg
      421 gcgcggagga gcgttgattt tccggtcccg ttctccccga tgaaaaaggt cacaggccgg
      481 gtaaattcta tcccttcagt cttctgaaaa atttccaggt taaacgggta acaatttctt
      541 gtcggaaagg tatcgtggag cagacggagc gattgaagat gcatatcctg attgaatttt
      601 ttatgacaca gaatttatat ctttcattcc ggtttttatc cggttaccgc gttatgtccc
      661 cttgcatcat agagagttgc cagaatctga tccaggatga tcccttcgcg aaaacagggg
      721 gaaatgagga aaagcggtag agcagagtat cccggtatga ccttagttta ccttgccatc
      781 gatctcgata attcttgcca ccatggaacc atccctgcgg gttttcttcc ggccggcaaa
      841 taatagtttg aacagctcaa aaccggtgag cagcaggatc atcaaaaatc cgacaaaaaa
      901 cgcataggca aacggtcttg ctgcgcaggt tgtgcacgag gataaaaaga ttataaggta
      961 ccatatcatc gcgacagaga cgaggatatt ggcaagacat cccatcaccg cccacaacgg
     1021 tggggaatag gtatcatgca tacaatgtcg ttgttttact tttccggtac atatagtatc
     1081 tcattgtgga atagtggcac tattcttctg gtttttcctc catctcctcc ttgtttttct
     1141 taaagttccg ggcaagggca atcacgcccg ttgtgtcccc gtcaaccagt ggggagactg
     1201 attggcgcag gagagggtaa aaaaaaataa tcagtctgat tagtttgaga attaaaaata
     1261 tcagaatgta ttccggtgat caggtttatc agtccatctc gatcaggttc ccgatctcaa
     1321 tccctgccaa ccagcaggtt tttttcggag gtcccggtga accggtacgc agttttgggt
     1381 accagcatct caaacagtgc tcctttgccg ggctcccccc tctcgcgcag tgttataccg
     1441 gtcactgaac agatttcatg ggcaaggaac agtccaagcc cggtgtgctt cccaaaaaac
     1501 cgggtaaaaa ttttcttttt ttcctctgcg gggatcccga taccatcatc ttcccaggaa
     1561 attaccagcc cttcggggaa ttcacgggaa gacaccgtaa tcgttgttac tttcttcccg
     1621 tgcataacgg aattatcaag gaaattgtaa aacaccttct ccagtatcgg gtctgcgtaa
     1681 atctcgatat ggtccaggga ctggcgaagt gaaatgttcg gggggacctc caggctggaa
     1741 atcaccatgt ccagtttgtg ccaccgtggt tcatgggatc ccaggtcctg gtagatccgc
     1801 gtgaattcga tctgctcgcg tatcgcaatt gcggcagagt ccagttttgt taaaaacccc
     1861 gcaattgccg ggttgcctga cctggatctg atgagctcaa tgtttccccg caggaccgtg
     1921 atcttgttga gaacatcatg acgggtgatg ctggacagga ggtggagttt acggttggct
     1981 tccttgaggg attcttttgc gtgggcaagt tcgctgtact gtttgcgtaa ttcctcttcg
     2041 gtctttgcga gttgttcata ggccgtatgc agttcggctt cagcattctt tcggtcggtt
     2101 atgtcaaggg cagtgaacat gactcctgca ctgatatcgg gaggattaac cggggtggaa
     2161 ctgagcagga tatcccggat aacgccgtct ttccggagcc agcgggtctc gactgtcccg
     2221 gctccctgtt tccggatcat atcgtatttc acttttccca caaaatcaga atcttcatcg
     2281 gtcggataca ggattcgggc gctttgaccc accaggtcat catacgaata cccggtcatt
     2341 tcactgatcc ggtcattcac ccgcacgatg acacggtctg acacaagacc gatcccgacc
     2401 ggtgcgaccc tgaaaatgct ggcaagtgag gcttcactct cgcgcaatgc ctcatctgcc
     2461 cgtttttgtt ccgtgacatc gctgccaacg gactggtatt cggtgagggt tccgttctcg
     2521 tcaaaaattg cccggtcgct ccagcgttgc caccggacct cgccgccatc tgcgatgatg
     2581 cggtgggtga taatggcaag gggatggtcc cgtgtcagcg atgcaaaatg ttccttgaga
     2641 agcaccctgt cctctgccgg aacttccggg atgaattttt tcccgataat ctcctcgcgg
     2701 gttttatgat aataccggca atatgcctca ttgacaaaga catgggtccc gtccggcagg
     2761 aaccgggaga taaattctgt ctggtcttca acaacatcgc ggtatctctg ttcactcgcc
     2821 tcaagccgtt tatgctggat attgagcgct gcctcgctgg cttttgcgtg ttcgaatgcc
     2881 gcctccatct gttcgcaggc agcccgcaac tcctcttctt ttttccggag cgaggtaaca
     2941 tcctggaggg tttcaatggc cccggtaata tttccggagg ggtcacggat gggtgcagca
     3001 gtgaaaaaca gccaggtccc tcctttgatt gaggggaaat aatcagttgc ttcaaaatta
     3061 tccgcaacaa ccggggattt tacatgtttt cctgcatacc accgggtgat gccgtcgatg
     3121 ttcccgtcta cgatgagatc tgcaagacac gggcgctgag tgggataaaa tgctttccag
     3181 tgatttgtgg tatttacgat ctctgatgct gccaggccgc tgtattcttc aagagcccgg
     3241 ttccagaaaa ttacccggtg acttttatcg ataacaaact ccggtatggg cgctccctgc
     3301 acgaggtcct gcatggtctt ttgcgctccc ttgatctgga tggcctgccg tattttatgg
     3361 gcaagttcaa caaactgtgc tttcggatcg ccccctttct ggagatagaa gtcagcaccg
     3421 ctgttgagag cctcaataac aacatcttcc cggcctttcc cggtaaaaat gatgaaggga
     3481 atggtactac catgagaacg aacggtcttt aaaaaaacaa taccgtccat acccggcatc
     3541 tggtagtcgg atatgataca atcgtatgaa gtccgggcaa gggtttcaag agctgctttg
     3601 gcagacgtga gcgtatcaat cctgaaatct ccggtctttt caagaaataa tttcgccagt
     3661 tcgagcagca ggggctcatc atcaacatag agaactgaga acataacctg acgtattcct
     3721 gtttttccgc cgattgctga cattacattt ggaatgattg atatatataa ttccacgtgg
     3781 atctgtcctg caacgggttt gtacaggtta ctttgcgctc attcattcca tttcctgtac
     3841 aactggtttg caaagatctg aaagatcagg ataaagatgc agagcatggc gatatcgagg
     3901 agcgggtcga aataggtgtg cccatgatat gcagcctgga tcaggtcgtt accataggta
     3961 agcggagaaa aatacgccgc accctgccca atggaaggga tagcctcaat cgggatgaat
     4021 accccggaga tgaaaataag gggcagcctg acgaggttga gcatggacat gatatcaccg
     4081 ggactctcgg tagggtatgc ggcaaaaagg gtccccatgg ttgcaaaaca aaatgaagtg
     4141 agaatgatgc cggctataag gggggcggca ctgacaaccg gtgtcccgaa gatgagcagc
     4201 cctgcaatca ggggaacgat cgcgatacac atgctgtaca ggaacccgct gaggctctcc
     4261 ccgagcacca ccgcgtgcag ggatacaggg gctgaaatca gccgatcgaa tgtcttcatg
     4321 cgtcgttcaa tgggaatgga taccggttca atcgaagatg cggagaagag tatcgtgata
     4381 gcaataagcc ccgggatcat taccgatggc ccggctgatt tcccggcagc aaaggcaagg
     4441 aacatgaaca ggggaaacag tgcaccggag acgatgatgt ttggcttgaa gtaatagatt
     4501 gcgatatctt ttttcgcaat tgcccatgcc gcgttcagtt cggcatagat ctgcccatat
     4561 gctcctgacc cgtcagtcaa cggtgctcac tccttttgtc cgcacttcaa gcccggtcag
     4621 cctgataaaa acgtcctcaa gactggggcc gaacgtggtc atactgagca cacgggattc
     4681 gtgcacctgt gcatattcca taactgcagt aattacatcg ggcggattat gggtgaacag
     4741 acggaacttg tccccttctt tcctgacttc acttacccca tggagatgcc gcaaatcatt
     4801 ctcacgaccg gctgccgtcc tgtcaaaggc aacttcaact gactgggcgc tctggatagt
     4861 ggctttcagc cgttcagggg aatctattgc agcaatttgt ccacggttga taatggcgac
     4921 acggtcgcag gtcaggtttg cctcctcgat attgtgggtc gtgagaaaga cggtaactcc
     4981 ccttgatgtc aggtcacgga tgacttcccg gatgatgaga ttgctctgga catcgagacc
     5041 cgaagtgggc tcatcaagga acaggaggcg gggattgttg ataaggccca ttgcgagggt
     5101 aaggcggcgc ttcatgcctt ttgagaaccc atgaactttg tcggtcctcc gatcccagag
     5161 tgaaaaaagg tcgaggagct cttttccccg ctgttcacgt tcttttttcc cgatagtata
     5221 gagttctgca gtgaacatca ggttctgcca tgccgaaaga tcatcataga tattggatgt
     5281 ttcatgcacg atccccatct gtttgcgggc tgcggtcgtt tcccgtacga tatcttttcc
     5341 aaagattttg gcggtccctg cagtgggcgg gctgataccg gtcaggagac gtatggttgt
     5401 ggtctttcca gcaccgtttg ggccgaggaa accgaatgtc tcaccttcgg caacggaaaa
     5461 actgatatcg ttcaatgcga cgagtgcccc gaaatgcttc gaaagatgat caacctcgat
     5521 cgcgttcata ctatcttggt attaaccggt attggtttta aacaatcctt aaagactaaa
     5581 attgtggcaa catcatggga cacggtggat gtaaagaaag gacaacccga ccaaccctct
     5641 ttttttttga gaacgattgt gccgcacgaa tgtcggggat tattaaaaaa ggtaatcctg
     5701 agaggtgatc ccgctatacg tacgaccgga taacaatcac cggccgtgtt gcgttcatga
     5761 ccacatcgtg caccatgctg ccaaaaaaga agtcatgaag acatccctgt gctgcagatc
     5821 gcatccagat gagtgagacg tcctcagcct gcgcaacacg ggtgatctcg attacgggtt
     5881 tcccggtgac gacaatagtc ctggcattga taccctgtgc ggatagccgg tcgcagaccg
     5941 catggatccg ctgttctgcg gtggtaaggg cgagcgtgtg atcacccttt tcttcacctt
     6001 tctgtacaac gtgcaggaga atgatctccc ccataccctt gatagtactg acaagagcga
     6061 ctgttttttc agagaattcc gagaaattcg tcgggcagag gacccgggag aagatcatcg
     6121 gacagaactt ttcatatgtc ttcccggtca gcgatccgac gagggtattt ggcataatca
     6181 tgacattgat tttactgatg cggcagagca ccttcgaaga tacgcaattg aggaggacac
     6241 ccgcgatgat gcccttaatg tgtgcgtgga tcacaatcag ccctgcatga tgttcttctg
     6301 ctttttccag gattgcaccg gtgatatctg ttgctatggt ctcttcgagt gtcacacgga
     6361 tagccgggtt gagtgatgcg atataggctt tggcttcatg aaggtacact tccgtggtct
     6421 cgagttccag gttttcgatg gtttccacac ccatgggaat cgcgtactgt tgtatgacgt
     6481 ggaggaggat tatttcccgg ataccgggaa tactggtaat gcagttaagt tccgtcctcg
     6541 caggttttga aaaatctgtc gggaaaataa ttttttcaaa catacaccct cactatgcga
     6601 ttctagtcag atgcccggga gagtcaggtt attaaagaca ttacatattg ctcacattat
     6661 ataattatat gcaacaaaaa atcacccggc aaacaaaaaa gacgatatcc ctcgcaacct
     6721 ttatgatcgt cccggcaggc tgttggccat agtaggtaat cagttgattt ttactcctat
     6781 tccttcaaac caccgtgatt caaaatttcc cgagtaatcg tattcatagc cgatttttga
     6841 aaaaaaagct tcgaactctt tctgggcaag ggaactttca tccgcagtat tttttgtccg
     6901 gtaaaaatgt gccaggtaga caaaacttac cccgtgatca gggaaatctt cccgttggag
     6961 ggaatgaacc atcgggtact gctcaagata tgcctcccag ccgggatagt tgcatttcac
     7021 ctcgggtgga atgtcttcag gtccgtagat atccgctccc gtgatttttt gtgcagggac
     7081 ctggtgcttt tttattcgtg tatgcacttt ttccctccct ttctgatcat gattacatcc
     7141 cggatgagct gcggggtctg tatcctccgg tgccatatgg ctattaaacc agtcacaagt
     7201 attaaaaatt tgttataaaa aaaactcttc gggtcggatc gcatcatttt tcaccggttt
     7261 tcgggaattt tttatgtgcc ctgaaatacc ggtcatcact acagacagtt cgtacctcct
     7321 tgtgatcagc cggataccgc aaactcccgt ttacccgctg caaaaatttt atattccgga
     7381 aacaagagtg atttttaatc agtatggggt gatactgatg gtaagaggtg aacgggaatc
     7441 agaccgcagg ggcggcgagg gatacatggg ccggggtatg cggggcagta acgatgaccg
     7501 ttgggatccg ggggacctgg agcgggaccc ttaccgtgat ctcaaagatg ggtttgaaga
     7561 tctgacggat cccctgaatg atgagtatat ctggcccagc acagaagaag aagaggacga
     7621 ctaaaagaga ccaggacttc cctggaattt ttttatccgg cgcgtaccat gtcacgtgta
     7681 ccggtctggt tttgtctttt tgcctgaccc ggggagcagg cggtttatgc ctacggacca
     7741 ggtctcaata cgaattccgc atgcatgatt ccgggatcca tcacgacaac taaataacca
     7801 ttaggttcac atacgttctt ttaaggatga ggaattacca tgagatggag agatggacga
     7861 cgaagtgata atgtcgaaga tcaacgggga tttggcgtta aacagggggc aatcggtggc
     7921 ggtatcggaa ttctggccct tcttgttatc ggtcttctga ccggtgctga tccggtttca
     7981 ctgataagtg caatcgttac ccaaagcggt tccctggaca gcactccggt agtactgacc
     8041 gcagccgaga atgaatcggc agattttgtg tccgttgtac tggccgatac agaagatacc
     8101 tggcagtcga ttttccagca aaatggccag gtgtaccggc aaccaaaatt agtcctgttc
     8161 aaaggcagcg tggaatccgc atgtggaaat gcccaggccg ccatggggcc attttattgt
     8221 ccgggtgacg agaaagtgta tattgacctg gatttttata atgaattaaa aaaccagctg
     8281 ggagcccccg gcgattttgc ccaggcgtat gtcatcgccc atgaagtggg tcaccatgtg
     8341 cagaaacttg atgggacctc tgcagaggta caaaggctcg aaagccagag tgacaaggtc
     8401 acagccaaca aatattcagt catgctggaa ttacaggccg attgttatgc aggaatctgg
     8461 gcgtatcatg ctgacaaaca acgtcagatg attgaagccg gtgatatcgg ggaagccatg
     8521 aatgccgcgg cgaagatcgg ggacgatgcc ctgcagaaac agtcacaggg ctatgtagtg
     8581 cctgattcct tcacgcacgg cacgtccgcg caacgcatga aatggtttaa tgccggttat
     8641 gtaagcggcg gtgtcggctc gtgcgacacc ttcaatgcgg ctgcactctg ataaccctga
     8701 ccattccgcc cagggataaa aaagactgaa acagcaaacc ggtaatggtc ggaaaaaagt
     8761 gaacgaggac tcaaaaaccc cttacaggtt atttgccgaa atagtccacg acttttttta
     8821 agttgtcacg gatcggcagg tcctgggggc atttctcctc acattctccg cattcctggc
     8881 agagcgacgc gtaatgggga ataccgtcaa agaaacctcc aaggaacatg gtgtagatct
     8941 gctttgtttg ttccgcgtct tcgtacatgt gcccgcggtt gtaatattca aagcactccg
     9001 ggatgctcac gccatgtgga cacggggtgc agtacctgca accggtacac gggatgagga
     9061 cccgcttctc cagttctttt ttcacttttt taaagaggga gagttctgtc tttgtaagcg
     9121 agttcgcaag accgctttgt gcggaa
//
LOCUS       DASRLV010000033         5985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_496313,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000033 DASRLV010000000
VERSION     DASRLV010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496313"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..346)
                     /locus_tag="VFC43_01295"
     CDS             complement(137..346)
                     /locus_tag="VFC43_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29858.1"
                     /translation="MAKKTKPLVKEESISSVEEEMIESVKKDTIAPGKEGEGFDAIRI
                     LMAIGVLFCVIFLVFFILRYVLHMI"
     gene            371..607
                     /locus_tag="VFC43_01300"
     CDS             371..607
                     /locus_tag="VFC43_01300"
                     /inference="COORDINATES: protein motif:HMM:NF016831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZK29859.1"
                     /translation="MNHFYKAFLLVYGEIRQSSGSNYREYRPKSLSSGYYEYPRKISA
                     TELAEKLGYSKSTTIEYLRKAENKIITTVYTGEL"
     gene            complement(738..2048)
                     /locus_tag="VFC43_01305"
     CDS             complement(738..2048)
                     /locus_tag="VFC43_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HZK29860.1"
                     /translation="MSYLLEIVIILILIVINGIFAMAEFAIVSVKKSRLQQRADTGDR
                     GAAAALEIVNEPTQFLSTVQIGITLVGIFAGAFGGATVARELAVYFTEFPALAPYSDL
                     LSIVIVVLVITYLTLIFGELVPKRLALNNAENIASAVAKPMRLLSVITAPLVIILSHS
                     TEAMLRVLQFRKITEPPVTEEEIKIMLAESTEAGVFEKAELSMVEGVFDLGDRRVESL
                     MTHRSDVIALDLNDPVDENLRKMTQSGRSNFPVFEGNLDTIVGMVSVKDLLARMVDGG
                     TPDIKASVTKPLFAPQALPVLKLLESFKGTGVHIALVTDEFGSIQGVITLHDILEAIV
                     GNVRTLGEPVEIPVVVREDGSWLISGDTPVEDIRKILSVDSFPGEEQGRYRTIAGLIL
                     FILERIPKTGDHFEAEGLRYEVVDMDGNRIDKVLVTRVPTEPPA"
     gene            complement(3942..4238)
                     /locus_tag="VFC43_01310"
     CDS             complement(3942..4238)
                     /locus_tag="VFC43_01310"
                     /inference="COORDINATES: protein motif:HMM:NF028226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HZK29861.1"
                     /translation="MLPRCDHASSYAGFHDLKTRRSGPEIFIEFHLVVPGTVSNFPYS
                     GVVTLSRIKPSRITKTFGNNQFDREGRIIISEYPSFVLYNCYFPTGASGKKTKI"
     gene            4333..5220
                     /locus_tag="VFC43_01315"
     CDS             4333..5220
                     /locus_tag="VFC43_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HZK29862.1"
                     /translation="MLRKLRANLYATMEVPAIEDNQGGYYEVLMMSLIFANAFAMIVA
                     TVPSIKLNYDWFLMPFEHISIIIFSLEYLLLLWVCTENPAYQDPVYGRIRYAMTPIAL
                     INLISVIPAFIPVLEPYDLRVLRMFRLFRFFRILKLTKYSDSIQTLFKALDAKKEQLF
                     MTLVIFILLVVMASVFLFYAENGANESAAFNDIPSTIWYSMVTLNPMSNEEGYPVTVA
                     GKLIAAGLALLEIGIFAIPAGIMASAFEEQYNEKQKDKDEKPTVCEEEFTRKDGPPRC
                     PHCGKSYTDEGGGTLPDNN"
     gene            5683..5853
                     /locus_tag="VFC43_01320"
     CDS             5683..5853
                     /locus_tag="VFC43_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29863.1"
                     /translation="MFTSAKRPTYAYIAITSLCNSRCRYCDSWKNKGESEPDTGEWKK
                     IIDELVNLGIVT"
BASE COUNT         1625 a         1424 c         1292 g         1644 t
ORIGIN      
        1 attatcctgt atttttgagt gtaagaacga aaatagttac gctttctttg cttttaaaaa
       61 aaatgtaaga attcagaatt caggagtgac tgcattctga atcgcatggg ttgcataaaa
      121 atggcaattt gcggaattat atcatatgta acacatatcg gagaataaaa aagacgagga
      181 aaataacgca gaataatacc ccaattgcca taagaatgcg gatagcatca aaaccctcac
      241 cttctttacc gggtgcgata gtatcttttt taactgattc tatcatctct tcttcaaccg
      301 atgatataga ttcttcttta actaagggct tcgttttttt tgccatgact atttcaccgt
      361 gcatatctct atgaatcact tctacaaagc atttctcctc gtttatgggg aaatacgcca
      421 gtcatccggt tccaactacc gagagtatcg accaaaatct ctcagttccg gctattatga
      481 gtacccgaga aagatcagtg caacggagct cgcagaaaag ctcggctaca gcaagtccac
      541 tacaatcgag tacttacgga aagcggaaaa caagatcatc acaacggtct acacggggga
      601 gctctgatct ggtaaactgc agagatcaag atcggtcgag actgttcgtc actgttgatc
      661 gtactcatgc cgaactcctg gtgcctcgta aatatcacta tttcccgctg ttgcagaccc
      721 ggtgaatagg ggagcgttta agcaggaggt tccgtgggca ctcgcgtgac cagcaccttg
      781 tcgatacggt tgccatccat atctacaacc tcatagcgca gtccttcggc ttcgaaatga
      841 tcaccagttt ttggtatccg ctcgaggatg aacaggatca gcccggcaat ggtccggtaa
      901 cgtccctgtt cctctcccgg aaaggaatcc acggataaga ttttcctgat atcttcaacg
      961 ggtgtatccc cgctgatcag ccatgagcca tcctccctta caacaacagg aatctccacc
     1021 ggctctccga gagtacggac attgccgaca atcgcttcaa ggatatcgtg cagggttatg
     1081 acaccctgaa tgcttccaaa ctcgtcagtg accagtgcaa tatgcacccc ggttcccttg
     1141 aatgattcga ggagtttcag gaccggaagt gcttgggggg caaagagcgg tttcgtgaca
     1201 gaggctttga tatctggggt gccgccatcc accatcctcg ccagaagatc tttaacggat
     1261 accataccga caatagtgtc caaatttccc tcaaataccg gaaaattgga tcttccactt
     1321 tgggtcattt tccgcaggtt ctcatcaacg ggatcattca gatcgagcgc tatgacatca
     1381 gaccggtgtg tcatcaggga ttcgacgcgg cggtcaccaa ggtcaaacac accttccacc
     1441 atgctcagtt cggccttctc gaatacaccg gcttcggtac tttccgcaag cataatctta
     1501 atttcctctt ctgttaccgg aggctcggtg attttgcgga actgcaggac ccggagcatg
     1561 gcttcagtcg aatgactgag aataatcacc aacggcgctg ttatgacaga aagaagccgc
     1621 atcggttttg cgacggcgga ggcaatgttt tctgcattat tgagcgcaag gcgtttgggg
     1681 acaagttctc caaagattaa agtcagatag gtgattacca gaaccacaat aacgatgctc
     1741 aataaatcgc tgtacggcgc cagagcaggg aattctgtaa aataaactgc gagttctcgt
     1801 gctaccgtgg ctcctccgaa cgcaccggca aagataccca caagagtgat cccgatctgt
     1861 actgtcgaaa ggaattgtgt gggttcattc acgatttcaa gtgcagctgc tgccccccta
     1921 tcccctgtat ctgcccgctg ctgaaggcgg gattttttaa ccgaaacgat ggcaaattct
     1981 gccatggcaa atatgccatt gataacgatc agaatgagaa taataacaat ctcaaggaga
     2041 taagacattt tgcagaagta ttgtgcgcac ggcaattaaa actgtttaaa gaaatgagga
     2101 ggattttgct accggtaagt cagaaaacgc aaaaaatcgg gttataccat ggtaaggata
     2161 gtattctctt tgataatggt tgggggtgat atcctgcgga tggtcaggat tgtacctgac
     2221 ttgggtgtga gatgatctcc tcctcatttc cctccatcga ggatacccca tatatgaggg
     2281 tccgaaccct gggggcaaat aagcccattt caggctccgt ttccattcct catataccgg
     2341 tcaataccgg taaaccacgt aaaatcctta ttttctgttc ctggggagaa tacgcttaaa
     2401 tttcactctg tttgccaaaa tacggcagat ttgcacctct ccaaatggtc gttaaagccc
     2461 tttaaatcaa ggtgaatttc gcctcttaat ttaccctatc caccctctgg aaatgccaac
     2521 agaacacgaa tatgaaagtc attttttctg acactaatcg gaaatgttcg ggaaaacaga
     2581 gattttttaa aaacttgaga aaaaagtgga agttaaaaaa attatatccc ggcaatccgg
     2641 agcgtcatcc caagcagaat tccgaacata gatgtggatg ctgataacat ccctaaaaca
     2701 aaatatccga ttataatacc aattttaatc ggtatgtagg tttggttgag attaagcctt
     2761 tgtaccttgc gaaaggattt tgtttctggc gaaacctgcg gtttattttt ctttatcctg
     2821 atcattgcgg atcaccctat gacaagattt tccctcacta gttctagagt ggaggctgtt
     2881 tttatttttt tgctgacgct atcaattgcc gtacattatt gataattact ataaataatc
     2941 tatttttatt caaactaaaa ttttgaaaat ttcgattttt ttaaagaata tctactttca
     3001 ttcacttttt gcgattacac aagaatggta gtatattgga tcgttcccga ctggaaagat
     3061 cttgatatcc tgcgaagagc aggcagacat atgcatatcg ccaagatcct tttaccaaca
     3121 tgaagatcgg tcgagactgt tcgtctccgc tgcattccgg ctcgcttcgc tcccctccat
     3181 tccgctccac tcacagactc tcccttgctt tttcacgttg tattactgag acgtagtacg
     3241 caccccttca ctcgcttcgc tcatttcggg cttgctgccc cggcccgctc ctcacaccat
     3301 gcttcgcatc gtgttcatcg ctgttgatcg tactcatgta taacgctcag tgactcattc
     3361 gcgctcaacc tctcgctgga taaggatctg gttcgggtca cttcggatat ttactaaata
     3421 atctcgcgat atcgtcaatg atcgagaata tttttttttg acaatctcga attctgcaca
     3481 gaacctgtat tgtcctggcc gcatatgctt catctgtaat gggttcgtac tgtttgctga
     3541 ttcaggatct ctgatctcga ggaaaatggg acagtgatca ccctttacga actgtggtcg
     3601 atgcgaacag gaccggaaaa ggcctttaat gcccggggat atcagaaaat aatcgatccg
     3661 ccaaccccat ccctttgttt tgaaatccgt gctgctccac caggtatagt gaccaccctc
     3721 gtcgttattt agccggaatg catcaatcag gtcaagcgag aacaatttct agatatcctc
     3781 gcagtcttcc ggcaaaaatc ccgggccaat ctttttgcgc agcacatcta caacatcccg
     3841 gtcgtctcga gcagtgttga aatcaccaca aagtataact tccttaccgg aggctaacgt
     3901 atttctaatc gattccgtgc aatcatggta gaacgctcgt ttcatatctt ggttttttta
     3961 cctgatgctc cggttgggaa ataacaattg taaaggacaa aggatggata ttcactgatt
     4021 ataattcgtc cctccctatc aaactggtta ttgccgaatg tctttgttat ccgggatggt
     4081 tttatccggg agagtgttac aacgccgctg tagggaaaat tagagaccgt accgggcaca
     4141 acaaggtgaa attcgataaa gatttccgga cctgatcgtc gtgtcttcag gtcatgaaac
     4201 ccggcataac tgcttgcatg atcgcatcgt gggaggatcc acgttagtca tcagtaaatc
     4261 tcggcatttt ggattcgtca atcaaaccaa aaccctcaat aaagaagagt tcttgataga
     4321 gacacgtata caatgctccg gaaactacga gccaatctct acgctacgat ggaggtaccg
     4381 gccattgagg ataaccaggg agggtactac gaagtactga tgatgagcct gatttttgcc
     4441 aatgcctttg ccatgattgt ggcaacggtc ccatccatca agctaaacta tgactggttc
     4501 ctgatgccat tcgaacatat ttccatcatc attttttcgc tggaatatct cctcctgctc
     4561 tgggtctgta ctgaaaaccc ggcataccag gacccggtgt acggtcggat ccggtatgcg
     4621 atgacaccaa tcgccctgat taacctgata tcggtaattc ccgccttcat ccccgtcctc
     4681 gaaccgtatg acctcagagt cctcagaatg ttccggctgt tccggttctt ccggatcctg
     4741 aaactgacaa aatattcgga ctcaatacaa acgctcttca aggcactcga tgccaagaaa
     4801 gaacagctct tcatgaccct cgtcattttt atcctccttg ttgtcatggc atcggtcttc
     4861 ctgttctatg cagagaacgg tgccaatgag agtgcagcat tcaacgatat tcccagtacg
     4921 atctggtata gcatggtgac gctgaatccg atgagtaatg aagagggata tccggtcacg
     4981 gtggccggaa aactgattgc tgcagggctg gcactcctcg agatcggtat atttgcaatc
     5041 cctgcgggaa ttatggcatc cgcttttgaa gagcagtata atgaaaaaca gaaagacaag
     5101 gatgagaaac ctactgtctg tgaagaggaa ttcaccagaa aggacggacc tcctcgctgt
     5161 ccccattgcg ggaaatccta tacagatgag ggagggggga cccttcctga taataattga
     5221 actgtttttc aggcgggcaa tcgatcttct cacccggcac gcctttcaga agagactgat
     5281 ctgtccagaa aaagagcatc ctgtcatcca tggagaatca ggtaccataa aatttacgtc
     5341 cattctccga gatgaccata aaaaatgcac acactaaaaa tatgctttta aaaaaataag
     5401 gtacgccaac ctcgttctta gtcattaaat gcctaacgct cagtgactcg ttcgcccggc
     5461 ctctcgctga agcgttcggt aacagggaca agctactttg tctcctgcgt tttgttcagt
     5521 gttcgggaaa gatcggatac cccggtaacc agctttatat atcaggagtg acatgatttg
     5581 tccggtacag gtgccggatt taccgcatta aaaagccact tacgtgatac tatgaacaaa
     5641 tcagccggct tgagtttttt cagaaaccac ctgtctaatc gtgtgtttac ttctgccaag
     5701 cgcccgacct atgcgtatat cgccattaca tctttgtgca attcccggtg caggtattgt
     5761 gactcgtgga aaaataaagg agagagcgag ccggataccg gcgaatggaa aaaaattatc
     5821 gatgaattgg taaatctcgg tatcgtgacg tgaagaagac gaaaaatctt atttcaccag
     5881 gtttatttca tccctttctt actactattt ttaaatattt tttttcctgg actcccggcc
     5941 aggcgcgaag acatagtgaa aaagtaagat cgataacttc tatat
//
LOCUS       DASRLV010000034         2139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_820753,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000034 DASRLV010000000
VERSION     DASRLV010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820753"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            384..1202
                     /locus_tag="VFC43_01325"
     CDS             384..1202
                     /locus_tag="VFC43_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HZK29864.1"
                     /translation="MKSISLRFRIYLAILAAVLLIGVTGLIVIEGYSLLDAFYFIIVT
                     IATVGYGDLHPLTPLGKILVIGIILAGVGCFVGVVANAIEYMIDMRERAQRIEKLNMI
                     IGVFFSEVGTKLLKKFSAIDPDIQGIRTTLIVSNNWSDEDFFKANALLKVHSFRLDCR
                     ALSLEELHAFLSENKRLMLTLLENPQLFEHDRFTDLIHAVFHLSEELIARGEFGGLPK
                     SDYDHISVDMNRVYSQLVIEWLVYMQHLKKKYPYLFSLAMRTNPFDTNASPIVG"
     gene            complement(1344..2093)
                     /locus_tag="VFC43_01330"
     CDS             complement(1344..2093)
                     /locus_tag="VFC43_01330"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HZK29865.1"
                     /translation="MIEVHPAAIISPRWIGVDLNTTGHVAVAADPISGKVMKLGKNIH
                     YNHSHSLKNCTKLWKEEKLWKLKKFKTRERKNFKTAINTISRQIVSFAESLGSGIKFE
                     KLFSASYSRPETLEGPYNFSFENGSFYSLQRLVEKRANERGIEVIYVNPAYTSKRCSR
                     CGNYGRRSRKRFACPHCGHSAHADVNAAFNIATTTRRLAFIDTRAGLEETARLSKKQL
                     RKSVREQPIPDISFRIVTAFTWPENVLAVLE"
BASE COUNT          532 a          484 c          482 g          641 t
ORIGIN      
        1 cctgaacccg gatcaatttc caattcgcaa ggatctgatc cctcccggtg tttatgatca
       61 ccgtatgtta tgtgagcatc acattgggtc agatgcggac attgcttaca atcggaaaag
      121 gttttaagga ccatgatagt acttcgttgc ctataccacg gactcaatca atataaaatg
      181 gggctttctt ttcaaaaatt gcaacctttt tgaaccctca gaggtggagc agctggcata
      241 aagctgaatt cgctcatcag gatggcatat acaattcttt tttgtttcat catcacgcaa
      301 aaggttccgg ttcccatcaa taatggtctt gtccttctct aatcgtatcc cttttataga
      361 attatgctat agaacagtaa ataatgaaat ccatcagcct gagattccgt atctaccttg
      421 caatcctcgc agcggttctc ctgatcggtg taactggatt gatagtcatc gagggctatt
      481 ccctgcttga tgcgttttat tttattatcg taacgattgc aaccgtgggg tatggagacc
      541 ttcacccgct tactcctctc gggaagatac tggttattgg aattatcctt gccggtgttg
      601 gctgttttgt cggtgttgtg gcaaacgcca ttgaatacat gatcgacatg cgggaacgtg
      661 cccagaggat cgagaagctg aacatgatca tcggagtttt tttttctgag gtggggacaa
      721 aactcttaaa aaagttcagt gccatagatc cggatattca gggaatacgc accaccctga
      781 tagtatcgaa taactggtct gatgaggact tttttaaagc caacgccctg ctcaaagttc
      841 attcattccg gctggactgc cgggcacttt ctttagagga attgcatgct tttctctccg
      901 aaaataagag actgatgctc acccttcttg aaaatcccca gttgtttgag catgacaggt
      961 tcacggatct gatccatgcc gtatttcatt tatccgaaga actgatcgcc cgtggggagt
     1021 ttggcggtct cccgaagtca gattatgacc atatttccgt ggatatgaac cgtgtataca
     1081 gccagcttgt gattgagtgg ctcgtgtata tgcagcacct taaaaagaaa tacccatatc
     1141 tcttttcgct ggcaatgagg acaaacccgt tcgatacaaa tgcatcaccg attgtggggt
     1201 gatgcggtat tttacccgtc actcagatct tttgaatggt tgccattgat acatgggtag
     1261 attagattcc gaacagaatt ttaccgtcat cggcatagtc catgtgatct gtgggcaatt
     1321 ttttaccggg taaatttttt gaattattcc aacacggcga gaacattttc aggccaggta
     1381 aatgctgtca caatcctgaa tgaaatatcc ggtatgggtt gctccctgac ggactttctt
     1441 aactgttttt ttgataaacg ggcagtttcc tcaagtccgg cccgggtatc aataaaggcc
     1501 agtctcctcg tggttgttgc aatattaaac gcagcattca cgtcggcatg tgcactatgg
     1561 ccacagtgag ggcatgcgaa tcgcttgcgc gacctccttc cataattacc acaccggctg
     1621 cagcgtttcg acgtgtaagc agggtttaca taaataacct cgattccccg ctcgttcgcc
     1681 cgtttctcca ccagacgctg gagggagtaa aacgatccat tctcaaagga gaaattatac
     1741 ggtccctcga gcgtttcggg tcgggagtaa cttgcagaaa agagcttttc aaatttgata
     1801 cccgaaccga gcgactctgc aaaggagacg atctgccggg agatagtatt gatggcagtt
     1861 ttaaaatttt tccgttcgcg ggttttgaac tttttaagtt tccagagttt ttcttctttc
     1921 cataatttcg tgcagttttt caatgaatga ctgtgattgt agtgaatatt tttccccagt
     1981 ttcattactt tcccgctgat cgggtcggct gcgaccgcca cgtggccggt tgtgttcaga
     2041 tcaacaccga tccagcgagg tgatataatt gcggctggat gaacttcgat caccttccca
     2101 tctttgataa ttcaactgct gtacgaaatc caatcaggg
//
LOCUS       DASRLV010000035         5846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_902634,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000035 DASRLV010000000
VERSION     DASRLV010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902634"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VFC43_01335"
     CDS             complement(<1..382)
                     /locus_tag="VFC43_01335"
                     /EC_number="5.4.99.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016993 - precorrin-8X methylmutase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HZK29866.1"
                     /translation="MSKELLRQGDITANTYIDPGAETREAYEISKKSRTLARSVIGNE
                     NREDRIRQRCSVAVGDFGMADLMRFSHNPVAATLSALLGGAPIITDIRMVQVGIQKKG
                     HTSEVLCALDFGAEIAHARVIPRAW"
     gene            complement(330..1112)
                     /gene="cobJ"
                     /locus_tag="VFC43_01340"
     CDS             complement(330..1112)
                     /gene="cobJ"
                     /locus_tag="VFC43_01340"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HZK29867.1"
                     /translation="MPSHIEPKGRLAIVGLGPGLLEYISPRALKVIACSDYVIGNALY
                     LDPLEPLLAGKNVIRSSMGKEVDRAKQAIDLAKSHSVAIVSGGDAGVYGMASIVLEVL
                     EHSGAPIDVEVIPGITAANAAASRVGSPLSGDFAVISLSDLLTPLEVIEQRLDAIFSV
                     GIPLVLYNPKSRGRPHNFALAIEHARRYLGAKTPVAIVKNALRDGEEVIITTLENVNS
                     VEHIIDMHTTVIVGGKESRIWRMGEDVKGIITPRGYHCKYVY"
     gene            complement(1096..1968)
                     /gene="cbiG"
                     /locus_tag="VFC43_01345"
     CDS             complement(1096..1968)
                     /gene="cbiG"
                     /locus_tag="VFC43_01345"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HZK29868.1"
                     /translation="MTDTIVIALPRFMDDAKKIADFLGADIREYTPDIFYEIFPSTRR
                     IVAVMSMGIVVRRIAPLIHDKWNDPAVVVVSPDITYAVPVLGGHHGANELAKELSFLG
                     IQPVITTATEATGRDSVELIAQRNGCDIVNRDSTRKVNAAMLETDVPVFAVKGPGIVI
                     AGPCVSILVKKGEFTVGVGCRKGVRSKEVVDAIRNALEESGVAESDVLVYATTRKKSG
                     EAGLSDAISALSGNLIFLDDDIINAQRGISPSRAVSIGLRGVAEPCALAVAKKKQLVM
                     EKKVFGRVTVAIAH"
     gene            complement(1965..2684)
                     /gene="cobM"
                     /locus_tag="VFC43_01350"
     CDS             complement(1965..2684)
                     /gene="cobM"
                     /locus_tag="VFC43_01350"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HZK29869.1"
                     /translation="MGAKVWFVGAGPGNPELITVKGLSLLRDADVLIYAGSLVNPELI
                     AQSPAPIKLDSWGMKREEIIAAMEEAARSGKTVVRLHSGDPSLYGAIIEQIEELEKLN
                     IPVEIVPGVSSLFAAAAALESQLTLRGVSESLIVTRPAGKTLKHDQIRELSTLGVTMV
                     IFLGTEKLDEITKKLACPKDTPAAVVYHASWPDQKIVRGTVSDIAQKTQDEGIDHTAL
                     LIIGGVVDPGRKGYTRSYLYS"
     gene            complement(2687..3292)
                     /locus_tag="VFC43_01355"
     CDS             complement(2687..3292)
                     /locus_tag="VFC43_01355"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HZK29870.1"
                     /translation="MLIGLGLGPGNPELLTLRAVRLLKEADRVFVPGRIAKDLVAPYR
                     EATVLDFPMTDDESKIRQCLLENADKIAPAAQDGLVVFGILGDPNFFSTFSRLCSILS
                     EKYPSVKFRTEPGISSITAFAAAAGISVDDSFIVSDGQMPDSRILLKVRRPKEKADEL
                     RKQGYSKFILVERMYFADMKVYRNNELPDESDYLSVMYARR"
     gene            complement(3286..3825)
                     /locus_tag="VFC43_01360"
     CDS             complement(3286..3825)
                     /locus_tag="VFC43_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZK29871.1"
                     /translation="MSGIKLAGGPTQDEIMAVSLFKLGLRNTDTVLEIGCGTGKISVA
                     IAQQAKKIFSIDKRPEAITIARESAQKVPVSNIEFFCTDAVEFLESERLFDCAFVGGT
                     QQLARILPMLAKKVRRTIVINAVLLSTLDQAVKSMKQLGIFCEVIQVQVTRSHEIAGS
                     IMFKPIDPVYVIVGTGTAC"
     gene            complement(4450..5190)
                     /locus_tag="VFC43_01365"
     CDS             complement(4450..5190)
                     /locus_tag="VFC43_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29872.1"
                     /translation="MKNHEWNRMYVSIIFAGFFSAMCVAAFTFIDGMAGGYAIALVSF
                     FIAVSGIAVAGLFFHRARVMDEILNSTRILAHWVYSPDMAQESARREYGEYLERNRAM
                     FILIGGMLVVTALIFIIFVEEGGLITGVFLLAFTVVLFIISRITPGLELNRALKTPHE
                     AYIARNGIIYEGTVYPFQSFMMGMFDVSMQQGTGKKNAALIFSFTQLVGLYIIQAFDI
                     VVPIPDGELDTARGIVSSLGGEVEAEKK"
     gene            complement(5277..5780)
                     /locus_tag="VFC43_01370"
     CDS             complement(5277..5780)
                     /locus_tag="VFC43_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5804 family protein"
                     /protein_id="HZK29873.1"
                     /translation="MNILLIQRDGVDLHHTLFASETSRMALRFYHPKKKSCGLCITVS
                     TLGSALSLVSELRWYLRRYVQETLFEVKTGTYCTHTLAQDVYYERTSVLEPDWPFRRL
                     YGFRDGKLLTRVVMSPGSTIQEYHQEYAGINASIQCWCTEDEVEEGELLHEENGDSAG
                     ILQPEEE"
BASE COUNT         1511 a         1467 c         1284 g         1584 t
ORIGIN      
        1 cccatgcacg gggaattacc cgtgcatggg cgatctctgc accgaaatcg agtgcgcaca
       61 gaacctcgct ggtatgccct tttttctgga tgcctacctg aaccattcgg atatccgtaa
      121 taattggagc cccaccaaga agtgcactta gtgttgcagc aaccgggtta tgggagaaac
      181 gcatgaggtc agccatgcca aaatccccga cagcgacaga acatctctgc cgtatccggt
      241 cttccctgtt ttcgttgcca attaccgaac gggcgagcgt tcgcgatttt ttcgaaatct
      301 cgtacgcttc cctggtttct gcaccaggat caatatacgt atttgcagtg atatcccctt
      361 ggcgtaataa ttcctttgac atcttcaccc atcctccata tcctgctctc cttcccgcca
      421 acaattactg tcgtatgcat atctatgatg tgttcaaccg aattcacgtt ttcaagtgta
      481 gtgataataa cttcctcacc atcgcgcaaa gcatttttaa cgattgctac aggagttttt
      541 gcaccaagat atctgcgggc atgttcaatt gcaagcgcaa aattatgtgg tctgccccgg
      601 ctcttcgggt tgtagagtac aagaggtata cctactgaaa agattgcgtc gagccgctgt
      661 tcaattactt caagcggagt caggagatcc gacaaactta ttactgcaaa atcgcctgag
      721 agcggagatc cgacccttga tgcggcggca tttgccgcag ttatacccgg tattacttca
      781 acatcgatag gtgcaccgga gtgctcgagg acttctaaga caatgcttgc cataccatat
      841 acaccggcat ctccacctga gacgattgca actgaatggg attttgcaag gtctattgcc
      901 tgctttgccc gatccacttc ttttcccatc gagctccgta tcacattttt accagcaagc
      961 agtggttcaa ggggatcaag gtacaatgca ttacctataa cataatcaga acaggcaatc
     1021 actttgagcg cccggggaga tatatattcc agaagtcctg gccccagtcc gacaatagcg
     1081 agtcttcctt taggctcaat gtgcgatggc aaccgtaacc ctcccgaaca cttttttttc
     1141 catgacaagt tgctttttct ttgcaacggc aagcgcacat ggctcggcaa cgccacgaag
     1201 cccgatactt acagcccggg agggggaaat ccccctctgt gcgtttatta tatcatcgtc
     1261 taggaatatt aaatttccag atagcgcaga gattgcatca gaaagtccgg cttccccact
     1321 ttttttcctc gttgtcgcat agaccagcac atcactttct gcgactcccg attcttcaag
     1381 tgcattcctg atggcgtcta ctacttcttt ggatcgaact cccttacggc agccaacgcc
     1441 gactgtgaat tcgcccttct tcaccagaat tgacacgcag ggtcctgcta tgacgatacc
     1501 gggacctttc acggcaaata ccggtacatc tgtttccagc atggcggcat tcaccttcct
     1561 tgttgaatcc cggttgacaa tatcacaccc gtttcgttgt gcaatcaatt cgaccgagtc
     1621 cctgcctgtt gcttcagtgg ctgtcgtgat aaccggttgg atcccaagaa atgacaattc
     1681 ttttgccagt tcatttgcgc cgtgatgacc cccaagaacc ggaaccgcat aagtaatatc
     1741 cggacttacc actacaacag cgggatcatt ccatttatcg tgaataagtg gtgcgatcct
     1801 ccgcactaca attcccatag acattacggc aacaatacgt cgtgtactgg gaaaaatctc
     1861 ataaaaaata tccggggtat attcccggat gtcagcaccg agaaaatccg caattttttt
     1921 tgcgtcatcc atgaaacggg gaagtgcgat gactatagta tcggtcatga gtagagatag
     1981 gacctcgtat atcctttcct tccgggatct accacgccac ctatgataag aagagccgtg
     2041 tggtcgatcc cttcatcctg tgttttttgt gcaatatcag aaacagtacc acggacgatc
     2101 ttctgatcag gccaggaagc atgataaaca actgcagccg gggtatcttt cggacaggca
     2161 agtttttttg taatctcatc cagtttctca gtaccaagaa atataaccat ggtgactccg
     2221 agtgttgaga gttccctgat ctgatcgtgt tttagcgttt ttcctgccgg ccttgttaca
     2281 atcaggcttt cagaaacacc ccgcagcgta agttgtgatt ccagtgcagc tgctgctgcg
     2341 aagagcgatg agaccccggg gacaatctcc accgggatat tgagtttctc cagttcttcg
     2401 atttgctcga ttattgcccc atacagtgag ggatcgccag aatgaagcct gacaacggtt
     2461 tttcctgacc tggcagcttc ttccatagcc gcgataattt cctcgcgttt cataccccag
     2521 ctatcgagtt tgatcggagc aggggactgg gcaatgagtt caggattgac aagagatccc
     2581 gcatagataa gaacatcagc atccctgaga agcgataatc ccttgactgt aatcaattca
     2641 ggatttccgg ggccagctcc aacaaaccag acctttgctc ccatatttac cgcctcgcat
     2701 acatgacgct caggtaatcg ctttcgtctg gaagctcatt gttgcggtac actttcatgt
     2761 cggcaaagta catccgctcg accagaataa acttgctgta gccttgtttg cggagttcat
     2821 cagctttttc tttcggacga cggaccttca ggaggatcct cgaatcaggc atctgcccgt
     2881 cagatacgat aaagctgtca tcaacagaga ttccggcagc agccgcaaat gcagtaatag
     2941 agctgattcc cggttctgtc cggaacttta cggaagggta tttttctgac aggatactgc
     3001 ataaacggga gaatgtggag aaaaaattcg gatccccgag gattccaaaa accacgaggc
     3061 cgtcctgtgc ggcaggcgct attttgtcgg cattctccaa aagacactga cggattttcg
     3121 attcatcatc cgtcatcggg aagtcgagca cagtcgcctc acggtacgga gcgacaagat
     3181 cctttgcaat tcttcctggt acaaacaccc ggtcagcttc tttaagaagg cgtacagccc
     3241 gcagggtgag gagttcagga tttccggggc caagtccaag accaatcagc atgcagttcc
     3301 cgttccgacg attacatata ccggatcgat aggtttgaac atgatacttc ctgcaatctc
     3361 gtgcgatcgg gtaacctgaa cctgaatgac ttcacaaaat atccccagct gtttcatgga
     3421 ttttaccgcc tggtcaagag tactgaggag tactgcgttt atcacgattg ttctcctcac
     3481 tttttttgca agcattggaa gtatccgggc gagttgttgc gttccaccaa caaatgcgca
     3541 atcgaataat ctctcagact cgagaaattc aacagcatcg gtacaaaaaa attctatatt
     3601 gctgaccggg actttttgtg cagattcccg tgcgatggta attgcttctg gccttttgtc
     3661 aatagaaaaa attttttttg cctgttgtgc tatggcgact gagatttttc ccgtaccgca
     3721 ccctatttca agaaccgtgt cggtattccg gagtccaagc ttgaaaagcg aaactgccat
     3781 tatctcatcc tgagttggtc cgccagcaag ttttatcccg gacatcttct caaattttgt
     3841 tgtgttaagg tattaatcct tgttttaccg gagatggaat atcggatatg tctgaattaa
     3901 caataacttc acactacatt tcttgtgtta ttgaatattt tgtcaaagga aaatcaaata
     3961 aacttgaaat ttacttctca agcatgatct agaggtttct ataggtttag aaacggttgc
     4021 gaattgtatg ttgtcctgca tacaccgtta atcgaccaga agatcatcga tattacacct
     4081 attttcatca cttatactca aaatgagtaa aataagttct ttgcgggctc tgaattcctt
     4141 ctttatgcac acttgtagtc gagaaaaacc cgtaaaaccg tctttttcgg ttcgtgtgga
     4201 gaatatgcct tataattgct cagtttgtca aaatacggca gatttacacc tcatacaatg
     4261 tcttgtaata ccctttgaat cgagcttgat tttaccacat agtttaccct atccaacacc
     4321 cggaaaacct ggcagaacac caatgtgggg gtctttttct ctgacggagg attccaggga
     4381 ggtgtttggg cagttgcatg aggtaagata atcgtgaagc aggagagtat tgaatataaa
     4441 aaattccggt tattttttct ctgcttcaac ttctcctcca agtgaactga caatcccacg
     4501 tgccgtatca agctccccgt caggtatggg aaccacaata tcaaaggcct ggataatata
     4561 aagcccgacc agctgggtaa acgaaaagat gagcgccgcg tttttctttc ccgttccttg
     4621 ttgcatggaa acatcgaaca tgcccatcat aaaggattgg aacgggtaca ctgtaccctc
     4681 gtaaataatc ccgtttcttg cgatataggc ctcatggggc gttttgaggg cacggttcag
     4741 ttcgagcccg ggagttatcc ttgagataat gaaaagaacg accgtgaatg cgagcaggaa
     4801 gaccccggtg ataagacctc cttcctcaac aaagatgatg aatatcaggg ctgtgactac
     4861 cagcatcccc ccgatcagaa tgaacatcgc acggttccgt tcaagatact cgccgtactc
     4921 ccggcgggcg ctttcctgag ccatatcagg agaatacacc caatgggcga ggatccgggt
     4981 gctgtttagt atttcatcca tgacacgtgc ccggtgaaaa aacaatccgg caacggcaat
     5041 ccctgagaca gcgataaaaa aagataccag tgcgatggca taaccccctg ccattccatc
     5101 aatgaaggtg aaggcagcaa cacacattgc tgaaaaaaaa ccagcgaaaa tgatcgatac
     5161 atacatccgg ttccactcat gattcttcat attttatttc tcctggtagc ttgaccgttc
     5221 tggttggtgt ctcacaaaat tattagaaga tctttgtctg aaaggcctat gtatctttat
     5281 tcttcctcag gttgcaggat acctgctgaa tctccgttct cttcatgcaa caattcaccc
     5341 tcttctacct catcttcagt acaccagcac tggatggatg cattaatgcc ggcatattcc
     5401 tgatgatatt cctggatggt cgatcccggg gacatcacga ctctggtgag caatttcccg
     5461 tccctgaatc cgtataaacg acggaaaggc cagtcaggtt ccagaactga agtcctctcg
     5521 taatacacat cctgtgccag ggtgtgggtg cagtaagtcc ctgttttcac ttcaaataag
     5581 gtctcctgca cataacggcg gaggtaccag cggagttcag atacaagcga cagggcactc
     5641 cccagcgtgg atacggttat acagagcccg caggattttt ttttcgggtg ataaaaacgc
     5701 agcgccatcc ggcttgtctc ggatgcaaaa agagtatggt ggagatctac tccgtcccgc
     5761 tgaattaaaa gaatattcat ccgggtctcc tgaaaaatgg tattttatgg gtaccatgtc
     5821 cccaatcagg tatacatacg gttatc
//
LOCUS       DASRLV010000036         3942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_253254,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000036 DASRLV010000000
VERSION     DASRLV010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253254"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="VFC43_01375"
     CDS             complement(<1..1091)
                     /locus_tag="VFC43_01375"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZK29874.1"
                     /translation="MLSVLLIDDEPALLEVLTPFIERSGEMSVHTAQSALEALKILPE
                     KSFDAIIVDYDMPEINGIEFLKILRSKGDTTPVIIFTGVGHERTAIEAINYGANFFLK
                     KDGDPQVQFHALSTMIKSAIESTYLGKKLGTTRKIISDMIYFSTDPGFAIDREGKVVA
                     WNAAMEQLTDTLAGDMLGKGDYHYSEPFYGKKRKMLVNLIFEPDEELKSQKYMIVSRV
                     AKGPVVAVTKGVKKDGGEWTLWSKAMPIYDMQGNFIAVVGIVRDVTGTFKDIMIDDET
                     SDAGEQMTGAVTPQTSKPAGLFGKIFSSASGKATSYYKEGVLLYRNEKKYAEAVVAFN
                     KALEINDKLPYVWNELGLCYREMKDNNNAL"
     gene            1330..2541
                     /locus_tag="VFC43_01380"
     CDS             1330..2541
                     /locus_tag="VFC43_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-related McrA-glycine thioamidation protein"
                     /protein_id="HZK29875.1"
                     /translation="MQLKSCKKTYNLETQRAAPLEETLARIEPKVPAAGITRVADITN
                     LDRIGIPVFSCIRPTAEAGAITVYNGKGATVEESRISAIMEGLERYSSELHDRKTHLA
                     SYDEMLTHGRVLDPKDLILPEGADVDRLMPWVEAYDIANNRDIIVPAHAVFHPLPRNF
                     LPLFRTNTNGLASGNTMEEAIFHALSEVIERDAWSLVEASRDTGAAVENIDDPTSLAM
                     QKKFADAQVEVQVRDITSDIGIPTMAAVADDVLLKDPMLLTIGIGTHTSARIAVMRAL
                     TEVAQSRLTQIHGAREDTTIAELRKRMGYDRTKRINSYWFKDNGRVDYNQIASYDSDD
                     FLTDIKYIIEALRNKGLDRVIVVDLTREEIGIPVVRVIVPGLEVFAMDPERRGDRVNN
                     AKDHRLSRAKS"
     gene            2504..3157
                     /locus_tag="VFC43_01385"
     CDS             2504..3157
                     /locus_tag="VFC43_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfuA-related McrA-glycine thioamidation protein"
                     /protein_id="HZK29876.1"
                     /translation="MQKIIVFLGPSLDQAAAEKILAEQYFPPARRGDLLAAAEKGATI
                     IGLIDGVFHQESAVAHREILTAIKKGVKVVGASSMGALRAVEMDTLGMVGVGEIYRMY
                     KSGELESDDEVALVFDPSSGLALSEPLINIRFTLKRAMREGIITLAEHDLLLASARSL
                     FYPKRTYRAIVASTMDTIDEKIREQFLEWVSENTVDQKREDAVAALEYIRKIAKEKS"
     gene            complement(3198..3572)
                     /locus_tag="VFC43_01390"
     CDS             complement(3198..3572)
                     /locus_tag="VFC43_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZK29877.1"
                     /translation="MAKIAYFQIPADDVGRAKTFYQSLLGWKIEPDTALLDKSLEWQN
                     IITGEPQEGTMNSGGIYKQMMPGPIMNFVSVEDLDRVLSNVENLGGSIMMPKNEIKTV
                     GLVAVIQDTEGNVIGLWTPEGR"
BASE COUNT          990 a          999 c          950 g         1003 t
ORIGIN      
        1 agcgcattat tgttgtcctt catttcacgg tagcagagac caagttcatt ccatacatag
       61 ggaagtttat cattgatctc cagggcttta ttgaaagcaa ctaccgcctc ggcgtatttt
      121 ttttcattcc ggtaaagtaa aactccctct ttgtaatatg aggtagcttt gcccgatgcc
      181 gaactgaaaa ttttgccgaa caagcctgcc ggttttgacg tttgtggcgt tacagccccg
      241 gtcatttgtt caccagcgtc actggtttca tcatcgatca tgatatcctt gaatgtgcca
      301 gtgacatccc gcacaatccc gacaaccgcg atgaaattac cctgcatgtc atatatgggc
      361 attgcttttg accagagagt ccattctccc ccatcttttt ttactccttt tgttacggca
      421 acaaccggac cctttgcaac cctgctgact atcatgtatt tctgactctt cagctcctcg
      481 tccggttcaa aaatgaggtt tacgagcatt tttctctttt ttccataaaa tggttctgaa
      541 tagtgataat ctccttttcc cagcatatca ccagccaacg tatctgtcag ctgttccatt
      601 gccgcattcc atgctacaac cttgccttcg cgatcgatgg caaaaccggg atctgtggag
      661 aagtagatca tatcggatat gattttcctg gtcgtaccaa gttttttccc aagatatgtg
      721 ctttctatcg cgcttttgat catggtgctg agcgcatgga actgcacctg cgggtctcca
      781 tcttttttca aaaaaaagtt tgcaccgtaa tttattgctt caattgccgt gcgctcgtgt
      841 ccgacaccgg taaagataat taccggtgtg gtatcccctt tcgagcggag aatttttaaa
      901 aactcgatcc cgttgatctc cggcatatca tagtccacaa ttatcgcatc aaaggatttt
      961 tccggcagaa ttttgagtgc ctccagtgcg gattgtgcag tatgaacact catttcgccg
     1021 gatcgttcga tgaatggtgt gagtacttcc agcaatgcgg gttcatcgtc tatcaaaaga
     1081 actgaaagca aactaattcc ccctgcttca tagtcaataa cacccgtatg ataatataag
     1141 tataggggat ttttacgcca ttcccaatcc ccgactaaag aaatgaccgg atccgaaact
     1201 gtacccttac tacaaaccgg agtgtggatc cgatcctcaa atcaaagtgt ataaaccccg
     1261 gcacagaata tttttagtaa taatctgata cgggattccc ccgggtatat ccaggctacg
     1321 gtgacctcca tgcagttgaa atcctgcaaa aagacctata atctcgaaac acagcgtgct
     1381 gctccccttg aagagacact agcccgcatt gaaccgaaag tgcccgcagc gggaataacc
     1441 cgggtcgcgg atatcacaaa ccttgaccgc atcggaatac cggtattctc ctgcatccgc
     1501 ccgactgcag aagcaggtgc aatcaccgtg tataatggca aaggagcaac cgtcgaagaa
     1561 tcaaggatat cggcgataat ggaaggcctc gaacgatatt cttcggaact gcacgaccgg
     1621 aagacccacc tcgcatccta cgacgaaatg cttacccatg ggcgggttct tgaccccaag
     1681 gatctgatcc tccccgaggg agcagatgtg gaccgcctca tgccgtgggt ggaagcgtat
     1741 gatattgcca ataacaggga cattattgtc ccggcccatg ctgtgtttca cccgcttccc
     1801 cgtaattttc ttcccctctt ccgcacgaac accaatgggc tggcatccgg taacaccatg
     1861 gaggaagcca tcttccacgc actctccgag gtaattgaac gggatgcctg gtcgctggtc
     1921 gaagcatcgc gggataccgg cgcagctgtt gaaaacatcg atgacccgac cagtcttgca
     1981 atgcagaaga aatttgctga cgcacaggtc gaagtgcagg taagggatat cacgagtgat
     2041 atcgggatcc cgacgatggc cgcagtcgcc gatgatgtcc tgctcaaaga cccgatgctc
     2101 ctcaccatcg gcatcgggac ccataccagt gctcggatcg cagtgatgcg ggcgcttacc
     2161 gaagttgcac agagcaggct gacgcagatt cacggggcac gggaagatac caccattgcc
     2221 gaactccgta agaggatggg ctatgaccgg acaaagcgga taaactccta ctggttcaag
     2281 gataatggtc gggtcgatta taaccagatt gcttcgtatg acagcgatga tttcctcacc
     2341 gacattaagt acataatcga ggcactccgg aacaaggggc tcgaccgggt tatcgtggtc
     2401 gacctgaccc gcgaagagat cgggattccg gtcgttcggg ttatcgttcc ggggcttgaa
     2461 gtctttgcaa tggaccccga acgccgggga gaccgggtga ataatgcaaa agatcatcgt
     2521 ctttctcggg ccaagtcttg atcaggctgc ggcagagaag atccttgccg aacaatattt
     2581 tccccctgcc cgacggggag atcttctcgc agcagcagag aagggtgcga ccatcatcgg
     2641 actgatagac ggagtctttc accaggaaag tgctgttgcc caccgggaga tccttactgc
     2701 gattaaaaaa ggcgtaaagg tcgtgggtgc atcgagcatg ggagctctcc gggctgtaga
     2761 gatggacact ctgggtatgg tcggggtcgg ggagatctac cgcatgtata agagcggtga
     2821 acttgagtcc gatgatgagg tggcactcgt attcgatccc tcatccggat tggcactatc
     2881 cgagccgctg atcaatatcc ggttcaccct gaaacgggcg atgcgggaag ggattataac
     2941 gctcgcggaa cacgacttgc ttcttgcttc agcccgatcc cttttctatc cgaagagaac
     3001 ctaccgggct attgttgcca gtaccatgga tacaattgat gaaaagatac gggagcagtt
     3061 cctggaatgg gtcagtgaaa atactgtcga ccagaaacgg gaagatgcgg ttgctgctct
     3121 ggagtatatc aggaaaattg caaaagaaaa gtcataacag tgagaatccg gttcggtaaa
     3181 aaatgagatt tccctattca ccttccttct ggggtccaga gaccgatgac attaccctca
     3241 gtatcctgga tgaccgctac gagaccaacg gttttaatct catttttggg catcatgatg
     3301 ctgccgccga ggttctccac gttcgaaagg acccggtcga gatcttcaac agacacaaag
     3361 ttcatgatcg gacccggcat catctgtttg tagatacccc cgctgttcat tgtgccttcc
     3421 tgcggttcac cggttatgat attctgccat tcgagtgact tgtccagaag ggcagtgtcc
     3481 ggttcgatct tccagccaag cagcgactga tagaacgtct ttgccctgcc aacatcatca
     3541 gccggaatct ggaaatatgc aatttttgcc atggtccggt atagtgcatc cgccttttat
     3601 attattttct tgtagccggg aaaacctcac gtactcctga aaacggtaaa atccctatgg
     3661 acattctccc ataaaaaatg gagtggatat gctcatttcc ctgcaagaag cgacacgctg
     3721 gtccccgaaa tgtattaaaa gaaattatca tcctttgatg attctcagga gttttcctgc
     3781 ctctttaggg ataccaggaa gacggagatt ccaatagcaa ataacagcac acaaagaaac
     3841 agcggggctg tttgagtttt ttctggtttg ggaagattgt gcgttgtggg tatcgatgtg
     3901 gtcccctcag atgggtctgg ctcaagtacc gttgcatttc cc
//
LOCUS       DASRLV010000037         4290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_253384,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000037 DASRLV010000000
VERSION     DASRLV010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253384"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /gene="dapB"
                     /locus_tag="VFC43_01395"
     CDS             <1..591
                     /gene="dapB"
                     /locus_tag="VFC43_01395"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HZK29878.1"
                     /translation="RPDVLIDFTVASAAVGNVKMAARNKVALVVGTTGVTHEQRTEMD
                     TAIQGNVPAVISSNFSLGVNIFWQILRDTAKLLKDYDIEVIEAHHRNKKDAPSGTAKT
                     ILQILDEEVGSRKKMYGREGMMERSDEIGVHVIRGGDIVGDHKVMFSKNFETIELSHR
                     AYDRSVFATGALQAARWVVGKKPGIYGMSDVLNLKK"
     gene            699..872
                     /locus_tag="VFC43_01400"
     CDS             699..872
                     /locus_tag="VFC43_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZK29879.1"
                     /translation="MVAIVDTSKCTGCETCVNECPASAIAMDNEKAKVDKDMCVDCET
                     CVDVCPSEAIHME"
     gene            1142..2161
                     /gene="asd"
                     /locus_tag="VFC43_01405"
     CDS             1142..2161
                     /gene="asd"
                     /locus_tag="VFC43_01405"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HZK29880.1"
                     /translation="MINVGVLGATGAVGQRFVELLADHPWFNLSTLAASERSAGQPYG
                     KIVNWRLDTPFPEKIAKLKVTATSPKAMKGVDLVFSALPADIATRVENEFADAGIAVC
                     SNASSHRMEPDIPLVVPEVNPEHLGLIDVQRDSGRDGFIVTNPNCSTIMMVTALAPLR
                     KFKFSDVRVATMQAISGAGFAGVAAMAIYDNVIPYIGQEEEKMETETLKIMGSLKGKK
                     VTNATFKVSASCHRVPVIDGHTMAVWVDIKEPAEELKAAFKDYKPPIKGLPTQPEESI
                     HFFDEEKDRPQPRLDRMRGKGMTVSVGRLREGIRFIAMGHNTIRGAAGASVLNAELIT
                     KKKYL"
     gene            2176..2457
                     /gene="albA"
                     /locus_tag="VFC43_01410"
     CDS             2176..2457
                     /gene="albA"
                     /locus_tag="VFC43_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006325 - chromatin organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein Alba"
                     /protein_id="HZK29881.1"
                     /translation="MQQPRENTVFVGNKPVMNYVLAVITQFNNGADEVAIKARGKAIS
                     RAVDTAEIALNRFLEGVTKKEIITSTEIIDTDSGKTNVSSIEIVLANAK"
     gene            2560..3066
                     /locus_tag="VFC43_01415"
     CDS             2560..3066
                     /locus_tag="VFC43_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29882.1"
                     /translation="MRYYTVVLLLLLTISMAVPVMAANADRYSYITVRDIQIKLDNGS
                     AIIHVNYKVDEGTRFIFFLLGKQDLKNKLMKILNYEDARIKRIDLSSADFTVEDAAFS
                     YGNGIFWYPSHAFNIVVPELSVQSPQAIRNFTMTREFPSGMGYFATDSDTTPETIKKN
                     EISERSSR"
     gene            complement(3672..4088)
                     /locus_tag="VFC43_01420"
                     /pseudo
     CDS             complement(3672..4088)
                     /locus_tag="VFC43_01420"
                     /inference="COORDINATES: protein motif:HMM:NF016048.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF373 family protein"
BASE COUNT         1291 a          894 c         1015 g         1090 t
ORIGIN      
        1 agacctgatg tactcatcga tttcactgtt gcaagtgctg cagtcgggaa tgtaaaaatg
       61 gctgcccgga ataaagtggc actggtagtg ggcactaccg gagttacgca tgaacaacgg
      121 acagagatgg atacagctat ccagggaaat gttcctgcgg tcatctccag taacttttca
      181 ctgggtgtca atatattctg gcagatcctg cgggataccg caaagctctt aaaagattat
      241 gatatcgagg tgatcgaagc acatcaccgg aacaagaagg atgcccccag cggaactgca
      301 aagacgattc ttcagattct tgatgaagaa gtcgggtccc ggaaaaagat gtacggccgg
      361 gagggaatga tggaacgctc agacgagatt ggagtacacg tcatacgcgg gggagacatt
      421 gtcggtgatc acaaggttat gttttcgaag aattttgaaa ccatcgaact ctcccaccgg
      481 gcatatgaca ggtcagtttt tgcaacaggg gcactccaag cggcacgctg ggttgtggga
      541 aaaaagcccg gaatttatgg aatgagcgat gtgcttaacc tcaaaaaata atttttatgt
      601 gttcctctga tttttaaaat aattccgaaa atcatggaat gacagaatcg aaacctattt
      661 tttatctttt gttcaacata tacgatggag tgaacaattt ggttgcaatc gttgacacaa
      721 gtaaatgtac cggatgcgaa acctgcgtaa atgaatgtcc agcttcagca atcgctatgg
      781 acaatgaaaa ggcaaaagtt gacaaggata tgtgtgtaga ctgtgaaacc tgtgttgacg
      841 tctgcccgtc tgaagctatt cacatggaat aaggtgtgat tttttttttg gaaaaatttt
      901 ttttattgtt tttctggatt caatacgatc gaaaaaaatt gatctttttc ttcccaccat
      961 taaagatctc atacaggttt ttcaggatta atcctggtta ttactaagga atcattctgt
     1021 ggtaattttt cctgcaggag aaaatgtaaa aaacaacgtt tttattatca cagatgaaat
     1081 gggattttac agcccgccat atttcaacgg gaaaacttaa cacacacagg acctgacaca
     1141 catgatcaat gttggagtgc tcggagctac gggtgcggtt ggccagagat ttgttgaact
     1201 tttagcggat catccgtggt tcaatctttc aacgcttgcc gcctctgaac gcagtgcggg
     1261 acagccttat gggaagatag tgaactggcg cctggatacg ccatttcctg aaaagatcgc
     1321 aaaactgaaa gttacagcca catcgccaaa agcgatgaag ggcgtagatc tggtcttttc
     1381 tgcgttgccg gcggatatcg ccactcgtgt cgaaaatgaa tttgccgacg cggggatagc
     1441 cgtctgcagt aacgcgagtt cacatcgtat ggaaccagat ataccgcttg ttgtaccgga
     1501 ggtaaaccct gaacatcttg gccttattga tgtccagcgt gattccggca gagacgggtt
     1561 tatcgtgacc aaccccaatt gttccacgat tatgatggta actgcccttg cccccctgag
     1621 gaaattcaaa ttctccgatg tccgggttgc tacgatgcag gcgatctccg gtgcagggtt
     1681 tgccggcgtt gcggcgatgg caatctatga taatgtcatt ccgtatatcg gtcaggaaga
     1741 agagaagatg gagaccgaga ccctcaagat tatgggatca ctgaagggga aaaaagtgac
     1801 aaacgccaca tttaaggtga gtgccagctg tcaccgggtc ccggtaatcg atggtcacac
     1861 gatggcagta tgggttgata ttaaagaacc cgcagaagaa ctaaaagcgg ccttcaagga
     1921 ctataaaccc ccgataaaag gcctcccgac ccagcctgaa gaatccatac acttctttga
     1981 cgaggagaag gatcgtccac aaccgcggct ggaccggatg cgggggaagg gaatgaccgt
     2041 ttcggtaggc aggcttcggg agggtatccg cttcatcgca atgggacata atactattcg
     2101 tggagccgcc ggggcaagtg tactgaacgc agaactgata acaaagaaaa aatacctctg
     2161 accaggtggt ccataatgca gcagccaaga gagaacacag tattcgtagg aaacaagccg
     2221 gtcatgaatt acgtacttgc tgtcattaca cagtttaaca atggagcaga tgaggtcgca
     2281 ataaaggcgc ggggcaaggc aatttcccgt gcagtggata ccgcagagat tgcccttaac
     2341 cggttcttgg aaggagtaac aaaaaaagag ataatcactt cgaccgagat tatcgacacc
     2401 gattcgggaa agacgaacgt atcgtcgatt gaaattgtcc tggccaatgc caaataatct
     2461 tttttaaggg tatttcaggt acagatacgg gaaataccga taataatcag agattcttac
     2521 ccaaaacaat ataaacggca ttcacgaatg tactaaccaa tgcgatatta tacggtcgtg
     2581 cttctgctct tactgacgat cagcatggcg gttcccgtta tggctgcaaa tgcagatcgg
     2641 tatagctaca taactgtcag ggatatccag atcaagctcg acaatggtag tgctatcatt
     2701 catgtcaatt acaaagtcga tgaaggcaca cggttcatct tttttttgct gggtaaacag
     2761 gatcttaaaa acaaattaat gaaaatactc aattatgaag atgcccggat aaaacgaatt
     2821 gatctttcca gtgcagattt tacggttgaa gatgcggctt tttcctatgg gaacgggatt
     2881 ttctggtacc cctcgcatgc cttcaatatt gtggtccctg aactctccgt tcaatccccc
     2941 caggcaataa ggaactttac gatgaccaga gagttcccga gcgggatggg gtattttgct
     3001 accgattcag atacaactcc cgaaaccata aaaaagaatg agatttcaga aagaagttcg
     3061 cggtagatat cttttctttg ttcactccgt tgatcagatc aagggttgct taatcagggg
     3121 agttattttt ggaaaaaaaa cactgtcaac catgaaaaat cgcaataatg atagaaggga
     3181 cgcttcgcta caagtgaatc accttggcga tgttcatggt tcccggtctg gagcccgtcc
     3241 tcccatcagg gaggctatca caaaaaagta tacatatccc ccggatcggg ctccctggtt
     3301 ggtgacaaaa acacaccttc cgttgcgtat tattaaaaaa acacttgaac attttttctg
     3361 ctctggagaa actcccatga accgataacg ttcacttgca aacaatgaaa aaaattttct
     3421 ctataaaggg ccctgccgcc ggttttctct gagaaatcgc gggcaggaga tttcagtaat
     3481 taagccgctg caggagcgtc ccccattgcg atgaagaggt atttactgtt gccaacctca
     3541 gtttatctgt cttgaaccac gtcctccaac aggaactaat gagcgcaaag agggtttctg
     3601 cagataattt ttcatcgatt caccgctttg aaggaacgct ggcatccgtc ttaattaaac
     3661 gggaaaggcg atcacatgac ttcaataatt tcctgcttgc gaagctcggc aattttctta
     3721 ttgacaaaga actggatgat catgccaata attgcagaaa taataccaaa gaccgcaaaa
     3781 tagagtacct gaattgctac accgtctggt gtaatgggaa aattcgggat atcttttacc
     3841 ggaaggatag aaagacttgc gcagtagaga atgaacccga ttgccgtgat aaagaaggga
     3901 aagacgatca ctttgcccag gttctcacgt tcattcaggt acacgtcaat aataattcct
     3961 atagacatta ccaggccggc tgcaatcagc cagcctattc ctccaaaaat aaataacata
     4021 agataatgga ttattccaaa atccgccgag tagtatttca ataccgtcag aaaaccgttg
     4081 gtgttatgaa aaactgggag aaagaataaa aggaattatc gtaacggatg ttaaggatgg
     4141 caatgaaatc attgaaccgt tagaagatcg aattgtaggc cggtatacgg tagaagatat
     4201 tgttgatcct gtaactggtg aaatcattat agcagcaaat catgtgatta gccataaagg
     4261 aggagatcga atcattaaag ctggttttaa
//
LOCUS       DASRLV010000038         4372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_253513,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000038 DASRLV010000000
VERSION     DASRLV010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253513"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..3387)
                     /gene="carB"
                     /locus_tag="VFC43_01425"
     CDS             complement(217..3387)
                     /gene="carB"
                     /locus_tag="VFC43_01425"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HZK29883.1"
                     /translation="MPRRPDIKKVLMIGSGPIQIWQAAEFDFSGSQACRALREEGINV
                     VLVNSNPATIMTDPEMADEIYIEPLRADVIARIIEKEKPDGILSGMGGQTGLNLTVEL
                     AEMGALKGMEILGTPLEAIYKGEDREKFRDLMTEIGEPVPKSIIINSLSQIDEAIKTI
                     GLPAVVRPAYTLGGAGGGIGKTKEELVRIIELGLARSRIHQVLIEESVLGWKEIEFEV
                     MRDASDTCIIVCGMENVDPMGIHTGESVVVAPILTLRDDEFQMMRSAAIHIIRALDVQ
                     GGCNVQFAFLDGDYRVIEVNPRVSRSSALASKATGYPIARVAAKIAIGLRLDEIMNTV
                     TGCTPASFEPAIDYIVAKVPRWPFDKFKGADRTLSTSMKSTGEVMAIGRTVEEAFMKA
                     KRSIDTDVIPHTSPSEIRMILSRPTDERFHCLFDAFRQGFSIDEIVQLTSITPFFLEK
                     FKNIVDMEKHLVEHHQPEDILAAKKYGFPNTDLMKITGLSLEQVEEMAGLPSYKMVDT
                     CAAEFPATTPYFYSTYEQACEIIPNENKKILILGSGPIRIGQGIEFDYCTVHAVQALR
                     DEGVEVHIINNNPETVSTDFDTSDRLFFEPMQLEDVVNVLKTDYYYGVMVQFGGQNAV
                     NLAVPIEEEIKRLGLKTKILGTSPDAMDIAEDRDRFSRLLSSLNIPSPANSSAYSEGD
                     ARAMAARIGYPVLVRPSYVLGGRAMEIVHDERELETYMKEAVRVSKNHPVLIDSYLQN
                     AVELDVDAVCDGKEVLIGGIMEHIEQAGIHSGDSACVIPTQSLSPEILDTIRDYTKKI
                     ALGLGVVGLVNLQLAVKDNVVYILEANPRASRTVPFVSKATGIPVAKIAAKVMIGRKL
                     RDLGYKERVIHHVAVKEVLLPFNKLAGVDTMLGPEMKSTGEVMGIDYDFGLAFYKACI
                     SADNELPLKGNIFISVNIEQKEEAIPIARQLRDLGLILYGTEGTVDYLYEAGVEAHLV
                     RKVQEGSPNVLDMMRHGEIRLIINTPQDKQSRQDHYQIMRAAVDFGIPYITTLQAARA
                     AALAIDAIKREKVTLEPISHYLRGTL"
     gene            complement(3387..>4372)
                     /gene="carA"
                     /locus_tag="VFC43_01430"
     CDS             complement(3387..>4372)
                     /gene="carA"
                     /locus_tag="VFC43_01430"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HZK29884.1"
                     /translation="AGELVFNTQMTGYMESLTDPSYFGQILMFTFPQIGNYGVDKQNF
                     QNPNICALGCITKEICEKPSALPSFRSFFEENNLLGMSGVDTRALTIKTRVHGTMRAA
                     LVAGSDDGDYAVQLARRTPSISDIVPIPEVSCRAPYHIPGSGKRIAVIDLGIKKNQIT
                     SLANRQGDLYVFPYNARSGQVLACKPDALFISNGPGDPKQAPDAIRCIKDLLGQLPIF
                     GICMGNQVCALALGGDTYKLKFGHRGANQPVRFKDGRIFITTQNHGFAVDADSLPEGC
                     SVSYTNVNDGTLEGFENKDLDLTTVQFHPEAHGGPRDTEAHFFNDLFRRIS"
BASE COUNT         1001 a         1136 c         1102 g         1133 t
ORIGIN      
        1 actaccggtg ccggaggctg ggatgtacag cccgcaataa gaatcccgat aaacaggatc
       61 gcgagaacaa caaataattt cttcatgcat ctttttttgt tatttcggta tataagcata
      121 ttcaattaat ttgtactata caccgttttc tcatggattg tagtatgaga tccccagatt
      181 taaaaccacg ggcaaaaaat agggatccgg ggaaatttac aacgtccccc tcagatagtg
      241 gctgatcggt tccagcgtga ctttttcccg cttgatcgca tcaatagcaa gtgctgcagc
      301 acgcgctgcc tgaagggtgg taatataggg gataccaaag tccactgccg cacgcatgat
      361 ctggtagtgg tcctgccggg actgcttatc ctggggtgtg ttgataatga ggcgtatctc
      421 gccgtgccgc atcatatcga gcacattggg tgaaccttcc tggacctttc ttaccagatg
      481 tgcctctaca cccgcttcgt acaggtaatc aaccgttccc tcggttccgt acagaatgag
      541 ccccaggtcc cggagttgcc gtgctatggg aatagcctct tctttctgct cgatatttac
      601 cgaaataaag atatttcctt tgagcgggag ttcattatcg gcagatatac acgctttgta
      661 aaatgcaagc ccgaaatcat aatcaattcc cattacttca ccggtgcttt tcatctccgg
      721 gccaagcatg gtatcgacac cggcgagctt gttgaaggga aggagcactt cttttacagc
      781 aacatgatgt atcacgcgtt ccttataccc cagatcgcgg agtttccgtc ctatcatgac
      841 ttttgctgca atttttgcca cgggtatccc ggtggctttt gagacaaacg gtacggtgcg
      901 gcttgcccgc ggatttgcct cgaggatata gaccacgttg tccttgactg caagctggag
      961 gttcaccagc ccgactacac cgagcccgag agcgatcttt ttggtataat cccgtatggt
     1021 gtccaggatc tcaggagaca gtgactgtgt cggtatgacg caggcagaat ctcctgaatg
     1081 aatacctgcc tgctcgatat gttccatgat gcctccgata aggacttctt ttccatcgca
     1141 gactgcatcc acgtcgagtt cgacggcgtt ctgaagatag gagtcgatca ggacgggatg
     1201 gttcttgctg acgcggactg cctctttcat gtaggtctca agttcacgct catcgtggac
     1261 gatttccata gcccttcctc caaggacata cgatggcctg acaagtaccg ggtacccgat
     1321 ccgcgctgcc atggccctgg catctccttc agaatacgct gagctgtttg ccgggctggg
     1381 aatattgagg gaagagagaa gcctgctgaa ccggtcgcga tcctctgcaa tatccatggc
     1441 atcaggactc gtgccgagaa ttttagtctt taaccccagc cgtttgatct cctcttcgat
     1501 ggggacggca agatttactg cgttctgccc cccgaactga accatcacac cgtaataata
     1561 atctgtttta aggacattga caacatcctc gagctgcatc ggctcgaaaa agagcctgtc
     1621 tgatgtatca aagtccgtgg atactgtttc aggattgtta ttgatgatat gaacctcgac
     1681 tccttcatcc cgcagagcct ggactgcgtg aacggtgcag taatcgaact cgattccctg
     1741 gccgatccgg ataggtcctg agccgaggat aagaattttc ttgttctcat tggggataat
     1801 ttcacaggcc tgctcatagg tcgaatagaa atacggggtt gttgcgggaa attccgctgc
     1861 acaggtatct accattttgt agctgggaag acctgccatc tcctcgacct gttccaaaga
     1921 aagcccggtg attttcataa gatccgtatt tgggaacccg tatttttttg cagcaaggat
     1981 atcttcaggt tgatgatgct ccaccagatg tttttccata tccacaatat ttttgaattt
     2041 ttctaaaaag aacggggtta tcgatgtaag ctggacgatc tcgtcgattg aaaatccctg
     2101 gcggaaggca tcaaagaggc agtggaaacg ttcatcggtg ggccgggaga ggatcatgcg
     2161 gatctcgctt ggactcgtat gagggatcac atccgtatcg atcgagcgtt ttgccttcat
     2221 gaaggcttcc tcgacagtcc ttccgatcgc catgacctct cccgtgctct tcatggaggt
     2281 ggagagtgtc ctgtcggctc ccttgaactt gtcaaaaggc cagcggggga ccttggcaac
     2341 gatataatca atggctggtt caaacgaagc cggggtgcaa ccggtaaccg tattcatgat
     2401 ctcatcaagc cggagcccga tggcaatctt tgctgccaca cgggctatgg gataccccgt
     2461 tgcttttgag gcaagtgctg atgaacgcga aacacgggga tttacctcaa tgacgcggta
     2521 atccccatcg aggaacgcga actggacatt gcagccaccc tggacgtcaa gtgcacggat
     2581 gatatggata gcggcacttc gcatcatctg gaactcgtcg tcacgaagcg taaggatagg
     2641 agcgaccaca acactttccc cggtatggat acccataggg tccacattct ccattccgca
     2701 gacgataata cacgtatctg aggcatcgcg catcacctcg aattcgatct ccttccagcc
     2761 aaggacgctt tcttcaataa gcacctggtg aatccgtgaa cgggcgagtc cgagttcgat
     2821 gatccggacc agctcctcct ttgtcttgcc aatccctccc cctgctcctc cgagcgtata
     2881 tgccgggcgg acgaccgccg gtaacccgat ggttttgatc gcttcatcga tctggcttaa
     2941 ggaattgata ataatgcttt ttgggacagg ttcacctatc tccgtcatga ggtcacggaa
     3001 tttttcacgg tcttcccctt tataaatcgc ttcaagcggg gtaccgagga tctccatacc
     3061 cttcagggca cccatttccg cgagttcaac agtgaggtta agacccgtct gcccgcccat
     3121 tcccgagagt atgccatccg gcttttcctt ctcgatgatt cttgcaatga catctgctct
     3181 gagaggttcg atgtagattt catcggccat ctccggatct gtcatgatcg ttgcaggatt
     3241 tgagttgacc aggacgacgt taataccctc ttcacggagt gcccggcatg cctgggatcc
     3301 tgaaaaatca aattctgctg cctgccagat ctggatcggg cctgacccga tcatgaggac
     3361 ttttttgata tccggccgtc tgggcatcag gagatcctcc tgaacaggtc attgaagaag
     3421 tgcgcctctg tatctctcgg accaccatgc gcttcagggt ggaactggac agtcgtgagg
     3481 tcgaggtcct tgttctcaaa tccttccagg gtaccgtcat tcacattggt atatgacact
     3541 gagcaacctt cgggcaggga atcagcgtct accgcgaacc catggttctg tgtcgtgatg
     3601 aagatccttc cgtctttaaa ccggaccggc tggttggcac cccggtgccc gaacttgagc
     3661 ttgtaggtat ctcctccgag tgccagcgca cagacctgat tacccatgca tatcccgaat
     3721 atgggtaact ggccaaggag gtcctttatg catcgtatcg catcaggagc ctgtttggga
     3781 tcccccggac cattactgat gaagagtgca tcgggtttgc aggcaaggac ctgaccggat
     3841 ctggcattgt agggaaatac atacagatcc ccctgccggt tggcaagact ggttatctgg
     3901 ttctttttta tgcccaggtc gatgactgca atacgttttc cgcttcccgg gatatgataa
     3961 ggcgccctgc aggagacttc cgggatgggg acgatatctg aaatagaggg agtcctgcgt
     4021 gcgagttgta ccgcataatc tccatcatca ctccctgcaa ccagagctgc acgcatggtc
     4081 ccgtgaacgc gggtcttgat ggtcagcgca cgcgtgtcga caccactcat tccaaggagg
     4141 ttgttctctt caaaaaagga tcggaatgac gggagggcag aaggtttctc gcagatctct
     4201 tttgtaatac atccaagtgc gcatatgttc gggttctgga aattctgttt gtcaacaccg
     4261 taattcccaa tctggggaaa cgtgaacatc aggatctgac cgaaatagct cgggtcagtg
     4321 agggattcca tgtacccggt catctgtgta ttgaacacaa gttccccggc gc
//
LOCUS       DASRLV010000039        23522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_663416,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000039 DASRLV010000000
VERSION     DASRLV010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 23522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23522
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663416"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VFC43_01435"
     CDS             <1..364
                     /locus_tag="VFC43_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HZK29885.1"
                     /translation="AEIDKLFSEIQVDAVAVGCPHCSPDELGEIARLLKGKTVKKPFY
                     IFASQGVIDKNITTVGMIEESGARVYADTCMVVSPVMERYQAIMVNSGKALAYVPDMC
                     GAVARIGSITECVQIATS"
     gene            complement(432..965)
                     /locus_tag="VFC43_01440"
     CDS             complement(432..965)
                     /locus_tag="VFC43_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29886.1"
                     /translation="MVSDIGYDWWELYRSLLILFSSTPKHLEKILHYRDRGDILICNA
                     IYHKVYRQGMGLFHHPQLGYGGELMPDVSQKEVGRRLYHVHREKRVEEAIKKIRHGVG
                     ADWSSFTLEDIDLLGHLLQCTWNTIDQKIWDSLLFANMTKNDVRKILSFGEGVSPGKN
                     PSTEAVVEIKKILLSFK"
     gene            952..1281
                     /locus_tag="VFC43_01445"
     CDS             952..1281
                     /locus_tag="VFC43_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29887.1"
                     /translation="MSETTDDNLGRRLFQVQKEKAVEDAFEKIRRRMGTEWNSVSPAD
                     SEILKEVLGEVWTSIDRHRWGTYSFAKLSKDDINSLVTLGKPMKNHYSLTDEKIRQMD
                     IILGRTV"
     gene            1378..1596
                     /locus_tag="VFC43_01450"
     CDS             1378..1596
                     /locus_tag="VFC43_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HZK29888.1"
                     /translation="MVGWEVPIGAAIAFAAGAIATGWAQSRIGAAGAGAIAEKPETAG
                     SIIIIEAIPETLVILGFVIASMIILMVK"
     gene            1742..2326
                     /locus_tag="VFC43_01455"
     CDS             1742..2326
                     /locus_tag="VFC43_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HZK29889.1"
                     /translation="MAYENLLKSVEESAQERERDLREKARSEVQAISEETKNQTAALK
                     QSLLEEAKRAVTVEKNKRIYLTKGENKEKLIKTKERIFSLAFAEAEQRLSQIRKDPGY
                     PAILKNLTREAVGALGGEKVLVHIDKLDEHLIKGILSDLNSAGEVIPDLHSAGGLVVS
                     TTNESVKISNTIETRLERAKEQKKLQIYAVLYGV"
     gene            2328..3377
                     /gene="ahaC"
                     /locus_tag="VFC43_01460"
     CDS             2328..3377
                     /gene="ahaC"
                     /locus_tag="VFC43_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase A1 subunit C"
                     /protein_id="HZK29890.1"
                     /translation="MDYGYVNARIRAMKSRLLDRHTLEELIFQPDLDSLISSLEKTPY
                     KEDIIEAKVLYQGVLCIEFALRKNFTRTFRKILDFVRKSDAERYIRIFLHRWDVQNIK
                     TILRGKNIHVTNDEILDCLVPAGELDEVTLTELVKQPDVRAVIDLLATWEIVYAKPLT
                     ENFPVYSEKGDLSILECALDKYYFFEALETVSIKSYNNKLIRDLLATEIDVVNIKTIL
                     RMIRDNVDSAEAQEFLIEGGKELPLDILYQLIALHSIEEVIKKLEQTPYRFLADVPEG
                     AIKTKKISVIEKQLEKFLIRKGVSAFSGDPLSVASVIGYFWAKYNEITNIRIISRCKT
                     VDLPDEQLKEELVYV"
     gene            3370..3690
                     /locus_tag="VFC43_01465"
     CDS             3370..3690
                     /locus_tag="VFC43_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HZK29891.1"
                     /translation="MYKFVIVTDSDRASGFRLAGVEVLEAGSPDEAKKVISPLLYKDD
                     IGIVAVNEDFMLALDEKMMNRIEKLARPLIIPIPSKSKELDRKSYIERLLRKAIGYNI
                     VLRR"
     gene            3695..5434
                     /locus_tag="VFC43_01470"
     CDS             3695..5434
                     /locus_tag="VFC43_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HZK29892.1"
                     /translation="MISGTISRISGPVIIARGMKGSKMYDVVKVGDEELRGEIIRLEG
                     NDAVIQVYEDTTGLTIGEKVENTEKPLSVELGPGLLASIYDGVQRPLPVLLEKCGNFI
                     ERGISAPGLDRQKKWEFSAVVKKGDAVADGDVLGTVREFHFEHRILVPHKTSGKVTDI
                     KNGSFTVENVICVLDDTIQLSMMQVWPVRIPRPIQKKLDPILPLITGQRVFDCIFPVT
                     KGGTAMIPGGFGTGKTVSEQTLAKWSDTQIVVYIGCGERGNEMTDVLAEFPELIDPRT
                     GLPLIERTILIANTSNMPVAAREASIYTGITIAEYFRDMGYDVALMADSTSRWGEALR
                     EVSGRLEEMPGEEGYPAYLATRLSAFYERAGRVVCVGGGERTGSVTVVGAVSPPGGDF
                     SEPITQNTLRVVGTFWALDTNLAYRRHFPSVNWIKSYSLYLDSIGDWYVEHVAKDWRD
                     LREKTMYLLQKEVELQEIVQLVGPDALPESEKAILDVTRMVREDFLQQSAYSDTDSFC
                     PMEKQYLMLKVIMTYYTQVNEAMNRGISLKQIQALPLKTNIGRMKDIQDIESIRKMID
                     DISQELSLLEVEK"
     gene            5431..6831
                     /locus_tag="VFC43_01475"
     CDS             5431..6831
                     /locus_tag="VFC43_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HZK29893.1"
                     /translation="MKQASLLTKDYQTINYVSGPLIFVSNVKGASFGEIVRIFLKDGE
                     ERTGQVLDISEDMAVIQVFEGTRGIDTLVTKVRFTGEPARINVSLDMLGRVFNGVGKA
                     RDGGPDIIPESILDIAGTPINPSARDKPADFIQTGMSTIDGLNTLVRGQKLPIFSGSG
                     LPASKLAAQITRQAKVLGEGEQFAVVFVAMGITHKEASFFIRDFESTGALERVVFFMN
                     LADDPTVERLAAPRCGLTVAEFLAFTHNLHVLVILTDMINYCEALREISTAREEVPGR
                     RGYPGYMYTDLSSIYERAGRLKGKTGSITQIPILTMPDDDITHPVPDLTGYITEGQIV
                     LSRDLYRRGADPPVDALPCLSRLMNLGIGPGKTREDHRGVADQLYASYAYGRDLRRLV
                     AIVGEEALTEMDKKYLKFADEIEKRFITQGNEERSIEKTLQIAWDLFAILPEDELKRI
                     KREFIKKYHPAHIGKE"
     gene            6838..7497
                     /locus_tag="VFC43_01480"
     CDS             6838..7497
                     /locus_tag="VFC43_01480"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HZK29894.1"
                     /translation="MEQVKPTRMELMKKKAQIKLAEQGRDLLREKMDALIQEFFKILT
                     TVSNSREELEQVSRSADFALLIAQAVDDPLTLKSASFATRRSITVDISGKNIMGVPVP
                     VIEKKRVSKSMLERGYGIISTSGRIDETAERFEAELDLLIELAETETAMRRLGAEIQM
                     NRRRVNALEQILIPELKNQAKYIKNAIEEREREDLFRLKKVKTIIERKKKAAKKKSLQ
                     L"
     gene            complement(7537..9444)
                     /locus_tag="VFC43_01485"
     CDS             complement(7537..9444)
                     /locus_tag="VFC43_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HZK29895.1"
                     /translation="MKRIQVVGPKKEFNHVVDLLYHEGTIHLEDICQCISQDEIHLYK
                     AEKEKSVEVAEMLSKISSILASLPKIPSDAAAQAAIDNELQDKTRDEIIQRSKKVIKE
                     LEIVTKDLAAKKSDLKFSITTLDRYAKILNIIQPMERELPVLENYEVTILLIQKQFEN
                     ILGLIRQEMDTITGNRFEMSSTSVDDETLATLMIFHKRFSQQVHSFIYSVNVNEVRLP
                     QEYMGKPFYEMFAQIETDKLQKGQEIKFIDEKLSGLSTTWYQELVILKKTFEDISEEL
                     EQVNKFGYSEFTFVVMGWIPKKFLKRMKETITTAYGDRVVIEELDASSEDMEKAPTFY
                     DNPKWIKPFEFVMQLVTPPRYREIDPSPVLAIFFPIFFGLMVGDIGYGLVILAFALIL
                     KNKFRAIEFIKNLADILIISSVPTIIFGILFGEFFGDFGEIMGWIHPVHFLGVTWNRL
                     EAIIPMLIFAIAMGVIHIFLGLIIGIINEVTRKNKKHISEKVGMLLALSGLIVIIALG
                     LEMIPAWTLYPTIIMVIAALVLIIYGAGAMGPLEIMSSVGNILSYARLMAIGLASVIL
                     AFVANKLGGEMEVLAVGVFVAVLLHALNIILAMFSPSIHSVRLHLVEFFSKFYKGGGI
                     AYKPFKKEIKG"
     gene            complement(9483..9803)
                     /locus_tag="VFC43_01490"
     CDS             complement(9483..9803)
                     /locus_tag="VFC43_01490"
                     /inference="COORDINATES: protein motif:HMM:NF028309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit subunit G family protein"
                     /protein_id="HZK29896.1"
                     /translation="MDEERTLLQQIRDKEHEFSKNVETVKQETDAQIGAAKALRENAL
                     VDAELTGKNAAEELFQKEKQKTETEIERMKKASAAETEGAKIKGEKNLQSAVEKIVGF
                     VIME"
     gene            complement(9814..10014)
                     /locus_tag="VFC43_01495"
     CDS             complement(9814..10014)
                     /locus_tag="VFC43_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29897.1"
                     /translation="MVKDLTDTINVLRCTAVVRCRERLEQAAQKARGIIINGQSLDQS
                     GRPLSSKEQYGVTCMMKRKHGL"
     gene            complement(10371..11495)
                     /locus_tag="VFC43_01500"
     CDS             complement(10371..11495)
                     /locus_tag="VFC43_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1646 family protein"
                     /protein_id="HZK29898.1"
                     /translation="MEQLMIVNVMLFCIFILVLVLPFKVKKVEENLEVFLFICGIAAL
                     SVSSFATLHGETFGWTNAIVVEALKAPVMITTVAGIPVGIVQMVLIVGLIIYFAHVQI
                     QQAIQTMVNRVPIYALIFFLICGLGLISSIISAILAAIILVEIVCALPLGRPEKIGVT
                     VISCFSIGLGAALTPLGEPLSTIAISKLSGDPYHAGFGFLLDLLGVYIIPGILAMGIL
                     GVVFLQRQGAPTEGLECKLVREGIRDVVIRAGKVYLFIMALVFLGEGFKPIILEYIIQ
                     IPSEGLFWVNIVSAILDNATLAAAEIGPQLSLIQVKSALLGLLIAGGMLIPGNIPNII
                     AAGKMGITSKEWARYGVPLGVALMLIYFAILFVPAYLGIK"
     gene            11646..12578
                     /locus_tag="VFC43_01505"
     CDS             11646..12578
                     /locus_tag="VFC43_01505"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZK29899.1"
                     /translation="MECVLKYMRRKKSQLYIIVLIVTDLFFLDRFTFSGIFGTDVKKL
                     KEKRNVRGLISALKSKDPIVQYDAAEALGDLRDPLAVDSLTAALKNDELSGVRWKAAE
                     ALSKIGGPAIDALIGVLSHEDDDVRWKAAIALGEIGDSRAVEPLIALLCDEDRFVKSR
                     AAYSLGMIGKAAVEPLIHSLREGDGNLRWGAAIALGKIRDTRAIRPLIWALADKYENV
                     RSESATSLAGFGKPALAPLLQFLKFSEGPMRVEVVTALGELKDSDAIHPLIQLLENAG
                     DEERKAIADALDAILIPSIEPLAAKLRNSNTREE"
     gene            12575..13144
                     /locus_tag="VFC43_01510"
     CDS             12575..13144
                     /locus_tag="VFC43_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZK29900.1"
                     /translation="MKRPVQIIMPEKNDIGNGLPVDEVKIHQLITALKTNSVDTRHHA
                     VRELGAMGKSAVEPLIRELAGAQDNDYRWYVAMALSGLGAAAVVALISAMKSHKDTEF
                     RRYSAAALGNIGIPAIEPLIDAMASDDRQLRGFLSGALCRIGKPAVEPLTRRLEDADE
                     IIRSCATLTLWQMGDTGLPAMVDKFQEDQ"
     gene            13228..17130
                     /locus_tag="VFC43_01515"
     CDS             13228..17130
                     /locus_tag="VFC43_01515"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase II large subunit"
                     /protein_id="HZK29901.1"
                     /translation="MLVLSPAMEAYEKSLMDDLFLAIAIAKKARLVGNDPSTDIEIPI
                     ASDLADRVEALLGIKGVAVRIRELESQMSREEVALRIGDDFVSRRFGETTNEQILDHA
                     IRTAMALLTEGVVAAPTEGIAKIGLGKNDDGSQYLKIFYAGPIRSAGGTAQALSVLVG
                     DYVRRQLGINRYNPRQEEVERYIEEIRQYNTIMNLQYLPSEQEIRLIVENCPVCIDGE
                     ATEQEEVSGHRNLERVETNTVRGGMALVLAEGIAGKAPKLKSKVEKMKMDGWDWLSKL
                     IKNKTDEGTVSIKPLDKYLRDLIGGRPVFSYPMRKGGFRLRYGRSRNTGFAASGLHPA
                     TLYILGEFLAVGTQMKTERPGKACGVVPVDSIEGPTVRMQNGDVLRIDDEAIAKKLGP
                     GIERILDVGEILVSFGEFLENNHPLVPCGYCEEWWQLESPPGTKSPANEDEALALAGA
                     GGYLHPAYTWFWDDITVEQIGALADTVTRQGKISGGFLLLPNEPMAKTILEELLIPHE
                     VRNGNIQIKTYRAFISCLGLDESLQKRDAWKNAPSDNPLNLAMHLSGMKLRSRAGTRI
                     GGRMGRPGKSKPRKMNPPPHVLFPLGDSGGSRRSFQSASNHTAEVDANITDIDFQKEG
                     GIIEIEVGRRRCSECQDITYTNRCDKCGAHTAAITTCPKCGRETFLDRCPGCDVPTTC
                     SQRVFLDVKGEYAKAIGRLGLKSDCVALVKGVKGVISREKTVEAMEKGILRAIQNIFV
                     FKDGTTRFDMIDLPLTHICPDEVRVSVEKLRSLGYMKDHLGYDLQNGSQIVELRPQDI
                     LVSDSCAEYLVNVTQFMDDLLVKFYGLEPFYKVKKPSDLVGHLVIGLAPHTSAGVLAR
                     IIGFTRANVGYAHPFFHAAKRRNCFYGDTEIEVYDGKHWKKTPVRKFVVENFDISRPG
                     IDRLGTYYSDPTQPYFTRTVDTAGTMHLRRITSVSIHRAPPTLLHFVTNLGKEIAVTA
                     DHAMLVWDTTYLRKIRAMELKQGDAIPVLEGVNVIADYIRSVDIVPAPEERVYCLTVA
                     DDHTLAANGIFTGQCDGDEDCIMLLLDGLINFSRSFLPQNRGGSMDAPLVLTSRIDPA
                     EIDKEALNIDVCDHYPLELYTSALAYVEPKTITGLIDRVEKRIGTPAQLEGFQYTHDT
                     SDISAGPIESMYTQLKTMTEKLEAELVLAEKIRAVDADDVAERVLTTHFIRDLMGNLS
                     AFSKQKFRCTKCNTSYRRMPLAGKCTKFKGKGICHGNIIPTVHEGSVKKYLEMSREIC
                     RKYKVSEYTKQRVEVLELAITSTFGEEKQQQLGLADFM"
     gene            17313..17395
                     /locus_tag="VFC43_01520"
     tRNA            17313..17395
                     /locus_tag="VFC43_01520"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17347..17349,aa:Leu,seq:caa)
     gene            17481..17852
                     /locus_tag="VFC43_01525"
     CDS             17481..17852
                     /locus_tag="VFC43_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZK29902.1"
                     /translation="MSRVVHFEFPADNPEQVAEFYKNTLGWKIQKWECPVDYWLIATG
                     EDREPGIDGGIARKKDRPASGVLVTVRVDSVDASLKSIVSNGGSTVVPKRAIPGVGYQ
                     AHFRDPEGNVIGIMENDPSAR"
     gene            18078..18707
                     /locus_tag="VFC43_01530"
     CDS             18078..18707
                     /locus_tag="VFC43_01530"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HZK29903.1"
                     /translation="MDFSLFIQGIIIGLTLAVPVGPIALICIQRTVTDGRFHGIFSGI
                     GVATADSFYAAVTVLGLTIISSVIVTNQFLFRFLAGIVLIFVGMRVFMSLPSCVSSKT
                     VHETYFKDYLSMVGIAIANPLTLVFFLIVLPGFGIVINSTAFLSAAEFIAGVFFGSTA
                     WWILLCGTIGSMRSRFSIKTLRLINRVSGVLISVCGAGMLVLLAMTMGQ"
     gene            18925..19233
                     /gene="eif1A"
                     /locus_tag="VFC43_01535"
     CDS             18925..19233
                     /gene="eif1A"
                     /locus_tag="VFC43_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor eIF-1A"
                     /protein_id="HZK29904.1"
                     /translation="MTDKKPGSPPGDEIIRVKLPQKRNREMFGNAELMMGANHIRVRC
                     FDGVTRMGRIKGKIKKKVWIREGDILIVIPWNFQDEKCDIIYRYTGPQVEWLRRNNYL
                     "
     gene            19517..21166
                     /locus_tag="VFC43_01540"
     CDS             19517..21166
                     /locus_tag="VFC43_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZK29905.1"
                     /translation="MYSLLYVDDEPSLLELAKIFLERTESFSVTTKISAKDGLDALKN
                     GSFDAIISDFAMPGMDGIEFLKIVRAEKPAIPFILFTGKGREEIAIEAIDNGADFYIQ
                     KGGQPKAQFAELAHKIGMAIKRRLAEQSLRDSEQRYKAVVEDQTEFICRFNKDGTYNF
                     VNDAFCRYFQKKPEDLLGKKYKPKIPAKDLPDVRSHFASLTRDNPTAIMVHQIILDDG
                     EVVWHRWSDRAIFNDKGELTEYQSVGRDITRQKKTELELADKKDYLDLIFSSVKAGIV
                     VIDAKTHVIVDINPAAREMMGVDEEEIIGKVCHRFICPAEAGKCPITDLGQCVDNSER
                     TLLTVNGVKKSIIKHVIPTRLYGRNCLLETFLDNTQRKQALEEAQAAYGQLSSYEKEL
                     KQKFEELRESQEKIMESEKVYRAIFENTGTATMIIDEDKTITLINSRAEQLSGYLKEE
                     LEGKRKWTEFVDREDLQRMIAFNDVRWKSPEKVPSQYEFRFSAKDSSVKHILLSIGLI
                     PGTKKAIVSLLDITDYKRMEKKLQESEDRLRILMEKKSEGS"
     gene            21343..23439
                     /locus_tag="VFC43_01545"
     CDS             21343..23439
                     /locus_tag="VFC43_01545"
                     /inference="COORDINATES: protein motif:HMM:NF017078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HZK29906.1"
                     /translation="MDLRKKTLGILTGVIIGIIIIFMVISFTFFLDNYRKMETGYVTD
                     YSNLVLQNVQNEMNNLDMIVKDWGPWDDTYAFVAGEKPDYVAVNLGKPAFQNLRMNFI
                     LITNNQGDILYGEGYDLEKQVITPLRPDIVAELSREGSTLRNLVTTSQPGGFLNLPQG
                     PVILSTYPVLHSDYTGPSRGVVMIGRFLTEAETAKFAYATQAKVSIIPLEQALLSQQD
                     RNALSEADSTQLQVRTLDEDTLEANWVIQDISETGKFVISLKLPRDIYQQGKRDILIF
                     VLLQLGIGLAIGLITIRFLDKQILNRLATINSDIDDITLHKTGRSHIRATGNDEIAHL
                     GLAMNTMIDQIDRDKKELKARELRFREFAEQFPEVMLETDNGLNLTFINTIASEKFGY
                     TQDDLKKNVKILNFIAQEDHVRAEENFSRVLKGLPPSGNDYIMIKKDGSRFPVLLYSA
                     PVIMDEKIVGVRIFAGDISARKQMEKNLLETNRILNLLSRVTRNDISSQLLTLFGYMD
                     LVEETSFEPEKRGYFNKMKQSAENIRQQISFTRDFQDIGVKAPQWQNVKQVILNANGN
                     HDTSRYRVLIDIQDVEVYADKLLERVFYNLVDYANKFGGAISEIRMSALETPEGFRIS
                     LEHDGAGIPEEKKEDIFKEGYFGNTEFGLFLSREILALSGITIRETGDPDKGTRFWIL
                     VPVNRWRKTCLRQTEY"
BASE COUNT         6600 a         5239 c         5744 g         5939 t
ORIGIN      
        1 cgcagaaatt gacaaacttt tttcagagat ccaggttgat gcagttgcag taggctgccc
       61 gcactgttca ccagatgaac ttggagaaat cgcccggctc ctgaaaggaa aaacagtcaa
      121 aaaacccttt tacatttttg cgtcacaggg agtaattgat aaaaatatta ccactgtcgg
      181 gatgattgaa gagagtggtg cccgtgtcta tgccgatacc tgcatggtgg tttcaccggt
      241 tatggaaaga tatcaggcta tcatggtgaa cagtgggaaa gcacttgcct atgtcccgga
      301 tatgtgcggt gcagtcgccc ggattggctc tattacagag tgcgtccaga tagcaacctc
      361 ataatcctgc ttttttacgc cggtttttca gagtatttaa gaaagtcacg atcttttaaa
      421 aaagaaattc tttatttaaa agagagcaga atttttttga tttccaccac agcttctgta
      481 ctcgggttct tccccgggct gactccctct ccgaatgaaa ggatttttct gacatcattt
      541 ttggtcatat tggcgaaaag taacgagtcc cagattttct gatcaatggt attccatgta
      601 cattgcagca gatgacccaa aagatcgatg tcttcgagag taaacgagct ccagtcagct
      661 ccaacaccat gtcttatttt ttttatagcc tcttccactc tcttttcacg gtgtacgtgg
      721 tacaaccgcc ttcccacctc tttttgtgat acatctggca taagttcccc tccatatcct
      781 agttggggat gatgaaacaa tcccattccc tgtctatata ctttgtggta aatggcattg
      841 caaatcagga tatccccccg atcccggtaa tgtaaaatct tctcaagatg cttgggggtg
      901 ctggaaaaca atattaacag ggaacggtat aattcccacc aatcataacc tatgtccgaa
      961 accactgatg ataatctggg aagaaggctt tttcaggtac agaaggaaaa agcagtcgaa
     1021 gatgcatttg aaaagattcg gcgcaggatg ggcaccgaat ggaattcagt ttctcctgcg
     1081 gattctgaaa tactcaaaga ggtcttgggt gaagtctgga catccataga tcgacatcga
     1141 tggggtacct atagtttcgc aaaattatca aaagatgata ttaattccct tgtaactctg
     1201 ggaaaaccaa tgaaaaatca ttattctctt actgatgaaa aaatcaggca aatggatatt
     1261 atactgggca gaacagtata atgctctatt ttatttgaaa atttttacca agaatattta
     1321 caataagttt aaatatctga atctgttagg atactatcat tgaggggggt ttaaaaaatg
     1381 gtaggttggg aagttcctat aggtgcagca attgcctttg ctgcgggtgc gattgcaacg
     1441 gggtgggcac aatcaaggat tggtgcggcc ggagcaggag ccattgcgga aaagccggaa
     1501 acggccggtt caatcatcat catcgaggct attccggaga cactggtcat cctgggattt
     1561 gtgattgcat caatgatcat cctgatggta aaataatctc acgatacaaa atagcgcagt
     1621 gattatatcg cgttcccacg agcaataact tttctccggg ctcccgaacc gggaataaac
     1681 gggagcattc agttgtatta tcaggtcatt gacatgcctt taacaaaaag cgggtgaagc
     1741 gatggcatat gaaaaccttt taaaatcggt tgaagagagc gcgcaggaaa gagagcgtga
     1801 tctcagagag aaagcacgca gtgaagtgca ggcaatttct gaagaaacaa agaaccagac
     1861 agcagctcta aagcagtcgc ttcttgaaga ggcgaaaagg gctgtcactg ttgagaaaaa
     1921 caaacgtatc tatcttacca aaggtgagaa caaggagaaa ctgatcaaaa caaaagaaag
     1981 gattttttcc ctggcatttg ccgaagcaga acaacgcctt tcgcagatac gaaaagatcc
     2041 ggggtatccg gctatcctga aaaacctgac ccgggaagca gttggagcac tgggaggaga
     2101 aaaggtcctt gtccatatcg acaaactgga tgaacacctc ataaaaggga tcctgtccga
     2161 cctgaattca gcaggggagg ttatacctga tctccatagt gcgggtggtc ttgttgtcag
     2221 tacaaccaat gaatccgtaa aaatctcgaa tactattgaa acacggctgg agcgggctaa
     2281 ggaacaaaaa aagctccaaa tctatgcagt cctgtatggt gtttgacatg gattacgggt
     2341 atgtgaatgc acggatccgg gcaatgaaaa gccgtctgct ggaccgacac acactcgaag
     2401 agttgatttt tcaaccggat ctcgattctc ttatttccag tcttgaaaaa acaccgtata
     2461 aagaggatat catcgaggct aaagtcctgt accagggcgt cctctgcatt gaatttgccc
     2521 tgaggaaaaa ttttaccaga accttccgga aaattcttga ttttgtcaga aaaagtgacg
     2581 ccgaacggta tatcaggata ttcctgcacc gctgggatgt ccagaacata aaaacaattc
     2641 ttcgtggaaa aaacatccat gtaacaaatg atgagatact cgactgtctt gtgccagccg
     2701 gggagctcga tgaggtgaca ctgaccgaac ttgtcaagca gccggatgtc agggcagtta
     2761 tcgatttgct tgcaacctgg gaaatcgtct atgcaaaacc cctgactgaa aattttcctg
     2821 tttattctga aaaaggagac ctttccattc ttgaatgtgc acttgataag tattattttt
     2881 ttgaagccct ggaaacggta tcgataaaat cctacaacaa taagctgatc cgcgatcttc
     2941 ttgcaacaga aatcgatgtg gtgaatatca agaccatttt acgcatgatc cgtgacaatg
     3001 tcgatagtgc agaagctcag gaattcctta ttgaaggagg aaaggaacta cctctggata
     3061 tcctgtatca gttaattgcc ctgcattcta ttgaagaggt gatcaaaaaa ctggaacaaa
     3121 caccctaccg gttccttgca gatgttcccg aaggtgcaat aaaaaccaaa aaaatttctg
     3181 tgatagaaaa gcaactcgaa aaatttctga ttagaaaagg agtcagtgca ttttccggtg
     3241 atcccctgag tgttgcctca gttatcgggt atttctgggc caaatacaac gaaataacta
     3301 atataagaat aatttcacgg tgtaaaaccg tggatctccc ggatgaacag ctcaaagagg
     3361 aactggtgta tgtataaatt tgtgattgta acggatagcg atcgtgcttc aggtttccgt
     3421 cttgcaggag ttgaagtact tgaagcaggt agtcctgatg aagcaaaaaa agtaatttcc
     3481 cccctgttat acaaagacga tatcggaatc gtcgcagtaa atgaggattt tatgcttgcg
     3541 cttgatgaaa aaatgatgaa ccgtatcgaa aaactggccc gcccacttat catcccgatt
     3601 ccatcaaaat ccaaggaact tgacagaaaa tcctatattg aacgactttt gcgtaaagca
     3661 atcgggtata acatcgtttt gcggaggtga aggtatgatt tcaggaacaa tatccagaat
     3721 ttccggccct gtcattatcg cccggggcat gaaagggtca aaaatgtatg acgtggtgaa
     3781 agtcggggat gaggagctga gaggagagat tattcgcctg gaaggaaacg atgcggttat
     3841 ccaggtttac gaagatacga ctggtctcac cattggggaa aaagtagaga atacagaaaa
     3901 acctctctcc gttgaacttg gccccgggct tcttgcctct atttatgatg gtgtgcaacg
     3961 gccccttcct gttcttcttg aaaaatgcgg gaattttatt gaacggggca tttcagcacc
     4021 aggactcgac cgtcagaaaa aatgggagtt ttctgcagta gtaaaaaaag gcgacgccgt
     4081 tgcagatggt gatgtgctgg ggactgtccg cgaattccat ttcgagcaca ggatccttgt
     4141 tccccacaaa acctccggca aagtaactga catcaaaaat ggttcgttta ccgttgaaaa
     4201 tgtgatatgc gttcttgatg atacaatcca gttgtccatg atgcaggtct ggccggtcag
     4261 gattccccga ccaatacaaa aaaagcttga tcctattctg ccgctgatta ccggccagag
     4321 agtatttgat tgtattttcc ctgtcacaaa aggtggtact gcgatgattc cgggcggttt
     4381 tggaacagga aagactgttt cggaacagac acttgccaaa tggtccgata cgcagattgt
     4441 cgtatacatc ggatgtgggg aacgcgggaa cgagatgacc gatgtccttg ctgaatttcc
     4501 tgaacttatc gatccgcgta caggtctccc gcttatagaa cggacgatcc ttatcgcaaa
     4561 cacctctaat atgccggttg ctgcacggga ggcttcgata tataccggaa ttaccatcgc
     4621 agagtatttc agggatatgg ggtatgatgt tgcgctgatg gcagattcaa cctcacggtg
     4681 gggtgaggca ttacgggaag tatccggacg actggaagag atgcccggtg aagaggggta
     4741 tcctgcctac ctcgctacac ggctttctgc tttttatgaa cgcgccggca gggttgtgtg
     4801 tgttggcggg ggagaacgaa cgggatctgt gacagtagta ggtgccgtct ctcccccggg
     4861 gggtgacttc tcggagccta tcacccagaa cacattgcgg gttgtcggaa cattctgggc
     4921 gctggatacc aacctcgcat accgcagaca ttttccgtca gtaaactgga taaaaagtta
     4981 ctccctgtac cttgacagta tcggggactg gtatgtcgag catgttgcaa aagactggcg
     5041 ggatctcagg gaaaagacca tgtacctttt gcaaaaggaa gtcgaactgc aggaaatcgt
     5101 ccagctggtc ggcccggatg cgctacctga gagcgaaaag gcaattcttg acgtgacccg
     5161 gatggtcagg gaggacttcc tccagcagag cgcctacagc gatacagatt ctttctgccc
     5221 catggaaaag cagtatctca tgctcaaggt catcatgacc tactataccc aagttaatga
     5281 ggcgatgaac cggggaatct cattaaaaca aatccaggca cttcccctta aaacaaacat
     5341 tgggcgcatg aaggatatcc aggatattga aagcatccgg aaaatgattg atgatatcag
     5401 ccaggagctc tcattgctcg aggtggaaaa atgaagcagg catcacttct tacaaaagat
     5461 taccagacta tcaattatgt atcaggcccc ctgatctttg tcagcaacgt caaaggtgcc
     5521 tcgttcggcg aaatcgtcag gatttttctc aaggatggag aggaaaggac cggacaggtt
     5581 cttgacatct ccgaagacat ggctgtcatc caggtatttg agggcacgag aggaatcgac
     5641 acgctggtga caaaagtcag gttcacgggt gaacccgccc ggatcaatgt atctcttgat
     5701 atgctgggcc gcgtattcaa tggtgtaggg aaggctcgtg acggtggccc ggatattata
     5761 ccggaatcta ttcttgatat tgccggaaca cccataaatc catcagcacg ggacaaacct
     5821 gcagatttta tccagactgg tatgtccact atagatggtc tgaatactct tgtacgcggt
     5881 cagaagctgc ccatcttctc gggatccggt ctgcccgcga gcaaacttgc agcacagatt
     5941 acccggcagg ctaaagtcct tggggaaggt gaacagtttg ccgtagtatt tgtagcaatg
     6001 ggaatcaccc acaaggaagc ttcattcttt atccgggatt ttgagagtac cggagcgctg
     6061 gaacgggtgg tcttttttat gaaccttgcg gatgatccga cggtcgaacg tctggcagct
     6121 ccccgttgcg ggctcacggt tgctgaattt ttagcattta cccacaacct tcacgtgctt
     6181 gttatcctga cggatatgat caattattgt gaagctctcc gtgagatctc aacagcccgc
     6241 gaggaggttc ctggcaggag aggttatccc ggatatatgt ataccgatct ctcctcaatt
     6301 tacgaacggg caggcaggct gaaaggaaaa accggctcca tcacacagat acctatcctg
     6361 acgatgcctg atgatgatat aacccatccg gtgcccgatc tgaccggata tatcaccgaa
     6421 ggacagattg tgctgtcccg cgatctttat cggagaggtg cagatcctcc ggttgacgca
     6481 cttccctgcc tttcgaggct tatgaacctg gggataggtc ccggtaaaac ccgtgaagac
     6541 caccgtggtg ttgccgacca gctgtacgcg tcctatgcgt atggcagaga tcttcgcagg
     6601 ctggtggcaa ttgtcggaga ggaagctctt acagaaatgg ataaaaaata cctcaagttt
     6661 gcagatgaga tagaaaaacg gtttatcacg cagggcaatg aagaacgctc aatcgaaaag
     6721 acccttcaga ttgcatggga cctctttgcc atcctcccgg aagacgaact caagcggatc
     6781 aaacgtgagt ttatcaaaaa atatcatcct gcgcatatcg ggaaggaatg atgaaatatg
     6841 gaacaggtaa aaccgacgag aatggagctg atgaaaaaaa aggctcagat aaaacttgca
     6901 gagcagggcc gggatctttt gcgggaaaaa atggatgcac tcatccagga attttttaag
     6961 atcctgacta cggtttcaaa ttcccgcgaa gagctggagc aggtttcgcg ttccgcggat
     7021 tttgccctcc ttatagcgca ggcagtagat gatcctctta ccctgaaatc tgcatctttt
     7081 gccacccgga gatctattac cgtggatatc agcggaaaaa acatcatggg tgtccccgtc
     7141 ccggttattg agaaaaaacg tgtctcaaaa agcatgctgg agaggggcta tggtatcata
     7201 tcaacgagcg ggagaatcga tgaaacagct gagcgatttg aagctgaact tgatcttctc
     7261 atcgaacttg ctgaaaccga aaccgccatg agaagactcg gtgctgaaat ccagatgaac
     7321 cggcgccggg taaacgctct tgaacagatc ctgatccccg agttgaaaaa tcaggccaag
     7381 tacattaaaa atgcaattga agaacgggag agagaggatt tattccggtt gaagaaggta
     7441 aaaaccataa tcgagagaaa gaaaaaagcg gcaaagaaaa aatccctgca actataatga
     7501 aaagatgtaa ttatgcgggg gatggggaag attcagttac cccttaatct cttttttgaa
     7561 aggtttgtac gcaattcctc cgcctttata gaactttgag aaaaattcca cgaggtgcag
     7621 tcgcactgaa tggatagaag gagaaaacat ggcaagaata atattgagtg cgtggaggag
     7681 tacagcaacg aaaactccga ctgcaagtac ttccatttct cctccaagct tgtttgccac
     7741 aaaagccagg attaccgagg caagaccaat tgccatcaac ctggcatagg atagaatatt
     7801 acccaccgaa ctcatgattt ccagagggcc catggcacca gcaccataga taatcaacac
     7861 aagtgcagcg ataaccatga tgattgtggg gtagagggtc catgccggaa tcatctcaag
     7921 acccagtgca atgataacga tgagtccgga tagtgcgagc agcatcccga ctttctcaga
     7981 tatatgtttt ttgttttttc tggttacttc attgataatc ccgatgataa gacctaaaaa
     8041 gatatggatg acacccatgg cgattgcaaa gatgagcatg ggtataattg cttcaagccg
     8101 gttccaggtg accccaagaa aatgaacagg gtgaatccag cccatgatct ccccgaaatc
     8161 cccgaaaaat tctccaaaca gaattccgaa aattattgtc ggtactgaag aaataatcag
     8221 aatgtctgca aggtttttta taaattctat ggccctgaat ttatttttca ggatcagcgc
     8281 gaaagcaaga atcaccagac cgtacccgat atcgcccacc atcagcccaa aaaatatggg
     8341 aaaaaagatc gctaggaccg gggagggatc gatctccctg tatctgggcg gtgtcaccag
     8401 ttgcatgacg aactcaaatg gcttgatcca cttggggtta tcataaaagg taggcgcttt
     8461 ttccatatct tcagagctcg catcgagttc ttcgattacg actctgtccc cgtatgcagt
     8521 tgttattgtc tctttcattc gtttcagaaa tttttttggg atccatccca taacaacaaa
     8581 agtgaattcg gaatatccga atttattaac ctgctcaagc tcttcgctga tatcttcgaa
     8641 ggtttttttg agtattacca gttcctgata ccatgttgtt gaaagcccgg aaagcttttc
     8701 atcgataaat tttatctcct gtcctttctg gagtttatcg gtttcaatct gagcaaacat
     8761 ctcgtaaaac ggcttcccca tatattcctg tgggagacgg acttcgttaa cattaaccga
     8821 ataaataaat gaatggacct gctgggaaaa gcgtttatga aagatcatta aggtagcgag
     8881 tgtttcgtca tctacagaag ttgaagacat ttcaaaccgg tttccggtga tcgtatccat
     8941 ctcttgcctg atgaggccca gaatattctc aaactgtttc tgtataagaa gaattgttac
     9001 ttcataattc tcgagcacgg gaagctcacg ttccataggc tggataatat tgagaatttt
     9061 tgcatacctg tcaagagtag taatggaaaa tttcaggtca ctttttttgg cagcaagatc
     9121 ttttgttacg atttcgagtt ctttaattac ttttttagac ctttgaatta tttcatcccg
     9181 ggttttatcc tgtaattcat tgtcaatggc tgcctgtgct gctgcatcag aaggaatttt
     9241 gggaagggaa gcaaggatac tggaaatttt gcttaacatt tcggcaactt ccacggactt
     9301 ttccttctct gctttgtaaa gatgaatctc gtcttgggag atgcattggc atatatcttc
     9361 aagatgaatg gttccttcgt gatatagcag gtcaactacg tgattaaatt ctttttttgg
     9421 cccgactacc tggattcttt tcattttctg gagcataaaa actcaaggtt tgagaaaccg
     9481 tactattcca tgataacaaa accgacgatt ttttccactg ctgattggag atttttttca
     9541 ccttttatct ttgcaccttc tgtttcagca gctgatgctt ttttcatccg ctcaatctca
     9601 gtttctgtct tttgcttttc cttttgaaag agttcttcag cagcgttttt tcctgttagc
     9661 tcggcatcaa ccagagcgtt ctccctcaac gcttttgccg caccgatctg tgcatctgtc
     9721 tcctgtttta cagtttcgac atttttggaa aattcatgtt ctttatcccg tatctgctgc
     9781 agcagggttc tttcctcgtc catatatcct ccgtcataat ccatgcttgc gtttcatcat
     9841 gcaggtaaca ccatactgtt ctttagaact gaggggcctc ccgctctgat cgagtgactg
     9901 accgttaata attatgcctc tggctttttg agcagcctgc tcgagccgct cccggcatcg
     9961 tacaacggca gtacatcgca atacattgat agtatctgta agatccttta ccataaccag
    10021 ccaaatgttt ttataatcaa ttggactata ccgtataagt taaataaata ctttcctgtt
    10081 cttaggaaat ggactgcaac ggcaaaagag aagggcggtc tgaaaagagg tttttttgcc
    10141 ccatgagggt cattgaatcc aacaaataaa agggtaatgg catagaaaaa gagtaccgct
    10201 gaccatcgtt ggtctcatca gtattggagc agcactcccc catcgtggtg aaaaaagggg
    10261 taagccaatg ttctatgcca aatgcgtgcg gtccggtcgc ccggatcgga tgatgaaaat
    10321 tgtattgcag tggatccggg ttaatccgac cactgtacaa aagagggagc ttattttatc
    10381 cccaggtacg cggggacaaa caggattgca aagtagatca gcataagggc aaccccgagc
    10441 ggcacaccgt accgcgccca ttccttactg gtgattccca ttttacccgc tgcgataata
    10501 ttgggtatgt taccgggaat cagcattccc cctgcgatca aaaggcccaa taaagcgctt
    10561 ttaacctgta tcaggctgag ttgaggccca atttcagctg ctgcgagtgt tgcattatcc
    10621 aggattgccg acacaatatt cacccagaaa agtccttcgg aaggtatctg gataatatac
    10681 tcaaggatga ttggtttgaa cccttctccg agaaagacca gtgccatgat aaacagatat
    10741 acttttcctg cccggatcac tacgtcgcgt atgccttcac ggacaagttt acactcaagc
    10801 ccctcggtgg gagcaccctg gcgttgtaaa aaaacaacac caagaatccc cattgcaagg
    10861 attcccggga tgatatagac ccccagcaga tccaaaagga acccaaaccc cgcatggtaa
    10921 ggatctccgg atagtttcga gatagcaatg gtagagagcg gttcacccag gggggtgagt
    10981 gccgctccga gaccaatgga aaaacaggag atgaccgtta caccaatctt ctctggtcgt
    11041 cccagtggca gggcgcatac gatctcaaca agaataattg cggcaaggat agcagatatg
    11101 atgctggata tcagtccaag accacagatc aggaagaaga taagtgcata aatgggcacc
    11161 cggtttacca tagtctggat agcctgctgg atctgaacat gggcaaaata gattatcagg
    11221 ccaacgatca ggaccatctg gacaatacct actggaatac cggcaacagt cgtaatcatg
    11281 accggcgctt tgagcgcttc aacaactatg gcgtttgtcc atccgaatgt ctccccgtgc
    11341 agggttgcaa agctggaaac gctcagtgca gcaattccgc agatgaagag aaatacttca
    11401 aggttctctt caactttttt gactttgaac ggtagtacca gcaccaggat gaatatacaa
    11461 aaaagcatga cattgacgat catcagttgt tccataatta ccgatcccgg ggattttgct
    11521 cctgatgact attatttctc tgcggattta aaaaatgatg atcccggata acgtggttta
    11581 aatcctgatg tttttgcagg caggagacaa ttcatttatc tgttttattg ttatacaatc
    11641 atcagatgga gtgcgtcctg aaatacatga ggcgaaaaaa aagccagctg tacataatag
    11701 tgctcatcgt gacggacctg ttttttttgg accgtttcac gttttcaggc atctttggaa
    11761 cagatgtcaa aaagctgaaa gaaaagcgaa atgtccgagg attgatctca gcactgaaaa
    11821 gcaaagatcc aattgtgcaa tatgatgcag cagaggccct tggggatctg cgggacccac
    11881 tagcggtgga ctccctgacc gccgcattga aaaacgacga actgagcgga gtccggtgga
    11941 aagcggcaga ggccctttcc aaaatcgggg ggcctgccat tgacgcactt ataggagtac
    12001 tctcccatga agatgatgat gtgcgctgga aggcagctat tgcacttggt gagatcggtg
    12061 attcaagagc tgttgaacca cttatcgccc tgctttgtga cgaggaccgt tttgtcaaaa
    12121 gccgcgccgc gtattctctt ggcatgattg gaaaagcggc ggtggaacct ctcattcatt
    12181 ccctcaggga aggtgatgga aatctccgct ggggggctgc tatcgccttg gggaagatcc
    12241 gcgatacacg ggctatcaga ccactgatct gggcacttgc ggacaaatat gaaaacgttc
    12301 gttccgaatc tgccacctca cttgcaggat ttggaaaacc tgcattggcc cctctcctgc
    12361 agtttttaaa attttccgag gggccgatgc gggttgaagt ggtcacggcc ctgggagagc
    12421 tgaaagattc agatgcaatc catccgctga tccagttgct tgaaaacgct ggtgatgaag
    12481 aaagaaaagc gatagccgat gcacttgacg caatcctgat tccctccatc gagcctcttg
    12541 cggcaaaact tcggaatagt aataccagag aagaatgaaa agaccagttc agatcatcat
    12601 gccggagaag aatgatatcg ggaacggact tcccgtggat gaggtgaaaa ttcatcagct
    12661 cataaccgct ctgaaaacca attctgtcga tacccggcac catgctgtgc gggagcttgg
    12721 tgcaatggga aaatctgctg ttgaaccgtt gatccgagaa cttgctggag cacaggataa
    12781 tgattaccgc tggtatgtgg caatggcact ttccggcctc ggagccgccg ctgtcgttgc
    12841 actgatctcg gcaatgaaat ctcataagga tactgaattc cgtcgctatt ctgcagcggc
    12901 actgggaaat ataggtattc cggcaattga gccgctcata gatgcgatgg caagcgatga
    12961 caggcagttg aggggatttt tgtccggtgc cctctgcagg attggaaaac ccgcggttga
    13021 accccttacc cgccggctgg aagacgcgga tgaaatcata cggtcatgtg caaccctgac
    13081 tctctggcag atgggcgaca caggacttcc ggcgatggtt gataaattcc aggaagacca
    13141 ataaaacgac cggcatctta agtttcaata gtcttatttt cgtcataaca cgaacagata
    13201 aattcacgcc cgcaaaatcg tacccatatg ctcgtgcttt ctccagcaat ggaggcctat
    13261 gaaaaatctc tgatggatga ccttttttta gcaatagcga tagccaagaa agcccgtctt
    13321 gtcggtaatg acccgtcaac cgatattgag atccccattg ccagtgacct tgcagaccgg
    13381 gttgaggcgc tcctgggaat caagggagtg gcagtacgga tccgtgagct cgaatcgcag
    13441 atgtcccgtg aagaggttgc attgcggata ggcgatgatt ttgtttcccg cagatttggt
    13501 gagaccacaa acgaacagat cctcgatcat gccattcgta cggcaatggc cctgctaact
    13561 gaaggtgttg ttgcagcacc caccgaaggg atagccaaaa tcgggctcgg gaaaaacgac
    13621 gatgggagcc agtatctgaa aatcttctac gcgggaccga tccggagtgc gggggggact
    13681 gcccaggcac tctcggtgct ggttggcgat tacgtccgcc gccagctcgg cataaaccgt
    13741 tacaatcccc gccaggaaga ggttgagcgg tatatcgagg agattcggca gtacaacacc
    13801 atcatgaacc tccagtacct gcccagcgaa caggagatca ggctgatcgt agaaaactgc
    13861 ccggtatgta ttgacgggga agcaacggaa caggaagaag tatcaggtca ccggaatctc
    13921 gagcgtgttg agacgaacac ggtgcggggc gggatggcac tggtgcttgc agaaggcatt
    13981 gcagggaaag caccaaaact caagagcaaa gtagaaaaaa tgaagatgga cggctgggac
    14041 tggctttcaa aactcatcaa aaataaaacc gatgaaggga cagtcagtat caagcccctg
    14101 gacaagtacc ttcgtgacct gatcggcggc agaccggttt tttcttaccc gatgagaaaa
    14161 ggcggcttca ggctccggta cgggaggtca cgcaataccg ggtttgcagc ttcggggctc
    14221 caccctgcga cgctctacat ccttggcgaa tttcttgcgg ttggtaccca gatgaagaca
    14281 gaacggccgg gaaaggcgtg cggtgtggtt ccggttgatt ccattgaagg gccgacggtg
    14341 aggatgcaga atggggatgt gctcaggatc gatgatgaag ctatagcgaa aaagctcgga
    14401 cctggtattg aacggattct tgatgtggga gagatcctcg tctcgtttgg tgaatttctg
    14461 gagaacaacc acccccttgt gccatgtggc tattgcgagg agtggtggca gcttgaatcc
    14521 cccccgggta caaaatctcc ggcaaatgaa gatgaagccc ttgcccttgc aggtgcagga
    14581 gggtatctcc atcctgcgta tacctggttc tgggatgata ttaccgttga gcagataggt
    14641 gcccttgcgg atactgtcac gcggcaggga aaaatttccg gtggcttcct gctgctgccc
    14701 aatgaaccaa tggcaaaaac cattctcgaa gaacttctta tcccgcacga ggtgcgtaac
    14761 gggaatattc agatcaaaac gtatcgtgct tttatttcct gccttggact tgatgagagt
    14821 ctgcaaaaac gcgatgcatg gaagaatgcg ccgtctgaca atccgctcaa tcttgcaatg
    14881 cacctctccg ggatgaaact caggtcacgg gcagggaccc ggattggcgg gcggatgggc
    14941 cggcccggca aatcgaaacc cagaaaaatg aatcccccac cccatgtgtt attcccgctc
    15001 ggcgactcag gaggctcccg gcgatcattc cagtccgcat ccaaccatac cgcggaagtg
    15061 gatgccaata tcaccgatat cgatttccag aaggaaggcg ggataattga gatcgaggtc
    15121 ggaagacgaa ggtgttctga gtgccaggat attacgtata ccaaccggtg tgacaaatgc
    15181 ggggctcata cggctgcaat tactacctgt cccaaatgcg ggagagaaac ctttttagac
    15241 cgctgccccg gctgtgatgt gccgactacc tgcagccagc gcgtctttct ggacgtaaaa
    15301 ggagaatatg caaaagcaat aggcaggctg ggtcttaaat ccgattgtgt ggcactggta
    15361 aagggggtaa aaggggtaat atcccgggaa aaaacagtcg aagcaatgga gaaagggatc
    15421 ctccgggcca tccagaatat ctttgttttc aaggatggga ccacgcggtt tgacatgatc
    15481 gacctgccac tcacacacat ctgtcctgac gaagtgcgtg tttctgtaga gaaactccgt
    15541 tcgctggggt acatgaaaga tcatcttgga tacgatctcc agaacggatc gcagattgtt
    15601 gaattacgcc cgcaggatat tttagtctct gattcgtgtg ccgaatacct ggtaaacgta
    15661 acgcagttca tggacgacct tctggtgaaa ttctatggac ttgaaccctt ctataaggtt
    15721 aaaaaaccaa gcgatctggt cggacacctt gtcattggtc ttgctcccca tacaagcgcc
    15781 ggtgtacttg caaggatcat cgggttcacc agggccaatg tcgggtacgc ccatcccttc
    15841 ttccatgccg ccaagcggcg aaactgtttc tatggggata ccgagataga agtgtatgat
    15901 ggaaagcact ggaaaaaaac acctgtccgg aaatttgttg ttgaaaattt tgatataagc
    15961 cgcccgggta ttgaccggct ggggacctat tattccgatc cgacacagcc atattttacc
    16021 cgcaccgttg ataccgcggg gaccatgcac ctccgccgga taacgtcggt atccatacat
    16081 cgggcccccc cgacccttct ccattttgtg acaaatcttg gaaaagaaat tgccgtaaca
    16141 gctgaccatg caatgctggt atgggatacg acttacctcc ggaagatccg ggcaatggaa
    16201 ctgaaacagg gtgacgccat cccggtactc gaaggtgtaa acgtgatagc tgactatatc
    16261 cgctcggttg atattgttcc tgcacccgaa gagagggtct attgcctgac ggttgctgat
    16321 gaccatacgc ttgctgcaaa cgggattttc actggccagt gcgatggtga tgaggactgc
    16381 atcatgctac ttttggacgg ccttatcaat ttttcacggt ctttccttcc tcagaaccgg
    16441 ggaggttcta tggatgcccc cctcgtactg accagcagga tcgatccagc agaaattgat
    16501 aaggaagccc tgaatatcga tgtgtgcgac cattacccgc tggaactgta cacaagcgct
    16561 ctagcctatg ttgaacccaa aacgataacc ggtcttatcg accgggttga aaagcggatc
    16621 ggcacaccgg cacagctcga gggattccag tatacacacg acacttcaga tatatcggcc
    16681 gggcccattg aatcgatgta tacccagctc aagacgatga ccgaaaagct cgaggcggag
    16741 cttgtccttg ctgaaaagat acgtgcggtc gatgcagacg atgtcgccga acgggtgctt
    16801 acgacacatt tcatccgcga tctgatggga aatctttcgg cgttttccaa acagaagttc
    16861 cggtgcacca agtgtaacac gagttaccgg cggatgccac tggcagggaa atgcacaaag
    16921 tttaagggaa agggaatctg ccacggcaat atcatcccga ccgtgcatga aggttccgtg
    16981 aaaaaatatc ttgagatgtc gcgggagatc tgcaggaaat ataaggtatc tgaatacacc
    17041 aagcagaggg tcgaagtgct tgaacttgca attacttcga cttttggtga ggagaaacaa
    17101 caacagctcg gtcttgctga ttttatgtag gagtgaatcg tggacaggtt ttttagtttt
    17161 ttttcatgga tgggaaaaac aagagtaatt tcaaagaggg atactcacgt ggtcaccggt
    17221 gatcgactca ggatttactg gaccggtttg gcataggagc acgtttttta aaactcaaag
    17281 ccttttacct tccaatacca acattattgt aagcgagaat atctaagtgg tcaacagagg
    17341 cggactcaag atccgttcgc gcaggcgttc gcaggttcga ctcctgcttc tcgcatcctg
    17401 tttttatgta aggtttcatt gtaacatcga tagcaatcct tgataatcag gaaagatgaa
    17461 atgacctctt gtgatatctc atgtcccgcg ttgttcattt tgaatttcct gcagataatc
    17521 cggagcaggt ggcagagttt tataaaaaca ccctgggctg gaagattcag aaatgggaat
    17581 gtccggtcga ttactggctg atcgcaaccg gggaggaccg tgaaccggga attgacggtg
    17641 gtatagcaag aaaaaaagac cgcccagctt ctggcgtgct cgtcaccgtc cgggttgact
    17701 cagttgatgc atcccttaaa agtattgtca gcaacggagg gagtaccgtt gtgccgaaga
    17761 gggcgatacc cggtgtgggg taccaggccc atttcaggga tccggaaggg aatgtgatcg
    17821 ggatcatgga aaatgatccc tcagcacggt gagacatggg gtgtgtcacc gaaaccaaac
    17881 cattacacac gtcctgaaat accgttacag cctctcaacc tgccactttt ttgttggtat
    17941 gcaagattga tgacgggtcg aaatcaatag aatagtactt caatcctgga gtggtttcgt
    18001 caaatgaaaa aaggctgccg gatatacctc gaaatatcct tccgggacaa gcgtttcgaa
    18061 aaattatatg gtgctgcatg gacttcagtc tctttattca gggaataatc atcggtctga
    18121 ccctcgcagt gccggtcggt ccgatcgccc tcatctgtat ccagcggact gtaacagacg
    18181 gcaggttcca tgggatcttt tctggcattg gtgtggcaac tgccgattca ttctatgcag
    18241 cggtcaccgt tcttggactc acgataatct cgagcgtgat cgtcaccaat cagtttcttt
    18301 tccggttcct tgccgggatt gtcctgattt ttgtaggtat gcgggttttc atgtcgctgc
    18361 cctcgtgcgt cagctcaaag actgtgcacg agacgtattt caaagattac ctgtccatgg
    18421 ttgggattgc aattgcaaac cccctgaccc tcgtcttctt tctgatagtt cttccgggat
    18481 ttgggattgt gatcaatagc actgcatttc tgtctgcggc ggaatttatt gccggtgttt
    18541 tttttgggtc tacagcctgg tggattctgc tctgtggtac tatcggatcc atgcggtccc
    18601 gtttcagtat aaaaaccctc agactgatca atcgcgtatc cggggtcctt atttccgttt
    18661 gtggtgcggg aatgctcgtc ctgcttgcga tgacgatggg acagtaaacg tgacccgacc
    18721 ggttttcaca gggtttcttt tatcagaaag gtaattcctt gattgtgcgg tttgaccccc
    18781 tttaaaggat agttctgaag cctctatgag ctgtttttcc ggaattccgg tccccttgga
    18841 aggaataatt atattttctt ctgcgctaaa aattgagcac taaataaaaa gccacaaaaa
    18901 agcacaaaaa gaggagaggt tgtactgaca gataagaaac cgggatcacc tccgggtgat
    18961 gagatcatac gggtaaaact accccagaaa cgaaaccggg aaatgttcgg caacgccgaa
    19021 ctgatgatgg gtgccaacca tatccgggtc cgttgtttcg atggtgtcac ccggatggga
    19081 agaatcaagg gaaagatcaa aaagaaagtc tggatccgtg aaggggatat cctcatagtc
    19141 atcccctgga acttccagga tgaaaaatgt gacatcatat accggtacac cggaccacag
    19201 gtcgaatggc tccggcgcaa taattatctt tgaaaccggt ggggtaatct tttttatttc
    19261 gaccaagagg cattctgttt aattcctctc aattttcggg cgttctgatt tgaagtcgcg
    19321 actgctctac ctatatcaca tctagacaag ttattgtttt tgctctccag attttcatta
    19381 ttggtatccg gcagcacctt tttaaggtaa atatcgctta tatgttagag aaggttatta
    19441 tactgaaata aaaaaaaata aatacattga atatctttga tgaacaggga aagtttctga
    19501 ttaaagggta aattatatgt attcgttgtt gtacgttgat gatgagccat cgctgcttga
    19561 gctggccaag atttttcttg aacgtacaga gtccttttcc gttactacaa aaatatcggc
    19621 aaaagatgga cttgatgcac tgaagaacgg ttcttttgat gcaatcattt ccgatttcgc
    19681 gatgccggga atggatggga ttgaattctt aaaaatagta cgcgcagaga aacctgcaat
    19741 ccctttcatt cttttcacgg gtaaaggaag agaggaaatc gccatagaag cgatcgataa
    19801 cggtgcagat ttttacattc agaagggagg acagcccaaa gcccagtttg ctgagctcgc
    19861 ccataagatc gggatggcaa ttaaacgcag gcttgctgaa caatcactca gggattcaga
    19921 gcagcgttac aaggcagtcg tcgaggacca gaccgaattc atctgccgtt tcaataaaga
    19981 tgggacatat aacttcgtaa atgatgcatt ttgccggtat ttccagaaaa aacctgaaga
    20041 tctccttggt aaaaaatata agcccaaaat cccggcaaag gatcttcctg atgtcagaag
    20101 ccatttcgcc tcgctgaccc gcgataatcc caccgcaatc atggtccacc agattatatt
    20161 ggatgatggg gaggtagtat ggcaccggtg gagtgatcgg gcgattttta acgacaaagg
    20221 agagcttacc gaataccagt ccgtgggacg ggatataacc cgccagaaaa aaaccgaact
    20281 ggaactcgca gataaaaagg actatctcga cctgatcttt tcatccgtta aagccggcat
    20341 tgttgtcatt gatgcaaaaa ctcatgtgat tgtagatata aatcccgccg caagggagat
    20401 gatgggtgtt gacgaagagg agatcatagg gaaggtctgc cacaggttca tctgtcccgc
    20461 cgaagcaggg aaatgcccaa tcacggattt ggggcagtgt gttgataact ccgaacgcac
    20521 cctgcttacc gttaatgggg tgaagaaatc cattatcaaa catgttatcc ccaccagatt
    20581 gtatgggcgt aactgtctgc ttgaaacatt tttagataat acacaacgaa aacaggcatt
    20641 agaagaagct caggccgctt acggacagct cagttcctat gaaaaagagc tgaaacagaa
    20701 atttgaagaa ctccgggaaa gtcaggaaaa aatcatggaa tcagaaaaag tataccgggc
    20761 aatttttgaa aataccggta ctgctaccat gattattgat gaggataaga ccataactct
    20821 tatcaatagc agagctgagc agctttcagg ttatttgaaa gaagaacttg aaggcaaaag
    20881 aaaatggaca gaattcgtgg acagggaaga ccttcaacga atgattgctt tcaatgatgt
    20941 acgatggaaa tctcccgaaa aggttccttc acagtatgaa ttcagatttt ctgcaaaaga
    21001 ttcgagtgta aaacatattc tgttgtccat cggcctcatt cctggtacaa aaaaagcgat
    21061 agtatccctt ctcgatatca cagactacaa aagaatggag aaaaagttac aagagagcga
    21121 ggatcgattg aggatcctga tggaaaagaa atcagaagga tcatgataac ggttcccgat
    21181 attttcgatg ttattttcca tcataacatt ccgcatcatt ttcttcatgg cataaccagt
    21241 cggaagtatt gtgaggttcc gggttgcttt gtgaaaaaca cgaggatctt ttctgtgaat
    21301 aaaaacgggt attttattcc cctcaaaggt aaataaggta acatggacct caggaaaaag
    21361 acactcggta tcctgactgg tgtaataatc ggtataatca tcattttcat ggtcatttcc
    21421 tttaccttct ttttggataa ttaccggaag atggaaaccg gttatgttac cgactattca
    21481 aatctggtcc tgcagaacgt ccagaatgag atgaacaacc tggatatgat cgtcaaggac
    21541 tggggcccgt gggacgacac gtacgcattt gtagcaggag aaaaacccga ttatgtcgcc
    21601 gtaaacctcg ggaaaccggc attccagaac ctccgcatga atttcatcct tatcaccaat
    21661 aatcagggtg atatcctgta tggtgaaggg tatgatctgg aaaagcaggt aataactccc
    21721 ctgcgcccgg atattgttgc agagctttcc cgtgaaggct caacgctccg gaatctggtc
    21781 accacttccc agcccggtgg ttttttaaac cttccccagg ggccggtaat actgtcaacg
    21841 tatccggttc tccatagtga ttacaccggg ccgtcacgcg gcgttgtcat gatcggaaga
    21901 tttctcaccg aagcagaaac agccaaattt gcatacgcga cacaggcaaa ggtttcgatt
    21961 atcccgttgg aacaggcatt gttatctcaa caggatagga atgcgttatc tgaagctgac
    22021 agcacccaac ttcaggtccg gacactcgat gaagataccc ttgaggcaaa ctgggtgata
    22081 caggatatca gcgaaaccgg gaaattcgtc atcagcctga aattaccgcg ggatatttac
    22141 cagcagggaa aaagggacat tctcattttc gttctccttc agctgggtat agggcttgct
    22201 atcgggctga taacaatcag gttcctggat aagcagatac tcaaccgtct ggcgaccatc
    22261 aattctgata tagacgatat taccctgcat aaaacgggca gatcccatat cagggcaacg
    22321 ggaaatgatg aaattgcaca tcttggactt gcaatgaata ccatgattga ccagatcgat
    22381 cgtgacaaga aggaactgaa agcccgtgaa cttcgtttcc gcgaattcgc cgaacaattt
    22441 cctgaggtca tgctggaaac tgataatggg ctaaacctga cgtttatcaa tacgatcgca
    22501 tcagaaaagt ttggttacac acaagatgat cttaagaaaa atgtgaaaat attaaatttc
    22561 attgcccagg aagatcatgt aagggcagag gaaaatttct cgcgggtact gaagggtctg
    22621 cccccctccg ggaacgatta cataatgata aaaaaagatg gcagcaggtt ccctgtgctt
    22681 ctctattctg cgccggtcat catggatgaa aaaattgttg gcgtccggat ttttgcaggg
    22741 gacatcagtg cccgtaaaca gatggagaag aacctgcttg agacaaacag aatattaaat
    22801 ctgctgagcc gggttacgcg gaatgatata tcaagccagc ttttaacgct ctttgggtac
    22861 atggatcttg tagaagaaac ctcctttgag cctgaaaaac ggggttattt taataaaatg
    22921 aaacagtctg cagaaaacat ccggcaacag atttccttta cccgggattt tcaggatatc
    22981 ggcgtcaaag ccccacaatg gcaaaacgtg aaacaggtga tcctgaacgc aaacggcaat
    23041 cacgacacat caagataccg ggtcctgata gatattcaag atgttgaagt ctacgcagac
    23101 aaactccttg aacgggtatt ttataacctt gtcgactacg caaacaaatt cggtggtgcc
    23161 atatcagaaa tccggatgtc agctctggag acccccgaag gtttccggat aagcctcgaa
    23221 catgacggcg ctggcattcc agaggaaaaa aaggaagata tctttaagga agggtatttt
    23281 ggcaatacag aattcggatt gttcctttca cgggaaattc ttgccctgag cggcatcact
    23341 atccgggaaa ccggagatcc ggacaaaggt acacggttct ggattttagt gcccgtaaac
    23401 agatggagaa aaacctgctt gagacaaaca gagtattaaa tctgctaagc cggattacgc
    23461 ggaatgatat atcaagccag cttttaacgc tctttgggta catggatctt gtagaagaaa
    23521 cc
//
LOCUS       DASRLV010000040        12476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_746076,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000040 DASRLV010000000
VERSION     DASRLV010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 12476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12476
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746076"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..175)
                     /locus_tag="VFC43_01550"
                     /pseudo
     CDS             complement(1..175)
                     /locus_tag="VFC43_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617350.1"
                     /note="catalyzes the formation of L-tryptophan from indole
                     and L-serine; frameshifted; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal-phosphate dependent enzyme"
     gene            344..1144
                     /locus_tag="VFC43_01555"
     CDS             344..1144
                     /locus_tag="VFC43_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29907.1"
                     /translation="MPFIYFSQGVASLYPDFTERLMSHLSLRGTEITQQVLKETAAQM
                     HVKHTDEQGHLDITVVNENIKVSYMVERVHKEISKGVMGAIAGAGLGGVIGNIFRKDQ
                     SPGDAITGALGGAAAGGAYEAYQGWEESKNDRTEFAEVLAGTIKEVEDELQYIIQGKT
                     EAKEAILEQGRQKIEEDSAKEGEYRGLLEDVYGQVLAVQEEVEMATCEGSNVTKAKGR
                     IDRAESLYKEAQSSFGRKEYTAIKPKITASRNMVEKAREALSEAQNPQ"
     gene            1367..2038
                     /locus_tag="VFC43_01560"
     CDS             1367..2038
                     /locus_tag="VFC43_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HZK29908.1"
                     /translation="MKHSIVDILFRPGVFFQHTILEKESLKIPALIVLVLGIVSAFYA
                     YLVGGVTGKMMAGLLSGMESIITISSVLGAIIVVFIFWAIWAGVFYLVSSLFKGTGTF
                     KRTLEFTGYGYLPQIFGAILMAIVAIQYLPRVVVPQISAAAVQEDPQILQEAVKTMMH
                     DPAMMEMTQIASIISIVFLLWSANIWISGMRNARQLSERDSALCVGIPVVLYILYMIY
                     TMTGI"
     gene            2039..3202
                     /locus_tag="VFC43_01565"
     CDS             2039..3202
                     /locus_tag="VFC43_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29909.1"
                     /translation="MKNTQFIIIVTFCLIFSTGIGSVTAFQTPESIIAAGNVYVSNIT
                     YDPGTFFTDDTGTVMVYVTNGNSDQSVVVNHVTFGDKNILLTSPSYDSSTNIGPLQNK
                     QFVFSVSAVASEGTYYPTFSLSFRDADSLYSRAVIRVDNTPLELTIIDKPDAFTREKK
                     KTIFVQIANPRKNDVRSVILDVSGTGVTATPDKIFIGNLASGSKSQVNFTVTPELDTP
                     LILTLSYNNGNNPHKVRMYLPITFGIDKMEATPVISNVLVKSEGGIYHINGDVNNAGL
                     ETANTVMVTSLSPAVPQDPYKTYVVGALKPDDFGSFEVTFSANSTTIPLQLSFKDSDG
                     NLYTSVQDVKISALAANSTATGGPSLLFPLIAVVVVIGACIGGWYFYVRRKNK"
     gene            3211..3888
                     /locus_tag="VFC43_01570"
     CDS             3211..3888
                     /locus_tag="VFC43_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK29910.1"
                     /translation="MSDQPVMVFKDVVKVYPLPAGDVTALDGVSFRVDRGEFISIMGP
                     SGSGKSTLLNLMGCLDTPTSGDIFISGTRIGDMSDTDLTNLRRDRIGFIFQYFNLFPL
                     LNIIENVTFPMMLKSQKEVDPEKGREVLRSVQLEDKLFTHTPTELSGGQQQRVAVARA
                     LINNPDILLCDEPTGNLDSKTGDGIMDLMAELNRKGTTIIMVTHDAHIAEYSRRTIRI
                     ADGRIVT"
     gene            3885..5075
                     /locus_tag="VFC43_01575"
     CDS             3885..5075
                     /locus_tag="VFC43_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZK29911.1"
                     /translation="MIFWEIAKRNLRIHLLRSTLAMLGIVIGVVAIASMGILGNSLVL
                     SVSDSLHTVGDSVIVSPYAGGGSGFGQGGGGGGSTSLKISDQQFQQIKRAVAPNIAVP
                     VLQTADIMKLGVGSDTIVAPIYGLNPDDVSKLLNLKAGTYSRSESGCLVGSKFADDHN
                     VQVGSRISIGTDGDKGTLRVTGIIEERGISFDISTDSAIVVTQNWFTTTYNRNDYDTV
                     VVKVNQGVDSAVVKTTIENQMNKRDKVVSVMDSKATLASIFSAFGAVTTFVSAIGGIS
                     MIVAGVSIFNIMMMSVTERIKEIGIMRSIGTQKREVMSMFMYEAAILGIVGSIIGGVL
                     SIIAGYAISTLALNTTKYLFVWSSAIPIIEGICFGIVISLACGIYPAWKAANLSPIEA
                     MRHE"
     gene            5148..6011
                     /locus_tag="VFC43_01580"
     CDS             5148..6011
                     /locus_tag="VFC43_01580"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZK29912.1"
                     /translation="MSSPVLRDPDWNEIWKARQSLHESSKHFDDPSHNWNRRENAERY
                     DSTSRCKYDDRVMTTINGLDINENSRVLDIGAGPGTLAIPLASRVKDVTAVEPGEGMV
                     EILNERMKKEGITNIRTITKRWEDIDLATDLDGQYDVVIASLSLTMEDIGQALQKMDA
                     ASQGSVYLFWFVDMPFWERMYADLWKPLHGTDYHSGPKADCLFGVLYQTGIYPNVEML
                     PLSKDYRFTTQDEMTAFFRRRFNVMNTEQERLLDDYLKPLIRTQGSEIIISGDSTFAK
                     IWWKKFLKKRN"
     gene            complement(6023..7162)
                     /locus_tag="VFC43_01585"
     CDS             complement(6023..7162)
                     /locus_tag="VFC43_01585"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZK29913.1"
                     /translation="MKNLSEKIASVAPSATLAISDKAKKMQRKGIDVISLSIGEPDFD
                     TPKHIKDACIEALNRGETHYAPSNGIPELLNAISEKINKENHFPCTPSQVIVSCGAKD
                     AIYEAMEAVLNPGDETIILTPAWVSYEPCVQIAGGKIVRHEVNEKTFQLDDSLIERVN
                     AKTKMIVVNSPSNPSGAVFNKKSIQLVADLCEDHDIYAMSDEIYEKMIYGREHISLAS
                     MSDMAQRTITINGFSKSYAMTGWRLGYAVAPIEIIRQMSTVQQHSVSQAATFSMWGAV
                     AALKGDQRCVEEMRTEFDRRRKYIISELNRIGYETAPAEGAFYAFINVGGDDIEIAAQ
                     WLEQGHVAVTPGSAFYAPGWIRLSYATSMEKLMEAMGRIRGITGK"
     gene            complement(7159..7578)
                     /gene="ribH"
                     /locus_tag="VFC43_01590"
     CDS             complement(7159..7578)
                     /gene="ribH"
                     /locus_tag="VFC43_01590"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HZK29914.1"
                     /translation="MCDTKPIKLGFVVAEFNRDITYMMEIEGREHAAFLGAEVTECLY
                     VPGAYDMPLAIKKMLQAGKVDAVVTIGCVIEGATQHDDIVVQHAARKIIDLSLEFGKP
                     VTLGISGPGMTRLEANERIDYAKRAVESAIKLVQRLK"
     gene            complement(7637..8098)
                     /gene="ribC"
                     /locus_tag="VFC43_01595"
     CDS             complement(7637..8098)
                     /gene="ribC"
                     /locus_tag="VFC43_01595"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HZK29915.1"
                     /translation="MRVGVADTTFSRVNMGALAIDELKKHASVAIERVTVPGIKDLPV
                     ACKKLIDQRKCDIVMALGMPGGKEKDRLCAHEASQGLIQCQLMTNTHIIEVFVHEDEA
                     EDGNELAWLAEQRTREHAVNAIKLILYPKELEKEAGTGQRQGFADAGPARR"
     gene            complement(8095..9225)
                     /locus_tag="VFC43_01600"
     CDS             complement(8095..9225)
                     /locus_tag="VFC43_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HZK29916.1"
                     /translation="MEKELLLMLPGPVPMPERVRYAMIRQAINHRSAEFGAVYADCVR
                     VLKTAFGTTNDLFVISGSGTAGMEAAVANFGRDKEIACLVNGKFGERLFKISQRYGKA
                     YEIGSDWGTPLNLAALEERLEMGAQVVTLVHNETSAGIKNPAKEVGRLAKKHDAIFLM
                     DGITSIGGDVVEADTWGADVAIVGSQKCLAAPAGLAGISVSNRAWEGLCKNPPYYLDL
                     AAYKKSAANKPMETPYTPAVPLFLALREACLIIEEEGMPSRIARHQKMASAVRAAVKT
                     WGLSLFPKIDNSHEYSNTVTAVSYPTGVKDDEMRASIKKMGIVIAGGQDHLKGKIFRI
                     GSMGAVSAPEILATLAATQYALRKLGFKVEGDGVGAACEVLG"
     gene            9281..9673
                     /locus_tag="VFC43_01605"
     CDS             9281..9673
                     /locus_tag="VFC43_01605"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HZK29917.1"
                     /translation="MADVAIISGSASDAAITDKVKRVLDANNVSYDAQILSAHRDPDK
                     LDAYIKASTCKVYIAIAGLSAALPGVIASKSDKPVIGVPVSGTLNGMDSLLSIAQMPK
                     GVPVACVGVDNGDNAAWLAIRILNLSKC"
     gene            complement(9678..10424)
                     /locus_tag="VFC43_01610"
     CDS             complement(9678..10424)
                     /locus_tag="VFC43_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HZK29918.1"
                     /translation="MNQDIDRQLQIHATSFGADYFGVADLTPARDFIHKQGGEWVSRY
                     PRGIVIGMRLQDSLVDLLPEDDKAAAILYKHNSYDVVNQSLDQIALRVANMLQHAGYG
                     AFPVPASKRTDDEHICGVFSQKLAAHLAGLGWIGKSCLLVTPDHGPRVRWVTVLTDAP
                     LKPTGFPMEPRCGKCTACVDICPEHAFTGRPFCSEEPREARFDAAVCDRYFKEQEKKQ
                     GVAVCGLCLWVCPHGIKTDAGPGKRLYKTR"
     gene            complement(10566..11312)
                     /locus_tag="VFC43_01615"
     CDS             complement(10566..11312)
                     /locus_tag="VFC43_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZK29919.1"
                     /translation="MTLDEFFYQVFEFLPRQGPGCTGATRKVFSYLPPLSKEAKILDV
                     GCGSGSQTRDLAALTSGTIIAVDNHQPFLDSINAWALKAGLEGRIRIHCASMDALLFE
                     PGQFDLIWSEGAIFIMGFFEGLQVWKPLLKKGGYMVVSDAAWFEPEPPQELVQWWEKE
                     GYIPKTEDQLKEQIRNAGLRLLATYRLPEAGWWDNYYVPILARIAELKKTHGTDPACA
                     AILDSLGVEAEMYRKYKQFYGYTFFVMQNP"
     gene            complement(11497..>12476)
                     /gene="thiC"
                     /locus_tag="VFC43_01620"
     CDS             complement(11497..>12476)
                     /gene="thiC"
                     /locus_tag="VFC43_01620"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HZK29920.1"
                     /translation="KKILAFDTTVGTVPVYEAVRRAGNAADVDADLLFRVIREHCQQG
                     VDFLTLHCGVNRQALAALRSDPRVMGVVSRGGSFHCAMMMQRSEENPLFAEYDYLLEI
                     LAEYDVTISLGDGMRPGCLQDAEKLAKSVEYINLGTLAKQALAKGVQRMIEGPGHMPL
                     DQVGYNVRMIKEITDHAPLYLLGPLVTDIAPGYDHIVAAIGGATACLNGADFLCMVSP
                     SEHLALPDVADIIEGTRVAKIAAHIGDTVRKPEGWRMEREVQMADARHELDWDEQFRL
                     ALYGDHARKIHERDGETETCSMCGDLCAVKIVNELFGTGKKKGKKKTSE"
BASE COUNT         3059 a         3161 c         3038 g         3218 t
ORIGIN      
        1 gtggttactt cctgtcgtat aagctccatt ggaaaaatgg cagcgagatc ctcaggacca
       61 ataggtttct gggtcttggg gtggagaggg ggatcaagcg gtgatttcag gtctgcctga
      121 acattgtacc actttttggc atctgctcct catccagaag gatttttgtc tgcatacata
      181 gtcattgcgt tgtataaata tatacattat tgtacacatt tgattttttc ggatgtttat
      241 tatacatcgg aaattaaaaa ctcgattcgg gcgatgtgaa tgaagtaata taaatgatga
      301 aatttatttg tcttataccg gtataacaaa gagaggagag agaatgcctt ttatatattt
      361 cagccagggg gttgcatcac tgtaccctga tttcaccgaa cgcctcatgt cgcacctttc
      421 actcagggga accgaaataa cccagcaggt acttaaggag accgctgccc agatgcacgt
      481 aaaacataca gacgaacagg gtcatcttga tatcaccgtc gtaaatgaaa atattaaagt
      541 ttcctatatg gtcgagcgtg tacataagga gatttccaaa ggcgtgatgg gtgcgatcgc
      601 tggtgctggt ctggggggcg tcattggtaa cattttcagg aaagaccaga gtcctggcga
      661 tgcaataacc ggtgcactcg gcggagctgc ggctggggga gcatatgaag cgtatcaggg
      721 atgggaagag tctaagaacg accggacaga atttgccgaa gtacttgctg gaaccatcaa
      781 agaggtggaa gatgaactgc aatatataat tcaagggaaa acagaggcca aagaggcgat
      841 actcgaacaa gggaggcaaa aaattgaaga ggattctgca aaagaggggg agtatcgtgg
      901 attactggaa gacgtctatg ggcaggtact tgctgtgcag gaagaagttg agatggcaac
      961 gtgcgaaggc tccaatgtga caaaagccaa gggacggatt gaccgggcgg aaagtctgta
     1021 caaggaggct caatcttcat ttggcaggaa agaatacacc gcgataaaac ctaaaatcac
     1081 cgcttcccgg aacatggtgg aaaaggcgcg tgaagcgctc tcagaagcac agaatccgca
     1141 ataaaatttt tgcgtttgac gaatcatacc gaatcaggag ttatgaatga actctcgatt
     1201 gaacctgtcc tggtctgaaa aaagcaatct tgagatcgga cccgggaaca acccgttttg
     1261 acattcgttc caaattatta atatcatcaa gagaaaatga gttatcaagt tgttatttct
     1321 gcaacaacaa aacagatcca tgaaaggggg aaagggagat gatcagatga aacattcaat
     1381 cgttgatatt ttattcagac caggggtgtt cttccagcac accatcctgg agaaagaaag
     1441 tctcaaaatt cccgcactta tcgtattagt gctggggata gtctccgcat tttatgctta
     1501 tttggtcggc ggagtaacgg gaaagatgat ggcaggattg ttatccggaa tggaatcaat
     1561 aatcaccatt tcttcagttc tcggtgctat aatagtggtc ttcatcttct gggcgatatg
     1621 ggccggtgtt ttctacctgg tatcgtcatt attcaagggt accgggacat ttaaaagaac
     1681 acttgaattc accggctacg gatatcttcc tcagattttc ggtgcgattc tgatggccat
     1741 cgttgcaatc cagtatctcc cgcgagttgt tgttccccag atttctgcag ctgcagttca
     1801 ggaagatccc cagatactcc aggaagcagt caagaccatg atgcatgatc ctgcaatgat
     1861 ggagatgacc cagatcgcat caataatttc aattgtattt ctcttatgga gtgcaaatat
     1921 ctggatctcc ggcatgagaa atgcccggca gctttcagaa agggactccg ctctttgtgt
     1981 ggggattccg gtcgtactct atattctcta tatgatctac acaatgacag ggatataaat
     2041 gaaaaacaca caatttatca taatcgtcac cttctgcctg atttttagca cagggattgg
     2101 gtcggtcacc gcattccaga caccggaatc cattattgct gccggtaatg tctatgtgtc
     2161 caatataacg tatgatccgg gaaccttttt taccgatgac acagggacgg tcatggtata
     2221 tgtcaccaat ggtaattctg accagagtgt ggtcgtaaat catgtgactt ttggtgacaa
     2281 aaatatcctg ctcacgagcc cgtcttacga ttcctcaaca aatatcggcc ccctgcagaa
     2341 caagcagttt gttttttccg tttcagctgt tgcttcggag gggacctatt acccgacatt
     2401 ctcactgagt ttccgcgatg cagacagcct ttattcccgt gcggtaataa gggtggataa
     2461 cactccactc gaactgacca ttatcgataa acctgacgca tttaccaggg aaaagaaaaa
     2521 gaccattttc gtccagatag caaatccccg aaaaaatgat gtaaggagcg ttatactcga
     2581 tgtttccggt accggagtta ccgcaactcc ggataaaata ttcataggta accttgcatc
     2641 aggaagcaaa tcacaagtca atttcacggt tactcctgaa cttgataccc ccctgattct
     2701 gacgctatcg tacaacaatg gtaataatcc gcataaggtg aggatgtatc tcccgataac
     2761 atttggaatt gataaaatgg aggcaacccc ggtcatcagt aacgttctgg ttaaatccga
     2821 aggtggtatc taccacatca acggtgatgt aaacaatgcc ggactggaaa cagccaatac
     2881 cgttatggta acttcgcttt cacctgcggt tccgcaggat ccctataaga catatgttgt
     2941 tggtgcgctc aaacctgatg atttcgggag ctttgaagtt acattttctg caaacagcac
     3001 tactattccg cttcaacttt ctttcaaaga ttctgacggg aacctgtaca cttctgttca
     3061 ggatgtaaaa atttctgctc tggcagcaaa ttcaacagca actggaggac cgagtctcct
     3121 cttcccgctc atcgctgtag tggttgtcat tggtgcctgc attggcggat ggtacttcta
     3181 cgtgagaagg aaaaataagt gaccgtctct atgagcgatc agcccgtcat ggtattcaaa
     3241 gacgtagtta aagtgtaccc tctacctgca ggtgacgtta ctgcactgga tggcgtctcg
     3301 ttccgggtcg accgtggcga gtttatctca atcatgggcc cgtccggttc cggcaaatcc
     3361 acgcttctga acctgatggg gtgccttgac acgccgacat ccggtgacat ttttatcagc
     3421 ggaacccgta ttggcgatat gagcgacacc gatctgacta atctcaggcg agacaggatc
     3481 gggtttatct tccagtattt caaccttttt ccactgctca acattataga aaacgtcacc
     3541 tttccgatga tgctgaaatc ccaaaaagag gtggatcctg aaaaagggcg ggaagtgctc
     3601 cggtcagtcc agctcgagga taaattattc acgcatacgc caaccgagct ttctggtgga
     3661 cagcaacagc gggttgccgt cgcacgggca cttatcaata atccagacat ccttctctgc
     3721 gatgaaccaa caggaaacct cgattcaaag accggtgacg ggataatgga cctgatggcc
     3781 gaactcaacc gaaaaggtac gacgatcatc atggtcacgc atgacgccca tattgccgaa
     3841 tactcacggc ggactatacg aattgctgac ggcaggattg ttacatgatc ttttgggaaa
     3901 tcgcaaagcg aaatctccgg attcatcttt tacgctcaac gctggccatg ctcggcatcg
     3961 ttatcggcgt tgtcgccatt gcttcaatgg ggattctcgg caacagcctt gtactttcgg
     4021 tttcagacag tcttcacacg gttggcgaca gtgtgatcgt ttcaccctac gcagggggtg
     4081 gttccgggtt cgggcagggt ggcggaggtg gtggttcgac aagcctgaag atctccgatc
     4141 aacagtttca gcaaattaaa cgagctgttg caccaaacat tgcagttcct gtccttcaga
     4201 ccgcggacat catgaaactc ggtgttggca gcgataccat tgtagcccct atttacggtc
     4261 ttaatcccga tgatgtatcc aagctcctga acctgaaggc ggggacttac agtcgttcgg
     4321 aatccgggtg cctagtcgga tcgaaatttg ccgatgatca caacgtacaa gtcggttcac
     4381 ggatctctat cgggacagac ggggacaaag gaactttacg cgtaaccggt atcatcgagg
     4441 agaggggaat cagttttgat atcagcaccg attctgccat tgttgtgacg caaaactggt
     4501 ttaccactac ctataaccgg aatgattatg atacggttgt agtgaaagtg aaccagggtg
     4561 ttgattccgc ggtcgtaaaa accaccatcg agaatcaaat gaacaagagg gacaaggttg
     4621 tcagcgttat ggacagtaag gcaacacttg cgtcaatttt ttctgcattc ggagcagtca
     4681 ccacatttgt atctgcgatc ggaggaattt ccatgattgt cgcgggcgtt tcaattttca
     4741 atatcatgat gatgtcagtg accgaacgca tcaaagagat aggtatcatg cggagtattg
     4801 gcacccagaa gagggaagtt atgagcatgt tcatgtacga ggcggcaatt cttggcatcg
     4861 tcgggagcat catcggggga gttttgagta taatcgcagg atatgcaata agtacactgg
     4921 cgttgaacac cacgaagtac ctgttcgtct ggtccagtgc gattcctatt atcgaaggga
     4981 tctgttttgg tatcgtgatc agtcttgcct gtggcattta tccggcatgg aaagcagcaa
     5041 acctcagccc gattgaggcg atgaggcatg aataaaatta ctccgttttt gtttttcttt
     5101 ttcattacgt gagaccttta ataccggcca gatgcataca gtgatttatg agttccccgg
     5161 tactcaggga tcccgactgg aatgagatct ggaaggcacg gcagagcctg cacgagtcct
     5221 cgaaacattt cgatgatccc tcgcataact ggaaccgccg ggagaacgct gagcggtacg
     5281 attcaacttc cagatgtaag tatgacgatc gtgtgatgac aacgatcaac ggtttggata
     5341 tcaatgaaaa ttcacgtgtg ctggatatcg gtgccggccc tggtactctt gccatcccgc
     5401 tcgcatcgag agtaaaggat gttactgccg ttgagccggg tgaaggaatg gttgaaatcc
     5461 tcaatgagcg gatgaaaaaa gagggtatca ccaatataag aacaatcaca aaacgctggg
     5521 aagatatcga tcttgcgacc gatcttgacg ggcagtatga tgtcgtgatc gcgtctctct
     5581 cgctcaccat ggaagacatc ggacaagcac ttcaaaaaat ggatgctgct tcgcagggtt
     5641 ccgtgtatct gttctggttt gttgatatgc ctttctggga gagaatgtat gccgatctct
     5701 ggaaacctct ccatggaacc gattaccatt cagggccaaa agccgattgc cttttcggtg
     5761 tcttgtacca gactggcatc tacccaaacg tcgagatgct cccgctcagt aaagattacc
     5821 ggttcacaac acaggatgaa atgaccgctt ttttccggcg caggtttaac gtgatgaaca
     5881 cggaacagga aaggttgctg gatgactatc tcaaaccgtt aatccggaca caaggatcag
     5941 agattattat ttcgggtgat tccacatttg caaagatatg gtggaagaaa ttcttaaaaa
     6001 aacgaaatta atcagtccgg ttttatttcc cggtgatgcc acgtatccgt cccatagctt
     6061 ccattagttt ttccatagac gtggcatacg aaagccggat ccaccccgga gcatagaatg
     6121 cactacccgg agtcacagcc acatgccctt gctccagcca ttgggctgcg atctcaatgt
     6181 catcccctcc aacgttaata aaggcgtaga atgcaccctc tgccggtgct gtttcgtaac
     6241 ctatccggtt cagttcagat ataatgtatt ttcttcgcct gtcaaactca gtccgcattt
     6301 cctcaacgca acgctgatcc cctttgagtg cagccactgc accccacatt gaaaacgtgg
     6361 ctgcctggct gaccgagtgt tgctgaactg ttgacatctg ccggatgatt tctatcggag
     6421 cgaccgcata acccagtcgc cagccggtca tcgcatacga tttcgagaaa ccgtttatag
     6481 tgatggtccg ctgtgccata tcgctcatag atgcaagtga gatatgctcc ctgccgtata
     6541 tcatcttctc gtagatctca tccgacatcg catagatatc atgatcctca cagagatccg
     6601 ccacaagctg tattgacttt ttgttaaaca cggcacccga agggtttgaa ggagagttga
     6661 ccacgatcat cttggtcttt gcattcaccc gctctatcag ggaatcatcg agctggaatg
     6721 tcttttcatt gacttcatgc ctgacaattt tcccgcccgc aatctgcacg cagggttcgt
     6781 aggaaaccca tgcaggtgta aggataatag tttcatcacc cggattcagg accgcttcca
     6841 tcgcctcgta gatagcatcc ttggcgccac acgaaacaat cacctgcgag ggtgtgcagg
     6901 gaaaatgatt ctctttgttg atcttctcac tgattgcatt caggagttcg gggattccat
     6961 tgcttggtgc gtaatgcgtc tcaccccgat tcaacgcttc gatacaggca tccttgatat
     7021 gctttggtgt atcgaaatca ggttcaccta tcgagaggct gatgacatcg atgcctttcc
     7081 gttgcatctt ctttgcctta tcggatatcg caagcgttgc agaaggtgca accgaagcaa
     7141 tcttttccga gagatttttc atttcaatcg ctggacaagc ttgatggcag attcaacagc
     7201 cctctttgcg taatcaatcc gctcgtttgc ctcaaggcgt gtcattccgg gcccggatat
     7261 tccaagagtt accggttttc caaattcaag cgagaggtcg ataattttcc gggctgcatg
     7321 ctgaaccacg atatcgtcat gctgggtagc accctcaata acacagccga tagtaacgac
     7381 tgcatcaact tttccggcct gcagcatctt cttgattgca agtggcatgt cgtatgcccc
     7441 cgggacataa aggcattcag tcacttcagc cccgagaaac gcggcgtgtt ctcttccttc
     7501 aatctccatc atataggtga tgtcacggtt gaattccgca acgacaaaac ccagttttat
     7561 cggctttgta tcgcacatag ttaatcatct cttgtaaatc gaacctgata aatttgtacg
     7621 ctctgttgtt ttctatctaa cggcgtgctg gtcccgcatc agcaaatccc tgacgctgac
     7681 cagtcccggc ttctttttcc agttcctttg ggtaaaggat cagttttatc gcattaactg
     7741 catgttcgcg agtacgttgt tctgcaagcc atgcgagctc gtttccatct tctgcttcat
     7801 cttcatgcac aaatacctct atgatgtgag tattggtcat gagctggcat tgaatgagac
     7861 cttgtgaggc ttcatgggca cagagacgat ccttttcttt tcccccgggc ataccaagag
     7921 ccatcacaat gtcacattta cgctgatcga taagcttttt acaagcgacc gggaggtcct
     7981 tgatacctgg cacagttacc cgctcgatcg cgacactcgc atgttttttc agctcatcga
     8041 tagcgagcgc tcccatgttg acgcgtgaaa aggtggtatc cgcaaccccc accctcatcc
     8101 gagcacctca catgctgccc cgacgccgtc gccctccacc ttaaatccca gtttccttaa
     8161 tgcatattgc gttgcagcaa gggtagcgag tatctctggt gcacttaccg cacccatgct
     8221 cccaatcctg aaaatttttc ccttaaggtg atcctgcccg cctgcgatta cgatccccat
     8281 tttcttaatg gatgcacgca tctcatcatc cttcacaccc gtgggatagg aaactgcagt
     8341 taccgtgttg gaatattcat ggctgttatc gatcttcggg aaaagcgaga gcccccatgt
     8401 tttaactgct gcccgcacgg ccgaagccat tttctggtga cgtgcgatac ggcttggcat
     8461 accctcctct tcaatgatca gacaggcttc cctcagtgct aaaaagaggg ggactgccgg
     8521 ggtgtagggg gtttccatcg gtttattggc ggcacttttt ttgtaggctg caaggtcgag
     8581 atagtaaggg ggattcttac aaagaccctc ccatgctctg ttactgaccg agattccggc
     8641 aagccctgcc ggagcggcga gacacttctg ggatccgaca attgcaacat ctgctcccca
     8701 ggtatctgcc tcaacaacat cacctccgat agaggtgatg ccatccatta aaaagattgc
     8761 atcgtgtttt tttgcgaggc ggccgacctc ttttgccggg ttcttgatac cggcggatgt
     8821 ctcgttatgt acaagcgtaa cgacctgcgc tcccatctcg agccgttcct cgagtgctgc
     8881 aaggttgaga ggggttcccc agtcagagcc tatttcgtat gccttgccgt aacgctggct
     8941 tattttgaac agtcgttcac caaacttccc gttgacaaga caggcgattt ccttgtccct
     9001 gccaaagttt gcaacagcgg cttccatgcc cgcagtcccg gatccactga tcacaaagag
     9061 gtcatttgtt gttccaaagg cggtttttaa tacacgcaca cagtcagcat atacagcacc
     9121 aaactcggcg ctgcggtggt ttattgcctg acgtatcatt gcataccgta cccgttccgg
     9181 cattggaacc gggcccggta gcatcaggag tagttctttt tccatgtaga tctgctcata
     9241 gtattttaat atagacgcaa tataagtttg gatagaagtt atggcagacg tagcgattat
     9301 ttcaggatcc gcatccgatg cggcaatcac cgataaggtc aaacgagtcc tagatgcgaa
     9361 taacgtttct tatgatgccc agatcctctc ggcccaccgc gatcccgaca agctcgatgc
     9421 ctacatcaag gccagcacct gtaaggtata tatcgcaatc gccggtctct ctgcagctct
     9481 gcccggggtg atcgcatcga aatccgataa acccgttatc ggtgtgccgg tgagtgggac
     9541 actaaacgga atggattcgc tcctctccat cgcacagatg ccaaaaggcg tcccggttgc
     9601 ctgcgtgggc gtggacaacg gcgacaacgc ggcatggctg gcgatccgga tcctgaatct
     9661 ttcgaagtgc tgatttttta tcgagtttta tagagtcttt ttcccggccc tgcgtccgtt
     9721 tttatcccat gcggacagac ccagagacag aggccgcaga ccgcgacacc ctgctttttt
     9781 tcctgttcct taaagtagcg gtcgcacacc gccgcatcga accgcgcttc acggggctct
     9841 tcagagcaga acggtctgcc ggtgaacgcg tgctccgggc agatgtcaac gcaggcagtg
     9901 cattttccgc accgcggctc catcgggaat ccggtcggtt tcagtggcgc atcggtaagc
     9961 acagtcaccc accggaccct tggcccgtgg tccggtgtca ccagcaggca gctctttccg
    10021 atccagccca gcccggcaag gtgtgctgcc agtttctggg agaaaacccc gcagatatgc
    10081 tcgtcatcag tcctctttga tgctggtaca ggaaaggcac catatccagc atgctggagc
    10141 atgtttgcaa cccgtagcgc aatctggtca agtgactggt tcacgacgtc atagctgttg
    10201 tgcttgtaga ggatcgctgc tgctttgtcg tcctccggca ggaggtctac aaggctgtcc
    10261 tgcaatctca tgcctatcac gatcccccgc gggtacctgc tgacccattc ccccccctgt
    10321 ttgtggatga aatcacgggc aggagtgagg tccgccacgc cgaagtagtc tgcgccaaaa
    10381 gaggttgcat ggatctgcag ttgccggtcg atatcctggt tcatgaataa ttacctgagg
    10441 gattcaaagg aacatcagga tagtggtcat cggagaggga gttcggcgat atgattttga
    10501 catggaaaaa gtaaggtatc cattcctgat aaggggagta ttactaacgc cgctgactct
    10561 tctggtcacg gattctgcat cacaaagaac gtgtacccat aaaactgctt atacttccgg
    10621 tacatctctg cttcgacgcc gagggagtca aggatggcag cgcatgccgg atcggtcccg
    10681 tgggtctttt tcagttccgc aatccgggca agtatcggga catagtaatt gtcccaccag
    10741 ccggcttcgg gtaaccggta tgttgcgagg agccggagac ctgcgttccg gatctgttcc
    10801 ttgagctgat cctctgtttt gggaatataa ccctcttttt cccaccactg caccagctcc
    10861 tgagggggct ctggttcaaa ccatgccgca tcggaaacta ccatataccc gcccttcttc
    10921 aacaggggtt tccatacctg aagtccctca aaaaaaccca tgatgaaaat tgccccttcg
    10981 gaccagatca ggtcaaactg tcccggttcg aatagcagcg catccatcga agcgcaatgt
    11041 atccggatcc tcccttcaag acctgccttt aatgcccatg cgtttataga atccagaaac
    11101 ggctggtgat tatctaccgc aatgatggtc ccgctggtga gtgctgccag gtcccgggtc
    11161 tgggatcctg aaccacaccc gacatcgagg atctttgctt cctttgagag aggaggtaga
    11221 tatgaaaaca ctttccttgt cgcacccgtg cacccgggtc cctgccgcgg aaggaactcg
    11281 aatacctggt aaaaaaactc atcgagtgtc attatacctc aagggttgga ggggggattt
    11341 agagtgcttt gtggtctccg aggggggagg tctaggtcgc ataccgcact ggtccactca
    11401 accaacaatt cagacttgtt ggggcagagg tctcttgtgc ttcatttttg caaccggatt
    11461 tccaattttt ttctttgggc ctcgttgtca tgagatttac tccgaagtct tctttttccc
    11521 cttcttcttc ccagtcccaa acagctcgtt cactatcttg actgcacaca gatccccgca
    11581 catcgagcat gtttctgttt cgccgtccct ttcgtggatc ttccgggcat ggtccccata
    11641 gagagccagc cggaactgtt cgtcccagtc aagctcatgc cgggcatcag ccatttgcac
    11701 ctcgcgttcc atgcgccatc cctcgggctt ccggactgtg tccccgatgt gggcagcgat
    11761 ttttgctacc cgggtcccct cgatgatgtc cgcgacatcc ggcaacgcga ggtgctcgct
    11821 cggtgagacc atgcacagga aatcggcacc gttcaggcat gcagtagccc caccgatggc
    11881 agccacgata tggtcataac ccggcgcgat atcggtgacg agtggtccga gcaggtacag
    11941 cggggcatga tcagtaattt ccttgatcat ccgcacattg tagcccacct gatcgagcgg
    12001 catatgtccc ggcccttcga tcatccgctg gacccccttt gccaatgcct gttttgcaag
    12061 cgtaccgaga ttaatatact ccactgactt tgcgagtttt tctgcgtcct gcaggcaacc
    12121 ggggcgcatc ccgtcaccca ggcttatcgt cacgtcatac tctgcgagga tctccaaaag
    12181 gtaatcatat tcagcgaaca gcgggttctc ctcgctcctt tgcatcatca tcgcacagtg
    12241 gaatgacccg ccccggctca ccactcccat cacccgcgga tcgctccgta gcgctgcaag
    12301 cgcctgccgg ttcacaccgc aatggagcgt caggaagtct accccctgct ggcagtgctc
    12361 ccggatgaca cggaagagaa ggtcggcatc aacatccgcc gcattaccag cccggcgtac
    12421 cgcttcatag acgggaaccg ttccgacagt agtatcaaat gcaaggattt ttttcc
//
LOCUS       DASRLV010000041         5735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1148495,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000041 DASRLV010000000
VERSION     DASRLV010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148495"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VFC43_01625"
     CDS             complement(<1..698)
                     /locus_tag="VFC43_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZK29921.1"
                     /translation="MHITELEIDNFKSFSKKTKIPFLEGFSVISGPNGSGKSNIIDSI
                     LFVLALSSSRNLRAEKLTDLINLNSGRNTAEVALEFSDGTKIKRRIKRTGNGYYSYNY
                     LNDRLCKQSDIVDHLAKHGIKPHGYNVVMQGDVTRIMEMSDFERRKIIDEIAGVSEFD
                     IKKQQSLSELDIVRERIEREELLLFELTKRVNELKREREHALEYQKWQKNLLFFQNCR
                     AAAQLHEREKELSSL"
     gene            complement(711..1268)
                     /locus_tag="VFC43_01630"
     CDS             complement(711..1268)
                     /locus_tag="VFC43_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29922.1"
                     /translation="MAQSDAKIKYVENELSQKEKLIPMLEISGTGAATEERIVAIEKK
                     VRDIEALVKGLTQELLDLKSIAMKMSKQTEERSRQALRGPPVVQGVQPQPATPTGAPG
                     ASSTVVVRKGVRPSDAPSAPPEPSMDMIMQSDGTMKLEERRGDKNYIVASAGYGRNKK
                     GTSVKQKQGDLIVATEEDKKDPAKK"
     gene            1511..2749
                     /gene="glyA"
                     /locus_tag="VFC43_01635"
     CDS             1511..2749
                     /gene="glyA"
                     /locus_tag="VFC43_01635"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HZK29923.1"
                     /translation="MSYLSKTDPEIADIIEKERLRQVNGLELIASENVVSRAVLEAMG
                     SIMTNKYAEGYPGKRYYGGCEFHDMAENLARNRLKQLFGAEHANVQPHSGTQANMAVY
                     FAYMKLGEKILSMKLNQGGHLSHGSPVSFTGKFYQVAQYGLDPKTEMLDYGAVEEMAK
                     AEKPQIIVCGASAYPRTIDFKTFQSIADGVGAYCMADIAHIAGLVATGLHPTSVGVVN
                     FTTTTTHKTLRGPRGGAIMCNNEYAPAIDKAVFPGMQGGPLMHTITAKAVCFEEALRP
                     SFREYNKQIIKNAKVLAETLMNNGLRLVSGGTDNHLMLLDLTDQGITGLEAETALGKA
                     GITVNKNTIPNETKSPFVTSGLRVGTPAVTSRGMKEAEMRQIGAWIATVVKDINNDAM
                     IKKVQADVTAMSGRFTLYPE"
     gene            2768..3610
                     /gene="folD"
                     /locus_tag="VFC43_01640"
     CDS             2768..3610
                     /gene="folD"
                     /locus_tag="VFC43_01640"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HZK29924.1"
                     /translation="MILDGKKTADKRLEILKEEIEDSGLYQRLATVIAGTDPASQMYI
                     RMKHRACEQVGIGSVSREIAGDATTDDVLQGVAQLNNDPDIDGILIQLPLPKQVDTER
                     VIASVSPDKDVDGFHPFNLGLLFSGKPRFVPCTPKGILTLLNEYKIEVAGTRAVVIGR
                     SIDVGRPMSALLLNADATVTVCHRKTKNLKEELRNADILVSAIGKAHFITAEMVKPGA
                     VVIDVGINQLNGKLAGDVDFAAVKDIASAITPVPGGVGPMTIASLMENTCRAANVRKC
                     GTVL"
     gene            3592..4404
                     /gene="folP"
                     /locus_tag="VFC43_01645"
     CDS             3592..4404
                     /gene="folP"
                     /locus_tag="VFC43_01645"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZK29925.1"
                     /translation="MRDCVINKLKIGGDSPVRLMGVINCSPESFYANSYIPTDFVHKT
                     AVAMIEQGADLIDIGARSTAPNTQAISGTAEAERIDAALKELDGSGITVSVDTMNPWL
                     LDICLKHDIHAFNDISGLTSPVYTQRLAQSGLPAFLMASRNQPGDAVGLDATLAALSG
                     VVDRCEKAGITNYVLDPGIGIWTPFRSVEDDWTLCRHFSEFGKFDRPLLAAISRKTFI
                     GDLLNRYPEDRLAGSLAVTMMLLSRGASVVRTHDVAETLDVMRVFERMVKRS"
     gene            4401..5177
                     /gene="cofE"
                     /locus_tag="VFC43_01650"
     CDS             4401..5177
                     /gene="cofE"
                     /locus_tag="VFC43_01650"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HZK29926.1"
                     /translation="MNSAFEVFGLATGIIAVGDSIADRVVASARQTCGGFEDGDILVL
                     AETAVATAEGNVLHLSDIEPSPQAQDLAEKYRMDPRTVEVVLRESDSVVGGISGFLLC
                     MKHGTLLPNAGVDASNAPPGCVTPLPKNPDQSALSIRNAIYDQCGVKIAVIIADSRTH
                     AMRRGCSGVAIGCAGITAVIDDRGRSDLFGRKLEVTQRAVADNLASAAELVMGEADEC
                     IPAAIIRGFGIPIGDQTGIEAIGAEECLFMGVFRKSAPPL"
     gene            complement(5537..>5735)
                     /locus_tag="VFC43_01655"
     CDS             complement(5537..>5735)
                     /locus_tag="VFC43_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HZK29927.1"
                     /translation="EAGLSCEVIDSFRLHTDIDEKEDLVELLIHGTGKSRAFLEELGF
                     ILSVDKGRVNVERKANKSEDA"
BASE COUNT         1369 a         1482 c         1462 g         1422 t
ORIGIN      
        1 agagatgaga gctctttttc ccgttcgtgg agctgggctg cggcccggca gttctggaaa
       61 aacaagagat tcttttgcca tttctggtac tcgagagcat gctcccgctc acgtttgagc
      121 tcattcactc tcttggtcag ctcaaacagg agcagctctt cccgttcgat ccgttccctg
      181 acgatatcaa gttcggacag ggactgctgc ttttttatat caaactctga aacacccgca
      241 atctcatcaa taatctttcg tcgctcgaaa tcactcatct ccatgatgcg ggtgacatct
      301 ccctgcatga cgacattgta gccatgaggt ttaatcccgt gttttgcgag gtgatcaaca
      361 atgtcgctct gcttacatag ccgatcatta aggtaattgt agctgtagta accgttgcct
      421 gtacgtttga ttcgtctctt gatttttgtc ccgtcggaaa attcgagcgc aacttctgca
      481 gtattcctgc cggagttgag attgatcagg tcggtaagtt tctcagcccg aagattccgt
      541 gaactcgaca acgcaagcac aaaaagaatt gaatcgataa tgttgctttt tcccgaaccg
      601 ttaggcccgg atataactga aaatccctct aaaaagggga tttttgtctt tttagaaaaa
      661 gacttgaaat tgtcaatctc cagttccgtg atatgcaaag atccggctcc ttacttcttt
      721 gcaggatcct ttttgtcttc ctctgtggca acgatgagat cgccctgttt ttgcttgacg
      781 gacgttccct tcttattacg cccgtatcct gccgatgcaa cgatgtagtt tttgtcccca
      841 cgccgttctt ccagtttcat ggttccatct gattgcatga tcatgtccat tgagggttct
      901 ggcggtgctg acggggcatc agacgggcgg actcccttgc gcacaacaac cgtgcttgaa
      961 gctcccggag caccggttgg ggtggcaggt tgtggttgta ctccctgaac aaccggtggt
     1021 ccccgcagag cctgcctgct gcgttcttcg gtctgtttgg acattttcat tgcaattgac
     1081 ttgagatcga gaagctcctg ggttaacccc ttgacaagcg cttcaatatc tctcactttt
     1141 ttttcaatcg ctacaatacg ctcctcagtg gcagctccgg ttcctgaaat ttccagcatt
     1201 ggtatcagtt tctccttttg tgatagttcg ttctcaacat atttgatctt tgcatcagat
     1261 tgcgccatac gataggatac tgtactggtg cggcgcactc cttataaata atagtagtaa
     1321 tcgccaaata aggaaaaaag gtttgtgctc tggggtatca aaaccccgga ggttgtgcat
     1381 tatcctgcat gttttaaccg ggattcacgc gcaccctgat taaaaaagcc gatgcaaccg
     1441 cataccctct taacccaccc gccagtattt tggaaaaggt attacctgat tgaagcgaac
     1501 ccttttccat atgtcctatc tgtctaaaac tgatccagaa atagcagata tcatcgagaa
     1561 agagaggctc cgccaggtga atggcctgga actgattgca tccgaaaatg ttgtttcccg
     1621 tgcggttctt gaagcaatgg gatccatcat gaccaataaa tatgccgagg gctaccctgg
     1681 caagcgatac tatgggggat gcgagttcca cgatatggcg gagaaccttg cccgcaaccg
     1741 gctgaagcag ctcttcgggg cagagcatgc gaacgtccag ccgcactcgg gcacccaggc
     1801 aaacatggct gtctattttg cgtacatgaa actgggagag aagatcctga gcatgaaact
     1861 caaccagggt ggtcacttat cccatggctc cccggtcagt tttaccggga aattctacca
     1921 ggttgcacag tacggcctcg atcccaaaac ggagatgctc gattacggtg cagttgaaga
     1981 gatggcaaag gcggaaaaac cgcagatcat cgtctgcggt gcctcagcgt acccccgcac
     2041 catcgacttc aagacattcc agtcgattgc tgacggtgtc ggtgcatact gcatggcaga
     2101 cattgcccat atcgccggtc ttgtcgccac gggcctccac cccacctctg tcggcgtggt
     2161 caactttact accaccacca cccacaagac cctccgcggg ccccgtggtg gcgccattat
     2221 gtgcaacaac gagtatgcac cggcaatcga taaggcagta ttccccggca tgcagggagg
     2281 tcccctgatg cacacgatta ctgcaaaggc cgtctgtttc gaagaagcgc tcaggccctc
     2341 tttcagggaa tacaataaac agatcatcaa aaatgcaaag gtccttgccg agaccctgat
     2401 gaacaacggt ctcaggcttg tctcgggcgg tactgacaac cacctcatgc tccttgatct
     2461 cacggaccag ggtattaccg gtctcgaagc agagacagcc ctgggtaagg ccggcattac
     2521 ggtcaacaag aacaccatcc cgaacgagac taagagcccg tttgtcacca gcggtctgcg
     2581 tgtcggcacg ccggcggtca cttcccgggg tatgaaagaa gctgagatgc gccagatcgg
     2641 tgcctggata gcgaccgtag taaaagatat caacaacgat gctatgataa aaaaagtgca
     2701 ggctgatgta accgcaatgt cgggcagatt caccctgtac ccagaataga ttagtcagga
     2761 acctcccatg atccttgacg gaaaaaagac tgcagacaag cggctggaaa tccttaaaga
     2821 ggagattgag gactctggcc tgtaccagcg acttgcaacg gtcatcgcag gaacagatcc
     2881 ggcatcacag atgtatatca ggatgaaaca ccgtgcctgc gaacaggtag gcataggatc
     2941 tgtcagtcgg gagattgccg gtgatgcaac aaccgacgac gtgttacaag gtgtcgccca
     3001 gctgaataac gatcctgata tcgatggcat ccttatccag cttcccctcc caaaacaggt
     3061 tgataccgaa cgggtaattg cgtctgtcag cccggataag gatgtcgatg gatttcaccc
     3121 gttcaatctg ggccttcttt tttccggaaa gccccggttt gttccctgta cgccaaaagg
     3181 gattctgacc cttctgaatg aatacaaaat tgaggttgcg gggacccggg ctgtcgtaat
     3241 cgggcgcagt attgatgtgg gtcgtccaat gtccgcatta ctcctgaatg ccgatgccac
     3301 cgttacggtt tgtcaccgta agacaaaaaa tctcaaagag gaactcagga atgcagatat
     3361 ccttgtatcc gcaatcggca aagcgcactt tattaccgcc gaaatggtca aaccgggtgc
     3421 ggtcgtcatt gatgtgggaa tcaaccagct caacggtaaa ctggcaggcg atgtggactt
     3481 tgcagcagtc aaagatatcg cttccgctat caccccggtg ccagggggag taggcccgat
     3541 gacgattgcg tccctgatgg agaatacctg tcgtgcagcg aatgtgagga aatgcgggac
     3601 tgtgttataa acaaactaaa aatcggcggg gattccccgg ttcgcctgat gggcgtgatc
     3661 aactgcagcc cggagtcttt ttacgcaaat tcctatatcc ccacggattt tgtgcataaa
     3721 actgcggtcg cgatgataga acagggtgca gatctcatcg atatcggtgc acggagcact
     3781 gcacccaata cccaggctat aagcgggact gccgaagcgg aacgtattga cgctgcgctc
     3841 aaagaactgg atggcagcgg aatcacggtc tctgtagaca ccatgaatcc ctggctgctt
     3901 gatatctgcc tgaagcatga tatccacgcc ttcaatgata tttcagggct gacatcaccg
     3961 gtatataccc agcgacttgc ccagtcaggc cttcctgcat tcctcatggc atccagaaac
     4021 cagccggggg atgccgttgg gctggatgcg acccttgcag cactttccgg cgtagtagat
     4081 cgctgcgaaa aggcgggcat tacaaattat gtccttgatc cgggaatcgg gatctggacc
     4141 ccgtttcggt cggtagaaga tgactggacg ctctgccggc atttttctga attcgggaaa
     4201 ttcgaccgcc ctcttctcgc tgcaatatca agaaagactt ttatcgggga tcttttaaac
     4261 cggtaccccg aagatcggct ggccggcagc cttgcagtca ccatgatgct gctctccaga
     4321 ggagcatcgg tagtccggac acatgacgtt gcagaaaccc ttgatgtgat gcgggttttc
     4381 gaacgcatgg tgaaacgctc atgaacagtg cgtttgaggt tttcgggctc gcgacgggaa
     4441 taattgccgt tggcgattcg atcgccgatc gtgtcgttgc atcagcgagg cagacgtgtg
     4501 ggggttttga ggacggcgat atcctcgtcc ttgctgaaac ggctgtcgct actgctgaag
     4561 gaaatgttct gcacctctct gacatcgaac cctctccgca ggctcaggat ctggcggaaa
     4621 aataccggat ggatccccgt actgtggaag tcgtactgcg ggaaagcgat tctgttgtcg
     4681 ggggtatctc cgggttcctg ttatgcatga aacacggtac cctgctgccc aatgccggag
     4741 tggacgcatc aaatgctccg ccgggatgcg ttacacctct tccaaagaac ccggaccaga
     4801 gtgccctttc catcaggaac gccatctatg atcagtgcgg ggtgaagatt gctgttatca
     4861 tcgctgacag ccggacccat gcaatgcggc gcgggtgcag cggggttgca atcggttgcg
     4921 caggaattac ggcagtcatc gatgatcggg gaagaagtga cctgtttggc agaaaacttg
     4981 aggttaccca acgggcagtt gcagacaacc tcgcttcggc ggctgaactg gtcatgggcg
     5041 aggcagacga gtgcataccc gcggcgatta tcaggggatt tggcataccc atcggtgatc
     5101 agaccggtat tgaagcaatt ggtgcagaag agtgcctgtt catgggggta ttccggaaaa
     5161 gtgcacctcc cctgtaaatt caggggacca gtaatcaggg agtcgatagc aaaatcattt
     5221 cgttcaataa tccctgcaag agactatttg gacacaactc ccatcttttt ggtcatccac
     5281 ccctcacggt tctcctcttc aggatttgcg gggggtaatt ttacaggtat tcggcaacaa
     5341 ccaggttcag cacgcggtcg ccaagaaatt cataccgctg ccactcattg tttgagtatg
     5401 ccatttttcc gatggataat tatccggatc ggaagagccg gcattgaaag tttttataaa
     5461 ttccctgatg gtctggcaca tttgtgtgtg agccgagagg ttgtgcttgt aataattttt
     5521 tttgccggtt gttcattcag gcatcttctg atttattcgc ctttcgctcc acatttaccc
     5581 gccctttatc caccgagagg ataaatccaa gttcctcgag gaatgcccgg ctctttccgg
     5641 tcccatgaat caacagttcg acaagatctt ctttctcatc gatatccgta tgaagcctga
     5701 atgaatctat cacttcgcag gaaagccctg cctcg
//
LOCUS       DASRLV010000042         3234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_747094,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000042 DASRLV010000000
VERSION     DASRLV010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747094"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..512
                     /locus_tag="VFC43_01660"
     CDS             6..512
                     /locus_tag="VFC43_01660"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide adenylyltransferase"
                     /protein_id="HZK29928.1"
                     /translation="MRRGFYIGRFQPYHNGHQSVLEHIADDVDEIVIGIGSAQLSHLT
                     DNPFTAGERVLMITRALASLGCPFYVIPIEDIKRNALWVAHVLSMTPPFDLCYSSNPL
                     VVQLFNEAGIKVQSPAMYERETLCGTEIRRRMLMGEPWKSLVPPEVIRVIKEIDGVER
                     LVQISQKD"
     gene            564..1850
                     /locus_tag="VFC43_01665"
     CDS             564..1850
                     /locus_tag="VFC43_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29929.1"
                     /translation="MYKFIKDLLKKKEPEKLVLPLDAVPALLDDREKALRLVLETETH
                     LPIQNIRNASAQLQHIVNGIAGAEHDPAIHPKLKSIAKNSLPLFVRAMNASLKRDLPE
                     DIEEFYAASVESVKGCLNSTRGQGRYLQAVFPEEMKAVKTGIDAIGHEINTITASLAR
                     FQKQKALIGAARSLHDALYDLRIDARKSLEKDLRIANRIRDTTDRISSIDKDLAELSS
                     DARMQEVKEKKLSLSEVEMKRDERIRTYTALSMTASHVFRKAEKIATKQKLSPEISNL
                     RHTIDLLSDHTAPDTKELASALITAFPVAQRMIDSGDIILKNKEERAIFSDTGKFSND
                     IFSICKEIMIQEMAWKSAQDELASHPVLMRINALDREKTQLVSMLNKEHQSKKDLAEW
                     SERTVVKIPAIADELNQKLVDIVGGSVQLQADDLSQ"
     gene            complement(2054..2248)
                     /locus_tag="VFC43_01670"
     CDS             complement(2054..2248)
                     /locus_tag="VFC43_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VapB family protein"
                     /protein_id="HZK29930.1"
                     /translation="MPRAGEGRSIRIDPEVYEALLMLKRGNMTFSDVIAQMLRDCYGW
                     SDEFSANQSNLEDFVDINAR"
     gene            complement(2385..3146)
                     /locus_tag="VFC43_01675"
     CDS             complement(2385..3146)
                     /locus_tag="VFC43_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZK29931.1"
                     /translation="MGAIVFSDVHADSVAIRALGACIKTPLFKKEIGQTDMLVNLGDL
                     VHRGDRPLETLETIYELGNEYRLVSVMGNHDHAYVNRLLVSGSDPASTYRHEILRGSP
                     LLSIFDTMPMEWSHCGMLCVHGGPLELGNQTLRLKCWQRLSYDSGDSFAGFHYTAPMA
                     FETLEAQGLKHMCCGHQHHNICCRKTEHGITQKMIDFEPLAAENDENKYRIEVAKIPL
                     DMPTLLRVGGCHGDYPEFAYTDFSTFSFIRINLRN"
BASE COUNT          907 a          775 c          761 g          791 t
ORIGIN      
        1 aaacaatgag acgcggtttt tatatcgggc ggtttcagcc gtaccataac gggcaccagt
       61 ctgtgcttga acatattgca gatgacgtgg atgagattgt tattggcata ggaagtgccc
      121 agctttcaca ccttacggac aatcctttta ccgcgggaga gagggtcctg atgatcaccc
      181 gcgcgcttgc atcactcggt tgcccgtttt acgtcattcc tatcgaagat atcaagcgta
      241 acgcactctg ggttgcccat gtcttatcga tgacaccccc gtttgacctc tgttactctt
      301 caaatcccct ggttgtccag cttttcaacg aggccggtat caaagtacag tcgccggcaa
      361 tgtatgagcg cgagacactg tgcggcacag agattcgacg gcgtatgctc atgggagagc
      421 catggaaatc tctcgtgccc ccggaagtca ttcgggtaat caaagagatc gatggtgtag
      481 agcggcttgt acagatttcc cagaaagatt aacgatgaac atggcgacat ttccggaaat
      541 accttcaaaa cctggtgaaa aaaatgtata aattcatcaa agacctctta aaaaaaaagg
      601 aaccggagaa gctcgtcctt cccctggatg cagttcccgc attgcttgat gatcgtgaga
      661 aggcgttgag gcttgtactg gaaactgaaa cacatcttcc catacagaat atcaggaatg
      721 catcggcaca gttacagcat atagtgaacg ggattgcggg tgctgaacat gatccggcaa
      781 tccaccccaa gcttaaaagt attgcaaaaa attcccttcc cctttttgta agggcaatga
      841 atgcctcgtt aaaaagggac cttcccgaag atattgagga gttttatgct gcctcggttg
      901 aatccgtgaa aggctgcctc aacagcaccc gggggcaggg gaggtacctc caggccgtgt
      961 tccccgaaga gatgaaagca gtgaagaccg gcatcgacgc tatcgggcat gaaataaata
     1021 ccatcaccgc gtccctggca aggttccaga aacagaaagc attgatcggt gccgcccggt
     1081 ccctgcatga tgccctgtac gatctcagga ttgatgcccg gaaatctttg gaaaaagacc
     1141 tgcggatagc caaccgtatc agggatacta ccgacaggat aagcagcatt gacaaagatc
     1201 tggcggaatt gtcttccgat gcaagaatgc aggaggtaaa ggaaaaaaaa ttgtccctgt
     1261 ctgaggtgga gatgaagcgg gacgaacgaa tccggaccta tactgctctt tcgatgactg
     1321 catcccatgt attccggaaa gcagaaaaaa tagccacaaa acaaaaactg tcaccggaga
     1381 tttccaacct ccggcacacg attgatctcc tttccgatca tacagctccg gacacaaagg
     1441 agcttgcatc cgctctcatt actgcttttc ctgttgccca gcggatgata gattctgggg
     1501 acataatttt aaaaaacaag gaagaacggg caattttttc cgataccgga aaattcagta
     1561 acgacatttt ttccatctgc aaggaaatca tgatccagga gatggcatgg aaaagcgcac
     1621 aggatgagct ggcatctcat ccggtactca tgcggatcaa tgcccttgac cgggaaaaaa
     1681 cccagctggt atccatgctg aacaaggagc accagtccaa aaaagatctc gccgaatgga
     1741 gcgagagaac agtggtaaaa attcccgcaa ttgctgatga gctcaatcaa aaattagtag
     1801 atattgttgg gggaagcgtg caacttcagg ctgatgacct gtcccagtga gagggtaaga
     1861 taccgggatg atgtgtggtc aatatatctc attttcattt tcttccattt ccatgaacaa
     1921 ttcatcgata ttttcgatga cccatctgat tttatcctga ttgagatcct cggtaaatat
     1981 cctgttcatt gaatccaaca ccgtatctta gtacgataag gcagaccctg tttctccggg
     2041 ccctgtgagg aaattatctg gcattgatgt ccacaaaatc ctcaagattg ctctggttgg
     2101 ctgaaaactc atcagaccac ccgtagcagt ccctgagcat ctgtgcaatg acgtcagaaa
     2161 acgtcatatt cccacgtttt aacatgagaa gcgcctcata cacttcggga tctattcgta
     2221 tagaacgccc ctcgcctgca cgaggcatat ttaccacttt gtggtcacac tatttaaaac
     2281 tatcccaaaa aacctgtctg actttatatg caaggggaaa aacagtcaca aattggagag
     2341 atcctgatcg ggtgtttttg tgtttttctg ctactcgaga ttcattaatt ccggaggttt
     2401 atacgaatga aggagaatgt tgagaaatct gtataggcaa actcggggta atcaccgtgg
     2461 caccctccca cacgcagcag cgtcggcata tcgagaggaa tttttgccac ctcaatgcgg
     2521 tacttatttt catcattttc tgcagcaagt ggctcaaagt caatcatttt ttgggttatg
     2581 ccatgctctg ttttcctgca gcagatatta tggtgctgat gaccgcaaca catgtgctta
     2641 agaccctgcg cctccagcgt ttcaaacgcc atgggggcgg tgtaatgaaa tccggcaaag
     2701 gaatctccgg aatcataaga gagcctctgc cagcacttga ggcggagggt ctggttgccc
     2761 agttcaaggg ggcccccgtg gacacacagc atgccacaat gcgaccattc catgggcatc
     2821 gtatcaaaaa ttgacaggag aggtgatccc cgcaatattt catgacgata cgtacttgcc
     2881 ggatcactgc cactgactaa aagccggttc acatatgcat gatcatgatt acccatcact
     2941 gatacgagcc gatattcatt tccgagttca taaatggtct caagggtttc cagggggcgg
     3001 tcgccccggt gaacgagatc cccaagattg accagcatat ccgtctggcc gatctctttt
     3061 ttaaacaaag gggtcttaat gcatgctcca agcgcacgga ttgcaactga atctgcatga
     3121 acgtcagaaa agactatcgc acccataatg gtggcaaagt attggctctt ctttctctta
     3181 aaaaccgtag atagagcagg ttataccagt aaacccggaa gaataaagat acgt
//
LOCUS       DASRLV010000043        15235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_664531,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000043 DASRLV010000000
VERSION     DASRLV010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 15235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15235
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664531"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="VFC43_01680"
     CDS             complement(<1..840)
                     /locus_tag="VFC43_01680"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HZK29932.1"
                     /translation="MNYKIIALLVVCVAMVAAAGCTGLQSTSTGRSSISQDASNGGSL
                     AEKTAYSGPASMPVPTMAPTQSGPGTTGIDTKIIKTAYLTIEVKDVPGAVEILRTLAA
                     QKGGYISSTNIQKNYNDRLSGSVTLRVPAPEFDATLAGVRALGTVKSASTQGQDVTEE
                     YIDIQAQKTSYQNQLAQYNEIMKRAVKVEDVITVQQQIDRVQTELNRLEGRLKYLNSR
                     IDLSTITVNLEEPEPVGGETGHNFVSTINEGIAGFIGMIDALIIIFFTLLPLVILGGA
                     AYAV"
     gene            1020..1622
                     /locus_tag="VFC43_01685"
     CDS             1020..1622
                     /locus_tag="VFC43_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZK29933.1"
                     /translation="MAEYSIPEKPEGAAELEKKGVEDLVHALRDSTDPMVRQYSAYLL
                     GKAQNPRAIQPLVEALGDFDKSVREQATLALSAIGKAAIAPLSAAMKEPKWETRYRAA
                     EALGKIADEKAVQPLIQALKDNRDHVRYMAAKGLREFGNSEAVDPMIILLKDENRYVR
                     MMAIRALGAIGGKKVHDALKQAFESESDEKVKEAITEAMK"
     gene            1926..4022
                     /locus_tag="VFC43_01690"
     CDS             1926..4022
                     /locus_tag="VFC43_01690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK29934.1"
                     /translation="MILLILIVGALVVVNDYYNTKNIFDKNSQHLRQMTEQDIIVIIK
                     LTDESYNLYDSSLNEQMRRGFDSVLESYKQSSGNPSLMNLTDLKMNLGEDFDIYIINE
                     SGVIEFTTYVPERGMDFKKIPYFFAYLTKIRNSEGFFPDRIVEDQMGTGKTRKFAYMP
                     TPDHRYVLELGYSRTSFSGERASIRYKNAIDRIASFNPYIERVRIFNSMGKIADNTSE
                     PVDEPTRLLLAELMQERQDHTVKLPEAGKSVKYLFIDLKNELYGSDASRIVEITYDDA
                     MLERAFLEHVKFLLVVFIMALTVGICAAFVLSRYLIKPISGIVKDVNRISDGELDLKI
                     ASTHVTEFQNLEQSINTMVSSLKKAFREVQDEKSFQLEMVDQLPVAVFMKGVQDGKYV
                     FWNKASEEIFDLTSSEVIGRTDKDLFSEHMVSVIEQEDEEACLKRVMILNKKIVHKSR
                     GQRIIHMILVPIFDSAKTVRYILGIGEDLTGETLNMKTDLLFSITRRDILDQLSDIVS
                     YLERAQLKISHEATQTFFDKTLESVESIRNHMAFVSSLQAMGITTPTWQSVMKSFWSA
                     VALLPAKKIDIRADMDDFELFADPLLSRVFYNLLVNSLQHGNPQMTKIRFQARQVGDS
                     LILSYEDNGMGIPADEKEKIFEYGYGKRTGLGLFLARELLGYTGMTITETGVPRMGAR
                     FEIVVPKGKFRKVTSE"
     gene            complement(4317..5960)
                     /gene="ilvD"
                     /locus_tag="VFC43_01695"
     CDS             complement(4317..5960)
                     /gene="ilvD"
                     /locus_tag="VFC43_01695"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HZK29935.1"
                     /translation="MRSDEIKSGYQRAPNRSLLRSLGVTDQEMGLPFIGIANAYNNIV
                     PGHVHLRQLAEKVREGIAAGGGVPFEFGVIGICDGIAMGHEGMRYSLPSRENIADSVE
                     LMVQAHRFDGLVCIGTCDKIVPGMLMAAARCNIPSVILTGGPMLSGYQDGRDLSLIDI
                     FEGVGKVAAGTMSEPELCELEACAMPGCGSCQGLYTANTMACMTEAMGMSLSGCAAIP
                     AVDAGKLRIARETGEAIIPLVKKQVRPRDIITKKSLRNAIRVDMALGGSTNTVLHLMA
                     IAVEADVPLSLEDFNTLADGVPHICHMQPSGPHSMQTLYRAGGIPAVLKRLEKYLEDS
                     PTVSGNKILQIARSADVKNEEVIRSTRDPISPAGGLKILFGSLAPDGAVVKCAAVPKK
                     MWKHAGPARVFDGEQQAMKAILSREIKEGDVIIIRYEGPRGAPGMPEMLSPTSALMGF
                     GYTRVVLITDGRFSGGTRGPCIGHVSPEAAIGGPIALVKDNDRIEIDLFLKKIDLLVD
                     ETTLSKRKKTLKPLQKSLSGVLARYAKTVGQANLGAVQK"
     gene            complement(6040..7377)
                     /locus_tag="VFC43_01700"
     CDS             complement(6040..7377)
                     /locus_tag="VFC43_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HZK29936.1"
                     /translation="MNGVPRIVIAGTHSGCGKTTIASGIMAALTARGLKVQPFKAGPD
                     FIDPSYHTRICHRPCRNLDPFMMGEAGCIDTFTRATQGADIAVIEGVMGLYDGIDGTD
                     CASTAHVARILDAPVILVIDIKGMSRSALALIRGYQDFDTTISFAGIIFNRVGSQRHR
                     QMIEGEIPLPSFGWIPKREDIAIESRHLGLKMAYETGPMEKFGSLIEEFCDLDAVLAS
                     ARDHPGSKPQKKSPITVKKKRVRATIGVAFDEAFCFYYQDNLDRLRAWGAELVFFSPL
                     TDRLPYTDALYFGGGYPELHLPALESSPCTREVKTVADAGMPVYGECGGLLYLTREIR
                     SDRAYRLCGVLPAESEMTARIQALGYVKGKSTGDMAFVPALQGIQGHEFHYSRVLPEH
                     DAQFAFRLTRGKGIDDGRDGLISHNTLGTYTHAYFSNAMVKKFIDAAVCFSHE"
     gene            complement(7374..8576)
                     /locus_tag="VFC43_01705"
     CDS             complement(7374..8576)
                     /locus_tag="VFC43_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZK29937.1"
                     /translation="MSSIILEARDVWYRYPRGKEAISGISFHIRKKEKIALVGPNGAG
                     KSTLLKMFNGMIRPDSGLMLFDNQPIGYDRASLRTLRKRVGFVLQNPDRQIIAPTVYQ
                     DVAFGPVNLGYSEHDVKRVVTQALSHVGLEGFERRPPHQLSGGEKKRVAIAGVLAMDP
                     DVLVFDEPTSGLDPSGSEDIMELLDELNHEGKTIIISTHNIELAYPWADRAMLMLEGT
                     ILKEDIPEKAFGNPEFVRRAHLSLPTLLELHGELLNRGFPLIGKKPHTVLDMMHCIES
                     MYEKIARPSTPGLITVCNVDRDDREQLVAWLASRPGISVGAMGTRAKQQAEISRIRLD
                     FTYGVIDKCILKALLGENSLIMTTDSMVLRVSQRVAAYCQESKIRLTVTMFTPSEGPG
                     DTGNGKIP"
     gene            complement(8587..9375)
                     /gene="cbiQ"
                     /locus_tag="VFC43_01710"
     CDS             complement(8587..9375)
                     /gene="cbiQ"
                     /locus_tag="VFC43_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HZK29938.1"
                     /translation="MFEELLEDIAQKNGIREVNTMLKLGAGLGAIILCLLSASYVPPL
                     FIALILTAAILFLARVDAKTYAELFIVPLWFAIMSVAGIILISGGQDIFWKWDILSSF
                     SLSVTRESINQGVFVFCRVLGGMSAMCFISLTTPMTDIFTILRRCRVPETVIDLMMII
                     YRTIFILMDQVIQIYQAQVMRLGYSTWRESVHSFSSLCGAAFIASWNSGEDLIRAMDA
                     RCYEGKFALMGENRPVERLPLLALVVFLSITAAVVVVAGNRTII"
     gene            complement(9406..9708)
                     /locus_tag="VFC43_01715"
     CDS             complement(9406..9708)
                     /locus_tag="VFC43_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HZK29939.1"
                     /translation="MKFRYTLELLTVTALIAFCLVFLYTSSAMKGAGFAGSDNEGSGL
                     IAELSGTPQENFTPLIPQWHPPGGEIESCLFALQAAIGGLFVGGVFGFWIGAQKKP"
     gene            complement(9705..10400)
                     /locus_tag="VFC43_01720"
     CDS             complement(9705..10400)
                     /locus_tag="VFC43_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HZK29940.1"
                     /translation="MHIMEGFLPWQWCLVWWIVALPFILFGIYQLKKILAADRETLPL
                     LGVTGGFIFILSALKLPSVTGSSSHPTGTGLSTISFGPWITSVVCAIVLLFQSLFLAH
                     GGLSVLGANIVSMGIAGPVVGYGMYRLLRDTSVNIYITVFFVTALADIFTYVTTSLEL
                     ALAYPAETGGFVSSFILFMGIFAVTQLPLAIIEGIVLALVFKYIVALKPDILVRLKIF
                     SEEKIDNARNDAS"
     gene            complement(10563..11867)
                     /locus_tag="VFC43_01725"
     CDS             complement(10563..11867)
                     /locus_tag="VFC43_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HZK29941.1"
                     /translation="MRFSEKTVYILLVLCTCSIAPALAGTEYMSGSPQIFASVAGTNE
                     YSPGNDVQITITIQNTGLNEFKFVQRGIIDREDLPNTAKSVTVGLNAGTAPVVIKSDP
                     QMLGDIKGTASANAVFTTKINTDAAAGRYTLPLFVNYTYLYKAEQFGGDTIQYTYKQA
                     NETLGIPITIQSDISIDVLSATPGHLNVGTEGYLDLKIKNTGSEDGIKAIVKILRNGN
                     SPIMPTDSSVYIGDFRSGSTVECRYKVSVSGDAGRQTYPVDVVVVYQNSDGEFITSRT
                     DTVGIPIGGKVDFAVISPPAEMNPGNKKVITVEYKNLGETAIYSAQSRISAVDPFTSN
                     DDIAYLGDLLPGKTAVASYEVSVDRSATIKQYALDSEIRYRDALDTTYLSDTMKVKIS
                     VNSPSGITAVVSNPVYLALIVAGVIGLAYGVFHFRKKNNNLR"
     gene            12107..12730
                     /locus_tag="VFC43_01730"
     CDS             12107..12730
                     /locus_tag="VFC43_01730"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HZK29942.1"
                     /translation="MYIEHICFNLALAILVTLLLADQKNAGWYTAVILVSGCVPDIDG
                     IFSLIQSPPVFTGGIIPGMAFHLRYFHSIGILCIYAVLVAGVLAYYHRLDFRIIAIFA
                     GTGFGAHLLEDAIVYNPASAVFWPVSSQEVGIGIFPLYSRDFLSIANAEVFGIGILLL
                     VLMVSSSILLKKTGWASLPPVLTPIPPAVFSAVPRILLVFRSLIRSR"
     gene            complement(12737..13396)
                     /locus_tag="VFC43_01735"
     CDS             complement(12737..13396)
                     /locus_tag="VFC43_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29943.1"
                     /translation="MKEMTAGDDNSRYLYDDLLEWQYGNTAYYSALVILLFIAVDGLI
                     IRGDVLRAQWGFVALAIALLPALAEYLTPLILPWLMKLFITLSLIFHIAGGIFGFYFA
                     LYPVYDKIGHLVSSIAISLVIVVILLILVGITGIRLSSPAVVICIFIPVMVCGVAWEY
                     AELNIDTVAGSTYFVNINDSILDMLFNVMGTCYIVLNIRELLKRESLMDLYRRSIHWK
                     S"
     gene            complement(13503..15203)
                     /locus_tag="VFC43_01740"
     CDS             complement(13503..15203)
                     /locus_tag="VFC43_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZK29944.1"
                     /translation="MVEGSYASGTSSFPLLGMTIGKMVNSIAEKYPDNEAVVSVHQDI
                     RWTYREFLERVNQVARGLMGLGVEKGDRVGIWAMNHAEWVVVQFATAKIGAIMVNINP
                     AYRTYELEYALKQSEIRTLIIQGRFKTSDYVGMFYEACPEAYEAKPGRISSDKFPFLK
                     NAVFLGDIPYNGMFTWNELLKKAEEVTNDELIERGEGLAFDDAINIQYTSGTTGFPKG
                     VVLTHHGVLNNGYIIGEGMGFTEKDRLCIPVPFYHCFGMVLSNLACVTHGSTMVLPAP
                     TFDAEEVLKTVEKERCTALHGVPTMFIAELTHPSFEKYNLKSLRTGIMAGSPCPIEVM
                     KQVNTKMHMSEIVIVYGQTETSPGVTMTTTKDPVERRVSTVGRAFPHTELKIIDSKTG
                     RIVPFGEIGEICARGYCVMKCYYNNPSATHATLDSNNWNHTGDLGTMDEEGYFRIVGR
                     LKDMVIRGGENIYPREIEEFLHHHEKIADVYVVGVPDKKYGEELCAWVKVKEGQKLTE
                     EEVRDFCKGKIAHFKIPRYVMFVDDFPMGVTGKIQKFRMREDSIKSLGLEAAEKIETA
                     "
BASE COUNT         3754 a         3797 c         3719 g         3965 t
ORIGIN      
        1 gactgcatag gcagctccac caagaataac cagcgggagt aacgtgaaga agatgatgat
       61 cagtgcatcg atcatgccaa tgaaccctgc gatgccctca ttgatggttg atacgaagtt
      121 atgtccggtc tctccgccaa cgggttcagg ttcctcgagg ttcacggtga ttgtggagag
      181 atcgatccgg ctgttgagat atttcagcct gccttcaaga cggttcagtt ctgtctggac
      241 gcggtcgatc tgctgctgga ctgttatgac gtcttccacc ttgactgctc gtttcatgat
      301 ctcattatat tgcgccagct ggttctggta tgaggttttc tgagcctgga tatcgatata
      361 ctcctcggtt acatcctgtc cctgggttga agcggatttg accgtgccga gtgccctgac
      421 acctgcaagt gtggcatcaa attcgggtgc gggaacgcgg agagttacgg aaccggaaag
      481 acggtcgtta taatttttct ggatatttgt cgaagaaata taaccccctt tctgggcagc
      541 cagagttctt aagatctcga ctgcgccagg cacatctttt acctcgatgg ttaagtatgc
      601 tgtttttatg atctttgtat caattccggt tgtaccgggt cctgactggg taggggccat
      661 tgttggaaca ggcatggaag ccggaccaga atatgccgtt ttttcagcaa gactacctcc
      721 attgctggca tcctgggata ttgaggatcg accggtactc gtgctttgca accccgtgca
      781 cccggcagct gcaaccattg ctacgcacac cacaaggagt gcgatgattt tataattcat
      841 atacaatgca tcgccgtggg agatatttat atccgtcgta aacttcgaat tgttttgcag
      901 ttatcatacc agcaacccgg tttagccagg atgtattcat ttcaacgaac atccggttta
      961 ttggcagatg cgtgatcgct cttatataac aatacacgtg atattttcat ggaattatta
     1021 tggctgaata tagtatacct gaaaaaccgg aaggggcagc ggaactcgag aaaaagggtg
     1081 ttgaagacct cgtgcatgcc cttcgcgaca gcacggatcc catggtccgg caatattccg
     1141 cataccttct cgggaaggct cagaaccccc gtgcaatcca gccacttgtt gaagcactgg
     1201 gagactttga caagtccgtc cgcgaacagg caactcttgc cctgtcagca atcgggaaag
     1261 cagctattgc accattgtct gctgccatga aggaacctaa gtgggagacc cgctaccgtg
     1321 cggctgaagc cctcgggaag attgctgatg aaaaagctgt ccagcctctc atccaggccc
     1381 tgaaggataa ccgggaccat gtacgctaca tggctgcaaa aggcctgcgt gaattcggca
     1441 attctgaggc ggttgacccg atgattatcc tattgaagga tgaaaacagg tatgtccgga
     1501 tgatggccat ccgggcactc ggagccatcg gtggaaaaaa agtgcacgat gccctgaaac
     1561 aggcgtttga gtcggaatct gatgagaaag ttaaggaagc gatcactgaa gcaatgaaat
     1621 agaaatatta tccttttttt tgttttattc atgtcccttg ataatgaggg attattgata
     1681 tctgatgcat aacgccgccg tttgtaatgc tcattgcacc atgaaagact caaggaatat
     1741 atactcagcg gaaaagcagt atttgtttcg gggagattaa tcgctgcgat taatattaat
     1801 tgtcgtgatt aatatgtgac gaaatttact attgttactc tattggatct tctgtggtgg
     1861 gtatgaccga aaaaaagcct tttagtttaa aaaaccggtc attctttgtt tctatccttt
     1921 ttgcaatgat actcctgata ttgattgtcg gagccctcgt tgtagtaaat gattactaca
     1981 atacaaaaaa tatctttgac aaaaactccc agcacctcag gcaaatgacg gaacaggata
     2041 tcatcgtcat tatcaaactc actgatgaaa gctacaacct gtatgacagc agcctgaatg
     2101 agcagatgcg caggggattt gacagtgtac tggaatcata taaacagtcc tcgggaaatc
     2161 cttccctcat gaaccttact gatcttaaaa tgaatcttgg agaagatttt gatatctata
     2221 ttatcaatga atccggggtc attgagttca ccacctatgt acctgaacgg ggaatggact
     2281 ttaaaaaaat cccgtatttt tttgcctacc tgacaaagat ccggaattca gaagggttct
     2341 ttcccgatcg tattgtcgag gaccagatgg gtaccgggaa aacccgcaaa tttgcctata
     2401 tgcccactcc cgaccaccgg tatgtactcg aacttggata ttcaagaact tcattttcgg
     2461 gtgagcgggc ttccatacga tataaaaacg caattgaccg gatcgcctca tttaatccct
     2521 atatcgaacg cgtccgtatc ttcaactcga tgggaaaaat tgctgacaat acctctgaac
     2581 cagtggatga acccacacgg cttcttttag cggagttgat gcaggagcgg caggatcata
     2641 cggtcaaact ccccgaggcc ggaaagtccg taaaatacct cttcattgac ctgaaaaatg
     2701 aactgtatgg ctccgacgcg agccgtattg tcgagatcac atatgatgat gcgatgcttg
     2761 aaagagcatt tttggagcat gtaaagttcc ttctggtcgt cttcatcatg gcactcacag
     2821 taggaatctg tgctgctttt gtgctgtcac gatatctcat aaaaccgatc tcgggaattg
     2881 taaaggatgt caatcggatt tctgacggtg aacttgacct gaagatcgct tcaacccacg
     2941 ttacagaatt tcagaacctg gaacagagca tcaatacgat ggtatcgtcc ctgaaaaagg
     3001 catttcgtga agtccaggat gaaaaatcgt tccagctgga gatggttgac cagctgcctg
     3061 ttgctgtttt tatgaaaggt gtacaggatg gaaaatacgt attctggaat aaagcgagtg
     3121 aagagatttt tgacctcact tcatctgaag tgattggcag aaccgacaag gacctgtttt
     3181 cagaacatat ggtatctgtc attgaacagg aagatgagga agcgtgtctc aaacgtgtaa
     3241 tgatattgaa taaaaaaatt gtccacaaat cccgcggcca gcgaattatc catatgattc
     3301 ttgtgccgat tttcgattca gccaaaaccg tccggtacat tctcgggatt ggagaggatt
     3361 taaccggcga gaccctcaac atgaaaaccg atctcctgtt cagtatcaca cggcgtgata
     3421 ttttggatca gttgtcagat atcgtcagtt acctggagcg ggcgcagctg aaaatctctc
     3481 acgaggcaac acagacattt tttgataaga cccttgagtc ggttgagtct atccggaacc
     3541 atatggcatt tgtaagttcg cttcaggcaa tgggaataac aacgccaaca tggcaatcgg
     3601 tgatgaagtc attctggagc gctgttgcct tgttgccggc caaaaaaatt gatattcgcg
     3661 ctgatatgga tgattttgaa ctctttgcag atcccctcct ttcccgggtt ttttacaatc
     3721 ttttagtaaa ctcgctgcaa cacgggaacc cgcagatgac aaaaatccgg ttccaagcca
     3781 ggcaggtggg agactctctc atcctgtcat atgaggataa tggcatggga attcccgctg
     3841 atgaaaaaga gaaaatattt gaatacggct atggtaagag gaccggttta ggtctgttcc
     3901 ttgcccgtga gcttcttggc tataccggca tgacaataac cgaaacagga gtgcccagaa
     3961 tgggtgcgag atttgagatc gttgttccga agggcaaatt caggaaagta acttcagaat
     4021 aacgggctca atccacgatc aggtgactaa attggcgcga ttggagcatg gattattttt
     4081 aaaaaaacac acatagtcca ctgttaaaaa tcgcaaaaat gataggggta acgcttcgct
     4141 acaagtggat cgcctcagcg atgtgcatgg ttcccggtct ggagcccgtc ctcccatcag
     4201 ggaggctatc acaaaaaagt aaacatatca cctggttctg gctccctggt aggtgataaa
     4261 aacacacaat ccgttttgta tttttaaaaa aaagttttct cgagagaaaa tcctgtttat
     4321 ttctggaccg ccccgaggtt tgcctgcccg acggtttttg cataccgggc cagcacaccg
     4381 gacagggatt tttgcagggg ttttaaagtc tttttacgtt tcgacagtgt cgtttcatcg
     4441 accagcaggt caatcttttt taagaaaagg tcgatctcga tgcggtcgtt atccttcacc
     4501 agggctatgg ggccacctat tgccgcttcc gggctgacat gccctatgca gggaccccgc
     4561 gtgcctccgg agaacctgcc atcggtgatc aggacaacac gtgtataccc aaatcccatg
     4621 agtgcggaag tgggtgagag catttcgggc ataccggggg ccccacgggg accttcatac
     4681 cgtatgataa tgacatcgcc ttctttgatc tcacgggaaa ggatcgcttt cattgcctgc
     4741 tgttcgccat caaagacccg tgccggcccg gcatgtttcc acattttctt tggaaccgcg
     4801 gcgcatttca ctacggcacc atccggagcc agagatccaa aaagaatttt cagccctcct
     4861 gcaggactga tgggatcccg tgttgaccgg attacttctt catttttcac atcggcactt
     4921 ctggcaatct gcaggatttt attaccggac accgtcgggc tgtcttcaag gtacttttcc
     4981 agccttttca atactgcggg aatcccgccg gcccgataga gtgtctgcat cgaatgcggt
     5041 ccggaaggct gcatatggca aatatgggga acaccatctg caagtgtatt aaagtcctct
     5101 aaggagagcg ggacgtctgc ttcgaccgca attgccatca ggtgcaggac ggtatttgtc
     5161 gatcctccca gtgccatatc cacccggatc gcattcctga ggctcttttt tgtaataata
     5221 tcgcggggcc tgacctgttt ttttacgagg gggatgatcg cttctccggt ctcccgtgca
     5281 attctgagtt ttcctgcatc gacggcagga attgcagcac agccggaaag cgacattccc
     5341 attgcttctg tcatgcatgc catggtattt gccgtgtaga ggccctgaca gctcccgcag
     5401 cccggcattg cacaagcctc cagttcgcat aattcgggtt cgctcatcgt ccctgccgcg
     5461 acttttccca caccctcaaa aatatcgata agggacaggt cccttccgtc ctggtacccg
     5521 gacaacatcg ggcctccggt aaggataact gagggtatat tgcatcgggc agctgccata
     5581 agcatacccg ggactatctt atcacaggtg ccaatgcaga caagcccgtc gaaacgatgc
     5641 gcctgaacca taagctcgac ggaatcagcg atattctccc gtgatggcaa cgaataccgc
     5701 attccctcgt gacccatggc aatgccatcg caaatgccaa tcacgccgaa ttcgaagggg
     5761 acgccccctc cggcagcgat gccctcacgc accttctccg caagctggcg aaggtgaaca
     5821 tgcccgggaa caatattatt gtacgcattg gcaattccaa tgaacgggag tcccatctcc
     5881 tggtcggtca ccccgagtga ccggaggagc gaacggttcg gggctcgctg atatccggat
     5941 tttatttcat cgctgcgcat ctctgctcac cagtgttctg tgagatattc gccccgcaac
     6001 aattttatgc cttctccttg aacatcaacg ggaaattttt tactcgtgag aaaaacagac
     6061 tgcggcatcg atgaactttt ttaccattgc gttggaaaaa tacgcgtgcg tatacgtacc
     6121 aagcgtatta tgggagataa gtccgtctct cccgtcatca attccttttc cccgtgtcag
     6181 gcggaatgca aactgtgcat catgctccgg aagcacacgg gagtaatgaa attcatggcc
     6241 ttgtatgccc tgcaatgccg ggacaaatgc catgtccccg gtgctttttc cctttacata
     6301 ccccagcgcc tggattcttg ctgtcatctc tgattcagcc ggcagcacac cgcataaccg
     6361 gtatgcccgg tcagacctga tttcacgcgt gagatacaaa agccccccgc actcgccgta
     6421 taccggcatg ccggcatcag caactgtttt tacttccctt gtgcacgggg aagattccag
     6481 ggcaggaagg tgaagttcag ggtaacctcc tccaaaatag agggcatcag tatacggtaa
     6541 cctgtcggtt aagggactga aaaagaccag ttcggcaccc catgcccgta agcggtcgag
     6601 gttatcctga taataaaaac agaatgcctc gtcgaatgcg acgccgatgg tggcgcgaac
     6661 ccttttcttt tttaccgtta ttggactctt tttttggggt tttgaacccg gatgatcccg
     6721 tgcggatgcc aaaactgcat ccaaatcgca gaactcctcg atcagggaac cgaatttttc
     6781 cattggtccg gtttcatatg ccatcttaag accaagatgg cgactttcaa tcgcaatatc
     6841 ttcgcgttta ggtatccagc cgaatgatgg caacgggatt tctccctcaa tcatctgccg
     6901 gtgtcgctgg cttcccacgc ggttgaagat gatcccggca aacgagattg ttgtatcaaa
     6961 gtcctggtat ccccgtatga gtgccagggc actgcgggac atccctttaa tatcgataac
     7021 caggatgacg ggtgcatcca ggatgcgggc gacatgggcg gtactggcac aatccgtccc
     7081 gtcaatcccg tcatagagtc ccatgacccc ttcgatgact gcgatgtcgg ctccctgagt
     7141 tgcccgggta aaggtatcaa tacagccagc ttctcccatc ataaagggat cgaggttccg
     7201 gcaaggacgg tgacagatcc tggtatgata ggaagggtcg ataaaatccg gtcccgcttt
     7261 gaatggctgg actttaagcc cccgtgcagt gagcgccgcc atgatgccgc tggcaatggt
     7321 ggtctttccg cacccgctgt gggtgccggc tataacgatc cggggtactc cgttcatggt
     7381 attttcccgt tccctgtgtc cccaggtccc tctgacggcg tgaacatagt caccgtaaga
     7441 cggattttac tttcctggca gtacgctgca acacgctggc tgacacgcag gaccatgctg
     7501 tccgtggtca tgatgagcga gttttctccc aacagggcct taagaatgca cttgtctatt
     7561 acaccatagg taaaatcaag acgtatcctg gaaatttcag cctgctgctt tgccctggtt
     7621 cccattgcgc ccacagaaat acccggccgg gaagctaacc atgccacgag ctgttcccgg
     7681 tcatcccgat caacattgca taccgttatc agcccgggcg tggaggggcg ggcgattttt
     7741 tcatacatac tctcgatgca gtgcatcatg tccagcaccg tatggggttt tttcccgatg
     7801 agcgggaatc ccctgttcag gagctccccg tgtaattcaa gaagcgtcgg gagagacagg
     7861 tgtgccctac gcacaaattc gggattacca aatgcttttt cgggaatatc ctctttgagg
     7921 atcgttcctt ccagcatgag cattgcgcga tcagcccacg ggtaggccag ttcgatatta
     7981 tgcgtggaga tgatgatggt cttaccttca tggttcagtt catcgagtaa ttccataata
     8041 tcttcagatc cggatggatc aagcccgctt gtaggctcat caaagaccag tacatccgga
     8101 tccatggcaa ggacaccggc tattgcaacc cgcttttttt cacccccgga gagctggtgg
     8161 ggagggcggc gttcaaaacc ttccagcccc acatgggaaa gtgcctgcgt tacgactctt
     8221 tttacgtcat gttcagaata tccaaggttc accggtccga atgccacatc ctggtacacc
     8281 gtcggtgcaa taatctggcg gtccgggttc tggagaacaa aacccacgcg tttgcgcaat
     8341 gtgcgcaggg atgccctgtc atacccgatt ggctggttgt caaagagcat gagcccggag
     8401 tcagggcgta tcataccgtt gaacatcttc aggagggtgg attttcctgc cccgtttggc
     8461 ccgaccagag caatcttttc cttcttccgt atatggaagc tgatgccact gatggcttct
     8521 ttgccccggg ggtaacgata ccacacatcc cgtgcttcaa gtataatgct gctcatagtc
     8581 tgcgacctag attatcgtcc tgttgccggc tacaaccaca acagcggctg tgatggacag
     8641 gaacaccacc agcgcgagga gcggcaatcg ttcaacaggg cggttttctc ccatcagggc
     8701 aaattttcct tcataacatc gtgcgtccat ggcgcggatt aaatcttccc cggaattcca
     8761 gctggcaata aatgctgcac cgcacaggga tgaaaacgag tgcactgact cacgccacgt
     8821 gctatatcca aggcgcatca cctgtgcctg gtagatctgg atgacctggt ccatcaggat
     8881 gaagatggtg cggtagataa tcatcatcag gtctatgaca gtttcgggga ccctgcacct
     8941 ccgcaatatg gtgaaaatat cggtcatggg cgttgtgagg gaaataaagc acatggccga
     9001 catccccccg agcacccggc agaagacaaa aacaccctgg tttatgcttt ccctggtgac
     9061 agacagggaa aacgatgaaa gaatatccca tttccagaat atatcctgtc ctccggaaat
     9121 gaggatgatg cctgcaacac tcatgatggc gaaccagaga ggtacaataa aaagttcagc
     9181 ataggttttt gcatccaccc gggcaaggaa caggatcgca gcggtgagaa taagggcaat
     9241 aaaaagggga ggaacgtaac tggcagagag taggcagaga atgattgcgc caagacccgc
     9301 gccaagtttg agcatcgtat tcacttcccg gatcccgttc ttctgggcaa tatcttccaa
     9361 aagttcctca aacacagcaa tgcactcctc tttactataa gacgattatg gtttcttctg
     9421 tgcacctatc cagaacccga atactccccc gacaaataat cctccgattg cagcctggag
     9481 cgcaaacagg caggactcga tttccccgcc tggtgggtgc cactggggta tgagaggggt
     9541 aaaattttcc tgcggtgtac cggatagttc tgcaatgagt cctgaacctt cattgtccga
     9601 accggcaaat cctgctcctt tcatggccga acttgtgtac aggaatacta agcagaatgc
     9661 tataagggcg gtgaccgtaa gaagttcaag ggtatacctg aatttcatga cgcatcgttc
     9721 cgtgcgttat cgatcttttc ttcggagaat attttcaggc ggaccaggat atccggcttg
     9781 agagcgacaa tatatttgaa tacgagtgca aggacgatgc cttctattat agcaaggggg
     9841 agctgggtga ctgcaaaaat acccatgaac aggataaaag agctgacaaa tcctccggtc
     9901 tctgcagggt atgcaagggc cagttcaagg gatgttgtca cataggtaaa tatatcggca
     9961 agggcagtaa caaaaaagac ggttatgtag atatttaccg acgtgtcccg gagcagccgg
    10021 tacattccat aaccaacaac aggtcctgca atgcccatcg agacgatatt tgcaccgaga
    10081 accgaaagtc ctccatgtgc gaggaacagg ctctggaaaa ggagcacgat tgcacatact
    10141 accgatgtga tccaggggcc aaatgatatt gtcgagagtc ctgtgccggt aggatgggaa
    10201 gaactgccgg ttaccgaagg gagttttaag gctgacagga taaaaataaa acctccggta
    10261 actcccagga gcggaagggt ttcacggtct gcggcaagta tttttttcag ctggtaaatt
    10321 ccaaacagga taaagggcag ggctacgatc caccagacca gacaccattg ccagggcaag
    10381 aaaccttcca tgatgtgcat gttatctcct gttcgtatcg gtttttgcgt actaattaaa
    10441 ttttacttga tttaattcca ttttaattgc atagtatgtt gcatacgcca atattaaata
    10501 tttcttgttt ttttaaaaaa ttccattttt aaaaaaaatc tgatactgat cagtcatcgg
    10561 gtttatcgca ggttattgtt ctttttccgg aaatgaaaca ccccgtatgc caacccgatt
    10621 actcctgcga caataagggc aaggtagacg ggatttgaga caaccgcagt tattcctgaa
    10681 ggtgaattca cgctgatttt tactttcatc gtgtcagaca aatacgtggt gtcaagggca
    10741 tcacggtacc tgatctccga atcaagtgca tactgtttga tggttgctga acggtcgaca
    10801 cttacctcgt atgatgcaac cgcagtctta ccgggcagga gatctcccag gtacgcgata
    10861 tcatcgttac tggtgaacgg gtcaacggcg ctgatcctgc tctgggcact gtagatggca
    10921 gtttctccca ggtttttgta ttccaccgtg atgacttttt tattcccggg gttcatttct
    10981 gccggggggg agatcactgc aaaatccact tttcccccga tgggtattcc cacggtatcg
    11041 gtccgcgaag tgataaattc cccgtcgctg ttctggtaca ccacgacaac atcaaccggg
    11101 taggtctgtc gcccggcatc accggatact gaaaccttat acctgcattc aacggtgctg
    11161 cccgatctaa aatccccgat atacacacta ctgtccgtcg gcattatggg actgttgccg
    11221 ttccgaagaa tttttacaat agcttttata ccgtcttctg aaccggtatt cttaattttc
    11281 aggtccaggt aaccttcagt gccaacgttc aggtgcccgg gtgttgccga tagtacatca
    11341 atcgatatgt cggattgtat ggtaatcggg atcccgaggg tctcatttgc ctgtttatag
    11401 gtatactgga tggtatcccc gccgaattgt tctgccttgt acaggtacgt ataattgaca
    11461 aaaaggggga gtgtatacct gccggctgcg gcatcggtgt tgattttggt ggtaaacacc
    11521 gcatttgcac tggccgtacc tttaatgtcg cccagcatct ggggatcgga tttgatcacg
    11581 acgggtgcag tgccggcatt gaggcccacg gtgacagact ttgccgtgtt ggggagatct
    11641 tcgcggtcaa tgatcccgcg ctggacgaat ttgaattcat tgagcccggt attctgaatg
    11701 gtgatggtga tctggacatc attgcccgga ctatactcgt ttgtgcccgc gacagaggca
    11761 aaaatctgcg ggctccccga catgtattca gtcccggcca gtgcaggagc aatcgaacat
    11821 gtacaaagaa caagtaaaat gtataccgtt ttttcactga acctcatcag gcagctccgg
    11881 gtagagattt cttagtatca ttgttctcat ctgttggtga taagatatcc ttcatggcat
    11941 gatcttactc cggattaatg attgtcaggt ttgccagaac actttttgga aatttcatcg
    12001 ccggcatagt tcttttacac ggaataattt cggattggca ttatccattt tctctctctt
    12061 gtcagcacca gtacctattt gactcagttg tacaaacgtt ggtataatgt atattgagca
    12121 tatctgcttc aatctcgctc ttgcgatact tgtaaccctc ctgctggcag accagaaaaa
    12181 tgcaggctgg tataccgccg taatcctggt cagcggctgt gtgcccgata ttgatggtat
    12241 tttttccctg atccaaagcc cgccggtctt taccggaggg atcatccccg gcatggcatt
    12301 tcatctccgg tatttccaca gcatcgggat tctctgcata tacgcggtac tggttgccgg
    12361 agtgctcgcg tattatcacc gcctggattt ccggataatc gcgatctttg ccggaacagg
    12421 gtttggtgcc cacctcctcg aggatgctat agtgtataat ccagcttctg cagtattctg
    12481 gcccgtatct tcccaggaag tcgggatagg aatctttcct ttgtattcga gagatttcct
    12541 gagtatagca aatgccgagg ttttcggtat agggatactt cttctcgtgc tcatggtgag
    12601 ttcaagtatc cttttaaaaa agacgggatg ggccagtctc ccccccgttc tcaccccgat
    12661 tcctccggca gttttttcag ctgttccgag aattcttctg gttttccgct ccctcatccg
    12721 gagtcgctga cgatatttag gatttccagt ggatggacct gcggtacaga tccatgagag
    12781 attcgcgttt cagcagctca cggatgttga ggacaatata acatgtgccc ataacattga
    12841 atagcatatc cagaatagaa tcattgatat tcacgaaata ggttgatccg gcaacggtat
    12901 cgatattcag ttctgcatat tcccatgcta ctccgcacac catcaccggt atgaatatgc
    12961 agatgacaac tgcaggacta ctcagccgga tgccggttat acccacgagg atcaggagta
    13021 tgaccactat cacaagagag attgcgatcg atgataccag gtgcccaatc ttgtcatata
    13081 ccgggtacag ggcaaaatag aacccgaata tccctcctgc gatatgaaag atgagcgata
    13141 gcgtgatgaa caacttcatt agccagggaa gaatgagcgg tgtgaggtat tcagccagtg
    13201 cgggaagcag tgcaatcgcg agtgcgacaa aaccccattg ggcccggagc acatcgcccc
    13261 gtatgatcaa cccgtctact gcgatgaaca ggaggatgac caatgctgaa taatacgcgg
    13321 tattcccgta ctgccattcc agcagatcat cgtagagata ccgggaattg tcatcacctg
    13381 ctgtcatttc tttcatacga attgcctgaa acggtaaccg cccggctttt tttggtttcg
    13441 gaggtgttgg gtttcaggag cagtatccat cacaatatcc ctgcaaatat ccccggatca
    13501 tctcatgcgg tctcaatctt ttcagctgct tccagcccga gcgatttgat cgagtcttcc
    13561 cgcatcctga atttctgtat cttgccggtg acacccatcg ggaaatcgtc aacaaacatc
    13621 acatagcggg ggatcttgaa atgggcaatt ttacctttac agaaatccct gacttcctct
    13681 tctgtcagtt tctgcccttc tttgactttg acccatgcgc agagttcctc gccgtatttt
    13741 ttatccggca ccccgactac gtagacgtct gctatcttct catggtggtg taagaactcc
    13801 tctatctcac gcgggtagat attctctcct ccccggataa ccatatcctt gaggcgaccc
    13861 acgatcctga agtacccttc ttcgtccatg gtcccgaggt ctccggtgtg gttccagttg
    13921 tttgagtcaa gtgttgcatg agttgcagac gggttattgt aataacattt catcacacaa
    13981 taaccccgtg cgcagatctc cccgatctca ccgaagggga cgatccttcc ggtcttggaa
    14041 tcgatgatct taagttcagt atgcgggaac gcacgcccga ccgttgacac acgccgctca
    14101 acaggatctt ttgtcgtggt catcgtgacg cccggtgagg tttctgtctg gccgtatacg
    14161 atcacgatct cgctcatgtg catctttgtg ttgacctgct tcatcacttc aatcgggcag
    14221 ggggacccgg ccatgatgcc ggtccgtaac gactttaagt tgtatttttc aaaactgggg
    14281 tgcgtcagct cagcgataaa catcgttggt accccgtgaa gggcagtgca ccgctctttt
    14341 tccacggttt ttagaacctc ttcggcatca aaagtgggag caggcaggac catggttgat
    14401 ccatgggtca cgcacgcgag gtttgagagg accatgccaa agcagtgata gaaaggcacg
    14461 gggatacaga gacggtcttt ttcggtaaag cccatacctt cgccaatgat atagccgtta
    14521 ttcaggactc catggtgggt aagcacaact cctttcggga acccggtcgt gccacttgta
    14581 tactggatgt tgattgcgtc atcgaaggcg agcccctcac cccgctcgat cagctcgtcg
    14641 ttggtcactt cttcggcttt tttcaacagc tcattccagg tgaacatacc gttataggga
    14701 atatcgccaa ggaagaccgc attttttaag aacgggaact tgtctgatga gatcctgccg
    14761 ggctttgctt catacgcttc ggggcaggct tcatagaaca tcccgacgta atctgaggtc
    14821 ttgaaccgtc cctggataat gagagtacgg atctcggatt gtttgagtgc atattcaagt
    14881 tcgtaggtac ggtatgcggg attgatgttc accattatgg cgccgatttt tgccgtcgcg
    14941 aactggacaa caacccattc agcatggttc atggcccaga taccgacccg gtctcccttt
    15001 tccacgccaa gccccatcag cccccgggcg acctggttta cccgttcgag aaactcgcgg
    15061 taggtccagc ggatatcctg gtgcaccgag acaaccgctt cattatccgg gtatttttcc
    15121 gcaatactgt tcaccatctt gccaatcgtc attcccaaga gaggaaacga cgaagtcccg
    15181 ctcgcgtagc tgccctcgac catccttaaa atatggggtg agaacaggat atatg
//
LOCUS       DASRLV010000044         7862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_178941,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000044 DASRLV010000000
VERSION     DASRLV010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 7862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7862
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178941"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VFC43_01745"
     CDS             complement(<1..475)
                     /locus_tag="VFC43_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29945.1"
                     /translation="MMDKKWIIFLLVLSLTGIIVLTGCVSQQPTPNPAATPDLTNQKS
                     PETATFTTYPALTRLHTPDVPAGLRFVAGGFTAPLDIADPHDGSGRLFAVDQTGYVKI
                     FFMNGTVVEQPFLDVRDRVVKIDPTYDERGLLSLAFHPQFATNGRVFVYYSAPLRA"
     gene            771..1610
                     /locus_tag="VFC43_01750"
     CDS             771..1610
                     /locus_tag="VFC43_01750"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29946.1"
                     /translation="MKKTALSFILVVLIATVFITGCTQQPAQPTPQPTTVQPADTIKV
                     SDSVLGRILVDAQGKTLYYFANDIPSRANSSCYGQCAIIWPVFSSDITVSPPLVSSDF
                     ASFTRADGLKQTAYRGWPLYYYQQDPKPGDLNGENVLKVWFVVKPDETVMIAYQGTVG
                     SFLTDKTGKTLYTFSQDTPGTSACTGACLTKWPAFNASSINAPSVLKPANFSAVTRAD
                     GVLQTAYMNRPLYYFADDAKPGDVKGQGFNNVWYVANVTGFVPVPTTVPTTLRTLSYG
                     GGY"
     gene            complement(1741..1947)
                     /locus_tag="VFC43_01755"
     CDS             complement(1741..1947)
                     /locus_tag="VFC43_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29947.1"
                     /translation="MKIEKTGENAQRHKELIIRMSEILDAHGSGQYIPASELIDLIAN
                     EPTFTKQDCVYLGLYLAANNTDKK"
     gene            complement(2065..2412)
                     /locus_tag="VFC43_01760"
     CDS             complement(2065..2412)
                     /locus_tag="VFC43_01760"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HZK29948.1"
                     /translation="MQVTFFTPLTSLITGRTVDPKLAMTGKITLSGTVLPVGGIKEKI
                     LAAHRAGIKKVILPKENAWGLEEVPEEVRGELTFVIVETIEEVLKEALDIDLPHGPVA
                     ETPENRCVPAQNL"
     gene            2566..3126
                     /locus_tag="VFC43_01765"
     CDS             2566..3126
                     /locus_tag="VFC43_01765"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HZK29949.1"
                     /translation="MDKIKLGPMPYMSVMPTLLVGANVRGKPNYMTAAWATVACMAPP
                     MVCVAINKARYTAKGIEENKTFSLNVPSASQVVETDHCGLVSGSLEDKSGVFESFYGS
                     LKTAPMAKECPVSIECTVFKSVDCGSHLLHIGQIVEIYADKTCMTDGKPDITKINPIV
                     YAQATYFDIGKQVDRAFFAGKAYRKE"
     gene            complement(3403..3705)
                     /locus_tag="VFC43_01770"
     CDS             complement(3403..3705)
                     /locus_tag="VFC43_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29950.1"
                     /translation="MSGTTYTIKQFGVISVGKFFAVFGLVWGFFVGIFLAAGLGGMGS
                     VLGSHGLGIGAGLFGLVFMVIIGGVFGFIGGAIVAVIYNIVLGAMGGVELDLEVKT"
     gene            3879..4301
                     /locus_tag="VFC43_01775"
     CDS             3879..4301
                     /locus_tag="VFC43_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HZK29951.1"
                     /translation="MDDLTSDDEPCEQCGLCCRIFGPGITPTAANVFVWIEQGRNDIL
                     RWFVAFMENSTTIPCTDLMPEDLGNLVSVEMRSPDTGEYVTVCPFLRRVAKQRYLCGI
                     HSVKPDMCCTYQPWVWGETYFNRCQALKKTDRKPKWPL"
     gene            4400..4870
                     /locus_tag="VFC43_01780"
     CDS             4400..4870
                     /locus_tag="VFC43_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZK29952.1"
                     /translation="MPPFAYRTTDIRDIDIIRPLWIQLNDYMYTRATTFRSHFEQMTF
                     DKRKAYFEKVSVAGPLRLDLAYADQENGRYVGYCVSSFSAEKTGEIESIFVEDPCRSC
                     GIGSALMTKALAWLDENGSVRNRVSASDGNEGVWEFYKKFGFYPRLTVLEQKRD"
     gene            5134..5814
                     /locus_tag="VFC43_01785"
     CDS             5134..5814
                     /locus_tag="VFC43_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZK29953.1"
                     /translation="MEIIAGIHQVDGVNGNCYILVRDDLTVIDTGLKGSGKKILNYIR
                     KEVHRDPAEIRTIIITHFHMDHVGGVAALKAAAPNIQIAMGSGDEGYVNGTVPLPVYS
                     GLKGTLLHIAGFFMQTGYFTPDLLIHDGDRIAGLLCLAIPGHTPGSIGLLEESKKAFF
                     SGDIFRSDGTTVSGGPLQFTMDQSRENESRKKIAALDFDLLLPGHGVPLRNGASEKVR
                     NFVKYMPV"
     gene            5920..7011
                     /locus_tag="VFC43_01790"
     CDS             5920..7011
                     /locus_tag="VFC43_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HZK29954.1"
                     /translation="MKPVFVKGITNTPSGSSLVSDIEKILLQATDNLAWLKKGDIVLL
                     KPALNSPDPYPSTTHPLALEVTARVLSEHGADVVIGDQSGMEHVVHGPAGVVRGNTCD
                     NYRHSGMAGLNSTPFVRFEGEGWDEGFSHYSSANTKSWKQGFFIASWIKKADHIISLP
                     RISTHAQAGVTLGMKIMVGLLREDSRLEFHANGPYNGTIVRYSKGSDLLSHDDGSGTF
                     FEKIVEISDAVKEKLRLTLFVGTEAQATFGPDRYAYRMGNLGMGEAYRVRLNPALVFA
                     SADQVAAEAFAFALLKDLKRTVPVTAQFKEKILLFQNRFIRDVMDVRVRDHPFIRHGI
                     DIGLGTMPGEIVFSDVPDRFQDRIRKYLV"
     gene            complement(7040..7198)
                     /locus_tag="VFC43_01795"
     CDS             complement(7040..7198)
                     /locus_tag="VFC43_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29955.1"
                     /translation="MKFIWTILILVIVLVVLGLFGNAFVRGQLACRYGKQRETGCPAE
                     QLFDKKRD"
BASE COUNT         2113 a         1903 c         1882 g         1964 t
ORIGIN      
        1 cggcccggag tggtgcacta taatacacaa acacccttcc gtttgttgca aactgcgggt
       61 ggaacgcaag ggacagcagc cctcgctcat cataggtagg atcgatcttt accacccggt
      121 cccggacatc aaggaatggt tgctcaacaa cggtaccatt catgaagaaa atcttcacat
      181 agccggtctg gtcaacggca aaaagcctgc cgctcccatc atgcgggtct gcgatatcca
      241 ggggggctgt gaatcctccc gccacgaatc tgaggcctgc cggaacatcc ggggtatgca
      301 gtctggttaa cgcgggatag gtagtaaatg ttgccgtttc agggcttttc tggtttgtga
      361 gatccggtgt tgcagccggg tttggggtcg gctgctggct tacacacccg gtcaatacta
      421 taatcccggt aagggacaaa accagaagga aaattatcca tttcttatcc atcataatct
      481 ccactcttcg agtattgaca aaaatttctc tcacccttta aaaaattgag tattgttttc
      541 attttttaga acgaatgccg atgaataaag aaaatccttt cgtaccatac tgcttctttg
      601 cagcccggtt ttacctttga taaatcagtg acatgccata atgaacagct gggtgctgcg
      661 gtacagtcat gaacccgggg cttcattgag gaagtttcca aaaaatgaaa actatcgaaa
      721 gggttatgat ccccgcaggg gatttgtctt tgccataaaa ggtgaattct atgaaaaaaa
      781 ctgcattatc attcattctg gtcgttttaa tcgcgacagt ctttataacc ggatgcacac
      841 aacagccggc acaaccgacc ccccagccga ctacggttca acctgccgat acaataaagg
      901 tctctgactc ggtactcggg agaatcctcg ttgacgcaca gggaaagacg ctgtactact
      961 tcgcaaatga cattccctcc agggctaaca gctcctgtta tgggcagtgt gcaattatct
     1021 ggccggtctt ttcgtcagat atcaccgtat ccccgcccct tgtatcttca gactttgcat
     1081 cattcacccg tgctgacggg ctgaaacaga ccgcctaccg cggctggccg ctctattact
     1141 atcagcagga cccaaaaccc ggggatttga acggcgagaa cgtgcttaaa gtatggtttg
     1201 tggtcaagcc ggatgaaacg gtcatgattg cctaccaggg aacggtagga tccttcctca
     1261 cggataaaac aggaaaaact ctctatacct tctcacagga tacccccggg acaagtgcct
     1321 gtaccggagc ctgcctcacg aaatggcccg ccttcaacgc gtcttccatc aatgcaccat
     1381 cagtcctgaa accagccaat ttcagtgccg tgacacgggc tgatggggta ctgcagactg
     1441 cctatatgaa ccgccctctc tattatttcg cggatgatgc aaaacccggg gacgtaaagg
     1501 gacagggttt caataacgtc tggtatgtcg cgaatgttac aggttttgtc ccagttccga
     1561 cgactgtgcc cacgactctt cgcaccctga gctatggtgg cgggtactaa aacaatcata
     1621 caattaagag agaaaaatgt gtcaggaaag gaaccttctt ttccctttcc tcatttaagt
     1681 gacccattgc aaggaaaaca gtagattatt cagctaatgt caaaattttc cggagtattg
     1741 ttattttttg tcagtattgt ttgcagccag gtacagtcca agatacacac aatcctgctt
     1801 ggtaaaggta ggttcatttg caatcaaatc aatgagttct gatgcgggga tatactggcc
     1861 actgccgtgg gcgtccagta tctcagacat acggattatc aactccttat gtcgctgtgc
     1921 attctcgccg gttttttcaa ttttcatacc tgaacaggta atagtgtaat cagggattta
     1981 aaacaatcct gaaaattaaa atcaaaaaaa ataaaagggc tgattatttt tgccctgcat
     2041 tccatgatat tgatagtctt tcgttcaaag gttttgtgca ggcacgcacc ggttttctgg
     2101 cgtttcggca accggtccat gcgggaggtc aatatccagc gcttctttta aaacctcttc
     2161 aatggtctcg actatgacaa aggtaagctc gccccggacc tcttcaggta cctcttccag
     2221 gccccatgca ttctctttgg gaaggatgac ttttttgatg cctgcacggt gtgctgccag
     2281 gatcttttcc ttgatgcccc cgaccggcag gaccgtgccg ctcagcgtga tcttcccggt
     2341 catggcaagt tttggatcca cagtccggcc ggtaattaag gatgtgaggg gcgtaaagaa
     2401 ggttacctgc atattcagag atgtagacct ttctgcttat agcgagattt ttcccatggg
     2461 cttatcgcac atctgatact ttttaccggc tgcttcttga gatctttttc cgtaatgaaa
     2521 tccttaaaca tctccctcac ccatcataat ccggagcgaa aaactatgga taagatcaaa
     2581 ctcggcccga tgccgtacat gagcgttatg cccaccctgc ttgtcggtgc aaatgtcagg
     2641 ggtaagccga actatatgac cgctgcctgg gcgacagtcg cctgcatggc accacctatg
     2701 gtctgtgtcg cgatcaacaa ggcacgttat accgcaaagg ggatcgaaga gaataagact
     2761 ttcagcctga acgtgccgtc tgccagtcag gttgtcgaga ccgaccactg cggtctcgtc
     2821 tccggcagtc ttgaggataa gtccggcgtt tttgagtcat tttacggcag cctgaagact
     2881 gctcctatgg caaaggagtg tccggtctct atcgagtgca cagtgtttaa gtcagtggac
     2941 tgcgggagtc acctgctgca tatcgggcag attgttgaga tatatgctga caaaacctgt
     3001 atgaccgatg ggaaaccgga tataacaaag ataaacccga ttgtatatgc gcaggccacc
     3061 tattttgaca tcgggaaaca ggttgacagg gctttctttg caggaaaagc gtacaggaag
     3121 gagtagctcc ggatatttcc tgaacttttt ttaccgtgta atttcccaaa tttcaactct
     3181 gttgtactcc cgattaaagg atggatgtgt ttaaggagtg gaccgtgcct gtccccccgg
     3241 accgctcctg cgctttacga tactgccatt attgccttct atagtaggta tcgttaggtg
     3301 aattgcggaa ttggctgggg tgaaaccctc ctaaacgttc aggttctttg aaaattttat
     3361 agagccccaa tttcatttcc tgcattgcaa aagttcagaa aattatgtct ttacttccag
     3421 gtccagttcg acacctccca ttgcaccaag gacgatgttg tagatgacgg cgacaattgc
     3481 gcccccgata aacccgaaaa ccccgccaat gatgaccatg aagaccagcc cgaaaagacc
     3541 tgcaccgatt cccagtccat gggagcccag aactgatccc atccctccca gacctgccgc
     3601 aaggaaaatc cctacaaaga aaccccatac aagcccaaag actgcaaaaa acttcccgac
     3661 acttattaca ccgaactgtt taatggtgta cgttgtacct gacataggca atcatactac
     3721 ggtaaaacaa gaaaaagata gcgaaaaaaa gcacttggat ctcatgagtc tttgaggcta
     3781 acatacccat tacatatacc agaatatgac atatccaagc aggattctga taaaaagtaa
     3841 atgtgagaaa tgcgatgtga ataagagagg ggaccataat ggatgacctg acaagcgatg
     3901 atgagccctg cgagcagtgc gggctctgct gccggatatt cgggcccggc atcacaccga
     3961 cagcagcgaa tgtgtttgtc tggatagagc agggaaggaa cgatattctg cgctggtttg
     4021 tcgcgttcat ggagaacagt acaacaatac cctgtactga tttaatgcca gaagatctcg
     4081 ggaacctggt atccgtcgag atgcgcagtc cggataccgg cgaatatgtc actgtctgcc
     4141 cgttcctgcg ccgcgtggca aagcaacgat atctctgcgg gattcattca gtaaaacccg
     4201 acatgtgctg cacctaccag ccatgggtct ggggcgagac ctacttcaac cgctgccagg
     4261 ctctgaaaaa aaccgaccgg aaaccaaaat ggccgctctg atggagaagg acagaccata
     4321 aagacccgct gcgaaaaagc atgcacagac tccctactgc ctgttttttc ttccccggaa
     4381 tcctacaccc aatctgacta tgcctccctt tgcataccgc acgacggaca tcagggatat
     4441 cgacattatc cggccgctct ggatccagct caatgactac atgtatacaa gggcaaccac
     4501 attcaggagc cattttgagc agatgacgtt tgacaaacgg aaggcgtact ttgagaaagt
     4561 ctcagttgcc ggtcccctgc gacttgacct ggcttatgct gatcaggaaa atggtcggta
     4621 tgttggatac tgtgtgagtt ctttctcggc tgaaaagacc ggggagatag aatcaatatt
     4681 tgtggaggat ccctgccgtt catgtggtat cggatcggct ctcatgacaa aggcgctcgc
     4741 ctggctcgat gaaaacggat ccgtgagaaa ccgggtctct gccagcgacg ggaatgaagg
     4801 agtctgggaa ttttacaaaa aattcgggtt ttatccccga ttaaccgtcc ttgaacaaaa
     4861 gcgggactga aggactgcac agattattct cggtcaccaa ccttgtccca ggttgagata
     4921 atccgaaatc gatcgtttca gttttcagat ccggacagat atgcgattta tccggcactt
     4981 tttttttaag ctcaatatcg cttcggccaa gcctcactga taaggattaa aggcttgtga
     5041 ggatatcatc cggaactcaa ttttccacct ggatttgagt caacaggtaa ttttgattaa
     5101 gcagtataac tttatcactc atgcagagga catatggaaa tcattgcggg aattcaccag
     5161 gtggatggag tcaacggtaa ctgctatatc ctcgtccgtg atgaccttac cgtcatcgac
     5221 acgggactga agggcagcgg gaagaagatc ctgaactaca tcagaaaaga ggttcaccgg
     5281 gaccctgcgg agatcaggac aattatcata acccactttc atatggacca tgttggtgga
     5341 gtggcagcac tgaaagcggc agcaccgaat attcagattg ccatgggttc gggcgatgag
     5401 ggttacgtga acggaaccgt gcccctgcca gtctactccg gcttaaaggg cacgctgctg
     5461 catatagccg gatttttcat gcagacaggt tatttcaccc cggatctcct catccatgac
     5521 ggtgacagga ttgccgggct tctctgtctg gcaatacccg gtcacacacc gggaagtatc
     5581 ggtctgctcg aggaatcaaa aaaggcattc ttctctggag atatcttccg ttctgatgga
     5641 acgacggtaa gtggcggtcc gttgcagttc acgatggacc agtcacggga gaatgagtcc
     5701 cgcaaaaaga ttgcagccct tgatttcgat ctcctgctcc ccggccatgg agtgccgctc
     5761 aggaatggtg cctcagagaa ggtacggaat ttcgtaaagt atatgcccgt ctgataaaca
     5821 caaaaaaaat tttccaccac ggaaaaaccc gccggatagg ggtgtatcag gaacaaaggg
     5881 agatattttt taataagagc agccggcatg taatcttgca tgaagccggt ctttgtcaag
     5941 ggaataacga acacaccgtc gggcagttcg cttgtttccg atattgagaa aatactcctg
     6001 caggcaacgg ataatctcgc gtggcttaaa aagggtgata tcgtactgtt aaagccggca
     6061 ctgaattctc ccgacccgta tccctctacc acccaccctc ttgcccttga ggttactgca
     6121 cgtgttctct cagaacatgg tgcggatgtt gttatcggtg accagtcagg aatggaacat
     6181 gttgtccatg gtccggcagg agttgttcgg gggaatacct gtgataatta ccggcattcc
     6241 ggtatggccg gtctgaacag tacacctttc gtgagatttg aaggcgaagg ctgggatgaa
     6301 ggtttttctc attattcgtc agcaaacaca aaatcctgga aacagggttt ttttattgct
     6361 tcatggataa aaaaggctga ccatattatc agcctcccgc ggatctcaac gcatgcccag
     6421 gccggtgtta ctcttggcat gaaaataatg gtgggattac tcagggagga cagccggctg
     6481 gaatttcatg caaacggccc ctataacgga actatcgtcc gatattcaaa agggagcgat
     6541 ctcctgtcac acgacgatgg ttcgggaact ttttttgaaa agattgttga aatttccgat
     6601 gcagttaaag aaaaactcag gcttaccctg tttgtcggga ccgaagcgca ggccaccttt
     6661 ggccctgacc ggtatgctta ccggatgggt aaccttggaa tgggagaggc gtaccgggtc
     6721 agactcaacc ccgccctcgt atttgcaagc gccgaccagg ttgctgctga ggcttttgcg
     6781 ttcgctctgc tcaaagactt aaaaaggact gttcctgtta cagcacaatt caaggaaaaa
     6841 attcttctct tccagaacag gtttatccgg gacgtcatgg atgtcagggt gagggatcac
     6901 ccgtttatca ggcatggaat cgatatcggt cttggtacta tgcccggtga aattgttttt
     6961 agtgacgttc ctgaccgttt ccaggatcga atcaggaaat atcttgtgta ggtaatgaat
     7021 ccgcctgaat aagagaaatt cagtctctct ttttatcaaa taattgctct gccggacaac
     7081 ctgtttctct ctgtttacca tatctgcacg ccagttgtcc ccgcacgaat gcattgccaa
     7141 acaaaccaag cacaacgagg acaatgacaa gaatgaggat tgtccagatg aatttcatga
     7201 ccagaagtac aatccctgcg aggaccggga tgaaaaacag taaaaaatcc gggataatat
     7261 ctttccatgt gatcgttttt ttgcagaatt gtttgagggt ttcctttggg aaataacctg
     7321 ctgctcagcc atcctttgcc aaaggcatac cgtttgccat aataataaca gtccacacaa
     7381 tgcccgctca ggaggcggaa ttcacacata aggatgaaga ttatatagag aataacccag
     7441 atcaggccaa acctgaacag cacatatgca ccgatactgt aaatcaggag ggacaggaga
     7501 tttgaaacga aaataaccct ggtcggatat ttttcataac agccgggatc ctgcatacca
     7561 gaatagaata atgtaaaaaa tatgaaataa cttccgtaaa ccacggtagt atcagattga
     7621 cgcggataac ctcagacgca aatccctgat ggtcgttgaa aatttacagg tcatgtactg
     7681 tccccagcgg tacttgcaat gagctaaaga gactgcgtga gaaaacggca gtacaggaac
     7741 gagaggttcc tgcgccccag aaagcgcgga cgtaaaatat gaatccgttc ccacgcaaca
     7801 acggcgtgaa tgaaacgtgc ctgttcattt gccgtaaatg acaatggaga gaatatgaat
     7861 gt
//
LOCUS       DASRLV010000045         3906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_823768,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000045 DASRLV010000000
VERSION     DASRLV010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823768"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="VFC43_01800"
     CDS             <1..1048
                     /locus_tag="VFC43_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZK29956.1"
                     /translation="LYSSATAVVFPSDHILGESALDQIRASEPLARKYLVTFGIKPTS
                     PQTGYGYIKQGKALLKGAIVDEFKEKPSETTAKEYVKRGYLWNSGIFLLSTDCFFQEL
                     KKYRPDLFHAFAEGHVADYRKLESISIDYGLLEHSKKVSVVTLETEWNDLGTFKALYD
                     SELHDSEGNVGIAEYFSATNNFVQAPGKHVGLIGVHDLIVVDTSDALLICDNSQTEQV
                     KQLVNRYNERKDPVTKFHRQVHRPWGSYTVLEETDIYKIKRVSVKPGHRLSLQLHHHR
                     SEHWVVVSGTAEVELDGEARFLRQGESTFVRSGMRHRLKNPGIIPLEVIEVQLGEYLE
                     EDDIVRIDDEYGRK"
     gene            complement(1137..2981)
                     /locus_tag="VFC43_01805"
     CDS             complement(1137..2981)
                     /locus_tag="VFC43_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HZK29957.1"
                     /translation="MTPQPDFLPLSGTERNKLGIPQFDVILVTGDAYIDHPSFGTALV
                     GRVLWDAGFTVGIIAQPDWKNAADFMVLGKPRLFFGISSGNVDSMVNNFTPNLKRRGD
                     DVYSPGGIHKRPDRATIVYTNRVHELFPGSLIIIGGIEASLRRFAHYDYWQDRVRQSL
                     LADAPADLLIYGMGERQVVEIARRLASGESISTIRNVRGTAFGMEIAEWRSKKPEGIV
                     ELPGFSEVTTDKKSYARAFAMHYHEQDPVRGRPIAQPHPKMVVIQNPPALPLSTEELD
                     HIYDLPFSRRAHPSYKERIPALETVQFSVTSHRGCFGACSFCALTHHQGRILQSRSSE
                     SIIREVTKMTRMPEFKGIVQDVGGPTANMYCLSCSKWEKYGACRDKHCSTSCKNLNTS
                     HRYQVDLLKRLREIPGVKKVFIGSGIRYDLAMEDDSGYLEVICNHHVSGHLKVAPEHV
                     TEKVTVAMNKPKREVFDRFCERFELLQKGKKKRQYILPYFMSGHPGCTINDMIDLALY
                     IRDHSLYTEQVQDFTPTPMSVSTCMYYTGLNPFTLEPVHVPKGLEKKIQRAMMQYRDP
                     KNRKLILEGLKSAGRTDLIGNGRQFLIPSGSKDTNQYALKHTRNEKRN"
     gene            complement(3029..3619)
                     /locus_tag="VFC43_01810"
     CDS             complement(3029..3619)
                     /locus_tag="VFC43_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HZK29958.1"
                     /translation="MQYTEGQLGRVFVVRIDNGEDMLISLRQFIEDKSIQAGSILFLG
                     ALMNGRMVTGPEEPVIPPVPHFVMFEGGWEVFGVGTIYAGEGGPHIHYHASVGRSGHA
                     LTGCLREKATTYLIIEAVILEFTGLTARREFDKKTQVHLPVLGKDVNNESSGTTENMD
                     SHEAIEKSPANEEEPADDLPDGLADMIRDLTGNYNS"
BASE COUNT         1052 a          922 c          929 g         1003 t
ORIGIN      
        1 cctttattcc tctgctactg cagttgtatt tcccagcgat cacattttag gagagtccgc
       61 actagatcag atacgggctt cagaacctct ggcgagaaaa taccttgtca cgtttggaat
      121 aaaaccgacc tcgccccaaa cgggttatgg gtatatcaaa cagggaaaag cccttttaaa
      181 aggggcaatt gttgatgagt tcaaagaaaa acctagtgaa actacagcaa aagaatatgt
      241 caaaagaggc tatctatgga atagcggcat atttcttctc tccaccgact gcttttttca
      301 ggaactgaaa aaataccgtc cggatctttt ccatgcattt gccgaaggac acgtggcaga
      361 ttaccggaaa cttgaatcga tatcgataga ttacggactt ctcgaacatt caaaaaaggt
      421 gtctgtagtt actcttgaaa ccgagtggaa tgacctgggt acatttaaag ccctgtacga
      481 cagcgagctg catgacagtg agggcaatgt tgggatcgcg gagtattttt cggccacgaa
      541 taattttgtc caggcacctg gaaaacatgt aggactgatt ggagttcacg atctcattgt
      601 ggttgacacg tccgatgctc tccttatttg cgacaacagc cagacagagc aggtaaaaca
      661 actcgtaaac cgctataatg agcgcaaaga tcctgttacc aaattccacc ggcaggtaca
      721 tcgcccatgg ggatcgtata cggtgctgga agaaacggat atctacaaaa tcaaacgggt
      781 gagcgtaaaa cccggtcacc ggctgtccct ccagctgcac catcaccgga gtgaacattg
      841 ggttgttgtc agcgggactg cagaagtcga actggatgga gaagcccggt tcctcaggca
      901 gggggagagc acgtttgtcc gcagtggtat gaggcatcgt ttaaaaaatc cgggtattat
      961 tccccttgaa gtcatcgaag tccagctcgg tgaatatctt gaagaggatg atattgtcag
     1021 gattgacgat gagtatggaa ggaaataaaa aacctccctg aaaatttttc tcaattaaag
     1081 aaaaccagaa taaaaaaaac cagaataacg tgatccggtt tacttgccac agtgtttcaa
     1141 tttctttttt cgtttcttgt gtgttttaga gcgtattggt tagtatcctt actgccagag
     1201 ggtatcagga actgcctgcc attaccgata agatcggttc ttcctgcgga ttttagccct
     1261 tcaagaatta atttccggtt tttaggatca cggtactgca tcatcgcccg ttgtatcttc
     1321 ttttcaagtc ctttgggtac atgaaccggc tcaagggtga aaggattcag gccggtataa
     1381 tacatgcatg tcgaaacact catcggggtg ggtgtaaagt cctgcacctg ttcagtataa
     1441 agagagtggt cacgaatata gagtgcaaga tctatcatat cgtttatcgt gcagccggga
     1501 tgtcccgaca tgaaatacgg gaggatatac tggcgtttct ttttaccctt ttgtaatagt
     1561 tcgaaacgtt cgcagaaccg atcaaaaact tcacgtttgg gtttattcat tgcaaccgtg
     1621 accttttccg tcacatgctc aggagcgacc ttgagatggc cggaaacatg atgattacat
     1681 atcacttcaa ggtagccgga atcgtcttcc attgcgaggt catagcgaat accggaaccg
     1741 atgaagactt tttttacccc gggaatttcg cggagccttt tcagaaggtc aacctgatag
     1801 cggtgacttg tgttcaggtt tttacaagaa gtactgcagt gtttatcccg acaggcacca
     1861 tatttttccc actttgaaca cgaaaggcag tacatgtttg ctgtcggacc accgacgtcc
     1921 tgaacgatgc ctttgaattc gggcatccgg gtcatttttg ttacttcacg gatgatcgat
     1981 tcactgcttc ggctctggag aatgcgtccc tgatgatggg ttaatgcaca gaatgaacag
     2041 gcaccaaaac acccccggtg actcgtgaca gagaactgaa cggtttcaag ggcagggatt
     2101 cgttctttat atgacggatg cgcacggcgc gaaaacggca gatcatagat atgatcgagt
     2161 tcttcggttg acaatggcag agcaggagga ttctggataa caaccatctt cggatgcggt
     2221 tgtgctatcg gcctgccgcg aacagggtcc tgttcgtgat aatgcatggc aaaggcccgg
     2281 gcataggatt ttttatcagt tgtaacctct gaaaaaccag ggagttcaac aattccctcc
     2341 ggctttttac tgcgccactc tgcaatttcc ataccgaaag ctgtaccgcg gacattccgg
     2401 attgtagaaa ttgattcacc ggaagcaaga cgccgggcta tctcaacgac ctgtcgctcc
     2461 cccataccgt atataagaag gtctgccggg gcatcagcaa gaagagattg ccggacccga
     2521 tcctgccagt aatcgtaatg ggcaaacctt ctcaggcttg cctcgatgcc cccgatgata
     2581 attaaggagc cgggaaacag ttcgtggacc cggtttgtgt agacaatagt tgcccgatcg
     2641 ggacgtttgt ggatcccgcc gggagaatag acatcatcac cacgccgttt aagattggga
     2701 gtgaaattat tcaccatgga gtcgacattt cccgaagata ttccgaagaa cagtcgtggt
     2761 tttcccaaca ccatgaaatc agccgcattc ttccaatcgg gctgggcgat gataccaaca
     2821 gtaaaaccag catcccaaag gacacgacca accagtgcag taccaaagga gggatgatcg
     2881 atatacgcat ctccggtcac caggatgaca tcaaattgcg ggataccaag tttatttctc
     2941 tctgtccctg acaagggaag aaaatctggt tgcggagtca tgaagattga ggggtcaggt
     3001 gattgtctga tggccaaaaa aacatagttc acgaattata attgccagtc agatcacgga
     3061 tcatatccgc aagtccatct ggcagatcgt ccgcaggttc ctcttcattg gcaggggatt
     3121 tttcaatggc ttcatgcgaa tccatgttct cggtagttcc tgatgattca ttattcacat
     3181 cctttcccag taccggaaga tggacctggg tctttttatc aaattctcgt cgtgcagtaa
     3241 gaccggtaaa ttctaaaatc accgcttcaa taataagata cgtggttgct ttttccctga
     3301 ggcacccggt aagggcatgc ccggaacggc caaccgaggc atgataatgg atatgagggc
     3361 ctccttcacc tgcatatatg gttcccaccc cgaagacttc ccagcccccc tcaaacatca
     3421 cgaaatgagg gacggggggt attaccggtt cttcaggtcc ggttaccatt ctcccgttca
     3481 taagagcacc gaggaagagt atcgagccgg cctgtataga tttgtcttcg ataaactgac
     3541 gcagcgaaat caacatatcc tcgccgttat cgatcctcac gacaaaaacc ctgccgagct
     3601 gaccttccgt atattgcata tattcagatt ccttgtttgg tattcatatg gtttgtgcgg
     3661 attaatacct ttttttggca cttaaatagc ggatgttcgc cgcaaaactt caaaaaatgg
     3721 aaatgataac tgattttggg atcatgagtt cggggagatt tgttcattat ctcccgccag
     3781 caccgccgcc accaaatcct cctccacccc caaaacctcc accaccgcca aagccccctc
     3841 cgccaccgga tcctccatgg cttggggggg tgaagtgaag caggggaaga aatgcataat
     3901 tcatac
//
LOCUS       DASRLV010000046         4288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_179551,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000046 DASRLV010000000
VERSION     DASRLV010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179551"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VFC43_01815"
     CDS             <1..340
                     /locus_tag="VFC43_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZK29959.1"
                     /translation="VMAYASTEEKERLMNRAKCIICRSGYTSIMEIAELGKKHALFIP
                     TPGQTEQEYLSWYYEKKSWFHSKSQYHLNLTEDIRVAHDPRYTGFTKSGRTRENVNNL
                     YDQVLAQYLE"
     gene            403..621
                     /locus_tag="VFC43_01820"
     CDS             403..621
                     /locus_tag="VFC43_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29960.1"
                     /translation="MDDLVLLGAAGILGLFLAAVILLFGIYSISGLMYAAFLSGSYDV
                     LFIITGLILIVVSSYIATGLWLHKTDRI"
     gene            complement(773..1021)
                     /locus_tag="VFC43_01825"
     CDS             complement(773..1021)
                     /locus_tag="VFC43_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29961.1"
                     /translation="MGIAPVTRDWPSQCAGAGITVTELIVMVEQNSGGMKLYARSSFV
                     TISYENHCSSDTILELLHFNQSVMMNAAAPKRGAPAAD"
     gene            1129..1566
                     /locus_tag="VFC43_01830"
     CDS             1129..1566
                     /locus_tag="VFC43_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HZK29962.1"
                     /translation="MDTTDGLELSAKKVEYLKYLFGQEYFVKTNNLASRFGVDPSTIT
                     KTISELAESGYITHTPYHGVCLSEKGMRYAEFCIKRHRILALMLTHYGLSHELACTEV
                     SRFEGFVSRDAIDRICHAMGHPRQGVCGEIVQDTACPECDEPE"
     gene            1608..2435
                     /locus_tag="VFC43_01835"
     CDS             1608..2435
                     /locus_tag="VFC43_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HZK29963.1"
                     /translation="MTTTSLSDHIPDLNLITFYAQNRQTVFSRVSPWTKLSLLILVIV
                     FITVMKNIFLIGGLYTLVLLVYRGAGLPLKKLFAWYALPAVFVLSFVGILAWSEPGHA
                     IISFSIAGMPFMLTDNGIMLIALLLLKALTVVTWSLFFLMTTRYEHFAAMIYRIFPAP
                     LDQIFLMAYRFLFLTLAMTASILKAVRSRGGGLIQSVRMQGRLFADIFALVFIRSFER
                     SERVHKAMAARGFSGSYGTAVEIPALRVYEYVLIGICTALVIAFIIRDPVTGGILLL"
     gene            2432..3340
                     /locus_tag="VFC43_01840"
     CDS             2432..3340
                     /locus_tag="VFC43_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZK29964.1"
                     /translation="MTIIRTNPAGISHCPHTDRERELIHVDCASHVYPDGSVGIHNMC
                     FYVQKNEIVALCGPNGSGKSTLIEHLNGLLTPTEGNIAVNGRSITTGEHTNLWREVGI
                     VFQNSDDQLFAPTVLDDVMFGPLNMGMSRDAAKTAAGEALEAVGASDLVNKLPNYLSG
                     GQKRLVSIAGILAMKPRIIALDEPTSDLDTVHSSMVEHLILDLKNRFGISIVIATHDL
                     DLAARIADRICLVRQGSVFAEGPPHEIFYDSELLQENGLRPPQIVQVYRAYCQTMGIT
                     EDRNPLTIDAMMEVIHRSIGARKEDA"
     gene            3439..4098
                     /locus_tag="VFC43_01845"
     CDS             3439..4098
                     /locus_tag="VFC43_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HZK29965.1"
                     /translation="MAHIHLEDGSFTLFWAIAWWVIALLLVSAALFWVRSVKKIDNRK
                     ITLAAFCTAAAFTVFQVSIPVLGGVHLNLTPLIGILVGPAAGSLVVLIVNILSAAVGH
                     GGWGMIGANLLVNIIEVVVAYTLFRVTKKIFSGAFARGGIATFTSLFCGNLVMIAIIL
                     VSEIQGVTQTKAQILAGLSLLAGINMGVAIIEAVITGMIIAYMVRVRPDMLENEQSKK
                     G"
BASE COUNT         1072 a         1015 c         1018 g         1183 t
ORIGIN      
        1 ggttatggca tatgcctcca ccgaagagaa agaacgctta atgaaccgtg cgaaatgtat
       61 catctgtcgt tcgggataca cgtcaataat ggagatagct gaactgggaa agaagcatgc
      121 cctgtttatc cccacacccg gacagactga acaggaatac ctgtcatggt attatgagaa
      181 aaagagctgg tttcattcaa agagccagta ccatctcaac cttacggaag atatccgggt
      241 tgcacatgac ccccgctata caggatttac aaaatccggc cgtacccggg aaaatgtcaa
      301 caatctctat gatcaggtgc ttgcacagta cctggaataa tgcgtgtttt tagcaaaaaa
      361 caaaacttaa acagtttata gacgaaatgg tcactactga gtatggatga tctggttctt
      421 ttgggggctg caggtattct cgggcttttt ttagcagcgg tgatccttct tttcggcata
      481 tactccatct ctggactgat gtatgccgct ttcctgtccg gatcctatga tgtgcttttc
      541 atcatcaccg gactcatttt gattgtcgta tcctcgtata ttgcaaccgg attatggctg
      601 cataaaacag acaggatctg aaatatgctt ttgtgtaatt atttaaaaag gtctgttgaa
      661 cagacatgaa cttattgcgt tcacacgtaa atcacgataa tcgtttttcg aaacgatgga
      721 cccctgcctc agggcctctc tttaggcacg gcgggcaggg agtttttatt cattaatccg
      781 ccgcgggggc gccccttttt ggtgcggcgg cattcatcat tactgattga ttgaagtgta
      841 acaattccag aatcgtatct gatgaacaat gattttcata ggaaatggtt acaaaacttg
      901 aacgtgcata gagtttcatc cccccgctat tctgctccac cattacgata agttctgtga
      961 cggtaatacc agctccagcg cactgcgagg gccagtctcg tgtaactggc gcgattccca
     1021 taaagcatct gtgctttatt gatttgtttt tcctggttgt acctgaatcc gggaaaattt
     1081 ggtatcctgc caaattttat taaatatgga tgctccatac tgaacgatat ggatacaacc
     1141 gacggtctcg aactctcggc aaaaaaggtt gaatatctca agtatctctt cgggcaggaa
     1201 tattttgtaa agacgaacaa ccttgcctca cggttcgggg tcgacccttc aaccattaca
     1261 aaaactatca gcgaacttgc agaatccggg tatatcaccc atacccccta ccatggggtc
     1321 tgcctttcag aaaagggcat gcggtatgcg gaattttgca taaaacgaca ccgtatactt
     1381 gcactcatgc tgacccatta cggtctctcc catgagctgg catgcaccga agtctctcgg
     1441 tttgaaggct tcgtatcccg cgatgccatc gatcggatct gccatgcaat gggccatccc
     1501 cgtcagggag tctgcggcga gatagtccag gatactgcat gtcccgaatg cgatgaaccg
     1561 gaatgataaa aaatgaattt cttgtatgaa aacagggagg acaatttatg accaccacat
     1621 ctctttctga tcatattcct gacctgaatc tgatcacctt ttacgcacaa aaccggcaaa
     1681 ccgtattttc acgggtaagt ccatggacaa aactctcgct gcttattctt gttatcgttt
     1741 ttattaccgt aatgaagaat atcttcctga ttggtggact gtacaccctt gttctcctgg
     1801 tatatcgggg agcgggtctt cctttaaaaa aactgtttgc ctggtacgct ttgcccgccg
     1861 tattcgtgct ttcatttgtt ggcatcctgg catggagcga accgggacat gcgataatct
     1921 cctttagtat agccggaatg ccctttatgc tcactgacaa cggcattatg cttattgccc
     1981 ttttattgct aaaggcgctg acggttgtca cctggtcatt atttttcctc atgacaacac
     2041 gatacgagca ctttgcagca atgatctacc gtatttttcc ggcccccctt gaccagatct
     2101 tcctcatggc ataccggttc ttattcctaa cgctggccat gaccgcttcc atactcaagg
     2161 cagtacgttc ccggggcgga gggctcatac agagtgtgag gatgcaggga cggttatttg
     2221 ccgatatttt tgccctggta tttatccgtt cattcgaacg gtctgagcgg gtacacaagg
     2281 caatggcggc caggggtttt tcaggatcgt atggaactgc agttgagatc cccgcgctcc
     2341 gcgtgtatga atatgtcctc atcggcattt gtaccgcgct ggtaattgct tttatcatca
     2401 gggatcctgt caccggaggg attctcctcc tatgacaata atccggacca acccggcagg
     2461 catttcccat tgtccccata ctgaccggga acgtgaattg atccatgtgg attgtgcaag
     2521 tcacgtgtat cccgatggta gtgtcgggat tcacaacatg tgtttctatg tccagaaaaa
     2581 cgagattgtg gctttgtgcg gacccaacgg gtcaggaaaa tccaccctca ttgaacactt
     2641 aaacggcctg ctcacaccca ctgagggtaa tatcgccgtg aatggaaggt cgatcaccac
     2701 gggcgaacat accaatctct ggcgggaggt tggaatagtc tttcagaact ctgatgacca
     2761 gctctttgcc ccgacggttc ttgacgatgt gatgttcggc cccctgaata tgggcatgtc
     2821 ccgcgatgca gcaaaaactg cagcaggtga agcccttgag gccgtgggag cttctgacct
     2881 tgtcaataaa ctgcccaatt acttgtcggg aggtcagaaa cggcttgttt ctatcgccgg
     2941 cattcttgcg atgaaacctc gcattatcgc actggacgag cctacatccg atctcgatac
     3001 cgtccacagc agcatggtcg aacacctgat ccttgacctg aaaaaccggt tcgggataag
     3061 tatcgttatc gcaacccatg accttgacct ggctgcacgc attgcagacc gaatctgcct
     3121 ggtcagacag ggatcagtat ttgccgaagg gccaccgcat gaaatatttt atgattcgga
     3181 attacttcag gagaatggat tgagaccacc ccagattgtc caggtatacc gtgcatattg
     3241 ccagacaatg ggaataacgg aagatcgcaa tcctctgact attgacgcaa tgatggaggt
     3301 aatccaccgc agtattggtg cccggaaaga ggatgcataa tttggagtga ggccaaaaat
     3361 ttgattaagt atttatttca cgcatccctt catctatcca gtgaatgttg gggtatatca
     3421 tgaaacaggg gtattgatgt ggcgcatata catctcgaag atggatcctt tactctgttc
     3481 tgggcaatag cgtggtgggt cattgctctc ctgctggtat ctgccgccct tttctgggta
     3541 cgatcagtca aaaaaatcga caaccgcaag ataactctcg cggcattctg tactgctgct
     3601 gcatttacgg ttttccaggt gagtattccc gttctggggg gagtgcacct gaatcttaca
     3661 cccctgatag gaatacttgt cggcccggca gcaggttctt tagttgtcct gattgtcaac
     3721 attctttcag cagccgtggg tcacgggggc tgggggatga tcggggcaaa cctgcttgtc
     3781 aacattattg aagttgtcgt tgcatatacc ctgttccggg tcacaaaaaa aatcttttca
     3841 ggagcttttg cccgcggagg cattgcaaca tttaccagtt tgttttgcgg caatcttgtg
     3901 atgattgcca ttatcctcgt ttccgagatc cagggggtta cccagacaaa agcccagatc
     3961 cttgccggac tctccctgct ggccggcatt aatatgggtg ttgcgattat tgaagcagtc
     4021 atcaccggta tgattattgc ctatatggta cgggtcagac cggatatgct ggaaaatgag
     4081 caatcaaaaa agggataatg acaaaaacgg gtgatccctg ctgcgattgt acgaaaagga
     4141 accgggaatt ttccagagag ggtaaaggaa tccccgatta aaagggtgtt ctggtgttaa
     4201 tgcggggatt tcctaagcaa taacttcatt ttgtgaataa taaagtctgg caaggcgctg
     4261 ttgcattgca tttctggttt aaaaacct
//
LOCUS       DASRLV010000047         5512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_748955,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000047 DASRLV010000000
VERSION     DASRLV010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748955"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VFC43_01850"
     CDS             complement(<1..303)
                     /locus_tag="VFC43_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HZK29966.1"
                     /translation="MTSLLSPYKLGNLLLSNRMVMAPMTRCRAIDGNIPGPLSVIYYS
                     QRSSAGLIITEGSQVSPMGVGFDRTPGIYSPDQVNGWKNVTSAVHEKGGKLFLQLWH"
     gene            complement(311..1783)
                     /locus_tag="VFC43_01855"
     CDS             complement(311..1783)
                     /locus_tag="VFC43_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZK29967.1"
                     /translation="MACILVSACIGNQENSAPVIPSPTTANMPVNGTSIDLTNQNSLQ
                     AATFREGGSHTELVSPQPLIRLEKVAGGFSSPMMIAVPPDGTGRLLVVDQIGVVRILT
                     PDKTVPDTPFLDLRDRMVRLSPGYDERGLLSLAFHPDYKNNGRVFVYYSSPLQSGAPA
                     GWSCTNRLSEFRVTAGNPDKIDMNSEKVLLTVDKPFQNHNGGPLLFGPEDGYLYLALG
                     DGGRSDDTGTGHTPGTGNAQDRTKLYGKIIRIDVDSPGAGEKMYAIPPDNPFVTTPGY
                     APEIYASGFRNPAYLSFDAGGNHSLITAVAGQALFESVFIVDKGGNYGWNIREGTHCF
                     NPADDSKPPVSPCPVTGSHGEPLIGPIIETGHDVGNVIVGGFIYRGTALPELSGKYIF
                     GEWSTSFTRGDGILLVSTPPPGFEIGMYPRSVANITPADNAMWTTQEFRVTNNPDGRV
                     NAYVTGFGEDASLELYLLTSTKSGPDPAGSTGEIWKLVKG"
     gene            2155..>5512
                     /locus_tag="VFC43_01860"
     CDS             2155..>5512
                     /locus_tag="VFC43_01860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZK29968.1"
                     /translation="MHELKPGDKKNLWLAGFPEMNPNPVIEMDAQGVITFANTATLNI
                     LKKLGLPQDPAIFAPDDKSEILNLLKDSSEQQVYREIHLKDAWFSENIAFNHELQVVR
                     IYTSDITDRKRIEEELHRAYEHTSTILESISDSFIALDKDWRFTYINRRSSEYSHKNP
                     KDVLGKTVWEVFPEICGTPLETFYKNAMISKEPLVFTNPSEVAAGEHFELYAYPMKDG
                     LTIFGHNITGRKQAEDALRLSEERFRNIVDATFEGLLIHRDGIITDVNRTLTELIGYQ
                     RDEIIGREILGFVDPSYHALMKKGLRIPYEIEIFHRNGTRIPVEVIVKQFTDPNHMIA
                     AVRDITERKRSEESLRKVQMRTTSILENIADTFYSLDKQWRFTVVNPAAETAPFNRPA
                     SEMLGKVIWDLYPELTGTRIQQHYFDAMEKHSMERYVALSPLNDRWYEVFMQGESDGV
                     DVYMRDVTDLKRSEEILRVSEEKYRGLFENVQESVAIYRMVYDENGKAVDRIFVDANP
                     KALSQMGDLRREDVIGKAYSEVVSRKFPKDKISIDKHLQSLAAVVQSGIPVTYDTHFG
                     GKYYLTTQYSLHKDLVASSSIDITERKKAEEALRESEERYRVLVENANSIILKMDKEG
                     KISFFNEYAQKFFGYSLDEIIGQDVKLLVPPTDRSGRSPEEMIDAILTDPDAFAENEN
                     ENIRKNGERVWISWRNRAIRDSLGNLIGNLAIGQDITERKQAEEALRTSEQRFRLALK
                     NAPVSVAVQDTNLIYLWAYNDGTKKSEDIQGKTDTDIFTPEDAAMLTGLKRKVIETGK
                     EAHAQIWLTVNEKRVYYDMYLEPLRNDTGQITGIGTATVDQTLEKLTDIALRENEKRL
                     REANDLLEAVTDATKVIIAAEDTDFRYTYFNKTYAAIIRRLTGKEIMIGMSMIDLFSD
                     LPGEQRSELDEWGKVLNGKSVNDHITFDDPVKGQTIYNVIHVPLRNAKWEIIGAGEIA
                     FDVTTQVQVEETLREASQYLTNLIDYANAPIIVWDPEFRITLFNHAFEHLTGRKAKEV
                     VGHHLEMLFPENYLAPAMDLIKKTSEGERWESVEIPILQKTGEIRTVLWNSGTIFGSD
                     GKTIIATIAQGQDITDRKKIESEYRHRAAEY"
BASE COUNT         1564 a         1304 c         1386 g         1258 t
ORIGIN      
        1 atgccacagc tggagaaaca gtttcccgcc cttttcatga acagcgctgg tcacgttctt
       61 ccacccgttc acctggtcgg gagaataaat accgggagta cgatcaaacc cgacacccat
      121 cgggcttacc tgtgaccctt cagtaataat cagccctgcc gaagatctct gggaataata
      181 gataaccgat aaaggacccg ggatattgcc gtcgatcgca cggcagcggg tcatgggtgc
      241 cataaccatc cggttggaga gtaagaggtt acccagttta taaggagaaa gcaggctcgt
      301 catggttatc ctatcctttg acaagtttcc agatctctcc cgttgaccct gcgggatcag
      361 gaccgctttt cgtgctggtc aggagataca gttcaagaga tgcatcttct ccaaaacctg
      421 tcacgtatgc attcacccgc ccgtccggat tgttcgtaac cctgaattcc tgtgttgtcc
      481 acatcgcgtt gtcagccggg gtgatgtttg cgactgatcg ggggtacata ccgatttcaa
      541 atccgggtgg cggggttgag actaaaagaa taccatcacc gcgggtaaat gatgtgctcc
      601 attccccgaa aatatatttt ccggaaagtt ccggcaaggc agtaccacgg tagataaaac
      661 ctccgacaat cacatttccc acgtcatgac cggtctctat tatggggccg ataagcggtt
      721 caccgtggga ccccgtaacc gggcaggggc tgacaggggg tttcgagtca tcggcaggat
      781 tgaaacaatg cgtcccttcc cggatattcc agccgtaatt gccccctttg tccacgatga
      841 acaccgattc aaagagtgcc tgtcccgcaa cagccgtgat aagggaatgg tttccccctg
      901 cgtcgaacga caggtatgca gggttccgga accccgatgc ataaatttcc ggtgcatacc
      961 cgggcgttgt gacaaacggg ttatccggtg gaatggcata catcttctct ccggcacccg
     1021 gtgaatccac atcgatacgg atgatttttc catacagttt tgttctatcc tgcgcatttc
     1081 ctgtccccgg tgtatggccg gttccggtat catcagacct gccaccgtcg ccaagcgcca
     1141 ggtacagata cccgtcttcc ggcccaaaca ggagcggacc gccattatgg ttctggaagg
     1201 gtttgtcaac agtcagcagg accttttccg agttcatatc aatcttatcg ggatttcctg
     1261 ccgtgacacg gaattcagaa aggcggtttg tgcagctcca gcccgccggc gcccccgatt
     1321 gcaggggtga agaatagtat acaaagacgc ggccgttatt tttataatcc gggtgaaagg
     1381 caagggagag aagaccccgc tcgtcgtagc cggggctcag gcgtaccatg cgatcccgga
     1441 gatcaaggaa cggcgtatcg ggaactgttt tatccggagt aagtattctg acgacaccga
     1501 tctggtcaac gaccaacaag cgtccggttc cgtcaggagg gacagcaatc atcatggggg
     1561 aggaaaagcc accggcaact ttttcaagcc tgataagcgg ctgaggagag acaagttcgg
     1621 tgtgagaccc cccctccctg aatgtggctg cctggagaga attctggttg gtcagatcga
     1681 ttgaggttcc gttcaccggc atatttgccg tggtgggaga aggaataacc ggagcactat
     1741 tctcctggtt tcctatacag gcagacacta aaatacatgc caccagaaag gcagaataaa
     1801 ccagcaactg aaaatttttc ataaagtctt ctcagaaaaa cgattcctta atcatttggg
     1861 tacattccag attctgtaag gacattaatt tttacggttg tcaggaaaca atccagccgt
     1921 ttaccttcaa ccagatatcc ttatccgatg tttcaaaaat tgtaaagtgg gggtatacaa
     1981 atattcaaaa aagtttttat tatctgtata tatcctgaaa atgaacatga aacggtgatt
     2041 taaaaagtat ccggtgggga tattagctgg agaaggaagg gccacaaacc ctggttttat
     2101 aatttaatac actatctgag acgacataat taaaggtgac aaggagtaac atttgtgcac
     2161 gaactcaaac ccggagacaa aaagaatctt tggctggctg gtttccccga gatgaacccg
     2221 aacccggtta tcgaaatgga tgcgcaaggg gttatcacat ttgcaaatac tgcgacgctg
     2281 aatatcctga aaaaattagg tcttccccag gatcccgcga tatttgctcc ggatgacaaa
     2341 tcagagatct taaatttatt aaaggattct tcagaacagc aggtttaccg ggagatccat
     2401 ctgaaggatg catggttttc ggaaaatatt gcattcaacc acgagttaca ggtagtcaga
     2461 atttatacct cggacatcac cgaccgtaaa cgaattgaag aagagttgca tcgcgcttat
     2521 gaacacacct ccactatcct ggaatccatc agtgatagtt ttattgccct tgataaagat
     2581 tggcgtttca cctatatcaa ccggcgatcg tctgaataca gtcacaaaaa tccaaaagac
     2641 gtgctgggaa agaccgtctg ggaagttttc cctgaaattt gtggtacacc gctggaaaca
     2701 ttttataaaa atgcgatgat cagcaaggaa cccctggttt tcaccaaccc gagtgaggtt
     2761 gctgccggag agcatttcga attatatgcg tacccgatga aagatggtct gacaatcttt
     2821 ggccataata tcaccggacg caaacaagcc gaagatgctc tccgtttaag tgaagaacgc
     2881 ttccgcaaca ttgttgatgc aacgtttgaa ggactcctca ttcaccgtga tggaataatc
     2941 acagacgtca acaggacgct gacagaactt atcggttatc aaagagacga gatcatcggc
     3001 agggagatcc tcggatttgt tgatccgtct taccatgcat tgatgaaaaa gggactccgg
     3061 attccgtatg aaattgaaat ctttcatcgt aatggtacca ggatcccggt tgaagtgata
     3121 gtgaaacagt tcaccgatcc aaaccacatg atcgctgcgg tgcgggacat caccgaacga
     3181 aaacggtcgg aggagagtct gcgtaaggta cagatgagga ctaccagtat attagaaaac
     3241 attgcagaca cattctattc tctcgataaa cagtggcgtt tcaccgttgt gaaccccgca
     3301 gccgagacgg ccccctttaa ccggccagcc tccgaaatgc tcgggaaggt catctgggac
     3361 ctgtacccgg aactgacggg aacacgcatc caacagcatt atttcgacgc tatggagaag
     3421 cattccatgg aacgctacgt cgcattgtcg ccgctgaacg atcggtggta cgaggtgttc
     3481 atgcagggag agtctgatgg agtcgatgtc tacatgcggg atgtcactga ccttaaacga
     3541 tccgaggaaa tcctcagggt gagcgaggag aagtatcggg gcctgtttga aaatgtacag
     3601 gaatccgtcg caatctaccg gatggtatat gatgaaaacg ggaaggcggt tgatcggatc
     3661 tttgttgacg ccaatccaaa ggcattgagc cagatgggag atttaagacg ggaagatgtg
     3721 atcggaaagg catattctga agtcgtttca cgaaaatttc ccaaagacaa aatcagcatc
     3781 gacaaacatc tccagtccct tgcagcagtt gtacaatcag gaattccggt aacctatgac
     3841 acccactttg gcggcaaata ctatctgaca acacaatatt ctctccataa ggacctcgtt
     3901 gccagctcca gcattgatat taccgaacgt aagaaagccg aagaggctct ccgagagagc
     3961 gaggaaagat accgggtgct cgttgagaat gcgaacagca ttatactgaa aatggataaa
     4021 gaaggtaaaa tttccttctt caatgaatac gcccagaagt tctttggata ttcccttgac
     4081 gagatcatcg gacaggatgt gaaactactt gttccgccga ctgaccgcag cggtcgaagt
     4141 ccggaagaaa tgatagatgc cattctcaca gatccagatg ctttcgctga aaacgagaac
     4201 gaaaacatcc ggaagaatgg agaacgtgtc tggatttcgt ggaggaatag ggcgattcgg
     4261 gattctttgg gaaatctcat cgggaacctt gcaatcggac aggatattac cgaacgcaaa
     4321 caggcagagg aggcactccg gacaagtgaa cagcgtttcc ggctcgcgct gaaaaatgca
     4381 ccggtatctg tggcagtcca ggacactaac ctgatctatc tctgggcata taacgatggt
     4441 acaaaaaaat ctgaagatat tcagggcaag acggataccg atattttcac cccagaagat
     4501 gccgccatgc tgaccggact caagcggaag gttatcgaga ccggaaaaga agcccatgcg
     4561 cagatctggc tgaccgtgaa tgaaaaacgg gtatattatg atatgtacct ggaacctttg
     4621 cgaaacgaca ccggacagat caccggtatc ggcacagcaa ccgtagatca gaccttagag
     4681 aaactcactg atatcgcatt gcgggaaaac gagaaacggc tgagggaggc aaatgacctc
     4741 ctggaagcag ttaccgatgc tacaaaagtg attattgcag cggaagatac ggacttccgg
     4801 tacacgtatt tcaataagac gtatgcagca attatccgca ggcttaccgg taaagagatc
     4861 atgatcggta tgagcatgat cgacctgttc tctgacctgc ccggggagca gcggtctgaa
     4921 ctggacgagt ggggcaaggt gctcaacgga aagagcgtga acgaccacat cacatttgat
     4981 gacccggtta aggggcagac aatttataat gtgatccatg tccctctgcg gaatgcgaag
     5041 tgggagatca tcggggccgg ggaaattgcg ttcgatgtaa ccacacaggt ccaggtggaa
     5101 gagacactgc gggaggcaag ccagtacctc accaacctga ttgactacgc aaatgctccc
     5161 attatcgtct gggatcctga gttccgcatc accctgttca accatgcctt tgaacacctt
     5221 accggcagaa aagcaaagga agtcgtcggg caccatcttg aaatgctctt cccggaaaac
     5281 tatctcgccc cggccatgga ccttattaaa aaaaccagtg aaggggaacg atgggaaagt
     5341 gtggaaatac ctatcctcca aaagacagga gagatcagga ctgtactctg gaactctggc
     5401 actatttttg gatctgatgg gaaaaccatt attgcaacaa ttgcccaggg acaggatatc
     5461 acagaccgga aaaagataga atcagagtac agacacaggg ctgctgaata tg
//
LOCUS       DASRLV010000048        34427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1149907,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000048 DASRLV010000000
VERSION     DASRLV010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 34427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34427
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149907"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VFC43_01865"
     CDS             <1..656
                     /locus_tag="VFC43_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HZK29969.1"
                     /translation="LLDLDMHGPNIPKMLGIEDHKLAMIGERIEPVHITGKLSVISMA
                     FLLPDTSTPIIWRGPMKMAAIQQFLSEVNWGSLDYLVVDLPPGTGDEALTIAQLAPNL
                     RGAVIVTTPQDVATMDARKSVKFIQKLDVPVLGIIENMSGMICPHCKEQIDLFGKGGG
                     KKIADELAVPFLGSIPIDIDMRIAGDEGRPFIIRRGNSPTWEAVDKVMESLIKVIEG"
     gene            697..966
                     /locus_tag="VFC43_01870"
     CDS             697..966
                     /locus_tag="VFC43_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29970.1"
                     /translation="MPIYSGVIACLENPLAFPDMLEPIYREAMKLDDDTLDRFRFSLM
                     RLQLYADVHRNEDLEKAMHIKYVAQVLEKVIFGSLVMEREEPSAD"
     gene            1211..1666
                     /locus_tag="VFC43_01875"
     CDS             1211..1666
                     /locus_tag="VFC43_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29971.1"
                     /translation="MTSRMQSPHTTCPGCQEEVFLDELVGGKCPLCGCSLEEFDESYG
                     EYEGILDRSDLSWLIFNYFVFKKFVDLGVPPHQIMEFVAAYEQNAEKPPEEWTKTGFA
                     LEIPMNTWDHIKPKYCKKCKKLFFRGGKKSIHGDMRNTNLRVEYICDSC"
     gene            1925..2809
                     /locus_tag="VFC43_01880"
     CDS             1925..2809
                     /locus_tag="VFC43_01880"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZK29972.1"
                     /translation="MDAEEWVKSGNDLYLLGRYQEALDAFEGALSGSPDYAPAWNNKG
                     LALYVLGRYQEAADSYEKALKIDPGYVKAWNNKGLALDKLGRYQEAVDSYEKALKIDP
                     GYVKAWNNKGLALDKLGRYQEAVDSYERALSIDPNDATVWHNKGLALDKLGRYQEAVD
                     SYERVLSIDPNDATVWNDKGYALNKLGRYQEALDAYEKVLSREQNDKSLWYNKGLVLY
                     KLGRNQEAVKAYDEALSIDPDYVPALLNKGSALHKLGRYQEAVTAYDEVLSRDPDRVT
                     AWYNKGLALGELGRISKR"
     gene            complement(2889..4220)
                     /locus_tag="VFC43_01885"
     CDS             complement(2889..4220)
                     /locus_tag="VFC43_01885"
                     /inference="COORDINATES: protein motif:HMM:NF027569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcdB/YcdC domain-containing protein"
                     /protein_id="HZK29973.1"
                     /translation="MNKNPKDSGSIYLPERYRQQIEAKKKRRLVKKIALIGGIIAIVA
                     VVYLVVSGIPGFLNGSAPLLPVSTFLSPENVSKSATGEPGTLQSENISLLENPNLSIG
                     GGVPLLPTPDMVSLDNATRFLRLDYPAHAYSLISVNLTDQYSGHILYEFRIRPTGSGK
                     DDSGYPVFVDARTGDPYTPGQDSARITADRAKTLIKEAFPRLRPDTIRVRYDNRPESF
                     LAWIFSVTTGNMTVLTGTLDPETGQVLSFSLVTSWEDRQAEPLLDISAAQNIADRYIL
                     EKNRVPLPLNMSEARYIPLKVSQKTVAGHYVFVYNRIVQGIPCDSDGFTISIDSVSAE
                     VTGYERRWNSPDSAFSVALDPLVTRYEATFYILQRARETHPESVDSLSIVSAEIRWKD
                     HTVAGSFPRPGSIPMAWKVQFTDGIIGPKQVSALGWVDVQTGEILDFYYPH"
     gene            complement(4387..5823)
                     /gene="proS"
                     /locus_tag="VFC43_01890"
     CDS             complement(4387..5823)
                     /gene="proS"
                     /locus_tag="VFC43_01890"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HZK29974.1"
                     /translation="MDDDSGALPSKQDFSAWYNDLLWRAEIMDVRYPVKGLYVWFPYG
                     FALRKHVYQRLRDLLDRDHQETLFPLLIPEQEFMKEAEHIKGFEEEVYWVTHVGTTPL
                     DVKLALRPTSETAIYPMYALWVRSHADLPIKYYQIVNTFRYETKQTRPLIRLREITSF
                     KEAHTVHATWDEAEAQVEEAIRLYREFYDGLCIPTLVSKRPDWDKFPGADYTIAVDAI
                     MPNGKTLQIGTVHHLGDHFSRTFNITYEDKNGEQKFAQQTCYGISERSIAALISIHGD
                     DKGLILPPAVATLQAVIVPITIGKRHEDVMAAANKLYTDLTSAGFRVKLDSRDMRPGA
                     KYYWWELRGVPLRLELGPRDLDAGKVMAVTRTGQKLSIELVSIVDGITTVFREITDAI
                     RERAEKHTESHLCSVTSLADLTTTLDSGNVAVVTWCQQRECGDAIETKTNASILGTDV
                     RSCYVPASDGPCIECGKPGKATLVGRAY"
     gene            complement(5836..6402)
                     /locus_tag="VFC43_01895"
     CDS             complement(5836..6402)
                     /locus_tag="VFC43_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HZK29975.1"
                     /translation="MSDTRLQNAIIITFLLFFATITVGWIGTIQNPEIGENLMKLFQK
                     EVADQISGKEAPDIFIKLFLNNLEACILLFIGGASFGILTIFIMSLNGIVIGAIMEIV
                     RKDHTPAFVAAAILPHGIFEIPAFIISGALGIVLAQSLIAEWYNGADAAGDAQKFGRI
                     FLLYVLPLVVVAAFIEAFITPVIIHLVA"
     gene            complement(6395..7261)
                     /locus_tag="VFC43_01900"
     CDS             complement(6395..7261)
                     /locus_tag="VFC43_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF63 family protein"
                     /protein_id="HZK29976.1"
                     /translation="MISDFIYKYYIDPIRLGQSYNIVDTLTYAVILVIGVYLLYRWMS
                     QSTYLSDGGITIDAGFILATLPYVVLGGVLRVIQDTGMIKSDFQFLLVTPLIYFVLFF
                     FTIAMLFLSRSIALKGFTKNSLAFYAIAGVMSTFVVSLVLLAWGIRYAHFDLFILAVI
                     PLMALTATLLVWACMRYLLNWKYVTDPLYLTLIFGQLLDASATSYGIDLHPSIQYMEQ
                     HVVGSGLIELTHTAFVMFPLKLVVLFPAIYIMELYRKEANPAFWHLVLLAMIVVGLAP
                     GIRDMMRMVLYV"
     gene            7273..8352
                     /locus_tag="VFC43_01905"
     CDS             7273..8352
                     /locus_tag="VFC43_01905"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-1-phosphate
                     dehydrogenase"
                     /protein_id="HZK29977.1"
                     /translation="MSADAIKLLKPKVFDKSRWMQLPRDVVIGHDVFGQIPSVCEDLK
                     LGTSALLISGKGTMVLAGNNVLDILSKNYSVKPFIAEEISVPIIKKAQKAAAGMDFIV
                     GIGGGRVIDTAKIVSYNLDIQFISIPTAASHDGIASSRASVPTVEGHASLDAQPPIAI
                     IADTGVIASAPHRLLAAGCADIISNYTAILDWELAHRVKGEPMSEYAVALSKMTAEIL
                     VKNAHLIKPHQEQSAWFVTKALVSSGVAMSIAGSSRPASGGEHKFSHALDKLAPGKAL
                     HGESCGIGTIISMYLHGGDWRGIRASLKCIGAPVTPADIGVDDAIAVEALLMAKTIRP
                     ERFTIFDMGLTRESAQNLIQMLYSE"
     gene            8365..8811
                     /locus_tag="VFC43_01910"
     CDS             8365..8811
                     /locus_tag="VFC43_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0179 family protein"
                     /protein_id="HZK29978.1"
                     /translation="MAEVKTKVTLVGTVMAKQGVEFIYEGEVAGCDTCKVKKACNNLQ
                     PGRKYRIVSVRTTHHECGVHLNGTTAVEVIDAPVTALINADMAMVNSKIKLELSCSKN
                     ECRSYPLCRPDGVVTGEKYIVHTVLGKPSDVCEKGRALKLVEIKPA"
     gene            9015..9767
                     /locus_tag="VFC43_01915"
     CDS             9015..9767
                     /locus_tag="VFC43_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29979.1"
                     /translation="MKEDTRWKIFAGIGLVTLSLAMFTAHYLIFQDSHHLFIFFIGDI
                     AFIPIEVLVVTLIIDQMLESREKQRRLEKLNMVIGTFFSIVGTPLLVQLAKADTSLDS
                     VKHRLVIGTDWRKEMFGDVQSCLNAYTCNIGFSLVDSQTLREFLISREDFILRLVENP
                     MVFEHESFTDLILALNHLTEEMKARGDLSALPPADVSHLTTDITRVYSQLILEWVKYM
                     EYLKTHYPYLFSLAMRKNPFDPSASVVVTQDS"
     gene            complement(9908..10804)
                     /locus_tag="VFC43_01920"
     CDS             complement(9908..10804)
                     /locus_tag="VFC43_01920"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HZK29980.1"
                     /translation="MKFISGYLRACGSLIGLAIGDALGAPLEGSAQRESWVTEMLSGG
                     RHFRKKGSITDDTLQAMAVAESLASCKGFNQKDLISRLFSGYEKRPEWYGPTSSAFFA
                     LVKSGTLPHQAAWLVHTRLHGSRSNGSVMRGFPVGIFYPSPQVYDVSLACSQLTHFDP
                     VAAHCSAWLNTMVSDICRDVSRTKAYRHARQICTNPEVHTVLGNYTEYKPVASLDAVL
                     CSHAALFCFMNAHTFETAVLSAINLGGDTDTAGACCGAMAGAYWGLNAIPARWKRELE
                     DYEKILLCAEDLWNRREDSGKR"
     gene            10859..11125
                     /locus_tag="VFC43_01925"
     CDS             10859..11125
                     /locus_tag="VFC43_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0058 family protein"
                     /protein_id="HZK29981.1"
                     /translation="MQKEELLHLHMLMIHIKKYYEGVTSEEIGTERYNSLEISPVHIH
                     KDKKAHKDALLTLGDEIVTHIHGRQIPVVNFSSETASPKVAAGH"
     gene            11133..12710
                     /locus_tag="VFC43_01930"
     CDS             11133..12710
                     /locus_tag="VFC43_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HZK29982.1"
                     /translation="MDKELAIREMISRILTLQPGDDAIVAAKIDLCRKYGLDAVPKNS
                     AILAAAAPEDREILRKILLVKPTRTLSGVAPVAVMTSPAPCPHGKCLPCPGGPDHAFK
                     SPQSYTGEEPAALRAREHSYDPFAQVHARLEQFELLGHRVDKVELIVMGGTMTARTPE
                     YQEQFVSRCIEAMNVYPGGKPAKEPRDVDSVQEGNETSAIRCVAITFETRPDWCRQEH
                     IDRMLHLGVTKVELGVQHVDDTILSYNRRGCTVADTVNANTLLRDAGMKVGFHMMPNL
                     PGSTIDVDKKMFETLFSDSRFMPDFLKIYPTLVTPGSEIEELWKRGDYLPYNEEDMID
                     LVAYGKSLIPEYTRLQRIQRDIPAKLIVAGSRHSNFRQLAQNRLKVTGRRCRCIRCRE
                     IGRLPTLAESEIRVLQYACCGGQEHFISAVANDSLIGFTRLRFPSRVYRCELDNAALL
                     RELHVYGSPVPVGKDAGSEEWQHRNYGKVLISRAEEISRDSGFSRLAIMSGMGVRPYY
                     RRQGYERKGPYMIKELP"
     gene            12707..13837
                     /gene="priS"
                     /locus_tag="VFC43_01935"
     CDS             12707..13837
                     /gene="priS"
                     /locus_tag="VFC43_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase catalytic subunit PriS"
                     /protein_id="HZK29983.1"
                     /translation="MNPATTEFLRQRFTDYYKKTVLIAPSSLEQREWGFVLFSPQSTT
                     LRMRRHIGFSGKDELFQFIKNLVPQHAYYSTAYYEKPDAGTMAEKGWCGADLIFDLDA
                     DHIMRGAYDRMLARVKEETEKLLAMLIGELGMNPKTIELVFSGGRGYHVHVKDIAFRG
                     WGSAERRELIDYVCGIGIDPAAMLSLKKPVSPGWQVRYRETLAGYLSWLGTLDEEEAM
                     AHLVEIEGIGKDSAAGFLKNRNQVLSSLERSPSGMLVKNRVLHAILSQQEGEFKKRLL
                     SRAALADEPVTTDTKRLIRLPTSLHGGSGMRVQPLEIRDLHEFEPLVDAVVFSEREVR
                     VTLLHQVKIALLGSTYELQKGINTVPEAVAVFLCCRGMAEIS"
     gene            13842..14525
                     /locus_tag="VFC43_01940"
     CDS             13842..14525
                     /locus_tag="VFC43_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK29984.1"
                     /translation="MKIIGPLGLDELRSILLSERETGRLTAVVPDLFDRGYEELASLT
                     RKVYAFEDPLSDDARSLIEETLAIKETLRELFAIRSRKILALTIMHAEGNYYDREEVK
                     RMVPGEKEMFDTVTASIEQCKGILLHTLPQHEKGKISEETVAQSIPEPVGDEEAGDAT
                     VLYSPPEPNAPLMPPCILVRVLEDMESFMGVDGRIYTLLKGDIVTLPERNAAVLSERN
                     IVLNMNLCK"
     gene            14564..14842
                     /locus_tag="VFC43_01945"
     CDS             14564..14842
                     /locus_tag="VFC43_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L44e"
                     /protein_id="HZK29985.1"
                     /translation="MKMPAKFNTYCPHCRNHQMHEVEKVKKGKTTGLHWIDRQKARRG
                     KVGNMGKFSKVPGGDKPTKKINVRYRCVTCGKAHLRKGYRVAKFELTE"
     gene            14850..15038
                     /locus_tag="VFC43_01950"
     CDS             14850..15038
                     /locus_tag="VFC43_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27e"
                     /protein_id="HZK29986.1"
                     /translation="MVREIRENRSKFFKVKCPDCENEQTVFEKASTVVKCVVCGHDLA
                     LPTGGKASIKAEILSELK"
     gene            15047..15835
                     /locus_tag="VFC43_01955"
     CDS             15047..15835
                     /locus_tag="VFC43_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     alpha"
                     /protein_id="HZK29987.1"
                     /translation="MLDREWPQESELVVCTVQNVKDFVAFVSLDEYGGRQGLIPISEI
                     ATGWIKYIRDHIREGQKIVCKVLNVDMTRGHIDLSLKDVNEHQRREKIREWKNESKAQ
                     KWLGFVAEQTGEPVLTLENTIFEKYGAFYPVFEDLVTDSETTLKKLELSKKVADTLLQ
                     VAHENVKVPKVEVTGHLYLTSTLPDGISVIKKALKSAEPKIAGVDIELLYLGAPTYRI
                     KVTAPDYKKAEKAIEKAANAAIAVMEKSGGEGKLVKKPKSGKNQ"
     gene            15832..15987
                     /locus_tag="VFC43_01960"
     CDS             15832..15987
                     /locus_tag="VFC43_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-protein complex protein Nop10"
                     /protein_id="HZK29988.1"
                     /translation="MSGHIRYCSNDLIYTLSAVCPVCGKPTASAHPARFSPEDKYGKY
                     RRMAKND"
     gene            15980..16738
                     /locus_tag="VFC43_01965"
     CDS             15980..16738
                     /locus_tag="VFC43_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome assembly chaperone family protein"
                     /protein_id="HZK29989.1"
                     /translation="MIEEISVRYLDTSAEKKPADPILIEGLPGIGQVGKLVAEYMIHM
                     LGAEKIGEIHSIYFPPQVILEENGMARLARNELYCYRSLGKDLVFLVGDHQSTSNEGH
                     YLLADQYCSVAEELKVKRIYTLGGFGVGHLVNDPRVLGAVNRVELLPELKAAGVTFNR
                     EEPGGGIIGAAGLMLGLAAQRGIDAVCLMGETSGYLVDPMSAANVLTILSKLIDVPVD
                     PTKLNDRASEMEKFIEKFVEGNRLQKDEELSYIG"
     gene            16741..17862
                     /locus_tag="VFC43_01970"
     CDS             16741..17862
                     /locus_tag="VFC43_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HZK29990.1"
                     /translation="MLANADLHIHSPFSIAVSRFMQPEQLLAGCVTKGMDVLGTGDAL
                     QPVWQTGWEPYLENDAGIIIIPQGEIEDQNRVHHLILAEDFAQFSQLRDLLAGKCKSF
                     TTSGRPHVYLGGEEIAALAHEVGALIGPAHAFTPWTALYAYFGRVSDCYGSEPIDFLE
                     LGLSADSSYGAAIPELYTIPFLTNSDAHSPYPDKLGREFNRIRLNHRSVKDVLAAIKE
                     GAIAMNAGFFPEEGKYNRTACTRCYTQYSLTEATRYNWRCPGDGGIIKKGVSDRIKEL
                     SDGSEARPRPPYTRVIPLAKIIQTMENASSPNTKKCRAIYSSFISTFGNEITVLIDTP
                     VPEIRTIHEKVADAITALRTGTVRLIPGGGGKYGTFSLV"
     gene            17943..18119
                     /locus_tag="VFC43_01975"
     CDS             17943..18119
                     /locus_tag="VFC43_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZK29991.1"
                     /translation="MLKVHRDICGYCGCCVSVCPEGALELIDAYLCVEKSCTSCGICA
                     KVCPLGALEVIDEK"
     gene            18109..19305
                     /locus_tag="VFC43_01980"
     CDS             18109..19305
                     /locus_tag="VFC43_01980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZK29992.1"
                     /translation="MKNKYDVLVIGGGPAGAIAAKTAVEKGLSACIVEKRPAIGAPVR
                     CAEGIGKEALHEFIDPDPCWISAEMTGASIVAPDGFLMKLESQLAGNKVGYILDRKIF
                     DRELVWKAAEAGADIAVKTRASAPIMENGFVKGAKLESCGQVMSVTAEVVIAADGVES
                     KFSRWCGIDTTVPVREIMSSVQYVMADIDIDAHSTVFYLGNEVAPEGYLWVFPKGERS
                     ANVGIGISGKKSGQGHRARDYLDRFVKKTFPKGKPIEYIAGGVSVCRPLACTVADGLI
                     IAGDAARVVDPLTGGGIYNGMYTGRLAAEVAAECIAKGNVSKKALMVYDRKWREAKMG
                     KSIERNYHIKEYLIKLPDTKLNEIIHSVSKINLTEFSTLSLIKEIIRVNPKLMLELGV
                     LAASIR"
     gene            complement(19294..19962)
                     /locus_tag="VFC43_01985"
     CDS             complement(19294..19962)
                     /locus_tag="VFC43_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HZK29993.1"
                     /translation="MYIIIVGLGGIGKNLAKQAVEHANNVVVIDQDEARCSDVLEHYD
                     VLAVTGNATDKTTLEEAGIDRADALIATTSDDSVNLMTCWLAKKFHVPNVVSIVNQSS
                     HSDFFKEVGVRISENPDELVASRLYYWTQNPQLQQLASIPGGTIFEIVAEQGAPIVGH
                     EIRELKVKDFVFIAIRRSGGELIIPSGNVKIQPNDIFTVFTKKEAESDCLRLLNKQLK
                     KSAD"
     gene            20017..20658
                     /gene="rnhB"
                     /locus_tag="VFC43_01990"
     CDS             20017..20658
                     /gene="rnhB"
                     /locus_tag="VFC43_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HZK29994.1"
                     /translation="MICGVDEAGKGSVLGPMVVAAVGIPSEDVFSDLEVQDSKLLSPK
                     MRERLYTIIRKRFRVATVRIDAHEIDEIRTGITMNVCVARAHAQVIDRLSPSVAYVDA
                     CDVNAFRYADMVKSFLDQSCEIVSEHHADQTFRVVSAASIVAKVTRDRAISTLAKKYG
                     NIGSGYPSDPVTIAFLSEYFDEHKCPPPIARKSWKTVTTMIAQKQQSRLSEFF"
     gene            20736..22028
                     /locus_tag="VFC43_01995"
     CDS             20736..22028
                     /locus_tag="VFC43_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZK29995.1"
                     /translation="MNWILPVILLIAVYAAIAYYIHSRKLWEDRITFYGPIMAIKTNR
                     IGFFDKFIAFRTFFRIYGTAGVAAVIIVSVFITLMLFISVRYTLLIQPEPTGIYKPQN
                     ILLLPGINEYVPSTLAVWLAFIITIAVHEFGHAILCRVENIKVKTMGVLVAVIPIGFF
                     VEPDEEELDRTRGMAKIRMFGAGITNNLVVGFSCFILLILCMGLVVPVTQPIIHGVYK
                     DYPAAIAGISPGSIVTAVNGVSVTNRADVSTILNTTKPGTILTLTVEKDSVSRDYPLT
                     LSAWPGDIPDRTSGFMGVEYYDGSAVMGVIQGMLSPIGFFQFLIVPFASDSGVQFLRI
                     LAFETPDMTYYQVPFEGFWGVIHLLFWCAWINLNVGIFNAIPMIPLDGGYIFKEGVDR
                     LLDRRGLMKYSGYVTGAVSYLMLVVLISLIALPYLLHI"
     gene            complement(22093..23133)
                     /locus_tag="VFC43_02000"
     CDS             complement(22093..23133)
                     /locus_tag="VFC43_02000"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HZK29996.1"
                     /translation="MTIPLKKIAVMTGGGDCPGLNAVIRSVVLAAAGHGIKTLGIHNG
                     WQGLIEGDLEVLTTASVSGILSHGGTILGTSRTDPLKNPDDIGKIQKTMKTSGIDALV
                     VIGGDGTLSAASDIAGAGIHLAGIPKTIDNDISGTDSSVGFDTAVSIVTEMIDRLHTT
                     AESHHRIMVVEVMGRNAGWIAVMAGIAGGADMVLIPEERFTMDEVCRELKARYMAGKK
                     YSIVVVAEGIHHEDISAAKVPECDRDECGHEKFVGAGNLLGKEIENRLGIETRVTVLG
                     HVQRGGTPTAFDRILGTRFGAEAVEQLAAGNTGFMVALQGSRIGTIPLTEVASRIKPV
                     SPVFRHLVKTQK"
     gene            complement(23144..24109)
                     /locus_tag="VFC43_02005"
     CDS             complement(23144..24109)
                     /locus_tag="VFC43_02005"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HZK29997.1"
                     /translation="MNYLNFKETIAASPYIFTLGVAGDSGTGKTTFTEGIRSVFGTDL
                     VSTITLDDYHRLDREGRKEQGLTALNPEANRIDQLEHDLILLKRGVPIEKPVYNHSTG
                     VFDPPVIFCPQKILILEGLHTLFTPTLRKYLDFTLFVDPSKAVKSDWKIRRDMEKRGY
                     SRDAVLLEMAEREPEYERFIAPQKMFADAVIGIDYSEYGKDLGKERNVYRVTLSQNRM
                     IQSIENIDLSLDLYSILSLSERNFSLKFLTNEQNGQKIGEMIIDGELSEHVVKKLELS
                     IEQQTRVHPISIFKNRTYITAGELAQLILCWRIIHRRIFIEKNPQ"
     gene            complement(24206..25306)
                     /locus_tag="VFC43_02010"
     CDS             complement(24206..25306)
                     /locus_tag="VFC43_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HZK29998.1"
                     /translation="MAVFETLIVIFLILVIVAIFAKGVVIVQPYEQGLQIRLGQYVGR
                     MNPGFRWIVPFVSEAIKLDLRTTVIEIPSQEVITKDNSPTNVDAIIYIRVIDPEKAFF
                     EVGNYRLATVALAQTSLRGIIGDMELDEVLYNRDVINTKLRDILDRETDQWGVKVERV
                     EIKEVDPIGAVKNAMTEQTAAERARRAAILRADGEKRSAILKAEGLRQSMILEAEGER
                     QSKVLRAEGERLSRILQAQGEAQGLRILSVGAAPLDKKAITVLSLDALKIMANGQATK
                     IIFPFEISQLIRQGAKFLGAADETPEEAAERPVMDESILGDIPTREAVDAILKDIQDA
                     VPHVSEDELKDTSKRRLPIEKEKWESTLKPAE"
     gene            complement(25308..25766)
                     /locus_tag="VFC43_02015"
     CDS             complement(25308..25766)
                     /locus_tag="VFC43_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HZK29999.1"
                     /translation="MVLLLGIDLGWLLIMSGALLLLVEVYSPGFFATVPATVMIFLGI
                     LFLLGVNVFNSGWGVVAGVIIAIGAAGFTVWMYGKITPNESPTTISRDSLVGMEGQVK
                     IRVDATTISGKVLIASTEWSARSTGCEIPPGKKVKVVDSQGVHVVVEEVE"
     gene            complement(25832..26689)
                     /locus_tag="VFC43_02020"
     CDS             complement(25832..26689)
                     /locus_tag="VFC43_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HZK30000.1"
                     /translation="MKITEHCIDCLLSRVNLECSLCRASPSLADTTMAECSRIMNEIR
                     SAPLSHPQIASRIHRRAYEVLGNSDPFAELKASGNQQAQEVCKKICSELSTFRDFALA
                     SVIGNTFDYGVKGHIVEENFYDFFKKEFDRGFFIDDTDKIAPLCDRVVYLSDNCGEIV
                     LDRLFIEYLRSQNSHITLAVKEAPILNDATLADAKELGLDTCVDILTTTGGGAEIGIC
                     MENIPDNLRKAISRCTIIIAKGMANFESLSEMSNLPPIAYLMAAKCKPVADEVGVPVG
                     SKIAMLRTG"
     gene            complement(26720..26793)
                     /locus_tag="VFC43_02025"
     tRNA            complement(26720..26793)
                     /locus_tag="VFC43_02025"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26756..26758),aa:Pro,seq:ggg)
     gene            27122..27757
                     /locus_tag="VFC43_02030"
     CDS             27122..27757
                     /locus_tag="VFC43_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZK30001.1"
                     /translation="MYTILVVDDSPFIVDVFVTMLERGGYRTVAAYGGEECLEILKTV
                     TPDLILLDIMMEPVDGWETLEKIKENPQTKEIPVLMLTAKQLSPAEAQEYGIYIEDYV
                     LKPITHRELYDAIEHVLHRRQAIKSDMDIARQSGFDQEIISEYARLSKTIDVNKRLMK
                     ILETTYNFDDSKVRVSEEISNAIRNMEINIKFQESRLQQIKGELTGTPETK"
     gene            27977..28050
                     /locus_tag="VFC43_02035"
     tRNA            27977..28050
                     /locus_tag="VFC43_02035"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28011..28013,aa:Lys,seq:ttt)
     gene            28127..28378
                     /locus_tag="VFC43_02040"
     CDS             28127..28378
                     /locus_tag="VFC43_02040"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit H"
                     /protein_id="HZK30002.1"
                     /translation="MSTKLNVLKHVMVPDHKIMSEEEVSALLSHYNITSEQLPKIFHD
                     DPAVKTIGANVDDVIRIIRMSHTAGRAEAYRLVVKRPKK"
     gene            28387..29955
                     /locus_tag="VFC43_02045"
     CDS             28387..29955
                     /locus_tag="VFC43_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B''"
                     /protein_id="HZK30003.1"
                     /translation="MVSLIDRSILSKAYFSREHVARHQLDSFNYFLKHNLQKVVNEQK
                     VIETDIENRGKNNEPVWVELGTITVKKPLVREADGSQGELYPCEARLRNLTYAAPIQL
                     GLTLVHGNERQEPIITTIGQLPIMVGSQSCNLFGMDDAQRVSHGEDAFDPGGYFIVNG
                     TERVLMTLEDLASNKIMTEFTERYNERIYVAKVFSQFRGYRALVVVERNKKNLLEVSF
                     PSVAGHLKFVDLMRALGMTNDQEVVNAVSPDEDILTFMMQNLEESECDSVEGGIMYVG
                     KKLAPNQTKDYQRKRAEFVLDNYLLPHLNYSMPANLKEGDEGYQEAVLSVRLAKGHFL
                     GRMAEACFDLVLDKRRVDDKDHYSNKRLKLAGDLMEDLFRISLNRLTRDIKYQLERAS
                     MRHRDLSIATAVRADVLTERLLHPLATGNWVGGRTGVSQLLDRVDHMAVLSHLRRVIS
                     PLSRSQPHFEARDLHPTQWGRICPSETPEGPNCGLVKNFAQMVEISKGVDDEAEVMRL
                     LHSLGVEHIRGETR"
     gene            29952..31766
                     /gene="rpoB"
                     /locus_tag="VFC43_02050"
     CDS             29952..31766
                     /gene="rpoB"
                     /locus_tag="VFC43_02050"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B"
                     /protein_id="HZK30004.1"
                     /translation="MKKSRVFVDGALIGLEDDAKALVASIRSMRRQGAISSELNVSHK
                     EYNGDVVILTDRGRARRPLIVLKNGEPLITSEDLAKLARKEIDVSSFIQRGLIEFIDA
                     EEEEDLLIAMNPENVTPEHTHMEIDPSLILGIGAAHVPFPEHNASPRVTMGAGMVKQA
                     LGFGCANMKLRPDTRGHLLHYVQKPLTHTQTSELIGSDDRPAGQNFVVAILSYEGYNI
                     EDALIFNKASIERGLGRSHFFRTYEGEERRYPGGQIDRIEVPEEDVAGAHGADSYKNL
                     DEDGVINPETVVAEKDVLIGKTSPPRFLEEPSGELIAVEKRRDTSVTMRSNEHGIVDT
                     VIITEGENSSRLVKVRTRDLRIPEVGDKFASRHGQKGVVGLITHQEDMPFTESGLCPD
                     LVINPHAIPSRMTVGHMLEMIGGKVGALEGRRINGTAFRGESEANIRASLKNLGFSHT
                     GREIMYDGITGKQFKADVYVGVIYYQKLYHMVSSKMHARSRGPVQVLTRQPTEGRARE
                     GGLRFGEMERDVMIGHGAAMALKERLLDESDKVQEYVCAHCGMVAMLDRKRNMTRCLA
                     CGNETDIYSVEMSYAFKLLLDEMKSMGIAPRLRLQDVV"
     gene            31782..34421
                     /locus_tag="VFC43_02055"
     CDS             31782..34421
                     /locus_tag="VFC43_02055"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A'"
                     /protein_id="HZK30005.1"
                     /translation="MPSPKRIGQIEFGLLSPKEIREMSVRKIIWADTYDDDGFPYPQG
                     LMDLNLGVIDPGLRCKTCDQKASECPGHFGHIELAKPVIHVGYTRLIRKLLRVTYRSC
                     GRVLLSPDEIEKVVGTEDDQTGDLLSEKDVKKERICPHCGDQQLKINFEKPTTFSEVM
                     VEDGKKVEHKLTPADIRARMEKIPNEDLTPLGINPDVARPEWTILTVLPVPPVTMRPS
                     IILENGQRSEDDLTHKLVDIIRINQRFKENQDAGAPQLIIEDLWELLQYHVTTYLDNE
                     VAGCPPARHRSGRPLKTLSQRLKGKDGRFRGSLSGKRVNFSSRTVISPDPNLSVSQVG
                     VPRAVANEMTIPVRVTTHNIEELKQIVRNGPLRPDVNAPCGANYVIRPDNRRLRLADG
                     NLDTVADLIEPGYTVERQLRDGDVVLFNRQPSLHRMSIMAHRVKVMEGRTFRLNPAVC
                     PPYNADFDGDEMNMHVPQTEEARAEATILLSVHENILSPRFGGPIIGGIHDHVSGIFM
                     LTHGMRWFTKEEALYLLRYARVEHMPKPGKEENSVPYWSNKQIFSMILPDQLNMVFHA
                     SSCQNCDTCKKEMCERDAYVRIIDGILEAGTIDKKAIGAFDGQIVNRIIRHYSMRRAA
                     DFIDDLTKLSIRAIMFDGFSFGIDDEDLSKTEYAQIDEVLTAAASDVTRRIKIYEDGQ
                     LEPMPGRTVEETLEMQIMQVLGKARDQTGQIAGRHLGLGNSAVVMAVSGARGSMLNLT
                     QMAGCIGQQSVRGERIVRGYDDRTLPHFKKGDRGSDAHGFIAHSYKGGLNPTEFFFHA
                     IGGREGLVDTAVRTSQSGYLQRRMINALQDLKVAYDGTVRSTGGRIIQFQYGEDSTDP
                     TKSAFGDPVDVKGIVESILKEEV"
BASE COUNT         8824 a         8489 c         8770 g         8344 t
ORIGIN      
        1 gacttttaga cctcgacatg catggcccca acattcccaa gatgctggga atcgaagacc
       61 ataaactcgc catgatagga gaaaggatcg aacctgtcca tatcaccgga aaactttccg
      121 tcatatccat ggcattcctc ctgcctgaca cgagcacccc gattatctgg cgtggtccga
      181 tgaagatggc tgcaatccag caattcctgt cagaagtaaa ctggggctcc ctggactatc
      241 ttgtggtaga tctgcccccg ggaaccggag atgaggccct gacaattgca cagctcgccc
      301 ccaatctccg cggtgcagtt attgtcacga ccccccagga tgtggcaacc atggatgccc
      361 ggaaatccgt aaaattcatt cagaaactcg acgtccccgt gctgggaatt atcgagaaca
      421 tgagcggtat gatctgtcct cactgcaaag agcagattga cctttttggc aagggtggcg
      481 ggaaaaagat cgccgatgaa ctggctgtcc cgttccttgg ttcaatcccg atcgatatcg
      541 atatgcgcat agcaggagac gagggtcggc cgttcatcat acgccgcgga aacagcccta
      601 cctgggaggc tgttgacaag gtaatggaat cactcattaa ggttatcgag ggataatatg
      661 gattacctgc ggattctttc aggaaaggag gcacagatgc ctatttattc aggagtcatc
      721 gcatgccttg agaatccgct tgcatttccc gacatgctcg aaccaatata ccgcgaggca
      781 atgaaacttg acgatgacac gcttgaccgg ttccggttct cccttatgcg tctccagctc
      841 tatgcagatg tccaccggaa tgaagacctg gaaaaggcaa tgcacatcaa atacgttgcg
      901 caggtgcttg aaaaagtaat ctttggttcg cttgtcatgg aacgcgaaga accatccgcg
      961 gactaaaaag gtactccaac agcaaacctt ttatcgggag atacatgcac tggtgatacc
     1021 tatatgtgca gtgcagataa tacgattatg cagaattctg cggaactcat ggaaatgtac
     1081 agatgcccac agatgaggga taacctgtac gtggagaacc cggctaatac ccccacgaat
     1141 agtactgagt attagtttca gtccaacaca gatgaacatc aatactcttt gcttccttca
     1201 ggagactaca atgacatcac gcatgcaatc ccctcacacg acttgtccgg ggtgccagga
     1261 agaggttttt ctcgacgagc tagtgggcgg aaaatgcccg ctgtgcggct gctcacttga
     1321 ggaatttgat gagtcgtacg gggagtatga aggaatcctc gatcgttcgg acctctcgtg
     1381 gttgatcttc aattattttg tcttcaagaa atttgtcgat ctgggcgtgc ccccccacca
     1441 gatcatggag tttgtcgcag cctatgagca gaatgcagag aaacctcctg aagagtggac
     1501 caaaaccggc tttgctctcg agatcccgat gaatacctgg gatcacataa aacccaaata
     1561 ctgtaagaaa tgcaaaaaat tatttttccg gggcggaaaa aaatcaatcc acggggatat
     1621 gcggaatact aatctccggg ttgagtatat atgtgacagt tgctgataca tcaaaaaacc
     1681 ttttttttaa cgtgcaaatt gtcgagcaat ataaaaagaa ttctgatttg atttggaatt
     1741 ttgttttgac gcgtttttta ataaaaaact gtacggataa aaaaatgcaa gaagccctgc
     1801 tttttcactt cacctggata tcgttggacg atatgggtaa gatattaatt aaatttttca
     1861 atatccttat tttgttatac tccggtatgg tgaatagagc tggaattgcg ggggacatgt
     1921 gctgatggat gctgaagaat gggttaaatc gggaaatgat ctctaccttc ttggcaggta
     1981 tcaggaagca ttggacgcct ttgagggagc tttgtcaggt tccccggatt atgcgcccgc
     2041 atggaacaac aaaggactag cgctctacgt tcttggcagg taccaggaag cagcagattc
     2101 ctatgagaaa gcattgaaaa ttgaccctgg ttatgtgaaa gcctggaaca acaaaggact
     2161 ggcacttgac aaactgggaa ggtaccagga agccgttgat tcctatgaga aagcattgaa
     2221 aattgaccct ggctatgtga aagcatggaa caacaaagga ctggcacttg acaaactggg
     2281 caggtatcag gaggcagtgg attcctacga gagagcatta tcaatagacc cgaatgatgc
     2341 caccgtatgg cacaataaag gactggcctt ggacaaactg ggcaggtatc aggaggcagt
     2401 ggattcctac gagagagtat tatcaataga cccgaatgat gccacggtat ggaatgataa
     2461 aggatatgct ctgaacaaac tgggcaggta ccaggaagca ctcgatgcct acgagaaagt
     2521 cttgtcccgt gaacagaatg ataaatcgtt atggtacaat aaagggctcg tactttacaa
     2581 actaggcagg aatcaggaag cggtgaaggc ctatgatgaa gcattatcaa ttgacccgga
     2641 ttatgtaccg gcattgctca ataaaggaag tgcacttcac aaattaggca ggtatcagga
     2701 agcggtgacg gcctatgatg aagtgttgtc gcgtgatcca gaccgtgtga cggcatggta
     2761 caataaagga ctcgcacttg gtgagcttgg caggattagt aaacgttagg tgccggtagt
     2821 gaagcgtggt aattgacccg gattatggca tcgcacatga aaggcagact ttttgggata
     2881 ttaaaaaatt agtgagggta atagaaatcc aggatctctc cagtctgtac atcaacccag
     2941 cccagtgcgg aaacctgttt tggaccgata atcccatcag tgaactgcac cttccaggcc
     3001 ataggaatac tgcccgggcg ggggaaagat cctgccacgg tatgatcttt ccatcggatt
     3061 tctgccgaaa caatggaaag actatccact gattcaggat gggtttctct ggctctttgc
     3121 aggatataaa atgtggcctc gtaacgggtt accagagggt ctagggccac cgaaaaagcg
     3181 ctgtcgggag aattccagcg tcgttcatat cccgtgacct ctgcgctcac tgaatcaatg
     3241 gaaatggtga aaccgtcact atcgcaggga attccctgga cgatccggtt atatacaaaa
     3301 acatagtgcc cggcaacagt cttttgggag acctttaaag gaatatatct tgcctcactc
     3361 atgttcaggg gaagcggcac cctgtttttt tcgagaatat accggtctgc aatgttctgg
     3421 gctgcgctga tatcaagcaa tggttctgcc tgcctgtcct cccaggacgt aacgagagag
     3481 aaggataata cctggccggt ctcagggtcg agggttccgg taaggacagt catattaccg
     3541 gtggtcacgg aaaagatcca ggccagaaaa gattcaggac gattatcgta cctcaccctg
     3601 atcgtatccg gacgaagccg gggaaaagct tcttttataa gggtttttgc ccgatcggca
     3661 gtgatcctgg cactgtcctg tccgggggta tagggatccc cggttctggc gtcgacaaat
     3721 acgggatatc cggaatcatc cttcccggaa ccggttggtc tgatccggaa ctcatacaga
     3781 atatggccac tgtactggtc cgttagattc acactgatga gcgaatatgc atgtgccggg
     3841 tagtccaggc ggaggaacct ggttgcatta tccagcgata ccatatccgg ggtcggcagt
     3901 aaaggaactc ctcctccaat gctgaggttg gggttttcta atagggaaat gttttcggat
     3961 tgaagggtgc cgggttctcc tgttgctgat tttgacacat tttcaggaga tagaaacgtt
     4021 gatacgggaa gtaatggggc agacccgttt aaaaaaccgg gaattccgct tactaccaga
     4081 tacaccactg caacgatcgc tattatcccc cctatcagag caattttttt aacaagacga
     4141 cgtttcttct ttgcttcgat ctgctgcctg tatcgttcgg gcaggtatat ggatcctgag
     4201 tctttgggat ttttattcac tgctgccgcc tcaaatactt atctagtata gggtctttac
     4261 cagtaaaaaa ggaacgggct ctgcgcacga taaataagcc tcagggaaaa tctcgtgtgt
     4321 gatacaaggt tgcgtggggt taaaataaga aataaaggga aaacaacagc tctttttttc
     4381 atgcttttag taagctcttc cgacaagggt tgctttaccg ggtttcccgc attcaatgca
     4441 gggaccatca gaggccggaa catagcatga acgcacatca gttccaagga tactcgcatt
     4501 cgtcttggtc tcaatcgcat ccccgcattc ccgctgctgg caccaggtga ctactgcaac
     4561 attcccgctg tccagcgtag tagtaagatc tgccagggat gtcactgaac aaagatgaga
     4621 ttccgtgtgc ttttccgcac gttcgcggat agcgtccgtg atctcgcgaa agaccgtggt
     4681 gatcccatca acgatcgata cgagttctat cgacagtttc tgaccggtcc ttgtgacagc
     4741 catgactttt cctgcatcga gatctcttgg ccctaattcc agcctgagcg ggacaccccg
     4801 caactcccac cagtaatatt tcgctccggg acgcatatca cggctgtcaa gtttcacccg
     4861 gaatccggcg gaagtgagat cggtgtaaag tttatttgcg gcggccatga catcttcgtg
     4921 gcgtttcccg atcgtaatcg ggacaatcac tgcctggagt gttgcaactg caggcggaag
     4981 gatgagacct ttatcatccc cgtggatact gatcagggcc gcaatggagc gctctgaaat
     5041 accataacag gtctgttgag cgaatttctg ctctccgttt ttgtcttcgt aggtaatgtt
     5101 gaacgtccgt gaaaagtggt cgccaaggtg gtgcacggtg ccgatctgga gtgtcttgcc
     5161 gtttggcatg attgcgtcaa cagcgatcgt gtaatcagcc ccggggaact tgtcccagtc
     5221 cgggcgtttt gaaacaaggg tcgggatgca gagaccatcg tagaattcgc gatagagccg
     5281 gattgcttcc tcgacctgag cctcagcctc atcccacgtt gcatgaactg tatgggcttc
     5341 cttgaaggaa gtgatttccc tcaaccggat gagcgggcgg gtctgttttg tctcgtaccg
     5401 gaacgtgttt acaatctggt aatatttgat cggcaaatca gcgtgcgagc ggacccagag
     5461 agcgtacatg ggatagatgg cggtttcgct ggtcggccgg agcgccagct tcacgtcgag
     5521 cggtgttgtt cccacatgcg ttacccagta cacttcttct tcaaatcctt tgatatgctc
     5581 ggcttctttc atgaactcct gttcagggat cagaagcgga aaaagtgttt cctggtgatc
     5641 gcggtcaagc aggtcgcgta acctctggta tacatgcttt cgcagtgcaa agccatatgg
     5701 aaaccagaca tacaaccctt tgaccggata acggacatcc ataatctccg cccgccatag
     5761 cagatcatta taccatgcag aaaaatcctg ttttgacggc agggcgcccg aatcatcgtc
     5821 cataaatcag atcctctaag ctactagatg tatgattaca ggcgtaataa aagcctcgat
     5881 aaatgccgcg actacaacga gcggtagtac gtagagtaag aagatccgtc cgaatttctg
     5941 tgcatctcct gcagcgtcgg ctccattgta ccactctgca atgagtgact gggcgagcac
     6001 gatccccagt gcaccggaga tgatgaacgc aggaatctca aagataccgt ggggcagaat
     6061 cgcagcagcg acaaacgcgg gtgtgtggtc cttcctcacg atctccatga tcgcacctat
     6121 gacaatacca ttcaggctca tgatgaatat cgtgagaatg ccaaacgatg cgccccctat
     6181 aaagaggagg atgcacgcct caagattgtt caaaaagagt ttgatgaaaa tatccggagc
     6241 ttccttaccg gatatctggt cggcaacctc cttttggaac agtttcatca ggttttcgcc
     6301 tatctcaggg ttctgtatgg tcccgatcca gcccactgtt atggtagcaa aaaagaggag
     6361 gaatgtgata ataatcgcgt tttgcagcct ggtatcagac atacaacacc atccgcatca
     6421 tatcccgtat acctggtgca agtccgacaa ctatcatagc aagcaggaca aggtgccaga
     6481 acgcgggatt tgcctctttg cggtacaact ccatgatata aattgccggg aagagcacga
     6541 ccagtttcag agggaacatg acaaatgcgg tatgggtcag ctcaatgagt ccggaaccca
     6601 cgacgtgttg ttccatgtac tggatggacg ggtgcaggtc aatcccgtaa ctggtcgcac
     6661 ttgcatccag cagctgacca aaaatgaggg taaggtagag tggatcggtc acgtatttcc
     6721 agttaaggag gtatctcatg caggcccata ccagcagcgt ggcggtgagt gccataaggg
     6781 ggatgacagc gagtatgaag agatcgaagt gagcatacct gatgccccat gccagaagta
     6841 cgagtgagac gacaaaggtg gacattacac cggctattgc gtaaaatgcg agactgttct
     6901 ttgtaaatcc cttaagggct atagagcgtg aaagaaacag catcgcaatg gtaaaaaaaa
     6961 agagtacgaa atagataagc ggggtcacga gcagaaactg gaagtcactc ttgatcatcc
     7021 cggtgtcctg gataacccgc aacactcccc cgagaacaac atagggaagc gtggcaagga
     7081 taaatccggc atcaatagta atcccgccat ctgaaagata ggtggactgt gacatccagc
     7141 ggtacaataa atataccccg atgaccagga ttactgcgta tgtaagcgta tcaacgatat
     7201 tataggactg ccccagccgg atgggatcga tgtaatattt gtatatgaaa tcactaatca
     7261 tcactgatca caatgagcgc agatgcaata aaattactca aaccgaaagt ttttgacaag
     7321 tccagatgga tgcagctgcc acgtgatgtc gttatcggac atgatgtatt cggacagatc
     7381 ccctctgtct gtgaagatct caaactcggg acgtctgcgc tcctgatatc ggggaagggg
     7441 acgatggtac ttgccggaaa taacgtcctc gacatactca gtaagaacta ttcggtgaag
     7501 ccgtttattg cagaagagat cagcgttcct atcatcaaaa aggcccagaa agccgcggca
     7561 ggaatggact ttattgtcgg tatcggtggc ggacgtgtaa tcgataccgc gaagattgta
     7621 tcctataacc tcgacatcca gttcatttcc attccaacag cagcatctca tgatggcatt
     7681 gcctcctccc gcgcctcggt acctacagtt gaggggcatg catcgctgga tgcccagcct
     7741 cccattgcaa tcattgccga tacgggtgtc attgcctctg cgcctcaccg tctccttgct
     7801 gccgggtgtg ctgacataat ctccaattat actgcaatcc ttgactggga gctggcccac
     7861 cgggtaaaag gagagccgat gagcgagtat gctgtcgcgc tctcgaaaat gactgctgag
     7921 atcctggtaa agaatgccca cctgatcaag cctcaccagg aacagtcagc ctggtttgtc
     7981 acaaaagctc tcgtatccag cggagtagcg atgagcatag ccggctcgtc ccgcccggca
     8041 agcggaggtg aacataagtt ttcccatgca cttgacaagc ttgcccccgg taaggcactc
     8101 catggcgaga gctgtggtat cgggacaatt atttccatgt acctgcacgg gggggactgg
     8161 cggggaatcc gtgcctctct gaaatgtatc ggcgccccgg ttacccccgc ggacattggt
     8221 gttgacgatg caattgccgt cgaagcgctt ctcatggcaa aaactatccg tccggagcgc
     8281 ttcacaatat tcgatatggg attgacacgt gaatcggcac agaatcttat acagatgctg
     8341 tacagcgaat aaaggagtat tcagatggct gaggttaaaa caaaagtgac gctggtcgga
     8401 acggttatgg ctaaacaggg cgttgaattc atctacgaag gtgaagttgc cggctgcgat
     8461 acctgcaaag taaaaaaagc gtgcaataac cttcagccag gccggaaata ccgtattgta
     8521 tcagtgcgga caacgcatca cgaatgcggg gtccacctca atggtaccac tgccgttgaa
     8581 gtcatcgacg cccccgtcac tgcactcatc aatgccgata tggctatggt gaattccaag
     8641 atcaaactcg aactgtcctg ttccaaaaat gaatgccgga gttatccgct ctgtcgcccg
     8701 gatggcgttg ttaccgggga gaagtatatt gtgcatacgg tgctgggaaa accttcggat
     8761 gtctgcgaaa aagggcgggc cttgaagctg gtggaaataa aaccggcata aatattttcc
     8821 tggcaacaca aaaaaacccg ggttctctcc ttttatcagc gatacattat ccttaaattt
     8881 cggtcgtaag cctgcagact tgttccaatc cgggaaaaaa gcgtactaaa cagatgcgat
     8941 ttcgtgctga aaatgttctc cccgcctgcg agcgtaacgg aattttattc ataccaaaaa
     9001 ccccattgac gtgtatgaaa gaggatacac ggtggaaaat ctttgcaggg attggacttg
     9061 ttacgttgtc ccttgcgatg tttaccgccc actacctgat ttttcaggat tcgcatcacc
     9121 ttttcatatt ttttatcggt gatattgcct ttatcccgat cgaagtactg gtagtaaccc
     9181 ttattattga ccagatgctg gaatcgcgtg agaagcagcg gcggctggaa aagctgaata
     9241 tggtgattgg gacctttttt tcaattgtcg gaacaccgct tctcgtacaa cttgccaaag
     9301 cggatacgtc tctggacagt gttaaacatc gccttgttat agggactgac tggcgtaaag
     9361 agatgtttgg cgatgttcag tcctgcctga atgcctatac gtgcaatatc ggcttctccc
     9421 tggttgactc acaaaccctg cgtgagttcc tgatcagccg tgaagacttt atactgaggc
     9481 ttgttgaaaa tcccatggtc tttgagcatg aatcatttac cgatcttatc cttgcgctta
     9541 accatcttac agaagagatg aaggcacggg gcgacctctc cgccctcccg cctgcagatg
     9601 tcagtcatct cacaaccgat atcacccgcg tttattcaca gctgatcctt gaatgggtca
     9661 aatatatgga atatctcaaa actcattacc cgtacctttt cagtcttgcg atgcggaaaa
     9721 atccatttga tccgtctgcc tcggtggttg tgacacagga ttcataaatc tctctctttt
     9781 tttttaaaaa aagcaaaatt atccaaaaag gccattcagg ggaaccactt ccttgtcatc
     9841 catatcatat ccggaaaaac tcatgtattg aattgggcaa aatatgggga aaaacctggc
     9901 ttgcgaatta tcttttaccg gagtcttctc tacgattcca gagatcttct gcacagagca
     9961 gtattttttc atagtcttcc agttcccgtt tccaccgggc cggaattgcg ttgagccccc
    10021 agtatgcccc ggccattgcc ccgcagcagg cacctgcggt gtccgtatcc ccaccaagat
    10081 taattgccga taagactgca gtttcaaagg tgtgggcatt catgaagcag aaaagagctg
    10141 cgtgcgagca gagcacagcg tcaagcgagg caacaggttt gtactccgta tagtttccca
    10201 gtactgtatg cacttccgga tttgtgcaga tctgccgtgc atgccggtat gcctttgtgc
    10261 gggatacatc ccggcagata tcgcttacca tcgtgtttag ccatgccgaa caatgtgcag
    10321 caaccggatc aaaatgcgtt agctgagagc aggccaggct cacatcatag acttgtggtg
    10381 agggatagaa aatccctacc ggaaatcccc gcataacact gccattgcta cggctgccat
    10441 gcagtcgtgt atgaacgagc cacgcggcct ggtgcggcag ggtgccggat ttgaccagtg
    10501 cgaaaaatgc tgaggatgtc gggccgtacc attccggacg tttttcgtac ccggaaaata
    10561 accgtgatat aaggtctttt tggttgaaac ccttgcatga agcgagcgat tctgcaactg
    10621 ccatggcctg cagggtgtcg tcagtgatgg agcctttttt ccggaaatgg cgcccaccgg
    10681 aaagcatttc ggtcacccaa gactcgcgct gggcagatcc ttcaagtggc gcacccaaag
    10741 cgtccccaat tgccagccca ataagggatc cacaagcccg caggtatcca gatataaatt
    10801 tcaccaaaaa ggtatatgtg catcataaga aaaagaatca gttggagaag gtgatagtgt
    10861 gcaaaaggaa gaattgctac atttacatat gctgatgatt cacatcaaaa agtactatga
    10921 aggcgtcacc agtgaagaaa tcggaaccga acgttataat tccttagaaa tttcccctgt
    10981 ccatatccac aaggacaaaa aagcccataa agatgctctt ctcacgctgg gagacgaaat
    11041 tgtgacccat atacacgggc gtcagatccc tgtggtgaac ttttcttcag agactgcatc
    11101 accaaaagtt gcagccggtc attaaatctg tcatggataa ggagctggca atccgggaga
    11161 tgatctcccg tatccttacc cttcagcccg gtgacgatgc cattgttgca gcaaagatcg
    11221 atctttgccg gaaatacggg ctggatgcgg taccgaaaaa ttctgcaatt cttgctgccg
    11281 cagcgcctga ggaccgtgag atcctcagga agatcctgct ggtgaaaccc acgagaactc
    11341 tttcaggagt ggctccggtc gcggttatga cctcaccggc accctgtccg cacgggaaat
    11401 gtcttccctg ccccggggga cccgaccacg cgttcaaatc gccgcagagc tacacgggag
    11461 aggagccggc agcattacgg gcacgggagc actcgtacga cccgtttgca caggtgcatg
    11521 cacgtctcga acagttcgaa ctcctcgggc accgtgtgga caaggtcgag ctgatcgtca
    11581 tgggcggcac gatgaccgcc cggactcctg aataccagga gcagttcgta tcgcgctgca
    11641 ttgaagctat gaatgtgtat ccgggtggga aaccggcaaa agaaccccgt gatgtggatt
    11701 ctgtccagga aggtaacgaa acttctgcta tacggtgtgt ggctatcaca ttcgagaccc
    11761 gtccggactg gtgcagacag gagcatatcg accggatgct gcatctcggg gtaaccaaag
    11821 tagaacttgg agtgcagcac gtcgatgata ctattctgtc ctataaccgg cggggttgta
    11881 ccgtggcaga tacggtaaac gcaaacaccc tcctccgtga cgccggaatg aaagtgggtt
    11941 ttcatatgat gcccaatctt cccggctcga ccattgacgt ggataaaaaa atgtttgaaa
    12001 ccctcttttc cgattcccgt ttcatgccgg attttctcaa aatatatcct acccttgtca
    12061 caccgggttc agaaatcgaa gaactctgga agcggggaga ttaccttcct tataatgagg
    12121 aggatatgat cgatctcgtt gcatatggaa aatctcttat cccggagtac acccgcctcc
    12181 agcggatcca gcgtgatatc ccggcaaaac ttatcgttgc gggttcccgg cactccaatt
    12241 tccggcagct tgcccagaac cggctgaagg taaccggacg gcgttgccgc tgtatacggt
    12301 gccgtgagat cggaagactc cccacccttg cagaatcgga aatccgggtg ttacaatatg
    12361 cctgctgcgg cggacaggag catttcatct ctgcagtggc aaatgactca ttgatcggtt
    12421 ttacccggct ccgttttcct tcccgggtat atcggtgtga gcttgataat gctgcactcc
    12481 ttcgggaact gcatgtatac gggagtcctg tcccggtagg aaaagatgcc gggtctgaag
    12541 aatggcagca ccgtaattat ggaaaagtcc tcatttccag ggcagaagag attagccggg
    12601 attccggctt ctcccgtctt gctattatga gcggaatggg tgtccgcccc tattaccgga
    12661 gacagggcta tgaacgcaaa gggccttata tgatcaagga actgccatga acccggcgac
    12721 aacagaattc ctcagacagc gtttcacgga ttattacaaa aaaactgttc ttattgctcc
    12781 ctcatcgctc gagcagcggg aatgggggtt tgtactgttc agccctcagt caacaactct
    12841 gcggatgcgc cgccatatcg gattttcagg caaggatgaa ctcttccagt tcataaaaaa
    12901 tctcgtgccc cagcacgcct attattcaac ggcatattac gagaaaccgg atgccgggac
    12961 catggcagaa aagggctggt gcggggcgga tctgatcttc gaccttgatg cagaccatat
    13021 tatgcgtggt gcctatgacc ggatgctggc gagggtgaaa gaggaaacag agaagctcct
    13081 tgccatgctc attggagaac tggggatgaa tccaaagacc atcgagctgg tcttttccgg
    13141 cggcagggga taccatgtcc atgtcaagga catcgcgttc cgtggatggg gaagtgccga
    13201 acgccgggag ctcatagact atgtctgtgg aatcggcatt gatcctgcag caatgctctc
    13261 tttaaaaaaa cctgtatcac ccggctggca ggtacgatac cgggaaacac ttgccgggta
    13321 tctttcctgg ctgggaaccc tggatgagga agaagccatg gcgcatcttg tggaaattga
    13381 aggtatcggc aaggattcgg ctgccgggtt cctcaaaaac cgaaaccagg tgctttcttc
    13441 ccttgagcgc tccccctcgg gtatgctggt aaagaaccgg gtgctccacg ccatcctgtc
    13501 ccagcaggaa ggagaattta aaaaacgcct gctttcccgg gcagctcttg cggatgaacc
    13561 ggtcactacg gatacaaaac gactgatccg tctgccgaca tcgctgcatg gcgggagcgg
    13621 gatgcgggtg cagccccttg agatccgtga tcttcacgaa tttgaaccgc ttgtcgatgc
    13681 cgtggtgttc tcggagcgtg aagtccgggt gacccttttg caccaggtaa agatcgcatt
    13741 gctcggaagt acttatgaac tccaaaaagg tatcaataca gtaccggaag cagtagccgt
    13801 atttctctgc tgccgcggta tggctgaaat atcataaaaa ggtgaagatc atagggcccc
    13861 tgggacttga tgagctgcgc agcattctgc tttctgagag agagacggga cgtcttactg
    13921 cagttgtccc tgatctcttc gaccgggggt acgaagaact tgcgtccctt acccgtaaag
    13981 tctatgcatt tgaagatcct ctttccgacg atgcacgctc ccttattgag gaaacactcg
    14041 cgataaaaga gaccctgcgg gaactttttg ccatccgctc gcgcaagata ctcgcgctta
    14101 ccatcatgca cgctgaaggt aattattatg accgtgaaga agtcaaacgg atggtccccg
    14161 gcgaaaaaga gatgtttgac acggttactg ccagtattga gcagtgtaag ggcatcttac
    14221 ttcataccct gcctcagcat gaaaaaggta agatatcaga agaaacggtt gcccagagca
    14281 tacctgaacc tgtcggggat gaagaagcgg gtgatgcaac ggtgctgtat tctcctcctg
    14341 aaccaaacgc ccccctcatg cccccctgta tacttgtccg ggtacttgag gacatggaat
    14401 ccttcatggg tgtggatggg aggatttata ctctcttaaa aggagatata gtgacgcttc
    14461 ctgagcggaa tgccgcggtc ttaagtgaac gcaacatagt cttaaatatg aacctttgta
    14521 aataatattg gtattctaca tccaaattat cggtgaaaat tttatgaaaa tgccagcaaa
    14581 atttaatacc tattgtcctc actgcaggaa tcatcagatg cacgaggtcg aaaaggtcaa
    14641 gaaaggcaag acaaccggcc tccactggat tgaccgccag aaggctcgca gaggaaaagt
    14701 aggcaatatg ggaaagttct caaaagtgcc cggtggcgat aagcccacaa aaaagataaa
    14761 cgtgcggtac cgctgtgtta catgcggaaa ggcacacctg cgcaagggtt accgggtcgc
    14821 aaagtttgaa ctgacggagt gagcgcataa tggtacgcga aattcgggaa aaccgaagta
    14881 aatttttcaa agtaaagtgt ccggactgtg agaacgaaca gacggtgttt gaaaaggcaa
    14941 gcacggtagt gaaatgtgtt gtctgcgggc atgaccttgc actccccacc gggggaaaag
    15001 caagcatcaa ggctgagatc ctctcagagc tcaagtgata cttcccatgc tggacagaga
    15061 atggccacag gaatcggaac tggttgtctg caccgtacag aatgtcaagg acttcgtagc
    15121 gtttgtttcg ctggatgagt acggtggccg gcaggggctt atccccatct ctgaaattgc
    15181 aaccggctgg atcaagtaca tccgcgacca cattcgcgaa ggacagaaga ttgtctgtaa
    15241 agtcctcaac gtagacatga cccgcgggca cattgacctg tccttaaaag acgtcaacga
    15301 gcaccagcgc cgcgagaaga tccgcgaatg gaaaaacgag agcaaagccc agaagtggct
    15361 gggttttgtt gccgaacaga ctggtgaacc ggtactaacc ctcgagaata cgattttcga
    15421 aaaatacggg gctttttatc cggtttttga agatctcgtg actgacagtg agacaaccct
    15481 caaaaaactg gaactctcaa agaaagtagc cgacacgctt ctccaggtcg cccatgagaa
    15541 tgtgaaggtc cccaaagttg aagtgaccgg ccatctgtat ctcacctcca cactccccga
    15601 cgggatatca gtgatcaaaa aggcattgaa gagtgccgag cccaaaattg ctggggtgga
    15661 tattgaactc ctgtaccttg gcgcaccgac gtaccggatc aaagtgaccg cccccgatta
    15721 caagaaagcc gaaaaggcaa tcgagaaggc tgccaatgca gcaatcgcgg tgatggaaaa
    15781 gtctggtggc gagggaaaac tcgtcaaaaa accgaagtcc gggaagaatc aatgagcggt
    15841 catatccgtt actgttccaa cgacctgatc tatactcttt ctgcagtatg tccggtgtgc
    15901 ggcaaaccaa ctgccagcgc acatccggca cgtttttctc ctgaagacaa gtatggaaaa
    15961 taccggagga tggcgaaaaa tgattgagga gatctctgtt cggtatctcg acacctccgc
    16021 ggaaaaaaaa ccggcagacc ccatcctgat tgaagggctt ccgggaatcg gtcaggtagg
    16081 caaactcgtg gcggaatata tgatccacat gctgggtgca gagaagattg gcgagattca
    16141 ttccatctat tttcctcccc aggtaattct tgaagaaaat ggtatggccc gccttgcccg
    16201 taacgaactc tattgttacc gttctttggg aaaagacctg gtctttctcg ttggggatca
    16261 ccagagtact tcgaatgaag ggcattacct ccttgctgac cagtactgtt ctgttgctga
    16321 agagctgaag gtaaagcgga tttatacgct cggtggattc ggtgtagggc acctggtaaa
    16381 cgacccccgg gtgctgggtg cagtgaatcg tgtggaactc ctgccggaac taaaagccgc
    16441 gggtgttacg tttaaccggg aggagcccgg cggcggaatt atcggtgctg cagggctcat
    16501 gctgggtctt gccgcacaac gcggcattga tgcggtttgc ctgatggggg aaacaagcgg
    16561 gtacctcgtc gatccgatga gtgcggcaaa tgtactcact atcctttcaa aactgattga
    16621 tgtgccggta gatcctacta aactcaacga tcgtgcttca gaaatggaaa agttcatcga
    16681 aaagtttgta gaaggcaacc ggttgcagaa agatgaagag ctgagttata tcgggtaatc
    16741 atgctcgcga atgcagacct gcacatccac tccccgttct cgatagccgt ttcacgcttc
    16801 atgcaacctg agcagctcct cgccggatgt gtgacgaaag gaatggacgt gctgggtacc
    16861 ggagatgctc tccagccggt gtggcagacc gggtgggaac cgtacctgga gaatgatgcg
    16921 ggaattatta tcattcctca gggagagata gaagatcaga accgggtcca ccacctgatc
    16981 ctggcagaag acttcgccca gttcagccag ctccgcgatc tgctggcggg aaagtgtaaa
    17041 agttttacaa caagcggccg cccccatgtc tacctgggtg gagaagaaat tgcagccctt
    17101 gcccatgaag tcggcgccct gatcggtcct gcccatgctt tcaccccatg gactgcactg
    17161 tatgcgtact tcgggcgggt atctgactgc tatggcagcg aacctataga tttccttgaa
    17221 ctgggtctct ctgcagacag ttcgtacggt gcggcgatcc ccgagctgta cacaattccg
    17281 tttcttacca actccgatgc tcacagtccc tatccggata aacttggacg tgaattcaac
    17341 cgtatccggc tcaatcaccg gtctgtaaaa gatgtcctcg ccgccataaa ggagggtgca
    17401 atcgcgatga atgcagggtt cttccccgag gagggcaagt ataaccgcac agcctgcaca
    17461 aggtgttaca cgcagtattc tcttaccgag gcaacccggt acaactggcg ttgcccgggc
    17521 gatggcggga ttatcaaaaa gggcgtttct gaccggataa aggagctctc tgatggcagc
    17581 gaggcacggc cgcgaccacc atatactcgt gtcatccctc ttgcgaagat cattcagacg
    17641 atggaaaatg catcatcccc caacacaaaa aaatgcaggg cgatctactc atcgttcatc
    17701 agcacattcg ggaatgaaat taccgtcctg atcgatacac ccgttccaga aatccgcaca
    17761 attcatgaga aagtggcaga tgcgatcaca gccctgcgga ccgggaccgt aaggctcatt
    17821 cccggcggcg ggggaaaata cggtacgttc tcgctcgtct gaagtccttt tagataaaac
    17881 agtgattcgg ccggcatttt atcgtaaata ttaatgaaac aggacaccaa cattatcact
    17941 caatgctcaa agtacatcgg gatatttgtg gatattgcgg ttgttgtgta tcggtctgcc
    18001 cggaaggtgc gcttgaactg attgatgcat acctctgcgt tgagaagagt tgcacgagtt
    18061 gcggaatctg tgcaaaagta tgccctctgg gtgccctgga ggttatcgat gaaaaataag
    18121 tatgatgtcc tggtcatcgg gggaggtccg gcaggggcga tagctgcaaa aacagcagtg
    18181 gaaaagggac tctccgcgtg tattgtcgaa aagcggcctg ctatcggggc tcccgtacgc
    18241 tgtgccgaag gtatcgggaa agaggccctc cacgagttca tcgatccgga tccctgctgg
    18301 atctcggccg aaatgaccgg tgcgagtatt gtcgcccccg atggattttt aatgaaactg
    18361 gaatcccagt tagcgggcaa caaggtcggg tacatcctcg acagaaaaat tttcgaccgc
    18421 gagcttgtct ggaaagcagc ggaggccggg gcggatattg ccgtcaagac ccgggcatca
    18481 gcaccgataa tggaaaacgg gtttgtcaaa ggggcgaaac tggagagctg cgggcaggtc
    18541 atgagcgtta ctgccgaggt ggtcatcgct gctgatggtg tcgaatcaaa attttcccgg
    18601 tggtgtggta tcgacactac ggtccctgtc cgggagatta tgagttctgt ccagtatgtt
    18661 atggctgata tcgacattga tgctcactcg actgtttttt atcttggcaa tgaagtggca
    18721 cctgaaggct atctctgggt ctttccgaaa ggtgaacgtt ctgcgaatgt cgggataggc
    18781 atatccggga aaaaaagcgg tcagggccac cgggcacggg attaccttga caggttcgta
    18841 aaaaagacat tccccaaagg aaaaccgatt gaatatattg cggggggtgt atcagtctgc
    18901 cgccctcttg cctgcacggt agctgatggc ctgataattg ccggcgatgc agcccgggta
    18961 gtcgatcctc tcaccggagg tgggatctat aacggtatgt ataccggaag actcgcagcc
    19021 gaagtggcag ccgaatgcat tgcaaaagga aatgtctcga aaaaagccct catggtctat
    19081 gacaggaaat ggcgggaagc aaaaatgggc aaatccatcg aacgtaatta tcacataaaa
    19141 gaatacctga taaaactgcc cgatacgaaa ttaaacgaga ttattcattc tgtttcaaag
    19201 atcaatctta cggagttcag cacgctctct ttaataaaag aaataatccg ggtaaacccg
    19261 aaactcatgc tggagctggg tgtgctcgca gcatcaatcc gctgattttt taagttgttt
    19321 attgaggagc cgcaggcagt cactttcagc ttcttttttc gtaaagaccg tgaaaatatc
    19381 atttggctgg atttttacat tcccgcttgg tatgatcagt tcgccgccgg acctgcggat
    19441 ggcgatgaac acgaaatctt ttaccttgag ttcccgtatt tcatgtccca caataggagc
    19501 accctgttca gcaacaatct caaagatagt cccgcccgga atagatgcga gctgctggag
    19561 ctgggggttc tgggtccagt aatagagacg ggatgccacg agttcatcag gattctcgct
    19621 aatccttact cccacttctt tgaaaaagtc tgagtgagag gactggttga cgatcgaaac
    19681 aacattcggg acatggaatt tttttgcaag ccagcaggtc ataaggttga ccgaatcatc
    19741 gctggtcgtt gcaataagag catcagcccg gtcaataccg gcctcttcca gggtcgtttt
    19801 atccgtcgca tttcccgtta ctgccagaac atcatagtgc tcaaggacat cactgcaccg
    19861 ggcctcatcc tggtcgatta cgacaacatt gttggcatgt tcaaccgcct gttttgccag
    19921 gtttttcccg atacccccaa gccccacgat gataatgtac atgaataccc gttaccatca
    19981 tcggcattga aataatttgt gcattaacca ttccatgtga tctgcggtgt tgatgaggca
    20041 ggcaaaggat ccgtgctcgg acctatggtg gttgcggctg tcggaatacc ttcagaagac
    20101 gttttttctg atttggaagt tcaggattca aaattgctct ctcccaaaat gcgggaacgt
    20161 ctctatacca tcatcaggaa acggttcagg gtggcaacgg ttcgcattga cgcgcatgaa
    20221 atcgatgaga tccgcacggg tataaccatg aatgtctgtg ttgcccgtgc acatgcccag
    20281 gttatcgaca ggctttcacc atctgtggca tatgtcgatg cctgtgatgt aaacgcgttc
    20341 cgttatgcag acatggtaaa aagtttcctt gaccagagct gcgagattgt atctgagcac
    20401 cacgctgacc agaccttcag ggtggtctct gcagcaagta tcgtggcaaa ggttacccgc
    20461 gaccgggcga taagtacgct tgcaaaaaaa tacggaaata tcgggagcgg ttatccttcg
    20521 gacccggtca caattgcctt tctttctgaa tatttcgatg aacacaaatg ccccccaccc
    20581 attgcccgga agagctggaa gaccgtaacg accatgattg cccagaagca gcaaagccgt
    20641 ttatcagagt ttttctgatc cctcttctgc gttgtttctt cgtctgattg catcaagcca
    20701 ttgttttatg tgacctgcaa tacaatgaat aacgaatgaa ctggatcctc cctgttatcc
    20761 ttcttattgc tgtatatgca gcaatcgcat actatattca ctcgcgcaaa ctctgggagg
    20821 atcgcatcac cttctatggt cctattatgg ccatcaaaac gaacaggatc gggtttttcg
    20881 acaaattcat cgcattccgc acgtttttcc ggatctatgg gactgccggg gtagcagcgg
    20941 ttatcatcgt ttcggttttt atcaccctga tgttgtttat ctctgtccgt tacaccctgc
    21001 ttatccagcc ggagcccacc gggatctaca aaccgcaaaa catactgctc cttcccggta
    21061 tcaatgaata tgtcccctcc acgcttgctg tctggctggc cttcattatc accatcgccg
    21121 tgcatgagtt cggccatgca attctctgcc gggttgagaa tatcaaagtg aagaccatgg
    21181 gggtgctggt tgcggtcatc cccatcgggt ttttcgtcga gcccgatgaa gaggaacttg
    21241 acaggacccg cggaatggcc aagataagga tgtttggagc aggaatcacg aacaaccttg
    21301 tggtaggatt ttcgtgtttt atcctcctca tcctctgtat ggggctcgtt gttcctgtca
    21361 cccagccgat cattcacggg gtatacaaag attatcctgc cgctattgca ggaatttctc
    21421 caggatcgat agttactgcg gtgaacgggg tatccgtcac gaaccgtgcg gatgtttcaa
    21481 ctatcctgaa taccaccaag cctggcacta ttctcaccct taccgtggaa aaagattctg
    21541 tcagccggga ttacccgctt acgttatcgg cctggccggg ggatattccg gacaggacca
    21601 gtggatttat gggagtcgag tattatgacg gttctgcggt catgggagtc attcagggaa
    21661 tgttgtcacc aattggtttt ttccagttcc tgatcgtccc ctttgcatct gattccggtg
    21721 tccagttcct tcgcatactc gcctttgaga ctccggatat gacgtattac caggtcccct
    21781 ttgagggttt ctggggggtt atccacctgc tcttctggtg tgcatggata aatcttaatg
    21841 tcgggatatt caatgccatc cccatgatac cacttgatgg cggttatatc ttcaaagagg
    21901 gtgtagaccg gttacttgac cgccggggtc ttatgaaata ctcaggatat gtaacaggcg
    21961 ccgtcagcta cctgatgctg gttgtcctga tatcactgat tgcattaccc tacctgttgc
    22021 atatctgatt tttcagggat tgtttgatag tgggacacct gtcacacaaa tatatccctg
    22081 ctatatccct gcttattttt gtgtttttac aagatggcgg aaaaccggac ttaccggttt
    22141 tatccgtgat gcaacctctg ttaagggtat tgtaccgatc cgggaaccct gcagggcaac
    22201 catgaagccg gtatttccgg ctgccagctg ttcaactgct tccgcaccga aacgggtgcc
    22261 aaggatccgg tcgaatgcag taggagttcc tccacgctgc acatggccaa ggaccgttac
    22321 ccgggtctcg ataccgagcc ggttttcgat ctcttttcct aacaggttcc ctgctccaac
    22381 gaatttctcg tgtccgcact catcccggtc gcattcaggg actttggcag cggaaatgtc
    22441 ttcatggtga attccctctg caacaacgac tatggaatat ttttttcctg ccatatagcg
    22501 ggctttgagc tcacgacaga cctcgtccat cgtaaaacgt tcctcaggaa taaggaccat
    22561 gtcggctcct ccggctatac ctgccataac ggcaatccag ccggcattac gtcccataac
    22621 ctcaacaacc atgatcctgt ggtgggattc tgcggtagtg tggagccggt cgatcatttc
    22681 ggttactatg gaaaccgcgg tatcaaatcc gactgaactg tccgtcccgg aaatatcatt
    22741 gtcgattgtt tttggaatac cggccaggtg gataccggca ccggcaatat cagacgccgc
    22801 ggaaagtgtc ccatctcccc cgatcacgac aagggcatcg atacccgaag ttttcatggt
    22861 tttttgtatc ttcccgatgt catcaggatt tttgagcgga tccgttcgtg acgttcccag
    22921 gattgttccg ccatgggaga gtataccgga aacagaagca gtggtgagca cttccaggtc
    22981 cccctcaata agcccctgcc acccgttatg tattcccagt gtttttatac catgtccggc
    23041 ggccgccagt acaactgaac gaattactgc attgagcccg gggcagtccc cccctccggt
    23101 cataacggca atttttttta atggaatggt caatcctccc gagtcattgt ggatttttct
    23161 ctatgaatat ccgtcggtgt atgatacgcc agcagagaat aagttgtgcg agttctccgg
    23221 ccgtgatata ggtccggttt ttgaaaattg agatcggatg cacccgagtc tgctgttcga
    23281 tgctcagctc aagttttttg actacatgtt cgcttagttc accatcaata atcatctctc
    23341 cgatcttttg cccgttttgt tcgtttgtca gaaatttcaa cgagaaattg cgttctgaaa
    23401 gagacaggat cgaatagagg tccaacgaga gatcgatatt ctcaatactc tgtatcatcc
    23461 tgttctgtga gagcgtgacg cggtagacat tacgctcctt gccaagatcc ttcccgtatt
    23521 cagaataatc aatgccgatt accgcatcgg caaacatttt ctggggagca ataaagcgct
    23581 cgtattccgg ttcccgttcc gccatctcca gcagaacagc atcacgggaa tacccccgct
    23641 tttccatatc acggcggatc ttccagtcag attttaccgc ttttgacgga tccacaaaga
    23701 gagtaaaatc caggtacttg cgcagcgtgg gggtaaacag tgtgtgaagc ccttcaagaa
    23761 tgaggatttt ttgaggacag aagatgaccg ggggatcaaa aaccccggtc gaatgattat
    23821 ataccggctt ctctatggga actccacgtt tgagcaggat aagatcgtgt tccaactggt
    23881 caatccggtt tgcttccgga ttcagtgcag tcagaccctg ttctttgcgc ccctccctgt
    23941 cgagacgatg ataatcatcg agagtaatgg tggacacaag gtcagtacca aagacgctcc
    24001 ttatcccttc cgtgaacgta gttttccccg ttccgctgtc ccctgctaca cccagggtaa
    24061 aaatataagg agatgctgcg attgtctctt taaaattcag atagttcatt ccttaccacg
    24121 gtataggtgc atatctggta aaaacactat ggtactttta tcgcgtgata gtaaaaaggg
    24181 agcgaaggtg aaaaaaccgg ggttattatt ccgcgggttt taatgtggac tcccattttt
    24241 ccttttcgat cgggagtctg cgcttgctgg tatccttgag ttcatcctca gaaacatgag
    24301 gtacagcatc ctgaatatcc tttaatattg catctaccgc ctcgcgcgtc gggatatcgc
    24361 cgaggatgct ctcgtccata accggtcgtt ctgctgcttc ttcgggggtc tcatcagcag
    24421 ctccaaggaa tttagcaccc tggcggatca gctgtgaaat ctcaaacggg aaaataatct
    24481 tggttgcctg accattggcc attatcttta atgcgtcaag ggaaagtaca gtgattgctt
    24541 ttttatcgag tggtgctgcc ccgacagaga ggatgcggag accctgtgct tcaccctgtg
    24601 cctgcaggat tctggagagc cgttcgcctt ctgcgcgaag caccttgctc tgccgctcgc
    24661 cttccgcttc aaggatcatg ctctgccgga gaccttcagc cttgaggatg gccgagcgct
    24721 tctcaccatc agcgcgaagg atggcagcac gccgtgcccg ttcggcagcc gtctgctcgg
    24781 tcattgcatt cttgacggcc ccgatggggt ccacttcttt tatttcaacg cgttctactt
    24841 tgactcccca ctggtcagtt tcgcggtcaa gaatatcccg gagttttgtg ttgatcacat
    24901 cccggttata gaggacttca tccagctcca tgtccccgat aatcccccgc aggctcgtct
    24961 gggcgagagc caccgtggca agacggtaat ttccaacctc aaagaaggcc ttctcgggat
    25021 caattacccg tatatagatg attgcatcaa catttgtggg cgagttatcc ttcgtgatca
    25081 cttcctggct gggaatctct atcacggttg tccgcaggtc aagtttaatc gcttcgctta
    25141 cgaatggcac gatccagcga aaaccgggat tcatccgccc cacatattgc ccaagacgta
    25201 tctgaagccc ctgttcatac ggctggacaa tcacgacgcc ttttgcaaaa atggcgacga
    25261 tgacaaggat caaaaatata acgataagtg tctcgaaaac tgccatgtta ttccacctct
    25321 tctacaacta catgtacacc ctgtgaatca acaactttta ccttttttcc cggggggatt
    25381 tcacatccgg ttgagcgggc actccattca gtgcttgcaa taagaacctt accggaaatt
    25441 gttgttgcat caacacggat tttcacctga ccttccatac caacaaggga gtcgcggctg
    25501 atagttgtcg gactttcatt gggtgtgatc ttcccataca tccataccgt gaaccctgct
    25561 gcaccgatcg cgattatcac tcctgctaca acgccccagc cggaattgaa gacattcact
    25621 cccagcagaa ataaaatacc taaaaagatc atcacggtgg caggtaccgt agcaaaaaaa
    25681 cccgggctat atacttcaac caggagaaga agagccccgc tcatgataag aagccatcca
    25741 aggtcaattc caagcaggag taccatgatc aatgatcaga ataaacggat atatccttat
    25801 gggaagcaaa gtcagacaga gaagttggct gttatccggt acgcagcatg gcgatcttcg
    25861 atcctacggg aacacccact tcatcagcaa ctggtttgca tttcgctgcc ataaggtatg
    25921 cgataggggg aagattgctc atttcggaca gcgactcgaa gtttgccatc ccttttgcaa
    25981 taatgatggt gcaccgggaa atggccttgc gaagattatc cggaatattt tccatgcata
    26041 taccgatctc agcacctccc cccgttgtcg tgagaatatc gacgcaggtg tccagaccca
    26101 gctcctttgc atcagccagt gttgcatcat tgagaatcgg ggcctctttt accgcgagtg
    26161 tgatatggga attttgtgac ctgaggtatt ctataaagag gcgatcgaga acaatctcac
    26221 cacaattatc gcttaagtat acaacacggt cacagagcgg ggcgatttta tccgtatcgt
    26281 caataaaaaa cccccggtcg aattcctttt tgaaaaaatc ataaaaattt tcttccacaa
    26341 tgtgcccttt tacgccgtag tcaaaggtat tgccgatgac tgaagcaagg gcaaaatcgc
    26401 gaaatgttga gagttcacta cagatctttt tgcagacttc ttgtgcttgt tggttacccg
    26461 aagcttttag ttctgcaaac ggatcggaat tccctaaaac ttcatacgcc ctgcggtgta
    26521 tccggcttgc tatctgtgga tgactcagtg gcgctgaacg gatctcgttc atgatcctgc
    26581 tgcactctgc cattgttgtg tccgcaagag agggactggc acggcataat gaacattcga
    26641 ggttaacacg ggagagtaaa cagtcaatgc agtgttcagt aattttcata ccatcgacca
    26701 aaaaaacaat gtttagaaat ggggccactg agatttgaac tcaggtcaga agacccccag
    26761 tctcctagga tggccaggct accctatggc cccgctctct tactattggc tcatttcatt
    26821 tatttatctt gttatgaaaa tgccatttat cccaatcggg gaattttacg gacttgccgg
    26881 gacaacacgc cataaccctg catttcgaag actttttttt atttcaggat ttttttcttt
    26941 tagcgaaata ccctccaccg gcgcaaaaaa aaagatatgt atatatgttt caatcaggta
    27001 ttatgaatta caaagggtag tacagcaggt gccggactat ggttaaagag catattttta
    27061 cattcattga ttcaattcga tcgggagtag gggacgcaca gaagatcggg ggggcgatgt
    27121 gatgtatact atccttgttg tcgatgacag tccattcatt gttgacgtgt ttgtcactat
    27181 gctggaacgg ggggggtacc gcaccgttgc tgcatacgga ggagaagaat gtcttgagat
    27241 tttaaagacg gtaacccccg atctgatcct gctggacata atgatggaac ctgttgacgg
    27301 ctgggaaacc ctggaaaaga tcaaggaaaa cccccagacc aaggagatcc cggtgctcat
    27361 gctgactgca aaacagcttt caccggctga ggcacaggaa tacgggattt atatcgaaga
    27421 ttatgtgtta aagcccataa ctcaccgcga gctgtacgat gcgatagagc atgttctcca
    27481 ccgcagacag gcaatcaagt ctgatatgga tatagcccgt caatccggat ttgatcagga
    27541 gatcatctct gaatatgccc ggttaagtaa aaccatcgat gtcaacaaac gccttatgaa
    27601 gatactcgag actacctata atttcgatga ttcaaaagtc agggtcagtg aagagatatc
    27661 aaacgccatc aggaatatgg aaatcaatat caagttccag gaaagccggc tccagcagat
    27721 aaaaggggaa ttaaccggaa cacctgaaac caaataatct aaccctccgg atccattttt
    27781 ttatcaagta ccggatcaca ctggtactcc tgaattccct cactcccata gtgttgcggt
    27841 ttattgcgcc attcctgcag aaccattgcg agggagaaca atactctcca aacaaagtgg
    27901 taaagtgaat agtatatggc gaatctttat atagtttttg ggcattgtta taatgctgta
    27961 catcttagct tgtacggggt tcgtagctca gttcggtaga gcgcctggct tttaaccagg
    28021 tggtcggggg ttcaaatccc tccgggcccg ttgccacgac aaatcgtggg ttttttgcgg
    28081 tgatgcatct gagaggaaat ctattcatct gtgagggggt ttttcattga gcaccaagtt
    28141 gaatgtgttg aagcacgtga tggtgccaga ccataaaatc atgagtgagg aggaggtatc
    28201 tgcgctccta tcccactaca acataacttc ggaacaatta cctaaaattt tccatgacga
    28261 tccggcggtc aaaacaattg gcgcaaatgt tgacgatgtt atacgaatta tcaggatgag
    28321 tcatactgcc ggcagggcag aggcatatcg tctcgtagtc aagagaccga aaaaataatt
    28381 tttggggtgg tcagtctgat cgacagaagc attttatcga aggcgtattt ttcacgggag
    28441 catgttgcgc gccaccaact agactctttc aattatttcc taaaacacaa tcttcagaaa
    28501 gtggttaacg agcagaaggt tatcgagact gatatcgaga accgggggaa aaacaacgaa
    28561 cctgtatggg ttgaactggg cacgatcacg gttaaaaagc cgcttgtgag agaggcagac
    28621 gggtctcagg gagaattgta tccctgcgag gcgcggctgc gcaatcttac gtacgccgcg
    28681 ccgatccagc tcgggcttac ccttgttcat ggtaacgaac gccaggaacc cataattacc
    28741 acaatcgggc agctgcctat tatggttgga tcccaatcct gcaacctgtt tggcatggat
    28801 gatgcacaac gggtttcgca tggggaggat gcctttgatc ctggcggata ttttatcgta
    28861 aacggtactg agcgcgtgct catgaccctt gaggaccttg cgtccaataa gatcatgacc
    28921 gagttcaccg agcgttataa cgagcgcatt tacgttgcaa aagttttttc gcagttccgc
    28981 gggtaccgtg cactggttgt tgtcgaacgg aataagaaga acctgctgga ggtatcgttc
    29041 ccctctgtgg ccggtcacct taaattcgtc gacctgatgc gtgctcttgg catgaccaat
    29101 gaccaggaag tcgtgaacgc agtatctccc gatgaagata ttctcacgtt catgatgcag
    29161 aatttagaag agagcgaatg cgattctgtt gagggcggga ttatgtatgt cgggaagaaa
    29221 ctcgccccga accagacgaa agattaccag cggaaacggg ctgaattcgt actggacaat
    29281 tatctcttac cccatctcaa ctactcgatg ccggcaaact taaaggaagg cgacgagggt
    29341 taccaggaag ctgttctgtc agtgcggctg gctaagggtc acttcctggg acgtatggca
    29401 gaggcatgtt tcgatctggt gctggacaaa cgcagggtag atgacaagga tcactactcc
    29461 aacaaacgac tcaaacttgc cggtgacctg atggaggatc tcttccgtat ttccttgaac
    29521 cggcttacgc gggatatcaa ataccagctt gaacgtgcca gtatgcgcca ccgtgacctg
    29581 tcgattgcga ccgcggtacg ggcagatgtc cttacagaac ggctcttaca cccgcttgca
    29641 accggtaact gggtaggcgg cagaacaggc gtctcccagc tgctcgaccg cgtcgaccat
    29701 atggctgtac tctctcacct tcgtcgtgtc atctcgccgc tttcgcgttc tcagccacac
    29761 ttcgaggccc gtgatcttca ccctacccag tggggacgga tctgtcccag tgagacacct
    29821 gaaggcccga attgtggtct tgtcaagaac tttgcccaga tggtcgagat atccaaaggt
    29881 gttgatgatg aggcggaagt tatgcgactc ctgcatagcc ttggtgtaga acatattcgg
    29941 ggtgaaactc gatgaagaaa tcacgcgtgt tcgttgacgg agcacttata ggacttgagg
    30001 atgatgcaaa agcgctggtg gcaagcatac ggtccatgcg ccgacaggga gccatttcat
    30061 ccgagctgaa cgtctcacac aaggaataca atggggatgt cgtgattctt accgaccgcg
    30121 gccgtgcccg gcgccctctt attgtcctga agaacggcga acccctcatc accagtgaag
    30181 accttgcaaa actggcaaga aaagaaattg atgtcagttc atttatccag cgtggcctga
    30241 ttgaatttat cgatgcggaa gaggaagagg atcttctcat cgccatgaac ccggagaatg
    30301 tgaccccgga acacacgcac atggagatcg acccttccct gatactcggt atcggtgctg
    30361 cacatgtacc gttccccgag cacaatgcaa gtccgcgtgt aaccatgggt gcaggaatgg
    30421 tcaagcaggc tctcggtttt ggatgcgcca atatgaagct ccggcccgac acccgggggc
    30481 acctgctcca ttacgtgcaa aaacccttaa cccataccca gacttccgaa ttaatcgggt
    30541 ccgatgaccg tcccgcaggc cagaactttg ttgtcgcaat cctgtcctat gaagggtaca
    30601 atattgaaga tgcacttatc tttaacaagg catccataga gcggggactc gggcgttccc
    30661 atttcttcag gacctatgaa ggggaagaac gccgctatcc cggtggccag atagaccgga
    30721 tagaagtgcc tgaagaagat gttgccggtg ctcatggtgc cgattcgtac aagaaccttg
    30781 acgaggacgg cgtgatcaat cctgagaccg ttgttgcgga aaaagatgtg ctgataggca
    30841 agacttcccc tccacgtttc cttgaagaac cttcaggtga gcttatcgct gtcgagaaac
    30901 gtcgcgatac ctcggtaacg atgcgcagca atgagcatgg tattgtcgat accgtcatca
    30961 tcaccgaagg agagaacagt tcccggctcg tcaaggtgcg gacccgcgac cttcgtatcc
    31021 ctgaagtggg agacaagttc gcatcccggc atggtcagaa aggtgtcgtc gggctcatca
    31081 cccaccagga agacatgcca ttcaccgaga gcgggctctg tcctgatctc gttatcaacc
    31141 cccatgccat tcccagccgt atgactgttg gtcacatgct tgaaatgatc ggcggcaagg
    31201 tcggagctct tgaaggacgg cggattaatg gtacagcatt ccgtggagag agtgaagcga
    31261 atatacgtgc ctccctcaag aaccttggat tttcgcatac cggacgcgag ataatgtatg
    31321 acggcattac cggcaaacaa ttcaaagccg acgtgtacgt gggtgtcatt tactaccaga
    31381 aactctacca catggtgtct tccaagatgc atgcacgctc ccgcggtcct gtgcaggtcc
    31441 ttacccgcca gccgacagaa ggcagggcac gggaaggcgg tctccgtttc ggggaaatgg
    31501 aacgcgatgt tatgatcggg cacggtgctg cgatggcact caaggaacgt ttgctggatg
    31561 aatctgataa ggtccaggaa tacgtctgtg cccactgcgg aatggtcgca atgctggacc
    31621 gcaaacgcaa catgacacgg tgcctcgcat gtggcaacga gacagatatc tattctgtcg
    31681 aaatgagcta cgcctttaag ctcctgctgg atgagatgaa gagtatgggg attgccccaa
    31741 gattaagact gcaagacgtg gtctgaacaa aaaggtgtgc catgccaagc ccaaaacgaa
    31801 ttggtcaaat tgaatttgga ttattatctc ctaaagaaat ccgcgagatg agtgtaagga
    31861 agatcatctg ggcggatacc tatgatgatg acgggttccc gtatccccag ggtctgatgg
    31921 acctcaacct cggtgtcatc gatccgggtc tcaggtgcaa gacgtgcgac cagaaagcaa
    31981 gcgagtgtcc cgggcatttc gggcatattg agcttgcaaa acccgtaatc catgtcggtt
    32041 acactcgcct gatcagaaaa ctgctgagag tcacctaccg ttcctgcggg cgtgtcctcc
    32101 tgagtcctga tgaaatagaa aaagtggttg ggactgaaga tgatcagact ggcgacctgc
    32161 tttcggaaaa ggacgtgaaa aaggaacgga tctgcccgca ctgcggagat cagcagctca
    32221 agattaactt cgagaaaccg accacatttt cagaagtgat ggtagaggat ggaaagaaag
    32281 tcgagcacaa gctcacgcct gctgacatca gggcccggat ggaaaagatc cccaatgagg
    32341 acttaacccc cctgggtatc aacccggatg tcgctcgccc ggagtggact atcctcacgg
    32401 tccttcccgt cccgccggtt acaatgcgcc cgtctattat tcttgaaaac ggacagcgtt
    32461 ctgaagatga ccttacccat aagctcgtgg atatcatccg tattaaccag cgtttcaagg
    32521 aaaaccagga tgcaggtgct ccccagctga ttatcgaaga cctctgggaa ctgctgcaat
    32581 accacgtcac cacatacctt gataatgaag tggcgggctg cccacctgct cggcaccgca
    32641 gtggcagacc cttaaagaca ctttcccagc gcctgaaagg aaaggacggc cggttccgtg
    32701 gctcgctctc cggcaagcgc gtgaatttct catcccgtac ggtcatttcc ccggacccga
    32761 acctttcggt ttcccaggta ggtgtcccgc gtgccgtagc aaacgagatg actattccgg
    32821 taagagtgac aacccacaac atcgaggaac tcaagcagat cgtgagaaac ggccctctcc
    32881 gccccgatgt caatgcaccc tgcggggcaa attatgtaat ccggcctgac aaccgaaggc
    32941 tcaggcttgc cgacggcaat cttgataccg ttgctgacct gatcgaaccc ggctacacgg
    33001 tagaacgaca gctccgtgac ggggatgtgg tccttttcaa ccggcagcca tccctgcacc
    33061 ggatgagcat catggctcac cgcgtcaaag ttatggaagg caggacattc cggctgaatc
    33121 cggcagtttg tccgccatac aacgcagact ttgatggtga tgaaatgaac atgcacgtac
    33181 cgcagacgga agaagcccgt gctgaggcga ctatcctcct gtcagtgcat gaaaacattc
    33241 tctcaccccg tttcggtggt ccgatcatcg gcggtatcca cgaccacgta tccggtatct
    33301 tcatgctcac gcacggtatg cgctggttca ccaaggaaga agcactctac ctgctccggt
    33361 atgcacgtgt tgaacacatg cccaagcccg gcaaggaaga aaatagtgtc ccgtactgga
    33421 gcaacaagca gatcttctcc atgatcctgc ccgaccagct gaacatggtt ttccatgcaa
    33481 gttcgtgcca gaactgcgat acctgtaaaa aagagatgtg tgagcgggac gcgtatgtaa
    33541 ggattattga tggtatttta gaagccggca cgatcgacaa gaaggcaatc ggcgcattcg
    33601 atggccagat cgtcaaccgc atcatccgtc actacagcat gcgtcgtgca gcagatttca
    33661 tagatgacct gaccaagcta tcgatccgtg caatcatgtt cgacggtttc tcgttcggta
    33721 ttgatgatga agacttatca aagaccgaat acgcccagat cgacgaagtg cttaccgctg
    33781 cggcgtctga cgtcacgcga cgcatcaaga tttatgaaga cgggcagctt gaacccatgc
    33841 ccgggcgtac ggttgaagag accctcgaga tgcagatcat gcaggtactg ggaaaagccc
    33901 gtgaccagac cggtcagatc gccggccgtc accttgggct tggtaacagt gctgtcgtca
    33961 tggctgtatc cggtgcgaga ggttcgatgc tgaacctgac ccagatggca ggatgtatcg
    34021 ggcagcagtc tgtccgtggt gagcgtatcg tacgtggtta tgatgaccgg acgctcccgc
    34081 acttcaagaa aggtgaccgc ggctccgatg cccacggatt catcgcccac agttataagg
    34141 gtggtcttaa ccctacagaa ttcttcttcc acgcgattgg tggcagggaa gggcttgtcg
    34201 atactgcagt ccgtacttca cagagcgggt acctgcagcg taggatgatt aatgccctgc
    34261 aggacctcaa ggttgcctat gacggaaccg taaggtcaac cggtggaaga atcatccagt
    34321 tccagtatgg tgaagacagc acagatccca caaagagcgc ttttggcgac cctgtcgatg
    34381 tcaaaggtat tgtagagagt atcttaaaag aggaagtctg atgccgc
//
LOCUS       DASRLV010000049        12766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_502150,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000049 DASRLV010000000
VERSION     DASRLV010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 12766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12766
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502150"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VFC43_02060"
     CDS             <1..513
                     /locus_tag="VFC43_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HZK30006.1"
                     /translation="FARELLADPKERAEHTMLVDLARNDLGRVCRFGTVEVEEFMGIE
                     KFSHVQHIVSTVRGTLQDHLDCYDAFKSCFPAGTVSGAPKIRAMQIIDEEESQNRGIY
                     AGAVGYIGFDRNLEFAIAIRTVIVRNGLAHIQVGAGIVADSVPENEWRETESKASAML
                     RAIERAGVNS"
     gene            510..1091
                     /locus_tag="VFC43_02065"
     CDS             510..1091
                     /locus_tag="VFC43_02065"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HZK30007.1"
                     /translation="MKVLIVDCYDNFTFNLFQQVGKLGGNPVVMTCDTPLEQLKKTPC
                     SRIILSPGPGTPADSGVCPEVLETMSLTIPTLGVCLGHQAICTTFGGKVTRAEHLMHG
                     KTSEIQHDGLGIFSGITSPFTATRYHSLVAKNDSLPPDLQVSATSLDDHYVMGVRHTR
                     YPIEGVQFHPESILSPDGDRIIRNFLYGKPVAQ"
     gene            1088..2107
                     /gene="trpD"
                     /locus_tag="VFC43_02070"
     CDS             1088..2107
                     /gene="trpD"
                     /locus_tag="VFC43_02070"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HZK30008.1"
                     /translation="MTFREALIKLSAREDLKAAEAAGVMRIILEGKASQAQIGAFLLA
                     LHMKGETAEEISAFARVMRDTAVTIKPVTGRMLVDTCGTGGDGARTFNISTASAIVAA
                     GAGCLVVKHGNRSVSSRCGSADVLMALGVKVGINPEAQADIIQRAGIAFFFAPTHHPA
                     VKYVMTARQEIGCRTVFNILGPLVNPAGAQAQVLGVYCPDLTGTLAKVLQILGLSRAM
                     VVHGSGLDEITTTGETVVAELRNGLVRRYTIHHDTFGIAPAQLQDLAGGTPEQNARII
                     RDILEGEHGPPRDIVLMNAGAALYVGGLSRDLHEGIRLSAASIDSGNASARLDALIEA
                     TQGVA"
     gene            2104..2859
                     /locus_tag="VFC43_02075"
     CDS             2104..2859
                     /locus_tag="VFC43_02075"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HZK30009.1"
                     /translation="MILDEIIHRTRKRVDLLPQVFFENSTRNHVSLVKAIRNRNTRNA
                     IIAEIKCASPSRGIIRRNVDMAVMAGALATRGCVALSVLTEPYYFGGSGQDIAKVKSA
                     VSIPVLRKDFIIDERQVAESLALGADAVLLIAEVLKERLPVFVDLALTYGLEPLVEVH
                     NRTQVETALSTNAELIGINNRNLSTLMIDRSATRLLSDSIRSAGRLIVSESGMRTGDD
                     IREMKPYCDAFLIGSSIMAHERPENKLEEFVCA"
     gene            3063..3398
                     /locus_tag="VFC43_02080"
                     /pseudo
     CDS             3063..3398
                     /locus_tag="VFC43_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
     gene            3429..4614
                     /gene="trpB"
                     /locus_tag="VFC43_02085"
                     /pseudo
     CDS             3429..4614
                     /gene="trpB"
                     /locus_tag="VFC43_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
     gene            4598..5389
                     /gene="trpA"
                     /locus_tag="VFC43_02090"
     CDS             4598..5389
                     /gene="trpA"
                     /locus_tag="VFC43_02090"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HZK30010.1"
                     /translation="MIGRIERAFTVKKHPLFIGFTVAGDPDKPTCIHIARALIAGGTD
                     ILELGIPFSDPVADGETIQKADERSLAAGTTPSTVFEIVREIRKESDIPIVFLTYYNI
                     VYRRGIEKFCQEAEESGVDGILIADMPVEESDEVCEASFRHNIDPIFLITRTTSDERI
                     KKIIKRARGYLYLVTVLGVTGVRENVSSGAIDLLERVRKQTDLPLALGFGISLPEHAK
                     TCSAAGADGIIVGSAIVDIVERNLNDPDVMERALKEYVMKMKNTP"
     gene            complement(5397..5906)
                     /locus_tag="VFC43_02095"
     CDS             complement(5397..5906)
                     /locus_tag="VFC43_02095"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HZK30011.1"
                     /translation="MAEFVCDLCGKCCVSLGPLITIERQLNEQDYYCHSKIDRSLFLA
                     HVDPEYSEEIADVYSGESETPSRAEKNPCLFLRKSRQGAGNHCAIYSTRPKVCRDFRC
                     YRMLIYNTEGKVSGRVMGKNTLRTEDTMLEDLWRKHISPVPHANPVLWKEKVSAILAE
                     HGYKADVVG"
     gene            6078..6368
                     /locus_tag="VFC43_02100"
     CDS             6078..6368
                     /locus_tag="VFC43_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HgcAB-associated protein"
                     /protein_id="HZK30012.1"
                     /translation="MAKKTSSCGCSPQESDFVCSPGSGCRVEAVLSVDERGQMVVPKD
                     IREKALIKTGDKLALISWEKNGSICCLALIKVENLNGMVKNVLSPLMHDIDS"
     gene            6387..7421
                     /gene="hgcA"
                     /locus_tag="VFC43_02105"
     CDS             6387..7421
                     /gene="hgcA"
                     /locus_tag="VFC43_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury methylation corrinoid protein HgcA"
                     /protein_id="HZK30013.1"
                     /translation="MTSPRPTGCSCTPGANGSTSSTYTTDSTLTFANRLDHFLARWGI
                     NRNGHRVDPGLYRLGNPTGESPVFASANYTLSFDALRSALAGHDAYILVLDTKGINVW
                     CAAGKGTFGTDELVGRIGTTGLGRTVTHRKIIVPQLGAPGISAHDVQRQSGFRVEYGP
                     VRAGDLPEYLKKGTTTPEMRRVKFPVNDRIILSPVELVHIALPAIVITAGLWFLAGPV
                     ASLSAIFAVVAGTVLFPVLLPFIPTKDFSTKGLLLGGIGSIPFAGAFYSDTSLPGWAR
                     LASAITPLLIIPAVTAYLSLNFTGSTTFTSRTGVRKEIFRYIPVMTLMAVSGVILGIA
                     LGLTRLMGVI"
     gene            7421..7759
                     /gene="hgcB"
                     /locus_tag="VFC43_02110"
     CDS             7421..7759
                     /gene="hgcB"
                     /locus_tag="VFC43_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042192 - methylmercury biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury methylation ferredoxin HgcB"
                     /protein_id="HZK30014.1"
                     /translation="MFDSYTETTLHYHQDLCINCKRCTQVCPHGVFSEGAEHAELVRP
                     SACMECGACALNCPVQAIEVKSGVGCAWAMISAALRGKDMDSGECSCGGAEGSCCGVQ
                     EEKSSCCQDK"
     gene            7756..8184
                     /locus_tag="VFC43_02115"
     CDS             7756..8184
                     /locus_tag="VFC43_02115"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HZK30015.1"
                     /translation="MTAKTRVLFVCTANAARSQIAEGLLREKYGDRFEVFSAGTHLAK
                     VSVRAIEVMREIGIDISHHRSKTLAEYSGVSFDLAVTLCDNAHAICPVIYGAKKTIHR
                     GFADPHLIPGTDEVILDGYRRVRNEIASWIDEEFGDFSQE"
     gene            complement(8400..9239)
                     /gene="truA"
                     /locus_tag="VFC43_02120"
     CDS             complement(8400..9239)
                     /gene="truA"
                     /locus_tag="VFC43_02120"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HZK30016.1"
                     /translation="MEKNQTVHTARPASMRLAFRLSYLGSRFYGSQMQASERTVEGEF
                     VAACQRLSLFSDWREAGFQFAGRTDRGVHACGQVAAFTTDEPERAQTVINTQLPPDLW
                     CTASAVVEPEFHPRYDAKSRTYRYYFARKLKSPEAMHRAARNFLGSHNFSNFARVKDK
                     NPYRNILAIRVGNEEGFVFLEVQAESFLWHQVRCMASALILIGEEKADEGSISGLLEA
                     EAKRPLQPAPAEGLILWDTDCGIAWTPVAAEGRSGAFIDHLIQHHTLMEKVCRVLRAP
                     HPD"
     gene            complement(9194..9871)
                     /locus_tag="VFC43_02125"
     CDS             complement(9194..9871)
                     /locus_tag="VFC43_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZK30017.1"
                     /translation="MLICDLHVHTNFSKDGESSVEDILKKAENVGLDVIAITDHDTID
                     GAKKALSCSTTVLVIPGIEVSTKQGHLIVLGITEIIPSGLDVVDTVEIARKLGGLLIL
                     PHPYHVWRHGVARRKKAGMTVVDAIETFNSRYIVGSANRKAARIARKLGKPCVAGSDA
                     HHAKFVGYGRTFIDAERNIPSILAAIRAGKVNCGGRQTPLRTYTHQSLNNTWRKIKRF
                     TRRVRLR"
     gene            9913..11325
                     /locus_tag="VFC43_02130"
     CDS             9913..11325
                     /locus_tag="VFC43_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase-like protein"
                     /protein_id="HZK30018.1"
                     /translation="MRILLTTGAATEEVVKSAAASVDAEVVVTGKIASFLTPYMLHGL
                     IQKGSYDLVIVPGMCTASFEQVEQETGVPIYRGPRHAADLAFILTIVSSVTLSRTIPA
                     DDFLASNKAHDARKVLDKREKSAECDFIIRGTKIGGTSRMKVLAEVMDAHLHEDIRSI
                     VERYFAAGADIVDLGFGFDAAPADVTRVFSRLEGIDRILAVDTQEPDLIRAALGRADL
                     VLSLQERNIPEVGEAIAKVGAAAVVVPGENSLKKNLTLAKRAGISCIIADPLLQPVGS
                     GLVASLKNFKKYGFPLFFGAGNVTELIDADSIGINALLAGMAMETGAAVIFTSEHSDK
                     TKGSVSEMRKATEMMALSANRPYPKDLGIDLLVLKEKRRRREPPLEYDTAIPAITMPD
                     RIEYDPKGNFRIGIEGDQIVAVINDRAIRGKHWQDVLHTILSHDNVSLLDHAGYLGRE
                     LYKAELAIRFGRSFEQDGEF"
     gene            11430..11711
                     /locus_tag="VFC43_02135"
     CDS             11430..11711
                     /locus_tag="VFC43_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZK30019.1"
                     /translation="MAVQARASHILVKTEDQANQVLKRLADGEEFDKVARRFSSCPSG
                     KNGGDLGWFGKGQMVPEFEKVAFENEAGKVVGPVKTQFGYHVIKITGRK"
     gene            11730..12548
                     /gene="budA"
                     /locus_tag="VFC43_02140"
     CDS             11730..12548
                     /gene="budA"
                     /locus_tag="VFC43_02140"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HZK30020.1"
                     /translation="MDTKFFLGIGLAIVLVFCSAAVYSSFTRFPTASAIPEGQEILYQ
                     VSTIDALMQGVYDGVQPVGEIKKHGDFGIGTFDALEGEMIVLDGVVYQAKSDGVIYSV
                     ADNTTTPFATVTYFAPDFNTTTGRLMNFSEFSTEMNTRLPSQNMIYAVRMKGTFPHMK
                     VRAIPAQQKPYPTLTDAAKNQSVYTYDDARGTVVGFYTPVFFKGINVVGYHLHFISDD
                     RHSGGHILDFEVPVNTRVEYDITPEFKMLLPTSGAFIEVDLSKDLSQGLARVES"
BASE COUNT         3132 a         3179 c         3396 g         3059 t
ORIGIN      
        1 tttgcccgtg aacttctggc agatcccaag gagagagctg agcatacgat gctcgttgat
       61 cttgcaagaa acgatctggg acgggtatgc cggttcggga cggttgaagt tgaagaattc
      121 atgggcatcg agaaattttc ccatgtgcag catattgtgt cgacagtcag gggcacactc
      181 caggatcacc ttgactgtta tgatgcattc aaatcctgct ttcctgcagg taccgtatcg
      241 ggtgcgccaa aaataagagc aatgcagatt atcgacgagg aggaatcaca gaaccggggg
      301 atctatgccg gagctgtcgg ttatatcggt tttgaccgga acctcgagtt cgctattgca
      361 atccggactg tgatcgtacg caatggcctg gcgcacatac aggtgggggc aggaattgtc
      421 gcagattctg tgccggaaaa tgaatggaga gaaactgaga gcaaggcatc ggcaatgctc
      481 cgggcgattg agcgggccgg ggtgaattca tgaaagtcct gatcgtggac tgttatgaca
      541 acttcacctt caacctgttc cagcaggtcg gaaaactcgg gggcaacccg gttgttatga
      601 cctgcgatac cccgctggaa cagctgaaaa agaccccatg cagccggatc atcctctcgc
      661 ctggaccagg aacacccgca gattccgggg tctgtcctga agtactggaa acgatgagcc
      721 tgacaattcc cacgctgggt gtctgtctcg gacaccaggc gatatgtact acatttggcg
      781 ggaaagtcac ccgggcagag cacctgatgc acggcaagac ctcagaaatc cagcacgacg
      841 gactgggaat tttttccggg ataacctcac cgttcactgc aacaagatat cattcccttg
      901 tagcaaaaaa cgactccctt cctcctgacc tgcaggtctc tgcaaccagt ctcgatgatc
      961 attacgtgat gggggtccgt catacccgct acccgattga aggtgtacag ttccacccgg
     1021 agagtatctt gtctcctgac ggcgatcgga ttatcagaaa cttcctgtat ggaaagccgg
     1081 tggcacaatg acgttcagag aggctctcat aaaactttca gcacgcgagg atctcaaagc
     1141 tgctgaagct gccggggtaa tgagaataat tctggagggt aaagcgtccc aggcacagat
     1201 cggtgctttt cttcttgcac tgcacatgaa gggtgaaact gcagaggaga tatctgcatt
     1261 tgcccgggtg atgcgggata ctgcagtaac tattaaaccg gtgaccgggc ggatgcttgt
     1321 cgatacctgt ggtaccgggg gggacggtgc ccggacattc aacatcagca cagcctcagc
     1381 cattgttgcg gcaggagctg gctgtctcgt ggtcaaacac ggcaacagga gtgtgagtag
     1441 caggtgcggc tcagcggatg ttctcatggc actgggtgta aaagttggta ttaatcctga
     1501 agcacaggct gatattatcc aacgggctgg catcgcattt ttctttgccc cgacacacca
     1561 cccggcggtg aaatatgtca tgactgcacg ccaggagatc gggtgcagga cggtttttaa
     1621 tatcctcggt ccgctcgtaa acccggcagg agctcaggcc caggttctcg gggtctattg
     1681 tcctgactta accggaactc tcgcaaaagt gctccaaata ctgggattat cccgggctat
     1741 ggtcgtgcac ggatcaggac tcgacgagat taccacaacc ggtgagaccg tagtcgccga
     1801 gctcaggaac ggtctggtcc gcaggtacac tatccaccat gacacattcg gtatcgcacc
     1861 cgcacaactc caggatctcg cggggggaac tccagaacaa aatgcccgga tcatccgtga
     1921 tatcctggag ggggaacatg gccccccccg ggatattgta ctgatgaacg ccggggctgc
     1981 actctatgtg ggggggcttt cacgggatct ccatgaaggg atccggcttt ctgccgcttc
     2041 catagattca ggaaatgcat ctgcccggct tgatgccctc attgaagcaa cgcagggtgt
     2101 ggcatgatcc tcgatgaaat aattcatcgt acccggaaga gggttgacct gctgccgcag
     2161 gtctttttcg agaacagcac ccggaatcac gtcagccttg tcaaggcgat acggaacagg
     2221 aataccagga atgcaatcat cgccgagatc aaatgcgctt ctccgagccg gggcattatc
     2281 cgacggaacg ttgatatggc tgtgatggcc ggagctctcg ccacaagggg atgtgttgca
     2341 ctttcggtcc tgacagaacc ttattacttc gggggatcag ggcaggatat tgctaaggta
     2401 aaatctgcag taagtattcc ggtcctgcga aaggacttca ttatagatga gcggcaggta
     2461 gcagaatcct tagcgcttgg cgctgatgcg gtactcctga tcgcagaagt gcttaaagag
     2521 cgactaccgg tatttgttga tctggccttg acatatggtc tcgaaccgct ggttgaagtg
     2581 cataacagga cccaggttga gacagcactg tccacaaatg cagaactgat cggtataaac
     2641 aatcgtaatc tttctacgct tatgatagac cgttccgcaa cccgccttct ctcggacagc
     2701 attcggtccg cagggaggtt gatcgtatct gaaagcggca tgcggacagg agatgatatc
     2761 cgggagatga aaccttactg cgatgcattc ctgatcggtt cgtcgatcat ggctcatgaa
     2821 cgtcctgaaa ataaacttga ggagttcgta tgcgcataaa gtgctgcggt atcacccgcc
     2881 cggaagatgc acgatatgca gagagttgcg gtgcacacgc tatcggagtg gttgtttttt
     2941 cggggggtgt ctcccgtcgc atggtttctc ccgaccgtgc acgggaaatt ttcgatgcgg
     3001 ttggtccctt cacggcgaca gtcgcagttt cgcacacaaa atctgaagaa gacctttggg
     3061 aaatgctcac actcaaacca tctgccatcc agatatcaca cccattcgtg ttcgatgaag
     3121 atccgggtgt tgtaataata cgggttatcg ggctgggtga cccactgccg gatgactgcg
     3181 atgctgttat cgtcgatgat agtcatgggc ggggtaaatt atttgatctt tcttacgcac
     3241 ggaatgtagt ccgacatgct aaaatcccgg tcatactggc aggcggactc acgccggaaa
     3301 atgtggcaga tgccatttcg caggttcacc catatgccgt ggatgtcgca agcggtatcg
     3361 agtcctcacc gggtatcaaa gaccataaga aaattgctgc attcattaca gcagcacgga
     3421 gtggtaatct gacagcaaaa ggaagatatg gaaaatacgg cgggcagtac gtctccgaga
     3481 cactgatgag tgcacttatc gaactggagg acgcctatgg cagggcctgc agggatcata
     3541 agttttccag caatcttaga gcattacaaa ctgaatatgc cggacgtgag acgcccctta
     3601 ccttctgtcc taatgcctca cgggaactcg ggtgtaagat atatctcaaa cgcgaggacc
     3661 ttgtgcatgg tggttcgcat aaactgaata atacccttgg acaggcactg ctcgccaaaa
     3721 agatgggcaa gaaacggctt atagccgaaa ccagtgccgg ccagcacgga gtagccaccg
     3781 cgatagtcgt gtgccgcgct cgggataccc gtcgaagtat atatgggcga gatcgacaca
     3841 cagcgacagg cgctcaacgt cttccgtatg gagctcatgg gggcaaaggt tatcccggtc
     3901 aaatccggga cacgaacact caaggacgct atcaatgaag ctctccgcga ctgggtggca
     3961 aacgtccgtg atacctatta cctgattggc tcggttgtcg gccctcaccc gtacccgctt
     4021 atggtccgga atttccaatc agttatcgga agggaagccc gggagcagat agtaaaaaaa
     4081 gaggggcgac ttcccgatgc ggttgttgcg tgcgtgggag gtggatcgaa tgcaatcggg
     4141 atcttttacc cgttcctgtc cgatgatgtg gaattgattg gtgttgaagc cgggggtgaa
     4201 gggattccca ccggcaaaca ttctgctact ctttgtgccg gggatactgg tgttttgcac
     4261 ggtacactct cgtacctcct gcaggacgaa aatggccagg tccttccgac ccgcagcatc
     4321 tcagctggtc ttgactatcc cggcgttggt cccgagcatg cgatgctcag ggacaacaac
     4381 cgtgtggttt acacagcggt aaaggatcct gaagtactgg acgcattcag gtttctttca
     4441 aaaaccgagg gtatcattcc tgcacttgag tctgctcatg cagtggcgta cgtcatgaag
     4501 aatgcagagc ggtttgataa ggacaacatt gtggttatca acctttccgg tcgtggggac
     4561 aaggatgtgg ctgaagtggc agcaaagagg agagtacatg ataggacgga ttgaacgggc
     4621 ttttacggta aaaaagcacc cgttatttat tggttttacc gtcgcgggag atcccgacaa
     4681 accgacatgt atccatattg cccgggctct gattgcagga ggaactgaca tccttgaact
     4741 gggtataccc ttttctgacc cggttgcaga tggagaaacg atccagaaag ccgacgagcg
     4801 atcgcttgca gccggaacaa cgccttcaac ggtgtttgag attgtcaggg agattcgcaa
     4861 agaatccgat atccctatcg ttttcctgac ctattacaac atcgtctacc gccgtggaat
     4921 tgagaagttc tgtcaggaag cagaggaatc gggtgtcgac ggcatcctga tcgccgatat
     4981 gccggtagag gagtcagatg aggtatgtga agcttcattc cgccacaata ttgatccgat
     5041 cttcctcatc acccggacta catctgacga gaggatcaaa aagatcatca aacgggcgag
     5101 aggttatctc taccttgtga cagttctggg ggtaaccggg gtgcgggaaa atgtctcatc
     5161 cggtgcaatc gatctgctgg aacgggtcag gaaacagaca gacctcccgc ttgcgctcgg
     5221 gtttgggatc tccttacctg aacatgcaaa aacctgttct gctgccggtg ccgatggtat
     5281 tattgtcggc agtgctattg tggatatcgt ggagagaaat ctaaatgacc cggatgtgat
     5341 ggaaagagct ctgaaggagt atgtcatgaa aatgaaaaat accccatgat acagcgttac
     5401 ccgactacat cggccttgta tccgtgttcc gcgagaattg cgctgacttt ttctttccat
     5461 aaaaccggat ttgcatgggg aaccggagat atatgtttcc tccagagatc ttcaagcatt
     5521 gtatcttcag tacgaagggt attttttccc atcacccggc cagagacttt gccttcggtg
     5581 ttatagatga gcatccggta acagcggaaa tcccggcaga ccttcggtcg ggtagaatat
     5641 attgcacaat gatttcctgc accctgccgg ctcttgcgca ggaacaagca ggggtttttt
     5701 tccgcacggg aaggcgtctc actctctcct gaatacacat cagcaatctc ttcactgtac
     5761 tccggatcga catgggcaag aaagagcgag cggtcaattt tactatgaca ataataatcc
     5821 tgttcattca gctggcgctc tatcgtgata agggggccaa gactcacgca gcatttcccg
     5881 cacaggtcgc atacgaattc agccatactt taatgagagt atagtttcgc ggggaaaaag
     5941 ggattgcgct ctgtcgttac ctgtccaggc agcggatgaa ccggaatgtt cagtcttaaa
     6001 gtgataaaaa atttgatttt tctgaatttt ctgaaaagta taaatatttg gaaacccata
     6061 cttgtatgag aacttgtatg gcaaagaaaa ccagcagctg cgggtgcagc ccgcaggaga
     6121 gcgacttcgt atgttcaccg ggttccgggt gcagggtaga ggctgtcctg agtgtggatg
     6181 aacggggaca gatggtagtt cccaaggata tccgtgagaa agccttgata aaaacaggcg
     6241 acaaactcgc gctgataagc tgggaaaaga acggtagcat atgctgtctt gcgctcataa
     6301 aagtcgaaaa tctgaacgga atggtaaaaa atgtgctaag tccgctcatg catgacatag
     6361 attcctaaag gaggcaggtt ttttttatga catctccccg gccaacgggc tgctcatgta
     6421 caccgggtgc gaatggctca acctccagca catacactac tgacagcacg ctcacgtttg
     6481 ccaaccggct cgaccatttc cttgcccgct ggggtataaa ccgcaacgga caccgcgttg
     6541 acccgggcct ctaccggttg ggcaacccga ccggagaatc cccggttttt gcctcggcga
     6601 attataccct gagcttcgat gccctgcgtt ccgcacttgc aggacacgat gcatacatcc
     6661 ttgtactgga taccaaaggg ataaacgtct ggtgtgccgc cggaaaaggt actttcggta
     6721 ccgatgaact ggtcggccga atcggaacaa ccggacttgg ccgaaccgtt acccaccgga
     6781 aaattattgt cccgcaactt ggagcgccgg gaatatccgc acatgatgta cagcgccaat
     6841 caggtttcag ggtcgaatat ggcccggtga gagcaggaga cctccctgaa tacctcaaaa
     6901 aaggaacaac gacaccagag atgcgcaggg tgaaattccc ggtcaacgac cggatcatcc
     6961 tgtcacctgt tgaactggtt cacatcgcgc taccggcgat cgttattact gccgggctct
     7021 ggttcctcgc cggtcctgtt gcttcacttt cagccatttt tgcagtggtg gcaggaaccg
     7081 tactgtttcc tgtcctgctg ccattcattc cgacaaagga cttctccaca aaaggtctcc
     7141 ttctcggggg tatcggttcg atcccgtttg ccggcgcatt ttattctgat acatcccttc
     7201 ccggatgggc caggctcgca tcagctataa ccccgctcct cattatacct gcagttaccg
     7261 catatctatc cctgaatttt accggttcga caacatttac ttctcggaca ggggtcagaa
     7321 aagagatctt ccggtacata ccggttatga cattgatggc ggtctccggg gtaatcctgg
     7381 gcattgcatt aggcttaacc cgccttatgg gggtgatctg atgttcgatt cttatacaga
     7441 aaccaccctt cactatcatc aggacctgtg catcaactgc aaacgctgta cgcaggtctg
     7501 tccccatgga gtgttttctg agggggcgga acatgcggag cttgtccggc cttctgcctg
     7561 catggaatgc ggcgcctgcg ccctgaactg ccccgtccag gcgatcgagg tcaagagcgg
     7621 tgtcgggtgc gcgtgggcga tgattagtgc cgccctcagg ggaaaggata tggacagcgg
     7681 ggaatgcagc tgcggtgggg ctgaggggtc ctgctgtggt gtccaggaag agaagtcctc
     7741 ctgttgccag gataaatgac agcaaaaacg cgggtcctgt ttgtctgcac ggcaaatgcc
     7801 gcccgttcac agatagcaga gggactgtta cgggaaaaat acggggaccg tttcgaagtt
     7861 ttctccgcgg gaacacatct ggcaaaagtg agcgtccggg cgattgaggt aatgagggag
     7921 atcgggatcg atatatcgca ccaccgttca aaaacccttg cagaatattc cggggtatct
     7981 tttgatcttg cagtaacact ctgcgacaat gcccacgcga tttgccctgt tatctatggt
     8041 gcaaaaaaga ccatccaccg tggttttgct gatccccatc tcattccggg tacagatgaa
     8101 gtgatccttg acgggtatcg cagggtgaga aatgaaattg ccagctggat agatgaggaa
     8161 ttcggggatt tctcccaaga ataatcattc ctgtttcctt gcaaccggat ttctgatgag
     8221 atgatggttg ccttgctcac gaacatttaa attacataga ccggctgttg agggtaaaat
     8281 agttacacac gttcctaaac gataaaatag tgaaagtaac agcctatgct cttaggcata
     8341 ttttggcagt tgtgaccggc aaattgacct ttttaacaat tttctttctg gcgtaacaat
     8401 cagtcaggat gtggcgccct gagcacccgg catacctttt ccatgagggt atggtgttgt
     8461 ataagatgat cgataaaagc accgctccga ccttcagcgg cgaccggtgt ccatgctatt
     8521 ccacagtcag tatcccagag gatcagcccc tctgcaggag ccggctggag gggtcgtttc
     8581 gcttctgctt ccagtagccc gctgatagaa ccttcatctg ctttttcttc accgataaga
     8641 attaaagctg atgccataca ccgcacctgg tgccacagga aactttcagc ctgtacttcg
     8701 agaaatacaa atccttcctc atttcccacc cggatagcaa ggatgttccg gtaagggttt
     8761 ttatccttca cccgtgcgaa gttcgaaaaa ttatggctgc caagaaaatt ccgggcagcc
     8821 cggtgcatcg cttcaggact ttttagtttt ctggcaaaat aatacctgta cgtgcgggac
     8881 tttgcatcat agcggggatg aaattccggt tctacgactg ctgacgctgt gcaccagaga
     8941 tctgggggca gctgggtatt gattacagtc tgagcacgtt ccggctcatc ggtggtgaat
     9001 gcagcaacct gcccgcacgc atgcactccg cgatcggtcc ttcctgcaaa ctggaaacct
     9061 gcttcgcgcc aatctgaaaa aagagagagg cgctggcagg cagcaacaaa ctctccttca
     9121 acagtcctct cggaagcctg catctgcgag ccgtaaaacc tgctgcccag atacgatagc
     9181 ctgaacgcca gcctcatcga agccggacgc gccgtgtgaa ccgtttgatt tttctccatg
     9241 tattattcag cgactgatga gtatacgtgc gcaacggggt ctgtctgcca ccacagttta
     9301 ctttgccggc acggatcgca gcaagaattg aagggatatt cctttccgcg tcaataaacg
     9361 ttctgccgta accgacaaat ttcgcatgat gtgcatcact tcccgccaca cagggttttc
     9421 ccagcttcct ggcaatacgt gcagctttcc tgttggcaga accgacgatg tacctgctgt
     9481 tgaacgtttc aatggcatct accacagtca ttcccgcttt cttccggcgc gcaacaccgt
     9541 gccgccagac atgatacggg tgggggagaa ttaaaagtcc ccccagtttc cgtgcaatct
     9601 caaccgtgtc gacaacatcc agtcctgacg ggattatctc tgttatgccc aaaacaatca
     9661 ggtgaccctg ttttgttgag acttcgatac cgggaattac aagaacagtt gtggagcagg
     9721 ataatgcttt ttttgccccg tcaatcgtat catgatcagt gatagcaatt acatcaagcc
     9781 cgacattttc ggccttttta agaatatctt caacactgct ctcaccatct tttgaaaaat
     9841 tggtgtgcac atgcaaatcg cagattagca tctgatcata gtgtttttat ctgtctggta
     9901 ttaagggaac ttatgcgcat cctccttacc acgggtgctg caacagaaga agtcgtcaaa
     9961 tctgcggcgg catctgttga tgcggaggta gtggtgaccg gaaagattgc ttcgtttctt
    10021 accccctaca tgctccacgg gctgatacaa aaaggatcct acgatttggt gattgtcccg
    10081 ggtatgtgta cggcctcctt tgaacaggtt gaacaggaaa ccggcgtccc gatttatcgc
    10141 ggtccccggc atgcagcaga ccttgcattc attcttacca tagtcagcag tgtgaccctc
    10201 tcacgaacca ttcccgctga tgattttctt gcttcgaata aagcacacga cgcccgaaag
    10261 gtgctggaca aacgggaaaa atctgcagaa tgtgatttta ttatccgcgg aacaaagatt
    10321 ggcggcactt cccggatgaa ggtgcttgct gaagttatgg atgctcatct ccacgaggat
    10381 atccgttcaa tcgtggagcg atactttgct gcaggtgctg atatcgttga cctcggtttc
    10441 gggtttgatg cggcccccgc agatgttacg cgggtttttt cccggctgga aggtatcgac
    10501 agaatccttg ctgtggatac ccaggaacct gacctgatac gggcagcact tggccgtgcg
    10561 gatctcgtac tcagccttca ggaacgaaat attcctgagg tgggagaagc gatagcaaaa
    10621 gttggcgcag cggcggttgt agttccgggg gaaaattctc tcaaaaagaa cctcaccctt
    10681 gccaaaagag ccggtatctc ctgtataatt gccgatcctc tactccagcc ggtcggatcc
    10741 ggtcttgtcg cttctttgaa aaatttcaaa aaatacggtt ttccgctttt ctttggtgcg
    10801 ggaaatgtca cggaactgat cgatgccgat tcaatcggga taaatgcgct ccttgcgggg
    10861 atggcgatgg agactggtgc agcagtgatc ttcaccagcg agcattccga caagactaaa
    10921 gggtccgtca gcgagatgcg gaaggcaacg gagatgatgg cactttcagc aaaccgtccg
    10981 tatcctaaag atctcgggat cgatctgctg gtgctcaagg agaagcgccg tcgccgtgaa
    11041 ccaccgcttg aatacgatac cgccatacct gcaattacga tgccagacag gattgaatat
    11101 gacccaaaag gtaacttcag gataggaatt gaaggtgacc agatcgtggc ggtgatcaat
    11161 gacagggcga taagggggaa gcactggcag gatgtcctgc acacgatcct ttcccacgac
    11221 aatgtctctc ttctcgacca tgccgggtat ctgggcaggg agttatacaa ggcagagctc
    11281 gccatacggt tcgggcggag tttcgagcag gacggggagt tttaaaaaaa actagggcaa
    11341 ttcggagatg aaaaattgtg cggctgaaaa actaaaggat gtattcctct taatgaactc
    11401 ttttattcca tcccccctca tttctatata tggctgtaca ggcgcgtgca tcacatattc
    11461 tcgtcaagac tgaagaccag gcaaaccagg tgctcaaacg acttgccgac ggcgaagagt
    11521 tcgacaaggt cgcccgcagg ttctcttcct gcccctcggg aaaaaacggg ggggatcttg
    11581 ggtggtttgg caaaggacag atggtaccgg aatttgagaa agtggcattc gaaaacgaag
    11641 ccggtaaggt tgttggcccg gtcaagacgc agttcggtta ccacgtcata aaaattaccg
    11701 gcaggaaata agttctccgg cttcggctca tggatacaaa atttttcctt ggcataggtc
    11761 ttgcgatagt acttgtcttc tgcagtgcag cggtctactc aagttttacc agatttccaa
    11821 ccgcatcggc gatacctgaa ggtcaggaga tcctctacca ggtatcgacg atcgatgcac
    11881 tgatgcaggg agtgtacgat ggtgtccagc cggtcgggga gataaaaaag cacggggact
    11941 ttgggatcgg cacgttcgat gcgcttgaag gcgagatgat cgtgctcgac ggcgtggtat
    12001 accaggcaaa atctgatggt gtgatatatt cggtggcgga caacaccacc actccttttg
    12061 ccacggtgac atacttcgcc cctgacttca atacgactac cggccgacta atgaactttt
    12121 ctgagttctc gacggagatg aacacccgtt taccctcgca gaatatgatc tacgcggtac
    12181 ggatgaaggg gacctttccc cacatgaagg ttcgggcgat acctgcacaa cagaaaccct
    12241 atcccacact cacggatgcg gcaaagaacc agtcggttta tacgtatgat gatgcccgcg
    12301 gtaccgttgt cgggttttat accccggtct ttttcaaagg aatcaatgtc gtgggctacc
    12361 atctccattt catcagcgat gaccggcatt ccgggggtca catcctcgat tttgaggtgc
    12421 cggtcaacac ccgggtggag tacgacatca caccggaatt taagatgctg cttccgacga
    12481 gtggagcgtt tatagaagtc gatctttcaa aggatctgag tcagggactt gcacgggtgg
    12541 aatcgtgatt tttgtttttt accatcattt ttttgagagc attttttgac aagcagcact
    12601 catataaccg gtacatcgcc cgaaaagcgt gccaggttcc cgaccatgaa catccgagga
    12661 attaaacata atcttctcaa gctcctttta gaactgggaa gggactccca ccccaacgag
    12721 ttcgtggcac ttctccggga gaataacgga gttatcgacg agctga
//
LOCUS       DASRLV010000050         6850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_988223,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000050 DASRLV010000000
VERSION     DASRLV010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 6850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6850
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988223"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..674
                     /locus_tag="VFC43_02145"
     CDS             84..674
                     /locus_tag="VFC43_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15e"
                     /protein_id="HZK30021.1"
                     /translation="MVKSMYAFVREAWKVPEKSGVKALLWNRMQEWRREGSVVRLEHP
                     TRIDRARSLGYKAKQGVAVARVQVRRGGRRASRYVRARRSARMGKNRSTAGKSIQRIA
                     EERASKRFPNMEVLNSYWVGQDGKLKYYEVILVDGHHPSVRADKNLAWLANATHRGRA
                     ERGKTSAGRKGRGMSTRGKGTEKTRPSIRSHANQGK"
     gene            707..1348
                     /locus_tag="VFC43_02150"
     CDS             707..1348
                     /locus_tag="VFC43_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P subunit p30 family protein"
                     /protein_id="HZK30022.1"
                     /translation="MKITDACVCPYPCGDSSVRRMALEARDLGFERIVAKDVPSGEVY
                     GIELYTGILIRDMPLRDLVNRIRREKNSHAVVSVQAGDSGFNRAVITTKGVHILRGIQ
                     SADRHAFDHVTARMAADNRVAVDIDLSPMISGRGIARQRAIHRYRDIMVLEQRFGFPL
                     VISSHARSLLEMRSVREITGLCSLIGMDIPDVEKAFSGIDTVTTPPDAAVRVI"
     gene            1348..1842
                     /locus_tag="VFC43_02155"
     CDS             1348..1842
                     /locus_tag="VFC43_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpp14/Pop5 family protein"
                     /protein_id="HZK30023.1"
                     /translation="MGTRPPTLREKRRYLLVALEPAGTAVDQKDLYYALSDATTQLWG
                     DATAAVITPAVVASEGQFFIIRCRRGTEHELAIALSTITTCRDTRIALRMVAASGTIE
                     SLRERIRCRMSPGPDQLAPGECIFDKKTFQLSQCNGQKVDVIEKGFKNTNRLFLTRED
                     MEEL"
     gene            1842..2564
                     /gene="psmA"
                     /locus_tag="VFC43_02160"
     CDS             1842..2564
                     /gene="psmA"
                     /locus_tag="VFC43_02160"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit alpha"
                     /protein_id="HZK30024.1"
                     /translation="MQPQYQMGYDRAITVFSPDGRLYQVEYAREAVKRGTTAVGIKAK
                     DGVVLIVDKRVSSKLLEASSIEKIFKIDEHIGVASSGLVGDARSLVDRARVECQINRV
                     SYDDPIEVETLAKKLCDHMQTLTQYGGIRPYGTALLIAGVSDGESRLFETDPSGTLLE
                     YKATGIGIGRPAAMKVFEEEYNPESEIKDVILLGLKALHSATEGKFDVDTVEIGVIEK
                     ANPVFRKMTKEEVATFVGQFKQ"
     gene            2571..3275
                     /locus_tag="VFC43_02165"
     CDS             2571..3275
                     /locus_tag="VFC43_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly factor SBDS"
                     /protein_id="HZK30025.1"
                     /translation="MIPLDKAVVARLESFGERFEILVDPNQAALVRQGQKVDIEDVVA
                     ALNVFGNASRGTRASDEALEKVFHTTDFNTVARRIIEKGEIHLTSEQRKHMIEEKRRQ
                     VITFIARNAVNPQTGHPHPPHRIEMAMDEARVNIDPFKHLDEQVKETVKALRPLLPIR
                     FEELRLAIRIPADFAAKAYGDIAAGSTMEKDEWQKDGSWVCVVRIPAGIQGEFYDLVN
                     KLSKGEGQVKILNQVY"
     gene            3319..3612
                     /locus_tag="VFC43_02170"
     CDS             3319..3612
                     /locus_tag="VFC43_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37ae"
                     /protein_id="HZK30026.1"
                     /translation="MASSTHKAKGKVTGSAGRFGCRYGRFVRKRVADIEAISGALHRC
                     PKCDMQSVRRKGTGIWECRKCNYKFAGGAYQPQTPAMKIAQRAIDRTLEQIKG"
     gene            3622..3756
                     /locus_tag="VFC43_02175"
     CDS             3622..3756
                     /locus_tag="VFC43_02175"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit P"
                     /protein_id="HZK30027.1"
                     /translation="MASTYKCARCKQKVEIDINVRCPYCGHRILFKERGAAIKEIKAR
                     "
     gene            3834..4085
                     /locus_tag="VFC43_02180"
     CDS             3834..4085
                     /locus_tag="VFC43_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HZK30028.1"
                     /translation="MTLHEALFRFSTPYAGQIYQALIPELTDEVNSRSVTRCWTEGDS
                     MLILCVEAQDIPALRAALNMALRLVNVADEMQDISRPTR"
     gene            4208..4570
                     /locus_tag="VFC43_02185"
     CDS             4208..4570
                     /locus_tag="VFC43_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit beta"
                     /protein_id="HZK30029.1"
                     /translation="MNNLSPKVQNQLSMLQQIQQQLQTVLSQKAQYEMAVREAKRAQE
                     EIADSAEDAVMFMSVGTVMMQKKKEVVNAKLTEKVETLELRVKSLEKQEKMMQGKFEQ
                     LQAQIKAAIEGKSAPNAA"
     gene            4785..5381
                     /locus_tag="VFC43_02190"
     CDS             4785..5381
                     /locus_tag="VFC43_02190"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZK30030.1"
                     /translation="MGTRPVVLEQDPVLRMAVIKIAADHASQAHKNQLRKDRKTPYIT
                     HPARVAALVGMFNSSHVAIVSAWLHDVYEDCTPEWIRRTDEFIKGLPLSGDEKNDITA
                     IVGALTKKNTIKGKAARLSNSLDRILDAPPEATLVKLCDRIDNLLDSADRNGGFTKRY
                     LASTDEVIGKLEVRSSLYGYEAALNLLIEIRNAKRKKE"
     gene            complement(5451..6850)
                     /locus_tag="VFC43_02195"
                     /pseudo
     CDS             complement(5451..6850)
                     /locus_tag="VFC43_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107382.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
BASE COUNT         1785 a         1725 c         1840 g         1500 t
ORIGIN      
        1 ccggttaaag tatgtgggaa tacgggatgc gtcccgttga aaggaatgta gctcaaaaat
       61 gagccgaacc ttaggtgatt atcatggtaa aatcaatgta cgccttcgtc agggaggcat
      121 ggaaagtacc ggaaaaaagc ggagtgaagg cacttctctg gaaccgtatg caggaatggc
      181 gccgtgaagg cagcgtcgtc cgactcgagc atcccacccg cattgaccgc gcgcggtcac
      241 tgggttacaa ggcaaaacag ggtgttgcgg ttgcacgggt acaggtacgc cgcggtggtc
      301 gcagggcatc aaggtatgtc cgcgcacgac ggtcggcacg catgggaaag aaccggtcta
      361 cggcaggaaa gagtatccag cgaatagccg aagagcgggc ttcgaaacgc ttccccaaca
      421 tggaagtgct caattcctac tgggtcgggc aggacggcaa gctgaaatat tatgaagtca
      481 tccttgtcga tggtcaccac ccctcggtga gggccgataa gaaccttgca tggctcgcaa
      541 atgctacaca ccgtggcagg gccgagcgtg gcaagacctc agccggacgc aaaggtcggg
      601 gcatgagcac ccgtgggaaa gggacggaaa agacccgccc gagtatacgg tctcacgcaa
      661 accagggcaa ataatcttat cctcttttta cgcaaagtta cctttcatga agatcaccga
      721 tgcctgtgtc tgtccatacc cgtgcgggga ctcatcggtg cgccggatgg cacttgaagc
      781 cagagatttg ggttttgaac gaattgttgc aaaagacgtg ccttccggcg aagtatacgg
      841 gatcgagctg tataccggta ttcttatccg tgatatgcca ctgcgggacc tggtcaaccg
      901 catcaggcgg gagaagaata gtcacgcagt cgtatcggtc caggccggtg acagcgggtt
      961 caaccgggca gtgatcacca caaagggggt gcacatcctc aggggaatcc agtctgcaga
     1021 taggcatgca ttcgatcatg taaccgcccg gatggcggct gataaccggg tggctgttga
     1081 catcgacctg tcccccatga tatccggaag agggatcgca cgccagcgcg ccatacatcg
     1141 ttaccgcgat attatggtac ttgagcaacg gttcgggttc ccgcttgtca tctcatccca
     1201 tgcccgatca ttactggaaa tgcgttccgt ccgcgagatt accgggctct gctcccttat
     1261 cggcatggac atacctgatg ttgaaaaggc gttttccggt atcgatacgg tcaccacccc
     1321 gccggatgcg gcagtcaggg tgatctgatg ggtacccgtc cccccacgtt gcgggagaag
     1381 cgccggtatc tcctggttgc tctcgaacct gccggaaccg cagtcgatca aaaggactta
     1441 tactacgcgc tatcggacgc cacaacccag ctctgggggg atgcaacggc agcagtcatc
     1501 acaccggcag tcgttgcttc cgaagggcag ttctttatca tacggtgccg gcggggaacg
     1561 gagcatgaac tggcaatcgc cctctccacg attaccacct gcagggacac ccgcattgcg
     1621 ctgcgtatgg ttgcggcttc gggtacaatt gaaagcctgc gcgaaaggat acggtgtaga
     1681 atgagccccg gaccggatca gctggctcct ggcgaatgta tctttgataa aaaaacgttc
     1741 cagctcagcc agtgtaacgg tcagaaggtt gatgtgattg aaaagggatt taaaaataca
     1801 aaccgattgt tcttgacaag agaagatatg gaggaattat aatgcaacca caatatcaga
     1861 tgggatacga cagggcgatt accgtctttt ctcccgatgg caggctttac caggtggagt
     1921 acgcccgtga ggcagtcaag cgtggaacaa ctgccgtggg tatcaaggca aaggacggcg
     1981 tcgtgctgat tgttgataaa cgcgtgagtt cgaaactcct cgaggcatca tccatcgaga
     2041 aaatttttaa aattgatgaa catatcgggg tcgcatcatc cggtcttgta ggagatgcgc
     2101 gatcgcttgt tgaccgtgca cgggtagaat gccagatcaa ccgggtctca tacgacgatc
     2161 cgattgaagt cgaaacactg gcaaaaaagc tctgcgacca catgcagacg ctcacgcaat
     2221 atggcggtat tcgcccgtac gggacggccc tgctcattgc aggagtaagt gatggtgaat
     2281 cccggctttt tgaaacggac ccgtcaggga cactcctgga atacaaagca accggtatcg
     2341 gtatcggacg ccccgctgca atgaaggttt ttgaagagga atacaatccc gaatccgaga
     2401 tcaaggatgt aatcctcctc gggctcaagg ccctgcattc cgcaaccgag ggcaagttcg
     2461 atgtggacac cgtggaaatc ggggtaattg aaaaagcgaa cccggtattc cgtaagatga
     2521 caaaagaaga agttgcgacg ttcgttggac aattcaagca gtgaattccc atgataccgc
     2581 tcgataaggc cgtggtagca cgccttgaga gtttcggaga gcgctttgaa attctcgtag
     2641 acccgaacca agcagcactg gtacggcagg gccagaaagt cgatatcgaa gacgtagttg
     2701 ccgcactgaa tgtctttggc aacgcgtcac ggggcacgcg ggcatcagat gaagcactcg
     2761 aaaaggtatt ccataccacg gattttaaca cggtagcgcg gcggattata gaaaaaggtg
     2821 agatccatct cacttccgag caacgcaaac atatgatcga ggaaaagcgc cgtcaggtta
     2881 ttaccttcat tgcaaggaac gccgtcaacc cccagaccgg ccaccctcat ccaccccacc
     2941 gcatagaaat ggcaatggat gaagcgaggg ttaacatcga cccgttcaag caccttgatg
     3001 aacaggtcaa agaaacagtc aaggcgttac gaccgcttct tcccatccgg tttgaagagc
     3061 tgcgcctagc aattcgcatc ccggcggatt ttgccgcaaa agcctacggg gatatcgctg
     3121 ccggatccac gatggagaag gacgagtggc agaaagatgg ttcatgggtt tgtgtagtcc
     3181 ggatacctgc cggaatacag ggagaattct acgatctggt caacaaactc tcaaaaggag
     3241 aagggcaggt aaaaatcctc aatcaagtat attagttacg aggacaaata aaagaagaca
     3301 gatcggtggg aatgcattat ggcaagctcg actcataaag caaaaggaaa agtaacaggt
     3361 agtgccggac gttttggctg ccggtacgga agatttgtac gcaagagagt agctgatatc
     3421 gaagcaatat caggcgccct ccaccgctgc ccgaaatgcg acatgcagtc ggtgaggcgg
     3481 aagggcaccg gtatctggga gtgccgcaag tgcaattaca agtttgccgg tggggcatac
     3541 cagccgcaga cgccagcaat gaagattgcc cagcgcgcga ttgaccggac tctcgaacag
     3601 ataaaaggat aatttttgca tatggcaagc acgtataaat gcgcacggtg taaacagaaa
     3661 gtcgagatcg acatcaatgt ccggtgccct tactgcgggc acagaattct ctttaaagag
     3721 cgtggcgccg caattaaaga gattaaagcc cgttaattgt tcatccggga cacgtgcggg
     3781 aaaagaatag tttatcatct ttttttatcc atataccttc acgaggttgt cctgtgaccc
     3841 ttcacgaagc actcttccgg ttttcaactc catatgcagg acagatttac caggctttaa
     3901 tccccgaact taccgacgaa gtcaattccc gctcagtgac ccggtgctgg actgaaggtg
     3961 attccatgct catcctttgc gtagaggctc aggatattcc ggcactccgg gccgcgctca
     4021 acatggcgct gcgcctggta aatgttgccg atgagatgca ggatatttcc cggcccactc
     4081 gataaacaag tcgagatctc cgatacatgc caggccattc gaacaggaat aaccgggcgg
     4141 cgaaaggaat tgtaagaata atccttttta tcaattggag ccgattacaa taacaggagt
     4201 tatacaaatg aacaatcttt cacccaaagt ccagaaccag ctgtccatgc tccagcagat
     4261 acagcaacag ctccagacag tcctctcgca aaaagcccaa tacgagatgg ccgtgcgtga
     4321 agcaaagcgt gcccaggaag agatcgccga ttctgcagaa gatgccgtca tgtttatgag
     4381 tgtcggaaca gtcatgatgc agaagaaaaa agaagtcgtc aatgcaaaac tcactgaaaa
     4441 ggtcgaaacc cttgagcttc gcgtaaaatc cctggaaaaa caggaaaaga tgatgcaggg
     4501 aaaatttgag cagttacaag cacagatcaa ggcggcaatc gagggcaaaa gcgccccgaa
     4561 tgcagcttaa tcattttctt attcagcctt tttaagcaaa ccagattaaa actgataggt
     4621 attttctatc agtcatagta tgaccggaag ggtattcttc tgatatgagt ctttacacgt
     4681 ccgatggtta tttttccatc tgaccgctct gcctgtgcca ggattggatg aaccgggatc
     4741 caaaaacaaa aatcctttag gttgcctcat gcagagtacc tggtatgggt accagaccgg
     4801 ttgtattgga gcaggatccg gtcttgcgaa tggcggttat caaaatcgca gcggaccatg
     4861 cttcgcaggc gcataaaaat caattgcgta aggaccggaa gacaccctat atcacccacc
     4921 ctgcaagggt agccgccctc gtcgggatgt tcaacagttc ccatgtggcg atcgtctcgg
     4981 cgtggctcca tgacgtgtat gaggactgca ctcctgaatg gatcaggcgg acagacgagt
     5041 ttattaaagg acttccgctg tccggcgatg aaaaaaatga catcacggct attgtcggtg
     5101 cccttaccaa aaagaatacg atcaagggaa aggcagcccg gctctccaac agtcttgacc
     5161 ggatccttga cgctcccccc gaagcaacgc tggtgaaact ctgtgaccga atcgacaatc
     5221 ttcttgattc ggcggatcgc aacgggggtt ttacaaagcg gtaccttgct tcaaccgatg
     5281 aagtcatcgg aaaactcgag gtacggtctt ctctgtatgg gtacgaggcg gcgctgaacc
     5341 tgcttatcga gatccgaaat gcgaagcgga aaaaggagta aagacaagtt ctggagaagg
     5401 taaaaaatta cccgctccac ttaaaaaaaa cagggaataa atccgtggac ttatttccgg
     5461 ttaatcaggt tgattgcatt gaccagtgcc tcgacggatg agaggacaat gtcatcgccg
     5521 ctgccgcttg catcaaaaat tttcccgtgt tcgtcttcta cggcaatggt gacatggcac
     5581 atcgcatcgg aaccgcctga tattgcctcg atattgaatt ccttgagctg gaggcgggcg
     5641 ggcgtgatcc cgaggatcgc atgaagtgcg gcatcaaccg ggccattgcc cacgctagaa
     5701 aagacgtgct ctttcccgtt tacggtggct ttcacgcttg cggtcgggat cacatggttg
     5761 cccgtcataa tggcaatatc gaccagctcg aacatcttgt ggttgagttc gattcccatc
     5821 acgctttcgg cgatctcgta gagatcagca tccgtcacgc gttttccctt cccggcaatc
     5881 ccttttacct tttccacgat catatccagc tgcgggtcag aaggttcgat atggacatct
     5941 gcaagcatct ggaacaccgc gtgtcttccg acgtgttttc caagggtcag ccgcctgcgg
     6001 tgcccgacca tttccggagt catgatgccg ggttcgaatg ttgcggtatt ttttataaca
     6061 ccgtgcgaat ggatgccact ctcgtgggag aatacatttt caccaacaac cggctgggtg
     6121 ggagtaatgc tgatgcccga gaaccgggag accagccgcg acgtttcaac cagccgttcc
     6181 ttttgaatgc ctgtcctgat gccgtatatt gcctccatga tcataacggt ctgggcaaga
     6241 tcggcattgc ctgcacgttc accaagcccg ttcacggtaa cctgcacctg ggaagcacct
     6301 gctctacagc ggcgatcgtg tttgctacgg caaggccaaa gtcgttatgg cagtggacgt
     6361 cgattggaca gctgactttt ctggcaatac tggagacaag ggccttcata ccggaagggg
     6421 agataacccc aaccgtatcc ggcacattga tgatagtagc cccggcatct gcagccgtcc
     6481 ggaaaacttc tatcaggtag tccccgtcag tccttgtcgc atccattgcg gagaacatgc
     6541 agctgtccgt atggtcgcgg atatagtgaa taatatcttc agtaatcccg agcacttcct
     6601 cctgcgtctt gttgatcgtg ttctcccgct ggatatctga tgtagggata aagacgtgga
     6661 ccatatccac gttgcagtca aggcaggcat caacatcggc ttttacggaa cgggcgagcc
     6721 cgcagatctt cgcatcaaga ccgagctttt taatagcggt cacggtctct ttctcgactt
     6781 ttgaggatgc aggaaaccct gcttcgatta catggatgcc gatatcagag agctgacgtg
     6841 cgatctcgag
//
LOCUS       DASRLV010000051         7993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_418386,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000051 DASRLV010000000
VERSION     DASRLV010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7993
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418386"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1012
                     /locus_tag="VFC43_02200"
     CDS             71..1012
                     /locus_tag="VFC43_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HZK30031.1"
                     /translation="MDFISIILIIAGLVLFETITSIDNAIINAEVLSTMGERARRWFL
                     IWGIIFAVFAIRGLLPWLIVWLSTPSLSPLGAFTATFSSDPIVLAAIEQSAPILLMGG
                     GTFLLFLFLNWLFLEQKNFGLRGERYIASKGVWFFAIVSVLLTLLVWFSLEKGPLIAF
                     GAVVGSTAFFIVHGFRQNAEKAEEKMLHSSGMSDLSKIFYLEVIDATFSIDGVIGAFA
                     FTMAVPLILLGNGIGAFVVRELTIRNVDNIKKYRYLKNGAMYSIFFLGIIMIFDSFGY
                     HIPSWVSPIITFGVVGYFLMKSLKEIPRVPKEDKNPG"
     gene            complement(1047..2288)
                     /locus_tag="VFC43_02205"
     CDS             complement(1047..2288)
                     /locus_tag="VFC43_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZK30032.1"
                     /translation="MLFRTVPKTGDSLSILGFGFMRLPLKNGGGIDEERAILQLRHAI
                     DRGVNYVDTAPAYHFGKSEKILARALADGYRKKVRIATKLPPWSVHSRDDMIRILSSQ
                     LTTLRTDHIDYYLLHSLTKASWEKLRSLGVLDFLDDAKKHGLIMNAGFSFHDDTETFK
                     EIVDAYDWDFCQIQYNYLDEHNQAGTEGLAYAAGKRLAVIIMEPLRGGNLAGNVPDQI
                     KKIWNEGAKKRSPAEWALRWVWNHPEVTVVLSGMNDESHIEENLRIASDAFPGSLSPS
                     EMEIISRVRDSYQRLMKIACTGCGYCMPCPAGVDIPGCFSLYNAHHLFPHDRSAKFHY
                     FGRHGGLLSGVSYAGMCRQCKKCMKSCPQHLQIPDRLRDVSKEMEGMMTVIVPVLKGG
                     LWCMDKIGRVGRAFSLHKILK"
     gene            complement(2424..3230)
                     /locus_tag="VFC43_02210"
     CDS             complement(2424..3230)
                     /locus_tag="VFC43_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZK30033.1"
                     /translation="MSLALKQKIITTCAELDIPLVGFAPARRWDEPLFEPWIPEEFRP
                     QSIFPETRTVIVIGLPVSLPILETAPSIYYHELYRTVNTHLDVSGYRISLILNSLQLP
                     SIWIPRDGYGSISILKERPLAFFSHRHAAFLAGLGNFGINNMLLTEKYGPRVRFASIF
                     TAADIPPDPVIQNPLCTSCMLCVNSCPVKALDGRKYPKGLTDKKACAIRSEALSKRYI
                     SPCGLCIKVCPVGGDRKLYAREDMRMYTGDSMEYEKYHKAWKHVRSYGGR"
     gene            complement(3298..3708)
                     /locus_tag="VFC43_02215"
     CDS             complement(3298..3708)
                     /locus_tag="VFC43_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HZK30034.1"
                     /translation="MFQTTFSVSTQREGDIIDITSHVQKAVRDSNIQQGIIHLFVQHS
                     TAALTTIEFEPGVLEDLKRALSVLAPDNAEYAHNARWGDGNGRSHVKAALIGPSLTIP
                     VDEGTLKCGTWQQIVLLELDVNAGRERKVVCSVF"
     gene            complement(3878..6397)
                     /gene="gyrA"
                     /locus_tag="VFC43_02220"
     CDS             complement(3878..6397)
                     /gene="gyrA"
                     /locus_tag="VFC43_02220"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HZK30035.1"
                     /translation="MTSEVPPASGPETHRTEPISIAHEMKTSYINYAMSVIIGRAIPD
                     VRDGLKPVHRRSLFAMWEMGNTSDKPTKKSARVVGDVMGKFHPHGDASIYDTIVKMAQ
                     PFSYRNMLVQGQGNFGSVDGDSAAASRYTEVRLFPYAEALLEDLDKDTVEFVPNYDES
                     LKEPTVLPSKIPNLLVNGTDGIAVGMATKMPPHNLREVCAAVNLYLDNPEIPVEDLMK
                     VMPGPDFPTGGIMMGTEGVRNIYTTGQGRVIIRGVATIEESEGGNRGDRIIVSELPYQ
                     VNKAQWITAIAEMVKDKRIDGLSDIRDESDKDGIRVVFELRKGTISAVILNQLYKLTA
                     LESSFWAINLAIVDNQPRVLTLPGLLENFVQHRIEVIRRRSEFDLKKAQDKVHILVGL
                     LMALSKIDLIIAAIRASDSVDNARMALITQFGLDELQANAILQMQLRRLAALEQQKIN
                     DEKKGLEAEIGRLETILSNEANIKDEIRRETVEIAAKFGDARRTEIAIDTSDLSNEDL
                     IEDKTVLISLTTANYIKRMDIDTYRKQRRGGHGITGMTTKDDDGVDRVFAAEMKDYLL
                     CFTNIGRVYWLKVYQIPESSRIAKGKPIVNLLNLKDEIVTKVIPIRVFREDRFMLFAT
                     RYGQVIKIPQTEFSNPRSVGTNAIRLKENDQLVDVIATDGSKEIVLSTRFGYSLRFHE
                     ETVRTVMRNASGVRGMKLRGEDTIVALTLVEKDHLLTISDVGFGKRTEFDDFRGHGRG
                     TMGVRNMQLERNAIVIESRAVLDTDEIIVMTASGVVIRMPVSEIRVIGRGTKGVRTIR
                     LDDKDRVVGVAIVQPEIEVVPETNNGPEPENTTSSNNDTGE"
     gene            complement(6387..>7993)
                     /locus_tag="VFC43_02225"
     CDS             complement(6387..>7993)
                     /locus_tag="VFC43_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HZK30036.1"
                     /translation="SKGLMTSPLSTREESLNELLARYQQWYGAPAPFSRTSEVIGPAE
                     SEHESRAAFLAQFGPKMTGTRVHFVPDATIFETTTFDYDVLAHRMRELAFLNAGLTIT
                     ITDERSSDTATYCYAGGLIEFVKYLNEGVECLHPFPIDISKKDIENKLEIEVAMQYTT
                     AYDEKIYSYVNSVNTRDGGTHLEGFRSAITRTINTVAKRNGLIKENSAITLRGEDVRE
                     GLTSVVSIKMANPQFEGQTKMRLGNSSVKGTVDSLVYAAMSEYFDENPNVLKIIIEKA
                     LSAAKAREAARNARDLARRKSSLESSGLPGKLADCSERDPAKSEVYIVEGDSAGGSAK
                     QGRDRKFQAILPLRGKILNVEKAGEHQILKNAEIQTLISAIGTGIGEKFDQERARYHH
                     IVIMTDADVDGAHIRTLLLTFFYRYMLKLVEAGYIYIAQPPLFRISKGKEEKWVYTEE
                     EMKTASAAMGEKGVSVQRYKGLGEMNAQQLWDTTMDPEYRVFKQVTIEDALEADEMFR
                     TLMGKDVEIRKNFIIRHAKEVTNLDI"
BASE COUNT         1765 a         2050 c         1999 g         2179 t
ORIGIN      
        1 ataatccttt atttcaccgg catcttccgc atatattttt ttacaaacaa tgcttatagg
       61 tagtatactc atggatttta tttccattat ccttatcatt gccggcctgg tcttattcga
      121 gaccatcacg agcatcgata acgccattat taatgctgaa gtgctttcca ccatgggtga
      181 gagggcacga cgctggtttt tgatctgggg aatcatcttt gctgtctttg cgatccgggg
      241 gctgctaccc tggctcattg tgtggttatc cacaccctca ttaagtcctc tcggggcgtt
      301 caccgcgaca ttctcaagtg acccgatagt ccttgcagct attgaacagt ctgcccctat
      361 tctcctgatg gggggcggga cattcctgct gttccttttc cttaactggc tgttcctcga
      421 acagaaaaat tttggcctca gaggagaacg ctatatcgct tcaaaaggtg tctggttctt
      481 tgcgattgta tctgtcctgc tcaccctcct tgtctggttc tctcttgaaa aaggtccgct
      541 cattgcattc ggggcagtag ttggttccac cgcgtttttt atcgttcacg ggttccggca
      601 gaatgctgaa aaggctgagg agaagatgct tcattcatca ggtatgtcgg acctgagcaa
      661 gatattttat cttgaagtca tcgatgccac attctccata gacggggtta tcggggcatt
      721 tgcattcacg atggctgtcc ccctgatact ccttggtaat ggtatcggag cgtttgttgt
      781 ccgggagctg actatacgca atgttgacaa tatcaagaaa taccggtatc tgaaaaacgg
      841 ggcaatgtat tcgatattct ttttgggaat tatcatgatt tttgatagtt tcggttatca
      901 cattccctcc tgggtctcac caattataac cttcggcgtt gtcgggtatt tcttaatgaa
      961 atccctaaaa gagatccccc gtgtcccaaa agaggataag aaccctggtt aatttcccct
     1021 cctttctctg cagataaaaa gtgggattat ttcagaattt tatgcaggga aaatgcacgc
     1081 cccacccgcc cgattttatc catgcaccag agtccgcctt tgaggacggg aacaatgact
     1141 gtcatcatcc cttccatttc ttttgagacg tccctgagcc ggtcgggaat ttgcaggtgc
     1201 tgagggcagc tcttcataca ttttttacac tgccggcaca tcccggcata ggaaacaccg
     1261 gaaagcagtc caccgtggcg cccgaaataa tgaaattttg cgctccggtc gtgagggaac
     1321 aggtggtgtg cgttatacag ggaaaaacaa cccgggatgt ccactccggc cgggcagggc
     1381 atacaatacc cgcatccggt acaggctatt ttcatcaggc gctgatagga atcccttacc
     1441 cgtgaaataa tttccatctc cgatggagac agggaacccg ggaacgcatc gctggctatc
     1501 ctcaggttct cttctatatg ggattcgtca ttcattcccg aaagcacgac cgttacttcg
     1561 gggtggttcc atacccagcg gagcgcccat tcggccgggg atcgtttttt ggcaccttca
     1621 ttccagatct tttttatctg gtcagggaca tttccggcaa ggttgcctcc ccgtagcggt
     1681 tccatgatga tgacagccag gcgttttccg gctgcatacg caagtccttc cgtacctgcc
     1741 tggttgtgct catcgaggta attgtattgg atctggcaaa aatcccaatc atatgcatca
     1801 acaatttctt taaacgtctc agtatcgtca tggaatgaga acccggcatt cattatcagg
     1861 ccatgctttt tcgcatcatc caggaaatcc agaacaccga ggctcctgag tttttcccag
     1921 ctcgcttttg tcaggctgtg gagcaggtaa taatcaatat gatctgtccg gagggttgtt
     1981 aactgtgagg aaagaatacg gatcatgtca tcacgtgaat ggacagacca tggcgggagt
     2041 tttgtcgcaa tcctgacttt tttgcggtac ccgtctgcga gtgcccgtgc aagtattttc
     2101 tcacttttac cgaaatgata tgcgggagcc gtatcaacat aattaactcc cctgtcgatc
     2161 gcatgccgca attgcaagat ggcgcgttct tcatcaatac cccctccgtt tttcaatggc
     2221 agacgcataa atccgaaccc gaggatcgag agtgaatcac cggtttttgg aactgtacga
     2281 aagagcatgg ggacacattc tttgacgctt gctttgatta atcaggagaa ttggtactgg
     2341 cgctataaaa tttttcttga gaggatgtat tagatagcca cggagagcac ggaaatacga
     2401 tttttggatg taccggattg cggttacctc cctccgtatg atctgacatg tttccatgct
     2461 ttgtgatatt tttcatattc catggaatct ccggtataca tcctcatatc ttcccgtgca
     2521 tacaatttcc tgtccccgcc aaccggacag actttgatac acagtccgca gggtgagatg
     2581 taccgtttgg ataaggcttc tgatcggatt gcacatgctt tcttgtcagt aagccctttg
     2641 ggatatttcc tgccatcgag ggctttcacc gggcaggagt tcacgcatag catgcatgac
     2701 gtgcataagg gattctgaat aacgggatcc gggggaatgt ctgctgcggt gaaaattgag
     2761 gcaaaacgga ctcttggtcc atatttttca gtcaggagca tgttattgat cccaaaattc
     2821 cccagacctg caagaaatgc agcatggcgg tgagagaaaa aagcgagtgg tcgttccttg
     2881 aggatgctta tggatccata cccgtcccgg ggtatccata ttgagggcaa ctgcagggag
     2941 ttcaggatca gggaaatcct gtacccgctt acatccaggt gggtatttac tgtccggtaa
     3001 agttcatgat aatagatcga tggcgcagtt tccagaatcg gcaggctgac cggaagacct
     3061 ataacgatga ccgtacgggt ttccgggaaa atcgactgtg gccggaactc ttcaggaatc
     3121 cacggctcaa acaatggttc gtcccatctc ctggcaggtg caaagcccac aagaggaata
     3181 tccagttccg cgcacgtggt gatgattttt tgtttcaggg ccaggctcat acgttaggat
     3241 agcacggacg gtgttatatg gatttggaag gggtgtgcat taagaacggt tcattatcta
     3301 aaaaaccgag cacacaacct tccgttccct tcccgcattc acatccagct caagaagaac
     3361 aatctgctgc catgtcccgc atttcagggt accctcatca acaggaattg taagagaggg
     3421 gccaataagg gccgctttga cgtgtgaacg cccgtttccg tcaccccacc gggcgttatg
     3481 cgcatattca gcattgtctg gtgcaagcac agaaagtgcc cttttcaggt cctcaagaac
     3541 acccggttca aactcgattg tcgtgagagc cgctgttgag tgctgaacaa agagatggat
     3601 aatcccctgc tgtatgttgc tgtccctgac tgctttttgc acatgcgatg tgatatcgat
     3661 gatatcgcct tcgcgttgcg tgctgacaga aaatgttgtc tgaaacatca gttcaccgta
     3721 tactcaactc ttttgtgctc ctgcccaaaa attattcctg ataatcgatc acacgatcac
     3781 ggtacgattt tttgtcggca gaaataactc ttttccgtaa atgtacaata cttcaaaaac
     3841 ggggccaaaa aaaaaagata cgctgcaggt tctgaattta ttcacctgta tcattattac
     3901 tgcttgtggt attttccggt tctggtccgt tatttgtctc gggaacgact tcaatttcgg
     3961 gctggacaat cgccacgccc acaacccggt ctttgtcatc caggcgtatt gtcctgacgc
     4021 cctttgtccc ccgcccgatg acccgaatct cactgaccgg catacggatc acaacaccgg
     4081 atgcggtcat gacaataatc tcatcggtat ccagtactgc acgcgactcg atgacgattg
     4141 cgttccgttc gagctgcatg ttcctgacac ccatcgtgcc ccggccatga ccgcggaagt
     4201 cgtcaaattc cgtccttttg ccaaacccaa cgtcggatat ggtaaggagg tgatctttct
     4261 cgactaaggt gagtgccacg atggtatcct ccccccgtag tttcattccc cgcactccgg
     4321 aggcattgcg cataaccgta cgaacggtct cttcatggaa acgtaggctg tagccgaaac
     4381 gggtggacaa cacgatctct ttgcttccat cggttgcaat gacatcgacc agctggtcat
     4441 tctctttgag ccggatggca ttggttccca ctgaacgcgg attggaaaat tctgtttggg
     4501 ggatcttgat gacctgcccg taccgggttg caaacagcat aaaccggtct tccctgaaga
     4561 cccggatcgg aatgaccttt gtaacgatct catcttttaa gttgaggagg ttgacgatag
     4621 gtttgccttt cgcaatgcgg gaactttcgg ggatctggta gactttgagc cagtagacac
     4681 gaccgatatt ggtaaagcag agcaggtagt ctttcatctc agcggcaaac acccggtcca
     4741 caccatcatc atctttggtg gtcattcccg tgattccgtg acctcccctc cgctgtttgc
     4801 ggtaggtatc gatgtccatc cgcttgatgt aattggcggt tgtaagcgag atcaggactg
     4861 tcttgtcctc gatcaggtcc tcattggaaa gatcgctggt atcaattgcg atctccgttc
     4921 tccgggcatc accgaatttt gccgcgatct ccacagtctc gcgccggatc tcgtccttga
     4981 tattggcttc gtttgaaagg atggtctcga gccttccaat ctcggcttca agtccctttt
     5041 tctcatcatt aatcttctgc tgctcgagcg ctgcgagacg gcgaagctgc atctggagaa
     5101 ttgcatttgc ctgcagttca tcgaggccga actgggtgat taatgccata cgggcgttat
     5161 caaccgaatc ggatgcccgt atggccgcga taattaagtc gattttggaa agggccatga
     5221 gcaggccgac taaaatatgg actttatcct gtgccttttt taagtcaaac tctgaccggc
     5281 gccgtattac ttcgatccgg tgttggacga agttctctaa aagtccgggg agggtgagca
     5341 ctctgggctg gttgtcgaca atcgcgaggt tgattgccca gaaactggat tcgagagcgg
     5401 tcagcttgta gagctggttt aagataactg ctgatatcgt tcccttccgg agctcaaaga
     5461 caacccggat accgtccttg tctgattcat cgcgtatgtc ggaaagcccg tcgatccttt
     5521 tatccttgac catttcggca attgcggtta tccactgtgc cttgttgacc tggtatggca
     5581 gctcggaaac gatgatgcgg tcgccacggt tgcccccttc actctcctca atggtggcaa
     5641 cacccctgat gatcacacga ccctgcccgg tggtatagat attcctgacc ccttcggtcc
     5701 ccatcattat gcctccagtc gggaagtcgg ggccgggcat aactttcatg agatcttcca
     5761 ccggaatttc cgggttgtcg agatagaggt tgaccgctgc gcagacctcc cgcaggttgt
     5821 gcggtggcat cttagtcgcc atgccgaccg caatgccatc cgtgccgttg accagcaggt
     5881 tgggaatctt tgacgggagg accgtgggct ctttcaacga ctcatcataa ttcgggacga
     5941 attcgacggt atccttatcg agatcctcta agagcgcttc tgcgtacggg aataatctga
     6001 cctcggtgta cctgctggct gcggcagaat caccgtcgac cgatccaaag tttccctgac
     6061 cctgtaccag catgttgcgg tacgagaacg gttgtgccat cttgacgatc gtatcgtaga
     6121 tggatgcatc cccgtgcggg tgaaacttac ccatcacatc accgacaacc ctcgcactct
     6181 tcttggtggg tttgtcgctg gtattgccca tctcccacat ggcaaagagc gagcggcggt
     6241 gaacgggttt gaggccatcg cggacatcgg ggattgcacg tccgatgatg acactcatgg
     6301 cgtaattaat atatgaggtc ttcatctcat gcgcgatact gataggttcg gtacggtgcg
     6361 tttcagggcc gcttgcgggg ggtacttcag atgtcaaggt tggtcacctc cttggcatgg
     6421 cggatgatga aatttttacg tatctccacg tcttttccca tcagcgtcct gaacatctca
     6481 tctgcctcaa gggcatcttc gatggtgacc tgcttgaata cccggtattc cgggtccatg
     6541 gtggtgtccc aaagctgctg ggcgttcatt tcaccaagac ccttgtaacg ctggacagaa
     6601 acgccttttt cgcccatggc agccgaggcg gtcttcatct cttcttccgt gtatacccat
     6661 ttctcttcct ttcctttcga gatacggaac agagggggct gggcgatata aatatatccg
     6721 gcttcgacca gcttgagcat gtaacggtaa aagaaggtca aaagaagggt gcggatatgg
     6781 gcaccatcca catcagcatc ggtcattatc acgatgtggt ggtagcgggc ccgttcctgg
     6841 tcgaatttct cgccaatgcc cgtgccgatt gccgaaatga gcgtctggat ttcagcgttc
     6901 tttaagatct ggtgttcacc ggctttttcc acattgagga tctttccccg gaggggaagg
     6961 attgcctgga atttgcggtc ccgaccctgc tttgctgaac cgcctgcaga gtcaccctcc
     7021 acaatataga cctcgctctt tgccggatcg cgttccgaac agtcggcgag tttgcccggg
     7081 agccctgaac tctcgagcga gctcttgcgc cgggcaaggt ccctcgcatt gcgtgcagct
     7141 tccctcgcct ttgctgcgga aagtgccttt tcgatgatga tcttgagtac attcgggttc
     7201 tcatcgaaat attcgctcat tgctgcatag accagcgagt cgaccgtgcc cttcacactg
     7261 ctgtttccca ggcgcatctt ggtctgtcct tcgaactggg ggtttgccat cttgatggag
     7321 acaaccgagg tgagaccttc cctcacatct tcgcccctca aggtgatggc tgagttttcc
     7381 ttgatcaggc catttctctt tgccaccgta ttgatcgtac gggtgattgc gctgcggaat
     7441 ccttcgaggt gtgtcccgcc atcccgggta ttgacggagt tgacatacga gtagattttc
     7501 tcgtcatatg cggtggtgta ctgcatcgcc acttcgattt cgagcttgtt ttctatgtcc
     7561 ttttttgaga tatcaatagg gaacgggtgg aggcattcca ccccttcgtt taagtatttg
     7621 acaaattcaa tgagcccgcc tgcataacaa taggtagctg tatcactgga ccgttcatcc
     7681 gtgatggtaa tcgttaggcc cgcattgaga aatgccagtt ccctcatacg gtgtgcgagc
     7741 acgtcataat cgaatgtagt ggtctcaaag atggtggcat cggggacaaa gtggaccctt
     7801 gtaccggtca tcttcgggcc gaactgtgca aggaaagctg cgcggctctc gtgctcagac
     7861 tcagctggac cgatcacctc cgatgtacgg gagaaggggg caggtgcccc gtaccactgc
     7921 tggtaacggg cgagcagctc gttgagcgac tcttccctcg tggaaagcgg ggatgtcatc
     7981 aaacccttgg aaa
//
LOCUS       DASRLV010000052         3411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_988782,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000052 DASRLV010000000
VERSION     DASRLV010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988782"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VFC43_02230"
     CDS             complement(<1..412)
                     /locus_tag="VFC43_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30037.1"
                     /translation="MEKIVRTITCGVVVLIAVLALFSGAGAAGQMQVSLGNVVKLSGY
                     SYSGDTVYLFLTGPNLPTNGVALDNINRLAENGWGTRVNVDADGHWVYQWNTGSAGGR
                     LDAGTYTVWVADGPADRSHLSTVEFSTISVSLSVP"
     gene            complement(555..896)
                     /locus_tag="VFC43_02235"
     CDS             complement(555..896)
                     /locus_tag="VFC43_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HZK30038.1"
                     /translation="MDYLYTLLKFIIGGSVIVGVALLAEHVDPRYGGMLAAAPIITTL
                     AFLFTYSEAGRETTHQLVISAFYFAIPSLMFLLVLYFLMARFCLLLSLGGAYGIWIGA
                     VLVVNRIVTGG"
     gene            1931..2674
                     /locus_tag="VFC43_02240"
     CDS             1931..2674
                     /locus_tag="VFC43_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30039.1"
                     /translation="MKLIVPFSLCIVVGLIVATGCVTTTTKHAENTTTTITITPTVTT
                     PGSGVNKTANTTSNSTSVSNLKGSLRVSISGILNPANLTVILDNETVGAVSPANPLYL
                     ITSEGNHTVMVCMDSVCEQESVMTKFGTYVTVDFSDRVKKNMKFPNPLARPDARILDY
                     FKNGNGISVHVEFINPSKEDLMMMASVSCGYSYIDDRTSFKMGDSAKGKLLQNVKAGQ
                     RVTATLDLYFVSGRSYSFDIPAIDELTIK"
     gene            complement(2970..>3411)
                     /locus_tag="VFC43_02245"
     CDS             complement(2970..>3411)
                     /locus_tag="VFC43_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZK30040.1"
                     /translation="LLYRNEKKYAEAVVAFNKALEINDKLPYVWNELGLCYREMKDNN
                     NALKSLTRAVELAPDNPELLFNLGEILELIGVLNMSNKYLDSAIATFKTVINQLPNNK
                     DACNHIGICYKEKGQIEESKFYFDRARDIRIGNKDTPIVPKRDF"
BASE COUNT          935 a          820 c          807 g          849 t
ORIGIN      
        1 cgggcaccga aaggctgaca gagatagtgc tgaattcgac cgttgagaga tgggaacggt
       61 cagccggacc atcggcgacc cagactgtat aggtgccggc atcgagcctg ccacctgcag
      121 aaccggtatt ccactggtag acccaatggc cgtctgcatc tacatttacc cgggttcccc
      181 agccattctc agcaaggcgg ttgatgttgt caagcgccac gccatttgtc gggaggtttg
      241 gaccggtcag gaagagatag accgtgtcac cgctatacga atatcccgac aacttcacga
      301 catttcccaa agagacctgc atctgccctg ctgcaccagc cccggaaaag agagccagca
      361 ccgcgataag cacaaccacc ccgcaggtta tggttcgaac aattttttcc attctatcct
      421 cacggtaatc ctgtaggtcc cgtatcggcc tgttagttgc aggttgaatg gggattatga
      481 acaggttacg aaagatctaa agtccgatga tataattctg ccgttgttgg ttggtatgca
      541 ccgggtgttc aaaatcaccc acccgtaaca atccggttca cgacaagcac tgccccgatc
      601 cagatcccgt atgcgccgcc gaggcttagg aggagacaga accgcgccat aagaaaatag
      661 agaaccagca ggaacatgag agaagggatt gcaaagtaaa aagccgagat gaccagctgg
      721 tgggttgtct ccctccccgc ctcggaatat gtgaagagga atgccagggt cgtgatgatt
      781 ggcgccgctg ccagcatgcc gccgtaccgt ggatctacat gctcagcaag cagggccact
      841 ccgacaatca cgcttccgcc gataatgaat ttcaggagcg tgtagaggta gtccatttta
      901 tggattgttt tgccgggctg tgttaaatat ccggttgttc cgagagagcc cagtgatata
      961 ccacattctt aacttacctt gatcggggga atactaaaat cgggaaaatt aagccctata
     1021 cgagctccga tttgccctgg gtttttagct gtccaggaga aaaacccgta aaacgctctg
     1081 tttcggttcg cgtggagaat aagcctaatt ttcgccattt ttgccaaaat acggctgttt
     1141 tccacctcac ctgacgaccc gtacagccct ttgcatcgag ctgttttttg cctcttactt
     1201 tataccgtct gaccctcaca aatcctgcca gaacaccaat gtgaggatat ttttttctcc
     1261 gacgatggtg taaaaaatgg gcagttttta aaggtagacg atgatagcag tggagcgggg
     1321 gtcgtacccc atcaggccgg gtccggcata tggcaggctc caggaaaggt atgatgcaga
     1381 aggtaggaca gtatctcaaa taagcccata atatgctcct tttaacaaac ggacgaagtt
     1441 gttttttgcc cagtgaaaag agtgtcaacg ccgagaaaaa atacggaata aaacaagtat
     1501 tttccttatt ggactgattt acagggattt taacctgtca atttagcggt cgtccagcag
     1561 cccccgtttt tcgctggata ggtagcacgg tcactatgaa aggaacacct gttttcgcgg
     1621 aatcgatcat cccggcccgg gacatcaggt gactttcgtt cgtcaaaact cacttctccc
     1681 tcggacacca cttcttcaca aaaagggcct ggtcctcatt tccgcacaaa acgttgaatt
     1741 ccgccagaca aattttggga aacttcggaa tgtgcacaga tagtggcagt ggcagaaatt
     1801 gccaaaaatc atcggacaaa aaagccatcc catccttaca atcactgcat ccgaaccaac
     1861 ctgcattgct ccagtgcctt gcaatatact aatatcgaaa aacaaacaaa cgtcgtgtaa
     1921 caaacatcct atgaaactga tagtaccatt ttccctgtgt atcgttgttg gactgatagt
     1981 ggctacgggt tgcgtcacga cgacgactaa gcatgctgaa aatactacga ccactatcac
     2041 tattactcca acggtcacta cgccgggtag tggggtaaat aaaactgcaa acactaccag
     2101 caacagcact tcagtttcga atctgaaagg atcactcaga gtatccatca gtggaatttt
     2161 aaatcctgct aacctgacag tcatccttga caacgagaca gttggcgctg tatcaccagc
     2221 caatccgttg tacctgatta cgtccgaagg caaccatacg gtcatggtct gcatggattc
     2281 ggtttgtgag caggaatcgg tcatgacgaa gttcggaacg tatgtcaccg tcgatttcag
     2341 tgaccgggtg aaaaaaaata tgaaattccc gaaccccctc gcacgacccg atgcacgaat
     2401 ccttgattat ttcaagaacg gtaatggaat ttccgtgcac gtggagttta tcaatccttc
     2461 aaaagaagac ctcatgatga tggcaagtgt gagttgtggt tatagctata tcgatgaccg
     2521 gaccagcttc aaaatgggag attcagccaa gggtaaatta ctacagaatg tcaaagcggg
     2581 gcagcgggta acagcgaccc ttgacctcta ttttgtcagt ggccgcagct atagttttga
     2641 tattccggct atagatgaac taacgataaa ataaaacgcg cttcttttta cagagtcctt
     2701 cattttcaca ccgtccctgg caatctgttc ttaccagagt aattttgaac ctgcaaaaca
     2761 tggaatcgtt ctggtgcatg aagaaaaatt cgattgtcaa ctggattaat aaatcagaag
     2821 atttcagtcc cgcgttaata ccctgtctca gggccggatg cgatcggtgg aaggaggggg
     2881 agtgcatcca tatccgcaaa gtgggaaaat gacgatgatg aagagaactg gcttgaaaaa
     2941 gcaatggagg agaaaagagg ttgcgggtat taaaaatccc gctttggaac gatgggagta
     3001 tccttgttcc caatacgaat atccctggca cgatcaaagt agaattttga ttcctcgatc
     3061 tggcccttct ccttatagca gatgccaata tgattacatg catccttatt gttgggcagc
     3121 tggtttatca ccgtcttgaa ggtggcaata gccgaatcca ggtatttatt gctcatgttg
     3181 agcacaccga tcaattctag gatctccccc agattgaaca ggagttccgg gttatccggt
     3241 gccagctcga cagcccgggt gagtgatttg agcgcattat tgttgtcctt catttcacgg
     3301 tagcagagac caagttcatt ccatacatag ggaagtttat cattgatctc cagggcttta
     3361 ttgaaagcaa ctaccgcctc ggcgtatttt ttttcattcc ggtaaagtaa a
//
LOCUS       DASRLV010000053         2916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_825602,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000053 DASRLV010000000
VERSION     DASRLV010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825602"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(358..522)
                     /locus_tag="VFC43_02250"
     CDS             complement(358..522)
                     /locus_tag="VFC43_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30041.1"
                     /translation="MISIVRGFAYLSGHLPFIPYSVTLERYPARTYFVFTAELKALLS
                     HEASISAPAF"
     gene            complement(537..776)
                     /locus_tag="VFC43_02255"
     CDS             complement(537..776)
                     /locus_tag="VFC43_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30042.1"
                     /translation="MIGEFFTNLLDRAKKAAKALKAKVTALVHKLLPPVTAKKSLVPS
                     IPLQIVQAMHISRRYRLTSDHSPIFSLYTFFPDRQ"
     gene            1141..1419
                     /locus_tag="VFC43_02260"
     CDS             1141..1419
                     /locus_tag="VFC43_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30043.1"
                     /translation="MVKTGEQPTEKILVTPATKQELTMLKQPGERYGDVVARLVREQK
                     QQDYIAQILRVAEEGDFVRLDSDEEYVQIRDEVKQSAPRDRSARARVH"
     gene            1403..1636
                     /locus_tag="VFC43_02265"
     CDS             1403..1636
                     /locus_tag="VFC43_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30044.1"
                     /translation="MLGYINSLPEKDRRIIKQHLVQLEDPVTARDAEHLRDGVCRMHI
                     THTYTAFFVVFRDCVRIVDVLTIEQVHKKYKRL"
     gene            complement(1781..2011)
                     /locus_tag="VFC43_02270"
     CDS             complement(1781..2011)
                     /locus_tag="VFC43_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30045.1"
                     /translation="MTQSTGRRNSTRRVVMTPGTWATLSRLKKPGQTSDTTIAGLITD
                     HQRLQLTVDPDAIDATEKMVSWKKAKKALGLK"
     gene            2412..2915
                     /locus_tag="VFC43_02275"
     CDS             2412..2915
                     /locus_tag="VFC43_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30046.1"
                     /translation="MADFVQNSQTKNAVRMLASPIADVTAFNAIVQSVITDNPFACVA
                     YMTAGTNHAPVEKTRESYTARINYEDGNASNVGNCSHRFNTLAGFSAGVTALLAAAAV
                     TTAHTGTAVHDPGDDNFSATLRCHDANGELYMLNFSRDRVTLTSYSDEAIRTSVETWA
                     DTVPALA"
BASE COUNT          713 a          720 c          766 g          717 t
ORIGIN      
        1 tcgtcttaga cacgatcacc cgttccagta tattgccatg ggtgacgact ccccgcttca
       61 caatatccgg gatgagatac agggggatgt cccgctcaat cttcgccggt cgccggggca
      121 tcaggacctc ccccgtgtac cggggtgaag gggtttttcc ggtatggttg tcctgtcgtt
      181 atgtgaaatt cctgcattga ttctcatggt ccgggcgaag aatcgggtac tcccgtttct
      241 tcccgtctgc tttattgatg tccaggagga cctcgttgga ggggtttttt gattggttgt
      301 catgggtaat cacgggtgaa attgtatcaa ttgttctttt gtccggggta atttttctta
      361 aaaagctgga gcggatatgc tcgcttcatg ggagagtagc gctttgagtt ctgcagtgaa
      421 cacaaagtaa gtcctggccg ggtatctttc cagggtcacg ctgtacggga taaacggcag
      481 gtgacctgaa aggtacgcga aaccacggac tatggaaatc accggaattc cggcgcttat
      541 tgacgatcag gaaaaaaggt atagagggag aaaatggggg aatggtcgct tgtcagccgg
      601 tagcgcctgc ttatgtgcat tgcctgcacg atttggagag ggatactggg cacgagggat
      661 ttttttgctg taaccggggg caggagcttg tgtaccaaag ctgtgacttt tgctttgagg
      721 gcctttgctg ccttctttgc ccggtcgagc aggtttgtga aaaattcccc gatcatcgtt
      781 tctcatcagt caggcactcg tgaggattta aattcagatg acccaatcag ctcgagtaat
      841 gttatgtctg acgaatgtac ggcatggatc cccctaaagg tgtgagcatg catacgtggt
      901 gcaggtatac ctggaattca ggtatccggg gagatttttc ccgggatgtt acaggtccca
      961 agagagggtg ggcagacctg acggtttcca tgaatggggt gaatgtccaa aggtttgtgg
     1021 cagtcgtatt gaggaggaaa agtgttgtca ccgaatcttt catataataa aaaggccaaa
     1081 aagtatattc gtcctttttt ggacaaacag ccaattatca taaggaatgt gcgataagat
     1141 atggtgaaaa cgggtgagca gcccaccgaa aaaattctcg taaccccggc cacaaagcag
     1201 gaactcacca tgctcaaaca acctggagag cggtatggag atgtcgttgc ccggctggtg
     1261 cgggagcaga aacaacagga ctacattgct cagatattga gggttgcaga ggagggcgat
     1321 tttgtccgct tggatagtga tgaggaatat gttcagatac gagacgaggt gaagcaatct
     1381 gcacctcgag atcgatcggc acgtgctcgg gtacattaac agcctgccgg aaaaagaccg
     1441 gaggatcatc aagcaacatc ttgttcagct cgaagatcca gtaaccgcac gggacgccga
     1501 acaccttcgc gacggggtat gtcgtatgca tatcacacac acgtatacag cattctttgt
     1561 tgtgttccgt gattgtgtcc ggatagtgga tgttctcacg attgaacagg tgcacaagaa
     1621 gtataaacgg ttgtgagaag atcaacacta tgccggtttt tactaaaggc ggctgccgta
     1681 aggaccagaa caccggtgtt attttaaaaa ggggtcccct gttttaaaaa taccggtctg
     1741 acgtagtgtc ccgggtcaat ggtagtgcaa aatcaggggg tcacttcaga ccaagggcct
     1801 tttttgcctt tttccacgac accatctttt ccgttgcatc gatagcatca ggatccacag
     1861 tgagctgcag ccgctggtga tcggtgatca gaccggcaat cgtagtatct gatgtctggc
     1921 cgggtttttt tagccgggag agcgttgccc atgtgcccgg tgtcattaca actcttctgg
     1981 tgctgtttct ccttcctgtg ctttgtgtca tccgttcacc ttcatagtat cattccggga
     2041 ctgatgtact ttgtaaaatc cccacaccag gaacattatt gccatcccgg cacactgcag
     2101 cggcggtcat gcacgtgaaa tagtggatct tcgtcattac accatcccgg ttcaatcacc
     2161 tgtgaaaagc tcatcagtat ttcgtgtact gctcatgccc atgcgttctg ccctttcatt
     2221 tcttacaagg aacataaatg ttccttccat ttcccgcatt aaaacgcccg gtttgctcgc
     2281 cactcattcc agcgtccccc atttcccgga aattccaggt tataataaaa gaccttactc
     2341 ccgtatgccc cataggagta tgtcaaaggg ccccgcgaca agagacaaaa aaacgaacaa
     2401 ggagaaaaaa aatggcagat ttcgtacaga actcacagac caaaaatgcg gtccgcatgc
     2461 ttgcgagccc gattgcagat gtaacggcgt tcaacgcgat tgtccagtcg gtcataaccg
     2521 acaacccgtt tgcctgtgta gcgtatatga ccgccgggac aaaccacgcc ccggtagaaa
     2581 agacccgtga atcatacacc gcacggatca actacgagga cgggaatgca agcaacgtcg
     2641 gcaactgttc ccaccggttc aacaccctcg ccgggttcag cgccggggta accgcgttgc
     2701 tggcagccgc tgcagtcaca accgcacata ccggtaccgc agtccacgat cccggcgatg
     2761 ataatttcag tgccaccctc aggtgccacg atgccaacgg cgaactctac atgctgaact
     2821 tcagccgtga ccgggtcacc ctcacctcct attcggatga ggcaatccgg acatcggtcg
     2881 agacctgggc ggacaccgtc ccggccctcg cataac
//
LOCUS       DASRLV010000054         5999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_666869,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000054 DASRLV010000000
VERSION     DASRLV010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666869"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VFC43_02280"
     CDS             <1..706
                     /locus_tag="VFC43_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30047.1"
                     /translation="ALTFLPVSGIVYPLPTTSSAGLNATIPDNSNVIATMAAKHFVSA
                     TVSSGSPAVGEPVTIGGRVTGGVLSEGVQIWVFAGNYVNVSHVPVNANGTFSSTFPTT
                     GLPLATYYVYIQSPGANGMFNIDLEDKGIYAGQVVNTGTNALIFNFTGTGGVQDAAAS
                     RALSDAINNQGVDDAYTKLTFQLVPPTTTPITVETSGVPDPAASATKSPLPLSITGLA
                     VVIAASSAVLYVRKRG"
     gene            1055..1285
                     /locus_tag="VFC43_02285"
     CDS             1055..1285
                     /locus_tag="VFC43_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30048.1"
                     /translation="MVKRLVDVNATYEIALGRKRAFGDETKGNSGLKISKKNENRLAA
                     VMLDGESLDDVLSRILDHYEKQFEKNECIFLP"
     gene            complement(1416..2504)
                     /locus_tag="VFC43_02290"
     CDS             complement(1416..2504)
                     /locus_tag="VFC43_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK30049.1"
                     /translation="MIRGAVAIFKRDFKKFLSNPFVVIMTLMMPIMYLVIFGNAFGGT
                     ISHVPVGVVQEAPPYNNTPLFEQATVALNHIPQKDSAKLLDVTVYADETSAKRDLSKG
                     LISAVVVFPSEISHDHAVRLYVDSSNSIIPSLVESAVRSVLAALGAHNPVAVNKIYGD
                     IKYIQFFGVGVIMMAIFTSTMFGGGIALIRDREAGIHEGYLVTPVKRTSIIAGIISSG
                     TVRAFIAGFTIFLIDIFLTGITIQSFEDFLLVILVILIACVGVTSLVVSFASRFSAQQ
                     EYASVVAFLNLILFMTSGAFYPVLGMPDWLRWITIINPEYYGVDALRGIILRGQGLDV
                     IGMDLIALLIFSGAMFILGIATYRRTLE"
     gene            complement(2501..3571)
                     /locus_tag="VFC43_02295"
     CDS             complement(2501..3571)
                     /locus_tag="VFC43_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZK30050.1"
                     /translation="MPVSNEKGKPSDLVDPIISCSSIPDDSSIIRIHHLSKIFKEKNR
                     IVTAVDDVTFDVRRGEIFGLLGPNGAGKSTLIRVLTTLLHPTSGTACVDTYDIVRDPE
                     KIRSIIGVCPQNSTLDVELTAYDNLEFYGKLVNVNDRLLDKRIWELLEMTELSDRAHM
                     KVQTFSGGMRRKLEIVRAFIHHPLILFLDEPTIGLDPEARHEVWQQIARLNKEKTTII
                     LTTHYMDEAEKLCDRIAFVDKGRLIALDILENLKQLIPEGDLIEIGIDRIDDSILQVL
                     RETALINSVEVKDKRVIISAKNGSKVLPEIVTVFEKFSVAMTSISIRSPSLEDVFIHL
                     TGKKLDDPGNQETKPSRGGRSP"
     gene            3718..4779
                     /locus_tag="VFC43_02300"
     CDS             3718..4779
                     /locus_tag="VFC43_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30051.1"
                     /translation="MITLRFYRIYDIGREIDLDWLEQALAKNYFTMRTSFVRVKQKSI
                     MIEDPPLMIQMHPIRVERQGKPFEFSVVARVYDIGAISFCFVHENPDSDYSTLEEIAF
                     LFAGQEGLSEFYVQYLKTLGEIIRPHIKNFAINPDFFEDYTVYVTDRRDDSIDPVPLL
                     LGEKINTSTQMREEIIKNSLSYSTDDLAILSWDSALLCNPEIPTDLIDLIEFANVQVL
                     ELRYYDRELTREMEKMYDDIEHADRLSAFRRSRQYHAIMAKQMETYAEISEVIEKVDN
                     LIKVTEDIYYARVYATALKVLRSNQWSESVTRKIDVIRENYSMLSDEVRIQHSNFLEW
                     VIIILIALEFGLAIWESIR"
     gene            4799..5281
                     /locus_tag="VFC43_02305"
     CDS             4799..5281
                     /locus_tag="VFC43_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HZK30052.1"
                     /translation="MVFECQQCGECCHYMGQVHTIKETLGEYEFLINNQYTGEKTMVI
                     VDKDKYQIFADKDIFLKQPEACPFLRYRAGEKKAYCTVHITRPEICRDYGCWRLLILD
                     ASGSRAGRIMFQRTFFSDDENLARLWKTSIDPINELDDAIWETKIREILTSAGYTVRD
                     "
     gene            complement(5296..5457)
                     /locus_tag="VFC43_02310"
     CDS             complement(5296..5457)
                     /locus_tag="VFC43_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30053.1"
                     /translation="MQHTEKNIRIIRDKLSRIGIGEKKMDDETILASYGINKFGQLCA
                     IPKVIPPVQ"
     gene            complement(5632..5937)
                     /gene="eif1A"
                     /locus_tag="VFC43_02315"
     CDS             complement(5632..5937)
                     /gene="eif1A"
                     /locus_tag="VFC43_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor eIF-1A"
                     /protein_id="HZK30054.1"
                     /translation="MDPNAVNEDGTPVVRVRLPKKWANEQFAFADLMMGANHIRVRCY
                     DGITRMGRIKGKIKKRVWIREGDILIVTPWSFQDEKCDIIYRYLPPQADWLRKNRYL"
BASE COUNT         1689 a         1341 c         1357 g         1612 t
ORIGIN      
        1 agcacttacg tttctcccag tctccggaat agtgtatcca cttccaacga catcttcggc
       61 aggattaaac gccacaatac cggataattc gaacgtgatt gccacaatgg ctgcaaaaca
      121 ttttgtaagt gcaacagtct cctcgggctc gccagcagta ggtgagccgg ttacaatcgg
      181 agggagagta accggaggcg tcctatcgga gggtgtacag atctgggtgt ttgccggcaa
      241 ctacgtgaat gtttcacatg taccggtcaa tgccaatggg acattctcca gtacttttcc
      301 aacaaccgga ttgccccttg caacgtatta tgtgtatatc cagagccccg gtgcaaatgg
      361 gatgttcaat atcgatttag aggataaagg catctatgcg ggtcaggttg tcaataccgg
      421 gaccaatgcg ttgatcttca attttaccgg aacaggaggt gttcaggatg cggcggcctc
      481 gcgggctctc tcagatgcaa tcaacaacca gggcgttgat gatgcgtata caaaactcac
      541 tttccagctg gtacccccca ctacaactcc gatcaccgtg gaaacctctg gtgttcccga
      601 tccggcagcc tcagcgacca aatccccact ccccctttcg atcaccggtc tggcagtggt
      661 catagccgca tcaagtgcgg tgttgtatgt aagaaaacga ggataacgtt tttagagacc
      721 ttaaggaaat tccttttttc agattttttg ataataattc atgtgccata cccgatctac
      781 gggctcgaac aatccccaaa taaaaagaaa acggaaacac gatgaaagtg ggttatttgg
      841 ggtaatatct gtcggaatca tcctggatcg ttatatgatt tgttataaga ataaaatctg
      901 gcttggtgtt gatgcaatac aatctgcgtc gttcaatttg atattttggg gttatcttca
      961 gaagttgggg ttcaagggga aatattcaat cagaaggaga aacaaagaga ttttgtacag
     1021 caagaagact gcgttataac tatgaggata aactatggtt aaacgtctgg ttgatgtaaa
     1081 tgcaacgtat gaaatcgcgt tggggagaaa aagagccttt ggcgatgaaa cgaaaggcaa
     1141 cagcggtttg aaaatttcga aaaagaatga aaaccggctg gcagcagtga tgcttgatgg
     1201 agagtcattg gatgatgtac tttcccggat attggatcac tacgaaaagc aatttgaaaa
     1261 gaatgaatgc atttttttac cctgaaaagc gtttatagta aactcccctt tcctgatctt
     1321 tataaataaa aacaaaaaac cccatccaaa tgtccaaaaa tacactaata gagtgtatcc
     1381 tttaaaaaaa gtatcaatta ttccttgtat ttttctcact caagcgtacg ccggtacgtt
     1441 gctataccca gaataaacat agccccggaa aatatcaaca atgctataag atccattcca
     1501 atcacatcga gcccctggcc cctgagaata ataccgcgga gtgcatccac cccataatac
     1561 tcagggttta taatagtaat ccagcgtaac cagtctggca ttccaagtac aggatagaac
     1621 gcaccggaag tcatgaacag gatgaggtta agaaatgcga ctacggaagc atattcctgc
     1681 tgagcagaga accgtgaggc aaaggatacg acaagactgg tgacaccaac acaggcgatc
     1741 aggatcacaa gaataaccaa caggaagtct tcaaaactct ggatcgtaat acccgtcaga
     1801 aaaatatcga tcaggaagat ggtaaatccc gcaataaatg cacggacagt accgctggaa
     1861 atgatcccgg cgataatgct cgtacgctta accggtgtca caagataccc ttcgtggatc
     1921 cctgcttcac gatcccgtat cagggcaatc cctccgccaa acattgtgga agtaaaaatt
     1981 gccatcataa taacgccgac tccgaaaaac tgaatatatt tgatatcacc gtaaatttta
     2041 ttgacggcta caggattgtg tgccccaagt gctgcaagaa cacttcttac tgcggattct
     2101 acgagggaag gaattatgga atttgaacta tcaacataca gacgcacagc atggtcatgg
     2161 gagatttctg acggaaaaac aacaactgct gatatcaatc ctttagaaag gtcgcgtttt
     2221 gccgaggttt catccgcata tacggtaaca tccagcagtt tggcactgtc tttctgcggg
     2281 atgtgattga gtgctaccgt tgcttgctca aaaagaggtg tattattata aggaggcgct
     2341 tcctggacaa caccgacggg tacatgactg attgtcccgc caaaggcatt accaaaaatg
     2401 accagataca tgatgggcat catgagcgtc ataatgacaa caaacgggtt gctgagaaat
     2461 tttttgaaat cccgcttgaa gatcgcgact gcgccccgga tcatggtgag cgccccccgc
     2521 gtgaaggttt tgtctcctgg tttccgggat catccagttt tttaccggtg agatggatga
     2581 aaacatcctc cagtgagggt gatcgtatcg atatggaggt catcgcgacg gaaaattttt
     2641 caaagaccgt aacaatttca ggcaatactt tacttccgtt ctttgcagag atgatgaccc
     2701 tcttgtcctt gacttcaact gaattgatca gggcagtttc acgtaatacc tgaagaatac
     2761 tgtcatcaat cctgtcaatc ccgatctcga tcaggtcacc ttccgggatt aactgcttga
     2821 ggttttccag aatatccagc gcaataagcc gccctttatc cacaaatgcg atgcggtcac
     2881 agagtttttc tgcttcatcc atgtaatgcg tggtcaggat gatggttgtt ttttctttgt
     2941 tcaacctggc gatctgttgc cagacttcgt gacgcgcttc cgggtcaagt ccgatagtgg
     3001 gctcatcgag aaacaggata agcgggtgat gaataaaagc ccggacaatc tcaagtttcc
     3061 gtctcatgcc cccggaaaat gtctggactt tcatatgtgc acggtctgat agttccgtca
     3121 tctcgagcag ttcccatatt cttttatcca ggaggcgatc gtttacgttt acgagttttc
     3181 cataaaattc aaggttgtcg tatgcagtaa gttcgacgtc aagtgtacta ttctgcgggc
     3241 agaccccgat aatgctccgg atcttttccg gatcacgaac aatatcatac gtatcaacgc
     3301 atgccgtgcc tgaagtcggg tggagcagcg ttgtcagaac ccggataaga gtactttttc
     3361 ccgctccgtt cggtcccaaa agaccaaaga tctctcccct cctgacgtca aacgtgacat
     3421 cgtcaacagc tgtaacgatg cggttcttct ccttaaaaat ttttgaaagg tgatggattc
     3481 tgattatgct ggaatcgtca ggaatcgagc tgcagcttat tatgggatca acgaggtctg
     3541 agggcttccc cttttcattt gatacaggca tactcattat ttatctgatg gttcggtgtg
     3601 gtagtactta attcctgctt cttttaagca ataggataaa accgtagaaa ataattatcc
     3661 gtcatcaaca acaattctgc tccacaaaag gattcttcga attggaggaa gggcaccatg
     3721 attactctcc ggttttaccg gatttatgat attggccggg agatagatct tgactggctg
     3781 gagcaggccc tcgccaagaa ttactttacg atgagaacca gttttgtcag ggtcaagcaa
     3841 aaatcaatca tgattgagga tccccctctc atgatccaga tgcacccgat tcgggttgag
     3901 aggcaaggga aaccctttga attttcggtt gttgcccgtg tctatgatat tggtgcaatc
     3961 agcttttgct ttgtccatga aaacccggat tcagattatt caacacttga ggagatcgcg
     4021 ttcctgtttg ccggtcagga aggattatcc gaattctatg tccagtacct caagacactc
     4081 ggcgagatca tcaggccaca tatcaaaaat tttgcaatca atcctgattt ctttgaggac
     4141 tatacagtct acgtgaccga ccggagagac gattcgattg atccggtccc tctcctgctg
     4201 ggggaaaaga tcaatacttc gacccagatg cgggaagaga ttattaaaaa ttcgctcagc
     4261 tattcaaccg atgacctcgc gatactgtcc tgggactcag cactcctgtg taatcccgaa
     4321 atacctacag atctgattga cctcatagaa tttgccaatg tccaggtgct tgaactccgt
     4381 tactatgacc gggaattgac ccgtgaaatg gaaaagatgt atgatgatat cgaacatgca
     4441 gacaggttat cagcattccg gcggagtcgc cagtaccatg cgatcatggc aaagcagatg
     4501 gaaacctatg ccgaaatatc tgaagtgatc gaaaaggtgg acaacctgat taaagtcaca
     4561 gaggatatct attatgcccg tgtgtatgcc actgccttaa aagttctcag aagtaaccag
     4621 tggagtgaga gcgtgacccg gaagatcgat gtaatccggg aaaactattc catgctttct
     4681 gatgaagtcc gtattcagca ctcaaacttt ctcgaatggg ttatcattat cttaattgcc
     4741 cttgaattcg ggctcgcaat ctgggagagt atccgttgat tcagggagat taacgtttat
     4801 ggtttttgaa tgccagcagt gtggtgagtg ctgccactat atggggcagg tccacaccat
     4861 aaaagaaacc cttggcgagt atgagtttct gatcaataat caatacaccg gggagaaaac
     4921 catggttatt gttgataagg acaagtatca gatttttgcc gataaagata tttttttaaa
     4981 acagcctgag gcatgcccct tcctgcggta tcgtgcagga gaaaagaaag catattgcac
     5041 ggtccatatc acacgcccgg agatctgcag ggattacggg tgctggcgtc tcctgatcct
     5101 ggatgcttcg gggtcaagag ccggccggat tatgttccag cgaaccttct tttcagacga
     5161 tgaaaacctg gcccggctct ggaaaactag tatcgatccc ataaacgagc tggatgatgc
     5221 aatttgggaa actaagatac gtgagattct tacgagtgct ggatacaccg tgcgggatta
     5281 aaagaagata acgctctatt gaacgggcgg gatcacttta ggaatggcgc atagctggcc
     5341 gaatttattg ataccatacg atgccaggat tgtctcgtca tccatttttt tctcgccgat
     5401 acctatcctg ctcaacttgt cacgaattat tctgatattc ttttcagtgt gctgcataac
     5461 ggtacagcat atttctttta tgacacgata aagagatgtt ttttaaaaat acggcaaacc
     5521 ctttattttt aaaaaaaatc agtttaaata ttatctatgc cctttgagta aaaaccccag
     5581 gatcctctta taaatgcaca ttacctggag aaattttaaa agaagggatt ttcagagata
     5641 ccggttcttt cgtaaccagt ctgcctgcgg gggaaggtat cgatagatga tatcacactt
     5701 ctcgtcctga aaagaccatg gtgttacaat gaggatatcc ccttcgcgaa tccagactcg
     5761 ctttttgatt tttcccttaa tccggcccat acgggtgatg ccgtcatagc accgcacgcg
     5821 tatatgattt gctcccatca tcaggtccgc aaaagcaaat tgttcgttgg cccatttctt
     5881 tggtaaccga accctgacaa caggagtccc gtcttcattt actgcattcg ggtcgatacc
     5941 gtcattattt ccactgttat tatttctaat tcacacaact ccggtgaggt atatgaatg
//
LOCUS       DASRLV010000055         7326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1151196,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000055 DASRLV010000000
VERSION     DASRLV010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 7326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7326
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151196"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="VFC43_02320"
     CDS             complement(<1..1376)
                     /locus_tag="VFC43_02320"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase II small subunit"
                     /protein_id="HZK30055.1"
                     /translation="MLDAQQITLRFLETKLQVHPDVVRYIQEQNELELIDRIIAQVPG
                     ETIVVSAKHIPGMSATRDGTRFLSDPTVEIISGSPGTSGSVNGTSDYLHYFRDRYNRL
                     GNMIRSRCGAMPIEALTRNTRYRQEECTIIGMVVDVKTTTNGHRIAELEDSSANIPVL
                     FRKDRPVFSDAERIIHDEVIGVKGKLSNDGKLFFAEQIYRPDIRIDNTPYKSEQPGKA
                     VFISDVHVGSNTFLEDCWNRFADWLSDSDYSYLLIAGDLVDGIGIYPGQENELVIKNI
                     YEQYDAFGAMMQDLPSRMKIIISPGNHDVVRGAEPQPVIPQQFTKKFPGNCMVVENPA
                     LVNLQGVRVLMYHGRSIDDMIGLIPGASYEQSGIMMEEMLQRRHLAPAYGRRTPIAAG
                     KTDRLIIDPLPEILHTGHVHIRGLTQYRGVLGINAGTWQSQTAFQKQMNVNPTPAQAV
                     VVDLQTLIP"
     gene            1515..2135
                     /locus_tag="VFC43_02325"
     CDS             1515..2135
                     /locus_tag="VFC43_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HZK30056.1"
                     /translation="MYDIFQMLILGFVIGLTGALAPGPTLVATINASIAGNWATGLKI
                     SLGHVIVELFLVILILSGLAAVALPHATVIAAVGGIALILFGGLTIAGSRTATMSTSA
                     VQPVSNPYAAGLITSAANPYFWIWWLSIGSAMVIAGLEGGLLFVGIFMVGHWSADTVW
                     YTLVSIAVSKGRALFSDSAYRMIMVLCGIFLIIFGMFYLSPFIVSP"
     gene            2293..2928
                     /locus_tag="VFC43_02330"
     CDS             2293..2928
                     /locus_tag="VFC43_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HZK30057.1"
                     /translation="MSLARDLLWIGAVVGGIALALFLICGTWPAVVTIESQSMVPHMN
                     VGDLVVVVQKDRYGAFETWSDGKKSGRLMYAEYGDVIIYKPNGLTSIHPIIHRAIQYV
                     DNEPVTEIKGNKLLGNYTPPHPGYITWGDNNPRPDQGLNYPGIGIIEPVKDEWVIGKA
                     LFAVPLVGYLPLHIIEVTVVIIGIMILHEMYLRSVDQKKGKRPDKSGKRKR"
     gene            2943..4028
                     /gene="cofH"
                     /locus_tag="VFC43_02335"
     CDS             2943..4028
                     /gene="cofH"
                     /locus_tag="VFC43_02335"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HZK30058.1"
                     /translation="MSEISHLLSDVLGGHRLTVAEAEDLLKCNDRTIHRIAAAADEMR
                     ENRAGNIVTYVKNQNLHVTNICKNLCGFCGFGRSAEDEGAYCNDKATIQAKTRLAMER
                     GVTEICLLSGVHPDFSIDTYCDLISWVHEIGPYIHLHAFSPDEVSHAASGSTLSSVEV
                     LERLKAAGLGSLQGTAAEILVDSVRKVICPRKLCTAEWVRIIKEAHTLGIPSTATIMY
                     GSCETAHDRAEHLGILRDIQDETNGFTELVPLTYIHTNTPLYKAGIARAGATGREDLL
                     MIAVSRLFLDNFYNIQVPWGKLGLKMSQLTLLAGGNDLAGTMFSDDVSVNAGATDADY
                     LDPKVMERMVSDIGRTLKERTTLYKLI"
     gene            complement(4078..4377)
                     /locus_tag="VFC43_02340"
     CDS             complement(4078..4377)
                     /locus_tag="VFC43_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YunC family protein"
                     /protein_id="HZK30059.1"
                     /translation="MEQVRIQLTRKVADGYIIPLGPANLVAVMTDVGMVGCGAFDVAA
                     LDSFSYPAAKVRPVIGPSIVDTDDILKGIIKETNRSALGRGIRTGMTGREALDLL"
     gene            4510..5589
                     /gene="cofH"
                     /locus_tag="VFC43_02345"
     CDS             4510..5589
                     /gene="cofH"
                     /locus_tag="VFC43_02345"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HZK30060.1"
                     /translation="MENNLRTLLRDIKTGHRVSDDEALLLFNIRNRDVWKIATAADEI
                     REQRVGNAVTYVRNQNINVTNLCVNACGFCGFSKKQGDDGIYFHDKSEIQKKAGLAKM
                     REVSEICTVSGLHADFNTKSYCDVYRWIHEAAPGIHLHASNPMEVAYAAKKSNMSTQE
                     VLSAMKEAGLGSMCGTAAEILVDDVRKKICPQKISTDEWVRIIREAHEMGIPTTATIM
                     YGHCENDEDRVRHLSILREIQDETHGFTEFVPLSFIHMNTPLYHAGIARAGATGREDL
                     LMVAIARLFLDNFQNIQVSWVKEGIKMAQLSLIAGANDLGGTMFEESISKGAGAVNTD
                     YLDPAEMQRVAEDVGRTLRRRTTLY"
     gene            5641..6096
                     /locus_tag="VFC43_02350"
     CDS             5641..6096
                     /locus_tag="VFC43_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZK30061.1"
                     /translation="MKRTDREITDIFEIESVLNHATVCRIGLADAGEPYIIPVCFGYV
                     PGIIYLHSSLSGKKITMIEKNPRICFEVDQCDTIIQNKVPCAWGMHYRSVIGFGKASF
                     IADHNEKKQGLNCIMSHYGGGLHEFFDEEVRSVCVIRIDIDSMSGKKHD"
     gene            complement(6115..6669)
                     /locus_tag="VFC43_02355"
     CDS             complement(6115..6669)
                     /locus_tag="VFC43_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HZK30062.1"
                     /translation="MKVLGISGSMRKDGNTADLVNVILDRCHVEGIKTEFISLSGKKI
                     HPCLGCEKCKEKKWCVIENDDWDDLIQKVLDCDILIIGSPTYYYDVCGHLKNFIDRTY
                     SLYHDRKLAGRKGIAVAVYANKGGSRTIQTLEGFLSTHEFSSLGSVKGKGYQKGDVLS
                     DGDAVEKAQKIGDKIVRLVKRNHD"
     gene            complement(6666..7121)
                     /locus_tag="VFC43_02360"
     CDS             complement(6666..7121)
                     /locus_tag="VFC43_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HZK30063.1"
                     /translation="MDKGIYCLVFKTPGCTIRIGALGALLFQAGWYIYIGSAQGSGGL
                     QRLRRHISLSHLRDKQPTWHVDYLLTSPEFSLVYAVCAVSVDRYECLLSRELNGRRIP
                     RFGCSDCSCTSHLFYREEDPRDEILTAFRSLLLSPVIKTIINAQVQHNI"
     gene            complement(7114..>7326)
                     /locus_tag="VFC43_02365"
     CDS             complement(7114..>7326)
                     /locus_tag="VFC43_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZK30064.1"
                     /translation="VLISGDTVFSDGCFGRYDFPGGSRIELAQSLNRLSLLDVQGLYP
                     GHGEPVAHGGSRHIAAALGLMKSGYG"
BASE COUNT         1927 a         1697 c         1831 g         1871 t
ORIGIN      
        1 ggtatcaggg tctgaagatc aacgacaaca gcctgggccg gggttgggtt cacgttcatc
       61 tgtttctgga atgcggtctg ggactgccat gtaccggcat taatcccgag cacaccccga
      121 tactgtgtaa gtccccggat atggacatga ccggtgtgaa ggatctcagg gagtggatca
      181 atgatcaacc tgtcagtttt acctgcagca atgggggtcc tgcgcccgta tgccggagca
      241 agatgccgcc gctggagcat ctcttccatc attatcccgc tctgctcata ggacgctcct
      301 gggatcagac cgatcatatc atcgattgat ctcccgtggt acatgagaac cctgactccc
      361 tgaaggttga caagtgccgg attttcaaca accatgcagt tgcccgggaa ttttttggta
      421 aactgctggg gtattaccgg ttgtggctct gcccccctga caacatcgtg attaccgggt
      481 gaaatgatga tcttcatccg tgaaggaagg tcctgcatca ttgcgccgaa cgcgtcgtat
      541 tgttcgtaga tattttttat aacgagttcg ttctcctggc ccggatagat gccgatacca
      601 tcgacaaggt ctccggcaat caggagatat gagtaatccg aatccgaaag ccagtcagca
      661 aacctgttcc agcaatcttc gagaaatgta ttgctgccaa catggacatc ggatataaaa
      721 accgctttgc ctggctgttc gcttttgtag ggggtattat cgatgcgaat atcagggcgg
      781 tagatctgtt cggcaaaaaa aagtttcccg tcgttggaga gtttgccttt aaccccgatt
      841 acttcgtcgt ggataattct ttctgcatcg gagaagacag ggcggtcttt acgaaaaaga
      901 acaggaatat ttgcagaaga atcctctaac tcggcaatac ggtgaccgtt tgtggtggtc
      961 tttacatcga caaccatacc gataatagta cattcttcct gacggtaacg ggtattccgt
     1021 gtgagtgctt ctatcggcat cgccccacat cggctcctga tcatatttcc cagccggtta
     1081 taacggtcac ggaaatagtg aagatagtca cttgttccat tcacactacc ggatgttccc
     1141 gggcttccgc tgattatttc aacggtagga tccgacagga acctcgtccc gtcacgggtg
     1201 gcgctcatac cgggaatatg tttagccgaa actacgatgg tctcaccggg aacctgcgcg
     1261 ataatgcgat cgatgagttc cagttcattc tgttcctgga tataacggac aacatcggga
     1321 tgcacctgca gcttggtttc aagaaacctg agagttatct gctgtgcatc aagcatgaat
     1381 agtatatgca cgtggagaaa atatagggtt ttattatagc acggtaaaat ttacaaaata
     1441 cccgatcggg acagtgatga tgaaggattg ataatctttt cagacgaatt ttgtatgaag
     1501 gaactttcct caacatgtac gatatattcc agatgcttat tctcggattt gtaatcggat
     1561 tgaccggggc gctcgctccc ggtccaacac tcgttgcaac cattaatgca tccattgccg
     1621 gcaactgggc aaccgggttg aaaatctctc ttgggcatgt aattgtagaa ctttttcttg
     1681 ttatcctgat tctatcagga ctggcagcag ttgccctgcc ccatgcaaca gtcatagcag
     1741 cagttggagg gattgcgttg attttgttcg gcgggcttac cattgctggc agcagaaccg
     1801 ctactatgag taccagtgcg gtccaaccgg tcagcaatcc ctatgcggca ggactgatta
     1861 ccagcgcggc aaatccgtat ttctggatct ggtggctctc aataggaagc gcgatggtca
     1921 ttgccgggct ggagggggga ctgctttttg tgggaatttt catggttggg cactggagcg
     1981 cagacaccgt ctggtacacc ttggtttcaa ttgcggtatc aaaaggaagg gccctctttt
     2041 ctgattctgc ataccggatg atcatggtgc tttgcggaat cttcctcata atttttggga
     2101 tgttctacct ctcacctttc atcgtttcac cgtaaaatcc tgcggtattt tataatgcca
     2161 catggccaat tatgaaaggt taacaaaaaa actaaaaaat cgaatcaaga gcgttataaa
     2221 atggaagata atgataacaa tcgggatatc cgcgcgttga taataaaatt ccgaaaaagt
     2281 gatcactggg ctgtgtcctt ggcgcgcgat ctgctctgga ttggtgcagt tgtcgggggg
     2341 atcgctcttg ccttattcct gatctgtggt acctggccag ctgttgtcac catagaatca
     2401 cagagtatgg tgcctcatat gaacgtcggg gaccttgttg ttgttgtcca gaaggaccgt
     2461 tacggcgctt ttgaaacatg gtctgacggg aaaaaaagtg gcagactgat gtatgcagag
     2521 tatggcgacg ttataatcta caaaccgaac ggcctgacca gcatacatcc cattatccat
     2581 agagctatcc agtatgtgga taatgagccg gttacggaaa tcaagggaaa taaactcctt
     2641 ggaaattata ctcccccaca tccgggatac attacctggg gggataacaa tccacgtcct
     2701 gaccagggtc tcaattaccc gggtatcggc attatcgagc ccgtaaagga tgaatgggtg
     2761 attggtaagg ctctttttgc ggtgcccctc gtaggatatc ttcctctcca tatcattgaa
     2821 gtgaccgtgg ttatcattgg tatcatgata cttcatgaga tgtatctgcg ttcagtggat
     2881 cagaaaaaag gcaaacggcc agataaaagc gggaaaagaa agcgttagat ctgcaggtaa
     2941 ccatgtcaga gatttcacac cttctttcag atgtactcgg cggacatcgc cttactgtgg
     3001 cagaagctga ggatctcctg aaatgtaacg accgcactat ccaccgaata gcagctgctg
     3061 cagatgagat gagggagaat cgtgcaggaa atatcgttac ctacgtgaaa aaccagaacc
     3121 tgcatgtgac caatatctgt aagaacctct gtggtttctg tgggtttggc aggagtgcgg
     3181 aagatgaggg ggcatactgc aatgataagg cgaccattca ggcaaagaca cgtcttgcga
     3241 tggaacgggg tgttactgag atttgtctgc tgagcggtgt gcatcctgat ttttcgattg
     3301 atacgtattg tgatctaatc tcgtgggtgc acgaaatagg accgtatatt catctgcatg
     3361 cgttcagccc ggatgaggtc tcccatgcag catccgggag tactctttcc agcgtcgaag
     3421 tacttgaacg attgaaggcg gccggcctgg gatcgctaca gggtaccgca gctgagatcc
     3481 ttgttgattc tgtgagaaaa gtgatctgtc ccagaaaatt gtgcacagca gaatgggtaa
     3541 gaattatcaa agaagcacat accctgggga tcccatcgac cgcaactatc atgtacggct
     3601 cctgtgagac agcacacgac cgggctgagc atcttggcat cctgcgagat attcaggacg
     3661 agacgaacgg ttttaccgaa ctggttcccc tgacctacat ccacacaaac acgccgctct
     3721 ataaagcggg tatcgcccgt gcaggtgcaa caggcaggga ggacctgctc atgatagcgg
     3781 tttcccggtt gtttctggat aatttttata atattcaggt cccctggggg aagctgggac
     3841 tgaaaatgtc tcagttgaca ttgctcgcgg gtggcaatga tcttgctggt accatgttct
     3901 ccgatgatgt atcagtaaat gcaggggcaa ccgacgcaga ctatcttgat cctaaggtca
     3961 tggaacggat ggtttcagac attgggagaa ccttaaaaga gcgaacaacg ctgtataaac
     4021 tgatttaaga acagaagaaa aaatcaacaa aaaaaagttc ctttttactt atcgatatca
     4081 gagaagatca agcgcctccc tgccggtcat gccagtacgg attccgcgac ccagggcaga
     4141 tcggtttgtt tccttgatga tcccttttaa aatatcatcg gtgtcgacaa ttgaaggccc
     4201 gattaccggt cttaccttcg ctgcgggata gctgaacgaa tcgagtgcag caacatcaaa
     4261 agccccacac ccaaccatac caacatcggt cattaccgca acaaggttcg ccgggccgag
     4321 agggataatg tacccgtctg caacctttct ggtaagttgt attctaacct gttccatatc
     4381 tctccagtag agatgattgg tgcaggggga ttaataatca tggggcgtaa gaatacgata
     4441 cagatatttt tcttcaggat atcatgaata ccaaaaaccc ggatatctga aggatacact
     4501 ggaggagcca tggagaataa cctgcggact ttgttgaggg atatcaaaac cggccaccgg
     4561 gtaagcgatg atgaagcact gcttctgttc aacatcagaa atcgggatgt ctggaagatt
     4621 gcgactgctg cagatgagat tcgggaacaa cgggtcggca atgcagttac ctatgtcagg
     4681 aaccagaata tcaatgtgac caatctctgt gtcaatgcct gcggtttctg cggtttttca
     4741 aaaaaacagg gtgatgacgg tatctatttt catgataagt ccgagatcca gaagaaagca
     4801 ggccttgcaa aaatgcgtga agtatccgag atctgtacag tcagtggcct tcatgcggat
     4861 ttcaatacca aatcctattg tgacgtctac cgctggattc atgaggctgc tcctggcatt
     4921 caccttcatg caagcaatcc gatggaagtt gcgtatgctg ccaaaaaaag taatatgagc
     4981 acgcaggaag tgttgtctgc aatgaaggag gcgggactcg gatcgatgtg cgggacagca
     5041 gcagagatcc ttgttgatga tgtccgtaaa aaaatctgtc cacagaagat ttcaacggat
     5101 gaatgggtcc gcattatccg cgaggcgcat gagatgggca ttccaacgac tgcgacgatc
     5161 atgtatggtc actgcgagaa tgatgaagac cgggtgcgtc acctgtcaat ccttcgtgag
     5221 atccaggatg aaactcatgg ttttacagaa tttgttcctc tctcgttcat tcatatgaac
     5281 acccctctct atcacgcagg gatcgcgcgg gcaggagcga ccggaagaga agatctgctg
     5341 atggttgcca tcgcacgcct ttttctggac aacttccaga atatccaggt ttcatgggta
     5401 aaggaaggaa tcaagatggc ccagttaagt ttgatcgccg gtgcgaatga ccttggaggc
     5461 acgatgtttg aggagagcat ttccaaagga gcaggtgcgg tcaataccga ttacctcgac
     5521 ccagccgaaa tgcaacgtgt tgcggaggat gttggtcgaa ccctcagacg ccggacaacg
     5581 ctctactaac ttgcctgagc ggccttcgtc aatctattgg ataaattcac aagagggaat
     5641 atgaaaagaa ccgatcgtga aattaccgac attttcgaaa ttgaatcagt cctcaaccat
     5701 gcaactgtct gcaggattgg actggctgat gctggtgagc catatattat cccggtctgt
     5761 tttggctatg tacccggaat aatctacctt cattcttcct tgtccggaaa aaaaatcact
     5821 atgattgaaa aaaatccgag gatctgtttc gaagttgacc agtgtgatac gatcattcag
     5881 aataaggttc cgtgtgcatg gggaatgcac tacagaagcg ttatcggttt tggaaaagcg
     5941 tctttcatag cagaccataa tgagaagaaa caaggcctga actgcattat gagccattat
     6001 ggaggcgggt tgcatgagtt tttcgatgag gaggtcagat ccgtctgtgt aatccggatt
     6061 gatattgact caatgagcgg aaaaaaacac gattgaggaa aggtattttt aaaatcaatc
     6121 gtggttgcgt ttcacaagcc ggacgatttt atcgccaatt ttctgggctt tctctacagc
     6181 gtccccatcc gagagtacat cgcctttctg gtaacccttt cccttgacag aacccagcga
     6241 ggaaaattca tgagtgctta aaaacccttc aagcgtctgg attgtccggc taccgccctt
     6301 gtttgcatac acagccactg cgatcccctt ccttcccgca agcttccggt catgatacag
     6361 ggaataggtg cggtcgatga agtttttcaa gtggccgcag acatcgtaat aataggtggg
     6421 tgaaccgatg atcaaaatat cacagtcaag cactttctgt atcagatcat cccagtcatc
     6481 attctctatt acgcaccact tcttttcctt gcatttttca cagcccagac acgggtggat
     6541 cttctttccc gaaagtgaaa tgaattcagt tttgatccct tcaacatggc agcggtcgag
     6601 gataacattt accagatctg cagtattgcc atctttacgc atgcttcccg atatcccgag
     6661 cactttcata tgttatgctg tacctgtgca tttatgatag ttttgatgac agggctaagc
     6721 aggagtgaac ggaacgcagt gagaatctca tcccgggggt cctcctcccg gtaaaaaaga
     6781 tgcgatgtgc aggaacagtc actacagccg aaccttggaa ttctgcgccc gtttaattca
     6841 cgggagagaa ggcattcata acggtccaca gaaactgcac agactgcata tacaagcgaa
     6901 aattctggtg atgtgagaag gtaatcgaca tgccacgttg gttgtttgtc gcgtaaatgg
     6961 gaaagtgaga tatgacgtct gagccgttgc aaccctccgc ttccctgggc cgatccgata
     7021 tagatatacc agccagcttg aaacagaaga gctcccaatg cacctatccg gatcgtgcag
     7081 ccaggcgttt taaaaacaag gcagtagatt cctttatcca taaccgctct tcatcaaccc
     7141 aagtgctgcc gcgatgtggc gactcccccc gtgcgcaacg ggttccccat gacccgggta
     7201 aagcccctga acgtcaagaa gcgaaagccg gttgagtgac tgcgcaagtt cgatcctgct
     7261 tccccccggg aaatcatacc gtccgaaaca accgtccgaa aaaacagtat ctccgctgat
     7321 gagcac
//
LOCUS       DASRLV010000056         3828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_989880,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000056 DASRLV010000000
VERSION     DASRLV010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989880"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..421
                     /locus_tag="VFC43_02370"
     CDS             56..421
                     /locus_tag="VFC43_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZK30065.1"
                     /translation="MVHKILILEDDQPILDLMDMMIRRLGYEPVLITNGLEALDLIKQ
                     EPPDLILLDIMMVPINGWEFLERLRGELGKRELPVILFTASPMVSERMAEMKDPCLSV
                     LTKPVSFAELKAGIARYLK"
     gene            complement(1157..2992)
                     /locus_tag="VFC43_02375"
     CDS             complement(1157..2992)
                     /locus_tag="VFC43_02375"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HZK30066.1"
                     /translation="MRLLLIHSDYIEYEAKKKTKMAEDCSVLSDRETEVLTVFCAVES
                     IDEEDLQGVVLQAIDEVKKTAGQVHVDKIVVYPYAHLSSDLASPETAVATLNALKTGL
                     EQEGFFVKRAPFGWYKSFKISCKGHPLSELSKTIVPGEGGTAKSEKSEITHDWFVMTP
                     DGKSHDYKEFLDDSPFGCLVKKELGVAVPTGGEPAHVGLMRSKELVDYEPASDVGCLR
                     WMPKGKIIRDLLSDYVLRLVLEYGGTPVETPVMYDLGDRAIYEHAEKFGERQYRFKSN
                     NRNMMLRFAACFGMFSIMRDMHISPHNLPMKMYELSTYSFRHEQKGEVIGLKRLRAFT
                     MPDMHSLCTDMPEALKCFEEQLAMGWQTGKDFETKLVAAFRCTKDFYAQNEAWVKKIV
                     KESDCPMLIEILSDRVHYWVAKIDLAAIDGQMRPIENPTVQIDVESSTRFNIKYHTDD
                     GTVVHPPILHCSPTGSIERVICAILENLSTQAVPSLPTWLSPVQVRVVPVAERHGAFA
                     EEVCAQINTAQIRCDVDDREESVGKKVREAGMDWIPFVVVVGDEEAASRKLTVTIRRK
                     SQPNKPAKEQLTMDELIEAVHKDTEGKPFRPLYTPRKLSKKARYI"
     gene            complement(3126..3719)
                     /locus_tag="VFC43_02380"
     CDS             complement(3126..3719)
                     /locus_tag="VFC43_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30067.1"
                     /translation="MKKAWIIVIILLVFTTVSTVSASGPIHFSYTGDLVVTYISGTAG
                     YNNEFGIETSQPVSLGFTQENLTPVPGATYKSLGHCTSDEPVVLYCKSPLEGGSVTYY
                     SNQTSGDGFDHAVVTMLEDGSYRVGFEDMFGARENPAVPGDGDYDDVVLNVACIRYAA
                     PPDEGGPVRNFPVMALPAGFICGIIGSALYIHRTREP"
BASE COUNT          961 a          934 c          945 g          988 t
ORIGIN      
        1 gaaaccacgt tctgcataca catcccgaaa aacaaacctg cggagacgga aaataatggt
       61 acataaaatt ctgatactgg aggatgatca gcctatcctt gatctgatgg acatgatgat
      121 ccgaagactc ggctatgagc cagtactgat aacaaacggt cttgaggcac tcgacctgat
      181 aaaacaggaa ccacccgacc tcatcctgct ggatatcatg atggtgccca tcaacgggtg
      241 ggaattttta gagagactac gcggagaatt agggaaacgg gagctgccgg tcatcctttt
      301 tactgcatct cctatggtca gtgagcggat ggctgaaatg aaagatccgt gtctgagcgt
      361 gctcacaaag ccggtttcat ttgccgagct gaaagccgga attgcacgct atctcaaata
      421 agacgtcagc cgatccgcgg ttgtcctttt tttagtccgt gaattcagta gtaattcctg
      481 tacttcacgt ccgggggttt tttgcggtct taacaggtca ttctgacaat aatccaccgg
      541 gacagggatc attattgccg gtgaaagagg ggcattgcgc cagagtcctg gtctaaggtt
      601 gattattaaa aaaaagcttc ttttggttct gcagaaacgc acatgaactt acatgttcac
      661 acccgaacca tgaaaattgt tttctctatg atgtaccccc gcctcagggc ctctttttag
      721 gcacagcgga aaaggagttt ttatagtttt tcagttactg atccgccgcg ggggcgcccc
      781 accgggggcg gcggtgttca tcgtaactgt agaatagaga tgataatccc cacaccatac
      841 atgaatgatt tgattttcat gggaattcct ttaccagtgt gcggaattaa aaaacacaga
      901 tgcattagag gagggggaca gtgcctggca cctcggtagc gccccttcgc tgtgcgacag
      961 tgctgctatt accttctcag ggagttatcg catctggggg gagccgcaac ggcaggagtg
     1021 aaaccccgtg atcgttcagg ttttttaaaa aaaacagttc taccgatttg ttcatttagt
     1081 atcgcaaaaa tgccaggatt tgtgatagtc aggcgccatt tggttcaaca ccggggtacc
     1141 ggataaaaaa attatttcag atataccggg ccttctttga aagtttccgg ggcgtataga
     1201 gaggcctgaa cggtttgcct tcagtatcct tgtgaactgc ttcgatcagc tcatccatgg
     1261 tgagctgctc ttttgcgggt ttgttgggct gcgatttcct tcggatagta acagtgagtt
     1321 ttctgctggc agcttcttca tcaccgacaa cgactacaaa gggtatccag tccattccgg
     1381 cctccctgac tttcttaccc acgctttctt cccggtcatc gacatcacag cggatttgtg
     1441 cggtatttat ttgagcacag acctcttctg caaaagcccc atgacgctcc gcgacaggta
     1501 caacacgcac ctgcaccggt gaaagccagg tgggcagtga ggggacagcc tgtgttgaga
     1561 ggttttccag aatggcgcag atcacgcgtt caatgctgcc cgtgggagaa caatgaagga
     1621 taggagggtg aactacagta ccatcatccg tatggtattt tatattgaac cgggtcgagc
     1681 tctcgacatc gatctggacg gtggggttct cgatcggtcg catctggccg tctatggcag
     1741 cgagatcgat cttggcaacc cagtaatgga cgcggtcgga caggatctcg atgagcatcg
     1801 ggcagtcaga ttccttcaca atctttttta cccaggcttc gttctgggcg tagaagtcct
     1861 ttgtgcagcg gaatgcagcg accagctttg tctcaaagtc cttcccggtc tgccagccca
     1921 tggcaagctg ctcttcaaaa catttcagcg cttcgggcat atctgtgcag agagagtgca
     1981 tatcgggcat ggtaaatgca cggaggcgct taagcccgat cacttcccct ttctgttcgt
     2041 gccggaacga gtaggtggaa agttcataca tcttcatcgg gaggttgtgg ggtgagatgt
     2101 gcatgtcccg cataatggag aacataccga agcaggctgc aaaccgcagc atcatattcc
     2161 ggttgttgga cttgaaccgg tactgccgct cgccgaattt ctccgcatgt tcatagattg
     2221 cccggtcacc gagatcatac atgaccggtg tctccacggg agtaccgcca tattccagaa
     2281 ccagcctcag aacataatcg gacaggagat cacggatgat cttgcctttc ggcatccagc
     2341 gaaggcagcc cacatcgctc gccggctcgt agtccaccag ctcctttgag cgcatcaggc
     2401 cgacatgagc aggttcccca ccggttggaa ccgcgacccc gagttccttt ttgacaaggc
     2461 agccaaacgg tgaatcatca aggaactcct tataatcgtg ggatttccca tcaggagtca
     2521 tgacaaacca gtcgtgggta atctcagatt tttcagattt tgcagttcct ccttcaccgg
     2581 gcacgatcgt ctttgagagc tctgaaagag ggtggccttt gcaggagatc ttaaaggatt
     2641 tgtaccagcc aaaaggcgcc cgcttgacaa aaaacccttc ctgttcaaga cctgtcttga
     2701 gggcattgag tgtggcaaca gcagtttcgg gtgatgcaag atcactggag aggtgggcat
     2761 acgggtacac gacaatctta tcgacatgga cctgcccggc agtctttttt acctcgtcaa
     2821 ttgcctggag gacaactcct tgtaaatctt cctcgtcaat cgattccact gcacagaaaa
     2881 ccgtaagtac ttctgtctcc ctgtcagaga ggacagaaca gtcctctgcc atctttgtct
     2941 tcttttttgc ttcgtattcg atataatctg aatgaatgag aagaagtcgc attaattcat
     3001 ctccgatccg gtgggatcat tcgtataaaa tgcgcattga ttgtatatag tgttaatagt
     3061 atttttttaa aaaaaatgat ctttagtacc cgattttatc aaaagagggt gggtgaaaga
     3121 aaaaattatg gttcacgggt cctgtggata tagagagccg acccgataat tccgcagata
     3181 aaacctgccg ggagagccat gaccgggaaa ttccggacag gtccgccttc atctggcgga
     3241 gcggcataac ggatacacgc cacgttcagg actacatcat cataatcccc gtcgcccggt
     3301 accgccggat tttcccgggc accgaacatg tcttcaaacc ctacacgata cgatccatct
     3361 tccagcatag tcacgaccgc gtggtcaaac ccgtccccgc tggtctgatt ggaatagtac
     3421 gtgactgagc ctccttcgag gggactttta caatacagga caaccggttc gtccgatgta
     3481 caatgaccga gggatttgta tgtggctccc ggaactggag taagattttc ttgtgtgaag
     3541 cccaggctga ctggctgtga tgtttcaatc ccgaattcat tgttataacc ggcggttccg
     3601 gaaatatagg tgaccacaag atctcctgtg taagaaaaat gaatgggccc cgatgctgat
     3661 accgtgctga cagttgtgaa cacaagaaga attatcacga taatccaggc ttttttcata
     3721 ccctcaatca ccaaatggat aatattaaat gattgtatca atacttaata ttattgtaat
     3781 gcatcaatga accaccttta ttcttcccgg gtatacttcc taaacggc
//
LOCUS       DASRLV010000057         4865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_581951,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000057 DASRLV010000000
VERSION     DASRLV010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581951"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VFC43_02385"
     CDS             <1..704
                     /locus_tag="VFC43_02385"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HZK30068.1"
                     /translation="LEEGIDEVTFACDLYSLMVKEGHQGIIRFGMFNEMLLGQIGFGI
                     SSISPICVDTPGGVSGMHPSVPQMGCRERKLGKGDLVVIDIGCGYHGYQTDKTLSYMF
                     GRPIPDAAIQDHNRCVEIQDAMASLLKPGAIPSEIYRTIMAGIELSFLPNFMGFGDRK
                     VKFLGHGIGLWIDETPVIAEGFDEPLEEGMVFALEPKKGIPDVGLVGIENTFVVTPQG
                     GRPLTGKSRGLIEVY"
     gene            complement(931..1530)
                     /locus_tag="VFC43_02390"
     CDS             complement(931..1530)
                     /locus_tag="VFC43_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleic acid-binding protein"
                     /protein_id="HZK30069.1"
                     /translation="MSLNARDSLRKDGVFEREPARRVFAAELRECRYQFKESEDEKSP
                     TFVLLPSGERCNRIFLVGTLTEKQRQGDQNIFYRGRVVDPTGTFFIMAGSYQPEAMQQ
                     LAKIDTPAFVAVIGKPNLYQTPDGAFLVSVRVESITVVDKETRDLWVLDTAARTLDRI
                     EAMATGQSVDIVKAQEQYPSIDLAVFRKMAYDALAQIKI"
     gene            complement(1527..2771)
                     /locus_tag="VFC43_02395"
     CDS             complement(1527..2771)
                     /locus_tag="VFC43_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HZK30070.1"
                     /translation="MDFFEAAERISRKFSQKGTEVDPKKIEGKLRRLVNEFGVQPSEA
                     ERSVNNELAKEFNLPAMGTGGGGKSSGAAEIKKIADVAPGEWVTVEGKVVAISAPASP
                     AIAQSGIISDESGAIRFVAWAKSNAPAMSVDSWYKIESAVVDEFKGTASLKIHSGTTV
                     KESAIDGPLIPSTVPVLNLHTGVGSVRVKVVQEWDVSHERMLQSGLLADETGSVKFVI
                     WKEPGKEKLTVGAVYNIFYALIDEFNGRLSLNLSTATIMQDDGDILVSGGDVTARGAM
                     VHVAPGSGIIKRCPVEGCNRALSRQNYCPVHEIQPKFHYDLRIKGWLDDGLKTHNILL
                     QRDVVESLTGITLEAAQEIAENNPLGMDEVFLRMRDAILGRYISCQGREIDNRLLVNE
                     CKRLTYESGEHTALLNRSGSVS"
     gene            2892..3086
                     /locus_tag="VFC43_02400"
     CDS             2892..3086
                     /locus_tag="VFC43_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HZK30071.1"
                     /translation="MVDITADSTIYDLLKAKPEATEALFKFGMGCVGCAIARGETIRE
                     AAEAHGIPIAELLSALGIKE"
     gene            complement(3087..3971)
                     /locus_tag="VFC43_02405"
     CDS             complement(3087..3971)
                     /locus_tag="VFC43_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HZK30072.1"
                     /translation="MRYHFNTSSLFIRGSFRAASTGVSGGIRSVSTLINHTVSAGWSH
                     EDPKKELEFVAASAGISHDFFGLLTAVPVQQCCVLQYDSVTLFITAGIRREPPSNAGT
                     INIIIYSNEGLEDSALLETIMVATEAKAEALQAMGLPVTGTPTDAVIAAGEGEIKYSY
                     AGRLTETGRRVRETVLFGIPEAIKLHDAPKKSPHLSFFIFSRFQGNHWVKWSPDNCPY
                     FPCHHPGQRCDFCYCPFYPCREEGLGEWTQSSHGGKVWNCARCTLPHEPEVADYLKKF
                     PVASLAELKNLYRKKENQ"
     gene            complement(3968..4330)
                     /gene="nrdD"
                     /locus_tag="VFC43_02410"
     CDS             complement(3968..4330)
                     /gene="nrdD"
                     /locus_tag="VFC43_02410"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HZK30073.1"
                     /translation="MDWTAEQRTLPLKYKRLDDIPEKERKYKCHTCHLIVDEKPCPKC
                     GETHLEIMCPLDHCHCTHEIISGIEYCPLCGKAVCPECGSHDVTQISRVTGYLQDVSG
                     WNASKQQELKDRMRYSVA"
     gene            complement(4456..>4865)
                     /locus_tag="VFC43_02415"
     CDS             complement(4456..>4865)
                     /locus_tag="VFC43_02415"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase / HUMPS
                     family protein"
                     /protein_id="HZK30074.1"
                     /translation="AGFSSIICHGFTGKDAVQACVDASSDYGGACFVVAEMSHPGATE
                     FFQGGAAERIAHLAMECGADGIIAPATRPDRVNVLRHIVGNRKILSPGIGAQGGDADV
                     VARLVDGIIVGRAIFEADNPGNAAMGFARVRSV"
BASE COUNT         1136 a         1270 c         1245 g         1214 t
ORIGIN      
        1 tgctcgagga aggaattgat gaggtcacgt ttgcctgcga cctgtactcg ctgatggtaa
       61 aggaaggtca ccagggcatc atccggttcg gcatgttcaa cgagatgctg cttggccaga
      121 tcgggtttgg catcagctcc atcagtccca tctgtgtgga tactcccggc ggtgtctctg
      181 gtatgcaccc gtcagttccg cagatgggct gccgggaacg gaagctgggg aagggtgacc
      241 ttgtcgtgat tgacatcggg tgtgggtatc acggatacca gactgacaag accctgtctt
      301 acatgtttgg caggccgatt ccggatgctg caatacagga ccataaccgc tgcgtggaga
      361 tccaggatgc gatggcatcc ctgctgaaac cgggagccat tccctcagag atctaccgga
      421 ctatcatggc aggtattgaa ctgtccttcc tgcccaattt catgggtttt ggcgatcgca
      481 aggtgaagtt cctcgggcat ggcatcggtc tctggatcga tgaaacgccg gttatcgcgg
      541 aaggattcga tgagccgctc gaggaaggga tggtctttgc ccttgaaccg aaaaaaggga
      601 tcccggatgt cggccttgtt ggtatcgaga atacctttgt cgtgaccccg cagggtggtc
      661 ggccgcttac cgggaagagc aggggactca tcgaggtata ctgatgggca ttatcgtatc
      721 aaacgcggat gattgtgggg agcgcaggac agttttgtcc ggcataaaca gcttactttt
      781 ttgccagttt aggctccata ggcatcggaa agtgatcgga atccgggaag ataaggtttg
      841 ctcgaataat caaggggaca ataaaccaaa gaatacggcg tatctttatg ccagtcagaa
      901 agggaatgcc cggcttgata atgaaaaaaa ttatattttt atctgtgcca gtgcgtcata
      961 cgccattttc cggaacactg ccagatcaat tgagggatac tgttcctgcg cttttactat
     1021 atccacggac tgacccgtag ccatcgcctc gatccgatcg agtgtccgtg cagcggtatc
     1081 caggacccag agatcgcgtg tttccttatc gacaacggtg atcgattcaa cccttacgga
     1141 gaccaggaat gccccgtccg gagtctggta aaggttgggt ttcccgataa cagcgacaaa
     1201 tgcgggagta tcaatttttg ccagctgctg cattgcttca ggctggtaac tgccagccat
     1261 aataaagaat gtaccggtag gatcgacaac tctccctcgg tagaagatgt tctggtctcc
     1321 ctgccgttgt ttttcagtca gggtcccgac aagaaagata cggttgcagc gttcaccgga
     1381 gggaagaagg acaaaggtgg gactcttttc gtcctcactc tccttgaact ggtaccggca
     1441 ttcgcgaagc tccgcggcaa aaacccttcg tgccggctca cgttcaaaga ccccgtcttt
     1501 tcgtaaagag tcacgtgcgt tcaggctcat gacacacttc cggaccggtt cagcagtgct
     1561 gtgtgctctc cgctctcata ggtcagccgt ttgcactcat tgaccagcag acggttatcg
     1621 atctctctcc cctggcagga gatataacgc ccgagtatcg catcccgcat cctgaggaac
     1681 acctcatcca tcccaagcgg gttattctct gcaatctcct gtgccgcttc aagcgtaata
     1741 ccggtaagtg actcaacaac atcccgctgg agcaggatgt tatgggtttt taagccgtca
     1801 tcgagccagc ccttgatccg caaatcgtaa tggaatttag gctggatttc gtgaaccggg
     1861 caatagttct gtcgtgaaag ggcacggttg catccttcga cgggacatcg cttgataatg
     1921 ccggagccgg gtgcaacatg caccatcgct ccccgtgctg tgacatcacc accactcaca
     1981 agaatgtcgc catcatcctg catgatcgta gcggtgctca ggtttaaaga gagccttccg
     2041 ttgaattcgt ctatcagagc atagaagata ttatagactg cacccaccgt gagtttttcc
     2101 ttgcctggct ccttccagat cacaaatttg acagaccctg tttcatccgc caaaagaccc
     2161 gactggagca ttctctcgtg ggaaacatcc cattcctgaa ccacctttac acggacactt
     2221 ccaacacccg tatgaagatt gagaacagga actgtacttg ggataagagg accgtcgatc
     2281 gcactttctt ttacggttgt acccgagtgg atcttcaggc tcgcagtgcc tttgaactca
     2341 tcaaccaccg cagactcaat tttataccac gagtcaacac tcatcgcagg tgcgttcgat
     2401 tttgcccatg caacaaaccg gatcgctccg gactcgtctg agataatccc gctctgggca
     2461 attgcaggtg atgcgggagc tgagattgcc acaaccttgc cctcgacagt gacccactca
     2521 ccgggagcaa catcagcaat tttttttatc tcggctgcgc cactgctttt tccaccaccc
     2581 ccggtaccca tcgcgggaag gttgaactct ttggccagtt cgttgtttac actgcgttcc
     2641 gcttcggatg gctggacgcc gaactcattg acgagacggc gtagcttgcc ttcgatcttc
     2701 ttgggatcga cctctgtccc tttctgggaa aattttcggg agattctttc tgctgcttcg
     2761 aaaaagtcca ttcatacatc tccagtacta catcggttct gatcatgcct atatattgcg
     2821 aagtatgctt ggtgaaagtg aaccgccgct atggagagat ttatgtttga taacaatgac
     2881 ttagtgatac tatggttgac attaccgctg acagcactat ctatgatctc ttaaaagcga
     2941 aacccgaggc aactgaggca ctattcaaat tcggtatggg gtgtgtcggg tgcgccattg
     3001 cgcgtggcga gactatccgc gaggctgccg aagcccacgg tattcccatt gcagagcttc
     3061 tttctgcgct cggcataaaa gagtaattat tgattttctt ttttacgata caggtttttc
     3121 agctcagcaa gtgaggcaac ggggaatttt tttaagtagt ccgcaacttc aggttcgtgg
     3181 ggcagtgtgc accgtgcaca gttccagact tttccgccat gggaactctg tgtccattcc
     3241 ccaagcccct cctccctgca ggggtaaaac gggcaataac aaaaatcaca ccgctggccg
     3301 gggtggtggc agggaaagta cgggcagtta tccggtgacc attttaccca gtggtttccc
     3361 tggaaacggc tgaaaataaa aaacgacagg tggggggact ttttgggggc atcatgaagt
     3421 ttaatggctt ccggtatacc aaacaggacc gtttcacgga cacgacgacc cgtctctgta
     3481 agcctcccgg cataagagta ttttatttcc ccttcgcctg cagcgataac agcatcggta
     3541 ggagtccctg tcaccggcag ccccattgcc tggagtgcct cggcctttgc ttcagtagcg
     3601 accataatgg tttcaagcag tgcagagtct tccaacccct cattgctgta tattatgata
     3661 ttgatagtgc cggcatttga aggcggttcc cggcgaattc ctgcagtaat aaatagagtt
     3721 acagaatcgt actggagcac acagcactgc tgaaccggga ccgcggtgag cagtccaaaa
     3781 aaatcatggc tgataccggc actggcggcc acgaattcca gttctttttt cgggtcctcg
     3841 tgactccagc ctgcagaaac agtatggttt ataagggtag aaacggaacg aatgccgccg
     3901 gagacaccgg tgcttgcagc ccggaatgac ccccggataa aaagggaact ggtattaaaa
     3961 tggtatctca tgcgacagaa tagcgcattc ggtctttgag ttcctgctgt ttgctggcat
     4021 tccagcctga aacgtcctga aggtagccgg ttaccctgct gatctgggtg acgtcatgag
     4081 atccgcactc cgggcagaca gcttttccgc aaagcgggca atactcgata cctgatatga
     4141 tctcgtgggt gcagtggcag tggtcaagcg ggcacatgat ctcaaggtgc gtctcgccgc
     4201 atttcgggca cggtttttcg tcgacgatga ggtgacacgt gtggcacttg tatttgcgct
     4261 ccttttccgg aatatcgtcc agtcttttgt atttgagagg caatgttcgt tgttctgcag
     4321 tccaatccat actataacat tgtacaagtc cttaataaat atttagaccc ttttagttca
     4381 atgaacggtc aatatctgta taatcagccg cgcatatccg gatctggtca cagaatggag
     4441 gttatttata tatttttaca ccgaacgaac tcgggcaaaa cccattgcag cgttgcccgg
     4501 gttgtctgcc tcaaagatcg cgcgacccac aataattcca tcaacgagcc ttgcgaccac
     4561 atcggcatcc cctccctgag caccgatacc cggtgagagg atcttccggt tgccgacgat
     4621 atgtcgcagt acgtttaccc ggtcaggacg ggtagcggga gcaatgatgc catctgcccc
     4681 acactccatc gcaagatgtg cgatacgctc cgcggcccct ccctggaaga attctgttgc
     4741 tcccgggtgg ctcatctctg caacaacgaa acacgcaccc ccgtagtcgg atgaagcatc
     4801 aacacatgcc tgtacggcat ctttcccggt gaaaccgtgg catatgatag atgaaaaacc
     4861 tgcat
//
LOCUS       DASRLV010000058        12121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_582502,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000058 DASRLV010000000
VERSION     DASRLV010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 12121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12121
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582502"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1144
                     /locus_tag="VFC43_02420"
     CDS             173..1144
                     /locus_tag="VFC43_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZK30075.1"
                     /translation="MNEKKGSYLLVILSIVAVFMLAFAAGCTGNGTQTTQVPTASPTV
                     IATTQAPASTTPAAVMTIPPVSTGQKQTIKISGSTTVLPIVQKAADQYMAAHPDADIQ
                     ISGGGSGVGIQAIGAKTVDIGMSSREVTKDEMKKYPDFVVTSVAQDGIAVVVNPVNEI
                     QYITLDQIKGIYLGSITKWNQITGANVPDSNNQIVIIGRDSASGTRTYFDETILLKAT
                     PAKTMLEKNSNGAVLQTVAQTPGSIGYVSIWFVSKDVKALPVWYNAQKFVAPTIDNVK
                     TKTYPISRDLYVITNGKPAGLAGDFIRYILSPEGQKIVADEGYVTLG"
     gene            1197..3152
                     /gene="pstA"
                     /locus_tag="VFC43_02425"
     CDS             1197..3152
                     /gene="pstA"
                     /locus_tag="VFC43_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HZK30076.1"
                     /translation="MKFMKKMNGVLNASESTARLSFVKEQAIKTVFFFTAFFAVIVVV
                     FILLFLVRDGYVIFEKVGVLTFLSGATWAPTAVEPLYGIFPLIVGTLLVTIGAMIFAV
                     PLSIGCAIYISELASPRVKQVLKPAVELLTGIPSVVYGFFGLIVLTNFIRVTFAIPTG
                     ETWLAASVLLGIMALPTIISVSEDAISSVPHEYKEGSLAIGATRWQTISQVLVPAALS
                     GIAAAIILGVGRAIGETMAVLMVAGNAAIIPEPFWNILSPLRTLTATLGIEMGEVSVG
                     SDHYSALFGVALVLLIITLIINLTAVAILRHLKGGMTKTTSKKPLVSLHVKESFVRSA
                     SILGVLMVLVFLLIAAPLWLAVLIVLACGVWYFGRNMLSRYHIEMISFGLIMAATVIV
                     LAILVIILQDIIINGLPALSWEFLTQPPKDLGRAGGIFPAIIGTLYLVLGAIAIALPL
                     GVGAAIYLVEYTREGRITRLIRTGVDLLNGTPSIVFGLFGFAFIVLYLNIGVSMLAGQ
                     ITLALMVLPTVIRTTEETLKNIPQSLREGSLALGATRWQTISRVVLPPAVPGIVTGAI
                     LSIGRAAGETAPIMFTAVIFSSRFLPDSVFEPVMALPYHLFILATNVPGASTNKYGTA
                     LVLLLLVIGFYAVAIIVRNHFQKKSRG"
     gene            3156..3917
                     /gene="pstB"
                     /locus_tag="VFC43_02430"
     CDS             3156..3917
                     /gene="pstB"
                     /locus_tag="VFC43_02430"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HZK30077.1"
                     /translation="MSESAIITTNNVNLWYNEKRALESVSMTIPKNKVTALIGPSGCG
                     KSTLLRCFNRLNDLIDHVKITGEIMLDDENIHATGTDVVMLRKKVGMVFQKPNPFPKS
                     IYENVAYGPRVHGVKDKGELDKIVEQSLRHAAIWDEVKDRLNDSALGLSGGQQQRLCI
                     ARTLAVEPEVILMDEPCSALDPIATAKIENLIELLKKEYTVIIVTHNMQQAARVSDYT
                     GFMYLGKLIECGKTVQIFEHPKEELTENYITGRFG"
     gene            3927..4577
                     /gene="phoU"
                     /locus_tag="VFC43_02435"
     CDS             3927..4577
                     /gene="phoU"
                     /locus_tag="VFC43_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HZK30078.1"
                     /translation="MAEKFHTELKNLKAETLEMAHLGRFMLRNAVDALIRQDKELAAS
                     VVARKEEIHQMEVRLEEHCYQLIALNQPMAKDMRVIACTLKVITASLRIGRYGKVIAN
                     IVKKISDQPHIANLMTIPHMADLVIEMIDDAIEAYEKDSLAGISDFSARDDTIDSLRE
                     SIFREGITYMMEDPKSITRCTNYILVARYLERCADHACKIAENVHYMETGERIEIH"
     gene            4595..5041
                     /locus_tag="VFC43_02440"
     CDS             4595..5041
                     /locus_tag="VFC43_02440"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HZK30079.1"
                     /translation="MDREKKLVLFICTHNSARSQMAEGYLRARYGNLFDVRSAGTEVR
                     EVHPLAVAVMEEIGIDISGHRSKLIDEFFDRGADIVVTVCDSAHQVCPFFPGAKTTIH
                     AGFPDPSACVGTPDECHVQFRRVRDAVITWIDTTLVPEYGNNVPPS"
     gene            5317..5544
                     /locus_tag="VFC43_02445"
     CDS             5317..5544
                     /locus_tag="VFC43_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30080.1"
                     /translation="MLDTKNEILYRECRFDQKKARHIRSMGSILAAMTLMVNKLHWES
                     GASRVADMCTDARGFTYQTVRSCDLDRICDK"
     gene            5551..5751
                     /locus_tag="VFC43_02450"
     CDS             5551..5751
                     /locus_tag="VFC43_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30081.1"
                     /translation="MMSFSKVRKSPVRDTGFLSSREHMETVPMHPDDFEAHLAWIMAK
                     RFHELVLNETFPEKSVYYRIVG"
     gene            6030..6680
                     /gene="phoU"
                     /locus_tag="VFC43_02455"
     CDS             6030..6680
                     /gene="phoU"
                     /locus_tag="VFC43_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HZK30082.1"
                     /translation="MTDKFQSELLDLRRKSLEMAHLGRTMLRSAVDALIRHDPSLADS
                     VITRKEEIRQSEIGLEEHCYRLIALYQPMAKDMRNIVCTLKIIHASLRIGRYGMEIAK
                     VVREISENPHHANVMSIPHMGDLVIEMVDGAIRAYETDDLSLIRDFSSRDDSVDALRH
                     SIFREGITHMMEDPKNISRCTHYIMVARYLERCGDHACEIAENVYYMETGERIEIK"
     gene            6882..7256
                     /locus_tag="VFC43_02460"
     CDS             6882..7256
                     /locus_tag="VFC43_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30083.1"
                     /translation="MAESQFFPISPEMIALASETVSRRVSRLPFERNGVLVTEELISV
                     VMECLNAEFKKVLPLKTPQNAGPETQRGLDHCIEERVRIPQKTVVPVLAEVLCSAGIT
                     ETTEIMDRISHRPYKGIRLLPS"
     gene            7281..8261
                     /locus_tag="VFC43_02465"
     CDS             7281..8261
                     /locus_tag="VFC43_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30084.1"
                     /translation="MPALSPVVQGTNHEPVNASWTNKCPVCRTGILTRITGKQLFGIP
                     RTDFYEECTHCGAKFIPVGTQFRLVSIARIRDPLWKKHLDETYPPETWAALARGSGMI
                     PGSKPPVEVRVKNNDKLPPKSPAGGLTLTRDGSLVVPVLEKTLYFKPVKLQFSGGLRE
                     NVFARAQRPLDEILINPRFSHLVPAVNAKYSRCLPMQSGLFLGQLKERRDPFYREFLN
                     PYGDEKFCTFRLDGPQERDKKGVLIVVVNREIYHAVNCLSSFGAILNETYGRILPEDC
                     FLSGDPTRCRINALIGNNRKETGIYVHTSESEDDRIHITGILTRGISPDI"
     gene            8366..9742
                     /locus_tag="VFC43_02470"
     CDS             8366..9742
                     /locus_tag="VFC43_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HZK30085.1"
                     /translation="MKCPHCGMNIRDNIEICGYCGGKIEQESETSPSGTGRPEGSGGS
                     AGQKGAGTKASGSLHQESESDEEEEGGISVYLQPGEQVLIGSLNIAVKKFSFHAYLTN
                     QRIFLIDTQEKKVKVTAKDIPRESIVGSSLEYSESSDPLLVLSLKSTDDEIKTMKLIF
                     IQNGLDRSDEIDEWITLLKEHAPLKKIPALAEKELPEEKEEQPPVPKEKPVKKQELQP
                     KRKPGKDLEKQPPVKRHVVFHKMPEEVPDVEEPRPQRRSTVREVPETEKKPETEHEPS
                     YYEISPARRGDTQPLRKREVQSAMKVAMKNAMQPLKPSSLQSVKRPVVTERASAPVKE
                     PEPEDAEGIGRQADLREPAVADERNELPPFCQNCGKKIPPAANFCPGCGTKLGAYKAG
                     SPEWIPPPEKKTAHHEPPAREKRAPPRDEESDEEEKEDQAPVPTKPPAKKGPKGSEMT
                     ILHKFLRR"
     gene            10354..11556
                     /locus_tag="VFC43_02475"
     CDS             10354..11556
                     /locus_tag="VFC43_02475"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HZK30086.1"
                     /translation="MKRNIQIEALAQIPLEKQQIELVERKCLGHPDSMADGIAESISR
                     ALCKEYLEEFGAVLHHNTDQGEIVAGESSPKFGGGKIIRPIFILIDGRATKRFNDVSI
                     PTDAIAVEATRNYLRTHFKTLNMERDVIMDCRLGTGSTDLRDVFKPHNGRVPRANDTS
                     FGVGHAPFSDVENIIRSVSGYIDEKLRPKYPAIGQDIKIMGLRDGNTITLTVACAIVD
                     RYCADIRQYQEYMEILKEQIGKVAKKSTKRKVEVHVNTGDDIKNGSIYLTVTGTSAEM
                     GDDGSVGRGNRCNGLITPNRPMSMEATSGKNPINHVGKIYNLLSTQIATDCVAQVEGI
                     EEMYVRLLSQIGQPIDRPLVASVQVLPASGVKMQKIKGEIDGIVDHWLENVSAITEKV
                     IRGELKTF"
     gene            11563..>12121
                     /gene="hisG"
                     /locus_tag="VFC43_02480"
     CDS             11563..>12121
                     /gene="hisG"
                     /locus_tag="VFC43_02480"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HZK30087.1"
                     /translation="MAKQEKNGRKTSEPTPVVRLAIPNKGRIAAPIMDLVEKSGLHLP
                     EIGERRLITKTLDPHVEILFARPVDIPEYVATGAADIGITGHDMVVERESDVEEILDL
                     QSGKAKLVLAVHEDSAITSVKQLAGMKVATEFPVITKAYFAKHKVKVNVVLVGGACEA
                     TPHLGIADAIVDLSSSGTTLKTNRLK"
BASE COUNT         3262 a         3002 c         2978 g         2879 t
ORIGIN      
        1 ggaagctccc ccggttacct gtactcgccg tatctccatt atcctcaccg gtatgactat
       61 agaaaactga tttctctatt atactctatc tgtctatttc gtatatatat actagtatag
      121 tttatggcga gaagatgtat ataacgcccg gtaaaatgga tgatagaaca ccatgaatga
      181 aaaaaaggga tcatatttat tggtcatcct gagcatcgtc gcagtattca tgctggcgtt
      241 tgcggcaggt tgtacaggaa acggaacaca gactacgcag gtcccgactg catcccccac
      301 agttattgca accacgcagg cacccgcatc aactactcca gccgcggtaa tgactatacc
      361 cccggtctca accggccaga agcagaccat taagatcagt ggttcgacaa ccgtcctgcc
      421 aattgtccag aaggcagctg accagtatat ggcagctcac ccggatgcag acatccagat
      481 atccggtggt ggatcgggtg ttggtatcca ggcgattggt gcaaagactg ttgatatcgg
      541 tatgtcatcc cgtgaagtca ccaaagatga aatgaaaaag tatcccgact ttgttgttac
      601 ttcagttgca caggacggca ttgctgtcgt cgtgaacccg gtaaacgaga tccagtacat
      661 tacccttgac cagattaaag gaatatacct tggttccatc actaaatgga accagatcac
      721 cggcgcgaat gtgccagact ccaacaacca gattgtgatc atcggccgtg acagcgcgtc
      781 cggtacccgc acgtattttg atgaaactat cctgctcaaa gcaacgccgg ccaagaccat
      841 gctggaaaag aattccaacg gggcagttct ccagacggtc gcacagaccc ccggttcaat
      901 cggatatgtt tcgatctggt tcgtcagcaa ggatgtcaaa gcccttcctg tctggtacaa
      961 tgcccagaag ttcgtggcac caacgatcga taacgtaaaa accaagacat acccgatctc
     1021 ccgtgacctg tatgtgatca ccaacggcaa acccgccgga ctagccggcg acttcatcag
     1081 gtatatcctc agcccggagg gccagaagat tgttgctgat gaaggctacg tgaccctcgg
     1141 ttgaacgtat cagacaaaag gaacatcaca ttttttcttt cccggaggtg tgaatgatga
     1201 agttcatgaa aaaaatgaat ggggtcctga acgcttccga aagtactgcc cgcttatcat
     1261 ttgtaaaaga gcaggcaatc aaaaccgtat tcttcttcac agccttcttt gccgttattg
     1321 ttgtcgtatt tatcctactg ttcttagtac gggacgggta cgtgatcttt gaaaaggtcg
     1381 gagtacttac gtttctctca ggtgcaacct gggccccgac tgcggtcgaa ccgctctatg
     1441 gtatcttccc gcttattgtc ggtactctcc ttgtgactat cggggcaatg atctttgctg
     1501 tcccgctttc tatcggctgt gcaatctata tctccgagct cgcttctcca cgggtaaaac
     1561 aagtattgaa accggcagtt gaactgctca ccggtatccc ttccgtggta tacgggttct
     1621 tcgggctcat tgtccttacc aattttatcc gggtgacctt tgccattccc acaggcgaga
     1681 cttggctggc agcttcggtc ctgctcggga tcatggcact tcccacgatc atcagtgttt
     1741 ctgaggatgc gatcagttcg gttcctcatg agtataagga agggtcgctt gctatcgggg
     1801 caactaggtg gcagactatc agccaggtcc ttgtaccggc agcactttcc ggtatcgctg
     1861 ctgcaattat cctcggggtt gggagagcaa ttggggagac catggctgtc ctgatggttg
     1921 ccggaaacgc ggcgatcatt ccggagcctt tctggaatat cctctcaccg ttaaggaccc
     1981 ttactgcgac actgggcatt gaaatgggcg aggtttctgt cggaagtgat cactacagcg
     2041 ctttgttcgg tgttgcttta gtcctgctaa ttatcacgct tatcatcaac cttactgctg
     2101 ttgcaatcct gcgccatctt aagggtggaa tgacaaaaac gacctcgaaa aaaccacttg
     2161 tatccctaca cgttaaggaa tccttcgtac ggtccgccag catacttggc gttttgatgg
     2221 tacttgtttt cctcctcata gctgctcctt tgtggcttgc cgtgctcatc gttcttgcct
     2281 gcggtgtatg gtacttcggc aggaacatgc tttcaaggta ccatatcgag atgatctcat
     2341 ttgggctgat tatggcagca acggtcattg tgctcgccat ccttgtcatt attctccagg
     2401 atatcatcat taacggtctg ccggcactct cctgggaatt cttaacccag ccgcccaagg
     2461 atctcggcag ggcaggagga atttttccgg caattatcgg tacattgtat ctcgtcctcg
     2521 gtgcgatagc gattgcgtta cctctcggcg tgggggcggc aatttatctt gtcgaataca
     2581 cgcgggaagg acgtatcacc cggctcatca ggaccggtgt tgacttgctc aatggtaccc
     2641 cgtctatcgt ctttggcctg ttcgggtttg cctttatcgt gctgtacctc aatattggtg
     2701 tctcgatgct tgccggacaa atcacccttg ccctgatggt cctgcccacc gttatccgca
     2761 caactgaaga aacattaaag aatatcccgc agtctttgcg ggaaggaagt cttgcactgg
     2821 gtgctacccg gtggcagacg atcagtaggg tagtcctgcc cccggcagta ccgggtatcg
     2881 tgaccggagc gatcctctcg atcggaagag cagccgggga aactgccccg atcatgttta
     2941 cggcagttat cttctcgtca agattcctcc ctgattcagt ctttgagccg gtgatggcac
     3001 taccctatca cctctttatt ctggcaacca acgtacccgg cgcatcgacc aacaaatacg
     3061 gtactgcact tgtcctttta ctcttagtca ttggatttta tgctgtcgca atcatcgtac
     3121 gaaatcattt ccagaaaaaa tcacggggat aaaccatgtc tgagtccgca attattacca
     3181 caaataatgt aaatctctgg tataatgaga aacgtgccct tgaatccgtt tccatgacca
     3241 ttccgaaaaa taaggtgacc gccctcatcg gtccctcagg ctgcgggaaa tcaacactgc
     3301 tccggtgttt caaccggctg aatgacctta tcgatcacgt aaaaattacc ggcgagatca
     3361 tgctcgatga tgaaaacatc cacgcaaccg gaactgatgt ggtcatgctc aggaaaaaag
     3421 tcggaatggt cttccagaag cccaacccgt ttcccaaatc catttacgag aatgtcgcat
     3481 atggcccccg ggtacatggg gtgaaggaca aaggcgagct cgataaaatt gttgaacaga
     3541 gcctgcgtca tgcagcgata tgggacgagg taaaagaccg gctgaatgat tcggcgcttg
     3601 gtctctcagg tggtcagcag cagcgcctgt gtatcgcccg cacactcgcg gttgagccgg
     3661 aggtgatcct gatggatgag ccctgctcgg cgctggaccc cattgcgaca gccaagatcg
     3721 aaaacctgat agagttactc aagaaggagt atacggtcat catcgtcacc cacaatatgc
     3781 agcaggcagc ccgggtgagc gattataccg gtttcatgta tcttggaaaa ttgattgaat
     3841 gcggaaaaac agtgcagatc ttcgaacatc caaaagagga gctgacggag aattatatca
     3901 ccgggcggtt cgggtaggga agaaccatgg cagaaaaatt tcacacggaa ctcaaaaact
     3961 taaaagctga aacactggaa atggctcacc tcgggcgatt catgttgcgg aatgcagtcg
     4021 acgcactcat ccgccaggat aaagaactgg cagcatcagt cgttgcacga aaagaagaga
     4081 ttcaccagat ggaagtccgg ctggaagaac actgttacca gcttattgcc ctgaaccagc
     4141 caatggctaa agacatgcgg gttattgcct gcacactcaa agtgattaca gcatcactcc
     4201 gtatcgggcg gtacgggaag gtaattgcga atatcgtgaa aaaaatttca gaccagccgc
     4261 acatcgcaaa ccttatgact atcccgcata tggcggacct ggtgattgaa atgattgacg
     4321 atgcaattga ggcatacgaa aaagattctc ttgctgggat ctcagatttt tcggcccggg
     4381 atgacaccat agattcactg cgtgaatcga tcttccgcga aggaattacc tacatgatgg
     4441 aagacccgaa gagtatcacc cgctgcacaa attatattct tgtcgcccgc tatctcgaac
     4501 gttgtgccga tcatgcctgc aagattgctg agaatgtgca ttacatggag accggtgagc
     4561 gcattgagat ccactaaccc ggggaattca tggaatggat agggaaaaga agctcgtgct
     4621 cttcatctgc acccacaatt cagcccgctc ccagatggcg gagggatacc tgcgggcgcg
     4681 ttacggcaac ctgttcgacg tgaggagtgc aggaactgaa gtccgggaag tgcacccgct
     4741 tgcggtggcc gtgatggaag agatcgggat tgatatctca gggcaccgct ccaagctcat
     4801 tgatgaattc tttgatcggg gtgctgacat tgtggtgacc gtctgcgata gcgcacatca
     4861 ggtttgcccg ttctttccgg gggcaaaaac gacaatccat gccggttttc ctgatccatc
     4921 ggcctgtgtg gggacccctg acgaatgtca cgtacagttc cgccgggtga gggatgctgt
     4981 catcacatgg attgacacga cccttgtgcc ggagtacgga aataacgtgc cgccttcgta
     5041 aacaggtata atagtataag gattcaggtt agctgcggcc aataatatag ataatatgta
     5101 aattctatat agaaaatatt ttaagtatat acttacagaa tgaagtatgt aaatgttcag
     5161 caggatcccg tttgaggtgc gcatgaaaac tgtttatcag gaaggaaccg gtaaaacccc
     5221 ccatgcaggg agaaggttca gatgacttcg ggaaacatga accacctcgg gatatctgaa
     5281 gataatccgt tcgaataatt caggaatctc ataaatatgc tcgatacgaa aaatgaaatt
     5341 ctgtaccggg agtgtcgttt tgaccagaaa aaagcccggc atatacgttc aatggggtcc
     5401 atccttgcag ccatgacatt gatggtcaac aagcttcatt gggagtccgg cgcttcccgg
     5461 gttgccgata tgtgtactga tgcccggggg tttacctatc agacagtcag gtcgtgcgat
     5521 ttagaccgga tctgtgacaa atgaggagaa atgatgagtt tttcaaaagt aagaaaaagt
     5581 cctgtccgtg atactggttt tctctcatcc cgggaacaca tggagaccgt acctatgcac
     5641 ccggatgatt tcgaggcgca ccttgcctgg attatggcca aacggttcca cgagctggta
     5701 ctgaacgaaa cgtttccgga aaaatccgta tattaccgga tagttggata atgggttcat
     5761 tattcctgta cagaaaagag aaaaaggaaa aaactgcagg gatttttttt tgcaaatctg
     5821 aaacagttct ttagagaaga gcgtgcttct ggataaaata tcgtccgtaa ggagtactgt
     5881 attttatttt attctctatc gataccagat caatcaggat tttttgatgt gctcattacc
     5941 tacaatatga aacacggatc ccccgtcaac ctttatacaa atcaccgcaa acacactaac
     6001 ctgcattata tccgatcaag ggagggtaca tgacagataa attccaaagt gagctcctgg
     6061 atctaaggcg aaaatctctt gaaatggcac accttgggcg caccatgctc cgttctgccg
     6121 ttgatgctct tatccgacac gatccttcac tcgcggattc agtcattacc agaaaagaag
     6181 aaatccgtca gtctgaaatt ggactggaag aacattgtta ccgccttata gccctctatc
     6241 agccaatggc aaaggatatg cggaacatcg tctgcacatt aaaaatcatt catgcgtctc
     6301 tacgtatcgg gcgatatggc atggagattg caaaagtcgt ccgggagatc tccgaaaatc
     6361 cccaccacgc taacgttatg agcatcccgc acatgggtga tcttgtcatc gaaatggtgg
     6421 atggtgcgat cagggcttat gagactgatg atctttcact catcagggat ttttcatccc
     6481 gggatgatag tgtcgatgca ttgcggcatt caattttccg ggagggaatt acccacatga
     6541 tggaagaccc gaaaaacatc tctcgatgta cgcactacat catggtcgcc cggtatcttg
     6601 aacgctgtgg ggatcatgcc tgcgaaattg ccgagaacgt ttattacatg gaaaccggcg
     6661 aaaggataga gattaagtga gattttacaa tcccttttcc gacatcattt actctgcttt
     6721 tttggtgaaa tatactacaa ggatgcaacc gcctcgttat tccctgactc cgtgatgcat
     6781 ggaatgagtt tctgttttac tcaagtggga aaaccacaaa tggtcctgaa gagctattct
     6841 ttatcctcca tgggtgacaa caatcaggaa caacacacac catggctgaa tcacagtttt
     6901 tccccatatc ccccgagatg atagcacttg cgagtgaaac ggtcagcagg cgcgtctcaa
     6961 gactcccctt cgagagaaat ggtgtcctgg tcaccgagga attgatcagc gttgtcatgg
     7021 aatgcctgaa tgccgaattc aaaaaagtcc tgcccctgaa gacaccccag aatgctggcc
     7081 cggaaaccca acggggactc gaccattgca ttgaagaacg ggtaagaatt ccgcaaaaga
     7141 cggttgtgcc ggtacttgcc gaagtcttgt gcagtgcagg tatcacagag acaaccgaga
     7201 tcatggaccg gatttctcac cggccctata aaggaatccg gctgttaccc tcctagacct
     7261 ggcatattgc ctcccccgga gtgcctgcgt taagccccgt tgtgcaggga accaatcatg
     7321 aaccggtgaa tgcatcctgg acaaacaagt gcccggtgtg ccgcacggga attttaaccc
     7381 gtattacggg aaaacagctg tttgggattc cccggacgga tttctatgaa gagtgcacgc
     7441 actgtggggc aaaattcatt cctgtcggca cgcagttccg gttggtctcc attgcccgta
     7501 tccgtgaccc tctctggaaa aaacacctcg acgagaccta tcctcccgaa acctgggcag
     7561 cactcgcgcg gggatccggt atgattccag gcagcaaacc tccagtggag gtccgtgtaa
     7621 aaaacaatga caagctgccg ccgaaatctc ctgcaggagg acttaccctc accagggatg
     7681 gctcgctggt ggtgccggtt ttggaaaaga ctctctattt caaaccggta aaactgcagt
     7741 tttccggtgg cctgagagaa aatgtttttg cccgggcaca aaggcctctt gatgaaatcc
     7801 tcataaatcc ccgtttcagt cacctggtcc ccgcggtgaa tgctaaatac tcccggtgcc
     7861 tccccatgca atccgggctc ttcttagggc agctcaaaga acgccgcgat cccttttacc
     7921 gggagtttct caatccctat ggtgatgaaa aattctgcac gttccggctg gacgggccgc
     7981 aggaacgaga taaaaaaggt gtcctgattg tcgttgtcaa ccgggagatc taccatgcag
     8041 tcaactgttt aagttcattc ggtgcaatcc tcaatgaaac ctatggccgc attctgcctg
     8101 aagactgctt cctgagtgga gatcccaccc ggtgcaggat aaatgcactc atcgggaata
     8161 accggaaaga gacgggaatc tacgttcata ccagcgaatc agaagatgac cggattcata
     8221 tcacgggaat tcttacgcgg ggtatttcac cagatatcta aaagaaaatt tcctgcggac
     8281 ccgaaccgaa cagaccagag aatttttctt atcatgtgag aacatatagc tatccagtct
     8341 gggtaccgga aaagggagat tactaatgaa atgccctcat tgcggaatga atatacggga
     8401 taatatcgag atctgcggct attgtggcgg aaaaattgaa caagaatcag aaacatctcc
     8461 gtctggaaca gggcggccgg aaggttccgg tggtagtgcg gggcagaaag gagcaggcac
     8521 aaaagcctcc ggatcactcc atcaggaatc cgaatctgat gaggaagagg aaggagggat
     8581 ttctgtatat ctgcaacccg gtgaacaggt cctcatcggg tcgttaaata ttgccgtaaa
     8641 aaaattctct tttcatgcat atcttaccaa ccagcgcatc tttttaatcg atacccagga
     8701 aaaaaaggtc aaggttactg caaaagatat cccccgtgag tcgatagtcg gaagctcgct
     8761 tgagtattcg gaaagttcgg atcccctcct ggttctctcc ctcaagtcaa ccgatgacga
     8821 aatcaagaca atgaaactta tcttcatcca gaacggtctg gaccgttccg atgagatcga
     8881 tgaatggatc acgctcttaa aagaacacgc cccgttaaaa aagatcccgg cgctggcaga
     8941 aaaagaactt cctgaagaaa aagaagaaca gccacctgtt ccaaaagaga aacccgtaaa
     9001 gaaacaggaa ctgcaaccaa aaagaaaacc cggaaaagac cttgaaaagc agcccccggt
     9061 caaaaggcat gtagtgttcc acaagatgcc agaagaggta ccagacgttg aagagccccg
     9121 tcctcaacgg cgttcaacgg tgagagaagt acctgaaacg gagaaaaaac cggaaacgga
     9181 acacgaaccc tcgtattatg aaatatcccc tgcaagaaga ggagataccc agccgctccg
     9241 caaacgcgag gtgcagtctg ccatgaaagt tgccatgaaa aatgcaatgc agcccctcaa
     9301 accctcctcc cttcagtctg tgaaacggcc ggtagtaaca gaacgggcat cggccccggt
     9361 taaagaacct gagccggagg atgctgaagg aattgggcgg caggcagatc tgcgggagcc
     9421 ggcagttgcc gatgaacgaa atgagctccc cccgttctgc cagaactgcg ggaagaaaat
     9481 cccgccggct gccaacttct gcccgggctg cgggacaaaa ctgggtgctt ataaagctgg
     9541 ttccccggaa tggataccgc cacccgaaaa gaaaacagca caccatgaac cacctgctcg
     9601 tgaaaaaagg gcaccccccc gtgatgagga gagcgacgag gaagagaagg aagatcaggc
     9661 cccggtccca accaaacctc ccgcaaagaa aggtccaaag ggcagcgaga tgaccatcct
     9721 ccacaaattc ctgagaagat aattttttta gctcttgaga aattattttt gaaaattctg
     9781 attctctttt tgggactgtg ccctttcgcg cccttggcat tcctcttgta aaaacggtat
     9841 tgtatctgct atcagccctg atttttcgta ggggcaatta ttttatcaca aaaaaatttc
     9901 ttatgaaaat tgtgttattc agtccattat gcgagaattt ttttttaacc ctcataacaa
     9961 ttgatacaat aattgccgcc gcccccaaag ggacgtcccc gaggcggatc ggtgactaaa
    10021 atttaaaaaa atttaagccc gccatacctc taaagagccc tgatgtgggg ttcctttgta
    10081 gttagaacga ttttcatagt cctggtgtga accggatcgg ttcatggctg tttctgcaga
    10141 gcatttttta attcctctca ttttttttaa aaacgtgagc atatctgcat tcttagtagg
    10201 gcctgattct attcttcacc acgttcgtcc gataaacgga aataatcatt ttaattactc
    10261 ccaatcataa aaaaaacaaa aactatggta aatgagaaat tgttatatga attccagtcc
    10321 cctttattag tacgcattgt tatgaggaaa tcaatgaaga gaaatatcca gattgaagca
    10381 ttagcccaga tccctttaga gaaacaacag attgaacttg ttgagcgcaa gtgtctcgga
    10441 cacccggaca gtatggccga tggcattgcc gagtctatca gcagggccct gtgcaaagaa
    10501 tatctcgaag agtttggagc cgtcctacac cacaataccg accagggtga gattgtagcc
    10561 ggggaatcca gccctaagtt cggtggcggc aagattatcc ggcccatctt tatcctgatc
    10621 gatggtcgtg caaccaagcg gttcaacgac gtatctatcc ctaccgatgc tatcgccgtt
    10681 gaagcgacac ggaattatct ccgcacccat ttcaagacgc tgaacatgga acgcgatgtc
    10741 atcatggact gccgtcttgg aacaggatcg acggatctcc gcgatgtatt caagccccat
    10801 aacgggagag tcccaagagc aaacgacaca tcatttggtg tcgggcatgc cccgttttcc
    10861 gatgttgaaa atattatccg gagtgtaagc ggttatatcg atgaaaaact gcggccaaag
    10921 tatcctgcga tcgggcagga tatcaaaatc atggggttgc gtgacggcaa taccatcacc
    10981 ctgacggttg cctgcgcgat cgtcgatcgg tactgtgcag atatccggca ataccaggaa
    11041 tacatggaga tcctcaaaga acagatcggc aaagtggcaa agaagagcac gaaacgcaag
    11101 gtcgaggtcc atgtgaacac gggtgatgac ataaagaacg ggagcattta tctgaccgtc
    11161 accggcacct ccgcggaaat gggtgatgat ggctcagttg gtcggggtaa ccgctgcaat
    11221 ggcctcataa ccccaaaccg cccgatgagc atggaagcaa cgagcggaaa gaacccgatc
    11281 aaccacgtcg gcaagatcta caacctcctc tcaacgcaga ttgccactga ttgtgttgcc
    11341 caggtggagg gtattgaaga gatgtatgtc cgccttctct cgcagatcgg acaacccatc
    11401 gaccgcccgc ttgtggcaag cgtgcaggta cttccggcat ccggtgtcaa aatgcagaag
    11461 atcaagggtg aaattgacgg gattgtcgat cactggcttg agaacgtatc cgccatcacc
    11521 gagaaagtca tacgcgggga actcaagact ttctgatacc ctatggcaaa gcaggaaaag
    11581 aacggccgga aaacgagtga acctacaccc gttgtgcggc tcgctatccc gaacaaaggg
    11641 aggatcgcag cacctatcat ggaccttgtg gagaagagcg ggctccacct tccggagata
    11701 ggagagcggc ggctgatcac aaaaacgctt gacccgcatg tcgagatcct gtttgcccgt
    11761 ccggtcgata tacctgaata tgtggcaaca ggggccgcag atatcgggat caccggccat
    11821 gatatggtag tcgaacggga gtccgacgta gaagagatac tggacctcca gtccgggaag
    11881 gcaaaactcg tgcttgccgt tcacgaggat tcagcgatta cttccgtaaa acagcttgcc
    11941 ggcatgaaag tcgctacgga atttcccgta attaccaaag cgtactttgc gaaacataag
    12001 gtaaaggtca acgttgtcct tgtgggcggg gcctgtgagg cgacaccgca cctcgggatt
    12061 gccgatgcaa tcgtggacct ttccagttca ggtacaacct taaagaccaa ccgtctcaag
    12121 g
//
LOCUS       DASRLV010000059         3760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_827409,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000059 DASRLV010000000
VERSION     DASRLV010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827409"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VFC43_02485"
     CDS             complement(<1..331)
                     /locus_tag="VFC43_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30088.1"
                     /translation="MTFEPVTRRLKTCALFCLLILCVTGGAHARTDGSQSNPEPAFYL
                     NFNEGSGNYALDASGNGNTGMIYGASRIESGGCGRAISLDGMNNFVEIPFSFQNHPTK
                     AITVSLWF"
     gene            446..886
                     /locus_tag="VFC43_02490"
     CDS             446..886
                     /locus_tag="VFC43_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxx-star family glutaredoxin-like
                     (seleno)protein"
                     /protein_id="HZK30089.1"
                     /translation="MRKVTVFSTKNCPYCHMAKAFLDKQGVHYESIDVGEDRDAAKKM
                     IDLSGQRGVPVITVDDEVIVGFDSQRLNELFGQPALGEIYDVLIIGAGPAGLTAGVYC
                     ARKMMNTIIISENIGGQALESWAIENYMGYRMIAGEDLMKKFEE"
     gene            902..1603
                     /locus_tag="VFC43_02495"
     CDS             902..1603
                     /locus_tag="VFC43_02495"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK30090.1"
                     /translation="MNLELDRIVKISKEDELFTIRTISERIFKAKTLILAQGNRPKKL
                     GVANEEQFLGRGLSICSTCDGPLFKGKRVAVVGGGNSALQTAVEMSEITQSVMLIVRS
                     SIRADPVYVKMLESKKNITLHLNSQVVSLHGEKFLSGITVKNEKGEEQKIDLDGVFIE
                     IGWLPNTDMLDGFLALNEKKEIIVDINCHTSMAGVFSAGDVTSVKGKQIIIAAGDGAK
                     AALEAHEYLLKAASR"
     gene            complement(1683..3152)
                     /gene="tfrB"
                     /locus_tag="VFC43_02500"
     CDS             complement(1683..3152)
                     /gene="tfrB"
                     /locus_tag="VFC43_02500"
                     /EC_number="1.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043830 - thiol-driven fumarate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (CoM/CoB) subunit TfrB"
                     /protein_id="HZK30091.1"
                     /translation="MKEITARIYRFDPENDTEPHFDSFTVAVHEGARILHVLHAIHET
                     LDPTLSYRYSCGSGQCGSCAVRVNGEPVLACMEEAKNNITIEPLHLAVKKDLVVDLLP
                     TLETIAFLKPADTMKMPTKSDIDLIKPLRDCIECLCCVSVCPAMDVTAFLGPTAMRQE
                     MRLALDPRDHGDRIVDAVRDGLFTCTSCQACWKACPKNIEIPSKAIEKLRALANRRGF
                     TLPKHQEVAALIKDTGRSVGRTEPTFMERVNTEVIEPLGPVKATMGFFIGCMYNMRLP
                     NTALDAIEVLRRNGIRIIIPKEQVCCGSPLIRTGQIAFLDTLMKRNINAFRFRNIDTV
                     MTMCAGCGSTLKNDYKTPFKVMDISEVLTKYGLEPPARLPIKATYHDPCHLLRGQGIS
                     SEPRSLVKQVVDLVEMPAICCGSGGGVRSGNPEEAAALGKRRGEEIKKSGAEIVISSC
                     PFCEFHIAGHTDKPVKNIATVLLEGYKEKDRKKTPGSIS"
     gene            complement(3149..>3760)
                     /locus_tag="VFC43_02505"
     CDS             complement(3149..>3760)
                     /locus_tag="VFC43_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZK30092.1"
                     /translation="APTAHHIMGGLRITTECRTTIPGLFACGEVAGGVHGANRLGGNA
                     LADTQVFGKRAGEFAGKTREQIKKVSENQIKQVQDILDSFHNGSENPAQIRTKLQKTM
                     WQGAGIFRNAIDLNKTLEIVHELSLVPLKAESPRNIAECCIVRNMCLTASLICRSALI
                     RTESRGAHVRVDIPQTYEARHSPFLHTFCSLSRQGMEKIPGAA"
BASE COUNT          984 a          863 c          894 g         1019 t
ORIGIN      
        1 aaaaccataa ggaaacagta attgcttttg tgggatgatt ctggaagctg aatggtattt
       61 cgacaaaatt attcatcccg tctaatgata tagccctccc gcaacctccg ctttcgatgc
      121 gtgatgcgcc gtaaatcatt cccgtgttac catttcccga agcatcgagg gcataattac
      181 cgctgccctc gttgaagttg agataaaagg caggttcagg attggattga gaaccgtcgg
      241 tccgggcatg cgcccctcct gtaacgcaaa ggatcagcag acaaaaaagg gcacaggttt
      301 tcagtctccg ggttacaggc tcaaaagtca tggtataggt aaaggtgtat tggggctcgt
      361 ccaaaaagaa agttgctatg agctgactta tctttcctgt tcccgtgtca ataaatttat
      421 aagaaacaac accattttcg aactgatgag aaaagttacc gtcttttcaa caaaaaattg
      481 tccttactgc cacatggcaa aggccttctt ggataaacaa ggcgttcatt atgaaagtat
      541 tgacgttggt gaagaccgcg atgcagcaaa aaaaatgata gacctttcag gtcagcgtgg
      601 tgttccggta attactgtcg atgatgaagt gattgtcggg tttgactcac agcgactgaa
      661 cgagcttttt ggtcaacctg ctttgggaga aatttatgat gtattgatta tcggtgccgg
      721 cccagccggc ctcacagcag gagtatattg tgccagaaaa atgatgaata cgatcattat
      781 cagtgaaaat atcggcgggc aggcactgga atcatgggct atcgaaaatt acatgggata
      841 tcgcatgatt gccggggaag atctgatgaa gaaatttgaa gagtaggtca ggactcttaa
      901 tatgaatctt gaactcgacc gtatcgtaaa aatttcaaaa gaagatgagc tgtttaccat
      961 tcggacaatt tcggaaagaa tattcaaagc aaaaaccctg attcttgccc aaggcaatcg
     1021 tccaaaaaaa ctcggtgtag caaacgagga acagtttctc gggcgcggtc tttcaatctg
     1081 ctcaacatgt gatggccccc tttttaaagg aaaacgggta gctgttgtgg gtggtgggaa
     1141 ttctgcgtta cagacagctg ttgaaatgag tgaaattacc caatccgtaa tgctcattgt
     1201 ccgaagcagc ataagagcgg acccggttta tgttaaaatg cttgagtcaa aaaagaatat
     1261 cactctccat ctgaattccc aggttgtttc tcttcatggg gagaagttcc tctcgggaat
     1321 cacagtaaaa aatgaaaaag gcgaagagca gaaaatcgat cttgatggtg tttttattga
     1381 gatcgggtgg ttgccgaata cagatatgct tgacggattc ctggcactga atgaaaaaaa
     1441 ggagatcatt gtggatatta actgccatac cagtatggcg ggtgtttttt ctgccggcga
     1501 tgtaacttca gtaaaaggca agcagatcat tattgcagcc ggtgatggag caaaagcagc
     1561 gcttgaagca catgaatatt tattaaaagc ggcttcccga taggaaaaac tatacctttt
     1621 tgcctgacat taaaaaaatc aataatgttt atagttgaaa ttcatgtatc cagaaatgaa
     1681 attcacgaaa tggatccagg agttttctta cggtcttttt ctttataccc ttcgagcagc
     1741 accgtcgcga tatttttgac gggcttatcc gtatgtccgg cgatatggaa ctcacagaac
     1801 gggcaggatg aaattacaat ttcagccccg ctctttttta tttcctcacc tctgcgtttc
     1861 ccgagagcag ccgcttcttc cggattaccg gaacgcactc cccctcccga tccgcaacag
     1921 atcgcaggca tttccacaag gtctactacc tgcttaacca gcgagcgggg ttcacttgag
     1981 ataccctgtc cacgcaggag gtgacatgga tcatgatatg ttgcttttat cggcaagcgt
     2041 gcaggaggtt ccaggccata ttttgtaagt acttctgaaa tgtccattac cttaaacggt
     2101 gttttataat cattcttcag cgtcgaacca catccggcac acatggtcat caccgtatcg
     2161 atgttccgga accggaatgc attgatattg cgtttcataa gcgtatcgag gaatgcaatt
     2221 tgtccggtcc ggatgagcgg tgaaccacag cagacttgtt cttttggtat aataatccgg
     2281 attccgttac gtctcagtac ttcaattgca tccagtgccg tattgggcag gcgcatatta
     2341 tacatacacc cgataaagaa tcccatcgtt gccttcacgg gacccaaagg ttcaatcacc
     2401 tctgtgttca cgcgttccat aaaagttggt tctgtacgtc cgaccgatcg tccggtatct
     2461 ttgataagtg ctgccacttc ctggtgtttg ggcagggtaa atccccgcct gtttgcgagt
     2521 gcccgtagct tttcgattgc cttagacgga atttcaatat ttttggggca tgctttccag
     2581 caggcctggc agctggtgca ggtaaacaga ccatctctca cagcgtcaac aatccggtcc
     2641 ccatggtccc ggggatcaag cgcaagccgc atctcctgtc tcatcgctgt aggtccgagg
     2701 aacgctgtaa catccattgc cgggcagacc gaaacgcagc acaggcattc aatgcaatcc
     2761 cgcagaggtt ttatcagatc gatatctgat tttgttggca ttttcatcgt atccgctggt
     2821 ttcagaaacg cgatcgtttc aagtgttggg agaagatcaa caacaagatc tttttttact
     2881 gccaggtgca gcggttctat agtgatgtta tttttagctt cttccataca tgcgaggacc
     2941 ggctcaccat ttacccgtac agcacagctc ccgcattgtc ccgagccgca gctgtaccga
     3001 taggaaagcg tcgggtccag cgtttcgtga atggcatgga gaacgtgaag gatacgggca
     3061 ccttcgtgga ctgcaacagt aaaactatcg aaatgcggct cggtatcatt ctcgggatca
     3121 aatcggtata tcctggcggt gatttctttc atgctgcccc cggaattttt tccataccct
     3181 gccttgagag cgagcagaac gtatggagga acggagagtg ccgggcttcg tatgtctggg
     3241 ggatatccac ccgaacatga gccccgcggg actcagttct gatcagggca ctccggcaaa
     3301 taagcgaagc agtcaggcac atattacgga caatgcagca ttcagcaata tttctgggag
     3361 attctgcttt taatggaaca agagacaatt catgaacaat ttccagtgtc ttattaaggt
     3421 ctattgcgtt tcggaagata cctgctccct gccacatggt cttctggagc tttgtcctta
     3481 tctgtgcagg gttctcgctg ccgttatgga acgaatcgag gatatcctga acctgtttta
     3541 tctggttttc actcactttt tttatctgtt ctcttgtttt gcccgcaaac tccccagccc
     3601 gtttcccaaa gacctgggta tccgcaagcg cattgcctcc aagacgattt gcaccatgca
     3661 caccacctgc gacttcaccg caggcaaaga gtcccggaat tgttgtccgg cattcggttg
     3721 tgattcgtaa cccgcccatg atatgatgcg ctgtgggggc
//
LOCUS       DASRLV010000060         7416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_752645,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000060 DASRLV010000000
VERSION     DASRLV010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 7416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7416
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752645"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VFC43_02510"
     CDS             <1..463
                     /locus_tag="VFC43_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobyrinic acid a,c-diamide synthase"
                     /protein_id="HZK30093.1"
                     /translation="MGGVSQAGGGCLCPEYTLLAAIFRHMRLLPDEVVLLDTPAGMEH
                     FGRAVADGFSCALVVADPSYNALSVAKECSLLARQLGINDSILVVNRVGSPAEADKIH
                     RTMDGGSGFFRTIELPFDPEFVFAEPAVSQLLSGDSEFIKKARVLTEIITN"
     gene            544..1749
                     /locus_tag="VFC43_02515"
     CDS             544..1749
                     /locus_tag="VFC43_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZK30094.1"
                     /translation="MNPVHPGQTPSIPPAAEGFFSRHPSRADHFTESVIREMTRLSLN
                     YNAINLAQGFPDFSCPVELKNAACDAVQEDINQYAITWGAQDLREALSARVKKYNGMT
                     FHSDTEITVTCGSTEAMMASMLAVIQPGDEVVVPEPFYENYGPDAQISGAVPRFVPLR
                     DDFSLDEEAWKSAFTQKTRALILNSPNNPTGKVFSKSELMFIAELCSDYDVVAITDEI
                     YEHILYDKKKHISLGSMEGMQDRTITIGSFSKTYSVTGWRVGYALADARITERIRKIH
                     DFLTVGAPAPLQHACVAALRLPETYYQELAAMYDRKRKILFAGLRKAGFRCELPEGAY
                     YIFTDISGFDMTDTEFARYLVEKIGIAAVPGSSFYQNGGQTRLRFTFSKKDETLVEAC
                     RRLGNINQV"
     gene            complement(1822..2244)
                     /locus_tag="VFC43_02520"
     CDS             complement(1822..2244)
                     /locus_tag="VFC43_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HZK30095.1"
                     /translation="MIAIQTGDVDIGALIDAAKKMGTGAVVVFDGVVRDDDITEMELE
                     AYEDVAIQELQKIAKDATEQFRLLHVDIIHRIGRLSVGENILIIIVAAGHRKEAYAGS
                     RFIIEGIKAGVPIWKKELTRDGGRWVPGDQRHGSGKPR"
     gene            complement(2257..2535)
                     /locus_tag="VFC43_02525"
     CDS             complement(2257..2535)
                     /locus_tag="VFC43_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HZK30096.1"
                     /translation="MTVKIRFFARFRELLGNDIITDVDAGKMFTPLITNIAQKNPEGY
                     AAIFDETGAFREFVILMKNGKRIEIADAAQTPVEDGDEIAVFPPVAGG"
     gene            complement(2532..3293)
                     /locus_tag="VFC43_02530"
     CDS             complement(2532..3293)
                     /locus_tag="VFC43_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HZK30097.1"
                     /translation="MVLSQREQERYSRQILSFGEEGQERLKKAHIFIAGAGGLGSPVA
                     IYLAVAGVGTITIVDMDTVDQTNLNRQILHFDRDIGRKKTASAQEKLEEINPDITVNA
                     IDVKIDTANAANLVGNADGIVDAMDNYPIRYLLNDVAIKKELPFFHGGIRGFYGQVTT
                     IIPGKTPCLKCIIPKAPPAEVFPVVGVTPGFIGMVQATEVLKYFLGIGELLTGRLMIW
                     DGLQARAEEIRVERNYGCEACGGLVTKTTILEMKK"
     gene            complement(3331..3765)
                     /gene="nifU"
                     /locus_tag="VFC43_02535"
     CDS             complement(3331..3765)
                     /gene="nifU"
                     /locus_tag="VFC43_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HZK30098.1"
                     /translation="MYSETVMDHFKNPRNVGEIENPDGVGEVGNPVCGDIMKIFLKIE
                     NNIVIDAKFKTFGCGAAIASSSMATELVRGKTLEDAWELSNKAVAEALDGLPPIKMHC
                     SVLAEEGIHKAINDYRVKKGLVPWVEKNPHSHEHDEESTCQH"
     gene            complement(3755..4954)
                     /gene="nifS"
                     /locus_tag="VFC43_02540"
     CDS             complement(3755..4954)
                     /gene="nifS"
                     /locus_tag="VFC43_02540"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HZK30099.1"
                     /translation="MEGQRTIYMDHAATTSVKPEVVDAMIPYFTKHFGNPSSLYGIAR
                     ASKKAIDTARMQTAKALGAEPDEIYFTSGGSESDNWAIKGVAFANRKRGNNIITTQIE
                     HHAVIHTCQFLKKEGFEVTYLPVDRYGLIDPVDLEKAITDKTILISIMYANNEIGTIE
                     PIAELGAIAREHGIYFHTDAVQVIGNIPLDMNAQNIDLLSLSAHKFYGPKGVGALYIK
                     KGVRIENLIHGGGQERKRRAGTENIAGIVGLGKAIEMATADIPGHNAKIRAMRDRLIR
                     TVLDKIPNSRLNGHPEKRLAGNVNISFEFIEGESILLWLDDEGICASTGSACTSGSLE
                     PSHVLLATGLPVEISHGSLRLTLGDANSEADVDVVLDVLPKVVTKLREMSPLYNKSGK
                     EGGCNVQ"
     gene            5162..5494
                     /locus_tag="VFC43_02545"
     CDS             5162..5494
                     /locus_tag="VFC43_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HZK30100.1"
                     /translation="MEAPCQKIVWDVLPAIRAAIAVELVRCGVSQVEAARMLEIAPSA
                     VSQYLSGKRGYRIEFEEDVKKSIAQLAQDLIEKKQINVIERICGICRQLRDDEGGCGN
                     PVSEHCGT"
     gene            5548..6345
                     /gene="larE"
                     /locus_tag="VFC43_02550"
     CDS             5548..6345
                     /gene="larE"
                     /locus_tag="VFC43_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HZK30101.1"
                     /translation="MTHTTKIQLLKEAIAARGSMLVAFSGGVDSTLLAVFAKEILGNN
                     TSCVLLDSPVVPRAEVEQAIRTASELGLDLDIIPVPLMDQIDFRKNPANRCYICKKIS
                     AAYLKKRAEERGRTSIADGINISDMAEHRPGLAASTEEGIIHPFIESGITKHDIREIS
                     RGRGLLVWQKPSAACLSSRIPYGEEITGKKLRMIEEAEAFLAQRGFGQLRVRVHGTIA
                     RIEVHKEEMAKLLDMEAALAKKLKSIGFSYVTLDLEGYRSGSMDEVL"
     gene            6399..7193
                     /gene="fdhD"
                     /locus_tag="VFC43_02555"
     CDS             6399..7193
                     /gene="fdhD"
                     /locus_tag="VFC43_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HZK30102.1"
                     /translation="MNEKMYRKLPCIKVDGETHEKVLHEVVEEVPIALFVNGRHAMTA
                     MMSPVQLEDFVTGYLFTEQIIKGVDEIESIRIEPNRISVITKNLFKVLGPKKTILSGC
                     GGSTSYIDAEKLPKIKSDFMITIERINTSVRDELSSDLHRTTGGIHVVALLDAEKVIS
                     VSEDIGRHNALDRVIGHGLRTGTDFSRTFIVSSGRISSEMVRKCLIANIPMIISRSAT
                     TTLSVDIAEKTGLTIVGFVRAGKMNIYSQPQRIAGTETVSGLLPEQ"
     gene            complement(7179..>7416)
                     /locus_tag="VFC43_02560"
     CDS             complement(7179..>7416)
                     /locus_tag="VFC43_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00269 family protein"
                     /protein_id="HZK30103.1"
                     /translation="PECPYTHYALRREVRSMLSRFEYKHPGTMIHLLESKEKIERNCK
                     GPVISEALRSCRECGDPCSGELCQVCLLMQSLFR"
BASE COUNT         1751 a         1914 c         1860 g         1891 t
ORIGIN      
        1 gatgggaggt gtcagccagg ccgggggggg atgtctctgc cccgagtata cactccttgc
       61 agcaattttc cgtcatatgc ggctattacc ggatgaagta gttctgctgg atacgccggc
      121 aggaatggag catttcggca gggctgtggc tgatggtttt tcctgtgcgc tggtagttgc
      181 tgacccgtca tataacgctc tcagtgtggc aaaggaatgt tcattacttg cccgtcaact
      241 gggaatcaac gacagtatcc ttgtagtaaa cagggttggc agtcctgcag aagctgataa
      301 aatacacagg accatggatg ggggcagtgg attcttccgg acaattgaat taccctttga
      361 tccggaattt gtttttgctg aacccgctgt cagccaactc ctttccggtg attcagaatt
      421 tatcaaaaaa gccagggttc ttacagaaat tatcactaat tagctggtga ttccctcttg
      481 attttgtgat ttctttttct aaaacccttc attatatttc ttgccacacc aaccatctgg
      541 gtgatgaacc cggtacatcc aggccagacc ccgtccattc caccagctgc tgaaggtttt
      601 ttttcccgtc atccttcgcg ggcagatcac ttcacggaat cagttatccg ggaaatgacc
      661 cggttatccc tgaactacaa tgcgattaac cttgcccagg ggtttcccga tttttcctgt
      721 ccggtagaac tcaagaacgc tgcctgcgat gcggtgcagg aagacatcaa ccagtatgcc
      781 atcacgtggg gggcgcagga ccttcgcgag gcgctttcag cgagagtgaa aaaatataac
      841 ggcatgacct ttcattcgga cacagaaata acggtcacgt gtggttccac cgaagcgatg
      901 atggcgtcca tgctcgcggt tatccagccc ggtgatgaag tggttgtgcc tgaaccattc
      961 tatgaaaatt atggccccga tgcgcagatc tccggggcag ttcctcgttt tgtgccgctc
     1021 cgggatgatt tctctctcga tgaggaggca tggaaatcgg ctttcactca aaaaacaagg
     1081 gcactcatac tcaacagccc caataacccc accggcaagg tattttcgaa aagcgagctt
     1141 atgttcatcg ccgaactctg cagcgattat gatgttgtcg cgattaccga tgagatttat
     1201 gaacatatcc tctacgacaa gaagaaacac atttctctcg gatcaatgga agggatgcag
     1261 gaccggacta tcacgatcgg gagtttttcc aagacctaca gtgttaccgg gtggcgtgtg
     1321 ggatatgcac tcgcagatgc ccgaatcacg gaaaggatcc gaaagatcca cgatttttta
     1381 acagtcgggg ccccggcacc tttacagcat gcctgcgttg ctgcactcag gctgcccgag
     1441 acctattacc aggaactggc agccatgtat gaccgcaaaa gaaagatcct ttttgccgga
     1501 ctcagaaagg ccgggttcag atgtgagctg cccgaagggg cgtattacat ctttacggat
     1561 atttccggat ttgatatgac ggatactgaa ttcgcacggt acctcgttga gaaaattggc
     1621 atagccgcag ttcccggcag ctccttttat cagaacggcg gccagactag gcttcggttt
     1681 acgttttcga agaaggatga gacgctggta gaggcatgcc ggcgactcgg gaacattaat
     1741 caggtctgat cgccttaaaa atgaggaaaa aacgattctt atttttcaaa ctggctactg
     1801 cttcagggtg tataagagat actaacgggg tttcccgctc ccgtgccttt ggtcgcccgg
     1861 cacccaccgc cccccatccc tggtcagctc ttttttccat atcggtacac cagccttgat
     1921 tccctcgatg ataaaccggg atcctgcata tgcttctttt cggtgacctg cggcaacaat
     1981 gataatcaga atattctctc ctactgaaag ccgcccgatg cggtggataa tatcgacatg
     2041 caggagcctg aactgttctg tcgcgtcctt tgcgatcttc tggagttcct gaattgcaac
     2101 atcctcgtag gcttcgagtt ccatctccgt aatatcatca tccctgacaa caccatcgaa
     2161 gacgacaacg gcacccgtcc ccatcttctt tgctgcatca atgagggccc cgatatcgac
     2221 atcccctgtc tggatcgcaa tcatggttca cctgctttat ccacccgcaa ccggcgggaa
     2281 gacggcaatc tcatcaccat cctcaaccgg ggtctgtgca gcatctgcga tttcgatccg
     2341 tttaccgttc ttcatgagga tcacaaactc gcggaatgcc ccggtctcat cgaagatggc
     2401 agcataacct tcgggatttt tctgtgcaat attcgtaatc agcggagtga acatctttcc
     2461 cgcatccaca tcagtgataa tgtcgttccc gagcagttcg cggaaccgtg caaaaaaccg
     2521 gatttttacc gtcatttctt catctccagg atcgtggtct tcgtgaccag gccaccgcag
     2581 gcttcgcagc cgtaattgcg ctccacacga atctcttcgg cacgcgcctg caacccgtcc
     2641 cagatcatga gacgccccgt gagcagttca ccgattccta aaaagtactt cagcacttca
     2701 gttgcctgca ccataccaat aaaccccggt gtcacaccca ccacggggaa cacttctgcc
     2761 ggtggtgctt tggggatgat gcatttcagg cagggtgttt tgccgggaat aatcgtggtt
     2821 acctgcccat agaaaccccg gatcccgcca tggaaaaacg ggagctcttt ttttatggca
     2881 acatcattga gcaggtagcg gatcgggtag ttatccatgg catccacgat accatccgcg
     2941 tttcccacaa ggtttgctgc attcgcagtg tcaattttca catcaatggc attgacggtt
     3001 atgtccggat tgatttcttc caatttttcc tgcgctgatg cagtcttctt cctgccgata
     3061 tcccggtcga aatggaggat ctgacggttc aggtttgtct ggtcaacggt atccatgtcg
     3121 acgatggtta tagtgcccac acctgcgaca gcaaggtaga tggcgaccgg ggacccgagc
     3181 cctccggccc cggcgatgaa aatatgtgcc ttcttaagcc gttcctgccc ttcttctcca
     3241 aacgacagga tctggcggct gtagcgttcc tgttcacgtt gagataaaac cattcttcac
     3301 cttttttcct ggccggccgg ggcgtttccc ttagtgctgg caggtacttt cttcatcatg
     3361 ctcatgggag tggggatttt tctcgaccca gggcacaagt cccttcttca cgcggtaatc
     3421 gttgattgcc ttatggatac cctcctcggc cagtaccgag cagtgcatct tgatcggggg
     3481 cagaccatca agtgcctcgg caaccgcctt gttggataat tcccacgcat cctccagcgt
     3541 ctttccccgg acaagttcgg ttgccatact gcttgatgca attgcggcac cacacccgaa
     3601 tgtcttgaat ttggcatcga tcacgatgtt gttttcgatc ttcagaaaga tcttcatgat
     3661 atcaccgcag accgggttcc cgacttcccc caccccgtca gggttttcaa tctcgccaac
     3721 attacgaggg ttcttgaagt ggtccataac cgtttcactg tacattgcat cctccttctt
     3781 ttccgctctt attgtagagc ggcgacatct cccgcagttt tgtcaccact ttgggaagca
     3841 cgtcaagcac aacatccaca tccgcttcgg aatttgcatc accgagggta agccgcaggg
     3901 atccgtgcga tatttctacc ggaagtccgg tcgcaagcag cacgtgagag ggttcaaggg
     3961 acccggaggt gcaggcactc ccggtcgatg cacagatacc ttcatcatcg agccagagca
     4021 gaatggattc tccttcgata aactcaaaac tgatattaac attgccggcc agcctcttct
     4081 cggggtgccc gttcagccgg gagttgggta ttttatcgag cacggtcctg atgagccggt
     4141 cccgcattgc acggatcttt gcattgtgac caggaatatc tgcagtcgcc atctcgattg
     4201 ctttgccaag gccgacaata cctgcaatat tttctgtacc agcccgccgt ttgcgttcct
     4261 ggcccccgcc atggatcagg ttctcgatcc gcacaccttt tttgatatac agggcaccga
     4321 cgcccttcgg gccataaaat ttgtgagccg agagagacag gagatctata ttctgtgcat
     4381 tcatgtcgag gggaatgttg ccgattacct gtactgcgtc tgtgtggaag taaatcccgt
     4441 gctccctcgc gattgcaccc agctctgcga tcggttcaat cgttccgatc tcattattgg
     4501 catacataat cgagatcagg attgtcttgt cggtaatcgc cttttcgaga tcaacgggat
     4561 cgataagccc gtaccggtcg accggcaggt acgtgacttc aaagccttcc ttttttaaga
     4621 actggcaggt atgaatcacg gcatggtgct cgatctgcgt tgttataata ttgttgccgc
     4681 gcttgcggtt cgcaaaagcc acacctttga tcgcccagtt atctgactcg ctgcctcccg
     4741 aggtgaaata aatttcatcc ggttcggcac ccagtgcctt tgcggtctgc atgcgtgccg
     4801 tatcaattgc tttcttcgag gcacgggcaa ttccgtagag agaagaggga ttgccaaagt
     4861 gcttcgtgaa gtaagggatc atggcatcca ctacctctgg ttttacagac gtggttgccg
     4921 catggtccat gtagattgtg cgctgtcctt ccattggttc ccttgtacct gaatatgtgc
     4981 gttgatctgg ggattattct tttctacgcc gtttataccg gtgaccctgt atgcaggcac
     5041 tgcagcggag tgcgtgtgga tattaaattg tcccggagat tgtggttgtt ttacaaataa
     5101 ataacaattg tgaatacaat atattatgtg agaattgtga taatttaaac ggtgtgatac
     5161 aatggaagcc ccctgtcaga aaatagtctg ggacgttctg ccggccatcc gggcagcaat
     5221 cgctgtagag ctcgtcaggt gcggagtatc ccaggtggag gcggcccgta tgctggagat
     5281 tgcaccatcc gcagtctcgc aatacctgtc aggcaaacgt gggtacagga tcgagtttga
     5341 agaggacgtg aaaaaatcca tagcgcaact ggcacaggac ctcatcgaaa agaaacagat
     5401 caacgtaatc gagcggattt gcgggatctg ccggcagctc cgggatgatg aagggggttg
     5461 tggcaatccg gtttctgaac actgcggcac gtagcgcata tatccttgtg tttcccgttc
     5521 gttttgttaa cccgttacgg tgtcccgatg actcatacaa caaaaatcca gctcctgaaa
     5581 gaagctatcg ctgcgagggg atcgatgctt gttgcatttt ccgggggcgt cgacagtacg
     5641 ctccttgcag tgtttgcaaa ggagatattg ggcaacaata cgtcctgcgt ccttctcgac
     5701 agcccggttg ttccgcgggc tgaggtcgaa caggcaatca ggaccgcgtc cgaactcggg
     5761 ctcgacctgg atatcattcc ggttccgctt atggatcaga ttgatttccg gaaaaacccg
     5821 gccaaccggt gttatatctg caaaaagatc tctgctgcat atctcaaaaa acgagcagaa
     5881 gaacggggtc gtaccagtat tgctgacggg attaacatct cggatatggc agaacaccgc
     5941 ccgggacttg ccgcatctac tgaagagggg atcatccatc cgttcatcga atccggaatc
     6001 accaaacacg atattcggga aatatcccgt gggcggggtc ttttagtctg gcagaaacca
     6061 tcggcagcct gcctttcatc ccggattccc tacggggaag aaattaccgg aaaaaaactt
     6121 cgcatgattg aagaggcaga ggcatttctc gcacaaaggg gatttggaca attgagagta
     6181 cgtgtgcatg gaactattgc gcggatagag gtacataaag aggagatggc gaaattactg
     6241 gatatggagg ccgcactggc aaagaaactc aaatccatcg gcttttccta tgtaaccctc
     6301 gatctcgaag gctaccggag cgggagtatg gatgaagtgt tgtgatatat tctattgtta
     6361 cgattcaata gtattcttac gaaccagggg ctgctgatat gaacgaaaaa atgtaccgga
     6421 aactgccctg tatcaaggta gacggggaga cccatgaaaa agtgcttcac gaggtagttg
     6481 aagaagtccc catcgccctt tttgtcaacg gccggcacgc gatgactgca atgatgagcc
     6541 cggtacagct cgaagatttt gtaaccggct accttttcac tgaacagata atcaagggtg
     6601 tggatgagat agaatccatc aggattgagc caaacaggat cagcgttatc accaaaaacc
     6661 ttttcaaagt gcttggccca aaaaagacaa ttttatcagg atgtggcggg agcacgtcat
     6721 atattgatgc agaaaaactc cccaaaatca agtcggattt tatgataacc atagagagga
     6781 tcaatacctc cgtcagggat gagctctctt cggatcttca ccgcactacg ggcgggattc
     6841 acgtggttgc cttgctcgat gcagaaaaag ttatctcggt ttctgaagat atcggccggc
     6901 ataatgccct ggaccgggtg atcggtcacg gtctccggac cggtactgat ttttcccgga
     6961 cttttatcgt ttcatcgggc aggatctctt ctgaaatggt gcgcaaatgc ctcattgcaa
     7021 atattcccat gatcatttcg cgcagtgcaa caacaacatt atccgtggat attgcagaaa
     7081 agaccgggct tactatcgtc ggttttgtgc gggccggaaa aatgaatatt tactcacaac
     7141 cgcagcggat tgccgggaca gagacggtat ctggtctgtt acctgaacag tgactgcatc
     7201 agcaggcata cctggcagag ttccccgctg cagggatcac cacactcacg gcaggaacgg
     7261 agggcttcag aaataaccgg gcctttgcaa ttgcgctcaa tcttttcttt gctctccaga
     7321 agatggatca tagtgccggg atgcttgtat tcaaaacgcg agagcatcga ccggacctcc
     7381 cgtcgcaatg catagtgtgt atacgggcac tcgggg
//
LOCUS       DASRLV010000061         3201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_100701,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000061 DASRLV010000000
VERSION     DASRLV010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100701"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VFC43_02565"
     CDS             <1..457
                     /locus_tag="VFC43_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30104.1"
                     /translation="GLKILLYLIVFVVIVAVVALLTMSVTVTTVAPGVSMPFTTNYGV
                     SFPEGQTITIGNTHIIVLSNQGLIITDIDGDRQNMVVGEKRVISERRAQITTLGVITI
                     LDTHFQIDLVYKGERDNRAYFDMAIHTSQQVPDILLKQLLPNELDARPI"
     gene            470..1183
                     /locus_tag="VFC43_02570"
     CDS             470..1183
                     /locus_tag="VFC43_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30105.1"
                     /translation="MKFTHFSAAMGILLIVLFCSGCMNTAPQSPSQTTVPSPVPSPVS
                     TTPTMATIVATPAVPAYPGALALGQYATFGSEGRQGKATVYKYEIRPNYNWTAPTFNS
                     PGSQAAASGPKEVQRGYITQKPKAGNTFLFVYVSLVNTGTAAIYAPSASQFVASANGT
                     FYNFTSVQGSDVVIDTVSGTQYDYQIGRGGLVGYIQPGESNKADGYLIYEVPAAISLN
                     DTYVLVNLDYQNRAVWRLG"
     gene            1229..2173
                     /locus_tag="VFC43_02575"
     CDS             1229..2173
                     /locus_tag="VFC43_02575"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HZK30106.1"
                     /translation="MKKITIILFLVACLTVGMIPAASAAGINQGWIRIFCNVDGASVF
                     FDNNYQGVISDGQLLVPVYMAVPVHTFRVEKEGFTTAAGTLTLPDPGATTLAYATIRP
                     IITQTPVPPSMYGSMSVNTSPRGAKIYLDGYYRGTSPFAFDQIEPGSHTIEAILDGYY
                     RYTTMIGIAAGSTADVYFTLQAISLAPNSLNITSDPTHAFVYLDKIFVGKTPLALDTV
                     YTGNHDIELNAPGYNSWSTSVNFPGGGSTRSISATMVPLIPTSPNTPVTVSFTPTTKV
                     TVPQTPTQTSTKAGTNPVLIIGAVGIIGLIAGITSRKI"
     gene            complement(2651..3043)
                     /locus_tag="VFC43_02580"
     CDS             complement(2651..3043)
                     /locus_tag="VFC43_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30107.1"
                     /translation="MKKHEVILARHAGKMIIPAPDTTKGMSVLHELDAMRTSEMPPRS
                     PEVLWELAKERKNINAAGNRMSSEVAWNTITGDFSHVPEDLRKYHFTGFDLTSTPKTT
                     PLLPSAKESETPMLPIARSLPIVHGKER"
BASE COUNT          831 a          796 c          729 g          845 t
ORIGIN      
        1 aggacttaaa attctcctgt acctcattgt ctttgttgta atcgtggcag tcgtagcatt
       61 gttaaccatg agtgtgactg taacaacggt ggctccagga gtttctatgc cattcacaac
      121 caattatggc gtatcattcc cggagggaca gaccattacc atagggaata cgcatatcat
      181 tgtgctctca aaccagggcc tgatcatcac tgatatcgat ggcgaccggc agaatatggt
      241 tgtgggcgag aagagggtta tatcagaacg gcgtgcacag atcacaacac tgggggtaat
      301 taccattctt gatacccatt tccagatcga tctggtgtac aaaggcgagc gggacaaccg
      361 ggcatacttt gacatggcga tccacacatc tcagcaggta ccggatatcc ttctcaagca
      421 gctgcttcca aacgagttag atgcaagacc gatctaaagg tgcgggacca tgaaatttac
      481 ccatttttca gccgccatgg gaatcctcct catagttctc ttttgttccg ggtgtatgaa
      541 tactgccccg cagagtccgt cgcagacaac ggtaccgtca ccggtaccat caccggtatc
      601 aacaacaccc acgatggcaa cgatagtcgc aactcctgca gtacctgcat atccgggagc
      661 actcgccctt ggacagtatg cgaccttcgg cagcgaaggg cgtcagggaa aagcaacggt
      721 ctacaagtat gagatccggc cgaactacaa ctggacagca cctacgttca acagccccgg
      781 gtcacaggca gctgcatccg gaccaaaaga agtccagcgc ggctatatca cgcagaagcc
      841 gaaagcggga aataccttcc tgtttgtcta cgtcagcctg gtcaacaccg gcactgccgc
      901 aatttatgct ccttcggcat cgcagtttgt cgcatctgcc aatggaacat tctacaactt
      961 tacctctgtt cagggttctg acgtagttat tgacacggtt tccgggaccc agtatgacta
     1021 ccagattggg aggggcggtc tggtcgggta tatccagccc ggtgagagca acaaggctga
     1081 cgggtacctc atttacgaag taccggcagc aatctctttg aacgatacct atgtactcgt
     1141 caacctcgat taccagaacc gggctgtatg gagactggga taaaaaggaa tgcaggaacg
     1201 aaattatgca ggggaaagat ggttgtgtat gaaaaaaatt accataattt tattccttgt
     1261 cgcatgtctt acggtcggaa tgattcctgc ggccagtgct gcgggaatta accaggggtg
     1321 gatccggata ttctgtaatg tcgatggtgc ttctgtgttt tttgacaaca actaccaggg
     1381 cgttatttca gacgggcagc tgctggttcc cgtctacatg gctgtacctg tacacacatt
     1441 cagggtcgaa aaagagggat ttacgactgc agccggaaca cttaccctgc cggatccggg
     1501 tgccacaacc cttgcctatg ccacaataag acctataata actcagaccc cagttccccc
     1561 gtcaatgtac ggttcaatgt cagtcaatac atctccccgg ggtgcaaaaa tttatctcga
     1621 tgggtattac cggggaacct cgccgtttgc attcgatcag atagagccgg ggagtcatac
     1681 cattgaagca atcctggacg gctattaccg atatacaacg atgataggga tcgcggcagg
     1741 cagcactgcg gatgtttact ttaccctcca ggcaatatct ttggcaccaa attcactcaa
     1801 tatcacttca gatcccacgc acgcttttgt atacctcgat aaaatctttg tcggtaaaac
     1861 acctcttgct ttggatacgg tatacacggg aaatcatgat atcgaactga atgctcccgg
     1921 ctataactcc tggagtacat cagtcaattt tcccggtggt ggcagcacca gatcgatctc
     1981 tgcaacgatg gttcccctca taccaacctc tccaaacact cctgtaacgg tatcattcac
     2041 cccaactacg aaggtcaccg ttccacaaac ccccacgcag acttcgacaa aagcagggac
     2101 aaaccctgtc ttaataatcg gggctgttgg tatcatcggg ctgattgcag gaattaccag
     2161 caggaaaatt taacgtaaat ttttttttat aaaaaggatg atggcagtca tgcaggagaa
     2221 aaatcagata gccgtgggac aaatctatac ccatcttctt cctttacaaa tcgggaaatt
     2281 tcttgaatcc aaccaattct cacttttttt aaaaaaccac aaccttgagg atgcgtggaa
     2341 ccaatttctc gtggaatcac gggacaggcc tgatttatac ggtaccagtg ttctaaaaaa
     2401 tgcgtttatc ttattcctgc tccatatttt tcaccggtat catgaaaaat ttcctggagt
     2461 gttgcgtgat tttttcaaaa gcatcgcacc atggagttcg cacccgctgc ctgtttttga
     2521 actaaaaaac gacctgcaaa accttggtta ttctgaaaat gaggtaaata tagtattttt
     2581 ttagggtcag attcccgatt ggacctcaca acatgatcct ggcccggaat tgacggtatg
     2641 agatatttgg ttatcgttcc ttgccatgaa cgatgggaag cgaccttgct attggcagca
     2701 ttggcgtttc tgactctttt gcagaaggta ataagggagt ggtttttggg gtacttgtca
     2761 gatcaaaacc tgtgaaatgg tactttctca gatcttcagg tacatgcgaa aagtcaccag
     2821 tgatggtgtt ccaggctact tcactgctca ttcggtttcc tgcagcgttt atgttctttc
     2881 tctcctttgc cagctcccac aacacttccg gggaacgtgg gggcatctca gaggttctca
     2941 tcgcatcaag ctcatgcagg actgacatcc ctttggttgt atcaggcgcc gggatgatca
     3001 ttttcccggc atgccgtgcc agtataacct catgtttttt cataggatca cctttaatgc
     3061 gcacactaca acttcaggca tccccaggat ccttcgtatc ttcatctgtc tgacaattgt
     3121 tcagggtctt ttaccaatta ttctccgtaa agcagatttt gaatcattat ttgtcatatg
     3181 ggcagtattt taggccggac c
//
LOCUS       DASRLV010000062         6783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_827730,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000062 DASRLV010000000
VERSION     DASRLV010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6783
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827730"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1690
                     /gene="uvrA"
                     /locus_tag="VFC43_02585"
     CDS             <1..1690
                     /gene="uvrA"
                     /locus_tag="VFC43_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZK30108.1"
                     /translation="PQTARLYAQTQSDWRKRELEGYMRVSPCPACNGKRLKDKVLAVR
                     IDEKSIIGVTDMPVSGSAAWFRHLKLTDKEREIAHQIIKEIQSRLDFLEKVGLGYLTL
                     SRNAGTLSGGEAQRIRLATQIGSNLMGVLYVLDEPSIGLHQRDNRKLIETLRTLRDLG
                     NTVLVVEHDEDMIRSAEHVIDMGPGAGMHGGHVVAEGNPKQIEKNKKSLTGQYLSGLK
                     MIDVPKTRRAPKRYITIKGCKENNLKNITAKFPIGLLTVVTGVSGSGKSTLVYETLYK
                     GMMQIINKSRDQAGKHEKIVFDAEIDKVIVIDQSPIGKTPRSNPATYTKVFDEIRTVF
                     AGTKEAKLRGFAPGRFSFNIRGGRCEACEGDGLIKIEMNFLPDVYIECEECKGSRYNR
                     ETLDVKYKGKSIAEVLNMSVEEAMVHFEHIPSIRTKLETLSRVGLDYIKLGQSSTTLS
                     GGEAQRIKLTRELAKRATGRTLYLLDEPTTGLHFDDTKKLIKVLDDLVEKGNTVIVIE
                     HNLDVVKSADHLIDIGPEGGDAGGEIIATGTPEKIAATKESYTGQFLKPILQRI"
     gene            complement(1988..2197)
                     /locus_tag="VFC43_02590"
     CDS             complement(1988..2197)
                     /locus_tag="VFC43_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30109.1"
                     /translation="MMRATMISGKLIVINKPFCCIPKSCNSIGEKKLMIPTDIRLKKI
                     ISGIEHKIVRVKFFGSFLYHPPVRQ"
     gene            complement(2570..2842)
                     /locus_tag="VFC43_02595"
     CDS             complement(2570..2842)
                     /locus_tag="VFC43_02595"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZK30110.1"
                     /translation="MGWTYTYAKGIPIEAQYLYKKAMDLYNIGKTESALKYLRQSMVI
                     APRYSKALFDMGNCLTKLGLHDEAAIRFEQAVQIDPALKNILPKRD"
     gene            3129..4709
                     /gene="uvrC"
                     /locus_tag="VFC43_02600"
     CDS             3129..4709
                     /gene="uvrC"
                     /locus_tag="VFC43_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HZK30111.1"
                     /translation="MIDLSTLTDNPGCYLYSDETGTVIYIGKAKNLKKRVTSYFQKTD
                     QDPKTRNLVARIASVSVIVTNTETEALLLENNLIKKHLPKYNIDLKDAKRFAYIELTR
                     EPFPRIGFARRTRAGESTYFGPFVSAAERDAILKVIKRIFRLRSCKKMPKRACLRYHM
                     NSCSAPCIGLISEQDYHTEIERATALLKGKSGELLSALRSEMSAYSTAQDYEKALVLR
                     NQILAIERLAERQHVERPKETDQDIIAYTVADDIVYLMVFSVEKGLLSGKQEYSFDNR
                     EDFFEEFLVQYYADRTPPAELVLPHEVDEALAGYLAQQKGRGVLVTVPKIGEKKKLLE
                     LVEKNIEQAFLKNELKVKELQTALDLPEPPEVIECFDISHLSGTSMVGSMVQFRNGAP
                     DKKNYRRFKIRTIEGIDDPASIAEIVKRRYHRLLEENTDLPDLIIIDGGKGQLSSTLD
                     VLQGLDLLIPVIAIAKKEEEVYMPGEMLPQKLDPKGMALRYIQEIRDEAHRFAIAYHK
                     LLRSKKMTDRHARSGRHG"
     gene            4786..4998
                     /locus_tag="VFC43_02605"
     CDS             4786..4998
                     /locus_tag="VFC43_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30112.1"
                     /translation="MAFFAWVTLPMIVIAGIILFLCLIGTATLGAMIMKGKGNIPFSW
                     HVNLARLVIVIALIHAFFAMAWFLGW"
     gene            5035..5403
                     /locus_tag="VFC43_02610"
     CDS             5035..5403
                     /locus_tag="VFC43_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30113.1"
                     /translation="MKMSVLLIALVISLVFFSGCLSSSVVRENSDGGLRAHYEYSESW
                     SPNQGCYERLTGYVYNVGNNSVDSVRLNFNLVNTGTGTIRDSRSVFIGTVGKGETRTY
                     ETVLDGECTQDYRVEIAFEK"
     gene            complement(5723..>6783)
                     /locus_tag="VFC43_02615"
     CDS             complement(5723..>6783)
                     /locus_tag="VFC43_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZK30114.1"
                     /translation="PQEWSVIERSALFLALILIIIIGLKTIEFIFNLLLISLILTLMT
                     IPAMDWLKKRGVPAGIAVMVVTTLACLCVLALILLTFYSFQVLLADLPMYQTDLNQRL
                     ADITTLLGKYGLDASTASTSSVKLMDIAPTLLSSMISISEALMYVFFIGVTTFFMLLE
                     VPNFTHRIEKIYAAQTEKFHQMSRMSGYIIDFIIVRTETNLVHGVLFGGILSVMGVHA
                     ALLWGILTFFLGYIPYLGLVAAALPAIFFAYLQFGMWGAAAVIFVVVVLNLVVENPVF
                     SYLASRKFEMPALVVILSVIFWGWLLGIAGMLFAVPITLMGLVFIQCYDDLRWLNELL
                     GVSQLFDDGEPDSRDGQE"
BASE COUNT         1784 a         1673 c         1741 g         1585 t
ORIGIN      
        1 cccccagacc gcccggctct atgcccagac ccagtccgac tggcggaaac gcgagctcga
       61 aggctacatg cgggtctccc cctgcccggc ctgcaacggg aagcggctca aagataaggt
      121 gctcgcggtc cggatcgatg agaagtccat cattggtgtc accgacatgc cggtctcggg
      181 cagcgccgca tggttccggc acctgaaact gaccgacaag gagcgggaga tcgcacacca
      241 gatcatcaag gagatccagt cccggctcga ctttttggaa aaggtcggcc tgggatacct
      301 taccctctcc cggaacgccg ggaccctctc cggaggggaa gcgcagcgga tccggctcgc
      361 cacccagata ggttcgaacc tgatgggcgt gctgtacgtc ctcgatgagc cctccatcgg
      421 gctccaccag cgcgacaacc ggaagctgat cgagaccctc cgaacgctcc gtgacctcgg
      481 gaacacggtg ctcgtggtcg agcacgacga ggatatgatc cggtccgcgg agcacgtcat
      541 cgacatgggc cccggtgccg ggatgcacgg cggccacgtt gttgcggaag gaaacccaaa
      601 gcagatcgag aagaataaaa agtccctgac cggccagtac ctctccggct taaagatgat
      661 cgatgtcccg aaaacacgga gagcacctaa acggtacatc acgatcaagg gctgcaaaga
      721 gaacaatctc aagaacatca ccgcaaagtt cccgatcggt ctcctcacgg tcgttaccgg
      781 tgtctcgggc agcgggaagt cgacccttgt gtacgagacc ctctacaagg gcatgatgca
      841 gatcatcaac aagtcccgcg accaggccgg gaagcacgag aagattgtct ttgacgccga
      901 gatcgacaag gtgatcgtga tcgaccagag cccgattggg aagaccccgc gatccaaccc
      961 cgcgacctat acgaaagtat ttgatgagat ccgtacggtc tttgccggga cgaaggaggc
     1021 gaagctgcgg ggctttgcgc cgggccggtt ctcctttaac atacggggcg gccggtgcga
     1081 ggcgtgcgag ggcgacggcc tcatcaagat cgagatgaac ttcctccccg acgtctatat
     1141 cgaatgcgag gagtgcaaag gttcgcggta caaccgcgag accttggatg tgaagtataa
     1201 ggggaagtcg atcgccgaag tcctcaacat gagcgtcgaa gaggcaatgg tgcacttcga
     1261 gcacatcccg tcaatccgta caaagctcga aaccctctca cgggtcggcc tcgattatat
     1321 caagctcggg cagagctcga cgaccctgtc gggcggtgaa gcgcagcgga tcaaactcac
     1381 ccgtgaactc gcgaaacggg ctaccggaag gaccctctac ctcctggacg aacccacgac
     1441 aggactccac tttgatgaca ccaagaagct gatcaaggtg ctcgatgacc tcgtggagaa
     1501 gggcaacacc gtgatcgtga tcgaacacaa cctcgatgtg gtcaagtcag ccgaccacct
     1561 catcgatatc ggtccggaag gcggggatgc cggcggggag ataatcgcga ccgggactcc
     1621 ggagaagata gcggccacaa aggagagcta taccgggcag ttcctcaaac caatacttca
     1681 gcggatataa tggcagaatc aatttttttt aatttctccc ggaaggctgc gatcctgatt
     1741 ttgggtctgc ggaatttttc aaaattttac attgatttca gacgatatca tcctcattct
     1801 cactcgtgat gaatattaaa tatttaaaaa tgatagcctg gaaacggaga accctgcagg
     1861 atgacggaat tcttattatt ccaaaaggcc agtcgcattc atcccatttg tgattcatac
     1921 aaggaggaag attactttga agatacgaaa taaatttcct gaatatgatg aagaaaaaaa
     1981 caatttttta ctggcggacg ggtgggtggt ataaaaagga accaaaaaat ttcaccctga
     2041 caattttatg ttcaatcccg ctgatgatct tcttaagcct gatatcggtt ggtatcatca
     2101 gttttttttc gccaatacta ttacaggatt tcgggatgca gcaaaatggt ttattgatga
     2161 caattaattt tcctgatatc atcgttgccc tcatcattat ttatgcaatt atttatattc
     2221 atgaaatcat ccacctgatt tttatccctg attttttgca ttcggatact acatttctag
     2281 gattatcgtg gtttggaggg tatacctata cgtaggaagt gacaacgaag gcgcgatatg
     2341 tagtaatcgg agctatgctt ttttttataa tctcggtact atttgtcgta attctgggtg
     2401 taacggataa tttaactcct taatgaagtt ggtctgtatc atgaatgcat tagggagctc
     2461 ggttgatttt ttactattct cctggtcttg ctgcaggtac ctgataaaag taaaattgtt
     2521 atgaatggcc cgttatcgta tcataaagtg atgtgatttt tagtatatat cagtcccgct
     2581 tcgggagtat atttttaagt gccgggtcga tctggaccgc ttgttcaaag cgtatcgctg
     2641 cctcatcatg cagtcccagt tttgttaagc aattgcccat atcaaaaagc gcctttgaat
     2701 aacggggggc gatcaccata ctctgcctca ggtatttcaa cgcactctct gttttaccga
     2761 tgttgtacaa atccattgct tttttgtaga ggtattgcgc ctcaatcggt atgccctttg
     2821 cataggtata cgtccatccc ataccacacc tcgatcagcc ggcatgtaac aaaaaaacag
     2881 cgtgtatcta agtattttta taaatattaa aagatatgga tgatcgtggt acattgcagt
     2941 atggaagtgt gtgggatttt cgtgaatggt gatcgggaat tttcggtaca ttgatcgaaa
     3001 caacccggtt tttctctctc aggcaggtct ctgtccggat acctctagtg aaaattctgt
     3061 tcccggtccc aaaaatccat gccccttccc cattccttat gactttgcac tctgacttag
     3121 taccaggaat gatcgatctc agcactctca cggataaccc tggctgttac ctgtattccg
     3181 atgaaacagg tacggttatc tatatcggga aggcaaagaa tttaaaaaag agagtgacga
     3241 gttatttcca gaaaacagac caggatccca agacccggaa cctcgttgcc cgcatcgcat
     3301 cggtcagcgt gatagtgacc aatactgaga ctgaagcgct ccttttggaa aataacctga
     3361 tcaaaaaaca cctgccaaaa tacaacattg acttaaagga cgcaaaacgg tttgcgtata
     3421 tcgaactcac ccgcgaacca tttccccgca tcgggtttgc ccggaggacc cgggccggcg
     3481 aatcaacgta ttttggccca tttgtctccg cagcagaacg cgatgcgatc cttaaagtca
     3541 taaaacggat cttccggctc cgttcctgta aaaaaatgcc aaaacgtgcc tgcctgcggt
     3601 accacatgaa ttcctgcagt gctccctgca tcgggttgat cagcgaacag gactatcata
     3661 cggagatcga gcgggcaact gcccttttaa agggaaaaag cggggaactg ctctctgcgt
     3721 tgcgatcgga gatgtcagca tactcgactg cacaggacta tgaaaaggcg cttgttctcc
     3781 gcaaccagat ccttgcaatc gagcggctgg ccgagcgcca gcatgttgaa cgcccgaagg
     3841 agaccgatca ggacatcata gcctacacgg tggctgatga tatcgtgtac ctgatggttt
     3901 tctcggtcga aaaggggctg ctttcgggca agcaggaata ctcgtttgac aaccgcgagg
     3961 atttctttga ggaatttctc gtgcagtatt acgcggaccg cacgcccccc gcagagctgg
     4021 tactccccca cgaagtcgat gaggcacttg cggggtacct cgcgcaacaa aaagggcggg
     4081 gagtcctggt gaccgtgcca aaaatcggtg agaagaaaaa gctgctcgaa ctggttgaga
     4141 aaaatatcga acaggcattc ttaaaaaacg agctgaaagt caaagagctg cagacagccc
     4201 tcgatcttcc cgaacctcct gaagttatcg agtgtttcga tatctcgcac ctctccggta
     4261 catcaatggt cggttcgatg gtgcagttcc gtaacggggc accggacaag aaaaattacc
     4321 gcaggttcaa aatcaggacg atcgaaggta ttgatgatcc ggcttcgata gcggagattg
     4381 taaaacggcg atatcaccgg ctattggaag aaaatactga cctgccggac ctgattatca
     4441 tcgatggggg aaaaggccag ctctcctcaa cgctggatgt actgcagggc ctcgacttac
     4501 tcatacccgt gatcgcgatt gcgaaaaaag aagaggaagt gtatatgccc ggcgagatgc
     4561 tcccccaaaa actggatccc aaaggcatgg cgctgcggta tatacaggag atccgtgacg
     4621 aagcgcaccg gtttgccatc gcataccata aactactcag gagtaagaaa atgacagatc
     4681 gccatgccag gtcaggccgg catggttaag gtataccaaa ctattccaga ccgggatcaa
     4741 acaatcccgt gaaacattta tcagggatgg atgtgacgac tgtatatggc attctttgca
     4801 tgggtaaccc ttcccatgat cgtcattgca gggattatcc tgttcctgtg ccttattggt
     4861 actgcgacgc ttggcgctat gatcatgaaa gggaaaggta atatcccgtt ttcctggcac
     4921 gtgaacctgg cacggctggt gatcgtgatt gctctcatcc acgctttttt tgcaatggcg
     4981 tggtttttag gatggtagat accaggtatg acgggtattt ggggaggtaa tccaatgaaa
     5041 atgtctgtac tacttatcgc actggtcatc agccttgttt ttttctccgg ttgtctcagc
     5101 agctctgtgg tccgggaaaa ttcagatggt gggctccgtg cccattacga gtattctgaa
     5161 agctggtcgc cgaaccaggg ctgttacgaa cgtctcaccg ggtatgtcta caacgtcgga
     5221 aacaattccg ttgattcggt acggctgaat tttaatctcg tgaacaccgg gaccggtaca
     5281 atccgggact cgaggtcagt ctttatcggt acagtaggca aaggcgagac ccggacctac
     5341 gagacagtgc ttgacggtga atgcacgcag gactatcgtg ttgaaatagc atttgaaaaa
     5401 taatttttcc ggctcgggag aattcctttt atcagagtac aaaaaataag cacagatgcc
     5461 ttgaaggagg ggacagtgcc tgtccccccg ggccgcctct gcgctgtgcg atagtgctgt
     5521 attaccttcc tcgggagtat cgtgtatggg ggaagccgaa gcagcggggg tgaaaccccc
     5581 atgaatgtta aaatttttta ccctttaata atgagatatt ctattatttt ttgcctgcgg
     5641 agagtatccc tcggctgatt acagagttat ccagtccatc ccgtacgatg ccggtatcat
     5701 atccgtaatg aagtccggtt tgttattcct gcccatcccg ggaatccggt tccccatcgt
     5761 caaagagctg gctgaccccg agaagttcgt ttaaccagcg gagatcatcg tagcactgga
     5821 taaaaacaag tcccatcagc gtaatcggga cggcaaacag cataccggcg atccccagca
     5881 accagcccca gaatatgacc gacaggataa cgacaagcgc cggcatctca aatttgcgcg
     5941 aagcaaggta ggaaaaaacc gggttttcca cgaccaggtt gagcacgaca accacaaaga
     6001 tcactgccgc tgccccccac atgccaaact gcaggtaggc aaagaagatg gcaggcagcg
     6061 ctgcggccac cagcccgagg taagggatgt agccgaggaa aaatgtcagg ataccccata
     6121 acagggctgc atggactccc ataactgaca ggattccccc aaataacaca ccgtggacga
     6181 ggttggtctc ggtccttacg atgataaagt caataatata cccgctcatg cgggacatct
     6241 gatggaattt ttcagtctgg gctgcatata tcttttcgat ccggtgggtg aaatttggca
     6301 cttcaaggag catgaagaag gttgttaccc cgatgaaaaa cacatacatc agcgcttcac
     6361 tgatgcttat catcgaagac aggagtgtcg gtgctatatc catcagcttg accgacgatg
     6421 ttgaggcagt gctggcatcg aggccgtatt ttcccagtaa agttgtgata tcggcaagcc
     6481 gctggttcag atcggtctgg tacatcggaa gatcggcgag gaggacctgg aacgaataga
     6541 acgtcaggag aatcagggca aggacacaaa gacaggcaag cgttgtgact accatcactg
     6601 caataccggc aggcacccct ctctttttaa gccagtccat ggcggggatt gtcataaggg
     6661 tcaggatcag cgagatgagc aggagattga agatgaattc gatcgtcttc aacccgatta
     6721 taatgatgag aataagagca aggaacaggg ctgagcgctc tattactgac cattcttggg
     6781 ggg
//
LOCUS       DASRLV010000063         8396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_421847,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000063 DASRLV010000000
VERSION     DASRLV010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 8396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8396
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421847"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            582..2033
                     /locus_tag="VFC43_02620"
     CDS             582..2033
                     /locus_tag="VFC43_02620"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HZK30115.1"
                     /translation="MKRIPGKNLVLTILVLIMTGWCIFPVTALVCDGPPQSQSFILFN
                     GGCDQSRPDLPCVQGFMFPAQPSVGGDFPAQYALDFGDGAPPYYGPVDGVTHTYNRPG
                     IFTLKFMAGTQCDRWRSGSFVLNIPAPLNYSPATPECVPVHPSAGFTGSPTSGAAPLT
                     VQFTSTSRGANAYSWNFGDGGTSPAQNPRHTYSVPGIYSVDLDARDSCSGNTDRAGMN
                     NFVTVTSPVTTLSIASNPSGAAVFIDYIIQGITPLTLTDTATGNHRITISLDGYDEYT
                     RNILLESAGSSTISAELKKSVPQTTTPPPSNGTITITTIPPGASVTVDGIQNGIAPLV
                     IPGVLPGTHGVTLSSDGYEDWNYMVSVRPGQTSEINAVLVAKKESTGSLVVITEPPGA
                     EISVDGFFKGVSPVTITGLAPATHTVLITLGGYTNATTNISITPGQTQKYTLDLQKNY
                     TPSSTDLLLAGGVIVMIAVIAIIVMVKKDPKKK"
     gene            2118..2531
                     /locus_tag="VFC43_02625"
     CDS             2118..2531
                     /locus_tag="VFC43_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZK30116.1"
                     /translation="MALKENILEVVGGPHVAAIATIDGKLPAVRFMVLNGFPDMTFVG
                     ATMKITKKVAQLKKNPNVAISTWSGKEFSDPYVEMMAKGEVHEDLATKKKYWNPMFEQ
                     YFKTVDNPDYVVLVFTAAEIIYHGKNMMTEEVWKR"
     gene            complement(3144..4112)
                     /locus_tag="VFC43_02630"
     CDS             complement(3144..4112)
                     /locus_tag="VFC43_02630"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZK30117.1"
                     /translation="MVDISNLFLQKKPDIHKLAETKNISGLIRALHFSDLQVQSEAAK
                     ALGSLGPPAMDVLIRALKKKGKDHRLVIIKALIEIRDPRAVPALIEILHDENSEVRWE
                     AAIALGEIPDPQSVEHLVTCLRDYDKYVRYGAAFSLEMIGWKPPNDEQRAFLFVGMQE
                     WKAVHLIGNAAIPALSNILHDRDSNIRMKAVEILGELKSHQAIPPLMQSLADKDSEVR
                     WKAVMASPKCGINLIHLPRGLARRPKMKKNPLIAGFLNFVLPGLGYAYLAKWWGVMIF
                     EIDIFGTVWAYQYWGEQFTFETLLPIYLLLAIHAYYITAKMPDDPP"
     gene            complement(4117..5064)
                     /locus_tag="VFC43_02635"
     CDS             complement(4117..5064)
                     /locus_tag="VFC43_02635"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZK30118.1"
                     /translation="MTGIMDIEAMKNKGDIHGLIRLLDHRKNEVQWRAADALGTLGEA
                     ACEPLLRPLAYPRIHVRIGAVEALGAIKSKRSTEHLIHTLMTDTSSEVRWVTALALGE
                     IRDKRAIPALLASLKDKDRYVRYGSAKALEQIGWLAETDLDKAYYYIALEDWNAITKM
                     GKPAIGPLIAMLSDEHPAIRVRIIEILGSTGDAEARNSCKRVLRDPDEHVRWSGVLSS
                     QRCGVPLSHLPLELSHRVRTGKSVTGATILNFFFLGVGYDYLGKWWGLLVLEIYMLLF
                     FIGQLLFGILVTIIIMVPFMTLFAAQTYVMARKEAAEHG"
     gene            5292..5924
                     /locus_tag="VFC43_02640"
     CDS             5292..5924
                     /locus_tag="VFC43_02640"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HZK30119.1"
                     /translation="MDLAMVKFECNGCGRCCVSFGEFIKVERKVAAHDYYCRYGITNE
                     LFHVHVLPEYGNEFAEKFVELEANAKNAPQQGCVFMHRKTGGPGFLCSIYPDRPTICK
                     EFLCYRMLINDSRTGELRGKVIGMNELRTHDAILEAIWKEKITHLPHPFESEHHKVKH
                     SHMPGAENVHGHDPHILAHIYGLEHTDDREWVENVLTVLASHGYQGDPVD"
     gene            complement(6965..7522)
                     /locus_tag="VFC43_02645"
     CDS             complement(6965..7522)
                     /locus_tag="VFC43_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30120.1"
                     /translation="MAVRHLAFLTIVLIFLTLVAGCTDSSTESPPLQTMSTLPVPGSG
                     SQDTPLLQVFGDTTGKGIPGGTIDTITFAVGLIDIHKSVDMEHLAIVYADAIRTETLR
                     PVEGFHGDPPKGFWSITRVENEVGDPNNRLDNEERFFITINPKAPLVPRQILTIDVKP
                     QNGHSLTIRRVAPPSIFEDNILLPP"
     gene            7671..>8396
                     /locus_tag="VFC43_02650"
     CDS             7671..>8396
                     /locus_tag="VFC43_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30121.1"
                     /translation="MTLHFNENDYHLFYHYCANYCRLYDETRKNSIKILLELYKHEVI
                     DIQEHSLDNIFSFFYQKKQEFETEGRVPASDSDNLMRIRICSDIIAFLDDLEKIYRLL
                     RKKNREANYVLIIGVFSKIFEDEAQTRAEDLQKTASDAIDAIVLPLEKEIEKKLGTQA
                     DREAILYEMVSFTEIFNRLSPDLIFNDEVILLIAPTLLKRFGNEENHEEIRNLVKSHR
                     DQFGMDELESALEGGIHARNEWSL"
BASE COUNT         2260 a         1983 c         1970 g         2183 t
ORIGIN      
        1 tcgatgctat acgacaattt attgaacata tagaatcaac ccataaaatt cctgtattaa
       61 cctccgacgt tatttttcga ttaaagaatg gcataaaaaa ataatttccc tgatcatgca
      121 tatttgcatt ctataatcat ggtgtgggca ggcagtcata atccctgtca ggtaaaagcg
      181 ggtatgatta tttttgataa tcagaaatgt gatggaagtg ttttttactg aaaaaatctc
      241 ttctgcggaa tatactgact tttttcatat tcatgaatat gctgaccttt gcttttgtgt
      301 acagattttt tacctgaaac agtgcataac cacaaaggtg ctcattttca cctcacccgt
      361 ggcgctgaaa ccataatgta tctggcggag tgcaggtgcg gggaacagca aaagtccgaa
      421 caacggtctg cgaacggtaa actttgtcga taccttcata aaatagtata caatcagggc
      481 acaaggacag ctggtgtttt tacattgaag cgatcaatgt ctttttagca atcggggtac
      541 aaactgcggg agcagtgctg gcgctcaaaa aggagaatga gatgaaacgg atacctggca
      601 aaaatctggt cctgacaata ctcgtactca tcatgaccgg gtggtgcatt ttcccggtga
      661 ctgccctcgt atgtgacggg cctccgcaaa gccagtcatt tatcctgttt aatggcgggt
      721 gtgaccagtc ccgccctgac ctgccctgtg tccagggatt catgttcccg gcccagccct
      781 cagtcggggg agattttcct gcccagtatg ccctggattt tggtgatggc gcacctccct
      841 attacggtcc tgttgacggc gtaacccaca cgtacaacag gccaggtatt tttacgctga
      901 aattcatggc aggcacgcaa tgcgaccggt ggaggtctgg tagttttgta ttaaatatcc
      961 cagccccgct gaattattca ccggctacac ctgaatgtgt cccggtacat ccttcagcag
     1021 gatttaccgg cagcccgaca tccggtgcag ccccgctcac ggtccagttc accagcacgt
     1081 cccgtggggc gaatgcctat tcgtggaatt ttggcgatgg cgggacatca cccgcacaga
     1141 atccccggca tacctatagc gtgccgggaa tctattccgt tgaccttgat gcccgggatt
     1201 cctgttcagg aaatactgat cgggcgggca tgaataattt tgtcacggtt acatcgccgg
     1261 taacaaccct ttcaatagcc tcaaatcctt ccggtgctgc cgtatttatc gattacatca
     1321 ttcaaggaat aacccccctt acacttacgg atacagcgac cgggaatcac cggataacga
     1381 tttctctcga cggatatgat gaatacaccc ggaatatcct gctggaatca gcagggtcat
     1441 caacgatttc cgcggaactg aaaaagagcg tcccgcaaac aacaaccccg cccccatcaa
     1501 acgggactat tactatcacc accatccccc cgggtgcttc ggtgaccgtt gatggcatac
     1561 agaacggtat tgctcctttg gttatccccg gtgtgctgcc aggaacccat ggcgtaaccc
     1621 tgtcatcgga tggctatgaa gactggaact atatggtatc tgtaagaccg ggacaaacgt
     1681 ctgagatcaa tgccgtcctt gttgcaaaaa aagagagcac cggttcgctc gtggtgatca
     1741 ccgaaccccc gggtgccgaa atttctgttg atgggttttt caaaggagtg agcccggtaa
     1801 ccatcacagg ccttgccccc gcgacccaca ctgttttaat cactcttggg ggatacacca
     1861 atgcgacaac gaacatttcc attacacccg ggcagacgca gaagtatact ctggatttgc
     1921 agaaaaacta cactccctct tcaacggatc tgctgctggc tggtggggtg attgtgatga
     1981 ttgcggtaat agcgataata gtcatggtga aaaaagaccc aaaaaagaaa taaatcatat
     2041 ccctttttag ttgcccgttc tccatggttt caggcttccc gtaaaattta atactacaac
     2101 ccgcgagagt gttcaccatg gcactcaaag aaaatatcct tgaagttgtc ggtggaccgc
     2161 acgttgcggc tattgcgaca attgatggca agcttcccgc agtacgattc atggtactta
     2221 acgggttccc tgacatgaca tttgtcgggg caaccatgaa gatcaccaaa aaagtggcac
     2281 aactgaaaaa gaacccgaat gtcgccattt ccacctggtc aggtaaagaa ttttccgatc
     2341 catacgtaga gatgatggct aaaggagaag tccatgaaga ccttgcgaca aagaagaagt
     2401 actggaaccc gatgtttgag cagtatttca aaacagtgga taaccctgat tatgttgttt
     2461 tggtctttac tgccgcagag attatctatc atgggaaaaa catgatgacc gaggaagtct
     2521 ggaagagata attccccttc ttttcttctt tttcattaaa ggcatacctg tggcataggt
     2581 aaaatttttc acctgaaatt atccaggttg acgtatgaaa accagaacga tacgacagac
     2641 tctttccttt catgcacgtg ctcacgaggt ttttgagatg ctgatggatg aaaaaaacat
     2701 gcacttttta cggggggttc tgcacagatc gaccggaacg taggaggaac gttttcaacc
     2761 aacgacgggt attcaacggg cacaaatctc gagctggttc aggacgcaaa aattgtgcag
     2821 acctagcggg caagtgactg gcccgagggt catttttcaa cactcactat tatcctttcc
     2881 ccggtatctt ccaaaacgtt actatccttt gtccagaccg gagttccgtc tgaccagttt
     2941 gaagagatca gcaaggggtg gtacgactat tactggaatc ccttaaaggc attgctggaa
     3001 aaggttcagt gatgggttga atagctcatc gaccgtcttt tgtaaggatt caggtccctc
     3061 aatcctctac tgtgtttaag gggcaaaaag ggaaaatcac catccctgaa cgtcgttctc
     3121 cccctttccc ttaaagaatc tgattatggc ggatcatcag gcatttttgc ggtaatataa
     3181 taggcatgga ttgccagcag cagataaatg ggcaggagtg tttcaaacgt aaattgctct
     3241 ccccagtact ggtacgccca gactgtcccg aagatatcga tctcgaagat catcacaccc
     3301 caccattttg ccaggtacgc atatccgagc cccggcagca cgaaatttaa aaatcccgca
     3361 atcaggggat ttttcttcat tttcgggcgg cgggccaggc cccggggaag gtgtatcaga
     3421 tttatgccgc attttggcga cgccatcacc gctttccatc gcacttcact gtctttatct
     3481 gccagggatt gcatcagcgg gggaattgcc tgatggcttt taagttcacc gagaatttca
     3541 acagccttca tacggatatt gctgtcccgg tcatgaagga tgtttgagag cgccgggatg
     3601 gcagcattcc cgatcagatg gacagctttc cattcctgca tacccacaaa aaggaatgcc
     3661 ctttgctcat cattcggtgg tttccagccg atcatctcaa gagaaaaagc ggcaccgtac
     3721 ctgacatatt tgtcataatc gcgaaggcag gtaacgaggt gttcaacaga ttggggatcg
     3781 ggtatttcgc ctaaggcaat tgcggcttcc cagcgcactt cgctgttttc atcatgtaat
     3841 atttcaataa gtgcgggtac tgcccgggga tcccggatct cgatgagcgc tttgattatg
     3901 actaggcggt gatctttgcc tttctttttt aacgcccgga tcagcacatc cattgccgga
     3961 ggtccgaggc tgccaagtgc ttttgcagct tcggactgca cctgcaggtc actgaaatgc
     4021 agtgcacgga taagaccaga aatatttttt gtttcagcta atttatggat atccggtttt
     4081 ttttgtaaaa agaggtttga aatgtccacc atacgatcac ccgtgctctg ctgcctcctt
     4141 cctggccata acgtacgtct gcgctgcgaa taatgtcatg aaaggaacca tgatgatgat
     4201 cgtcaccagg attccaaaaa ggagctgtcc tatgaagaac aggagcatgt aaatttcaag
     4261 gactaaaaga ccccaccatt tcccgagata atcataccct acacccaaaa agaaaaagtt
     4321 caggattgtt gcgccggtta cactttttcc tgtcctcacc ctatgtgaaa gctcaagcgg
     4381 aagatggctt aaaggaaccc cgcacctctg ggatgaaaga accccgctcc accggacatg
     4441 ctcatcgggg tcccgtaaga cccgtttaca ggaatttctc gcttcagcgt cacctgtaga
     4501 accaaggatt tcaataatcc ggacccggat ggcagggtgc tcatcgctga gcatagcgat
     4561 caggggtcct atcgctggtt ttcccatttt tgttatggca ttccagtcct cgagggcaat
     4621 ataataatat gccttatcca gatcagtttc ggccaaccac ccgatttgtt caagtgcttt
     4681 tgccgacccg taccggacat accgatcttt gtcttttaag gatgccagca atgccggtat
     4741 tgccctttta tccctaatct cacccagagc cagtgcggta acccagcgca cttcgctgct
     4801 ggtgtcggtc atgagtgtat gaataaggtg ttctgtcgat cgcttacttt taatcgctcc
     4861 caatgcctca actgcaccaa tgcggacatg tatcctggga tacgcgagtg gacggaggag
     4921 tggttcgcag gcagcctctc ccaaggttcc cagggcatca gccgctcgcc actggacctc
     4981 atttttccga tgatcaagaa gcctgatgag accgtgaatg tctcccttat ttttcatcgc
     5041 ttcgatatcc atgataccag tcaccgcaaa acaaatttag ctaatactac aattatctcc
     5101 aggattctat atatctgaat caattcgtgt tgtttatgaa attatggatc atataggatc
     5161 aaaaagagta attttcaaaa ataatgtaaa atccacggca tggagtagga aagaaaaaca
     5221 gatttgtcaa aaatccggct gaaaatttcc tgacattata ccccaatttt tataattagg
     5281 gttaacataa aatggatctt gctatggtga agtttgagtg caatggttgc gggagatgct
     5341 gtgttagttt tggggaattc attaaagttg aacgaaaagt tgctgcacat gattattatt
     5401 gccggtatgg aatcacgaac gaactcttcc atgttcatgt gctgcctgaa tatggcaatg
     5461 agtttgcaga aaaatttgtg gaactggaag ccaacgctaa gaatgccccc cagcagggat
     5521 gcgtatttat gcaccggaag accgggggtc cgggttttct ctgtagtatt tatcctgacc
     5581 gccccaccat atgcaaagag tttctatgtt accggatgct gatcaatgat agcagaaccg
     5641 gcgagctccg cggtaaagtt atcggtatga atgaactcag gactcatgat gcgatactcg
     5701 aagctatctg gaaggagaag attactcacc tgcctcaccc gtttgaatca gaacaccata
     5761 aggtgaagca ttcccatatg cctggtgcag agaacgtcca cggccatgac cctcacatcc
     5821 ttgcgcatat ctatggactg gaacatacag atgaccgtga atgggtggaa aatgtcctga
     5881 cagtgcttgc ctcacacggc taccaagggg atccggtgga ctgaaccgtt accttacacc
     5941 ggtttttcgc tataccgtgt aaataccccc gaccggctcc ccatcattct tacctttaga
     6001 ctcctcgatt aatcggaaca tccaaatctt taaacgaggt ctttctttac atcattcttg
     6061 agcatataaa accggctctt gttttttctt tttgctctgt agaaacgcag atgaaccaaa
     6121 agcgttcaca tccaactcat gaaaataggt tttctctacc gatggacccc caattcaggg
     6181 cctctccggg gcacggctgg cagggagttt ttttgaattt tagtcacaga tcctccgcgg
     6241 gggtgccccg tagggggcgt cgttgttcat cgttactgaa gaattgggat gataatcccc
     6301 tgacatacct gaatttccga ttttcatggg aaattatttt tcaaaaattc tgacgctctt
     6361 tttgggcttt tgtccccacg tgggcatcct cttcgcgatg aagatgtatt ccctgttacc
     6421 aaaaaacctg ggtgataaca cagcgccccg tccccaacgg aggcctcatt ggcatgaaga
     6481 ggaggcaata ttttaccaga aaaaagtttt ttacagagta tttcaactgc attccgtaca
     6541 tccagcctga cccggttatg gcattgcggg cactgtactg taaccggttt tttgtgcccc
     6601 ttctgcaggc tggcaagtcg tgaaccaaag tagacccacc cgtggttgca gcgatgacac
     6661 atgacaaaac agcttgagag ttccatgtcc gatcccatgg aattatggaa atatgaacac
     6721 gacccgggcg aagagcgaaa gtgtcggggt tgctttcagg atccgctaac acctccaatg
     6781 cttggtgaca ggagctgaat aaagaggaat actccctata aaaaccgccc ggaatgccgg
     6841 agctggttga aaattcagga aataaacggg actttggcag caggattaaa aatactgctc
     6901 gcatttcaaa aatttcccaa aaaccgcaca attttagtcc agcgccggat tgtactatca
     6961 gggattacgg gggtaaaagg atattatctt cgaaaatgga gggtggagcg acccggcgga
     7021 tggtgaggga atgaccgttc tggggtttta catcgatagt gagaatctgc cgggggacaa
     7081 ggggcgcctt tgggttgatg gtgataaaga accgttcttc attatcgagg cggttgttgg
     7141 gatcgcccac ttcattttcc acgcgggtga tactccagaa ccccttcggt gggtcgccat
     7201 ggaatccctc gaccgggcga agggtttcgg tccggatggc atctgcatag acgatggcaa
     7261 gatgttccat atccacggat ttgtggatgt cgatcaggcc taccgcgaac gtgattgtgt
     7321 cgatggtacc cccgggtata ccctttccgg tcgtatcacc gaacacctgc aggagaggtg
     7381 tatcctggga accggagccc gggactggca gggtactcat tgtctggagt ggcgggctct
     7441 ctgttgagga atcggtgcag cccgctacaa gggtgaggaa aataagcaca atcgtcagga
     7501 aagccaaatg ccgcactgcc atagagagac ccctctgtga tcctgtggta gaaaccatgg
     7561 gtctgtggac tagaaaaaca attgggtagc agcatacaga ataaatattt gttatttacg
     7621 aatgagtaaa ataaaataag aaggactatt gattagtaca catgggaaga gtgacgttgc
     7681 atttcaatga aaatgactac catctcttct atcactactg cgccaattat tgtcgtctct
     7741 atgatgaaac aagaaaaaat tcaataaaaa ttcttcttga actttataaa cacgaagtca
     7801 ttgatattca ggaacattca ctcgacaata ttttttcttt tttttaccag aaaaaacagg
     7861 agtttgagac cgaaggacgc gtacctgcca gtgattcaga caatctgatg cggatccgga
     7921 tatgttctga catcatcgcc tttttagatg accttgaaaa aatatatcgt ctgttaagga
     7981 aaaaaaacag ggaagcaaat tatgtcctga tcatcggtgt attttcaaag atttttgaag
     8041 atgaagccca gacccgggca gaagatcttc aaaaaaccgc gtcggatgcc atagatgcca
     8101 tcgttttacc cctggaaaaa gaaatagaaa aaaaactcgg cacacaggct gacagagagg
     8161 caatcctcta tgaaatggta agttttacag aaatttttaa caggctttca ccggacctta
     8221 tcttcaatga tgaagtcatc ctgctcatcg cacccacgtt gctcaaaagg tttgggaacg
     8281 aggaaaacca cgaagaaatc cggaatttgg taaaaagcca tcgtgatcaa tttgggatgg
     8341 atgaactcga gtcggcactg gagggaggta tccatgcacg aaacgagtgg agtctt
//
LOCUS       DASRLV010000064         6249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_992349,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000064 DASRLV010000000
VERSION     DASRLV010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 6249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6249
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992349"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..312)
                     /locus_tag="VFC43_02655"
     CDS             complement(70..312)
                     /locus_tag="VFC43_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2109 domain-containing protein"
                     /protein_id="HZK30122.1"
                     /translation="MIAVYVCAGIAVYAAIRVIAERNTMRKLPFLNVVSFAVTGIIAL
                     LLPHPITIAAAAAYFVGSTLESNAIASTWAGGIRKP"
     gene            complement(309..821)
                     /locus_tag="VFC43_02660"
     CDS             complement(309..821)
                     /locus_tag="VFC43_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30123.1"
                     /translation="MLDEPLRILIAAIICWLNFMTVDLIFKLPHSGGVSGATAIAQEV
                     ENGGGNLNGGYMMGNIVSSPDASAGTLLASFGVYCAGLPGGLFAALLVYIGNRICYDP
                     GYAGTTGCISATFIVYGAVQSGWFSASDFIAGMVIAILTIQGISYVHSSRLVARLWKV
                     RCRFIPEDKP"
     gene            complement(1082..1957)
                     /locus_tag="VFC43_02665"
     CDS             complement(1082..1957)
                     /locus_tag="VFC43_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZK30124.1"
                     /translation="MFEKVLVTLDFSPHSQKILDRVGEIPGIQEVSLLHVVDATRPSR
                     LGWTHGPHIENTKILMAEKKEVLEKLGLRVHINVDVIVNAITQGTIANAILEIAETEN
                     VSLIVIGARGINPIQELLLGSVSSTVLRDAKTSVLLVRDTAPVSSTAADRPLFSNVLV
                     PTDFSTPAEDALSVIKTLEGIKKVTFLHVVSRAESKTEIDAYVTDAQARLTALQQDLA
                     SAGITSNTHIRVGDPTEMILSVAEEEGVSLIAMNAHGTHWLRTMILGSTTFTVVRRTK
                     RPALIIRTGKEKETS"
     gene            complement(2280..2678)
                     /locus_tag="VFC43_02670"
     CDS             complement(2280..2678)
                     /locus_tag="VFC43_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZK30125.1"
                     /translation="MAKKKTAITMRKCCDFFQDLPDPIRDELDKKGGIEGLLERIPDS
                     SALHASGRLHQALADPIRLMVMHALLSQSLCVCVIKGVVRIADSKLSYHLNILKEQDL
                     ISGEQQGNWIIYSITDRGRKALKCLECMGR"
     gene            2767..3147
                     /locus_tag="VFC43_02675"
     CDS             2767..3147
                     /locus_tag="VFC43_02675"
                     /inference="COORDINATES: protein motif:HMM:NF020434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HZK30126.1"
                     /translation="MEQQQSPCSCGADGNTRIIYACSGIGSNVGQLANAAACRLAHEG
                     FGSGSCLAGVGGGIEKLIGIGKTADERIVIDGCPVACAKKIMDDRDLSIDRYIMITGL
                     GIEKTPGPAYNENDVQAVIDAVHL"
     gene            3144..3488
                     /locus_tag="VFC43_02680"
     CDS             3144..3488
                     /locus_tag="VFC43_02680"
                     /inference="COORDINATES: protein motif:HMM:NF020434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HZK30127.1"
                     /translation="MKTLITCSGLSNTGRLTTQVATILMSRHPAAITWVKAHKGGQPI
                     ADAAEDADLVIVLEGCPDHCASKKTIDAGINADIHLVATELGVEKNGMAEVQWNDVEK
                     VLTAIEQKINGV"
     gene            3493..4452
                     /locus_tag="VFC43_02685"
     CDS             3493..4452
                     /locus_tag="VFC43_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HZK30128.1"
                     /translation="MQDYLQVFADWVTYTIFGLVQGSPLASSVNFFIYDSIKVTLMLA
                     VIVFGVAIIRSFITPQKVKKWVAGRTEGVGNVLASLLGIPTPFCSCSAVPLFIGFVES
                     GVPLGITFSFLIASPLINEVAAAMLLAMFGWQIALIYIISGLFIAIIAGIIIGRLHLE
                     SEVEEFVWKCKLYDQPDKAMTWRDRIDFGISESKAITVKVLPYILAGIGIGAVIHGYA
                     PADFLVNIAGKDNPLAVPIAVLIGIPLYSNAAGMIPIMEVLTAKGMAMGTGLAFMMAV
                     IGLSLPEMIILRKVIKIKLLVIFAGILFVSFTLTGYLFNAILG"
     gene            4478..4912
                     /locus_tag="VFC43_02690"
     CDS             4478..4912
                     /locus_tag="VFC43_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HZK30129.1"
                     /translation="MTKSDEALASFRNGFTCSSAVFSAFSDELGLDSDTAKKIACGFG
                     AGISKTGNICGAVSGAVMVIGLKYGKTKMGDDAATEKTRALVRKFMQEFTKRHGSVCC
                     TELLGYNLGKPDEYEKARDSKLFVTKCPQLVEDAAAILEKFL"
     gene            4935..6011
                     /gene="arsB"
                     /locus_tag="VFC43_02695"
     CDS             4935..6011
                     /gene="arsB"
                     /locus_tag="VFC43_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HZK30130.1"
                     /translation="MEKPAKQLSFLDRYLTLWIFLAMAAGIGIGYFAPQVPAMITGLS
                     VGTTSIPIALGLILMMYPPLAKVKYEELIKVFQNTRVLGLSLVQNWIVGPVLMFALAV
                     IFLRDQPLFMYGLILVGIARCIAMVIVWNDLAEGDCEYCAAIVGLNSVFQVFFYSVYA
                     WFFITYLPPLLGIGKGSAVSITILQIAGSVFVYLGIPFIAGMVTRYALLRVKSKAWYE
                     QVFIPKISPITLIALLFTIIVMFSLKGEYIVNVPFDVVRVAIPLLFYFIIMFFASFWM
                     SYRLGIDYRKSASLSFTAASNNFELAIAVAIAVFGIASPAAFATVIGPLVEVPVLIGL
                     VNVSLWIRDRWYGGKRDNSCSPVR"
BASE COUNT         1561 a         1522 c         1567 g         1599 t
ORIGIN      
        1 cgcgattgat aattgccgca gtcatccctg ccagaatgag aatagctgac gcaatcatca
       61 cgatatcgtt catggtttcc tgatgccccc tgcccaggta ctggcaattg cattcgattc
      121 aagggtggat ccgacaaaat acgcagcggc agcggcgata gtgattgggt gaggtaacag
      181 gagtgcgata ataccggtta ctgcgaatga aacaacattg agaaatggca gcttccgcat
      241 cgtattgcgt tccgcgatga ccctgatcgc tgcatatact gcaattccgg cacagacata
      301 gacagcaatc atggcttatc ctccggaata aacctgcacc gtactttcca gaggcgggca
      361 actaacctgc ttgaatgcac atatgaaatc ccctggatgg tcaggatggc tatcaccata
      421 ccggcgatga aatcagaggc tgaaaaccac ccggactgaa ctgccccgta gacaatgaac
      481 gtggcagaga tacatcccgt tgttccggca tagccgggat cgtaacagat acgattgccg
      541 atatagacaa gcagtgcagc aaaaaggccg ccgggcagac cagcacaata tacaccgaag
      601 cttgcaagaa gggttcctgc ggatgcatcc ggggaggaga cgatattgcc catcatatat
      661 cccccgttca ggttgccccc accgttctca acttcctgtg ctatcgcggt agcaccggat
      721 actcccccgc tgtgtggtaa tttgaaaata agatcaacgg tcatgaagtt gagccagcag
      781 atgatggcgg caataagaat ccgcaggggt tcatcaagca tgggatgcac cggataatca
      841 aatctgatta acactgtaga tcagagggat aacggataaa ggttctgata ttgacgaata
      901 tagtaacaat tacgtgaaga tatgtgttca atcgatatgt ttcgcaggaa cccccgatac
      961 ttcaggcacg ctaaatagta attttcgggt tagatagata taaggacatt atttttcttt
     1021 ggtaatggct ggaggggaga cctgcggagg gtcgcacaaa acgggtatcc gatcgcgact
     1081 attagctggt ttccttttcc ttcccggtcc gaatgatgag agccggtctc tttgttctcc
     1141 gcacaacggt gaatgtggtg cttccgagga tcatcgtgcg caaccagtgc gtaccatgag
     1201 cattcatcgc gatcagggat acaccctcct cttctgcaac ggaaaggatc atctctgttg
     1261 gatcaccgac tcgaatatgc gtgtttgagg ttataccggc agaggcaagg tcctgttgca
     1321 gtgccgtaag acgtgcctgg gcatctgtaa cgtatgcatc gatctctgtc ttggattccg
     1381 cacgactcac tacatgcagg aatgtgactt tttttattcc ctcaagggtc ttaatgactg
     1441 atagtgcatc ttcggctgga gtggaaaaat ccgtaggaac aagcacgttg gaaaacaatg
     1501 gacggtctgc tgctgtcgag gatacgggtg cggtatcgcg gacaagcaga acactcgtct
     1561 ttgcatcgcg gagcacggtt gaggatacac ttcccaacag gagttcctgg atcgggttga
     1621 taccccgggc acctatcaca atcagggata cattttctgt ttctgcaatt tcaaggatcg
     1681 cattcgcaat agtgccctgg gtaattgcat tgacgatcac atcaacattg atgtgcaccc
     1741 tcaatccgag cttctcaagc acctctttct tctctgccat gagtattttc gtgttctcga
     1801 tatgtggccc atgtgtccat cccagtcttg aggggcgggt cgcatctacc acatgcagga
     1861 gagacacttc ttggatccct ggaatctcgc caacgcgatc aaggattttt tgagaatggg
     1921 gtgaaaaatc cagtgtaacc aacacttttt caaacatgct gcctccttac cagattctgg
     1981 tatcctagaa tacatacggt gttttaataa aatgattggt ggatattatg agaacccgct
     2041 ctcgtgatag taaaatcaca aatcagttta tggcatccag aaaagcagga aaaaaggaag
     2101 aggtgtcggt ctctcgtcat gcccggcaga ctggtccgat taagtaggtg ataattgatt
     2161 tcacccagta aatccccgat tcacaattgg gggaagagcg gctcaataat tccaaaatga
     2221 ggaccgcatc agtaacattt gggcagatgc cggcacgtcg gacaacaata aataatcgtt
     2281 tacctgccca tgcattccaa acatttcagg gctttccgtc cgcgatcggt gatgctgtag
     2341 atgatccagt tgccctgctg ttcgccggaa atcagatcct gttccttgag aatgttgagg
     2401 tggtacgaaa gtttggagtc ggcaattctg actactccct tgatgacgca gacgcagagg
     2461 gactgggaga ggagggcatg catcaccatc agccggattg gatccgcgag ggcctggtgg
     2521 agacgtccac tggcatgaag tgcggaagaa tccggtatcc tttccagaag accttctatc
     2581 cctccctttt tatcaagttc atccctgatc gggtcaggaa gatcctggaa aaaatcgcag
     2641 cattttctca tcgtgattgc ggtttttttc tttgccatgg atgattcaaa ttaatatgaa
     2701 atagttaggt atataacctt gtgctgccca caatgattca ataaaatttg aaatgatgtg
     2761 aaagcaatgg aacagcagca atcaccgtgc agttgcggag ctgacgggaa tacacggatc
     2821 atctatgcct gctccggaat cggatcgaat gtcgggcaac tcgccaatgc agcagcgtgc
     2881 cggctggccc atgaaggctt cggcagtgga tcatgccttg ccggcgttgg cgggggcatc
     2941 gaaaaactga tcggcatcgg taaaaccgcc gatgaacgga tcgttattga cgggtgtccg
     3001 gttgcctgtg caaaaaaaat tatggacgac agggatctca gcatcgaccg gtatatcatg
     3061 atcaccgggc tcggaattga aaagacgccc gggcctgcct acaacgaaaa tgatgtccag
     3121 gccgttatcg atgcggttca cctatgaaaa cactgattac atgctccggt ctctccaata
     3181 ccgggcgact gacaacacag gtagcgacaa tactcatgtc ccgccatccg gctgctatca
     3241 cgtgggtaaa agcacacaaa ggcgggcagc ccatcgctga tgcagcagag gatgctgacc
     3301 ttgttattgt tctggaaggc tgtcctgacc actgtgcttc aaaaaaaacc attgacgcgg
     3361 gaatcaacgc tgatattcac ctggtggcta cagaactcgg ggttgagaaa aacggaatgg
     3421 ccgaagttca gtggaacgat gttgaaaaag tgcttacagc cattgaacag aaaataaacg
     3481 gagtataaaa ccatgcagga ttatttgcaa gtattcgccg actgggttac ctacacgatc
     3541 ttcggtctgg tgcagggtag tccccttgca tcctcagtga atttttttat ctacgactcc
     3601 atcaaggtca ccctgatgct cgctgttatc gtgtttggcg ttgctatcat tcgttccttt
     3661 atcactccgc agaaagtgaa gaaatgggtg gccggccgta cagaaggtgt gggtaacgtt
     3721 ctcgcatcgc ttcttggtat ccctactcct ttctgttcct gctccgcagt cccgctgttt
     3781 atcgggtttg ttgagagtgg ggtgcccctc ggaatcacct tctcgttcct catagcatca
     3841 cccctcatca atgaggtagc agcggcaatg cttcttgcca tgttcggctg gcagatagca
     3901 ttgatctata tcatttccgg ccttttcatc gctattattg ccggtattat catcggacgc
     3961 ctccacctag agagcgaggt ggaggagttt gtctggaaat gcaaactgta cgaccagccg
     4021 gataaagcaa tgacatggcg ggacaggata gacttcggta tcagtgagtc caaggctatt
     4081 accgtaaaag tgcttcccta catcctggca gggatcggga taggtgcggt tatccacggt
     4141 tatgctcctg ccgatttcct tgtcaatatc gccggcaaag acaatccgct cgctgtaccg
     4201 attgcagtac taattggcat tccgctgtat tcgaacgctg caggcatgat cccgatcatg
     4261 gaagtgctca cggcaaaagg gatggcgatg ggcaccgggc tcgcattcat gatggcagta
     4321 atcgggctct cacttccgga gatgatcatc ctacgtaagg tgattaaaat caaactgctg
     4381 gtaatctttg cagggatcct gttcgtgtcg ttcacgctga caggatacct gtttaatgcg
     4441 attctgggat agtatactac ccgacaggag aaacaccatg acaaaatctg atgaggcgct
     4501 ggccagtttc aggaacgggt ttacctgttc ttcggctgtc ttttccgcat tctctgatga
     4561 actcggactg gatagtgata ccgcaaagaa gatcgcctgt ggttttgggg cgggtatctc
     4621 aaagaccggc aatatctgtg gagcggtgtc aggggcagtc atggtgattg gcctgaaata
     4681 cggaaaaacg aagatgggtg atgatgcagc cactgagaag acaagagcgc tggtccgaaa
     4741 attcatgcag gaattcacaa aacgccatgg ttcggtctgc tgtacggaac tgctcggcta
     4801 taatcttggc aaaccggatg agtatgaaaa ggcccgtgac agcaaactgt tcgtgacgaa
     4861 atgtccacaa ctggtcgaag atgctgctgc aattcttgaa aaatttctct gaacataaaa
     4921 acaggtgacc atcaatggaa aaacccgcaa aacaactctc ctttctcgac cgatacctga
     4981 cgctctggat cttccttgcc atggcagccg gtatcggtat cggatacttc gctccccaag
     5041 tgcccgcaat gatcaccggt ctctcggtcg ggaccacatc aatcccgatt gccctcggcc
     5101 ttatcctcat gatgtacccg ccgcttgcaa aagtgaagta cgaagaactc attaaggtat
     5161 tccagaatac cagagtgctg ggtttatcac tcgtccagaa ctggattgtc gggccggtcc
     5221 tgatgtttgc ccttgcagtc atctttctcc gcgaccagcc cctgttcatg tacggcctga
     5281 tacttgtcgg tattgcccgc tgtatcgcaa tggtgattgt ctggaacgat ctcgctgaag
     5341 gggactgcga gtactgcgcc gcaattgttg gcctgaactc ggtcttccag gtcttcttct
     5401 actcagttta tgcctggttc ttcataacgt acctgccccc gctgctcgga atcgggaaag
     5461 gttctgcggt ttcgattacg attctccaga tagccgggag cgtctttgtc tatctcggca
     5521 tcccgttcat tgccggcatg gtcacccggt atgccctgct ccgcgtgaag tcgaaagcgt
     5581 ggtacgaaca ggttttcatc ccaaaaattt cacccatcac gcttatcgca cttctcttta
     5641 cgatcatcgt aatgttctca cttaagggag aatacatcgt caacgtgcct ttcgacgtag
     5701 tacgggtagc gatcccgttg ctcttctact tcatcatcat gttctttgcg agtttctgga
     5761 tgtcctatcg tctaggtatc gattaccgga aatccgcctc gctctcgttt accgcagcat
     5821 ccaacaattt cgagctggcg attgctgttg cgatcgccgt ctttggcatt gcctcgccag
     5881 cggcatttgc tacggttatc ggaccacttg ttgaagtgcc ggtattgatc ggcctcgtga
     5941 acgtttccct ctggatcagg gacaggtggt atgggggaaa gcgtgataac tcgtgcagcc
     6001 cggtgcggta gtgaatgagc catcaaaaaa tatttttttt ctggattttg tgaaatccat
     6061 taacaacctt tgcaagttaa tatttcaatt ttttagacaa gacctttcct ttatgtacca
     6121 tgaccataaa ctaaccattt ggtaatcatc atggatggat atgcaggaaa tatagtctat
     6181 gccgatcttt cggcagagac ggttacaatc aggcccacac caccggaact taaaaaaatg
     6241 tatattggc
//
LOCUS       DASRLV010000065         2689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_584237,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000065 DASRLV010000000
VERSION     DASRLV010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584237"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="VFC43_02700"
     CDS             <1..850
                     /locus_tag="VFC43_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HZK30131.1"
                     /translation="AVAAYEFTTLTVVPGALEHKGAKIQLLDIPGLIAGAAMGKGRGK
                     EVIGVVRGADIIIILVDVFNERHFDVLLKELYDAGIRINVPKPDITIKKASHGGVRLH
                     AVGTLNLDIEEVRSILSESKMMNADVLVRGGATQDDLIDAIQGNRVYVPAFIAVNKVD
                     LVDKERYLEIEHDIAGRFGSPPLMISAAAGYHLEEVKDAIYDCLGFIRVYLKPHGGEA
                     DLEEPLIVRTGSTVADVCTKLHRDFVQRFRYARVWGKSVKHPGQRVGLPHKLIDSDLL
                     TIIVEH"
     gene            complement(834..1952)
                     /locus_tag="VFC43_02705"
     CDS             complement(834..1952)
                     /locus_tag="VFC43_02705"
                     /EC_number="2.1.1.213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(10)-N(2))-dimethyltransferase"
                     /protein_id="HZK30132.1"
                     /translation="MDLIEATEGKTSFFVPAQDAAAHFPPGTAPVFFNRRMELNRDAT
                     ILLLSILRPSDYLDAMGASGVRGLRVAHECSVPVTINDRDPQGIDLIAHNACHANLDI
                     EIVQRDASALLSERSFDAVDLDPFGTPAMYVDAAVRGTRRFLFITATDTAPLCGAHLK
                     AGKRRYFAQPMNTDYHGEVGLRILLGFVVRETVKYDRGIEPLFCFAREHFVRLHVRLT
                     RGADAADETMKRIGYILQCKTCQYRTEHQGMFPDHVCCPHCKEKLTPVGPLWLGSIQH
                     KETLLQMRECLGEMQLGTKKDLLKLIDLCSDELPTSSYYDYHRLTKIVGCSPPGISTV
                     IAHIIAAGYPATRTHFSGYGIKTEAPLEIIFEAISARQ"
     gene            2014..2088
                     /locus_tag="VFC43_02710"
     tRNA            2014..2088
                     /locus_tag="VFC43_02710"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2049..2051,aa:Arg,seq:cct)
     gene            2159..>2689
                     /locus_tag="VFC43_02715"
     CDS             2159..>2689
                     /locus_tag="VFC43_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HZK30133.1"
                     /translation="MRIAGFFTITRPVNSLVAGLAAIIAYLIDTGTLIPQTLLLFCVV
                     ALVTAAGNVINDFFDAEIDAVNRPDRPIPSGAVTRTVARGFAVTLFLAGILVSFFTGF
                     LCIAIAIINSLILVAYAGTLKRTPLFGNITVAYLSASIFLFGGALNGWDGLVHIVPIA
                     MITFFAMLSRELVKDAE"
BASE COUNT          609 a          707 c          707 g          666 t
ORIGIN      
        1 tgcagtagct gcgtatgagt ttactacact cacggttgtc cctggtgccc tggagcacaa
       61 aggggccaag atccagctgc ttgatattcc gggacttatc gccggggctg caatgggaaa
      121 aggaaggggc aaggaagtga tcggcgttgt acgcggcgca gacatcatca ttatccttgt
      181 cgatgtgttc aatgagcggc atttcgatgt cttattaaaa gaactctacg atgccggcat
      241 ccggatcaat gtccccaagc ccgatatcac aataaaaaag gcatcacacg ggggtgtccg
      301 tctccacgct gtcggcaccc tcaaccttga tatcgaggaa gtgcgttcca tcctgtccga
      361 gagcaaaatg atgaatgctg atgtgcttgt ccgtggcggt gcaacccagg atgacctgat
      421 cgatgccata cagggaaacc gcgtttatgt ccctgcattt attgcggtga acaaggtcga
      481 tctcgttgat aaggaacggt acctcgagat tgaacatgac atcgccggac ggttcggcag
      541 tcccccgctc atgatatcgg cagcagccgg gtaccacctc gaagaagtca aggatgcgat
      601 ttatgactgt ctcgggttca tccgtgtgta cttaaaaccc cacgggggag aagcggacct
      661 tgaagaaccg ctcattgtcc ggacaggcag cacggtagcg gatgtctgta ccaaacttca
      721 ccgggacttt gtccagcgtt tccgctatgc acgggtctgg ggaaaatcgg tcaaacatcc
      781 cggacaaagg gtgggcctgc cccataagtt aatcgattcc gacctgctca cgatcattgt
      841 cgagcactga tcgcttcaaa aattatctct aagggggcct ctgtttttat cccgtaaccg
      901 gagaaatgtg tccgggtagc aggataccct gcagcaatga tatgggctat caccgtgctg
      961 atgccgggtg gcgagcaacc gacgattttt gtgagccggt gataatcgta atagctggat
     1021 gtcggcaatt cgtcagaaca gagatcgata agtttcagta aatctttttt tgtaccaagt
     1081 tgcatctcac ccaggcattc cctcatctgc aagagggttt ctttatgctg gatgctcccg
     1141 agccagagag ggccaaccgg ggtgagtttt tccttacagt gagggcagca aacatggtca
     1201 ggaaacattc cctggtgttc ggtgcggtac tggcaggttt tgcactggag gatgtacccg
     1261 atccgtttca tggtttcatc cgcggcatcg gctccccttg tcaaccgtac gtggaggcgg
     1321 acaaaatgtt cgcgggcaaa acagaacagc ggctcaatac cccggtcata cttcaccgtt
     1381 tcccggacaa caaaccccag caggatccgc agtcccacct ccccgtgata gtctgtattc
     1441 attggctgtg caaagtaccg ccttttgcct gctttcaagt gggccccgca taacggcgca
     1501 gtatcggttg cagtaataaa caggaacctc cgggtgcccc gtacggcagc atcgacatac
     1561 atcgccggag tcccgaaagg atcaaggtcc actgcatcaa atgaccgttc agaaagtaac
     1621 gcgcttgcat cccgctggac aatctcaata tccaggttcg catggcaggc attatgcgcg
     1681 atcaggtcaa ttccctgcgg gtcgcggtca ttgatggtaa caggaacaga gcactcatgt
     1741 gccaccctga gtccccggac tccggaagcg cccatggcat cgagatagtc ggaaggcctg
     1801 agtattgaca ggagaaggat cgtggcatcg cggttcagct ccatccgccg gttgaaaaag
     1861 accggtgcag ttcctggtgg aaagtgagcc gcagcatcct gtgccgggac aaaaaacgaa
     1921 gtttttcctt ccgttgcctc gatcaaatcc attgcgagag tattgttgca gcgcgcaaaa
     1981 cttatacata ttcctccaca atggatatgg gcagggcgag tggcttagcc cggacatagc
     2041 gtcagcctcc taagctgaat gccgggggtt caaatccccc ctcgcccgtc gcagatattg
     2101 agcggatttt ccccattttg gtgcatttta ttacttcatc tgccagatct catagttaat
     2161 gaggattgca ggttttttta ccatcacccg tccggtcaac tcgctcgttg ccggtcttgc
     2221 tgccatcatc gcgtacctta tcgataccgg gacactcatc ccccagaccc tgctcctctt
     2281 ttgtgtcgtg gcactggtca ctgctgccgg aaatgtcatc aatgattttt tcgatgcaga
     2341 aatcgatgca gttaatcgtc ctgaccgccc gattccttcc ggtgctgtga caaggacggt
     2401 cgcgaggggt tttgccgtta ccctcttcct tgcaggaatt cttgtgagtt tttttaccgg
     2461 ttttctttgt atcgctattg cgattataaa ttcactcatc cttgttgcat acgcgggaac
     2521 actaaaacgc acccccctgt tcggtaatat cacggtggca tacctttcgg caagtatttt
     2581 tctgtttggc ggagctctga acggttggga cgggctggtc catattgtac cgatcgcgat
     2641 gattacattc tttgcaatgc tgtcccggga actggtgaag gatgcggag
//
LOCUS       DASRLV010000066        11821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1074796,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000066 DASRLV010000000
VERSION     DASRLV010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 11821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11821
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074796"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VFC43_02720"
     CDS             <1..613
                     /locus_tag="VFC43_02720"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZK30134.1"
                     /translation="QAQIDACQAVVQHLGLDNGKDLFSYFLSPDHGFESHENIRQFMN
                     ERNIPLENTFRKACQVYETVKLQQISPYEGVDETLHLLQERGYSMGIVTDAHSRDATL
                     RLEKTGLLPFFCCMVSYDMVQVKKPAHDPFLLALEMMKTSPSDAMLIGDSPRRDIEPC
                     RHLGIWTVYARYGDRFFPDRSNVPADFIIDTLTELPEILLNFS"
     gene            764..1306
                     /locus_tag="VFC43_02725"
     CDS             764..1306
                     /locus_tag="VFC43_02725"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HZK30135.1"
                     /translation="MYVPTRIFFTKGVGVHKDRLASFELALRKAGVEKCNLVYVSSIF
                     PPKCKQISKSEGLKCLQPGGITFCVMARNETNEPNRLVSAAIGLALPSDSDEYGYLSE
                     HHAFGETQEKTGEYAEDLAATMLATTLGIEFDVNAAWSEREQTYKASGKIIKTQNVCQ
                     SAEGDKNGLWTTTIAVAVFL"
     gene            complement(1376..2140)
                     /locus_tag="VFC43_02730"
     CDS             complement(1376..2140)
                     /locus_tag="VFC43_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZK30136.1"
                     /translation="MAHTPFIIALSGKGGTGKTTISSLIIRSLIDQGETPVLAVDADP
                     NANLHEALGVTVHETLGCMREEAFTRHIPPGMNRHDYVKFRFRQALVEAKGFDLIAMG
                     RPEGSGCYCFANDLLSECITRLERDYLFIVIDTEAGMEHISRGTIGKPDMLLIVSDPG
                     ARGLRTITRIREIATQLGLEKEKIFIVFNRYKSVTAPVDFGIDTPIAIIPDDPLVEAA
                     DLAATPVSQVPASSPALGAVLELVRIIIKSAHEKPS"
     gene            complement(2160..3191)
                     /locus_tag="VFC43_02735"
     CDS             complement(2160..3191)
                     /locus_tag="VFC43_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30137.1"
                     /translation="MISSPHSLATGVGALPHTDPVVACDDVLAIFPGFVYVPTLPDRN
                     LLESIVFCDSEQLPGRMIRENRLLFDSTTDHATAIEKVYMDYVEGNYASYGFHPDYAS
                     GFIEMMSRDLSGAKVLKCQVTGPVTFGMQVVDADKRPIYYDSQLADLLSKMIALKARW
                     CEMEMRKRTGVKETLVVLNEPYFASLGSSVVPIDQSMVKSGWEDISALVEGGLGIHCC
                     SNTDWEFVLDLRPSVLSLDAYTTAKEFLLYSDSLITFMEQGGVVAWGIVPAEYKIFEP
                     ETPETLYQKYLAIRTQLCSSMPEQLFDAQSLITPSCGIRFADRSGALAIMKTAAQISQ
                     RVRHKACGE"
     gene            complement(3205..5106)
                     /locus_tag="VFC43_02740"
     CDS             complement(3205..5106)
                     /locus_tag="VFC43_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HZK30138.1"
                     /translation="MRTVTFLPSYRKIDVARGSTILDAAQRAGLNINVVCGGQGKCGK
                     CVVFVQSGKTVFDRQKYVRFFSEVELAKGACLACQTFVQGDLQVLIPESTFIQEQKIL
                     VDGRDEGIEFHPSVRKYYVELHSPTLTDPSPDLTRLLWSIQRSGGPVAEKIYVPLEVM
                     REIPNLLRHSDWKVTGTVALVPGGYRLVDLQENDTSKRIFGAAVDLGSTTVVVYLWDL
                     VRGKVVAVASNYNKQISCGEDILARVNFARKNGLEKLQTLAAESINMALTAASNTAGI
                     DREDIYEVVVAGNTVMTHMLLGIDPAYIIAEPYVPVVQRALSVAASRIGIAGNENCGL
                     FAFPAVSDFIGGDIIADILSCGMSKQEGISLLIDIGTNFEVVLGNREWMFSCAGAAGP
                     ALEGGEVLFGMRANPGAIEKITVDPFTLNTFYSTINNIKPRGITGSGLIDLLAELISA
                     CVIDRTGRINVSIENKRIRAGQHVPEFVVAWAGETDIGKDIIITENDIKNLIMSKASI
                     HAACVTLMKEAGVTRHDISTIYFAGAFGNYLDKKNATIIGLIPEIELNKIKNIGNGAV
                     TGANIALINRRERKTLDEIASRIAYIELNAEPSFMDEYTSSTFLPHTDLTLFPVVQKL
                     LESCRLRKR"
     gene            complement(5212..6546)
                     /gene="acsC"
                     /locus_tag="VFC43_02745"
     CDS             complement(5212..6546)
                     /gene="acsC"
                     /locus_tag="VFC43_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HZK30139.1"
                     /translation="MALKALDIYKLLPKKNCKECGDPTCLTFAMKLAGGKADLDLCPY
                     LDDQAKSILGATTRPPIRLIKLGVGERSFAIGEESVLYRHEKTFYHPPGLIFKLSDTQ
                     SAEEIKAVTIRVRDEVLTRVGTDLRFDGIAIENVSGSPSMYAQAVATVEENADLPLVL
                     MAGNTEALNAALVQVGSYQPLIHAATGENFRNMCEIAKKHNCPLAIKAHGLDDLNTLA
                     KECLADGVKKLVLDICPRSLGDFITLSTAIRQLAITRAVPDLGYPVFIDATHPSLQDA
                     AIALGIVKYASVIVSPPLSPASSKAALTLRQNIYTDPQKPIQMNPGLYRVGSPGKDAP
                     VLMTVNFSLTFFTLQGYLESSRVPCFLLIVDTEGLSVLTAVAAGKLNETLVRDSILKF
                     NVENEVSHKTLIIPGYSAPLSGRIEEATGWKVMVGPRDAADIGEFLHEKWKK"
     gene            complement(6549..7505)
                     /locus_tag="VFC43_02750"
     CDS             complement(6549..7505)
                     /locus_tag="VFC43_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HZK30140.1"
                     /translation="MGYDLNFGWTGTVGHVVIGATSAEGGTRKTSYTLGGGMSLPYLD
                     NSPSSPSPLVALEICDNPEFWSPIIRRFCGDLVYNTSDWAKTAEKVYGADLVRLYLTS
                     TRVRNFSDISSVGKTVASVLDATALPLIIEGSNEPAIDSDVFQKCGETGQGERLLLGT
                     AEAGRYRSIAAAALAYDHSLIAQSPIDVNLAKQLNILLKEIGVQRDHIMIDPYTGALG
                     YGFEYSYSAMERIRFSALKGDTDLAMPIICSAIDSLNIKEVREADPALQDEMALQWEF
                     YTGIAAAVAGAEIICVRHPKTVGMLKNAFACMKSDAGFYGVK"
     gene            complement(7515..9566)
                     /gene="acsB"
                     /locus_tag="VFC43_02755"
     CDS             complement(7515..9566)
                     /gene="acsB"
                     /locus_tag="VFC43_02755"
                     /EC_number="2.3.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha/beta"
                     /protein_id="HZK30141.1"
                     /translation="MIRVKNPMTDVEVAKKKGDDQLKIMIGRMQQSFSYAETAYHLPI
                     SFALTGIAVHDQKAALDVFSRTGSNPLVALESILAEKKAAEGKEPSPYTGFIDDTVIR
                     KLGYSLVDGSILGLALVVGTPESADSAAAICRELQEKYMLTFLAGNVISSLSHAGVKL
                     GLEYRLIPLGSTPAYGVHFVDIIARVAMMFGGVTPGDSHRLLTYAAERAKAIVIVFPG
                     LSDQEIALVDGMRVLGFPILSVGEYTGGDWIPALPEAVVRRGMEIKGIRVNITAIPIP
                     MGCSPAFEGKSIRKEEMYVEFGGGRSPAFELLLMCPAAEVKDGQVTVIGPEIDAMKEG
                     GAYPLAIIIHVAGKMMKQDYEPVLERRIHNFVNYGEGSWHVAQRDIIWVRISKEVVAK
                     GVKIEHIGKLLASKFRMDFPQLLDAVSVTLITDEAKVLAAKKDAEAVYDERDARIRGM
                     RDADVNTYYSCTLCQTFAPNHVCVITPEHPALCGAISWLDGKIAYEIAPSGANQPIVK
                     GTAINALNGEFEGVNKFVKKASHGEIDRCSLYSVMEYPMTCCGCFECIALMIPEVNGI
                     MVVNREYKGVTPSGMTFSTLAGTIGGGAQTPGFAGISKNYILSDRFLQGEGGIERLVW
                     IPRQLKEELKARLEKKLSEQNKPDLFEKIADETQATSLEDLITFLERVKHPALTMKPL
                     V"
     gene            9691..10590
                     /locus_tag="VFC43_02760"
     CDS             9691..10590
                     /locus_tag="VFC43_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30142.1"
                     /translation="MDNRYLKNYFFSIAVFMAAICIIQPVFAQNGTMTISYRGSGGYY
                     VGDTIVFDGKNTVGNNTLLKITGPNLPTSGVPVYDLGGAPGSGNTVPGDTDGTWRFAW
                     SSANTKGIDKLVTARYTVTAFDLANPRLTASTSFFLKKPEFYINPQPGPVLPGDYIQL
                     SGVAEKGVTNVKIDITDSTGKILHTHTAPVSADGFFNYEFRGDMQPGQYFVSAGNPSM
                     KDILKSTITVAAPAGVVPTVTPSPISTPLENETLTTTISPTLTQKVTPRPSAAPMSPV
                     TVIAGLVFTGIVAISWSNRQRKQ"
     gene            10741..>11821
                     /locus_tag="VFC43_02765"
     CDS             10741..>11821
                     /locus_tag="VFC43_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZK30143.1"
                     /translation="MPNTPGSLHRAAEIIQKYEGNINRIQYDRRIDPSTVFYEVTASD
                     EAFGQITQDLTQIGYLQTSLKPLDFLKICVYLPHKPGALYEFLNFTTSAGANIAFIDF
                     DDKGRHPDRLTVSLNLEQSSVVDHLLDQLKSCYRLDIIEYDTTGKQLDDTVFYVRYAQ
                     AIRELIGDSEENFLLSFLADTNHIVQELMDRGNDPRKVFESVLLTGQTIWATTHEGFY
                     ADIQKFRITETTTLFCFQMPCGGNIFLITSPTEKVLIDTGYGIYHDDVIKMLSGCGLA
                     DKYEISRIIVTHADADHCGAAGYFTAPVLLHEGTLSIIQKNNRAYGSRRENSVLEEFY
                     TKMINLFSKFNSQSKIECFSKKEDRM"
BASE COUNT         3100 a         2957 c         2684 g         3080 t
ORIGIN      
        1 ccaagcccag atcgatgcat gtcaggcagt tgtacaacac ctgggcctgg ataatggaaa
       61 agatctgttc tcgtattttt tatcacccga tcatggattt gaatcacatg aaaatattcg
      121 ccagttcatg aacgaacgaa atattccgct tgagaatacg tttcgtaaag cctgtcaggt
      181 gtatgagacc gttaaacttc agcagatatc accgtatgaa ggcgttgatg agacattaca
      241 cctgctccag gaacggggat attccatggg aattgtgacc gatgcacatt cccgggatgc
      301 aacactgcgt ctggaaaaaa ccgggttact cccgtttttc tgctgcatgg tctcgtatga
      361 tatggtacag gtgaaaaaac cggcacacga cccattcctt ctggctctcg agatgatgaa
      421 aaccagcccg tctgatgcaa tgctgatcgg tgacagcccc agaagggata tcgagccctg
      481 caggcatctg ggcatatgga cggtgtatgc ccggtacggg gaccgtttct tccctgaccg
      541 ctcaaatgtt cctgccgatt ttatcatcga tactttaaca gaacttcctg aaattctcct
      601 gaatttttca tgagaagaga aatttttttg catttggctg ctatacacaa aggagcataa
      661 taacaggcgc gattagtgta tttccagcat taatcacgtg tatttttgtt tttaaaccgg
      721 atttgaccaa aacttaaaga aagaaaaaga tataataaaa actatgtacg ttcccaccag
      781 gatctttttc acaaaaggtg taggtgtgca taaagaccgt cttgcatctt ttgaacttgc
      841 attgagaaag gctggtgttg aaaagtgtaa tctggtatac gtttcaagca tctttcctcc
      901 aaaatgtaaa caaatatcaa aatcggaagg attgaaatgc cttcagccag gcggcattac
      961 cttttgtgtc atggcaagaa atgagaccaa cgagcctaac cgactcgttt cagcagcaat
     1021 tggtcttgcc ctgcccagcg attcggatga gtacgggtat ctgtctgagc accatgcttt
     1081 tggcgaaaca caggaaaaaa ccggtgaata tgcagaggat cttgcagcaa cgatgctagc
     1141 aacaacactt ggtatcgaat tcgacgtgaa tgcggcatgg tcagaacgcg aacaaaccta
     1201 taaagcgagc ggaaaaataa tcaagaccca gaatgtctgc cagtcagcag aaggggataa
     1261 aaatggtctc tggactacaa caattgctgt ggcagtattt ttataactga ttgcaacgga
     1321 aatacctttt ttccaacacc agtttgtttt tttattaccc gagtcccgat acctgttaag
     1381 aaggtttttc atgcgctgat ttgattatta tccttaccag ctcaaggact gcacccaggg
     1441 ctggactgct ggcaggaacc tgggaaaccg gtgttgcagc aagatcggca gcctcgacca
     1501 gcggatcatc agggattatc gctattggtg tatcgatccc aaaatcgacc ggcgctgtta
     1561 ccgatttgta ccggttaaaa acaataaaaa ttttttcctt ttcaaggccc agctgggttg
     1621 caatttcccg gatacgggtg atggtccgca accctcgtgc accaggatcg cttacaatca
     1681 acagcatatc cggctttccg attgttcccc tgctgatatg ttccatacca gcctcggtat
     1741 cgataacaat gaacaggtaa tcacgctcaa gccgggttat acattccgat aacagatcat
     1801 tggcaaaaca atagcacccg ctaccctccg gccggcccat cgctataaga tcaaaaccct
     1861 ttgcttctac cagagcctgg cggaaacgga acttcacata gtcatgccgg ttcatcccgg
     1921 ggggaatatg ccgggtaaat gcctcttccc gcatgcatcc cagcgtttca tgaacagtta
     1981 ctccaagggc ttcgtggagg tttgcattcg gatctgcatc aactgcaaga acgggtgttt
     2041 ctccctggtc aataagcgaa cggataatca gggaacttat cgtcgttttg ccggtcccgc
     2101 ctttgccaga taatgcaatg ataaaaggag tgtgtgccat aaaatcttcc ggaacagggt
     2161 cattctccgc aggctttgtg cctgacacgc tgggagatct gtgcggcagt tttcatgatt
     2221 gccagagccc cgctcctgtc ggcaaaccgt ataccgcagc tgggggtgat aagggactgt
     2281 gcatcaaaaa gctgctccgg catgcttgaa caaagctggg tcctgatagc cagatacttt
     2341 tggtacaggg tttcaggagt ctcgggttca aagattttat attcggcagg aactatcccc
     2401 catgctacca cacctccttg ttccatgaag gtgatcagcg aatccgaata caacaaaaat
     2461 tcctttgcag tagtgtatgc atcgagggaa agaacggaag gcctgagatc caacacgaac
     2521 tcccaatccg tatttgagca gcagtggatg ccaagcccgc cttctaccag cgctgagata
     2581 tcctcccatc cggattttac catactctgg tcaattggca ccacggatga accaagggaa
     2641 gcaaaataag gctcgttcag aaccacaagt gtttctttta ctcctgttcg ttttctcatc
     2701 tccatctcgc accatcgcgc cttgagagcg atcatttttg aaaggagatc cgcaagctga
     2761 gaatcatagt agattgggcg cttgtccgca tccaccacct gcatgccaaa ggtgaccggg
     2821 cctgttacct ggcatttcag cacctttgca ccggaaaggt cacgggacat catttcaata
     2881 aaaccggatg cataatccgg gtggaatcca taggaggcat aattgccctc aacataatcc
     2941 atgtacacct tttctatggc cgttgcgtga tcagttgtac tgtcaaaaag gagccggttt
     3001 tcccgtatca tccgtcccgg caactgttca gagtcgcaaa agacaatact ttccagcaaa
     3061 ttgcggtccg ggagagtagg aacatacacg aatccgggga atatcgctaa aacatcatca
     3121 catgctacaa caggatccgt atggggaagt gcaccaactc ccgttgcaag ggaatgcggt
     3181 gaagagatca tctttgacac gtgatcacct tttcctgagc cggcaggatt ctaaaagttt
     3241 ctgcaccacc gggaacaagg taagatcggt atggggcaaa aaagtgcttg aggtatattc
     3301 atccatgaaa gacggttcgg cattcagttc aatataggca attctggacg cgatttcatc
     3361 aagtgttttt cgctcacggc ggttaataag tgcaatattt gcacccgtta ctgccccgtt
     3421 cccaatattt tttatcttgt ttaattctat ctccggaatc agtccgataa tagttgcatt
     3481 ttttttatcc agataattac caaatgctcc cgcaaaatag attgtagaga tatcgtgccg
     3541 tgtaacacct gcttctttca tcagggtgac gcaggctgca tgaattgagg ctttgctcat
     3601 aatcaggttc ttgatatcgt tctccgttat gataatatcc ttgcctatat ctgtttcacc
     3661 ggcccaggca acaacaaatt ccggaacgtg ctggcctgcc cggattcgtt tgttctctat
     3721 gctgacattt atccgtccgg tccggtcgat aacgcatgca gatatgagtt cagcgagaag
     3781 atctatcaac ccggagccgg taattccccg gggtttgatg ttgttgattg tactataaaa
     3841 tgtattgaga gtaaaaggat caacggtaat cttttcgatc gcaccagggt ttgcccgcat
     3901 gccaaagaga acctctcctc cttccagtgc aggaccggca gccccggcac aggaaaacat
     3961 ccattctctg ttgccaagaa ccacttcaaa attggtgccg atgtcaatca aaagactgat
     4021 cccttcttgt ttactcatac cacaggatag aatatcggca ataatgtcac ccccgataaa
     4081 gtcactcact gcagggaagg caaagagccc gcaattttca tttcccgcta taccaatacg
     4141 ggacgcggca acggaaagtg cccgctggac cacaggcaca tagggttctg cgatgatata
     4201 cgccggatct atcccgagaa gcatatgggt cataacggta tttccggcga caacaacttc
     4261 gtaaatatct tcgcggtcaa ttcccgcggt atttgaagca gctgtgagag ccatattgat
     4321 gctctctgcg gcaagggtct gtagtttttc aagcccgttt ttccgtgcaa aattcacacg
     4381 ggcaagaata tcctccccac aactaatctg tttgttatag ttcgaggcta cggcaacaac
     4441 cttgcccctg accagatccc agagatacac gaccacagtc gttgaaccca ggtcaaccgc
     4501 agcaccaaag atccgttttg atgtgtcatt ttcctgaaga tcaacgagac gatatccccc
     4561 gggaacaagc gcaactgtcc cggtaacctt ccagtcacta tggcgaagaa gattggggat
     4621 ttcccgcatt acttccaggg gaacatatat tttttcagca acaggtcccc cgctccgctg
     4681 gatactccac aaaagccggg taaggtccgg cgacggatcg gtaagggtgg gcgagtgaag
     4741 ttcgacataa tatttcctga ctgaagggtg gaactcgatt ccctcatctc ttccgtcaac
     4801 aaggattttt tgttcctgga tgaatgtgct ttccggaatg agcacctgaa gatctccctg
     4861 gacaaaggtc tgacatgcaa gacatgcccc ctttgcaagc tctacttcac taaaaaaacg
     4921 cacatatttc tggcggtcaa acactgtttt tcccgactgg acaaaaacga cacacttgcc
     4981 gcatttgcct tgtccgccac agaccacatt gatgttgagc cctgccctct gtgctgcatc
     5041 cagaatcgta cttccccggg ctacgtcgat cttgcgataa ctgggaagaa aggttacggt
     5101 acgcatgatt acctgtttct atacctacac atacggagag agatgagaaa tattctccgg
     5161 taattattgt cgcgcatttt tttagcacca aaaaatgtac ttcacgggtt cttacttctt
     5221 ccatttttca tgaaggaatt ccccgatatc cgctgcatcg cggggcccga ccatcacttt
     5281 ccagccggtc gcttcctcta tccttcccga gagcggtgcg gaatagcctg ggatgatcag
     5341 cgtcttatga gagacttcat tctctacatt aaatttcagg atcgaatcac gcacgagcgt
     5401 ttcattgagt tttcctgcag ccacggcagt caggacagaa agcccttcgg tgtcgacgat
     5461 cagaaggaaa cagggaacac gggaagattc aaggtatccc tggagggtaa aaaacgtgag
     5521 tgaaaaattc acagtcatca ggacgggtgc gtccttgccg ggagatccga cgcggtacag
     5581 tccggggttc atctggatgg gtttctgcgg atcggtgtaa atattctggc ggagcgtgag
     5641 tgcagccttt gaagatgccg gggatagcgg aggcgataca atcaccgagg catatttaac
     5701 aattccaagc gcaattgcgg catcctgtaa ggatggatgg gtggcatcaa taaataccgg
     5761 atacccaagg tcgggcactg cccgggtaat ggcgagttgc cgtatagccg tcgaaagggt
     5821 gataaagtca cccagtgaac gcgggcagat atccaggacc agtttcttta caccgtcagc
     5881 caggcactct tttgcgagag tgttgagatc atccaggcca tgagctttaa tcgccagggg
     5941 gcaattatgt tttttggcga tctcacacat attcctgaaa ttttctcctg tcgccgcatg
     6001 gatcaggggc tggtaacttc caacctggac aagggctgca ttcagtgctt cggtgttccc
     6061 agccatcagg accaggggaa gatcagcatt ttcctcgact gtggcgacgg cctgcgcata
     6121 catggatgga gaaccgctta cattctcaat cgcgatcccg tcaaaccgca ggtctgtacc
     6181 taccctggtc aatacctcat ccctgaccct gatagttacc gctttgatct cttcagccga
     6241 ctgtgtatct gatagtttaa atatcaatcc gggagggtgg taaaaggttt tttcatgacg
     6301 atataaaact gattcttccc caatagcaaa cgatcgttcc cctacaccca gtttgatcaa
     6361 tctgatgggt gggcgcgtgg ttgccccgag aatggatttt gcctggtcgt ccagatacgg
     6421 gcagagatcc agatccgctt tcccaccggc aagtttcatg gcgaacgtga ggcaggttgg
     6481 atcaccacat tccttacagt tctttttagg caggagtttg tagatatcca gcgctttgag
     6541 tgccatgatc atttcacccc gtaaaaaccg gcatcgcttt tcatgcaggc aaacgcgttt
     6601 tttaacatgc caaccgtttt tgggtggcgc acacagataa tctcagcacc ggcgacagct
     6661 gctgcaatgc cggtgtaaaa ttcccactgc agggccattt cgtcctgcag ggctgggtca
     6721 gcttcccgga cttctttgat atttaacgaa tcaattgctg aacagattat tggcatggcc
     6781 aggtcagtat cccccttcaa tgcggaaaac cggatccgtt ccatagccga ataggaatac
     6841 tcaaacccgt aaccgagtgc cccggtatag ggatcgatca tgatatgatc ccgttgcact
     6901 ccgatctcct tcaggaggat attgagttgt tttgcaaggt ttacatcgat aggagactgg
     6961 gcaataagcg aatggtcgta cgcaagagcg gcagcagcta tactccggta cctgcctgcc
     7021 tctgcagtcc cgaggagaag tctttccccc tgaccggtct caccacattt ctggaaaaca
     7081 tcgctgtcaa ttgcgggttc attactgccc tcaatgatta acgggagggc tgttgcgtca
     7141 agcaccgatg caacggtttt gccaacagag gatatgtcag aaaaattccg gacccgtgtg
     7201 ctcgtcagat acaggcggac cagatctgcc ccatagactt tttcagcagt ctttgcccag
     7261 tcagacgtgt tataaacgag atcaccacag aatcgcctga tgatcggtga ccagaattcc
     7321 gggttatcac agatctccaa tgcaacaaga ggagagggag acgacgggct gttgtcaagg
     7381 tacggaaggg acatccctcc ccccagggta taggatgttt tccgtgttcc cccttctgca
     7441 ctggtagcac cgattaccac atgaccgaca gtacctgtcc atccaaagtt cagatcatat
     7501 cccatgctgc tgcctcacac caggggtttc atcgtcagtg ccgggtgttt cacacgctca
     7561 agaaaggtaa tcaggtcttc aaggctcgtt gcctgggttt catcggcaat cttttcaaaa
     7621 agatctggtt tgttctgctc ggaaagcttt ttctcgagcc tggctttaag ttcttctttc
     7681 agctgcctgg gaatccatac cagccgttca ataccgcctt ctccctgtaa aaaccggtct
     7741 gagagaatat agttttttga tataccggca aaccccggtg tctgtgcccc tccaccaatc
     7801 gttcctgcaa gcgtggaaaa cgtcattccg gagggggtga cacctttgta ttcccggttc
     7861 accaccatga ttccgtttac ttcgggaatc atgagggcaa tacactcaaa acacccgcag
     7921 caggtcatcg gatattccat tacgctgtag agtgaacagc ggtcaatctc accatgactt
     7981 gcttttttga caaatttgtt tacgccttcg aattccccgt tcagggcgtt tatagcggtt
     8041 cccttcacta tgggctgatt ggcaccggag ggggctatct catatgcaat ttttccatcc
     8101 agccagctga tagctccgca gagggccggg tgttccggag ttataacaca gacatgattg
     8161 ggagcaaaag tctggcagag cgtgcaggaa taatatgtat ttacatctgc atcccgcata
     8221 ccacggattc gtgcatctct ttcatcgtac accgcttctg catctttttt tgctgcaagt
     8281 acctttgctt catcggtgat cagggtaacc gataccgcat ccagcagctg gggaaaatcc
     8341 atgcggaact tgcttgccag gagctttcca atatgctcta tcttcactcc tttggccaca
     8401 acctctttgg agatccgcac ccagatgata tcccgctggg caacatgcca ggatccttcc
     8461 ccgtaattga caaagttatg aatccgtcgc tccagtaccg gctcgtaatc ctgtttcatc
     8521 atcttaccgg caacatgaat gatgatagca agaggatatg cacctccttc tttcatcgca
     8581 tcaatctcgg gtccgatcac agttacctga ccgtccttga cctcagctgc aggacacata
     8641 agcaataatt cgaaagcagg agaccttccc ccgccaaact ccacatacat ctcctctttc
     8701 cggatactct ttccctcaaa tgcaggtgaa caccccatcg ggatggggat tgcggtgata
     8761 ttcacccgta tgccttttat ttccatgccg cggcggacaa ctgcttcagg aagtgctggt
     8821 atccagtccc cgcccgtgta ttctccaacg gaaagtatgg ggaagccaag cactctcatc
     8881 ccatctacca gtgcaatttc ctgatcagac agacctggaa atactattac aatggctttt
     8941 gcccgttctg cagcatacgt taaaagacgg tgtgagtccc cgggcgttac cccgccaaac
     9001 atcatcgcaa cccgtgcaat gatgtcgaca aaatgaaccc cgtaggcggg tgttgagcca
     9061 aggggtatga gacggtattc cagccccagc ttcactcctg catgggaaag ggatgagatg
     9121 acatttcctg caaggaaggt tagcatgtat ttttcctgaa gttcgcggca tatcgcagca
     9181 gcactgtcgg cactttctgg ggttcccaca acaagggcaa gtccgagaat gctgccatcg
     9241 accagagagt acccgagctt acggattaca gtgtcatcga taaacccggt atacggagag
     9301 ggttccttgc cttcggcagc ctttttttct gcaagaatgc tttccaaggc taccaggggg
     9361 ttactaccgg tcctcgaaaa tacgtcaagt gccgccttct ggtcatgtac agctattccg
     9421 gtcagcgcaa acgaaatggg aaggtgatat gcggtttcag cataagagaa ggattgctgc
     9481 atcctgccga tcattatttt taattgatca tcaccttttt ttttcgcaac ttcgacatcg
     9541 gtcatcgggt ttttcactct gatcatgggt tgattggtag aacacatata gtttcgtcaa
     9601 tcgataaatc ccggcaaatt ttcaaaacca atccgtacca aaaacattta acttcaatta
     9661 cgatattctg ttttagagga gactaaaaaa atggataata ggtatttaaa aaactatttt
     9721 ttttcaatcg cagtgttcat ggcagcaatc tgtatcatcc agccggtgtt tgcccaaaac
     9781 gggaccatga ctatctctta tcgtggatct ggaggctatt acgtaggaga caccattgtt
     9841 tttgatggga aaaataccgt aggaaacaac acattattaa aaattaccgg cccgaacctc
     9901 cccacgtctg gcgttcccgt gtatgatctg ggaggtgctc ctggttcagg caacacggtt
     9961 cccggggata cagatggaac atggagattt gcatggtcaa gtgcaaatac caaaggaatc
    10021 gataaactcg tgactgcccg ctacacggtt actgcatttg atcttgcaaa cccccggcta
    10081 actgcctcaa cttcattttt tttaaaaaag cctgaatttt atatcaatcc acaaccaggc
    10141 ccggtactcc cgggagatta catccagctg agcggggttg ctgaaaaggg tgtcacgaat
    10201 gtaaaaatcg atatcaccga ttcgaccgga aaaattctgc atacccatac cgctccggta
    10261 agtgcagatg gttttttcaa ttatgagttc cgtggggata tgcaaccggg acaatatttt
    10321 gtgagcgcgg gtaacccgtc tatgaaagat attctcaaaa gtaccataac cgttgcagct
    10381 cccgccgggg tagtcccaac cgttactcct tcccccattt ctactccctt ggaaaatgaa
    10441 actttaacta ccacaatctc acccacactt acacaaaagg tcacccccag accttcagca
    10501 gcacccatgt cacctgtaac agttattgca ggtcttgttt ttaccggtat cgtagcgata
    10561 tcctggtcaa accgccaaag aaaacaataa ccctcgaatt attattattt ttaaaacctg
    10621 aattgttaaa aaattttcag acattatatg aaacctacca taatatatat cggaattccc
    10681 ggtcaatact actagcacga ctggtggata tctttgaaaa aattctcttt tattgcacat
    10741 atgccaaata ccccgggatc actccacaga gctgcagaga tcattcagaa atatgaggga
    10801 aacatcaacc gcattcaata tgatcgacgt atcgatccct cgacggtatt ctatgaagta
    10861 acagcatctg atgaggcatt cgggcagatc acccaggatc ttacccaaat aggatatctt
    10921 cagacgtcat taaaacccct tgatttttta aagatctgcg tgtatcttcc ccacaaaccc
    10981 ggagctctct atgagttcct gaatttcacc acatcagcag gagcgaatat cgcttttatt
    11041 gattttgatg ataaaggaag acaccctgat cgccttaccg taagcctgaa ccttgaacag
    11101 agctccgtgg tcgaccattt gttagaccag ctgaaatcat gttatcgcct cgatattatt
    11161 gaatatgata caaccgggaa acaactcgat gatacggtgt tttatgtcag gtatgcccag
    11221 gccattcgtg aactaatcgg tgactctgaa gagaatttct tactttcctt cctggctgat
    11281 acaaatcata ttgtgcagga actgatggat cgcgggaatg atccccgtaa ggtctttgaa
    11341 agtgttctcc ttacgggtca gacaatatgg gcaacaacac atgaagggtt ctatgcagat
    11401 atccagaaat ttcgaattac tgaaacaacc actctttttt gcttccagat gccctgcggg
    11461 gggaatatat ttttaattac atctcccacc gagaaagtcc tgattgatac cgggtacggt
    11521 atttatcatg atgacgtcat aaaaatgctt tccggatgcg gtcttgcgga taaatacgag
    11581 atttctcgaa tcattgttac ccatgcagac gcagatcatt gtggggctgc cgggtatttc
    11641 accgcaccgg ttcttctgca tgaaggtact ctttcaatca ttcagaagaa taaccgggcg
    11701 tacggatccc ggagggaaaa ctctgttctc gaagagttct ataccaaaat gatcaatctt
    11761 ttctcgaaat ttaattccca gtccaagata gaatgctttt caaaaaaaga ggacaggatg
    11821 c
//
LOCUS       DASRLV010000067         4129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_342486,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000067 DASRLV010000000
VERSION     DASRLV010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342486"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1310
                     /gene="rpoA2"
                     /locus_tag="VFC43_02770"
     CDS             135..1310
                     /gene="rpoA2"
                     /locus_tag="VFC43_02770"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A''"
                     /protein_id="HZK30144.1"
                     /translation="MPLQDKFNKKIEAAALPKKTKDQLHKFLDNKDISDSQFTQILER
                     VLKEYLSTRIEACEAVGVIAAQSIGEPGTQMTMRTFHYAGVAEINVTLGLPRLIEIMD
                     ARKEPSTPTMTVYLEPEYSNDRDRAREVSWQIEEAPLHEFGDITTDMENMHVIVHLNT
                     KVCDKRKISPMEIMEDAPKNIRDKRHYRDFEHEINEETATIIFSPKNKESYQNLFQLA
                     EHVKNVIVQGRDKIQRVVVRKEGGEYILYTEGSNIKDVFDVVGVDTTRTRTNNISECA
                     NVLGIEAAREAIIHEAVSTLNEQGIAVDVRHLMLVADMMCMEGEVKQIGRHGIAGEKE
                     SVLSRAAFEVTVNHLLDAAVANEIDELSGVTENVIVGQPIQLGTGDVKLIAVRQHDN"
     gene            1346..1636
                     /locus_tag="VFC43_02775"
     CDS             1346..1636
                     /locus_tag="VFC43_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30e"
                     /protein_id="HZK30145.1"
                     /translation="MDFNASLRRAIKTGNVILGQNNTEKCIKEGKAQMIVVAGNCPAN
                     FKSKLTGYENLFIHTFEGSSVALGKACGKPFMVSTLAIVTPGESDILSLKRA"
     gene            1638..2114
                     /locus_tag="VFC43_02780"
     CDS             1638..2114
                     /locus_tag="VFC43_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusA-like transcription termination
                     signal-binding factor"
                     /protein_id="HZK30146.1"
                     /translation="MVEVKLTEDCMRLISQFESLTGAGSRDCVVDERNSRLIFVINPG
                     DMGLAIGKKGASIKKASEVMGKKIEVVEYNSNPEQFIRNCFLPAQVTSILFEGEPDEQ
                     TVQVEVKDEDRGIAIGKDGKNIFKAKKLSQRQHNIADVQILQKPTDDQPADDLMQE"
     gene            2433..3422
                     /locus_tag="VFC43_02785"
     CDS             2433..3422
                     /locus_tag="VFC43_02785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZK30147.1"
                     /translation="MALNNRSKFVLGLLLYCLIAAVIALIMYSLQAYLSSWTSLDADR
                     FHQMAQVIINGSEPYVTYIDPKPPLLFFTVALMDILAPAGSIDILVMTVVNVICALLI
                     WRIGWEDYGCLAGYTAGLLYLISSVFTEGYFLFSEQFTVLFILIALILVREKAYIGAG
                     VFLGLACGFKQYAFLAVIPLLYLMWAHHDRRYYRLILPLVVIVGSMFAAIYLMYGEIA
                     GNNAVYYTFLIAPLYNSGNVTMFSTYVPETPIAYAANILFSFTIVIPTLLFAIVSAVH
                     RGFRWHNEKALGIMVLVLFSTFLVRQYLHYWILLMPFLALLACREFADTDRKS"
     gene            complement(3573..>4129)
                     /locus_tag="VFC43_02790"
     CDS             complement(3573..>4129)
                     /locus_tag="VFC43_02790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PGF-pre-PGF domain-containing protein"
                     /protein_id="HZK30148.1"
                     /translation="SASSEPGKDLLPAPGTVYEYTDLLPARYTSIQEVVISGSVPQLW
                     LNEHNLTPQDVVIYHLSGSTWTVVPTNLVKSDGGRSYFTAVSPGLGRFAVTGDPRGPA
                     VPRVMGQETPQGTFNDMVKTSATQAVSTVSNAPVVMQTPEVPAQPATQPSAGFSSRTI
                     VAGIFGIIILIGLALVVRRRKSDL"
BASE COUNT         1095 a          965 c         1020 g         1049 t
ORIGIN      
        1 accgtaaggt caaccggtgg aagaatcatc cagttccagt atggtgaaga cagcacagat
       61 cccacaaaga gcgcttttgg cgaccctgtc gatgtcaaag gtattgtaga gagtatctta
      121 aaagaggaag tctgatgccg ctccaggata agtttaacaa aaagatcgag gcagcagccc
      181 tccctaaaaa gaccaaggac cagctgcaca agtttcttga taataaagat atcagcgatt
      241 cccagttcac gcagattctg gaacgggtgc tcaaggaata cctgtccacg agaatcgagg
      301 catgtgaagc ggtcggggtt atcgctgccc agtctattgg agaacccggt acccagatga
      361 cgatgcggac tttccactat gccggggttg ctgaaatcaa cgtaaccctg gggcttccgc
      421 gcctcatcga gatcatggat gcaaggaagg aacccagtac tccgaccatg actgtttatc
      481 tcgagcccga atacagcaat gaccgggacc gggcgcggga ggtaagctgg cagattgagg
      541 aagcgcccct ccacgagttc ggtgatatca ccacggatat ggagaacatg cacgttattg
      601 tgcacctcaa cacgaaagtc tgcgacaagc gaaagatctc accaatggag atcatggaag
      661 acgcgcccaa gaatatccgg gacaaacggc actaccgtga cttcgagcat gagatcaacg
      721 aggagacagc gaccattatc ttctcgccaa aaaacaagga aagttaccag aacctcttcc
      781 agctcgccga acacgtcaag aatgtgattg tgcagggcag ggataaaatc cagcgtgtcg
      841 tcgtcaggaa agagggcggg gagtatatac tttatactga aggttccaac ataaaagatg
      901 tatttgatgt tgttggtgtg gataccacac gaacccgtac caataacatc agtgaatgcg
      961 cgaacgtgct cggcatcgag gcagcaagag aggcgatcat ccatgaagcg gtatccacgc
     1021 tcaacgagca gggtatcgca gtagatgtcc gtcacctgat gcttgtcgca gacatgatgt
     1081 gcatggaagg cgaggtcaag cagattgggc ggcacggtat tgccggtgag aaagaaagtg
     1141 ttctttcccg tgcggcattt gaagtgacgg tcaatcacct gcttgatgct gccgtagcaa
     1201 acgaaattga tgaactgagc ggtgtcaccg aaaatgtcat cgtcggacaa ccgatccagc
     1261 tcggcaccgg tgatgtgaaa ctcatcgcag tcagacaaca cgataattga aacgaacaaa
     1321 atttagaaaa ccagaggaat cgtatatgga ttttaatgct tcactcagaa gagccatcaa
     1381 aacgggaaat gtaattcttg gccagaacaa cacggaaaaa tgtattaaag aaggcaaagc
     1441 ccagatgatc gtagtcgcag gaaactgtcc ggcgaatttt aaatccaaac ttaccggtta
     1501 cgagaacctg tttatccata cttttgaggg ctccagcgtg gcactcggca aggcatgcgg
     1561 gaaacctttc atggtaagca cacttgctat tgttactccg ggcgaatcag atatcctctc
     1621 tcttaagagg gcatgatatg gttgaagtta aactgactga agactgtatg cgcctgatct
     1681 cccagttcga gagtcttacc ggtgcgggaa gccgggattg tgttgtggat gagcgtaact
     1741 cacggctgat ttttgtaata aatcccggtg atatgggact tgccatcgga aagaaagggg
     1801 cctcaataaa gaaagcctct gaagtaatgg gaaagaagat tgaggttgtg gaatacaata
     1861 gtaaccctga acagttcatc aggaactgtt ttttacccgc ccaggtaaca tcaatcctgt
     1921 ttgaaggtga accggatgaa cagacggttc aggttgaagt aaaggatgaa gaccggggaa
     1981 tcgcaatcgg taaggacgga aagaatatct tcaaggcaaa aaaactctca caacgccagc
     2041 acaatattgc tgatgtccag atccttcaaa aaccgactga tgaccagccg gccgatgacc
     2101 tgatgcagga ataatctttt tctcatttta acacagtccc gatcattccg tacacttttg
     2161 tttcaggaaa taacgctgaa tcttaaaatc gttacagact gtctttagtc attttacgca
     2221 aattcgtatc aggccggcat acttacacct gcccgacgat gaaacttcac aagatttaat
     2281 agcgctccct ttgcatattt actgaatatt tcgatgggta tctcccaaaa ccgcattttt
     2341 ttgttaaagt gcaggaagac tgccggaaat tggtataaaa tggagtattg catgaagatg
     2401 agtcttgagg aaatgtatgg agaatatcat caatggctct gaataacaga agtaaattcg
     2461 ttctgggtct tctcctgtac tgcctgattg cagcagtaat agcgctgatc atgtattccc
     2521 tccaggccta cctgagttcc tggacctcgc tggatgctga ccgttttcac cagatggcac
     2581 aggtgataat caacggatct gaaccttatg tcacttatat cgatccaaaa ccccccctgc
     2641 tgttttttac tgtcgccctt atggatattc tcgcaccggc aggttccatt gatattctcg
     2701 ttatgactgt cgtgaatgtt atctgcgccc tcttgatctg gcggattgga tgggaggatt
     2761 atggatgcct tgcaggatac acggccggcc ttctttacct gatctcatca gtttttactg
     2821 agggttattt tctgttttcc gaacaattca cggtcttatt catcctgatc gccctgatcc
     2881 tggttcggga gaaggcatac atcggtgcag gtgtgtttct cggtcttgcc tgcgggttca
     2941 aacagtacgc ctttcttgcg gtgatcccgc tgctgtacct gatgtgggct caccatgacc
     3001 gccgttacta ccgtctcatc cttccgcttg ttgttattgt cgggagtatg tttgccgcaa
     3061 tttatctcat gtacggggaa attgccggta acaatgcggt atactataca ttccttattg
     3121 ctcccttgta taactccggg aatgttacga tgttttcaac ctatgtgccc gaaaccccga
     3181 ttgcatacgc agcaaatatc ctttttagtt tcaccattgt catcccgacc ctgctttttg
     3241 ccatcgtgag tgctgtgcac agggggttcc ggtggcataa tgaaaaagct ctcggtatca
     3301 tggtcctggt attattttcc acgtttttgg tcagacagta tctccactac tggatcctgc
     3361 tgatgccgtt tttagccctg ctcgcatgcc gggaattcgc tgatacagac cggaaatcat
     3421 aaaacctgat aacacgacca ctatgaggat cagtcttaaa atttaaaaat atctttgcct
     3481 gccatgtctt gagggggtcc tgatgcaagt ttatttagag tttggataaa tttcatagtt
     3541 ccggtgcgaa cccgactggt taatttcagt acctacagat cggattttct tcttctgaca
     3601 accaatgcca gtccgatcag aatgatgatc ccgaatatcc cggcgactat agtccgagat
     3661 gagaaccctg ctgaaggctg ggttgcaggt tgtgccggta cttctggtgt ctgcattaca
     3721 acaggtgcgt ttgacacggt tgacaccgcc tgggttgctg aggtcttcac catatcatta
     3781 aaagtacctt gtggtgtttc ctgtcccata acacgcggga cagctggacc ccttgggtca
     3841 ccggtgacgg caaatcgccc taggcccgga gatactgcgg tgaagtacga ccgtccgcca
     3901 tcagatttta ccaggtttgt cggtacaacg gtccaggttg agcccgacag atggtatatg
     3961 accacatcct gcggggtcag gttgtgctcg ttcagccaca actggggtac gcttcccgat
     4021 atgacaactt cctgaatcga agtataccga gccggcagga gatctgtgta ctcatagact
     4081 gtgcctggtg caggtaagag atccttaccc ggctctgatg aagcagagc
//
LOCUS       DASRLV010000068         8058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1155323,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000068 DASRLV010000000
VERSION     DASRLV010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 8058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8058
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155323"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VFC43_02795"
     CDS             <1..900
                     /locus_tag="VFC43_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FlaJ"
                     /protein_id="HZK30149.1"
                     /translation="ALLGINAGVVFLLVGILMAPLGITGFIDDSNITLRDNDFSTFIR
                     SLGSIMGGQGTTAVYALGTIDKKSLTALEPLVNTVYSQMNLGLDNRQIWDKFIGDSGS
                     NLIYKYLNIYLDTVIMGGPPEPIGTVVGSSVLEQTLLREKKDMHARSFIVLLVPMHAA
                     MVGILVVLFRIMLTLTGSVSSMMNKFAATTAASGGGASSGGVSASSALGGMSMFTNFP
                     EKEMGNFVVIILIIITVGNIFAARIVGGGDRYMFYFYAALFCTITGLILLIAPIGVNI
                     FFSPEGLTNMGKTGAGALGMYLI"
     gene            897..2189
                     /locus_tag="VFC43_02800"
     CDS             897..2189
                     /locus_tag="VFC43_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30150.1"
                     /translation="MKESLRRIILFTVIVVTGSVMILFDVPLIFMIPLILAVGFVILL
                     LLGAITIADIRSALKTLNLQNLKKIKILKRLDEMKFFEKKGVQPDKKPAPPSAKAVTP
                     SSNTKKTGIGSHLHSFFTSLRSLGTVLKERSKRERKIEHINELLDKAVSEKVKGSALA
                     SAAGNGTKINPSHSTGASIPGPNDQTKDQDPFLSLSGDEFDISLLDGLDDQESPSRTS
                     SVDQVPSPLPSSGGPAIAITEPEIPLPSLDITSEADEILKKNEGEGLEEFKGLEGGES
                     IDQDFGELDNLSIEDIDLDIDMEEETPVASGTSSAKNETANPSPSSNPVKTIQTDWVD
                     SDAPKNAATGENEISRQAEMASFAGGASGTDEDLLSSLASDVKHVAKEQNTSLLRDLK
                     DFKAPATAIENELKEMYETLKSVSDPRKKVIPPGKGMK"
     gene            2259..3068
                     /locus_tag="VFC43_02805"
     CDS             2259..3068
                     /locus_tag="VFC43_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CheF family chemotaxis protein"
                     /protein_id="HZK30151.1"
                     /translation="MKEVPIKIQHNNEWIVVQLGIGDDRIVIPAPVSKEIMFKNVADL
                     AEKKNVLIITVKTDVETVYKILSVEKVLQVLKKFILGSCNAYRLMAYFMSPAIRGGVM
                     IKDAQWDKGSVVVVRTGIWFISATKQVCVPVNDVAAIEITKRDVQGKPTDVIRIDHLD
                     SGEVSSSLVLCPLSTLQVLYNYLKDTTKGMDMKGTELDSIDQQVAMLVYSGMDSHAIE
                     NMLNIPHKQLDAIYDKILNLGIGEVVTIRREIQLTTKGVRYISDATKSQTN"
     gene            3139..3501
                     /locus_tag="VFC43_02810"
     CDS             3139..3501
                     /locus_tag="VFC43_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZK30152.1"
                     /translation="MGKILIVDDTLFMRTLLKNILFSGGHVIAGEAGDGEEGIAKYKE
                     LKPDLVTMDIVMPKLNGIEALKAIKEFDPAARIVMCTAVGQEQMVKLAIKSGAKGYII
                     KPFQAPKVLEEVKNVLAS"
     gene            3507..4550
                     /gene="cheB"
                     /locus_tag="VFC43_02815"
     CDS             3507..4550
                     /gene="cheB"
                     /locus_tag="VFC43_02815"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HZK30153.1"
                     /translation="MVKVLIVDDSVFMRTIIRDMLAKESSIEIVGTASNGLDALEKIQ
                     ELNPDLITLDIEMPKMNGLEVLLELKKIKKHPKVLMLSSLTSKDAEMTHEAIRLGADD
                     FMLKPKDLPHIREISEELISKVKHLFTLTRSEIHHKPIPSQGSNADRVVLIGSSAGGP
                     QQVDTLLSMLSPNLPAGVIITQHMPVGFTAPLAERFNRIATMPVKETQNGDVVETGKI
                     LLSKAGFHTVISGVLGESGKTTGRIVHTTSPPVHGVRPAVDKTFESAAHVYGKNAVSV
                     ILSGMGNDAGLGAHAIKEAGGVSLICDEADCLVYGMARSAIKNNAVDLILPLRKLSNE
                     IERVVYSMEGTNV"
     gene            4543..6495
                     /locus_tag="VFC43_02820"
     CDS             4543..6495
                     /locus_tag="VFC43_02820"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HZK30154.1"
                     /translation="MSEFEAYRELFVAESRENHENLVKNLLLLELGSYQDAIDEIFRS
                     IHTLKGASASMGFANMEHLCHSMEDVFQLIRSGAAEISPDLVNLLLGCSDLIEQMLDD
                     IEAGGDSSSKNPAEQVETLKKWIEQRGGTQKAQNAVPAVPVEVKTSEEPEEDKKAENR
                     VEYEMQITISSECQMKDVRALLALGNLKSVGTILSMNPTFELIDEGKFDGRLELVIAS
                     DAGEDALKTAASGTDISEIMIRPARKPEITTAGDLPEGPKKDEAEKKQSQTPDKSREI
                     KNLRVDIHQLDHIMNLVEDLVINRGRLKQIAEHHKIKEMDEAIGMVDRSVSELQSLMM
                     NIRMIPLNHIFNRLPRVVRDVAQYDNKEVDFFIEGGETELDRSVMDGLNDPLLHLIRN
                     AVNHGIESPELREKAKKPRKGFVKLSAHRDRDNVIIELIDDGAGINIDKVKAKGIEKG
                     LITPEVAETFTTEQAIDLLFQPGFSTADKISDISGRGVGLDVVRRSIEALKGTIRVET
                     TPGKGSRFELLLPPTMAIVDVMIVRINDKRFGIPISSIVEVANFKRDETHQIGKGEAI
                     LLRDEVLQILWLNEMVGVSKACEILIVVQYQKRKCCIPVDIVEGKQEVVVKPLSGFIG
                     NTRGISGVTILGDGGVVPVIDVNTII"
     gene            6505..7116
                     /locus_tag="VFC43_02825"
     CDS             6505..7116
                     /locus_tag="VFC43_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HZK30155.1"
                     /translation="MKLSTVQADAMQELGNIGAAHAATTLSQMLGSTIEMSVPAIKVI
                     DLSQLGDYMGEESAAMVAFELQGDIPHGGYVLFYITRESAIRMTNTMLGMTETNRTLS
                     EMDESALLEVGNIMVSAFLDATAELLGFVMLPSPPALTIDMAHAAMQSLIAQMQEETN
                     EVLLFSTELSCEEYKIDSDIIMMPERSTLERIIALLENMMKTN"
     gene            7133..7624
                     /locus_tag="VFC43_02830"
     CDS             7133..7624
                     /locus_tag="VFC43_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HZK30156.1"
                     /translation="MPDSHSRNEQTSMIGIGEYRVGSFPMMTIGLGSCIGLTMYDPNQ
                     KLGAMVHIMLPESAGRKDRPGKYADTAIPLLVNELTTLGCKNRSIIAKIIGGACMFEY
                     FGTNLNIGERNAEKVRILLKEHHIALAAEDVGGKVGRSVTFLPANEGKVSIRRADGSS
                     TDL"
     gene            complement(7632..>8058)
                     /locus_tag="VFC43_02835"
     CDS             complement(7632..>8058)
                     /locus_tag="VFC43_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30157.1"
                     /translation="YLDNRDAGGAAGILNHLIKQYPDDLEPIYLGLRFSLLTKKKITY
                     HSFRKLASTKGMPVHTIYLFDVAFDLLNGEKMDALNRISEFEKEFPHLQFYATILKYL
                     ANDFFLNDEIQVNRAKKALLDSIDQYCSEEIRMKRPAPT"
BASE COUNT         2337 a         1734 c         1987 g         2000 t
ORIGIN      
        1 gcattgcttg ggatcaatgc aggggtagta ttcctgctgg tgggaattct tatggcaccc
       61 ctgggcatca ccgggtttat tgatgatagt aatatcaccc tcagggacaa cgatttttca
      121 accttcataa ggagtctggg atcgataatg ggcggacagg gcaccaccgc ggtgtatgct
      181 cttggtacta ttgataaaaa atccctcaca gcccttgaac ctctcgttaa tacagtctat
      241 tcccagatga atcttggcct ggataacaga cagatctggg ataaattcat cggtgattcg
      301 ggaagcaacc tcatttataa atatctcaat atctacctgg acacggtgat tatggggggt
      361 ccgccggaac caatcggaac ggtagtcgga tcttcggtac ttgaacagac acttctgcgg
      421 gagaaaaaag atatgcatgc acgcagtttt atcgtcctcc tcgtacccat gcacgcggca
      481 atggtgggaa tccttgttgt ccttttccgt ataatgctga cactaaccgg ttctgtttca
      541 tcgatgatga ataaattcgc ggcaacgact gccgcatccg gaggaggagc cagcagcgga
      601 ggagtatctg cgagcagcgc tctgggtggg atgtcaatgt tcaccaactt tcccgaaaag
      661 gagatgggca actttgtggt aattatcctg attatcatca ctgtcggaaa tatcttcgct
      721 gcacggattg tcgggggagg ggatcggtat atgttttatt tctatgcagc gttattctgt
      781 acaatcacag gactcatatt attgatcgcc ccaatcggag ttaatatatt cttcagcccg
      841 gagggactca cgaatatggg aaagaccggt gcgggcgcac taggaatgta tcttatatga
      901 aagaaagtct gcgcaggatt atccttttta ccgtcattgt agtgaccggc tcggtcatga
      961 tcctgtttga tgtgccttta atcttcatga tcccattgat cctcgcagtc gggtttgtaa
     1021 ttctgcttct cctgggtgca ataaccattg ccgatatccg atcggcacta aaaactctaa
     1081 acctccagaa cctcaaaaag atcaaaatcc tgaagcgtct cgatgagatg aaattttttg
     1141 aaaaaaaagg tgtacaaccg gataaaaaac cggctcctcc ctctgcaaag gcagttactc
     1201 cctcaagtaa tactaaaaaa accgggattg gatcacatct gcattcgttt ttcacatcac
     1261 ttcgttctct ggggactgtg ctgaaagaac gaagtaagcg ggagaggaaa atcgagcaca
     1321 taaacgagtt gcttgataaa gcggtgagcg agaaggtgaa ggggtctgca cttgccagcg
     1381 ctgccggaaa tggtacaaag ataaaccctt cccattcaac aggagccagc attcccggcc
     1441 caaatgacca gacaaaagac caggatcctt tcctgtccct ttccggagat gaatttgaca
     1501 tcagtcttct ggacggactg gatgaccagg aatctccctc caggacctcg tctgtggatc
     1561 aggtcccctc ccctttacca tcatcaggtg ggccggcgat tgcaatcaca gaaccggaaa
     1621 tccccctgcc atcgctcgat ataacctctg aagccgatga gattctcaaa aaaaatgagg
     1681 gggaaggcct tgaagaattc aagggtcttg aaggtggaga atcgattgat caggattttg
     1741 gggaactgga caacctgagt attgaagata ttgatctgga tatcgatatg gaagaagaga
     1801 ctccggttgc cagcgggact tcttcagcaa aaaatgaaac tgcgaatcct tcaccttctt
     1861 ccaatccggt aaaaactata cagactgact gggttgattc tgatgctcct aaaaatgctg
     1921 ccacgggaga aaacgaaatc tcaaggcagg ctgaaatggc ttcctttgcg ggaggggcct
     1981 ccggcactga cgaagatctg ttaagttccc ttgcatcgga tgtcaagcat gttgcaaaag
     2041 aacaaaatac cagtctttta agggacttaa aagacttcaa agccccggcc actgccattg
     2101 agaatgaatt gaaagaaatg tacgaaactc tgaaatctgt atcggatccg aggaaaaaag
     2161 taataccccc gggaaaggga atgaaataac cctgctataa attattatca ggttcaaaaa
     2221 gaaagtatca cccatgcacg atcaccaagc gaggatatat gaaagaagtc ccgataaaaa
     2281 tccagcataa caatgaatgg atcgtagtcc aactgggaat cggggacgac agaatcgtaa
     2341 ttcccgcccc ggtatccaaa gaaataatgt tcaagaacgt agctgatctg gcagaaaaga
     2401 aaaatgtcct tataattacg gtaaaaacag atgtagaaac cgtttataaa attctctccg
     2461 ttgagaaagt tttgcaggta ttgaaaaaat ttatactcgg gtcctgcaat gcctatcgtc
     2521 ttatggcata ttttatgtct ccggcaatcc gtgggggcgt aatgatcaag gacgcccagt
     2581 gggacaaggg gagtgttgtt gttgtccgga cggggatctg gtttatctcg gctacaaaac
     2641 aagtgtgtgt cccggtaaat gacgttgcgg ccatcgagat taccaaacgg gatgtccagg
     2701 gtaaaccaac agatgtcata agaatcgatc atcttgattc aggtgaagta tcctcttccc
     2761 tggtactctg ccctctttct actctccagg tcctctacaa ttaccttaag gatacgacaa
     2821 agggcatgga tatgaaaggt acggagctcg acagcattga tcagcaggtc gccatgcttg
     2881 tctattccgg catggactcg catgcaatcg agaatatgct caatatccct cataagcagc
     2941 ttgatgcaat ttatgataag atcctcaacc tcggtattgg tgaagtggta acaatccgaa
     3001 gggagattca gctcacgaca aaaggagtac gatatatttc tgacgctaca aaatcccaga
     3061 caaattaggt aaaaaatctt catttctatt ccaatacctc tttaagtaat aatctaccaa
     3121 acaatttatt gattgacgat gggaaaaatt ctgattgtgg atgacactct cttcatgaga
     3181 acactgttaa aaaatatttt gttctctggt ggccatgtaa ttgccggtga ggcaggagat
     3241 ggtgaggagg gaattgccaa atataaggag ttaaagcctg atctcgttac tatggatatt
     3301 gtgatgccca aactgaacgg gattgaagca ttaaaggcca taaaagagtt tgatccggca
     3361 gcaaggattg tcatgtgcac tgccgtagga caggaacaga tggtgaagct cgccatcaag
     3421 agtggtgcga aaggttatat tatcaaacca ttccaggccc cgaaagtttt ggaagaagta
     3481 aaaaacgtcc tggcgtcctg aaggttatgg tgaaagttct cattgtggat gattccgttt
     3541 tcatgcggac gatcataagg gatatgctgg caaaagaatc ttctattgaa atagtgggaa
     3601 ctgcatcaaa tggtctggat gcactggaaa aaattcagga actcaacccg gacctcatca
     3661 ctcttgatat tgagatgccc aaaatgaacg ggctcgaggt tcttttggag ctcaaaaaga
     3721 taaaaaaaca tcccaaggtc ctgatgctga gttctcttac ctctaaagac gctgagatga
     3781 cgcatgaggc gattcgtctt ggtgccgatg attttatgtt aaaacccaaa gatctgcctc
     3841 acatacgcga gattagtgaa gaattgatct caaaggttaa acacctcttt accctcacac
     3901 gatcagaaat tcatcacaaa ccaataccat ctcaagggtc taacgcggac cgggtcgttc
     3961 tcatcgggtc ttcagcaggg ggtccccagc aggtcgatac tctgttatcc atgctgtctc
     4021 caaaccttcc tgccggtgtt attataaccc agcatatgcc cgtcgggttc acggcaccgc
     4081 ttgccgaacg tttcaacaga atagcgacga tgccggtaaa agaaacccag aacggtgatg
     4141 ttgttgaaac aggaaaaatc ctgctttcga aagccgggtt ccacacggtt atttcgggag
     4201 ttttggggga atcaggaaaa acgacaggtc gtatcgtcca tactacctca cccccggttc
     4261 atggtgtcag accagcagtg gataagacct ttgaatccgc tgctcatgta tatggaaaaa
     4321 acgcggtatc ggttatttta agtggtatgg gaaatgatgc aggactggga gcacatgcga
     4381 taaaagaagc gggtggtgtg agtctcatct gcgatgaagc ggattgtctt gtatacggta
     4441 tggcacggtc tgcgataaag aataatgccg ttgatcttat tcttccgtta agaaaactct
     4501 caaatgagat tgaacgtgta gtgtatagta tggagggaac caatgtctga atttgaggca
     4561 tatcgcgagt tattcgtcgc tgaatcacgg gaaaaccatg aaaaccttgt caagaacctc
     4621 ctgctcctgg aactgggctc ttaccaggat gctattgatg agatctttcg ttccatccat
     4681 acacttaaag gggcatccgc ttccatgggt tttgcaaata tggagcatct ctgtcattca
     4741 atggaggatg tcttccagct catccgcagc ggggctgcag aaatttcacc ggatctggtg
     4801 aacctgctcc ttggatgttc agatcttatt gaacagatgc tggatgatat tgaagcgggg
     4861 ggagactcgt cgtcgaaaaa cccggcggag caggtcgaga ccctcaaaaa atggattgag
     4921 caacgaggcg gcacacaaaa agcgcagaat gcggttcctg cagttcctgt tgaggtaaaa
     4981 acatcagaag aacctgaaga ggataaaaaa gcagagaacc gggttgaata tgagatgcaa
     5041 atcaccatct ccagtgaatg ccagatgaaa gatgtgcgtg ccctgctggc tctgggaaat
     5101 ttaaaatccg tgggaacaat tctttccatg aacccgacct ttgagttgat cgatgaggga
     5161 aaatttgatg gaaggctcga actcgtcatc gcaagtgatg ccggggaaga tgcattaaaa
     5221 actgctgctt caggcacaga tatttctgaa attatgatcc gcccggccag gaaaccggaa
     5281 atcacgacag ctggtgattt gcccgagggt ccaaagaagg acgaggcgga gaagaagcag
     5341 tcccagacac ccgataaatc ccgggagata aaaaacctca gggtggatat tcaccagctt
     5401 gaccacatca tgaatcttgt tgaagatctc gtcattaacc ggggaaggct gaaacagatt
     5461 gccgaacacc acaagatcaa ggagatggat gaggcaatcg gcatggttga ccggtcagtt
     5521 tctgaactcc agagcctgat gatgaatatc aggatgattc cgctcaatca tatcttcaac
     5581 cgcctccccc gtgttgtccg tgatgttgcc cagtatgaca acaaagaagt tgattttttc
     5641 atcgaggggg gagaaactga gcttgaccgg agtgtgatgg acggacttaa tgatccgcta
     5701 ctccacctga tcaggaatgc agtaaatcat ggcatagaat cacccgaact ccgggaaaaa
     5761 gccaaaaagc cgcggaaagg atttgtcaag ctttcagcac accgtgaccg ggataatgtt
     5821 atcatagagc ttatcgatga cggggcgggt atcaatattg acaaagtcaa ggcaaaaggg
     5881 attgaaaaag gactgataac tccagaagtt gctgaaacat tcactactga acaggcaatt
     5941 gacctgctat ttcagccagg cttttccact gccgacaaaa tatccgatat cagcgggcgt
     6001 ggagtcgggc tcgatgttgt gagaagatcc atcgaagccc taaaaggtac catccgtgta
     6061 gaaacaacac cgggtaaagg gagccgtttt gagttgttgc tcccgccaac aatggctatt
     6121 gtcgatgtca tgattgtcag gattaatgac aaacgatttg gcatacccat cagcagcatc
     6181 gtcgaagtgg caaattttaa gagggatgaa acacaccaga taggaaaagg tgaagcgatc
     6241 cttctccgtg atgaagtcct gcagatcctc tggcttaatg aaatggttgg agtatcaaaa
     6301 gcctgtgaga tcctgattgt tgtgcaatac cagaagcgca aatgctgtat tccggtcgat
     6361 atcgttgaag gaaagcagga agttgtcgtg aaacctctga gcggttttat tggtaatacc
     6421 cggggtatca gcggagttac cattctcggt gatggagggg tagttccggt tattgatgta
     6481 aacaccataa tataggagaa agaaatgaaa ttatcaacag ttcaggcaga tgcaatgcag
     6541 gagcttggaa atatcggggc ggcgcatgca gcaaccaccc tctcccagat gctgggaagc
     6601 accattgaga tgagcgttcc cgcaatcaag gtaattgacc tgtcccagct gggagactac
     6661 atgggtgaag aatcggcagc aatggttgcc tttgagcttc agggagatat tccccacggg
     6721 ggatatgttt tattttacat aactcgcgaa tccgcaatcc gcatgacaaa taccatgctg
     6781 ggtatgaccg aaaccaaccg cactctgagt gaaatggacg aaagtgccct ccttgaagta
     6841 ggcaatatca tggtctctgc atttcttgac gcaaccgctg aactactcgg ttttgtgatg
     6901 cttccttctc ctccggcact caccatcgat atggcacacg ctgcaatgca gtctctcatt
     6961 gcacagatgc aggaagagac taatgaagta ttgctgttct ccaccgagct atcctgcgag
     7021 gaatacaaga tagacagtga tattattatg atgccggaga gaagcactct tgaaagaatc
     7081 attgcacttc tcgaaaatat gatgaaaacc aactgatgag gcaatgcccg atatgccaga
     7141 ctcccattcc aggaatgaac aaacatcgat gattggtata ggggaatacc gcgtcggttc
     7201 tttccctatg atgactatcg gtctgggttc ctgtatagga ctgacaatgt atgatccaaa
     7261 ccagaaactg ggtgcaatgg tacatatcat gctgccggaa agtgccggta gaaaagatcg
     7321 gccgggaaaa tatgctgaca cagcgattcc actgctggta aatgagctca ccacccttgg
     7381 ctgtaagaac agatctatca tcgcaaagat tattggcggg gcttgcatgt ttgaatattt
     7441 tggcactaac ctcaacattg gtgaaagaaa tgcggaaaaa gtgaggattc tgttaaaaga
     7501 acatcatatt gctctagcag cggaggatgt agggggaaaa gttggtcgat cggtcacatt
     7561 cctgccagca aacgagggga aggtgtccat ccgcagggca gacgggtctt caaccgatct
     7621 ctgaggccgg gttatgtggg ggccggacgt ttcatcctga tttcttcaga gcaatactga
     7681 tcaattgagt ccagaagtgc ttttttagct ctatttacct gaatttcatc atttaagaag
     7741 aaatcgttgg ccaggtattt caggatcgtt gcatagaatt gcagatgagg aaattctttt
     7801 tcaaattccg aaattcgatt taatgcatcc attttttcac cgttaagaag atcaaaagca
     7861 acatcaaaca ggtaaattgt gtggaccggc atgcctttgg tgctggcaag tttcctgaaa
     7921 ctatgatagg ttatcttttt tttcgtgagg agagaaaacc tgagcccgag atagataggt
     7981 tcgagatcat cggggtactg tttaatgagg tggtttaaga tcccggcggc gccaccagcg
     8041 tcccggttat caagatag
//
LOCUS       DASRLV010000069         5741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_260909,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000069 DASRLV010000000
VERSION     DASRLV010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260909"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="VFC43_02840"
     CDS             complement(<1..1078)
                     /locus_tag="VFC43_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZK30158.1"
                     /translation="MMLFKTLRSEIVSHLSYIIGSKGEAFVIDPRRDCEVYSRIAEDH
                     GIRIREIFETHRNEDYVVGSHELSRITGASIHHGPGLPWTYGETLHDGEEFRVGALKI
                     VALHTPGHSPDSTCYVLYDRESGDRPVMVFTGDTLFVGDVGRTDFLGPGKTAMMSGKL
                     YDSLHGKLLTIGDGVVVLPAHGYGSVCGGTIGEREISTIGAERAMNPMLQLTKDEFVT
                     RKVEEKHETPPYFSVMERYNLEGPPIPMHMPASPALKPDAFRAAAAGGIPIIDTRAPP
                     AFGGAHIEGAYSLPPSRLSNAGWVASVEKEVLLVANDPQALDFAVRNLYRMGYSSFSG
                     YLEGGMEEWYKGGNPLSHVDLLTCQ"
     gene            complement(1327..1512)
                     /locus_tag="VFC43_02845"
     CDS             complement(1327..1512)
                     /locus_tag="VFC43_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30159.1"
                     /translation="MREGLMLDFILDEWARARPVLIEVRSREECRMAMQRGRYTFRGM
                     ISETAHRTLDLHHNIIL"
     gene            1774..2190
                     /locus_tag="VFC43_02850"
     CDS             1774..2190
                     /locus_tag="VFC43_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30160.1"
                     /translation="MKLPRKNKDPKVLGNHKRVRDLQHVEDVFPNARACPFPYLKDRP
                     SPGKIIEGKVTRKEIDIKTLYAGQFAVSKKRVREILQEIKDGKYSGHEQYADKIVVVE
                     HEEKNFIWSGHHHATALLLSGAKRVAVVHHIALKEL"
     gene            3247..3882
                     /locus_tag="VFC43_02855"
     CDS             3247..3882
                     /locus_tag="VFC43_02855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HZK30161.1"
                     /translation="MGIIPDGNRRWAVARGLPKSAGYEYGIEPAKRFFKIVWDLGIKE
                     VSTYIFTKENTHRPREQVEAFKKAFITSLDWVEGQDVSLLIVGDTSSTMFPKGIEHFT
                     EPDNDRSERRRLNLLVSYNWKWDVGEVIRTPAQTTSRGDELLSLVGSRHVSNIDLIVR
                     WGGRNRLSGFLPLQSAYADLCVVDAMWPDFEVEQFYDALRWYQDQDITRGG"
     gene            4023..4169
                     /locus_tag="VFC43_02860"
     CDS             4023..4169
                     /locus_tag="VFC43_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30162.1"
                     /translation="MTNQSGSTRRNAMQGNSLSCAMTTYGTGSHRPQDLTAFATLQKA
                     TESA"
     gene            4172..4795
                     /locus_tag="VFC43_02865"
     CDS             4172..4795
                     /locus_tag="VFC43_02865"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZK30163.1"
                     /translation="MLAPGAAAPDISGTNINPKAPDITYSLATLSGKVVVVIFFAWWC
                     PHCVNELGILQNLWKKYQGHEVGFVAVHTESDDAHARQFGYPNALSAATSKLTALGIQ
                     FPAVQDDTVNTIFSHYDGLGYGFPQVCILDKDHVIHSVVSGEEPESATDNRISLLPLE
                     PRAIVILNLYQWGFCGPVSGQSFKTLSHRNLYPEFRDHEAAVKDLFS"
     gene            4872..5387
                     /locus_tag="VFC43_02870"
     CDS             4872..5387
                     /locus_tag="VFC43_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HZK30164.1"
                     /translation="MSRIAVIITDMFQDVEYKQPVDAFRTAGHEIVNVGLRAEVQVKG
                     ENGAVVRIDRAVSDVSVDDFDALFIPGGYSPDKLRIDPDAVRFTREFFAKNKPVFSIC
                     HAPQLLITAQVLKGRRVTGWMSIVQDIKNAGAEYVDAEVVVDKNLVSSRKPADIPAFV
                     REALVVLAKKG"
BASE COUNT         1502 a         1399 c         1415 g         1425 t
ORIGIN      
        1 gctggcacgt cagcagatcc acgtgggaga gcgggtttcc ccccttatac cactcctcca
       61 tgccgccttc gaggtagccg ctgaaggatg agtatcccat ccggtagagg ttcctgacag
      121 cgaagtcaag agcctggggg tcatttgcga cgaggagcac ctctttttca acggaagcga
      181 cccagccggc gtttgacagt ctcgatggcg ggaggctgta ggcgccttcg atatgagccc
      241 ctccgaatgc gggtggggcc cttgtgtcta tgatgggaat accccctgca gcagccgccc
      301 tgaacgcgtc gggtttgagt gcaggggacg cgggcatgtg catcggaatg ggtgggccct
      361 cgaggttgta ccgttccatg acgctgaaat aaggcggcgt ctcgtgcttc tcttcgacct
      421 ttcgcgtaac gaattcgtct ttggtaagct gaagcatagg gttcattgcc ctctcggcgc
      481 cgatggtact gatctcgcgt tcacctatcg tgccgccgca gaccgagcca tacccatgcg
      541 cgggcaggac gacaacaccg tcaccaatgg tgagcagttt cccatgaagg ctgtcgtaga
      601 gtttcccgga catcatcgct gttttgccgg ggccgaggaa atccgtcctg ccgacgtcac
      661 cgacaaagag cgtgtctccg gtaaagacca tcacggggcg gtccccgctc tccctgtcgt
      721 agaggacata gcatgtgctg tccggggaat gccccggggt atgcagggcg acgatcttca
      781 atgccccgac ccggaactcc tcgccgtcgt ggagggtctc cccgtatgtc cagggaagcc
      841 ccggaccatg gtgtatactg gctccggtga tccgggagag ttcatgggag ccgacgacgt
      901 agtcctcgtt ccggtgagtc tcgaagatct cccggatcct gatcccatgg tcctctgcga
      961 tcctgctgta gacctcgcag tccctcctgg ggtcgatgac aaacgcctcg cccttcgagc
     1021 cgattatgta gctcaaatga gaaactatct cagatctcaa agttttaaac agcatcatga
     1081 tagtcccttg tggctctttt tctttaaacg ttgcgagttt ttgcttcctt ctgtgcctgc
     1141 aggaaaatca cgagtggatc cgaggggtca gatggggaag cacatggata aagatacggt
     1201 ccccggtgcc tccctgttca gggtgaccgg gatccatcag gaaccacctg gcccttgatt
     1261 ttggggtggg aaatgcattc gcgggatata ctcccgtcag cctgaccctg cccgctgtcc
     1321 tccctctcac aggataatat tgtggtgcag gtccagagtt cgatgtgccg tttccgagat
     1381 catgccacgg aatgtgtagc gacccctctg catggccatc ctgcactctt cccgagaccg
     1441 gacctcgatt aagaccggcc tcgcccgtgc ccattcgtct aggatgaaat ccagcatcaa
     1501 gccttcacgc aaatcaccga tttctccttc ccgttttttt aaaaaatggc tttgtagaaa
     1561 tcctacattc ttcgaagtaa acaactgacc acaaaagcta aggtacggtg cctccttacc
     1621 ttgttatgcg aataacgagc gagggggcga aaacaaatat aattaaagtg gtattagcat
     1681 gatagataga ttgtgaaggg aatatacaat cactagaaaa tcatagtaac ttaaacgata
     1741 acaatttccc aaaaatattg agggatagat gaaatgaaac tcccacggaa aaataaagat
     1801 cccaaagttt tagggaatca taaacgagtc cgggatctcc aacatgtaga agatgtgttc
     1861 ccgaatgccc gggcttgtcc gttcccatac ctgaaagacc ggccgtctcc gggtaagatc
     1921 attgagggga aggttacacg gaaggagatc gatatcaaga ccttgtatgc aggccaattt
     1981 gcggtatcca agaaaagggt gagagagatc ctacaagaaa ttaaagacgg gaaatatagc
     2041 ggacatgagc aatacgcgga taaaatcgtt gtagttgaac acgaagagaa gaactttatc
     2101 tggtccggtc atcaccacgc cacggctttg ttgctgtcag gtgcaaaaag agttgctgtt
     2161 gttcaccaca tcgcattgaa agaactgtga cttcttctat gagttctctg ataatgtaaa
     2221 ggcactccaa aagcttagtt ttgactttcg ggtcttttta tctaaaaacg tcttcacgtc
     2281 gtatgacctg caatatgcca tcaatctggc agcatcaatc cacattcccg gcaatactgc
     2341 cacgtgattg gatctcccgg catccaaacc acgatattcc tcatgctgaa acactcctta
     2401 aacaatgcca aggatcccac attccgacct ctatatcatg gataagggat ataattccga
     2461 agaaattcat gaattgattc aggataccct caagtcctgt tctctcattc ctgtcagcaa
     2521 tcgaaaacgg aaacgaatat tcgggtatta ctagaagaat cgctcggtca ttccatccga
     2581 tttatcaatt acctgatagt ggagcggacc cggacgaagg ctctgtttga acacgtgaac
     2641 aaattaaaaa aataaactaa ccctcttttt tgatttcaac ccgttttagt ggggaaatcc
     2701 gctatatctg gaaagaagcc catattggtc gcaattcccg agatctcggg aaaggactct
     2761 tttacgggct tctttttcat tctccgatac cctattgata ccctaaactc cttgggttag
     2821 acacccataa agatagggcc gtttgcattg tcggagtatt atatctcaat atgactttgt
     2881 gttgaaacat gattgtcaaa atctagaaca tattaaatta tggagacaaa cattcgacca
     2941 ggaagatccg aaaccataaa ctctgcattc cccatctcgg atcttccaaa actaccttga
     3001 atcccatcaa agtccttttt ataacctatt ctgcaagcct caattccgtt ccaccatatt
     3061 taaacaggga taagcatcta accggaggat catgaattgt cccctatatt agtatttccg
     3121 gaaactttca acgtcaaatg cgaggttctc cgaataatct aaatgtggaa ttaatatgac
     3181 cgcacacatg atgttcactg ccaaagctaa cccgcttcaa aaaatgaaac gtatcccgcg
     3241 tcccatgtgg gaataatccc tgatggaaat cgtcggtggg ctgttgccag ggggcttccc
     3301 aagtccgccg gttatgagta tggtatcgaa cctgcaaaac gtttcttcaa gattgtatgg
     3361 gatctcggta tcaaggaagt atctacgtat attttcacga aggagaacac acacagaccc
     3421 agagagcagg tcgaggcctt caagaaagct ttcatcacct ccctcgactg ggttgaggga
     3481 caggatgtct cgttactcat cgtcggtgat accagttcta ctatgttccc gaagggaata
     3541 gaacatttca ccgaacctga taatgacagg tctgaaagga gacggctcaa tctccttgtt
     3601 agttataact ggaaatggga tgtcggcgag gtgatcagga cccccgccca gactacgagc
     3661 aggggtgacg aattactctc ccttgtcggt tcccggcatg tatctaacat agatctcatc
     3721 gtccgatggg gtgggaggaa ccgcctcagt ggttttctgc ccctgcagtc agcgtacgca
     3781 gatttatgtg tcgtggatgc catgtggcct gatttcgaag tcgaacaatt ttatgacgcg
     3841 ttgcggtggt atcaggacca ggatattacc agagggggtt agatcggttt cattcgctaa
     3901 tccggctaat ttatgaattt tttccagcag tacacaaaag aggcattacg aaaggtgggg
     3961 caaacaatga agaatataat tggggaaagc cagaaaaaat atccggcagg agaaatcaga
     4021 aaatgacaaa ccagtcagga agtacccgaa gaaatgcgat gcagggcaac tcactatcct
     4081 gtgctatgac aacctacggt accgggtcgc accggcccca ggatcttact gcatttgcca
     4141 cgctgcaaaa agccacggag agtgcgtagt catgctcgcc ccgggagctg cagcgcccga
     4201 tatatccggg acaaacatta atcccaaagc cccggatatc acgtattccc tggcgacgct
     4261 gagtggaaag gtagtcgtcg tgatcttttt tgcctggtgg tgccctcatt gcgtcaatga
     4321 actgggaatt ttgcagaacc tctggaagaa ataccaggga catgaggttg ggttcgtcgc
     4381 cgtacacaca gaatccgacg atgcacatgc ccggcaattt ggctatccga atgctctgag
     4441 tgcggccaca tcaaaactga cagcgcttgg catccagttc cccgctgttc aggacgatac
     4501 agttaatact attttcagcc attacgatgg tctgggttac ggtttccccc aggtgtgtat
     4561 ccttgataag gatcacgtca tccattcagt ggttagcggc gaggagcccg aatcagctac
     4621 cgacaatcga atatctcttc tgcctctgga accacgagcc atagtgatcc tgaatttgta
     4681 tcagtggggg ttttgtggcc ctgtatctgg tcagtccttc aaaaccctct cgcatcgaaa
     4741 tctgtaccct gagttccggg atcatgaagc tgcggtaaaa gatctctttt catagatgct
     4801 tttatttccc tgaaaaacat ctggtcagct gatcaggtcc cgggcggacc ggaatcaggt
     4861 agggtactat aatgagcagg atcgcagtga tcatcacaga tatgttccag gatgtggaat
     4921 acaagcagcc cgtggacgct ttccgtacag ctggccacga gatagtgaat gtcggcctca
     4981 gggcggaagt gcaggtaaaa ggggaaaacg gcgctgtcgt aaggattgat agggcagtca
     5041 gtgacgtctc ggtagatgac tttgatgccc tcttcatccc gggagggtac tctcctgaca
     5101 aacttcggat cgatcctgat gcggtccggt ttacccggga gttcttcgca aaaaacaaac
     5161 cggtcttttc tatctgccat gcaccccagc tcctcatcac agcgcaggtg ctgaagggga
     5221 ggcgcgtgac aggatggatg tcaatcgtgc aggatataaa aaatgccggg gcagagtacg
     5281 tggacgcgga ggttgtggtt gataaaaatc tagtctccag ccgaaagccg gctgacatcc
     5341 cagcgtttgt ccgtgaggcg ctcgttgtgc ttgcgaagaa gggctgaggt ttttgattgt
     5401 gcatgaaaga gacgttggaa tcaccacagc gggaaacacg attttccgaa ttttcggttt
     5461 tttttccttg attgggtaac aaaaaatgga atgtatcagt aaaagttaac ccaacaatct
     5521 gttttttatt gtgtcctgct aatactacta ataatactac tagtatgata aaactggaaa
     5581 taaagaacat taaaggccat aactatctct atttcagaga agggctcaaa gttaactcga
     5641 aatcaatatc tgtgctgctt tatggcggca gaatcgataa aatctccgca agagagtttg
     5701 ttgataaaca gagtgagttc acatttctca agttaacaaa a
//
LOCUS       DASRLV010000070         6817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_19436,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000070 DASRLV010000000
VERSION     DASRLV010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19436"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /locus_tag="VFC43_02875"
     CDS             <1..1306
                     /locus_tag="VFC43_02875"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZK30165.1"
                     /translation="GHMKVKTALSAKEALDLLVSTTFDAMIVDYDLPEINGIEFLKII
                     RGKGDTTPIIIFTGVGREYAAIEALNFGADFFLKKGDDVQAQLLEMVQMINQAVDRRN
                     MGKAPGTIQKVLSEAFNFFDQPAFVIDREGKVIVWNKGITGLTGVKPADILGKGDWEY
                     SIPFFGYKTPMLSDLIFQDDAAVLEHKYSIIEREKGTITAWIKATPEEGRQTVLWMQS
                     TALYDSKGVFIGIMGKVRDVTEEMGAELLNKPVQAANEPKPSESPVVSKPGMFDKILG
                     KAKSLHREGLRLSFREGKYLDAIPYFDQALEIDPSLPYVWHDRGVCFREIGRDMEALR
                     NFNRAVELAPNDEELIFSRAEMLKTIGILRHRQDFIELAIQNLNLLVEKNPNHAEAWN
                     CLGICSKEIGKDALSRQYYEKSHELIRQGKNRKKTRNLDLTT"
     gene            1366..2472
                     /locus_tag="VFC43_02880"
     CDS             1366..2472
                     /locus_tag="VFC43_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HZK30166.1"
                     /translation="MKSSVYYASLRAYSDQESTTIKVQKLFDRAGFPGILSPYDKTAV
                     KLHFGERGNDGYISPVYVRQVVDKIKSCGALPFLTDTSTLYIGSRSNAVDHITTAILH
                     GFDYAVTGAPIIIADGLLGKNVVPVKIGKKHFDTVTIAGDIARSDSMIVMSHFKGHIV
                     SGFGGAIKNLAMGCAPPEGKRAQHNARPFTLPDRCSGCGTCVEVCPKKAIALAHEKSA
                     IDRDLCIGCFDCMHVCPEHAIDIDWETDIPVFMERMVEYACGAVRGKEKKTGFMNFLI
                     RITPDCDCFPFSDTPIVPDIGILASLDPVAIDAASFDLVNQQTGFNESLLTAHHHSGE
                     DKFKGVHPQTDGLKQVKYAEEIGMGNRAYDLIKI"
     gene            2716..4305
                     /locus_tag="VFC43_02885"
     CDS             2716..4305
                     /locus_tag="VFC43_02885"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HZK30167.1"
                     /translation="MNLRQPNANEATGTVNRSRDVAPCSGICTRCMDGCKGSCEIWLS
                     SFRGREVLYPGPFGEITAGADKNYPIDYSHLNIQGYAVGAWGLPDGVEAGPDTAVFST
                     VDTETEYGWDKKVKMRLPIFTGALGSTEIARVNWEHFAIGAAISGITCVCGENVCGID
                     PGLKRDSKGKVIESPEMDRRINVYKKFYDGYGEILVQMNVEDTRLGTAEYVASKHNLD
                     TIELKWGQGAKCIGGEIKVKSLERAIELKKRGYIVLPDPTVKENQMAFKAGAIKEFER
                     HSRLGFVTKEGFLEEVDRLRDIGFKRVTLKTGAYSMVELAMALRYCSEAKIDLLTIDG
                     APGGTGMSPWPMMNEWGIPTFYLQSLAYEFACKLEKKGYRVPDLAMAGGFSDEPGVFK
                     ALAMGTPYFKAVCMGRGLMIPGMVGKNIEKWIKEGDLPKTVSKYGNNIEEIFVCYEEL
                     KEKYGARMKDIPMGAVGIYTYAHKFRTGLQQIMAGSRNFRLSTMSRDDLMALTPEAAD
                     VSGIDYVMGARYDEAMGALLD"
     gene            complement(4371..5519)
                     /locus_tag="VFC43_02890"
     CDS             complement(4371..5519)
                     /locus_tag="VFC43_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30168.1"
                     /translation="MIPWLTTNFKNFKPNAAIALNATRHMNKIERKKLFSPDIEPMRT
                     AEGRWFEAIIYEMFLDISKKSSRIKYLARKGADAPRKREEVKLGQNGLFYSKYGDITI
                     RGNGQDLAEFDMLLVDSDNHVAFAEVVTSPSDLKEFEQEIAFKKRLLGYLYGQKTTPF
                     LLISSFDLSNYSVIKRLAKDKTNAIIHTTSCEEIKSVLKHYRPRILYNNPENHPHLIP
                     ATDFPMKHPFDYKRYHDEVRTQIFGEIAKGGKRKLPHITSETGQLVKKVLYGGLFPQS
                     VKLLCDTYGLTVKSKKIEFEDFSKYYSKAIIATDLPNFELIVYLRSRQKKEYHKMVQA
                     KDGHFKFERPTPPKVGFFLWLEAIQPSLGTRITHQLLESFTITQQLGK"
     gene            5690..6046
                     /locus_tag="VFC43_02895"
     CDS             5690..6046
                     /locus_tag="VFC43_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30169.1"
                     /translation="MAKAKKMAGMAPLPQYIMPDESVPEYEAILEQLQDYRKSLIGSS
                     YYYIRAGDDRSCLCRIVHGGGKVFFHELVDHHKHGISDEDIEISVNRDTGSFTLPGYY
                     PISSHIEQKLRILYDI"
     gene            complement(6140..6469)
                     /locus_tag="VFC43_02900"
     CDS             complement(6140..6469)
                     /locus_tag="VFC43_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZK30170.1"
                     /translation="MKMVKAIIKPERFEFVKKALEDKGFIGMTISEVKGRGEQKGIAL
                     QYRGGLMTVDLLPKIQLEIVIRDSDVDNLVVTITEAARTGKIGDGKIFVMPVERSIRI
                     RTGEMET"
     gene            complement(6485..>6817)
                     /locus_tag="VFC43_02905"
     CDS             complement(6485..>6817)
                     /locus_tag="VFC43_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HZK30171.1"
                     /translation="GIICYSAMLFRQKKGLDESLDAWACHGVGGLWGALATGIFAAAS
                     VNGASGLLEGNVHQFIANATGAFAALIYAFVVTYVLALVIDKTIGLRVTEEEEYVGLD
                     ISQHGERC"
BASE COUNT         1861 a         1442 c         1672 g         1842 t
ORIGIN      
        1 cggtcatatg aaggtcaaaa cagcactgag cgcaaaagaa gccctggatc tcctggtgag
       61 caccaccttc gatgcgatga ttgttgatta cgacctgcca gagatcaatg gtatagaatt
      121 tttaaagatt atcagaggaa aaggagatac caccccaatc atcattttta ccggggttgg
      181 cagggaatat gccgcaatcg aggcgttgaa ttttggtgcg gatttcttct tgaaaaaagg
      241 cgatgatgtc caggcccagc ttctcgaaat ggtacaaatg atcaatcagg ctgtcgaccg
      301 gcgaaatatg ggaaaagcac cgggtactat ccagaaggtc ctttcagaag catttaactt
      361 ttttgaccag ccggcatttg tcatcgatcg tgaaggaaaa gtgatcgtat ggaacaaggg
      421 aataacaggg ctaaccggtg ttaaaccggc tgatatactt ggcaagggag actgggagta
      481 ttcgattccc tttttcggct ataagacccc gatgctgagc gatcttatct ttcaggatga
      541 tgcagcagtc cttgaacata aatacagtat tattgaacga gagaaaggaa ccattactgc
      601 atggataaaa gcaacaccgg aggaagggcg tcagactgtc ttatggatgc aatccacggc
      661 actgtatgat agtaaggggg tattcatcgg aattatggga aaagtgaggg acgtgacaga
      721 agagatggga gctgagctcc tcaacaaacc tgtccaggca gcaaatgagc caaaaccttc
      781 agaatctcct gtggtcagta agccgggcat gtttgataaa attctgggaa aagccaaatc
      841 attacaccgg gaagggttgc ggctctcctt cagggaaggt aagtatcttg atgcgattcc
      901 ctattttgat caggctttgg agatcgatcc gtcactaccg tatgtctggc atgaccgggg
      961 tgtatgtttc cgggagatcg gcagggacat ggaagccctc cgtaatttta acagggcagt
     1021 ggagctcgca ccaaatgacg aagaactgat attttcccgt gctgaaatgc taaaaacaat
     1081 tggaattctg cggcaccggc aggatttcat tgaattggca atacagaatc tgaatctgct
     1141 ggttgagaag aaccccaatc atgcagaggc atggaattgc cttggtattt gttcaaaaga
     1201 aattggaaaa gatgcgctgt cccgccagta ttatgaaaag tcccatgaat taatcaggca
     1261 gggaaaaaac aggaaaaaaa cccggaacct tgatttgact acctgaatta agaacgatat
     1321 tgttaaatct cccacaaata tccattttaa ccagaggata ctgccatgaa aagttctgtt
     1381 tattatgcaa gtcttcgcgc ttattcagat caggaaagca ccacaataaa agtgcagaaa
     1441 ctgtttgatc gcgctggttt tccgggtata cttagtccct atgacaaaac tgctgtcaag
     1501 ctccattttg gtgagcgggg gaatgacggg tatatcagtc cggtctatgt gaggcaggtt
     1561 gtggataaga taaaaagctg cggggcgctc cccttcctga ccgataccag tacgctctat
     1621 ataggtagtc ggtcgaatgc agtggatcat atcaccacgg caatccttca cgggtttgac
     1681 tacgcggtaa caggtgcgcc gataattatc gctgacgggc tgttgggtaa gaatgtggta
     1741 ccggtaaaaa tcgggaagaa acattttgat accgttacga tagccgggga tattgcccgt
     1801 tcggacagca tgattgtgat gagtcatttc aaaggtcata ttgtgtcggg attcggaggt
     1861 gcgataaaaa accttgcaat ggggtgtgct ccacccgaag gtaaacgagc ccaacacaat
     1921 gcccgtcctt ttaccctccc ggatcgttgc tccgggtgtg gaacgtgtgt tgaagtctgc
     1981 ccaaaaaagg cgattgcact tgctcatgag aaatctgcta ttgaccgtga tctctgtatc
     2041 gggtgttttg attgtatgca tgtctgccct gaacatgcca tagatattga ttgggaaacc
     2101 gacatcccgg tcttcatgga gcgaatggta gaatatgcct gcggtgcagt aagaggcaaa
     2161 gagaaaaaaa cagggtttat gaattttctt atcaggatca caccagattg tgattgtttt
     2221 cctttcagcg acacaccaat tgttcccgat atcgggatcc ttgcatcatt ggacccggtt
     2281 gcgatagatg cagcaagttt cgatcttgtg aaccagcaga ccggttttaa cgaatctctt
     2341 ctcaccgctc atcatcacag tggagaagac aaatttaagg gggttcatcc acaaactgac
     2401 gggttgaagc aagtgaaata tgcagaagag atcggaatgg gtaatcgcgc ttatgatctg
     2461 ataaaaatct gagatttctt tttctttatt ttgtaaattt ttcccatatt cttgaatttt
     2521 tagcgctcat tttatgaaaa atcggtggaa atgtattgcc atcgcaagaa aaaatagaaa
     2581 tgtttaaaag agggtggaaa gcaagcgctt attagtgaat gcgttttaaa tcaattagtt
     2641 tgagatcgat ctttcgctca aaccgtatct tcgtcattat cgaaggttat ccagataatt
     2701 caatggagtg tattcatgaa tcttagacag ccaaatgcaa acgaagcaac gggaacagtc
     2761 aaccgttcac gggatgttgc tccctgttcc ggtatctgta cccgctgtat ggacggatgc
     2821 aaaggcagct gtgagatctg gttatcatcg ttcaggggca gggaagttct ttatcccggt
     2881 cctttcggtg agatcactgc aggtgccgac aagaactatc cgattgatta ttcccacctt
     2941 aatatccagg gatacgcagt cggtgcatgg ggacttcctg atggcgttga agccggtccg
     3001 gatacagcgg tcttctcaac cgtggatacc gagaccgagt acgggtggga taaaaaagtt
     3061 aagatgcgct taccaatctt cacaggtgcc cttggatcga cggaaattgc tcgggtaaac
     3121 tgggaacatt ttgctatcgg tgcagcgata tcgggtatca cctgtgtctg cggtgaaaac
     3181 gtctgtggta tagatcccgg gctgaagcgc gattcaaagg gcaaagtgat cgagtctccc
     3241 gaaatggatc ggaggattaa cgtctataaa aaattttatg atggctatgg ggaaatactc
     3301 gtccagatga atgtggaaga cacccggctt ggaaccgcgg agtatgtcgc aagcaaacac
     3361 aatttggata ctatagaact caagtggggc cagggtgcca agtgtatcgg tggcgagatt
     3421 aaagtcaagt ctcttgagcg tgccatcgag ctcaagaaac gcggctatat cgtactaccc
     3481 gacccaacgg taaaagagaa ccagatggct ttcaaggcag gtgcaatcaa ggaatttgaa
     3541 cgccactcaa ggctcggttt tgttacaaaa gagggattct tagaagaggt tgaccgtctc
     3601 cgcgacatcg gtttcaaacg cgtgacgtta aagaccggtg cttattccat ggtcgaactg
     3661 gcaatggcct tacggtactg ttccgaggca aaaattgatc tgctcactat tgatggtgca
     3721 cccggcggaa ccggcatgag cccgtggccg atgatgaacg aatggggtat tcccacgttc
     3781 tacctgcagt cacttgcgta cgaatttgcc tgcaaactgg aaaagaaggg ataccgcgtt
     3841 cctgatcttg cgatggctgg cggtttctcc gatgaaccag gcgtattcaa ggcactggct
     3901 atgggaaccc cgtatttcaa ggcagtctgc atgggtcgag ggctcatgat ccccggtatg
     3961 gtaggaaaaa atattgagaa gtggattaaa gaaggtgacc ttcccaagac ggtttcgaag
     4021 tatggaaaca atattgagga gatttttgtc tgttatgaag agctcaagga gaaatatgga
     4081 gcccgcatga aagatatccc gatgggtgca gtcggtattt atacctacgc gcacaagttc
     4141 aggaccggtc tccagcagat tatggcgggc agcagaaact tccgtctatc gacgatgagc
     4201 cgcgatgacc tcatggccct cactcctgaa gcagcagatg tttccggtat cgattatgtt
     4261 atgggtgccc ggtacgatga ggcaatgggt gccctcctcg attgaacata taaaaaaaca
     4321 atcccctttt tcggggtatt tccctgaaaa ttttttttaa caaaaccgtt ttatttccca
     4381 agctgttgtg ttatggtaaa tgattcgagc agctggtgag taatccgcgt tcccagtgag
     4441 ggctggatag cttcaagcca gaggaaaaat cctacttttg gaggggttgg acgttcaaat
     4501 ttgaaatgcc cgtcttttgc ctggaccatc ttgtgatatt cctttttttg tcgcgatctg
     4561 agatagacga tcaattcaaa atttggaaga tcggtcgcaa tgatcgcctt tgaataatat
     4621 tttgaaaaat cctcaaactc tatttttttg ctttttaccg taagaccata ggtatcacag
     4681 agcagtttga cagattgggg gaacaaaccc ccatagagaa ctttttttac aagttgtcct
     4741 gtctcgctgg taatgtgagg gagttttcgt ttacccccct ttgcaatctc gccaaagatc
     4801 tgggtacgca cttcatcatg gtaccgctta taatcaaacg ggtgtttcat agggaaatct
     4861 gtagccggaa tcagatgagg gtggttttcc gggttgttgt agagaatacg ggggcgataa
     4921 tgcttgagaa ctgatttgat ttcttcgcag gaagtggtgt gaatgattgc attggtctta
     4981 tccttcgcca gcctcttgat cacagagtag tttgaaaggt caaaagacga aataagtaaa
     5041 aaaggggtgg ttttctgtcc gtacaggtac ccgagaagtc gtttcttgaa cgctatctcc
     5101 tgttcaaact cttttaaatc tgaaggagag gtcactactt cggcaaatgc cacgtggtta
     5161 tctgaatcga caagcagcat atcgaattct gcaaggtcct gtccgttacc ccggatggta
     5221 atgtccccgt atttcgagta aaaaagaccg ttctgtccga gttttacctc ttcccgtttc
     5281 cggggggcat ctgcaccttt ccgcgccagg tattttatcc ggcttgattt ctttgagata
     5341 tccagaaaca tctcatagat aatggcctcg aaccacctgc cttctgcagt ccgcatcggt
     5401 tcgatatccg gggaaaaaag ttttttccgt tcaatcttat tcatatggcg cgtggcattg
     5461 agggcaatgg ctgcattcgg tttaaaattt ttaaaatttg tcgtaagcca cgggatcatg
     5521 attgcagtat agaatttcag ccatcatcgt aaataacaat ctgctttctt gttcgttttg
     5581 ttttttgaat actaacacaa attataaata gcttagaaag atattgcctt cgtaccttag
     5641 ctatgggtaa acatagcaag ggagagacaa ccaaaggggg atgtaagaaa tggcaaaagc
     5701 aaaaaaaatg gccggcatgg ctccattgcc acaatatatt atgccggacg agtcggtacc
     5761 cgaatacgag gcgatcctcg agcaacttca ggactatcgc aaatcgttga tcggctcttc
     5821 ttattattat ataagggcgg gtgatgatcg ctcctgcctg tgcaggattg tacatggcgg
     5881 ggggaaggta tttttccatg aactggtgga tcaccataaa catgggataa gcgatgagga
     5941 tatcgaaata tcggtaaata gggataccgg atcgtttaca ctgcctgggt attatcctat
     6001 ttcatcgcat attgaacaga agctccggat tttatacgat atataatcta ttttttcgtt
     6061 gcatcgaacc cattatcaga agaaattcta tagggggaaa attgaaaaaa taaaaagagg
     6121 tgtccggtgt tttaattact caggtttcca tctctccggt tcgtatcctg attgatcttt
     6181 ccacaggcat aacgaatatt ttgccatccc caatctttcc cgttcgggct gcttcagtaa
     6241 tcgttacaac aaggttatcg acatctgaat ccctgatgac aatttccagc tgaattttag
     6301 ggaggagatc gactgtcatc aatcccccgc ggtactggag ggcaataccc ttttgttcac
     6361 ccctgccttt cacttcagaa attgtcattc cgataaatcc tttgtcttca agtgcttttt
     6421 ttacaaattc gaaccgttca ggtttgataa ttgcttttac cattttcatg attactcctc
     6481 cagatcagca ccgttctcca tgctgggaga tgtcaagtcc gacatactct tcctcttcag
     6541 tgacacgcag accgattgtt ttgtcaatta ccagtgcaag gacgtaggtt accacaaacg
     6601 catagatcag ggctgcaaag gcaccggttg cgttcgcaat gaactggtgg acgttgcctt
     6661 ccagaagtcc tgatgctcca ttgaccgaag ctgccgcaaa gatacctgtt gcgagagctc
     6721 cccacaggcc acctactcca tggcatgccc atgcatcaag actttcatcc agaccttttt
     6781 tctgtcggaa cagcattgcg ctgtaacaga ttatccc
//
LOCUS       DASRLV010000071         4959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_585977,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000071 DASRLV010000000
VERSION     DASRLV010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585977"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VFC43_02910"
     CDS             <1..338
                     /locus_tag="VFC43_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30172.1"
                     /translation="GEQTIKIDTYSPVSWFEVTVRNKTSGEIYLRDGFGTGKGYTEYL
                     NRTLKVLKRDDMQIEFAGNNITATAMVWVKPESNIDDTSKFNLSTDCAYIDPNPRDFL
                     VVSTTKPST"
     gene            495..566
                     /locus_tag="VFC43_02915"
     tRNA            495..566
                     /locus_tag="VFC43_02915"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:528..530,aa:Arg,seq:gcg)
     gene            complement(1301..2338)
                     /locus_tag="VFC43_02920"
     CDS             complement(1301..2338)
                     /locus_tag="VFC43_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HZK30173.1"
                     /translation="MVKELLKKLSNAHGVSGSESSVFALIKKELKDHVDEIHEDPLGN
                     LIAIKRGNKFKVMLAAHMDEIGLMVKYVDEKGFIRFVALGGWYGPTLYNQRVILHGSK
                     GKYLGVIGGKPPHMMDDDERKKGVKIDDMFIDIGAKNREEVDYLGIEVGTPVTIDREF
                     VELAYNRVSGKAFDNRAGVAMLIKALKEMDSPLTVFGVFTVQEEVGLKGARTSAYTLD
                     PDCAIATDVTIPGDHPGIEMKDAPVEMGKGPVITLVDSSGRGLIASRKVVKWLKDSAD
                     SHGIPVQLEVGSGGTTDATAIHLTKGGIPSTTVSIPSRYIHSPVEVLDTEDIEAGVRL
                     LVAALKNKPVL"
     gene            complement(2340..3440)
                     /locus_tag="VFC43_02925"
     CDS             complement(2340..3440)
                     /locus_tag="VFC43_02925"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZK30174.1"
                     /translation="MKFAHRVQGIEISGIRKIFEAAGPDSINLGLGQPDFDTPQHIKD
                     AAIKAIQEGKTGYTNNTGIPELRLAIGKKLKNENGLTCEPEEIIVTAGASEALHIVMQ
                     ALVDQGDRVLCPDPGFVSYASLATLAGGNPVSMPLTKTLHIDVEKAKALMDGAKIIVL
                     NSPANPTGAVENKESIQALVEYASDAGVTVVSDEVYEHFIYGKQHWSAARFGENVVTI
                     NATSKTYAMTGWRLGYLAAPEEIVGQCIKVHQYCQACATSISQYAALAAYTGDQSPVK
                     MMRDEYQVRRDLFCKGLEDLGFLFPVPEGAFYTFVPMKPALTQKIIDNGIIIVPGAAF
                     GVNAPEYARFSYATSRENLYRALGRIQKIMRD"
     gene            complement(3458..4057)
                     /gene="hxlB"
                     /locus_tag="VFC43_02930"
     CDS             complement(3458..4057)
                     /gene="hxlB"
                     /locus_tag="VFC43_02930"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HZK30175.1"
                     /translation="MERHRVQDMMRLMAQKIRAIANIISDGDVEKFINELLHAKRIYV
                     MGAGRSGLVAKAFAMRLMHLGFQAFVVGETITPSLTKGDIMVIFSGSGRTKTVADIAE
                     TAKEIGAKICLITSDAHSRIGKISDCIVIIEHHRDTVTDDAVEFEIRQMMGDHKSFAP
                     LGTLFETASMIFADAVISRLMEITHVDEIALKNRHTNVE"
     gene            complement(4213..>4959)
                     /locus_tag="VFC43_02935"
     CDS             complement(4213..>4959)
                     /locus_tag="VFC43_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZK30176.1"
                     /translation="GYGIYHDDVIKMLSGCCLVDKHEISRIIVTHADADHCGAAGYFT
                     APVLLHEGTLAIIQKNNRAYGSRRENSVLEEFYTKMINLFSKFNSQSRIECLSKKEDR
                     MRGIFPVMGTVRVGDLDLEILEGLGGHTWGQIYLYSEKHGLLFAADSVINFSSLTKER
                     ADYSSLAAFLVTSVNVDSDLAKKERRALLEIAYQTDNELAAKGKRCLICGGHGAVSVL
                     ENDKLIVNGSIERYQPHQTCTIGGGGKELG"
BASE COUNT         1274 a         1185 c         1037 g         1463 t
ORIGIN      
        1 gtggtgaaca gaccatcaag attgatacct acagtcctgt gtcatggttt gaagttactg
       61 ttaggaataa aaccagcggt gagatttatc ttcgggatgg atttgggaca gggaaggggt
      121 acaccgagta cctgaaccgg actctcaaag tattgaaacg cgatgatatg cagattgaat
      181 tcgccggtaa taatattacc gcaacggcta tggtttgggt taaaccggaa tcaaatatcg
      241 atgatacttc aaaattcaac ctgtccacag attgcgcata cattgatccc aatccccggg
      301 attttttagt agtatccaca acaaaacctt caacgtgatt caaataatct gatctatccc
      361 aaagacatcc ttttttagtt ttgaatcggt tactaactgg tttctgcctt gcatatttca
      421 ctctctggcc ataaacggtg aggtatttat gtgatgaaag cgattttagt aaggcagaca
      481 gacctaaatt ccgtgtcccg gtggggtagt ggacatccta gaagattgcg gatcttttga
      541 cccgggttca agtcccggcc ggggcgtctt cttttcagtt caaaatcttg tatccatcat
      601 tcaaattctg ttctgaatta taaactaata atataagtcc ggcatgggcc ctctacattc
      661 agcgtccaaa tctcttctta atcattcaac gtgtcaaatt tcgataaccg cgattcagtt
      721 aaaattttct ggagcaatat atcctgtaat atgaagtatt cttgacatgg cagttcttcc
      781 tggtccaaaa tgattctgtc atggagatcc agatcgatgg cacgtaccgt atttctggtg
      841 gctactgaac gattctatag aaaaatatta ttggatctac aagcattgtc gaactgagat
      901 ctaacctgat tgtacattta tcgttataat ttttgtaatt gagggaagat gataaaaaga
      961 gggagaaccg tggatggttg gtgtatcatt acaacacata aaggagaaca agtatggcac
     1021 aaaatgtaca aaaactggca cgtataattg ctgtagttat tggagtgatt gtgattatat
     1081 tatcattgat acgatacttc cctctgtcta atgaaggact tgtattagca gcttttttga
     1141 catgctgggg acttgatgcg ttaatttaaa tcaaaatttc tctctgattt acttcaattt
     1201 gccttggcgg ggagtgtttg ttgtatagtt tatttttttt tagtatagag tgttccacca
     1261 ttaaaataat tattcatttt ttaaaaaaga gaaaatgatg ctataacact ggtttgtttt
     1321 tcaatgctgc gacaagcagt cttacaccgg cttcgatatc ctcagtgtcc aggacttcaa
     1381 caggagaatg gatataccgt gaagggatgc tgaccgtagt gctggggatt ccacccttgg
     1441 tcaggtgaat agcagtcgca tccgttgttc ccccgcttcc gacctccagc tgtaccggaa
     1501 ttccatggga gtcagctgaa tccttgagcc atttcaccac tttccggctt gcaatgagcc
     1561 cgcggccgct gctgtccacg agagtaatga ccggtccctt ccccatctcc accggtgcat
     1621 ctttcatctc gatccccggg tggtcaccgg gtatcgtaac atcagtggct attgcacagt
     1681 caggatccag cgtataggca cttgtccgtg ctcctttgag tccaacttct tcctgaactg
     1741 taaaaacacc aaaaacagtc agtggggagt ccatctcttt taaggctttt atgagcatag
     1801 caactcctgc acgattatca aaagccttgc cggatacccg gttataggcg agctcgacaa
     1861 actccctgtc aatcgtcacc ggcgttccca cttcaatacc gaggtaatcg acttcttccc
     1921 ggttctttgc cccgatatca ataaacatat cgtcgatttt cacgcctttc ttacgctcat
     1981 catcatccat catgtgaggc ggtttgcccc cgatgacacc aaggtacttt ccctttgacc
     2041 catgcaggat cacccgctgg ttatagagcg tcggaccata ccagccacca agggcaacaa
     2101 accggataaa acctttctcg tcaacatatt tgaccatcag cccgatctca tccatgtgtg
     2161 cagcgagcat cactttgaat ttgttccccc gtttgatggc gatcaggttg ccaagcgggt
     2221 cttcatggat ttcatcaaca tgatctttga gctctttttt tatcagagca aatacactgc
     2281 tttcactacc ggacacacca tgtgcgttgg ataatttttt caataattct ttgaccatat
     2341 caatccctca ttattttctg aatccttcca agggcccggt acaggttttc ccgcgaggtg
     2401 gcatagctga accgggcata ctccggtgca ttcacaccaa acgctgctcc cggcacgata
     2461 attatcccat tatctataat tttctgggtg agtgctggtt tcataggtac aaaagtatag
     2521 aatgcaccct cggggaccgg gaataaaaaa cccaggtcct caagcccttt gcagaaaaga
     2581 tcccgtctga cctggtattc atcccgcatc atttttaccg gactctggtc cccggtatag
     2641 gctgcaaggg cagcatactg ggaaattgag gttgcacatg cctggcagta ctggtgtact
     2701 tttatgcatt gtccgacgat ttcctcagga gcagcaagat atcccagtcg ccagccggtc
     2761 atcgcatagg tcttgcttgt tgcgttaatc gttactacgt tctcgccaaa acgggcagcg
     2821 ctccagtgct gtttgccata gatgaaatgt tcgtatactt catcggaaac aactgttact
     2881 cctgcatcac tggcatattc tacaagtgcc tgaatggatt ctttgttttc aaccgcacct
     2941 gttggattgg ccggggagtt taaaacaata attttagcac cgtccatcaa tgcctttgcc
     3001 ttctccacat cgatatggag ggtttttgta agcggcatgc ttacagggtt tccgcctgcc
     3061 agagtggcaa gggatgcgta ggaaacaaaa cccgggtcag ggcagagaac ccggtccccc
     3121 tgatcaacca gtgcctgcat tacaatgtgg agtgcttcac ttgcaccggc tgtaacaatg
     3181 atctcttcgg gttcgcaggt taatccattt tcatttttta attttttacc aatagccaaa
     3241 cggagttcgg gaattcccgt attattggta taaccggtct ttccctcctg aattgctttt
     3301 atcgctgcat ccttgatatg ttgtggtgta tcaaaatccg gttgacccag tcccaggttt
     3361 attgaatccg gacccgctgc ttcaaatatc tttctgattc ctgatatttc gataccttgt
     3421 acccgatgag caaatttcat cctcctctcc tccacactta ttcaacattc gtatggcgat
     3481 tcttcagtgc tatttcatct acgtgggtga tttccatcag ccgggaaatt accgcatcgg
     3541 caaagatcat ggacgcggtc tcaaagagtg tccccaaggg ggcaaacgat ttatgatctc
     3601 ccatcatctg ccttatttca aattcaactg catcatctgt caccgtatca cggtggtgct
     3661 caataatcac aatgcaatct gaaattttcc caatcctcga atgtgcatca gaggtgatca
     3721 ggcagatttt tgccccgatt tcttttgctg tttctgcaat atccgcgaca gtcttagttc
     3781 tgccggaacc tgaaaaaatg accatgatat cccctttggt caacgagggg gtaattgtct
     3841 caccaacaac aaatgcctgg aatccgagat gcataagacg catggcaaaa gcctttgcaa
     3901 ccaggccgga acgacccgct cccataacgt agatacgttt tgcatggagc agttcattga
     3961 taaatttttc tacatctcca tcggatatga tattggcgat tgcacgaatc ttttgtgcca
     4021 tcagccgcat catatcctgt actctgtgtc tttccatatt tcctgcacaa aggatgcatc
     4081 cacatagtag atgagaattt cgtagttgtt ttttttgcaa tcaaaatggg ttttttcttt
     4141 tgagaaaagg gaactatgca tctgtgaaaa atacagtttt tactacaaga atccattcct
     4201 gcatgtagcc agttatccga gctctttccc gccaccaccg atagtgcagg tttggtgtgg
     4261 ttgatatcgc tcgatactac cattcacaat gagtttgtca ttttcaagaa ccgacacagc
     4321 accatgaccc ccgcaaataa ggcatcgctt cccttttgcc gcaagttcat tgtctgtctg
     4381 gtaagcgatc tccaggaggg ccctgcgttc cttttttgcg agatcactat cgacattaac
     4441 tgacgtaaca agaaaggctg cgagtgaact ataatcagca cgttcttttg tcagacttga
     4501 aaagtttatc acactgtctg cagcgaagag caatccgtgt ttttcagaat acaggtagat
     4561 ttgaccccac gtgtgacccc cgagcccttc aaggatttcc aggtcgagat ccccgaccct
     4621 gaccgttccc attacaggaa aaattccccg catcctgtcc tctttttttg acaagcattc
     4681 tatcctagac tgggaattaa atttcgagaa aagattaatc attttggtat agaactcttc
     4741 aagaacagag ttttcccttc gggatccgta cgcccggttg ttcttctgta tgattgcaag
     4801 agtaccttca tgcagaagaa ccggtgcggt gaaatacccg gcagccccgc aatgatcagc
     4861 gtctgcatgg gtaacaatga ttcgagaaat ctcgtgttta tccacaagac agcatccgga
     4921 aagcattttt atgacgtcat catgataaat gccgtaccc
//
LOCUS       DASRLV010000072        10494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_20319,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000072 DASRLV010000000
VERSION     DASRLV010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 10494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10494
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20319"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VFC43_02940"
     CDS             <1..319
                     /locus_tag="VFC43_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30177.1"
                     /translation="FPGKMIILGKSVNTASSRPDNPQMPVTDQFCVLSEEGKLFELMY
                     SFDGFLTDSYLNPIDAKTAIEHYGYDLMYMLYCAIHDYLKGEEALIEALEKVITFVFA
                     RKP"
     gene            complement(563..636)
                     /locus_tag="VFC43_02945"
     tRNA            complement(563..636)
                     /locus_tag="VFC43_02945"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(600..602),aa:Ile,seq:gat)
     gene            775..2544
                     /gene="iorA"
                     /locus_tag="VFC43_02950"
     CDS             775..2544
                     /gene="iorA"
                     /locus_tag="VFC43_02950"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HZK30178.1"
                     /translation="MGRKYILGNEAIAHACLESGVNFISGYPGTPSSEVIDTLRAQPY
                     PSYYLEWSVNEKVALENALAAAWCGIRALCTMKHVGLNVAADPLMTSAYTGVTGGLVI
                     LSADDPFAHSSQNEQDSRCYAHFARVPCLDPATLQEAHDMIPEAFRISEEFGIPVIFR
                     PTTRICHSKGDVELKEAVAVHRDGEFHKNPRNYVVIPAHTRVLHKMLNEKQSAIRKRL
                     VERGFNNYQIRGKIAIIASGIGASYVQEILPNGVSFLKIGAYPIDEEWLGDFVRKHER
                     VLVIEELAPEVEEIVRQVAGNIPVFGKKNGFAPYEGELSPPLVADIMAKAGFIKQDPY
                     PLNDPIQNLPPRPPILCAGCLHRSAFYAIKKVFKDAIYPSDIGCYTLGLQLGVVDTTI
                     CMGASITVASGMSHAGDPRDIVCTIGDSTFLHTGIQGLINAVYNGANITVVILDNRIT
                     AMTGHQPNPNTGITACGVESPPVSLDAICRACGVDFVENVDPYDITGMVNILQEVKKR
                     KGVKVVIAKQMCVISARRAGVKRGRYTVNADLCTGCGTCVRFGCPAIEFADEHARIND
                     LCSGCSVCVQLCPTGAIVREAKK"
     gene            2541..3125
                     /locus_tag="VFC43_02955"
     CDS             2541..3125
                     /locus_tag="VFC43_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HZK30179.1"
                     /translation="MSRSFDILIVGIGGQGTILASNILGEACLLEGRHVKGAETHGMA
                     QRGGSVESHIRIDGLFGPLIPPGYADLLISFDLLEALRYSHYLKKEGKMVVNRHLVLP
                     TSVYTQKLVVPGEEEIIAALKKHVLCLIDADKIATEAGSPLSQNVVMLGAASQSMPLK
                     PESLLMAVRNLVPKKTIDINVKSFEMGREFGGTC"
     gene            3125..3853
                     /locus_tag="VFC43_02960"
     CDS             3125..3853
                     /locus_tag="VFC43_02960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HZK30180.1"
                     /translation="MKRQLGAFDPDTIYQGNALQLMPLVSDGIIDLIITDPPFAIDFK
                     ARRENYNRTGSNVLEGYKEIPPAEYREFTQKWIAEAARVLAPDGSMYVFSGWNRLKDI
                     LEGLDAAGFTTINHIIWKYQFGVFTKKKFVTSHYHVLFVVKDPKRYTFNKVDHYAEDV
                     WVINREYWKGKIKTPTKLPSELIKKILLYSSNHGDLVFDPFLGSGTVAIVSQAEGRHF
                     LGFEIVPEYFAFANECLRSQRKRM"
     gene            complement(3868..3951)
                     /locus_tag="VFC43_02965"
     tRNA            complement(3868..3951)
                     /locus_tag="VFC43_02965"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3917..3919),aa:Ser,seq:gga)
     gene            complement(3964..4959)
                     /locus_tag="VFC43_02970"
     CDS             complement(3964..4959)
                     /locus_tag="VFC43_02970"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided pseudouridylation complex
                     pseudouridine synthase subunit Cbf5"
                     /protein_id="HZK30181.1"
                     /translation="MTGDPDTRELLHLWNHAGIMVVDKPRGPSSHQVAAWVGKMLGCQ
                     VGHAGTLDPQVSGVLLIMLGNAVRLAPLLLQHDKEYICVMRLHGDVDEIRMEQIGAEF
                     TGRLYQRPPRKSAVKRVLRIRTIQKLEILGKSGRLVIFRVQCDAGTYIRSLCHHIGLA
                     LGVGAHMVELRRTRSGSFSEQEMHTLHDVQDACSSAKKGNLTELMSMILSVDAAVPDL
                     PTVIIRDTAIDAICHGATLAGIGVIKCDDFKKDQVVAVLSQKKEFVCLGKAIVTSSSF
                     KPGDTGLIIRPTTVFMQPGTYTRGWTKSVKAKDPDEKPKKIPKKEIPLKKYPSNR"
     gene            complement(4961..5650)
                     /locus_tag="VFC43_02975"
     CDS             complement(4961..5650)
                     /locus_tag="VFC43_02975"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HZK30182.1"
                     /translation="MKIAVTRLTGKEKNDAARCAQFGHICYSVHPLHSEIRSHAIKLF
                     IEAVERGEFDCLFFTSALPAKIIAPLLNRYPRIIAIGPQTAKELLHFNIDCETLTSFY
                     SRDFVPYLGDWVRGKHIGIPRADVPNHSLIDAISAAGGIPHEFKCYSLQPTGESLNLD
                     EADAVLFTSTMSYTKAVWKKNPDLIVMAIGDITAAKMRESGTDPIVIGDGSLEGSLTA
                     LNEYLTRQGSG"
     gene            5729..6943
                     /locus_tag="VFC43_02980"
     CDS             5729..6943
                     /locus_tag="VFC43_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HZK30183.1"
                     /translation="MIEILLGVFIGVVLGTISGIIPGVHANTLAGVLLSLQVVLLSFF
                     GPLVLAGAMFAALITHTFVDSVPSTFLGIPDADTSVAVLPAHALCLEGKGEEAVRIAA
                     LGSAGAMIIAIPLSVICFFLLPAFQPYFDWWIGILLVATMGYMIVTSESPGWALTLFC
                     ITGMLGFFTLNYTFLSWHTLAGNSAILMPLLTGLFGISVLLTASKGTMPEQHYQGISM
                     EDRTIMKYSAIGTIAGVAVGWLPGLSTASANSVLASLIGYEKNSRTYILATSAANTSN
                     AFIGLAALFALSRMRNGVMVALSELPLPTMSELTVVGVLAACAAYVITVMLSGSAGRL
                     NGIDSRSMNRAVIAFIVILCILLTGPFGVIILILATALGLIPQLVNVPRVFCMGAIIV
                     PVIFYSFGFAWI"
     gene            7018..7881
                     /locus_tag="VFC43_02985"
     CDS             7018..7881
                     /locus_tag="VFC43_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005730 - nucleolus [Evidence IEA];
                     GO_function: GO:0030515 - snoRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NOP5/NOP56 family protein"
                     /protein_id="HZK30184.1"
                     /translation="MQSYWFGDVNERCCTLFMGDLNAIIDRVQSISTSQDNIIPLDWQ
                     QAILCGACHDRTDYLAKLQEICITAAERSIREQYSRKDAELLQMVRTLDEFDKVINLL
                     SERAAEWYTVRHPAFSRKYRRTPAHLLVKTMREKNRGALGSVAGEIERLTETRTILAK
                     EVSARANNVMPNTSALIGGLVAARLLSHAGGLLLLSRLPASAIQVLGARTALFSHLKT
                     RSPCPKHGIIFQHRRVHNAPRSVRGKVSRVLAGKLAIAARLDYFRGVLVPDFIDAAQE
                     RIDSTGKRGES"
     gene            7878..8486
                     /locus_tag="VFC43_02990"
     CDS             7878..8486
                     /locus_tag="VFC43_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrillarin-like rRNA/tRNA
                     2'-O-methyltransferase"
                     /protein_id="HZK30185.1"
                     /translation="MIWIGDVLVSHGAGGVYNERLLNGARVWDPNRSKLSALYHIGTG
                     IELTSGMRVLYLGAANGTTVSHVADYVEVVYAVEFAPRPMQDLLEVCRRRRNIIPIMA
                     DASRPECYVPLVESVDLIYQDVAQPDQASIALRNCLFLKEGGFLILMLKTRSVNVRKE
                     PAEVFQDTLTSLTSGGLIIQESVWLTPYHHDHAAIICKKPGV"
     gene            8496..9212
                     /locus_tag="VFC43_02995"
     CDS             8496..9212
                     /locus_tag="VFC43_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HZK30186.1"
                     /translation="MSDKVRFFSAGPISILTIVLLVGLAIILIPLLILGIIGAAFTKL
                     GFSWISALAVVFCVVFGSFVNIPLYHIQRDVVQAYRNETSDFDVCVPCASTPVWDTLV
                     SINLGGAVIPTCVSLYMLYGTIRIMGAPLIFTVFVAIALVGIIAFISTRLVTYSGIQV
                     PLLIPGLTALLAGLVLTGGTGPTAAVTAFVSGTTGVLIGGNIANFYRIKNLEVKAVSF
                     GGAGTFGSIFICCMLPALIA"
     gene            complement(9278..9350)
                     /locus_tag="VFC43_03000"
     tRNA            complement(9278..9350)
                     /locus_tag="VFC43_03000"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9314..9316),aa:Gln,seq:ctg)
     gene            complement(9407..10132)
                     /locus_tag="VFC43_03005"
     CDS             complement(9407..10132)
                     /locus_tag="VFC43_03005"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HZK30187.1"
                     /translation="MRIIRAPSIGRAHELVIKMILEKGWILQTEDDEATIEFEEVTLQ
                     VDTPLAEPAVSPDSRFQKRFVEQYARDLLHGSHASFEYDYHGRLFDWGERLVADGKPV
                     HIDQISYIVEKIKSSPSTRRAIAITWNPVIDQMLDNCPCLQLVQCVLRDRKLNMRVIF
                     RSNDMLTAAGANMYALVQLQKSIADSLGASCGSYTHISLIPHIYYIRDINDIEPFCKK
                     GEIIQPAREVCHACRKCERAKEL"
BASE COUNT         2679 a         2388 c         2525 g         2902 t
ORIGIN      
        1 tttcccggga aagatgatca ttttagggaa atctgtaaat acggcatcgt ccagacccga
       61 caatccccaa atgccggtca ctgaccagtt ctgtgtatta tctgaagaag gtaaattatt
      121 tgaacttatg tacagtttcg acggcttcct taccgattcg tatcttaatc cgatagatgc
      181 aaagaccgca atcgagcact atggttatga ccttatgtac atgctttatt gtgcgattca
      241 cgattatctg aaaggagaag aggcactgat tgaagcactc gaaaaagtga ttacctttgt
      301 ttttgcacgt aaaccgtgac tggaatgatc agtcaacttt ttttaatatt tatctcgtag
      361 tttatgattg atttttgatt tatcgtacat gtagtaaatt ttctatcagc aagattctac
      421 cgcctggcaa tgggaaaaaa ccccttgcct ctatcgaaaa aacaatcaac aaaatctgaa
      481 atgctggtaa tattgaaaag actagtaaaa tgatttttct cgaagatctc atgaaggaaa
      541 aacaataatt gcaataaaag gatgggcccg atgtgattcg aacacatgac ctcccggtta
      601 tcagccgggc gcaccaccgg gctatgctac gggcccataa gtttgcctga aatttacctt
      661 atttactact ccatcagagt ttttataagt tgtgaattgc agagtggaga aggcagaaca
      721 atcagatagg gatatgaata aaaaaaccac ggtattagta cgggtcttga tattatggga
      781 cgaaaatata tccttgggaa cgaagcgatt gcccacgcat gtcttgaatc gggcgttaat
      841 tttataagcg gataccccgg cacaccctca tctgaggtta tcgacacctt gcgtgctcaa
      901 ccgtatccat catactatct ggagtggtca gtgaatgaaa aggtggcatt ggaaaatgct
      961 cttgctgctg cctggtgtgg tatccgtgct ctgtgcacga tgaaacacgt tggtctcaac
     1021 gttgctgcag atcccctgat gacaagtgcc tataccgggg ttaccggagg tctcgtaata
     1081 ttgagtgcgg atgatccctt tgcccatagt tctcagaacg aacaggactc ccggtgctac
     1141 gcacattttg cccgtgtccc ctgccttgat cctgcaaccc tgcaggaagc tcatgatatg
     1201 atcccggaag cattcagaat ctccgaagaa ttcgggatac ccgtaatttt ccgtcctact
     1261 acaaggatct gtcattcaaa aggagatgtt gaactgaaag aagcagtagc agttcaccgg
     1321 gatggggaat ttcataaaaa tccccggaat tatgtcgtga tccccgcgca tacccgtgtc
     1381 ctccacaaaa tgctgaacga aaaacaatcc gcaatccgga aacggctggt tgaaaggggt
     1441 ttcaacaact accaaatacg tggtaagatt gcaatcattg cgagcgggat tggcgcgtct
     1501 tatgttcagg aaatattacc caatggtgtc tcttttctga agatcggagc gtatccgata
     1561 gacgaggaat ggctgggcga tttcgtccga aagcatgaaa gagtgctggt aattgaagaa
     1621 ctcgccccag aagttgaaga gattgtacgt caggtcgcgg gcaatatccc ggtatttggc
     1681 aaaaagaacg ggttcgcacc ctatgaagga gagttgtctc ccccactagt ggcagatatt
     1741 atggcgaaag ctggatttat aaaacaagat ccataccctc tgaatgatcc gatccaaaac
     1801 cttccccccc ggccgcctat cctttgtgcc ggttgtctgc accgcagtgc tttttatgcg
     1861 ataaaaaaag tttttaaaga tgctatatac cccagtgata tcgggtgcta tactctcggt
     1921 ctccagctcg gtgtggtgga cactacgatt tgtatgggtg catctatcac tgtcgcgagc
     1981 ggtatgtcgc atgcgggtga tccgcgtgat attgtctgta ccatcgggga ctcaacgttc
     2041 ctgcacacgg gtatccaggg actgataaac gcggtctaca atggagcaaa cataaccgtt
     2101 gtgattcttg ataaccggat taccgctatg acaggacacc agccgaatcc gaataccggc
     2161 ataaccgcgt gtggtgtgga aagtcccccg gtatcactcg atgcaatctg ccgtgcctgt
     2221 ggtgtggatt ttgtggagaa tgttgaccct tacgatatta ctggtatggt taatattctg
     2281 caggaagtta agaaaaggaa aggtgtgaag gttgtcattg ccaaacagat gtgcgtcata
     2341 tctgctcgtc gcgcaggtgt taaacgggga cgatatacgg tcaatgctga tttatgtaca
     2401 ggatgcggaa cctgcgtccg gttcggttgc cccgcaatcg aatttgccga tgaacatgcc
     2461 cggataaatg atctttgcag cggatgctcg gtatgtgtgc aactctgtcc gacaggggca
     2521 atagtaaggg aggccaagaa atgagcagaa gttttgatat cctgatagta ggaataggcg
     2581 gtcagggaac tatccttgcc tccaatattc ttggagaagc atgcctgctc gaagggaggc
     2641 atgtgaaagg ggctgaaacg catggaatgg ctcaacgagg tggatctgtt gagagccata
     2701 tccggataga tggattgttc ggcccgttga tcccaccggg ttacgctgat ctccttatat
     2761 cgtttgatct tctcgaagcg ctgcgctatt cccactacct gaaaaaagag ggtaaaatgg
     2821 tggtaaaccg gcatcttgtc ctgccgacat cagtctatac gcaaaagctt gtggtacctg
     2881 gagaagagga gatcattgcc gcattgaaaa agcatgtcct atgcctcatt gacgctgata
     2941 aaatcgccac tgaagcagga agcccgctct cgcagaacgt ggtaatgctg ggtgcagcgt
     3001 cgcaatcaat gccacttaaa cctgaatcgc ttttaatggc agtccggaat cttgttccca
     3061 aaaagacgat agatatcaat gtaaaatctt ttgagatggg aagggaattt ggcgggacat
     3121 gctgatgaaa cgtcagcttg gggcttttga tcctgatacg atttatcaag gtaatgccct
     3181 ccagctgatg cctctggttt ctgatgggat aattgacctt atcattaccg atccgccttt
     3241 tgctatcgat tttaaggctc gacgggaaaa ttacaaccga actggttcga atgttttaga
     3301 gggatataag gagatccctc cggcagagta ccgcgaattc acgcaaaaat ggatagcaga
     3361 agctgcacgc gtgcttgccc ctgacgggag tatgtatgtt ttttccggct ggaaccgcct
     3421 caaagatatt cttgaaggac tggatgcagc tggattcacc acaatcaacc atattatctg
     3481 gaagtatcaa ttcggcgtct ttacgaaaaa aaaatttgta accagtcatt atcacgttct
     3541 gtttgtggta aaagatccaa aacggtacac cttcaataag gtcgatcatt atgccgaaga
     3601 tgtatgggtg attaaccggg aatactggaa aggaaaaatc aagactccga ccaaactccc
     3661 ttcagaactt attaaaaaga tactcctgta ttcgagtaat cacggcgact tggtcttcga
     3721 tccattcctt ggttcgggta cggtagcaat cgtttcacag gcagaaggaa ggcattttct
     3781 cggttttgaa atcgtgcccg aatattttgc attcgcaaat gaatgtctgc gttcccaaag
     3841 aaaaaggatg taaaacaggt gattgaacgc tgaggggggg aattgaaccc ccgaggcact
     3901 ttcgcaccac aggctttcca ggcctgcgcc ctaccgctag actacctcag caaaaagtgt
     3961 atcctatctg ttagacgggt atttttttaa tggtatctct ttttttggaa ttttttttgg
     4021 tttttcatcc gggtccttcg cttttaccga ttttgtccag cctcgggtat aggtaccagg
     4081 ttgcataaaa actgtcgtag gtctgatgat aagccctgta tctcctggtt taaacgagct
     4141 ggaagtgaca attgccttgc cgagacagac aaattccttc ttctgtgata aaacagcaac
     4201 gacctgatcc tttttaaagt catcacactt tatcacacca attcctgcaa gtgtcgctcc
     4261 atggcagatg gcatcaatag ctgtatcccg gataattacg gtgggcagat cgggtactgc
     4321 tgcatcgact gacaatatca tcgacataag ttcagtgaga tttccctttt ttgccgaact
     4381 gcaggcatcc tgaacatcat ggagcgtatg catttcctgt tcactgaaag acccggaacg
     4441 tgtgcggcga agttccacca tgtgtgcacc aacacccagt gctagcccaa tatggtgaca
     4501 aagggaccgg atatacgtac ctgcatcgca ttgaactcgg aaaatgacga gacgtcctga
     4561 tttgccgagg atctcaagtt tctggatggt gcgtatccgg aggacgcgct tgacagcgct
     4621 ctttcgcggt ggacgctgat agagtcttcc ggtgaactcc gcaccgatct gttccatccg
     4681 gatttcgtca acatcgccat gcagccgcat aacgcagata tattccttat cgtgctggag
     4741 gagaagcggc gcgaggcgca ctgcattacc gagcatgatg agaagcactc cggaaacctg
     4801 cgggtcaagc gttcctgcat gccctacctg gcagccgagc atcttcccta cccacgcggc
     4861 tacctgatgg cttgaagggc ctcttggctt atcaaccacc ataatcccgg catgattcca
     4921 cagatggagg agttctcgcg tatcaggatc tccggtcatt ctatccactt ccctgtctgg
     4981 tgagatattc gttgagtgct gtcaacgatc cctcgagcga accatctccg attacaatcg
     5041 ggtccgttcc cgattcccgc attttcgctg cagtaatatc gccgattgcc atcacaataa
     5101 gatccgggtt cttcttccac actgcctttg tatatgacat tgtgcttgta aaaaggactg
     5161 catccgcctc gtcaaggttt agagactctc cggtcggctg aagtgaataa catttaaatt
     5221 cgtggggaat ccctcccgct gcagatattg catcgattag ggagtgattt ggaacatcag
     5281 cacgcgggat accaatatgt ttacccctga cccagtctcc aagatatggc acaaaatctc
     5341 ttgaatagaa acttgtcaga gtttcacaat cgatgttaaa gtgcaaaagt tcttttgcag
     5401 tctggggtcc gattgcgatt atgcggggat acctgtttag tagtggagca atgattttag
     5461 ccggaagcgc gctggtgaag aacaggcagt caaattcacc acgttcaacc gcttcgataa
     5521 acaattttat tgcgtgggac ctgatttccg aatgcaaagg gtgcacacta taacagatat
     5581 gaccgaattg tgcgcagcgg gcggcatcgt ttttttcctt gcctgtaagc cgggtaactg
     5641 caatcttcat tacggattca tcacctcatc atgatatgat ggtatgcatt tcggtgaatt
     5701 tatgtccact ctcctatatc cttagtgaat gattgagatc ctgctcggtg tttttattgg
     5761 cgttgtgctt ggtactataa gcgggatcat ccccggtgta catgcaaaca ccctggccgg
     5821 tgtgctctta agtctgcagg tagtgcttct ttcgtttttt ggacctctgg ttcttgcggg
     5881 tgcgatgttt gcagctctta taactcacac atttgtggac tccgttccaa gtacattcct
     5941 gggaattccg gatgctgaca catcagttgc cgtacttcct gcccatgctc tctgtcttga
     6001 aggtaagggt gaggaagcgg taaggattgc agcacttggc agtgccggtg caatgattat
     6061 tgcaataccg ctttccgtaa tatgtttttt tctcctccct gctttccagc cctatttcga
     6121 ttggtggatc ggtattctcc tggttgctac catgggttat atgatcgtta cctctgagtc
     6181 ccccggttgg gcacttacac tattttgtat cactggaatg ctgggttttt tcaccctcaa
     6241 ctataccttt ctcagctggc acacactcgc gggaaatagt gcaattctca tgccgctcct
     6301 caccgggctt tttgggattt ccgtcctcct gactgcatcg aagggtacta tgcctgaaca
     6361 acattaccag ggcatcagta tggaagacag gactattatg aaatattccg caatcggaac
     6421 aattgcgggt gttgctgtcg gatggcttcc cggactctct acagcctctg caaacagtgt
     6481 gctggcctcg cttataggat atgagaagaa ctcccggaca tacattcttg caacaagtgc
     6541 tgcaaacaca tcaaacgcgt ttataggact tgctgcgctc ttcgcactgt caagaatgcg
     6601 caatggtgtt atggtcgccc tttctgagct cccgttaccc actatgagtg aactgactgt
     6661 tgtcggtgtt cttgctgctt gtgcagccta tgtgattacc gtaatgctat ccggttctgc
     6721 gggcaggctt aacggaatcg acagtcgatc gatgaatcgg gcggttattg cctttatcgt
     6781 aattctatgc attctcctca ctggtccatt tggtgtaatt atactgattc ttgccactgc
     6841 gctcgggctt ataccgcaat tagtaaatgt gccaagagtt ttctgtatgg gtgcgattat
     6901 tgtcccggtc atattctatt cctttggttt tgcatggatt tgatttatta atcattcgga
     6961 tccgtaactc cctttgatct atggttttat agggacacga accctaaatt ttcctgaatg
     7021 cagtcttatt ggttcggtga tgtgaatgaa aggtgctgca cattgtttat gggagatttg
     7081 aatgccatca tcgatcgtgt gcaatcaatt tccacaagtc aggataatat aatacccctg
     7141 gattggcaac aggcaattct ttgtggtgcc tgtcatgatc gaacggatta tctcgctaaa
     7201 ttacaggaaa tttgcattac cgctgctgag cgttcaatac gagaacagta ctcaaggaaa
     7261 gatgcagaac tgcttcagat ggtaaggaca cttgatgaat ttgacaaagt catcaacctg
     7321 ctctctgaaa gggcagcaga atggtatacg gtaaggcacc cggctttttc tcgcaagtat
     7381 cgcaggacac cagcacattt gctggtaaaa accatgcgcg agaaaaatag aggcgcgctt
     7441 ggcagtgtag caggggagat tgaacgactc acagaaaccc gaactattct ggcaaaagag
     7501 gtttcggccc gggcgaataa tgtaatgccc aataccagcg ccctcattgg cgggcttgtt
     7561 gctgcccgcc tcctgtcgca tgccggaggg cttcttttac tctccaggct tccggcaagt
     7621 gcgatccagg tacttggtgc ccgtacggca ctgttttccc atctcaaaac acgatccccc
     7681 tgccctaagc atgggatcat ctttcaacac cgccgggtgc ataatgcacc ccggagtgtg
     7741 cggggaaaag tttccagggt tcttgccgga aaactagcga tcgcggcacg gctcgattat
     7801 ttcaggggag tgttagtacc cgattttatt gatgccgcac aggagcgcat tgatagtaca
     7861 ggcaaacgag gtgaatcttg atctggattg gtgatgtttt agtatctcat ggcgcgggcg
     7921 gagtatacaa cgaacgatta ctgaacggcg cccgggtatg ggaccccaac cggagcaaac
     7981 tgtctgcact ctatcatata gggaccggaa ttgagctgac atccgggatg cgcgtgttgt
     8041 atctgggagc ggcgaacggg acaacagtat cccatgtggc ggattatgtt gaggtggtat
     8101 atgctgtaga gttcgccccg cgccccatgc aggatctcct tgaagtatgt cggcggagaa
     8161 ggaacatcat ccctattatg gcagatgcat cccgccccga gtgttatgtt ccattggtcg
     8221 aatccgttga cctgatctat caggatgtcg cccagccgga tcaggcttct atcgcactgc
     8281 ggaattgttt gttcttaaaa gaaggcggat ttttaattct catgctgaag acacggagtg
     8341 ttaatgtaag aaaggaacct gctgaagtgt ttcaagatac cctcacttcc ctgacttcag
     8401 gagggcttat tattcaggaa agtgtatggc tgaccccgta tcatcacgat catgcagcaa
     8461 ttatctgtaa gaaacccggt gtatgaggaa tcgtcatgag cgataaggtc cgtttctttt
     8521 ctgccggtcc gatttctata ctcacgattg tcctcctcgt tgggcttgca atcatcctca
     8581 tccccttact gattcttggc atcataggag cagctttcac gaagctgggg ttttcttgga
     8641 tttcagccct cgctgtcgtc ttttgtgtgg tgttcggaag ttttgtaaat atccctttgt
     8701 atcatataca gcgcgatgtg gtccaggcct accgaaatga gacttcagac tttgatgtct
     8761 gtgtcccctg cgcctccact cccgtctggg atacattagt atccataaac cttgggggcg
     8821 ctgtgattcc aacatgtgtg tcgctataca tgttgtacgg gacgatcagg ataatgggag
     8881 cccccctgat ttttaccgtc ttcgtggcaa tagctctcgt ggggataatt gcgtttattt
     8941 caacccgcct cgtaacgtat tccgggatac aagtacctct gctgattccg ggccttacag
     9001 cacttcttgc cggtttggtt cttacaggtg ggaccggccc tacagcggca gtaacagcat
     9061 ttgtgagtgg gacgaccggg gttttaatag gtggaaacat tgccaatttt taccgtatta
     9121 aaaatctcga agtaaaagcg gtcagttttg gcggtgccgg tacgttcgga tctattttca
     9181 tctgctgcat gctcccggcc ttaattgcat aggcgaaaac agcagccaga aaagaacaca
     9241 tctttctgga tataagaaaa accactatga taagaagtag cccggagcag attcgaactg
     9301 ctgtcggggg atccagagtc ccccatgatt gaccgctaca ctaccgggct ttgtgcttct
     9361 tattttttac cgctgtttct taattaaggt gacggactgg cccttgtcat aattcttttg
     9421 cccgttcaca cttccggcag gcatgacata cttccctggc aggctgaata atctcaccct
     9481 ttttgcagaa gggttcgata tcattgatat cccgaatata gtaaatgtgg gggataaggg
     9541 aaatgtgagt atatgaaccg caggaggcgc caagagaatc tgcaatagat ttctggagtt
     9601 gtacaagggc atacatattt gcacctgccg cggtgagcat atcatttgaa cggaatatta
     9661 cccgcatgtt aagtttacgg tcccgcagga cgcactgtac gagctggaga cagggacaat
     9721 tatcaagcat ctgatcgatc acagggttcc aggttatagc tatcgcacgc cgggtagaag
     9781 gagatgattt gattttttcg acgatatagg agatctggtc gatatgcaca ggctttccgt
     9841 cagcaacaag gcgctcaccc cagtcaaaga gccggccgtg atagtcatac tcgaatgaag
     9901 catgtgaacc gtgcaataaa tctctggcat attgctccac aaatcgcttc tggaatcggg
     9961 agtcaggact caccgctggt tctgcgagag gagtatctac ctgcaatgta acttcttcga
    10021 attcgatagt tgcttcatca tcttcagttt gaagaatcca gcccttctcc aggatcattt
    10081 tgatgaccag ttcatgtgcc cgcccgatgc ttggcgcccg gataattctc atggaaaatc
    10141 gtgtgtcatt atacattgta tatgtgttga aatgttgttt aatcgaaatt gaattggatg
    10201 aacctcaaag atgaaattag atatattgaa agctgaaaaa ggaattaaca tttgaactga
    10261 cgatattatt aggttaaatg aacactatat ctgtcatcat aaaaattatt gtgaattcag
    10321 taataagaat taacgaaaac actataaata aatctaataa attattataa tatagtatat
    10381 ttatatttgt agcattttgg gtagtcgggg atgttaaata atgagccaaa atccatttta
    10441 atcgctcaaa actataaaca gagtccaaat attgcccaga tttacctaaa aaac
//
LOCUS       DASRLV010000073         6924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_187339,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000073 DASRLV010000000
VERSION     DASRLV010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 6924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6924
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187339"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VFC43_03010"
     CDS             <1..450
                     /locus_tag="VFC43_03010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HZK30188.1"
                     /translation="YKGGSAKIICAVKGDKSLHLFDTFEGLPKVDDIDMVWPFYEGKF
                     AASYDSVKDYLKNDLNVFFYKGIFPGTSGPVQEKTFSMVNLDVDTYESTKQCLEFFYN
                     RMNQGGILISHDYMTAPGVKKAFDDFFDGKAEPVLETAGSQCIVVKV"
     gene            complement(721..900)
                     /locus_tag="VFC43_03015"
     CDS             complement(721..900)
                     /locus_tag="VFC43_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30189.1"
                     /translation="MDKMGLVALIVGLVLCIAGGYAIWAFLPDVITAVKGLIGIVVLL
                     AGLMLAVFGLLIVND"
     gene            complement(977..2080)
                     /locus_tag="VFC43_03020"
     CDS             complement(977..2080)
                     /locus_tag="VFC43_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK30190.1"
                     /translation="MPVLPAILPLLIATNGYSYLAAGLLVTAYNVTSSCTQPLVGWLS
                     DTKGFSVSISISLLVSAVFIALMGVAQSYYLIMFFAIIAALGHACFHPTALSLVNRLC
                     IQENRGRITSFFVVGGNFGYAIGPVLAGALVFWLGLPGLLFLIFPALFMVVALKYILP
                     GGIAGACVSHALPVTRTVERTSKMPFIILMTASVLRAWAVFAAITFLPMYLVSQGYDL
                     VTASMIMTLMLLSGVAGQMAGGYISDMIGRKEFMIFGLAGAVPFFYLFFSSSGTIAII
                     TLLIFGFFLWSTFAVAVAMSHELLPRNIGLASGMMLGLAIGVGGLGVAVNGIIADRYS
                     LAVALGTIPVPILAALLFMVILPYPWKSFKGIP"
     gene            complement(2149..2763)
                     /locus_tag="VFC43_03025"
     CDS             complement(2149..2763)
                     /locus_tag="VFC43_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="METTL5 family protein"
                     /protein_id="HZK30191.1"
                     /translation="MKLKRLEMILQRLSGYTHPRAGLEQYQTPAPLAARLLYHALMKG
                     DIKGKTVCDLGCGTGVLAIGAALLGADSVKGVDSDPGAVRVAMENAELLDTQVGFIVA
                     DVKDEAIRGLLGSCDTVIMNPPFGAQKAHADRPFIDLALRIAPVTYSIFNAGSAQFIE
                     TYTAQRAKIDEKVGGVFPLRRTFSFHTQDVKEIEVEILRLIRKQ"
     gene            complement(2760..3707)
                     /gene="dph2"
                     /locus_tag="VFC43_03030"
     CDS             complement(2760..3707)
                     /gene="dph2"
                     /locus_tag="VFC43_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043364 - glycyl-radical enzyme activating
                     activity [Evidence IEA];
                     GO_process: GO:0017183 - protein histidyl modification to
                     diphthamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthamide biosynthesis enzyme Dph2"
                     /protein_id="HZK30192.1"
                     /translation="MSLNGTSDLIQRLKKKGAKRVALQFPEGLKRRSVQVAADLKGAG
                     FDVILSGDPCYGACDLALDSLAMADVLVHFGHFPVDDQPEVIFEPYTIDFDITVLKNA
                     IPFLKGNTVVLVTTAQHVHLITEMETFLQAEGIACIVTKGSGRTPNRGQVLGCSFNAA
                     KACGTDEVLFVGTGLFHPIGIALATRLRVTALDPVTGLAQEVNGDVLLRRRFAVMEKA
                     RDARNAGIIVSTKSGQQRMDLARRLESLSPTAVIVTMQDVSPDELLNLGFDCYVNTAC
                     PRLAYDDQVRFPVPVLSPQEFEILHGVRTWDEYTIDEIV"
     gene            complement(3685..4233)
                     /gene="hpt"
                     /locus_tag="VFC43_03035"
     CDS             complement(3685..4233)
                     /gene="hpt"
                     /locus_tag="VFC43_03035"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine/guanine phosphoribosyltransferase"
                     /protein_id="HZK30193.1"
                     /translation="MLDRLVTSLETCPMIKRGEYNYFIHPITDGVPIIEPALLREVCT
                     AMVKMLDLTGVDTIVVVEAMGIHIGSILSTMTDIPMTIMRKRAYRLPGEIAVHQTTGY
                     SKGELYLNGIKKGDRVVIIDDVVSTGGTMKALLQALAIAGADVVDVCIAVQRGDPDIG
                     RPYKSLVKIEVTDRVYVVERHI"
     gene            complement(4226..5326)
                     /gene="mfnA"
                     /locus_tag="VFC43_03040"
     CDS             complement(4226..5326)
                     /gene="mfnA"
                     /locus_tag="VFC43_03040"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase MfnA"
                     /protein_id="HZK30194.1"
                     /translation="MLNKGRSEEELFSFFAQKKSKDMDHNYILSSMCTHPHPVAVRAH
                     CMFMETNLGDPGLFLGTAALEKLLIERLGTLFHCTEAGGYATSGGTESNIQALRLAKA
                     IKKEVVYPNIVVPESAHFSFKKACDLLCLEMRTVPLGEGFRMDREKAAEQIDKNTVCL
                     VGIAGTTEYGMIDPIADLARIAVAHDIFFHVDAAFGGMVIPFLKNPVPFDFSLPGVAS
                     IAVDPHKMGLSTIPAGCLLTREPDILKNLNIDTPYLSVKQEYTLAGTRPGAPVVGALA
                     VLDYLGVEGMQAVVSGCMKNTGRLIAGMETFGIPRVITPDVNVATFTCRNVPKPWKVS
                     WTRQGHLRIVCMPHVHGDRIEAFLKDVGELYA"
     gene            complement(5316..>6924)
                     /gene="ppsA"
                     /locus_tag="VFC43_03045"
     CDS             complement(5316..>6924)
                     /gene="ppsA"
                     /locus_tag="VFC43_03045"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HZK30195.1"
                     /translation="PDKYIFDQRSEKVEDILISNKKVEIIADGDHGTKLVEVTGARQD
                     TQVLSSAEITKLAMYGKIAENHYGIPQDVEWGIVSGTIYILQSRPITTIGNKKEAKSR
                     PGQKQNAKIILQGQGAAPGIASGRVAIIRDVKDTGRVKEGDILVTKMTSPDMVPAMRK
                     VAAIITDEGGLTCHAAIVSRELGTPAVVGTKTATHVLTNGQLITVDGEKGLIYEGEIT
                     APAKPAQVVQMAAAAPHIVTATSVKVNVSIPEAAARAAATGADGVGLLRIEHLILGLN
                     KTPGWFIANNKEEDFVRELHDGIKIVLDAFPGKPVWVRTLDAPTDEFRNMMGGENEPH
                     EHNPMLGWRGIRRDIQSPDQFRLQVESFKRLWKEGYENLGIMFPMVSHPDEFIAAKAM
                     MKACGVDVENVTLGIMIEIPASAIMIEEFLKCGIKFASFGTNDLVQYTLAIDRNNENV
                     ADMYQPQHPAVLHLIHTAIQMCRAYNVECSICGQAGSDPKMATWLVEHGISSISANID
                     AIAKIREAVARTEKRIILEAARTRDAE"
BASE COUNT         1682 a         1824 c         1746 g         1672 t
ORIGIN      
        1 tataaaggtg ggtcggcaaa gattatctgc gcggtgaaag gggacaaatc cctccacctc
       61 tttgacacgt ttgaaggctt gccaaaagtg gatgatattg atatggtctg gcctttctac
      121 gagggaaagt ttgcagcatc gtatgatagt gtcaaagatt acctcaaaaa cgacctcaat
      181 gtcttttttt ataagggtat cttccctggc acatcgggac ccgttcagga aaaaacattc
      241 tcgatggtaa atcttgatgt tgatacgtac gagagcacga agcagtgcct cgagttcttt
      301 tataatcgta tgaaccaggg agggatcctc atctcccatg attacatgac cgctcccggt
      361 gtgaagaaag cgtttgatga cttttttgat ggcaaagccg agccggtact cgaaactgcc
      421 ggatcgcagt gcatcgtcgt caaagtgtga caagctcact cttcatcgca gtgttttttc
      481 ttaagttttt ctcctgacca gatattcaga tttgagatgg cctgacaggt atttcaaaga
      541 cacgggcaaa gattttaccg ctaatcctgt ttctggaccc gaaaaatggt gataataacc
      601 atccggtatg attaccctga tgagaagcac ggaagagtga ggggaatgaa atcccggcag
      661 gagaccagaa caggattgtg tttctaacgg gccggtattc ccttttttta gtcccggctt
      721 ttaatcgttg acgattaaca ggccaaatac cgcaagcata agaccggcga gtaatacaac
      781 gatacctatc agtcctttta ctgcggttat tacgtcgggg aggaaagccc agattgcata
      841 gcctccggcg atacataaaa ccaaaccgac aattaatgca acaagtccca ttttatccat
      901 aataatccct caatctctct tgatttgtga atcagtccat ataagtgtat tgaatcctcc
      961 cttatggaca atcccctcac gggattcctt tgaatgattt ccagggatat ggaagtataa
     1021 ccataaacag cagcgctgca agaatcggga caggaatagt tccgagtgcc acagcaaggg
     1081 agtagcggtc agcgatgatc ccgtttaccg ctactcccag ccccccgacg ccaattgcaa
     1141 gaccgagcat catgccggaa gcaagcccga tgttccgggg aaggagctcg tgtgacatag
     1201 ccactgcaac agcaaatgtc gaccagagaa agaacccgaa gatgaggaga gttatgatcg
     1261 caatggttcc cgagcttgaa aagaacaaat aaaagaaagg aaccgcaccg gcaagcccga
     1321 agatcatgaa ctcttttctg cctatcatgt cagagatata cccgcctgcc atctggccgg
     1381 caacacctga gaggagcatg agagtcatga tcatgcttgc tgtcacgaga tcatatccct
     1441 gtgaaaccag atacatcggc aggaaggtaa ttgcggcaaa aaccgcccag gccctgagaa
     1501 cggatgcggt catgaggata ataaaaggca tttttgacgt tctctccacg gtccgggtta
     1561 ctggaagtgc atgagataca catgctccgg cgattccccc ggggaggata tacttgagcg
     1621 caactaccat gaaaagcgcc ggaaagatca ggaacaggag cccggggagc cccagccaga
     1681 ataccagggc accggcaaga accggtccga ttgcatagcc aaaattcccc ccgaccacga
     1741 aaaacgaggt gatacgcccc cggttttcct ggatgcagag acggttcacc aggctcaggg
     1801 ccgtggggtg aaaacatgca tggcccagtg ctgcgatgat ggcaaagaac attatcaggt
     1861 aataactctg tgcaactccc atgagggcga tgaatactgc actgactaac aggctgatac
     1921 tgatgctcac ggagaaaccc ttcgtgtccg agagccagcc aacaagtggc tgggtgcacg
     1981 atgaggtcac attgtatgcc gtgacaagca gcccggccgc aaggtaggaa tacccgtttg
     2041 ttgctatcag aaggggaaga attgcgggaa gcacgggcat gtaaatatcc gtgacaagat
     2101 gtgctgccgc aagtcctgat atggtatttt tgacactgtt ttttttgtct attgtttccg
     2161 tatcagccgt aggatctcaa cctcgatctc cttcacatcc tgcgtatgga aggaaaaagt
     2221 gcgccggagc ggaaatacac ccccgacttt ttcatcgatc ttcgcacgct gtgctgtgta
     2281 ggtttctata aattgtgcag atcctgcatt aaagatactg taggttaccg gtgcaatacg
     2341 cagggcgagg tcaataaacg ggcgatccgc atgagctttc tgtgccccga atggcgggtt
     2401 catgatgacg gtatcacagg aacctaaaag cccccgaatt gcttcatcct ttacatcggc
     2461 gacaataaac ccgacttggg tatcgagcag ttcagcattc tccattgcaa ccctgacagc
     2521 accaggatca ctatcaacgc ctttcacact atcggcacca aggagagcag cgcctattgc
     2581 gagaacgccg gtaccacatc ccaggtcgca gactgttttt cctttgatat cccctttcat
     2641 gagcgcatgg tacaacaacc gggctgcaag cggggcaggc gtctggtact gctcgagtcc
     2701 agcccggggg tgggtatacc cactcaaccg ctggagtatc atctcaaggc gtttgagttt
     2761 catacgatct cgtcaatcgt atattcgtcc cacgtacgga ctccgtgcag aatctcaaat
     2821 tcctgtggtg aaagaaccgg taccgggaac cgcacctgat catcatacgc gagccgcggg
     2881 cacgcagtat tcacataaca gtcgaatccc aggttcagca actcgtcggg ggatacatcc
     2941 tgcatggtta cgatcacggc cgtgggggac agggattcca gtcgccgggc aaggtccatt
     3001 ctctgctggc cgctcttggt gctgacaatg attccagcgt ttctggcatc tcttgctttc
     3061 tccataaccg cgaatctgcg acgcaggagc acatccccgt tgacttcctg ggccagtccg
     3121 gtgacaggat caagggctgt tacgcgcaaa cgggtagcaa gcgcaatgcc aataggatga
     3181 aagagaccag ttcccacaaa aagaacctca tctgtaccgc atgcttttgc cgcgttaaaa
     3241 ctacacccga gcacctgccc ccggtttggg gttcggccgc tcccttttgt tacgatacag
     3301 gcaattcctt ctgcctgcag gaacgtttcc atctctgtga tcaggtggac atgctgggca
     3361 gtggtgacaa ggaccacggt gtttcctttc aggaacggga tagcattctt aagaaccgtg
     3421 atatcaaaat caatagtata aggttcgaag atcacctctg gctggtcgtc gacaggaaaa
     3481 tgcccgaaat ggactaaaac gtctgccatt gcaagactgt ccaatgcaag atcgcaggcc
     3541 ccataacagg gatcaccact gaggatcacg tcaaaacccg cacctttcag atcggcggct
     3601 acctgtaccg aacgcctttt caacccttcg gggaactgga gtgcaacacg ttttgcccct
     3661 ttcttcttta atcgttgaat caggtcagat gtgccgttca acgacataca ccctgtcggt
     3721 cacttctatc ttgaccagtg atttatacgg gcgtccgata tcaggatcgc cacgctgcac
     3781 cgcgatacat acgtccacca catcggctcc ggcaatggca agtgcctgca ggagagcttt
     3841 catcgtgcca cctgtgctca ccacatcgtc gatgatcacg acccggtctc ctttcttaat
     3901 tccgttgagg tagagttccc cttttgaata accggtagtc tggtgaaccg ctatctcacc
     3961 aggcaacctg tacgcacgct ttcgcattat ggtcataggg atatcggtca tcgtagacag
     4021 gatcgatccg atatgaatgc ccatcgcttc aaccacgaca atggtgtcaa caccggtcaa
     4081 atcgagcatt tttaccatcg cggtgcatac ttcacggagc agggccggct caataatagg
     4141 tacaccatcg gtgatggggt gaatgaaata attatactcg ccccttttta tcatagggca
     4201 ggtctcaagg gaggtcacaa gtctgtcaag catacaattc acctacgtct tttaagaatg
     4261 cctcaatgcg gtcgccatga acatggggca tgcagactat ccgcaggtgt ccctgtcgtg
     4321 tccacgagac tttccagggt tttggaacat tccggcaggt aaatgttgcc acgttaacgt
     4381 caggagtaat gacccggggg atgccgaatg tttccatacc ggcaatcagg cgcccggtat
     4441 ttttcatgca tcctgaaaca actgcctgca tcccttcaac tccaagataa tcgaggacgg
     4501 caagcgctcc cacaacagga gctcccgggc gggtgcccgc cagcgtgtac tcctgtttta
     4561 ccgagagata cggggtatcg atattgaggt ttttgagaat atcaggttcc ctcgtgagca
     4621 ggcaccccgc tgggatggtg ctcaggccca tcttgtgggg gtcgactgca atggatgcaa
     4681 cgccgggaag ggaaaagtca aaaggaacag gatttttaag gaatggtata accatgccgc
     4741 caaaagctgc gtccacgtgg aaaaaaatgt catgggcaac tgcgatccgg gcaaggtcag
     4801 ctatggggtc aatcatgcca tactcggtgg ttccggcaat gcccacgagg cagaccgtgt
     4861 ttttgtctat ctgctctgct gctttttccc tgtccatgcg gaacccctca ccaagcggga
     4921 ccgtccgcat ctcaagacag agaaggtcac aggctttctt gaacgagaag tgcgctgatt
     4981 ccggtacaac gatgttggga tacacaacct ctttttttat ggcttttgcg agacggagag
     5041 cctggatatt tgactcggtt ccacccgatg ttgcatagcc tccggcctct gtacaatgga
     5101 aaagtgttcc cagccgttca atgagaagtt tttcgagcgc tgcggtaccc aaaaacagcc
     5161 cggggtcacc aagatttgtt tccatgaaca tgcaatgtgc cctgacagcg accgggtggg
     5221 ggtgggtgca catcgagctt aaaatgtagt tgtggtccat gtccttggac tttttctggg
     5281 cgaaaaaaga gaatagttct tcttcggaac gacccttatt cagcatcgcg ggttctcgca
     5341 gcctcaagga tgatacgctt ttctgtacgg gcaaccgctt cgcggatctt cgcgattgca
     5401 tcgatatttg ccgatatact ggatatgcca tgctcaacaa gccaggtggc catcttcgga
     5461 tcagaaccag cctgaccgca gatggagcac tcgacattat atgcccggca catctggatt
     5521 gccgtatgga tgaggtgaag aactgcgggg tgctggggct ggtacatatc cgcaacattt
     5581 tcattgttac ggtcaattgc gagggtgtac tggacgagat cattggtccc gaatgatgcg
     5641 aacttaatac cacacttgag gaattcctcg atcatgatcg cgcttgccgg tatttcgatc
     5701 attataccaa gggtgacgtt ttcaacatca accccgcacg ccttcatcat ggctttcgcc
     5761 gcgataaact cgtccgggtg ggagaccatc gggaacatga tgccgagatt ctcatacccc
     5821 tccttccaga gacgcttgaa cgactcaacc tgaagacgga actgatccgg gctctggatg
     5881 tcgcgccgga ttccccgcca gccgagcatg gggttatgct catggggctc gttctctccc
     5941 cccatcatat tccggaattc atcggtcggg gcatccagtg tccgcaccca caccggttta
     6001 ccggggaacg catcgagtac tatcttgatg ccatcgtgga gttccctgac aaaatcctcc
     6061 tccttgttgt ttgcgatgaa ccagcccggt gtcttgttga gcccgaggat caggtgctct
     6121 atccgcagta atccgactcc atctgctccc gttgctgcag cccgtgctgc tgcttcggga
     6181 atggagacat tcacctttac acttgtcgcg gtgacaatat gaggggcagc agctgccatc
     6241 tgcactacct gagccggctt tgccggagcg gttatctcac cttcgtaaat gagacctttc
     6301 tcgccatcaa cggtaataag ctgcccgttc gtcagaacat gggttgcagt cttcgtgcct
     6361 acgactgccg gtgtccccag ttcacggctg acgattgcag catggcaggt cagacccccc
     6421 tcatcggtga taattgcggc gaccttgcgc atcgccggta ccatgtcagg gcttgtcatc
     6481 ttcgttacga gaatgtcgcc ctcttttacc ctaccggtat cttttacgtc ccgaatgatg
     6541 gcgaccctgc ccgaagcaat cccgggagct gctccctgtc cctgcagaat tattttggcg
     6601 ttctgttttt gtcctggcct gctctttgcc tctttcttgt tcccgattgt cgttatgggg
     6661 cgggactgga gaatatagat cgtcccggat acaatgcccc attcgacatc ctggggaata
     6721 ccataatggt tttcagcaat cttgccgtac atggcgagtt tggtaatttc tgcagaagag
     6781 aggacctggg tgtcctgccg ggcacccgtt acctccacaa gtttggtacc atggtccccg
     6841 tcagcaataa tctccacttt cttatttgag atgagaatgt cttccacttt ttccgaccgc
     6901 tggtcgaaga tgtatttatc cggt
//
LOCUS       DASRLV010000074         9058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_21951,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000074 DASRLV010000000
VERSION     DASRLV010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 9058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9058
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21951"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VFC43_03050"
     CDS             <1..565
                     /locus_tag="VFC43_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division control protein Cdc6"
                     /protein_id="HZK30196.1"
                     /translation="MEGAIAEGVIPLCSALAAQEHGDARRALDLLRISGELADRDESN
                     QVKEEHVKQAQAKIETDSMIECIATIPTQSKLILFSMLILEQLGQNIFTSGEVSRIYQ
                     DIAPRIELDVLTHRRITDLISELNMLGVINTRVVSRGRYGRTKEMWFDSNTGKIREVV
                     LKDSRLNGLKDLDVKQMETKWLKTWFR"
     gene            572..1063
                     /locus_tag="VFC43_03055"
     CDS             572..1063
                     /locus_tag="VFC43_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HZK30197.1"
                     /translation="MQEKDLELLQILENNSRLSATEIATMTNLTPADVESRIHALETA
                     GVICKYTTVINWEKAGNGEVSSIIELKVSPERDFGYDRIAERLSRFRQVKTLRLMTGT
                     YDLQLLVTGKNMQEVSRFVSEHVAPMDRIRETATHIIMKSYKENGNVLFEQQEAERLP
                     YSF"
     gene            1073..2236
                     /locus_tag="VFC43_03060"
     CDS             1073..2236
                     /locus_tag="VFC43_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZK30198.1"
                     /translation="MRNFISERAHNIPPSGIRKFFDLALTIEDVISLGVGEPDFRTPW
                     NICESSIYSIEQGVTSYTPNKGLPVLRESLSNYLQQHYHLPYNSDNEIIITSGVSEGL
                     DIAIRAIVDPGDEVLIAQPSYVSYAPCVTLAGGIPVPVQCTEPDRFKLNADALQDKIT
                     GKTKALMINFPNNPTGAVMNREDLRAISDIIIDKDLLLITDEVYAELTYEGSHVAAAT
                     VSDLWQRTITLNGFSKAYAMTGWRVGYLCAPKELCEAVLKIHQYVMLCAPVMGQVGAL
                     EAIRSAEEDKNNMVQEYRLRRNMFIAGLNRIGLPAHVPDGAFYAFPSIKETGLSDTEF
                     AERLLKEQRVAVVPGSVFGAGGEDHVRCAYAVSRKQLTEALGRIETFLTNLRN"
     gene            2338..3351
                     /locus_tag="VFC43_03065"
     CDS             2338..3351
                     /locus_tag="VFC43_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HZK30199.1"
                     /translation="MSCTIIVGGFFGDEGKGKIVAHIAYKDKPVIISRGGVGPNAGHT
                     VQVGEREYGVRMVPSGFVYKEAKLCIGSGVLVDPRILKQEVEMLDVKGRVFVDKRCGI
                     ITEDHIVRDKGSAHLSKKIGSTGSGCGPANSDRVMRLSPQAKDVPELAEYLLDVPKAI
                     DDELKQGRTVLLEGTQGFGISLYYGTYPFVTSKDTSASQIAADNGVGPTKIDDVIVVF
                     KAYPTRVGEGPFSTEMTFEKSDSMGIQEFGTVTHRKRRIGGWDGNMARYSAMINGCTQ
                     AAITGIDRVDKSCFGVTEYGKLTKKAKDFLKSAEDDIGCSITLISTGPEMTQIIDLRE
                     KQE"
     gene            3363..3533
                     /locus_tag="VFC43_03070"
     CDS             3363..3533
                     /locus_tag="VFC43_03070"
                     /inference="COORDINATES: protein motif:HMM:NF038101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methytransferase partner Trm112"
                     /protein_id="HZK30200.1"
                     /translation="MDILCCPVCKGDLSLNVEKEDEKEIIEGELNCAVCKVNYPIHEG
                     IPNLLPQTSQKK"
     gene            3549..4121
                     /locus_tag="VFC43_03075"
     CDS             3549..4121
                     /locus_tag="VFC43_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30201.1"
                     /translation="MNFASAFNILTVQDPGLHETSFEFYLNRLGINLIEIPRENVWAE
                     IGGTLRLIFKNRGAPTHLTISSPNAEMFTDFFHENMYIVDETVLTIPIRKDCQEGFFI
                     LEIITGYGGIKTSLRVDVIPGVVIQKSAVKQEPPLQPVAHGRPHLLMVMMGIALILYS
                     AFLYTKNEFLNTAAFIILIVGALYTWYRQQ"
     gene            4103..5044
                     /gene="cbiB"
                     /locus_tag="VFC43_03080"
     CDS             4103..5044
                     /gene="cbiB"
                     /locus_tag="VFC43_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HZK30202.1"
                     /translation="MVPSAVVAGVVVLFAALIVDRLIGDPQSPFHPVALLGWFIGKWG
                     QPRKYSPQMQRFAGVLLWMVTAALFALPFYYFAAVAPWFLYLVGAPILLKCCFAWRSL
                     EEHTLAVVHALHAGVEEGRDQVKMLVSRDTTHLNRAHILSAGYESMTENLTDSIISPL
                     FYFSLFGLVGAAVYRAANTMDAMLGYKDERKRLGWFSARMDDILNFVPARLTVMVLLV
                     YFAFTGRFSHAVRVMRRDGCKRPGFNGGIVMAAMAGGTGIQFVKPGVYEIGDNERTFD
                     DAGAEIIRAGRFATIAFTVLASGTLFLLGSLINSTGI"
     gene            5041..5937
                     /gene="pdxS"
                     /locus_tag="VFC43_03085"
     CDS             5041..5937
                     /gene="pdxS"
                     /locus_tag="VFC43_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HZK30203.1"
                     /translation="MKLEELRFGTELLKRGFASMQKGGVIMDVVNADQAKIAEEAGAV
                     AVMSLERVPSDIRKMGGVARMADPEKVIEIIDAVSIPVMGKVRIGHFVEAQVLETLGV
                     DMIDESEVLTPADEQYHIDKKLFKIPFVCGARDLGEALRRIDEGAAMIRTKGEAGTGN
                     VVEAVRHMRNIMGEIRLLKGMDRQEMIDYAREIEAPAELVIECAGRGRLPVVNFSAGG
                     IATPSDAAMMMQLGADGVFVGSGIFKSSDPERMAKAIVEAVNHFSDATVIAKVSKGLG
                     DAMPGLDVHTLREDEKLQTRGR"
     gene            5927..6517
                     /gene="pdxT"
                     /locus_tag="VFC43_03090"
     CDS             5927..6517
                     /gene="pdxT"
                     /locus_tag="VFC43_03090"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HZK30204.1"
                     /translation="MDVKIGVLALQGNVSEHIDAFRLALIRMGHTTSSEVFEVRNPED
                     LAGCDALAIPGGESTTISRLIDKNQMYEPIRSFSGGIFATCAGMVLVATHVDDERIHS
                     LGLIEITVDRNAFGRQRESFEADLSIECLEGGPFHAIFIRAPVATSAGPGVTVLSCMD
                     QGIVAVEYGKHIALSFHPELSCDTRLHERFLRELGV"
     gene            complement(6659..7372)
                     /locus_tag="VFC43_03095"
     CDS             complement(6659..7372)
                     /locus_tag="VFC43_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HZK30205.1"
                     /translation="MDPVITQIRQELKDHQDMEIRKTSQRFFKEEIACYGIKTATVLA
                     IAKKYWKVVRCRPKPEIFFLCEDLYKSGYLEESFIVSNWTSSLIGHYEPDDLSVFRNW
                     IDSYITNWASCDGFCNHTMGNFIEQYPEYIKELKCWTKSPNRWMRRASAVSLIIPAKR
                     GKFLYESMEIADLLLTDSDDMVQKGYGWLLKEASRKHGTEVFDFVLRNRKSMPRTALR
                     YAIELLPKDQKTEAMKKDW"
     gene            complement(7451..7954)
                     /locus_tag="VFC43_03100"
     CDS             complement(7451..7954)
                     /locus_tag="VFC43_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HZK30206.1"
                     /translation="MSARILVAYASRKGSTAGIAHAIGKELISKGYAVDISEMKSVTS
                     LVGFSAVVIGAPFYTGKVKGDVVAFAAAHKDAISRLPVAGFVTGIAPVYPKTGEVKEF
                     TDQLIVALAPSSPVSVTVFAGTLDAGKMNFIERCLTSLMKVPTGDFRDWIAIAAWARN
                     LPQKMGL"
     gene            7971..8417
                     /locus_tag="VFC43_03105"
     CDS             7971..8417
                     /locus_tag="VFC43_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30207.1"
                     /translation="MVGCITYKNRAKCPVQQPSVHCLAYRCFNSYLEPTEVFPLPLIM
                     QGVETNFAMVTLNPNSCSFCIFYGYVPHFMNLHIVLPGEDKSKGNNHDSCRGIRVHIM
                     ATDPVCFAVVDGARFTRIYKNQKCYFRTNYGKNKYDEVLRIFRDGL"
     gene            8534..8716
                     /locus_tag="VFC43_03110"
     CDS             8534..8716
                     /locus_tag="VFC43_03110"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HZK30208.1"
                     /translation="MPTDPVCFMIVDEDDAKFTSKYKDHEYYFCCNWCKKKFDENPKR
                     YWRSSSDISVNLSDVT"
BASE COUNT         2445 a         1982 c         2227 g         2404 t
ORIGIN      
        1 catggaaggt gcgattgccg aaggggtaat accgctctgt tccgcgcttg ccgctcagga
       61 acacggagat gcgaggcggg cccttgacct gttaagaatt tccggcgagc ttgctgatcg
      121 cgatgaatca aatcaggtca aagaagaaca tgtaaaacag gctcaggcaa agattgagac
      181 ggatagtatg attgaatgca tcgcgactat tcccacacag agtaaactta tacttttctc
      241 aatgctcata ctcgagcagc tgggacaaaa catcttcacg agtggggaag tttcacgtat
      301 ctatcaggat atagctccga ggatcgagct tgatgtactt actcaccgcc gcattaccga
      361 tctaatatct gaattgaaca tgctgggtgt aataaataca cgagttgtga gccgtggccg
      421 gtatgggaga acaaaagaga tgtggtttga ctcaaatacc ggcaagatcc gcgaggtcgt
      481 attaaaggat tcgcggctga atgggttaaa agatcttgat gtgaagcaga tggaaacaaa
      541 atggttgaaa acatggttca ggtgatgaag aatgcaggaa aaagatcttg aactactcca
      601 gattcttgag aacaacagtc gtctctctgc aacggaaatc gcaacaatga ccaatctcac
      661 cccagccgac gtagagtcac ggatccacgc acttgagacc gcaggagtaa tctgtaaata
      721 tactaccgtc atcaactggg aaaaagcagg caacggagaa gtctcttcta tcattgaact
      781 gaaagtgagt ccagaacgcg actttgggta tgaccggatt gcagagcgtc tgtcacggtt
      841 ccggcaggta aaaacgctgc gccttatgac cggtacttac gatcttcaac tgctcgtaac
      901 cggaaagaat atgcaggaag tttcccgctt tgtttctgaa catgtggctc ccatggaccg
      961 gatccgcgag acggcaacac atattatcat gaagtcatac aaggaaaacg gtaacgtgct
     1021 ctttgaacaa caggaagcag aacgcctccc gtactcattt taaggtgagg gtatgcggaa
     1081 ttttatttct gaacgtgcac acaacatccc gccgtcgggg atcagaaaat tcttcgacct
     1141 tgctctcacg atagaggatg tcatatcgct tggtgttggc gaacccgatt ttcgtacccc
     1201 ctggaatatc tgtgagtcaa gcatttactc aattgaacag ggtgttacct cctacacccc
     1261 caacaaaggt ctgccagtgc tccgcgaaag cctctcgaat tacctgcagc agcactatca
     1321 ccttccttac aacagcgata atgaaattat tattacaagt ggtgtttccg aagggcttga
     1381 tattgcgatc cgggcaattg ttgaccctgg tgatgaagta cttattgctc agcccagtta
     1441 tgtctcttat gcgccgtgtg tgacgctggc aggaggaatt cctgttcccg ttcaatgtac
     1501 ggagcccgac cggttcaaac tcaacgcgga cgctctccag gataagatta ccggtaaaac
     1561 aaaagccctg atgatcaatt tcccgaacaa cccgacaggc gcagttatga accgggaaga
     1621 cctcagggca atttctgata ttatcatcga taaggatctc ctgctcatca cggatgaggt
     1681 atatgcagaa ctcacgtatg agggaagcca tgttgctgca gctacggtca gcgatctctg
     1741 gcagcggaca attacgctca atggtttctc aaaagcgtat gcaatgaccg gatggcgagt
     1801 cggatatctc tgtgctccaa aagagctctg tgaagcagtc ctgaagattc accagtatgt
     1861 gatgctatgc gcacctgtaa tgggacaagt cggggctctt gaagcgatcc gatctgcaga
     1921 agaggataaa aacaatatgg tgcaggagta ccgtttaaga cgcaacatgt ttattgcagg
     1981 tttaaaccgc atcggtcttc cggcacacgt tccagacggg gcattctatg cttttccctc
     2041 gataaaagaa accggacttt cggatacgga gtttgctgag cgactgttaa aagagcagag
     2101 ggttgcggtc gttcccggga gtgtctttgg agcaggggga gaggatcatg tacggtgcgc
     2161 atatgcggta tcccggaagc agcttacaga ggcacttggc cggattgaaa cattcctcac
     2221 gaatctcaga aattgatttc agaaacccca tttaaatttt tccaactttt agtcttcaag
     2281 tcaatcttta tgagcatact ttattttgca actcgatcaa tactacttga tattacaatg
     2341 agctgcacga tcatcgttgg tggatttttt ggagatgaag gtaaaggcaa gattgttgcc
     2401 catattgcat acaaggacaa acccgttatc atctcccgcg gcggtgtcgg gccgaatgcc
     2461 ggacacactg tacaggtagg ggaacgcgaa tatggtgtcc gtatggttcc ttcaggtttt
     2521 gtgtacaagg aagccaaact ctgtatcgga agcggggttc ttgttgatcc ccgcatcctg
     2581 aagcaggaag tcgaaatgct cgacgttaaa ggcagagttt ttgtcgataa acgctgtgga
     2641 attataactg aagaccacat cgtcagggat aaggggagtg cacacctttc aaagaagatc
     2701 ggaagtacag gatcaggatg tgggccggca aattctgaca gggtaatgag gctctctcct
     2761 caggcaaagg atgtaccaga acttgcggaa tatctgcttg atgttcccaa agccattgat
     2821 gatgaattaa aacagggcag gaccgttctg cttgaaggaa cgcagggttt tggcatctca
     2881 ctttattatg gcacctatcc ctttgtcacg agtaaagata catcagcatc ccagattgca
     2941 gcggataatg gggtaggtcc gacgaaaatt gatgacgtga tcgtggtttt caaggcatat
     3001 ccaacgcggg ttggtgaagg cccgttcagc accgaaatga catttgaaaa atctgattca
     3061 atgggaattc aggaatttgg gaccgtaaca caccggaagc gacgtattgg tgggtgggac
     3121 gggaatatgg cccggtattc agcaatgatc aatggatgta cccaagctgc gattaccggt
     3181 attgacaggg tggataaaag ctgttttgga gtaacggaat acgggaagct gacaaagaag
     3241 gcaaaggact ttttaaagag tgcagaggat gatattggct gttccattac tctcatatcc
     3301 accggaccag aaatgaccca gattattgat ttgagggaaa aacaggaatg agacgttcac
     3361 tcatggacat cctctgttgt ccggtttgca agggagatct ttctcttaac gtcgagaagg
     3421 aggatgaaaa ggaaattatc gagggtgagt tgaactgcgc ggtatgcaag gtaaattatc
     3481 ctattcatga gggtatcccg aatcttcttc ctcaaacatc ccagaaaaaa tgatagacag
     3541 gagcaggaat gaattttgca tctgcattca acattctgac tgttcaggat cccggattac
     3601 acgaaacatc ttttgaattt tatcttaacc ggttaggtat taatttaatt gagattccaa
     3661 gggagaatgt ctgggcagag attggtggca ccctgcgatt aatttttaaa aatcggggag
     3721 caccaaccca tctcacaatc tcctcaccta atgcagaaat gtttaccgat tttttccatg
     3781 agaatatgta tatcgttgat gaaaccgttc tgaccattcc aattagaaaa gactgtcagg
     3841 aaggtttttt cattcttgag attatcaccg gatacggcgg aatcaaaacc tctctccgtg
     3901 ttgatgtcat accgggagta gttattcaga aatctgctgt aaagcaagaa cccccacttc
     3961 aacctgttgc tcatggtcgc ccgcatctcc tgatggttat gatgggaatt gcactgattc
     4021 tttatagtgc attcctttat acaaaaaatg aatttctcaa caccgctgca tttatcatac
     4081 tcattgttgg tgctctctac acatggtacc gtcagcagta gtggctggag tagttgtact
     4141 atttgcggca cttattgttg atcgcctgat cggtgatccc caatcgccat ttcacccggt
     4201 tgctcttctt gggtggttta tagggaagtg gggtcagcca cgaaagtact cgccacaaat
     4261 gcagcgtttt gctggtgtac ttctctggat ggttacggca gccctgttcg cactgccatt
     4321 ttattatttt gcggcagttg ctccatggtt tctgtacctg gtcggtgccc cgattctgct
     4381 caaatgctgt tttgcatggc gatcgctgga agaacatact cttgcagtag tgcatgcact
     4441 gcatgcggga gtggaagaag gcagggatca ggtcaaaatg ctcgtatccc gggatactac
     4501 ccatcttaac cgggcacata ttctttcagc aggctatgaa tcgatgactg aaaatcttac
     4561 cgatagcatt atttcacccc ttttttattt ttccttattc ggtcttgtgg gcgcggccgt
     4621 ttatcgtgca gcaaacacca tggatgcgat gctcggatat aaggacgaga ggaaacgatt
     4681 aggctggttt tcggcacgaa tggatgacat ccttaatttt gttcctgccc ggctgaccgt
     4741 aatggtgctg cttgtgtatt ttgctttcac cggaaggttt tcacatgctg tgcgtgttat
     4801 gcgccgtgac ggttgcaaac gtcctggttt taatggagga attgtcatgg cagcgatggc
     4861 gggcggaaca ggaatacaat ttgtaaaacc aggggtatac gaaatcgggg ataatgaaag
     4921 aacatttgat gacgctggtg ctgaaattat ccgggcgggg cgattcgcaa ctattgcgtt
     4981 tacggtgctt gcgagtggta ctctgttttt attgggatca ttgatcaata gtaccggcat
     5041 atgaaacttg aagaactgag atttggtact gagcttctca agcgcggctt tgcatccatg
     5101 cagaaaggag gtgtgatcat ggatgtggtc aatgccgatc aggcaaagat tgcagaagaa
     5161 gctggtgccg tcgcagttat gtcactggaa cgtgtgccgt ctgatattcg gaaaatgggt
     5221 ggcgtcgccc gcatggcgga tccggaaaag gttattgaga ttattgatgc cgtttccatt
     5281 cctgtaatgg gcaaagtgcg tatcgggcat tttgtagaag ctcaggtttt ggaaacgctc
     5341 ggtgtagata tgattgatga gagtgaggtt ctgacaccag ccgatgaaca ataccatatt
     5401 gataaaaaac ttttcaagat cccctttgtc tgcggagccc gggatctggg agaggctctc
     5461 aggaggatcg atgaaggggc ggcaatgatc cggaccaaag gtgaggccgg gaccggaaat
     5521 gttgttgagg ctgtccggca catgcgcaat ataatgggag aaatccgttt actcaaaggt
     5581 atggatcgcc aggagatgat cgactatgca cgggaaattg aagcacccgc agaactggtt
     5641 attgagtgcg ccggccgtgg aagactcccg gttgtgaact tctcagcagg cggtatcgcc
     5701 actccttccg atgcggcaat gatgatgcag ctcggtgctg atggcgtttt tgttggatcc
     5761 gggattttca aatcctcaga tcctgaaaga atggcaaaag ctattgttga agcggttaat
     5821 catttcagcg atgcaacggt tatcgccaag gtaagcaagg gtcttggaga tgcgatgccc
     5881 ggccttgatg tgcacacact cagagaagat gaaaagctgc agactcgtgg acgttaagat
     5941 cggcgtgctc gcgctccagg gaaatgtgag cgaacacatc gatgcttttc ggttggcgct
     6001 catcagaatg gggcatacta cctcatctga agtttttgag gtcaggaatc ccgaagattt
     6061 ggcaggttgc gacgctctcg ctattcccgg tggcgaatct acaaccatct cccggctcat
     6121 tgataagaat cagatgtacg agccaatccg ctcgttttcc ggcggtatat ttgctacctg
     6181 tgctggcatg gtactggtgg cgacccatgt tgatgatgaa cgcatccatt cgctgggtct
     6241 tatcgaaata acggtggata ggaatgcttt cgggagacag cgggaatcgt tcgaagccga
     6301 tctctccatc gaatgtcttg aaggaggacc attccacgcg attttcatcc gtgcgcctgt
     6361 tgcaacatcc gctggccctg gtgtaacggt gctttcatgt atggatcagg gaatagttgc
     6421 agtagaatat ggaaagcata tagcactctc ttttcacccg gaactttcgt gtgatacccg
     6481 gttgcatgaa cgatttttaa gggaactagg ggtttgatgt ttatcagatt gaaagcaatc
     6541 ttcattgtcg aatatcagat ggatgatgcg ttacttacct gaaagacaat gaaatgaact
     6601 tggaagatga aaaatcgaaa aatgagttat ctatggacga atggtcctat ctatagtctt
     6661 accaatcctt tttcatcgct tcagtttttt ggtcctttgg caaaagctca atagcatatc
     6721 tcaatgctgt cctgggcatg gatttcctgt ttcgaaggac aaaatcaaag acttctgtcc
     6781 cgtgttttcg gctggcttct tttaagagcc acccatatcc cttctgaacc atgtcatcac
     6841 tatcagtgag cagaaggtct gcaatctcca tagactcata gaggaatttc ccgcgttttg
     6901 caggtataat aagggatact gccgatgcac gccgcatcca gcggttcggt gactttgtcc
     6961 agcattttag ctctttgata tactccgggt attgttcaat gaagttgccc attgtatggt
     7021 tgcaaaaacc atcacaggat gcccagttgg tgatatatga atcaatccag tttctgaaga
     7081 ccgacaggtc atcaggctca tagtgcccta taagggagct tgtccagtta gaaacgatga
     7141 atgactcctc aaggtaaccg gatttgtata aatcctcgca aagaaagaag atctccggct
     7201 tgggcctgca ccgaaccacc ttccagtact tttttgcaat cgcaagtacg gtggcagttt
     7261 tgattccata acacgctatc tcttccttga agaaacgctg ggaggttttc cggatctcca
     7321 tgtcctgatg gtctttcagt tcctggcgga tttgggttat taccggatcc atggaaaaat
     7381 attgtgtcgg aggggaataa ggatgcggca tcctttgccc ccgaattacc gcgacgtccc
     7441 gttgttgaca ttaaagcccc atcttttgcg gcagattccg tgcccatgca gcaatcgcaa
     7501 tccagtcgcg aaaatcgccg gtgggaactt tcataagcga tgtcaggcat cgctctatga
     7561 agttcatttt tcctgcatca agagtaccag caaacaccgt cactgagacc ggacttgacg
     7621 gagctaaagc cacaatcagc tgatcagtga actcttttac ctctccggtc ttaggataca
     7681 ccggtgcaat accagtcaca aatccggcca ccggtaaacg tgatatcgcg tctttatgag
     7741 ctgctgcgaa tgcaacaaca tcacctttta cctttcccgt gtaaaatggt gctcctatca
     7801 caaccgcact gaatccaaca agtgatgtga ctgatttcat ctcgctgata tcgacagcat
     7861 atcctttgga aattagttcc ttgccaatcg catgagcaat cccggcagtc gatcccttcc
     7921 gcgatgcata tgcaacaaga attcgtgcag acataatgat ccctgcctga atggttggat
     7981 gcatcactta taaaaacagg gcaaagtgtc ccgttcaaca accatcagtt cactgcctgg
     8041 cttaccgctg tttcaacagc tatttggaac ccactgaggt gtttcctctc cctctcatca
     8101 tgcagggcgt tgagacaaac ttcgcaatgg taacgttgaa tccaaactct tgctctttct
     8161 gcatcttcta cggttacgtt ccacatttca tgaatcttca tattgtgcta ccaggtgaag
     8221 ataaatctaa aggaaacaac cacgattcct gtagaggtat tagggtgcac ataatggcta
     8281 ccgatcctgt ctgttttgca gttgttgatg gtgctcgttt cacgaggata tacaaaaatc
     8341 agaagtgtta cttccgcacg aactacggta aaaacaagta tgatgaagtc ctcaggatat
     8401 tcagggatgg cttgtgatct taatattgaa caccgtgggg catcgaacta aaaacaacca
     8461 aatgttatgg gataaaaggc aataccactt aatgaaatac acccgatact aaatagaggc
     8521 taatgaggaa accatgccaa ccgatccagt ctgttttatg atagttgatg aagatgacgc
     8581 taaattcact agtaaataca aggatcacga atattatttc tgctgtaact ggtgtaaaaa
     8641 aaagtttgat gagaacccga agaggtattg gcgtagttct tccgatatca gtgtcaatct
     8701 tagtgatgtt acctgagcgg taataaccgg gcatgtatcc gtcatcgcaa tgctggtcgg
     8761 ttatccgaaa accccgttgg aaataccttg ggattgctga gagctgatat aggtttttaa
     8821 tcgggtgggc agatacagat atatcaatcc atagatcaaa acgttcaata ctctatttat
     8881 atgaaacccg atgatgaccc ctggctcaaa aacgcacata aaaatgcaga acttctcgac
     8941 tgtgagaaca aaaaactttt gttattaata aatccaatac ggaaaaacct tcctctcatt
     9001 tccaaatatc aggatttctg gaatcaggca aaactgatct ccgcgttatt caaacaac
//
LOCUS       DASRLV010000075         3876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_510250,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000075 DASRLV010000000
VERSION     DASRLV010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510250"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VFC43_03115"
     CDS             <1..669
                     /locus_tag="VFC43_03115"
                     /inference="COORDINATES: protein motif:HMM:NF036560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas6"
                     /protein_id="HZK30209.1"
                     /translation="PVFTLVLQPANPLHFSLYELRTFFTRQLTNHARLHLHSSAAGIH
                     RYPVIQCKQVKNRLLVIGITQGADLLLDLSLGTQELLLGENTSSIIERDQEIRNETVG
                     ISETLQRYEFLTPWLALNQQNAKKFYDLKGKPERDAFMQKILTGSISTFAKSIDCDLR
                     APLMCRSKVRFRRERIHDENVMVFLGTFATNLRIPDYLGIGQSISQGFGTIRSVADDP
                     GTLS"
     gene            682..1251
                     /locus_tag="VFC43_03120"
     CDS             682..1251
                     /locus_tag="VFC43_03120"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HZK30210.1"
                     /translation="MNTALLVIDVQNEYFSGSMEVTYPKESLKNILLVMDHAHAKGIP
                     VAVIRHTNLAGTAGTFKKGTMNWELHDEIKQRPADIHVEKNLPGSFTRTTLDTWLKSK
                     GISRVTIAGYMTQMCCDTTARQAFHRGYAVDFLSDATGTLSVTNSAGTITASDLHKAV
                     LITQQMLFSRVLSTDEWIQKVTGEQKLSS"
     gene            1331..2260
                     /locus_tag="VFC43_03125"
     CDS             1331..2260
                     /locus_tag="VFC43_03125"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZK30211.1"
                     /translation="MLWIFLALLGAITNAGYFIIIKRSIASLDPNVLTGIGFTCGGLI
                     LFTISAIRTFPVIGEDLYSAVAITAILNIISLSLIFKSLSSSDLSLCIPMLSFTPVIL
                     TGTSFLLLHEVPSFFGFVGICIIVSGSYVLNISEGDMHFLDPVKTILRNRGSWYMLIV
                     ALLFAVSINYDKIALLNSDPFFGMALTVLSIGVSFVLISAYSRISVQGKSFKKPAERK
                     EPVSVPESRKSRLKKYLIISFIIGAFVATEAASVNLAYTLQIVPYVIAIKRLSIIFVV
                     IYGTMVFSEHEISKRLAGAVLMVMGAIIIMMFS"
     gene            complement(2272..>3876)
                     /gene="ade"
                     /locus_tag="VFC43_03130"
     CDS             complement(2272..>3876)
                     /gene="ade"
                     /locus_tag="VFC43_03130"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HZK30212.1"
                     /translation="KNASVFNPFTCDWEQGTLAVKNGLVLGIGSYTGKTEFDVEGLYI
                     VPGFIDAHVHIESSLLVPREYARLVVRHGTTSVIADPHEIANVSGTAGIDFMLAGRQG
                     AAMDILYTLPSCVPATPLSRGGAVLDSRDLLPYAGREGIVGLGEMMNVPGVLAADSGI
                     LEKLQLLPLRDGHAPLLAGKDLNAYILAGLQSDHECTSLAEAEEKLRRGMYIFIREGS
                     TERNIRDLIGLVTPQTVSRCCFASDDCHADTLFEKGHIDHCVRRAVDCGLEPELAYRM
                     ATLSPAERFGLLDRGALTPGRRADFCIIDDPLTCRVKKVFVKGNEVLPDQRPVRMVDL
                     PGTFRCVIPNPEAIRISGSGEARVIGLIPHQIMTKSLRYTIGVHEIPDFTKDILKIVV
                     CNRYGNNACGVGLVHGFSFKQGAIASSVSHDAHNIIAVGTSDEEIRRAIEAVILSKGA
                     MVAVFGESVTTLPLPCGGLMSTLPYPEVVERLGNLHTVSNAMGCIDNPFMYLSFLALP
                     VIPSLRITDKGMFDVLKNTHVPIFTG"
BASE COUNT         1001 a          979 c          902 g          994 t
ORIGIN      
        1 ccggtcttca cgctcgtcct tcaaccagca aatccgcttc atttttcctt atatgagctt
       61 cgcacttttt tcacacgaca attaaccaac cacgccagac ttcacctgca ttcctccgca
      121 gccggcattc accgctatcc ggtcatccag tgcaaacaag ttaaaaacag gctcctggta
      181 atcggtatca ctcagggcgc agatctcctt ttggaccttt ctcttggcac tcaggagtta
      241 ctattgggtg aaaacaccag ttccattatt gaacgggatc aggagatcag gaatgaaaca
      301 gtgggcattt cagaaaccct tcaaagatat gaattcctga ccccatggct cgcgctgaac
      361 cagcagaatg caaaaaaatt ctatgacctg aagggaaaac cggagagaga tgcattcatg
      421 caaaaaattc tcaccggcag catcagcacc tttgccaaat ccattgactg tgatctgcgg
      481 gcaccgctca tgtgcaggag caaagtgcgg ttcagacggg aaaggattca tgatgaaaac
      541 gtaatggtgt tccttggaac gttcgcaaca aatctccgga taccggatta tctgggaatc
      601 gggcagtcga tttcacaggg atttggcact atacgatctg tcgctgatga tccaggaact
      661 ttatcataag gagagattgc catgaatact gctttgcttg ttattgatgt tcagaatgaa
      721 tatttctccg gctcaatgga ggtgacatac ccaaaagaaa gtttaaaaaa tattcttttg
      781 gtaatggatc atgcacatgc caaaggcatt cctgttgcgg taatccggca taccaatctg
      841 gcaggaactg caggcacatt caaaaaaggg acgatgaatt gggaacttca tgatgagatc
      901 aaacaacgcc ctgcagatat tcatgtggaa aagaacctgc cggggagttt tacccgtact
      961 actctcgaca cctggctgaa gagtaagggg atttcaaggg tcaccattgc cggttacatg
     1021 acccagatgt gctgcgatac cactgcccga caggcatttc accggggata tgcggttgat
     1081 ttcctgtccg atgctacagg aaccctttca gtcaccaatt ctgccggtac aattactgca
     1141 agtgatcttc acaaagcagt cctgataacc cagcagatgt tgttctcccg ggttctctcg
     1201 accgatgaat ggatacaaaa agtgaccggg gaacaaaaac tctcatcata acaattttct
     1261 ctgtttccca aaaaaaaaga aaaagcccga tatgcgataa catacaaaca gtactgcgga
     1321 tctcgtaggt atgctctgga tttttctcgc cctcctcggc gctattacca atgccggcta
     1381 ttttattatc attaaacgct ctattgcgtc gctggatcca aacgtcctga ctggcatcgg
     1441 ttttacctgc gggggactta tactttttac catttctgcg atccggacat tcccggtcat
     1501 cggtgaggat ttgtattccg cggttgcgat aaccgccatc ctgaatatta tcagtctctc
     1561 gctgatcttc aaatcactgt cctcttcgga tctctccctg tgcatcccca tgctctcgtt
     1621 cacaccggta atattgaccg gtacatcctt tctcctctta cacgaagtcc cctcattctt
     1681 cgggtttgtt ggcatctgta tcatcgtgag cggatcgtat gtcctgaata tttctgaggg
     1741 ggatatgcat tttttggacc cggtaaagac aatcctccgg aaccggggga gctggtatat
     1801 gctgattgtt gcactgctgt ttgcggtgtc catcaactat gataaaatag cgctcctcaa
     1861 ttctgaccct ttcttcggta tggccctgac ggtcctttca atcggtgtat cgtttgtcct
     1921 gatatctgca tattcccgta tatctgttca gggaaaatct tttaagaaac ccgctgaaag
     1981 gaaagaacca gtctccgttc cagaatccag gaaatcccgc ctgaaaaaat atttgattat
     2041 ttcctttatt atcggcgcat ttgtagccac ggaagcagca tcagtaaatc ttgcatatac
     2101 acttcaaatc gtaccatacg tcatcgcgat aaaacgtttg agtattatct ttgtcgtaat
     2161 ctacggcacc atggtgtttt ctgaacatga aatcagcaaa aggctggcag gagcagtact
     2221 gatggttatg ggtgcaataa ttattatgat gttttcataa gaagtttttt tttacccggt
     2281 gaagatgggg acatgggtat ttttcaaaac atcgaacatt cccttgtctg tgatcctcag
     2341 tgacgggatc actggcagag caagaaacga gaggtacata aagggattat caatgcagcc
     2401 catcgcgttg ctaacggtat gaagatttcc gagccgttct acgacttccg gatacggtaa
     2461 cgtcgacatc aaacccccgc acgggagtgg gagggtggtt accgattctc caaaaacagc
     2521 gaccatggct cctttggaca ggataacagc ttcaatcgcc cttcggattt cttcatcgct
     2581 tgtccccacc gcaatgatat tatgggcatc atgggaaaca ctggacgcaa tcgctccctg
     2641 tttgaaagag aaaccgtgaa caagcccgac accacacgca ttatttccgt accggttgca
     2701 gacaactatt ttcaggatgt ctttggtaaa atcaggtatt tcgtgtaccc cgatagtgta
     2761 acgcaatgat tttgtcatga tctggtgcgg tatgagcccg attacccggg cttctccact
     2821 cccggaaata cggattgctt caggattggg gatcacgcac ctgaatgttc ccgggagatc
     2881 caccatcctt accggtcgct ggtcaggcaa aacctcattc cccttcacaa agactttttt
     2941 gaccctgcac gtgagggggt catcgattat acagaaatct gcacgccggc cgggagtaag
     3001 tgccccccgg tcaagtagtc caaaccgttc tgcaggagaa agcgttgcca tccggtacgc
     3061 cagttcgggt tcaagtccgc agtctacagc ccgccgtacg caatgatcga tgtgtccttt
     3121 ttcaaagagg gtatcagcat ggcagtcatc agaggcaaaa caacaccgtg aaacggtttg
     3181 gggagtcaca aggccgataa ggtcacggat attgcgttcg gttgatcctt cccggatgaa
     3241 tatatacatt ccgcgacgga gtttttcttc tgcttccgca aggcttgtac attcatgatc
     3301 actttgcagg ccggcaagaa tgtatgcatt gaggtctttt ccggcaagca gcggggcatg
     3361 cccatcacga agaggcaaga gctgaagttt ttccaaaatc ccggaatcag cagcaaggac
     3421 gccggggacg ttcatcatct cgcccagccc cacgattcct tcacgaccgg catacggtaa
     3481 aaggtcccgg ctgtcaagga ctgccccgcc ccggctcagg ggtgttgccg ggacgcacga
     3541 cggaagggta tagaggatat ccatggccgc accctgccgc ccggcaagca tgaagtcaat
     3601 tcccgctgtg ccggagacat tcgcaatttc gtgcggatcg gcaattacgg aagttgtgcc
     3661 gtgacggact accaggcggg catactcgcg gggaacgagc agcgagctct cgatatggac
     3721 atgtgcatcg ataaaaccgg gaacaatgta caaaccctca acatcgaatt cagtttttcc
     3781 cgtatacgag ccaatcccga gaaccagccc gtttttcacc gcaagggtgc cctgttccca
     3841 gtcacacgta aaaggattga aaacagaagc gttttt
//
LOCUS       DASRLV010000076         5973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_757821,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000076 DASRLV010000000
VERSION     DASRLV010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5973
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757821"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="VFC43_03135"
     CDS             complement(<1..595)
                     /locus_tag="VFC43_03135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZK30213.1"
                     /translation="MINTTCLPSFDQVPFGVILLQKDLSVRFWNHILEDWTGIKKEDI
                     IGKNLIDQYPELNNPSVILRFPEVFEGRTPVIFSSHFHPHLIPSVLPDGSLRIQKGAV
                     TPICLNNEIHAIFVIEDITDLVRQVTAYRKLRDIARQELAERKKAEESLRIANTKLSL
                     FSDTTLLDIKSQIDISLGLVSFIENDLTNKEEIRANSS"
     gene            complement(620..1225)
                     /locus_tag="VFC43_03140"
     CDS             complement(620..1225)
                     /locus_tag="VFC43_03140"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HZK30214.1"
                     /translation="MDVDVESLDAITETINIGVGKSASLLNKMTGSHIRLQVPKLSVV
                     RFKDLEKTSWNLSADQKISSVLLEFQGNFSGVTAIIFPPESATSLVMLLSGENEPGPE
                     MDAIRMETLKEVGNIIINSIMGSISNILSQHLSFSIPVYYEGKLSGVRAIQRNINDDD
                     WVIVAYTQFQVEAKHIEGIILILLEVGSLEHLVGIIQGATR"
     gene            complement(1232..1594)
                     /locus_tag="VFC43_03145"
     CDS             complement(1232..1594)
                     /locus_tag="VFC43_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZK30215.1"
                     /translation="MAKILVIDDSSFQRKIISGVLTENGYQVTLAANGKEGIEHISRE
                     QPDIIITDLLMPEYDGYWVLEHLNAQKRTIPVIILTSDIQTTTEKRCRELGVTAFLNK
                     PVDKKQLISTVQNTLKRS"
     gene            2003..2608
                     /locus_tag="VFC43_03150"
     CDS             2003..2608
                     /locus_tag="VFC43_03150"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HZK30216.1"
                     /translation="MQNQHFPLNTRFKPSTAMMTWYRAGIALLFGLLLLFSYVPAVLF
                     GEMPAIFSVLILGSTALIFILLWIWVGKYYDSMFYELRDDEINWKRGVWFRTTGIVPY
                     NRITNLDVRQGPFMRMLKISNLAMQTAGYSGQAVPEIQIEAIEHAEELRELIRSLIRG
                     SSSHDDGTGSAKPSLSPTMKTTDQQILAEVQQIRSLLEQRR"
     gene            2841..3968
                     /locus_tag="VFC43_03155"
     CDS             2841..3968
                     /locus_tag="VFC43_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30217.1"
                     /translation="MKSGHTALIRRVVLQIFFVILLIAGTGMVLAHAPLGVGTNEDIA
                     NATLIGTPEKSFVLYTELHESGEAQYYLFPLLKGQRLYGSLQVPGPGSMIPDLVIIGP
                     GIEPAGTVPSSVQVPSGSGVMVIPGNPPKKPSYEPFSPQPIYEVARFNVTVPKDGNYY
                     IAVSGPEGAKYSLAPGFLEEFTAAEWLTIPLSVVSIHLWEGQSPAGIFAPLIIVMAVG
                     LGLIFLYPGWRAVRRDPIACLVILSGFLYLGGAAMTALQIVHAVQVTGYTSEVILTLL
                     FIAGPLILGVFAIRAGSRLPDVSYSWSDGIRMMGIGFLGLLVWAGLIIGPVLAILSGV
                     LILIKTAMKSGEATYKSLEGLNLKPQYFGKYPENNLMNNEN"
     gene            complement(4097..4555)
                     /locus_tag="VFC43_03160"
     CDS             complement(4097..4555)
                     /locus_tag="VFC43_03160"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZK30218.1"
                     /translation="MNILDAGCGPGRLTLPMAKKISPLGQVTAIDIQEGMLLEAQKRA
                     REAHLTNIRFLQLALGQGRIEHNYFDRAVLVTVLGEIPDREAALGEIFGSLKHGGILL
                     VEETILDPHFQTRGTVLKMAMATGFREKEFFGNRFSYTLTLQKPSASGQR"
     gene            complement(4568..4750)
                     /locus_tag="VFC43_03165"
     CDS             complement(4568..4750)
                     /locus_tag="VFC43_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30219.1"
                     /translation="MISFLHLFLIVIALVIIIALVWRLASRRYAIPCPVWMKGLLDPP
                     FNKGISQRNQKTIERL"
     gene            5106..5417
                     /locus_tag="VFC43_03170"
     CDS             5106..5417
                     /locus_tag="VFC43_03170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HZK30220.1"
                     /translation="MILVDAKCTIVPERHDDFIREVRDITPAVRKETGCIRYELVADV
                     QVPGVFHFIEEWESQKHLDDHIQKPHMRVYFAKTSPWHSAPAELKIYEILSSRLVMIN
                     D"
     gene            5594..>5973
                     /locus_tag="VFC43_03175"
     CDS             5594..>5973
                     /locus_tag="VFC43_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30221.1"
                     /translation="MKISLFVTLACAVLVLILAAGCTQQAVQPTPTFTQPPTTIMTPT
                     PTAVTTTLPTTTIPLPTMTAPAILLPKSIKDTPLLFTISAPDGYAGTTIRATTYEYNI
                     LYKTTIFNPANGDANMTINNNSGT"
BASE COUNT         1500 a         1491 c         1374 g         1608 t
ORIGIN      
        1 cggaggaatt tgcgcggatc tcttctttat tggtaagatc attttcaata aacgaaacaa
       61 gacccagcga tatgtcaatc tggctcttga tatcaagaag cgtggtgtcc gaaaacaggc
      121 tcagtttggt atttgctatc cgtaaggatt cctccgcttt cttacgctcg gcgagttcct
      181 gcctggcgat atccctcagc ttgcggtaag cggttacctg ccggacaaga tcggtaatgt
      241 cttcaatcac aaatattgca tgaatctcgt tattaaggca tatcggggta actgcacctt
      301 tctggatccg gagagacccg tcaggaagta cagaaggaat caggtgggga tggaaatggg
      361 aagaaaaaat aacgggtgtc cgtccttcga atacttcggg aaaccgaagg attacagaag
      421 ggttattgag ttcgggatac tggtcaatga gattttttcc tatgatatct tcttttttta
      481 tcccggtcca gtcctcaagg atatggttcc agaaccggac cgacagatct ttttgcagaa
      541 gaataacccc gaacgggacc tgatcaaacg agggaagaca agttgtattt atcatcacat
      601 ttttcatcgg tttttccccc tatctggtgg ctccctgtat gatcccgaca agatgctcca
      661 gggacccgac ttcgagcagg ataagaatta ttccttcgat gtgttttgct tctacctgga
      721 attgcgtata tgctacaatg acccagtcat catcattgat attgcgctgt atcgccctga
      781 ctcctgacaa ttttccttcg taatacaccg gaatggaaaa tgagagatgt tgagaaagaa
      841 tatttgaaat tgagcccatt attgagttga taatgatgtt cccgacttct ttgagagtct
      901 ccatccggat ggcatccatt tccggcccgg gttcattctc accggaaaga agcataacca
      961 gggacgtagc actctctgga ggaaaaatga ttgcggtgac accggaaaaa tttccctgga
     1021 actccagtaa aacacttgat atcttttgat cggccgagag attccagctg gttttttcca
     1081 ggtctttgaa ccggaccacg gaaagtttcg ggacctggag ccggatatga gaaccggtca
     1141 tcttgtttaa gagggatgcc gattttccca cgccaatgtt aattgtttct gttattgcat
     1201 caagcgattc aacatcgaca tccattgata atcacgacct cttgagcgta ttctggaccg
     1261 ttgagatcag ctgtttctta tccactggtt tattcaggaa tgcggtgacg ccaagttccc
     1321 tgcacctttt ttcggttgtc gtctggatgt cggaggtaag gatgataacc ggaatagtgc
     1381 gtttctgggc attcagatgt tcaagtaccc agtacccgtc atattcgggc atgagaagat
     1441 ccgtgatgat gatatccggt tgttccctgc ttatgtgttc aatgccctct tttccgtttg
     1501 cagcaagagt aacctgatat ccgttttccg tcaggacacc cgaaatgatc tttcgctgaa
     1561 atgatgaatc atcaatcaca agaattttcg ccaaagtaaa atcacccttt caatacaata
     1621 tcagggatta tccttattaa acggtgtgat tcccagtaat gcgacgttct ctctctcccc
     1681 gctcctgtaa agatcaggac tatctttcat aacatggaaa gaatatccgc tgaatacctt
     1741 ctgggaggtg ctccggatag cgatctcagg tcaagactat gcccttatta ttttagaaac
     1801 ggacatgcat gacatcaggc aggtcgtcga ggaaagagtg ctgaagtggc ctgttggagc
     1861 aaagaaaatg atgttttggc tttcccgcgt aggtatcgtt gaaaaaccta ctttgcaatg
     1921 aaggtgaccg ggggaatttt cctgacccgg ttttcccgta atccttatgt tcttttctgg
     1981 tttactacat acgggaatta tcatgcaaaa ccagcatttt cctctcaaca cgcgatttaa
     2041 accatcgacc gcgatgatga cctggtacag ggcaggaatc gccctgctct tcggtttact
     2101 gcttctcttc tcctacgtac ctgcggtact ctttggtgag atgcccgcga ttttctctgt
     2161 tctcatcctt ggttcaaccg cactgatatt tatccttctg tggatctggg tcggcaaata
     2221 ttacgacagc atgttctacg aactgcgcga tgatgagata aactggaaga gaggagtctg
     2281 gttccggacc accggtatag tcccctataa ccggatcacc aatctcgatg tccgtcaggg
     2341 gccttttatg cgtatgctca aaatttcaaa cctcgcgatg cagaccgccg gatattcagg
     2401 gcaggcagtg cccgagatcc agatagaagc gattgaacat gctgaagaac ttcgcgaact
     2461 gatccgctcc ctgatacggg gatcttcctc gcatgatgac ggtaccggaa gtgcaaaacc
     2521 ttctctttct cccaccatga aaacgacaga ccagcagatc ctcgcagaag tacaacaaat
     2581 ccggtccctg ctcgagcagc gacggtgaca gtaatttccc ctttttgtcc ctgaaccctg
     2641 aaaaagggat agtgtctcaa cggtgcatga atccaaccgc agtgctgtca ccatcccgtg
     2701 ggatgatctc gttatctccc gattgcagat tccattctta caaggatcca gttttctcca
     2761 aagaccatgt agggtatttt atgcctttcc tggaggtatc ccaccgaaac acacaaataa
     2821 tccgcaaatg atctctgcac atgaaatccg ggcacacggc tttaataaga agagttgttc
     2881 ttcagatttt ttttgttatt ctcctcattg caggaaccgg tatggttttg gcacatgcac
     2941 cgctgggggt tggtaccaat gaggatattg cgaatgcgac cctgatcggc actcccgaaa
     3001 aatcttttgt actgtataca gagctccatg agagcgggga agcccagtac tatcttttcc
     3061 ccctgctgaa agggcagcgt ctctatggtt ccctgcaggt gccgggtcca ggttcgatga
     3121 tcccggacct cgtcatcatc gggcccggca ttgaaccggc aggaactgtc ccgtcgtctg
     3181 tccaggtacc ttctggttca ggggtaatgg taattccggg caatcccccg aaaaaacctt
     3241 cctacgagcc tttttccccc cagccaatct atgaggtagc gcgattcaat gtaaccgtcc
     3301 caaaggatgg gaattattat attgcagtat ctggacctga aggggcgaaa tacagtcttg
     3361 cacccgggtt cttagaggaa ttcaccgcgg ccgaatggct gacgataccc ctttcagttg
     3421 tctctatcca tctctgggaa ggccagtcgc ctgccgggat atttgcaccg ttgattatcg
     3481 ttatggctgt gggcctgggg ctcatattcc tgtacccggg ctggagagct gtgcgacggg
     3541 atccgattgc ctgcctggtg atcctgtcag gattcctgta ccttggcggt gcagccatga
     3601 cagccctgca gattgttcat gcagtccagg taacgggtta tacctctgaa gttatcctga
     3661 ccctcctgtt cattgcaggt ccgctcatcc ttggcgtttt cgcaatccgc gccggaagca
     3721 ggttaccgga cgtgtcttat tcctggtcgg acgggatcag gatgatgggt attggattcc
     3781 tgggtcttct ggtctgggcc ggtcttatta tcggacctgt tcttgcgatt ctcagcggtg
     3841 tcctgatcct gataaaaact gcaatgaagt ctggtgaagc cacatacaag tcattagaag
     3901 ggttgaatct caaaccacaa tatttcggga aataccctga aaacaacctc atgaacaatg
     3961 aaaattgaat gaattttcca ttacgctccc ttgttttttt aaaaattgca ggaaaattcc
     4021 ccggcactga aaaaactccg tatttgtctt atacaggatc ttccctgctt tttttatctt
     4081 caaacccttc agaagttcag cgttgcccgg aagcagaggg tttttgcagc gtaagcgtat
     4141 aggaaaaacg gttcccgaaa aattcctttt ccctaaaccc tgttgccatt gccatcttga
     4201 ggacagtacc gcgggtctgg aaatgcgggt ccaggattgt ctcttcgacc agcaggatac
     4261 ccccgtgctt cagcgatcca aaaatttccc ccaacgctgc ttcccgatca gggatctctc
     4321 ctaataccgt aacaagcacg gcccggtcaa aataattgtg ctcaatcctg ccttgcccga
     4381 gtgcgagctg aagaaaacga atattggtga gatgtgcctc gcgggcccgt ttctgcgcct
     4441 caagcagcat cccttcctga atatctattg cggtgacttg gcctaatggg ctgatcttct
     4501 ttgccatggg cagcgttagt cttccgggac cacaccctgc gtcgaggata ttcatgccgg
     4561 gactgagcta aagccgctcg attgtctttt gattccgctg gctgatgccc ttgttaaacg
     4621 ggggatcaag cagtcccttc atccatacgg ggcagggaat cgcataccgc cgtgacgcaa
     4681 gccgccatac gagggcaatg atgatgacca gcgcaatgac gatgaggaag aggtgtaaaa
     4741 atgagatcat gggcgtcctg tttatctaat aaaagatctt ttgacacata ttttttttta
     4801 cctgagcggc tgaaaggcat tgcagagact gaaaaacaaa agaaaggact ctttttgtga
     4861 tcctagagaa acacaaaaaa atttttgacc gatcaggtca gcgattccaa ttttacagat
     4921 ttttgccttt ttttcctgta atcaggactc cctgtttccg cggaatgaat aaaaaccgct
     4981 gatctgatat ccgacaggag aaataattac gtgagcggcc ccattgaata cacggtgttc
     5041 cttaaaaacc aggctgaacc tgttaaaccc ttgccagggt ctgatcagtg ggggagcaaa
     5101 catatatgat acttgttgat gcgaagtgta ctattgtgcc ggaacggcac gatgatttta
     5161 tcagggaagt ccgggatatc accccggcag tccgtaagga aacaggatgc atccggtacg
     5221 aactggttgc tgatgtccag gttcccgggg tatttcattt cattgaagaa tgggaaagtc
     5281 agaaacatct ggatgaccat atccaaaaac cccatatgcg ggtatatttt gcaaaaactt
     5341 ccccatggca ttcggcccct gctgaattga agatctacga gatcctgtca tcgcgattgg
     5401 tgatgatcaa tgactgatga cggcatgcaa gaccctgaaa aaagccggtc cccctcaggc
     5461 tctggtcgat gaacaaacag gtatccacag ttttattctc cgaaaatctc ctctacatat
     5521 tcgttctttc cttttcgttt ccaaagttgt aaaataccga agggtataac aaccgtcctg
     5581 catatcagag tgtatgaaaa tttcattgtt tgtaaccctt gcctgtgcgg ttctcgtcct
     5641 gattttagcc gccgggtgca cacagcaggc tgtccagcct acccccacat ttacccaacc
     5701 cccgacaacc attatgaccc ctactccgac tgcggtaacc accaccctcc ccacaaccac
     5761 aattcccctg ccaactatga ccgcacccgc aattcttctt ccaaaaagca tcaaggacac
     5821 accgcttctc ttcaccatct ctgcacctga cgggtatgcg ggcacaacca tccgggcaac
     5881 aacatatgag tacaacatcc tctataagac gaccatcttc aacccggcca atggtgacgc
     5941 caacatgacc ataaacaaca acagtggcac gta
//
LOCUS       DASRLV010000077         2282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_997271,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000077 DASRLV010000000
VERSION     DASRLV010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997271"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VFC43_03180"
     CDS             <1..224
                     /locus_tag="VFC43_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30222.1"
                     /translation="LTETMKRDVPSGTRPQRYTQILLFDNDSTTKTAVQHRVLDELAC
                     IKPDEMTPLQALAKIAELRNMLENREVSP"
     gene            221..2020
                     /gene="mutL"
                     /locus_tag="VFC43_03185"
     CDS             221..2020
                     /gene="mutL"
                     /locus_tag="VFC43_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HZK30223.1"
                     /translation="MKNVNTVSAIHILDQATVCQIAAGEVVERLASVVKELVENAIDA
                     GARSIRIDITSSVKEILSIRITDDGSGMSPSDAVLAFTPHATSKMTRIDDLHRIHTLG
                     FRGEALASIAAVSRVTLKTRLRNTGQGTGTKVLVEGGKIQETGETGAPEGTNVLVEDI
                     FFNTPARKKFQRSKNTEIAHIHAILEGICLAHPEISFRLYTDKNEQLVTDRSPRILDT
                     IARIYGSDVVKDLIMVNWSLPFMKISGYISRPSLSRRDASRILIAINQRYVSSTIING
                     AIKEGYGTLLPKDRFPIAFLTLVIDTGLVDVNVHPTKKLVRLSREKEIVRAMSEAVKT
                     ALLSLDLIPDVDVSPQIFLEDAGKEAVTVPNPVYDFTGPVPSGIRDSLHSGTIMTDRQ
                     LRQTELPTGMVPMDAKLPVMEVIGQLGGIYILATTESGELVLIDQHAIHERILYEQVI
                     ARTAGEHRSQELIVPFILHRTLQETAILRDLLPALAQEGFVLEEFGRDTFLVRAIPVV
                     LGKLEDTSIIDEMVSDLVSEEPVRSVNTRERITRIIACRGAIKAGTVCTQEQSQRLIQ
                     QIRYAKNPFTCPHGRPTMLRFSRTELDIMFKRS"
BASE COUNT          618 a          570 c          552 g          542 t
ORIGIN      
        1 ttcttaccga gaccatgaaa cgcgatgtcc cttccggcac ccgtccccag cgttacaccc
       61 agatactttt attcgataat gacagcacaa caaaaaccgc tgtacagcac cgtgtccttg
      121 atgaacttgc atgtatcaaa ccggatgaga tgacacccct gcaggcactt gcaaagattg
      181 ccgagttacg taatatgctg gaaaataggg aagtatcccc atgaagaatg tgaatactgt
      241 ttccgctatc cacatccttg atcaggcaac cgtctgccag attgcagcag gcgaggtggt
      301 cgagcgcctg gcatccgtgg taaaagagct cgtggaaaat gcgatcgatg ccggtgcccg
      361 ttcaatccgc atcgatatta cctcttctgt taaagaaatt ctgagtatcc gtatcacgga
      421 cgacggatcc ggtatgtctc cttccgatgc tgtgctcgcc ttcacccccc atgcaacgag
      481 taagatgacc cggatcgacg atctccatcg tatccacact ctcggattcc gcggagaagc
      541 tctcgccagc attgcggcgg tgtccagggt caccctgaaa acacgacttc ggaatacagg
      601 gcaaggtacc gggacgaagg tgcttgttga aggtggaaaa atccaggaga caggagaaac
      661 cggggcacct gaaggaacga atgtactggt agaagacatt ttcttcaaca caccggcacg
      721 aaaaaagttt cagaggagta aaaatacaga gatcgcacat atccatgcga ttctggaagg
      781 catctgcctt gctcatcccg aaatatcatt ccgtttgtat actgataaaa atgagcagct
      841 tgtgacggat cgatcacccc ggatccttga taccattgcc cgcatctatg gcagcgatgt
      901 ggttaaggat ctaataatgg tgaattggtc tcttccgttc atgaagattt ccgggtacat
      961 atcacgccca tctctaagcc ggagagacgc atcacggata ctgatagcga tcaatcaacg
     1021 atatgtatct tccacaataa taaacggggc catcaaagag ggatatggca ccctgctacc
     1081 aaaagaccgg ttcccgattg cattcctcac tcttgtgatt gatacagggc ttgtcgacgt
     1141 aaatgtgcac ccgacgaaaa aactggtgcg actctcccgg gaaaaagaga ttgtccgggc
     1201 aatgagcgaa gccgtgaaga ctgcattact gagtctggac ctgatccccg atgtcgatgt
     1261 ctccccgcaa atattccttg aggatgccgg taaggaagcc gtaacagtac ccaatcctgt
     1321 ttatgatttc actggaccgg taccctcagg gatcagagat tcactccatt caggtactat
     1381 tatgactgat cgtcagctgc ggcagacaga actcccgacc ggtatggttc cgatggatgc
     1441 gaaactaccc gtgatggagg ttatcgggca attggggggg atatatatcc tggccacaac
     1501 tgaatccggc gaacttgtgc ttatagacca gcatgccatt cacgagcgta tcctttacga
     1561 acaggttatt gcacggaccg caggcgaaca tcgttcgcag gaactgatag tcccgtttat
     1621 cctacacagg accctgcagg aaacggcaat actccgcgat cttttaccag cccttgctca
     1681 ggaaggattt gttcttgagg aattcggcag ggatactttt cttgtccgtg caattcctgt
     1741 ggttctgggg aagctcgagg acacatcaat aattgatgag atggtgagtg atcttgtaag
     1801 tgaagagccg gtccgttcag ttaacacccg tgaaagaatt acccgaataa tcgcatgcag
     1861 gggtgcaatc aaggcaggta ctgtatgcac acaggagcaa agccagcgat tgatacaaca
     1921 aatacggtat gcaaaaaatc cgttcacctg ccctcatgga agaccaacga tgctcaggtt
     1981 cagccggacc gagcttgata ttatgtttaa acgttcctga ttgatcatac tatcccgaga
     2041 tcctctcaca cctgaccccg gtatcgttgt tgccattacc tgaatcatta tgtgtagtca
     2101 cgtttatacg gtagggatcg agtcagaaat tatatctatg catgaaacat gaaaaaatct
     2161 catcaaaaaa atctggatca taccgagcct cagttttccc gtgatgatag ctccagctct
     2221 ggcattctgg cgttccagca gatggtgctg tcacattacg agcagtacgg tcgggacatg
     2281 gc
//
LOCUS       DASRLV010000078         5552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_264518,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000078 DASRLV010000000
VERSION     DASRLV010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5552
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264518"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="VFC43_03190"
     CDS             <1..776
                     /locus_tag="VFC43_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991283.1"
                     /note="functions to produce tRNACys in organisms that lack
                     cysteinyl tRNA-synthetases; SepRS; uses modified amino
                     acid; subclass IIc aaRS; attaches O-phosphoserine to
                     tRNACys; the product of Mj1678 converts the Sep-tRNACys to
                     Cys-tRNACys; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-phosphoserine--tRNA ligase"
                     /protein_id="HZK30224.1"
                     /translation="EKRSKYYMPDSQTEVYARHPVHGWVEVATFGMYSPSALAEYGIG
                     VPVMNLGLGVERLAMIAYNANDIRQLCFPQFFPHPLSDHEIACSVRLREEPITSDGKM
                     LAVAIARVASSNGSAQGPCSFDAWEGLLGGIKVKVTVEEPESNARLCGPACANEIFVH
                     EGSILGVPDAEKWKQVRTEGISTGITYLSAVSALAAARIEEAARCRKSTLVQVKMAKL
                     PSDINLKIDEFAMRAVTDNNKKVDVRGPVFLSVRAAVQE"
     gene            882..1268
                     /locus_tag="VFC43_03195"
     CDS             882..1268
                     /locus_tag="VFC43_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30225.1"
                     /translation="MKQRNLYRIIVGILMLISGLVSIVLASGEYVIGSILLVGGIAFL
                     SVGITRHFRDGESPESDERSKKIGAFGLSYGWLTGLFFMFALFWLDYLNFLRLGTQVA
                     LALSIVVLALSARVYQIYLFRKGDIE"
     gene            1291..1524
                     /locus_tag="VFC43_03200"
     CDS             1291..1524
                     /locus_tag="VFC43_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZK30226.1"
                     /translation="MQTRIKEYRSRQSMTQEDLAKAVGVRRETIVFLEQGKYNPSLKL
                     AHDVAMILHAKIDDLFIFGDETPEPELHEILVD"
     gene            complement(1521..3845)
                     /locus_tag="VFC43_03205"
     CDS             complement(1521..3845)
                     /locus_tag="VFC43_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZK30227.1"
                     /translation="MAVDDVIRLLRVNPVYRNRVVHTEITDPVSPKFGRLEIPLEDTL
                     SAYLSQHRIRLYTHQCDAINRIRLGKNVIITTPTASGKTLVFNIPVFERLEKDPDTRA
                     LYLYPTKALSNDQLETLEQMTQFTGIPARPAIYDGDTPQSKRPAIRENSRIIISNPHE
                     LHQVLSWHAKWRPFFTNLKFIVIDEAHRYRGVFGSHVALLIRRLIRLCHFYGSEPQFI
                     LSTATLANPLEFAKKLTGMPFELVDEDGSPRGRKHFVLYNPFFDGIGERSTYQETKDL
                     LVSCVKDQLQTLCFTGSRKMAELVTLWAREDARRSSPRLAESISAYRAGYLPEERRVI
                     EHQLKEGSLKGVVSTNALELGIDIGSLDAVIIAGYPGTMMSTRQQAGRAGRKGGESLA
                     VLVAMANPLDQYFMHHPHHFFSRSHEHAIIDTGNPYIVSGHLLCAAAELPLKDDSDRI
                     YFGESYTELLSELETGDLIRKTSRGWVYSGRGRAADAVRLDGIPGSTFRVMCHGRLLE
                     TLDRSQAYREAHKGAIMLHQGETYIVNEMDLETHTARVTETDVDYYTQPLKEVDLSII
                     ETLETRQVQGAQCAFGEVEVTERYTGYKIKRKDTIIGMEPLDLPPITFRTKAFWVIPA
                     ADTGQRIAHSDLDLAGGLHGAEHAIIALMPLHVMCDRWDIGGLSSPSFGENSEPTVFV
                     YDGYEGGIGLAEKAYEILPDVFSSAHELVRDCGCDEGCPSCIYSPKCGNDNQPLDKVA
                     TRLILRDLSRNPENAGLVFSHEVSEITSPETGPG"
     gene            complement(3835..4917)
                     /locus_tag="VFC43_03210"
     CDS             complement(3835..4917)
                     /locus_tag="VFC43_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HZK30228.1"
                     /translation="MSASHVSARIGALWQQRMETLKEYEVVRDGNVFRTSFSNTIVFS
                     SEYEKAYHQLDALVDKYRNVPFDIVIPGKEIFNECGVCFCQKSHKSLKIPSIDIERFR
                     SDILKDLTLVHGIGPRIQTRLKSKGFRTLPDLMQHPKFRSHTHEVLTCLNNGSSTDIM
                     NLIGCRHKKSHPSVLGTAGLHAPEDYVFLDIETLGLFSRPIILFGIGMIENGNLTVHQ
                     YLLRDIAEEQAALMATISHLSADRPALVTFNGKSFDIPYVSDRLAYYGMESLANIPHF
                     DVLHFSRRRWKDQFPSLRLTALEREVLGICRDDDIPGQMVPEFYEAYLKTGNCGPLVP
                     IIEHNRQDVVSLALLFFHLLEETYGC"
     gene            5137..>5552
                     /locus_tag="VFC43_03215"
     CDS             5137..>5552
                     /locus_tag="VFC43_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HZK30229.1"
                     /translation="MKKIRGFLIDLDGVLYVGNREIQGAREAIEFLSKRKFMFRFVSN
                     TTRKCRKTIADRLSSMGFDIPVEYIFTPPLAAVAFMKNSQKNRCFLLTTGDVYHDFEN
                     ECICDPVTPVDYVILGDAGDNVTYGSMNTAFRHLMNG"
BASE COUNT         1430 a         1315 c         1399 g         1408 t
ORIGIN      
        1 atgagaaacg ctccaagtat tacatgccag attctcagac agaggtttat gcacggcacc
       61 ctgtacacgg ctgggttgaa gttgccacat ttggaatgta ttcaccttct gcacttgctg
      121 aatacgggat cggcgtccca gtcatgaacc tcgggctggg tgtggaacgg cttgcgatga
      181 ttgcatataa tgcaaatgac atccggcaac tctgtttccc ccagttcttc ccccaccccc
      241 tttcggacca tgaaattgcc tgttcagtca ggctgcgtga ggagccgatc acatcggacg
      301 gaaagatgct cgcggttgcg attgcccggg ttgcctcatc aaatggatca gcccagggtc
      361 cgtgctcgtt cgacgcatgg gagggcctac ttggaggtat caaggtgaag gtgaccgtcg
      421 aagagccgga gtccaatgcc agactctgcg gtcctgcctg tgcaaacgag atcttcgttc
      481 acgaaggctc aattctcggt gtaccggatg cggaaaaatg gaagcaggtt cgtaccgaag
      541 ggatctctac ggggataacg tatctgtcgg ctgtatccgc acttgcggca gcacggattg
      601 aagaggcagc ccggtgcagg aaatctacgc tcgtccaggt aaaaatggca aaacttccca
      661 gcgatattaa cttaaaaatt gatgaattcg caatgagggc tgttactgat aataataaga
      721 aggttgatgt gcggggaccg gtgtttttat cggtgagagc cgccgttcag gaataatcat
      781 ttcacttttt tgggattatt gtattcagag gggatatgtt agaaatagat aacattaaaa
      841 accagcaatg ttagaaatat ataacaaaga atgtgaatgg aatgaaacaa aggaacctct
      901 atcgtatcat tgtcggaatt ctgatgctga tcagcggtct cgtatccatt gttctggcat
      961 caggtgaata tgttatcggg tctattcttc tggttggagg aattgctttt ctctcagtgg
     1021 gcatcacccg acatttcagg gatggcgaaa gcccggaatc tgacgaacgt tcgaaaaaga
     1081 ttggggcttt cgggttgtca tatggctggc tgaccggact cttcttcatg tttgctctat
     1141 tctggctgga ttaccttaat ttcttaaggc tcggaacaca ggttgcactc gcactttcga
     1201 tcgtagtgct tgccctttca gccagagtgt accagattta cctgttccgg aaaggagata
     1261 tcgaataaca gattttggag tgcatcaaca gtgcagacaa gaattaaaga gtaccgttcc
     1321 cgccagtcta tgactcagga agacctcgca aaagctgtgg gcgtacggcg ggaaacaatc
     1381 gtattccttg aacagggaaa gtataacccc tcgttaaaac tcgcccacga tgtggcaatg
     1441 atccttcatg cgaaaattga cgacctcttt atctttgggg acgagacacc ggaacctgag
     1501 ctgcatgaaa tactcgtgga ttaaccgggt cctgtttctg gtgaggtaat ttctgagacc
     1561 tcatggctga aaacaaggcc agcattttcc gggttgcgac tgagatctct cagaataagc
     1621 ctagtcgcta ctttatcaag cggctggtta tcattcccgc atttgggcga ataaatgcag
     1681 gacggacatc cttcatcaca cccgcagtca cgtaccagtt cgtgcgcact tgaaaaaacg
     1741 tcaggaagaa tctcgtaagc tttctctgca agtccaatcc ctccttcgta cccgtcataa
     1801 acaaaaaccg taggttcgct attctctcca aacgacggag acgataatcc gccaatatcc
     1861 cagcggtcac acatgacatg caggggcatc agagcgataa tggcatgttc ggccccatgc
     1921 aaccccccgg cgagatcgag atctgaatgt gctatgcgtt gcccggtatc tgctgcaggt
     1981 ataacccaga acgcctttgt cctgaacgta atcggtggca gatcaagcgg ctccatacca
     2041 attatggtgt ctttacgttt gatcttgtaa ccggtatacc gttcggtcac ttccacttcg
     2101 ccaaacgcac actgtgcacc ctgaacctga cgggtttcga gggtctcaat aatggagaga
     2161 tcaacttctt tgagtggttg cgtatagtaa tctacatcag tctcggtaac ccgtgcggtg
     2221 tgtgtttcaa ggtccatttc attgacaata tacgtctcac cctggtgcag cataattgcc
     2281 cctttatgcg cttcacgata cgcctgtgac cggtcgagtg tttcgagcag ccggccatgg
     2341 cacatgaccc ggaatgtcga accgggaatc ccatcaagcc ttaccgcatc tgcagccctg
     2401 ccgcgcccgg aataaaccca cccgcggctt gttttcctga taagatcgcc ggtctcaagt
     2461 tcagaaagga gctcagtata agattccccg aaatatatcc ggtcagagtc atctttcaaa
     2521 ggcagctccg cagcagcgca gagcaggtgc ccggaaacga tatacggatt tcctgtatcg
     2581 ataatcgcgt gctcgtgtga gcggctgaaa aaatggtggg gatggtgcat gaaatactgg
     2641 tcgagaggat ttgccattgc aacgagaacc gcaagtgact cccccccttt tctaccggct
     2701 cggccggcct gctgcctggt tgacatcatg gtcccggggt aacctgcaat gatgacagca
     2761 tccaatgacc caatgtcgat cccgagttcc agcgcattgg ttgatactac acctttcagt
     2821 gagccttctt tcaactggtg ctcaataacc ctccgctctt ccggaaggta accggcacgg
     2881 taagcggata tcgattctgc cagacgagga gaagagcggc gggcatcctc ccgtgcccag
     2941 agagtgacca gttctgccat tttccgggag ccggtaaaac agagcgtctg gagttggtct
     3001 ttcacgcagg agacgagaag atcctttgtt tcctggtatg tcgagcgttc gcctattcca
     3061 tcgaaaaacg ggttatacag gacaaaatgt ttcctgccac gcggagatcc atcctcatcc
     3121 accagttcaa acggcatccc cgtgagtttc tttgcaaatt ccagcggatt ggccagggtt
     3181 gcagtggaaa gaatgaactg cggctctgaa ccataaaaat gacacaggcg aatgagcctc
     3241 ctaatcaaca gagcgacatg ggatccaaaa acgccccggt agcggtgtgc ctcgtctata
     3301 acaataaatt tcagattagt aaagaaggga cgccactttg catgccatga aagtacctgg
     3361 tgcagttcat gggggtttga gataataatg cgggaattct cccgaatcgc tggccgtttt
     3421 gactgagggg tgtcaccatc gtatatcgct ggcctggcgg ggataccggt gaactgtgtc
     3481 atctgttcaa gggtttccag ctgatcgttg gaaagtgctt tggtcgggta caggtacaat
     3541 gcacgtgtat cggggtcctt ttcaagacgc tcgaacacgg gaatgttgaa tacaagtgtt
     3601 ttgccacttg cagtgggggt ggttataatc acgttttttc caagccgtat tcggttaata
     3661 gcatcacact gatgcgtgta aagacggatc cggtgctggg aaagatatgc tgaaagagta
     3721 tcctcaagag gtatctcaag tcttccgaat ttcggtgaca cagggtcagt tatttctgtg
     3781 tgtactacac ggttccgata aaccgggttc accctgagaa gcctgatgac gtcgtcaaca
     3841 gccataggtt tcctcgagca ggtggaaaaa aagtaaagcg agcgatacca cgtcctgcct
     3901 gttgtgttca ataatgggga caagcggacc acaatttccc gttttcagat aggcttcgta
     3961 gaattccggt accatctgcc cgggaatatc atcatcgcgg caaatgccca aaacttccct
     4021 ttccagtgcc gttaatctca gggatgggaa ttgatctttc caccttcttc tgctgaaatg
     4081 aagtacatca aaatggggaa tgtttgccag ggattccata ccgtaatagg caagcctgtc
     4141 tgacacatag gggatatcaa aggatttccc attgaatgtt accagtgcgg gtcgatcagc
     4201 tgacagatgg ctgatcgtcg ccataagtgc ggcctgttcc tcggcaatat cacgcaggag
     4261 atactggtga acggtaagat tcccgttttc gatcatcccg ataccaaaga ggatgatcgg
     4321 gcgggaaaaa agcccgagtg tttcgatatc caggaataca taatcttccg gtgcatggag
     4381 tccggccgtt ccgagaaccg aaggatggga ttttttgtgt ctgcacccga ttaaattcat
     4441 aatatctgtt gaactgccgt tattgaggca tgtgaggact tcatgggtat gtgaccggaa
     4501 cttcgggtgc tgcatcagat ccggcaatgt ccggaaccct tttgacttca gccgggtctg
     4561 gatccttggc ccgataccat gcacgagtgt aaggtccttg aggatatctg acctgaaccg
     4621 ttcaatatct attgaaggaa ttttaagcga tttgtggctt ttctggcaga aacagacacc
     4681 gcattcattg aaaatttctt tgcccggaat gactatatcg aacggaacgt tccggtactt
     4741 gtcaaccaat gcgtcaagct ggtgatacgc tttttcgtat tccgaagaaa aaacaatagt
     4801 attcgaaaaa cttgttctga aaacattccc gtcacggaca acttcatatt ccttgagggt
     4861 ttccatgcgt tgctgccaca gggcaccaat ccgtgcagaa acgtgcgaag ccgacatcgt
     4921 ctgaatacaa aaatgctgtc cggggcttaa atatccagcc gcggggagtg aaagtgatat
     4981 tcaccgggat tcccaatact tgcccggatt tttttaaagt acaaacccag ggtcagcttt
     5041 tatgacaaaa ggatattccc gcaacaaaaa agaatttgtg agtacccggg acaaaaaaaa
     5101 tttccggaac ggtaatactg caggagagga caaaaaatga aaaaaatcag aggatttctc
     5161 atcgaccttg atggggttct gtatgtagga aacagggaga tccagggtgc gcgggaagct
     5221 attgaattcc tcagcaaaag aaaatttatg ttcaggtttg tttccaacac cactcgtaaa
     5281 tgccggaaaa cgattgctga tcggttatca tccatgggtt ttgatattcc cgttgagtac
     5341 atattcactc cacctctggc tgcagtagcc ttcatgaaaa acagtcagaa aaatcgttgc
     5401 ttcctcctca caacgggtga tgtgtaccac gattttgaga atgaatgcat atgtgatcct
     5461 gtaactcctg tggattatgt aatcctggga gatgccgggg acaatgttac ctatggcagc
     5521 atgaacaccg cgttccgtca tctgatgaat gg
//
LOCUS       DASRLV010000079         2396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_834214,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000079 DASRLV010000000
VERSION     DASRLV010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834214"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1639)
                     /locus_tag="VFC43_03220"
     CDS             complement(<1..1639)
                     /locus_tag="VFC43_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZK30230.1"
                     /translation="MMRNFNRWLIIAIIAILASGMVLTLWTVQREENLMRSDLLAKTR
                     LLQGGFTPGYVNALTATENDLASPDYQVLKRQLIQLKSSNPEIRFLYLVGQKPDGTVF
                     FFIDSEPPESNDYSPPGQLYPEASPVLLNTFASEEATTGGPVTDRWGTWVSGLVPIRD
                     TNNGKVIAVLGTDIDARDWNLQIIKASAPAVVVMLLLLLLMLILFYMQQRNARERQIL
                     TTSEAAIRESENRYRKVFESTGTAMVIVEDDTTISLANNEFFRLTGYSPEDITRKMSW
                     TEFVFEDDLKKMKDEHRLRREKPEEALKQYEFRLITKQGELRNILLTIDILPDTLKSV
                     GSLMDISEIKEIHAVLERHASELKRNADVLRQTNNKLNILNSITRHDILNQLTAILGY
                     LELTKMKYPDPSLQEFINKEIQAAETIKAQIMFTKDYQNIGVRSPQWFDLKKVILASA
                     ALLPLSTVKLDVDCDTLEIYADPMLEKVFYTLLENALRHAKTITTIEFSCCKLAEGLR
                     VIYQDNGEGIPAEYKEAIFEHEYFKHTGFGLFLSRAIL"
     gene            complement(1746..2117)
                     /locus_tag="VFC43_03225"
     CDS             complement(1746..2117)
                     /locus_tag="VFC43_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZK30231.1"
                     /translation="MEDADKKILIVENDEIVSKVLEWRLTGLGYAVSGKASTGQDAIT
                     LTNETRPDAILMDIGLYGSMDGIEAAKIIQEKFNIPIIFLTAHSDATVLERIFPLKPA
                     NFITKPFTDDDLRVALRLSID"
     gene            complement(2117..>2396)
                     /locus_tag="VFC43_03230"
     CDS             complement(2117..>2396)
                     /locus_tag="VFC43_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK30232.1"
                     /translation="ITTIRVASEVHDGNWIIIIEDDGVGIPLEEKEKIFEYGFGKNTG
                     MGLFLTREILGITGITIRETGEPGKGARFEVMLPKGIWREEKMKTGGS"
BASE COUNT          575 a          585 c          550 g          686 t
ORIGIN      
        1 ccagaatagc acgcgaaagg aataacccaa agccggtgtg cttgaaatac tcgtgttcga
       61 aaattgcttc cttatactca gcggggatac cctctccatt gtcctggtat attaccctga
      121 gtccctctgc cagtttacaa caggaaaatt cgatggtggt aatcgtttta gcatggcgga
      181 gagcgttttc aagcagagtg tagaagacct tctcaagcat cggatctgca taaatctcaa
      241 gagtatcaca gtccacgtcc agtttcactg tggaaagcgg aagaagggct gcactggcca
      301 gaattacctt tttgagatca aaccattgcg gggaccggac cccgatattc tggtaatctt
      361 tggtgaacat gatctgggcc ttaattgtct cggcggcctg aatttcttta ttgatgaatt
      421 cctggaggga gggatccgga tatttcattt tcgtcaattc cagatacccg agaatagccg
      481 ttagctggtt gaggatgtcg tgccgtgtta tactgtttaa gatgttcagc ttgttgttgg
      541 tttgccggag aacatccgca ttacgtttca gctccgaagc atgccgttcc agtacagcgt
      601 gaatctcctt tatttcagaa atatccatca gtgatccgac gctttttaag gtatcaggca
      661 atatgtcgat ggtcagaagg atgttgcgta gttccccttg ttttgtaata agccggaact
      721 cgtattgttt caaagcctct tccggctttt cccggcgtaa ccgatgctca tccttcattt
      781 ttttgagatc gtcttcgaac acgaattctg tccaggacat cttacgggtg atatcctcag
      841 gggaatagcc tgtcagccgg aaaaactcat tattggcaag gctgatggtg gtatcgtctt
      901 caacgatgac cattgccgtg ccggtgcttt caaagacctt ccggtaccgg ttttcactct
      961 cccgtatggc agcttcagac gttgtaagga tctgccgctc ccgggcattt cgctgctgca
     1021 tatagaataa tatcagcatg agcagcagga gcaggagcat tacaacaacg gcaggtgcac
     1081 tggccttgat aatctgtagg ttccagtccc gggcatcaat atctgttccc aatacagcta
     1141 tcaccttacc attattggta tcccgtatcg gtacgagccc gcttacccac gttccccagc
     1201 gatcggtcac cggtcctccc gtggtagctt cttctgaagc aaatgtattc agcaggacag
     1261 gagacgcctc aggatacaat tggccgggcg gcgagtaatc gtttgattcc ggaggttccg
     1321 aatcaataaa gaaaaaaacc gtcccgtccg gcttctgtcc cacgaggtag agaaatcgga
     1381 tctcaggatt ggaggatttg agctgtatca gttgtctttt taacacttgg taatccggag
     1441 atgccagatc attttcagta gctgttaacg cgtttacgta cccgggagtg aacccgccct
     1501 gcaaaagacg ggtttttgca aggagatcgg aacgcatgag gttttcttcc cgctgaaccg
     1561 tccacagggt cagaaccata ccactggcaa gtatggcgat gatagcaatg atcagccatc
     1621 tgttgaaatt gcgcatcatt cttcaacctt ctcctggatc cctgtatcgc atctctttag
     1681 catcgtgtcc ggttttttgt cttgtgtttt tgaaaatgac ctgaatgaat gtaatcttat
     1741 catgatcaat cgatcgataa tctgagtgcg acccgcaggt catcatctgt aaatggtttg
     1801 gtgataaagt ttgcaggttt gagagggaat atcctttcaa ggacggtagc atcagaatgc
     1861 gcagtaagga atatgattgg aatattgaat ttttcctgaa tgattttagc ggcttcaatg
     1921 ccatccatcg acccgtacag accaatatcc atgagaatgg catcaggtct ggtttcattt
     1981 gtcagggtaa ttgcatcctg acctgtagat gctttaccac tgactgcata ccccagcccg
     2041 gtgagtcgcc attcgagcac tttactgacg atttcgtcat tctcaacaat caggattttt
     2101 ttgtctgcat cctccattaa ctccctccag ttttcatctt ttcctcgcgc catatcccct
     2161 tcggtaacat aacttcaaac cgggcacctt tgcccggctc cccggtctcg cggatggtga
     2221 taccggttat gccaagtatc tcacgggtaa gaaagaggcc cataccggta tttttcccaa
     2281 agccatattc aaagatcttc tccttctctt caagaggtat acctactcca tcatcctcaa
     2341 tgatgatgat ccagttcccg tcatgcactt cagaagcgac ccggattgtc gttatc
//
LOCUS       DASRLV010000080         6929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1079555,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000080 DASRLV010000000
VERSION     DASRLV010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 6929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6929
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079555"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1149)
                     /gene="fen"
                     /locus_tag="VFC43_03235"
     CDS             complement(148..1149)
                     /gene="fen"
                     /locus_tag="VFC43_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease-1"
                     /protein_id="HZK30233.1"
                     /translation="MGVALRDIIADYKTPVTWESLPGIAAIDANNALYQFLTIIRQPD
                     GTPLMDQMGRVTSHLSGILFRVSNFMEKGIKPIFVFDGKPTALKQDTINGRRKIRDEA
                     GEKWKEAIERGDEAEAYKQARSSSRVDSKIIETSKELLRLMGLPTIQAPGEGEAQAAC
                     MVGKGEARYVVSQDYDTLLFGAPSLVRNLTVSGKRKIRGRQIVVNPERLILSDVLTGL
                     KLTREQLIEIGILIGTDFNPGVEGIGAKTGLKIVQRGEFDAKIKEKLPDFDPAPVMDL
                     FLHPDVTDDYTLVWGRPDIAGIREMLCGDYDFSKERVEKAMENFCAKAGQKTLESWF"
     gene            complement(1172..1486)
                     /locus_tag="VFC43_03240"
     CDS             complement(1172..1486)
                     /locus_tag="VFC43_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30234.1"
                     /translation="MDIREYLIDILLTIVMIISTLVLINRFWQDLTIAIAATLMMLSL
                     GGLFLSLQIKMRHLERSIVLRERILRNNLEEISRRMAEKIDMSMTHFDELVGEYSKRM
                     YR"
     gene            1544..2437
                     /locus_tag="VFC43_03245"
     CDS             1544..2437
                     /locus_tag="VFC43_03245"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042500 - aspartic endopeptidase activity,
                     intramembrane cleaving [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presenilin family intramembrane aspartyl
                     protease PSH"
                     /protein_id="HZK30235.1"
                     /translation="MDLKQIIPLLVMPLLLLAVEIGSILISLPVQAAGITAFEDPTSI
                     ANPFIFIAILLVFTGFLLVLIKFDLKKVIAAIIGFSIFLTFGYIFAALVTAALGTTDF
                     AMVVVLVLSILATALLYIFPEWYVIDSLGILIGAGVASIFGVSLDILPVIILLALLAV
                     YDAISVYKTKHMITLAEGVIDLKTPILFVVPKRRDYSFIRDGIGKLTEGGERGAFIIG
                     MGDLIMPSILVVSANVFLQGWRLLGIINLPALGAIIGSLVGLGVLLSYVASGKPQAGL
                     PPLNGGTILGFLVGWVLMGLI"
     gene            complement(2552..3622)
                     /locus_tag="VFC43_03250"
     CDS             complement(2552..3622)
                     /locus_tag="VFC43_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HZK30236.1"
                     /translation="MEFEKIPTVPTADEILDRSFRRAAKKMKEKTNKDRANEEFVRAV
                     GAAVHDRLVYIIRGFPEFDQIPLFYREMADILFGMDRIRQSLGAVGWAAKHTKMVGNQ
                     LAVQTRKSDDTTLVRKRTVARLASMVHQINKDLLFLNEVRNVLRKLPHVEDAYTIVIA
                     GYPNVGKSSFIRQVSSAAPEIASYPFTTKGIIVGHRNIGRDRIQFVDTPGILDRPAEE
                     RNTIERQALSAMMNVANIILFILDPSEHCGYPMEVQLNLLDEVKGMIKVPLVVVANKS
                     DLQVVDGYLSMSTENGEGIETVLAAILEHKPETVEQKRSKVPDIRSPLVYEPEETEMD
                     EKGEKVSKKRLHRKPRTPRAEI"
     gene            3670..4548
                     /locus_tag="VFC43_03255"
     CDS             3670..4548
                     /locus_tag="VFC43_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZK30237.1"
                     /translation="MDLSLIQRDILITLITLYHKHSRSVKGEDIADMIQRNPGTVRNQ
                     MQALKTIGLVDGVPGPKGGYIPTALAYKELNLNVSEGDYDVSISRDGEIISGATVREI
                     DFTTLCHPDICHAVIKLFGSAKLFEIGDQITIGPTPVNKLLLRGEVFGKDESEQLLLI
                     ATTEMISLPKQAIKAYMSSPLKTLTSSNTLRDALVLFNKNRIHGAPVIDKEQLKGIIT
                     MSDIAKAVEKDLPLTTKVSAVMTTDVIEAPSDIKLFEVIKRFKERKVGRLIVVEQGKP
                     VGILTQSDIIRVFPSL"
     gene            4600..4809
                     /locus_tag="VFC43_03260"
     CDS             4600..4809
                     /locus_tag="VFC43_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone"
                     /protein_id="HZK30238.1"
                     /translation="MTELSQSAVERIMKKAGAERVSADAAETLSRLMEDYGIFLAKEA
                     KKMSDHAGRKTLRGSDIRMAAEIFK"
     gene            4898..5152
                     /locus_tag="VFC43_03265"
     CDS             4898..5152
                     /locus_tag="VFC43_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HZK30239.1"
                     /translation="MTRVFARSLSRKKIMSNDGKLVGTLKNIRVDFDSGQVQAMIVHP
                     DQTFVTDGYNMEDDKLLVIPFESVKDIKDYIVVDRFLAKK"
     gene            complement(5116..5832)
                     /locus_tag="VFC43_03270"
     CDS             complement(5116..5832)
                     /locus_tag="VFC43_03270"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HZK30240.1"
                     /translation="MLKALLTDIDGTITDSSRRIHTGAVEAIRSLVDEGIDVVLASGN
                     TSCFMDALCKMIGTQGTFIAENGGVFRVGFTGTLHIKGDQGVCKKALETLQGYYHKKG
                     TDLVLFSPSYRFSDLAFARTVPVEEVKNILMDQPVTVLDTGYAIHLQSPGIDKGTALD
                     ALAKEMGLVPSDFLAIGDSLNDIQMLKKARIGVTVANGHPDIKAVAEFIAQKEYGPGF
                     VEAIAKYSSYFLARNRSTTI"
     gene            complement(5826..6800)
                     /gene="radA"
                     /locus_tag="VFC43_03275"
     CDS             complement(5826..6800)
                     /gene="radA"
                     /locus_tag="VFC43_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadA"
                     /protein_id="HZK30241.1"
                     /translation="MAEGPLEIEDLPGVGPSTAEKLREAGYLSVESIATASPSELSEI
                     SEISESVAKKIVKAAREAADIGGFKTGKDIFEQRKDVRKLSFRVPELDALMGGGLETQ
                     AITEMYGEFGSGKSQIVHQMAVNVQLNDEQGGLNGSAIYIDTENTFRPERIEQMVNGL
                     GIDDADPQEFLNNIHIARAHTSDHQMLLVENSRDLAAELKDSGRPVKLFIIDSLTAHF
                     RAEYAGRGTLATRQQKLNRHMHELFKLIDEHNAVALVTNQVMSNPAVFFGDPTKPIGG
                     NIVGHTATFRLYLRKSKGGKRIARLVDSPNLPEGEAPFMVEEAGLKSC"
BASE COUNT         1654 a         1771 c         1656 g         1848 t
ORIGIN      
        1 acatgatgag tgcatacatg agaaaattca taatcatctc tcaaagttct cctgaattta
       61 tcatgatgga tgtgatcagc agggatggtt accgcaattc ctggcagaaa aaataccccg
      121 tgctcaccct ttcacggcta aaatatatta gaaccagctc tcgagggtct tctgcccggc
      181 ctttgcgcag aaattctcca tggctttttc cacccgctct ttggaaaagt cataatctcc
      241 gcagagcatc tcccttatgc ccgcaatatc gggacggccc catacaaggg tataatcgtc
      301 cgtgacatcc gggtgtaaga agaggtccat aaccggtgcc gggtcaaagt cgggcagttt
      361 ctctttgatt tttgcgtcaa actccccccg ctgcacgatc ttcagcccgg tctttgcccc
      421 gattccctct acacccgggt tgaaatcggt gccgataagg atcccgatct cgatcagctg
      481 ttcgcgggtc agtttcagcc cggtaagcac gtcggagagg ataagccgtt ccgggtttac
      541 cacgatttgc cgtccccgga tcttccgctt gccgctgacg gtcaggttac ggacaagact
      601 gggggcgcca aagaggaggg tatcatagtc ctgcgatacc acgtaccgtg cctccccttt
      661 tcccaccatg caggctgcct gggcttcccc ttcacctggt gcctggatgg tcgggaggcc
      721 catgagacgg agaagttctt tggaagtctc tatgatcttg gaatcaaccc tggatgatga
      781 ccgcgcctgt ttgtacgcct cggcctcatc tccccgctcg atcgcctctt tccatttctc
      841 ccccgcctca tcccgtatct tacggcgccc gttgatggta tcctgcttga gtgccgtggg
      901 tttcccgtcg aacacaaaga ttggctttat tcccttttcc ataaaattcg agacccggaa
      961 gaggatgccc gataggtgtg acgttacacg ccccatctgg tccatcaggg gagtcccgtc
     1021 gggttgcctt atgatcgtca ggaactggta gagggcattg ttcgcgtcga tcgctgcgat
     1081 accgggaagc gattcccacg taaccggtgt cttataatcc gcaataatat cccgcagtgc
     1141 aacgcccata atgattcaca aaataaaaaa tttacctgta catacgtttt gaatattccc
     1201 cgaccagttc atcaaagtgg gtcattgaca tgtcgatttt ttctgccatc ctccgggaga
     1261 tctcctcgag attgttgcgc aaaattcgtt cacggagcac aatactcctt tccaggtggc
     1321 gcatttttat ctggagcgag aggaaaagcc ctcccagtga aagcatcatg agcgttgctg
     1381 ctattgctat ggtcagatcc tgccagaacc ggttgataag gacgagtgtg gaaattatca
     1441 ttacgatagt gagcaggata tcgattaagt actcacgaat gtccatactt ttagatgatg
     1501 agagcacagt aattaatcta actacttaag cggagaatgc agcatggacc ttaaacagat
     1561 aatccccctc ctcgtaatgc cactcctgct ccttgcagtg gaaatcgggt caatcctgat
     1621 ctcgcttcct gtgcaggcgg caggaatcac tgcatttgaa gatcccacat cgattgcaaa
     1681 tccttttatt tttatcgcga tcctgctggt attcaccggc tttttactgg tcctgatcaa
     1741 attcgatttg aaaaaagtga ttgccgcgat tatcgggttc tcgatctttc tcacgtttgg
     1801 ctacatcttt gcagctctcg tgacagcagc tctgggcaca actgactttg cgatggtcgt
     1861 tgtgctggta ctatcgattc tggcaacagc cctcctgtat atatttcctg aatggtacgt
     1921 gatagacagc cttggaatcc tgatcggtgc cggggtggcg tcgatattcg gagtctcgtt
     1981 agacatcctc ccggtgataa tccttctcgc acttcttgcg gtgtatgatg cgatctccgt
     2041 gtataaaaca aaacacatga ttacgcttgc tgaaggggta atcgacttga aaacccccat
     2101 cctgtttgtt gtgccaaaac ggcgtgacta ctctttcatc cgcgacggca tcgggaaact
     2161 gactgaaggc ggcgagcggg gagcctttat tatcggtatg ggagacctga tcatgccctc
     2221 aatcctggtg gtctccgcaa acgtcttttt acaaggctgg cggcttttag ggattatcaa
     2281 cctgccggcg ctgggggcca taatcgggtc ccttgttggt ctgggtgtct tgctctccta
     2341 tgtagcttca ggtaaacccc aggcagggtt gccccccttg aacggcggta ctatcctggg
     2401 gttcctcgtg ggctgggtgc tgatgggcct gatataaaaa accggatact tacaataatc
     2461 ccatggacgt tgttctgata aacgccacta cgttttttat gttcttacga aataaaaaaa
     2521 agatgatccg ggtcccgtta aaacagggat ttcatatctc cgcacgagga gtacggggct
     2581 tccggtgcaa ccgttttttt gagacttttt cgcctttttc atccatttcc gtttcttctg
     2641 gttcatagac gagaggggac cggatatccg gaaccttact ccttttttgt tccaccgttt
     2701 cgggcttgtg ctccagtatc gcggcgagta cagtctcaat tccctccccg ttttctgttg
     2761 acatggacag gtagccgtcc acgacctgca ggtcggattt gtttgccacg accaccaggg
     2821 gcacttttat catccccttc acttcgtcaa gcaggttgag ttggacttcc atcgggtacc
     2881 cgcagtgctc tgaagggtca aggataaaga ggatgatatt tgcgacattc atcatggcag
     2941 acagtgcctg ccgttcaata gtatttcgtt cttctgcggg ccgatcaaga atgcccggtg
     3001 tgtcaacaaa ctggattcgg tccctaccga tattccggtg tcctacaata atgccttttg
     3061 tcgtaaacgg gtaggatgcg atttccggtg cagctgaaga gacctgccgg ataaaggacg
     3121 atttacccac attggggtat cctgcgataa ctatcgtgta tgcatcttcg acatggggga
     3181 gttttcgtaa gacattcctg acctcgttta agaagagcag gtccttgttt atctggtgca
     3241 ccatggaagc aagccgtgca acggtccttt tgcgcaccag ggtcgtatca tcagatttcc
     3301 gtgtctgaac agccagctgg ttgcccacca ttttggtatg ctttgctgcc cagccgacag
     3361 cgcccagtga ttgtcgtatc cggtccatgc caaagagaat atcggccatc tcccggtaaa
     3421 aaagggggat ctggtcaaat tcaggaaatc ccctgatgat gtagaccagg cggtcatgga
     3481 ctgccgcgcc aaccgcccgc acaaattctt cattggcccg gtctttattg gttttttcct
     3541 tcattttctt ggccgcacgg cggaaactcc tgtcaaggat ttcatctgcc gtcggtactg
     3601 taggtatttt ttcgaattcc acaaatctcc aaccttttta tagtatggga agttataccg
     3661 tttatcgtta tggacttgtc gctcatccaa agagatatcc ttatcacact cattactctt
     3721 taccacaagc attcccgttc tgtaaaaggt gaagatattg ctgacatgat ccagcgcaac
     3781 ccggggactg tgcgcaacca gatgcaggcg ctcaagacca tcggacttgt ggatggtgtg
     3841 cccggcccga aagggggtta catacccacc gcccttgcct ataaagaact taacctgaat
     3901 gtatcagagg gtgactatga tgtcagcatc agccgggatg gtgaaattat ttccggagca
     3961 accgtccggg aaatcgattt taccacgctc tgccacccgg atatctgcca tgcagtgatc
     4021 aaactgtttg ggagtgccaa gctctttgaa ataggggacc agattacgat cgggcccacg
     4081 cctgttaata aactcctcct ccgcggggaa gtattcggga aagatgagtc cgaacaactg
     4141 ctgctcattg ccacaaccga aatgatctcg cttcccaaac aggcgattaa agcctatatg
     4201 agtagtccgc tcaaaacgtt aacaagttcc aatacgctcc gcgatgccct tgtcctgttc
     4261 aataagaatc gaatccatgg tgccccggtt atcgataagg agcagcttaa agggatcata
     4321 accatgagtg atatcgcaaa agcggtggaa aaagacctcc cactgacgac aaaagtatct
     4381 gcagtcatga ccactgatgt cattgaagct ccctcggata tcaaactttt tgaagtaata
     4441 aagaggttca aggaacgtaa agtcgggaga ctgatcgtag tcgaacaggg taaacctgtg
     4501 gggatcctta cccagtctga tattatccgg gtattcccct ctctttaaac aatatattta
     4561 cataatctga accaaattac ttgcaaggac tgagtaaaaa tgaccgaatt atcccaatct
     4621 gccgttgaac ggatcatgaa aaaagccggt gccgagcggg taagtgccga tgcggctgaa
     4681 accctgagca gattgatgga ggattatggg atattcctgg caaaggaagc aaaaaaaatg
     4741 tcggatcacg caggaagaaa gaccctgcgg ggatctgata tacgcatggc ggctgaaatc
     4801 ttcaaataac tgatattggg tttttaatat caaaaaaacc attttttttg cgccatttta
     4861 gaacacttta aatatttata cgagtcatat tttttttatg actcgtgtat ttgcccgcag
     4921 cctttcccga aaaaagataa tgagcaatga tggcaagctc gttggaacac taaaaaatat
     4981 cagggttgat tttgattccg gacaggtcca ggccatgatt gtccacccgg accagacctt
     5041 tgttaccgac ggatacaaca tggaagacga taagcttctg gtgataccgt ttgaatcggt
     5101 aaaagacatc aaggactata ttgtcgtgga ccggttcctt gccaagaaat aagaagaata
     5161 tttggcaatt gcttcaacaa accccggtcc atactctttt tgtgcaataa attcggcaac
     5221 tgcctttata tcgggatgcc catttgctac cgttaccccg atcctggctt ttttaagcat
     5281 ctggatatcg ttgagtgagt ccccgatcgc gagaaaatct gagggcacaa ggcccatttc
     5341 ctttgcgaga gcatccagtg ctgttccttt gtcgatgccc ggtgactgca gatgaatcgc
     5401 ataaccggta tcaagcacgg taaccggctg gtccataagg atatttttaa cctcctcaac
     5461 aggaaccgtg cgggcaaaag ccagatcgga aaaacggtac gaggggctga acaggacaag
     5521 gtctgttccc tttttatgat aataaccctg cagcgtttcc agggcttttt tgcaaacgcc
     5581 ctgatcgcct ttgatatgga gagtgccggt aaaaccaaca cggaacactc ccccgttctc
     5641 tgcaataaac gtcccttgag tccctatcat cttgcagagg gcatccataa aacaggatgt
     5701 attcccgctg gcaaggacta cgtctatacc ctcatccaca agcgacctga tcgcttcgac
     5761 agcaccagta tgtatacggc gggacgaatc cgttatcgtc ccgtcaatat cggtaaggag
     5821 tgcctttagc acgatttgag acctgcttct tctaccatga atggagcttc accttcggga
     5881 agattgggac tgtcgacaag acgtgcaatg cgttttcccc ctttactctt gcgcaggtag
     5941 agccggaagg ttgccgtgtg tcccacaatg ttgcccccaa tgggttttgt cgggtcaccg
     6001 aaaaagactg ccgggttgga cataacctgg ttggtcacca gtgcaacggc attgtgctca
     6061 tcaatgagtt tgaagagctc atgcatgtgc cggttcagtt tctgctggcg tgtcgcgagt
     6121 gttccccgcc cggcatattc agccctgaag tgagcggtga gcgaatcaat gatgaagagt
     6181 ttgaccggcc gcccgctgtc tttcaattcc gctgcaagat cccgtgaatt ttccacaagg
     6241 agcatctggt gatccgaggt gtgtgcccgt gcaatgtgga tgttgttcaa aaactcctgc
     6301 ggatccgcat catcaatccc cagcccgttc accatctgtt caatacgctc gggacggaat
     6361 gtattctcag tgtcaatata aatagcactc ccgttcagtc caccctgctc atcattgagc
     6421 tgaacattga ctgccatctg gtggacgatc tggctcttac cggaaccgaa ttcaccatac
     6481 atctcggtga tggcctgtgt ctccagtcct ccccccatga gggcatcgag ttcgggaacc
     6541 cgaaacgaca gtttcctgac atcttttcgc tgttcgaaga tgtcttttcc ggtcttgaac
     6601 ccgccaatat ctgcggcttc acgtgcggct ttgacaatct tttttgccac cgactccgag
     6661 atctccgaga tctcggaaag ttccgacggt gatgcagttg caatactctc aacggagaga
     6721 tatcccgctt cacggagttt ctctgcagtg ctcgggccaa cgcccggtaa atcttcaatt
     6781 tctaaaggtc cttcagccat ttcttgcaca tctcctatgg cacatcaaga tgttaaacat
     6841 gggcttatat gctctcggac tgcgtggggt gaaagtgtaa ttcaggtatc cagttcttgt
     6901 atgaaactct gcagctgcgc cagtacagg
//
LOCUS       DASRLV010000081         7492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_190545,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000081 DASRLV010000000
VERSION     DASRLV010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 7492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7492
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190545"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..126
                     /locus_tag="VFC43_03280"
     CDS             7..126
                     /locus_tag="VFC43_03280"
                     /inference="COORDINATES: protein motif:HMM:NF015909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30242.1"
                     /translation="MLARTNTSYAPWVVIESDNKWYARIKAVKEVIRYAEKLL"
     gene            587..934
                     /locus_tag="VFC43_03285"
     CDS             587..934
                     /locus_tag="VFC43_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30243.1"
                     /translation="MSTDLLQLIGIVKLPSGMNQDAFEHQMKQAQQLKWIDVNGKTTN
                     DTISGRKVVHVYVKTLKNSDPNLVVPYTWNVSDSDPSNNIIVAWVEPDNLPKLASLDD
                     VQSIWTVLQPINN"
     gene            979..3366
                     /locus_tag="VFC43_03290"
     CDS             979..3366
                     /locus_tag="VFC43_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HZK30244.1"
                     /translation="MREHIIAICTIIVMAGLLIIPVTASLHESYGEGKNITFLISSGK
                     INSPDVSIDKNYLTESERKLSSTLLKQTLKQTSASSLATPSQINGSFESSLRIKNVPV
                     PSGSDGSPKESPNLISGKLVYAYVYVKPGYSTHIIDSSVFEVSGRDENYHSAVAWIDV
                     QALEPLSSLEGVRTIREVIPPMVNTGSVTTAGDTIHKTATVRSTYGNRGAGMKIGIIS
                     DGVDNLASAVASGDLPSDVTVLSNTEGGDEGTVMLEIVHDMVPDAELYFHDQGDSSIA
                     FNSAIDDLVANGCTVICDDVSLFAEPYFEDGNVASHIKTLLAGNNLVYISSAGNDGST
                     NTAATRHHYQGSYYESETTNWNDFSQGSSSTPSLYLNLSSGSTVTVILQWDDPWGHSG
                     NDYDLYLHAMNGSIITSSQLVQSGTSDPIEILSYTHSGPITDAQINVFNYNGLAQSRN
                     LELYIITGGSTLVYTNNINCTDSIFGQPAVTDVIAVAAVPASSPATIESFSSRGPVTI
                     SYPSPETRIKPDISGVDCVAVTGVGGFGSPFCGTSASAPHIAAVVAQYWGAHPGLTPA
                     QVRTALYTNAADLGTSGKDTVFGYGLADALNMESHFSGGSGGSIAVVEKSFHFGTSGD
                     IPVAGDWNGDGIQDTGVFRPSNGNWYLDTTKTGVVNRTFHFGTTGDTPVTGDWNGDNV
                     SDVGVFRNSNGNWYLDTTKTGVVNQTFRFGKNGDTPITGDWNSDGISDIGVFRNSNGN
                     WYLDTTKTGVVNRTFLFGKNGDMPVIGDWNSDGVSDIGVFRNSNGNWYLNYTPQI"
     gene            complement(3910..4491)
                     /locus_tag="VFC43_03295"
     CDS             complement(3910..4491)
                     /locus_tag="VFC43_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30245.1"
                     /translation="MVSLQRQGCHNINLVTPSHVVPQILKSLSIAADQGLCIPIVYNS
                     GGYDAIDTLMLLEDVVDIYMPDAKYGSEEIARLLSHAPDYVITMKAAIREMHRQVGDL
                     VIDEGIAVRGMIIRHLVLPENLAGSNKVLPWIAEEISCDSYVNIMEQYHPAWHTTGIE
                     KGSLPSSLWRRITPGEYHFAILIARDSGLHRVF"
     gene            complement(4894..6087)
                     /gene="thiI"
                     /locus_tag="VFC43_03300"
     CDS             complement(4894..6087)
                     /gene="thiI"
                     /locus_tag="VFC43_03300"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HZK30246.1"
                     /translation="MSGQEKLVMVRYGELFLKSESVKHHFIGMLLRNMGKALTASSLS
                     YHYETPRGRILIYGDEPERIAEVVSRIFGIVDVSICTRTASGLEDICTVAISLASANL
                     VRGMSFAVRAKRQQKTGLNSQELGTVIGSAVYDHIPGLRVDLNNPDYELFVEIRDFGG
                     LVYDSRIPAPGGLPWGTQGRVLSLLSSGIDSPIASWLMMKRGCEITNLHLDAGRWAGA
                     DVTRAAIENHRRLSRWCAGNPLSLVIANSELLYDCMDKLRIPPRYRCVICKRFMLRVA
                     GELMRNEGALAIVTGENLGQVASQTLANLGVISEAVKEPVLRPLITYDKEETVALARM
                     IGTFDAHPGDLACRAVPHMPATAAVLKAVQESEETLGIDAIIASTLCAIRYVTALNGE
                     IVKEI"
     gene            6247..6879
                     /locus_tag="VFC43_03305"
     CDS             6247..6879
                     /locus_tag="VFC43_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2150 family protein"
                     /protein_id="HZK30247.1"
                     /translation="MAKKASKKIEAEPLRLFYIFYNQERWDNWLKTLSESSFEADPKS
                     DELPEGFRILDGFSVDITVSVLKIIKLYQNKRFSFEEAVDKLSQIETIVMSAAPEGEL
                     GEIVEILQLPKLVLFASCRLYLAGGFNKDIKTLVKKGREDIQKDMEQALEAAANIGAS
                     VIDGASCCGKYVKDDIEETTLFDEWLIECERMGEAMVSLKNFDESTGDED"
     gene            6876..7328
                     /locus_tag="VFC43_03310"
     CDS             6876..7328
                     /locus_tag="VFC43_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5814 domain-containing protein"
                     /protein_id="HZK30248.1"
                     /translation="MIADRARFRHAKKLERLAGFRLPELAFSGTMLETLASRLDYDAL
                     DFSVREQILAFFDDFLRCDCKESPLCGCPEKKFAKKVIELRENGLDHRQIAGFMLDEY
                     GIEMFPTDVLSFLEESVHVLEAISDVARLQGSAELAKKTDEHIHLIER"
BASE COUNT         2063 a         1699 c         1711 g         2019 t
ORIGIN      
        1 gatgagatgc ttgcacggac aaacacctcc tatgcaccat gggttgtcat agagtctgac
       61 aataaatggt acgcccggat caaggccgtc aaagaagtca tccgctatgc ggagaaactt
      121 ttataaattt tattaatcac ccgtaaattt aatttttatc tgttttgcgg atttgcttaa
      181 tttcgatctt tttgaatctg ttgtatcaga tctcactttt ttttcgtata cctccatatg
      241 atcacaaatt cggtattaat tatagcagca tccgccagaa tctgatatcg tgttcccttc
      301 ttttccgatc agaaaaaaat caggaccaca caatttcgaa agggttatga atattaaaaa
      361 gaattcgaag ctaatgtctg atgagcatcc cggcaattct atatttattc tgacaattat
      421 tgcgattatt gtcattgggg caatatttgt gtatattttg aataaacccg caggttcaga
      481 aaccggagat aacctatccc cgggtatatg taaatcaggc aaggactgtt caccgcaaat
      541 cactcaaaat tattccaaag aaaatcttac ggtctatgag aaaaaaatgt caacagatct
      601 gcttcagctg attggcattg tgaagttacc ttccggaatg aaccaggatg cgtttgaaca
      661 ccagatgaaa caagcgcaac agcttaaatg gattgatgtc aacggaaaga caaccaatga
      721 taccatatcc gggcgaaaag ttgtgcatgt ttacgtaaag accctgaaaa attctgatcc
      781 aaatcttgtc gtcccctata cgtggaatgt ttcagattcg gatccatcca acaacatcat
      841 tgtggcctgg gtagaaccgg ataatcttcc aaaactcgca tctctcgatg atgtccagtc
      901 gatatggact gtgttacaac cgataaataa ctaacatagt gaatgaaaat atcgtaaggg
      961 aaaacaagac ggtgaacgat gagagagcac ataattgcca tttgtaccat tattgttatg
     1021 gcaggcctgc tgattattcc cgtgacagcg agtctgcatg agagttatgg tgagggaaaa
     1081 aatatcactt ttttgatcag ttccggaaaa ataaattcac cggatgtttc tattgacaaa
     1141 aattatctta cggaaagcga gagaaaactc tcctcaacgc ttctgaaaca gactttgaag
     1201 cagacttcgg catccagtct tgctacccct tcccagatta acggttcttt tgaatcttcg
     1261 ttgcgcataa agaatgtacc ggtaccttcc ggatccgacg gatcgccgaa ggaatccccc
     1321 aacctgatat caggaaaact ggtatatgcg tatgtttatg tgaaaccggg atactctacg
     1381 catatcattg attcatccgt gtttgaagta tcaggccggg atgaaaatta tcacagcgcg
     1441 gttgcctgga ttgatgttca ggcactcgaa cccttatctt cccttgaagg cgtacgaact
     1501 atccgggagg ttatacctcc catggtaaat accggatcgg ttaccacggc aggggacaca
     1561 attcataaaa cagccacggt ccggtcaacc tatggaaacc ggggtgccgg tatgaaaatc
     1621 gggattatct cagatggggt ggacaacctc gcctccgcgg tggctagtgg tgatcttcct
     1681 tctgatgtga cggtactgag caataccgag ggtggcgatg aagggacggt gatgctggaa
     1741 atcgttcatg acatggttcc cgacgcggaa ttgtatttcc atgatcaggg agatagcagc
     1801 attgcattca attcggcaat cgatgatctc gttgcaaatg ggtgcacggt tatttgtgat
     1861 gatgtcagct tgttcgctga accctatttc gaagatggaa atgttgcatc acatatcaag
     1921 acgctgctgg cgggtaacaa tttagtctat atatcttcag ccggcaacga tggatcaacc
     1981 aatacagctg caacccgtca tcattaccag gggtcgtatt atgaatccga gacgacgaac
     2041 tggaacgatt tcagccaggg atcatcatca acccccagcc tctatttaaa tttgtcaagc
     2101 gggagcaccg taacagtaat tctgcaatgg gatgacccgt ggggacactc cggtaacgac
     2161 tatgatttgt atctgcatgc aatgaatggg agtataataa catcttcgca attggttcag
     2221 tcgggtacca gtgatccaat cgaaatatta tcatatactc actcaggacc cataacggac
     2281 gcacaaatta acgtatttaa ctacaatggt ctcgctcaat cgagaaacct tgaactctac
     2341 attattaccg ggggttcaac actggtttac accaataata ttaattgcac agattccatc
     2401 ttcggccaac cggcagttac agatgtgatc gctgttgccg cagttcctgc cagttcacct
     2461 gcaacaattg aatcgttttc ttcacgtggt ccggtgacca tttcctatcc ttcccctgaa
     2521 acccggatta aaccggatat ttccggtgtt gactgcgtcg cggtcaccgg ggttggaggc
     2581 ttcggttcac cgttttgtgg tacaagtgca tccgcccccc atattgcagc agtagtagca
     2641 caatactggg gtgctcatcc gggcctgact ccagcccagg tacggactgc cctctacaca
     2701 aacgcagctg atcttggtac ttctggcaaa gatacggtat tcgggtatgg attagctgat
     2761 gcgttgaata tggaatctca ttttagcggg gggagtggtg gtagtatcgc agttgttgaa
     2821 aaatcattcc acttcggaac gtcgggggat attccggttg ccggtgactg gaatggagac
     2881 ggaatacagg atactggtgt gttccgccct tccaatggga actggtacct ggataccact
     2941 aaaaccgggg tcgtaaaccg gacattccat ttcggtacaa caggagatac tccagttact
     3001 ggcgactgga atggtgataa cgtctctgat gtcggggtct tcaggaactc gaatggtaac
     3061 tggtaccttg atactactaa aaccggggtc gtaaaccaga cattccgctt tggtaaaaat
     3121 ggagatacgc caattaccgg cgactggaat tccgatggta tatccgatat tggtgtcttc
     3181 aggaactcga atggtaactg gtatctggat actactaaaa ccggtgttgt aaaccggaca
     3241 ttcctctttg gtaaaaatgg agatatgcct gttatcggtg actggaattc cgatggtgta
     3301 tccgatattg gtgtcttcag gaactcgaat ggtaactggt atttaaatta cactcctcaa
     3361 atctgaactc taaaactcta aaattcaaag acccgggata aaaagaccaa gaaggtcaat
     3421 atttttttta aatatgggta aagtgctatt tttatccaaa attttaaaat aaaatcttta
     3481 gatcataaaa ctcgaatcaa ttactttgac gctcctgggg gcatcggtaa aacctctgcc
     3541 cccgccgctg tggcgatccc cacactgcga tagatccgca taaagttttc gcaatacgaa
     3601 aaagtatacc agcaaatcca tggttacttt tttttatctc agttttggtt atgaaaattt
     3661 ttcaaaaatc tcgtcggaga aataaaaaag attggggctg tattaacact ttaccctaaa
     3721 tattatacaa tggcatcttt catatttgcc gatcgtaaat ttaattcttt acaaaaccgc
     3781 cccgattaca atttttttga aggagaaata ccatgcagtg ttctaggacc gtctggaaat
     3841 atccgggctt ccttgctcac cactgtggtc gaagcaggat ccaacatcag attcttaata
     3901 gtaaattcct caaaaaacac ggtgtaatcc gctatctctt gcaataagga tagcaaaatg
     3961 atattccccc ggagtaattc ttcgccatag tgaagatggc agggaccctt tctcaattcc
     4021 tgtcgtatgc catgccgggt gatattgttc catgatattt acgtacgagt cgcatgatat
     4081 ttcctcggca atccagggca gtactttgtt actccctgca agattttccg gtaacacaag
     4141 gtgcctgatt atcatacccc gcaccgctat accttcatca ataactagat ctccaacttg
     4201 gcgatgcatt tcccttatcg ccgctttcat agtgatgaca taatccggag catgggataa
     4261 gagacgggca atctcctcgc tgccatattt agcgtcaggc atatagatat cgacaacatc
     4321 ctccagaagc atgagggtat caatggcatc gtaccccccg ctgttgtata caatcggaat
     4381 acaaagtccc tggtctgctg cgattgacag acttttaaga atctgcggaa caacgtggga
     4441 aggcgtgacc aggttgatat tatggcaacc ctgtcgttgc agggaaacca ttatctccgc
     4501 cagactctcg caggagattg tttttccggt ttcacattga ctgatatcat aattctggca
     4561 gtattcacag gaaagattgc agtgagcaaa aaaaacagta ccggatccat gacgccctac
     4621 aagagcagac tcttctccga aatgaggtcc aaaacttgaa atgaccggaa gaagaccggt
     4681 ctgacaaaat ccctgttcat cgttcaggcg gttaattctg catcttctcg ggcaaactgt
     4741 acaggatttc agcagataat atgccctctc tatccgcttt gcaagctcac cggattcatt
     4801 gagcgcaata tacgagggat attttgtccg gtcagtcttt gtataatcca ggatggacca
     4861 tccccgacag tttttatgaa tttaaacaca ctatcagatc tctttgacga tttcaccgtt
     4921 taatgcagtc acgtaccgta tggcgcaaag cgttgaggca atgatcgcat ctatacccag
     4981 ggtctcctca ctttcctgca cggcttttag tactgctgca gtagcgggca tgtgaggaac
     5041 tgcccggcat gcaagatcac cgggatgcgc atcaaaagtt ccaatcatgc gggcaagtgc
     5101 gaccgtctct tccttgtcat aggtaataag cgggcgtaac acgggttcct ttacggcttc
     5161 tgatatgacc ccaaggttgg ccagtgtctg ggaagccacc tgcccgagat tttctcccgt
     5221 aactatggca agtgcacctt catttcgcat aagctcacct gccaccctca acatgaagcg
     5281 tttgcagatc acgcagcggt accggggggg aattcgcagt ttgtccatgc agtcatataa
     5341 caattcgctg tttgcgatca caagagacag aggatttccg gcacaccagc gcgagagccg
     5401 gcggtggttc tcgatagctg cccgggtgac atccgctcct gcccaccttc ctgcatccag
     5461 atgcaggttt gtgatctcgc atccgcgttt catcatcagc catgaagcaa tgggtgaatc
     5521 aattcctgac gagagtaatg atagtacccg gccctgggta ccccacggta accccccggg
     5581 tgccggtatc cgtgaatcat acaccagccc gccaaaatca cggatttcga cgaaaagttc
     5641 ataatccggg ttattgaggt ccacgcgaag gccggggata tggtcataga cagcggatcc
     5701 tatgaccgtc ccgagctcct ggctgttcag gccggttttc tgttgccgtt ttgccctgac
     5761 tgcaaaactc atgccccgga ccaggtttgc agatgcgagg gaaatcgcaa ccgtacagat
     5821 atcttcgagc ccgcttgctg tccttgtaca gatacttaca tccacaatcc cgaaaatccg
     5881 ggataccact tcagcgatcc tttcaggctc atcgccgtaa atgaggatgc gcccccgggg
     5941 ggtttcgtaa tgataggaaa gagaagaagc ggtgagtgcc tttcccatat tgcgcagaag
     6001 cattccgata aaatgatgct tgactgactc gctttttaag aataactcgc catatctgac
     6061 cataacgagt ttttcctgac cgctcattcc gctcactccc gttcttaaga actattgaaa
     6121 gtcaagggaa ataaccattg gttgtgggaa tctgtagtgt cttttccact cgggacggcc
     6181 aaaagagtta tgtatggatg ctggaaaacg aaatccttta attttgcaaa cgaatagtgt
     6241 aatgaaatgg caaaaaaggc tagcaaaaaa attgaagctg aaccgctcag gttgttttat
     6301 attttttata atcaggagcg ctgggacaac tggttaaaaa cactcagtga atcgagtttt
     6361 gaagcggatc ccaagagcga cgagcttccc gaaggattcc ggatactgga cggtttctcg
     6421 gtagatatca ctgtttctgt tttgaagatt ataaaactct accagaacaa acggtttagc
     6481 tttgaagagg ctgttgacaa actctcccag atagaaacca tcgtgatgtc agccgctcct
     6541 gaaggtgaac tcggggagat cgtcgagatc ctgcagctcc ccaaacttgt gctttttgcc
     6601 tcctgcaggt tgtaccttgc cggtggattc aataaggata taaaaactct cgtcaagaaa
     6661 gggagggaag atatacagaa ggacatggag caggcactgg aagcagctgc aaatatcggt
     6721 gcgagtgtca ttgacggggc ttcctgctgt ggaaaatatg tcaaagatga tattgaagag
     6781 acaaccctct ttgatgagtg gctcattgaa tgtgaacgga tgggtgaagc catggtctcg
     6841 ctgaaaaact ttgatgagtc aacaggagac gaggattgat tgctgatcgg gcacggtttc
     6901 gtcatgcaaa aaagttagaa aggcttgcag gatttcggct tcccgagctt gcattttccg
     6961 ggaccatgct cgaaaccctc gcttcgcgtc ttgactatga tgcgctcgat ttttccgtga
     7021 gggaacagat cctcgctttt tttgacgatt ttcttcgctg tgactgtaaa gaatcgccac
     7081 tatgcgggtg tcccgagaaa aaatttgcaa aaaaagtcat cgagctgagg gagaatggtc
     7141 tcgatcaccg ccagatcgct ggcttcatgc tggatgaata cgggatagaa atgtttccaa
     7201 cggatgtcct ctcttttcta gaagaatctg ttcacgttct cgaagccatt tcagatgttg
     7261 cccgtttaca gggtagtgct gagctggcaa aaaaaaccga tgaacatatc cacctgattg
     7321 aacgataatc gggtactatc tttaaaacta ccggtgcgga aatcaggtac tggttttttt
     7381 aaaaaaggac gtttaggtct ataattcttt gaagtattaa tagtcctgac gccaacactc
     7441 actatggatt ttactccggt aaaaaaatgg gggatctttt ttcttgggat ac
//
LOCUS       DASRLV010000082         4687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_108874,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000082 DASRLV010000000
VERSION     DASRLV010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108874"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            298..948
                     /locus_tag="VFC43_03315"
     CDS             298..948
                     /locus_tag="VFC43_03315"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HZK30249.1"
                     /translation="MDRKLGTLVILLIICAIALSVAAHFFSVFPFDLKITRELKEEQN
                     PVVSQTLQWVSVLGETWIEAILVGTVIAICLVRHRWPEAVFVLATVSSVILTSVLKVL
                     VGRPRPPFFLGDPKDLFWSVDQFSFPSGHVLFYIVFFGFIAFLAWSHLTGYVRWLIMV
                     ICGLLIVLIGPSRVYLGAHWASDVIGSYVIGVLWLLMIILLYLRSGLSPVMQMKRE"
     gene            complement(1148..2185)
                     /locus_tag="VFC43_03320"
     CDS             complement(1148..2185)
                     /locus_tag="VFC43_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HZK30250.1"
                     /translation="MKLGLCRNVVDMPGSVAHMLIFEAALAEIDNQRDCDDLVSMLKE
                     KMNYGRLGSLGPDLPYFNGVLRNAASYLVAGYYQPEPIVQWGDLLHSKTPNLFPLTMI
                     EIAWRETDPDEEEWDVIAQNQWAFIMGYLTHVAADQMIHPYINKISGNYYRDKEKRKK
                     HMDCEIYQDVVAFNNRYKKSILDEHLDTWVNVHTKSGKTEPHFRRFLQKSFKETHAIY
                     PSENDIENWYRGLSFIQKWLKWIGLPYKQADADFRTSKENSKNYKEYCLNTPLAAGKS
                     YADYYNDAVELSSLYIRAANKLYEINHADFKDEFRKQFLTIVRNADLRNPLDNNILEE
                     AKSAYSAIYRA"
     gene            3268..4371
                     /locus_tag="VFC43_03325"
     CDS             3268..4371
                     /locus_tag="VFC43_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HZK30251.1"
                     /translation="MKILFVVCGEGLGHSSRCIHLGHFMQQQGYTVHYAGYGESLAFM
                     EQHKCSNLHTAHREVHLEGENGFFSLIKTLWYSKWFIPDMIRSALIVRQLIREYQFDC
                     VVCDTMYGGVYAARMEKTPVVFITNQNHFNSINGATNPVWQALNFLIGRYIRLANHVI
                     VPDYPAPNTISEYNIVVPRGEENRYTFTGPFFDIDREQYTFSKETIFTSFGGEPYKLP
                     MYKKLKTIADRRKDLYFDVFYTGPVLPDSSENFVSHGFVENIHEHLARAKIAIVHGGL
                     TTLHEAMAFGKPVLVIMDPNHPEQQNNAKKIVDVGAGTSVDGSTVTFEELETKIAETA
                     KCCPYPFCVTHPEVNGRKNATVIIRAAAERRQA"
BASE COUNT         1260 a         1033 c         1061 g         1333 t
ORIGIN      
        1 cccatactca ggctaaccac aatagcgggg gaaagtataa acatctcacc ggttgttgca
       61 aaatgtgttt caaaatctaa tttacggaaa aacatttata aaattttcct gaaataaaaa
      121 ccttcactgg gcaaggaaaa atacccttga ataattccct ggtggtaata agattttcat
      181 ggtttggggg tgaattcttc tggttcatgt gcgtttctac agagcagaaa ttttaacaca
      241 tccgcaaaaa agattacatc aaataaccgt gaatgggata atataaaaag acagagaatg
      301 gatcgaaaac tgggaacttt ggtaatcctg ctgatcatat gcgcaattgc actctcagtt
      361 gctgcacatt ttttttccgt tttcccattt gacctgaaga ttacccggga actcaaggaa
      421 gaacaaaacc cggtagtttc ccagaccctg caatgggtga gtgtacttgg tgaaacctgg
      481 atcgaagcta tactggtcgg aacagttatc gcgatctgtc tggtgcggca ccgatggcct
      541 gaagcggttt ttgtgcttgc caccgtaagc agcgtaatcc ttacttcagt tctgaaagtg
      601 cttgtcggcc gtccacggcc tcccttcttt ctgggagatc caaaagacct cttctggtcg
      661 gttgaccagt tcagctttcc gagcgggcat gtcctttttt atattgtctt cttcggtttc
      721 attgcctttc ttgcatggtc acaccttacc ggatacgtgc gatggctcat catggtgata
      781 tgcggtctcc tgatagtgct cattggccct tcccgtgttt atctcggggc ccactgggcg
      841 agcgatgtta tcgggagtta tgtaatcggc gtactatggc ttctcatgat aattctcctg
      901 tacctgcggt caggactcag tccggtgatg cagatgaaga gggaataaaa agacgacgag
      961 atcggtaaac aatgcatgga ggaaattatc aaagaattga agaatatatc tgaactgcca
     1021 tttcccccct cttcccggaa tgctgtttgt atttgccttg gggaatcgtg taaatcatga
     1081 gagccgttac gggtcaacaa atgaacgatc cgattcgttt taaaaaatgg taaacgatga
     1141 aaagatacta tgctctataa atggctgaat atgctgattt tgcttcttcc aggatattat
     1201 tatccagagg atttcgtaaa tcggcatttc gtacaatggt taaaaattgt ttccggaatt
     1261 cgtccttaaa atctgcatgg tttatttcat agagcttatt ggctgccctg atatagagtg
     1321 acgaaagttc gacagcatcg ttatagtaat ctgcatacga tttaccggct gccagcgggg
     1381 tattcaggca atattcttta taattttttg agttttcctt actggtccgg aaatctgcat
     1441 cagcttgttt atagggcaac ccgatccatt tcaaccattt ctggatgaat gacaaacccc
     1501 ggtaccagtt ttcaatatcg ttttctgacg ggtaaatagc atgtgtttcc ttgaacgatt
     1561 tttgcagaaa tctcctgaaa tgcggttcgg ttttcccgga ctttgtatgg acattcaccc
     1621 acgtatcaag gtgctcgtcg agaatacttt ttttgtaccg gttgttaaat gctaccacat
     1681 cctggtatat ctcgcaatcc atgtgttttt tccgtttttc cttatcccgg taatagtttc
     1741 ccgatatctt attgatatac ggatgaatca tctggtctgc ggcaacgtgc gttaagtatc
     1801 ccatgataaa cgcccattgg ttttgggcaa tgacatccca ttcctcctcg tccggatctg
     1861 tctccctcca ggcgatctca atcatcgtca acgggaagag gttgggtgtt tttgaatgca
     1921 gcagatcccc ccattgtacg atgggttccg gttgataata gccggctacc aggtaagaag
     1981 ccgcgtttct caggacgccg ttaaaatagg gcagatccgg gccaagcgaa ccaagtcgcc
     2041 cataattcat cttctctttg agcatgctga ccaggtcgtc gcaatccctt tgattatcga
     2101 tttcggccaa ggctgcctca aaaatgagca tatgagcaac ggaccccggc atgtcaacaa
     2161 cgttcctgca taatcccaat ttcatttctt attttaataa ttttgggtaa accggctccg
     2221 ggaaaaatcc ctgaaaagaa tttgatgaca ccccggaaac ggcaggcctc acggaacgtg
     2281 cggtgaggcg ggagaagcga tcccggttga aggttcgaat tgcggtaagt attgccccgg
     2341 agaaccctga taagctcttc tcgtccttat ggtcgggtaa aaggtatcga cgaaaagaag
     2401 ggaggcatga gctgattttc ggagtccgga tgatctattc ggtgctcata cctgctctgc
     2461 cgcatgaggc gttgaaaaga ctggcccgga tattattttc agaagattga ataaaaaaga
     2521 agaaaaatct gctctgtaga aacacatgaa caggagttcg cacccgaagc atgaaaatta
     2581 aaattccaga ctttcttttg atatgaattg ttaacccaac ttagttaatt ttcatgggcg
     2641 aggcgctggt taccggaccc aggtcaccaa cgcgggtttt ttgtcatggg cttccggtgt
     2701 ctcattcggg tacccgaata cgatcgtccc cacaggactg tacccatcag gaatttacgt
     2761 gctttcagag cgtctccgaa ctttctttta agaacggaga aaacattttc gaccttgttt
     2821 ctttgatggt agcagtcttg gtcgaaaaac ctagcaagtt ttcgtcgata gacaccgaat
     2881 atccgttttc gttttcttgt tctgaccgga ataagcgagt aagcgttcac gccgtcacgg
     2941 atgagtctgt gaatatcttc agaatcgtac ctttatcgag aatgtaggta tcagaccttc
     3001 ttgttcggtg acattgaacg agaagttttt cggcatgagg gatatcgtga actggttgct
     3061 gagagatttt gaacccggtg atgatctgtt ggtcagtttt caacggagaa cgatgttttc
     3121 aggaaccgtt ttcgagttca gataaaggaa cacgcaggaa gtatgattgt cgtaaccttc
     3181 acaggttctt acaattcatc gggaaagttt tcgccagagc agaaatttca tctttcatca
     3241 gatcatattt tttaaaacgg aacctgaatg aaaattcttt ttgttgtctg tggcgaagga
     3301 cttggacatt cgtcccgatg tatccacctc ggtcatttca tgcaacagca gggttatacc
     3361 gtccattatg ccgggtacgg ggaatcatta gcttttatgg aacagcacaa gtgcagtaac
     3421 ctgcacacgg cacaccgcga agtgcatctt gaaggagaga atggtttttt cagcctgata
     3481 aagacgctct ggtactcaaa atggtttatt cctgatatga tacgatctgc cctgatcgtg
     3541 agacagctca taagagaata ccagttcgat tgtgttgtgt gcgacaccat gtatggcggt
     3601 gtgtatgcag caaggatgga aaagacacct gtggttttta tcacaaacca gaaccatttc
     3661 aacagcatta acggggcaac caacccggtc tggcaggccc tcaattttct tattggcagg
     3721 tatatccggt tagccaacca tgtaatcgtc cccgattacc cggcacccaa tacgatcagt
     3781 gaatacaata ttgtggtacc gcgtggcgaa gaaaacaggt ataccttcac gggtcctttt
     3841 tttgatatag accgtgaaca gtacaccttt tcaaaggaaa cgatttttac cagttttggg
     3901 ggggaaccct ataaactccc aatgtacaag aagttaaaaa ctattgctga ccgtcgcaaa
     3961 gacctctatt ttgatgtctt ttataccggg cctgttctgc cggattcttc tgaaaatttt
     4021 gtgtctcacg ggtttgtaga gaatattcat gaacatcttg caagggcaaa aattgccatt
     4081 gtgcacggcg gacttacgac gttgcacgaa gcaatggcct ttggaaagcc agttctggtc
     4141 atcatggacc cgaaccaccc cgaacaacag aacaatgcaa aaaagatcgt tgatgtgggc
     4201 gcaggaacat ctgtcgatgg gagtacggtg accttcgagg aactggagac gaaaatcgca
     4261 gagactgcga aatgctgccc gtaccctttc tgtgtaactc accctgaagt caacggcaga
     4321 aaaaatgcaa ccgtaattat ccgtgcagca gctgagcgca ggcaagctta ataccgtgac
     4381 ccggacgacg atcctttctg ttttctctag aaaaatagct tcacggtcga acgataaaaa
     4441 taagcccaat acaggctctg atttttattt tgatatcccg gcccctcacc aaaagccttc
     4501 caaaagcctt taaaacgagg ctgtttttgc cacgtagttt atccaatcct gaccccccta
     4561 aaggagcccg aatgcaaata taaagatcat ttttaccaag tgagggcctc aataatgttt
     4621 caacttttga aaagtaaccc agatcttatt ttgattgaat cggggttata atataccatg
     4681 gcaaaaa
//
LOCUS       DASRLV010000083         5162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_916340,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000083 DASRLV010000000
VERSION     DASRLV010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916340"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="VFC43_03330"
     CDS             complement(<1..1230)
                     /locus_tag="VFC43_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HZK30252.1"
                     /translation="MEEKLICARNGTKEWNSEAMKQPLTFNLGLREYLTTVRDQPKPL
                     IIPLCAEMPLPAVSPLEVYTSIRKGSGFLLESMEGSEKIARYSFIGIDPEFVITIGKT
                     MDIKGNEPFTAIAKDPAGNTAVEQVRTILSRFCYLNVKAPRFFGGMVGYFAYDCVFSL
                     FEKVRATRDNSRASTGESSPGENSARFMFTKDCIVFDHAERKLYIFSSPFLTYESDLE
                     SEYHKSCSKILALRDYIAILGSDNRDRIPQNEDPNKKTGEYFTTVSKEKFEESVRSIK
                     EHIAAGDIFQAVLSRSIECAVNSDPFLIYAAIRKINPSPYMYYLDFGDEQVIGASPEM
                     LVRVEKRQVTTVPIAGTRPRGQDSGKDAAFARELLADPKERAEHTMLVDLARNDLGRV
                     CRFGTVEVEEFMGIEKFS"
     gene            complement(1485..1856)
                     /locus_tag="VFC43_03335"
     CDS             complement(1485..1856)
                     /locus_tag="VFC43_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30253.1"
                     /translation="MQVNYKRYGLYLLRWQASTPILAGVGILLVNMGQIVAAIVANLI
                     GGLIFFWVDQFIFTSQSLASQWEVRDNISCVDCGKIARGYRLVRSGDYDRTQDNAPEF
                     RCEDCSKKKAEELKGRGIKID"
     gene            2015..2575
                     /locus_tag="VFC43_03340"
     CDS             2015..2575
                     /locus_tag="VFC43_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30254.1"
                     /translation="MIGIIIFLIIVVLANLLTHYVSNSFYNSCVTFLNENFWLLIIIA
                     FILLAGDIFGVFAFPLNLPSPIIKAIGSVFFIFFILSLIRWVDTIAFTNFYFPFLFIS
                     YLLAVLVFLVVLISGYYEILRELSWQPEMEGEYGNDTYIEYSEYHDSAANPVTDAKSW
                     DEIGGEFRMMVYDIIHRFREDIRKTK"
     gene            complement(2593..2826)
                     /locus_tag="VFC43_03345"
     CDS             complement(2593..2826)
                     /locus_tag="VFC43_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30255.1"
                     /translation="MALSWREARECALREGLPQVYHDCDARMYGACREGEKQGSFKEG
                     IFVEHRCICMPAHLSHEELEVKEKKFLSENPDW"
     gene            complement(2938..3105)
                     /locus_tag="VFC43_03350"
     CDS             complement(2938..3105)
                     /locus_tag="VFC43_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxC-x17-CxxC domain-containing protein"
                     /protein_id="HZK30256.1"
                     /translation="MERKSFGGPRNFGPREMTKTVCSDCGKECEVPFKPTEGRPVYCR
                     DCLPKHRKPRF"
     gene            complement(3205..4332)
                     /locus_tag="VFC43_03355"
     CDS             complement(3205..4332)
                     /locus_tag="VFC43_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04084 family radical SAM/SPASM
                     domain-containing protein"
                     /protein_id="HZK30257.1"
                     /translation="MYFHLILTDDCNLCCSYCRARVFENPDEREDTEPQIHIDPHLPP
                     ELNVNLDLLYGFLSRDPSPSLTFYGGEPLLRTDLIERIMREAPAQRFMIQTNGLLLDK
                     INPKIVNRFSTILVSIDGREELNDANRGKGTYRKVIENIKKICRNGYKHEMIARMTVT
                     ESTDIVGAVHWLSDNPDYSFSSIHWQLDANFSHDFSHRSFTRWVNDSYNPGIFRLVKD
                     WVDHMENTGEVLYWYPFLDPMEDLLLKRESGLRCGSGYANYSIMTDGNIAPCPVMLGM
                     SEYYVGHVRDADPQNLKIMRVNGECAHCRILSFCGGRCLYSNITKPWGEPERQVVCST
                     VENLHRSLTSQLPRVKNLLASKRIDISDFSHEKYNGCEIIP"
     gene            4452..>5162
                     /locus_tag="VFC43_03360"
     CDS             4452..>5162
                     /locus_tag="VFC43_03360"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZK30258.1"
                     /translation="MVSQKPSSSFTWNPADYHKSSSSQKLWARELIEKLGFFGNERVI
                     DIGCGDGSVTAGIASRLPHGAVTGIDSSRGMIRFARKQFPASSYPNLSFVCMDARNLS
                     FTEEFDVAFSNAALHWITDHRPVLEGIARALCPGGTVLIQMGGKGNADQVFCVLNILV
                     EKQRWRRYFEEFSFTYGFFGIDEYRGWLEDAGLEPVRVELIPKDMSYQKREDFAGWIR
                     TTWLPWMSQLPEREKPAFI"
BASE COUNT         1318 a         1232 c         1139 g         1473 t
ORIGIN      
        1 ggaaaatttc tcgatgccca tgaattcttc aacttcaacc gtcccgaacc ggcatacccg
       61 tcccagatcg tttcttgcaa gatcaacgag catcgtatgc tcagctctct ccttgggatc
      121 tgccagaagt tcacgggcaa aggctgcatc ctttccggaa tcctgtcccc tcgggcgtgt
      181 gccggcgata ggcactgttg ttacctgacg tttctccact cgcacgagca tctccgggct
      241 cgcccctatc acctgctcat ccccaaaatc gaggtagtac atatacggac tggggttgat
      301 tttacggatc gccgcataga tcaaaaatgg atcactgttg accgcacact ctatgctgcg
      361 tgatagaact gcctggaaaa tgtcccccgc ggcaatgtgc tctttgatgc tccttacgga
      421 ttcctcaaat ttttcctttg atactgtagt aaaatattcc cccgtttttt tattcgggtc
      481 ctcattttgt ggtattctat ctcgattgtc cgaaccaagg atcgctatat aatcccgaag
      541 tgccagaatt tttgaacagc tcttatgata ttcggattca aggtcggact cgtacgtcag
      601 gaacgggctt gagaaaatgt acagtttccg ttcagcgtga tcaaagacaa tacagtcctt
      661 ggtaaacatg aaacgggctg aattttcccc cgggctgctt tcccctgtcg aagcccggct
      721 gttgtcccgg gtggcacgga ctttttcaaa aagggaaaaa acgcaatcgt acgcgaaata
      781 tccgaccata cccccgaaaa aacggggtgc ttttacattc aggtaacaga accgtgaaag
      841 aattgttctg acctgctcga cggccgtatt tcctgccgga tctttggcaa ttgcagtaaa
      901 cggttcattt cccttaatat ccatggtttt tcctatcgtg atgacaaact ccggatcgat
      961 cccgataaat gagtaccgtg caattttttc gctgccttcc atggactcga gaagaaaacc
     1021 actccctttt cttatgcttg tataaacctc aaggggtgag acagccggta aaggcatctc
     1081 agcacaaagc gggatgatca atggttttgg ctggtctcta actgttgtca ggtattcccg
     1141 gaggccaagg ttaaaggtga gtggctgttt catagcttct gaattccatt ctttcgttcc
     1201 attcctggca caaatcagtt tttcttccaa tccaccagca ccattcatgc aggaaaaaaa
     1261 cgtgcctacc cgaccgtgtg gtgttatttc tggtacacca ttaaactttg tacatattta
     1321 tacattgttg tatttatatg accgttggtg tattttattg cacctacaac agaaaaatac
     1381 gcaaaaaaag cagttttttt gaattaaggt aagaaaatag acgaattaat agtactcgga
     1441 caaatcgctt ttacctgatg attgtttttg ctttttgcct gcggctaatc tatttttatt
     1501 ccccgtcctt tcaattcttc tgctttcttt tttgaacagt cctcgcaccg gaattcaggt
     1561 gcattgtcct gtgtcctgtc gtaatctccc gatctgacaa gacggtaccc cctcgcgatc
     1621 tttccacaat ccacgcagga aatattatcc ctgacttccc actgggatgc tagtgactgg
     1681 gacgtaaaga taaactggtc cacccagaaa aagatcaacc ctcctatcag gtttgccacg
     1741 attgctgcga cgatctgccc catatttact aacaggatcc cgaccccggc aagaattggc
     1801 gtacttgcct gccatcgaag aaggtagaga ccataccgtt tataattgac ctgcatacca
     1861 gaagatccac gaaccggata ataggttttg tgatagcaaa aaagcatata aggtaatcta
     1921 tatatcaacc tgtacaaatt ttgtaatgca tttttgaggc agccacatgg gacaatacag
     1981 gagaaaacca ctgagctgga tttttttatc acggatgata gggattatca tcttcttgat
     2041 tattgtcgtc cttgctaatc tcctgaccca ttatgtgtca aattcatttt ataattcctg
     2101 tgttacgttc ctgaatgaaa atttctggct gctgataatt atcgctttca tcctcctggc
     2161 gggggacatc ttcggtgttt ttgccttccc gctcaacctt ccctccccta ttatcaaagc
     2221 aatagggagt gtattcttta ttttcttcat tctcagcctg atccgatggg tggataccat
     2281 tgccttcaca aatttctatt ttcccttcct gtttatctcg tatctgcttg cagttctggt
     2341 ttttctcgtg gttcttatca gcggctatta cgaaatactg cgggagctct cgtggcaacc
     2401 cgaaatggag ggtgaatatg gcaacgatac ctacatcgaa tacagcgaat atcatgattc
     2461 ggctgccaac ccggtaactg atgcgaaatc ctgggatgaa atcggaggag aatttcgtat
     2521 gatggtatat gacatcattc accggttccg tgaagatatc cggaaaacaa aataaaacga
     2581 tagtaagggg aatcaccagt ccgggttttc cgaaagaaat ttcttttctt ttacttcgag
     2641 ttcttcgtga ctcaaatgtg caggcataca gatgcagcgg tgctctacaa agatcccctc
     2701 tttgaacgat ccctgtttct ccccctccct gcatgctcca tacatccggg catcgcagtc
     2761 atgatacacc tgagggagcc cttcccgcag agcacattcc cttgcctctc tccatgatag
     2821 tgccatagtg gcagtatagg tcagtatata aaaaagagtc tttcgattcg ttcaaaaatt
     2881 ttttgatggc ttttgctgcc ggatatgtcg ctaaaaaaaa gaaaaaatgt aatgaattca
     2941 gaaccggggt ttccggtgtt tcgggaggca gtctctgcaa tagaccggcc tgccctctgt
     3001 tggcttgaag gggacttcgc attcttttcc acagtctgag cagactgtct ttgtcatttc
     3061 tctgggaccg aagtttctcg gaccaccaaa actcttcctt tccattgtat tactcatgca
     3121 gcattagatc tgcgtataac tatttttttc aggattataa atacatctgc agggtggcgg
     3181 agagaaaaca ctatttacaa gcgattaagg aatgatctcg catccattgt atttttcatg
     3241 ggaaaaatcg gaaatatcta tcctcttgga tgccagaaga tttttcacac gcggaagctg
     3301 actggtcagg cttctatgaa gattttctac ggtactgcaa accacctggc gctcaggttc
     3361 tccccacggt tttgtgatat ttgaatagag gcatctcccg ccgcagaagg aaaggatccg
     3421 gcagtgtgca cattccccat ttactctcat aattttgaga ttttgagggt cagcgtctct
     3481 cacatgcccg acataatatt ctgacatccc gagcattacc gggcagggtg caatatttcc
     3541 gtcagtcata atgctataat tggcatagcc ggacccgcac cggagcccgc tctctcgttt
     3601 taaaagaagg tcctccatag gatcaagaaa cggataccag tacagcactt cccccgtgtt
     3661 ttccatatga tcaacccagt cctttacgag cctgaaaata cccggattgt agctgtcatt
     3721 cacccagcgt gtaaaactgc gatgtgaaaa atcatgggaa aaatttgcat caagctgcca
     3781 gtgaatagaa gaaaacgaat aatccggatt atcagaaagc cagtggaccg ctccaacgat
     3841 atctgtactc tcagttaccg tcatacgtgc tatcatttcg tgcttatatc cattccggca
     3901 gatcttttta atattctcaa ttaccttgcg gtacgttccc ttaccccggt ttgcatcgtt
     3961 gagttcctcc cggccgtcaa tagatacaag gattgtagag aaccggttaa caatttttgg
     4021 attaattttg tccagaagga ggccattggt ctggatcatg aaacgctgcg caggcgcctc
     4081 cctcataatc ctctcaatca gatccgttcg caaaaggggt tccccgccgt aaaatgtgag
     4141 agagggggaa gggtccctgc taaggaaacc atagagcaga tccagattaa cgttcagttc
     4201 tggcggaaga tggggatcga tgtggatctg tggttctgta tcttcgcgct catcaggatt
     4261 ctcaaacact ctggcccggc agtagctgca acaaagattg caatcatcgg tgaggatgag
     4321 gtggaagtac aagaaaatct catcataatg ggattttcca gacgataaaa ttaagggatg
     4381 tgtcaggtca ctttttcctt gaatcccctc ttttaaatta tatcccaata tatagttacg
     4441 agaacatctc catggtttca caaaaacctt catcatcatt tacctggaat cctgccgatt
     4501 accacaagag ctcttcctcc cagaagttat gggcacggga actcatcgaa aaactcggat
     4561 ttttcgggaa tgaacgggtg atcgatatcg gctgtggtga tgggagcgtg actgccggga
     4621 tagcctcgag actgcctcac ggagcagtga caggtattga cagttccaga gggatgatcc
     4681 ggtttgccag gaaacagttt cccgccagtt cttatccgaa cctttcgttt gtgtgcatgg
     4741 atgcccgaaa tctttccttt acagaagagt tcgatgtagc attttcaaat gcagcactcc
     4801 actggatcac ggatcaccgg ccagtactcg aaggaattgc acgggccttg tgtcctggcg
     4861 gaacggtatt gatccagatg ggtggaaaag gtaatgctga tcaggttttc tgcgtgctca
     4921 atatactggt ggagaaacaa cgctggcggc gatattttga agagttctcg ttcacgtatg
     4981 gtttttttgg tatagatgag taccgagggt ggcttgaaga tgcgggtctt gaacccgtcc
     5041 gcgtagagtt gatcccaaag gacatgtcat accagaaacg ggaggacttt gcaggctgga
     5101 tacgcacaac gtggcttccc tggatgtcgc agttgccaga gcgggaaaaa ccggcgttta
     5161 tc
//
LOCUS       DASRLV010000084         4433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_349771,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000084 DASRLV010000000
VERSION     DASRLV010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349771"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..205
                     /locus_tag="VFC43_03365"
                     /pseudo
     CDS             71..205
                     /locus_tag="VFC43_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868206.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
     gene            complement(395..1696)
                     /locus_tag="VFC43_03370"
     CDS             complement(395..1696)
                     /locus_tag="VFC43_03370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK30259.1"
                     /translation="MASVIATVAITVISLKNGIFDVFPYIYLVPVVLIAFAHPKYSVY
                     ATVLVGWLYLGLVFFIGLPDSQRYTLATIWFYVFVSIGVLISIYSQVYLREGQKSIAA
                     FDNSQAGVFCFDKESLRLKAVNQKFSYIIRYGQDDLLKKALPEIIPDAAERESFLASI
                     HDKKRIGDIEVRFRTWDGSMRWALVSATVSDGPDIICTLVDITDHKQAQESLTVANKK
                     LNLLNNVTRHDVLNQLSALLGYIDLSRHETKDIRMLSYIEKEEQAANTIRSQILFTRD
                     YQNIGIHNPEWHNIAETVSLAMASLDLQHVHITVDHSSVEIYADPLFERVFYNLVENS
                     IRHGEGITRIGIHLEQTVDGIDLIVEDDGPGIPEKEKENIFRRKYFKNTGFGLFLSRE
                     ILSITSLTIRENGIPGEGAHFVIHAPKGTYREGTAPTASPV"
     gene            complement(1970..2524)
                     /locus_tag="VFC43_03375"
     CDS             complement(1970..2524)
                     /locus_tag="VFC43_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 domain-containing protein"
                     /protein_id="HZK30260.1"
                     /translation="MGLLKSGINEVIATTRFNAAPMGIHYREGKASMVVFLGSNTAEN
                     IERDGWVVANFVHDPVVYVRTAFEDLPQNSFIEEPVAQKTVMRLAGADAWAAFTATIA
                     RKTTETMMVHLSLEKEIIEGVVLQPVNRGFNNIIEATVHATRYKENRDPELKKQIEYH
                     TGIVRKCGGKRETEALDLLTRFIQ"
     gene            complement(2512..3333)
                     /locus_tag="VFC43_03380"
     CDS             complement(2512..3333)
                     /locus_tag="VFC43_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HZK30261.1"
                     /translation="MTCLLMRAAERAQLAMMLEVCAYPKPGNVDRCHDYPDTRLEHFL
                     ASTLFARQAFEEAETGTGRIGEIIKHAVRDTNCHEGGNTHFGAFILLVPLIYGKGIPG
                     ALKAIEKTDVSDAVAFYKAFAMTSVKMNPADELDVHDPHTLTVIRDQEMTLLDIMQHS
                     ATNDMVAREWVTGFPLTRRGADLLKHYGPGRQSIVDTFLTLLASEPDTFIIKKHGSAA
                     AQEIMERARDVLDGRQRLEDLDADCIERGINPGSTADIIIASLFIALGEGWKWDY"
     gene            complement(3305..3601)
                     /locus_tag="VFC43_03385"
     CDS             complement(3305..3601)
                     /locus_tag="VFC43_03385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 9 domain-containing
                     protein"
                     /protein_id="HZK30262.1"
                     /translation="MPVKECPLIPALSRMDLPKEEYIRIKMDAVKGTPLQEGNQTCFG
                     SLTWCCKTSSPCMFRDMTLQQRGLSKKEYMRLKRELSDTIMSRVFHDVPSDESS"
     gene            complement(3511..>4433)
                     /locus_tag="VFC43_03390"
     CDS             complement(3511..>4433)
                     /locus_tag="VFC43_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HZK30263.1"
                     /translation="FERFGLLINDRIVRTPVAVASMAGWVDAAYILERADHIGAAFIG
                     GYSIDKPTMDAAKIISDEGNRKEFLYDDPVTELKKQMALLAKSKVVLGLNLRGSSPAS
                     FSAIAGSLGDQVVYEIDAHCRQPAMVAAGCGEHYLKKPGELMDVVRALKSDGVTVSVK
                     IRAGIAADDRLLARKLWESGADILHIDLMDFGSAKIRQIRNSCPLMIVANNSINTFER
                     MREMLSHGADLVSLARKSDIRTLSGLDAAITRYATEEGWYNSPKQLCRGGIYGPLLFA
                     ACRSRSARSSLHYRAWICQKKNIYGLKWMR"
BASE COUNT         1138 a         1086 c         1014 g         1195 t
ORIGIN      
        1 atccataata acgtgggaac cacttgtaac ggtcccgaca acagctgcat ctctgccgta
       61 ggtatggcac gcccatacct acggcagaga tgcagctgtt gtcgggaccg ttacaagtgg
      121 ttcccacgtt attatggata cactggttgg tggcgagcgg tatattgaac cgcccatagg
      181 tgatcctgta ccccgtgtct gctgagtata cttgaaaacg ggtatttttc agattcggat
      241 gattttttta gacgatttcc tggcagattc atgtaagtgt aatcacataa aggctgaaaa
      301 aggccttgtg aagagttcca gacgagagaa gaatagcagg agtaaaaacc ttctcaatta
      361 ttccgggtat tttttacatc tgacccggta tttttcatac cggagaagcg gtaggtgccg
      421 taccttccct gtacgttccc ttcggagcat ggatcacgaa atgggcaccc tcccccggta
      481 tgccattctc acggatagta aggctggtga ttgagaggat ctccctgctt aaaaacagac
      541 cgaaaccggt atttttgaaa tatttccttc ggaagatgtt ctctttttcc ttttccggta
      601 ttcccggacc atcatcttcc acgatgaggt caataccgtc aacagtctgc tccagatgga
      661 ttcctatcct ggttattcct tccccatgac ggattgaatt ctcaaccaga ttataaaata
      721 ctctttcaaa aaggggatca gcatagatct cgacagatga atgatctacc gtaatatgta
      781 catgctggag gtcaagggac gccatggcaa gtgatacggt ctcggcgata ttgtgccact
      841 cgggattatg gataccaata ttctgataat cgcgggtaaa caggatctgg ctacggatcg
      901 tatttgcggc ctgctcttct ttttcaatat aggagagcat cctaatgtct tttgtttcat
      961 gacgcgacag gtcaatataa ccaagaaggg cagatagctg attgagtaca tcatgccggg
     1021 ttacgttatt gagcagattg agttttttgt ttgccacggt gagagattcc tgtgcctgct
     1081 tatgatcggt gatgtcaaca agcgtgcaga taatgtcggg tccatctgat accgtcgcag
     1141 aaacgagcgc ccatctcata cttccatccc aggtccgaaa ccggacttcg atatccccaa
     1201 ttcttttttt atcatggata cttgccaaaa agctttcccg ttccgctgca tcagggataa
     1261 tctcgggaag agcttttttc aacaggtcat cctgcccata ccggataata tatgaaaatt
     1321 tctggttcac agccttgagc cttagggatt ctttatcgaa acaaaatact cctgcctggg
     1381 aattgtcaaa tgctgcaata ctcttttgtc cttctctgag ataaacctgt gaataaattg
     1441 agataagaac gcctatggaa acgaaaacgt agaaccatat ggttgccagc gtatagcgct
     1501 gggaatccgg cagcccgatg aaaaagacga gacccagata taaccagcca acgagcacgg
     1561 ttgcatagac actgtacttt gggtgtgcaa aggcgatgag gacaacggga accaggtaga
     1621 tataggggaa aacatcaaag attccgtttt taaggcttat gaccgtaatc gccactgtag
     1681 cgattacact cgccacaatg caaatatcaa aaaataaatt ttttattgga gaacgcggtt
     1741 gcataaagta ccatcacttt tgtaaaacaa tcactgctga tccatatttc agtaatcaac
     1801 agaggtgatc cttcgtaata aaggtataga agacggtttt ttggatccag aggagattat
     1861 tggagtattt aaccgggtga caggactttc tgagccttac ccaatatcga acaacaatta
     1921 ttatttttcg aatttacctc tgagtagagg gtaacaatcc ccagaggact tattggataa
     1981 accgggtcag caggtccagg gcttcagttt ccctttttcc cccgcacttg cggacaatgc
     2041 ccgtatgata ctcgatctgt ttttttaatt ccggatcccg gttctcttta tatcttgtag
     2101 catgcactgt ggcctcaata atattattga aaccccggtt taccggctgg agaacaacac
     2161 cttcaataat ctccttttca agcgagagat gaaccatcat cgtctcggta gttttgcggg
     2221 cgatggtagc agtgaatgcg gcccatgcat cagcacctgc aagcctcatg accgtctttt
     2281 gtgcaaccgg ttcttcaata aatgaatttt gtggcagatc ttcaaaagct gtccttacat
     2341 agactacggg atcatgtaca aaatttgcaa caacccagcc atcgcgctca atattttctg
     2401 cagtgttgct ccctaaaaaa acaaccatgc tggcttttcc ttcccggtaa tggatgccca
     2461 tgggtgctgc attaaaacgg gtggttgcta tgacttcatt tatcccgctt ttcaatagtc
     2521 ccatttccat ccctccccga gcgcaataaa gagagaggct atgatgatat ctgctgttga
     2581 acccgggttg atacctcgtt caatacaatc ggcatcaaga tcctctaacc tctgtctgcc
     2641 gtcaaggaca tcgcgggccc tctccatgat ttcctgcgct gctgcagagc cgtgtttttt
     2701 gataataaag gtatccggtt cagaggcaag cagggtgaga aatgtatcga ctatggactg
     2761 ccggcccggg ccgtagtgtt taaggagatc agccccccgc cgtgtaaggg gaaaccctgt
     2821 cacccactcc cgcgctacca tatcattggt cgccgaatgc tgcataatgt ccagaagtgt
     2881 catctcctgg tcccggatca ccgtaagagt atggggatcg tgcacatcca gctcatcggc
     2941 cggattcatc ttcactgagg tcatggcaaa ggccttgtaa aacgcgacag cgtcagatac
     3001 atcagtcttt tctatagctt tcagtgcacc gggaattcct ttgccgtaaa taaggggtac
     3061 gagaaggata aaagcgccaa aatgtgtatt ccctccttca tgacaattgg tatcacggac
     3121 ggcatgcttg attatttccc cgattcttcc cgtacccgtt tcagcctcct caaacgcctg
     3181 tcgcgcaaag agggttgacg caaggaaatg ttcaagacgg gtgtcaggat agtcatgaca
     3241 gcgatccacg ttcccgggtt taggatacgc acacacttca agcatcatcg caagctgtgc
     3301 gcgttcagct gctctcatca gaaggcacgt catggaaaac acggctcatt atcgtatctg
     3361 agagctcgcg tttcaggcgc atatactctt tctttgaaag accccgctgc tgaagcgtca
     3421 tatcgcggaa catgcagggt gatgatgttt tacagcacca ggtcaggctt ccaaaacagg
     3481 tctgatttcc ctcttgcagc ggagtacctt ttaccgcatc cattttaatc cgtatatatt
     3541 cttcttttgg cagatccatg cgcgataatg cagggatgag cgggcactcc ttgaccggca
     3601 tgcagcaaaa agcaagggcc cgtatatccc gccgcggcat aattgttttg gagaattata
     3661 ccagccttcc tcggtcgcgt agcgtgtaat tgccgcatca agtcccgata atgtacgtat
     3721 atcggatttg cgtgcaagtg acacgagatc tgccccatgg gagagcattt ctctcattcg
     3781 ctcaaaggta tttatcgaat tatttgcaac aatcataagc gggcagctgt tccgtatctg
     3841 gcggattttt gccgatccga agtccatgag atcgatatgc agaatatctg ctccggattc
     3901 ccacagtttg cgggcaagca accggtcgtc cgcagcgatt ccggcacgga tttttacaga
     3961 aacggtgact ccatcagatt tgagagcccg gacaacatcc attaattcgc caggtttttt
     4021 taagtagtgt tccccgcacc cggcagccac cattgcaggt tgtcggcaat gggcgtcaat
     4081 ttcgtacact acctggtcac ccagtgaacc tgcaatcgca ctgaacgagg cagggctact
     4141 tcccctgagg ttgagtccca gtacaacctt gctttttgcc aacagtgcca tctgcttttt
     4201 tagttctgta accgggtcat cgtataaaaa ttccttgcga ttaccttcat cagaaatgat
     4261 cttggcggca tccatggtgg gtttgtcaat cgagtaaccc ccgataaaag cagcccctat
     4321 atgatcagcg cgttcaagaa tgtatgccgc gtctacccat cccgccatgg atgcaactgc
     4381 aacaggtgtc ctgacaatcc ggtcgttaat gagcagccca aaccgctcaa agg
//
LOCUS       DASRLV010000085         4951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_430217,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000085 DASRLV010000000
VERSION     DASRLV010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430217"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VFC43_03395"
     CDS             <1..376
                     /locus_tag="VFC43_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30264.1"
                     /translation="WYSMDAYDIDDLINNYSDFEKSIDDKILALKKIKDHPDSANTAL
                     MIQLIESQKRSAEQAGKILFFSRLTGILAGGALIIALGTLYLVYSAVGPEKIFWGTLT
                     IVAAGAALMGLLLLAWPGQKLR"
     gene            424..774
                     /locus_tag="VFC43_03400"
     CDS             424..774
                     /locus_tag="VFC43_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HZK30265.1"
                     /translation="MTEQIKAARISEMKPGMMKKVMVMGRSILLASVNGRYFAVDAVC
                     PHLEGDLSSGTLNGTTLTCPMHNSQFDLRDGHVIRWTDQTGIRLEYASRAHPPRPLKH
                     YPVRIEGDTIFIEI"
     gene            complement(878..2140)
                     /locus_tag="VFC43_03405"
     CDS             complement(878..2140)
                     /locus_tag="VFC43_03405"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF002381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HZK30266.1"
                     /translation="MTELGVFSEVGKLREVMGHRPDLSLRRLTPANHNDFLFDDVLWV
                     DRAIMEHNAFVRLIHEEGVKVRYLQELLAETLAEGDDIRRQVIDHVSAGMTIGISSLD
                     AVKMCLMDMGAEDLATHLIGGLTVAELECIYTQGLARSSLTAAVNGADAFILPPLPNS
                     LFTRDSSSWVFNGVSVNPMYWPARRPESFNAGFIYRYHPKFRTQKFEFWYPLLDDEMM
                     VSLMDTGRTSLEGGDVMPIGNGTVLIGLSERTQAPMIERLAQSLFSKKAAERIIVAQM
                     SRDRAHMHLDTVFCMLDVDTVTVWPDVVNRMEAYSIRAGDDKAMFSVTKEESFLKAVA
                     DALDIDRLEVIPTGGDSYEAAREQWDDGNNILTLKPGVVLAMNRNAFTNRNFRKAGID
                     VLEFEGSELSRGRGGGHCMTCPLLRDPA"
     gene            complement(2157..3071)
                     /gene="arcC"
                     /locus_tag="VFC43_03410"
     CDS             complement(2157..3071)
                     /gene="arcC"
                     /locus_tag="VFC43_03410"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HZK30267.1"
                     /translation="MRIVIALGGNALLKRGEPMSAEMQRINIRIATHALAPLVQDHEL
                     VLSHGNGPQVGLLALQAADYTEVDPYPLDVLDAQTEGMIGYMIEQELRNLLPRDVPLA
                     TILTMIAIDPADPAFEHPTKFVGPQYPKADADRIAVEKGWFFALDGDAWRRVVPSPRP
                     QRIFEIRPIRWLLEQGAIVICAGGGGIPVMYQQDIERHLTGVEAVIDKDLASGLLAQE
                     IKADLFIMATDVEGVYYDWGKPSARLIRKTTPRELQRHVFPAGSMGPKVEAACTFVKE
                     TGKVAAIGALMDIEAIIAGDLGTIIRPD"
     gene            complement(3334..>4951)
                     /locus_tag="VFC43_03415"
     CDS             complement(3334..>4951)
                     /locus_tag="VFC43_03415"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HZK30268.1"
                     /translation="VYLFGHRQPDTDSIASVIGYAEFKNLSEPGRYIPARCGELNPES
                     RYVLKRFCLPDPVLLASVKPTLSDISYKPVFSLPRTVPAVDVATLMAKEGIRNIVITD
                     DCGKPVGMVGEHALAGTYTQAIPLHELAIMPLSADSLARILDATVHVSERAVLKGRVY
                     IVIDAPDVTLGRMSSLDIAVVGDNEPVQHALISAGIACLIVANGAPVRERIATAAKER
                     GVCLLSTPLDAFGVGRRINLSLPSREIMETDVPVLALSDTIAHARQVVSSSKFRAACV
                     VTADGTLAGILTRTTLLDDVRRSVILLDHNEASQSVDGIEEADIVEIIDHHRLGAITT
                     LKPIRFFNDPVGATSTIIAMKFMESGLTPSVPVAGALLAGILSDTLSLKMSTTTHRDM
                     QAVKFLARITGEDPERLGSCLIEQGMDLSGTSLDLILARDLKEFVLSKKKVLISQVMV
                     PSFAWNHERDDAIQAELGRMKTLQGADIVLALFTNVVEKSSELYAAADPGLLSAVFHS
                     DLPVRLEGVMSRKLDFLPWFGARLRNQTRQ"
BASE COUNT         1234 a         1319 c         1241 g         1157 t
ORIGIN      
        1 atggtatagt atggatgcat acgatatcga tgatttaatc aataattatt ctgattttga
       61 aaaatcgata gatgataaaa tactggcgtt aaaaaaaatc aaggatcatc cggatagtgc
      121 caataccgca ctcatgatac agttaatcga atcccagaag agatcagccg aacaggcagg
      181 aaaaatactt tttttctcac gactcacggg aattttggcc ggtggagcac tcatcatcgc
      241 cctgggtaca ttataccttg tctatagtgc tgtcgggccg gaaaaaatat tctggggcac
      301 cctgactatc gtagctgccg gtgcagcatt aatggggctt ctcctgctcg catggccagg
      361 acaaaaacta cgataaccaa aaagatttcg gaaaatatcc ccacacggac gcaactgata
      421 tccatgactg aacaaataaa agcagcccgt atttccgaaa tgaaacccgg aatgatgaaa
      481 aaagtcatgg tcatgggccg gagtattctt cttgcgtcgg taaatggcag gtatttcgcg
      541 gttgacgcgg tctgcccgca tcttgaggga gacctgtcat caggaacgtt aaacggaaca
      601 accctgacct gcccgatgca caactcccag ttcgatctca gggacggcca cgtcatccgg
      661 tggaccgacc agaccggaat ccgccttgag tatgcatccc gggctcatcc ccccaggccc
      721 ctgaagcact acccggtaag gattgagggg gatacaattt tcatagagat ttaaaaaccc
      781 gaatttttat taaaatgttc ctcccgcatg ctgtcagaaa ccctgttttt acgccggttg
      841 gtgagtatga actgggttga ccccagcccg tatgtgctca ggctggatca cggagcagag
      901 gacaggtcat gcaatgccct ccaccccttc ccctgctcaa ttctgatcct tcaaattcca
      961 ggacatcaat accagctttc ctgaaattcc tgttggtaaa cgcgttccgg ttcatggcga
     1021 gaacgacacc gggtttgaga gtaagtatat tgttcccgtc atcccactgc tcacgggctg
     1081 cttcatagga atcacccccg gtcgggatga cttccagccg gtcgatgtcg agggcatcag
     1141 caaccgcttt aagaaaactc tcttcctttg taacgctgaa cattgcctta tcatcgccgg
     1201 cacggatact gtacgcctcc attctgttca ccacatccgg ccaaacggtt accgtatcga
     1261 catcgagcat gcaaaagacc gtatccaggt gcatatgagc cctgtcccgg ctcatctgtg
     1321 caacaataat acgctctgct gctttttttg aaaaaaggga ttgtgccagt cgttcaatca
     1381 tgggtgcctg cgtcctctcg cttaaaccaa taaggacagt gccattgcca atcggcataa
     1441 catccccacc ctcaagtgat gtacgaccgg tatccatcag ggaaaccatc atttcatcat
     1501 ccaataaagg ataccagaac tcgaacttct gagtgcggaa tttgggatgg taccggtata
     1561 tgaatcccgc attgaaggat tcggggcgcc gggcaggcca gtacatcggg ttgacggaga
     1621 caccattaaa gacccaggat gaagaatcgc gggtaaagag tgaatttggc agtggaggca
     1681 ggataaaagc gtccgctccg ttcactgcag cagtgagcga ggaccgggcg agcccttgcg
     1741 tgtatatgca ttcgagttcg gcgaccgtaa gtcccccgat gaggtgtgtt gccagatcct
     1801 cagctcccat atccattaag cacattttta ctgcatcaag ggatgagata ccgatggtca
     1861 taccagctga aacatgatca atcacctgcc tccgtatatc atccccttcc gcaagtgtct
     1921 cagcgagcag ttcctgcagg tacctcacct taacaccttc ttcatggata aggcggacaa
     1981 acgcattgtg ttccatgatg gcccggtcaa cccagagcac atcatcgaac aggaaatcat
     2041 tatggttcgc cggtgtcagc cgccgcagac tgagatcagg gcggtgaccc atgacctcgc
     2101 gaagttttcc aacctcggaa aataccccta attctgtcat ttttcctcgc taaccattaa
     2161 tccggtcgaa ttattgttcc caaatcaccc gcaatgatcg cctcgatatc catgagagcc
     2221 cctatagcag cgactttacc ggtttctttg acgaatgtgc aggctgcttc cactttgggt
     2281 cccatggagc ctgcgggaaa cacgtggcgc tgtaattccc gcggcgtggt ctttcggata
     2341 aggcgggcag agggttttcc ccagtcataa tacactcctt ccacatcggt ggccatgatg
     2401 aagagatctg cttttatttc ctgcgcgaga agaccgcttg caagatcctt gtcaataaca
     2461 gcctccacac ccgtcaggtg tcgttcgatg tcctgctggt acataacggg tattccccct
     2521 ccccctgcac agattacaat tgccccctgc tcaaggagcc agcgaatcgg gcggatctca
     2581 aagatccgct gtggtcgggg cgaggggacg actcttctcc acgcatcacc atccagagca
     2641 aaaaaccagc ccttttcaac ggcaatccga tcagcatcgg cttttggata ctgaggaccg
     2701 acaaacttgg tcgggtgttc gaacgcggga tcggccggat caatcgcgat catggtaaga
     2761 atcgtggcaa ggggtacatc ccgcggcagg aggttcctga gttcctgttc tatcatatat
     2821 cctatcatcc cctcggtctg ggcatcaagg acatcaagag gatagggatc aacttcagta
     2881 taatccgctg cctgtaaagc gagcaggccg acctggggcc cattgccatg agagagcacg
     2941 agttcatggt cctgcacaag cggtgccagt gcatgcgtcg caatcctgat atttatccgc
     3001 tgcatttcag cagacattgg ttcccctcgt ttcaggaggg catttccccc aagggcgata
     3061 acaatcctca tctgcacccc gttattcctc tgagcatacg atcacgacct ccccctgtcc
     3121 gggctcctgg tttttcgttt ttatccagcc cggtccctgc tccgattgcc attacagcat
     3181 cgtacccttt tggaatattt ataggaacgg attttgtaac gactgttttt tgcgattgga
     3241 atgagcggta accggtataa aaatgaaata taagggcagg gtactacgtt accggagtat
     3301 tatgcttttt aaccaggact atgttaaaac aggtcattgc cgggtctgat tccggagccg
     3361 tgcgccaaac cagggaagga aatcaagttt ccgtgacatg acgccctcca gcctgaccgg
     3421 aagatcgctg tggaaaactg cggaaaggag cccgggatct gcagcagcat agagttcact
     3481 tgatttttcc accacattgg tgaacagggc aaggacaata tcagctccct gcagggtttt
     3541 catccggccc agctctgcct ggatcgcgtc atcccgttca tggttccagg caaaagaggg
     3601 gaccatcacc tgggatatca gcaccttttt tttagagagg acaaactcct tgagatcccg
     3661 cgcaagaatg aggtccaggg atgttcccga caggtccata ccctgttcga taaggcaact
     3721 gccgagccgt tccggatctt cccctgttat ccgggccaag aattttactg cctgcatatc
     3781 acggtgggtg gtggtcgaca ttttgaggga aagggtatcg gacaggatgc cggcaaggag
     3841 tgccccggca acaggtactg aaggagtcag ccccgattcc atgaatttca tggcgattat
     3901 cgttgatgtc gcacccacag gatcattgaa gaaccgtatc ggtttcaacg tagttatcgc
     3961 ccccagccgg tggtggtcaa tgatctcgac aatgtcggcc tcttcaatac cgtccactga
     4021 ctgggaagcc tcgttgtggt caaggaggat gaccgacctc cgtacatcgt ctaacagagt
     4081 agtccgtgtc aggatgccgg caagtgttcc gtctgcggtg acaacgcagg ccgcacggaa
     4141 ttttgatgat gagacaacct gtcgagcgtg agcgatcgta tcggaaagcg caagaaccgg
     4201 aacatccgtt tccatgattt cacgggaagg cagcgagagg ttgatccgcc gcccgacccc
     4261 aaaggcgtcg agaggagtcg agaggagaca tacaccccgt tcttttgcgg ccgtggcaat
     4321 gcgttccctg acaggagcgc catttgcaac aatgaggcag gcaatacccg cagagatcag
     4381 ggcatgctgt accggctcat tatcaccaac gacggcgata tccaaagatg acatcctgcc
     4441 aagcgtcaca tccggcgcat cgatgacgat atatacgcgg ccttttaaca ccgctcgttc
     4501 tgaaacatgg actgtcgcat ccagtatgcg ggcaagcgaa tctgctgaca gcggcattat
     4561 ggcaagttca tgaagaggga ttgcctgagt ataggtacca gccagagcgt gttcgccaac
     4621 cataccgacc ggttttccgc agtcatcggt gataacaatg ttccgaatcc cttcctttgc
     4681 catcagggtg gcaacatcca cagcaggaac ggtccggggg agcgaaaaaa ccggtttata
     4741 agagatatct gacagtgtcg gttttactga ggcaagtaaa accggatccg ggaggcaaaa
     4801 gcgtttaaga acataccggg attccgggtt caactcgccg caacgggctg gaatatacct
     4861 gccgggttct gacaggtttt taaattccgc gtacccgatc acgcttgcaa tgctgtcggt
     4921 atctggttgc cggtgaccga aaagatatac t
//
LOCUS       DASRLV010000086        15600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_25734,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000086 DASRLV010000000
VERSION     DASRLV010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 15600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15600
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25734"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(477..980)
                     /locus_tag="VFC43_03420"
     CDS             complement(477..980)
                     /locus_tag="VFC43_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZK30269.1"
                     /translation="MPEQPGIKCEYLLVEAGCNEKVVAHCRAVRDVALEYARSNPLVS
                     AELVEKGAMLHDIGRGSNHTIRHAQLGADLLRARGYSEAIARIVECHTGAGLTADECT
                     LLRLSPRDCMPQSTEEKIVTHADNLIDGRKRVSIEETLITAIHLPRRIRKRMYRLSQD
                     VELLCPL"
     gene            complement(973..1494)
                     /locus_tag="VFC43_03425"
     CDS             complement(973..1494)
                     /locus_tag="VFC43_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HZK30270.1"
                     /translation="MDPIDDLLNDPAIRAYLHRLVKDEGLNLIERFPREGEYSDEDLA
                     ARTGINLNSVRHTLYTLYEKRLAEYHRIKNNDTGWLTYLWQLRTDHIYSAIREDMELI
                     LEKLQRREKYEEENDFFICKDCQVLFTFPQAMSCDFNCPGCQKAVTHFDNDMLLRSLK
                     HRIGDIKKSLGHA"
     gene            complement(1494..2570)
                     /locus_tag="VFC43_03430"
     CDS             complement(1494..2570)
                     /locus_tag="VFC43_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZK30271.1"
                     /translation="MKGRVLVVGFSTRHVVQSAARAGYEVCAVDHFCDQDLCWITKDR
                     ERFADLADLPAAIERICRRHTFDFLVVTSGAEDLSPPVSLCGTPKEKIGRFLDKLETQ
                     YFFEQLNVPVPKLLPDGKYPAMVKPRRGAGGWRNSIVCDKKGLTEWEDLYPATPFIRQ
                     EIIEGVPASVCCITDGSHARAIAVNEQFLRGNEGSAFGFSGSVTPLDHPLSPVMIMLA
                     EQIAASSGCSGTIGIDFVLGKDSPYAIEVNPRFQGTVDTVEMACACNLFRYHVDACRG
                     ILPPVAPTPNQVAVRSILFADRNITIKCDLKHLASRIADIPWPGTFFEEGQALVSVFG
                     WGKTREDAFSLLDKHISTVRQYIR"
     gene            complement(2567..3097)
                     /locus_tag="VFC43_03435"
     CDS             complement(2567..3097)
                     /locus_tag="VFC43_03435"
                     /EC_number="2.1.1.206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(56)-2'-O)-methyltransferase"
                     /protein_id="HZK30272.1"
                     /translation="MPENGVMILRIGHRPERDQRVTTHVGLTARALGAKGMYLAAADK
                     GVVQSIDDVVSRWGGNFFCQDTIKWRSFIRDWKDQGGIVVHLTMYGLNLPDVIDDIRP
                     CEKVLVIVGAEKVPGEIYGLADFNVAVTGQPHSEISSLALFLDHFFSRTELRNEFPGA
                     RIRIIPSKAGKQTEEM"
     gene            complement(3099..3374)
                     /locus_tag="VFC43_03440"
     CDS             complement(3099..3374)
                     /locus_tag="VFC43_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZK30273.1"
                     /translation="MKEEDADWLIYHLLVKEPSISTEEGLVTASGLDPSAVDASLKRL
                     ERYLLIEQVEGTVRVLSFGEALIRCQIKYSQDLPYTMENGVIKQRKA"
     gene            3478..3891
                     /locus_tag="VFC43_03445"
     CDS             3478..3891
                     /locus_tag="VFC43_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30274.1"
                     /translation="MNPDGSTVSYAELVSIGDHLFEKCEEDVACLASRIDALDTDVRN
                     ELLVSDILNSFQVFYYYFRIFPDELIGERLELEPASSLIRGVKIDEIELLELLFLVKN
                     HEPLIIVSDGEKAITTFKGKTAFADGLKYIENPPY"
     gene            complement(4006..4719)
                     /locus_tag="VFC43_03450"
     CDS             complement(4006..4719)
                     /locus_tag="VFC43_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HZK30275.1"
                     /translation="MIRAPFVFRETFATILADEPEHIEAAKTGMLAARQALEAYIAYD
                     PFFLSTFDPYEPDSDDELILRMTDATKKAGVGPMAAVAGAIAWAGVEAMLNAGAVFGV
                     IDNGGDIALVSDRPVRVGVHAGPAALSNRVAFVVPPQEKILGICTSSATVGPSISFGI
                     ADAVTIFSHDVALADAWATSVCNLIRPDDQAVFYRVNADEVLGILAIMGDLIVKWGTL
                     PPLVPAVVDEHLISAGDRL"
     gene            complement(4716..5102)
                     /locus_tag="VFC43_03455"
     CDS             complement(4716..5102)
                     /locus_tag="VFC43_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZK30276.1"
                     /translation="MKLLVNFSRKKGSKPIIAQVVRDTGVLINVERAVIDSSEGEALI
                     DVPDDQCRIVSDSMISLGANVRILENGISVNESECVDCGACISICPREVFSMDPEWKL
                     HLAEEKCVLCGKCIEACPHQALTLPL"
     gene            complement(5099..6607)
                     /locus_tag="VFC43_03460"
     CDS             complement(5099..6607)
                     /locus_tag="VFC43_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HZK30277.1"
                     /translation="MHKSIDQINQRIRDGTVHVVTAEEMPSIVAELGEEGALKEVDVV
                     TTGTFGAMCSSGAFFNFGHAEPPIRMERIWLNNVEAYGGLAAVDTYIGATQPSDTLEE
                     KYGGAHVIEDLVAGKSVELRASSRGTDCYPRRTITTDLLLENLNQAIMCNPRNAYQRY
                     NAATNSTDRILHTYMGVLLPNFSNVSFSGAGTLNPISNDPQLRLIGSGVPIFLCGAQG
                     MVVGEGTQHSPAGGYGTLMVTGNLKEMSQEYMRAATMTGYGITLYVGLGVPLPVLDLE
                     VVRATAVRDEDISVDIMDYGVPSRNRPSIRKVTYAELRSGSVEINGEEVRTSSLSSYR
                     KARQVSKELKAWIERGSMQLALPTRPIDAKKSAKPMHQISKGPRVLDIMDRNVVSIGE
                     NEEITTAAKKLLKGETNHLPVINGDNVLVGIVTTFDVSKAVVNPGKAHFVKDIMKRKV
                     ITTTPDEAVDIAVQKLEQHNISALPVVDGGHHVIAILTAMDLGKLFGGRWLK"
     gene            6699..7406
                     /locus_tag="VFC43_03465"
     CDS             6699..7406
                     /locus_tag="VFC43_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HZK30278.1"
                     /translation="MHVRTFIEEMERLAPSVLAEDFDAQKIGLVVEGRQEISRICCAL
                     DATPHVINKAVLASADMLVVHHTPLWTPVTTVTGRVADLMRALLSADMNLYVMHTNFD
                     RAPEGVNDALAELLSLRDITPMTLGCIGTSMLSAAELSKRLGGHIRLWGEVKKLTRLA
                     IVAGSGFDSALMAEAKEKGADVFLSSEMKHSVARMAPLPCIEATHYALEAPAMQRLAT
                     RRGWQYFDDPPFLKVIP"
     gene            7403..7732
                     /locus_tag="VFC43_03470"
     CDS             7403..7732
                     /locus_tag="VFC43_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HZK30279.1"
                     /translation="MSDLWQRLSEKKVLDDSIRLDIEDTLGSRGKKALAALDTGKIKK
                     YLDFFVVQGRTAEYIVDEDFCTCGDFLYRKKICWHLLAVRIAQETGMYKKIESWYQDM
                     WKNKEIP"
     gene            7781..10522
                     /gene="alaS"
                     /locus_tag="VFC43_03475"
     CDS             7781..10522
                     /gene="alaS"
                     /locus_tag="VFC43_03475"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HZK30280.1"
                     /translation="MLEEEYQLEYFKTQGLTRKICKSCGSAFWTRDHLREICGDAPCE
                     PYNFIGNPIFKTHTLDSMREAYLSFFEKHGHTRIERYPVVARWRDDIYLTIASIADFQ
                     PFVTGGIVPPPANPLTISQPCIRLNDLDSVGRSGRHLTTFEMMAHHAFNTPAEEIYWK
                     DRTVELCDQFIASIGGDITKVTYKEHPWIGGGNAGPSVEVLIGGLEIATLVFMSLGRQ
                     KTSETGYELNGEMYYPMKMRIVDTGYGLERLVWASKGSPTIYDAVFPEMVSRVMSAAG
                     LSHMLDNKDYTKILALNAKFAGLMDISGTNLFQLRKKVAAAIDVSPEKLDKMITPIEK
                     VYAIVDHTRCLAYMLGDCIVPSNVREGYLARLVIRRTLRLMNELNIKDPLADLVEHQT
                     KIIGLKKFEQDISVVREIVDRETDKYASTLERGSRIVQKIAKSYKAKSQRVPFAEIIT
                     LYDSHGIQPEMVKDIAAKEGAVVDLPDNFYSMVADMHSESKKEVAEDTTSKYAGRVDG
                     LPMTKKLYYEQPSDIEFEAVVLDFFDGYAVTDQTLFYPDGGGQPADTGTLVSSDSMVR
                     VDGVIKIGEVILHHVTGGVLHRGERVKGMVDEERRWSLMRHHTATHILLHAAKDVLGA
                     HIHQAGAQKGSESSRIDIRHFKHITPEELIKIETEANRMIMTSQPVEVSIEDRTKAEQ
                     KYGFSLYQGGVPPGREIRVIKVAGDIEACAGTHCRNTGEVGVIKIIRVEHIQDGIERI
                     EFSAGLAAIFYMQHLEHIVTSSSEVLSVQPDNLPSTVSRFFTEWKDQKKEIDRMSARL
                     VDLELQTLVAESVSGIAVVIKRIDLPAKELTALALSISEKGGIALLAGAGDTARVVLT
                     SANPQVNAGEIITQVCSILGGKGGGKPTMAQGGGPNVNQIDLALKVGRERIIAALHG"
     gene            10515..11249
                     /locus_tag="VFC43_03480"
     CDS             10515..11249
                     /locus_tag="VFC43_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZK30281.1"
                     /translation="MADNVVILEPGDERAQKISKAMASQTASDILQLLADNKKSLTEI
                     TDRLSIPLTTAKYHIENLLEAGLITVAETKYSVKGREIKIYAVTNQLLIVAPRQSNVR
                     SLLLKYATLFGVVAFGSLAISLLSPILGQGIMTDSSLNGPPRMETFSYAATPAKTVSE
                     GILPNSTLTPDVVINGSKGISEIAVKNITSVPSPIANPVPVPPGTGASATAALPDIAL
                     AFFLGGMIVIIVLVMYEIYLWKKMKS"
     gene            complement(11696..12523)
                     /locus_tag="VFC43_03485"
     CDS             complement(11696..12523)
                     /locus_tag="VFC43_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30282.1"
                     /translation="MKKILFIALFLMCSVGSASAYQLYLSCPETVQVGLPLKCSIDSN
                     FPAGTTFDVVVYQSGYTATSLRRQSVTIQENHATQYQLIDTKGLPGGQYKVEIQFIGP
                     DEGKLSTDSVILQLPRLLDRSNEITITSPMTQTSDEPLRIEGSVTKVGNEGVQIEVTG
                     PDGVIFGPQWIGTKEYIQSGAGEFTQQVSVSKPGDYEVSFRDSKGYIGVKTFKVISPA
                     TPVPTTVPTTAPVTTIPSTTVTTVLPTTTQAPLSFLSIITALSLVGMVSVILMKKHG"
     gene            complement(12612..13214)
                     /gene="hisH"
                     /locus_tag="VFC43_03490"
     CDS             complement(12612..13214)
                     /gene="hisH"
                     /locus_tag="VFC43_03490"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HZK30283.1"
                     /translation="MKIAIIDYGLGNLRSVIRGLEKAGANAVITSKEEEIAAADGLVL
                     PGVGAFREGMDQLGSLKDTVKEATRDVPLLGICLGMQMLMETSEEHGIHQGLGLVPGT
                     VKRFPRGSGQKVPHMGWNSLTIEQPDNPLFAGFGGDEYVYFVHSFFADTAPEYTLTST
                     KYICSFSSSVINKNTFGVQFHPEKSGAIGLRLLNNFIGLM"
     gene            complement(13195..14607)
                     /locus_tag="VFC43_03495"
     CDS             complement(13195..14607)
                     /locus_tag="VFC43_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HZK30284.1"
                     /translation="MRPSYLGKILLRWCDTCHAPVLSHLCSCGAKTREVPVTPPGDAR
                     PAFPEDVTLINRIFKDHFGANLIPKGNLALLNKVPDQDRMEEIIVGGGIAGIIRYLPQ
                     QRRWEPIPRPEACSLFSPKKRFIIVDDDAVPFIRDQGMSVLACGLVTIEDSVHSGDEI
                     FILAQDGSCIGVGRAKVDAATARKMEKGSLARTRRNIASEIVPGEACWDAAVTANSAV
                     LHNTESESIQFVRMVREQNPHLVANVSYSGGKDSLATLLVVIKAIGKVPMLFADTGFE
                     FPETYANVDNASRHYGLDVIRTDGNTRFWENFGRQGPPAVNARWCCRVCKLTPVANLI
                     REQWGECLSFIGQRRYESAARAQSDRVWRNKNVRGQLSAAPIHNWTALHVWLYLMREK
                     APYNILYEHQLDRIGCFMCPSSDMALIHMIEAEYPHLWESWRTQLEQWQQVQGLSADW
                     VTAGKWRIKEGCEADEDSNY"
     gene            14765..15025
                     /locus_tag="VFC43_03500"
     CDS             14765..15025
                     /locus_tag="VFC43_03500"
                     /inference="COORDINATES: protein motif:HMM:NF015993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HZK30285.1"
                     /translation="MIDTWLFAVLCLIFLSICAVGRAIPGPTHDDQLIGVNTAITIAA
                     GAALALSISWGNLFLLDVAIAIITLCYAGTIAYSRALKGETL"
     gene            15022..15378
                     /locus_tag="VFC43_03505"
     CDS             15022..15378
                     /locus_tag="VFC43_03505"
                     /inference="COORDINATES: protein motif:HMM:NF015302.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HZK30286.1"
                     /translation="MSDLFLDSLITILLVISVGFGSIGVIGLLLFPDIRIRMFTATRA
                     TIISFGTITLAVILYALYAFQSDGGDQYITLVFHSVIFLCILIIANIVQYKTILYRTR
                     TMTSCQKLSDPPTLEK"
BASE COUNT         4074 a         3873 c         3627 g         4026 t
ORIGIN      
        1 gtggtccatg ttcttgcccg tcatgatgtc tccatacggc agatctttgt cactgacccg
       61 gagttttctg aagagccccg tctcgttatt attattgaag aaaccatgcc tgtcggagtc
      121 attgaacaaa tccgtgcctt accccaggtt aaacaggtca tcatctagga aaactaatgt
      181 aaccccaaac ccccttaaat ctccctgcct tttttaggga acggagcagc aaaagtatta
      241 tcagatcagc acgggattta tcggtatgcc tcagcggact ctgactgcgt ggctttgccc
      301 gcctgcctgc agttcctctg caaaaaagaa aaagataaaa ttgcgtagag agaattttat
      361 gacaggggat aaaataagca aaatttattc ccgtacaaaa actctccaga aataaaaata
      421 tttaaatctg aatgcttttt ccggtgtatc aattcttcag aaacttttga tccagtttac
      481 agggggcaaa gaagttcgac gtcctgtgaa agacgataca tccgtttcct tatcctcctt
      541 ggcagatgaa tggcagttat cagggtttct tctatactca ccctctttct accgtcaatg
      601 agattatccg catgagtaac tatcttctct tctgtactct gcggcatgca gtcacgggga
      661 gataacctga ggagtgtaca ctcgtcagcg gtaagtcctg ctccggtgtg acattcaaca
      721 atacgggcaa tcgcctcact gtatccccgg gcacgtagca gatccgctcc cagctgagcg
      781 tgccggatag tatggttact ccctctcccg atatcgtgga gcatcgctcc tttctcaaca
      841 agctctgctg aaacaagggg attggaccgg gcatattcca gtgcgacatc ccttacggcg
      901 cggcagtgag ccacaacttt ttcattgcat cctgcttcca caagcaggta ttcgcatttt
      961 ataccgggtt gttcaggcat gacccagtga ctttttaata tcaccaatgc ggtgtttgag
     1021 tgagcgcagg agcatatcat tgtcaaaatg cgttaccgct ttctgacaac ccgggcagtt
     1081 gaaatcacag ctcattgcct gaggaaaggt gaagagtacc tgacaatcct tgcagataaa
     1141 gaagtcattt tcttcttcat atttctcacg tcgctggagt ttttccagta taagttccat
     1201 gtcttcccgg atcgcactat agatgtgatc ggtacggagc tgccagagat atgtcagcca
     1261 gccggtatca ttgtttttga tccggtgata ctcggcaagc cttttttcat agagggtata
     1321 gagtgtatgt cgtaccgaat tcaggttgat tccggtgcgt gccgcaaggt cttcatcgct
     1381 atattcccct tcacgtggaa accgttcgat aagattgagt ccctcatctt ttaccagtcg
     1441 atgaaggtag gctcgtatcg cgggatcgtt cagcagatcg tctatgggat ccatcaccgt
     1501 atgtattgtc ggacggtgct aatatgttta tccagcaaag agaaggcatc ctcccgggtc
     1561 tttccccatc caaagacact taccagagcc tgaccctctt caaaaaaagt acccggccag
     1621 ggaatgtcag caatacggga tgcgaggtgt ttgagatcgc acttaatggt gatattcctg
     1681 tcagcaaaga gaatgcttct gacggctacc tggtttggtg tgggagctac cggaggaaga
     1741 atccccctgc aggcatcgac atgataacgg aacaggttgc aggcgcaggc catctcaaca
     1801 gtatccacgg ttccctggaa acggggattc acttcgatgg catagggaga gtcttttcca
     1861 agaacaaaat cgatcccgat tgtaccgctg cacccgcttg atgcagcgat ctgctcggcc
     1921 agcataatca tgaccggact gagtggatga tccaggggtg tgaccgaacc gctgaacccg
     1981 aatgctgacc cttcattacc ccgtaaaaac tgctcattta ccgctattgc ccgtgcatgt
     2041 gaaccgtcag tgatacaaca aacactcgcc gggacgcctt caataatttc ctgccggata
     2101 aatggcgtag ccggatacag gtcctcccac tctgtgagcc cttttttgtc acacacaatg
     2161 gaattccgcc atcctcctgc acccctcctt ggtttaacca tcgcggggta cttcccgtca
     2221 gggagaagct tggggaccgg aacgttcagt tgttcaaaaa aatactgtgt ttccagttta
     2281 tccaaaaaac gcccgatttt ttctttgggt gtcccacaaa ggcttacggg gggggacagg
     2341 tcttctgcac cagaagttac gacaaggaag tcaaatgtat gacgcctgca tatcctttct
     2401 atcgcagcag gaagatcagc gagatcagca aacctttccc ggtcttttgt aatccagcaa
     2461 agatcctggt cacagaaatg atcgaccgca cacacctcgt atccggcacg ggcggcagac
     2521 tggactacat gccttgttga aaatccgaca acaagaaccc tgcccttcac atctcctccg
     2581 tctgttttcc cgcttttgat gggatgatac gtatccgtgc tccgggaaat tcatttctga
     2641 gttccgttct ggaaaaaaaa tgatcgagaa aaagagcaag acttgatatt tcagaatgtg
     2701 gctgacccgt aacagcaacg ttaaaatccg caagtccata aatctcgcca ggtactttct
     2761 cggctccaac gataacaagg accttctcac aaggtctgat atcatcaatc acatcgggca
     2821 ggttcaggcc gtacatggta agatgcacaa cgatgccccc ctgatctttc cagtcccgga
     2881 taaaagagcg ccattttatg gtatcctggc agaaaaaatt tccaccccaa cggctgacca
     2941 catcgtctat gctctgaaca acccccttat cagcagcagc cagatacata ccttttgcac
     3001 cgagtgcccg ggcagtcaga ccgacatggg tcgttacacg ctggtcgcgc tctgggcgat
     3061 gtccaatcct gagaatcatt actccatttt ctggcatact acgctttccg ctgtttgatc
     3121 acaccatttt ccatggtata ggggagatcc tgtgagtatt ttatctggca gcggatgagc
     3181 gcttcgccaa aactgagtac acgcacggtt ccctccacct gttcaatcag aagatatcgt
     3241 tcaagacgtt tcagagatgc atccacagca ctgggatcaa gtccgctcgc ggtaacaaga
     3301 ccttcttctg tcgaaattga aggctccttc acaagaaggt gatatatcaa ccagtccgca
     3361 tcctcttctt tcacctttat tcattcctca tggtggtgaa taagcattgt gtcccgcgat
     3421 ttaagaaatg cactttcatt gccatttttt ttaagcctca atgacaaatg acttgcaatg
     3481 aatcctgatg gatcaacggt ttcttatgct gagctggttt caattggaga tcacctcttt
     3541 gaaaagtgcg aggaggatgt tgcctgcctt gcttcaagga tcgatgcact cgatacggat
     3601 gttcgcaacg aattgctggt ctccgatata ctgaattcat ttcaggtatt ctattattat
     3661 tttcggatat tccccgacga actgattggc gagcggcttg aacttgaacc agcatcatcc
     3721 cttattcggg gggttaagat cgatgagatc gaactccttg aattactttt tttagtaaaa
     3781 aatcacgagc cgctcatcat tgtttctgat ggagaaaaag caattaccac ctttaaggga
     3841 aaaacagcgt tcgctgatgg actaaagtat attgagaatc ccccttactg acaaaaatcc
     3901 tcacagtagg ttggcagtgt ccgaatacta ggactaaaac acaacaagtg cacacaaatt
     3961 caacattttt ttaagcacat cttcgaagag aacgtgcttc ttgatctata aacgatcccc
     4021 cgcacttatc agatgttcat cgaccactgc aggtaccaag ggaggtagag ttccccattt
     4081 tacgataagg tctcccataa tggctaaaat gcccaggact tcatcagcat taacgcggta
     4141 aaaaacggcc tggtcatccg gacggataag gttacagact gaagttgccc atgcgtctgc
     4201 aagcgcaaca tcgtgtgaga agatggtaac tgcatcggct atcccgaatg atattgaagg
     4261 ccctacagtt gctgatgacg tgcatatgcc aagtattttt tcctgagggg gcactacgaa
     4321 tgcaacccgg tttgaaagtg ccgccggccc ggcatggact ccgacacgga ccgggcggtc
     4381 acttacgagg gctatatcac ccccgttatc aataactcca aatacggcac ctgcattcag
     4441 catcgcctca acacctgccc atgcaattgc acctgcaact gcagccatcg gaccaacacc
     4501 ggcttttttg gttgcatcag tcattctgag aatcagctca tcgtcagaat ccggctcata
     4561 gggatcaaaa gttgagagaa aaaaaggatc atatgcgata tacgcttcca atgcctgccg
     4621 tgccgcaagc atgccggttt ttgccgcttc gatatgctcc ggttcatccg caaggatcgt
     4681 tgcaaaggtc tcgcggaaca caaagggagc acggatcata gcggaagtgt gagcgcctgg
     4741 tgcggacatg cttcaatgca tttcccgcag agtacgcatt tttcttcagc cagatgcagt
     4801 ttccattcag ggtccatgga aaacacttca cgtggacaga tgctgataca tgccccacaa
     4861 tccacgcact cactctcatt cacactgatc ccgttttcaa ggatccgcac attcgcgccc
     4921 aggctgatca tggaatcact gacaatccgg cactgatcgt ccggtacatc gatgagcgct
     4981 tcaccctctg atgagtcaat cactgcccgc tcaacattga tcaggactcc ggtatcccgg
     5041 actacctgtg cgataatggg cttactgcct ttcttgcgcg agaaattcac cagcagtttc
     5101 atttcagcca cctccccccg aacagcttcc cgagatccat tgcggtcagt atggcaataa
     5161 catggtgacc gccatcaaca acaggaaggg cactgatatt atgctgctca agtttctgta
     5221 cggcaatgtc caccgcttca tccggggtgg tcgtgatcac tttccttttc atgatatctt
     5281 taacgaaatg tgcctttccg ggatttacga ccgctttgga gacatcaaac gtggttacga
     5341 taccgaccag gacattatct ccgtttatca ccggaagatg attcgtctca cccttgagaa
     5401 gtttttttgc tgcggtcgtg atctcttcat tttcaccgat actcacaaca ttcctgtcca
     5461 tgatatccag cacacgcggc cctttggaga tctggtgcat gggttttgct gatttttttg
     5521 catcaattgg ccgggtagga agcgcaagct gcatacttcc ccgttctatc caggctttca
     5581 gttcctttga gacctgacgg gctttccggt aactggaaag cgaagatgtg cgcacttctt
     5641 ctccatttat ttcaactgag ccgctccgca attcggcata ggtcactttc cttatggagg
     5701 gacgattccg gctgggtact ccgtagtcca tgatatcaac agatatatcc tcatcacgca
     5761 ccgcagtagc tcgcactacc tcaagatcca gcaccggcaa cgggacacca agccccacat
     5821 agagcgtgat accataaccg gtcattgttg ccgccctcat gtactcctgg gacatctctt
     5881 ttaaattacc cgtcaccatc agcgttccat atccaccagc gggggaatgc tgggtcccct
     5941 caccgactac cattccctgc gctccgcata aaaagatagg cacgccactt ccaatcaggc
     6001 gaagttgtgg atcattggaa attgggttta atgtaccggc tcctgaaaag gatacgttgc
     6061 tgaaattcgg caggaggacg cccatatagg tatgcaggat gcgatctgta gaattggttg
     6121 cagcattata tcgctggtac gcattgcgtg gattgcacat aattgcctgg ttgagatttt
     6181 ccaggaggag atccgttgta atcgtacgcc gcggatagca gtctgtcccc cgtgaacttg
     6241 cacgaagttc tacagattta cccgctacaa gatcttcaat gacatgagcc ccgccatatt
     6301 tttcttcaag tgtatcggac ggttgtgtcg ctccgatgta ggtatcaact gccgcaagcc
     6361 ccccgtatgc ttcaacatta ttcagccaga tccgttccat cctgataggt ggctcagcat
     6421 gcccgaaatt gaaaaacgca cctgatgagc acatagcgcc gaaggttccg gtagtgacta
     6481 cgtcaacttc ctttaaggcg ccctcttcac ccagttcggc aacaattgac ggcatttcct
     6541 cagccgtgac tacgtgcacc gtgccgtccc ggatccgctg gttgatctga tcaatggact
     6601 tgtgcatacc cagaaatgcg ttttaatgca taataaaatt aattgttatt actcatagta
     6661 atatatgaca ttttaaacct aaacgacgat acttgtctat gcatgtgcgt accttcattg
     6721 aagagatgga acgtcttgct ccaagtgtcc tggctgagga tttcgatgca caaaaaattg
     6781 gcctcgttgt cgaaggaagg caggaaattt ccaggatttg ctgcgcactg gatgcaacac
     6841 cccatgtaat aaataaagca gttctggcgt cggctgatat gctggtagtg catcatacac
     6901 ccctatggac accagtgaca acggtgacgg ggcgggtcgc agatctgatg cgcgccctgc
     6961 tctccgctga tatgaacctc tatgttatgc atactaactt tgatcgagca cccgaaggcg
     7021 taaatgatgc actcgcagag ctcctgtcac ttcgggatat tacccccatg acgctcggct
     7081 gtattggtac ttcaatgctt tcagcggctg aactctcaaa acggctcggg gggcatatcc
     7141 ggctctgggg agaagtaaaa aaattaacca ggcttgcaat tgttgccggg agcggttttg
     7201 attcggcgct catggcggaa gcaaaagaaa aaggggcaga tgtattcctt tcttctgaaa
     7261 tgaagcattc agtagcacgc atggctccgt taccctgtat tgaggcaaca cattatgcac
     7321 tcgaagctcc ggcaatgcag cgcctggcta cccgtagggg ctggcaatat tttgatgacc
     7381 cgccatttct gaaagttatc ccatgagcga tctctggcag cgtctttctg aaaaaaaagt
     7441 tctcgatgat tctattcgtc tggatattga ggatactctc ggttcacgag ggaaaaaagc
     7501 ccttgcagca ttagacaccg gtaagatcaa gaagtacctg gatttttttg tcgtgcaagg
     7561 caggactgct gaatatatag tggatgaaga tttctgtacc tgcggggatt tcctgtaccg
     7621 taaaaagatc tgctggcacc tgctggcagt caggattgca caggaaaccg ggatgtataa
     7681 aaaaatcgaa agctggtacc aggatatgtg gaaaaataag gaaataccat agaataatta
     7741 ccgttcataa tcaattgtac tgattattac gtgatccttc atgctcgaag aagaatacca
     7801 gctggaatat tttaagaccc agggacttac tcgcaagatc tgtaaaagtt gcggttcagc
     7861 cttctggaca cgtgaccatt tgagggagat ttgtggcgat gctccctgtg aaccctataa
     7921 ttttatagga aatccgattt ttaaaaccca cacactcgac agcatgcgtg aagcctatct
     7981 ctctttcttt gaaaaacatg ggcatacccg tatagagcgt tacccggtag ttgcacgatg
     8041 gcgcgatgat atttatctta ctatcgcgtc catcgctgat ttccagcctt tcgtgaccgg
     8101 gggaatcgta ccccctcctg caaatcctct caccatttca cagccatgta tccggcttaa
     8161 cgatctcgat tccgttggca ggtcaggaag gcacctcaca acctttgaga tgatggcgca
     8221 ccatgcattc aacacacccg cagaagaaat ctactggaaa gaccggaccg tcgagctctg
     8281 cgaccagttc atcgcatcta tcggggggga tatcaccaaa gttacctata aagagcaccc
     8341 ctggatcgga ggaggtaatg caggaccgag cgttgaagtg cttatcggcg ggcttgagat
     8401 cgctacactg gtcttcatga gcctcggccg gcaaaaaaca tcagaaacag gatacgaact
     8461 gaatggagag atgtactatc cgatgaaaat gcggattgtc gataccgggt atgggcttga
     8521 acgcctagtc tgggcatcca aaggttctcc caccatttat gatgcagttt ttcctgaaat
     8581 ggtcagccgg gttatgagtg ctgccggtct ttcgcatatg cttgataaca aggactatac
     8641 caagatcctc gctctcaatg cgaagtttgc aggtcttatg gacatctccg gcaccaatct
     8701 cttccagctg cgcaagaaag tcgcagcggc cattgatgta tctccggaaa aactcgataa
     8761 aatgatcaca cccatagaga aggtgtatgc gattgtcgat catacgcgat gccttgccta
     8821 tatgctcggg gattgcatag tcccatccaa tgtccgtgag ggttatctcg cacgccttgt
     8881 gatccggaga acactgaggt tgatgaatga gctcaatatc aaagatcctc tcgcagatct
     8941 ggttgaacat cagacaaaga ttatcgggct gaaaaaattc gaacaggata tttcagtagt
     9001 ccgcgagatt gtcgaccggg agactgataa gtacgcatct accctcgaac gtggttcacg
     9061 gattgtgcag aaaatcgcaa agtcgtacaa ggcaaagagc cagcgcgtcc cgtttgcgga
     9121 aatcattaca ctttacgact cacatggtat ccagccggag atggtaaagg atatcgcagc
     9181 aaaagaaggt gcggttgtcg acctccctga taatttctac tcaatggtgg cagacatgca
     9241 ctccgagtca aaaaaagagg ttgctgaaga taccacttcg aaatatgccg gacgggtgga
     9301 tggtcttccc atgactaaaa aactctacta cgagcagcct tccgatatcg agtttgaagc
     9361 tgtcgtgctt gacttttttg acggctatgc agttaccgac cagacactat tctacccgga
     9421 tggcggggga caaccggcag acaccgggac actggtgagt tccgacagca tggtgagagt
     9481 cgatggagta ataaaaatag gggaagtgat actccatcat gtcaccgggg gagtacttca
     9541 ccgcggagag cgggtaaaag gaatggtgga tgaggaacgc cggtggtcgc tgatgcgcca
     9601 ccacacggca acccatatcc tcctgcatgc tgcaaaagat gtgctggggg cgcatattca
     9661 ccaggcagga gcccagaaag gcagcgaaag ttcacggatt gatatccggc acttcaaaca
     9721 catcactcct gaagaactca ttaagattga aacagaagca aaccggatga ttatgaccag
     9781 ccagccggtt gaagtttcga ttgaagaccg cacaaaagcc gaacaaaaat atggattttc
     9841 cctttaccag ggaggagttc cgccgggcag ggaaatccgt gtgatcaagg tcgcaggaga
     9901 tatcgaagcg tgtgccggca cgcactgcag gaataccggt gaagttggcg taatcaagat
     9961 catccgggtt gaacatatcc aggacggaat tgaacgtatc gaattttctg ccggactggc
    10021 tgcgattttc tatatgcagc atctcgaaca tattgtgact tcatcttcag aggtgctttc
    10081 tgtacaacca gacaaccttc cttcgactgt ttcacgtttt ttcaccgagt ggaaggatca
    10141 gaagaaagag atcgatcgaa tgagtgcaag acttgttgac cttgagctgc agacactggt
    10201 agcggaatct gtcagcggta tcgctgttgt aattaaacgc atcgatctcc cagcaaaaga
    10261 actgactgca cttgcactat ccatttcaga aaaaggaggt atcgctctcc ttgccggagc
    10321 gggtgatacg gcaagggttg tgctgacttc tgcaaacccg caggtaaatg ccggagagat
    10381 catcacgcag gtctgcagca ttctcggagg aaagggagga ggcaaaccaa ccatggcaca
    10441 aggtggcgga cccaatgtta accagattga tcttgctctg aaagtcggac gcgaaaggat
    10501 aattgcggcg ctgcatggct gacaacgttg taatccttga gcccggcgat gaacgggccc
    10561 agaaaatttc caaggcaatg gcaagccaga ctgccagtga tatcctccag ctgcttgccg
    10621 ataataaaaa gagcctgacc gagattaccg acaggctttc cattccgctg acaaccgcaa
    10681 aataccatat cgaaaatctt ctcgaagcgg gcctgatcac ggttgcagag acaaagtaca
    10741 gcgtcaaagg gcgtgaaatc aagatctacg ctgttacaaa ccagctcctg atcgttgctc
    10801 cccgacaatc caatgtgcgt tcgctcctcc tcaaatatgc aacccttttt ggggtagtcg
    10861 ctttcggatc gcttgccatt tcactgcttt ccccgatact tggtcagggt atcatgacag
    10921 acagcagtct gaacgggcca ccacgcatgg aaacattttc ctatgcagca acgcctgcca
    10981 aaacagtgtc tgaagggatt ttgccaaatt ctaccttgac tccggacgta gtaataaacg
    11041 gctccaaagg gatttctgag attgcggtaa aaaacataac atccgtacca tctccgatag
    11101 caaatcccgt accagttccc ccaggaaccg gagcctctgc cacagccgct ttaccggata
    11161 tcgcacttgc gtttttcctt ggcgggatga ttgtcatcat tgtcctcgtt atgtatgaaa
    11221 tatatctctg gaaaaaaatg aaatcgtgat catttcatgg cagatccctt tttttgataa
    11281 ttaattaaat atttcctccg ttgaaatcat tctgaaaatt ctgacgctct tgggggatac
    11341 gcccccgcca ctgtggcatt cccattgcga tgacgatgga ttacctgctt ccaaactcgg
    11401 gttatcgaat tgcgccccat cctccaacgg agaattgggg ggtgaaagag gtggaattat
    11461 tttcacagga aaaaaagttt tttacacagc aaaagaatcg tggtaaatcg catccccaat
    11521 attgctgccc gaatgttcaa aatatatgcc cattcatgtg ttggtctggc ctttttaaaa
    11581 aattccgtta ccaatacgta agggaaactg tgaggaagtg atgaacaaac acgtgagcct
    11641 tggtgctaat gaacagttac catcagatgc aatattattt aaaaaagtga aaggcttatc
    11701 catgcttttt catcagaatt accgatacca ttcctacaag tgagagggcg gtaataattg
    11761 acagaaaaga cagaggtgcc tgagttgttg ttggcagaac ggtggttacc gttgttgacg
    11821 gtatggtagt aaccggtgca gttgtcggaa ccgttgtcgg gacaggagtt gccggtgaaa
    11881 taaccttgaa tgtctttacc ccaatatagc ccttggaatc cctgaaagat acctcatagt
    11941 caccgggctt ggagacagat acctgctggg tgaattcgcc cgccccggat tgaatatact
    12001 cttttgtacc tatccactgg ggcccgaaaa taacaccatc aggacctgta acttcaatct
    12061 gcacaccttc atttcccact tttgtaaccg atccctcaat tctgagcggc tcgtcagaag
    12121 tctgggtcat cggggatgtg atggtaattt cgttcgatcg gtcaagaagt ctgggcaact
    12181 gcaggataac cgagtcagtt gagagcttgc cctcatcagg cccgatgaac tggatctcca
    12241 ctttgtattg accaccgggt aatccttttg tatcaattaa ctggtactgg gtagcatgat
    12301 tttcctggat agtaacggac tggcgtctga gggaagtagc agtatatcct gactggtaaa
    12361 caacaacatc gaaggtcgtc cctgcaggga agttgctgtc tatcgaacat tttaacggga
    12421 gaccaacctg aaccgtttcc ggacagctga ggtagagctg gtacgcggag gcacttccga
    12481 cactacacat caggaataac gcgatgaaca ggatcttttt catatctaca gcatcgaatg
    12541 gccgaaatta aaaggtttcc cgttagtaaa gaaagagaaa gaaaatgctt gttgaggtta
    12601 ctggataaag actacatcaa tccaataaaa ttattcagca gcctcagacc gatcgcgcca
    12661 cttttttccg gatgaaactg tacaccaaag gtgtttttgt ttatcactga tgatgaaaaa
    12721 gaacagatat actttgttga tgtaagggta tattccggag cggtatctgc aaaaaaagaa
    12781 tggacaaaat acacatattc atcaccgccg aacccggcaa aaagcgggtt atccggctgt
    12841 tcaatagtta aggaattcca tcccatatgc ggaactttct gtcccgagcc acgcggaaac
    12901 cgttttaccg tgcctggcac aagcccgagg ccctgatgaa taccgtgctc ctcacttgtt
    12961 tccatcagca tctgcattcc aaggcatata cccagcaggg ggacatcccg ggttgcctct
    13021 tttacggtgt cttttaaaga acccagctgg tccattccct cacggaacgc cccgactccg
    13081 gggagaacga gaccgtctgc agcagcaatt tcttcttcct ttgacgtgat aacggcattc
    13141 gctccggctt tttcaagacc gcgtataacg cttcgcaggt ttccaagacc gtaatcaata
    13201 attgctatct tcatcagcct cacacccttc cttaatccgc cattttccgg cagttaccca
    13261 gtctgcagaa agcccttgta cctgttgcca ctgttcgagc tgtgtgcgcc agctttccca
    13321 aaggtgggga tattccgcct ctatcatatg tatcaatgcc atatcactcg aagggcacat
    13381 gaaacatccg atccggtcca gttgatgctc atacaggata ttgtaaggag ccttctcccg
    13441 catcaggtag agccagacat ggagagcggt ccagttgtgt atgggagcgg cagagagctg
    13501 accacggaca tttttgtttc tccagacccg gtcactctgt gcccgggcag ctgactcgta
    13561 cctcctttga ccaataaaag aaaggcattc accccactgt tccctgatca ggttcgctac
    13621 cggcgtcaat ttacataccc tgcagcacca gcgggcgttc actgccggag ggccctgtcg
    13681 tccaaaattt tcccagaacc gggtgttccc atcagtacgg ataacgtcca gtccgtaatg
    13741 acgggatgca ttgtcaacat tggcataggt ttcaggaaat tcaaaacctg tgtcggcaaa
    13801 aagcataggt acctttccga tggcttttat cactaccagt aatgttgcaa gactatcctt
    13861 tccaccggaa tatgacacgt tagccacaag atgcgggttt tgctccctaa ccatccgtac
    13921 aaactggatt gattctgatt ctgtattgtg aaggactgcg gagtttgctg ttactgctgc
    13981 atcccagcag gcctcaccag gaacaatttc cgaggcgatg ttccgtcggg ttcgcgcaag
    14041 agatcctttc tccatcttcc gtgctgttgc tgcgtcaact tttgccctgc cgacaccaat
    14101 gcacgaacca tcttgtgcaa ggataaaaat ctcatctccg gaatgtaccg aatcctcaat
    14161 tgttaccagt ccgcaggcaa gcacactcat tccttggtcc ctgatgaaag ggacggcatc
    14221 atcatcaacg ataataaacc gttttttggg agagaataac gaacaggctt cgggacgtgg
    14281 tatgggttcc cagcgccgct gttgaggcaa ataccggata attcccgcga ttccgccccc
    14341 aacaataatc tcttccatcc ggtcctggtc aggtacttta ttcaggagag cgaggtttcc
    14401 ttttggtata agatttgcac cgaaatggtc tttgaagatt cggttgatga gtgtcacatc
    14461 ctccggaaat gcaggacggg catctcccgg aggggtgacg gggacctccc gggtttttgc
    14521 accgcaggaa cagagatgtg aaagaaccgg tgcgtgacag gtgtcacacc agcgtaaaag
    14581 aattttaccc agataggacg gtcgcattgg tattcattcg ctctctgcgg ataaaaaatg
    14641 aaaggagagt aaaaagagca ataaactgat agggtgctgt ttttagtgaa ctattccatg
    14701 atatcaggca ctatccggat cgggaggatt gataaatacc ctcccccaca cactattgaa
    14761 gtacatgata gacacctggc tttttgcagt actctgcctt atatttctct cgatctgcgc
    14821 tgtcgggcgg gctattcctg gtccgaccca tgatgatcag ctgattggcg tgaacacagc
    14881 aatcaccatc gctgccggtg cggcacttgc gcttagtatt tcatggggaa atctgttttt
    14941 gctggatgtg gcgattgcca taataacgct ctgctacgct ggaacgattg catattcacg
    15001 tgccttgaaa ggtgaaactc tatgagtgat ctttttttgg atagcctcat tacaatacta
    15061 cttgtgataa gcgttggatt cgggagtatc ggagtaatcg ggcttttact ttttcctgat
    15121 atccgtatcc gtatgttcac tgcaaccagg gcgacaataa tcagttttgg caccattaca
    15181 cttgcagtca ttctgtatgc actctatgcg ttccagtctg acggcggtga tcaatatatt
    15241 accctggtat ttcattcagt tatttttctc tgtattctta ttatcgccaa tatagtgcaa
    15301 tacaagacga ttctttatcg gacgagaaca atgacttcct gccagaaatt atcagatccg
    15361 cctaccctgg agaaataaaa ttcaagtcat gccgtgcccc aaatcttact gatagcgcat
    15421 acgttattat gattattttt tataagaggt aaatagaata tttacccgat ttttaaaggt
    15481 ttttttatga atcgggagag aattcagaga attgcgcatt tcaatccata ttacttatta
    15541 aaatggcttg tgcagagata ctgccatttt aatgtcaaat aagtgctgat cattatgaat
//
LOCUS       DASRLV010000087         7413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1000482,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000087 DASRLV010000000
VERSION     DASRLV010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 7413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7413
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000482"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VFC43_03510"
     CDS             <1..460
                     /locus_tag="VFC43_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HZK30287.1"
                     /translation="FGNITVAYLSASIFLFGGALNGWDGLIHIIPIAMITFFAMLSRE
                     LVKDAEDVYGDKAGDADTVPIRFGIKKTAYLAFFFTVIAALASVIPYMWWGIWYLAGI
                     LLVDTILLIAVFRGLACDTPECMKASGASQLLKAGMFTSLVVFTLSAVFL"
     gene            541..954
                     /locus_tag="VFC43_03515"
     CDS             541..954
                     /locus_tag="VFC43_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HZK30288.1"
                     /translation="MPTVIQKGDTFFAQKGAYFEGNVKIDGNFIVPARTHFWGRLVVT
                     GTLEMGPGSSVALGVDCWNAIIGSHTKIKGPLVAHGDVTLLDHAVVHSVKAGGKVILR
                     TGVQVGDVTSSDTIIVHGKIKSGKLVGKHMKVLGN"
     gene            complement(919..1620)
                     /locus_tag="VFC43_03520"
     CDS             complement(919..1620)
                     /locus_tag="VFC43_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HZK30289.1"
                     /translation="MDFDFLTWNNLMYLIGEITVLIIVGALITAFVLVIISLYSIKKG
                     RLYFPTLIRSGLVFFEGLMKAFFRLLGLEDREMLTFLIKLHNAMNTAAFERIPVPERA
                     IFMPQCLRSSRCPAHLTPEGLKCRSCGQCTIGQVRFNLEKMGYRIFIVPGSSFIKRMV
                     KKYHPKAIIGVGCLSEVKEGIDMADKMGLVVMGVVTLKEGCVETLVNWADVYNVAALG
                     IDPLSIPEDLHMFSD"
     gene            complement(1649..2692)
                     /gene="mtnA"
                     /locus_tag="VFC43_03525"
     CDS             complement(1649..2692)
                     /gene="mtnA"
                     /locus_tag="VFC43_03525"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HZK30290.1"
                     /translation="MNKAPTLWWDATTESLHYIDQTVLPGRYTVTECHTIGHLAAAIR
                     RLEIRGAPALGVAGAYGIALATRVSWKNELNEFFSEVWKDAEILRTTRPTAVNLGWGI
                     DRVLAKIAPLKDIPSVRRVALEEADAIFREDTANCHAIGNHGAELLPDTCTVLTHCNA
                     GALACSSWGTALGVIRSAVSGGKRVKVMACETRPLLQGARLTAWELARDGIEVTVITD
                     STAAHLMRTGTIDAVIVGADRITHDAVFNKIGTYMHAVCARYHNIPFYVAAPLSTFDT
                     SHEGKEVIIEERGREEVTTMGNRVFVPDAAHVKNYAFDATPMDLVTAIITERGVILPP
                     VSTGSILSLKNTQ"
     gene            complement(2689..4041)
                     /locus_tag="VFC43_03530"
     CDS             complement(2689..4041)
                     /locus_tag="VFC43_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HZK30291.1"
                     /translation="MGDVVVIGAGVAGIQAALDIAGHGIHVHLIEREPSIGGHMAQLD
                     KTFPTNDCSMCILSPKMVDVQRNKNITLYTCAEVEKIEGTVGNFTVTVKKHPRFVDES
                     TCNGCGDCIQICPVEVYNRFDAGIGVRKAIYKPQPQSVPDIVIKDPDHCIECGLCYDA
                     CGPGSVLHEDVPKEILIRAASVVISTGYAVFDAQKKPQFGHLLLSDVITSLELERMIN
                     ASGPTGGKIKRMSNGKTPRSVIFIQCVGSRDMTIKRPYCSCVCCMQAIKNALLIKEKH
                     PETEVIVCYMDIRSYGKGYEEYYERAKANGVKFIRGMPSDVYKDRNGMTMQVENSETS
                     EVLVLHPELVVLSVGMGPAEKTAQIAERFGIPLEDTGFIKPVHDAMDTVATIRPGIYL
                     AGTATAPRDIPDSVASGGSAAMRAYMDAVRIPEMADSIASEEPVALRQHKGAVRTRSE
                     "
     gene            complement(4034..4531)
                     /locus_tag="VFC43_03535"
     CDS             complement(4034..4531)
                     /locus_tag="VFC43_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HZK30292.1"
                     /translation="MRMDVQVSGVPLFAAGNATIIGDVTIGEKVGIWFGAVIRADKDR
                     IVIGDRSNIQDNCVVHTSKGFPVTIGTDVSVGHGAILHGCTIGSRVLIGMGAIIINGA
                     HIGDGSIIGAGAVITEGKVIPPDSVVIGVPGKIVKQTGSDQHQHILNNAASYIELATE
                     YSHHG"
     gene            4562..5020
                     /locus_tag="VFC43_03540"
     CDS             4562..5020
                     /locus_tag="VFC43_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine adenylyltransferase"
                     /protein_id="HZK30293.1"
                     /translation="MKVMVGGTFDPLHDGHKHLLKRSFEVAGPGGHVTIGLTSDNFAS
                     RKNHPVRPFALRQAELERFISFTIPSTHWDVEPLNDRYGSALDADFDALIVSEETLPA
                     AIEINKLRRENGKKKVDIHQISCVLAEDGRWISSTRIYRREIDVHGHLLH"
     gene            complement(5189..7069)
                     /locus_tag="VFC43_03545"
     CDS             complement(5189..7069)
                     /locus_tag="VFC43_03545"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HZK30294.1"
                     /translation="MEFTDRSTGYPTSWKWNFGDGKESSDPNPVHTFWSSGDYTITLT
                     ESNEYGTSDVTRAHLIHVIPALKANFFADPKQGKAPLVVKFSDLSTGNPETWIWNFGD
                     GNNASSPNPVHAYTAPGAYQVDLTVTRGINKDSYSQTIIAGGVPATDFSADTTTIGVN
                     SPVRFTDKTVNSPTTWHWDFGDGATSSEQNPSHTYLLKGIYTVSLIATNTNGQDTKVK
                     SNYITVGVRPGADFMTEIPEYQQGTRVQYVRFIDTSVGNPTSWSWDFGDGQSSGDQNP
                     PLHFYNRDGSYTVSLTVKNIFGQDTKVQTNLITVREGPRVDFKADKTRVSVNQYIHFT
                     DLSTNSPIDWKWDFGDGTAGTGQNPDHVYREPGSYDVTLTASDAYTTISHTKRNFITV
                     VNSPHAEFRADRTKGVTPFTVTFTDLTTGNPTRWKWDFGDGELSSDKNPTHTYTTTGI
                     SSTNKFTVTLSALNSYGEDTLSKVDYITVTQSPIAEFTVDSRQGKAPFVVKFRDTSAG
                     NPVRWNWDFGDGTGSSEQNPSHLYPFEGSYDVRLTVSNQYGSDSVFKTGSTSQRGSAA
                     PVPLTTAATQQGIVTPATQAQVNGTMTSALPAATQAPLSPFVTTGAIVIGILAIAIAR
                     RK"
     gene            7075..7413
                     /locus_tag="VFC43_03550"
                     /pseudo
     CDS             7075..7413
                     /locus_tag="VFC43_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619128.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
BASE COUNT         1723 a         1911 c         1917 g         1862 t
ORIGIN      
        1 gttcggtaat atcacggtgg catacctttc ggcaagtatc ttcctgttcg gcggagctct
       61 gaacggctgg gacgggctga tccatatcat cccgatcgcg atgattacgt tctttgccat
      121 gctgtccaga gaactggtga aggatgcgga ggatgtatac ggtgacaagg cgggagacgc
      181 agatactgtt ccgatccggt ttggcattaa aaaaacggca tatcttgctt tttttttcac
      241 ggtaattgcg gcgctggcaa gtgtcatccc gtacatgtgg tgggggatat ggtatcttgc
      301 aggaatcctg ctcgtcgata cgattctcct catcgctgta ttccgcgggc ttgcgtgtga
      361 cacgcccgaa tgcatgaagg catcaggggc ttcacagctc ttaaaagccg ggatgttcac
      421 ttccctggtt gtttttaccc tttctgctgt atttttgtga acgaaaaact aaaaaaaaag
      481 aacagataac cgagcaacag gtttatagcc tgatccttcc tcaacgtatc ctgaggagta
      541 atgccaacag ttatccagaa aggggatacg ttctttgcac aaaaaggggc gtatttcgaa
      601 ggaaatgtga agatcgatgg gaatttcatc gttcccgccc ggacacattt ctggggacgg
      661 ctggtcgtca ccggaacgct ggagatgggc ccggggtcca gcgttgcgct tggtgtagac
      721 tgctggaatg cgatcattgg cagccataca aagattaaag ggccgttggt ggcgcacggg
      781 gatgtaaccc tgttggacca tgcggtagtc cactcggtga aagcaggagg aaaggtaatc
      841 ctgcgcacgg gagtacaggt gggtgatgtc acgagcagcg atactatcat tgtccacgga
      901 aaaataaaaa gcgggaaatt agtcggaaaa catatgaagg tcctcgggaa ttgataaagg
      961 atcaatccca agggcagcta cgttatagac atctgcccag ttgacaagcg tttccacgca
     1021 tccttccttt agtgtcacga cacccataac aaccagtccc atcttgtcag ccatatcgat
     1081 gccctctttt acttcagaaa ggcagccgac accgatgatc gccttcgggt gatacttctt
     1141 caccatccgt ttgataaaag aagagccggg gacgatgaag atccgatagc ccattttttc
     1201 aagattgaac ctgacctgcc cgatcgtgca ctgaccgcag ctccggcatt tcagcccctc
     1261 gggcgtgagg tgtgcagggc accgcgagga gcgcaggcac tggggcataa aaattgctcg
     1321 ttccggcacc gggatccgtt cgaatgccgc cgtattcatc gcgttgtgga gtttgattaa
     1381 aaatgtaagc atttcccggt cttcaagccc gaggagccgg aagaaggctt tcatcagccc
     1441 ttcgaagaag acaagtcctg acctgataag ggtggggaaa tacagccgtc ctttctttat
     1501 cgaatataac gagatgatga ccaggacaaa tgccgtgatc agggctccca ctatgatgag
     1561 cactgttatc tccccgatca ggtacatcag gttattccag gtcagaaaat caaaatccat
     1621 tgctctgcag ttgaaacggt ttagtgtatt attgtgtgtt cttcaaagat aatattgagc
     1681 cggtgctgac aggtggaagt atcacaccgc gttcggtgat tattgcggta accaggtcca
     1741 tcggggttgc atcgaatgca taatttttca cgtgtgcagc atccgggaca aacacacggt
     1801 tgcccattgt cgttacttcc tcgcgaccgc gttcttcaat aataacctcc tttccttcgt
     1861 gactcgtatc gaatgtcgac agtggtgcag caacatagaa ggggatgttg tggtaacggg
     1921 cgcagaccgc gtgcatatag gtgcctattt tgttaaagac ggcatcgtgg gtgatccggt
     1981 ctgcaccaac aataaccgca tcgatcgtgc ccgtacgcat caggtgagca gcggtagaat
     2041 cggtgataac ggttacctca ataccgtccc gggccagttc ccacgcggtg agccgggccc
     2101 cctgcaggag aggacgggtc tcacatgcca tcacttttac ccgtttaccc ccgctgaccg
     2161 cggaacggat gacccccagt gccgtacccc atgaagaaca ggcgagtgca ccggcattac
     2221 agtgggtaag aacggtgcag gtatcgggaa gcaattcagc cccatgattt ccaattgcat
     2281 gacaatttgc agtatcttca cggaaaatag catcagcttc ttctaaagcg acccttcgca
     2341 cggaagggat atccttaagg ggtgcaattt ttgcaagcac cctgtcaatc ccccagccga
     2401 gatttactgc tgtcgggcgg gttgtcctga ggatctctgc atccttccag acttcagaaa
     2461 aaaattcatt gagctcgttt ttccaagaga ccctggttgc aagtgcaata ccgtatgctc
     2521 ccgctacacc gagcgccggt gctccccgga tctcaagccg ccttattgca gcggcaaggt
     2581 gtccgatggt atggcattca gtgacagtat atctgcccgg aagaactgtc tggtcgatgt
     2641 agtggaggct ttcggttgtg gcatcccacc agagtgtagg ggccttgttc actcggatcg
     2701 tgtcctgaca gcacccttgt gttgccgcag tgctactggt tcttcagatg caatactgtc
     2761 cgccatttca ggtatcctga ccgcatccat gtatgcccgc attgccgccg atcctccgga
     2821 ggcaacactg tcagggatat cccggggtgc tgttgccgtg ccggcaaggt atatcccggg
     2881 acggattgtt gcgactgtgt ccattgcgtc gtgaacgggt ttgataaatc ctgtgtcttc
     2941 gagtgggatc ccgaagcgtt ctgcgatctg tgcggttttt tctgccgggc ccatacccac
     3001 agaaagcacg acaagttccg ggtggagcac aaggacttct gaggtctcgg aattttcgac
     3061 ctgcatcgtc atcccgttcc tgtccttgta tacatcggag ggcatgcccc ggataaactt
     3121 caccccgttt gcctttgccc gttcataata ttcttcgtat cctttgccat aggaccggat
     3181 gtccatatag cagacaatca cttcggtttc gggatgtttt tccttgatca gcagggcatt
     3241 tttaatcgcc tgcatgcaac agacgcagga acagtagggg cgcttgatgg tcatgtcccg
     3301 tgaacccacg cactggatga aaatcaccga gcggggtgtt tttccattgc tcatgcgttt
     3361 tatttttccg ccggtggggc cgctggcatt gatcatccgc tccagttcga ggctcgtgat
     3421 cacatcggag agcaggaggt gaccgaactg gggttttttc tgcgcatcga aaacggcata
     3481 ccccgtgctg attaccacac ttgctgcccg gatgaggatc tcttttggga catcctcatg
     3541 aaggactgaa cccgggccgc aggcatcata gcagagcccg cactctatgc agtgatcagg
     3601 atccttgatg acaatatccg gcacagactg gggctggggt ttgtagatcg ctttcctgac
     3661 accgatacct gcatcgaacc ggttatacac ctcaacaggg cagatctgta tacagtcccc
     3721 gcagccgtta caggtcgatt cgtccacaaa ccgggggtgc ttttttaccg ttaccgtaaa
     3781 gttgcctacc gtcccttcta tcttttccac ttctgcacag gtatacaggg taatattttt
     3841 gttccgctgg acatcgacca tcttggggga gaggatgcac atcgagcagt cgtttgtggg
     3901 aaacgtttta tccagctgcg ccatgtgccc tccgattgac ggttcacgtt cgattaagtg
     3961 cacatgtatt ccatgcccgg caatatcgag ggctgcctgg atcccggcga ctcctgcccc
     4021 aataacgacg acatcaccca tggtggctgt actccgttgc gagctcaatg taggaagccg
     4081 cattattgag gatatgctgg tgctggtctg aacctgtctg ctttactatc ttgccaggca
     4141 caccgataac aactgaatcc ggcggaatta ctttgccttc cgtgataacg gcacccgccc
     4201 cgatgatgga accgtcgccg atatgtgccc cgttgataat aatcgctccc atcccgataa
     4261 gcacccggct tccgatagta catccatgca ggatcgcccc atgacctacc gaaacatccg
     4321 taccaatagt gaccgggaat cccttactgg tatgcactac acagttgtcc tgaatattcg
     4381 accggtcacc gatcacgatc ctgtccttat cagcacggat aactgcgcca aaccagatcc
     4441 cgaccttttc tccgatcgta acatccccga tgatagtggc atttccggca gcaaaaagcg
     4501 gcacgccgga gacctgaaca tccatacgca taataacata ggaaattaag aatcaaactg
     4561 tatgaaggtt atggtaggag gcacgttcga tccccttcac gatgggcata aacacctcct
     4621 gaaacgttca tttgaggttg ccgggcccgg tggccacgtg acaatcggcc ttaccagtga
     4681 taattttgca agccggaaaa atcaccctgt acgcccgttt gccctgcgtc aggcagagct
     4741 cgaacggttt atctcattca caataccttc aacccactgg gatgtcgaac cactgaatga
     4801 ccggtatggt tctgcactgg atgcagattt cgacgcactc atcgtgtctg aagagacgct
     4861 cccggccgcc attgaaatca ataaactcag gcgtgaaaac ggtaaaaaaa aggtagacat
     4921 tcaccagatc agctgtgttt tagccgaaga cgggcgctgg atctcaagca cccggatcta
     4981 tcgtcgtgag attgatgtcc acgggcatct cctgcattga cctgcagggc gggccgataa
     5041 actttttcag cgtggacttg ttttccccgg atgattttcc tacctgccct ctgaaaatca
     5101 tttcataatc cgttcattta actgcaggac tccacaacga atccgcctgc cggtaaaaaa
     5161 ggataaaaaa gattgttgaa aaaaatgctt atttccggcg tgcaattgcg attgccagta
     5221 tcccgataac tattgcacct gtcgtgacga acggggacag cggggcctgg gtagcagccg
     5281 gaagagcgga ggtcatcgtt ccatttacct gggcctgcgt tgcgggagtc acgattccct
     5341 gttgagtcgc agcagtcgtt aacgggactg gtgctgcact gccccgctgg gaagtacttc
     5401 ctgtcttgaa gacactgtcc gaaccatact ggtttgatac cgtcagcctg acatcatagg
     5461 acccttcgaa cgggtataga tgggaggggt tctgttctga tgaccccgtt ccatcgccaa
     5521 agtcccagtt ccagcgtacc ggattgccgg cgctggtatc gcggaatttc acgacaaatg
     5581 gtgccttgcc ctggcgactg tcgacagtaa attcagcaat gggagactgg gtgaccgtga
     5641 tataatccac cttgctcaag gtgtcttccc catagctgtt taaggcactc agggtcacgg
     5701 taaacttgtt ggtagatgat ataccggtgg ttgtgtaggt atgcgtggga ttcttatcag
     5761 aagacagttc accgtcaccg aaatcccatt tccatcgggt cgggtttcct gttgtcagat
     5821 cggtaaaggt gacagtaaac ggcgtgactc ctttggtccg atctgccctg aattcggcat
     5881 gcggtgaatt aaccacagtg ataaagtttc tcttcgtatg actgatcgtg gtatatgcat
     5941 ccgatgcagt cagggtgaca tcataactgc ctggttcccg atagacatgg tcgggattct
     6001 gcccggttcc tgcagtaccg tcaccgaaat cccatttcca gtcgatgggg gagttggtgg
     6061 ataggtcagt gaaatgaata tactggttca cgctcacgcg ggtcttgtca gctttgaaat
     6121 caacccgggg accttccctg acggttatta aatttgtctg gaccttggta tcctggccaa
     6181 agatgttttt cacggtcaat gacacggtat atgaaccgtc gcggttgtag aagtggaggg
     6241 gaggattctg gtcacctgaa ctctggccat cgccaaagtc ccaggaccag gatgtgggat
     6301 tcccgaccga ggtatcaatg aaccggacat attgcacccg ggttccctgc tggtactccg
     6361 gaatctccgt cataaaatca gcccccggcc tgacccctac ggtaatatag ttggacttta
     6421 ccttggtatc ctggccattg gtatttgtgg ctatgaggct taccgtgtaa atccctttta
     6481 acaggtaggt gtgggagggg ttctgctcgg aagaagtagc cccgtcacca aaatcccaat
     6541 gccaggtcgt gggtgaattg accgttttat cggtaaaacg aaccgggctg ttgacgccaa
     6601 ttgttgtggt atctgcactg aaatctgttg ccggaacacc gcctgcaatg attgtctggg
     6661 aataactatc cttgttgatc ccgcgggtta cggtaagatc aacttgatag gcacctggtg
     6721 cagtataggc gtgaaccgga tttggcgaag acgcattgtt tccgtcacca aaattccaga
     6781 tccaggtttc cgggtttcct gtagacaggt cagaaaactt cacaaccagc ggcgcttttc
     6841 cctgctttgg atcagcgaaa aaattggctt taagcgcggg tatcacatga atgaggtgtg
     6901 ctcttgtaac atctgatgtc ccatactcat tcgattcagt gagggtgatg gtataatcgc
     6961 cgctcgacca gaacgtgtgg acgggatttg ggtcggatga ttcttttccg tcaccgaaat
     7021 tccatttcca ggatgtaggg taaccggtcg aacgatcggt aaattccacg gtcagacctc
     7081 aggtatggtc gcactgcaca ctcactggca tctgccctct cgctctataa tgcacggttg
     7141 cacacaattt cccccaaagg actggagctt cctgcaacgc ttgctgccgg cctgtctgat
     7201 aagggtatgg aaattatcga acatgacgat ctcagggacg taatctgtga tattgacgtc
     7261 ctctacgtca cccgaatcca gagagaacgg ttcccggact cggcgtctta cttcaacgtt
     7321 gcttcaaact atcgtattac acctgagctt ctgaccggtg caaaaaacca tcttatcatc
     7381 cttcatccgc tcccacgggt tgacgagata gac
//
LOCUS       DASRLV010000088         6578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_838105,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000088 DASRLV010000000
VERSION     DASRLV010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 6578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6578
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838105"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /gene="pcm"
                     /locus_tag="VFC43_03555"
     CDS             <1..421
                     /gene="pcm"
                     /locus_tag="VFC43_03555"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HZK30295.1"
                     /translation="PQPTDNILEIGTGCGYQAAVLAALVSKVFSVERISEVAELAKAN
                     LKKVGCKNVEVIVGDGTTGYLQKAPYDGIIITAATPSVPKPLIEQLTEGGRLVAPVGG
                     RDIQELIRLRKTDGFLSEERHGGVRFVPLIGECGWRG"
     gene            426..1034
                     /locus_tag="VFC43_03560"
     CDS             426..1034
                     /locus_tag="VFC43_03560"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZK30296.1"
                     /translation="MKIIDITRPLSQNILTYPGDIPPVYRQESHKRYVTTSLHMSTHT
                     GTHIDAPFHYLKNGDTIDNIPITSVMGTCRVLDVADAGINITANHLKGRLEGTDRLLL
                     KTSFSGIDQFDENYPSLTTDAARFITSNKIKCAGIDSPSVECFEGDGSVHRELLGQGC
                     IIIELLDLSDVDEGDYNLVALPLRLTGLDGSPARVILIDNRM"
     gene            1035..1646
                     /locus_tag="VFC43_03565"
     CDS             1035..1646
                     /locus_tag="VFC43_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30297.1"
                     /translation="MMDLIIDTVNTLETMMKNSGCEDGGVVLEVNADVKNCQYERGAC
                     MTAAFGGKSADFVTFDPVRAKTKISFMFGTPLETSRMRGAATAIVNVVTGFFCLSRVL
                     HSCPATSHTECNRQLMHEISGKRIFCIGTIPVVNAAFRNAMVTNPQDAEVVLINAEGI
                     IEPATGNIIHAAPATTRILCLGPSTAGIARVNQLELWCPYGTC"
     gene            1742..2992
                     /gene="glmM"
                     /locus_tag="VFC43_03570"
     CDS             1742..2992
                     /gene="glmM"
                     /locus_tag="VFC43_03570"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HZK30298.1"
                     /translation="MLFGSSGIRRKFDQTLIDTALRVGSALAFRSSDVVIGMDTRTTS
                     PLLVHLVTSGIIGSGGIAHTTGIAPTPSVAYYTRRVKAGCMVTASHNPEEYNGLKLFN
                     PNGSSFTQEQQVEMEELLKSRYWTDWQHQGSERSIDALTPHKNAILESVHIKPGITVV
                     ADCGNGAGCMLTPDLLATAGAKIHCINCNPSGHFARPSEPLKENLGNVGEMVKKTGSA
                     CAVVHDGDADRMMAFDNKGRYIDGDHLLMLFARYLDAKHVVTTSDASMIIEDIAEVKR
                     TPVGDTYVSEELLKWGDFGGEPSGAWIFPKISYCPDGPFAAALFCEIASEWNVSEELD
                     AMPSYPILRESFACDTARETVLALGATSPTDGIRIAKEDGWFLIRASGTEPKIRITAE
                     AKSPAKAKEMLKEGRDLVKQGKTA"
     gene            3015..4214
                     /gene="glmU"
                     /locus_tag="VFC43_03575"
     CDS             3015..4214
                     /gene="glmU"
                     /locus_tag="VFC43_03575"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sugar-1-phosphate
                     nucleotidylyltransferase/acetyltransferase"
                     /protein_id="HZK30299.1"
                     /translation="MECVVLAAGEGKRMRPLTAKRPKVMLPLANRPMMEHLVSAAHKA
                     GITEFVFVVGYGEREIRRYFGDGSEFGIHIRYAPQRQQRGTADALRSARDFISGPFLL
                     LNGDMILKTTDIADLCTCRTPCMSTSTTDHPKDYGVVLVENDIVTTLEEKSKHLRSNQ
                     INAGAYLFSPDIFDLVDRVQASSRGEFELTDALAHYISEKKLSAHKLSYWMDVGNPWD
                     MLDANLTLMANLSPDLQGIVEEGVTMHGTVIVGEGSVVKSGTYIEGPCIIGKNCRIGP
                     HAFIRGATSIGDDCHIGHCSELKNSIIMNGTKIPHFNYIGDSIIGTECNFGAGTKIAN
                     LRHDHANVRVCGKDTRRKKFGAVIGDGVQFGINCSINVGTIIGSNAQFAPNSYVSGCI
                     GENAVIR"
     gene            4220..5377
                     /gene="glmU"
                     /locus_tag="VFC43_03580"
     CDS             4220..5377
                     /gene="glmU"
                     /locus_tag="VFC43_03580"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sugar-1-phosphate
                     nucleotidylyltransferase/acetyltransferase"
                     /protein_id="HZK30300.1"
                     /translation="MMQAVILAAGEGKRVRPLTRSRPKALLPVANRPIIEYVIEALLK
                     NGIRDIVVVVGYRKEQVIRFLNELEVPITVVVQEKQLGTAHALNCAKSHITGDFLLLP
                     GDNYIDPHSIGRIKDIRNAMLIKDHPSPSNFGVVLLKDGLVTRIIEKPEHSPSFMVST
                     GIFSLTKDFFSFISENNITDVVSSMLAAGERLQAVVAEDWQDAIYPWDLLKMNLRLLK
                     DLPEARDGSISRQTIIHGPVKIGKGTTIGPNTIISGPVIIGNDCEIGPNCCIMPNTSI
                     GSRVKVEPFTLIGKAIVMDDTSIGSHSRIIDTVIGQGCRLADHSTITSSASLMTIEGA
                     VIKPEFGAVFGDQVVAGPFTIFNNCIVGNNVSIEGGSRIISRILPDESKVI"
     gene            5379..>6578
                     /gene="glmS"
                     /locus_tag="VFC43_03585"
     CDS             5379..>6578
                     /gene="glmS"
                     /locus_tag="VFC43_03585"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HZK30301.1"
                     /translation="MCGIVGYIGRREAAPIIVEGLKKLEYRGYDSFGVATISGDIKLD
                     KHQGRISDLSGSAVCLKGKIGIGHTRWATHGVPNDVNAHPHMDCSGRIAVVHNGIIEN
                     YAELKRQLISRGHTFRSETDTEVVAHLVEEAYAAEKNLLSAVQKVVPKLTGSYALLII
                     TVDEDRIIAARYASPLVLGIGDGEFFAASDMTPVIEHTERAIFLEDGDIASLSHEKME
                     IYHEGKPVSRPLELIDWSGEDTKKGGFPHYMLKEIYEQPQAFYNSVRSVTAETLPASV
                     KNAEAVTVIACGSSYNAGLIFKYLLEDSCAIPVQLDFASEFKYFPPPVRGLIIGISQS
                     GETADTISAIKQAKVHNCTSLAITNVLGSSISRTADHTLFMRAGPEISVAATKSFIAQ
                     LAVLMQIT"
BASE COUNT         1763 a         1517 c         1693 g         1605 t
ORIGIN      
        1 gccccagcca acagataata ttcttgagat aggaactggc tgcggttacc aggctgctgt
       61 tttggccgcc ctcgtcagca aagtattttc tgtcgagaga atatcggaag ttgcagagtt
      121 ggcgaaagca aacctcaaaa aggttggttg taagaatgtt gaagttatag tgggtgatgg
      181 gacgaccgga tacctgcaaa aagcccccta tgacggtatc atcattacag ctgctacgcc
      241 atcagttccc aaaccgctta tagaacaact cactgaaggg ggcaggcttg tggcacccgt
      301 gggcgggcgc gatatccagg agctcataag gctcagaaaa acggatggat tcctttctga
      361 agaacgccac ggaggagtca ggttcgttcc actgataggg gaatgcgggt ggagggggta
      421 gttctgtgaa aattatcgat ataacacgcc cactctctca gaacatcctc acatatcctg
      481 gtgacatacc tccggtatac cggcaggaga gtcacaaacg ctatgtgacc acatctttgc
      541 atatgagcac acatacgggt actcacatcg atgctccgtt ccattacctg aaaaacgggg
      601 acacaataga caacattccg atcaccagtg tcatgggaac ctgcagggta ctcgatgtcg
      661 cagatgcggg tataaacatc acggcaaatc acctgaaggg gcggcttgag ggcactgaca
      721 ggctcctttt gaaaacatct ttttcaggaa ttgaccagtt cgatgagaat tacccttccc
      781 ttactacaga tgcagcacgt ttcatcacca gtaataaaat taaatgtgcc ggaattgatt
      841 ctccatctgt agaatgtttt gagggtgatg gaagcgttca tcgagagctc ctcggccagg
      901 gatgcattat cattgaactc ctcgacctct ctgacgtaga tgagggagat tacaatctgg
      961 tagcattgcc actccgcctg acaggattgg atggttctcc tgcccgggtt atactcattg
     1021 ataataggat gtgaatgatg gatcttatca ttgatactgt taataccctc gaaacgatga
     1081 tgaaaaacag cgggtgcgag gatggcggag ttgtactcga ggtaaatgct gacgtgaaaa
     1141 actgccagta tgaacggggt gcctgtatga ctgcggcttt tggcgggaag agcgcggatt
     1201 ttgttacatt cgacccggtc agggcaaaga caaagatctc gttcatgttt ggtacaccgc
     1261 tcgaaacttc ccgtatgaga ggcgctgcaa cggcgattgt aaatgttgtt acaggttttt
     1321 tctgtctttc acgggtactt cattcctgcc cggcaacttc acacactgag tgcaacagac
     1381 aactgatgca tgaaatcagc ggaaaacgta tcttttgtat tggcaccatc cctgttgtca
     1441 atgctgcatt caggaatgcg atggtaacaa atccacagga tgcagaggtt gtcctgatta
     1501 atgctgaagg catcatagag ccggccacag gaaacattat ccatgcagct cctgcaacta
     1561 caaggatcct ttgtcttggc ccttcaacag ccggtatcgc acgggtcaac cagctcgagc
     1621 tctggtgccc ctatggtacc tgctgagaac cgttcgtaat acacgtacca aatatatgtg
     1681 acggaaaaaa ctctaccgac aaaaaaggta aactcttttt gaccgggatc caataatcat
     1741 catgcttttc gggtcttcgg gaatacgaag gaagttcgat cagacactta tcgatacggc
     1801 cttaagagtt ggatcagcat tagctttccg ttccagcgat gttgttatcg gcatggatac
     1861 ccggacaaca agtccccttc ttgttcatct ggttacatcc ggaatcatcg ggagcggcgg
     1921 catagcgcat actaccggga tcgctccgac tccttccgtg gcgtattata cgcgcagggt
     1981 aaaagccgga tgtatggtca ccgcttccca taaccctgag gaatataacg gactgaaact
     2041 tttcaaccca aatggttcat cgtttaccca ggagcagcag gtcgagatgg aagagttgtt
     2101 gaaatcccgc tactggacag actggcagca ccagggcagt gagcgttcca tagacgcgct
     2161 gactcctcac aaaaatgcca ttctggaaag cgttcatata aaacccggaa tcaccgttgt
     2221 agctgattgc gggaacggtg ccgggtgcat gcttacaccg gatctcctcg ccacagcggg
     2281 tgcaaaaatc cactgtatca attgcaatcc ttcaggtcat tttgcccgcc cttccgaacc
     2341 gttaaaggaa aatctgggta atgtagggga aatggtgaaa aaaacaggat ccgcatgtgc
     2401 tgttgtccat gacggggatg ctgatcggat gatggctttt gataacaaag gacgttacat
     2461 tgacggggat catcttctga tgctttttgc caggtatctt gacgcaaagc acgttgttac
     2521 aacaagtgat gcttccatga ttatcgaaga tatcgctgaa gtaaaaagga cacctgtggg
     2581 ggatacctat gtttctgagg aactcctgaa atggggagat ttcgggggcg aaccttcggg
     2641 agcatggatt ttcccgaaga tctcctattg ccccgatggg ccgtttgccg cagctctttt
     2701 ctgcgagatt gcatctgaat ggaatgtctc cgaggaactt gatgcgatgc cctcttaccc
     2761 catcctaagg gaatcttttg cctgcgacac ggcacgcgag actgtcctgg cactgggggc
     2821 gacgagccct actgacggta tacggattgc taaagaggat ggctggttcc tgatccgggc
     2881 aagcggtacg gagccaaaaa tccgcattac tgcggaggcc aaatcccccg cgaaagcaaa
     2941 ggagatgcta aaagaaggtc gtgatctggt aaaacagggg aaaactgctt aaatgttgta
     3001 atccgaggtt tagtatggaa tgtgtcgttc ttgccgcagg agaggggaag cggatgcgtc
     3061 ccctcacggc gaagcgccca aaagtaatgc tccccctggc caaccgcccg atgatggagc
     3121 acctcgtttc agctgcccac aaagcgggaa taacggaatt tgtttttgtc gttggctatg
     3181 gagagagaga gatccgcagg tattttgggg acggttcgga atttggaatt catatcaggt
     3241 atgcacccca gcgacaacaa cggggaacag ctgatgccct gagatctgca cgcgatttta
     3301 tctcgggacc ttttcttctt ctgaatgggg atatgatctt aaaaaccact gatattgcag
     3361 acctctgcac atgccgcaca ccatgcatga gcaccagcac gactgatcat ccaaaagatt
     3421 acggagtcgt attagtggaa aacgatatag tgaccacgct tgaagagaaa tccaaacacc
     3481 tcaggtccaa ccagataaat gcgggggcat atttattctc tccggatatt tttgatttgg
     3541 ttgacagggt tcaggcgtca agccggggag aatttgaact tactgatgca cttgcacact
     3601 atatcagcga aaagaagctg tcagcacaca agttgtccta ttggatggat gtggggaatc
     3661 cctgggatat gctcgatgca aatcttacac ttatggccaa tctttctcct gatctccaag
     3721 gaatcgtgga agagggagtc acgatgcacg gaaccgttat tgtcggtgaa gggagtgtgg
     3781 tgaagtccgg aacctatatt gaaggcccgt gcattatcgg gaaaaattgc cgcatcggtc
     3841 cacacgcctt tatccgggga gcaaccagca taggagacga ttgccatatc gggcactgtt
     3901 cagaactcaa gaactctatc attatgaacg ggacaaagat cccgcatttc aattatatcg
     3961 gggattcgat tattggcaca gaatgtaatt ttggggcagg aacaaaaatc gcaaatcttc
     4021 ggcatgatca tgccaatgtc agggtctgtg gaaaagatac ccgaaggaaa aagtttggag
     4081 ccgttatagg tgatggggtg cagtttggta tcaactgctc catcaatgtc gggactataa
     4141 tcgggagcaa cgcgcagttt gcacccaact cgtatgtatc cgggtgtatc ggcgagaatg
     4201 cagttatacg gtaggtgaaa tgatgcaggc agtaatatta gcagcaggag aaggaaaacg
     4261 tgttcggccg ctgaccagga gcagacccaa agcgctgctt ccggttgcaa accgccctat
     4321 tattgaatac gtgatagagg cgttgttgaa aaacgggata cgtgacatcg tagttgttgt
     4381 cggctatcgg aaagaacagg taatccggtt tttaaatgaa cttgaagtac ccattaccgt
     4441 cgttgttcag gaaaaacagc tgggaactgc ccatgccctc aattgtgcaa aatcacatat
     4501 taccggggat ttcctcctgc tgccagggga taactatatc gatcctcatt cgattggcag
     4561 aataaaagat atcagaaatg cgatgctgat aaaagaccac cccagcccgt caaactttgg
     4621 tgttgtcctg ttaaaagacg ggcttgttac ccggattatc gaaaaacccg aacattctcc
     4681 cagcttcatg gtaagcaccg gtatcttttc actgacaaaa gacttttttt cctttatttc
     4741 cgaaaataac atcactgatg tagtttcttc catgctcgct gcgggagaac gcctgcaggc
     4801 agttgttgca gaagactggc aggatgcaat ttacccctgg gacctcctga agatgaatct
     4861 acgccttctg aaagatttgc ctgaagcccg cgatggatcc atcagcaggc agacaatcat
     4921 tcatgggccg gtaaagatag ggaaaggtac cacaatcggt cccaatacga taatatctgg
     4981 cccggtaata atcggtaatg attgtgagat tggtcccaat tgctgcatta tgccaaatac
     5041 gagcattggt tcgcgggtca aggtagagcc attcactttg atcggaaaag cgatagtcat
     5101 ggatgataca tccattggtt cacattcgcg gatcattgat acggtaatcg ggcaggggtg
     5161 ccggcttgca gatcatagta cgattacatc gtcagcaagc ctgatgacta tcgaaggggc
     5221 ggtaataaaa cctgaattcg gcgctgtgtt tggcgatcag gttgttgcgg gaccgttcac
     5281 tatctttaac aactgtatcg tgggcaataa tgtttcaatt gagggaggga gccggatcat
     5341 ctcccgtatc ctgccagacg aatctaaggt gatataacgt gtgcggtatc gtaggttata
     5401 ttggccgcag ggaagctgcc ccgattatcg tggaagggct caagaaactg gaataccggg
     5461 ggtacgattc gtttggggtt gccactatca gcggtgatat caaactggac aaacaccagg
     5521 gaaggatatc agatttgtca ggctcggcgg tttgcctgaa aggaaagatc gggataggac
     5581 atacgcgctg ggctactcac ggtgtgccga atgatgtaaa tgcccacccc cacatggact
     5641 gttcaggccg aattgccgtg gtgcacaacg ggattattga aaactacgcg gagctcaaac
     5701 gtcagctgat ttcacggggc catacattcc gctctgagac tgataccgag gttgtcgcac
     5761 accttgttga agaggcatac gcagcggaaa aaaatcttct ttctgctgta cagaaggtcg
     5821 tgccaaaatt aacgggttcc tatgctctgc tgatcatcac tgtagatgaa gaccgcatca
     5881 tcgctgcccg gtatgcaagc ccgctggtac ttggaatagg tgacggggag ttttttgcag
     5941 cctcagatat gacgccggta atcgaacata cagagcgtgc aatattttta gaagacggag
     6001 atattgccag tttatctcat gaaaagatgg agatctacca tgaaggtaaa ccagtatcac
     6061 gcccgctcga gctgatcgac tggtctgggg aagacacaaa gaaagggggg tttccccact
     6121 atatgctcaa agagatctac gagcagcccc aggcatttta taattctgtc aggtccgtca
     6181 ccgctgaaac acttccggca tccgtcaaaa atgctgaagc tgtaacagtc attgcatgcg
     6241 gttcttctta caacgcagga ctaattttta aatatcttct cgaagattcc tgtgcgatcc
     6301 cggtccagct tgatttcgct tcagaattca aatattttcc tccaccggta agggggctta
     6361 ttattggaat ctcgcagtcc ggtgaaacgg ccgatacaat ctccgcaatc aagcaggcaa
     6421 aggtgcataa ttgtacaagc ctggcaatta cgaacgtact gggtagttct atcagcagga
     6481 cagcagatca cacccttttc atgcgtgccg ggcccgaaat cagtgtggca gccacaaagt
     6541 ccttcatcgc acagctcgct gtcctgatgc agataacc
//
LOCUS       DASRLV010000089         9204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_27368,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000089 DASRLV010000000
VERSION     DASRLV010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 9204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9204
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27368"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..1045
                     /locus_tag="VFC43_03590"
     CDS             257..1045
                     /locus_tag="VFC43_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30302.1"
                     /translation="MTITQGTRDRIRQVTNLVFSFAQILVTLLPALGIGSGIGDRAMA
                     SVPQVQPIYWSFFIWFLIYAGCIVYGIYQAAQTQRENNILRHIGFSTASAFIGVTAYA
                     LVAQFEIGSEWILIAIFIWILASLLYAIMCLTEDPAFLTRIEEYTVLAPISLLLGWCS
                     LAVFVNIAAILKESGILATIETPFSVLLLLIAGSVAFGIIYKTNGNIWYTIPVIWGLI
                     GVFIANTWQQQNSVVAGTAALAAVILVGILVIVPKRGYEKSDRF"
     gene            1260..1850
                     /locus_tag="VFC43_03595"
     CDS             1260..1850
                     /locus_tag="VFC43_03595"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZK30303.1"
                     /translation="MVKIQYPRYIILGLLGIKPMSGYDIKKWVDVSFRYFWDIGYGQI
                     YPTLKTLESEGLVTMKNDQAEGGPVRNLYTITAKGKTELDTWLLAPEQKEYEILLKIY
                     FGNGLPSAILKEKVRVFQKKREQELAEIERTDSYLAGLPDAMTAKPFLRSVGSCGTMT
                     YKAQIAWAEETLTMLDSVNSTGHRKTTIRKGSAGGA"
     gene            1852..2586
                     /locus_tag="VFC43_03600"
     CDS             1852..2586
                     /locus_tag="VFC43_03600"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZK30304.1"
                     /translation="MDRQKLRKSLLLISFLALPVTLFYLSPILILMGAREGVLTGSAI
                     TFAFLFIISIFAGRAWCGWICPQGALQDLVASRINHRPIDNIGRINLVKYLIWVPWMS
                     LIALLFIFSGGISAIDPLYHTDHGVSTASLFTLAIYYIIITAVFLIAAILGQRGACHS
                     VCHMAVFQIIGRKIRNLLHLPAFHMKSSSDLCNGCQRCTRECPMSLDVTAMVKSGRVE
                     HPECILCGTCADSCPKHAVGYAFGRN"
     gene            2704..3054
                     /locus_tag="VFC43_03605"
     CDS             2704..3054
                     /locus_tag="VFC43_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30305.1"
                     /translation="MMKKQIFLIAGIIILVLVVIAIYKIETNSADDRAQPEPAFNTAN
                     QITMKVIMVDENANAEYATVWLTPSARIYTLAKNITDYTIYIDLMEKSKKEGSLLTFT
                     LANDDPSVIKSINK"
     gene            3504..4043
                     /locus_tag="VFC43_03610"
     CDS             3504..4043
                     /locus_tag="VFC43_03610"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HZK30306.1"
                     /translation="MKKLFVILAILFIGILIAGCASTPPAPVVTPTPTAVPTTAAPPM
                     KNIVETAVADGRFTTLVAAVQAAKLDGALSGPGPFTVFAPTDDAFKNLPPGTVATLLK
                     TPEGQLKQILLYHVVDGKVMAADVVKLTSAKTLQGSNVNITVINGVVKINDATVIITD
                     IQTKNGVIHVIDKVLLPSK"
     gene            complement(4412..4663)
                     /locus_tag="VFC43_03615"
     CDS             complement(4412..4663)
                     /locus_tag="VFC43_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HZK30307.1"
                     /translation="MGDCKRVIEKTEKPAGYNIGFNVGAAAGQTVMHCHCHVIPRYAG
                     DTENPSGGIECLNHINGTIKQNTKEHVTGSRPATNGGSP"
     gene            complement(4642..4839)
                     /locus_tag="VFC43_03620"
     CDS             complement(4642..4839)
                     /locus_tag="VFC43_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HZK30308.1"
                     /translation="MTCYFCSPSADIIATKNSLCYARWDRNPVSRGHMLVIPFRHVQD
                     FLPCPARKNMRYSNLWATVSG"
     gene            complement(5018..5158)
                     /locus_tag="VFC43_03625"
     CDS             complement(5018..5158)
                     /locus_tag="VFC43_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30309.1"
                     /translation="MGGFLAMTMAENVSLQRNVVMCREYPQTCSAGRYSLEKIQIVVT
                     DR"
     gene            complement(5264..5920)
                     /locus_tag="VFC43_03630"
     CDS             complement(5264..5920)
                     /locus_tag="VFC43_03630"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK30310.1"
                     /translation="MTTLLEATVVHIMLAYSGLAIAVAIGIPLAVLSLRSRTSGVIIS
                     TITGIGQAIPILALVAFMVPLAGIGFMPSVLVIVVSTLLPIVRNTYAGISSVDPELAD
                     AAAGIGLTWTETVTKVRVPLSLHAIFSGIKFSSIIANGVAIVTVLIGSGGLGVIVLRG
                     LARFYVPEILIGILPAIAITLIADYCLSRLETRLTPTPLREKNPLIPHPAGTGTPANR
                     "
     gene            complement(5917..6561)
                     /locus_tag="VFC43_03635"
     CDS             complement(5917..6561)
                     /locus_tag="VFC43_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZK30311.1"
                     /translation="MDTLALIAETWQTYHLTLRTVQHLEMFALSFLITVVAGIALGIL
                     FFSSINIRVVGLSTLTVIEMIPDIALLILLIPLVGIGFPPTISAAVLYSLLPVARNTT
                     AGLRGVSPDLIETATAVGLNKRDTLYHIRIPLALPLITAGVRIAVIFCMGIVTLGGII
                     GAGGLGAPLQTGITTGNDLLILVTGCWVTFLAVGMDGIAWAISRFVSVRFGGRP"
     gene            complement(6576..7691)
                     /locus_tag="VFC43_03640"
     CDS             complement(6576..7691)
                     /locus_tag="VFC43_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZK30312.1"
                     /translation="MGRQGPANIRFENVSKRYGTITAVDHFDLEINSGELFVLIGPSG
                     SGKTNMLRMVNRLVEPDEGRILINGEDITLLDPFDLRRNIGYVIQHIGLFPHLTVSGN
                     IGLVPTLAGMPGGLLQERIRHLLSLVKLSPELFMDRYPRELSGGQQQRVGLARSLAMD
                     PPLLLMDEPFGALDPLLRHQLQQEFLGIRKQLNKTILFVTHDVEEAFRLGDRVGVVAE
                     GKLIRVGTPEDLIMNPGSSAVASLIGGEHKMRYLDHLSVKAMMIPIDPSRILDGNLFI
                     DNAREALITLRADYILVGREGVVEGIAYPQDLLNSGGKDLRLGGQAHKLPVFDSSTMA
                     SEALSAMKRQGVSIARISEPDGTGGLLVMDEIMRRLL"
     gene            complement(7717..8613)
                     /locus_tag="VFC43_03645"
     CDS             complement(7717..8613)
                     /locus_tag="VFC43_03645"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HZK30313.1"
                     /translation="MRYLTIMSVLIALSMMAAGCSQVMPSGPKTVVIGVMPFNEQYVL
                     GEMTAFLLEKGGYPTEIRSGMNNAALYEAVKSGQVDMYFDYTSSVYYQLPDRVPVDRW
                     APEEVYSIVEKGLARDGILLAGKAGFRNDNVIVVPSAWAARRNVSKVSDLAPFAKDMV
                     FGSDLVFHAAEEDGLPHVEKVYNFSFGEVRSMDPSLTFIALQSGKVDAIVAYSTDSRI
                     SLFNLTPLDDDLYAMPPYHAILLVNRERAKDTKLIAALSPLIDGIDAATMRELNSRFD
                     VDKQDPKLIARDYLVSRGLLTV"
     gene            8910..>9204
                     /locus_tag="VFC43_03650"
     CDS             8910..>9204
                     /locus_tag="VFC43_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZK30314.1"
                     /translation="MGTLGRKIVDTNVEDLVKLLNKAFSDEWLAYYQYWIGSKIVRGP
                     DKEAVIAELTLHATEELGHAVLLATRIIQLGGTPVTKPEDWYKLTNCGYDAPAV"
BASE COUNT         2397 a         2276 c         2197 g         2334 t
ORIGIN      
        1 ctgtcatgct attgactcgg tgttgatgcc gccataataa aagggtggaa tatccggatg
       61 gccccggttc aggagttact taaaatggga aaaaatggga ccctggctga acccatccca
      121 ggtatatttt cccctttaaa atcatatcct cacctcactt atttccctac ccttctcaat
      181 tgtcttcgat ttgttccatc ccgcaaatcc tatactgtag tgtattgaat atggttttgt
      241 taaagaggtg aaaatcatga caattactca aggtacacga gatcgtattc ggcaggtaac
      301 aaaccttgtc ttttcctttg cccagattct ggttacgtta ctccccgctc ttggtatcgg
      361 tagcggtatc ggtgataggg ccatggcaag tgtaccccag gtgcaaccaa tctactggag
      421 ttttttcatc tggtttttga tttacgcagg atgcattgtc tatgggatat atcaggccgc
      481 acaaacacag cgtgagaata atattcttcg tcatatcgga ttctccaccg catcagcgtt
      541 tattggcgtg actgcgtacg ctctcgttgc ccagttcgaa attggcagtg aatggatcct
      601 gattgcgatt tttatctgga ttcttgcatc gctcctgtat gcaattatgt gtctgactga
      661 ggatccagcc tttcttacaa ggattgaaga gtatactgta ctcgcaccca taagcctcct
      721 gcttgggtgg tgcagccttg cagtctttgt caatatcgct gcaatactca aagaatccgg
      781 catactggca actattgaga cacctttttc cgtacttctt ctcctcatag cagggagtgt
      841 tgcttttggg ataatctata aaaccaacgg aaatatttgg tacacgatcc cggtcatctg
      901 gggtctgatt ggggtcttta ttgccaatac ctggcaacag caaaacagtg ttgtagcagg
      961 cacggcagca ctggctgcgg tcattctcgt cgggatcctg gtaattgtgc cgaaaagagg
     1021 ttatgaaaag agcgatcggt tttaaatgtt cccggcctga ttcaaggaaa gataaatccc
     1081 aaaaaaaaat atgacgtcgc atactgggat tttatgataa tcgcgtaacc tttttgcctc
     1141 atgtgcggac ttccggtcat tcggtatcaa aaacagtcca tacctcatca ccataccttt
     1201 tatatcatat tgatacgtat cataatgata catataacaa agatatagtg gtgaacacaa
     1261 tggtaaaaat ccagtatccc cggtatatta ttctcggcct tctcggtatc aaaccgatgt
     1321 ccgggtatga tatcaagaaa tgggtcgatg tctcgttccg atatttctgg gacattggtt
     1381 acggacagat ctatcctacg cttaagacac tcgagtctga aggcctggta actatgaaga
     1441 acgatcaggc agaaggtggg ccggtcagga acctctacac gattaccgcg aagggcaaaa
     1501 cagagctcga tacctggctt cttgccccgg aacaaaagga gtacgaaatt cttctgaaga
     1561 tctattttgg caatggcctg ccttccgcta tcctcaaaga aaaagtccgg gtattccaga
     1621 aaaagcggga acaggaactt gccgagattg aaaggacgga ttcctacctc gcaggtcttc
     1681 ccgacgccat gaccgcaaaa cctttcctgc gatcggtcgg atcgtgtggt accatgacct
     1741 ataaggcaca gattgcatgg gccgaagaaa cgctgaccat gctggattct gttaacagta
     1801 ccggccaccg gaaaaccact atacgcaagg gatctgccgg gggtgcctga catggaccgc
     1861 cagaaactgc gcaaatcact gctgctgatt tcattcctcg cgttaccggt caccctgttc
     1921 tatctctcgc cgatattgat cctgatgggt gcacgtgaag gagtgctcac cggcagcgca
     1981 attacatttg cattcctgtt tattatttca atatttgcgg ggagggcatg gtgcgggtgg
     2041 atatgccctc agggagcgct ccaggatctc gtcgcatccc gtatcaacca ccgcccgatc
     2101 gataacatcg gaaggatcaa tctcgtgaag tatctcatct gggtaccatg gatgtcgctc
     2161 atcgccctcc tgttcatctt cagcggggga atatcagcga tcgatccgtt atatcatacg
     2221 gaccatgggg tctcgaccgc gagcctgttc acgctcgcca tttattatat catcattacc
     2281 gcggtctttc tcatcgccgc gatactggga cagcggggcg catgtcacag cgtctgccac
     2341 atggcagtct tccagatcat cggaagaaaa atacgtaatc tcctgcacct gccagcattt
     2401 cacatgaaat catcgtcaga cttatgcaat ggttgccagc gatgcacccg cgagtgcccg
     2461 atgagtcttg atgtcacagc gatggtaaaa agcgggaggg tcgaacaccc ggaatgtatt
     2521 ctctgcggta cctgtgcgga ctcctgcccg aaacatgcgg tgggttatgc gtttggcagg
     2581 aactaaaaac gaaccgtggt tattgagaaa gtttttgctc aggactctct ataatctatt
     2641 aattgatata tatgttttaa gggggcagtt agttcggaat tgcaccaatc acctaattaa
     2701 attatgatga aaaaacaaat atttttaatt gcaggaatta ttattttggt tttagtagta
     2761 attgccatct ataaaattga aactaacagt gctgatgata gggcacagcc agaacctgcc
     2821 ttcaatacag ccaatcaaat aacaatgaag gttattatgg tggacgagaa tgcaaatgct
     2881 gaatatgcca cagtctggct tactccgtct gctcgtatct atacgcttgc aaaaaatatt
     2941 acagactata cgatatatat tgacttaatg gaaaaatcca aaaaagaagg ctcgcttctt
     3001 acttttacgc ttgctaacga tgacccttct gtaattaaaa gcattaataa ataaaaagtt
     3061 agtttatgaa taatataatg gctagtttat ttctttttcg ataatttgat ggtctctact
     3121 taatggcgat gtaatgtccg attgagtctt gaagtcactg caatggtcaa aaagcggaag
     3181 ggtcaaacac ctggaatgca tcctctgtgg tacttgtgcc gactactgcc cgaagcatgc
     3241 tgtgagttac gcatttggct ggaattaaac cccattttac gattcacgca cattggtcat
     3301 ggatattctt ccttttctta attccggaga aaagtccggt acaatctgaa tgctggatat
     3361 aagagtactc ttttttttcc gggatcattc caacagaaat tatccggttc acccagataa
     3421 agaagggcga cgatcacggt ttttgaaaag tacagaaata tataaatatg tatataccaa
     3481 aaatccacgt tcggtgaaaa cccatgaaga aactctttgt tatcctcgcg atactgttta
     3541 tcgggattct tattgctggc tgcgcatcaa cgccaccagc accggtagtt accccaacac
     3601 cgacagcagt gccgacaacc gctgcacccc caatgaaaaa cattgttgaa acagctgtgg
     3661 cagatggacg tttcacgaca cttgtcgcag cggttcaggc agcaaaactc gatggggcac
     3721 tcagcggacc cggtccgttt acagtatttg caccaacgga tgatgcattc aaaaatttac
     3781 cgcccggtac tgttgcaacc ctgttaaaga caccagaggg acagttaaag cagattctcc
     3841 tgtaccacgt tgttgacggg aaagtaatgg ctgctgatgt agtcaagctg acttccgcaa
     3901 aaacgcttca gggatcgaac gtgaatatca ccgtgatcaa cggtgtggta aaaatcaatg
     3961 atgccacggt tataattact gatattcaga cgaaaaatgg cgtgatccac gttatcgaca
     4021 aagtcctctt accctccaag taacctgggc ctgggaatgt gatcagagac ccggtataca
     4081 attccgggtc cttatttttc agttcttcaa tgactgtaag atacacttta caccccatcg
     4141 ttgtcgatat tcttttaaaa gcggcctcat tggctcacaa aattccctcg cttgacaaag
     4201 agatgtggat gaagatccac aaagcagaaa agtaacttct cttttttatt atcgaatcgc
     4261 ttacatcatg acttccttgt tgtatagttg ggctgtaatt tcccgtaatc tatctcacgg
     4321 ttgggatttg cagggatcct tctttggtag cggtgaagga tacccctggg gaccaggagt
     4381 gagagcacca gtcaggggat aagaagactt tttatggaga cccgccattt gttgcaggtc
     4441 tggagccggt gacatgttcc ttggtattct gcttaatggt cccgtttatg tggtttaaac
     4501 actctattcc cccagatgga ttctccgtat caccagcata acgggggatc acatggcaat
     4561 gacaatgcat aacggtctgg ccggcagcag ctccgacgtt gaaaccgata ttataaccag
     4621 cgggcttttc tgttttctcg atcacccgct tacagtcgcc cacaagttcg agtatcgcat
     4681 atttttcctc gctggacaag gcaaaaaatc ctgcacatgg cggaaaggga ttaccagcat
     4741 atgtcctctg gacaccggat tccggtccca gcgggcatag caaaggctgt tctttgtggc
     4801 gattatatcg gctgatgggg aacaaaaata acaggtcatg ataggctact gtatccttat
     4861 cctctctcct atgacaaata aatacatcgt acacaaaagt caatccggtt aaaagttttt
     4921 attaatttcc tgcggcatct tttccagatt ttggatgatt ttcgtatttc atcaacagaa
     4981 atccttaccg tttcaatccc cctctgttcc ccgctgatta cctgtctgtt actacaatct
     5041 gtattttttc cagggagtac ctcccggcag aacaggtctg gggatattcc cggcacatca
     5101 ccacgttcct ttgcaggctt acattctctg ccatggtcat agcaagaaat ccacccacgg
     5161 taccattatt ctgtgaatga cccgtttttc atggaagaac gggagacaat gatgattgtg
     5221 gcagtgtccg gctgtttgca tcatggacaa ctcaaataac ctcttacctg ttcgccggag
     5281 tccccgtccc ggccgggtga ggaatgagtg gatttttctc gcggagcggg gttggggtga
     5341 gcctggtttc aagacgggaa aggcagtaat cggcaatgag agtaattgcg atggcaggga
     5401 ggattccgat aagaatttcg gggacataga accgtgccag ccctctgaga acaatgactc
     5461 caagaccccc gctcccgatt aataccgtaa ctatggctac cccgttggca atgatggagg
     5521 aaaacttaat ccctgaaaaa atggcatgca gggaaagggg aaccctaacc ttcgtcaccg
     5581 tctcggtcca ggtaagaccg atgcccgctg cagcatcagc aagctcaggg tccaccgatg
     5641 agatcccggc ataggtgttc cggacaatgg gcagcagggt gctgacaact atcacaagta
     5701 ccgacggcat aaaaccgatt cctgccagcg gcaccataaa tgccaccaga gctaatatcg
     5761 gaattgcctg cccgatcccc gtgatggtag atatgattac gcctgatgta cgggaacgga
     5821 gtgagagcac cgcgagcggg atgccgattg ctacagcaat tgcaagtccg ctgtaagcga
     5881 gcattatatg gacgactgtc gcttcaagga gtgtggtcaa ggtctccctc cgaaccgcac
     5941 agagacgaac cggctgatgg cccatgcaat cccatccata cccacggcaa gaaatgtcac
     6001 ccagcacccg gttacaagaa tcaggagatc atttcccgtg gttatccccg tctggagggg
     6061 ggcaccgagc ccgccggccc cgataatacc accgagtgtc acaattccca tgcagaaaat
     6121 aaccgcaatc cgtacaccag cggtgatcag gggcagggca aggggaatcc gtatatgata
     6181 cagggtatcc cgtttgttca ggccgactgc cgtggcagtc tcaataaggt caggactaac
     6241 tccccgtaaa cctgccgtgg tgttccgggc aaccgggagg agtgagtaga gaaccgcagc
     6301 actaatggtt ggcgggaacc caatgccgac gagcgggatg aggagaatga ggagggctat
     6361 atcgggaatc atctcgataa ccgtaagtgt cgataaaccg accacgcgga tattgatcga
     6421 agaaaagaac aggatcccaa gggcgatacc tgcaacaaca gtgatcagga aggacagggc
     6481 aaacatttcc aggtgctgca cggtcctgag tgtcaggtgg taggtctgcc atgtctccgc
     6541 aatcaaagcc agcgtatcca tattccctcg tcctgttaca acagccgtct cattatctca
     6601 tccatcacta aaagaccccc ggttccgtca ggttctgaga tacgagcgat cgatactccc
     6661 tgccgtttca tagctgataa cgcttcagaa gccatggtgg acgaatcaaa gaccgggagt
     6721 ttgtgtgcct gccccccaag ccgcaaatcc ttcccccccg agttgagaag atcctgtgga
     6781 taggcaatcc cttccacaac gccctcccgc cccacgagga tataatccgc acgtaatgta
     6841 atcagcgctt cacgggcatt atcgatgaaa agattcccgt caaggatacg ggaaggatcg
     6901 atcgggatca tcattgcctt caccgagagg tgatccagat accgcatctt gtgctctcca
     6961 ccgattaagg atgcaacggc agaagaaccc gggttcataa tgagatcttc aggtgtcccg
     7021 acccggatca gtttaccctc tgcaacgaca cccacccggt cgcccagacg gaaggcttct
     7081 tcaacatcat gggtcacaaa aaggatggtc ttgttgagct gttttcgtat ccccagaaac
     7141 tcctgctgga gctgatggcg aagcaatggg tcaagtgcac caaatggttc atccataagg
     7201 agcagcggag gatccatagc cagtgagcgg gcaaggccta cccgctgctg ctggccaccg
     7261 gagagttccc ggggatagcg atccatgaaa agttcagggg agagcttgac gagggaaagg
     7321 aggtgcctga tccgctcctg cagaagcccc ccgggcattc ctgccagtgt cggtacaagt
     7381 cctatgttgc ccgaaacggt caggtgggga aaaagaccga tatgctggat aacatatccg
     7441 atgtttcgcc gtaaatcaaa agggtcaagg agcgtgatat cctcaccatt gataagaatc
     7501 cttccttcat cgggctccac cagacggttg accatcctga gcatgtttgt cttgccgctt
     7561 ccggaagggc cgatcagaac gaaaagctca ccactattga tttccagatc gaaatggtct
     7621 actgcagtaa tcgttccata ccgtttggat acgttttcga acctgatgtt cgcaggtccc
     7681 tgtctgccca ttgccaaggg tgaaatggta ttccccctaa acagtgagca gtccgcggga
     7741 gaccaggtag tcgcgtgcga tgagtttcgg gtcctgtttg tccacatcaa accggctgtt
     7801 gagctcacgc atggttgctg cgtcgatccc atcgataagg ggtgaaaggg cggctatgag
     7861 ctttgtatcc tttgcccgct ccctgttcac cagcaggatt gcatgatacg ggggcatggc
     7921 atacaggtca tcatccagcg gggtcaggtt gaagaggctt atccgtgaat cggtcgaata
     7981 cgctacgatc gcatcaacct tgccggactg gagtgctata aaggtgagtg acgggtccat
     8041 ggacctgacc tccccaaaag agaaattata aaccttctcc acgtggggaa gaccgtcttc
     8101 ttccgcggca tggaagacta ggtcactgcc aaacaccata tctttggcaa acggggcaag
     8161 atcgcttacc ttactgacgt ttctccgggc agcccaggct gagggcacaa cgatgacatt
     8221 atcattccgg aagcctgcct ttccggccag caggatgcca tctctggcca ggcctttttc
     8281 cacgatggaa tacacctctt cgggcgccca ccggtcaacg ggtacacgat cggggagctg
     8341 gtagtagacg gaactggtat aatcgaaata catgtctacc tggccggatt ttaccgcctc
     8401 atagagggca gcgttgttca tacctgaccg gatctccgtg ggataccccc ccttttcgag
     8461 caggaaagca gtcatctcac caagtacgta ctgctcattg aacggcatca cgccaatcac
     8521 cacagttttt ggacctgatg gcatcacctg actgcatccg gcagccatca tactcagcgc
     8581 aatcaaaaca ctcattatgg tcaggtatct cacgggtatc gctcacgttg ttgtttttgt
     8641 ggatactttg ttaaggattt cggtatcttt cttctttcag ccgcaaatgt ttctttggta
     8701 tagttcccat cagtcattcg gaacgtggct gtccctgaat aaaaaaatgg cctcctgttc
     8761 ttcaaaacaa tcccgaacag tctggccttc accgttttat cgtatacgga cactacattt
     8821 tttaccctcc cgctgtcagt acggatacgc ataaaaaggt gaagtgtcga tattcaaact
     8881 gtctcttaaa atctatgtgg gtgaaaccca tgggaacgtt aggaagaaag attgtcgata
     8941 ccaatgttga agatctggta aagctcctca acaaggcatt ttctgatgaa tggctcgctt
     9001 attaccagta ctggattggc tcaaaaatag ttcgcggacc tgacaaagaa gcagttattg
     9061 ccgaactcac cctgcatgca actgaagagc tcgggcatgc tgtcctgctt gccacgcgga
     9121 ttatccagct tggtggcaca ccagtcacaa agccggaaga ttggtacaaa ctcactaact
     9181 gcgggtacga tgcaccggct gtct
//
LOCUS       DASRLV010000090        10458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_515726,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000090 DASRLV010000000
VERSION     DASRLV010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 10458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10458
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515726"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VFC43_03655"
     CDS             <1..392
                     /locus_tag="VFC43_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HZK30315.1"
                     /translation="RARFPTMNIRGIKHNLLKLLLELGRDSHPNEFVALLRENNGVID
                     ELNMVPGTITGENSASVFFDMMPLDVHLAGSAHSHPNGVLQPSDADVHFFPRAGRFHL
                     IIGYPYRSGDWRCFSADGAPCTIEVIA"
     gene            389..829
                     /locus_tag="VFC43_03660"
     CDS             389..829
                     /locus_tag="VFC43_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HZK30316.1"
                     /translation="MSVRRVVATGTFDILHPGHIYYLEESRKLGDELWVIVARDANVK
                     HKPHPIIPEEHRLQMVAALKPVSHAILGDKTDMFRPIQEIQPAVITIGFNQHFDESKL
                     RQQLGERGMNPEIVRIGKYVDDDLCSSRFVVQRIIEVRGKKDET"
     gene            1016..1663
                     /locus_tag="VFC43_03665"
     CDS             1016..1663
                     /locus_tag="VFC43_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HZK30317.1"
                     /translation="MARDISAEIDTLTEIARGRGVDARIISPEKIVVSQWVRFKCRYG
                     CKGYGKHFGCPPYAPAPDETRRMVNEYTKGLLLRFDGVPGRGAFRPEDIPDDFHDWFR
                     DLILWVNGTIHLLEKTAFYDGFYKAFGFGGYPCIYCEHQHCVAEEAPCVVDESMRRKC
                     RHMDQVRPSMEAAGMDVFATAKNVGWMLETIPCKDLEYGKIMHSDIHSIGLVLLE"
     gene            complement(1667..2809)
                     /locus_tag="VFC43_03670"
     CDS             complement(1667..2809)
                     /locus_tag="VFC43_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZK30318.1"
                     /translation="MEGIVLALFLCLVLALITKYLSVPVIPFYILAGVLLGKAGLGLV
                     TSDVISRFFSDMGLIFLLFFMGLGLKPERIASNRSAVLTSGVIDLNVNMIIGFIAAYM
                     LGFSLVESLIVASAFYISSTAMAITSLIENRKLMLRESETVIWLMVFEDLVLIIFLAL
                     ISAGNQNLVIFLLKILGVLAVLYALAHYGKEFLISILDRDDELPIIFTFAAVLTTASF
                     SMYLGIPESMMVIALGVAFATTDPDAFEQHARPFKDVFLVVFFVFFGVTIDFSGGVNW
                     FMITVICLLAIVSKLISGILTGLAIHGSALSGLEIWGNTIGRGEFSIAIAVLYGSPLV
                     GTTIAAMVIVTSIIGSFTAKYSVALRRGIMQLGRKKSPPHRVHTGR"
     gene            complement(2809..3303)
                     /locus_tag="VFC43_03675"
     CDS             complement(2809..3303)
                     /locus_tag="VFC43_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HZK30319.1"
                     /translation="MSAMSLRSLNLPGVGTKYEFQTDKGDTVAIFFTKTGTIQMYTLQ
                     KGYHTPSAAEMTPVEARRLGNILTGAIIEADQESVEIAFSALADLRVTIHSFLIPKAI
                     AGRTIEDLQIRAKTGATVIAVCRHDTNIVNPAPSFVFETGDAALVIGESDQIKMFERE
                     IMVD"
     gene            3603..5069
                     /locus_tag="VFC43_03680"
     CDS             3603..5069
                     /locus_tag="VFC43_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HZK30320.1"
                     /translation="MRTQKFTILHSNDMHGDFLAENTGESGALTEGLALLSGYINKVR
                     EEEDNVLYVIAGDMVQGSLIDSEYKGISTMEIMNYLAPDVVSLGNHELDYGLEHLLFL
                     EKIANFPIVNANLYIKKYFKRLMIPHIIIKKAGLDILFIGIITEKVMDTISRDSMIGS
                     FISIEEASREIGKICDAYRNDDIDLTVLLTHIGLDSDRELAKILDPAWGVDIIIGGHS
                     HTVLEHPVTENNILITQAGVGSDHIGRFDLMVYDDTNSIVDFSWQLVEISEKSAQPDQ
                     KLLEYIRSYQQEVDRKYNTLITKFARPLTHPCREEETSLGNLVSDIFKEVAECDIMLM
                     GSGAIRSAELGPAVTLGALRACFPFEDSLKRFTVKGSQIKRIFSHIMRPENRTGEGEC
                     YQVSEGVCAVYNGHTKTLESFCLQGEPVEESGKFTLGLIGYHVDNSTKNLDITLEELA
                     AGGPSKTVATSTIAVLEEYLRIHPNLSRKVEGRLVYIS"
     gene            5223..6101
                     /locus_tag="VFC43_03685"
     CDS             5223..6101
                     /locus_tag="VFC43_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZK30321.1"
                     /translation="MAEIPKEEFVLKCTSACAGCSDSLALRYVLKAAGPDTVLVVPAC
                     CTSVIQGIYPNTAFNIPVYNIAFGAAAACASGMSNAFRGSGKKTHVIVYAGDGGTLDI
                     GIQAMSGAFERGTDFLYICYDNEAYGNTGMQRSGGTPMGAKTTTTPTGKTDAKKDIDA
                     IIAAHNPPYMATACAAYPQDLFKKVQKALTFRGPSFIHVLAPCPPGWRFSTEKSVEMG
                     KLAVKSGMWVLYEREYGKLTINAQSKGAIKKPIPLEEYLAPQGRFKGIDAKTLEMLKQ
                     HLAKNIKKLLSEEAEQ"
     gene            6098..7210
                     /locus_tag="VFC43_03690"
     CDS             6098..7210
                     /locus_tag="VFC43_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HZK30322.1"
                     /translation="MKKIATGNKAVAEAVKQVQPAVVAAYPITPQTEIVEQIAEFVST
                     GELKSRYVPVESEHSAMAACIGASITGVRTFTATSSHGLLYMHEMTNWAAGARLPIVM
                     ANVNRSIGPGWNIWAEHTDAMQERDTGWLQVYVSTVQEAYDTTLMAFCIAEHNDVLLP
                     VMVNLDGFSLSHIMQPLETVELGDFVPQLHLPHAIDPKNPMGYGGLTGPDQHFKFRWD
                     IERSMRDSVKVIETTEKDFSKRYGRKYGFTEDYQCDDADVVVVSMGTLGKEAEVAIDI
                     LRKEGIKAGSMRLRWLRPFPKLNLQGKEVVVIDRDYSFGFGGILATSIQAQQKADVYS
                     VIAGLGGQEVTYEDIADFIRTRRIGEEFWFGVNANV"
     gene            7203..7730
                     /locus_tag="VFC43_03695"
     CDS             7203..7730
                     /locus_tag="VFC43_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HZK30323.1"
                     /translation="MYEIRIHSRGGQGGVTAARLLALSAIHDGKYATACPFYGAERRG
                     APIVSFVRIDDKPIKVYSQIKKPDLVVVLDASVMDVVDVLQGLKKEGKVLVNSTHKKD
                     FAGFSSGYVDLTGIALRENLVVAGSPILNTPVLGALAKMDIISVDSAKKSIREMFSDE
                     RNVKAAEAAYQELKI"
     gene            7727..7981
                     /locus_tag="VFC43_03700"
     CDS             7727..7981
                     /locus_tag="VFC43_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZK30324.1"
                     /translation="MSQVRQRLAMSKPTQGAAGKTGTWRVFKPVVDKEKCNQCGLCEM
                     YCPDAAIDEDLKVDLVFCKGCGICANECPKKAIEMVREEK"
     gene            8044..8775
                     /locus_tag="VFC43_03705"
     CDS             8044..8775
                     /locus_tag="VFC43_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30325.1"
                     /translation="MNAKLFGCPKDLFKYDLITDILKNFHGKIGRLVIVPMLTSYYPH
                     FRGNPGCRNKNLIECFRRFRTKEDVDKYYLTLREYFKDLKESIGTKKVRVRIEKDEMF
                     SPQTRSAYFSTIFSHFPLACLLFIDPDTGIKERDYNEKHLSFTELKAFWDQLDDESVL
                     MVGQHFQKNRVFGRTDPESKAADVIRLTSSYPLIIADNTVMFIFLTKNAQLRSELREI
                     LKNYEIKIQSIVKDPKKRHAITVTG"
     gene            complement(8809..9195)
                     /locus_tag="VFC43_03710"
     CDS             complement(8809..9195)
                     /locus_tag="VFC43_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30326.1"
                     /translation="MSEQFIFTKFRTDCYHCRQVSDQIIKAVPYKAEVACDNCGATRI
                     FIPRIEDVTKAGTFTRIGCYDMWNLLEQAGCRNCHVYGPHDVTIGCRHFTVRCRNCGF
                     THFYKFDLEYIAKDELRVGQDTIQST"
     gene            9449..9967
                     /locus_tag="VFC43_03715"
     CDS             9449..9967
                     /locus_tag="VFC43_03715"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HZK30327.1"
                     /translation="MKKIIGFIILLVVFVACAACTQQVTPEPVTTLPTTITTPVPTAE
                     ITPALTLPTTVVTTVVTTALPTATRTLSPQTKLLTTIHMKNNAFVPPELIALPGSGIT
                     WINDDTTAHSLKMSFSDGGFNTGDILPGSQWSYTFGKKEGTFNISCIYHPDMKGTVVI
                     KTTASVVGNPQK"
     gene            complement(10117..>10458)
                     /locus_tag="VFC43_03720"
     CDS             complement(10117..>10458)
                     /locus_tag="VFC43_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HZK30328.1"
                     /translation="ALEVIPRTLAENAGLDPIDMLVEIRAAHEKGKKSHGLNVFEGKA
                     VDMKAAGVVEPLRVKTQAISSAAEAAIMILRIDDVIASSKSHAPEGGMPPGGMGGMGG
                     MGGMPPGMGDY"
BASE COUNT         2851 a         2488 c         2569 g         2550 t
ORIGIN      
        1 agcgtgccag gttcccgacc atgaacatcc gaggaattaa acataatctt ctcaagctcc
       61 ttttagaact gggaagggac tcccacccca acgagttcgt ggcacttctc cgggagaata
      121 acggagttat cgacgagctg aacatggtcc ccggtaccat taccggtgaa aactcagcct
      181 cggtcttttt tgatatgatg ccgctggatg tgcaccttgc aggcagtgcc cacagccacc
      241 cgaacggtgt gctgcagcca tctgatgcgg atgtgcattt ctttccccgc gccgggcggt
      301 tccacctcat catcggttac ccgtatcgca gtggcgactg gcgatgcttt tctgccgatg
      361 gtgcaccctg tacaatcgag gtgatcgcat gagcgtccgc agggttgtcg ccaccggaac
      421 atttgacatt ctccatccgg gtcatatcta ctatcttgag gaatcaagaa aactgggcga
      481 cgaactctgg gtaattgttg cacgggatgc taatgtcaaa cacaagccgc atccgatcat
      541 tcctgaagag caccgtctcc agatggttgc cgcccttaaa cctgtatctc atgctatcct
      601 tggcgataag acggatatgt tccgcccgat acaggagatc cagcctgccg ttattaccat
      661 cggattcaac cagcatttcg atgagagtaa actacgccag cagctcggcg agcgcgggat
      721 gaacccggag attgtccgca tcgggaagta cgttgatgac gacctctgca gttcgcggtt
      781 tgtcgttcag cggatcatag aagtaagggg caaaaaggac gaaacctgat actcactttt
      841 gaatctgaca acagcagaac gccatgctga ctccactgat actttccgga caaaaagaat
      901 ctggtttttc ggcaaggcaa aagacccaca gcccggacat gattattccc acaatacgcc
      961 ggactgcaaa catttattga ttttgtgttt tcatccattc cctgagggga tgttgatggc
     1021 cagggatata tcagcagaga ttgatacact cacggaaatt gcgcgtggaa ggggtgtcga
     1081 tgcccggatt atttctccgg aaaaaattgt agtttcgcaa tgggtccgct tcaaatgccg
     1141 gtacggctgc aagggttatg gcaaacattt cggctgcccg ccctatgctc cggcaccgga
     1201 tgaaacccgc aggatggtta atgagtacac aaaaggatta ttactccgct ttgacggggt
     1261 gccagggcgg ggggcattcc gccctgaaga tataccggat gatttccacg actggttccg
     1321 ggacctgata ctctgggtta acggaacaat ccacctgctc gaaaaaactg cgttctatga
     1381 tggcttttac aaggcatttg gttttggcgg ttatccctgt atctattgtg agcaccagca
     1441 ctgtgttgct gaagaagctc cttgtgttgt ggacgagagt atgcgccgca aatgcaggca
     1501 catggaccag gtgcggccga gtatggaggc agcaggtatg gacgtgtttg caacagcaaa
     1561 aaatgtcggg tggatgctgg aaactatccc ctgcaaggac ctcgagtacg gcaagatcat
     1621 gcactccgat atccattcga ttggactggt actgctcgaa taaaggtcag cggcccgtat
     1681 gcacccggtg gggaggagat tttttccttc ccagctgcat gatccctctt cttaaggcaa
     1741 cgctgtattt tgcggtaaaa gatccaatta tcgatgttac aataaccatt gctgcaatcg
     1801 ttgttcccac gagaggagac ccatacagca cggcaatagc aatggaaaat tctccccttc
     1861 cgatggtatt tccccagatt tcaaggcctg agagtgctga gccatgaatt gccagcccgg
     1921 taagtatacc ggatataagt ttgctgacta tggcgaggag gcagattacg gtaatcatga
     1981 accagttcac acccccggaa aagtctatgg tcacgccaaa aaacacaaaa aaaaccacta
     2041 aaaaaacgtc cttgaacggg cgtgcgtgct gctcgaaggc atccggatcg gtggttgcga
     2101 atgcaacacc cagtgcaatg accatcatgg attccggtat ccccaggtac atggagaatg
     2161 atgcggtggt gagaacagct gcaaacgtga aaattatcgg aagctcatca tcccgatcaa
     2221 ggatcgagat caggaattct ttcccataat gggcgagtgc atagagaact gcaagaactc
     2281 cgagaatctt taaaaggaag atgaccagat tctggttccc tgccgaaatg agggctaaaa
     2341 agatgataag aacgagatct tcaaagacca tcagccagat gaccgtttcg gattcacgga
     2401 gcatcagctt ccggttctca atgagcgagg tgattgccat cgcagtgctc gaaatgtaaa
     2461 atgcggatgc aactatgagc gactcaacaa gagaaaatcc cagcatatac gctgcgataa
     2521 acccgatgat catattgaca ttcaggtcaa ttaccccgct tgtaaggact gccgaccggt
     2581 tcgatgcaat acgctcgggt ttcagtccga gccccatgaa aaacagtaaa aaaatcagtc
     2641 ccatatctga aaaaaaacga ctgattacat cagaagtaac caaacccagc ccggcttttc
     2701 ccagcaatac tcctgccagt atataaaagg gaataacagg aaccgacagg tacttggtga
     2761 tgagcgccag taccaggcag aggaaaagcg cgagaacgat cccttccatc aatccaccat
     2821 aatttcccgc tcaaacattt ttatctggtc gctctcaccg attaccaaag ctgcatctcc
     2881 ggtttcaaac acaaatgacg gtgcaggatt gacaatattc gtatcatgcc ggcagacggc
     2941 aataactgtt gcaccggttt ttgcccggat ctgaaggtct tcaatcgttc ttccggcaat
     3001 cgcttttggg atcaggaacg aatggatggt aacccgaaga tcggcaagcg ccgaaaacgc
     3061 tatttcaaca ctttcctggt cagcctcaat aatcgcaccg gtaagaatat tacccagcct
     3121 ccgcgcctca acaggagtca tctcagcagc gcttggcgta tgatatcctt tctggagggt
     3181 atacatctgg atggtcccgg tttttgtgaa aaaaattgca actgtatcgc ctttatcggt
     3241 ctgaaattca tattttgttc cgaccccggg aagattgagc gagcgaagag acatagcaga
     3301 cattagagaa atgatcctaa aatatattat gatatgtgac aggacatgcc gacggcacag
     3361 cgggtgcaga aaaaccaaaa taaaagagga attgttttgg tttcttcaag ggttttgaga
     3421 ggtcgggagg agagcagggg gtactgcacc tgactttttt agggttttct tcctccccat
     3481 ataccttgtt tcaacaatat tctttttaaa cgcctgccgg gcggggtgaa agtgttgctt
     3541 ttttgcataa acacgagata aaagcacaac gcataattct tttgcagcgg gatcttgtga
     3601 ttatgcgcac gcagaaattt acgattctgc attcgaacga catgcacggt gacttcctgg
     3661 cagaaaacac cggggaatca ggagcattaa ccgaggggct tgcactcctg tcgggatata
     3721 tcaataaagt ccgcgaggag gaagacaatg tcctctatgt gatcgccggc gatatggtac
     3781 agggttcact tatcgattcg gaatacaaag ggatctccac gatggagatc atgaactacc
     3841 tggctccgga tgtcgtatct ttgggtaatc atgaacttga ttatggtctt gagcacctgt
     3901 tgtttttgga aaagatagcg aatttcccca tcgtaaatgc aaacctgtac attaaaaaat
     3961 actttaaacg attgatgatc ccgcatatca tcataaaaaa agccggcctg gatattctct
     4021 tcatcggcat cattaccgag aaggttatgg acactatttc ccgggattcg atgatcggga
     4081 gtttcatatc gattgaggag gcgagccggg aaatcggcaa aatctgcgat gcttacagaa
     4141 atgacgatat cgatctgacg gtccttctca cccacatcgg gcttgactct gaccgggagc
     4201 tggcaaaaat ccttgatcct gcatggggag tcgatataat catcggcggg cattcccata
     4261 cagttcttga acatcccgtg acggaaaaca acatcctgat cacccaggcg ggggttgggt
     4321 cggatcatat cggaagattt gatcttatgg tatacgatga cacgaacagc atcgttgatt
     4381 tctcatggca gctagttgaa atttcggaaa aatccgcaca accggatcag aaattactgg
     4441 aatacatccg ctcttaccaa caggaagtcg acaggaaata caatactctg atcacaaaat
     4501 ttgcacgacc cctcacccat ccctgtcgcg aagaggaaac ctcgctggga aaccttgttt
     4561 ccgatatctt caaagaagtg gcagaatgcg atattatgct gatgggatcg ggcgccatca
     4621 gatcagctga actcggacct gccgttaccc ttggagcatt acgggcgtgt ttcccttttg
     4681 aagattcctt aaaacgtttt accgtcaaag gcagccagat aaagcggata ttttcccata
     4741 ttatgcgccc ggaaaaccgg accggtgagg gggaatgcta tcaggtcagt gaaggggtct
     4801 gtgcagtcta taatggtcat acaaaaactc tggagtcctt ttgtctgcag ggagaaccgg
     4861 tcgaagagtc cggaaaattt acccttgggt tgattgggta ccatgtcgac aacagtacaa
     4921 aaaatctgga tattaccctt gaggaacttg cagcaggggg accttcaaag acggttgcaa
     4981 catcaacgat tgcggtactg gaagagtatc ttcgtatcca tccgaatctt tcacgaaagg
     5041 ttgaggggcg gctcgtgtat atctcctgaa gaatttatcc tgcttttcca aggtcctttt
     5101 ctgggcatga aacctttttg tctgttcatg ctgatacata aagatagttt cgaaagatag
     5161 tttcgcgatc tcttcagagc gttcacgcat ctgtttcata gttaccgcag gagttggata
     5221 ccatcgcaga aatccccaaa gaagaattcg tgctcaaatg cacttcggca tgtgccgggt
     5281 gcagcgattc gctcgccctt cgctatgtgc ttaaagcagc aggaccggac actgttctcg
     5341 ttgtcccggc atgctgcacc agcgtcatcc agggcatcta cccgaacacc gcattcaata
     5401 ttcccgtcta caacatcgcg ttcggtgctg cagctgcctg tgcctcagga atgtcaaatg
     5461 catttcgggg ctccgggaaa aagacacatg taattgtata tgccggtgat ggcgggacgc
     5521 ttgatatcgg catccaggca atgtcaggtg cgtttgagcg ggggacagat ttcctgtaca
     5581 tatgctatga taacgaggca tatggcaata ccgggatgca gcggtcgggc gggacaccga
     5641 tgggagcaaa aaccaccacg actcccaccg ggaagaccga tgcgaagaaa gatatcgatg
     5701 cgatcatcgc agctcacaac ccgccttata tggccacagc ctgtgcagca tacccccagg
     5761 atcttttcaa gaaagtgcag aaagcactca ccttccgtgg accttccttc atccatgtgc
     5821 ttgcaccctg tcctcccggc tggcggttct caacagaaaa atctgttgag atgggcaagc
     5881 ttgcggtaaa atccggtatg tgggtgctgt acgaacgcga gtatggaaaa ctcacgatta
     5941 acgcccagtc aaaaggtgca atcaaaaaac caatccctct cgaagagtat cttgcaccgc
     6001 agggacgttt caagggtatt gatgcaaaga cccttgagat gctcaagcaa cacctggcaa
     6061 agaatatcaa aaagctccta tcagaggagg cggaacaatg aaaaagattg caaccggcaa
     6121 caaggcagtc gctgaagcgg taaaacaggt gcaaccggct gttgtcgccg cctacccgat
     6181 aactccccag acagagattg tcgaacagat cgcagaattc gtttcaaccg gagaactcaa
     6241 aagccggtac gttccggtgg aaagcgaaca ctctgcaatg gcggcatgta tcggggcaag
     6301 cattaccgga gtccggacat tcaccgcgac cagctcccac ggccttttat acatgcatga
     6361 aatgacgaac tgggctgccg gtgcacgtct ccccatagta atggcaaatg tcaaccgttc
     6421 cataggaccc ggctggaaca tatgggctga gcacaccgat gcgatgcagg aacgtgacac
     6481 gggatggctc caggtgtacg tgagtaccgt tcaggaagct tatgacacaa cactgatggc
     6541 attttgcata gctgagcaca atgatgtgct gcttccggta atggtaaacc ttgacggctt
     6601 ttcactcagc catatcatgc agccgcttga gactgttgag ctgggtgatt ttgtaccaca
     6661 actccatctt ccacatgcga ttgacccgaa gaaccctatg ggatatggag ggcttacagg
     6721 ccctgaccag catttcaaat tccgctggga catcgagcgc tcaatgcgcg actcggtgaa
     6781 ggtaatcgag acaaccgaga aggacttttc aaagcgctat ggaagaaaat acggattcac
     6841 cgaggattac cagtgtgatg atgcggatgt ggttgttgtt tctatgggta ccctgggcaa
     6901 agaggctgaa gtcgccatag acattctccg taaagagggc atcaaagccg ggtcgatgcg
     6961 cctgcggtgg ttgcggccat tcccgaaact taatctgcag ggaaaagagg ttgtcgtcat
     7021 cgaccgcgac tattcatttg gatttggggg tattttggca acatccatac aggcccagca
     7081 aaaagccgac gtgtacagtg taattgccgg tctggggggg caggaagtca cttatgaaga
     7141 catagcggat tttatcagga cacgtcgtat cggggaggag ttctggttcg gggtgaacgc
     7201 caatgtatga gatccgcatt cactcacgcg gcggacaggg gggggtcact gcagcacgcc
     7261 tcttggccct ttctgcaatc catgacggaa agtatgcaac agcatgcccg ttctatggcg
     7321 ctgaacgcag gggtgccccg attgtttcat ttgtcaggat cgatgataaa ccgattaaag
     7381 tgtacagcca gataaaaaaa ccagacctgg tggtagtact ggacgcgagc gttatggatg
     7441 ttgtggatgt cctgcaggga ttaaagaagg agggaaaagt cctggtaaac agcacccata
     7501 aaaaagattt tgccggattt tcgtcgggtt atgttgacct caccggcatc gcactgaggg
     7561 agaatctcgt tgtcgccggg agtcctatcc tcaatactcc tgttctgggt gctcttgcca
     7621 agatggatat tatttcggtt gattcggcaa aaaaatcaat ccgcgagatg ttctcagatg
     7681 agcgtaatgt caaggcagct gaagctgctt accaggagtt gaagatatga gtcaggttcg
     7741 tcagcgactc gcaatgagca aaccgacaca gggagcagcc ggaaagaccg ggacatggcg
     7801 tgtcttcaaa cccgttgtag ataaagagaa atgcaaccag tgcggactct gtgagatgta
     7861 ttgcccggac gcggcaattg atgaagatct caaggttgac ctggttttct gcaaggggtg
     7921 cggtatctgc gcaaacgaat gcccgaaaaa agctattgag atggtgcggg aagagaaata
     7981 aaatttcaca cccacaacct ttttttgtcc tccctgtgaa catcctcgca tgtgagggtg
     8041 tatatgaatg caaaactttt cggttgccca aaggatctct tcaaatatga tcttattacg
     8101 gatatcctca aaaatttcca tggaaaaatc ggcaggctgg tcattgtccc tatgctgaca
     8161 tcctattatc cccatttccg gggtaatccg ggatgcagga ataaaaatct cattgaatgt
     8221 ttccggcgct ttcgcacaaa agaagatgta gacaagtatt acctgaccct cagggaatac
     8281 tttaaggatc tcaaggaaag cattgggaca aagaaagtga gggtccggat tgagaaggat
     8341 gagatgtttt ccccacagac acggtcagca tatttttcca ccatcttttc ccattttccc
     8401 ctggcctgtc tgcttttcat tgatccagac acagggataa aagagcgaga ttataatgag
     8461 aagcatctct cctttactga attgaaggcc ttctgggatc agcttgacga cgaatctgtc
     8521 ctgatggtag gccagcattt ccagaaaaac cgggtgttcg ggcgaactga tccggaatca
     8581 aaagcagcag atgtcataag gctcaccagt tcatatcctc tcatcatcgc agataatacg
     8641 gtcatgttta ttttcctgac taaaaatgca caactccgtt cagagcttag ggaaatcctg
     8701 aaaaactatg aaataaagat ccagagcata gtaaaagatc ccaaaaagcg tcacgcaatt
     8761 accgtaaccg ggtaggatgc tgcaaaagat atcaaaaaat tcgtggtatc atgtggactg
     8821 aatggtatcc tgcccaaccc tgagttcgtc tttagcgata tactcaaggt cgaacttgta
     8881 gaaatgcgtg aacccgcagt tccggcacct caccgtaaaa tgccggcagc ctatggtcac
     8941 atcatgtgga ccatagacat ggcagtttct gcacccggcc tgttcaagaa ggttccacat
     9001 atcgtaacaa ccgatcctgg tgaatgtccc cgccttggtg acatcctcaa tacggggaat
     9061 aaaaatacgg gtagcaccgc agttatcaca ggcaacttca gccttgtagg gtactgcttt
     9121 gattatctga tctgaaacct gccggcaatg gtaacagtca gtgcgaaatt ttgtgaaaat
     9181 gaattgctcg ctcatgaaaa ttactggatg cgtccgggtt cattaatctg ttgcactcgc
     9241 gatcaaacct gcaagtcagg atcaggacta ccagatatcc agataaggaa aaccggaata
     9301 tcaatgctct gtgcgaagta gtatgagaat attttatgat cgtgattttg gggtttttga
     9361 atgtttttgg tatttttgct taaaataagc ctccaatcac aaaagcacta cgtaaattta
     9421 attaaaggtg actttcaatt tacttgccat gaagaaaatt attggtttta taattcttct
     9481 ggttgttttt gttgcatgcg cagcatgtac tcagcaggta acgccagaac cagtcacgac
     9541 tctgccaacc acgattacta ctcctgttcc gactgcggaa ataactcctg cactgacatt
     9601 accaactacg gttgtaacaa cggtagtaac aacagcactc cctactgcta caaggaccct
     9661 atcaccgcaa acaaaacttc ttaccacaat tcatatgaaa aataatgctt ttgttccccc
     9721 ggaactgatc gccctgccag gatccggcat cacgtggatt aatgatgata ccacagccca
     9781 ttccctgaag atgagcttct ccgacggcgg gtttaatacc ggagatattt tgccagggtc
     9841 tcaatggagt tacacatttg gtaaaaaaga gggcactttt aacataagtt gcatatacca
     9901 cccggatatg aagggtactg ttgtcatcaa gactacggca tccgtagttg gtaatccaca
     9961 gaaatagaac tatttttttt attttaaccc ggattcgttt tcactctttt cctggttaat
    10021 tttgattggt ctgcttactg gtctgcatat gctgaccata aaaagatctc aaaatgcaaa
    10081 gtatgccgga aaaatagtaa aaaaaatgag ttagtattaa tagtcgccca tgccgggggg
    10141 cataccgccc atgccgccca tgccgcccat accaccggga ggcattccgc cttctggtgc
    10201 atggctcttg gacgatgcaa tgacatcatc aatgcgcagg atcatgatag ctgcctctgc
    10261 tgcagatgag attgcctgtg tcttgacccg gagaggttcg acaacaccgg ctgccttcat
    10321 atctactgcc ttgccttcga aaacattcag gccatgtgat ttcttgccct tttcgtgtgc
    10381 tgcccgaatt tcgacaagca tatcgatggg gtcaagtcct gcattctctg caagggttcg
    10441 cgggatgacc tcaagtgc
//
LOCUS       DASRLV010000091         3740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_27932,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000091 DASRLV010000000
VERSION     DASRLV010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27932"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFC43_03725"
     CDS             <1..207
                     /locus_tag="VFC43_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HZK30329.1"
                     /translation="LFGLPALILKYINPDILEGTGFATIEEFSISPEFHTTVQENLTR
                     FKRKYPHIRVVLINRAGVIIGESI"
     gene            204..785
                     /locus_tag="VFC43_03730"
     CDS             204..785
                     /locus_tag="VFC43_03730"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HZK30330.1"
                     /translation="MKIIGVGCGPGMLTEQAIREISRAKIIYGSDRAIELARSYIPFH
                     CTVKTIDDFKKLDTLAKDAVILSTGDPMLAGLGYLTGEVIPGISSLQVAAARLHIPLS
                     RVSVVVAHGKGHEKGMKDTVDEILRGKIVFLLADPKFDVQSLYCQLSSLTVPMKIALC
                     ENLGYPEENIRVRDIQSAPQPTEALYSLVIGNF"
     gene            853..1044
                     /locus_tag="VFC43_03735"
     CDS             853..1044
                     /locus_tag="VFC43_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30331.1"
                     /translation="MTGVLMDKATKRQFSIVSLIIGIIMLFIPYLKSFDSIFPGISQY
                     FSIMFYGGLLLVVIGYYLK"
     gene            complement(1076..1600)
                     /locus_tag="VFC43_03740"
     CDS             complement(1076..1600)
                     /locus_tag="VFC43_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30332.1"
                     /translation="MPDLKSIPDDMKWRLAARLAALLPALYDDAYRGIVGEKYDEIEQ
                     NIWIDISHMVVGMVRDLSLPTRTAKEVADSLRVVMAILFGPDYKSETLEIAKSGAVVL
                     VKRCPLPASSTNSSTGDMKTFHKCMALTLTTVPQLNKNYSARFVRTMCTGDRQCEIKI
                     EEVKQPGKDDPGKK"
     gene            complement(1954..2409)
                     /locus_tag="VFC43_03745"
     CDS             complement(1954..2409)
                     /locus_tag="VFC43_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HZK30333.1"
                     /translation="MNAEDAKKIISTAIDREVEAFTFYRTVSEKAKDPALKNLFMELA
                     GEETQHREFLQGMLTKDVSKMHFEAKKDYKVVNALPSPPLTADLKPLDGIVVSIKKEL
                     EAMQMYTQLASLSTDTEQKFLFSQLANMESGHKARLEDLYTNMAFPEVW"
     gene            complement(2525..3676)
                     /locus_tag="VFC43_03750"
     CDS             complement(2525..3676)
                     /locus_tag="VFC43_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HZK30334.1"
                     /translation="MAKKGDMLYAWTNDADIKKKAELGGAVTALWKHALETKAVDAVL
                     AVQKGTDLYDAQPILVTDAKDLAQTAGSLHCGTLLLPKLVKKYLDGAKIKKIGVTVKG
                     CDVMGFLELAKREQVNLDNVVMIGVNCGGSVSPILARKMITDKFGVDPDKVHKEEIDK
                     GQFIIEYEGGHKGISIDELEEAGYGRRSNCRRCKMKIPRQADLACGNWGVIGDKAGKA
                     TFIEVCSEKGANLVSGAVAKGILLTEAANPKGLEIRGKVEGAMLKLGEKWRKKDFTAL
                     GANLWETINKETSRCIKCYSCIENCPVCFTSADDLKKDTIMIKRGELPPNSMFHLRRF
                     AHISDTCINCGQCEELCPMDIPMALFSHAIRVESDAAFEPKLGKAAYSN"
BASE COUNT          906 a          884 c          855 g         1095 t
ORIGIN      
        1 ctttttggtc tgcctgcact gatactaaag tacattaatc cagatatcct cgaagggact
       61 ggatttgcca ctattgagga attttcaatt tcacctgaat ttcatactac tgttcaggaa
      121 aacctcacca gattcaaaag aaaataccct catatcagag ttgtgctgat aaaccgggct
      181 ggtgtaataa tcggagagag catatgaaaa ttataggtgt cgggtgtgga ccagggatgc
      241 tcaccgaaca ggcaatccgc gagatatcca gggcaaaaat catctatgga tcagatcgtg
      301 caatcgaact tgcacgatcg tatattcctt ttcattgcac ggtaaaaaca atcgatgatt
      361 tcaaaaagct ggatacactt gccaaagacg cagtcatcct ttcaaccgga gatcccatgc
      421 tcgcgggact cgggtatctt accggcgagg taattccggg aatatcttct ctccaggtag
      481 ctgcagcccg ccttcacatc ccattatccc gggtatctgt agttgtagcg catggcaagg
      541 gacatgaaaa aggaatgaag gatacggttg atgagatact gcggggaaaa atcgtttttc
      601 ttctggccga tccaaaattt gatgtccagt cattgtattg tcagctttca tcgcttaccg
      661 tgcccatgaa gatcgcgctc tgtgaaaatc ttggatatcc ggaagagaat attcgtgtca
      721 gggacattca gtcagcaccc caaccgaccg aggccctcta ttcgcttgtt ataggaaatt
      781 tctgatctgc cgtcatagcg ggaatgacaa gtgcggagca ataacctttt ttcctgtcag
      841 attggatagt tcatgacagg tgtattgatg gataaagcga caaaaagaca gttcagtatt
      901 gtatcattaa ttatcgggat aattatgctc tttattcctt acctgaaatc ctttgattca
      961 atatttcccg gcatatccca gtatttcagc attatgttct acgggggact tcttctggtc
     1021 gttatcgggt actacctcaa ataatcaggt attttcttat tttagggact agtcattatt
     1081 ttttcccggg atcgtcttta ccgggttgct tcacttcttc aattttgatt tcacattggc
     1141 gatctcctgt acacattgtc ctgacaaacc gggctgaata gtttttattc agctgtggaa
     1201 ctgtcgtcag tgttaatgcc atacatttat ggaaggtttt catatcacca gtgctggaat
     1261 tggtactact cgcaggcaga ggacatcgtt tcacaaggac taccgcgccg ctttttgcaa
     1321 tttcaagggt ttcggatttg taatcgggac caaagagaat tgccatcacg acccgcaggc
     1381 tgtcggcgac ctcttttgcg gtacgcgttg gcagagaaag atcgcgaacc atcccaacta
     1441 ccatatggga gatatctatc cagatattct gctcaatctc atcgtatttc tccccaacaa
     1501 tacctcggta tgcatcatcg tagagtgccg gcagcagcgc agcaagtctg gcggcaaggc
     1561 gccatttcat atcatcggga atagatttca ggtctggcac aaagaatccc cgcttacccg
     1621 gtttttatgg ttgaaacggc atatcaattc attggttgga gcacaataaa ggggctgata
     1681 atttgacagt ccgatgcaca atcttcaatt gtttctcgca cgtaattttt cctctgcaat
     1741 cattattctt tgcaatattc atgtattatt caatggtaaa aatatcaaat tatcagaaaa
     1801 ttacagaaaa gagagttatc ttcaaaaata tcatggagat gatcaggaca tgggcgatta
     1861 gtggcctgat tctcaccttg ttacgattaa ttttttctcc agagagagta taccaaaatt
     1921 ttattttttg tagaatgggg agagaatgtg ggttcaccac acctccggga aagccatgtt
     1981 ggtataaaga tcttcaagcc tggctttgtg tccgctttcc atatttgcaa gctgtgaaaa
     2041 caggaacttc tgctctgtgt cggttgagag gctcgcgagc tgagtataca tctgcatagc
     2101 ttcaagttcc tttttaattg aaacaacaat accgtcaagt ggtttcaaat cggcggtcag
     2161 cggcggactg ggaagtgcgt ttacaacctt ataatctttt ttcgcttcaa agtgcatctt
     2221 tgatacatct ttggtgagca ttccctgcag gaattcccgg tgctgcgtct cctcaccggc
     2281 aagttccatg aaaaggttct tcaatgccgg atctttggcc ttttcagata cggtacggta
     2341 aaatgtaaat gcctcgactt ccctgtcgat ggcggttgaa atgatcttct ttgcatcctc
     2401 agcattcatt gttttttcat ctccttatca gaattcaatt aattaaaccg gtttactgaa
     2461 aacctcgttc atttgtctgt ttgcacatac aaaatactca tctgattttt catagagtaa
     2521 aaaattaatt ggagtaggct gccttcccca gtttgggttc gaaagcagca tcgctctcca
     2581 cgcggattgc atgtgagaag agtgccatgg gaatatccat cggacagagc tcttcgcatt
     2641 ggccacagtt gatgcatgtg tctgagatgt gtgcaaaacg acggaggtgg aacattgaat
     2701 tcggcggaag ttctccccgt ttgatcataa ttgtgtcctt tttcagatcg tctgcagaag
     2761 tgaaacaaac cgggcagttt tcaatacagg aatagcactt gatgcaccgt gatgtctctt
     2821 tgttgatagt ctcccagagg tttgccccca gagcagtgaa atctttcttc ctccactttt
     2881 cgccgagttt gagcattgcg ccctcgactt taccacggat ctcaaggccc ttggggttcg
     2941 ccgcttctgt cagcaggata ccttttgcca ctgctccgct tacaaggtta gcacccttct
     3001 cgctgcagac ttcgatgaag gtagctttac ctgccttgtc gccgatgact ccccagttcc
     3061 cgcaggcaag gtctgcctgg cgggggatct tcatcttaca gcggcggcag ttggacctgc
     3121 ggccgtagcc tgcctcttcg agttcgtcga tggagattcc cttgtgtcct ccttcgtact
     3181 cgatgatgaa ctgtcccttg tcgatctctt ccttgtggac cttgtccggg tcaaccccga
     3241 atttatcggt aatcatcttg cgggcgagga tcgggctgac cgaaccaccg caattgacgc
     3301 caatcatgac gacgttgtca agattgacct gctcgcgttt cgcaagctcg aggaaaccca
     3361 tcacatcaca acccttgacg gtgacaccga tcttcttgat cttagcaccg tcgaggtatt
     3421 tcttgacaag tttcgggagc aggagggtcc cgcagtggag ggaaccggca gtctgggcca
     3481 ggtccttggc gtcagtgacg agtatcggct gggcatcgta caggtcggtg cctttctgga
     3541 ccgcaaggac cgcatcaacg gcctttgttt caagtgcatg tttccagagt gcggttactg
     3601 caccgccgag ttctgccttc ttcttgatgt cagcgtcgtt ggtccatgcg tagagcatat
     3661 cgcctttctt tgccatgtta cttcgcctcc gcgatcttct ctatccgcac agaacaggcc
     3721 ttgtactccg ggatacttgc
//
LOCUS       DASRLV010000092         3513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_28827,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000092 DASRLV010000000
VERSION     DASRLV010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28827"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..722
                     /locus_tag="VFC43_03755"
     CDS             165..722
                     /locus_tag="VFC43_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HZK30335.1"
                     /translation="MFVTLHKNKNAQIVLGLLFGIMFGFLLQKGGATDYNVITGQLLL
                     TDFTVLKLMLSAVIVGMIGFHVLRHFNYVQSHAAEGSIGANVIGGLIFGVGFALLGYC
                     PGTVAGAVGAGALDALLGGMVGLLIGAGIFAEMYPRIRTRVLVWGKFPAVTVPDFLHL
                     NLWIVIGLMEIFMIGFLLLLEYFGL"
     gene            909..1634
                     /locus_tag="VFC43_03760"
     CDS             909..1634
                     /locus_tag="VFC43_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HZK30336.1"
                     /translation="MTTNVKIPINYAEAAETLKKYLKLSGSPVAFRFATKKEDIPAGM
                     QELEKTIRHCSMIGLARKEGRIFYSPAGKHECNGGAWALGLREITDSLKNGDFYYKLG
                     KFESSAACKRTIDRVPHLQTLETFATMYAPLEKTPFDPQVILIVASPWAMLKLAQSVL
                     FRLGGRINAEFAGIQSVCSDTCAQTFLNGKVNFSLGCDGSRKFSGIEDGEMVMGIPAE
                     LLPEITEALNVVAAAPGSKPGSK"
     gene            complement(1787..2143)
                     /locus_tag="VFC43_03765"
     CDS             complement(1787..2143)
                     /locus_tag="VFC43_03765"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZK30337.1"
                     /translation="MFPVATASKDGTPNVIPLGIAELVNDDTVWFVDNFMNKTLSNLR
                     VNPKIAFFVWGPDIKGCYQVKGVAALKTSGKEYDEMKAKINQKNPALPARSLVIVKIT
                     EIFECKPGPTAGVKIL"
     gene            2348..>3513
                     /locus_tag="VFC43_03770"
     CDS             2348..>3513
                     /locus_tag="VFC43_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HZK30338.1"
                     /translation="MCVLREPPVKKILYWCTTCNVPLIAKSCACGRDGKRIELLQPYD
                     VRPALAADTQLIRTLVHERFGPVPLPKIILLNKTGGVDRADLVIMHGERFGWLTFDPV
                     TRTFTLDIAPEALPYILSFVTRGIVDLDLQTDARQQQGRIGGKKIPLKTPAPEGTVIV
                     KYKNRFGTGVVKEGSVKVRELNAVLPLSPLDPGWQEAVEKNRYHLKNLERNAIRAIKQ
                     HIHDRPTANVSFSGGKDITAVLHIAKKAGVTKAFFIDTGIEFPQTVDFVRSQGVEIVR
                     KGGDFWQAVEKAGPPGKDNRWCCKLLKLHPLKLYLAGIGPCVTIQGNRWYESWNRADL
                     DETSQNPANPLQLNMSPIRNWRALEVFLYLWWQKAEINPLYDRGLERIGCYLCPA"
BASE COUNT          848 a          881 c          889 g          895 t
ORIGIN      
        1 ttggtgcacg gtgggccggg ggctgcacca gcggtcatgg gatcaatgga tcgatccagc
       61 tctcgcttgc cagcattatc acggcatgct gtttttttgc cgggggaatt gcgaccgcac
      121 tgttcatatt ccgggttatc gggtcatgaa ggaggaacga cggtatgttt gttacgcttc
      181 ataaaaataa aaatgcccag atagtgctcg ggctgctctt cggtatcatg ttcggattcc
      241 tcctccagaa agggggcgct accgattata acgtgataac ggggcagctc ctgctcaccg
      301 attttaccgt cctgaaactc atgctgtcag cggtgattgt cggtatgatt ggatttcacg
      361 tgctcaggca ttttaattac gtgcagtcgc atgctgccga gggatcaatc ggcgccaatg
      421 tgatcggcgg gctcatcttc ggtgtggggt ttgccctgct cgggtattgc ccgggaacgg
      481 ttgccggtgc ggtcggggca ggagcactcg atgccctttt gggaggcatg gtcgggctgt
      541 tgatcggggc tggaatcttt gcggaaatgt acccccggat caggacccgt gtgctggtat
      601 ggggaaagtt tccggcagtt acggtgccgg atttccttca cctgaacctg tggattgtta
      661 tcggtctgat ggaaatattc atgatcgggt ttctgctcct gctcgaatat ttcggattat
      721 aacaggaatc atcaaccttc tttttgagat caggttggaa ctccttccct ctaattttca
      781 tccggattta tcgcagaccg aaaaggcatc cttaaaaaat tcactggcac ggtcattatc
      841 tttatataat tcccgtcaca tggtctagat gtaccatctt tcatacacag gatcatgagg
      901 tcagttctat gacaacaaat gtgaaaatcc ccattaatta tgctgaggca gccgagacgc
      961 tgaaaaaata cttaaagctc tccggatctc ccgtagcatt caggtttgcg acgaaaaaag
     1021 aagacattcc ggcgggaatg caggagcttg aaaagacaat acgccattgt tccatgatcg
     1081 gtcttgcaag aaaagaaggg cggatatttt attcacctgc cgggaaacat gaatgcaatg
     1141 gcggcgcatg ggcgctgggc ctgagggaaa tcacggacag cctgaagaat ggtgatttct
     1201 actacaagct gggaaaattc gaaagttccg ctgcctgtaa gcggacgatc gaccgcgtcc
     1261 cccacctcca gacccttgag acgttcgcta caatgtatgc acctcttgaa aagaccccct
     1321 ttgacccgca ggttatcctc attgttgcaa gtccctgggc gatgctgaaa ctcgcccaga
     1381 gcgtgttgtt ccggttaggt gggcgtatca acgcggaatt tgccgggatc cagtcagtat
     1441 gttccgatac atgcgcccag acgttcttaa acggtaaagt gaatttctct ctgggatgtg
     1501 atgggtcgcg gaagttctca ggaatcgagg atggcgagat ggtcatgggt atccccgccg
     1561 agttacttcc tgaaattaca gaagctctta acgtggttgc agctgcaccg gggtcaaaac
     1621 ccggttcgaa atgaagacca tgaaattaac taaaataccc cgggatgaat ctcaaataca
     1681 ataaaaactc ctttttctct tcacttctga taaaacgctt ttttgcaaat cctgcatgtc
     1741 aatatcataa gactggtatt tacctgatgt aaaacaaaaa aaagatttaa agtattttta
     1801 ccccggccgt gggaccgggt ttgcactcga agatctcggt gatcttcaca atcacgagtg
     1861 accgtgccgg aagggccggg ttcttctggt tgatcttcgc tttcatttca tcgtattctt
     1921 ttccgcttgt cttgagagcg gcaactccct tgacctggta acagcctttg atgtccggtc
     1981 cccatacaaa gaacgcaatc tttggattta cccgcaggtt ggagagtgtc ttgttcataa
     2041 aattgtccac aaaccagact gtgtcatcat tcacaagctc cgctatcccg agagggatta
     2101 cgttcggagt tccatctttt gacgcagtgg caaccggaaa aatatcttca tctttccgaa
     2161 tgcctctttc atatcaggag ttaacttcac catggttcct tcaccactgt tcattcggga
     2221 ttccttttgt atatacttta gtgttgcaac tccccgttgc cggttccgga tcctttcttt
     2281 tattcccttc cggcaaccgc acacctatcc cccgatcgtt tctcttatct cctgaagcgc
     2341 gtaatcaatg tgtgtgttac gcgaaccacc ggtaaaaaag atcctctact ggtgcacaac
     2401 ctgcaatgtc ccgctgattg caaagagctg tgcatgtggt cgtgacggga aaaggatcga
     2461 gctcctgcag ccgtacgatg tgcgcccggc actcgcagcg gacactcagc ttatcagaac
     2521 actcgtgcac gagcgttttg gcccggtccc cctcccaaaa ataattcttc tcaacaagac
     2581 cggtggtgtg gacagggccg accttgtgat catgcatggt gaacggttcg gctggctcac
     2641 ttttgatccg gttacccgca cattcacgtt ggatatcgct cctgaagccc ttccctatat
     2701 cctgtcgttt gttacccgcg ggattgtgga tctcgacctc cagaccgatg cgagacagca
     2761 gcaggggcgg atcgggggta aaaagatacc cctcaaaact cccgcaccgg aagggacggt
     2821 tatcgtgaaa tataaaaacc ggttcgggac cggcgtggta aaagaagggt cggtcaaagt
     2881 gagggaactt aatgctgttc ttcccctctc gccactggat cccggctggc aagaggcagt
     2941 tgaaaagaac cgttatcacc ttaaaaatct ggagagaaat gcaatacggg caattaaaca
     3001 gcatatccat gaccggccga ctgcaaacgt ctcattttcc ggtggcaaag acatcaccgc
     3061 ggtcctccat attgcaaaaa aagcaggggt aacaaaagcc ttttttatag ataccggtat
     3121 tgaatttcca caaaccgtgg attttgtccg ctcacaggga gtggagatcg tcaggaaagg
     3181 aggagatttc tggcaggcgg tcgaaaaagc aggaccccca gggaaggaca accggtggtg
     3241 ctgcaaactg ctgaagcttc atccgctcaa gctgtatctt gccggaatcg ggccctgtgt
     3301 caccatccag ggaaaccgct ggtatgaatc ctggaaccgg gcagacctgg atgaaacgag
     3361 ccagaatccg gcaaaccctc tccagctcaa catgtctccc attaggaact ggcgtgcact
     3421 cgaagtattc ctctacctgt ggtggcagaa agcggagatc aacccgctgt atgacagagg
     3481 tctcgaacgc atcggctgtt acctttgccc tgc
//
LOCUS       DASRLV010000093         2569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_28921,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000093 DASRLV010000000
VERSION     DASRLV010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28921"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFC43_03775"
     CDS             <1..211
                     /locus_tag="VFC43_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2114 family protein"
                     /protein_id="HZK30339.1"
                     /translation="RKPEYILEGIIERNLFDDPVDHLVFVDDGLARGAALMGRCMNSL
                     GKPKNPIGGVRGGPCIMSRRIKIGK"
     gene            226..468
                     /locus_tag="VFC43_03780"
     CDS             226..468
                     /locus_tag="VFC43_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30340.1"
                     /translation="MADEEQLFDILIPPGVPQKIIIDISKKFDVTVVDRIEKIKFANM
                     EGDERELLAFRGKLEEMKKVETYMRDQLNAFVAEKQ"
     gene            complement(732..1640)
                     /gene="argF"
                     /locus_tag="VFC43_03785"
     CDS             complement(732..1640)
                     /gene="argF"
                     /locus_tag="VFC43_03785"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HZK30341.1"
                     /translation="MKKDFLSILDITKGELEQILAEAGHLKTLKKQGKVHDVLRGKNL
                     AMIFEKSSTRTHISFEVGMNELGGHALFLNARDMQIWRGEEIRDTARAASRYVSGLMI
                     RAYKHSTIEEFALHATIPVINGLSDLEHPCQLLADIMTIQEHFGSTRELNVAWVGDGN
                     NVCNSLILSTVLTGMNVAVATPKGYEPADNIVKKAQTLGGKVTLVRKPEQAVKDADVI
                     VTDTWISMGDDDERDERMKAFGSYTVNAALMRHASPDARVLHCLPAHRGQEIADEVME
                     GGQSLVWDEAENRLHAQKALLVRLLK"
     gene            complement(1637..>2569)
                     /gene="purD"
                     /locus_tag="VFC43_03790"
     CDS             complement(1637..>2569)
                     /gene="purD"
                     /locus_tag="VFC43_03790"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HZK30342.1"
                     /translation="GCPKYRVFYDTPEAVSFIKDHEEDLVIKPIGLTGGKGVRIMGEQ
                     VDQSGAIAYVKSLNSGVVLEERLIGEEFTLQAFVDGNHMIPMPLVQDHKRAFEGDLGP
                     NTGGMGSYSMPDHMLPFVSVTDYKKALAIMRATIAALERTKKPYKGILYGQFMNTAKG
                     PKVIEFNARFGDPEAMNVLSLLCSDLSDIVVNMVKGSLLSTQVSFEQKATVCKYIVPA
                     GYPDSPVSGDPVTNIPQQGALLYYANVEERGGRLTTLSSRTLAFVGIGPTLEEAERIA
                     EQSASSVKGNVRYRRDIGTSALLNRRITHMKELR"
BASE COUNT          630 a          644 c          643 g          652 t
ORIGIN      
        1 acgtaaaccc gagtacattc tggaagggat catcgaacgt aacctttttg atgacccggt
       61 tgaccatctt gtgtttgtcg atgacgggct ggcacgtggc gcggcactga tgggacgctg
      121 catgaactcg ctgggaaagc caaaaaaccc tattggcggt gtccgcgggg gcccatgtat
      181 tatgagccgg cggatcaaga taggtaaatg aagtggtgac agaatatggc agatgaagaa
      241 cagttatttg atattcttat tcccccggga gtaccccaga aaataattat cgacatctcg
      301 aaaaaattcg atgtgacagt agtcgacaga atagagaaga tcaaatttgc caacatggaa
      361 ggagacgaac gggaacttct ggcattccgt ggcaaacttg aggaaatgaa aaaagttgaa
      421 acgtatatgc gtgaccagtt aaacgcgttt gtcgctgaga agcaataatc tattaaaatc
      481 ctttttttaa gactctggag tattcctcgt ttttcagccc cagggaattc cttttcagga
      541 atatgcaaca caaacttact ggtccaagat aacggagggg acaggcactg ttgtcccccc
      601 ataccgcccc tgcgcattca gcgatgatga tgtattacct gtttaggagt tattgctatt
      661 gggggggagc cgaagcggtg gggtgaaact cctgagagcg tcagaatttt caaaaatgat
      721 ttctcgagct attatttcag cagccgaacc agcagcgctt tctgggcatg gagccggttt
      781 tccgcctcat cccagacaag gctctggcct ccttccatga cttcatcggc aatttcctgg
      841 ccgcggtgag ccggaagaca atggaggacc cgtgcatcag gagaggcatg acgcatgagc
      901 gctgcattta ctgtataact cccaaatgct ttcatacgct cgtcgcgttc atcatcgtcg
      961 cccattgaga tccatgtgtc ggtgacaata acgtctgcgt ccttcacagc ctgctcaggt
     1021 ttcctgacaa gcgttacttt tcctcccaaa gtctgggctt tcttcacgat attatctgca
     1081 ggttcatacc ctttcggtgt tgcaacggca acgttcatcc cggtgaggac ggtggataag
     1141 atcagggagt tgcacacgtt gttcccatct ccgacccagg cgacattcag ctcgcgggtc
     1201 gagccgaaat gctcctggat cgtcatgata tcggcaagca gctggcatgg gtgctcaaga
     1261 tcggaaagcc cgttgatcac cggaattgtg gcatgcaggg caaactcttc aatagtactg
     1321 tgcttatagg cacgtatcat caggccggac acataccgtg aggcagcccg tgccgtatcc
     1381 cggatctcct cgcccctcca tatctgcata tcccgggcat ttaaaaaaag ggcatggccg
     1441 ccaagttcgt tcataccgac ttcaaacgaa atgtgcgtcc ttgtggacga cttttcaaag
     1501 atcattgcca ggtttttgcc acgaagtaca tcgtggactt ttccctgttt ttttaaggtc
     1561 ttcaggtgtc ctgcttcggc caggatctgc tcaagctcac ctttggttat gtcaagaatc
     1621 gacaggaaat ccttcttcat cgtaactcct tcatatgagt gatacgccgg ttcaggagag
     1681 cactggtccc tatatcacga cggtaacgca cattcccctt tactgaagaa gcagattgtt
     1741 ctgcgattcg ttctgcctcc tcaagggttg gaccaatacc gacaaacgca agagtgcggg
     1801 agctcagggt ggtaagtctg cccccccgtt cttccacatt cgcatagtac aggagagccc
     1861 cttgttgggg aatatttgtg acgggatctc ctgaaacagg agaatcgggg tacccggccg
     1921 gcacaatata tttacatacc gttgcttttt gctcaaaaga tacctgtgtg gacaaaagtg
     1981 atcctttgac catgttcaca acgatatcgg acaggtcaga acagagcagg gaaagcacat
     2041 tcatcgcctc cggatcgcca aaccgggcat tgaattcaat gacttttgga ccttttgcag
     2101 tattcatgaa ctgtccgtag aggatgcctt tgtagggctt tttagtccgc tcgagagcag
     2161 cgatagtggc tcgcataatc gccagcgctt ttttgtaatc tgtaacagaa acaaaaggga
     2221 gcatgtgatc gggcatggaa taggatccca tacccccggt gttcggcccg agatctcctt
     2281 cgaatgcacg tttatggtcc tggacaagag gcatgggtat catgtggttg ccatcaacaa
     2341 acgcctggag ggtgaactcc tcgccgataa ggcgctcctc cagcacaacc ccgctgttta
     2401 aggactttac atatgcgatg gcgcctgact ggtcaacctg ttctcccata atccgcacac
     2461 ctttgccccc ggtcagcccg atcggtttta taacgagatc ttcctcgtgg tctttgataa
     2521 atgagaccgc ttccggggta tcataaaaga cgcggtattt cgggcatcc
//
LOCUS       DASRLV010000094         3075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_113708,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000094 DASRLV010000000
VERSION     DASRLV010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113708"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2753)
                     /gene="leuS"
                     /locus_tag="VFC43_03795"
     CDS             complement(<1..2753)
                     /gene="leuS"
                     /locus_tag="VFC43_03795"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HZK30343.1"
                     /translation="MNTFDLGKFEEEAHEKWNHAFESNPSNLEKFYLTVAYPYPSGAM
                     HVGHGRTYIVPDVIARFWRMRGKQVLYPMAFHVTGAPVIGISKRIAKKDPKTIHLYRD
                     LYKVPQNVLDEFTDPLTIVRHFASEYQRVMTSCGISIDWRRRFTTVDPTYSRFIEWQW
                     KHLYEAGHVIKGAHPVRYCTVDDNPVGDHDLLEGDKAEVIKFTLVMFGYGDAFIPTAT
                     LRPETIHGVTNLWANPSVTYVRALVDGSPWIISKEAAEKLELQDHTVEITEEISGQDL
                     IDKTVNHPLCGTVPVLPADFVDPDMATGLVMSVPAHAPFDYIALRDLQKAGKYTTIKP
                     VPLIKVEGFGEIPAQDAVERSGITHQMDSRMDALTQEIYSAEFSKGKLFEKYGGKPVR
                     VARDDVAALMIEKYGSKIMYEFDTRPVVCRCGNKVKVKILHDQWFLKYSDPAWKKQVS
                     DHLGEMALVPPEVRIEFDRTVDWLKDWACTRRVGLGTRFPWDTTQLIEPLSDSTIYMA
                     YYTIAHKIREIDSRLLTPEVFDYIFLGKESPYLPEKKKLEAMRKEFLYWYPYNYRFSA
                     KDLISNHLTFQIFHHVTVFPRDKLPQGMVVFGMGLLNGAKMSSSKGNVFLLEDAVCDF
                     GADTVRMFLMGSAEPWQDFDWRNELVISTKKQIERFFNTVVESKDAEGEPHDIDRWII
                     SRLQSHIERTTEALTNFQTRQALQEAYFGIETDLKWYRRRLAENSDGSRELHTLCSVW
                     VRLLAPFIPFTGEHLWKKLAGEGLVSFAPWPVTDTKLVNIRIELAEELLSRTVEDIES
                     IMKLIQITPKSITISVAPAWKQEIFLFIAKSPDRNTVIREIMTDDAMRKRGKDATDAA
                     KQCTTLIHRLPPHVVGPLAKEPIDELAVFQAAKGFLEKEFGVPVHVNQSESSQHVKSA
                     TALP"
     gene            complement(2800..2994)
                     /locus_tag="VFC43_03800"
                     /pseudo
     CDS             complement(2800..2994)
                     /locus_tag="VFC43_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
BASE COUNT          692 a          740 c          803 g          840 t
ORIGIN      
        1 ggtaatgctg tagcagattt tacatgttgg cttgattctg actggtttac atggacaggc
       61 acaccgaatt ctttttctaa gaatcccttt gcagcttgaa agacggccag ctcatcgatg
      121 ggctcttttg cgaggggacc aactacgtgc gggggcaggc ggtgaataag tgttgtgcac
      181 tgctttgctg catccgttgc atcctttccc cgtttacgca ttgcatcatc cgtcatgatc
      241 tctctgataa cggtatttcg atccggtgac ttggcgatga agaggaaaat ttcctgcttc
      301 catgcaggcg cgacggagat agtgatggat ttgggggtaa tctggataag tttcatgata
      361 gattcgatat cctctaccgt gcgggagagg agctcttcag ccagctcgat ccggatattg
      421 acaagttttg tatccgtgac gggccatgga gcgaacgaaa caagaccttc gcccgcgagt
      481 tttttccaga gatgttctcc tgtgaacggg ataaacgggg caaggaggcg gacccatacc
      541 gagcagagtg tatgtaattc cctgcttcca tcgctatttt ctgcgagccg gcggcggtac
      601 catttgagat cggtctcgat gccgaaatag gcttcctgca atgcctgccg ggtctggaag
      661 ttggtgagcg cttcagtggt tcgctcgatg tgtgattgca accggctgat tatccagcga
      721 tcgatatcat gcggttcgcc ttcagcatct ttggattcaa caactgtgtt gaaaaaccgc
      781 tcgatctgtt tttttgtaga tatcaccagt tcgtttctcc agtcaaagtc ctgccagggt
      841 tcagcgctgc ccatcaggaa catacgcacc gtgtctgccc caaaatcaca aactgcatcc
      901 tcaagcagga agacattccc ttttgacgat gacatcttgg caccgttgag gagccccatg
      961 ccaaaaacga ccataccctg cgggagtttg tcccggggaa ataccgtgac gtggtggaag
     1021 atctggaatg tgaggtggtt ggaaatcagg tcttttgccg agaaccggta attgtacggg
     1081 taccagtaca ggaattcttt tctcatcgct tcgagcttct tcttctcggg aaggtacgga
     1141 gattctttcc cgaggaagat gtagtcaaag acttcaggcg tgaggagtct tgagtcaatt
     1201 tcacggatct tgtgagcgat agtgtagtat gccatgtaga tagtcgaatc cgagagcggt
     1261 tcaatgagtt gtgtggtgtc ccacgggaac cgggtgccaa gaccgacccg ccgggtgcat
     1321 gcccagtctt tgagccagtc aacggtccgg tcaaactcaa tgcgaacttc aggaggcacc
     1381 agtgccattt ccccaaggtg gtcactgacc tgcttcttcc atgcaggatc gctgtatttt
     1441 aagaaccact ggtcatgcag gatcttgact ttaaccttgt tgccacagcg acagacaacc
     1501 gggcgggtgt caaattcgta catgatcttc gagccgtatt tttcaatcat gagcgctgca
     1561 acatcatcac gtgccacgcg gacgggtttg cccccatact tttcgaagag tttacccttg
     1621 gaaaactcgg cagagtaaat ttcctgggtg agtgcatcca tgcggctgtc catctggtgg
     1681 gtgatacccg atctctcaac tgcgtcctgt gcgggaattt ctccgaatcc ttcgaccttg
     1741 attaagggca ctggtttgat cgtggtatat tttcctgcct tctggagatc gcggagtgcg
     1801 atatagtcaa agggggcatg agcagggaca ctcatgacaa gcccggttgc catgtcagga
     1861 tcgacaaagt ctgcagggag gaccggaact gtaccacaca gcgggtggtt cacagttttg
     1921 tcgattagat cctggccgga aatctcttca gttatctcaa cggtatggtc ctgcaattcg
     1981 agcttctcag ctgcttcttt tgatattatc catggacttc catccaccag tgccctgaca
     2041 tacgttacgg acgggtttgc ccagaggttt gtaaccccgt gaattgtttc ggggcgaagg
     2101 gttgcagtgg ggatgaatgc atcgccatac ccgaacatga cgagggtgaa tttgattacc
     2161 tcggccttat ccccttcaag caggtcgtgg tctccgaccg ggttatcatc aacagtacag
     2221 taacgaaccg gatgagctcc tttgattacg tgtcctgctt catagaggtg tttccattgc
     2281 cactcgatga acctgctgta ggttggatcc acggtcgtaa acctgcggcg ccagtcgatc
     2341 gaaataccgc atgaggtcat cactctctgg tactcactgg cgaaatggcg gacaatggtc
     2401 agcggatcag taaattcatc caggacgttt tgtgggactt tgtagagatc gcggtagagg
     2461 tgaatggttt tgggatcttt ctttgcgatt cgttttgaaa taccgataac tggtgctccc
     2521 gtgacgtgaa atgccatcgg gtagagaacc tgtttacccc gcatcctcca gaaccgtgcg
     2581 attacgtcag ggacgatata tgtccgtcca tgcccgacgt gcattgcccc gcttggatac
     2641 gggtatgcga cggtcaggta aaatttttcc aggttcgacg ggttggattc aaaggcatga
     2701 ttccactttt catgtgcttc ttcttcaaat tttcccagat caaatgtgtt caaaccattt
     2761 caccttcgtg tatgtataaa aaataaaaaa aattccgggt tataccggtc ttaatttgat
     2821 aatctcactg ttctggtagc gtcggatcat ctcctgcgct atgctcgagt tcttggaaag
     2881 gtgtatctct cccaggtcat ttacggttgc agtgaacagg tattccttac ctgcaaacac
     2941 gtctacaatc ttgccgctct gttccggggc aacaatggta agctgttttt tatcagctta
     3001 ccattgttgc cccggaacag agcggcaaga ttgtagacgt gtttgcaggt aaggaatacc
     3061 tgttcactgc aaccg
//
LOCUS       DASRLV010000095         4827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_29345,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000095 DASRLV010000000
VERSION     DASRLV010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29345"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..1256
                     /locus_tag="VFC43_03805"
     CDS             21..1256
                     /locus_tag="VFC43_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     gamma"
                     /protein_id="HZK30344.1"
                     /translation="MHDVRVPSVNIGVVGHVDHGKTTLVNALTGTWTDRHSEEVKRGI
                     SIRLGYADATFYRCEKCEGPGAFSTSPDCPACGGKGTAFRSISFVDAPGHETLMATML
                     SGSALMDGAMLVIASSEKCPQPQTKEHLMALELVGIKNIVIVQSKIDVVSQKEAVDNY
                     HEIKKFIKGTIAENAPIIPVSSQKGINIGALVQALDQTIPEPVRDPNAEPVMLIARSF
                     DVNKPGCNWKDVKGGVVGGSLIRGILREGDDIEIRPGRQTQIENRIKWIPITTKVTSI
                     NAGSHKIVEATPGGLLGVGTKLDPALTKSDALAGQVLGHEGKLPPVWDKIRCRVTLME
                     RVVGATSELVIEPLKHSEPLMLSVGTAVTVGVVSNTKKDTIEIQLKRPVCAELGSRIA
                     ISRQVGARWRLIGMGVLSE"
     gene            1253..1636
                     /locus_tag="VFC43_03810"
     CDS             1253..1636
                     /locus_tag="VFC43_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HZK30345.1"
                     /translation="MKILLDANALMMPVQFQIDIFDELRSLLGAFEPFVLSGVIRELT
                     GLTRARGRDGAAARHGLVLGETCTVVESGDLKSESVDAQMIEYAVRNGCMVVTNDRYI
                     RDALLTRGVSVISMRNQKKLEILRR"
     gene            1640..2281
                     /locus_tag="VFC43_03815"
                     /pseudo
     CDS             1640..2281
                     /locus_tag="VFC43_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286812.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
     gene            2265..2471
                     /gene="spt4"
                     /locus_tag="VFC43_03820"
     CDS             2265..2471
                     /gene="spt4"
                     /locus_tag="VFC43_03820"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor subunit Spt4"
                     /protein_id="HZK30346.1"
                     /translation="MPPANKKKQGKVCRDCHRVVDGEICVICGTTNLSEDWSGYLVII
                     DPENSEVAKRMNIKLPGRYALKVR"
     gene            2471..2962
                     /locus_tag="VFC43_03825"
     CDS             2471..2962
                     /locus_tag="VFC43_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-dependent dephospho-CoA kinase family
                     protein"
                     /protein_id="HZK30347.1"
                     /translation="MLTLPEEHRTLFQKPFGELHQDIDEILPKIEGLIIYAVGDVVTH
                     NLHKRGITPAIAVVDGYTMRLPCKRMTVFPGECIKVKNPAGTLTDDLIRALDHAIRYP
                     PVTVIVDGEEDLAVIPLVIAAPNGAVVLYGQPHQGVVLRVVNHQAKDTARHFLKHFVR
                     TDV"
     gene            3067..3366
                     /locus_tag="VFC43_03830"
     CDS             3067..3366
                     /locus_tag="VFC43_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S24e"
                     /protein_id="HZK30348.1"
                     /translation="MDFEITSDKRNELLSRREVQFNLKYDGATPSRMQIIGKLCALLN
                     VKEHQITLDTLHNSFGKTEITGSARIYDTEEGRKKTERPHLAARGMPKPKEEGAA"
     gene            3369..3554
                     /locus_tag="VFC43_03835"
     CDS             3369..3554
                     /locus_tag="VFC43_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27ae"
                     /protein_id="HZK30349.1"
                     /translation="MAAAKKAAAAKGQQRGAYFKVEGAKVTTAKKYCPRCGPGVMMAD
                     HKDRNTCGKCGYTEFRK"
     gene            3561..>4827
                     /locus_tag="VFC43_03840"
     CDS             3561..>4827
                     /locus_tag="VFC43_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional N(6)-L-threonylcarbamoyladenine
                     synthase/serine/threonine protein kinase"
                     /protein_id="HZK30350.1"
                     /translation="MPFFGQILGIEGTAWNLSAALFDRDLISLASDPYSPAQGGIHPR
                     EAAQHHASAMKKLISSILKEPDKITGIAFSQGPGLGPCLRTVATSARSLALALNVPLV
                     GVNHCVAHVEIGCFDTGCHDPIVLYASGANTQVIGYLNGRYRIFGETLDIGIGNALDK
                     FARSKGLPHPGGPIIESQAKEGQYIELPYTVKGMDLAFSGLVSAAKESKASLPDVCFS
                     LQETAFAMCVEVTERAMSLAGKHEVLLVGGVGANRRLQEMLRMMCEARGAQFYVPRQK
                     YLGDNGAMIAYTGKLMLESGSSLPIESSQIKPSFRADDVEVTWKSNIPSVDSTILSDN
                     ETSKRGAEAIVTFRNGKAEKHRVAKSYRVPTLDKRLIAERTRAEARLIAAARKSGVPT
                     PIMSDITGDTIVMEKVNGTLLTRNLTDATV"
BASE COUNT         1315 a         1081 c         1324 g         1107 t
ORIGIN      
        1 agtaaggagg ttcgaatttt ttgcatgatg taagagttcc cagtgttaac atcggggtag
       61 ttggccatgt cgatcacggc aaaaccactc tggtaaacgc gctcacaggg acatggacag
      121 accggcacag tgaagaggtg aagcgtggca tctcaattcg gcttggatat gccgatgcaa
      181 ccttttaccg gtgtgaaaag tgcgaggggc ccggcgcctt ttcaaccagc cccgattgtc
      241 cggcctgcgg gggaaagggg acagcattcc gatcaatttc gtttgttgat gcaccgggac
      301 atgaaacact catggcaacg atgctttccg gttcggcatt gatggacggt gcaatgctgg
      361 ttattgcttc aagcgagaaa tgcccacaac cacagacaaa agagcacctc atggctctcg
      421 aacttgtcgg tataaaaaat atcgttatag tccagagtaa aattgatgtt gtcagccaga
      481 aagaggctgt cgataattat catgagatta aaaaatttat taaagggact atagcggaaa
      541 acgcaccgat tattccggtt tcttcccaga aaggaatcaa cattggcgct ctcgttcagg
      601 ccctcgatca gacaattcct gagccagtac gggatccaaa tgctgaacct gttatgctta
      661 ttgcccgatc atttgatgta aataaacctg gctgcaactg gaaagatgta aaaggtggtg
      721 tcgtcggggg gtctcttata cggggcatcc tccgcgaagg tgacgatatt gaaatccggc
      781 caggaaggca gacacagatc gagaacagga tcaagtggat accaatcact accaaagtta
      841 cctctatcaa tgctggttcc cacaaaatcg tcgaggcaac tcctggcgga ctccttggcg
      901 ttggaaccaa acttgatccg gcactgacga aaagtgatgc ccttgccggt caggtgcttg
      961 gtcatgaagg taaacttccc ccggtgtggg acaaaatccg gtgccgggtc acgctcatgg
     1021 aacgggtcgt gggggcgaca agcgagctgg ttattgaacc gttaaaacac agcgaaccgc
     1081 tgatgctttc cgtcggaaca gcagttacgg ttggtgtagt cagtaataca aagaaagata
     1141 ccatagagat tcagctgaag cgacctgtat gtgccgaact gggttcaagg atcgccatca
     1201 gccggcaggt gggagcacgc tggcgattaa tagggatggg agtcctgagc gagtgaaaat
     1261 ccttctggac gccaatgcac ttatgatgcc agtgcaattc cagatcgata tctttgatga
     1321 acttcgctca cttctgggag cgttcgaacc attcgtgctt tccggagtca taagggaact
     1381 caccggactc acccgcgcaa gaggtcgtga cggggccgct gcccgtcatg gtcttgtgct
     1441 tggtgaaaca tgtactgtgg tggaaagcgg ggatctgaaa tctgaatctg tcgatgccca
     1501 gatgattgaa tacgcggtac gaaatgggtg tatggtcgtt accaatgacc ggtatatcag
     1561 ggatgcactg ctcacacggg gtgtaagtgt aatatcaatg agaaatcaga aaaagctgga
     1621 gatattgcgg aggtaaaaaa tgtatcataa attgatgctt gaggataagg tgcgggtacc
     1681 cccccagcga ctgggggaaa aacttgaaaa agtgattctt gaggtcctgc aggaacagct
     1741 tgaaggcagt atcgataaag agatcgggat ctttatctgt gtgacaaaag ttcaggatgt
     1801 cggggaagga gaactcgtgc ctggtgacgg gggcgtctat tatgatgtga agtttgaagc
     1861 tgttgtgctt cgccttgcat tacaggaagt catcgagggt cttgttgtag agaccacaag
     1921 tttcggggcg tttgtcagcc tcggaccgat tgatgcgatg ctgcatgtca gccagatctc
     1981 agacgagtat ataaattatg atgagaagaa cgcccgtctc atctgtcagg aatcgaagag
     2041 gttcatcggc gtgggtgatg tggtgcgggt ccgcgttgtg acgctttccc ttaacgagcg
     2101 gtagccgcgt gacagcaaga tcgggctcac gatgcgacag gcaggactgg gtacctcgct
     2161 ctggcttgaa gatgagatga ataaggaaaa agagaaagat ctgaaatccc cggctgcccc
     2221 aaaagaagca gtcaaggaaa aaggaaagag taagaggaag gagaatgcct ccggcgaata
     2281 agaaaaaaca aggaaaagtc tgccgtgatt gtcatcgggt agttgatggc gagatctgtg
     2341 tgatctgtgg cacgacaaat ctgagcgagg actggtccgg ttatcttgtg attattgatc
     2401 cggaaaattc tgaggtggcg aaacggatga acatcaaact tccgggtcgc tatgctctga
     2461 aggttcgctg atgcttaccc ttcctgaaga gcaccggacg cttttccaga agccgtttgg
     2521 cgagcttcat caggatatcg atgagatcct cccaaaaatt gaaggcctta ttatatatgc
     2581 cgtgggggat gttgttaccc ataacctcca taagcgggga atcacgccgg caattgctgt
     2641 tgtcgatggg tataccatgc gattaccctg taagaggatg acggtatttc ccggggaatg
     2701 tatcaaagtt aaaaatccgg cagggacatt aaccgatgat cttatacgcg cgttggatca
     2761 tgcgatccgg tatccacccg taaccgttat cgttgacggt gaagaagatc tcgcggttat
     2821 tccgcttgtc attgctgctc caaacggtgc ggttgtgtta tacggacaac cgcatcaggg
     2881 ggtcgtactc cgtgtggtaa atcatcaggc aaaggatact gcccgtcatt ttttgaaaca
     2941 ttttgtccgc actgacgtgt gaccattgaa tacctgaaat aaaattgggt atttggtatt
     3001 gatttgcagt gcttctgcga ggctcatatt ataaataaca cggtttccaa taattacagg
     3061 atatcgatgg actttgagat caccagcgat aaaagaaatg agcttttatc cagaagagag
     3121 gttcagttta acctcaaata cgatggtgca accccctccc gtatgcagat tattggtaag
     3181 ttatgcgccc tgctcaatgt aaaagagcat cagataacgc ttgatacgct ccacaacagc
     3241 ttcggtaaaa cagaaattac cggttcagca cgtatttacg atactgaaga gggcaggaag
     3301 aagaccgagc gcccgcacct tgcagcgcgc ggaatgccaa aacccaagga agagggagct
     3361 gcataacgat ggccgcagca aaaaaagcag ccgccgccaa gggacaacag aggggcgcat
     3421 acttcaaggt cgagggtgcg aaagtcacca ctgccaagaa atactgccca cgctgcggac
     3481 ccggggtcat gatggcggac cacaaggacc ggaacacctg tggaaagtgt ggatatactg
     3541 aattcagaaa ataagattca atgccttttt ttgggcagat actgggaatt gaggggacag
     3601 catggaacct cagtgccgct ctttttgatc gtgacctgat ttcacttgca tccgatcctt
     3661 acagcccggc acagggcggc attcatcctc gtgaagcagc gcagcaccat gcatctgcaa
     3721 tgaagaaact catctcttct atcctcaaag agcctgataa gatcaccgga atcgcgttct
     3781 cccagggacc gggacttggg ccatgtcttc gcaccgttgc cacatctgca agatcgctcg
     3841 cgctggcact caatgtgcct ctagttggcg ttaaccactg tgttgctcat gtggagatcg
     3901 gatgttttga tacaggatgt cacgatccga ttgtcctcta cgcaagcgga gcaaacaccc
     3961 aggtaatcgg gtacctcaat ggacgctacc gcatttttgg ggagacactt gacatcggca
     4021 ttggcaatgc ccttgacaaa tttgcacgat ctaaaggact gccgcatcct ggtggtccaa
     4081 tcatagagag ccaggcgaag gagggacaat atatcgagct gccgtacacc gttaagggta
     4141 tggatctcgc attttcagga ctcgtttcag ccgcaaaaga gagtaaagcg tctctgcccg
     4201 atgtatgctt cagtctccag gagactgcat ttgccatgtg tgtggaagta actgaaaggg
     4261 ccatgtcgct tgccgggaaa catgaagtcc ttctcgtagg gggggttgga gcaaaccgca
     4321 ggttgcagga aatgctccgg atgatgtgtg aggcgcgagg ggctcagttt tacgtaccca
     4381 gacaaaaata tctcggggac aatggcgcaa tgattgctta tacggggaaa ctgatgctgg
     4441 agagcgggtc atcccttccc attgaatcct cacagatcaa gccatcattc cgcgcagatg
     4501 atgtcgaggt aacatggaag agcaatattc cgtctgtgga cagcacaata ttatccgata
     4561 atgaaaccag caaaaggggt gcggaggcaa tagttacctt ccggaacggt aaggcagaaa
     4621 agcacagggt agcaaaatcc taccgtgtgc ccacacttga caaaagactg attgccgaga
     4681 ggacaagggc agaagcacga ttaatcgctg ctgcacgtaa aagcggggtt cccacaccga
     4741 taatgagcga catcaccgga gatactattg tgatggaaaa agtgaacggt actttactta
     4801 ccaggaacct gacagacgca acagtct
//
LOCUS       DASRLV010000096         6278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_517564,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000096 DASRLV010000000
VERSION     DASRLV010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 6278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6278
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517564"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VFC43_03845"
     CDS             complement(<1..812)
                     /locus_tag="VFC43_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZK30351.1"
                     /translation="MRVRAVSKDKLREIAGETWIDHTRRPYTEGDIAWVPVRDEDTCD
                     RELSPKIRYQGKGFFMVGDIAVIHGKKPRPEEVDEIVGFRHPRGVLWIESLQDVTRTP
                     KTHVLWGETGEVQHHENGYTYFLDPGKVMFAQGNRVEKMRIAQIVHNSDTPERIADMF
                     AGIGYFAIPIAGSGASVHAMEINPVAFGYLNRNIRKNGLEDRISSSLGDCRDLLTGTY
                     DRIIMGHFDAVSLLPFALRHAREGSVIHLHSIGTVEYLIKKYVKSAGFSATI"
     gene            complement(816..1856)
                     /locus_tag="VFC43_03850"
     CDS             complement(816..1856)
                     /locus_tag="VFC43_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_function: GO:0043023 - ribosomal large subunit binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="60S ribosomal export protein NMD3"
                     /protein_id="HZK30352.1"
                     /translation="MSIQDRFCPKCGQPTDQEGLCNRCRIGNTPWFACDRRVKSTQCP
                     SCSAIKQVNTWTDTECDRTLLGPELAKTAVHFHPDVRKTSMSVTVVDNTLNRSTATIV
                     LKGLLYGSPVEDACSVELIWHKEQCDRCNRISGSYYEGIVQVRADGRPPSTFELQMSA
                     GIAQQVEDSLQAGGERLSFIADMGETREGLDITVGSQHIGTLISQGIIAQLGGRVSTH
                     PKLVGEKNGRQLYRITFSVRLPRFQKQDVVVSHGRYVEVVRVESNHLKVIDLTDGTPR
                     SVREDDIDHLIGNMRNAESALVVFADDRIMGILDPLTCQTKEVRRLKWLDARTGQHVL
                     TLRDGDQLIVVG"
     gene            complement(1853..2146)
                     /locus_tag="VFC43_03855"
     CDS             complement(1853..2146)
                     /locus_tag="VFC43_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF424 domain-containing protein"
                     /protein_id="HZK30353.1"
                     /translation="MFLKVHSSPGVGDIVAVCDRELLNTTISNGNLSVKISEAFYGNC
                     PASEEMVRDALKRAGNINLMGKRSVSIAIDMGLITLSGCIMIGDIPHAQVYSL"
     gene            complement(2195..3022)
                     /locus_tag="VFC43_03860"
     CDS             complement(2195..3022)
                     /locus_tag="VFC43_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZK30354.1"
                     /translation="MQSPKFPVTDDHIHIDPINGRGVEAAKDFLRAGGTHFFLVSKPS
                     WSLSVHPSKGTDFSAVFDETLRVTRLIEETGVVVYPVLGIHPAEINRLSERMPLTEAI
                     EVMKAGIDLAAGYVREGKAIALKSGRPHYEVSPEVWRGSNEILAHALDRAAECKCALQ
                     IHAESGPCNDVAEMARNAGFPVERVIKHYGSPDTPLHPSLIAKHEAIPQMIRERRVFT
                     MESDFMDENSRPGAVMGPKSVPRYTNQLLKDGLITEDDCFRIHAETIDRVYGVRIAL"
     gene            complement(2994..3347)
                     /locus_tag="VFC43_03865"
     CDS             complement(2994..3347)
                     /locus_tag="VFC43_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase family protein"
                     /protein_id="HZK30355.1"
                     /translation="MITERDRAIFEAGIKLGALYHQWVGTPISKTSAGSIESAIEKAV
                     ILQPFVERITVRIDRTLMTQNIFGYSELSGLMFDVEIVTRVGFTYCRARLAPENGYPL
                     MQVVECYAITEISRH"
     gene            complement(3360..5471)
                     /locus_tag="VFC43_03870"
     CDS             complement(3360..5471)
                     /locus_tag="VFC43_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minichromosome maintenance protein MCM"
                     /protein_id="HZK30356.1"
                     /translation="MDKNPELHDTDKTENWSRLLKSKYKKEIGEISREYPHKRSLYID
                     YRDVERFGKAGIALADELLENPGKVLEDVWDAIKNSQLIRIKDGKEPKGINIRFTNLP
                     KKVGIRNIRSDDINKFLSIEGILRKTTEVRPRVIEAVFKCPAGHFTIKKQKYGKFIEP
                     EGCATDGCSFKKLELLPKRSKFVDSQKLRIQESPEGLRGGEQPQTLDVDVTDDLSGMV
                     APGDRVVINGILRSMQRIVKGEKSTVFDIFLECNSIEIAEKEFEEVDINEKDEDEIKA
                     LSSDPLIYGKITHSIAPTIYGSENVKEAIALQLFGGIAKEMPDGSHLRGDIHVLLIGD
                     PGIAKSQLLRYVVKLSPRAIYTSGQSSTSAGLTATAVKDEFGEGRWTLEAGALVLADM
                     GVCAVDEMDKMQKEDRSALHEAMEQQSISVAKAGITATLRSRCALLGAANPKYGRFDM
                     YGDISDQINMPPSLLSRFDLIFIMTDQPEQKLDLAIAEHILKSHGIGELIAQHKKTPI
                     PGITDEYIQQQLKPVMPDIDPPLFRKYVAYSKRSCFPILSTEAKDALVSYYLKLRGIA
                     EQNKPVPVTARQLEALVRLAEASARIRLSNTIEQHDAERVIHIVDACLRQIAYDARTG
                     SFDIDKIATGISKEKRDIIKVIKDAIRNIGGDGKRAAKDQVVDLVVSKGFSRDKVEAG
                     IDMLVRSGEAMEPKLGILQLI"
     gene            complement(5481..>6278)
                     /locus_tag="VFC43_03875"
     CDS             complement(5481..>6278)
                     /locus_tag="VFC43_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication factor C small subunit"
                     /protein_id="HZK30357.1"
                     /translation="TLAHEFFKDSWQMNFRELNASDERGIDVVRNQIKQFARTTPLGN
                     ATFKILFLDEADALTTDAQAALRRTMESYAQTCRFILSCNYSSKIIDPIQSRCAIYRF
                     KPLGPDAVMEEVRRIACREGLTITYDAMNAIVYISQGDMRKAINALQGAAILNSTIDE
                     KMVYAITSTARPDEISDLLKSSLNGDFEGAESLLTNLLHQRGIAPNELINQCYRAIIR
                     MEMNRELKVELIDQLGETDFRLSEGANSDIQMEALIARFVLASQRSR"
BASE COUNT         1457 a         1655 c         1449 g         1717 t
ORIGIN      
        1 tgaatcgttg cagaaaaacc tgcgctcttc acatactttt ttatcaggta ttcaacagtt
       61 ccgatactgt gaaggtgaat gacactgcct tcacgcgcat gcctcagtgc gaatggaagc
      121 agtgatactg catcaaaatg gcccataata atccggtcat aggttcccgt cagaaggtcc
      181 ctgcagtctc ccaatgaact gctgatccga tcttcaagac cattctttcg gatattacgg
      241 ttgaggtatc cgaatgcgac aggatttatc tccattgcat gaacagatgc gccactcccg
      301 gcaatgggta tggcaaaata cccgatacca gcaaacatgt cagctattct ttcaggtgta
      361 tcactgttat gcacaatctg tgcaatgcgc attttttcaa ccctgtttcc ctgtgcaaac
      421 atcactttac cgggatcaag aaaataggta tacccgttct cgtggtgctg gacttctcca
      481 gtttcacccc agagaacatg tgttttcggg gtccgcgtca catcctgaag ggattcgatc
      541 cagagcacac cccggggatg acggaagccc acgatctcat ctacttcctc cggacggggt
      601 tttttaccgt ggatgacggc gatatccccg accatgaaaa aaccttttcc ctggtagcgg
      661 atttttgggg agagttcccg gtcacacgtg tcttcatctc tgaccggtac ccaggcaata
      721 tccccctcag tgtacggcct ccgcgtgtgg tcgatccagg tctcgcccgc gatctctctg
      781 agtttgtctt ttgatacagc cctgaccctc attttttacc cgaccacgat gagttgatcc
      841 ccatcacgaa gggtaaggac atgttgtccg gttctggcat cgagccactt cagtctcctg
      901 acctcttttg tctggcaggt gagggggtcc aggataccca tgatgcggtc atcagcaaat
      961 actaccaatg ccgattctgc gttccgcata ttaccgataa ggtggtcaat gtcgtcctcg
     1021 cgtacagatc ggggagtgcc atcagtgaga tcaatcactt tcaggtgatt tgattcaacg
     1081 cgtaccactt ctacataccg cccgtggctt actacaacat cctgtttctg gaacctcggg
     1141 agtctcacgg aaaaggtaat acgatacagt tgcctcccgt ttttctcgcc caccagtttt
     1201 ggatgtgtcg aaaccctgcc ccccaactga gcaattattc cctgagaaat gagtgtcccg
     1261 atatgctggg agccgacagt gatatcaagc ccttcccgtg tttcacccat gtctgcgatg
     1321 aatgaaagac gctccccccc cgcctggagg gaatcttcga cctgctgggc aattccagcc
     1381 gacatctgaa gttcgaaggt actgggaggc cgcccgtcag cacgaacctg aacaatcccc
     1441 tcgtagtaac tcccgcttat ccggttacag cgatcgcact gctccttatg ccagatcagt
     1501 tcaacactgc acgcgtcttc aaccggcgat ccatagagta accccttaag tacaattgtc
     1561 gcagttgaac ggttcagggt gttgtccacg acagttactg acatcgatgt tttcctgaca
     1621 tcgggatgaa agtgaacagc agtctttgca agctcaggtc ccagcagggt acggtcacat
     1681 tcagtatcgg tccatgtgtt cacctgtttt atcgcactgc aacttgggca ctgggtactc
     1741 ttcacacgcc ggtcgcatgc aaaccatgga gtattcccga tcctgcagcg attgcagagc
     1801 ccttcctggt cagtaggttg tccacatttt gggcagaaac gatcctggat gctcataacg
     1861 agtagacctg tgcatggggt atatccccga tcattataca gcccgaaagc gtgatgagcc
     1921 ccatatcaat cgcaatgctc acggaacgtt tgcccatcag gttgatatta cccgctcttt
     1981 tcaatgcatc tctcaccatc tcttcacttg cggggcaatt gccataaaac gcttcagaga
     2041 ttttaacaga aaggtttccg ttactgatgg ttgtgttcag taattcgcga tcgcacacgg
     2101 ccacgatatc acccacaccg ggtgaactat gaaccttcaa aaacattgat tacttattag
     2161 ccgggtggaa taaaaaaggt gagttggagc atggttacag cgcaatcctc acaccataga
     2221 ccctgtcaat cgtttcagcg tggatccgga agcagtcatc ttcggtaatc aggccatcct
     2281 ttagcaactg gttcgtatac cggggtactg attttggacc catcaccgcc cccggccttg
     2341 aattttcatc cataaaatca ctctccatgg taaatacccg ccgctcacgg atcatctgcg
     2401 gaatagcctc atgtttagcg atgagggagg gatggagcgg tgtgtccgga gaaccgtaat
     2461 gttttatcac gcgttcgacc gggaaccctg cattacgtgc catttctgct acatcgttgc
     2521 atgggccgct ttccgcatgg atctgcagcg cgcatttgca ttcagcagcc ctgtccaaag
     2581 catgtgcaag aatttcgttt gaccctctcc atacctccgg gctcacctca tagtgaggtc
     2641 tgccgctttt gagagcgatt gcttttccct cgcgcacata cccggctgcc agatctatgc
     2701 cggctttcat tacctcaatt gcttctgtca atggcatgcg ttcagacaac ctgttgatct
     2761 cagccgggtg aatccccaga accggataga caactacacc ggtttcttca atcagcctgg
     2821 taaccctgag agtctcatca aagactgctg agaaatcagt acctttagaa ggatggacgg
     2881 acagcgacca cgagggtttt gatacaagaa aaaaatgtgt gccccccgcc cgtaaaaagt
     2941 cttttgctgc ctctactccc cgtccattta tcggatcaat atgtatatga tcgtcagtga
     3001 cgggaaattt cggtgattgc atagcactcc acaacctgca tcaggggata tccgttttca
     3061 ggagcgagac gtgcccggca gtaggtaaac ccgacacggg ttacgatttc cacatcgaac
     3121 atcagcccgg acaattcgct atacccgaat atattttgcg tcatcagggt gcggtcaatc
     3181 ctgaccgtga tccgttcaac aaatggctgg aggatcactg ccttttcaat agcactttcg
     3241 atagatccgg cagacgtttt agaaatgggt gtgccgaccc actggtggta aagagctccc
     3301 agttttatcc ctgcttcaaa aattgccctg tcccgttcag ttatcatagt ccctcatcct
     3361 cagattaatt gcaaaatacc cagtttgggt tccattgctt ctccggatcg gacgagcata
     3421 tcgatccccg cctcaacttt atcccgggag aatccttttg agactacgag atcgacaacc
     3481 tgatcttttg ctgcccgttt gccatctccc ccgatattac gaattgcatc ttttatcact
     3541 ttgatgatgt cccgtttttc cttggaaatg cccgttgcta tcttgtcaat atcaaagctt
     3601 cctgtcctgg catcatacgc aatctggcgc aggcaggcgt caactatatg gattacgcgc
     3661 tccgcatcgt gttgttcgat agtatttgac aatcgtatac gcgcacttgc ttctgcaagc
     3721 ctgacaagtg cctcaagctg ccgggcagta accggaaccg gcttattttg ctcggcgatc
     3781 cctctgagct tgagataata actgacaagt gcatcttttg cctcagttga caggatggga
     3841 aaacacgatc gcttcgagta tgcgacatat ttacgaaaga gcgggggatc gatatccggc
     3901 atcaccggtt tgagctgctg ctggatgtat tcatcagtaa tacccgggat gggggtcttc
     3961 ttatgctggg ctatcaattc gccgattcca tgggatttga ggatatgttc ggcaatggcg
     4021 agatcgagct tctgttcggg ctggtcagtc ataatgaaaa tgagatcaaa ccgggaaagc
     4081 agtgaggggg gcatattaat ctggtcagaa atgtctccat acatatcgaa tctgccatat
     4141 ttcggatttg ccgcccccag cagggcgcag cgtgatctga gagttgcggt gatcccggct
     4201 tttgccacgc tgatggattg ttgttccatg gcttcatgca gggcagagcg gtcctctttt
     4261 tgcattttgt ccatttcatc aactgcgcaa acacccatat cagcgaggac aagagcaccg
     4321 gcttccagcg tccagcgccc ttcaccaaat tcgtctttga ctgcagttgc agtcagacct
     4381 gcagaagttg aagactggcc gctggtataa attgcccgtg gcgaaagttt gaccacataa
     4441 cgaagcagct ggctttttgc gatacccggg tcaccgataa gcagcacatg gatatcaccg
     4501 cggaggtggc tgccgtccgg catctccttt gcaatgccgc cgaaaagctg gagagcgatt
     4561 gcttctttga cattttcgct gccataaatg gtcggtgcta tagaatgggt tatcttgcca
     4621 tagatcaggg gatcgctgct cagcgctttg atctcgtcct cgtccttttc attaatatcc
     4681 acttcctcga actctttctc agcgatctcg atagagttgc attccagaaa aatatcaaaa
     4741 accgtgcttt tttctccttt gactattctc tgcatagagc gcagaatgcc gtttattaca
     4801 acccggtctc ccggtgccac catgccggaa agatcatcgg ttacatccac atccagggtc
     4861 tgaggttgtt caccaccccg gagtccctcg ggagactcct gtatccgtag tttttgggaa
     4921 tcgacaaatt tcgatcgctt gggtaacaat tcaagttttt tgaaggaaca cccgtcagtt
     4981 gcgcacccct caggttcaat gaatttcccg tatttttgtt tttttatcgt aaaatgacct
     5041 gcgggacatt tgaataccgc ttcaatgaca cgcggtctga cttctgtggt cttgcgcagg
     5101 atcccttcaa tggaaagaaa tttattgatg tcatcggacc tgatattccg gatacccact
     5161 ttttttggga ggttggtaaa cctgatgttt attcccttgg gttcttttcc atcttttatc
     5221 ctgatcagct ggctgttttt aatcgcatcc cagacatctt ccaaaacttt gcccggattt
     5281 tcgaggagtt cgtcagcaag tgcgattccc gctttaccaa acctttcgac atcccggtaa
     5341 tcaatataaa gggaacgttt atggggatat tcgcgggaaa tctcaccaat ctctttttta
     5401 tatttacttt ttagtaacct gctccagttt tctgtcttgt cagtatcgtg cagctcaggg
     5461 tttttatcca tatcgcgtcc tcacctgctc ctctgcgagg cgagtacgaa cctggcaatc
     5521 agggcctcca tctggatatc actgtttgcc ccttcagaga gcctgaagtc agtttcacca
     5581 agttgatcga tgagctctac tttcagttcc ctgttcattt ccatcctgat gatcgcccgg
     5641 taacactggt tgatcagctc attgggtgcg ataccgcgct gatgaagaag atttgtaaga
     5701 agggattcgg caccttcaaa gtccccgttc agtgaggatt tcagaagatc actaatctca
     5761 tccggccggg cagtggatgt gatcgcgtac accatctttt catcgatagt ggaattcagg
     5821 attgcagcgc cctgaagcgc attgatcgct ttgcgcatat caccctggga aatatacacg
     5881 attgcattca ttgcatcata ggttatggta agaccctcgc gacaagcaat ccgcctgact
     5941 tcttccatga ccgcatccgg ccctaatggt ttaaaccggt aaattgcaca ccggctttgt
     6001 atgggatcaa taattttgga ggaataattg caggaaagga taaacctgca ggtctgtgca
     6061 tagctctcca ttgtccttcg gagggctgcc tgggcatcgg tagtgagtgc atcagcctca
     6121 tctaaaaaga ggattttaaa cgttgcgttt cccaggggag tcgtacgtgc gaattgcttg
     6181 atctggttac ggacaacgtc aattccccgt tcatccgagg cgttcagttc acggaaattc
     6241 atctgccatg aatccttaaa aaattcatgt gccagtgt
//
LOCUS       DASRLV010000097         9879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_434696,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000097 DASRLV010000000
VERSION     DASRLV010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 9879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9879
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434696"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VFC43_03880"
     CDS             <1..387
                     /locus_tag="VFC43_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HZK30358.1"
                     /translation="LKAIVLLGISSGVIWLLDILACVSVVMMFQQQIPFAIIVLAIVI
                     GNLVKAVPLTPGGVGTYEISLAITFGLAGVDPAVATLIAIIDHLIKNLVTLAGGIVSI
                     YYFGDWVIPSIRTALDSKFGGDRPDR"
     gene            350..2377
                     /locus_tag="VFC43_03885"
     CDS             350..2377
                     /locus_tag="VFC43_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HZK30359.1"
                     /translation="MIQNLGEIVLTVEQQIFSVITWLVVLTLLQLSFYPSLKKTFDLF
                     AFPVSFSASLLAFTILSWYCGLLRIPVQIALLPFLLLFAYHLYHHHYSLDELMREWRW
                     EVLFLICFFLMLDVRFVNPTISYAEKFMDHAFLASVMRNPVVPPLDPWFAGGSLNVYY
                     YLGYWMFGCLGIVTGVPSNIAFNLALPTIFSLAAINLYAIGTLLMEKFRWLPLIVFFI
                     PNPSFFYQLAQGKALNTVLWDSTRTITNTINEYPFFSFVWGDVHAHVVSLFNQVFLIF
                     LLLYAYKRWDSLENRAKWLISIVCALSLGSMPLFNTWDVLIYAPITLVFVALIIRKGR
                     KKVSDISSLWQLGTVPPFAILFYLPFYLQLKTNTGGISIVHTPSNPFEFLLVNGLFIA
                     IFLAFLWRDIVRRPYLLLAVIPFAFTGYIAAAIAIIPLVYFIERKHHDLSDVLASAGL
                     TILIFCEIFYLKDNMGDTYFRMNTVFKCYLPVWILLGIAASSMIGKWLSEKKWIPVVS
                     ARNKAIMTASVVGILFVLPFVIPFNLNYGTGTLDGLAYLKDSHAGDAGAVTYLRTLQG
                     DDRIVEAEGGDYTYYSRVSSFTGIPAIIGMPFHEYMWRGDDTGWFSTRLADVQSIYED
                     PEKTVELMKKYNATLLYIGDSERERYRVNSSANGLEIIYSADGAEIYRLSG"
     gene            2534..2902
                     /gene="rpl7ae"
                     /locus_tag="VFC43_03890"
     CDS             2534..2902
                     /gene="rpl7ae"
                     /locus_tag="VFC43_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7Ae"
                     /protein_id="HZK30360.1"
                     /translation="MAKGYVKTQAPEELQNKALEALEVARDTGKIKKGSNEATKAIER
                     SIAQLVLIGADVEPEEIVMHLGPLCDEKKVPYIFINKQNDIGAASGLDVGSAAAAIVK
                     PGKAKETIEDLTKQLTALKA"
     gene            2913..3122
                     /locus_tag="VFC43_03895"
     CDS             2913..3122
                     /locus_tag="VFC43_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S28e"
                     /protein_id="HZK30361.1"
                     /translation="MADDATPAEVIEVVGSTGMHGEAMQVKCRILDGNNKGRIITRNT
                     VGPIREGDIVMLLETEREAKKMSRR"
     gene            3128..3319
                     /locus_tag="VFC43_03900"
     CDS             3128..3319
                     /locus_tag="VFC43_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24e"
                     /protein_id="HZK30362.1"
                     /translation="MVEKHPCSFCGIQLEPGTGKMYVRKDGTIFYFCSTKCQNNYKLG
                     RVPRRVPWTNAGKKAANKE"
     gene            3328..3780
                     /gene="ndk"
                     /locus_tag="VFC43_03905"
     CDS             3328..3780
                     /gene="ndk"
                     /locus_tag="VFC43_03905"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HZK30363.1"
                     /translation="MDRTFVMVKPDGVQRGLVGKIVSRFEAKGLKLVAARFEILPETR
                     VTEQYREHLAKPFFPSLKQYIMGGPCFLMVWEGRSVVSIARKVIGATNPQEAEPGTIR
                     GDFGIDIGRNVIHGSDSAESASREIAIHFRHNELVEYKRIDESVIYEY"
     gene            3858..4508
                     /locus_tag="VFC43_03910"
     CDS             3858..4508
                     /locus_tag="VFC43_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HZK30364.1"
                     /translation="MAGEVLSFALLSLSSIIIIVNPLGATLMYVSLTTTLDRPAREVI
                     AKDACRFALLILLFVAVLGAWILQLFGIGLEAFRIAGGILLFGIGMEMVYAKTSRTNL
                     TATEKYESREPDDISIMPLAIPMIAGPGAITTTIVLMNEAVVITPFAIGIVFFSIGVS
                     IGITYFMMRHSDYIMSKIGQREYRAINRLMGMLLIAIAVQFVINGIKIAFPLFAGG"
     gene            4511..4711
                     /locus_tag="VFC43_03915"
     CDS             4511..4711
                     /locus_tag="VFC43_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30365.1"
                     /translation="MEDWDLTSIGLIVIVCSITVAGTIIGAMLLGFPVDRAPYLLIIT
                     LVLIVMGAISVFYENTNINFRK"
     gene            4680..5534
                     /locus_tag="VFC43_03920"
     CDS             4680..5534
                     /locus_tag="VFC43_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HZK30366.1"
                     /translation="MRTLTSISENDAERGDGGHIIRVCSAQIMSAWEDPEKTLTKAEI
                     FIRHAADCGAKLICFPEQFATGWDPKPHANIQNIRGSITTSLQAYAKEYRIGIIGSIR
                     EEQDPLPKNTAIVINRHGRILAKYAKVHLFSPGHEHEGNSAGTDLGIFTFDSITCGIA
                     ICYDLRFPELFRIYAQKGVHAVFVPSAWPQSRIRHWELFIRARAAENQMYVFGVNTTG
                     KTPIDQYSGASMTADPHGTIISRANEAEQLLFSDIDPDEVERMRNAFPVEKDRKDKLY
                     HFLLTKTK"
     gene            5927..7132
                     /gene="thrC"
                     /locus_tag="VFC43_03925"
     CDS             5927..7132
                     /gene="thrC"
                     /locus_tag="VFC43_03925"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZK30367.1"
                     /translation="MYHLVCVNCGATYPADEILYNCKKCGHLLAVKYPLDEINVTKAM
                     WESRPLSVWRYRELLPVRIDPVTLHEGGTPLYHLKRLGEEMGLKHLYAKHEGMNPSGS
                     FKDRGMTVGVSMALQLGKKSVACASTGNTSASLAVYAAKAGIPAVVLLPAGKVAVGKV
                     AQALMHGAKVISIRGNFDRALEMVHDLCLSHGLYLLNSINPYRLEGQKTIGFEALDQL
                     GVVPDRFVLPVGNAGNISAVYKGFLELQELGFLDRIPMMTGIQAQGSSPIVRAIRENL
                     PEVIPEQNPETVATAIRIGAPVNAEKALTAIRKTGGLAESVTDAEILQMQRDLARKEG
                     IGVEPASAASVAGIRKLVENGMIDRNERIVCVVTGHLLKDPDTVIKQCEPPTEINADL
                     PSLLSALHL"
     gene            7069..7710
                     /locus_tag="VFC43_03930"
     CDS             7069..7710
                     /locus_tag="VFC43_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5803 family protein"
                     /protein_id="HZK30368.1"
                     /translation="MRTPDRDQRRSAIAALCTALIIVLAAIPSAGALSADYLIFPNGT
                     LYRASIEIVDSSRYEFVDTGMLGEPTPLTVGEVQLSGNCSPCLFNKTGPSFTSNVMAI
                     TFEQGNYTVSYVSPLRDNHLQASFRNPYHVNISLPEEFDVQNPLLAGISPGANVTRYT
                     DNSTLIQWNKTLSVDLRFYDQGRETLLYFFLQFMGIIAVVMLLPFLITLRKKQ"
     gene            complement(7797..8798)
                     /gene="ruvB"
                     /locus_tag="VFC43_03935"
     CDS             complement(7797..8798)
                     /gene="ruvB"
                     /locus_tag="VFC43_03935"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HZK30369.1"
                     /translation="MTERTVSPEPLIEEADDLTIRPARLDEFVGQAQVKETLKIAIEA
                     AQKRNEPLDHLLFSGPPGLGKTTLAHIIAHEMGADIRATTGPVLEKPGDIAALLTPLK
                     RGDILFIDEIHRINPVVEEMLYPAMEDFFIDVMIGEGPSARSIKLNLEHFTLIGATTK
                     QGLLGAPFRDRFGIISRLDLYSSVELVKIVTRSASILKIPATADGADEIAKRSRGTPR
                     IVNRLLRRVRDYASVKGDGTITREITARALRMMQIDELGLDELDRRILSVIAKDFDGG
                     PVGAKTIAISVGEEVRTIEDVYEPYLIQIGFLKRTPQGREVTPAAKKHLNNPQKTLL"
     gene            complement(8803..9399)
                     /gene="ruvA"
                     /locus_tag="VFC43_03940"
     CDS             complement(8803..9399)
                     /gene="ruvA"
                     /locus_tag="VFC43_03940"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HZK30370.1"
                     /translation="MIAHIYGKPVLAGDKWAVIDVGGIGYQVQVTQPALQQLAHTTEP
                     VKLFTYMAVREDAITLYGFMHQSELEMFRILIGVSGIGPQIALNLLSQIGIEDFVMAI
                     LNEDEKALTRISGIGTKSAKRLILELRDKMRKVSETMVAGDIGMASPAVHDAVSALVS
                     LGFSEKESRDAVNDTARVMKTPTVQDLIKAALVKMKER"
     gene            complement(9404..>9879)
                     /gene="ruvC"
                     /locus_tag="VFC43_03945"
     CDS             complement(9404..>9879)
                     /gene="ruvC"
                     /locus_tag="VFC43_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HZK30371.1"
                     /translation="MIVIGIDPGLARLGYGVIEVTKGEKRVLCYGCIETSGKDLRASE
                     RLLKIYTAVEALFEKYQPSHVSLEKLFFAKNISSAMGVSEVRGIILLAAEQRTIPIVE
                     YTPNQVKQAITGSGRADKRQMQEMIKRLLRLDEIPTPDDAADALSIALCHIHILR"
BASE COUNT         2470 a         2382 c         2350 g         2677 t
ORIGIN      
        1 ctcaaggcaa tcgtattgct ggggatttct tcgggggtaa tctggctcct tgacatactg
       61 gcctgtgttt ccgtagtaat gatgtttcag cagcagattc catttgccat tattgtcctt
      121 gcaattgtaa ttggtaatct cgtgaaggct gttcccctca caccaggggg tgtaggaaca
      181 tatgagattt ctctcgcaat aaccttcgga cttgcagggg ttgatccagc agttgctacg
      241 ctgatcgcca ttatcgacca tctgattaaa aacctcgtca ccctcgcggg gggtattgtc
      301 tcgatctatt acttcgggga ctgggttatc cccagcatcc ggactgccct tgattcaaaa
      361 tttgggggag atcgtcctga ccgttgagca gcagatattt tccgtgataa cctggctcgt
      421 ggttcttacc cttctccagc tttcattcta cccgtcgcta aaaaaaacat ttgatttatt
      481 tgcatttccg gtctcatttt ccgcatcact tcttgcattc accattcttt cctggtactg
      541 cggacttctc cggatacctg tacagattgc ccttctgcca tttcttttgc tttttgctta
      601 ccatctttat caccaccact attccctgga tgagctcatg agagagtggc gctgggaagt
      661 gttattcctc atctgttttt tcctcatgct tgacgtgcgg tttgttaatc ccaccatatc
      721 atatgcagag aaattcatgg accacgcgtt tcttgcatca gtgatgagaa atcctgtagt
      781 accaccactt gacccatggt ttgccggagg ctccctgaat gtttattatt accttggata
      841 ctggatgttc gggtgtctcg gtattgtcac cggggtacca tcgaatattg cattcaatct
      901 cgctctgcca acaatcttca gtcttgctgc gattaacctg tatgcgatag ggaccctcct
      961 gatggaaaaa ttccggtggt tacccctcat tgtttttttt atcccaaatc catcattttt
     1021 ttatcagctt gcacagggaa aagcattgaa tacggtatta tgggatagta cacggacgat
     1081 taccaatacc attaacgaat atcccttttt ctcattcgtg tggggagatg ttcatgccca
     1141 tgtggtctcc ctgtttaacc aggtattttt gatcttcctg ctcctctatg catacaaacg
     1201 gtgggactcc ctcgaaaacc gggcgaaatg gctcatcagt atcgtatgtg cactcagtct
     1261 tggctccatg ccgttgttca atacctggga cgttctgatt tacgcaccca ttaccctcgt
     1321 gttcgtggca cttatcatcc ggaagggcag gaaaaaagta tccgatattt cttccttgtg
     1381 gcaattaggt actgtcccac catttgccat cctgttttat ctgccattct atctccagct
     1441 gaaaacgaac accggtggca tctcgatcgt tcataccccc tcgaatccct ttgagttcct
     1501 tttggttaac ggactattca ttgccatttt tttggcattt ctctggcgcg atatcgtacg
     1561 gcgaccgtat cttctccttg ccgtgattcc ttttgcattt actggttata tcgcagctgc
     1621 aattgcgata atcccgcttg tttatttcat cgaacggaaa caccatgatt tgtcggatgt
     1681 ccttgcaagt gccggactga caattttgat cttctgtgag atattctatc tcaaggataa
     1741 catgggcgat acctacttcc ggatgaatac ggttttcaag tgttatcttc ctgtatggat
     1801 cctgcttgga attgcagcat cctctatgat cgggaaatgg ctgtcagaaa aaaagtggat
     1861 accggttgtt tcagcccgga ataaggccat catgacagcc agtgtagtcg gcattctctt
     1921 cgtactaccg tttgtcatcc cgttcaatct gaattatgga acaggaactc tcgacgggct
     1981 tgcgtatctc aaagactcac atgccgggga tgccggggca gtgacctatc tgcggacact
     2041 gcagggagat gaccggattg ttgaagctga ggggggggac tatacatatt actcgcgggt
     2101 ttcatcattc accgggatcc ccgcaattat tggaatgcct tttcatgagt acatgtggcg
     2161 gggtgatgat acaggctggt ttagcacgcg actggctgat gtacaatcaa tctatgaaga
     2221 cccggaaaag actgttgagc tcatgaaaaa gtacaatgcc actcttctct acattgggga
     2281 tagtgagcga gagcgctata gagtaaatag ttcagcaaac ggtcttgaga taatatattc
     2341 tgccgacggt gcagaaatat accggttgtc aggataattg gcaccagata ccacgtgaaa
     2401 aaacgaatgg tttttttagt tcccgttaag tccttcgcaa acctttattt ccgctcctga
     2461 tgaaatgata tggctacatc attgctgact gatgagtaat gaaggagcat tctactcctg
     2521 aatgaggtga gttatggcaa aaggttacgt aaaaacgcag gctcccgaag agcttcagaa
     2581 caaggcgctt gaagcccttg aagttgcacg agatacagga aagatcaaga aaggatccaa
     2641 cgaagccaca aaagccattg agcggagcat agctcagctt gtgcttatcg gtgctgatgt
     2701 tgaacctgaa gagattgtga tgcacctcgg accactctgc gatgagaaga aagtacctta
     2761 catcttcatc aacaagcaga acgatattgg cgccgcaagc ggtcttgatg tcggctcagc
     2821 tgctgctgca attgtaaaac ctggcaaggc aaaagaaacg attgaagatc ttaccaagca
     2881 gctgaccgcg ctgaaggcgt gaggattcta ccatggcaga cgatgcaacg ccagccgaag
     2941 tgattgaggt tgttggctcg accggcatgc acggtgaggc aatgcaggta aagtgccgta
     3001 ttctcgatgg caataacaag ggacggatca ttacccgtaa cacggttggt cctatccgtg
     3061 aaggagatat tgtaatgctc cttgagaccg aacgcgaagc aaagaaaatg tcgaggcggt
     3121 gaaaggaatg gttgaaaaac atccctgcag cttctgtggc attcaacttg aaccaggtac
     3181 cggtaagatg tatgtcagaa aagacggaac catcttttac ttctgcagca ccaagtgcca
     3241 gaataactac aagcttggca gggttccgcg ccgtgtccca tggaccaacg ccgggaaaaa
     3301 ggctgcaaac aaggagtgag atcgtagatg gaccgcacct ttgttatggt taagcctgat
     3361 ggggttcagc gcggtcttgt gggtaagatt gtttcccggt ttgaagcaaa aggtctgaaa
     3421 ctcgtggcag cacggtttga gatacttccc gaaacacggg tgacagaaca gtaccgggag
     3481 catctcgcaa aaccattttt tccatcactg aaacagtata ttatgggggg tccttgtttt
     3541 ctcatggtct gggaaggcag gagcgtggtc tcgatcgccc gtaaggtaat tggtgcaacc
     3601 aatccacagg aagcggaacc cggtactatt cggggtgatt tcggtattga tatcggccgg
     3661 aatgtcattc atggttccga ttcggcggaa agtgcctccc gcgagattgc aatccacttc
     3721 aggcacaatg aactggttga atataagcgg atcgatgaat ctgttattta cgaatactaa
     3781 aattcttctc tttttgtttt tcctgccgtt aaaggttacc aaaatgtcta ttactctttg
     3841 acactgcact atgcttaatg gcaggtgagg tccttagttt tgcgcttttg tccctatcat
     3901 ctatcataat tattgtcaat ccactcggtg caacccttat gtacgtctcg cttacgacga
     3961 cgttggaccg ccctgcacgt gaggttattg caaaagacgc ctgccgtttt gcccttttaa
     4021 tcctgctctt tgtcgcggtg cttggtgcgt ggatcctcca gctcttcggc attggtcttg
     4081 aggcattccg cattgccggt ggaattttac tgtttggtat aggaatggaa atggtgtatg
     4141 caaaaacatc acggacaaac cttacagcaa ctgagaaata cgagtcgcgg gaaccagatg
     4201 atatctcaat aatgcccctt gccattccca tgatcgcagg accgggtgca attaccacca
     4261 caattgtgct gatgaacgag gcagttgtta tcacaccatt tgccataggg attgtatttt
     4321 tctcgattgg ggtatccatc gggataacgt attttatgat gcgccattca gactatatca
     4381 tgagcaagat aggacagcgg gaataccggg cgatcaatcg cctgatggga atgttactca
     4441 ttgccatcgc ggtgcagttt gtaataaacg gcatcaagat agcatttccg ctttttgccg
     4501 ggggataaca atggaggact gggatcttac atcaattgga ttaatcgtga tcgtgtgcag
     4561 tattactgtt gccggcacaa ttatcggagc aatgctcctt ggttttccgg tcgatcgtgc
     4621 accgtatctt ctgatcataa ccttagtact catcgtcatg ggtgcaattt cagtctttta
     4681 tgagaacact aacatcaatt tccgaaaatg atgccgaaag aggggatgga gggcatatta
     4741 tcagagtttg cagtgcacaa ataatgagtg cctgggaaga tcccgaaaaa acactcacaa
     4801 aagcggaaat attcatccgg catgcagctg attgtggtgc aaaactgata tgttttcctg
     4861 aacagtttgc aacaggctgg gatccgaaac cccatgctaa tattcagaat atccgtggga
     4921 gtatcactac atcccttcag gcatatgcga aagaatacag gattggtatc atcggatcaa
     4981 tacgggaaga acaggaccct ctcccgaaaa acactgccat tgttatcaac aggcatggga
     5041 gaatcctagc aaaatatgct aaggtgcact tgttctcccc gggccatgaa catgaaggaa
     5101 actcggcggg aacggatttg gggatattta cttttgattc aataacctgt ggtattgcaa
     5161 tctgttatga tctccggttt ccagaacttt tcaggattta tgcacaaaag ggtgtccatg
     5221 cagtttttgt gccatcagcc tggccacaga gccgtatccg gcactgggag ctgtttataa
     5281 gggcacgagc tgcagaaaac cagatgtatg tttttggtgt aaatacgaca ggaaagactc
     5341 caatagatca atactctggc gcatcgatga ctgcagatcc ccacgggacg ataatcagtc
     5401 gggctaatga agcagaacaa ctcctcttct cagatattga tccagatgaa gtggagagaa
     5461 tgcggaacgc tttccccgtg gaaaaagacc gtaaagataa actctaccac tttcttctca
     5521 cgaagacaaa ataacacgaa aaaagatctc aggacttctt agtggaatga taataatcag
     5581 ctctgcagaa atgttcaaat taaaaacctg aacactcacg gatgtttcac ccctgccgct
     5641 tcggctccca aaaaatgcga taacgtcctg aacggtaata cgtcattatc gttccaagcg
     5701 aaggggcggt aaggggtcac acccactttc tccttaatca tcattcctgt gctttttatt
     5761 tcaaaccctg ataaaaggaa ttaccatgaa agacccgggg ttattcatca ggtattcttg
     5821 agtcttcgaa taatgtgtct ttctaaagag ccaaaaaatc aatacacgga cctttcatca
     5881 tatgcgacaa cacccctata tgtaattaaa agaccaatac tgtaccatgt accatctcgt
     5941 atgcgtcaac tgtggagcca cgtatcccgc tgacgagata ctatataatt gtaaaaaatg
     6001 cggtcacctt cttgcggtga agtacccgct ggatgaaata aacgttacaa aagcgatgtg
     6061 ggagagtcgc ccgctttcgg tatggcggta ccgcgaactg ctcccggtaa ggatcgatcc
     6121 ggtcaccctg cacgaaggtg gcaccccgct ctatcattta aaaaggctcg gagaagagat
     6181 gggcctgaaa cacctctacg ccaagcacga gggcatgaac ccctcaggtt cattcaagga
     6241 ccgtgggatg accgtcggtg tctcgatggc acttcagctc gggaaaaaga gtgttgcctg
     6301 tgcaagcacc ggaaatacat ctgcaagtct tgcagtctat gcagcgaagg cagggattcc
     6361 cgcagttgtc ctgcttcctg ccggaaaagt cgcggttggc aaggtagccc aagccctgat
     6421 gcatggtgca aaggttatct caatccgcgg gaattttgat cgtgcccttg agatggtgca
     6481 cgatctctgt ttatcgcatg ggctttattt gttaaattcc atcaatccct atcgcctgga
     6541 aggccagaag accataggtt ttgaagcact cgaccagctt ggtgtagtgc cggaccggtt
     6601 tgttctcccg gtaggaaatg caggaaatat atcagcagta tacaaaggat ttctggagct
     6661 gcaggaatta ggtttccttg accgtatacc aatgatgaca ggcatccagg cccaggggtc
     6721 aagcccgatc gtgcgggcaa tccgtgagaa tcttccggaa gtcattccag aacagaatcc
     6781 cgagaccgtt gcaactgcta tacggatcgg cgcacccgtc aatgcagaaa aggccctcac
     6841 ggctatccgg aagaccggcg gacttgccga gtccgtcacc gatgcagaga tcctgcagat
     6901 gcagcgggat ctcgcccgaa aagaaggaat aggtgttgaa ccggcttctg ccgcttcagt
     6961 tgctggtata cgaaaactgg tggaaaatgg tatgattgac cggaatgagc ggattgtctg
     7021 cgtcgttacc gggcatctgc tcaaggaccc ggatacggtg ataaaacaat gcgaaccccc
     7081 gacagagatc aacgcagatc tgccatcgct gctctctgca ctgcacttat aatcgtgctg
     7141 gctgctatcc cttctgcggg agcactctcg gcagattatc tgattttccc caacgggact
     7201 ttgtacaggg catcgataga gatcgtggac tcgtctcggt atgagtttgt cgataccggt
     7261 atgctcggtg agcccacacc tctcacggtt ggagaagtac agctttcagg gaattgttct
     7321 ccctgcctgt tcaacaagac cggtccttcg tttacgagta atgttatggc cataacattt
     7381 gaacaaggaa attataccgt gtcgtatgtc tcacctcttc gggataatca tcttcaggca
     7441 tcgttcagga atccctatca tgtcaatatt tccctcccgg aggaattcga tgtgcaaaac
     7501 ccgttgcttg ccggaatcag ccctggtgcc aatgtcacac gatatacgga taattcaaca
     7561 ctcatccaat ggaataaaac cttgtcagta gatctcaggt tctatgacca gggtcgcgag
     7621 acactcctgt attttttcct gcaattcatg gggatcatcg ctgtcgtgat gctgttaccg
     7681 ttcctgatca cattaagaaa aaaacagtga taaaaaactc cttggaccag gatgtaaaga
     7741 gcagaggggg gataatccct tattatccag aatttaaaac tattcgtaac cctgccctac
     7801 aaaagcgtct tctgcgggtt attaagatgc ttttttgcag cgggtgtgac ttcccttccc
     7861 tgaggtgttc gttttaaaaa gccgatctgg atcaggtaag gttcatatac atcttcaatg
     7921 gtccgaacct cttccccgac cgagatcgca atggtctttg cacctacagg acccccgtca
     7981 aaatctttgg caataactga gagaatgcgc ctgtcgagct cgtcaagtcc gagttcatcg
     8041 atctgcatca tccggagcgc tcttgcggta atctcgcggg tgattgtgcc atcccctttg
     8101 acacttgcat aatcccgaac gcgcctgagc agacggttga ctatccttgg tgttcccctg
     8161 ctcctttttg ctatctcatc agcgccatct gcagttgctg gaattttcag gatggatgcg
     8221 ctccgtgtga caatttttac gagttcgact gaagaataca aatcgagccg ggatatgata
     8281 ccgaaccggt cccggaacgg ggcacctaag aggccctgtt ttgttgttgc cccgataagg
     8341 gtaaaatgtt caaggttcag ctttatcgag cgtgcactcg gcccttcacc gatcatcaca
     8401 tcaataaaga aatcctccat tgccggatac agcatctcct caacgaccgg gtttatccgg
     8461 tgaatttcgt cgataaacaa aatatcaccc cgttttaaag gggtgagaag ggcagcaata
     8521 tcccccggtt tttcaagcac aggaccggtc gtagccctga tatcagcacc catttcatga
     8581 gctatgatat gcgcaagtgt ggtcttgccg aggcccggcg gcccggaaaa caggagatgg
     8641 tccaggggtt cattgcgttt ctgtgctgct tcgatcgcga tcttcaatgt ttctttgacc
     8701 tgtgcctgcc cgacaaattc atccagccgt gccggcctga tggtaagatc gtctgcttcc
     8761 tcaataagtg gctcgggtga gacagttcgc tcggtcatgg catcagcgtt ccttcatttt
     8821 aacgagtgcg gctttgatga gatcctgcac cgtcggggtt ttcatcaccc gggctgtatc
     8881 atttactgca tctcttgatt ctttttctga aaaaccgagc gatacaagtg cacttactgc
     8941 gtcgtgaaca gccgggcttg ccatgccgat atcccccgca accatcgtct cgctgacttt
     9001 cctcatcttg tccctgagtt cgaggatcag ccgtttcgca ctctttgtac caatcccgga
     9061 gatcctggta agcgcttttt cgtcctcatt caggatagcc atgacgaaat cttcgattcc
     9121 gatctgggaa agaaggttca gggcaatctg gggaccaatt ccgcttaccc cgatcaggat
     9181 cctgaacatt tccagttcac tctggtgcat gaacccgtag agcgttatcg catcctcacg
     9241 gaccgccata tacgtgaaga gtttaactgg ttctgtcgtg tgtgccagct gctggagtgc
     9301 gggctgggtt acctgcacct ggtacccgat ccccccgaca tcgattaccg cccacttgtc
     9361 acccgccagg accggtttac catagatatg tgcgatcatt gtactatctc aggatatgga
     9421 tatgacataa ggcaatcgaa agggcatcag ccgcatcatc aggagttgga atttcatcta
     9481 accggagaag tcttttaatc atctcctgca tctggcgttt atctgcacgc ccggatccgg
     9541 tgatggcctg ctttacctgg ttcggggtgt attccacgat tgggatagtc ctctgttcag
     9601 cagcgagaag tatgatcccg cgtacttcac ttacgcccat ggccgaagag atgttctttg
     9661 cgaagaataa tttttcaaga gaaacatgag aaggctggta tttttcaaaa agtgcttcga
     9721 ctgcagtata tatttttaat aacctctctg aagcgcggag atccttgcct gatgtttcta
     9781 tacatccata acagagcacc cgtttttccc ctttagtaac ttcaattaca ccgtacccca
     9841 gtcgggcaag tccggggtcg atcccgataa caatcattc
//
LOCUS       DASRLV010000098        15575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_114764,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000098 DASRLV010000000
VERSION     DASRLV010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 15575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15575
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114764"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..548)
                     /locus_tag="VFC43_03950"
     CDS             complement(24..548)
                     /locus_tag="VFC43_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZK30372.1"
                     /translation="MFARDVMTDTVFFVSPKETIAYARNLMVKHKISRLLVMEEGQLT
                     GILTKKDIAYHIRQGEPAWRRRPIDQIPVEAFATASPVVVAPDTGIKTIAGIFAKKNI
                     SSVPVIEAGKILGIVTKTDLMKSSLVRNLTCPVRDIMEDVATVSRFHSLDHVITVMSE
                     RHDKAQQLCHAVRS"
     gene            complement(554..1495)
                     /locus_tag="VFC43_03955"
     CDS             complement(554..1495)
                     /locus_tag="VFC43_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZK30373.1"
                     /translation="MHQNDRSIKQGDRLLKMQGKLDRGPVEFKSHIVQQEGEIMAIAT
                     TDVISVPPTQSIIGAVEIMTSCGFRRLPVTDAGTKKLRGIITSGDIINFMGGGDKFKL
                     VQVRHGGNLRAAVNESVRTIMTQQTTTLSHESRISDAVTMIVEKKIGGMPIVDDEGVL
                     NGIVTERDVLRVLGADRSTKNVEDIMSSSLRVTAPDCPIAEVARDMIRYHFRRLPVVS
                     DDVLYGMVTATDIMKYLGSCEVFSRLTTGNIDEVMGLPVRTLVAGDLFTTTPMQNINQ
                     AAREMLTRNIGALPVIEGSQLIGLVTEFDLVRAFAKG"
     gene            complement(1598..2347)
                     /locus_tag="VFC43_03960"
     CDS             complement(1598..2347)
                     /locus_tag="VFC43_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZK30374.1"
                     /translation="MKTAQDFMIGIPVLKYNDQITKARQILRDDRFREVYVVDAKKNI
                     LGYIDLTDGLRVTATKSNVTVEGYIKEGPTVSPADSIEQVARVMRDNETDSVAVVDSS
                     RHIIGGVLLSDVFPVITSRHELRGTVASHMTTEVIIASPTDTIQKIYTMIMESGFSAF
                     PVVKKKRIVGLISRRDLIRTRRVQSVIAHHAQTTIEEIMSKDVITISPDEPIGSAAEL
                     LVKHDVSRLPVTDGDRIVGIVDRHDVLRGLA"
     gene            complement(2344..2910)
                     /locus_tag="VFC43_03965"
     CDS             complement(2344..2910)
                     /locus_tag="VFC43_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZK30375.1"
                     /translation="MKKTTNGMRFETQVPLRDVMKNNPTMIGIEANVAMAAKAMCTDE
                     VGSVIILENNKPTGIVTEEDITCKVVAKDLKPSTVQVREIMSTPLITVSAEKTVGDAA
                     HMMVKHKVRRLPVIDAKKKVIGIVTVRDLLTVSNELNELLEDLIEINREEIVEQGMCN
                     RCSQMSDDLKRVDNVMLCTRCREEDNIA"
     gene            3161..3814
                     /locus_tag="VFC43_03970"
     CDS             3161..3814
                     /locus_tag="VFC43_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Era-like GTP-binding protein"
                     /protein_id="HZK30376.1"
                     /translation="MKIFYQTYGTAKKKFSSFLKRFFKKKRTRIGIYGPPNAGKTTLA
                     NRIARDWTGDAVGPVSEIPHETRRARKKEDIIISGANGSTITMDIVDTPGVTTKIDYH
                     EFLEFGMDKDEAIIRAREATEGVAEAMHWLREDIDGVIYMLDSTLDPFMQVNIMMIGI
                     IESRKLPVIIVANKIDLADAVPARIRSAFPQHPVVAISGLEGRNVENLYETMLECFG"
     gene            3821..4216
                     /locus_tag="VFC43_03975"
     CDS             3821..4216
                     /locus_tag="VFC43_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing protein"
                     /protein_id="HZK30377.1"
                     /translation="MPHKCTKCGREYRAGSTEILKGCASCGGKKFLYVKEAEIHRDVL
                     DEKSMDEIADESHEDILEIVEPRKKEQVEMYDRVETIRIVSPGSYELNLEKMAQSDQR
                     IVSVGKEGNYIIDLMSMAKDDKKKKKGKK"
     gene            complement(4226..4963)
                     /locus_tag="VFC43_03980"
     CDS             complement(4226..4963)
                     /locus_tag="VFC43_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30378.1"
                     /translation="MNKTPLETIDDESAIRIRDCFSVRYIQSAALLCRLGYRIEREFA
                     SSGMISEEEHLRHEAYILNSVLSSVAFLESTINELYADAADDAYFFIDEQKGMLLKTI
                     GERWRNEKNFDRAPLLNKYQKILVIADKPPFDEGDPVFSDIRTLIEIRNYLMHYKREW
                     IVVQQGDNPDNNTETHGERLEKILRNKFAPNPLAQQLRPFFPDTCFGHGCAEWAVINS
                     LAFTDDFFKRLDLPPPYEGIKNELETK"
     gene            complement(5082..5366)
                     /locus_tag="VFC43_03985"
     CDS             complement(5082..5366)
                     /locus_tag="VFC43_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30379.1"
                     /translation="MVKKCESCDKFTSEDAAAFCLHCGNRFPEDPCVLLHQIRDSVDE
                     SQEEIRTIRILLRKSQKKTRLGKPLTYVVIAAVVIILIAAFLFFQAGARF"
     gene            complement(5521..7155)
                     /gene="thsA"
                     /locus_tag="VFC43_03990"
     CDS             complement(5521..7155)
                     /gene="thsA"
                     /locus_tag="VFC43_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit alpha"
                     /protein_id="HZK30380.1"
                     /translation="MSQQLGGQPIIILREGTTRNRGQEAQNNNFAAAKAVAAAVRSTL
                     GPKGMDKMLIDGMGDITITNDGVTILKEMDIEHPAAKMMVEVAKTQDDEVGDGTTTAV
                     VIAAELLKNAEDLLSQKVHPTVIAEGYRLATTKALVILDGFSITIKPSDTVMLKKIAE
                     TALTGKNAEVTKDHICDIIVKAIGFVTDPDGKVDISHINVEKKVGGAVDDTNLIEGMA
                     IDKERAHPSMPKSVMNAKVLLLNAALEFKKTEVNATIKISTPGQVQAFLDEEEQMVHT
                     MVDKVVKSGATVLFCQKGIDDVAQHYLAKAGILAVRRVKKSDIENLARATGANIVNSI
                     DTITTKDLGSAGLVEERKVSGDEMIYVSKCKNPKAVSIIVRGGTEHVVDELERAMHDA
                     LMVVSVVIEGKKIVAGGGAPETELSLQLRRYASQEGGRVQLAIEAFASALEVIPRTLA
                     ENAGLDPIDMLVAIRAAHEAGKKNFGLNVYEGKPVDMLKAGVVEPLRVKTQAISSAAE
                     AAVMILRIDDIIASSKAAGPAGGMPPGGMPPGMGDY"
     gene            complement(7334..7708)
                     /locus_tag="VFC43_03995"
     CDS             complement(7334..7708)
                     /locus_tag="VFC43_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HZK30381.1"
                     /translation="MRQENVEFFTDREEEFAMLLIRIGTRKNIAKALVFLANTKETTS
                     HAIERGTDMRQPEVSLAMRYLAEQGWITTRESESESKGRPVKIYTLAKPLQEIMDCIE
                     KEQVHEAENQLALVRKLRDHLK"
     gene            8126..8785
                     /locus_tag="VFC43_04000"
     CDS             8126..8785
                     /locus_tag="VFC43_04000"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZK30382.1"
                     /translation="MAKANIPEKGAIIQRDMETYSISPHIPGGFIEPAMLRKIADVAE
                     KYNARYVKLTGAQRMAIIGIREEDLDKAWAEFTDRSKAIGLTIRSIQICPGTRACKKA
                     KQDSPGLGFALDQEFYGKPAPAKFKMGVSGCQNCCSDSWLKDIGFFGTDEGFTAVAGG
                     KGGGTAKPGRELAKGLTADQAVALARRVIAFYRENGKFPERLGATIDRVGFDTFVKAV
                     S"
     gene            complement(9389..10063)
                     /locus_tag="VFC43_04005"
     CDS             complement(9389..10063)
                     /locus_tag="VFC43_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30383.1"
                     /translation="MVISTSDNLRTLFEKIRSLGFFGRLFGWNRIRVISAAAGVEHTT
                     LNNELVAVYDQNRQIRNQLRSVFQDLEHQKSLVSDLRSEHAGLQNSNVHLSQTVREMD
                     EELGILRQSESKNPKRIADLERESERLRLTIEQYKTQLQKKETELGALRESEEKIKLR
                     INEIEREAAKARGPTEDYSRTIEECTSKIRDLTEENEQKTKRILELEKDADRYRMTMK
                     QYIEKL"
     gene            complement(10204..10998)
                     /locus_tag="VFC43_04010"
     CDS             complement(10204..10998)
                     /locus_tag="VFC43_04010"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HZK30384.1"
                     /translation="MDPEVIPPVFVSHGAPTLTLEHVPARQFLVDLGSRYRSVRAVLC
                     ISAHWNTLRPCVNSAKTHETIHDFYGFPPELYRIEYPARGSPDLASQVAESLRSAGLP
                     CDVDPKRGLDHGAWVPLRLMFPRAEVPIVQLSIQQSLDPASHFALGEAIREVSESAVL
                     VLGSGGAVHPLGNPRALLGPGAKTDEWAIEFNEWLIRAVTKGDRGSLVNYRSLAPYAK
                     EAHPYPDHYMPLLTTFGAAGSNARGKVLHQSWDLGDLGMAAFEFMQ"
     gene            11286..13268
                     /locus_tag="VFC43_04015"
     CDS             11286..13268
                     /locus_tag="VFC43_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZK30385.1"
                     /translation="MGPKDSWNWEDIRAGARRTIIGARKNIEDPSIFHKIALIPVLAW
                     IGLGADGVSSSSYGPPEAFIALGEHTYLAIFLALGTAFTVFIISYAYSLIIEHYPSGG
                     GGYIVATHTISQRAGVVSGSALLIDYMLTITVSIAACADAIFSFLPYSYQPYKLLAAC
                     LLIIILIEMNLRGVKESIITLAPIFLVFIAAHALLLGYGIFAHAPQISVVADGFQTGL
                     SQDLAAVGIIGVLAVFLRAYSLGGGTYTGIEAVANGMQIMREPKVHTGKRTMAYMALS
                     LAILSGGLFLCYLLWNVRPVPGQTLNAVLATNVFGGWPFGGVLALVTILSEGALLFVA
                     AQTGFIDGPRVMANMAIDSWLPRSFALLSERLTMTNGILLMGGAALFLMVFSHGSIVF
                     LVTMYAINVFITFSLSQFGMARFFYKNRSTEKKWKTHLPVYIIGFMLCSTILVITIYE
                     KIGEGGWLTLFLTSALVGLCFLIHGHYKKVEKGLKNLDTLLEVIPSPGSKNTDPVNPH
                     KMTAVQLVSGYHGFGVNTFYSIAQNFPGVYENFIFVSIAVVDSGTFKGTAEVIALSDS
                     TRNSLMKYVDLAREAGFAADYRLDIGTDIVDVAVPLCKNIAKEFPNSTFFTGQIVFRH
                     ENPFQKILHNETAFAMQRRLQYSGITTVIMPVRAEK"
     gene            complement(13332..13700)
                     /locus_tag="VFC43_04020"
     CDS             complement(13332..13700)
                     /locus_tag="VFC43_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30386.1"
                     /translation="MKKGGILLVSVFCSILIAGCIQLPGTHFFSDSPDPVIGQWIGGE
                     QPASDRHIIFYENQTYYTSDFFMNRGEMTDTGTWTKKEPGLYVTKNVSGETTDWIYDS
                     FDDSVYMSGLPQMEYHRFKG"
     gene            13791..15083
                     /locus_tag="VFC43_04025"
     CDS             13791..15083
                     /locus_tag="VFC43_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HZK30387.1"
                     /translation="MDTLLAFVITLALITVISIRLRISPFFTLIGGSILFGLLAGLTL
                     DATLLGVVAGIGKVFSAFGIIILCGAVIAKLLQEQHQTEEIVADIRHRIRNPRALAGL
                     SGYLLAVPITCCITAYIMLNPILDSLEKDPKKRAVLLYLSAIGAIISYALVYPTPVVI
                     PLFDAFSGGMNPLIFDAVTIPVSLVTLAGILLFFKWWCPDDMATGTNQLPIAADKSRK
                     TATTAGTSPSFHPRAWAPFIAIVLSIPVALFILQLSHMSMINFIMLIGAVTAIALAPR
                     DARSAGLSQGAKHAGIIIFDICGAGALGFVIVQSGFAQAALLQLTLFIPIILVPFLLA
                     ACIETAQGSRVVTAVITAEVLAGSAVAGAIHPLPLILLISAGSCVISYVTDPFFWLVQ
                     RTTGDDVRTVVRNYTIPVALAGIFLFIVALGLEYFVFQ"
     gene            complement(15183..>15575)
                     /locus_tag="VFC43_04030"
     CDS             complement(15183..>15575)
                     /locus_tag="VFC43_04030"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZK30388.1"
                     /translation="LYAEQLQAFEKAVSLEPDNAEAWFNKGYALAGMKRYDEAIAAFD
                     RVKVINPSYPNLQKNREIAVQLRNKAGTPSGTTGTAGQTTTTPSLSVSPEKTSQPPTG
                     TPARPSPLTGETGIIALVCIVFLLVPRK"
BASE COUNT         3710 a         3852 c         3763 g         4250 t
ORIGIN      
        1 ttcgctcgat catgtaatta ccgtcatgag cgaacggcat gacaaagttg ttgtgctttg
       61 tcatgccgtt cgctcatgac ggtaattaca tgatcgagcg aatggaatcg gctgactgtg
      121 gccacatcct ccattatgtc cctgaccgga caggtaagat tccggacaag ggacgatttc
      181 atcagatccg tctttgtgac gatccccaaa atttttcctg cctcgataac cggaactgaa
      241 ctgatatttt ttttagcaaa aatgccggcg atcgttttaa taccggtatc aggcgcaaca
      301 acaacgggac tggctgtcgc aaacgcctcc accgggatct ggtctatagg acggcggcgc
      361 catgcaggct ctccctgccg tatatggtat gcgatatctt ttttggtgag gatgccagtc
      421 aactgccctt cttccatcac aaggagacgg gatattttat gtttcaccat caggttcctc
      481 gcgtaagcga tcgtctcttt tggggagaca aaaaaaaccg tatctgtcat cacatcgcgt
      541 gcaaacatgg ctatcaccct ttggcaaatg ccctgacaag atcaaactcg gttaccagac
      601 caatcagctg cgatccctcg attaccggta atgcacctat atttctggtc agcatctcgc
      661 gggctgcctg gttgatgttc tgcatcggcg ttgtcgtgaa gaggtccccg gccacaagag
      721 tccggaccgg tagtcccata acctcatcga tattaccggt agtcaggcgc gaaaagacct
      781 cgcagctccc gagatatttc atgatatcgg ttgcagtcac catcccatag aggacatcgt
      841 cgctcacgac cggcaatctc cggaaatggt accggatcat gtcgcgggcg acttcagcga
      901 tggggcagtc cggtgcagta accctcaacg atgaactcat aatatcctcc acgtttttgg
      961 tactccggtc tgctccaagg accctcagca catcccgttc ggtaacgata ccattgagga
     1021 caccttcatc atcaacgatt ggcatccccc cgatcttttt ttcaacaatc atggtaactg
     1081 catcgctgat ccgtgattca tgggagagcg tggtggtctg ctgggtcatg atggtacgga
     1141 cactctcgtt gactgcagcc cgcaggttgc ccccatgtcg tacctgcacg agtttgaact
     1201 tgtctcctcc tcccatgaaa ttgataatat cacccgatgt gataatcccc cgcagttttt
     1261 ttgtgccggc atcggttaca ggtaaacggc ggaaaccgca ggatgtcatg atctctaccg
     1321 caccgataat gctctgggta ggcgggacgg agatgacatc ggtagtggca atcgccatga
     1381 tctcaccttc ctgctggaca atatgggatt tgaactctac cgggccgcgg tcgagttttc
     1441 cctgcatctt taacagtctg tcgccttgtt tgatgctccg gtcattctgg tgcattctgc
     1501 cctccgaata attcaatgac cctggtactc tctttttgtt ttgttccggc gtgctggtta
     1561 ttccaaattg tctgtacacg ccatcttgcg gaatggatca cgcaagacct ctcaggacat
     1621 cgtgccggtc gacaattccc acgatccggt ccccgtcagt taccggaagc cggcttacgt
     1681 catgttttac cagcagctct gctgcagagc cgattggctc gtcaggacta atcgtgatca
     1741 catccttgga cataatctct tctatcgttg tctgggcatg atgggcaatt actgactgga
     1801 cccgccgtgt ccgtatgagg tcgcggcggg agatcagccc gacaatccgt tttttcttca
     1861 ccacaggaaa tgcggaaaaa ccactttcca taatcatggt gtaaatcttc tggatcgtgt
     1921 cggtcggact tgcaataatt acctccgtgg tcatatggga agcgaccgtc ccgcgaagtt
     1981 cgtgacggga cgttattacg gggaaaacat ccgaaagaag tacgccgccg attatatgcc
     2041 tgctggaatc aacgaccgca acactgtcag tctcgttatc ccgcatcacc cgcgcaacct
     2101 gctcgatgct gtcagcaggg cttacagtgg gtccttcctt gatgtagcct tccacagtta
     2161 cgttcgattt cgtagccgtt accctcaacc cgtccgtcag gtcaatatag ccgaggatgt
     2221 tttttttggc atccacgaca tagacttcgc ggaaccggtc atctctcagg atttgccgtg
     2281 ccttggttat ctggtcattg tatttcagca caggaatccc aatcatgaaa tcctgtgcgg
     2341 ttttcatgca atattgtcct cttcacggca acgggtacag agcatgacat tatcaacccg
     2401 cttgagatca tcagacatct ggctgcagcg attgcacatc ccctgctcga caatctcctc
     2461 gcggttaatc tcgatgagat cttctaagag ctcattgagt tcgttggaaa cggtaaggag
     2521 gtcccggaca gttacaattc cgatgacctt cttctttgca tcgataacgg gcagtctcct
     2581 taccttatgc ttcaccatca tgtgtgcggc atcgccgaca gttttctccg cactcaccgt
     2641 aatgagtggc gtgctcatga tttccctgac ctgtacagta cttggtttga gatcttttgc
     2701 cactaccttg caggtaatgt cttcctcggt cacgataccg gttggcttat tgttctcaag
     2761 aataataaca ctgccaactt catcggtaca catagctttt gctgccatcg caacattggc
     2821 ctcgatacca atcatggtcg ggttgttttt cattacatct cgtagcggaa cctgggtttc
     2881 aaaccgcatt ccattggttg tctttttcat gagtcacctc cttgaagtgc tgttcctgat
     2941 gattacaatt agcatactcc ccttcacttg tataaataca atttggcgaa ggcaaccagc
     3001 gatgcagcgc agtaatatga gtgtgtgccg gtaactgccg ccggtgagta aaaaacgccg
     3061 tagtataaga aacataataa agagcgattt ctatacacta ctaacgaatt atggtgaatt
     3121 ctttgaacct ttgtacgttg cgtggtcatg gaggcttccc atgaaaatat tttatcagac
     3181 ctatggtacg gcaaagaaaa aattttccag tttcctcaaa aggtttttta aaaagaagag
     3241 aacccgtatc ggtatttatg gaccacccaa tgcagggaag accacgctgg caaaccgcat
     3301 agcccgtgac tggaccgggg atgctgtcgg tccggtaagc gagatcccgc acgagacacg
     3361 aagggcaagg aagaaagaag atattatcat ttccggtgca aatggcagca cgataaccat
     3421 ggatattgtt gacaccccgg gtgttactac caagattgat taccacgaat tcctggaatt
     3481 cggtatggat aaagatgaag cgatcatacg ggcccgggag gcaaccgaag gtgtagctga
     3541 agcgatgcac tggctgcgtg aggatatcga cggtgtaatc tacatgctgg attcaacact
     3601 ggatcccttc atgcaggtga atatcatgat gataggcatc atcgagagcc gcaaactccc
     3661 ggtaattatt gttgcaaata aaattgacct tgcagatgcc gttcccgcac ggatccgaag
     3721 cgcgttcccg cagcacccgg tggttgcaat ttccggcctc gaaggcagga atgtcgagaa
     3781 tctgtacgag actatgcttg aatgcttcgg gtgaactaaa atgccgcaca aatgtacaaa
     3841 atgcggaagg gaatacaggg ccggttccac agaaatctta aaaggctgtg caagctgcgg
     3901 gggtaaaaag ttcctctacg tgaaagaagc agagatccac agggacgtgc ttgatgagaa
     3961 gtcgatggat gagattgcag atgagtctca tgaggacatc ctcgagatcg tagaaccccg
     4021 gaaaaaagaa caggtagaga tgtatgaccg ggttgagacg atccgtattg tttcgccggg
     4081 ttcctatgag ctcaatttag aaaagatggc ccagagcgac cagcggattg tcagtgtcgg
     4141 gaaagaagga aattatatca ttgacctaat gtccatggca aaggacgaca aaaaaaagaa
     4201 gaaaggaaag aaataacccc ctgtactatt ttgtttccag ttcattcttt attccctcat
     4261 aaggcggggg gagatcaagt cttttgaaaa aatcatcggt gaatgcaagg ctgttgatca
     4321 ccgcccattc agcacagcca tgaccaaagc aggtatccgg aaaaaacggc cggagttgtt
     4381 gggctaacgg gtttggggca aatttgttcc ggagtatttt ttcaagcctt tcgccgtgcg
     4441 tctctgtgtt gttatcaggg ttatcaccct gctggacgac aatccattcc cgtttgtaat
     4501 gcatcaggta attccgtatt tcgataagag tcctgatatc tgaaaaaacc ggatcaccct
     4561 catcaaatgg gggtttatct gcaataacca gaattttttg atatttattc agaaggggag
     4621 cacgatcaaa attcttttcg tttctccacc tctcaccaat tgtttttaag agcatcccct
     4681 tttgttcatc aataaagaaa taggcatcat ccgctgcatc tgcatagagc tcattgatag
     4741 tggattccag gaatgcaacg gatgagagga ccgagttgag gatgtatgcc tcgtgacgca
     4801 gatgttcttc ttcagatatc attccactcg acgcaaattc cctttcaatc cggtacccga
     4861 gcctgcataa caatgcagca gactgaatgt accggacaga gaagcaatcc cggatcctta
     4921 tggccgattc atcatcgatc gtttccaggg gagttttgtt cattttacat tccttttctt
     4981 ttcatacata aaaagacagg gacggttgcc gggaccagtc ggactgatcc gctcacggtg
     5041 attatttctt ttgaatgaag catacctgaa aaaaggttac ctcagaagcg ggctcccgcc
     5101 tgaaagaaaa ggaacgccgc gataaggata atcactacag cggctataac aacatacgtg
     5161 aggggttttc ccagtcttgt ttttttctgt gatttgcgca aaagaatcct gatagtgcgg
     5221 atttcttcct gtgattcatc cactgagtca cgtatctggt ggagtaagac acacgggtct
     5281 tcagggaacc tgtttccgca atgcagacag aaagctgcag catcctctga ggtgaatttg
     5341 tcgcaggact cacacttttt taccattttt tctcaacttt tatgatggta tttctctgat
     5401 aatattctat cagggcaggc aggttaataa attattacat tttcgtttgt aaaatccaca
     5461 aaaaacgtgc gtagaagcga gttttttata gtgaacaaag agggaaaaaa gagaccggga
     5521 ttaataatct cccataccgg ggggcatgcc tccggggggc atgccaccag ctggtccggc
     5581 tgcttttgaa gacgcgatga tatcgtcgat acgcaggatc atgaccgcag cctctgctgc
     5641 acttgagatc gcctgtgttt ttacccggag tggttctaca accccggcct tgagcatatc
     5701 gacgggtttt ccttcgtaca cattcaggcc gaagttcttt ttccctgctt catgtgcagc
     5761 ccgtattgca accagcatat caatggggtc aagtcccgca ttctctgcaa gagtcctggg
     5821 gatgacctcc agtgccgagg cgaatgcctc aatggcaagc tggacacgtc cgccctcctg
     5881 cgaggcgtat cttctcagct ggagcgacag ctcggtctcg ggcgccccac caccggcaac
     5941 gatcttttta ccctcgataa caacgcttac aaccataaga gcgtcgtgca tggcccgttc
     6001 aagttcatcg acaacgtgct ctgtcccgcc cctgacgatg atagagaccg cttttggatt
     6061 tttacatttt gagacataga tcatctcatc tcctgatact ttgcgttctt cgacaagccc
     6121 ggccgagccg agatcctttg tcgttatcgt atcgatgctg ttgacaatgt tcgccccggt
     6181 tgcccgggca aggttctcta tatcgctctt ttttaccctg cggactgcca ggatacctgc
     6241 ctttgcaaga taatgctggg caacatcatc gatacccttc tggcagaaga ggacagtcgc
     6301 cccgcttttt acgactttat ccaccatcgt gtggaccatt tgttcttctt catcgaggaa
     6361 tgcctggacc tgcccaggag ttgagatctt tatggtagcg ttcacttcgg tctttttgaa
     6421 ttcgagcgct gcgttgagta aaaggacctt tgcattcatg acagatttgg gcatgctcgg
     6481 gtgtgcacgt tccttatcaa tggccatgcc ttcaatcagg ttcgtgtcgt ctactgcacc
     6541 accgaccttc ttttccacat tgatatgact gatatccact ttaccgtcgg gatcggtaac
     6601 aaacccgatt gctttcacga tgatgtcgca gatatggtct tttgttactt ctgcattctt
     6661 gccggtgaga gctgtttctg caatcttttt gagcatcacc gtgtcggagg gttttatggt
     6721 tatggaaaag ccgtcaagga tcacgagtgc ttttgttgtt gccagccggt atccttccgc
     6781 aattaccgtc gggtgaactt tttggctaag gagatcctct gcgttcttta aaagttctgc
     6841 ggcaatgaca acagcggtcg tggttccatc gcccacctcg tcatcctggg tttttgctac
     6901 ttcgaccatc atctttgcag cagggtgctc gatatccatt tcctttaaaa tggtaacccc
     6961 gtcattcgtg atggtgatgt ctcccatacc gtcgatgagc atcttgtcca tgccttttgg
     7021 accaagtgtt gagcgtactg cagctgctac tgcctttgca gcggcaaaat tattgttctg
     7081 cgcctcttgc ccacggttcc ttgttgttcc ttccctgaga ataattatgg gttgtccccc
     7141 gagttgttgc gacatgttaa tcctcattaa attgtataca ggttgttttt aatcggttaa
     7201 atatcgttac attccgttct ccccatacaa gtcacgacag cgtaatgcat ttttaacagc
     7261 atcacgattt cccggcctgt cagggacagc acaatctgcc aggaatgaac cgataaaaag
     7321 ggtagtgttg atattatttg agatgatccc gcaatttcct gaccagagcg agctggtttt
     7381 ccgcttcatg tacctgttcc ttttcaatgc aatccatgat ctcctggagg ggttttgcca
     7441 gcgtatagat tttgaccggg cggcctttgc tttccgattc gctctcgcgg gtcgttatcc
     7501 atccctgttc tgcaagatac cgcatcgcga ggctcacttc gggctggcgc atatccgtgc
     7561 cgcgttcgat ggcatgggaa gttgtttctt tggtatttgc aagaaatacc agtgctttgg
     7621 caatattttt cctggttcca atcctgataa gaagcatagc gaactcttct tcccggtcag
     7681 taaaaaattc aacgttttcc tgtctcatct gcaccaccaa aatatctttt ttagactgct
     7741 ttcaatgtct aatcgttatc ataactacat ttattctgat actatatagc aaaaagaggt
     7801 ctaaggggat tgcctgccga aaaataaaat cgagccataa aaattattga ttgccggata
     7861 atctgaaata aaacccgaat gggctctttt tctcatccca aaaccacagt taaaaccggt
     7921 agtgccactt gagtataatt tctaaaatag gggacaggga ttgggcgtat tattcactca
     7981 attcccaaat gactgcccaa cgaaatttgg ggcagtgtcc aggagcagtt ctccctgaaa
     8041 aagaattcag aaagtcacaa ttgcgaatcg ctaattcttt gtagtacgta atcaatggta
     8101 gttcacctga aaaggggaga taatcatggc aaaagcaaac atacctgaaa aaggcgcgat
     8161 cattcagagg gatatggaaa cgtattcgat ctcaccccac attcccggtg gttttatcga
     8221 gcctgcgatg ttaaggaaga ttgctgacgt ggcagagaaa tacaacgcca gatacgtgaa
     8281 actcaccggt gcccagcgca tggccatcat cggtatccgt gaagaagacc tggataaggc
     8341 ctgggcggag tttaccgacc gttccaaggc aatcggcctg accatacgga gtatccagat
     8401 ctgtcccggc acccgggcgt gcaagaaggc aaagcaggac agtcctggcc ttggattcgc
     8461 cctggaccag gagttctacg gcaagccggc accagccaag ttcaaaatgg gcgtatcagg
     8521 atgccaaaac tgttgcagcg attcctggtt aaaagacatc gggttcttcg gcactgacga
     8581 ggggttcacg gcagtcgccg ggggcaaagg cgggggaacg gcaaagccgg gccgggagct
     8641 tgccaaaggc ctgaccgcgg atcaggcagt agcgctggcg agaagggtca tcgccttcta
     8701 ccgggaaaat ggtaagttcc ccgaacggct cggtgcaacc atcgatcgcg ttggtttcga
     8761 cacgtttgta aaagcagtgt cctgaagagg acaggcaggc atcacaacaa ccttttttta
     8821 agatttcttc cttgcgatcg ggtttctaaa tgcccgcttc tgtacgataa aagaaagata
     8881 ttgggtggag ctctgaaacc agtaaaatta ttgaaatctc tgtagacacg cacatgaatg
     8941 ggagttcaca tccgaaccat atacatcgct ttctctatga tggaccccgc ctcagggcct
     9001 ctccgaggca cggcgggtag ggagtttttt agtcattaat ccgccacggg ggtgtccctt
     9061 tgggggcggc ggcatttatc ataactgatc gatagtaggg taccagttcc aaaatcgtat
     9121 ctgataaaca atgattttca ggggaattct tttgatcatt atatgaaatg aaaaggaccg
     9181 atgctttgaa gggggggcca ttgcctgtcc ttccggaccg cccctgggct gtgcaatagt
     9241 gctgttatta tcttctctgg agttatcgaa tcgggggggc caagtggcgg gggtgaaacc
     9301 ccaatgaacg ttcaagtttt ttgaatattt ctgctgaccc tattttttat attttctttt
     9361 ttttaaaact tcggttatac gacattcgct aaagtttctc gatatattgt ttcatggtca
     9421 tccggtaccg gtccgcatcc ttttcaagtt caaggatacg ttttgttttt tgttcattct
     9481 cttcggtcag gtcacgaatt tttgaggtac attcctcaat tgtacgggaa taatcttccg
     9541 tgggtccccg ggccttcgct gcctcacgtt cgatttcatt gatccgcaat ttaatttttt
     9601 cctcagattc cctgagcgca ccaagttcgg tctctttttt ctgaagctgg gttttgtatt
     9661 gttcgatagt taaacgcagg cgttctgact cccgttccag atcggcgatt ctttttgggt
     9721 tttttgattc tgactggcgg agaatcccaa gctcctcgtc catttccctg accgtctgcg
     9781 ataaatgcac atttgaattt tggagacccg cgtgttcaga tctcaggtca gataccaggg
     9841 atttctggtg ttctaagtcc tggaaaaccg aacgcagctg gtttctgatc tggcggttct
     9901 gatcatagac agccacaagt tcgttattaa gtgtcgtatg ctcaactcct gcagcagcag
     9961 aaatcacacg aatgcggttc cagccaaata atctcccaaa aaaaccaaga gacctgattt
    10021 tttcaaataa tgttcgtaaa ttatccgaag ttgagataac cattattcac accctttgta
    10081 ctatccaata atgaataaaa cagttactat tatttataaa gtcccttccg gaattattat
    10141 tataaagata gatcaaacct tttttttggt ataactaact actttccgcg atattttatt
    10201 gatttattgc atgaactcga atgctgccat gccgagatcc cccagatccc agctctggtg
    10261 aaggactttt cctcgtgcat tggaccctgc tgcgccgaac gtggtgagaa gcggcatata
    10321 atgatcggga tacgggtggg cttcttttgc ataaggagca agcgaccggt agtttaccag
    10381 gctcccgcga tctccttttg taacagcacg aatgagccat tcattgaatt ctattgccca
    10441 ttcatcggtt tttgcacccg gtcccagtaa tgcccgggga ttacccagcg ggtggactgc
    10501 tcctccactg ccgagcacga ggaccgctga ttcgctcacc tcccggattg cttcaccaag
    10561 tgcgaaatgc gaagccgggt ccagggattg ctggattgag agctggacta tgggcacttc
    10621 agcccgggga aacatcagtc gcaggggcac ccacgcacca tggtccagac cgcgtttggg
    10681 atcaacatcg catgggaggc cggcggacct cagggactct gctacctgtg aggcgagatc
    10741 gggacttcct cgtgccggat attcgatacg gtacaattcc ggcggaaacc cgtaaaaatc
    10801 gtggatcgtc tcgtgggttt ttgcggagtt aacgcagggc cgcagcgtat tccagtgtgc
    10861 agaaatgcat agtactgccc ggactgaacg gtaacgggag cccaggtcca caaggaattg
    10921 ccgtgccggg acatgttcaa gcgtaagcgt gggtgcgcca tgcgacacaa acacgggggg
    10981 aatcacttca gggtccattg aacattacct tggtgatgct cttttttagt tgggaaggaa
    11041 ataggtgccg atgatgacaa tccgtggatg gtggacacaa tttttgggtg taggcatttt
    11101 tgggaatcct gacctgaata atttcataaa tatgtaaata tataaatacc agaagattaa
    11161 taagccagct tccaatacag tttctcttga gtgcatagcc gaaattggtt ttaactattc
    11221 caacgtgagc gacaggtcct tgttgactca gttatgaacc tgtccagctg caggaagaag
    11281 ataacgtggg acctaaggac tcgtggaact gggaggatat ccgggcaggt gcccgccgga
    11341 ccatcattgg ggccaggaaa aacatcgaag atccgtcaat tttccacaag attgcattaa
    11401 taccggttct tgcatggatc ggccttggag ccgatggagt ttcctcatca tcgtacggac
    11461 cacctgaggc gtttattgct ctcggtgaac atacgtatct cgcgattttc ctcgcactcg
    11521 gtaccgcatt tactgttttt attatatcgt atgcatacag cctcatcatc gaacattatc
    11581 ccagcggtgg cgggggatat atcgttgcta cccatacaat cagccagcgg gctggtgtgg
    11641 tctcgggaag cgcactcctc attgactaca tgctgaccat cacggtatcc atcgcagcct
    11701 gtgctgatgc gatattctcg tttttaccgt attcgtacca accgtacaaa ctcctggcag
    11761 cctgcctgtt gatcataata ctgattgaaa tgaacctcag gggggttaaa gaatcgatca
    11821 taactcttgc cccgatcttc cttgttttca ttgcagccca tgcactgctc cttggttacg
    11881 ggatttttgc ccatgccccg caaatcagcg ttgtcgctga cgggttccag accgggctct
    11941 cccaggattt ggcggcagtc ggtatcattg gagttctggc cgtattcctg cgggcctatt
    12001 cactcggtgg cggaacctac accgggatcg aggcagtagc aaacgggatg cagatcatgc
    12061 gcgagcccaa ggtgcacacc ggcaaacgca cgatggcata tatggcactc tcactggcaa
    12121 tcctttccgg gggcctgttt ctctgctacc tgctctggaa tgtacgcccg gtcccgggtc
    12181 agaccctgaa cgctgtgctg gcaacgaatg tttttggtgg gtggcctttc ggaggggtcc
    12241 tcgcgctggt cacgatcctt tcagaggggg ctctgctctt cgtggctgcc cagacggggt
    12301 ttatcgacgg gccacgggtg atggcaaata tggcaatcga ttcgtggctg ccccgatctt
    12361 ttgcactgct ttctgaaagg ctcacgatga caaacgggat ccttttgatg ggcggggcgg
    12421 cgctgttcct catggtcttt tcgcatgggt cgatcgtctt tctcgtcacg atgtacgcca
    12481 tcaatgtgtt tatcacgttc tccttatccc agttcggcat ggcacgattc ttttataaaa
    12541 accggtcaac ggagaagaag tggaaaacgc atttacctgt ctatatcatc gggtttatgt
    12601 tgtgcagcac aatcctggtt atcaccatct atgaaaagat tggtgaaggg ggctggctca
    12661 cgttatttct gacatctgcc cttgtggggc tctgcttcct gatccacggg cattacaaga
    12721 aagtagaaaa gggcttaaag aatctggata cgctgcttga ggtaattcct tcacccggca
    12781 gtaagaatac tgacccggtt aatcctcata aaatgactgc agtccagctg gtttccggct
    12841 accatggatt tggggtaaat accttttatt ccattgcaca aaattttccc ggggtgtatg
    12901 aaaattttat ttttgtttcc attgccgtgg tagattcagg taccttcaag gggacagcgg
    12961 aagtgattgc actttcggat tcaacaagaa attcgctgat gaaatacgtt gatctcgccc
    13021 gtgaggcagg ttttgctgca gattaccggc tggatatcgg aaccgatatc gttgatgtgg
    13081 ctgttcccct ctgtaaaaac atagcaaagg agttcccgaa ctccacgttc tttaccggac
    13141 agatagtctt ccgtcatgaa aatccgttcc agaaaatcct gcataacgag acagcgtttg
    13201 ctatgcaacg acggttacag tacagtggta taacgacggt aattatgcca gtccgtgccg
    13261 aaaagtagtt ttttagttca aaaacctgaa aacgtaacaa aggctctagg atttttgcct
    13321 gttactcact tttacccttt aaagcggtgg tattccatct ggggcagtcc actcatgtac
    13381 acagaatcat caaacgaatc gtaaatccag tcggttgttt caccgctcac gtttttcgtt
    13441 acatagagac cgggttcttt ctttgtccac gtgcctgtat cggtcatttc accccggttc
    13501 atgaaaaaat ccgaggtata ataggtctgg ttttcataaa atatgatatg ccggtccgat
    13561 gccggctgct cacccccaat ccactggccg ataacaggat cgggggaatc actgaaaaaa
    13621 tgtgtaccgg gaagctggat acaccctgct atgaggattg agcagaagac ggaaaccagg
    13681 aggatacccc cttttttcat aagtgagatt gaataagtct gacacgttta agctttccct
    13741 ggttttagcg tatccaagcc tattttttat cagctgcaag aatagtatgt atggataccc
    13801 tcctcgcgtt tgtgattacc ctcgcactta tcaccgtgat cagtatccgg ttgaggatct
    13861 caccgttctt cacgctgatt ggaggttcga tcctgttcgg gctcctcgca gggctgaccc
    13921 tggacgcgac cctcctgggg gttgttgccg gtatcggaaa agtgttttcg gcatttggca
    13981 ttatcatcct gtgcggagcc gtcatcgcaa aactgctcca ggagcaacac cagacagaag
    14041 agatcgttgc tgacatcagg catcgtatcc ggaatccccg agctctcgca ggactgtccg
    14101 gatacctgct ggcagtgccc ataacctgct gtatcactgc atacatcatg ttgaacccga
    14161 tactcgacag cctggaaaaa gatcccaaaa agcgcgccgt tctcctgtat ctttctgcaa
    14221 tcggggcgat tatctcttac gcactggtgt atccgacacc tgttgtcata cccctgttcg
    14281 atgcgttctc gggcggtatg aaccccctga tattcgatgc cgtcaccatc cctgtttctc
    14341 tggttaccct tgcagggatc ctgctttttt tcaagtggtg gtgtcctgac gatatggcaa
    14401 caggtacaaa ccagcttccc atagcagctg acaaaagccg aaaaactgcc acaaccgcag
    14461 gcacatcgcc ttcgtttcac ccgagggcat gggcaccctt cattgccatc gttctatcaa
    14521 ttccggttgc cctttttatt cttcagctgt cccatatgag catgattaat ttcattatgc
    14581 ttatcggagc agtcacggcg attgcccttg cacccaggga cgcccggtcg gcgggtttgt
    14641 cacagggtgc aaaacacgca gggattatca tttttgacat ctgcggggcc ggggcactcg
    14701 ggtttgtcat cgttcaaagc ggatttgcgc aggcagctct tttgcaactc acgctcttta
    14761 ttcccatcat ccttgtaccg ttcctgctcg cagcatgtat tgaaaccgca cagggttccc
    14821 gggttgtgac agcggtcatt accgcagaag tgctcgcagg ttccgcggtt gccggggcca
    14881 ttcaccccct cccgctcatc ctcctgatca gcgccgggtc atgtgtcata tcctatgtaa
    14941 cagatccctt cttctggctc gtccagcgca ctaccggtga tgatgtcagg acggttgtga
    15001 ggaattacac gatacctgtc gcactggcgg ggatatttct cttcattgtt gcacttggac
    15061 tggaatattt cgtcttccag tgacgccaca attcgaagaa acctgcacaa aaacatttcc
    15121 ggtgaaaaag gaggaacagg ttgttttttc cggatagaac tgttgtgccg gtaaaatgat
    15181 agttattttc gaggcacgag caggaataca atgcagacaa gtgcaataat tcctgtttca
    15241 ccggtaaggg gtgacggccg ggcaggagta ccggttggcg gttgtgaagt tttttccggg
    15301 gataccgaca gcgatggagt tgttgtcgtc tgtcctgccg ttcccgttgt tccagaaggt
    15361 gtgcccgcct tattgcggag ctgcacggca atttcacggt ttttctggag attcgggtaa
    15421 gaaggattaa tgacctttac ccggtcaaat gccgcaattg cttcatcata ccttttcatc
    15481 ccggcaaggg cgtatccttt attgaaccat gcttcagcat tatcgggctc aagagagact
    15541 gccttttcaa aagcctgcag ttgttcagca tacaa
//
LOCUS       DASRLV010000099         7670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_765893,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000099 DASRLV010000000
VERSION     DASRLV010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 7670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7670
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765893"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            291..458
                     /locus_tag="VFC43_04035"
     CDS             291..458
                     /locus_tag="VFC43_04035"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HZK30389.1"
                     /translation="MVSFKCKDIGMDCKYETTAANVQELDDKVAQHAREAHKIASLDK
                     DMWMKIHKAEK"
     gene            609..1127
                     /locus_tag="VFC43_04040"
     CDS             609..1127
                     /locus_tag="VFC43_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HZK30390.1"
                     /translation="MTETIAYKDSGRMGEVEIRTYPAIILATVQGLAEGEAFGILFQY
                     ISGNNKPGRAIPMTAPVITLEKIAMTAPVISNADAMSFVLPAKYPRDEVPEPLDPHIS
                     IQEISPRVLAVIRFKGRASDYDVAEVTGRLLKVLESENIPIVGKPFLMRYNSHFTPGF
                     LRRNEVGIEIRQ"
     gene            1205..1681
                     /locus_tag="VFC43_04045"
     CDS             1205..1681
                     /locus_tag="VFC43_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZK30391.1"
                     /translation="MDPVKIPKMAKAEYDALIERQYVSRISFGACDHPYIAPFMYVFD
                     KKYLYFLSTKYGRKIEYFAANPKVSVEIEEYAPDLSVFTFVSLQGVLEEVKDPAQKRK
                     VREQFVEMIAKNRLSSHVLSALGHSHNDPPDVIVREERSLVWKLVGVKDIVALKNG"
     gene            1856..3628
                     /locus_tag="VFC43_04050"
     CDS             1856..3628
                     /locus_tag="VFC43_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZK30392.1"
                     /translation="MAQWKCTVCGYIYDETMGDPKGGITKETPFEKLPDTWTCPVCGA
                     GKDAFVKVQTEDIHAGSATTVSDVMVAELAAWGVNVVFGLPGTSSLGLVEAIRKNHDI
                     RYFVVRHEENAAFAASAYNKFTGRIAACLTIAGPGATNLATGLYDAKEDHASLLSLNG
                     QVEAQYTGPGGVQEIDQDAFFRPITVYNNTIYEKNLSLLLLTRALRYARIDRGVAQIS
                     VPNDIQKKPLDLRFCTRESSLGGPDIVPPDEKIKTAASAINNAKKPVILAGWGAYPDG
                     QQVLALAEKINAPILTTFRAKGILPDDNPWVIGILGNVGSPQARILVNEADLVITLGV
                     GFSKYTNVPTEKSFVQVDIDPLKLGKNAHTIALWGNCSLLLPKLYPLLNEREDRGIKD
                     RLAAMKKQWDQQRDAEADSSAAPLRPPFIMKVLSEVIPADAVISLDVGENQWWFGRNF
                     RMKQQRFAMSGYLATMGFGLPGAIAAKIAYPDRFVFCITGDGGFSMAMADFITAVKYN
                     LPMVVVILNNHQLGMIQVEQMMEHYQNYATDLLNPDFSRYAEACGGLGIHVSRPEELR
                     TALLTAMSAERPVIIDVETDPKRF"
     gene            complement(3903..5384)
                     /locus_tag="VFC43_04055"
     CDS             complement(3903..5384)
                     /locus_tag="VFC43_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HZK30393.1"
                     /translation="MTLYRCDVCNVFEYDSEQGEPVSHILPGTQPENFPADWACPVCN
                     SDSVHLIPVLSPAAILSPNTCPSCGSPLPARVGSPADTKVLQDYLSPWERHTDSFEEY
                     LADIHMMAVTGKSIIEPMRTKKPVISWDDILIKGAQLATLPLNKEMSVNTRTVIGPKA
                     RQPMVIETPVFVTHMSFGALSKEVKIAIAQGTAAVRTAMGSGEGGIVPEARSQAYRYI
                     LEYVPNRYSITEENLRSADAIEIKIGQSAEPGMGARLPAEKVTDEIAKIRGYPQETDI
                     ISPASYDDIRNREDLKKKVQWLREMSGGRPIGIKIAAGEIEADLAEVVYAEPDFITID
                     GRPGATGAALKYIKNATSVPTIFALYRARKYLDRNNIRDISLIITGGFRVSSDFAKAL
                     CLGADAIAIGTAALIACACQQYRICHTGNCPVGVTTQRPDLRERLKPELSAKRLEQFL
                     NVSTEELKDFARLTGNRDVHALSTRNICTTNSEISDHTGIPHV"
     gene            complement(5498..6115)
                     /locus_tag="VFC43_04060"
     CDS             complement(5498..6115)
                     /locus_tag="VFC43_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HZK30394.1"
                     /translation="MNDEVPKIPVFMQQMAETITKTACGLDLDQMSAFFQVMVSAERV
                     YVAGAGRSGFIAKAFGLRLLHLGYDVYVVGETITPALHAGDTMVVFSGSGETHSMVSI
                     SGTVKDLGGTVCLITASSDSTISKIADCIVNLGDLTGYYRKGGSPFEIRQLTGEYRSV
                     STAFAPLGTLFETLALVFSDAVISGLIEVRKAEISDMQRRLANIE"
     gene            complement(6209..6853)
                     /locus_tag="VFC43_04065"
     CDS             complement(6209..6853)
                     /locus_tag="VFC43_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5591 domain-containing protein"
                     /protein_id="HZK30395.1"
                     /translation="MDDTPDKKDRLTGLGSLLTDPPFYLPEFEKSYRYIIDEYEIPQK
                     DIAIFMPCAIRKPYSKSPSHQLIRSVISQVLDDSRYHLVIFGTCGIVPAELEEMYPYA
                     HYHYMLGKCKDPAVLEDFLRIETGRVAGYLEKTRHAYKYRIGYCIGLFRQALVLGSER
                     AGVPLDLLLPTRDMINRVIEEGDCIFEEGSLSMEEYLGEFCDELIKFRNAHPPE"
     gene            6990..>7670
                     /locus_tag="VFC43_04070"
     CDS             6990..>7670
                     /locus_tag="VFC43_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HZK30396.1"
                     /translation="MARLAVVGVGRVGGEVACLATSLGIVDDLVLYDSAPEVLRAQVL
                     DLEHTGLEIPISTDTSDIREADVCIFSAGLPRSPTVRTRADLLEVNLSAARDAIGLLR
                     GFSGILITVTNPMDANNFLLCTKSGIGQERCIGFGGQLDSARFGLALRNRNITGFPFV
                     LGEHGEHQVPIFSSLGSTVENNQREEILSELRGASMQVIKGKGGTVFGPALHLAHLTR
                     IILKDAREL"
BASE COUNT         1897 a         1941 c         1947 g         1885 t
ORIGIN      
        1 ggactgccaa taagcgtccg gagcctgctg ttccctgcca tgatgataat cgtatcaggt
       61 tcagggtatt aaacctgtgg aagatgccgg atcctgcagg atgcggtcag ggagaaaagc
      121 aggatatcgc aggaattcat cacgcaaacc ggctcctttt ttgagggtca cccgggaaac
      181 taaaagaatc ccggttatcc tgagagatac ctgccagtct tttcattttt tataaagttt
      241 cgataggtat aaatgaatca ccgggcatag gggtttcccg gtgaaacacc atggtatcgt
      301 ttaaatgcaa ggatatcgga atggactgca aatatgagac cactgcagcc aatgtacagg
      361 aattggatga taaggttgcc cagcatgccc gtgaggctca caaaattgcg tctcttgaca
      421 aggacatgtg gatgaagatc cacaaagcag agaaataatt cttttttcgt ttttcctatg
      481 cagcagctgc cattttcagg gataacgttg ttttcggaga tagagcacct gatgataatc
      541 gggcgatttc cctgtacatc ccggcaagta atctgcaggg aaaacctaat gcatgcaatg
      601 gagggaccat gacagaaaca atcgcataca aggattcagg acgcatgggg gaagtggaga
      661 tccgtacgta tcctgctatc atcctcgcca ccgtgcaggg tctggcagag ggcgaggcat
      721 tcggcatcct ctttcagtat atctccggga acaataaacc aggaagggca atccccatga
      781 ccgccccggt cataacctta gaaaagatcg caatgacagc gcccgtcatt tcaaatgcag
      841 acgccatgtc atttgtactg cctgctaaat accccagaga cgaggtcccg gaacccctgg
      901 acccccacat cagcattcag gaaatttccc cccgggtcct ggcggtaatc aggttcaagg
      961 ggagagcttc tgattatgat gttgctgagg taactggacg gctcttaaaa gtcctggaat
     1021 ccgagaacat tccgatcgtg ggaaaacctt tcctgatgcg ctataactcg cacttcacgc
     1081 cgggtttcct gcgcaggaac gaagtaggaa ttgagatcag gcagtaggat tagaatcctt
     1141 ctgcgtttga taatcattat aatccgactg aacaagacta ctcatttaat ggaatgatat
     1201 tcgtatggat ccggtaaaaa ttcccaaaat ggccaaagca gagtatgatg ccctgatcga
     1261 acgccagtat gtatcaagga tctcgtttgg cgcttgtgac cacccctata tagccccgtt
     1321 catgtatgtc tttgacaaaa agtaccttta ctttctctcc actaaatacg gcaggaagat
     1381 agagtatttt gccgcaaacc caaaggtctc tgtcgagata gaggagtatg caccagacct
     1441 ctctgttttt acctttgtga gcctgcaggg ggtgcttgaa gaggtgaaag atccggcaca
     1501 gaaaagaaag gtacgcgagc agtttgtgga gatgattgcg aagaaccgtc tttcgtccca
     1561 cgtactctct gctcttggcc acagccataa tgaccctccc gatgtaattg tccgcgagga
     1621 gcgttcgctc gtatggaaac tggttggggt aaaggatatt gttgccctga aaaacgggta
     1681 acacccccct attatttgcc acttcaccgt gttttcctgt aaataatcta tcaaaccagt
     1741 acgattggcc cgatgaggac tttccagagc acatgctcat ggaattcaca ttccatgatg
     1801 agattcatgt aggagtggat agttactatt acccggtagc aaagagggaa ctaaaatggc
     1861 gcaatggaaa tgtacagtat gcgggtatat ctacgatgaa acaatgggtg atccaaaggg
     1921 agggataacc aaagaaaccc cgtttgaaaa acttccggat acctggacat gcccggtctg
     1981 cggagcagga aaggacgctt ttgtaaaagt gcagaccgaa gacatccatg cgggatcggc
     2041 cactacggtc tccgatgtga tggtagctga gctggcagca tggggagtga acgttgtgtt
     2101 cggtctcccc ggtacatctt cactgggcct tgtagaggcg atccgtaaaa atcacgatat
     2161 ccggtatttt gtcgtgcgtc atgaagagaa tgcggcgttt gctgcatccg cttataacaa
     2221 attcaccgga aggattgcag cctgcctgac aatcgccggc cccggtgcaa ccaatctcgc
     2281 taccggactc tacgatgcaa aagaggatca tgcatctctc ctgtcattga acgggcaggt
     2341 ggaagcccag tataccggtc ccggcggtgt tcaggaaatc gaccaggacg cattcttccg
     2401 cccgataaca gtctacaata atacgatata tgaaaaaaac ttatcactcc tgctgctgac
     2461 acgggctctg cggtacgcaa ggatagatcg gggcgttgca cagatctcgg tccccaatga
     2521 tatccagaaa aagccgctgg atttgcggtt ctgtacccgg gagtccagtc tcggcggacc
     2581 tgatatcgtg ccccctgacg aaaagataaa aaccgccgca tcagcgataa acaatgcgaa
     2641 aaagccggtt atcctcgccg gatggggtgc atatcctgac gggcagcagg tgcttgccct
     2701 ggctgagaag atcaatgccc cgatcctcac caccttccgg gcaaaaggga tccttcctga
     2761 tgacaacccg tgggtaattg gcattctcgg gaatgtaggg tctccccagg cccggatcct
     2821 ggtcaacgaa gccgatcttg tgatcaccct tggtgtagga ttctcaaaat ataccaatgt
     2881 cccgacagaa aaatcctttg tacaggttga catcgatccg ctgaaactcg ggaaaaatgc
     2941 ccataccatt gctctctggg ggaactgcag cctgctgctc ccaaaactct accccctgtt
     3001 gaacgaacgg gaagaccgtg gtattaagga tcgccttgct gccatgaaaa aacaatggga
     3061 ccagcagcgc gatgcggagg ctgacagcag tgcagcaccg ttgcgccccc cgtttatcat
     3121 gaaagtgcta tctgaggtaa ttcccgccga tgcagtcatc tcgcttgacg tcggtgagaa
     3181 ccagtggtgg tttggccgga acttccgcat gaaacagcaa aggtttgcga tgtcaggcta
     3241 tctggcaacc atgggatttg ggttaccggg ggcaatcgcg gcaaaaattg cgtatcctga
     3301 ccgttttgtc ttttgtatta ccggtgatgg gggattttcc atggccatgg cagatttcat
     3361 aacagcggtg aaatacaacc tgccgatggt agtcgttatc ctcaacaacc accagctcgg
     3421 catgatccag gtcgagcaga tgatggaaca ttaccaaaac tatgcaacag accttctcaa
     3481 cccggatttc tcccggtatg ctgaagcctg cggaggactg gggatccatg taagccgtcc
     3541 tgaagagctc aggactgcgc tcctcactgc gatgtctgct gaacgcccgg ttattattga
     3601 tgtggaaaca gatccaaaac ggttctgatg gggtcgaaag aaagagaagt tcacgaacgt
     3661 ttatacccgt cgctatacga tcaaccccgc cgcccccgac ggggcgcccc cgcggcggat
     3721 cagtgactaa tcaaaaactc cctgcccgcc gtgcctcgga gaggccctga ggcgggattc
     3781 ctcgtaaggg cgtgaggaca gacctggtct tcattcctct cataataaat cgtttctata
     3841 gggtctctga tacatttttt tcatgttaac gttccagaat ctctctctac tacccgaagc
     3901 gattacacat gcgggatccc ggtgtgatct gagatttctg agtttgtagt gcagatattc
     3961 ctggtactca gggcatgcac atcgcgattg ccggtcaatc gtgcaaaatc cttaagctcc
     4021 tctgttgata cgttcaggaa ttgctctaac ctttttgccg acaactccgg tttcaggcgc
     4081 tcacgcagat ccggtctttg cgtggttacc ccaaccgggc agttcccggt atgacagata
     4141 cggtactgct ggcaggcaca tgcgatgagt gctgctgtgc cgatagcaat ggcatcggca
     4201 cccaggcaaa gagcttttgc aaaatccgaa gaaacccgaa aacccccggt aattattaag
     4261 gatatatccc tgatgttgtt cctgtcaaga tattttcttg cacggtataa cgcaaagatg
     4321 gtgggaacgg atgttgcatt tttgatatat ttcagggctg ctcctgttgc ccccggcctg
     4381 ccatcgatgg tgatgaaatc cggctcagcg tagacaactt cagccaggtc agcttcaatc
     4441 tctccggcgg caatcttgat ccctatcggt cgccccccgg acatttcccg cagccactgt
     4501 accttctttt ttaagtcttc ccgattcctg atatcatcat acgatgcagg gctgataata
     4561 tcagtctcct gggggtaccc gcggattttt gcaatttcat cggtgacctt ttctgcaggg
     4621 agacgggcac ccatgcccgg ttccgcagat tgcccgattt tgatctctat agcatcggca
     4681 gatctgagat tttcttctgt tatgctgtac cggttgggga catattccag gatataccgg
     4741 tatgcctggc tgcgggcttc gggaacaatt ccgccctccc cggatcccat cgcagtccgg
     4801 actgctgccg taccctgggc aattgcaatt tttacttctt tggagagtgc cccgaatgac
     4861 atgtgggtta caaaaaccgg ggtttcgata accatcggct gacgtgcttt gggcccaata
     4921 accgtgcggg tgttgacaga catctctttg ttcaggggca gcgttgcaag ctgggcgccc
     4981 ttgatcagga tatcatccca tgagatgacc ggtttttttg tcctcatcgg ttcgatgatt
     5041 gattttccgg tgactgccat catgtggatg tcggccaggt attcttcaaa actatccgta
     5101 tggcgttccc atggggaaag gtagtcctgc aggacttttg tatccgcagg agacccaaca
     5161 cgagcgggga gcggacttcc gcatgaaggg caggtatttg gcgacaggat ggctgcggga
     5221 gatagcaccg gtatcaggtg tacagaatcg gaattacaga ccgggcatgc ccagtctgca
     5281 gggaaattct ccggctgtgt gccgggcaga atatggctga caggttctcc ttgttcagaa
     5341 tcgtactcaa agacattaca gacatcgcaa cggtagaggg tcatacagga ttaccattac
     5401 tctttagtcc gctttgggat accttaagga taatgataaa tcaaaaaaca acccgttcga
     5461 attcctttgc cgggactttt tgtacccctt cttttcatta ttcaatattg gccaacctgc
     5521 gttgcatatc ggaaatttca gctttcctta cctcgatcag cccggaaatt acggcatctg
     5581 aaaaaaccag tgccagggtt tcaaacaatg tgcccaacgg ggcaaacgca gtgctgaccg
     5641 accggtattc cccggtcagc tgccgtatct caaaaggact cccgcctttc cggtaatacc
     5701 cggtaaggtc accgagattt acgatacaat cggcaatttt gctgattgtt gaatcggaag
     5761 atgccgtaat aaggcagacc gttcccccca gatcttttac cgtgccggaa atggatacca
     5821 tcgagtgggt ctcacccgaa cctgaaaaca ccaccatggt atcaccggca tgaagtgcag
     5881 gcgtgatggt ttcacccacc acatagacat catacccgag atgcagcagc cgcaatccaa
     5941 aggctttggc aataaacccg gatcgcccgg cacctgcgac atatactctt tctgccgaca
     6001 ccatcacctg aaaaaacgct gacatctgat cgaggtcaag accgcacgct gtttttgtaa
     6061 tggtctcggc catctgctgc ataaataccg ggatcttagg cacttcatcg ttcatggatg
     6121 gtagcttgag tttttacatt cccagatatg attgtatcgt ccggacttgt cctaccgtga
     6181 atgataactg ttaccgtcat ggaaccagtt attccggggg atgtgcattc ctgaatttta
     6241 tcagttcatc acaaaattcc ccaaggtact cttccatgga gaggctgccc tcttcaaaaa
     6301 tacaatcccc ctcttcaatc actctgttga tcatgtcacg cgtgggaaga aggaggtcaa
     6361 ggggtacccc cgcccgctcc gatcccagaa caagggcctg cctgaaaagc ccgatgcaat
     6421 aaccgatccg gtacttgtat gcatggcgtg tcttttcgag gtaacctgct acccgtcctg
     6481 tttctatacg gagaaaatcc tctaaaacag cgggatcttt gcatttcccc agcatatagt
     6541 ggtaatgggc atacgggtac atttcttcaa gttctgcggg gacgatgcca cacgtgccga
     6601 aaattaccag atgatagcgc gaatcatcca ggacctgtga gatgaccgac ctgatgagct
     6661 ggtggctggg actcttgctg tacggtttgc ggatcgcaca gggcataaat atggcaatat
     6721 ctttttgggg gatctcatat tcatcgatga tgtagcgata cgatttctca aattccggaa
     6781 gataaaacgg gggatcggtt aaaagagatc ccagacccgt gagcctgtct tttttatccg
     6841 gcgtatcatc catgcttatt cctttgtcag aatggttttt ctgaatacaa gaaagaatcg
     6901 tcccttcccc gggtggatgc tggcggttca gcaggtaagc ccgtctaaag aatcacttaa
     6961 ttattatcag acgcccatag tgtatcagaa tggcacgtct ggcagtagtg ggtgttggga
     7021 gggtaggtgg tgaggttgcc tgtcttgcaa cgtcgctggg aatcgtcgac gacctcgtgc
     7081 tgtatgacag tgctcccgag gtcctcaggg cccaggtact ggatcttgag cataccggcc
     7141 ttgagatacc tatcagcacc gatacctccg atatccggga agcagatgtc tgcattttct
     7201 ctgccggtct gccccgaagt ccgactgtca gaacacgggc tgacctgctt gaagtcaacc
     7261 tgtctgcggc ccgtgatgcc attgggctgc tcagaggatt ttccggcatc ctcataaccg
     7321 taacaaatcc gatggatgcc aataatttcc tcctctgcac aaaaagtggt attgggcagg
     7381 agcgatgtat cgggtttggc ggccagctgg acagtgcccg tttcggcctc gcactcagga
     7441 accggaatat cacgggattt ccttttgtcc tgggagagca tggcgagcac caggtaccca
     7501 tcttttccag cctcggatca actgtggaaa acaaccagcg ggaagagatc ttatccgaac
     7561 tgagaggcgc gagcatgcag gtgatcaagg ggaagggtgg gacggttttt ggtcctgcac
     7621 tccaccttgc gcacctgacc cgtatcatcc ttaaggatgc gcgtgaactg
//
LOCUS       DASRLV010000100        11901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_435842,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000100 DASRLV010000000
VERSION     DASRLV010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 11901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11901
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435842"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VFC43_04075"
     CDS             complement(<1..325)
                     /locus_tag="VFC43_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZK30397.1"
                     /translation="MLYRTVPKNGDELSILGFGYMRLPLKKGGSIDEERAIRQLRHAI
                     DQGVNYVDTAPAYHFGKSEKILARALADGYRQRVRIATKLPPWSVHSREDMDHILSSQ
                     LVTLKT"
     gene            complement(488..1294)
                     /locus_tag="VFC43_04080"
     CDS             complement(488..1294)
                     /locus_tag="VFC43_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZK30398.1"
                     /translation="MSMALKQKIITKCAEQDIPLVGFAPAVRWDDPLFDPWIPLEFRP
                     RSVFPETVTVIVIGLPVSLPIVETAPSIYYHELYRTVNTLLDINGYRISLFLNTEGFP
                     SVWIPRDGYGSISILKEKPLAFFSHRHAAYLAGLGNFGINNMLLTEKFGPRVRFTSIF
                     TAAEIPPDPVIQKALCTSCMQCVNVCPVKALDGRQYPEGLTDKKSCATRSEALNKRFI
                     SPCGLCIKVCPVGEDRKLYLREDPHIYTDENGAFDKYHRAWKHVRSYGGR"
     gene            1444..1701
                     /locus_tag="VFC43_04085"
     CDS             1444..1701
                     /locus_tag="VFC43_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30399.1"
                     /translation="MAFVPDPAVLVNLLLCVIILALGYLVYRKRNSISALFIAVAFGM
                     FGLSHLSTLLAQTLFPDVTFVLLRICGYIFVAAALYLSLTE"
     gene            complement(1751..2170)
                     /locus_tag="VFC43_04090"
     CDS             complement(1751..2170)
                     /locus_tag="VFC43_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HZK30400.1"
                     /translation="MLIKTITVTSQCEGDIIDLTEKVRKAVSESGIKNGLVHLFVQHS
                     TAALTTIEFEPGVLSDLRRALTVFAPDTADYAHNARWGDGNGRSHVKAALVGPSLTIP
                     LKGGILMCGTWQQIVLLEFDVNAGRERTIVCTVIGPD"
     gene            2308..3735
                     /locus_tag="VFC43_04095"
     CDS             2308..3735
                     /locus_tag="VFC43_04095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK30401.1"
                     /translation="MKKNIPAERGNINDLSPFRHRYVVLVIVLASVLMAVIDGTVVNI
                     ALPSMTRFFSVDLSDSQWTITAYLITMTSLLLIFGRVSEYVGRARLFFVGIIIFTVSS
                     LACGLSTGLPELILFRVIQGAGAAMLFSISSALIFATTPPAERGRAMGYLGATVAIGS
                     IAGPIVGGFVVDSLGWQYIFFINIPLGILLIAAAAAYLRIDEKRTHSLSLDWHGSTAM
                     VAMFVALILTLGSLADMGMITPPAIISGVIFFLALSLFIWHERRCPAPLLDLNVFSNR
                     EFLFPVIAVVLVFVANFMMAVVGPFYFEGVLGYRPSQVGLVFLINPAVMVIVAPLAGS
                     LYDRHPARNYAALGMGITTVAFLFLSYCTLTQNVSGILVAFVLFGIGVGLFQSPNNTA
                     ILSALPKEQLSTASSVIATSRNLGMALGVSLGSILLSFQLMGAGFTGDVITADPALLA
                     VSISRIMAVSAVLCGIVVLLSSLKR"
     gene            complement(3800..6307)
                     /gene="gyrA"
                     /locus_tag="VFC43_04100"
     CDS             complement(3800..6307)
                     /gene="gyrA"
                     /locus_tag="VFC43_04100"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HZK30402.1"
                     /translation="MTSEVPPAGVPETHRTEPVSIAQEMKTSYINYAMSVIIGRAIPD
                     VRDGLKPVHRRSLFAMWEMGNTSDKPTKKSARVVGDVMGKYHPHGDASIYDTIVKMAQ
                     PFSYRNMLVQGQGNFGSVDGDSAAASRYTEVRLFPYAEALLEDLEKDTVRFIPNYDES
                     LKEPTVLPSKIPNLLVNGTDGIAVGMATKMPPHNLREVCAAVKLFLDNPEIPVEDLMK
                     VMPGPDFPTGGVMMGTEGVRNIYTTGQGRVIVRGVATIEESEGGNRGDRIIVSELPYQ
                     VNKAQWITAIAEMVKDKRIDGLSDIRDESDKDGIRVVFELRKGTMSAVILNQLYKLTA
                     LESSFWAINLAIVDNQPRVLTLHGLLENFVHHRIEVIRRRSEFDLKKAQEKVHILVGL
                     LLALSKIDLIIAAIRASDSVDNARVALITKFGLDELQANAILQMQLRRLAALEQQKIN
                     DEKKGLEAEIGRLETILSNEANIKDEIRRETVEIAAKFGDARRTEIAIDTSDLSNEDL
                     IEDKTVLISLTTANYIKRMDIDTYRKQRRGGHGITGMTTKDDDGVDRVFAAEMKDYLL
                     CFTNIGRVYWLKVYQIPESSRIAKGKPIVNLLNLKDEIVTKVIPIRVFREDRFMLFAT
                     RYGQVIKIPQTEFSNPRSVGTNAIRLKENDQLVDVIATDGSKEIVLSTRFGYSLRFHE
                     ETIRTVMRNASGVRGMKLRGEDTIVALTLVEKDHLLTISDVGFGKRTEFDDFRGHGRG
                     TMGVRNMQLERNAIVIESRAVLDTDEIIVMTASGVVIRMPVSEIRIIGRGTKGVRTIR
                     LDDKDRVVGVAIVQPEVVDSENGADAGADSSNPETNE"
     gene            complement(6297..8339)
                     /locus_tag="VFC43_04105"
     CDS             complement(6297..8339)
                     /locus_tag="VFC43_04105"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase subunit B"
                     /protein_id="HZK30403.1"
                     /translation="MTETYDASHITVLEGLTPVRERPAMYIGSTDTRGLHHLVYEVVD
                     NSIDEALAGYCSRISVIINKDGSLQVEDNGRGIPVDRMEKNGKSALEVVLTVLHAGGK
                     FDKESYQVSGGLHGVGVSVVNALSNWLSAHVFRDGNIYEMRFSQGKMTSPLSQREESL
                     NELLTRYQQWYGAPAPFVRGATPVDTPSIESERESRIAFLAQLGAKMTGTRIHFVPDA
                     TIFETTTFDYDVLAHRMRELAFLNAGLTIIITDERSSDTATFCYAGGLIEFVKYLNDG
                     LECLHPTPIDISKKDLENKLEIEVAMQYTTAYDEKIYSFVNSVNTKDGGTHLEGFRSA
                     ITRTINTVAKRNGLIKENSTITLRGEDVREGLTSVVSIKMANPQFEGQTKMRLGNSSV
                     KGTVDSLVYAALSEYFDENPTVLKIIIEKALSAAKAREAARNARDLARRKSSLESSGL
                     PGKLADCSERDPAKSEVYIVEGDSAGGSAKQGRDRKFQAILPLRGKILNVEKAGEHQI
                     LKNAEIQTLISAIGTGIGEKFDQERARYHHIVIMTDADVDGAHIRTLLLTFFYRYMLK
                     LIEAGYIYIAQPPLFRISKGKEEKWVYTEDEMKTASAAMGEKGVSVQRYKGLGEMNAQ
                     QLWDTTMDPEYRVFRQVTIEDALEADEMFRTLMGKDVEIRKNFITRHAKEVTNLDI"
     gene            complement(8459..8767)
                     /locus_tag="VFC43_04110"
     CDS             complement(8459..8767)
                     /locus_tag="VFC43_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30404.1"
                     /translation="MEYPFGHGFFTSIMLIIRHFALPPEQAWFGAGDHVEGLVLPEKF
                     KGTDVEEITMLLKKKVIWHQPGSMDKEEARDVVNTLNRLVVAIDRELGIADASIGDFR
                     "
     gene            complement(8806..9633)
                     /gene="cbiQ"
                     /locus_tag="VFC43_04115"
     CDS             complement(8806..9633)
                     /gene="cbiQ"
                     /locus_tag="VFC43_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HZK30405.1"
                     /translation="MIEELFYIEKSAYRDSYIHQLDARVKIISLFAIIITMVAIPYTP
                     TVFIIGLLFFVFLALQWVLAGLPWQVYAKRLVLVLPFGLFISLFQIFFTNRYYTVFHV
                     IADLPYGIHIYAESVEFAIILLVKFIICVSAIILLSSTTKLHDMLEGAGRVGLPPEFA
                     LTFGMMIRYLFVFGYMYRKINESLVTRCFDPFNPALPYRYRMQQIGYTVGTIFIRSYE
                     QGERVYTSMLCRGYGKNSHIFISKKPLQRSEWAFLSFSLTLTFAIPLVIQLNAVRLM"
     gene            complement(9630..10481)
                     /locus_tag="VFC43_04120"
     CDS             complement(9630..10481)
                     /locus_tag="VFC43_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZK30406.1"
                     /translation="MHLIETRNLTYTYPGNVTALHSLNFVAPRNARIAVIGSNGAGKS
                     TLFKCFNGIFKPSSGSVLIRGEPITKENIREVRKFVGIVFQNADDQIFSPTVEQDVAF
                     GPTNLGLDEETIHHRVHEALKIVGIEDLAHRVPHHLSGGEKKRVAIAGIIAMEPEVLV
                     LDEPTAGLDPKGVQDLVGFINSLSEKYGMTVIFSTHDVSMVAEVADYVYVMSRGKFVA
                     EGSVEEIFLQPDMLRSVRLDVPVLPKLIRSLNKKGIPVPMAYTYDDAERALAETYRGI
                     IKERYSP"
     gene            complement(10526..11527)
                     /gene="cbiM"
                     /locus_tag="VFC43_04125"
     CDS             complement(10526..11527)
                     /gene="cbiM"
                     /locus_tag="VFC43_04125"
                     /inference="COORDINATES: protein motif:HMM:NF004902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transporter CbiM"
                     /protein_id="HZK30407.1"
                     /translation="MHIPDGFFPISQAAVYWIIALIFIGLSLRWAKNEMSEEKVPLVA
                     VLAAGIFAIQTLNMALPISLIPGGVSGHVVGAALAAIVLGSPFAAIFILTLVLVLQGI
                     FFGDGGITAMGANIINMGVLGGFFGFYTYKSMNNLFRNRFFATFIAGWVSLFIPAIVC
                     AAELAVAGTLPLAFGLVTMSIYHAVIGVIEGGITAFALTLIIQARPDIVSGEIPVGSR
                     RIKDLFIVIGIGIALSISIFAPYYASAYPDGLDSTFLAAYGAKDVNTVHIDDAKAVSA
                     EVVVAEKTGNTFSWQAPLPGYTLPGFDKPGEILAILFGVLVLLAFGFGISRFMARKN"
BASE COUNT         2796 a         3019 c         2949 g         3137 t
ORIGIN      
        1 cggtcttcag cgtgaccagt tgtgaggaca ggatatggtc catgtcctcc cgggaatgaa
       61 ccgaccatgg cgggagtttt gtcgcgatcc ttactctttg ccggtatccg tcagcaagtg
      121 cccgggcaag tattttttca cttttcccga aatgatatgc aggagcggta tcgacataat
      181 ttaccccctg gtcgatagcg tgtcgcaatt gccgtatcgc gcgttcttca tcgatactcc
      241 ctcctttttt caatggcagc cgcatataac cgaacccgag gatcgagagt tcatcaccgt
      301 ttttgggaac tgtacgataa agcataaggg acacattctt ttttttttat tgcctgatta
      361 gtcaggtgga ttggtactgg tgatatataa tttttcttaa attccctgtc gcgcagatga
      421 acgtgacagc gacgagggct actttattca ggattaccgg gtattggaaa aaagatccgg
      481 ccttcgacta tctcccgcca taggatctga catgcttcca ggcccggtga tatttatcaa
      541 acgctccatt ttcgtcagtg tagatgtgcg ggtcttcacg gaggtaaagt ttcctgtcct
      601 ctccgaccgg acagactttg atacacagac cacagggtga aatgaaccgt ttattcaggg
      661 cttcagaccg ggtagcacag gatttcttgt cagtcagccc ttcgggatat tgtcgtccat
      721 cgagggcttt aaccgggcag acgtttacac attgcatgca cgatgtgcac aaagctttct
      781 gaataacagg atccggcggg atttccgctg cggtgaaaat cgaagtaaag cgcactctcg
      841 gtccgaattt ttcagtcaga agcatattgt ttataccaaa atttcccaga cctgcgaggt
      901 atgcagcgtg gcggtgagag aaaaatgcga gtggtttttc cttaaggatg cttattgatc
      961 cgtaaccgtc ccgggggatc cagactgagg ggaacccttc agtattgagg aacagggaga
     1021 tccggtaccc gtttatatcc agcagggtat ttaccgtacg gtacagttca tgataataga
     1081 tcgaaggtgc ggtttccaca atgggaagac tgacaggcag accgatgaca atgaccgtta
     1141 cggtttccgg gaaaaccgac cgtggccgga attccagcgg gatccatgga tcaaacagcg
     1201 ggtcgtccca tcttactgcc ggggcgaaac ccacgagcgg aatatcttgt tccgcacatt
     1261 ttgtgatgat tttttgtttc aaggccatgc tcatgatcta gcatagtatg attggcaata
     1321 aatggatttt gtgacattcc gtgccgtgga gagcacctgt tttgcgtcag acctccccaa
     1381 gcagagtgct atgatatatt taatacgctc aacgacaaga gaaaatcggg tgagcacata
     1441 actatggctt ttgttccgga tcccgcagtc ctggtaaatc ttctcctgtg tgtaataatt
     1501 ctcgcgctgg gatacctggt atatcggaaa cggaatagta tcagcgccct gtttattgct
     1561 gttgcatttg gtatgttcgg gttatctcac ctgagcacgc tgctcgcgca gacgttgttt
     1621 cctgacgtta cctttgtcct cctgagaatt tgtggatata tattcgttgc tgcggcactc
     1681 tacctgtcac tgacagaata accagcccgg ccaaaaagaa aagggaagcg tgccaaaacc
     1741 tctacaaaac tcaatcaggt cctataaccg tacatacgat tgtccgctca cgtcccgcgt
     1801 ttacatcaaa ttcaaggagg acaatctgct gccatgtccc gcacatcagg atacccccct
     1861 ttaaagggat ggtgagtgaa ggtccgacca gagctgcttt tacatgtgag cggccgttac
     1921 cgtcacccca gcgggcatta tgagcataat ctgcagtatc cggggcgaaa accgtgagtg
     1981 cccgtctgag atcagaaagt acacccggtt cgaactcaat tgtggtcagt gccgcagtag
     2041 aatgctggac aaaaaggtgg accaggccgt ttttgatacc gctctcgctg actgcttttc
     2101 tcactttctc cgtaagatcg atgatatcgc cttcgcactg cgatgtcact gttatggttt
     2161 ttatcaacat gaatacatcc ccgaagaata tctacaccgg gtaacaaaat catttcctgt
     2221 tgcagacagg tatggttgtg ctgagttgct gcaggaaata gccaaccgtt atacaccctg
     2281 agctccaaga ttatttttgc gtcagcgatg aaaaaaaata taccagcgga gcggggaaat
     2341 atcaacgacc tttcaccctt tcgtcaccgg tacgtggtcc ttgttatcgt tcttgcctct
     2401 gtgctgatgg ccgtcatcga cggcacagta gtcaacattg ccctgccatc aatgacccgg
     2461 tttttttctg ttgacctctc cgactcccaa tggacgatca ccgcatacct catcacgatg
     2521 acgagcctgc ttctcatttt tggcagggtg tcggagtatg tcgggcgtgc ccggctcttt
     2581 tttgtcggta taattatctt tactgtcagc tctcttgcct gtggactttc gaccggtctt
     2641 cctgaactca tcctgttccg ggttattcag ggtgccggtg ccgcgatgct tttttccatc
     2701 tcgtcagcac tcatcttcgc tacaacccct ccggcggaac gtgggcgtgc catgggatac
     2761 cttggtgcta cggtcgccat cgggagtatt gcgggaccga ttgtcggtgg ttttgtcgtg
     2821 gactcgcttg ggtggcagta tatttttttt atcaatatac ccctcggaat cctgcttatc
     2881 gctgcagcgg ctgcatacct gcggattgac gagaaaagga ctcattcgct ttcgcttgac
     2941 tggcacggtt caactgcaat ggtagcgatg tttgttgcct tgatccttac ccttggcagc
     3001 cttgctgaca tgggaatgat taccccgcca gctatcatct ccggtgtaat ttttttcctc
     3061 gcactgtccc tcttcatatg gcacgagcgc cgttgcccgg ctccccttct ggacctgaac
     3121 gtcttttcaa accgtgaatt tttattcccg gtcattgcgg ttgtgctggt ctttgtcgct
     3181 aatttcatga tggccgtggt cgggccattc tattttgaag gcgtactggg ataccgtccg
     3241 tcacaggttg ggctggtttt tttaatcaac cctgcagtta tggtgatagt agcccctctg
     3301 gccggatcgc tctatgatcg tcacccggcc cggaattatg cagcgctggg catgggtatc
     3361 acaacagtcg ccttcctgtt cctctcgtac tgcaccctta ctcagaatgt gtccgggatc
     3421 ctggttgcat tcgtgctctt cggaatcggt gtcggtcttt tccagtcccc caataacacc
     3481 gcaattttaa gtgctcttcc aaaagagcag ctctcaactg cttcaagcgt gattgcgacc
     3541 agcaggaatt tggggatggc tcttggggta tcgctgggca gtatcctgct ctcgttccag
     3601 ctcatgggtg caggatttac cggggatgta attactgcgg acccggcact tctggcagtc
     3661 tcgatcagcc ggataatggc ggtatcagca gtcctgtgtg ggatcgttgt tcttctctca
     3721 tcgctgaaac gataacacac aggtcggcta tactaaaaaa ccgaagagtt gaaaaaatag
     3781 tgattatccg gatttagtat tattcgttcg tttcagggtt agatgaatct gcacccgcat
     3841 ccgcaccgtt ctcgctgtcc acaacttcag gctggacaat tgctacacca acaacccggt
     3901 ctttgtcatc aagacgaatg gtcctgaccc cttttgtccc gcgcccgatg atccgtatct
     3961 cactgaccgg catacggatc acaactccgg atgcggtcat gacgataatc tcatcggtat
     4021 ccagtactgc acgcgattcg atgacgattg cgttccgttc gagctgcatg ttcctgacac
     4081 ccatcgtgcc ccggccatga ccgcggaagt cgtcaaattc cgtcctcttg ccaaaaccaa
     4141 catcggatat ggtgaggagg tgatcttttt caactaaggt aagtgcaacg atggtatcct
     4201 cgccccggag tttcattccc cgcactccgg aggcattgcg cataaccgta cgaatggtct
     4261 cttcatggaa ccggaggctg tagccaaacc gggtggacaa gacaatctct ttgcttccat
     4321 cggttgcaat gacatcgacc agctggtcgt tctctttgag ccggatggcg ttggttccca
     4381 cagaacgcgg gtttgaaaat tccgtctggg ggatcttgat gacctgcccg taccttgtcg
     4441 caaacagcat aaaccggtct tccctgaaaa cccggatcgg gatgaccttt gttacgatct
     4501 cgtcttttaa gttgaggagg ttgacgatag gtttgccttt tgcaatgcgg gaactttcgg
     4561 ggatctggta gactttaagc cagtagacac gaccgatatt ggtaaagcag agcaggtagt
     4621 ctttcatctc ggcggcaaat acccggtcca ccccatcatc gtctttggtg gtcattcccg
     4681 tgatcccgtg gccgcccctc ctctgtttgc ggtaggtatc gatatccatc cgcttgatat
     4741 aattggcagt agtaagcgag atcaggaccg tcttgtcctc gatcaggtcc tcattggaga
     4801 gatcactggt atcgattgcg atctcggttc tccgggcatc accgaatttt gctgcgattt
     4861 ccacagtctc gcgccggatc tcgtctttga tgttggcttc gttcgagagg atggtctcga
     4921 gccttccaat ctcggcttca agacccttct tctcgtcgtt tattttctgc tgctcgagcg
     4981 ctgcgagacg gcggagctgc atctggagga tcgcatttgc ctgcagttca tcaaggccga
     5041 acttggtaat taatgccacg cgggcgttat caaccgaatc ggatgcccgt atcgccgcga
     5101 tgattaagtc gattttggaa agggccaaga gcaggccgac taaaatatgg actttctcct
     5161 gggccttttt caggtcaaac tccgacctgc gccgtattac ttcgatccgg tggtggacaa
     5221 agttctctaa aagtccatga agggtgagca ccctgggctg gttgtcgaca atcgcgaggt
     5281 tgatcgccca gaaactggat tcgagcgcgg tcagcttgta gagctggttt aagatgactg
     5341 ccgacatcgt tcccttacgg agctcaaaga cgacccggat gccatccttg tcagactcat
     5401 cgcggatgtc ggaaagcccg tctatccttt tatccttgac catttcggca attgcggtta
     5461 tccattgcgc cttgttgacc tgatagggca gctcggaaac tatgatgcgg tcgccccggt
     5521 tgcctccttc gctctcttca atagtggcaa cccccctgac gataacgcga ccctgcccgg
     5581 tggtgtagat gttcctgacc ccttcggtcc ccatcatgac accgcctgtc gggaagtcgg
     5641 ggccgggcat gaccttcatg agatcttcaa ccggtatttc cgggttgtcg aggaagagtt
     5701 tgaccgctgc gcagacctcc cgcaggttgt gcgggggcat cttggtcgcc ataccgacag
     5761 caataccatc cgtgccgttg accagcaggt tgggaatctt tgacgggagg acggtaggct
     5821 ctttcaacga ctcatcgtag ttcgggatga accttaccgt atccttttca agatcctcca
     5881 ggagtgcttc tgcgtacggg aaaagcctga cctcggtata cctgctcgct gccgcagaat
     5941 caccgtcaac cgatccgaag ttcccctgtc cctgtaccag catattgcgg tacgagaacg
     6001 gctgtgccat cttgacgatc gtatcgtaga tggatgcatc gccgtgaggg tggtacttac
     6061 ccatcacgtc tccaaccacc ctcgcactct tcttggtggg cttgtcgctg gtgttgccca
     6121 tctcccacat cgcaaagagc gagcggcggt gaacgggttt gagaccatcg cggacatcgg
     6181 ggattgcacg cccgatgatg acactcatgg cgtaattgat gtacgaggtc ttcatctcct
     6241 gagcgatact gacaggttcg gtgcgatggg tttccgggac gcctgcggga ggtacttcag
     6301 atgtcaaggt tggtcacctc ctttgcatgg cgggtaatga aatttttcct gatttctaca
     6361 tcttttccca tcagcgtcct gaacatctcg tctgcctcta aggcatcctc aatagtgacc
     6421 tgtctgaaga cccggtactc cgggtccatg gtggtatccc agagctgctg ggcgttcatt
     6481 tcaccaagac ccttgtaacg ctggacagac acgccttttt cgcccatggc agccgaggcg
     6541 gtcttcatct catcttccgt gtacacccac ttctcttcct ttcctttcga gatacggaac
     6601 aggggtggct gggcgatata gatataaccg gcttctatca gtttgagcat gtaacggtag
     6661 aagaaggtca gcaggagggt gcggatatgg gctccgtcca catcagcatc ggtcatgatc
     6721 acgatgtggt ggtagcgggc ccgttcctgg tcgaatttct caccgatacc agtgccgatt
     6781 gcagagatga gcgtctggat ttcagcgttc tttaagatct ggtgttcacc ggctttttcc
     6841 acgttgagga tctttcctct gagggggagg atggcctgga acttgcggtc ccgtccctgc
     6901 ttggctgaac cgcctgctga gtcgccttcc acaatataga cctcgctctt tgccgggtcg
     6961 cgttccgagc agtccgcgag tttgccgggg agaccggaac tctcgagcga gctcttgcgc
     7021 cgtgcaaggt cgcgggcatt tcttgcagct tcgcgggcct ttgcagccga gagggctttt
     7081 tcaatgatga tcttgagtac agtcgggttc tcatcgaaat attcactcag tgctgcatag
     7141 accagcgagt cgacggtgcc cttgacgctg ctgttgccaa gacgcatctt ggtctgtcct
     7201 tcgaactggg ggttcgccat ctttatggag actacggagg tgaggccttc ccggacatct
     7261 tcgccccgga gggtgatggt cgagttctcc ttgataaggc cgttcctctt tgcaaccgtg
     7321 ttgatcgtcc tcgtaatggc gctcctgaac ccttcgagat gtgtcccgcc atccttggta
     7381 ttaaccgagt tgacgaacga gtagatcttt tcgtcatagg cagtagtata ctgcattgcc
     7441 acttcgatct cgagcttgtt ctcaaggtcc tttttggaga tatcaatggg ggttgggtgg
     7501 agacactcca gcccgtcgtt taagtatttg acaaattcga tgagtccgcc ggcataacaa
     7561 aacgttgccg tatcactgga ccgttcatcc gtgatgatga tcgtaaggcc tgcgttgaga
     7621 aacgcaagtt ctctcatccg gtgtgccagc acgtcataat cgaacgttgt ggtctcaaag
     7681 atggtggcat cgggaacaaa atggatacgt gtgccggtca tttttgcacc taactgtgca
     7741 aggaatgcta tgcggctctc ccgttcagac tcgattgaag gggtgtcgac aggtgttgcc
     7801 ccccggacga agggtgccgg tgccccgtac cactgctggt accgggtgag cagttcgttg
     7861 agtgattctt cacgctggga gagcggcgag gtcattttac cctgggaaaa ccgcatctca
     7921 tagatgttac cgtcacggaa tacgtgagcg gagagccagt tggagagtgc gttgacaaca
     7981 gaaacaccga cgccatggag acctcctgaa acctggtaac tctccttgtc gaacttacca
     8041 cctgcatgga gaacggtcag aaccacttca agggcactct ttccgttctt ctccatccgg
     8101 tcgaccggaa taccccgtcc attgtcttcc acctggagtg agccatcttt attgattatt
     8161 actgaaatcc ggctgcaata tccggcaagt gcctcatcga tagaattgtc cacaacttcg
     8221 tagacaaggt ggtgaagacc gcgggtatcg gtactgccga tatacattgc tggacgttca
     8281 cggaccgggg tgagtccttc gagaacggta atatgggatg catcgtatgt ttcagtcaag
     8341 gaaaacctcc gttaaaaaaa caataaaata cggtaaagaa atcacaatat ttattggtcg
     8401 tttgatgact taaaggatag gcgtaaaaac gggtttcggg tataaaaatg attctccttt
     8461 accggaaatc cccgattgat gcgtcagcta taccgagttc ccggtcaatc gctacaacca
     8521 gcctgttgag cgtattaact acatcccggg cctcttcctt gtccatacta cccggctggt
     8581 gccagataac tttctttttg agcagcatgg tgatctcctc gacatcggta cctttgaact
     8641 tttcgggcag taccagaccc tctacatgat ccccggcacc gaaccaggct tgttcgggag
     8701 gcagtgcaaa gtgccggatt atgagcatta tgctcgtgaa aaatccatga ccaaacggat
     8761 actccatgat aacaccatag tgataagtgg taaaaaaggt aatgtctaca taagccggac
     8821 tgcattcagc tggatcacaa ggggtattgc gaatgtaagg gtgagggaaa aggaaagaaa
     8881 tgcccattct gaacgctgca gtggtttttt ggagataaag atatggctgt ttttaccgta
     8941 tccgcggcac agcatacttg tatacacccg ttctccctgc tcatacgatc ggataaagat
     9001 tgttccgact gtgtacccga tctgttgcat acggtaacgg taaggaagtg ccggattgaa
     9061 cggatcaaaa caccgcgtga ccagggattc attgatcttc cggtacatat aaccaaagac
     9121 aaagagataa cggatcatca tcccgaaggt gagtgcaaac tcgggaggaa gccccacgcg
     9181 cccggctccc tcaagcatat catgaagttt cgttgttgaa gaaagcagga taatcgctga
     9241 gacgcagata ataaatttta ccagcaggat tatggcaaat tccacagact ctgcatagat
     9301 atggatacca tacggcagat ccgcaatgac atgaaacacc gtgtaatacc ggttggtgaa
     9361 aaatatctgg aagaggctga tgaaaagtcc aaacggtaat accaggacaa gacgctttgc
     9421 atagacctgc catggcagac cagccagaac ccactgcagg gcaaggaata cgaaaaatag
     9481 taaccctatg ataaaaactg tcggagtata gggaattgcg accatggtga tgattattgc
     9541 gaatagagaa atgattttta cccgtgcgtc gagctggtgg atataactat ccctgtacgc
     9601 tgatttctcg atgtaaaaga gttcctcgat catggggagt atcgttcctt aataattccc
     9661 ctgtaggtct ccgccagcgc ccgttcagca tcatcatagg tatatgccat ggggaccggg
     9721 atgccttttt tgttcaatga ccgtataagt tttggcaata ccggaacatc aagtcggact
     9781 gaccggagca tatccggctg taaaaatatt tcttcaacgc tcccctccgc gacaaatttt
     9841 ccccggctca tgacatacac gtagtcagcc acttctgcaa ccatcgatac atcatgagtt
     9901 gagaagatta cagtcatccc gtatttttca gagagtgaat ttatgaaccc gaccagatcc
     9961 tgaacaccct ttggatcaag cccggctgtc ggttcatcca gaaccagcac ttccggttcc
    10021 attgcaatga taccggctat tgcaacccgt ttcttctccc ccccgctcaa atgatggggg
    10081 acccggtgcg caaggtcttc gatgccaacg attttcagcg cttcatgaac ccggtgatga
    10141 attgtctctt cgtccagtcc gagattggta ggaccaaaag caacatcctg ctctaccgtg
    10201 ggtgaaaaga tctggtcatc cgcattctga aatacaatcc cgacaaactt gcgcacctca
    10261 cggatatttt ctttggtgat tggttccccg cgtatgagaa ctgaccccga agatggtttg
    10321 aatataccat tgaaacattt gaaaagcgta ctcttgcctg cgccattcga tccaattacg
    10381 gcaattcggg catttcgggg ggctacgaag ttcagggaat ggagtgctgt aacattgccg
    10441 ggataggtgt acgtcagatt gcgtgtttct atgaggtgca tgagaaaatc cgttaatagg
    10501 tggtcttttt tataaaaaaa tcctctcaat tcttccgtgc cataaaacgg ctgataccga
    10561 agccaaaagc caacaggaca agcacaccaa agagtattgc aagaatctct cctggtttat
    10621 cgaatccggg cagagtgtag ccaggaagtg gtgcctgcca ggagaaggta tttccggttt
    10681 tttctgccac tacaacttct gcagatacag cttttgcatc atcaatgtgc acggtattca
    10741 catcctttgc accgtatgca gccagaaacg tactatcaag cccgtcagga tatgcagacg
    10801 cataataagg agcaaaaatt gagatcgaga gagcgatgcc aattccaata acgataaaca
    10861 ggtcttttat cctgcgggaa cccacaggaa tctcgccgct tacaatatcc ggccttgcct
    10921 gaatgatgag ggtaagtgca aaagcggtga ttccaccttc gattactccg atgaccgcgt
    10981 gataaatgct catggtgaca agaccaaacg ccagagggag ggtacctgca acggcaagtt
    11041 cggctgcaca gacaattgcc ggaataaaga gcgaaaccca accggcaatg aatgtggcga
    11101 aaaaccggtt gcggaataga ttattcattg atttgtacgt ataaaaaccg aaaaaccctc
    11161 ccagcacacc catgttaatt atatttgccc ccatggcagt gataccccca tcaccaaaga
    11221 agatcccctg cagtaccagc acgagcgtga ggataaagat tgccgcaaat ggtgatccaa
    11281 gaacgattgc agcgagcgct gcaccgacaa catgaccgct tacaccacca ggaatcaggg
    11341 aaatgggcag cgccatgtta agggtctgga tggcaaatat gccggcggcc aggaccgcaa
    11401 caaggggcac tttctcctcg ctcatctcat tttttgccca acggagtgag agcccaatga
    11461 aaataagggc aataatccag tatactgctg cctgggagat cgggaaaaag ccgtcgggaa
    11521 tatgcatgat gactccggat tagttattac ttttctaaat tattgtaaca ctcagtagtt
    11581 aaaaagtatt gttatttgat attttatcga acacattcca cattttatat gaattatcac
    11641 aatgaattac cttagatcat gtatttaaaa agcatgttac tgttgtggtt tttccctcat
    11701 aactatcgtg ttaaactttt aaattctatg catttcaaat ctgtccagtc ttatcacttc
    11761 atactcttcc gtactttttt caaaacctta aaaaacaatc cgcaatgatg tacacaacac
    11821 tgaggattgc attaccatgg actccgacct ttcccgtatt ggaatatctc ttcccagcaa
    11881 cctgcttgac aagtttgatg a
//
LOCUS       DASRLV010000101         2754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_116553,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000101 DASRLV010000000
VERSION     DASRLV010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116553"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VFC43_04130"
     CDS             <1..280
                     /locus_tag="VFC43_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK30408.1"
                     /translation="EQTVDGIDLIVEDDGPGIPEKEKENIFRRKYFKNTGFGLFLSRE
                     ILSITSLTIRENGIPGEGAHFVIHAPKGTYREVMATTASPVLKIPGQM"
     gene            complement(446..1441)
                     /gene="hypE"
                     /locus_tag="VFC43_04135"
     CDS             complement(446..1441)
                     /gene="hypE"
                     /locus_tag="VFC43_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HZK30409.1"
                     /translation="MKVNMMHGAGGEVMGELLQTLTRFRHNNAGGIGLEAMDDGAVIP
                     LNGQNLVFTTDSHVVRPIFFPGGDIGRIAVCGTINDLAMMGGRPIALSCGMIIEEGFE
                     IDDLARIVASMDDALGEAGANLVTGDTKVMEKGALDGIVINTSGIGIARQVVRDNGLV
                     PGDVIIVSGTLGDHGIAIMAHREGFDLGAQIKSDVAPLWGMMEKVLDAGSIHAMKDPT
                     RGGFASAINEMARKSGVCIRIREANLPIRKSVRSAAAMLGIDPLEVANEGKVVMGVPA
                     SCSEAILAALHAHTYGRDAAVVGTVTSGSHVIMDTLVGGERYIEPPIGDPVPRVC"
     gene            complement(1443..1781)
                     /locus_tag="VFC43_04140"
     CDS             complement(1443..1781)
                     /locus_tag="VFC43_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HZK30410.1"
                     /translation="MHEYSIAYDLYATTKKAALENHASFVKKIRVEVGKMAMVNPEQV
                     VFLFNTIKEDDPLFTTAVLECNEMAPQTRCPCGYTGNEIFVCPVCGALPEMVKGREIV
                     VTSVEIEVAD"
     gene            complement(1781..2005)
                     /locus_tag="VFC43_04145"
     CDS             complement(1781..2005)
                     /locus_tag="VFC43_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HZK30411.1"
                     /translation="MCVAIPAEVLEIKEGNVGVVDYGDLRQEVRLDLVDVKIGEFVLV
                     HVGFAIQKLSREEGLETRELFKQVYAAMED"
     gene            2174..2590
                     /locus_tag="VFC43_04150"
     CDS             2174..2590
                     /locus_tag="VFC43_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30412.1"
                     /translation="MNVKNIAVSGFLAGILILILMVVINVLVNAVIPTDMGRYGGMRD
                     SDDLVMILFFFYPFVVAFTAAILFDAVRSSLTGTPLEKGLLFGALLLTIMTIPSLYVM
                     ITSMTWPIDFYISTALWEIISFPVMGILFIKIWKVS"
     gene            complement(2648..2732)
                     /locus_tag="VFC43_04155"
     tRNA            complement(2648..2732)
                     /locus_tag="VFC43_04155"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2696..2698),aa:Ser,seq:tga)
BASE COUNT          709 a          717 c          596 g          732 t
ORIGIN      
        1 ggagcagact gtggacggta ttgaccttat cgtggaagat gatggtccgg gaataccgga
       61 aaaggaaaaa gagaacatct tccgaaggaa atatttcaaa aataccggtt tcggtctgtt
      121 tttaagcagg gagatcctct caatcaccag ccttactatc cgtgagaacg ggataccggg
      181 ggagggtgcc catttcgtaa tccatgcccc gaagggaacg tacagggaag ttatggcaac
      241 taccgcttct ccggtattaa aaataccggg tcagatgtag aaaatacccg gaataattga
      301 gcaggctttt actcctgcca ttcttctctc gtttggaact catcacaagg ccttttcagc
      361 cttttatgcg attacactta tatatatctg ccaggaaatc gtctaaaatc atccgaatct
      421 gaaaaatacc cgctttcaag tgtacttagc agacacgggg tacaggatca cctatgggcg
      481 gttcaatata ccgctcgcca ccaaccagtg tatccataat aacgtgggaa ccactcgtaa
      541 cggtcccgac aacagctgca tctctgccgt aggtatgggc gtgcagcgct gcaaggatag
      601 cctcagaaca agaagcagga actcccataa cgacctttcc ttcatttgcg acttcgagtg
      661 gatctattcc cagcatcgct gcggcgcttc ggacgctttt ccggatcggg agatttgctt
      721 cccggatccg gatacacaca ccgctctttc ttgccatctc attgattgca cttgcaaacc
      781 ctccacgcgt cgggtctttc atcgcatgaa tggaaccggc gtccagcacc ttttccatca
      841 tcccccagag tggcgcaaca tcggacttga tctgtgcacc gaggtcaaaa ccctcccggt
      901 gtgccataat cgctatccca tgatcaccaa gagttccgga tacgatgatc acatctccgg
      961 gaacaagacc attgtcacga acaacctgcc gggcgatacc aattccggaa gtattgataa
     1021 cgatgccgtc cagtgctcct ttctccataa ccttggtatc ccctgtcaca agatttgcac
     1081 ccgcttcacc gagtgcatca tccatggaag caacaatcct tgcgaggtca tcgatttcaa
     1141 atccttcttc tatgatcatt ccgcaggaaa gtgcgatcgg tcgacctccc atcattgcaa
     1201 gatcgtttat cgttccgcag actgcgatcc tgccgatatc gcctccggga aagaaaatgg
     1261 ggcggactac atgtgaatcc gtggtaaaaa cgaggttctg cccgtttaac ggaattactg
     1321 caccgtcatc cattgcctca agtccgattc cgccggcatt gttgtgcctg aatctggtaa
     1381 gtgtctgaag gagctccccc atcacttcac ccccggcacc atgcatcatg ttgaccttca
     1441 tatcaatcgg caacctcaat ttctacgctg gtaactacaa tctccctgcc tttgaccatt
     1501 tcgggaagcg caccgcaaac agggcagaca aaaatctcgt ttccggtata tccgcaaggg
     1561 cacctggttt gtggcgccat ctcattgcat tcgagcaccg ctgttgtaaa aaggggatca
     1621 tcttccttga tcgtattgaa gagaaaaaca acctgttccg gatttaccat ggccattttt
     1681 ccaacttcaa cacgtatctt tttgacaaaa cttgcgtgat tctcaagtgc tgccttttta
     1741 gtagttgcgt ataagtcata tgcaatggag tactcgtgca tcaatcctcc attgccgcat
     1801 agacctgctt gaacagttcc cgtgtctcca gtccctcctc tctggaaagc ttctggatag
     1861 cgaacccgac atggactaat acaaattcgc cgattttgac gtcaacaaga tcgagcctga
     1921 cttcctgtct gagatcgccg taatcgacaa ctcccacatt cccctctttt atctccagca
     1981 cttctgcagg aattgcaacg cacatcgatg atcctctgga atatccatct catacattgg
     2041 aggttcaacc acataaaaac agtgatattg cgctaaaaaa accaatcatg acgttttttg
     2101 catagaggtg tggtttttag tttcacagcc tgcattccca atattaataa aaatgcagct
     2161 tcgataaaac ggtatgaatg taaaaaatat tgctgtctcc ggatttctgg caggaattct
     2221 tatcctcatc cttatggttg taatcaatgt actggtgaat gcagttatcc cgacggatat
     2281 gggcaggtac ggtggaatga gagattcaga tgatctggtg atgatccttt tcttcttcta
     2341 cccgtttgtg gtagcattta ccgcagcaat tctttttgat gcggtcagga gttcccttac
     2401 cgggacaccg cttgaaaagg gactgctgtt cggcgcattg cttctgacca tcatgaccat
     2461 tcccagcctg tacgtgatga tcacgtcaat gacatggcca attgatttct acatcagcac
     2521 cgcactctgg gagataattt cattccccgt aatgggtatt ttgtttataa aaatctggaa
     2581 agtttcctga aagagagtgc tgtcatggat agaatatgca tcctttataa aaatcaggaa
     2641 aaaactacgc cggggaagag atttgaactc ttgaggtgca gggcaccagt agctttcaag
     2701 gctaccgcct tcccggacta gactaccccg gcaatgctcc atatgttagg tgtc
//
LOCUS       DASRLV010000102         7784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_767410,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000102 DASRLV010000000
VERSION     DASRLV010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 7784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7784
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767410"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VFC43_04160"
     CDS             <1..411
                     /locus_tag="VFC43_04160"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286638.1"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating.nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HZK30413.1"
                     /translation="GGDPHRMNLSDGILIKDNHLVLVPLLTAIHAAKKVSAYTKIEVE
                     VETSDDALSATKAGADIIMLDNMSVEKITRTIDLLKREGLRDHIIIEISGGINEGNLP
                     EYAALDVDVISLGALTHSVRNLSVNLEILPCNIT"
     gene            562..1155
                     /locus_tag="VFC43_04165"
     CDS             562..1155
                     /locus_tag="VFC43_04165"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZK30414.1"
                     /translation="MGNPETDAGNTESSIEGVPVESAVFEERRKLRIKGFISLLTDPG
                     LDSRWKAAEALGVEGDASAVQPLIDALKDPYVDVQWLAAKSLGQLGDPRAIEPLVEAL
                     KSDEKWLRIGASWGLGKLRASRAVDPLLLILKDKKKLVRKMAAWALGSIGDDRAIGAL
                     TETLSDEAGEVRDAAQQALDSIKNEKAKKNTDTASKV"
     gene            complement(1219..1761)
                     /locus_tag="VFC43_04170"
     CDS             complement(1219..1761)
                     /locus_tag="VFC43_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30415.1"
                     /translation="MAPPESSISITVFDDGGNVVGYMLNMLTLLTYESVGDLNKPCQY
                     SPMFGRQERVAILLLLGVAVVVISAHLALGTLGKQPFAHTFAENSADGELVSASGIVE
                     QISYTKNGGHMNVYLNTTTIFIPAQVAQDIILNNGDSISVYGIVQTYRGKKEIVISSK
                     KDIFVIPDNKEKNSLHKPDL"
     gene            1694..1939
                     /locus_tag="VFC43_04175"
     CDS             1694..1939
                     /locus_tag="VFC43_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HZK30416.1"
                     /translation="MYPTTLPPSSKTVMEILDSGGAMTHKDLVQKTHLAPRTVRYALK
                     KLKERHLIIEKFNFRDARQIIYQNKTEQTNKPQPACV"
     gene            2011..2244
                     /locus_tag="VFC43_04180"
     CDS             2011..2244
                     /locus_tag="VFC43_04180"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZK30417.1"
                     /translation="MVSRLVQRQGLSQSDAAKRLGVTRAAVSQYMSKKRGAGECDIST
                     ELDSLIDRWTLAVVTGESDINLCDVCQCALKKF"
     gene            complement(2320..3984)
                     /locus_tag="VFC43_04185"
     CDS             complement(2320..3984)
                     /locus_tag="VFC43_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HZK30418.1"
                     /translation="MHRFLAFVILLSCAWQTCAAVQIVEFCPDPHLYDDTDEYLVLSG
                     TGSLDGVIISDGKEGFRFPPGTKINGILTIARSGTAYKQSHGIWPDFEWLDYSPAIPN
                     VINGDPLRMANAKDVLMLYENGNLVQNVSWPEDCKPREGQIHYLEKGVWDPRPLMIGQ
                     SRFSPAVFHNVSVTTFVSPDCSNDMFSYAVNQASGEVLLNVYEFSSPAMADTLIAARD
                     RNVDVRIFLEGGPVGGITTEEKGVIQKMTERGIPVSVMESHNGIPAPYRYNHGKYVVI
                     DRKSLLITSENFKYSGFPPTGINGNRGWGVFLEDPGLAGYFSTVYITDSQGIFVNAYN
                     GSRGDLEPIPPRNHTGDFLPAYFEGATVYPVIAPDTSYQITALINSAQNSIEIEQAYI
                     TNDSPSRLNPYLSAAINASRRGVHVRVLLDSYWFNVEDEKDNDEMVEIINRIGRSENI
                     PLEARCAELTLIDVDKIHNKGVIVDNQFALVSSINWNSVSPNFNREAGVIIDHPGVAQ
                     YFLTVFEDDWKPSVKSQGTTTDYMKIIVVIAVVISLIVLYYYRRRE"
     gene            4041..4664
                     /locus_tag="VFC43_04190"
     CDS             4041..4664
                     /locus_tag="VFC43_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="uridylate kinase"
                     /protein_id="HZK30419.1"
                     /translation="MNPVLPSHDTKIALPLVVKVGGSLFNQIPTLAPILNSSKRPLFI
                     IPGGAHFADTVRQCHVDEDTAHWMAIAAMEQYGWFIASFGIPVTEKLALCQKTTVFLP
                     YHCLRRNDMLAHTWKITSDTIAAWVAATLGLDLLVLKSVDGIYINGILQPRVTRPVDT
                     DVVDPFFIPFVLKSSVKTTIINGTDPKLVENILKGAGVSGTEIGTTF"
     gene            4705..4869
                     /locus_tag="VFC43_04195"
     CDS             4705..4869
                     /locus_tag="VFC43_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HZK30420.1"
                     /translation="MMSDKKCTSCNAPLAEEGATEFGCPACGVEINRCYRCREQSIPY
                     TCPKCGFGGP"
     gene            4873..5130
                     /locus_tag="VFC43_04200"
     CDS             4873..5130
                     /locus_tag="VFC43_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 1-beta"
                     /protein_id="HZK30421.1"
                     /translation="MGSVAVIARVMPDSPEVNLDELKKALKEKLPGIQEIREEPIGFG
                     LKALKLVAIINDAGGETDAIEKSLSEVAGVERAEIIEVTLT"
     gene            complement(5156..6109)
                     /locus_tag="VFC43_04205"
     CDS             complement(5156..6109)
                     /locus_tag="VFC43_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HZK30422.1"
                     /translation="MSKVTIIGATGNVGMFASYAISANPHVREILLYGREGRETLLRG
                     ITQDFIDSFAARGTNIKISWTTNLKDAAGSDVVVITSGIPRTPGQDRLDLALGNAQVI
                     APLAQTIGVLAPDTKILVVTNPVDVMTCVALKYSGLKPGQVFGLGTHLDSMRLKSLIA
                     AYFKVHVSEVHTRIIGEHGDSMVPLWSATTIGGIKISNLPAFAHLPVKEIIESVKCSG
                     NLIIKDKGSTVYGPGEAIASLVKTILGDENRILTVSAYIKSEVHGIGEGDVCIGVPAR
                     INRNGVFPITIKIDESEVIAFRESVEKIRTITKKVLDNLDV"
     gene            complement(6121..6431)
                     /gene="ffs"
                     /locus_tag="VFC43_04210"
     ncRNA           complement(6121..6431)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFC43_04210"
                     /product="signal recognition particle sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01857"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal-type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01857"
     gene            complement(6517..7548)
                     /locus_tag="VFC43_04215"
     CDS             complement(6517..7548)
                     /locus_tag="VFC43_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30423.1"
                     /translation="MKCPVCDMECITPTKDLIATLPSIFLPCADCSMRILDKRSPLPV
                     LIYDQPCSCGKRFIDEVFAHMYVIMVEEGDLKQTDPLIAVGTPLVHPGFVMERPPFLS
                     EKSLVLLTSRATKRTAERLFLEVPEIRGVVKKDDFIPGITSPDLHTVPRVYELLAGCD
                     VRADVYPLRTGPLVTYKQQTLIHIEFPRPGYPKIRAVEQHVGQPPITYFVDACSGVGT
                     LGLSAACLGVHHVVINDAWYASAFWSAFNLEINRAYFNIDKVKIFEQLHDMQKHPVTR
                     DPIKIAETTGEQIIEVYQGDFHKLYTVLPPGGNPVTALDIFDKKNQAAILEVRNEWQK
                     RVGGEIFIP"
     gene            complement(7645..7719)
                     /locus_tag="VFC43_04220"
     tRNA            complement(7645..7719)
                     /locus_tag="VFC43_04220"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7682..7684),aa:Ile,seq:cat)
BASE COUNT         2134 a         1807 c         1803 g         2040 t
ORIGIN      
        1 gggggcgatc ctcatcgtat gaatctgagc gatggaatcc ttatcaaaga taatcatctg
       61 gtactggtac cccttcttac agcaatccac gctgcaaaaa aggtgtcagc atatacaaaa
      121 attgaagtag aagttgaaac atcagatgat gcgctgtctg ccactaaagc cggtgcagat
      181 attatcatgc ttgacaatat gagcgtagaa aagattaccc ggactattga cctcctgaaa
      241 cgagagggtt tgagggatca cattatcatt gaaatttccg gaggcatcaa tgaggggaat
      301 ttaccggagt atgcagcact cgatgtcgat gtgatcagtc ttggggcact gacccattca
      361 gtgagaaatt tatccgtaaa tcttgaaatt cttccctgta atatcacata aattttttta
      421 aaattcaaca atcgggatac aattattgtg aggcatggcg cgatgtggtc gccaattaaa
      481 atttcttaat tttttttagt gagttttttt gaaaaactta atagtccgat accacgtatg
      541 ggtggctatt gaggaactgg aatgggcaat ccagaaactg atgcaggcaa caccgaatct
      601 tccattgaag gagtccccgt tgagtcagcg gtatttgaag agcgccgaaa acttcgaata
      661 aaagggttta tctccctcct gacagatccg ggtctagaca gtcgctggaa ggcagcggaa
      721 gccctgggag tcgaagggga tgcttcagct gttcagccgc ttattgatgc actcaaagac
      781 ccttatgtcg atgtacagtg gcttgcagca aagtccctgg gacaattggg ggatccccga
      841 gcgatcgaac cgcttgtcga agcgctgaaa tccgatgaaa aatggctccg tattggagct
      901 tcatggggtc ttggaaaatt gcgggcttcc cgtgccgttg acccgcttct tctgattctt
      961 aaagataaaa agaaacttgt ccggaagatg gcggcatggg cccttggcag tattggcgat
     1021 gacagggcaa ttggagcgct tactgagaca ctttctgatg aggccgggga agtacgtgat
     1081 gcagcacagc aggcgctcga ttcaataaaa aatgaaaaag ccaaaaagaa taccgacact
     1141 gcttccaagg tttaaggaga ggatttcagg tacctttttg tgtctggtgc agaacaggtt
     1201 tttttggcat tttaatatct ataagtcagg tttatgcagg gaattttttt ccttattatc
     1261 aggaatgaca aagatatcct ttttggagct gatcacaatc tctttttttc cacgatacgt
     1321 ttgaacaata ccataaacag aaattgaatc cccgttgttg agaattatat cctgtgcaac
     1381 ctgcgcggga ataaaaatgg ttgtagtatt cagataaaca ttcatatgcc ccccattttt
     1441 tgtataacta atctgctcga ctattccact tgcagatacc agctcgccat ctgcagaatt
     1501 ttcagcaaat gtgtgggcaa agggctgttt tccgagggtc ccaagagcca ggtgcgcaga
     1561 gatcaccaca accgcgacac ccagtaaaag cagaattgca acacgttcct gtcgcccgaa
     1621 cattggtgag tattgacagg gtttgtttaa atcaccgaca gattcatatg ttaacagtgt
     1681 taacatattc aacatgtatc ctacaacatt acccccgtca tcaaagacgg taatggagat
     1741 actggattca gggggcgcca tgacccataa agatctcgtg caaaaaacac atcttgcacc
     1801 acgaacagtt cgttatgcac tgaagaagct caaagaacgg catctcatca tagagaagtt
     1861 caacttccgt gatgcgcgtc agattattta ccagaataaa accgaacaga cgaataaacc
     1921 ccagcctgcg tgtgtgtgat gaaacactgc tgcactctga tgcattgtga taccatggtc
     1981 cggaaccttc tcccacccat gagggcggaa atggtttcaa gactggttca gaggcagggg
     2041 ctaagtcaga gtgatgcagc caagcggctt ggagtaacaa gagccgctgt atcccagtac
     2101 atgagtaaaa aaagaggggc gggagaatgc gatatttcta cggagcttga ttcattgata
     2161 gaccgctgga cactcgccgt agtaaccggt gaaagtgaca taaatttatg tgatgtatgc
     2221 cagtgtgcac ttaagaagtt ctaaaaaaaa agaatcacca ttcttgtatc attcctgttt
     2281 gcataatttt acatagaaac cattcagagt cacaccacat cattcccgac gtctgtaata
     2341 atagagaaca atgagagaaa tgaccactgc aataactaca atgattttca tgtaatcggt
     2401 tgttgtcccc tgagatttaa ctgagggttt ccagtcgtct tcaaatactg taaggaagta
     2461 ctgtgcaacc cccggatggt caataattac tcctgcttcg cgattgaagt tgggcgatac
     2521 gctgttccag ttaatgctgc tcacgagtgc aaactggtta tccacgatca ctcctttatt
     2581 atggatctta tcaacatcga tcagcgttag ttctgcgcac ctggcttcaa gcggaatatt
     2641 ttcagatctg ccgatccggt tgataatttc gaccatttca tcattatctt tctcgtcttc
     2701 gacattgaac cagtaagaat caagcagcac cctcacatga accccacgcc gtgacgcatt
     2761 gattgctgcc gaaagatacg gattgaggcg ggaaggggaa tcattggtga tatatgcctg
     2821 ctctatctca atactattct gtgcggagtt tatgagagca gtaatctgat agctggtatc
     2881 cggagcgatt accggataaa ccgtcgcacc ctcaaaatat gctggcaaga aatctcctgt
     2941 atgattcctt ggaggaatcg gttccagatc ccctctcgat ccattatagg cattcacgaa
     3001 tatgccttga gaatcagtta tatagactgt tgagaaatac cctgcaagtc cgggatcttc
     3061 aagaaacact ccccagccac gatttccatt gattcctgtg gggggaaaac cgctgtattt
     3121 aaagttctca ctggtaatca gaagtgattt cctgtcgatt actacatatt ttccatggtt
     3181 gtagcgatag ggcgcaggga ttccattatg tgactccata acagatacgg gaatcccccg
     3241 ttcagtcatt ttctgtatca ctcctttttc ttcagtcgtt attcccccga ccggcccccc
     3301 ttcaagaaat attctcacat caacgttcct gtcgcgtgca gcaatcagcg tatctgccat
     3361 ggcaggactt gaaaattcat acacattcag gagcacttcg cctgaggcct ggttcaccgc
     3421 ataagaaaac atatcgttcg aacaatcagg agaaacaaat gttgttaccg aaacattatg
     3481 gaagactgcc ggcgagaagc gtgattgtcc tatcataagt gggcggggat cccatacgcc
     3541 tttttcgaga taatggatct ggccttcacg aggtttgcaa tcctcaggcc acgatacatt
     3601 ctggacgaga tttccgttct catacagcat gagaacatct ttcgcatttg ccatacggag
     3661 aggatcccca tttattacat tcggaattgc cggggaatag tcgagccatt caaaatccgg
     3721 ccatattcca tgactttgtt tatatgcagt cccgctgcgg gctatagtaa gaatcccgtt
     3781 aatctttgtc cccgggggaa accgaaatcc ttccttaccg tctgaaatga taaccccgtc
     3841 aagagagccg gtacctgaaa ggacgagata ttcatcggta tcatcataga gatgagggtc
     3901 tggacagaac tcaacaatct ggactgcggc gcaggtttgc caggcgcacg acaaaagaat
     3961 tacgaacgca aggaaccgat gcatgaacac tccaatgcgt catgctcctt ttataatctg
     4021 gcgatatact ttttgtaaca atgaaccctg tattgccatc gcacgatacc aaaatagcat
     4081 taccacttgt tgtaaaggtc gggggcagcc tttttaatca gattccaaca cttgcgccga
     4141 ttctgaattc gtcaaaacgc cctcttttta tcatcccggg cggggcacat tttgccgata
     4201 ctgtccggca gtgtcatgtc gatgaggata cagcccactg gatggcaatt gctgcaatgg
     4261 aacaatatgg ctggtttatt gcatcgtttg gtattccagt aaccgagaaa ctggcattat
     4321 gtcaaaaaac aacagttttt cttccgtacc attgcctcag aaggaatgac atgcttgccc
     4381 acacatggaa aattacttca gatactatcg ctgcatgggt ggcagccact cttggtcttg
     4441 atcttctcgt cctcaaatcc gtcgatggaa tatacatcaa cgggatcctc cagccacggg
     4501 taacacgacc ggtagatact gatgttgttg atcccttttt tattccgttc gttttaaaaa
     4561 gctcagtaaa aacaaccatc atcaatggaa cggatccaaa actcgtggaa aacattctga
     4621 agggagcggg tgtttcagga actgagatcg gtacaacctt ttaaaggttc taaaagagat
     4681 actataggaa tccttggagg agcaataatg tcagataaaa aatgtacgtc ctgcaatgcc
     4741 ccgcttgcag aagagggggc aactgaattc ggatgccccg catgtggtgt tgagattaac
     4801 cgttgttacc ggtgcaggga gcagagtata ccctatacct gcccaaaatg tggattcggg
     4861 ggaccgtaat acatgggcag tgttgcagtt attgcaaggg ttatgcccga ttcaccggaa
     4921 gtaaaccttg acgagctgaa aaaagcacta aaagagaaac ttcccggaat ccaggagatc
     4981 agggaagaac cgattggttt tggtttaaaa gctctgaaac tggtcgcgat cattaatgac
     5041 gcaggtggcg aaaccgatgc catcgaaaag tccctgagtg aagtagcggg tgttgaacgg
     5101 gcagagatta ttgaagtgac gttaacctaa aaggaggtta catcgtctct tttttctaaa
     5161 catcaagatt atcgagaacc tttttggtga ttgtccgtat tttttcaaca gattcccgga
     5221 atgctatcac ttccgattca tcgatcttga tagtgatggg aaataccccg tttcggttta
     5281 tccgggcagg tacgccaata cagacatcac cttccccgat accatgtacc tcgcttttta
     5341 tataggctga aacagtcaga atccggtttt catctcccag tattgtcttt actaatgaag
     5401 caattgcttc tcctggacca tacacagtag atcctttatc cttgataatc aggttcccac
     5461 tgcattttac tgattcaata atttctttaa ccgggaggtg tgcaaacgct ggcagattgg
     5521 atattttgat acccccgatt gttgtggctg accataacgg gaccatgctg tcaccgtgtt
     5581 ccccgataat ccgcgtgtga acttcactta catggacttt aaaataggct gcaatgagag
     5641 actttaagcg catcgaatca agatgcgtgc caagaccaaa aacctgacct ggcttaagac
     5701 cggaatattt cagggctaca caggtcatta cgtcaaccgg attggttacg acgagaatct
     5761 tggtgtctgg agcgaggacg ccaatagtct gtgcaagtgg tgcgatcacc tgtgcatttc
     5821 ccagtgcaag atcaagacga tcctgtccgg gcgttctggg aatccccgat gtaattacaa
     5881 caacatcgga tcctgcggcg tcttttaaat ttgttgtcca gctgattttg atatttgttc
     5941 cacgtgcagc aaaggaatcg ataaaatcct gggtgatacc acggagaagt gtttcgcgcc
     6001 cttcacgccc atagagaagg atttctcgta catggggatt tgcagatatg gcataggatg
     6061 caaacatgcc gacattgccg gtagcaccaa taatcgttac tttggacatg ataatcgaac
     6121 atggggacgt gttatcggtc gactgtacgt tactgcgctt gccaaagcct ccatcctccg
     6181 ccccgttacc ccatgggcgc cggatgtcta cggtaagtct gctcccttcc gggcctgaac
     6241 cagtccccgc agcaacaggt cgccattccc tccggaattt tgatacctgg ccatcgacct
     6301 catgggacga ggtttcacag tcagaacgca caagctcccg caggccgttg cagccttcgt
     6361 caaacttcgc cccccgcgta ccgacggttt caggtaacag gtgacgccga accacccggg
     6421 ctagtcccca tatacctttg tgcactggtc aataaaggct ttttacccgg ttcaggtttc
     6481 catgtgcgga ttattccttg ttcctccgac taaaactcag ggaatgaaaa tctccccgcc
     6541 aactcttttt tgccattcat ttctgacttc gagtatggca gcctgatttt ttttatcgaa
     6601 aatatcgagt gctgtaaccg gattcccccc aggaggtaat accgtatata atttatggaa
     6661 gtctccctga taaacctcga ttatctgttc accggtggtt tcggcaatct tgatgggatc
     6721 tctggtgacc ggatgtttct gcatgtcatg taactgttca aagatcttca ccttgtcgat
     6781 gttaaaataa gcccggttta tctccagatt gaaggccgac cagaatgctg atgcatacca
     6841 ggcatcattt atcacgacat ggtggacgcc aaggcatgct gcagaaagac cgagagttcc
     6901 cactcctgaa cacgcatcaa caaaataggt gatcggcggt tgcccgacat gctgttcaac
     6961 cgcgcgtatc ttggggtaac ccgggcgtgg gaactcgata tgaatgagtg tctgttgctt
     7021 ataggtgacc agcggacccg tacgaagggg ataaacatca gccctgacgt cgcatccggc
     7081 aagaagttca tacactcttg gaacggtatg gaggtcagga ctcgttatcc cgggaatgaa
     7141 atcatctttc ttcacaaccc cacggatttc gggcacctca agaaaaagac gttcggccgt
     7201 cctttttgtc gctcgtgaag tgagcagaac aagtgatttt tccgaaagaa aaggggggcg
     7261 ttccatgaca aatccgggat gcacaagagg ggttcctacc gcaataagcg ggtctgtctg
     7321 tttaagatcc ccctcctcaa ccatgatgac atacatatgg gcgaatactt catcgatgaa
     7381 gcgctttccg cacgaacatg gttgatcata gatgagtacc ggtaatggtg aacgcttgtc
     7441 aaggatccgc atactacaat ctgcacaggg aagaaaaatc gaggggagtg tggcgataag
     7501 atcttttgtg ggggtaatac attccatgtc gcatacggga cacttcatgt tgcttgggta
     7561 tgatttttca ggaatagata gtttttcaaa cgtgcgattg aaaacgaaat tctcaccata
     7621 aaaaaacacg gacaacaaaa agaatgggcc cgatgcgatt cgaacgcatg accgcccggt
     7681 tatgagccgg gcgctctaac cggactgagc tacgggccca aaaacgcccc ccacagggct
     7741 cgaacctgtg acattcggat taacagtccg acactctacc aact
//
LOCUS       DASRLV010000103         3198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_117555,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000103 DASRLV010000000
VERSION     DASRLV010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117555"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1215)
                     /locus_tag="VFC43_04225"
     CDS             complement(85..1215)
                     /locus_tag="VFC43_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZK30424.1"
                     /translation="MDGSFRIGRIFGIPILIHYTFLLVIPLFAWIIGSQIALTAGMIN
                     EIFLVPVDTTLLTDGYVPYLVGIIVALGLFAGVLVHELAHSLVARYKGIRINSITLMI
                     FGGIASMEEGVPDPKAELPMALVGPLTSLVVGLVCSGIVYAVPTVISNPASGGIWVFV
                     FGYLGLLNIILCAFNLLPAFPMDGGRVLRAWLATRMPLYRATKIAADIGKGFAIIFGI
                     VGLFSNPFLILIALFIYIGASSESTAVKYSFLLQNVTVGDMMSSPVTTVSPTQPLREV
                     ITMMYSSKHLGFPVVERDTLIGMITLADVNRTSPIDREAMQVRDIMTRDPVTLPPSAP
                     VIDALKIMSARNFGRIPVVQDGKILGIVTRTDIIKVTELKQI"
     gene            1267..1530
                     /locus_tag="VFC43_04230"
     CDS             1267..1530
                     /locus_tag="VFC43_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HZK30425.1"
                     /translation="METQITELFGLQIYTDKGMFVGEVEDVLIDVDQKKIDSIVVSKV
                     NDQLFELKNYKGLKIPYRIISAIDDIVLIRHLPGAFTSGTMEE"
     gene            1585..2337
                     /locus_tag="VFC43_04235"
     CDS             1585..2337
                     /locus_tag="VFC43_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008193 - tRNA guanylyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(His) guanylyltransferase Thg1 family
                     protein"
                     /protein_id="HZK30426.1"
                     /translation="MVVGMENREIFSTITTLPPVFVRLDGRAFHRLSENLSLKKPFDD
                     RFSDAMVRVCTGLLADSGLCPDFAFTFSDEISLYFAQLPFGGRVEKIDSVSSSFAASS
                     LTLALGTSTPLSFDARVIPATPEYAMEYLANRQNEAWRNHVNGYCQQVLIEEGMSAKK
                     AAEHLKGLPVKALHEMMHKRGFNLATTPAWQRRGVLVYKKITEKEGFNPITQQTVIAE
                     RSAVTVDRDLLMFTSPEGKKFMEKLVLSVKSD"
     gene            complement(2396..>3198)
                     /locus_tag="VFC43_04240"
     CDS             complement(2396..>3198)
                     /locus_tag="VFC43_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HZK30427.1"
                     /translation="LEHAVEIVRSFDDACANARQAGKRKLRWPVSEVVVVTGSETVKD
                     AIERLNAVCMDRANARKVSVVMGRWDRIGWQAEPVMKALGKGFGKNSFKVKGIIEAAD
                     GNHLKAETDAGRTVKLGSGSEVFDIGADHVTFTEKLPADIFSAPMPDATVYVDVRLDD
                     SLETEWYEREVIRRIQEMRKQLDLVVSEFISVEVLIGDTRVFDLLNNNRGENIAGEVL
                     AVVPGKGVLFTILKPGDTLRKHDSVKDWEIEGATETVSMTIGISRIRI"
BASE COUNT          830 a          823 c          786 g          759 t
ORIGIN      
        1 agtaatcgca aagcgcccct ttaaaacatt agaggaatac caaaagattg ccgggatatc
       61 cagcatcgat ccgctgctga aattctaaat ctgcttcagt tcggtcactt tgatgatatc
      121 ggtccgggta acaatcccca gaatctttcc atcctggaca accgggatcc tgccgaagtt
      181 acgggcagac atgatcttca gcgcatcaat caccggggct gaaggcggaa gtgtaaccgg
      241 atcacgggtc atgatgtccc ggacctgcat cgcctcccgg tctatcgggg atgtacggtt
      301 tacatcggcg agtgttatca tgccgatcag ggtatcacgc tctactacgg gaaacccgag
      361 gtgcttgctg gagtacatca tggtaatgac ctctcttaag ggctgggtgg gggagacggt
      421 agtaaccggg ctgctcatca tatcgccaac ggtcacattc tggagaagaa aactatactt
      481 caccgcggtg gattcactgc ttgccccgat atagatgaag agcgcgatca ggatcaggaa
      541 agggttggaa aaaagcccga cgataccaaa gataatggca aaccccttgc caatatccgc
      601 ggcaatcttt gttgcacggt agagcggcat tctggtggcc agccaagccc ggagcaccct
      661 gcccccgtcc atcgggaatg caggcaggag gttgaaggca cacaggatga tgttgagcaa
      721 accgagatac ccgaaaacaa agacccatat tccgccagaa gcaggatttg aaataaccgt
      781 ggggactgca tagacaatcc cgctgcagac gaggccaact acgaggctgg taaggggtcc
      841 gacaagggcc ataggcagtt cggctttcgg gtccggtact ccctcctcca tggatgcaat
      901 acccccgaat atcatgaggg tgatgctgtt gatacggatc cctttatagc gggcaaccag
      961 cgagtgtgcg agttcgtgca cgaggacccc ggcaaaaagg ccaagagcta caataatgcc
     1021 aaccagatac ggaacgtaac cgtcggtaag caaggtagtg tcaaccggta ccagaaagat
     1081 ctcattgatc attcctgcag taagtgcgat ctggctcccg attatccagg caaagagggg
     1141 aatgaccagg agaaaagtat agtggatcag gatcggaatc ccgaaaatgc ggccgatacg
     1201 aaaagacccg tccataacaa aggattatct ttttaactct taagtatata ctttcatctg
     1261 ataacgatgg aaacccagat tacggagttg ttcgggcttc agatctatac ggacaaaggc
     1321 atgtttgtcg gtgaagtcga agatgttctc attgacgtgg atcagaagaa gatagattca
     1381 attgtcgtca gcaaggtcaa cgaccagctc ttcgagctga aaaattacaa gggcctcaaa
     1441 atcccgtacc gcatcatcag cgccatcgat gatattgtgc tgatccgcca cctgcccggc
     1501 gccttcacca gcggcaccat ggaagagtaa gcaggtatac attttgctgc gggacaaaat
     1561 tttcatattt ttatgtgctc ccgcatggtt gtaggtatgg aaaaccggga aatattttca
     1621 actatcacta cccttcctcc ggtgtttgtc aggcttgacg ggcgtgcgtt tcaccggctt
     1681 tcggaaaatc tttcattaaa aaaaccgttt gatgaccggt tcagtgatgc gatggtccgg
     1741 gtctgtacag gacttctggc agacagcggg ctctgtcctg attttgcgtt cacattctca
     1801 gatgagatca gcctctattt tgcccaactg ccatttggcg gccgggtgga aaagatcgat
     1861 tcggtctcat catcttttgc tgcaagttca ctcactcttg cccttggaac ctcaactccc
     1921 ctgtcgtttg acgcacgggt cattccggca acccccgaat atgccatgga atatctggcc
     1981 aaccggcaga acgaagcatg gcgtaaccac gtaaacggct attgccagca agtgttgatt
     2041 gaagagggaa tgagtgcaaa aaaagccgcc gaacatctaa aaggcctgcc ggtaaaagcc
     2101 ctccatgaaa tgatgcataa acgcggtttt aaccttgcta caactccggc atggcagcgg
     2161 cggggtgttc tggtatacaa gaagataaca gaaaaggaag gcttcaaccc gatcacacaa
     2221 cagaccgtga tagcagagcg gagcgcagtt accgttgatc gggatcttct gatgtttaca
     2281 agtcccgaag gaaaaaagtt tatggaaaaa ttagttctgt ccgtgaaatc ggattaaaca
     2341 ctttgatcag ttctgccttt accttcatga aatgagaaga ggattgttaa ataatttaaa
     2401 tcctgatacg ggatatcccg atagtcatgc tgacggtctc tgttgctcct tctatttccc
     2461 agtccttgac tgagtcatgt ttcctgagcg tatcgccagg tttgaggatg gtaaacagga
     2521 ctccttttcc cggaacaact gccaaaacct cacctgcaat gttctctccg cggttattat
     2581 ttaaaagatc gaaaactctt gtatccccga tcagcacttc caccgagatg aactcgctga
     2641 cgacaaggtc gagttgcttg cgcatttcct ggatacggcg gatgacctca cgctcatacc
     2701 attctgtttc gaggctgtca tcaagcctga catccacata cacggttgcg tccggcatcg
     2761 gggcggagaa gatatcagcc ggcagcttct ccgtgaaagt aacatgatct gctccaatat
     2821 caaagacctc gctgccagaa ccgagtttta cggtccgccc tgcatctgtc tctgccttga
     2881 ggtggttccc gtcagcagcc tcgatgatcc ctttgacttt aaacgagttt ttgccaaatc
     2941 ctttcccgag cgccttcatc accggctccg cctgccagcc gatacggtcc catcttccca
     3001 taacgactga gactttccgg gcgtttgccc ggtccatgca gacggcattc agccgttcaa
     3061 ttgcatcttt aaccgtttct gaacccgtaa ccactaccac ttcagatacc ggccagcgga
     3121 gtttccgctt tcctgcctgt cgggcatttg cacatgcatc atcaaacgac cgtacaattt
     3181 caacggcatg ttcgagag
//
LOCUS       DASRLV010000104         2913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1006360,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000104 DASRLV010000000
VERSION     DASRLV010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006360"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..111
                     /locus_tag="VFC43_04245"
     tRNA            38..111
                     /locus_tag="VFC43_04245"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72..74,aa:Phe,seq:gaa)
     gene            386..2281
                     /locus_tag="VFC43_04250"
     CDS             386..2281
                     /locus_tag="VFC43_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HZK30428.1"
                     /translation="MTGEYERAFHESVTRPERFWGAAAENIQWFQTYKRVLNNENPPF
                     YRWFEGGIVNSCYNALDYHIENGLKDRLALIYDSPVTKTKKRYTYGDLRDLVARCAGG
                     LRQVGVEKGDRVIIYMPMIPEAVIAMLACARIGAIHSVVFGGFAARELAARIEDAQPR
                     VILTASCGIEVNRIIPYQPLLESAIDMCSFKPSVCVIVQRPEAPAMLRNGKDISWDTL
                     MNADPVACVPLLATDPLYLLYTSGTTGKPKGVVRDNGGHLVALKWSMKNIYGVRPGEV
                     YWAASDIGWVVGHSYIVYGPLFYGCTTILYEGKSVGTPDAGAFWRVISEHGVSVLFCA
                     PTAFRAIRKEDPEGRLIHNYDLTRFRMLFLAGERCDPDTLVWAQQQLKVPVIDHWWQT
                     ETGWAIGANCVGLELLPVKPGSCTRPVPGFDICVLDAKGEKVPAGETGNIVVRQPLPP
                     GCFMTLWQNDKDFVKTYFSAYPGYYLTSDAGYIDTEGYLWIMGRTDDIINIAGHRLST
                     GAMEEVLASHPDVAECAVAGVHDPVKGEVPLGFVVIKAGVTKLPDAISSELISLVRQK
                     IGPIASFKAAAVVKRLPKTRSGKILRGTIKKIADGVPYTVPATIDDPVILDEITLTLR
                     KMGYPKT"
BASE COUNT          755 a          673 c          731 g          754 t
ORIGIN      
        1 tcatttatta gtcaccagtg acaacaattg tactcctgcc ctagtagctc agcttgggag
       61 agcgctagac tgaagatcta gttgtcgcct gttcaaatca ggcctggggc actcttttgc
      121 tgcttttttg gaatttatca gaaaaacaga aaataaaatg gcttttacaa tttcccgaag
      181 attagagaat tcaatgaatg cacattcttt attatttcct ccatggtttc cctggtgtaa
      241 tgaggtttca tagggtgatg caccacattt gaactattaa cctgatgagt tgcaccactt
      301 atcattcatg ttggtgcgtt tttttggaaa agaataagac ctcctgtccc aatctttagt
      361 atatacgtca agggagaagt ggtgcatgac cggggagtat gaacgggcgt ttcatgaatc
      421 tgtaacacga ccggaacgct tctggggagc tgctgcagaa aatatccaat ggttccagac
      481 ctataaacgc gtgctcaaca atgaaaaccc tcctttctac cgctggtttg aagggggaat
      541 tgtcaattcc tgttacaatg ccctggatta tcatatagag aacgggttga aagaccgtct
      601 tgcattaatt tacgacagcc cggttacaaa aacaaaaaaa cgctatacct atggagacct
      661 gcgtgatctc gtggcacggt gcgccggggg gctgagacag gtcggggttg aaaagggcga
      721 ccgtgttatc atttatatgc ccatgattcc cgaagccgtg attgcgatgc tggcctgtgc
      781 acggatcggt gcgattcact cagttgtttt tgggggtttt gccgctcggg aacttgccgc
      841 ccgtatcgag gatgctcaac cccgtgtaat actaaccgct tcatgcggga ttgaggtcaa
      901 ccgcatcata ccctaccagc ctctcctgga atccgcaatc gacatgtgta gcttcaaacc
      961 gtcagtctgc gtaatcgtcc agcggccgga agcaccggct atgctcagga acggtaagga
     1021 catttcctgg gatacactca tgaatgctga tcctgtggca tgtgttccgc tcctcgcaac
     1081 cgatccactc tatctcctgt acacatcggg aaccacggga aaacccaaag gtgtagtaag
     1141 ggataacgga gggcacctcg tagccctgaa atggagtatg aaaaatatct atggagtccg
     1201 tcccggtgaa gtctactggg cggcttccga tatcggctgg gttgttggac attcgtatat
     1261 tgtatacgga ccattattct atggctgcac tactatcctc tacgaaggaa aatcggtcgg
     1321 cacaccggat gccggtgcat tctggcgtgt catttcggag cacggtgtgt cagtcctttt
     1381 ttgcgctccc acggcatttc gcgccatccg caaggaagat ccggagggta ggctgataca
     1441 taattacgat ctcacacggt tccggatgct cttcttagcg ggtgaacggt gtgacccaga
     1501 taccctggtc tgggcacaac aacagctgaa ggtaccggtt atcgatcact ggtggcagac
     1561 cgagaccggc tgggcgattg gtgcaaactg tgtcggcctt gaactgctcc ccgtaaagcc
     1621 aggttcatgt accaggccgg tgccaggatt cgatatctgt gtgcttgatg caaagggaga
     1681 aaaagtacct gcaggtgaaa cgggaaatat tgttgtcaga caaccacttc ccccgggatg
     1741 tttcatgaca ctctggcaga atgacaagga ttttgtcaaa acgtattttt ccgcgtatcc
     1801 gggttattac ctgacttcag atgccgggta catcgatacc gaagggtatc tctggataat
     1861 gggcaggacc gatgacatca ttaatatcgc cggacaccgg ctttctaccg gtgcaatgga
     1921 agaggtactt gcatctcacc cggatgttgc ggaatgtgca gttgcgggag tacatgatcc
     1981 ggtaaaaggt gaagttcctc tgggttttgt cgtcattaag gccggagtta caaaactccc
     2041 cgacgcaatc agttctgaac ttatttccct tgtacggcag aaaatcggac cgattgcgtc
     2101 ttttaaagct gctgctgtgg taaaaagatt acccaaaacc cgatcaggca agattctgcg
     2161 ggggactatt aaaaaaatcg ctgatggtgt tccttatacg gttccggcga caattgatga
     2221 ccctgtgatc cttgatgaga ttactttaac tttgaggaaa atggggtatc caaaaacata
     2281 aaatggaaaa aagagggcag cccgttattc ggtatttcac gttctgtata aagtaaaaaa
     2341 gtaccctaaa gatacaatag ccgatttttt tctgtattcc cgatcccgct ttgacttttc
     2401 cttaggtgct gcaatctttg ctgggggaga tccgcagatc aggtaccctc gacctggatc
     2461 gcttcaatac atatcctgac aatgagtcca tcatcagtga actggtaaag atccggggtg
     2521 tgggcaaggc ggactgcgga gctcaccctc atgagaggat tacctcgtca tgacgcgttc
     2581 cccgctgatg atgttggcgt gcgacggttc atatcacaat tttaccggaa ggtgagaaga
     2641 cctcatatat ggaggcaagg gcaattgcat acgttgggga gcctggaaag gatttgccgc
     2701 atattatctc gaaatcgcag acctgctcag gatcgacccc aataatctcc aaaatatatg
     2761 aaaaacatta ctccacgaac gagcctaacg ccgcaggttc accatgctca tggttcagaa
     2821 agaaacgtga tatacctgtg tacatttttc tgaagtgaac aatgcattta ccgcttgaga
     2881 aggaaaaacg gtaaaattat ggaataccga gcc
//
LOCUS       DASRLV010000105         4872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_358292,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000105 DASRLV010000000
VERSION     DASRLV010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358292"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1952)
                     /locus_tag="VFC43_04255"
     CDS             complement(<1..1952)
                     /locus_tag="VFC43_04255"
                     /inference="COORDINATES: protein motif:HMM:NF044377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin/FtsZ family protein"
                     /protein_id="HZK30429.1"
                     /translation="MRILAIGLGGAGCRIVNSLYTTDRRSSKVVCVQALAIDVHADTL
                     AQLGGLPDNAKLYFPPVDPDIQTEDNGDAQTATIDIGEIVARIQNVEIGETDAIFVCC
                     GLGGSMVDVAPHIIAALRSSIVEPIFGLVTLPCLAEGERHSSKAADDLDMLTPLLDGI
                     ILFDNETWYKKIKALKSTLIKKERGLAEKLGFRKSEPEISSELATYLLLNEGIVRRIS
                     LILRAGEFKADGGLDFAEVVLDSGEVLNTMKGMGYITIGYAVERLPHNPLGFLTSLRP
                     AVFFADEHNKKASRIVDLAKQAIYHEISTPCDMTSAHKALILIAGPSQELSLKGFMTV
                     RKWIDRSIAGLETRSGDYPVMNTKNVAIIIMLSGLENIPRITELKEIQNQYKNKHQDS
                     AGIGIRNRTPTIQGDDSKKDDMLILTGTAYKKETSSWRDSVRISHQENDIIPEIGAPD
                     EVISSRPALRRKISSESEAAGKKTIPHHITKHQIVEESIPQLHKNDAGMRKTTGADVS
                     QNRVVKAKDSDNQTVQSAPDITSHISPEHGTRPGKKSSYTKDSRGFDEKHLPRTIEGR
                     LKPKEIERQIIEKELQRQRMMAISGRTQKTGSEVSKHPSHPHEIIRLKREVHEKTSPE
                     RDDAQSDLAEEMQPLPKKRRIIIQKKT"
     gene            2403..3389
                     /locus_tag="VFC43_04260"
     CDS             2403..3389
                     /locus_tag="VFC43_04260"
                     /EC_number="1.5.98.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5,10-methylenetetrahydromethanopterin reductase"
                     /protein_id="HZK30430.1"
                     /translation="MTYGIEFVPGNVNVKQVVNFCKLAESKDIDFAWITNHYNNRHCY
                     PTLAAIAQATTSLKMGPGIMNAFTDTPAAMASFAATLNEISDGRAVLGIGPGDLSTLP
                     KLAINAEKPVGRLEEAVVQIRKLLAGEEIKKSGMQFFDYDGAKLTGVTLPGKKGIPIY
                     IGAQGPKVLDLAGRIGDGALINASNPKDFAIAIPIIQAAADKVGKKGFDIGAYTAMSI
                     DRSEKKARNSAKIVAAFIAAGSPPDLLTRHGLDLANVAKIKAALGKFDFKTVGELVGD
                     KEIDAFTIAGTPEMVKQKCEALTKAGVTQIIFGSPLGPDMTTSIRLLGKYVV"
     gene            3871..4800
                     /locus_tag="VFC43_04265"
     CDS             3871..4800
                     /locus_tag="VFC43_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HZK30431.1"
                     /translation="MFSVKNVVTSRGTLQYREEHLTGIRCRISPDRLKRHIDQTFYIP
                     SKAEGCPFCRDAVFFVTPTFPDGRRIIHGESVTFPNLFPFGEGHVVTVITREHAVAAF
                     SLLQIVDALIAQIDALQQTDGYASINWNFLPSAGASLVHPHMQGLSDSCPSRIVDSYI
                     TASEQFRRKQGRNYWDAVWDQEKSSDRYLFGDEMLWSAHAVPVGEREVRGLLPISTID
                     EMENYADLVARGILEIISLYRELGTHAFNMSLFFDKKGNDHGFRAFCSMISRINPNPL
                     STSDSAFMERLHMEPVIMTLPEDLGKFYKKEKN"
BASE COUNT         1273 a         1168 c         1096 g         1335 t
ORIGIN      
        1 gtcttttttt ggatgataat tcttcttttc ttcggcaatg gttgcatttc ttcagccaga
       61 tcagattggg catcatctcg ctcgggggac gtcttttcat gaacttcccg ttttagcctg
      121 atgatttcat gaggatgcga cgggtgcttt gacacttcag atccggtttt ttgggtgcgg
      181 ccagaaattg ccatcattct ctggcgttgc agttctttct cgattatctg ccgttcgatt
      241 tccttgggtt tcaaccgtcc ttcaattgta cgaggcaggt gtttttcatc aaaccctcgg
      301 gaatctttgg tgtacgagga ttttttgccg ggacgtgttc catgttctgg tgatatatga
      361 gaggttatat caggtgctga ttgcacggtt tgattatcag aatccttcgc tttaactacc
      421 cggttctggg atacatcggc accagtagtc ttacgcatac ctgcatcatt tttatgcagc
      481 tgaggaatcg actcttctac gatctgatgt ttcgttatat ggtgaggaat ggtctttttt
      541 ccggcagctt ctgattccga agatattttt cgccgtaacg caggccggga ggagatcact
      601 tcatcgggag ccccaatctc aggaatgatg tcattctcct ggtgcgaaat acgtacggaa
      661 tcgcgccacg aggaagtttc ttttttatac gccgttcccg taagaataag catatcatct
      721 tttttgctgt catctccttg tattgtcggt gttctgtttc ggataccgat cccagcacta
      781 tcctgatgtt tgtttttata ctggttctgg atctccttaa gttcggtgat acgggggata
      841 ttttcaagcc cggacagcat aatgataatt gcaacatttt ttgtattcat tacaggataa
      901 tcaccggatc gcgtttccag cccggcaata cttctgtcga tccatttacg taccgtcata
      961 aaccctttca gggaaagctc ctgagatgga ccggcgataa gaattaatgc cttgtgcgcg
     1021 ctcgtcatat cgcagggtgt tgatatctca tgatagatcg cctgctttgc gagatcaaca
     1081 attcttgagg cttttttatt atgctcatcc gcaaaaaaaa cggctgggcg caaactggtt
     1141 aagaaaccca atggattatg ggggagacgt tcaactgcat acccgatagt aatatacccc
     1201 atccctttca tggtattgag aacctcgccg gagtccagta caacctctgc aaaatcaagc
     1261 ccgccatctg ctttgaattc tccggctctc agaatgaggc tgattctcct gacgataccc
     1321 tcgttcagga gtaaatatgt ggcaagttcc gaagaaatct cgggttcact tttcctgaac
     1381 ccgagttttt ctgccaatcc cctctctttt ttgatcagtg tggatttcag tgccttgatt
     1441 tttttatacc aggtttcgtt atcaaacaga ataataccat caaggagtgg tgtcagcata
     1501 tcgagatcat ctgctgcttt tgatgaatga cgctcccctt cagcaagaca gggaagtgta
     1561 acaagaccaa agattggctc tacaatcgag gaacgaagtg ctgcgataat gtgcggtgca
     1621 acatccacca tgctgccgcc aagcccgcaa cacacaaaaa ttgcatcagt ctctccaatc
     1681 tcaacgttct ggattcgggc aacaatttcc ccgatatcga tcgttgcggt ctgggcatct
     1741 ccattgtcct ctgtctgaat gtccgggtcg acaggaggga aataaagttt tgcgttatcc
     1801 ggcagacccc ccagctgggc taatgtatca gcatgtacat cgattgccag tgcctgaacg
     1861 caaacgactt tactgctacg cctgtctgtt gtataaagac tattaacaat cctgcagcca
     1921 gcgccgccca gtccgatagc aagtatacgc atgaacaaat cctaattaaa tccttattgg
     1981 caccctatag ctcaatgtac tattatttgc tggaagttga atatcttata gtctactgct
     2041 tcgttgcggg aaatagtaag tatattggaa ttttttttaa aaaaatacaa ctccaaaaac
     2101 ggttatagat aaaaaaaata atattatttt cggttatgaa tcaagcagga tgaattcatt
     2161 gcccgttgca tttttttttg gaatcggaaa ttttaattgt tctataggga agaaacgttg
     2221 gaggaacgac tgaatgagca tatctcggtg cgttcacccc cgctcaatga ccctccttgc
     2281 atatctagta tgaaaaaaac gataaaatgg gcatattttg agcggatagc gcaaaagggt
     2341 caacgtaaaa tgataattta tataaatgcg tggacggtaa tattgcatga ggtgaataac
     2401 ccttgacata tggaattgaa tttgtaccag gaaatgtcaa tgtaaagcaa gttgtaaact
     2461 tctgtaaact tgcagaatcc aaagacatag atttcgcatg gatcactaac cactacaaca
     2521 accgtcactg ctaccccacg ctcgccgcga ttgctcaggc aactacgtcc ctgaaaatgg
     2581 gcccgggtat tatgaacgca tttaccgata cccccgctgc aatggcgtca ttcgcagcca
     2641 cactcaacga gatttccgat ggacgtgcgg ttctcggtat tggacccggg gacctctcca
     2701 cgctcccgaa gcttgcaatt aatgctgaga agccggtcgg acgcctcgag gaagccgttg
     2761 tccagatccg aaagctcctt gctggtgagg aaatcaaaaa gtcaggaatg cagttctttg
     2821 attacgatgg cgcaaagctc accggagtta ccctgcccgg aaagaagggc atcccgattt
     2881 acatcggtgc ccagggcccc aaggtcttgg atcttgccgg aaggatcggc gacggtgcat
     2941 tgatcaatgc atccaacccc aaggactttg ccattgcaat accgatcatc caggcagccg
     3001 ccgacaaagt cggcaagaaa ggctttgata tcggtgcata caccgcaatg tcgatcgacc
     3061 ggagcgagaa gaaggcacgt aactcagcaa agatcgttgc cgcattcatt gcagccggct
     3121 cacccccaga cctcctcacc cgccacggac tcgacctcgc caatgttgca aagatcaagg
     3181 cagcacttgg aaagtttgac ttcaagacgg tcggagagct cgttggggac aaggagatcg
     3241 acgccttcac cattgcagga acccctgaga tggtcaagca gaaatgcgaa gcactcacca
     3301 aggccggtgt cacccagatc atcttcggct caccccttgg tcccgacatg accacatcga
     3361 tccgcctgct cggtaagtac gtagtataac cgcagcacac aatacctgcc gggatacaat
     3421 ccaaaaggca ttgatcccgg cagatattac ccgtttcttt ttttcgctca cttatccgct
     3481 atctcttact tgattgaaaa gatgtcgagc aataaaccgg gattcaatgg tcccttcatc
     3541 agaaacataa ccaatccatt gaaggataac ctgtcggatg aaatcctcat ttttgaccat
     3601 tagtacccca atagcgcatg aggtgagcgg atccatttag ggtgaaggac accccaatct
     3661 cctcatttga tgtgatccct ctggtggcga aatcatcacc aaaaacaaag aatgtttccc
     3721 taactccccg tatttaacaa ccccccttca aaaaaatgaa tgatggcgat tcaggggatt
     3781 taccgaaaac tctctggtaa ctctaaccgt tagcagatca ccgtgcaaac gactatctga
     3841 tctcaccgga aatagtatct ttgaacaggt atgttttcgg tcaagaatgt tgtcaccagc
     3901 aggggaacat tacagtatcg cgaagaacac ctcacgggta tacggtgcag aatcagtcct
     3961 gaccggttaa aaaggcacat cgatcagaca ttttacattc catcaaaagc ggagggttgc
     4021 ccgttttgcc gtgacgctgt tttttttgtc actcccacat ttcctgatgg caggcggatc
     4081 atccatggcg agagtgttac tttcccgaac ctgtttcctt tcggggaagg tcatgtagtg
     4141 actgtaataa cccgcgaaca cgcggtcgca gccttcagcc ttctacagat agtggatgct
     4201 ctcatcgctc agatcgatgc acttcagcaa actgatggct atgccagcat aaactggaac
     4261 tttttgccat cagccggtgc gagccttgtg cacccccata tgcagggatt atctgactcg
     4321 tgcccctcac gtatcgttga ctcttatatt accgcaagcg aacagttccg caggaaacaa
     4381 ggaaggaact actgggatgc agtatgggat caggaaaaat cttctgatcg ttatctgttc
     4441 ggtgatgaaa tgctctggtc tgcccatgcg gtaccggttg gtgagcgcga agtgagggga
     4501 ttactcccga tatccacgat tgatgaaatg gaaaattatg cagatctcgt agcacggggt
     4561 atacttgaga ttatctcgtt ataccgggaa ctggggaccc atgcgttcaa tatgtccctt
     4621 ttttttgata aaaaaggtaa tgatcatgga tttcgtgcgt tttgctcaat gatctcccga
     4681 attaacccga accctttatc gacaagtgat tccgcattta tggaacggct tcatatggaa
     4741 ccggtaataa tgacacttcc cgaagacttg ggaaaattct acaaaaaaga gaaaaattaa
     4801 aaaaatttac tcgtattttg caacgagttt cttcttggag acaagagtgc catctttctt
     4861 gtgggcctgg cg
//
LOCUS       DASRLV010000106         7606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_199178,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000106 DASRLV010000000
VERSION     DASRLV010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 7606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7606
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199178"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VFC43_04270"
     CDS             complement(<1..571)
                     /locus_tag="VFC43_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HZK30432.1"
                     /translation="MSPKKDNSPSLDDQRKKILVSKNGPYIVSGRIPLIKKEIRNDAD
                     GYCSTWHDVETYPLQEQYALCRCGHSKNKPFCDGTHTKIHFDGTETADDIPYLERAEK
                     ITGPALSLTDADNMCVHARFCMRAGGIWNLTRQSGNPETRDLAIEEAGNCPSGRLVVW
                     DRKAGEAIEPEFEKSIVIIEYPPKSEHGPL"
     gene            complement(682..1302)
                     /locus_tag="VFC43_04275"
     CDS             complement(682..1302)
                     /locus_tag="VFC43_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2115 domain-containing protein"
                     /protein_id="HZK30433.1"
                     /translation="MENGQFLQPADKPARARGDIQEIADRLSTARTKGELGLILIHEM
                     RVYTIFDLQIIGGRLNNEIDKLPSPYRERIRPYFREQLFGKHHQLLAMHHNKAFAGMT
                     SPIRDPATFKKFCTMVTEGVFSWQDSSERNPYFRNPKNRLFYYLISAYTMFILDEPGH
                     PEGMPFPGGFSVERRGSEYYCLIRDKEKEIVYSICNFCPAHQTEES"
     gene            complement(1423..2358)
                     /locus_tag="VFC43_04280"
     CDS             complement(1423..2358)
                     /locus_tag="VFC43_04280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HZK30434.1"
                     /translation="MSIVQCCSSPGCDFIQRFRFKKIDAFVEGQSTGNPAGCVYLKDQ
                     KDLSVEGMQQIARELAGFVSEVVYVYPDEGHIGLRYFSSEREVSFCGHATIAVMYDLI
                     KNDEAVARDPIVRIHVQGRDLLVRNEIASADAVFISAPLPRFLDTDLTRDDIAAALKI
                     PPGEIAEKNRIMLVDAGLATLILPLTSLDVLLALRPDQKHLRDFCLAKGIEIILVFSE
                     EVSIKGNNYHTRVFAPIYGYLEDPATGSGNAALGYYLLNAGLWDGQMLKIDQGRNRER
                     PNIIRLVSDASKVQRSVFFGGNAMVRIEGEYLLYQ"
     gene            complement(2945..4036)
                     /gene="dinB"
                     /locus_tag="VFC43_04285"
     CDS             complement(2945..4036)
                     /gene="dinB"
                     /locus_tag="VFC43_04285"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HZK30435.1"
                     /translation="MQPGITQGTTPRIILHLDMDSFYASVEVREQPELRGKPVVIGAD
                     PKNGKGRGVVSTCSYEARAFGIRSAMPVSQAFVLCPHAVFLPPYFSRYVKASSDIMSI
                     LKTLDFPLQQVSIDEAFLDVSRLGTFAAATDLAVKIKDTILTQLGLTCSIGLGPTKVV
                     AKIASDIKKPDGLTVVTPENLFSFLAPMPVRKIPGIGKKAEAVLFEMGIMTIQDLAAF
                     DIQVLIARFGRSAITLKEITAGIDTSEVKERDCVQSVSRETTFANDTSDRQLISATIN
                     ALAEEVCRNLSDETLHCRTVTVKVRYQGFITKTKARTLPHYTGDPETIRSCAFSLLRD
                     IFDGRKIRLLGIRLSSFNTLDGRQRTLGI"
     gene            complement(4071..5255)
                     /locus_tag="VFC43_04290"
     CDS             complement(4071..5255)
                     /locus_tag="VFC43_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nre family DNA repair protein"
                     /protein_id="HZK30436.1"
                     /translation="MIRKGAYLKQLTAATLQMKSVTVGKDLEGSSPPSVFIGSWNYPD
                     VYAGPMIAAVHGDTAFMDMPESWISGHKTQEEIIGYRLNLVRGKHRVNAADLDNRFIE
                     KLQEISLSDTSVESDVSFKNIPAGTSFSEEHTPFGPSASIERFEIESGRWDRNLEKVY
                     YDTDLKSADAVVGLHKNGVPFSSIQKAFSVGITGRDQGRHLVPTRWSITACDTMIGDR
                     LLQEVKRCPVIDTWRVHEYSSLNNNYAVILMPTGWQYEWSEAFLHVLGNEELVFSDHE
                     GIKKKTEYSPLGGCYYSCKMAVLEALAQQQKQAGAIILREALHGYVPMGVFNVRENVR
                     SAMREQGSEFEDIRSVLSHISQKFTLPMQRFIEEGNLLKSILRERQCTLRDFSAMTHV
                     GT"
     gene            complement(5410..7251)
                     /locus_tag="VFC43_04295"
     CDS             complement(5410..7251)
                     /locus_tag="VFC43_04295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZK30437.1"
                     /translation="MNLPQILSKRDIVWDVLIGVTIFLTLSVNLIGLLNGITVALPHL
                     LYIPVVIAAYRYPKWGMFIAGCIGGMYFLMVFLTAGSSISTFLEAFARTLVIIGIGWL
                     IALLSFRLREREDLYQGLFYHSEAGSILVRETDKGRVIEEVNDKASDLLHRTAPALIG
                     APLTSFWSEDFEQKLFSQLLREGAVHAAETIFTLPDGRSETVLVSAALLPDKRVIITF
                     AEITRRVYAEKALKAANDKLKFLSRISHDHLHRTVDQMVLTVKNADTHCNDTLAKGFF
                     TQMRVLAVNLKRQLILTESYKNLGISPPAWFSVQQILKSAYPAFAIGSISIRFWTERL
                     EIYADPLFQDVLTHITGNAIGHGIKIQNLVITYHETSEGLDVILEDDGIGIAADKKHQ
                     IFEYDEMGGEGIGLFICREILGVTGMAISENGTEGKGARFVIHVSPEAYRVEGTSEDA
                     PPRTFPESEATGLRGSRHTTGIIVKELCAVEFPVAETLWTEYHETKGDMKTDRIFVGY
                     AQNEPLSVARCRKHPDGFEVDAVFTPPAQRGHGYAHAVMWGLVEACGNDTLYMHSVTH
                     LTDFYRRFGFVTIAESELPSTIRERYAWAQGEMEGANVSPMKRSPRF"
BASE COUNT         2005 a         1844 c         1740 g         2017 t
ORIGIN      
        1 agagtggacc gtgttcactt tttgggggat attctataat cacaatggat ttttcaaatt
       61 cgggctcaat tgcctctccg gctttcctgt cccagacaac cagacgcccg gaaggacagt
      121 tgcctgcctc ctctatggca aggtccctgg tctcaggatt tcctgattgt cgcgtcaggt
      181 tccaaattcc cccggcccgc atgcaaaatc ttgcgtggac gcacatatta tcggcatcag
      241 tcagcgacag ggcaggcccg gtaatttttt ctgcccgctc gagataaggt atatcatcag
      301 ccgtctctgt tccatcaaag tggatttttg tatgggtgcc atcacagaag ggtttgtttt
      361 tcgaatgtcc gcaccggcac agggcgtatt gttcctgcag gggatatgtc tctacatcat
      421 gccacgtact gcagtaccca tcggcatcgt ttcggatctc ttttttaatg aggggaatac
      481 gtcctgaaac aatataggga ccatttttac tcaccaggat tttcttcctt tggtcatcca
      541 gggaggggct gttatctttt tttggggaca tggtactcct tgaatccttt ttactcattg
      601 ggtcttaaac tgtttcaaat ctataaaatc caattttaac caacatccta aaaaggagta
      661 taagaatcga aaaaaagcaa atcagctctc ttctgtctgg tgagccgggc agaaattgca
      721 aatggaatag acgatctctt tttccttatc ccgtataagg caataatatt cactgccccg
      781 tctttcaaca gaaaaaccgc cgggaaatgg cataccctca ggatgaccgg gctcatcgag
      841 aataaacatg gtgtatgcgg aaataaggta atagaagagg cggttcttcg gattccggaa
      901 atacgggttg cgttcgcttg aatcctgcca ggaaaaaaca ccttccgtaa ccattgtgca
      961 gaatttttta aacgtcgcgg gatcccggat tggactggtc ataccagcaa atgccttgtt
     1021 gtgatgcatt gcaaggagct ggtggtgttt tccgaacagt tgttcacgga aataggggcg
     1081 gatcctctca cggtatggag agggtaattt atcgatctcg ttattcagcc tgcctccgat
     1141 gatctggaga tcgaatattg tgtatacacg catttcatgg atgagaatta aaccgagttc
     1201 gccttttgtc cgcgcggtac tcagtctgtc tgcgatttcc tgtatatcgc cacgtgcccg
     1261 agcaggttta tcagcaggtt gaagaaattg tccgttttcc atcgacaccg aaccttatcc
     1321 agtattattg tatcactgcc gtgaactaaa aaagggatag atgttcttcc gattacttca
     1381 acataatagt acgataccgg aaaaatacga tatttgtgta tattattggt agagcagata
     1441 ttccccttca attctcacca tcgcatttcc accaaaaaag acactgcgct gcacttttga
     1501 tgcgtcgctg accagccgga tgatattggg acgctccctg tttctgccct gatcaatttt
     1561 taacatctgg ccgtcccata gtcccgcgtt gagcaggtag tacccgagtg cagcatttcc
     1621 tgagccagta gccgggtctt ccagataccc gtaaatgggg gcaaataccc gggtatggta
     1681 attattccct ttgatcgaaa cttcttcaga aaagaccagg ataatttcga ttccctttgc
     1741 aagacagaaa tcccgcaggt gcttttgatc agggcgaagc gccaggagga catcaagcga
     1801 tgtaagggga agaatcaggg ttgccagacc ggcatcgacc agcataatcc ggttcttttc
     1861 cgctatttca ccgggcggaa ttttgagggc tgcagcaata tcatcgcggg tcaggtcggt
     1921 atcaagaaat ctgggaagtg gggcactgat aaacacagca tctgctgaag caatctcatt
     1981 ccggacgaga aggtcacgtc cctgtacatg aatacggaca atggggtccc gagcaactgc
     2041 ttcatcgttt tttataaggt cgtacatcac cgcaattgtg gcgtgaccgc aaaaagatac
     2101 ttcgcgctca gaggagaaat accgcagacc aatgtgccct tcatcgggat aaacatagac
     2161 gacctcgctg acaaaacccg caagttcacg ggctatctgc tgcatcccct caacagacag
     2221 atctttctga tccttcaggt acacacatcc tgcgggattc ccggtcgatt gtccctcgac
     2281 aaacgcatcg atttttttaa aacggaagcg ctgaataaaa tcacatccag gactggaaca
     2341 gcattgtact atggacaata tactgttttc agtccgatat ccacagctaa attaatatga
     2401 gctgtatcta tgagggagaa aaaatttata acttccggat cggtgtttat aaatcgtttt
     2461 attcaggttt ataatcctat tccggggggt cttacggcac tgtggaagaa cggcgcaaaa
     2521 aaatgtgaga aatatttaag actcggatcg gtattctccg aaaattttgt ttaactggct
     2581 gaaaatttac atgaaaataa attaaagtta gaattgtgtc atatttaaaa aaataatatt
     2641 taaatactat aagaaataat taaatttaac tgcaacaaac aaaaggaggt tggatgttac
     2701 cggatcgggt ccattgtata atcacagaat tcaccgcaaa tatacagctc tgaaccagct
     2761 ttcacataaa cccagctggt agtttcctcc tatcagattt caataattaa tactgcactt
     2821 tttataattc tccagtacct ccattgctgg aaagtaaggg atatccggaa attttggatg
     2881 tctcttgctt agttctcgag ggtttataac catattcttc cgatggtgct aattattgta
     2941 ataatcaaat ccccagggtc ctctgccggc catccagggt attgaatgat gagaggcgaa
     3001 tgccaagcag tcggatcttc ctgccatcaa aaatgtcacg gagaagggaa aacgcacatg
     3061 agcgtatcgt ttccggatca ccagtataat gcgggagtgt ccgggctttc gtctttgtaa
     3121 taaaaccctg gtacctaact tttacggtaa ccgtcctgca gtgcagggtt tcatcagaga
     3181 gattccgaca gacttcctct gcgagtgcat ttatcgttgc gctaatcagt tgcctatcac
     3241 ttgtgtcgtt tgcaaacgtg gtttcacggg atacagattg tacacagtcc cgttctttta
     3301 cttcacttgt atcaataccc gcagtaattt ccttcaaagt gatcgcggaa cgcccgaacc
     3361 gtgcaatcag aacctggata tcgaatgctg caagatcctg aatagtcatt atgcccattt
     3421 cgaaaagaac cgcctcagct ttctttccaa ttcccggaat tttccggacc ggcatcggag
     3481 caaggaatga gaacaggttc tctggcgtaa caacagtaag tccatcaggt ttcttgatat
     3541 cggaagcaat ctttgcaacc acctttgtcg gcccgagccc gatcgaacag gtgagtccga
     3601 gttgtgtaag aatagtatcc ttaatcttta ctgcgagatc cgttgcggca gcaaatgtcc
     3661 ccagtcggct cacatccagg aacgcttcgt ctatacttac ctgctggaga gggaaatcaa
     3721 gtgttttcag aatcgacata atgtcagaag aagctttcac ataccgggaa aagtacgggg
     3781 gaagaaatac tgcatgcggg cagagaacga atgcctgcga aacgggcata gctgatcgta
     3841 ttccaaatgc acgggcctcg tacgagcagg ttgaaacaac cccacgacct ttgccgttct
     3901 taggatcggc accgattacc acaggctttc ccctgagttc cggctgttcc cgtacttcaa
     3961 ccgaagcata gaaactgtcc atatccaggt ggaggatgat cctgggtgtc gtcccctgag
     4021 tgattcctgg ctgcattacg aaaacctcac cattgctcac attcaggtat tcatgttcca
     4081 acgtgtgtca ttgcagaaaa atcgcgcagc gtgcactgcc gttcccgtag tatgctcttc
     4141 aacaggtttc cttcttcaat aaaccgctgc atgggcaggg taaatttttg cgagatatgg
     4201 gaaagaactg agcgaatatc ttcaaactca cttccctgct cccgcatcgc gctcctcaca
     4261 ttctcccgta cattgaatac acccatagga acatacccgt ggagtgcttc ccgcaggata
     4321 atggcgccag cctgtttttg ttgttgtgca agtgcctcca gcaccgccat cttacaggaa
     4381 tagtaacatc cacccagtgg tgaatattct gttttctttt taatcccctc gtgatcagag
     4441 aacaccagtt cctcattacc cagcacgtga aggaatgctt ccgaccattc gtactgccag
     4501 ccggttggca taagtatcac tgcgtaatta ttgttgaggc ttgaatattc atgcacccgc
     4561 cacgtatcga tgaccgggca ccgtttcact tcctggagaa gccggtcacc gatcatggtg
     4621 tcgcaggctg tgatcgacca acgggttggg acaaggtgcc gcccctggtc cctacccgtt
     4681 atgccgactg aaaatgcctt ctgtatgctg gaaaatggta caccgttttt gtgcagacca
     4741 acaaccgcat ctgcagattt caggtcggta tcatagtaaa ctttctcaag gtttctgtcc
     4801 caccggccac tttcaatctc aaacctttcg atcgaagcgc tgggcccaaa gggggtgtgt
     4861 tcttctgaaa atgaagttcc ggcagggata tttttaaacg aaacatcgct ctcaacagac
     4921 gtgtccgaaa gggagatctc ctggagtttt tcgatgaacc ggttatcaag atcagctgca
     4981 ttaaccctgt gtttaccccg taccaggttg agccggtaac caataatctc ctcctgtgtc
     5041 ttgtgccctg atatccacga ttcaggcata tccatgaatg ccgtgtcgcc atggacagca
     5101 gcaatcatag gaccggcata cacgtcaggg taattccagc tccctatgaa cacagatgga
     5161 ggcgagctgc cctccagatc ttttccgact gtcaccgatt tcatctgcag ggtagcggct
     5221 gtcagctgtt tgagatatgc accctttcta atcatcacta tgagagagga ttaacaggtt
     5281 ctctataaag gagtgaggtg tgcagggaga aagtgaaccg gtaacgtgta gcagactgga
     5341 gtaagatatg gataatcgta actcatgtat gaccggacaa ggtaatgaac cggtgggaca
     5401 ccatttttgt cagaaacggg gagatcgttt cataggagag acattggcac cctccatctc
     5461 tccttgtgcc catgcatacc tctcccggat tgttgaagga agttcactct ctgctatcgt
     5521 gacaaaacca aaacgacggt aaaagtctgt gaggtgtgtc actgagtgca tatagagggt
     5581 atcattcccg caagcctcaa ccaggcccca cattacggca tgtgcatacc catgaccccg
     5641 ttgagcgggt ggagtgaaaa cagcatctac ctcgaatccg tcaggatgct tccggcaccg
     5701 cgcaactgat aggggctcat tctgtgcata tcccacaaat atccggtcag tcttcatatc
     5761 tccctttgtc tcgtgatatt ctgtccatag ggtttctgca acaggaaact ccactgcaca
     5821 aagttcctta acaattatac ctgtcgtatg ccgggaacca cgaagacctg ttgcctcact
     5881 ttccggaaaa gtgcgcggag gtgcatcttc actggttcct tccacccggt aagcctcggg
     5941 agagacatgg atcacgaaac gtgccccttt tccttccgtc ccattctctg aaatagccat
     6001 accggttact ccgaggatct cccgacagat gaataacccg atcccttcac cccccatttc
     6061 gtcatactca aaaatctgat gctttttatc tgccgcgatc cctatgccgt catcttccag
     6121 aatgacatcg agtccttcac tggtctcatg ataggtgatg acgaggtttt gaattttgat
     6181 cccgtgaccg atggcgttcc ctgtgatatg ggtaagaacg tcctgaaaga gaggatcggc
     6241 ataaatttca agtctctccg tccagaaacg gatagaaatg gatccgatgg caaaggcagg
     6301 atatgcagat ttgagaatct gctggacact gaaccatgct ggtggggaga tcccgagatt
     6361 tttatatgac tcggtaagaa tcagctgccg ttttaaattt acagcaagca cccgcatctg
     6421 ggtaaaaaaa ccctttgcca gagtatcgtt gcagtgtgta tccgcatttt ttaccgtcag
     6481 gaccatctgg tcaacagtac gatgaaggtg atcgtgggaa atgcgagaaa ggaatttaag
     6541 tttgtcatta gcagccttca gtgctttctc agcataaaca cgtcgggtga tttctgcaaa
     6601 tgtgataatt acccgcttgt ccggaagtaa tgctgccgag acaagaaccg tttcagacct
     6661 tccatccggt aaggtaaata tggtctctgc cgcatggacc gcgccttccc tcaggagctg
     6721 tgaaaacagc ttctgttcaa agtcctcact ccagaatgag gtaagtggtg ccccaataag
     6781 agcaggtgct gtcctgtgaa ggaggtcgga ggctttgtca ttcacttcct caattactct
     6841 ccccttatcc gtttcccgga caagaatact tcctgcctct gaatggtaaa agagcccctg
     6901 atacaggtcc tcgcgttcac ggagcctgaa ggaaagcaag gcaataagcc acccaatccc
     6961 gataatgacg agtgtcctgg caaatgcctc gaggaaggta ctgattgagc ttcctgcagt
     7021 caggaacacc ataagaaaat acatcccgcc gatgcatcct gcgatgaaca tgccccactt
     7081 tggataacga tatgctgcaa ttaccaccgg gatatagaga aggtggggaa gtgctaccgt
     7141 tataccattt aaaaggccga tgagatttac agacagggtc aaaaatattg tgacacctat
     7201 cagcacatcc cagacaatat cgcgttttga cagaatttga gggaggttca cgggaagatt
     7261 ctctccttta tcatacgagg gactggctgg tgaccgccgg acaacttctc atcaatgctg
     7321 ttcaatgccg gccattgata cgcctcacct gatgcagact accaaggaag attgttgtcg
     7381 gtcgaattta ttatcattcc ttttcttttt tgcaggatcc ggcgtatgtg acacccgcaa
     7441 tacatatgtt aatcgttcat gacatcattc atatacctca ccggagttgt gtgaattaga
     7501 aataataaca gtggaaataa tgacggtatc gacccgaatg cagtaaatga agacgggact
     7561 cctgttgtca gggttcggtt accaaagaaa tgggccaacg aacaat
//
LOCUS       DASRLV010000107         7213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_770270,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000107 DASRLV010000000
VERSION     DASRLV010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 7213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7213
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770270"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..652
                     /locus_tag="VFC43_04300"
     CDS             251..652
                     /locus_tag="VFC43_04300"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HZK30438.1"
                     /translation="MNVVPDQDCVQCGRCCEKWGWGQKGIIEDLIPWIEENRSDILKH
                     VSVTLKDKKKRNGRDISRNDLAGIIRIDYWVSPDGRALTYCPFYTLADDGKVYCAIHR
                     AKPRVCIGFEPWNEGIRDYALNCPACRNTAP"
     gene            complement(906..2099)
                     /locus_tag="VFC43_04305"
     CDS             complement(906..2099)
                     /locus_tag="VFC43_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HZK30439.1"
                     /translation="MKKKTVTREHDDGVPHIGEARKKLLQKLAREEGTPIFVIDHEKI
                     RSNYREFKKHLPNVQVYYAVKANSNPEIVKTLYEMGSSFDVASMPEFMIVYENIKKMP
                     KKERQAWIWDKIIYANTIKPIETLEALDQYNPLVTFDNLEELKKLRQHAPHAGLVLRI
                     RVPNTGSMVELSSKFGAHPGEAVDLITEAFSLGLVVEGLSFHVGSQCTNFENYVQALQ
                     ISAEIIKETETRTGRKIRILDIGGGFPVKYHPEVKSVRTLAKKLETEIKRLFPPDMEI
                     LAEPGRFLVANACMVVAKVVGKAFRDGKPCYYINDGVYHTYSGQVFDHVTYPVLAFQD
                     GDTQLSAVFGPTCDAFDTITLSADLPDLQIGDLVYSENIGAYSHASSTYFNGFPPAKV
                     VHINR"
     gene            complement(2131..3573)
                     /locus_tag="VFC43_04310"
     CDS             complement(2131..3573)
                     /locus_tag="VFC43_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HZK30440.1"
                     /translation="MEFNNKVLIIGYGAVAKCTLPILLRHVTIPLEHITIIDFKDKAQ
                     DLKAWTSGGLRFFKRKVTPGNLSQILSEYLSGGGLLIDLAWNIDCCEMVQWCHDHNVL
                     YVNTSVEAWDPDAEKFTASPYEKTLYYRQMKLHELTKDWHDVATSVVDHGANPGLISH
                     FARQGLCDIARRMIADDIAPDPGLMNDLISRQNFAGLAMETGIKVIHCSERDTQISRS
                     PKKVDEFVGTWCIEGLREEGTAPAEIGWGTHEKELPDQAHVPPVGPKNEILLARMGIN
                     TWVRSWLPHHEIVGMVIRHGEAFGISDRWTVWKDGKAIYRPTVHYAYLPCDATISSLH
                     ELRGRNYELQPKLRILNDREIISGADILGALLMGHPYKSWWTGSILSIEEAKTLAPGQ
                     NATTVQVALGVVTAVMWMIENPKKGFCLPDDLPHGCVLDIAKPYLGEFYSGPSDWTPL
                     KNRKIYFRENPENNYDYDDVWQFKNFLFVR"
     gene            3766..3948
                     /locus_tag="VFC43_04315"
     CDS             3766..3948
                     /locus_tag="VFC43_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30441.1"
                     /translation="MSKRAVDITFQALYTLTDIRAIFRETAPQHMLDDQQRAQAERLL
                     ENLERQVTSLRQEILK"
     gene            3945..5129
                     /gene="pscS"
                     /locus_tag="VFC43_04320"
     CDS             3945..5129
                     /gene="pscS"
                     /locus_tag="VFC43_04320"
                     /EC_number="2.5.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-phospho-L-seryl-tRNA:Cys-tRNA synthase"
                     /protein_id="HZK30442.1"
                     /translation="MRCANGIEARQVNELYINIDPIQAGGKLTPDAMKAVIAYGDGYS
                     VCDNCRKPNRLDYISKPPIAGFHRDLAAWLNMDAVRTVPGARRGFQAVAHTYVGKGDP
                     VLLSSLSHYTEFLAVEETGGIAREIPVGPDHTVTADATAMKIEEVRREFGKVPVLAFI
                     DHVDYQYGNMHDVKAIAKTAHQYDFPVLYNGAYTVGILPVDGKSLGVDFVVGSGHKSM
                     AAPAPSGILAATQERAAEVFRTTAIEGDVTKRKFGIKEPEMMGCTLMGATLVGMMASF
                     PQVKERVKHFDRELENNRIVLESLLSISGTRILSEYPRKHTLTRVDTTGSFDKVAQNH
                     KKRGFYLSGALEERGITGVIPGATKIWKFNTYGTTKKQAQHLAGAFIDIANENGIPRV
                     PQ"
     gene            5396..6871
                     /gene="pap"
                     /locus_tag="VFC43_04325"
     CDS             5396..6871
                     /gene="pap"
                     /locus_tag="VFC43_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HZK30443.1"
                     /translation="MFEKYDLTKTTDDASFEKAITPLKDRLSILQRTLRDLKIPVIII
                     IEGWNASGITMSTKEIIQALDPRGFTLHATDKATDEERARPFMWRFWIKTPAKGRIAL
                     FARGWYSRALAEKLVIIGWKRSLKEEINSITIFERQLCDSGVIIIKFFLHISKDEQKL
                     RLLERERNPLTAWLVTPSIWDFHHNYDTYLPLIDEFIEKTDTAYAPWHIIEATDRKYA
                     ILKVYSHLVKNLERYVADYKGAAGKTKQKSIQKPPKNPVQRKSSSASSYSKEECQELL
                     NNLQIEMLELNYVLFKRKIPFIIVYEGWDAAGKGGNITRLARYINPLGYNVVPVAAPN
                     DYEKEHHYLWRFIRHYPRAGYFAIYDRSWYGRVLVERVEGFCAESEWQRAYQEINEME
                     QDYVESSGGGMVKFWLEISKEEQLKRFHQREKDPLKHYKITEEDWRNRENWDHYDAAV
                     DEMLARTNTSYAPWVVIESDNKWYARIKAVKEVIRYAEKLL"
     gene            complement(7001..>7213)
                     /locus_tag="VFC43_04330"
     CDS             complement(7001..>7213)
                     /locus_tag="VFC43_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30444.1"
                     /translation="GLFKIYKNIAPRTTIAIIVRINIELLGCSSDISFEFFLIFITLS
                     KLRGPESFLIGKEGHTISDSGGCCYN"
BASE COUNT         1943 a         1731 c         1692 g         1847 t
ORIGIN      
        1 acataccggg attccgggtt caactcgccg caacgggctg gaatatacct gccgggttct
       61 gacaggtttt taaattccgc gtacccgatc acgcttgcaa tgctgtcggt atctggttgc
      121 cggtgaccga aaagatatac tgtatgcatc aggggcctct tctggtattg ctaaatctct
      181 ataataaatg accattggag ctcataaggt acagcaacca accaaggtat atccaattcc
      241 tgaccggaga atgaacgtgg tacctgacca ggactgtgtc cagtgcggcc gctgctgtga
      301 aaaatgggga tggggccaga aaggcattat cgaagacctt atcccctgga tagaggaaaa
      361 caggtccgat atcctcaaac acgtttctgt taccttaaaa gataaaaaaa agcgtaatgg
      421 aagggacatt tccagaaacg atctggccgg tatcataaga attgattact gggtcagtcc
      481 tgatggcagg gcattaacgt attgcccgtt ctatacgttg gcggatgacg gaaaagttta
      541 ttgtgctatt caccgggcaa aacccagggt ctgcatcggc tttgaaccct ggaatgaagg
      601 tattcgtgat tatgcactga actgcccggc atgccggaat actgccccgt gatcatgacc
      661 aaaaaattca atcgtaaagt ttttttaaaa ccttaaaaaa attggctctg tagagatcat
      721 tctaaaaatt ctaacgctct ttggggcttt gcccccgcaa ctgcggcatc cccattgcga
      781 tgacgaggta ttacctgata tcacactcgg gttatcgcac tgcgccccgt ccaaacgggg
      841 gactggtggc gcaagagggg gcattatgat aatcgaaaaa tagtcttcac cattgccaaa
      901 aaaaattacc ggtttatatg cacgactttt gcaggaggga aaccgttaaa atacgtggag
      961 gaagcatggg aataggcacc aatattttca gaatacacca ggtccccgat ctggagatcc
     1021 ggcaggtcag ctgaaagcgt gatggtatca aaagcatcac aggtaggccc aaaaactgct
     1081 gacagctggg tatcgccatc ctggaatgcc agtaccgggt aggtaacatg gtcaaagacc
     1141 tggccggaat acgtgtgata cacaccgtca ttgatataat agcagggctt cccgtcgcgg
     1201 aacgcttttc cgacaacctt tgcaaccacc atacaggcat ttgccactag gaaccttccc
     1261 ggttctgcaa ggatctccat atccggcggg aataaccgtt ttatttccgt ctctaatttt
     1321 tttgcaagcg ttcgtacgga ttttacttca ggatggtatt tcaccggaaa accgcccccg
     1381 atatccagga tcctgatctt acggccggtc cgtgtttcag tctcttttat gatctctgct
     1441 gaaatctgga gcgcctggac ataattctca aaatttgtac actggctgcc gacatggaaa
     1501 cttaatccct cgacaacgag ccccagggaa aatgcctcgg ttatcaggtc cactgcttca
     1561 cccgggtgag ccccgaactt tgaagagagt tcaaccatgg aacccgtgtt agggacccgt
     1621 atccgcaaca caagtccggc gtgaggcgcg tgctgcctga gttttttcaa ttcttccagg
     1681 ttatcaaatg tgacaagcgg gttatactgg tcaagggctt ctaaggtttc aatgggcttt
     1741 atcgtatttg catagatgat cttatcccag atccatgcct gtcgttcttt ctttggcatc
     1801 tttttgatat tttcatacac gatcatgaac tcgggcatgg aagccacatc aaaactgctt
     1861 cccatctcgt acagcgtctt cacgatctcg ggatttgaat tggccttgac cgcatagtac
     1921 acctggacgt tggggagatg ttttttgaat tcccggtaat tcgagcggat tttttcatgg
     1981 tcgattacaa aaatgggggt tccttcttcc cgtgccagct tttgcagcag tttcttcctt
     2041 gcctctccaa tatgaggcac cccgtcatca tgttccctgg ttacagtttt ctttttcatt
     2101 acctgatccc acctttgtta ttagagtgat tcatcgcaca aataagaagt ttttgaattg
     2161 ccagacatca tcataatcat aattgttctc aggattctcc cggaagtata ttttccggtt
     2221 tttcaagggt gtccagtctg atggccccga ataaaattca cccaggtacg gctttgctat
     2281 gtcgagcaca catccatggg gaagatcgtc aggaaggcag aagccctttt ttggattttc
     2341 tatcatccac atcacggcag tgacaacccc cagagcaacc tgtaccgtag ttgcattctg
     2401 cccgggtgcc agagtctttg cttcctcaat ggacaggata cttcctgtcc accatgattt
     2461 atacggatga cccatcagga gagcacctaa tatatctgca ccggaaatga tttcccggtc
     2521 attaagtatc cgcagtttgg gttgtaattc ataatttctg ccacggagtt catgaaggga
     2581 tgaaatggtg gcgtcacacg gcaggtacgc gtagtgtacg gtcgggcggt agattgcctt
     2641 gccatccttc cagacagtcc agcggtctga tatgccaaat gcttccccgt gccggatgac
     2701 catgcccaca atttcatgat gcggaagcca agagcggacc caggtattga ttcccatccg
     2761 ggcaagcagg atctcatttt ttggcccgac cgggggtaca tgggcctggt cgggcagctc
     2821 cttttcatgc gtcccccacc cgatctccgc aggagcggtc ccttcctccc tcagcccttc
     2881 aatacaccag gtccctacaa actcatcgac ttttttcggt gaccgggaga tctgtgtatc
     2941 ccgttcagag cagtggataa ctttgatacc ggtctccatg gcaagcccag caaaattctg
     3001 ccggctgatt aaatcgttca taagcccggg gtccggagca atatcatctg caatcatccg
     3061 gcgcgcaata tcacaaagcc cctgacgggc aaaatgggag atcagtccgg gattcgcacc
     3121 gtgatcaaca acagaagtgg caacatcatg ccagtccttt gtcagttcgt gaagtttcat
     3181 ctgccggtag tacagggtct tttcatacgg gctggcggtg aacttttctg catcggggtc
     3241 ccaggcttcg acggatgtat ttacatacag gacattatgg tcatgacacc actggaccat
     3301 ttcacagcaa tcaatattcc atgcaagatc gatcagaagt cctccgccgg agagatattc
     3361 tgaaaggatc tgggaaaggt ttcccggtgt gacctttcgt ttaaaaaacc gcagtcctcc
     3421 cgatgtccaa gccttgagat cctgcgcttt atccttaaaa tcaattatcg tgatatgttc
     3481 aagcggtata gtgacgtgcc ggagcaggat tggaagcgtg catttggcaa ccgcaccata
     3541 cccgataatt aaaaccttgt tattaaattc caatcctgtt cacctgctct tctgaaatgc
     3601 aaaaatactc tggttgtttc tattactata acatagcaaa ctgataagaa aaatttgtcg
     3661 ccgtccaaac aatcattata ggagccgggc ctgcaaaaat atcccggaat ttttttaaaa
     3721 cggttactta tttcatggca ctcgaaatac tagtaaggtg attgattgtc aaaaagggca
     3781 gttgacataa catttcaggc actttacacc ctcacggaca tacgggcaat cttccgcgaa
     3841 accgcgccgc agcatatgct ggatgaccag caacgggctc aggccgagcg gttgcttgag
     3901 aatctcgaac gccaggtaac ctcgctcagg caggagatct taaaatgagg tgcgcaaacg
     3961 ggattgaggc acggcaggta aacgagcttt atatcaatat tgatccgatc caggccggcg
     4021 gaaaactcac gcctgatgca atgaaagccg ttattgctta cggggatgga tattctgtct
     4081 gcgataactg tcgtaaacca aaccgcctcg attatatcag taagccgcca attgccgggt
     4141 tccaccggga ccttgcagca tggctcaaca tggatgctgt gcggacggtt cctggagccc
     4201 ggcgcgggtt ccaggccgtt gcccatacct acgtcggtaa aggcgatccg gtgctccttt
     4261 catcgctttc tcactatacc gagtttctgg cggtcgaaga aaccggtgga attgcccgcg
     4321 agattcccgt tggcccggac cataccgtaa ctgcagatgc aacagcaatg aaaatagaag
     4381 aagtccgccg tgagttcggc aaagtccccg tccttgcatt catcgaccat gttgattacc
     4441 agtatggtaa tatgcatgat gtgaaagcca ttgcaaaaac cgcacaccag tatgatttcc
     4501 cggtgctgta caacggggct tatacggtcg gtatcctgcc ggttgacgga aaaagcctgg
     4561 gtgtggattt tgtggtcggt tccgggcata agagcatggc tgctccggct ccatccggta
     4621 tcctagcagc aacgcaggaa agggcagctg aagttttccg tacaaccgcg attgaagggg
     4681 acgtgacgaa acgaaaattc gggatcaagg aaccggagat gatgggttgt acgcttatgg
     4741 gagcgacact tgtcggaatg atggcgtcat tcccgcaggt aaaagagcgg gtgaagcatt
     4801 ttgaccggga actggaaaat aaccgcatcg tccttgaatc attgctttcc atctcgggca
     4861 caaggatcct gtccgaatac ccgagaaaac atacgcttac ccgtgttgat acaacgggct
     4921 catttgacaa agttgcacag aaccataaga aacggggatt ctatctgtcg ggtgcgcttg
     4981 aagaacgggg aataaccggc gtaattcccg gcgcaacaaa aatctggaaa ttcaacacct
     5041 atggcacgac aaaaaagcag gcacagcatc ttgccggagc gtttatcgac attgcaaatg
     5101 aaaatggcat tccacgtgtc ccacaataat tcatttttac ctttttcttc gttccgcaaa
     5161 tcctaaaaag atacatgccg gcctgacacc tttaggtgaa attcctgtac tttcttttaa
     5221 aaagacgtgt gaaatgtggt ctgaccattg tctatgaaag gattatctga gagactggat
     5281 caatttcccc ggattgaacc atcgcttaac aggtagagtt tattatctgc catgccctac
     5341 gattcgtttg ttaaccgctg caattttttc aattgtcata ccgggagaag ggcggatgtt
     5401 tgaaaaatat gacctgacaa agaccactga tgatgccagc tttgaaaaag ccatcacccc
     5461 cttaaaagac cgtctgagta tccttcagcg gacactcagg gacctcaaaa ttcccgtgat
     5521 catcataatt gaaggctgga acgcatccgg cattaccatg tccaccaaag agattatcca
     5581 ggcccttgat ccccgcggct tcacccttca tgccacagat aaagccactg atgaagaacg
     5641 ggctcgcccg tttatgtggc ggttctggat aaaaacacct gcaaaaggac gcattgcatt
     5701 gtttgcccgg ggctggtaca gccgtgcact tgcagaaaaa cttgtcatta tcggctggaa
     5761 aagatcgtta aaagaagaga tcaattccat caccatcttt gagcgccagc tctgtgacag
     5821 tggcgtaatc atcataaagt tctttttaca tatcagcaag gacgaacaga aactaagact
     5881 gttagaacgg gagagaaatc cacttacagc gtggctggtt accccgagta tctgggattt
     5941 ccatcacaat tatgatactt accttcccct gatagacgag ttcattgaaa agaccgatac
     6001 tgcttatgct ccctggcata ttattgaagc cactgaccgg aaatatgcca ttttaaaggt
     6061 gtactcgcat ctcgtaaaaa accttgaaag gtatgttgcc gactataaag gggccgcagg
     6121 aaaaacaaaa caaaaaagta tccagaaacc tccgaaaaat ccagtacaga gaaaatcttc
     6181 ttcagccagt tcctattcaa aagaggagtg ccaggaactg ctgaacaatc tgcagattga
     6241 gatgcttgaa ctcaattacg tcctcttcaa gcggaagatc ccgttcatta ttgtctatga
     6301 agggtgggat gctgccggaa aaggaggcaa tatcacgcgt cttgcccggt acattaaccc
     6361 gctggggtat aatgtcgttc ctgttgcagc cccgaatgat tacgaaaaag aacaccacta
     6421 cctctggagg tttatccgtc attacccgag agcagggtat tttgccattt atgaccgcag
     6481 ctggtacggg cgggtgctgg ttgaacgcgt ggaaggtttc tgtgcggaat ccgaatggca
     6541 acgggcatac caggaaatca atgaaatgga acaggattat gtcgaaagca gcggaggcgg
     6601 aatggtaaag ttctggcttg agataagcaa ggaagaacaa ctcaaacgct tccaccagcg
     6661 cgagaaagat ccgctcaagc actataagat cactgaagag gactggagaa accgggaaaa
     6721 ttgggaccac tatgatgcgg cggtagatga gatgcttgca cggacaaaca cctcctatgc
     6781 accatgggtt gtcatagagt ctgacaataa atggtacgcc cggatcaagg ccgtcaaaga
     6841 agtcatccgc tatgcggaga aacttttata aagtttatta atcccccgta aatttaattt
     6901 ttatctgttt tgcggattta cttaatgtcg atctttttga atctgtagta tcagatctca
     6961 ctttttttca tataactcca tatgatcaca aaattcggca ttaattatag cagcatccgc
     7021 cagaatctga tatcgtgtgc ccttcttttc cgatcagaaa agattcagga ccacgcaatt
     7081 tcgaaagggt tatgaatatt aaaaagaatt cgaagctaat gtctgatgaa catcccagca
     7141 attctatatt tattctgaca attattgcga ttgttgtcct tggggcaata ttcttatata
     7201 ttttgaataa acc
//
LOCUS       DASRLV010000108        11542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_770350,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000108 DASRLV010000000
VERSION     DASRLV010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 11542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11542
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770350"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFC43_04335"
     CDS             <1..345
                     /locus_tag="VFC43_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30445.1"
                     /translation="PATGTTPGSQVSGSQTIKPTIAVTIPPTGVFVKVSYIGSFSGEY
                     GMNGAMVNVKNSGERVYEVVNATGNITASFSKLDGSTKHDITVELWKNGKSVTSAHNT
                     IPFGKVSINYNL"
     gene            complement(426..851)
                     /locus_tag="VFC43_04340"
     CDS             complement(426..851)
                     /locus_tag="VFC43_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6e"
                     /protein_id="HZK30446.1"
                     /translation="MVELKIVVSDPKTGRAYNVDASGGAAGAIVGKRIGDEIDAGAMG
                     LAGYKIQITGASDRTGIPAKKSLPGAGRKKLLMTKGVGFHPTMEGERRRKTIRSNEVT
                     ADFVQINARVTSYGDKTLEELFPKVEGEKAEKKAKPGRR"
     gene            complement(1004..2779)
                     /gene="infB"
                     /locus_tag="VFC43_04345"
     CDS             complement(1004..2779)
                     /gene="infB"
                     /locus_tag="VFC43_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HZK30447.1"
                     /translation="MAENPKIRTPIVCVMGHVDHGKTSLLDRIRGSSVVSSEVGAITQ
                     HIGATIVPIDAIRSMSGIMGKSLNIPGLLFIDTPGHHAFTTLRARGGALADMAILVVD
                     ISQGFQPQTIEALQILRNCRTPFVIAATKIDRIHGWRVFENESFLTSFAKQNERVKGD
                     IENKTYEIVGKLSELDFSADRFDRVSDFKRNLAIVPVSAHTGEGISDLLLVMIGLAQR
                     YMGEELALEVEGPGAGTVLEVKEERGLGTTLDVILYDGTLAIGDEIAVATQDNVIVTK
                     VRSLLKPRPMKEILVEDRFERVKSVVAAAGIKVTAPNLENVIAGSPFFVIRGNREEVE
                     AKIKKEMTEIHVKLADEGIVIKADTIGALEALCKELDGKGIGVMRAEVGPVSRHDLID
                     TETIKNPTYRVLLSFNTQILPDAAEIIKNPLYNQVKVFEGRVIYQLIDQYLAWRDEQK
                     RLMEKQRFEHIVMPAKIRLLPDCVFRQSNPAVVGVRVLGGKLRSDINLVKTDGKKVGH
                     LKSMQLRQESIREADAGLEVAISIEGVTVGRQLNVGDDLFVDVPERHVKVLEREMLKT
                     LNVSTQEILEEFTTMKRKGDPFWGK"
     gene            3033..3105
                     /locus_tag="VFC43_04350"
     tRNA            3033..3105
                     /locus_tag="VFC43_04350"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3067..3069,aa:His,seq:gtg)
     gene            3232..4038
                     /locus_tag="VFC43_04355"
     CDS             3232..4038
                     /locus_tag="VFC43_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZK30448.1"
                     /translation="MSQKKYVHGYSERESARLADQAQTLTGLLHDDTRYPEGSEVLEA
                     GCGIGAQTVILAKNSPGAHITAIDISADSLQRAECMAQKEGITNVTFRQGDIFHLPFE
                     QGIFDHIFVCFVLEHLTEPQRALEQLRPLLKEGGTITVIEGDHGSAFFHPEDPDARLA
                     IDCLVRLQQQAGGNALIGRQLYPLVAGVGYSDILVSPRMVYVDASRPGLVEGFTKLTF
                     TAMVEGVADEVHRKGLMTLDAWERGIAALYRTAEPDGVFCYTFFKATARK"
     gene            4272..4718
                     /locus_tag="VFC43_04360"
     CDS             4272..4718
                     /locus_tag="VFC43_04360"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZK30449.1"
                     /translation="MDITAQTETIVDTIKSMNRCWTAGWDEPAFRQYIHPDAVAIVPT
                     TPGRLEGQDAYVAGWRAFVEAAVIHEWRETDHRVRIYSGGKSAVVTYLFTITFVTGVQ
                     KQTMQGRDMVFLVKAGRKWQVVADQFSPDPIHPEVISGIKNPAPSA"
     gene            4776..5030
                     /locus_tag="VFC43_04365"
     CDS             4776..5030
                     /locus_tag="VFC43_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HZK30450.1"
                     /translation="MDTTIIVGYIAGTLTTISFVPQVIRTWKLKETKDFSLAMLLLFA
                     AGMLLWTAYGIWINSFPIIVANIVTFGLVLFLLWMKIKYR"
     gene            complement(5082..5696)
                     /locus_tag="VFC43_04370"
     CDS             complement(5082..5696)
                     /locus_tag="VFC43_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZK30451.1"
                     /translation="MFCPKCGKETDALGKFCQWCGADIVTTPPLTPMVAPEEEDEEPD
                     VGVYAGLGRRIVAFIVDFILILLLDTVAAAILGLVRGLQNLYFYGVQHASVDSLTTEG
                     SSAALFGSIIAAYGIVVVVIPWLYYAGFESSRSQATPGKLLMRIVVTDLEGNKPSFAR
                     VTLRHFAKFISVLIIFIGFLMIGLTKNHQGLHDRIAGCLVLLQD"
     gene            5889..6368
                     /locus_tag="VFC43_04375"
     CDS             5889..6368
                     /locus_tag="VFC43_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30452.1"
                     /translation="MKSTIILWVLLGLCLSALSAGCTSPTQPEIKPVETTALTPVTTI
                     PTSAVTGISTPLAVETLPIEQYVDINVEKQRPDATIHLLYNGGKGEIFVQNVMMRVTR
                     SDGTVEEKFLNDGTRKPKRGDELIIQGTKNVDQVAVFLTSAGKTYKIYDKPLVLPIY"
     gene            complement(6389..7468)
                     /locus_tag="VFC43_04380"
     CDS             complement(6389..7468)
                     /locus_tag="VFC43_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK30453.1"
                     /translation="MIIKHLSIIAKKEFKGLFNEKTILLAVLLQLFIALFSSFLMVGL
                     TSMYDPSSLSKYSSSRYSIAYAGNESPLLLSLQQSKDFRVYQMDLSTAVTALKERKLA
                     AVIYVPDTLPDASDPVKITLYTLQNDLQAAIVDVKLKDVFLKYEKNLREIRAGRLKEE
                     LVGIQIPKAGGGGDFYEFVYGLLIPLLVFMPAIIASALVIDLITEEYQHETLETLIST
                     PITFAEVVWGKVIACELLVPIQAGAWLILLMINGIVIQNAVLILILVAISSFVLILLG
                     TLTALHYRERTAAQFIFSTALVVVILFALAVPDNPLNLLTRLAVGTAAGEQWLVLGFT
                     LGIVLILSYVTHLYAERIARTHQTG"
     gene            complement(7465..9270)
                     /locus_tag="VFC43_04385"
     CDS             complement(7465..9270)
                     /locus_tag="VFC43_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK30454.1"
                     /translation="MNTLRDITTIARWEVKKSFSMISRDVLPLSIVLFVLLMLVTGFT
                     AQTGLHLQDGMYRVGVDDPKIAGLLAGDARFSVYLLDEQVLYANRNSFDLTIVRGNVS
                     SLGTDKANAAQKTLERDYSKYVNSVYNTETDLFAAYPLWIDSQSVKSELTFLSTQSGQ
                     YVSAAPRRTAPVPEGEIKNVPTPSPALEISQEDLRKELVQSNAKNTQISRYTEALSSS
                     SSMGTFKTPSQLSPPLPFDSIILVFVFVFPLYFTSQFFMMSIMNERIERKGEILLSTP
                     VRTSSIIIGKALPYFIGMLAICAGLTLYIKASPIIILPLIPVIFFFLANALLIGMLSR
                     SFKELSFISIFFSTVATSYLFFPSIFANVHVISLISPLTLIVLTIQGSAWTITDYLYS
                     TSLFWLTGAVLFYIAARNFKEERLFSEKKLVSRMREFLSEVLPEKYPFLSLFLLSGFS
                     IPFVFMVQMMCLVLFFNLPMPYSLVFLLLFAALVEEIAKGVGVYTIYSRDPSFLTWKN
                     LVLAGAATATGFLVGEKLLLFVTLAQISDSVFGSILFLSLGSLWLPLLLHFVCVLIVA
                     SSMKIWGRKGFVPGLAVAMIVHCLYNLYFILGWIG"
     gene            9534..10424
                     /locus_tag="VFC43_04390"
     CDS             9534..10424
                     /locus_tag="VFC43_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK30455.1"
                     /translation="MITARNLKKKFDTFTALDDVSFEFEDGEIFGIIGHNGAGKTTLL
                     KIISGLIAPTSGELYINDIDVVKNPVLLKETLGYLPEESRLYETMTAENYLAFFGEIY
                     GLSAHEIRVRSTQLFAALSLEPDGKKIGEFSKGMKRKAAIARSLIHDPKFLVYDEATS
                     GLDPMTSRFIADYLRTLKKEGKTIVLSAHNLYQVEAICDKVMILKRGKMVAFGNMHQL
                     RDQFGSLTYTIFFAIGDPGNLIGHAKPYRQEEGFFIWEAQDMKELNECTAIIKDSGGR
                     VEKIESRYPSLEEMLVKIGK"
     gene            10487..11023
                     /locus_tag="VFC43_04395"
     CDS             10487..11023
                     /locus_tag="VFC43_04395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZK30456.1"
                     /translation="MKKPPTPDQSQNTGKHQQGDIIVRKVRECDRDGIIAIFNYYAAT
                     SYAAYPEMPVNDRFFEFLREGTLAFYVLEHSQQVIGFGLIKPVLPFPPFMRTGMLTYF
                     LMPEFTGHGLGNLLIDRLTSDALKMGMTSLVATMASKNIPSIHFHVRNGFTEVGRLQN
                     AGLKFDELFDVVWMQKML"
     gene            complement(11160..11516)
                     /locus_tag="VFC43_04400"
     CDS             complement(11160..11516)
                     /locus_tag="VFC43_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HZK30457.1"
                     /translation="MGNYCRGDVVIAPVACEERGVIKTRPAVVIGTGDRGEVYVCPIS
                     SKPASDTHCVPVTLDDFSEGGLDLFGESYILTSRVFVIRNGAVMGKRGRLTSESVSSV
                     LTGIPQSLFPENEKQT"
BASE COUNT         2987 a         2813 c         2886 g         2856 t
ORIGIN      
        1 cccgccacag gcactacccc gggttctcag gtatcagggt ctcaaacgat aaagccgacg
       61 atagcagtta ctattccccc cacgggagta tttgttaagg tcagttatat cggttctttt
      121 agtggtgaat atggcatgaa cggtgccatg gtaaatgtga agaactctgg tgaaagagtc
      181 tacgaagttg tcaatgcaac gggaaatatt actgcatcct tctccaaact ggacggatca
      241 acaaaacacg atattactgt tgaactctgg aaaaacggga aatcggtaac atctgctcat
      301 aacacaatcc cgtttggtaa agtaagtatt aactataatc tatgagaatt ttctcaactt
      361 ttttatattc tgaaaacatc cgaaaataag aaaaaaacca ctcagaaaat taaaaaaaat
      421 tgatattatc ttcggccggg ttttgccttt ttctctgctt tctctccctc aaccttgggg
      481 aagagttctt ctaaggtttt gtcaccgtag gaggtgacgc gggcatttat ctgcacgaag
      541 tcagcggtga cctcgttaga ccggatagtt tttctccttc gctccccttc cattgtcggg
      601 tggaacccga caccttttgt cataaggagt tttttgcgcc ctgcgcccgg gaggctcttt
      661 tttgccggta ttccggtccg gtcggatgca ccggtgatct ggattttgta tcctgcaagt
      721 cccatggcac ctgcgtctat ttcatctccg atacgtttgc ccacaattgc gcctgctgcg
      781 ccgccacttg catccacatt ataggcacga ccggtctttg ggtctgatac aactattttt
      841 aactctacca ttgttatgct ccgtattgat tctcattaaa aaatccaagt aacggatttt
      901 tcacgtctct ggctgccggc aattatgcct acacatgaga agtacccatt ttattatact
      961 ggtacgctat taaatcctcg cgtcaggttt tacaggttaa cagttatttt ccccagaagg
     1021 gatctccttt ccgcttcatc gtcgtgaatt cctcaagaat ttcctgcgtg ctcacgttta
     1081 acgttttcag catctcacgt tcgaggacct tcacgtgccg ttccggtaca tccacaaaaa
     1141 ggtcatcccc gacattcagc tgtcgtccga cggtaacacc ctcaattgaa atcgcgactt
     1201 cgagcccggc atccgcttca cggatagatt cctggcggag ctgcatggat ttgaggtgcc
     1261 ctactttctt tccgtccgtt ttgacgagat taatgtcgct gcgtaatttt ccgccaagca
     1321 cccgcactcc gacaactgcc gggttgctct ggcggaacac gcaatcgggg agaagacgga
     1381 tcttcgccgg catcacgata tgctcaaagc gctgtttttc catcagcctt ttctgttcgt
     1441 cccgccatgc aagatactgg tcgatcaact ggtagattac cctcccctca aatactttaa
     1501 cctggttgta aagggggttt ttgatgattt cagcagcgtc aggaaggatc tgggtattaa
     1561 aagagagaag tacacgatac gtcgggtttt tgatcgtttc tgtgtcaatc aggtcgtgac
     1621 ggctcaccgg cccgacttct gcccgcataa ccccgattcc ctttccatcc agttccttgc
     1681 agagtgcttc gagagccccg atggtatctg ccttgatcac gataccttca tcagcaagtt
     1741 ttacgtggat ctccgtcatc tcttttttta tttttgcttc aacttcttcc cggttcccgc
     1801 ggattacaaa aaatggcgac cctgcaatca cgttctcgag gtttggtgca gtcactttaa
     1861 tccccgctgc tgcgactact gactttaccc tctcgaacct gtcctcgacg aggatctctt
     1921 tcatagggcg gggttttaac agcgaacgca cttttgttac tatcacatta tcctgcgttg
     1981 caacggctat ctcgtcgcca atcgccagtg tcccgtcata gagaatcaca tccagggttg
     2041 ttccgagccc gcgttcttct ttcacttcaa gaactgtacc ggctccggga ccttcgactt
     2101 caagtgcgag ttcctcaccc atataccgct gggccagccc gatcatcaca agcaggagat
     2161 ctgaaatacc ttcacctgtg tgggcgctga ccgggactat tgccaggtta cgcttgaaat
     2221 cagacacccg gtcaaacctg tctgctgaga agtccagttc agagagcttt cccacaattt
     2281 cgtatgtttt attctcaata tcccctttca ccctctcgtt ttgtttggca aacgaggtga
     2341 ggaacgactc gttctcgaac acccgccagc catggatccg gtcaattttg gttgcagcaa
     2401 tgacaaacgg tgtccggcag ttcctcagga tctgaagtgc ttcgattgtc tggggctgga
     2461 agccctggct gatatcgaca acaaggatag ccatatctgc gagtgcccca ccgcgtgccc
     2521 ggagggtggt aaatgcgtgg tgtcccgggg tgtctatgaa taacaggccg ggaatattga
     2581 gggattttcc cataatcccg ctcatggatc ggatggcatc gatgggaacg attgttgcgc
     2641 caatatgctg ggtaatcgca cccacctcgg aactgactac ggatgaaccg cggatacggt
     2701 ccagaagaga ggtcttcccg tgatcaacat gccccataac acagacaatc ggcgtcctga
     2761 tcttcgggtt ttccgccatt tcactatccc cttttccctg ctctggtacg aaaatatgta
     2821 accaattcct cactggcacc gtcgtctgga atgccatgcc gcatgattaa ggaattgagt
     2881 tcagagccac ctgttatgta atggacaccc gaccctatta ataatcacta tatgacttca
     2941 ggcaatcgaa aaaaaactgg gatgtcgttt cagacgattg atgcacaaag atacattaat
     3001 cagagtccgg tgcaaatgta aaagggacat ttgccagggt agggtagttg gttatcctag
     3061 aggactgtgg atcctccgac ccgggttcga atctcggccc tggccttttc ttttatctta
     3121 cttattatta caaacgctta agatgcacaa tagtgttgct ttactaaaaa ggaaggatag
     3181 tcatggttta taatcgggcc catgcatttt taatgacctt cagggttttt catgtcacaa
     3241 aaaaaatacg tccacgggta ttcagaacga gaatcggcaa ggcttgctga tcaggcacag
     3301 accctgacgg gccttctgca tgatgatacc cggtaccctg aaggctcgga ggtgctggaa
     3361 gccggctgcg gtatcggggc acagacggtt atacttgcaa aaaacagccc cggtgcacat
     3421 atcaccgcga tagatatatc ggccgactca cttcagcggg cagaatgtat ggcccaaaag
     3481 gaaggcataa ccaatgtgac cttccgccag ggagatattt ttcatctgcc gtttgagcag
     3541 gggatattcg atcacatctt tgtctgtttt gtgctggagc atctcaccga gccacagcgt
     3601 gctctggagc agctccgtcc actcctgaag gagggaggga cgatcacggt tattgaagga
     3661 gaccacggct ccgccttctt ccacccggaa gacccggatg caaggctggc catcgattgt
     3721 cttgtcaggc tccagcagca ggccggcggc aatgccctga tcggcaggca gctctacccg
     3781 ctggtggccg gggtgggata ttcggatatc cttgtctcgc cgcggatggt ctatgtcgat
     3841 gcctcccgcc ccggccttgt tgaaggattc acaaagctga ccttcacggc aatggtggaa
     3901 ggcgttgctg atgaagtgca ccggaagggt ctgatgaccc tggatgcatg ggagaggggg
     3961 attgcggcac tctaccgtac agctgagccg gacggggtct tctgctacac cttcttcaag
     4021 gccaccgcga ggaaatgaga gggcccctga agatttcctt tgtccttgct caggtaccag
     4081 gcttttaccg gaggatgttt ggtgttgttg tcaccagcac ggaagatcag cgcagggaat
     4141 ggctcggtga ggtccgcaaa agggttttaa agatatttcc acccgggccc cgctgaccgt
     4201 tccggatact ttcgcaccct gcacccctgc tctttttacc tgatttcatg cgtaggtata
     4261 ctggaggaat tatggatatc actgcacaga cagagaccat tgtagacacg atcaaatcca
     4321 tgaaccgctg ctggactgca ggatgggatg aaccggcatt ccggcagtac atccatcccg
     4381 atgcagtagc catcgtcccg acaactcccg gaaggctcga aggacaggat gcctacgttg
     4441 ccggctggcg ggcgtttgtt gaggcggcag tcatccacga atggagggag accgaccata
     4501 gggtgcgtat ctatagcggg ggtaaaagtg cggtggttac ctatctcttc accatcacct
     4561 ttgtgaccgg tgtgcaaaag cagaccatgc agggcaggga catggtattt ctggtaaaag
     4621 ctgggcggaa gtggcaggtg gttgcagacc agttctcacc cgatccaatt catcctgaag
     4681 tcatttccgg catcaaaaac cccgcgccgt ctgcctgaac gaaaatccga ccggttttaa
     4741 atggtcggat gaaaaatact aggaggaggt atttcatgga tacaaccatc atcgtcggct
     4801 atatagcagg gacactcacc acaatctcat ttgtcccgca ggttatccgg acctggaagc
     4861 ttaaagaaac caaggatttt tcacttgcca tgctcctgct gtttgcagca gggatgctcc
     4921 tctggacagc gtatggtatc tggatcaact cgtttcctat catcgttgca aatatcgtaa
     4981 cgtttggtct cgtgctgttc ttactctgga tgaagatcaa ataccggtaa tatccttatt
     5041 tcgggtgtct ccggcaaaaa aaatataaaa aaaaaagttt cttaatcctg aagaaggaca
     5101 aggcatccgg caatcctgtc gtggagcccc tggtgattct tggtgagccc gatcatgagg
     5161 aatccgataa agataatcag aactgagatg aattttgcaa agtgccgaag ggtaacgcgt
     5221 gcaaaggatg gtttgttccc ttcaagatcg gtcacgacaa tacgcatcaa caatttaccg
     5281 ggggtggcct gactgcggga cgactcgaat cccgcataat acagccacgg tatgacgacg
     5341 acgactatcc cgtatgcggc aatgatggat ccaaataaag ccgcagatga cccttccgtt
     5401 gtaagtgagt caacggatgc atgctggacc ccataaaaat agaggttctg caatccccgg
     5461 acgagaccca gaattgcagc tgcaactgta tcaagcagca gaataagaat aaaatctaca
     5521 ataaaagcga caatccgcct gccaagacca gcatatacgc caacatcggg ctcctcatcc
     5581 tcctcttcag gagcgaccat gggtgttaac gggggggttg ttacgatatc cgccccacac
     5641 cattggcaga attttcctaa agcatcagtc tcttttccac attttggaca gaacattccc
     5701 ttttactcct gatatgtaaa gttaatccgg gtgtcctttt taatttaact gttcttttct
     5761 gctatttcat gattttatgc tgctttaaac cgcgtactgc gggaaaaatc ctgtacatct
     5821 ggacaaatgg ttatttgcac gattcacgag cgcaaatcag aaatataaaa aaaaataaag
     5881 cactcttcat gaaaagcacc ataattctgt gggtgttgct cgggctttgc ctcagtgcgc
     5941 tctctgcagg ctgcacaagc ccgacacagc cagaaattaa accggttgaa acaacggctc
     6001 tgacccccgt cacgacaatt cccacatcag cggtgaccgg aatatcgaca ccacttgctg
     6061 ttgaaaccct tcccattgaa caatatgttg atatcaatgt cgaaaaacag cgacctgatg
     6121 ccacgattca cctcctctat aacgggggaa aaggagagat ctttgtccag aatgtcatga
     6181 tgcgggttac ccgttctgac gggacggttg aagaaaagtt cttaaacgac ggaaccagaa
     6241 agccaaaacg tggtgatgag ctgattattc agggtaccaa aaacgttgac caggttgcgg
     6301 tttttcttac aagcgccggt aaaacgtaca agatctatga taagcctctt gttttaccaa
     6361 tctactgaac aatctttttt ttcccggatc aacccgtctg atgcgttctg gcgatccttt
     6421 cagcgtacag gtgcgtcaca tagctgagga tgagaacaat tccgagggta aatccaagga
     6481 caagccattg ttcccccgcc gcagttccga cagcgagccg ggtcagcagg ttaaggggat
     6541 tatccggcac tgccagggcg aaaaggatca caacgacaag tgcagttgaa aagatgaatt
     6601 gcgccgctgt tcgttcacgg tagtgcagtg cggtcagtgt gccaagaagg attaagacaa
     6661 atgaagagat cgctacaagg atgagtatca aaacggcatt ctggatcacg ataccattga
     6721 tcatgaggag aatcagccag gctcctgcct gtatcggaac aagcagctcg caggcaatca
     6781 cttttcccca tacaacctcg gcaaatgtaa taggtgtcga gataagggtt tcgagcgtct
     6841 cgtgctggta ctcctcggta atcaggtcga tgacaagtgc tgatgcgata attgccggca
     6901 taaagacaag gagagggatc agcagaccgt acacaaattc ataaaagtcc ccgcctccgc
     6961 ccgcttttgg aatttgaatc cctacgagtt cttctttcag acggccagca cggatttccc
     7021 gcaggttctt ttcatatttg agaaaaacat ccttgagctt gacatcgaca atagctgcct
     7081 gaagatcatt ttgcagggta tacaaggtta tcttaacggg atctgatgcg tccggcaggg
     7141 tatccggcac gtagattacc gcggcgagtt tgcgttcttt gagagctgtt acggctgttg
     7201 aaagatccat ctgatagacg cgaaaatcct tactttgctg aagagaaagg agtaatggtg
     7261 attcatttcc tgcataggca atgctgtacc gggaactgga gtattttgag agtgaggacg
     7321 gatcatacat cgaagtcagg ccgaccatca ggaatgagga gaacagggcg atgaagagct
     7381 gtaataaaac ggcaagcagg atcgtttttt cgttaaacag ccccttgaac tccttttttg
     7441 caatgattga caggtgtttg attatcatcc gatccacccc agaatgaaat aaaggttata
     7501 caggcagtgc actatcatgg caacggcgag gcccggaaca aatccttttc tgccccaaat
     7561 cttcatgctg gaggcaacga taagcacgca gacgaagtga aggagcaagg gaagccagag
     7621 ggaccctaac gataaaaaga ggatactgcc aaacaccgaa tctgagatct gtgcgagggt
     7681 aacaaagagc aggagtttct cgccgaccag aaatccggtt gctgttgcag caccggccag
     7741 cacgaggttt ttccaggtga ggaatgaagg atcgcgggaa tagatcgtat acacgccgac
     7801 ccccttggca atctcttcga ccagcgcagc gaagaggagc aggaatacca gcgagtaggg
     7861 catggggagg ttaaaaaaga gcacgaggca catcatctgg accatgaaga caaacggtat
     7921 ggagaatccg cttaacagga agagcgaaag aaacggatat ttctcaggca ggacctctga
     7981 gaggaattct ctcatgcggg ataccagttt cttctctgaa aagagccgtt cttccttgaa
     8041 atttcttgca gcaatataaa aaaggacagc tccggtgagc cagaacagag atgttgaata
     8101 gagataatcg gttatcgtcc aggcagagcc ctggatggtc aggactataa gggtaagggg
     8161 ggagatgagg ctgatgacat ggacattggc aaagatactc gggaaaaaga ggtaggaagt
     8221 tgccacagtc gagaaaaata tcgagataaa ggagagttcc ttgaaactgc gcgaaagcat
     8281 gccgatgagt aacgcgttag ccaggaaaaa gaagatgaca gggatgagcg ggaggataat
     8341 tattggcgat gccttgatgt acagggtaag cccggcacat attgccagca tgccgataaa
     8401 atacgggaga gccttgccga taattatcga cgaggtccgg accggtgtgg aaaggaggat
     8461 ctcgcctttt cgttcgatgc gctcgttcat tatgctcatc ataaaaaact gggaggtgaa
     8521 gtagagcgga aagacaaaga caaagacaag gatgatggaa tcaaagggca ggggaggaga
     8581 gagctgggac ggcgtcttga aagtccccat cgagctgctg ctggagagtg cttcagtata
     8641 ccgggagatc tgggtgttct ttgcattcga ctggaccagt tcctttctca ggtcctcctg
     8701 tgagatctcg agcgcaggcg aaggggtcgg aacatttttg atttccccct ccggaacagg
     8761 tgctgtccgc cgcggggcag cgctcacgta ctggccgctc tgggttgaaa gaaaggtgag
     8821 ttcgcttttc acactctggc tatcgatcca gagcgggtat gccgcaaaca ggtcggtttc
     8881 agtgttatag acactgttca catacttcga ataatcgcgt tcaagtgttt tttgtgcggc
     8941 atttgctttg tcggttccca ggctggacac attcccgcgg acaatggtaa gatcaaatga
     9001 attcctgttc gcatacagga cctgttcatc aaggagatac acagaaaatc tcgcatcccc
     9061 agccagaagc ccggcaatct tcgggtcatc gactcccacc cggtacattc cgtcctgaag
     9121 gtgcaaccct gtctgtgcgg taaaaccggt taccagcatc agcaggacaa aaaggacaat
     9181 ggaaagtggc agcacatccc ggctgatcat ggagaaggat tttttcactt cccaccgggc
     9241 tatggtggtg atgtcacgta gcgtgttcat gatatgtcct ttcgcaggtt ggatgcattg
     9301 ctgccatggc cagatttttc cttaccatcc gtatgggtga tagttgagta cagagatgta
     9361 tagagtatcg gtcaaataat catcggttct gacaatctct caggtaccgg ccattaccct
     9421 gcaagaggat atcagacggt gaatgcaaag tccatccggg ccggttggag ctgtgccagc
     9481 aacctaaggt atatgtttgt acccgggcaa tcagatcgat aaggtatcat ctcatgatta
     9541 ctgcccgcaa cctgaaaaaa aagttcgaca cttttactgc actggacgat gtgagttttg
     9601 agtttgagga tggtgagatc ttcgggatca tcgggcacaa cggagcggga aagacaacac
     9661 ttctcaagat tatatccggc ctgatcgcgc caacatccgg ggagttatat atcaacgata
     9721 tcgatgtggt gaaaaacccg gtactcctga aagaaactct cgggtatctc cctgaggagt
     9781 cccggttgta cgagacgatg accgctgaga attatctggc tttttttggt gagatatacg
     9841 ggttgtcggc acatgagata cgggtcaggt caacccagct gttcgcggcc ttgtcacttg
     9901 agcccgatgg caagaagatc ggggagttca gcaagggcat gaagcggaag gccgctatcg
     9961 cccggtcgct catccacgat ccgaagttcc tggtatatga tgaagcaaca agtggtctcg
    10021 atcccatgac ctcgcggttc attgccgatt acctgcgcac gctcaaaaag gaaggtaaaa
    10081 cgatcgtgct ctcggcacac aacctttacc aggtcgaggc gatctgtgat aaggtgatga
    10141 tcctgaaacg cggcaagatg gtcgcctttg ggaatatgca ccagctccgc gaccagttcg
    10201 gttctcttac ctatacgatc ttctttgcta tcggagatcc cggcaatctg atcgggcacg
    10261 caaaacctta ccggcaggaa gaagggttct tcatctggga agcgcaggac atgaaggaac
    10321 tcaacgaatg caccgcgatc attaaggatt ccggaggaag ggtggaaaag attgagtccc
    10381 ggtacccttc actggaagag atgctcgtaa agattgggaa atgatctgat caataccgat
    10441 ttgccaatct gaccgacgtg ggaatcttaa caaaagttga aagaatatga aaaaaccccc
    10501 cacaccagat cagtcacaga ataccggaaa gcaccagcag ggagatatta tcgtaagaaa
    10561 ggtgcgtgaa tgtgaccgcg acggcattat agctattttc aattactatg cagcgaccag
    10621 ttatgctgca tatcccgaaa tgccggtaaa tgatcgtttc tttgaatttt tacgtgaagg
    10681 aacccttgcg ttctacgttc ttgaacattc acaacaggtg attggatttg ggttaatcaa
    10741 gccggtactg ccatttcctc ccttcatgag gacaggcatg ctgacctatt tcctcatgcc
    10801 ggaattcacc gggcatggtc ttggaaatct gcttattgac cggcttacgt ccgatgcatt
    10861 aaagatgggg atgacctcgc tggttgcaac tatggcctca aaaaatatcc cgagtatcca
    10921 ctttcatgtg cggaatggat ttactgaagt aggcaggctg cagaatgccg gcttgaaatt
    10981 cgatgaacta ttcgatgtag tctggatgca gaagatgctg tgatagtgtc agcggctctg
    11041 gtaacccatg aatcattttt cacaaacaga gcctaaatcc tgaccgtgct ccctgtttca
    11101 ggcagagata aagtatagct aaaattgata ctattatcgc agggttcgtg aacctttttt
    11161 taagtttgct tttcgttttc gggaaacagc gattgaggta tcccggtcaa aaccgatgag
    11221 acagattcgc tggtcaggcg cccccgtttt cccatgactg caccattccg gatcacaaaa
    11281 acccgcgatg tcaggatata actttcgcca aaaaggtcaa gacccccttc tgaaaaatcg
    11341 tcaagagtga cagggacaca gtgagtatcg gatgctggct tactgctgat cgggcagaca
    11401 tagacctcac cacgatctcc tgttccaatc accactgcag gacgggtctt gatcacaccc
    11461 ctctcctcgc aagcgacagg ggcgatcacc acatcgcccc ggcaataatt tcccatgcat
    11521 acctagtcag gtgtataaaa gc
//
LOCUS       DASRLV010000109         2948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_601779,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000109 DASRLV010000000
VERSION     DASRLV010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601779"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VFC43_04405"
     CDS             complement(<1..514)
                     /locus_tag="VFC43_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZK30458.1"
                     /translation="MKQILITGDRSGSGKTSITLALSALLSQKNRVQTFKVGMDYIDP
                     SYLSAVSKRPCRNLDSFTLSDTQIRDIFQYGCRDADIAIVEGVRGLYEGAEALDDIGS
                     TASVAKALNLPVVLVVSAQSITRSAAALVKGFQEFDRDVHIAGVILNNIKGGSHKEKA
                     VTAIEHYCGIP"
     gene            complement(511..1575)
                     /gene="cfbD"
                     /locus_tag="VFC43_04410"
     CDS             complement(511..1575)
                     /gene="cfbD"
                     /locus_tag="VFC43_04410"
                     /EC_number="6.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide reductive
                     cyclase catalytic subunit"
                     /protein_id="HZK30459.1"
                     /translation="MKYMHPRPSSIVAALYTARDLKVDVAILHGPSGCSFKHARLLEE
                     DGMRVLTTSLADNEFIFGGQKPLEDVLRYAEDHFSPARMAVIGTCVAMIIGEDLQSAI
                     DGAGVTTPTIAVDIHAGYLENIAGVLSTLEAACEAGWISTDELTRQRFLMEKANEVER
                     IRGAASLPYIEPSRGDLKHITARQLLDLARSGKKGVAILNAKKETAYMFADELVALHD
                     TCPDADITYIANLEQRGLPKTRLDAERILKEMKSRGIKIELLGALDEYGANGDLLGAK
                     LSEISPRFALISGVPHAIPPAYTQGIECFSVTNGPRQVGPLRAIGHQHVIVEIDLHPK
                     TLGVTTIIESEFGAIMRSLV"
     gene            complement(1586..2806)
                     /gene="cfbE"
                     /locus_tag="VFC43_04415"
     CDS             complement(1586..2806)
                     /gene="cfbE"
                     /locus_tag="VFC43_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F430 synthase"
                     /protein_id="HZK30460.1"
                     /translation="MHILVLDTIHGGKIIGEAYTARGENVDCVDVYRKESSMDVKTAQ
                     SRNYDLVVAPVHLDPDHPLIKFATAPVISHHEAIRRLLGKNIPEPMVEITGSRGKTTT
                     AHALASLMKGPGILHTSTGTFRFPEKELISRSSITPGSVLAAVRMALDINGWLIVEES
                     LGISCAGTLGIITSSEDYSFATGKKSALQSKIASTKNCRQVLLAEKISSPSFKGVVHI
                     EDVASVKDTECSLSLSSQTCRFSNPLLLLPVYRNSLVLAAAAAMMLGLNPELLSSFRA
                     LSGRMSESYEQDVLVVDNANSGTNLAATVEAAQYARELAGREAITLVIGQVKGDGAVC
                     EGFSFEQICEAINQVRPAHIVWVGTVPQPKTAEFTKLEPLISAYEVTLEEARTAALRM
                     ATQGSIVLAVKTWR"
     gene            complement(2816..>2948)
                     /locus_tag="VFC43_04420"
     CDS             complement(2816..>2948)
                     /locus_tag="VFC43_04420"
                     /EC_number="4.99.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106968.1"
                     /note="type II chelatase; cobaltochetalase; functions in
                     cobalamin (vitamin B12) biosynthesis by delivering the
                     correct metal; certain archaeal proteins form a shorter
                     version (CbiX(S)) as compared to the longer version in
                     bacteria (CbiX(L)) which may have resulted from a
                     duplication of the original cbiX(S) gene; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sirohydrochlorin nickelochelatase"
                     /protein_id="HZK30461.1"
                     /translation="GTKKGVFSLNGRSVPLVYADPIGSDPLLASLMIKNAAKALTLL"
BASE COUNT          702 a          752 c          714 g          780 t
ORIGIN      
        1 ccgggatacc gcaatagtgt tcgatggcag taactgcctt ttctttatgg gaacctcctt
       61 tgatattatt caggatgact cctgcaatat gtacatcccg gtcaaattcc tggaatcctt
      121 tcaccagcgc agcagcactg cgggttatac tctgtgccga aaccacaagc acgaccggaa
      181 ggttgagggc ctttgcaaca gatgccgtgc ttcctatatc gtcaagcgcc tctgctcctt
      241 catataaccc gcggactcct tcgacaatag cgatatctgc atcacggcag ccatactgga
      301 agatatcgcg tatctgggta tctgaaagtg taaaactgtc aagattccgg cagggccttt
      361 ttgagactgc ggaaaggtaa gaaggatcaa tatagtccat cccgacttta aatgtctgaa
      421 ccctgttttt ttgggaaagg agtgcggaaa gggcaagggt gatgcttgtc ttgccgctac
      481 cggaacgatc accggtaatc aggatttgtt tcataccaga ctcctcatga tggcaccgaa
      541 ttcgctctcg ataatggtag tgacccccag tgtcttgggg tggaggtcga tttccacaat
      601 cacatgctgg tgaccaattg ccctgagagg acctacctgt cggggaccat ttgtcactga
      661 gaagcattca ataccctgtg tgtatgccgg aggtattgca tgcgggacac cactgatgag
      721 agcgaatcgg ggtgaaattt ctgacagttt cgcgccgagc agatcaccat tcgccccata
      781 ttcatcgagt gccccgagaa gttcgatctt tattccccgg ctcttcatct cttttaagat
      841 ccgctccgca tccagtctgg tcttgggaag accccgctgt tcaagattgg caatatatgt
      901 gatatctgca tccgggcagg tatcatgaag agcgacaagc tcatcagcga acatataagc
      961 cgtctctttc tttgcattca ggatggcaac accctttttt ccactccggg caagatcgag
     1021 gagttgtcgg gcagtaatat gtttcagatc accccgtgag ggttcaatat agggaagact
     1081 cgctgcaccc cttatccgtt ccacttcatt tgccttttcc atcaggaacc gttgcctcgt
     1141 gagctcatct gttgagatcc agccagcttc acatgcagct tccagtgtcg aaagtacccc
     1201 cgcgatattc tcaagatacc cggcatgaat atcaaccgcg attgtaggag ttgttacgcc
     1261 ggctccatcg atcgccgact ggagatcttc accgattatc atggcaacac aggtccctat
     1321 cactgccatt ctcgcgggtg aaaaatgatc ttcggcgtat ctgaggacat cttccaaagg
     1381 tttctggcct ccaaaaataa attcattgtc tgcaagtgac gtcgtgagta cccgcatgcc
     1441 atcctcctcc agcagccgtg catgtttaaa cgaacagccg gatggaccgt gaaggatggc
     1501 cacatcgact ttgaggtcac gggcggtata gagtgctgca acaattgaac tgggtcgcgg
     1561 gtgcatgtat ttcattatcg atcagttatc tccaggtttt tacggcaagt acaattgatc
     1621 cttgtgttgc cattcgcaaa gctgcggtac gggcttcctc cagggttact tcatatgcgg
     1681 atatgagcgg ctccagtttt gtgaactccg ctgttttggg ttgcgggact gttccgaccc
     1741 atacgatatg ggctggacgt acctggttga ttgcttcgca tatctgttca aaagaaaatc
     1801 cttcgcagac cgcaccgtca cctttcacct ggccgatcac cagtgtaatg gcttctcttc
     1861 cggctaactc ccgtgcatac tgtgctgcct ccacagtcgc agccagattc gttccgctgt
     1921 ttgcattgtc tacaaccagc acatcctgct cataggattc cgacatcctg cccgaaagcg
     1981 ccctgaagga tgataataac tcagggttca ggccaagcat cattgcagcg gcggcagcaa
     2041 gtaccaggga attccggtat acggggagca aaaggagcgg attagaaaac ctgcaggttt
     2101 gtgatgagag cgacagggag cattctgtat ccttaactga tgctacatct tcaatatgca
     2161 caactccctt gaatgatgga gatgagattt tttctgccaa aaggacctgt ctgcagtttt
     2221 ttgtcgaagc aattttactc tgaagtgctg acttttttcc tgtggcaaag gaataatctt
     2281 cagaagacgt gataatccca agggttccgg cgcagctgat tcctaatgac tcctccacga
     2341 tgagccagcc attgatatca agtgccatac ggacagcagc aagtaccgat cccggtgtaa
     2401 tactgctccg ggatatcagt tccttttccg ggaaccggaa tgttcctgtc gatgtatgca
     2461 gtatacccgg tcctttcatc agggatgcca gggcgtgtgc tgtggtagtc tttccccttg
     2521 aaccggtaat ctcaaccatg ggttccggga tattctttcc cagcaggcgg cgtatcgctt
     2581 catgatggga aatcaccggt gctgtggcaa attttatgag aggatgatcc ggatcaaggt
     2641 gaaccggggc taccacgaga tcataattcc gtgactgagc agtttttaca tccatggagc
     2701 tctctttgcg gtaaacatca acacaatcaa cgttctcgcc ccgggccgta tacgcttcac
     2761 ctattatttt tccaccatgg atggtgtcca gtaccaggat atgcataaaa aaaagttaca
     2821 ggagagtgag tgcttttgct gcattcttta tcatcagact ggcaagcagg gggtcgctcc
     2881 ctataggatc tgcatacacg agagggacag atcttccatt gagtgaaaaa acacctttct
     2941 ttgtaccc
//
LOCUS       DASRLV010000110         4084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1170908,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000110 DASRLV010000000
VERSION     DASRLV010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170908"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..1084
                     /locus_tag="VFC43_04425"
     CDS             125..1084
                     /locus_tag="VFC43_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HZK30462.1"
                     /translation="MQDYLQVFADWVTYTILGLVQGSPLASSVNFFVYDSIKVTLMLA
                     VIVFGVAIIRSFITHQKVKKWVAGRTEGAGNVLAALLGIPTPFCSCSAVPLFIGFVES
                     GVPLGITFSFLVASPLINEVAAAMLLAMFGWKIALIYIVSGLFIAIIAGIIIGRLHLE
                     SEVEEFVWKCKMHDQPDKAMTWKDRIDFGISESKAITVKVLPYILAGIGIGAVIHGYA
                     PADFLVNIAGPNNPLAVPIAVLIGVPLYSNAAGMIPIMEVLTAKGMAMGTALAFMMAV
                     IGLSLPEMIILRKVIKIKLLVIFAGILFVSFTLTGYLFNAILG"
     gene            1110..1544
                     /locus_tag="VFC43_04430"
     CDS             1110..1544
                     /locus_tag="VFC43_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HZK30463.1"
                     /translation="MKKSDEALARFRNGFTCSSAVFSAFSDELGLDSDTAKKIACGFG
                     AGISKTGNICGAVSGAIMVIGLKYGKTTQGDDAATEKTRAMVRTFMLEFTKRHGSVCC
                     TELLGYNLGKTDEYEKARDSKLFVTKCPELVADAAAILKIIL"
     gene            1637..2680
                     /locus_tag="VFC43_04435"
     CDS             1637..2680
                     /locus_tag="VFC43_04435"
                     /inference="COORDINATES: protein motif:HMM:NF024981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance protein"
                     /protein_id="HZK30464.1"
                     /translation="MTNDSARLGWVEKLLALWIFLCIAIGLALGKYFPEFSQHLAIGI
                     PIGLFLMIYPAMTKIELGELKDALKSKKQVGIIVFFNYLVNPFLLYIIGYIFFEKILL
                     GLGWITPEAASYLWTGLILLGVAPCIAMVLVWTDLAKGNGPLGIVLMAWNSIIQVLTT
                     PFYIALLVGTYIAIDIFLIGQSVLLYLGLPLLFGVITRREVIKRKGVEWLNTRLIPRF
                     NALQLLALLFTMVVMFSLKGGVIIDNPNLIWQMAIPVVLFFFLLFHIVYYTTRRMGYN
                     YEDSATMGFHCTGRNFELAIAIALTAFASMPMVAVSTVVGPLIEIPVMLGIVWLSLKR
                     RDRIMQQGKAYLS"
     gene            complement(2755..2949)
                     /locus_tag="VFC43_04440"
     CDS             complement(2755..2949)
                     /locus_tag="VFC43_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30465.1"
                     /translation="MPYPLFLSQKGTCTGDISTGTSTRGPITVANATGEAMPITAIAT
                     AIASSKLLDAAVNESEADFW"
     gene            3081..>4084
                     /locus_tag="VFC43_04445"
     CDS             3081..>4084
                     /locus_tag="VFC43_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZK30466.1"
                     /translation="MVIIMDGYAGNIVYADLSAGTVTIRPTPPELKKMYIGGRGFGIR
                     LLTDMVDPKIDPLGEKNVVVYAAGPLTGTGIPLGSRYEVSTISPLTGTAMSANSGGVF
                     GWKMKKAGFDGVVVSGKAKKPVYLLLDNGKAEIRDAAHLWGKTTGQTTDAITDELKDT
                     SVRVSCIGPAGEKLVLFACIINEKTRAAGRGGVGAVMGSKNLKAIAARGDLPIKPADE
                     EKLNIVKDRVRKKIEDNGIEKALHNYGTAVLVNIVNENYVLPTRNFQTAHFADAEMVS
                     GETIAKTILKKPKGCYACIVQCGRVHEFDGETGEGPEYEPDWAFGPDCGINDLKAVVR
                     A"
BASE COUNT         1004 a          983 c         1063 g         1034 t
ORIGIN      
        1 gctgatattc acctggtggc tacagaactc ggggttgaga aaaacggaat ggccgaaatt
       61 cagtggaacg acgttgaaac agtgctttca gcccttgaac agagaataaa cggagtataa
      121 aaccatgcag gattatttgc aggtgttcgc cgactgggtt acctacacga tcctcggtct
      181 ggtgcagggc agtccccttg catcctcagt gaacttcttc gtatacgact caatcaaggt
      241 aacgctgatg ctcgctgtta tcgtcttcgg cgttgccatc attcgttcct ttatcactca
      301 ccagaaagtg aagaaatggg tagccggccg tacagaaggg gcaggtaatg ttcttgcagc
      361 tctccttggt atcccaaccc ctttttgctc ctgctctgca gtgcctctgt ttattgggtt
      421 tgtggaatcc ggggtaccac tggggatcac attctcgttt cttgtcgcat cacccctcat
      481 caatgaggta gcagcggcaa tgcttcttgc catgttcggc tggaagatag cattaatcta
      541 tatcgtttcc ggcctgttca tcgctatcat tgccggcatt attatcgggc gacttcacct
      601 tgaaagtgag gtcgaggagt ttgtctggaa atgcaagatg cacgaccagc cggataaagc
      661 aatgacatgg aaggacagga tagacttcgg catcagtgaa tccaaggcta ttaccgtaaa
      721 agtgcttccc tacatcctgg cagggatcgg gataggtgcg gttatccatg gttatgctcc
      781 tgccgatttc cttgtcaata tcgcaggtcc gaacaatcct ctcgcggtcc ccatcgcagt
      841 gctgatcggt gtcccgctct actcgaacgc cgcagggatg atacccatca tggaagtgct
      901 cacggcaaaa gggatggcga tgggcaccgc gctcgcattc atgatggcag tgatcgggct
      961 ctcacttccg gagatgatca tcctccgtaa ggtgattaaa atcaaactgc ttgtaatctt
     1021 tgcagggatc ctgttcgtgt ccttcacgct gacaggatac ctgtttaatg cgattctggg
     1081 ataatatact acccgacagg agaaacacca tgaaaaaatc tgatgaggcg ctggcccgtt
     1141 tcaggaacgg gtttacctgt tcttcggctg tcttttccgc attctctgat gaactcggac
     1201 tggatagtga taccgcaaag aagatcgcct gcggttttgg ggcgggtatc tccaagaccg
     1261 ggaatatctg tggtgcggtg tccggggcaa tcatggtgat cggtctgaaa tatggaaaaa
     1321 caacgcaggg tgatgatgca gccactgaga agacaagggc gatggtccgc acattcatgc
     1381 ttgaattcac caaacgccat ggttcggtct gctgcacgga actgcttggc tataatctcg
     1441 gcaaaacgga tgagtatgaa aaagcccgtg acagcaaact gttcgtgacg aaatgtccgg
     1501 aacttgtcgc agatgctgct gcaatcctta aaataatcct ttaaaaaggg tgaccaggtg
     1561 accccgatga gacgaccaaa aaaacatatc tcgtttcctg attggtacct gaaattttaa
     1621 acggagttgg agtaagatga caaatgattc tgccagactg ggctgggtcg agaaactgct
     1681 ggccctatgg atatttctct gcatagcgat cggactggct ctcgggaagt acttcccgga
     1741 attcagccag catctagcga tcggtatccc aatagggtta tttttaatga tctatccggc
     1801 gatgacaaag atcgaactgg gagaactcaa agacgccctt aagagcaaga aacaggttgg
     1861 catcatcgtt tttttcaatt atctggtgaa tccgttcctg ctctacatca taggatatat
     1921 cttctttgaa aagattttgc tcggtctggg ttggatcacc ccggaagccg ccagttacct
     1981 gtggacagga ctcatccttt tgggtgttgc accgtgcatc gcgatggttc ttgtctggac
     2041 tgatcttgcc aaagggaacg gtccactcgg aatcgtgctc atggcatgga actccattat
     2101 tcaagtgctc acaactccat tctacattgc tcttctcgtt ggcacataca tagcgatcga
     2161 catcttcctg atcggccaga gcgtccttct ctatctcggt ctgcccctgc tgtttggtgt
     2221 catcactcgc agggaagtca tcaaacggaa gggggtggaa tggctgaaca ccagactgat
     2281 accgcgcttc aacgctctgc agcttcttgc attactgttt accatggtcg tcatgttctc
     2341 cttgaagggc ggggtcatca ttgacaatcc gaacctcatc tggcagatgg caattccggt
     2401 agtcctgttc ttcttcctgt tgttccacat cgtctattat acaacccgac gcatgggtta
     2461 caactacgag gattcagcta cgatgggatt ccattgcacg ggcaggaact ttgagctggc
     2521 tattgcaatt gcactgacag catttgcatc catgccgatg gttgctgtct caacggttgt
     2581 cgggcccctc atcgagatac ctgtcatgct gggaatagtg tggctgtccc tgaaaaggag
     2641 agatcgcatt atgcagcagg gaaaggcata tttgtcctga tttgtgaggg aactgtgaaa
     2701 aattgtatca tataaagatc agcagcattg gatatcccac ggtctcagtg tcgattacca
     2761 gaagtccgct tcgctctcgt tcaccgctgc atccaacaac ttcgagcttg cgatcgctgt
     2821 tgctatcgcc gttattggca tcgcttcacc agtcgcattc gccaccgtga tcgggcctct
     2881 tgtcgaagtg ccggtactta tatcgcccgt gcatgttccc ttctggctca ggaagagagg
     2941 gtatggcata gacagcgccg attcgtgcaa cctggtgcgg cagtgaatga catgatattc
     3001 attaacaatc cttacaagtt aatatttcaa tttttttaac atgacctttc ctttatgtac
     3061 catgaccata aactaaccat ttggtaatta tcatggatgg atatgcagga aatatagtct
     3121 atgccgatct ttcggcaggg acggttacaa tcaggcccac accaccggaa cttaaaaaaa
     3181 tgtatattgg cgggcgggga tttggtatcc ggctgctgac tgatatggtt gatccgaaga
     3241 tcgacccgct gggagaaaag aatgttgtcg tgtatgcagc cgggccgttg accggtaccg
     3301 gaatcccgtt agggagccgg tacgaggtct cgaccatctc accactcacc gggaccgcga
     3361 tgagcgctaa cagcggtggg gtctttggct ggaaaatgaa gaaggcagga tttgatggag
     3421 tcgttgtttc cggtaaagcg aaaaaaccgg tgtacctcct gctggacaac gggaaggcag
     3481 aaatccgcga tgcagcacat ctctggggta aaaccacggg tcagacaacc gatgccatta
     3541 ctgacgaact gaaggatacg agcgtccgtg tatcctgtat aggaccagcc ggggagaaac
     3601 ttgtgctgtt tgcctgtatc atcaatgaaa agacacgggc tgccgggcgc gggggtgtcg
     3661 gtgctgtgat gggttcaaag aacttaaaag ccattgctgc acggggagat ctgcccatca
     3721 aacctgcgga tgaagagaaa ctcaacatag tcaaggaccg tgtacgcaag aagatcgagg
     3781 ataacgggat tgaaaaagcc ctccacaatt atggtactgc cgtactcgtc aacatagtca
     3841 acgaaaacta tgttctcccg acacggaact tccagaccgc ccattttgcg gatgcggaga
     3901 tggtctccgg tgaaaccata gcaaaaacga tcctcaagaa acccaagggc tgctatgcct
     3961 gtatcgtaca atgcgggcgt gtccatgaat tcgatggtga aacgggagag ggcccggaat
     4021 acgaaccgga ctgggctttt ggacctgact gcgggatcaa tgatctcaag gcagttgtcc
     4081 gggc
//
LOCUS       DASRLV010000111         6445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_120970,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000111 DASRLV010000000
VERSION     DASRLV010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6445
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120970"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VFC43_04450"
     CDS             complement(<1..275)
                     /locus_tag="VFC43_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30467.1"
                     /translation="MVPDIAEIKSDQEKKPENREVPFASHVKALKERVAQVTEDRKMG
                     ADLLFLNTYMASLAMANASRPEIFAYASNRKEYISAKYITKVDTFVK"
     gene            complement(265..2142)
                     /locus_tag="VFC43_04455"
     CDS             complement(265..2142)
                     /locus_tag="VFC43_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HZK30468.1"
                     /translation="MGTLSVAVNLPFKKEIIDSTVDFYNDIESSPLYKMLPANAKEYV
                     KASPHLLEYLHTFPVNTYGIPLFLSELKKDLRSMESPNIIYPVNDTTFIHILPDPDDV
                     RNFYIPIEPSFLHSVNQILPEIETKLINLIDGLEDDPISDKERADVIKKMLASVTVIL
                     PKGMSHAQLQGSASNQQDLKTKFTTFLNTDFTAQKKMKDTKVKHQVPLTPEGKVILTD
                     EEYRAIEYLLIRDKIDMGPLKPFLSDPYIEDISCDGVGPIFIEHKIFKGLKSVIEFKV
                     SSELDEFVVKIAERIKRPITYRSPIVDATLPDGSRINIVYGTAISRHGSNFSIRKVNE
                     VPLSILNIIESNGLDYLAAAYIWICVEYGMSLFVSGETASGKTTLLNAITGFIPPENK
                     IVTIEDTPELNVPHRNWIREVALAKGKGEGTGGSAAGGEVTMFDLLKAALGQRPNQIL
                     VGEIRGVEGAVAFGAMQTGHPVMSTFHAASVEKLIQRLCGDPINIPKTYVDNLNLVII
                     QSAVKRPDGQLVRRMISCNELVGFNAETQGFTFVEMFSWEPITDTFIWSGKGSSYLLE
                     NKIATMLGMPDHKKSEIYLEVEKRAKILERLHKAGYTKFWDLFAMMTKIKKQGLLKIG
                     S"
     gene            complement(2144..2875)
                     /locus_tag="VFC43_04460"
     CDS             complement(2144..2875)
                     /locus_tag="VFC43_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HZK30469.1"
                     /translation="MANISDLMGGEDRRIISTGNTELDKKIADGLPLESLTLIEGEND
                     TGKSVLTQQIIWGAMRQGLSVDLFTTENTSKSFLKQMESMSLDISDYFSYGYIKVFPL
                     HVVGFEWKKEEMAGILARLIKHMENSKSQVIIVDSLTLFTEYAQTDHILTFFTNCKSL
                     VDHGKTILVTLHTYAFEEDTLVRIRSICDAHLNMKKALVGSKYVMVMEVIKVRGAHKT
                     TGNVVSFEVHPGYGMKVIPMSFAKV"
     gene            complement(2894..3388)
                     /locus_tag="VFC43_04465"
     CDS             complement(2894..3388)
                     /locus_tag="VFC43_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30470.1"
                     /translation="MAVAEIIGAAVGVLLLIIVAYVVVGSTLTAAETVSYAQKDLTQL
                     NEARLKTSIALNRNEANLSGQGLNFSVVNNGDEIIRDFSHMDILLSTGEESGGYQHMT
                     YDSITCGKPGTWCIHGNLVPDTIHPGELDPGEKMWIWATFNAGSPVWFQVSTGNGVYA
                     TAYI"
     gene            complement(3395..3898)
                     /locus_tag="VFC43_04470"
     CDS             complement(3395..3898)
                     /locus_tag="VFC43_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HZK30471.1"
                     /translation="MSADSITTALFLITAVVASAVLINAVYPVISNMAGTFSSTTHES
                     DVRIRTDFKIIASYANSTGFAQIWMKNIGSQPIGLGDIQRADLFCGGVGNFNHLAHTS
                     GSLGNNQWTEDFSVPEYDQNNNQFWDPGETLKVTARTTIPSQGGKVTAQFALSNGIWR
                     SIEFTAS"
     gene            complement(3902..4822)
                     /locus_tag="VFC43_04475"
     CDS             complement(3902..4822)
                     /locus_tag="VFC43_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30472.1"
                     /translation="MGVNQSQVDHIITAASEDDPEVKLQNLEREVDLIKTSIKRLLMD
                     IRERMNELENPFTLVTNAPAGSPSGISAEVLEVQEAKNSALEAREAELNARESRMDSA
                     KARDETENKKESPVRKTEPVIPFVDDTKRLLDEQMLAVLRSQIVPQKPMGLSQPVTEQ
                     AGKKLQLQKVYKLFKWTQQAVKKVGHDRLEIILESYRAMGYITKESCDEIREISHLMP
                     ANLGEEHEVGPDEYVSELYELNRILCPDDTSLDRDMIEVMMEQRQSIPAIVSRSSEER
                     IDLPRVAISEKKSSMKKEKDEEWMNLPDRI"
     gene            5241..6251
                     /locus_tag="VFC43_04480"
     CDS             5241..6251
                     /locus_tag="VFC43_04480"
                     /inference="COORDINATES: protein motif:HMM:NF037930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30473.1"
                     /translation="MDDYQTSNLRLAKELSYVILSDLEINQLPISQILMKAKRLARML
                     HDSDAQKWLDYEIMGYPVVFDPGELGSCKKYYTSERPVIRDEKTHRPHSIGLPHLESY
                     INSTKIESVTKIGANESQKMQILNKHHELVNDFERLKTTIHNYITEVNISLSVGDFAE
                     DIFEDTRLAADRFIRENCPRAGEQLLAINERMKYYDPESFSQALHAVQKILVIVADTV
                     LPVGEKIYLDKKGHERSIESHQYLNRIFSYIEQNSRNDPVLFMIESEMAYIFTKSDHA
                     PEKTKGLHERIAKEDVELAIIHMYLIIAEIAKIKKVETIYLKTLPSENYTPNSKNSPN
                     LE"
BASE COUNT         1700 a         1467 c         1381 g         1897 t
ORIGIN      
        1 tttttgacaa acgtatcaac tttggtaata tatttcgctg atatgtactc cttccggttt
       61 gaagcatagg caaatatttc cgggcgggag gcattggcca tggcaagcga tgccatgtag
      121 gtgttcagaa agaggagatc agctcccatt ttccggtctt cggtaacctg tgcaaccctc
      181 tcttttaacg cttttacatg agaggcaaac ggaacttccc ggttttctgg ttttttctcc
      241 tggtcagact ttatttctgc gatatcagga accaatcttc agcaacccct gcttcttgat
      301 cttcgtcatc atggcgaata aatcccagaa cttggtatat cctgccttat ggagccgctc
      361 gagaattttt gcacgctttt ccacttcaag atatatttct gactttttgt gatcgggcat
      421 accgagcatg gtggcaattt tattttcaag taaataggaa ctcccctttc ctgaccagat
      481 aaacgtgtca gtgatcggct cccagctgaa catttcaaca aacgtgaatc cctgggtttc
      541 tgcattgaaa ccaaccagct cgttacagct tatcatccta cgtaccaact ggccgtcggg
      601 acgcttgaca gcgctctgga tgatgaccag attgaggtta tctacgtatg ttttgggaat
      661 attaatgggg tctccacaaa gacgctggat aagtttttcg actgatgcgg cgtggaatgt
      721 gctcatcacc gggtggcctg tctgcatcgc accgaaggca acagctcctt ccacaccccg
      781 tatttcccca acgaggatct ggtttggacg ttgtcccagt gcagccttga gcagatcaaa
      841 catggtcact tctcccccgg cagccgaacc ccccgtcccc tctcctttgc cttttgccag
      901 tgccacttcc cgtatccagt tacggtgagg tacattgagt tctggtgtat cctcaatggt
      961 tacaatcttg ttttcaggag gaataaatcc tgtaattgca ttcaggagtg tggtcttgcc
     1021 gcttgctgtt tcaccggaaa caaagagtga cattccatac tccacgcaaa tccagatata
     1081 cgcggctgcc aggtaatcga gaccattact ttcaataata ttgaggatgc ttaacggtac
     1141 ttcgttcact ttacggatac tgaagttact cccatgccgg ctgatggcag tcccgtaaac
     1201 gatgttgata cgggaaccat ctggcagggt ggcgtcaaca ataggacttc ggtaggtgat
     1261 aggacgtttt atccgttcgg ctatcttcac aacaaattca tccagctcac ttgacacttt
     1321 aaactcaata actgacttga gacccttgaa aattttatgt tcgatgaata tcggaccgac
     1381 accatcacag gatatatctt caatatacgg gtctgacagg aacggtttga gggggcccat
     1441 atcgatctta tcccggatta acaagtactc aatggcacgg tattcttcat cagtaaggat
     1501 aaccttgcct tcgggagtga gggggacctg gtgcttcact ttggtgtcct tcatcttttt
     1561 ctgcgccgtg aaatctgtgt ttaaaaatgt cgtaaacttt gttttaagat cctgctggtt
     1621 ggaggcactt ccctgaagct gtgcgtgact catacctttt ggcagaataa ccgttaccga
     1681 agccagcatt ttctttataa catcagcccg ttccttgtcg gaaatcggat cgtcttcaag
     1741 cccatctatc aggttaatta gtttcgtctc aatttccggg agaatctggt tcacactatg
     1801 gagaaatgag ggttcgatgg ggatgtaaaa gttcctaaca tcatccggat ccggaagaat
     1861 atggataaac gtagtatcat tcaccggata aatgatattg gggctctcca tagagcggag
     1921 gtcttttttt aattccgaga gaaagagagg aataccatag gtgtttaccg ggaaggtatg
     1981 aaggtattca aggaggtgtg ggctggcttt tacatactct ttggcatttg ccgggagcat
     2041 tttatacaac ggacttgatt caatatcatt ataaaaatca accgttgaat caatgatctc
     2101 ctttttgaac ggtagattga ccgctacaga cagagtaccc atatcagacc ttcgcaaaac
     2161 tcattggtat aactttcatc ccataccctg gatgtacttc gaaactgaca acatttccgg
     2221 ttgttttgtg tgcaccacgg accttgatga cctccattac catgacatat ttactgccga
     2281 caagggcttt tttcatgttc aggtgtgcat cacagattga ccgtatcctg accagggtgt
     2341 cttcctcgaa agcatacgta tgaagggtta ctaagatagt ctttccgtga tccaccagtg
     2401 atttgcagtt cgtaaaaaat gtaaggatat ggtcagtttg tgcatattcg gtaaaaagcg
     2461 tcagtgaatc aacgataatt acctgggatt tgctattctc catatgtttg attaaccgtg
     2521 cgagaatgcc ggccatctcc tcttttttcc actcaaagcc gacaacatgg aggggaaaga
     2581 ctttgatgta accataggag aaatagtccg agatgtcaag gctcatggat tccatctgct
     2641 ttaaaaaact cttgcttgtg ttctcagtgg taaagaggtc aaccgacaac ccctgtctca
     2701 ttgcacccca gataatttgc tgcgtaagaa cactcttccc ggtatcattt tccccttcga
     2761 tgagcgtaag agattcaagt ggcagtccat cggcaatttt tttatccagt tcagtattgc
     2821 cggtagaaat tatccggcgg tcttctcccc ccatcagatc agaaatgttt gccatcagga
     2881 cctccttgtt gtattatata tacgctgtag catatacccc attgcctgtc gacacttgaa
     2941 accataccgg gcttcctgcg ttaaaggtcg cccatatcca catcttctcg cccggatcca
     3001 gttccccggg atggattgta tcgggcacca ggttaccatg aatgcaccag gttccgggtt
     3061 tcccacatgt gatactgtca taagtcatat gctggtatcc gccggattcc tcaccagtgc
     3121 tgaggagaat atccatgtga gaaaagtcac gaatgatctc atctccgtta tttaccacgc
     3181 tgaaattcag tccctgcccg gacaggtttg cctcgtttct gttcagggcg atactggttt
     3241 tcaatctcgc ttcgttgagc tgggtcagat ccttttgggc atatgacaca gtttccgctg
     3301 ccgtaagagt actgcccacg actacatatg ctacaataat aaggagaagc acccccaccg
     3361 ctgccccgat aatttcagca acagccatgg gtactcaact cgccgtaaat tctatggatc
     3421 gccagatacc attggaaagt gcaaactggg cggtaacctt gccgccctga gagggaatag
     3481 tcgttctcgc agtgactttc agggtttcgc ccggatccca gaactggtta ttattctgat
     3541 cgtattccgg aacactgaaa tcttcagtcc attggttatt tcctaaactg ccgctggtat
     3601 gtgccagatg attaaaattt ccgacaccgc cacaaaacag atcagccctt tgaatatcgc
     3661 ccaatcctat cggttgtgaa cctatatttt tcatccaaat ctgggcaaaa cctgtcgagt
     3721 tagcatatga ggcaatgatt ttaaaatccg tacggatcct cacatcactt tcatgtgttg
     3781 ttgaagaaaa tgttcctgcc atattggata tgaccggata aactgcatta atcagcactg
     3841 cagacgcgac tactgccgtg atcaaaaata atgcggtagt gattgaatcg gctgacattt
     3901 attaaatcct gtcaggcaaa ttcatccatt cttcatcttt ttctttcttc atggacgatt
     3961 ttttctcact tatggcaacc cggggaaggt cgattctttc ctcactgctt ctcgacacaa
     4021 tggccggtat actctggcgt tgctccatca tgacttctat catgtctcgg tcaagtgaag
     4081 tatcgtccgg gcacaggatt ctgttcaact cataaagttc cgatacatac tcatccgggc
     4141 caacttcatg ttcctcaccc aggtttgcag gcataaggtg ggagatctcg cggatctcat
     4201 cacaggattc ttttgttata tatcccattg ccctgtaaga ttcgaggatg atctcgagac
     4261 gatcatgccc gacctttttg accgcctgct gggtccactt gaagagtttg tagactttct
     4321 ggagctggag ttttttgcct gcctgttctg ttactggctg ggacagaccc atcggttttt
     4381 gaggtactat ctgggacctc aggacagcaa gcatctgttc atcgaggagt ctcttagtat
     4441 catccacaaa tggtatcact ggttcggttt tgcggacagg agattccttt ttattttctg
     4501 tctcatcacg ggctttggcc gaatccattc gggattcacg tgcattcagc tcggcttccc
     4561 tggcttccag tgctgaattt ttggcctcct gtacttcaag tacttctgct gaaatacctg
     4621 acggtgaacc cgccggtgcg ttggttacta aagtaaatgg attttccagt tcattcatcc
     4681 gctcacgaat atccatcagg agacgcttga ttgaggtttt gatgaggtca acctcccgct
     4741 ccagattctg gagcttgact tccggatcat cttcggatgc ggcagtgatg atatggtcaa
     4801 cctgagattg atttaccccc atagtagatc tagtgttata ttttattcaa tactattaaa
     4861 gattttgtta ggaaaatagc aataattgct ggttatagca gaaaaacaaa aaaatttacc
     4921 tgtcaatgtg ggacattggg ttcaaactct gtatttactt ttattgatgt tctgtcacca
     4981 ggatccctgt atcaatactg aaacctgatt tttgtctgtg tatcggtttt ctttttttga
     5041 ctgattataa ccttttccat catcctgcag aatcattgat aatgaataat aaagagcccc
     5101 ggactcattt tttctcaaaa caacatccat cttttatttt aaaaaacggt tcatacaacc
     5161 atagagttat agtccttaat gatgagtatc tattattata caatcagaat tcttgcaggg
     5221 agtagaacaa gcaaatggtt atggatgatt atcagacttc gaacctgaga ctggcaaaag
     5281 aattatccta tgttattctg agtgatctgg aaatcaacca gctgcccata tctcagattt
     5341 taatgaaggc aaaacgcctt gcacgcatgt tacatgattc cgatgcacag aaatggctgg
     5401 attatgagat aatgggttac cccgttgtat ttgatccggg tgaattggga tcctgcaaaa
     5461 aatattatac cagtgaacgc cctgtcatca gggacgaaaa gacccaccgt ccccattcta
     5521 tcggtctgcc ccatctggaa tcatatatca attcgacaaa aattgaatcc gtaacaaaaa
     5581 tcggtgccaa tgaaagccag aagatgcaga tcctgaataa acatcatgag ctggtcaatg
     5641 attttgaacg tctgaaaacc actattcata attacatcac ggaagtaaat atttcacttt
     5701 cagttggaga ctttgccgag gatatttttg aagatacgcg cctcgctgca gacaggttta
     5761 tccgtgaaaa ttgtccgcgg gcgggtgagc agctccttgc cataaacgag aggatgaaat
     5821 attatgatcc ggagtctttt tcacaggcac tccatgcagt gcagaaaatc ctggtaatag
     5881 tagccgatac tgttcttcct gtgggagaaa aaatatatct cgataaaaaa gggcacgaaa
     5941 ggagtataga gtcacaccag taccttaacc ggattttttc ctatattgag cagaattcaa
     6001 gaaatgatcc ggttttattt atgatcgagt cagaaatggc ctatatattt acgaaatctg
     6061 accatgctcc ggaaaaaacc aaaggactgc atgagcggat cgccaaagag gatgtcgagc
     6121 tggcaataat ccatatgtat ctgattatag ctgaaattgc aaaaatcaaa aaagttgaaa
     6181 cgatttatct caagaccctc ccgtcagaga attatactcc gaattcaaag aattctccca
     6241 acctggaatg atattaatat gtgattcaat gaaccttctc atgcaggaac ggaaaaattc
     6301 gaaataaaat aggtatttaa gataatatat tcgaagtggt aatcgttgca ggggttgact
     6361 ttgagaaggg taatgccgca ccgacagtag gtcggatttc gatatttacc cgggtattta
     6421 cggaaatcgg tgtcgtgtta aacgc
//
LOCUS       DASRLV010000112        13460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_276529,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000112 DASRLV010000000
VERSION     DASRLV010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 13460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13460
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276529"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            584..1396
                     /locus_tag="VFC43_04485"
     CDS             584..1396
                     /locus_tag="VFC43_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30474.1"
                     /translation="MVFIIFSGCIWQQKNNTPIPTILVQQSTTGTSFQPSNDRYWIDI
                     DPISDFSTDAAFNIIGSTQLKVNGTTNFPVGTTLHFAIFEENGEREVIKTNIEVLGNS
                     SGPNSFTVVYNMEGNPPGRFLVMLADSTNLYSATSRFNITSALPFPRWIHMDPLENTQ
                     KGKNIFISGTTNLTAGTDITVSYSMLAHSCTPSNIPDKLGERTFCGGSCRPGEGSSHT
                     VRVVEGAGGVNSWNATKNTTGWCTEIYSIGAFAGNGTNATDAGQLIRYCLDQ"
     gene            complement(1418..1885)
                     /gene="moaC"
                     /locus_tag="VFC43_04490"
     CDS             complement(1418..1885)
                     /gene="moaC"
                     /locus_tag="VFC43_04490"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HZK30475.1"
                     /translation="MAEFTHIRNDRAQMVDISAKDEVTREAVAVGKIFLRPETMQAIR
                     EGTVVKGNVLSTARVAATLSVKNTPSLIPMCHSIPISAITVDFFEGDGFIESTVRVKS
                     TGKTGVEMEALVGVSVALLTIWDMVKSAEKDADGQYPVTRIEGIRVLEKKKGE"
     gene            complement(1886..3286)
                     /locus_tag="VFC43_04495"
     CDS             complement(1886..3286)
                     /locus_tag="VFC43_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HZK30476.1"
                     /translation="MRPELIDGFLETGIISPARMRVVDINATALGVSDLQLMESAGRA
                     LADRALAMKPDQVLVLCGKGNNGGDGMVAARHLQRGVTTDVCYLDSGKRSGSCEHQLA
                     ALKHCRVVLHPFQCRDDLVHNRSLFEHADVIVDALLGIGATGSLDEPLSTCIAMANAS
                     RAKIIAADIPTPGMRVDRICAFHRAKTEGSEVIDIGIPVEAECCVGPGDLTMLPSRRK
                     KSHKGIGGEVLVIGGGPCQGAPYLAGLGALRAGADIVRIASPVFEPIPDLIYERLEGE
                     RIGEEHLERLMVLAARADAVICGNGLGPDSHPVMCAIAPCCKKAVIDADALRLPLPVA
                     QEETLYTPHAGEFMRITSTRLSDDTLERAVAARGAQLPGTVLLKGSIDIITDGNRVRF
                     NRTGDPAMTVGGTGDVLAGIAGALLCHLPAFEAGCIAAYVNGMAGQFVAAKRGEGMLA
                     SDLVDRIPAELYTKGG"
     gene            3331..4731
                     /locus_tag="VFC43_04500"
     CDS             3331..4731
                     /locus_tag="VFC43_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HZK30477.1"
                     /translation="MGFNDDVKAAAEQIKAAPEITIISHIDADGIACEAILSQAISRL
                     GIPVKSVFVRQLEPLTMPQVPSDSSLKIFSDLGAGQQNLLFERGLTEKEVVIIDHHVS
                     QPCERQYTQVNCLPYGHSRMSAAGVCYFVAKELDEVNIDLAKLAIVGNVGDMMAREKC
                     GLVGPARDIIVEDGMRHQSVEVRKKDLNCYGTATRPVHLSLAYNDDPFIRGISNNPEG
                     ARQFLKRLGIRQQNDNGRWFVWEEMSAEDKRTIISALAEQLIANGERVDRLLAETYGF
                     PGELPRTPLRNAQEYATMLNACGRWSKPHVGGAILRGDRGIAYRDAEHMLNNHRAIIR
                     NLLQYIIDTGVKELENLQWIHVGGRYPDTIVGIGAGMALSKLNSKKPILVMCEVPEDT
                     SLTKISMRTNERVVERGVDLQQALSDASSEYGGGGGGHKIAAGAYIPKSAEQEFLDRV
                     NRILGEQFAAASADNR"
     gene            4676..5230
                     /locus_tag="VFC43_04505"
     CDS             4676..5230
                     /locus_tag="VFC43_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PUA domain-containing protein"
                     /protein_id="HZK30478.1"
                     /translation="MSTGYSENSSLRRVQTIADYQFGCRAGTGLFPEGCTFIFSTTGR
                     IRQILFDGIRLATVRASDGRLTLGIEGAKRLKSLLPVPGYRVVIRDDVAMFVAQGKNA
                     FSKHVTAADPVIRAGDDVLVVASDDRLIACGAAVISGREMLAFNYGVAVRVRQGSEKD
                     ASGKYQPTPDEGDDEEARHAGRAD"
     gene            5154..5495
                     /locus_tag="VFC43_04510"
     CDS             5154..5495
                     /locus_tag="VFC43_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nascent polypeptide-associated complex protein"
                     /protein_id="HZK30479.1"
                     /translation="MLPGNINPRQMKAMMKKLGMQVEQIEDVQSIVIKTPKGNYIFDS
                     AEVSAMTMQGTTTYQVIGDIRFEPAAPEILKEDIAMVAEQAKVSEEKAKEALMATKGD
                     IAEAIMRLSSS"
     gene            5492..6217
                     /locus_tag="VFC43_04515"
     CDS             5492..6217
                     /locus_tag="VFC43_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate
                     carboxylmethyltransferase"
                     /protein_id="HZK30480.1"
                     /translation="MIEQGDRVLLVGQGREFFVKAGPGTLGTDKGQLDLSTIVGKAGG
                     DTLTTHNGSQFIIRVPRPTDFFTYGKRSGAPMLPKDIGLVIAYTGMNHNDDVLDAGTG
                     SGIAAIYFGGVAKTVRTYEVRPEFSALAMKNITEAKLDNVEAVSKDFLESEGTYDVVH
                     LDLQIQAEHMTHAFSLLKTGGYLACYTPFLEQMAIVVDAATPLFCEVHTHELIEREMT
                     RSKRGTRPSTSVCHSGYITIARK"
     gene            complement(6474..7586)
                     /locus_tag="VFC43_04520"
     CDS             complement(6474..7586)
                     /locus_tag="VFC43_04520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZK30481.1"
                     /translation="MLLLFASILFFIAFVPYLIYLAGIWFGKKSPQIQAPSSYPDISI
                     IISAYNEELSIVKRLDNLQESRYPLEQIEIIVVDDCSSDNTKNLAKSWLGRSGIRYQF
                     IENNERLGTNRSYNLAISRAHNPVIITTDADVFFDPDALRYLIGRLVSDKKIAAVTGE
                     LRPIANANSTTLLEGVYRSFYGRMCEWESALDSTYNFNGALVAFRSGLITRINDKLGA
                     DDANTAFEAIRRGYRAVYEKKAIVYEDIPKSFAIQYRQKARRATGLIEATLSNLDLLG
                     KNTPFSRFFYPIRIFMYVCSPLIFSLSVLLFCSWLFLTSPLLLLCVLVIFIGAGIIWR
                     QNLVFAFILNQFYLMAGLANLGKDMRVWESTSAKKQ"
     gene            complement(7594..9024)
                     /locus_tag="VFC43_04525"
     CDS             complement(7594..9024)
                     /locus_tag="VFC43_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HZK30482.1"
                     /translation="MALEALIKKRGPIKKIGVLGMGYVGIPAAALFADSPVFDVVYGF
                     QRDSASSGYKIAMLNRGESPLKGEEPGLEDLLKKVVAAGKFSCTSDFSKIAGLDAVTL
                     SIQTPFLNKEDLLPDFSALKEGIGQVGRYLTPGTLVVLESTVTPGTTNTLAKEILEQE
                     SGLRAGTDFALAHAPERVMVGRLLRNIREHDRIVGGIDPLSTKRATELYAPVLTLGKV
                     IPMSATAAEVTKTTENTFRDLQIAAMNELALYCEAMGINVYDVRTGVDSLKGEGITRA
                     MLWPGAGVGGHCLTKDTYHLERGVQMLGKDLLDYPKGEPSLYVLARRINDFMPSHMFR
                     LTTDALQRAGCLLKGSNVAILGWAFLANSDDTRNTPAEPYRDDLIRAGASVRVHDPYV
                     MEYPGVPVLHDLDESIRGADAIVIFAGHHQYKNLVPGHIRALSGKPHPVIIDGRNLID
                     ADLFIRHGFVYKGIGRGDKNMHAVSG"
     gene            complement(9046..9951)
                     /locus_tag="VFC43_04530"
     CDS             complement(9046..9951)
                     /locus_tag="VFC43_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZK30483.1"
                     /translation="MDIGVIGVGAMGKNHVRVYSELKRVHSLGIYDVNTQAAKMLGEK
                     HGATVYESISELLDNSDAVSVVVPTQFHSGVVGQVFAKKKSVLIEKPICATVKEAKKL
                     MKTAPEGITVGVGHIERFNPIVAEIKKIVKTPLYMEFKRHNPASARITGSSVVEDLMI
                     HDIDILLNVFFPDPCDIHSIGNADVMSVLMNCDGIPVSLSASRKASKKIRLFYVEDEE
                     FTIEGNFMTQEVTIHRKPGQYHVEAERYVQENIIEKVMVNTVEPLKRELETFIDCVDK
                     GQPFPIAPSQAIRNLEFCEKVSSAI"
     gene            complement(9952..11043)
                     /locus_tag="VFC43_04535"
     CDS             complement(9952..11043)
                     /locus_tag="VFC43_04535"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZK30484.1"
                     /translation="MQIPVARPAIGQDEISAVTAVLESGMLAAGERVAEFEKKFADYC
                     GATHAVAMNNGTAALHAALLAADIGPGDEVIVPSFTFFATASSVSMTGAKPVFVDVNE
                     KTFCIDPAQVENHITPKTKAVIGVHLFGQPFDVQAIKQVCESHNLTLIEDAAQAQGAM
                     WNSEKAGSMGHMGCFSFYATKNMTTGEGGMVTTSDKALNDRLRLLINHGQSEKYLHTR
                     IGYNYRLTDVAAAIGLVQLKKLEKFNTRRRKNAEYFDAHISVKGILTPFVAPNMHHVY
                     HQYVLRLTDEFGMKRADFIEYLKAKGIGTAIHYPIPLHHQPVYARANEPDSCPVSTSL
                     ASSVLSIPVHPLLDQKELSYICEVINRVK"
     gene            11231..12133
                     /gene="pyrB"
                     /locus_tag="VFC43_04540"
     CDS             11231..12133
                     /gene="pyrB"
                     /locus_tag="VFC43_04540"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HZK30485.1"
                     /translation="METEHLISIGDFDRDGIDTMLDHARQVDDGLYDKHALQGKILAT
                     LFFEPSTRTRMSFEAAMARLGGTSLSVGSVEACSIAKGETLADTIRVVSGYADAIVIR
                     HPKEGAARLASEFATVPVINAGDGAGQHPSQTLLDLYTIRQSMPIDNIDVALVGDLRY
                     GRTAHSLASALSLYNARLHTISPKGLELPATLAAGLSDKGMEIIEHDDLRDVICDIDV
                     LYVTRIQRERFPDSASYFNVASNYRITPELLTGAKNHLIILHPLPRVDEIDPRVDELP
                     YARYFEQSRNGVPIRMAMLMRLMK"
     gene            12130..12603
                     /gene="pyrI"
                     /locus_tag="VFC43_04545"
     CDS             12130..12603
                     /gene="pyrI"
                     /locus_tag="VFC43_04545"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HZK30486.1"
                     /translation="MKKTEAEENERLLVRRIKDGTVIDHIQGGEALNVVKILGITGTT
                     HETLSIATNVPSHDMKCKDIVKLTNRELSKEEVDRIALISPRATINIIRNFKVCEKKG
                     VEIPTLIEGIVRCPNPGCISNTNEPIQSKFKVLPKGLLCHYCDWVITKDLTSHII"
     gene            12771..>13460
                     /locus_tag="VFC43_04550"
     CDS             12771..>13460
                     /locus_tag="VFC43_04550"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HZK30487.1"
                     /translation="MITKRGNYALYAAIFVLLLALVLPGACASSTEQPGYITVGLAPV
                     ADFDALYAYNTVPATVAFRDHSTGTTPLNYAWEFGDGATSTEQNPTHTFVRKGLYTVK
                     LTVTNVYGSSTEAKENYIAIGLAPKADFSGSPTTGNTPLTVEFTDRSTGYPTTWKWNF
                     GDGKESSDPNPVHTFWSSGDYTVTLTASNEYGTSDVTKAYLIHVIPALKAKFIADPKQ
                     GKAPLVVKFSDL"
BASE COUNT         3390 a         3447 c         3347 g         3276 t
ORIGIN      
        1 cgcttttttc cggtactttc catgcctccc tgacgaaggc gtacattgat tttaccatga
       61 taatcaccta aggttcggct catttttgag ctacattcct ttcaacggga cgcatcccgt
      121 attcccacat actttaaccg gggaactatt aaagtactgg tcgggggctg caggagaacc
      181 tgtttgtctg aatgatctca aatttctcgt aatgaggaac gttaaaaaat ttcatacgtg
      241 tgactgctgc cgcaccaaaa agggacgccc ccgcagcggc ctaaatatcg ttgaaattcc
      301 ccacaggtaa tgagtagcgc aaaacccgac gaggcgtttt tgcgcgttgt gtaatcgtaa
      361 cgggggatgt ctcaatggcg caggtcagct tccgagagcg ttatgaagcc ttgtaccagt
      421 tggtcatttg ccgtggaggt acagaattcc cttctgactc gaaccattaa gattacagat
      481 acaatattag tggatgaata atcaataaat cgtttgtttg gttatcagag cggattcaat
      541 gcaccatgtc aatttaagat ctatcataat cgcagggttc atcttggtat tcatcatctt
      601 ttccggatgt atttggcaac aaaaaaataa cacacccatt ccaaccattc ttgttcagca
      661 atcaacaaca gggacttctt tccagccttc taatgacagg tattggatcg atatcgatcc
      721 catcagcgac tttagcactg atgcagcatt caacattatc ggatcaacac aactcaaggt
      781 caatggcaca acgaattttc ctgtgggaac cacgttgcat ttcgctatct ttgaagaaaa
      841 cggagaacgg gaagtaataa aaacaaatat tgaagtccta ggtaatagtt cgggaccaaa
      901 ttcattcact gttgtatata atatggaagg caatcccccc ggacgctttc tggtgatgct
      961 tgcagattca actaatctat attcagcaac atcgcgtttc aatattactt cagctctgcc
     1021 cttccccaga tggatccata tggatcctct tgaaaacaca cagaaaggta agaatatttt
     1081 tatttccggt actacgaact tgaccgccgg gaccgatata acggtgagtt attctatgct
     1141 cgcccatagc tgcacaccat ctaatattcc ggataaattg ggggagagaa ctttctgcgg
     1201 cgggagttgc cgcccaggag aaggatccag tcatacggtc cgggtcgttg aaggcgcagg
     1261 aggggtaaat tcttggaatg cgaccaaaaa tacaactggt tggtgcacag aaatttacag
     1321 tatcggagcc tttgcaggaa atggaacaaa tgcaacagat gctggccaat taattcggta
     1381 ttgtctcgat caataaatta tgcttaaaag ggagaattca ctcccccttc ttcttctcca
     1441 gcacccttat gccctctatc ctcgttaccg ggtactgccc gtcggcatcc ttttctgcag
     1501 attttaccat gtcccagatg gtgagcagcg caacggaaac cccgaccagc gcctccatct
     1561 ccacgccggt ctttccggtg gatttcacac ggaccgttga ctcaatgaat ccgtcacctt
     1621 caaaaaaatc aacggtaatc gcactgatag ggattgaatg gcacatgggt atcagggagg
     1681 gagtgttttt gacggaaagt gtcgctgcaa cacgggcagt ggaaagcaca tttcctttca
     1741 cgaccgtgcc ctcccgtatc gcctgcatgg tctcaggtcg caagaagatt tttcccacgg
     1801 caactgcttc acgggtcacc tcatccttgg cactgatatc caccatctgc gcgcggtcat
     1861 tccggatatg cgtaaactcc gccattcacc caccttttgt atataattct gcagggatcc
     1921 tgtccaccag atcagaagcc agcataccct caccgcgctt tgcggcaaca aactggcctg
     1981 ccatgccgtt cacgtacgct gcaatgcatc ccgcctcaaa tgcaggcagg tgacagagca
     2041 gggccccggc gatcccggca agcacatccc cggtaccgcc cacggtcatc gccgggtccc
     2101 cggtacggtt gaaccgcacc cggttcccgt cagttatgat atctatggat cctttgagga
     2161 gcaccgtgcc cggcagctgt gcacctctcg cagccactgc acgctcaaga gtatcgtccg
     2221 aaagcctcgt tgatgttatc cgcataaact ctcccgcatg cggtgtataa agggtctctt
     2281 cctgtgcaac cggaaggggt aaccgcaggg catccgcatc aatcaccgct tttttacagc
     2341 aaggtgcaat cgcacacatt actggatggc tgtcgggacc caggccgttt ccgcagatga
     2401 ccgcatcagc acgtgccgca aggaccatca gccgttcaag gtgttcttct cctatccgct
     2461 ctccttccag gcgttcatag atcagatcgg gaatgggctc aaagaccgga gaagcgattc
     2521 tgacgatatc tgcacctgcc cgcagtgctc ccagtcctgc caggtacggt gcaccctggc
     2581 agggtccgcc accgataacc aacacttccc cccctatgcc cttatgagac tttttacgcc
     2641 gtgaaggaag catagttaag tcccccgggc cgacacaaca ttccgcttca accggtatcc
     2701 cgatatcaat gacctcggat ccttcagtct tggcccggtg gaatgcacat atccggtcta
     2761 cccgcatgcc cggcgtgggt atatctgctg caataatctt tgcccgggat gcatttgcca
     2821 tcgcgataca ggttgagagg ggctcatcta aagaaccggt tgcaccaatt cccagtaatg
     2881 catcgacaat cacatctgca tgctcaaaca gtgatctgtt atgtaccagg tcgtcgcggc
     2941 actggaacgg gtgaagaaca acccggcagt gtttcaatgc cgccagctgg tgctcgcagg
     3001 aaccactcct ttttcccgaa tcgaggtagc agacatcggt agttactccc cgctggagat
     3061 gacgtgcggc caccatcccg tcaccaccgt tattgccttt cccgcacagt acaaggacct
     3121 ggtcaggttt catggcaagc gcccggtcag cgagtgcccg ccccgcactt tccatcagct
     3181 ggagatcgga aacacccagt gcggtcgcat ttatatcaac cacacgcatc ctggcaggac
     3241 tgattattcc tgtctccaga aatccgtcaa tcagttccgg tcgcattgtt gagcacactt
     3301 catctgtatg aactccataa ataatatgca atgggtttta acgatgatgt gaaagcagcc
     3361 gcagaacaga ttaaggcagc accggaaata accatcatct cccatattga tgcggatggg
     3421 atcgcctgtg aggctatcct ttcacaggca atctcccggt tggggattcc ggtgaagtcc
     3481 gtttttgtcc gacaactcga acccttaacc atgccccagg taccgtccga ctcatcgctc
     3541 aaaatttttt ccgatctagg agcaggacag cagaatcttc tttttgagcg tggtttaacc
     3601 gaaaaagaag tcgtcatcat cgaccaccac gtgagccagc cctgtgaacg tcagtatacc
     3661 caggtaaact gcctgcccta tggccactcg agaatgagtg ccgccggggt ctgttacttc
     3721 gtagcaaaag aactcgacga ggtaaacatt gatctcgcca aactcgccat cgtcggcaat
     3781 gtcggggaca tgatggcacg ggaaaaatgc gggcttgttg gtcccgcccg cgatattatt
     3841 gtggaggacg gtatgcggca ccagtcggtc gaagtgcgaa aaaaggatct caactgctat
     3901 ggcaccgcaa cccgcccggt ccacctctcg cttgcgtata atgatgaccc gtttatccgc
     3961 gggatcagca ataatcctga aggggcacgg cagttcttaa aacgcctggg aatccgccag
     4021 cagaatgata acgggcgctg gtttgtatgg gaagagatgt ccgctgagga taaaaggacc
     4081 ataatttccg cgcttgccga gcagttgatc gcaaacgggg aacgggttga ccggcttttg
     4141 gcagaaacct acgggtttcc cggtgagctc ccccgcaccc cgctgcggaa tgcacaggaa
     4201 tatgctacca tgctgaatgc ctgcgggcgc tggtccaagc cccatgtcgg cggcgcaatc
     4261 ctccgggggg accggggaat tgcctaccgt gacgccgaac atatgctcaa caaccaccgg
     4321 gcaattatcc gcaacctgct ccagtatatc attgataccg gcgtgaaaga attagagaac
     4381 ctgcagtgga tccatgtagg agggcggtac ccggatacga ttgtcggaat tggtgcagga
     4441 atggcactct caaaactcaa cagcaaaaaa cccatcctgg tcatgtgtga agtgcctgaa
     4501 gataccagcc tgacaaaaat atccatgagg acaaatgaac gcgtagtgga acggggagtc
     4561 gacctccagc aggcactcag tgatgcctct tctgaatatg gcggcggggg cggcggacat
     4621 aagattgcag cgggtgcata tatcccgaaa tcggcagagc aggagttttt ggatcgtgtc
     4681 aacaggatac tcggagaaca gttcgctgcg gcgagtgcag acaatcgctg attaccagtt
     4741 cgggtgccgg gccggcaccg ggctttttcc cgaaggctgc accttcatct tctcgacaac
     4801 cgggaggatc cggcaaatcc tgtttgacgg tatacgtctg gcgaccgtgc gggcttcgga
     4861 tggcaggctg acgctcggga tcgaaggggc aaagcgcttg aagagtctgc ttcccgttcc
     4921 cggttaccgg gtggttatcc gtgatgatgt ggcaatgttt gtagctcagg gaaagaatgc
     4981 cttttcaaaa cacgtcaccg ctgctgatcc tgtaatccgc gcgggagatg atgtgctggt
     5041 cgttgccagc gatgaccgcc ttattgcatg cggtgctgcg gtaatttcgg gcagggagat
     5101 gcttgcattt aattatggag tagcagtaag agtacggcag ggtagtgaga aagatgcttc
     5161 cgggaaatat caacccacgc cagatgaagg cgatgatgaa gaagctcggc atgcaggtcg
     5221 agcagattga agacgtgcag agtatagtca tcaagacacc gaagggcaat tacatctttg
     5281 attccgccga ggtttcggca atgaccatgc aggggacgac gacctaccag gtcatcggcg
     5341 atatccggtt cgaaccggca gcaccggaaa ttctcaaaga ggacattgcg atggtggctg
     5401 aacaggcaaa agtctctgaa gagaaggcaa aagaggcact tatggcaaca aaaggcgata
     5461 tcgcagaagc aatcatgcgc ctctcctctt catgatagaa cagggagacc gtgtactcct
     5521 cgtgggccaa ggaagagaat ttttcgtaaa agcggggccg gggacactcg gtaccgataa
     5581 aggacagctc gacctttcaa ccattgtcgg gaaagcgggc ggggatacgc tcacgaccca
     5641 caacggctcg cagtttatca tccgcgtccc gcgtccaacg gactttttta cctacggcaa
     5701 gcgttcgggt gcaccgatgc tgccaaagga tatcggcctc gtcatcgctt ataccgggat
     5761 gaaccacaac gatgatgtac ttgatgccgg cacgggaagc ggaattgccg ccatctactt
     5821 cggtggcgtg gcaaagacag tgaggacgta cgaggtccgc ccggaatttt ccgcgctcgc
     5881 gatgaagaac atcaccgagg cgaaactgga caatgtggaa gcggtatcaa aagacttcct
     5941 tgaatcagaa ggcacgtatg atgttgtcca cctcgacctc cagatccagg cagaacatat
     6001 gactcacgcg ttttccctgc tgaagaccgg gggataccta gcgtgttata caccattttt
     6061 agagcagatg gcgatcgttg ttgatgctgc cacaccgctc ttttgtgagg tgcacacgca
     6121 cgagctcata gaacgagaaa tgacacggtc aaaacgaggg acaaggcctt ccacaagcgt
     6181 gtgccattct ggttatatca ccattgcaag aaaataattg tcttttttaa aaaacaggca
     6241 cagtccacta tgaaaaatcg caaaaatgat aggagtgacg cttcgctaca agtggatcac
     6301 ctgggcgatg tttcatggtt cccggtctgg agcccgtcct cccatagggg aggctttcac
     6361 aaaaatgtat acatatcagt tcgatcgggc tctctgattg gtgacattaa cacacagtcc
     6421 gttatgtagt attaaaaaaa attcacactg gggaatgcgt taaaaaccaa caactactgt
     6481 tttttcgcag atgtgctctc ccatacccgc atatctttac caagatttgc caggcccgcc
     6541 atgagataaa actggttcag gatgaacgca aaaaccaggt tttgtcgcca gatgatccct
     6601 gccccgataa aaattacaag aacacagaga agcaggagcg gactggtgag gaatagccag
     6661 gagcagaata acaaaaccga gagggaaaaa atgagtggcg agcacacata cataaaaatc
     6721 ctgatcgggt aaaaaaaacg tgagaaaggt gtgtttttcc ctaaaaggtc caggtttgag
     6781 agcgttgctt caatgagtcc ggtggccctc cttgccttct ggcggtactg gatcgcaaaa
     6841 ctttttggga tatcctcata cacgattgcc tttttctcgt acacggcgcg atatcccctc
     6901 cgtattgctt caaaagctgt gtttgcatcg tcagccccga gtttatcgtt aatcctcgtt
     6961 atgaggccgg accggaatgc aaccagtgcc ccgttaaaat tataggtaga atcaagggcg
     7021 ctctcccatt cgcacatgcg cccgtaaaac gagcggtaga ctccttctaa cagggtggtg
     7081 ctgttggcat tggctattgg cctgagttcg ccggttactg cggcgatttt tttatcgctt
     7141 accaggcgtc ctataagata ccgcagggca tcgggatcaa aaaaaacatc agcatcagtg
     7201 gtgataatga ccgggttgtg tgccctgctt atggcaagat tatacgacct gttggttccc
     7261 agccgttcat tgttttctat gaactgatag cggataccgg aacgcccgag ccaggatttt
     7321 gccaggtttt ttgtattgtc actggagcag tcatccacca cgatgatctc gatctgctcc
     7381 aggggataac gtgattcctg taagttgtcc agccgtttca ctatagagag ttcttcatta
     7441 tatgccgaga tgattatgct gatatcaggg tagctggagg gagcctggat ttgtggggat
     7501 tttttaccaa accagatacc ggctaaatat atgaggtagg gaacgaatgc aatgaaaaaa
     7561 aggatggatg caaacaagag cagcattctg aaattaccct gaaacagcat gcatgttttt
     7621 atcgccccgc ccgattccct tgtatacaaa accgtgccgg ataaagaggt cggcgtcgat
     7681 caggttcctt ccatcaatga taaccgggtg tggctttccg gacagggccc gtatatggcc
     7741 cggaaccaga ttcttgtact ggtgatgccc ggcaaagatc actatggcat ctgctccccg
     7801 tatcgattca tccaggtcat ggaggacagg aacgccggga tattccatca catacggatc
     7861 atggacacgc acagacgcac cggctctgat gaggtcatcc cggtagggtt ctgcgggggt
     7921 gttccgcgta tcgtctgaat tggcaaggaa cgcccatccg aggatggcaa catttgaccc
     7981 tttcagtaaa caccctgccc gttggagggc atcggtggtg agccggaaca tatgggatgg
     8041 catgaaatcg ttgatgcggc gtgcaagcac gtagagcgag ggttcacctt ttgggtaatc
     8101 cagaaggtct tttcccagca tctggacgcc ccgttcgaga tgatacgtgt ctttggtcag
     8161 gcagtgtccg ccaacccctg cccctggcca gagcatggcc cgggtgatac cttcaccctt
     8221 gagactgtcc acgccagtac gtacatcata cacattgatc cccattgctt cacagtagag
     8281 cgcaagttca ttcatggcgg cgatctggag gtcccggaac gtgttttctg tggtttttgt
     8341 gacttcggca gcagttgcag acatgggtat cactttaccc agcgtcagga cgggcgcata
     8401 aagttctgtt gccctctttg tgcttaacgg gtcaatgcca ccaacaatcc ggtcatgttc
     8461 acggatattg cgcagcagcc ggcccaccat tacccgttcg ggtgcatggg caagggcaaa
     8521 atcagtaccc gccctgagtc ctgattcctg ttcaaggatc tcttttgcaa gcgtgtttgt
     8581 cgtacctggg gtaacggtgg attcaaggac gaccagcgtt cctggtgtga gataccgtcc
     8641 gacctgcccg attccctctt taagggcaga aaaatccggc aggaggtctt ctttatttaa
     8701 aaaaggagtc tggatagaga gggttaccgc gtccagtcct gcaatctttg aaaagtctga
     8761 ggtacaggaa aactttcctg cggctacgac ttttttcaag aggtcttcaa gtcccggctc
     8821 ttcaccttta agggggcttt caccccggtt cagcatggcg attttatagc ctgatgaggc
     8881 agagtcccgc tggaacccgt acacaacatc aaacaccggg gagtctgcaa agagcgccgc
     8941 tgcaggaata ccaacatacc ccatgccaag aaccccgatt ttttttatcg gcccgcgttt
     9001 ctttatcaat gcttcaagtg ccattgtcag attcacatcc ttatattaaa tagccgaact
     9061 gactttttcg cagaactcaa ggttgcgtat cgcctgcgaa ggagcgatcg ggaacggctg
     9121 gcccttgtcg acacaatcga tgaacgtctc aagttcgcgt tttaaaggct cgacggtatt
     9181 gaccatcact ttctcgatga tattttcctg cacgtaccgt tcggcttcga catggtactg
     9241 tccgggtttg cggtggatgg tgacctcctg agtcatgaaa ttgccttcaa tagtgaactc
     9301 ttcatcttca acatagaaca aacggatttt cttggacgcc ttcctgcttg ctgaaagcga
     9361 aaccgggatt ccatcgcagt tcatgagcac gctcatgaca tctgcattac ctatactgtg
     9421 gatatcacag gggtcgggaa agaatacatt cagaagtata tcaatatcat ggatcatcag
     9481 gtcttcaaca accgaacttc cggtgatccg ggccgatgcc ggattatgcc gtttaaactc
     9541 catgtacagc ggggttttca cgatcttttt tatttcagca acgatagggt tgaaccgttc
     9601 gatatgcccg acaccgacag taattccttc aggagcggtt ttcatcagct tttttgcttc
     9661 ttttaccgtt gcacagatag gtttttcgat aagaacggat tttttcttgg caaagacctg
     9721 cccgaccacg cctgagtgga actgcgtcgg cacaacaaca ctgactgcat cagaattgtc
     9781 aagcagttcg ctgatcgatt cgtagactgt tgcaccgtgt ttttccccga gcatttttgc
     9841 agcctgggta ttcacatcgt aaataccaag ggagtgcaca cgttttaatt cggaataaac
     9901 ccgcacgtgg ttctttccca ttgcaccgac gccgatcact ccgatatcca ttcatttcac
     9961 ccggttgatg acttcgcaga tataggaaag ctctttctgg tctagcaatg ggtgaacagg
    10021 gatactcagc acagaagagg caagactggt cgataccgga caggaatcgg gttcattggc
    10081 cctggcatat accggctggt gatggagagg gattgggtag tgtattgcag tgccgatacc
    10141 ctttgcctta aggtattcaa taaaatcagc acgtttcatc ccgaattcat cggtcaggcg
    10201 gagcacgtac tggtgatata cgtggtgcat attcggagca acaaagggcg tgagaattcc
    10261 tttaactgat atatgagcat cgaaatactc ggcgtttttc cggcggcgcg tgttgaactt
    10321 ctccagcttt ttcagctgta caagaccaat cgccgcagcc acatcggtca ggcggtaatt
    10381 gtacccgatc cgggtatgga ggtatttctc gctctgccca tggttgatga gcaggcgcag
    10441 gcgatcgttc aatgccttgt cactggtagt gaccatgccc ccctcgccag tggtcatgtt
    10501 ttttgtagca tagaatgaga aacagcccat gtgacccatt gagccggcct tttcgctgtt
    10561 ccacatcgca ccctgagcct gtgctgcatc ctcgattaac gtaagattat gtgattcgca
    10621 gacctgtttt attgcctgaa catcgaatgg ctggccaaag agatggacac cgatgaccgc
    10681 ttttgtcttt ggggttatgt ggttttccac ctgagccggg tcgatgcaga atgtcttttc
    10741 gttcacgtca acaaagaccg gctttgcccc ggtcattgat acagacgatg cagtagcaaa
    10801 aaacgtaaag gaggggacga tcacttcatc tcccggaccg atatctgcag ccagcagcgc
    10861 agcatggagt gcggctgtac cgttattcat cgcaacagca tgtgttgccc cgcaataatc
    10921 ggcaaatttt ttctcaaatt ccgcaactcg ttctcctgcg gccagcatgc cggattccag
    10981 cacagcggtt accgcagaga tctcatcctg accaatggca ggtcgcgcaa caggtatctg
    11041 cactactatt caccctatta tcttgtttgt ttcaataaaa atgcgcaatg attggataaa
    11101 tataattgct gcttaaggat agaaggtttg ttcctgtttt ttggtttttc agatgtaact
    11161 gctataaaac cccaaagata ggtaattaga acaggaacat cctatattcg tacactaaaa
    11221 accgggaggg gtggaaacgg aacatctcat atccatcggc gattttgatc gcgacgggat
    11281 cgacaccatg ctggatcatg cgcggcaggt tgatgatggg ctctatgata aacatgccct
    11341 gcagggtaaa atccttgcaa ctcttttctt tgaacccagt accaggaccc ggatgtcgtt
    11401 tgaagctgcc atggcccggt tgggaggaac ctctctttcc gtggggagtg tcgaagcctg
    11461 ctcaattgca aaaggtgaga ccctcgccga tacgatccgg gtggtaagcg ggtatgcgga
    11521 tgcaatcgtg atccggcacc caaaagaggg agcggccagg ctggcctcag aatttgccac
    11581 ggttcccgtc atcaatgcag gagacggtgc gggtcagcac ccctcccaga cgctcctgga
    11641 tttatatacg atccgccagt cgatgccgat cgataatatc gatgttgcac tcgtaggaga
    11701 cctcaggtat ggtcgcactg cacactcact ggcatctgcc ctctcgctct ataatgcacg
    11761 tttgcacaca atttccccaa aaggactgga gcttcctgca acgcttgctg ccgggctgtc
    11821 tgataagggt atggaaatta tcgaacatga cgatctcagg gacgtaatct gtgatattga
    11881 cgtcctctac gtcacccgaa tccagaggga acggttcccg gactcggcgt cttacttcaa
    11941 cgtagcttca aactatcgta ttacacctga gcttctgacc ggtgcaaaaa accatcttat
    12001 catccttcat ccgctcccgc gggttgacga gatagaccca agggttgacg aattgcccta
    12061 tgcccgttac tttgaacaat cacggaatgg tgtcccgata cgtatggcga tgctcatgag
    12121 gctgatgaaa tgaagaaaac cgaagccgaa gaaaatgaaa gactcctcgt ccgccggatc
    12181 aaggacggga ctgtgatcga tcacatccag gggggcgagg cactcaatgt cgttaaaatt
    12241 ctgggaatta cgggcacaac tcatgagacg ctttccatcg caaccaatgt cccgagccat
    12301 gatatgaagt gcaaggatat cgtcaaactt accaaccggg agctttcaaa agaggaagtc
    12361 gaccgaatcg ctctcatttc cccccgtgca acgatcaata tcatacggaa tttcaaggta
    12421 tgtgaaaaga aaggcgttga gatccctacg ctcatcgaag gtatcgtccg ttgccccaat
    12481 ccgggatgta tttcaaatac caatgaacct atccagagca aattcaaggt gctgccaaaa
    12541 ggattgcttt gtcactactg tgactgggtc atcaccaagg acctgaccag tcacataatc
    12601 tgatttttat tcatgcattt cttcatattg aaagtgtgga tccaataatt ccaatatctc
    12661 ccaaataacc ctgatttttt taaaaaatat acaaaataat cgttgtttga gattatccgg
    12721 tttgggagaa acgttaatat atagactctc gtaaatatgt ctggtttata atgataacaa
    12781 aacgaggtaa ttacgcttta tatgcagcga tttttgtgct gctccttgcc ctggttcttc
    12841 ccggggcttg tgcttccagt acagagcaac cgggctacat cacggtaggt ctggcaccag
    12901 ttgccgattt tgatgcactc tatgcataca atacggtgcc ggctaccgtt gcattccggg
    12961 atcattcaac cgggacaacg cccctgaact atgcatggga gtttggtgat ggcgcaacgt
    13021 caacagaaca aaatcccacc cacacgtttg tccgtaaagg gttatacacg gtaaaactca
    13081 cggtgacgaa cgtctacggg tcaagcactg aagcaaaaga gaattacatt gcaatcggtc
    13141 ttgctcccaa agcggatttt tccggctcgc caacaaccgg caatactccg ctgaccgtgg
    13201 agtttaccga tcggtcgacc ggttacccga caacctggaa atggaatttc ggtgacggaa
    13261 aagaatcatc ggacccaaat cctgtccaca cattctggtc gagcggcgat tataccgtta
    13321 ccctcactgc atcgaatgag tatgggacat ctgatgtaac aaaagcctac ctcattcacg
    13381 tgatacctgc gctcaaagcc aaatttatcg ctgatccaaa gcagggaaaa gcaccgctgg
    13441 ttgtgaagtt ttctgacctg
//
LOCUS       DASRLV010000113         7993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_524853,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000113 DASRLV010000000
VERSION     DASRLV010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7993
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524853"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VFC43_04555"
     CDS             <1..237
                     /locus_tag="VFC43_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK30488.1"
                     /translation="GVFIEIGWLPNTDILDGFLALNEKKEIIVDINGHTSMAGVFAAG
                     DVTSVKSKQIIIAAGDGAKAALEANEYVMKSATR"
     gene            complement(320..1789)
                     /gene="tfrB"
                     /locus_tag="VFC43_04560"
     CDS             complement(320..1789)
                     /gene="tfrB"
                     /locus_tag="VFC43_04560"
                     /EC_number="1.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043830 - thiol-driven fumarate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (CoM/CoB) subunit TfrB"
                     /protein_id="HZK30489.1"
                     /translation="MREITARVYRFDPEKDADAHFDEFTVKVNEGARVLHVLHAIHDT
                     RDPTLAYRYSCGSGQCGSCAVRVNGEPVLACMEEAQNNIIIEPLHLQVKKDLVVDLLP
                     SLETIASLKPSDTLKMPTKSDIDTIKPLRDCIECLCCVSVCPAMDVTQFLGPTAMRQE
                     MRLALDPRDRGDRIVDAVRDGLFTCTSCQACWKACPKKIEIPSKAIEKLRSLANKRGF
                     TLPKHQEVAALIRDTGRSVNRIEPTFMERVNAEVIEPAVPVKATVGFFVGCMYNMRLP
                     DAALDAIEVLRRNGIRIIIPKEQVCCGSPLIRTGQLTFLETLMKRNIDAFRFRNIDAV
                     MTMCAGCGSTLKNDYKTPFKVMDISEVLTTYGIEPPARLSVEATYHDPCHLLRGQGIR
                     DEPRTLIKQVVNLVEMPSICCGSGGGVRSGNPEEAAALGKRRGEEIKKSGAEIVISSC
                     PFCEFHIAGHTDKPVKNIATVLLEGYKEKDRKKAPAIVS"
     gene            complement(1786..3414)
                     /gene="tfrA"
                     /locus_tag="VFC43_04565"
     CDS             complement(1786..3414)
                     /gene="tfrA"
                     /locus_tag="VFC43_04565"
                     /EC_number="1.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043830 - thiol-driven fumarate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (CoM/CoB) subunit TfrA"
                     /protein_id="HZK30490.1"
                     /translation="MLADDVVDCHVLVIGSGGAGVRATIEASQYGDTVLISKTIVGKG
                     GCTTMAEGGYNAVMREQDSCGIHFEDTLKGGAYLNDPGLVNILVQEAPLRMSDLIQWG
                     AVFDFTENDEVAQRPFGGQRFPRTCYAGDRTGHEMMTTLVERLDSSDVTQFQEYTVID
                     LLKNGESVIGAMALDEKGNLILLKADSTILATGGGTKVYDISTNSSSGTGDGFAIGYR
                     AAAELIDMEMIQFHPTGAVFPYDARGRLVTEAVRGEGGILLNNIGERFMNKYDPERME
                     LSTRDVVARAIATEIQSGRGTKNGGVYLDVTHLPRTQIETRLPVMLEQFLKFGVDIRD
                     QPMEVAPTAHHIMGGLRITAECRTTIPGLFACGEVAGGVHGANRLGGNALAETQVFGK
                     RAGEFAGKTEKRMKKVDENQISQQLAFLDSLLKGSENPARVRMQLQQAMWQGAGIFRN
                     APDLNKTLETINNLSSAHIKAESPRNLAECCIVRNMMLTASHICRSALIRTESRGAHV
                     RIDIPQTYEARLSPYSHTFISLSRHGIEKNTGVS"
     gene            3476..4876
                     /locus_tag="VFC43_04570"
     CDS             3476..4876
                     /locus_tag="VFC43_04570"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HZK30491.1"
                     /translation="MRLLMKFGGTSVADANCISNVVDILEKHHKAGDELAVVVSAQRG
                     VTDQLLEVIGKLPTAKDDTAITPLIQSLGKRHMTTLEGAAPDYIAETGALIEERLISL
                     QNILFAVYNLKELSPRSKDYIISFGERLLAPIMSAALRQRGIPSTVMDGCEAGILTTP
                     QHGESTALLESDDRIKKMTGPLLEKEIPVIMGFMGCTRNGIVTTLGRSGSDYSASIIG
                     AGIDADEIWIWTDVDGIMTCDPRVINDARVIQSLSYIEVMELSYFGAKVMHPRSIEPA
                     MRKNILVRVKNTFNPSHPGTVIVRNGQKDNRVVKALTYIDKVAAININGAQMIGRPGV
                     AKAIFTILADQEVNVMMISQGSSEANISLIVDEAHLNTAVHALSDLVKQGVVREVTHN
                     HDVCAVAVVGAGMAGAAGTGGRIFTALGKTGVNVMMISQGSSEANISFVVNQMDGPRA
                     VRILHDEFRLSEESDE"
     gene            4869..5861
                     /gene="purM"
                     /locus_tag="VFC43_04575"
     CDS             4869..5861
                     /gene="purM"
                     /locus_tag="VFC43_04575"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HZK30492.1"
                     /translation="MSSTTYRDSGVDIDLEATAIKALIKNLTYKRKGCCTMLGSVGHF
                     AGLIDFGETTLALTTDGVGTKMLIADHMHDWSTVGIDCIAMNVNDLYVMNMEPVAFVD
                     YIATDKLSVEKMAQIGIGLNEGARQSNIDIVGGETASLRGLVNGLDLAGTCLGMQKKD
                     KIITGKSIQPGDKIIGVPSTGVHSNGLSLARSVVEKYASYDEELKNGRTIGQELLKPT
                     RIYHESLHVAETCTIHGMCHITGGGLLNFRRLSPHGFRFTTPLPPIEIFSWIQETGNV
                     STLEMYRTFNMGMGYSFIVPKDSAASVMKAVNGAQVVGEIVKEPGVWLRDIEIT"
     gene            complement(5866..6393)
                     /locus_tag="VFC43_04580"
     CDS             complement(5866..6393)
                     /locus_tag="VFC43_04580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZPR1 zinc finger domain-containing protein"
                     /protein_id="HZK30493.1"
                     /translation="MRTVVPGPCPYCNTEIEYIYQTENIPYFSDILIISANCSSCGYK
                     YVDTQLLIDKEPVRYEVVINSGEDLSIRIVRSMSASIEIPELGVRIDPGPACQGFVSN
                     VEGVLDRIEQILNGASLWGEEEERENARNLLARIAEVKTGAVPITLILEDRCGNSAII
                     SDKALVGAYKPEPDQ"
     gene            complement(6396..6764)
                     /gene="sepF"
                     /locus_tag="VFC43_04585"
     CDS             complement(6396..6764)
                     /gene="sepF"
                     /locus_tag="VFC43_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HZK30494.1"
                     /translation="MVKIIDTLLGKSSSHSDDDYMELDLASYEEHGGSAPALLVKIAT
                     ITDLKDTPRVKDEVYSGNIVIVDISRLKMDKISYERVLKDLKEVAKDVNGDIIGLGDQ
                     RYVVLTPMSVKISRDKIGGA"
     gene            complement(6888..7385)
                     /locus_tag="VFC43_04590"
     CDS             complement(6888..7385)
                     /locus_tag="VFC43_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HZK30495.1"
                     /translation="MKKIVVKKRHSIRKSQAQDLLSRLFDQIGDSAKLFHADMIEVLE
                     TNADVSLFMVHKKPLLMDTGNWVFPTLKGAIQFPFPERRVVVDAGAIPYVVNGADVMR
                     PGIVSVTDDVKGQAPVQIVDERHGKPLAIGIALLDAPDMRAGVSGKMCKNFHHVSDEI
                     WNIEI"
     gene            7569..7799
                     /locus_tag="VFC43_04595"
     CDS             7569..7799
                     /locus_tag="VFC43_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LSM domain-containing protein"
                     /protein_id="HZK30496.1"
                     /translation="MTKRPLDILDQVLNRQPVIVSLKGGREIRGILQGYDVHMNLVLD
                     KAEDVENGQVQKVGTLIVRGDNVIYISPSLEQ"
     gene            7811..7993
                     /locus_tag="VFC43_04600"
     CDS             7811..7993
                     /locus_tag="VFC43_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37e"
                     /protein_id="HZK30497.1"
                     /translation="MSKGTPSMGKMNKFTHIACRRCGKISFHAQKKVCSACGFGRSTK
                     MSSYKWHTKRPKIPTH"
BASE COUNT         2062 a         1957 c         1901 g         2073 t
ORIGIN      
        1 ggggtattta ttgagatcgg ctggttgccc aatacggaca ttctcgatgg attcttggca
       61 ctgaatgaaa aaaaggaaat catcgtggat attaatggcc atacaagcat ggccggagtt
      121 tttgcagccg gggatgtaac ttcagtgaaa agcaagcaga taattattgc agccggggac
      181 ggggcaaaag ctgcacttga agcgaatgaa tacgtgatga aatctgcaac ccgttaggaa
      241 aaatatcctc attttctcgt tttttaaaaa gaaaaacaaa agagatctga ttgaatttca
      301 ttcttttgcc agattttttt cacgaaacga tcgcaggagc ttttttccgg tctttttcct
      361 tatatccttc gagcagtaca gttgcgatat ttttcacggg tttatccgta tgcccggcga
      421 tatgaaactc acaaaatggg caggatgaga taactatctc agcaccgctc tttttaatct
      481 cttcacctct ccgcttcccg agagcagctg cctcttcagg attgccggaa cgtactccgc
      541 ctccggaacc gcagcagata gaaggcattt ccacaagatt cactacctgc ttaatcagcg
      601 tacggggctc gtcccgaatc ccctgtcccc gcaggaggtg gcagggatca tgataggtag
      661 cttctaccga tagtctggcg ggtggctcta tgccatatgt tgtaagcact tcagaaatat
      721 ccataacctt gaacggtgtc ttgtaatcat tcttaagagt cgaaccgcac ccggcacaca
      781 tggtcataac cgcatcgatg ttgcgaaacc ggaatgcatc gatattgcgt ttcatgagtg
      841 tttccaggaa cgtaagctga cccgtgcgga tgagcggaga accgcagcag acctgttctt
      901 tcgggattat aatacggatg ccgtttctcc tgagaacttc gattgcatcc agtgcagcat
      961 cgggcaggcg catgttgtac atacaaccaa caaagaaccc gaccgttgcc ttcaccggga
     1021 cagcaggttc gattacttcc gcgttcacac gttccataaa tgtcggttcg atccggttaa
     1081 cggagcgccc cgtgtccctg ataagtgccg ccacttcttg gtgttttggc agggtgaatc
     1141 cccgcttgtt tgccagggat cggagttttt cgattgcttt agatggaatt tcaattttct
     1201 tagggcatgc cttccagcag gcctgacagc tggtacaggt aaacagcccg tcccgcactg
     1261 cgtcgactat cctgtcccca cggtcacgcg gatcaagcgc aagccgcatt tcctgccgca
     1321 ttgctgtagg ccccaggaac tgtgtaacat ccatagccgg gcagactgaa acacaacaga
     1381 gacactcgat acagtcccgc agcggtttta tcgtatcgat atcggatttg gtcggcatct
     1441 tcagcgtgtc tgaaggtttc agggaagcaa tcgtttcaag cgaaggaaga aggtcgacca
     1501 caagatcctt ttttacctgc aggtgaaggg gttcgataat gatattattc tgagcttctt
     1561 ccatgcaggc aagcaccggt tcgccattta cccgtacagc acagctgccg cattgccctg
     1621 aaccgcagct atacctgtac gcaagtgtcg gatcccttgt atcgtgaata gcatggagga
     1681 catggagaac acgggccccc tcgtttactt tgaccgtaaa ttcgtcgaaa tgcgcatccg
     1741 catccttttc gggatcgaat cggtatactc tggcagtgat ctctctcatg atacccccgt
     1801 gtttttctct atcccgtgtc tggagagcga gatgaatgta tgactatagg gagagagccg
     1861 ggcctcatat gtctggggaa tatctatccg tacatgcgcc ccccgggact cagtcctgat
     1921 aagagcactc ctgcagatat gcgaagcggt cagcatcatg ttccggacaa tacagcattc
     1981 agcaagattt ctgggagatt ctgcctttat atgagcagat gacaggttat tgatcgtttc
     2041 cagggttttg ttgagatccg gcgcatttcg aaagatacct gcaccctgcc acattgcctg
     2101 ctggagttgc attcgaaccc gggccgggtt ctcacttccc ttcaggagtg aatcaagaaa
     2161 ggccaattgc tggctaatct ggttctcatc tacttttttc atccgttttt ccgtttttcc
     2221 tgcaaattcc ccggcccgtt tcccaaagac ctgtgtctcc gcaagagcat taccaccgag
     2281 acgattggca ccatgaacac ccccggcaac ctcaccgcag gcaaagagcc cgggaattgt
     2341 tgtccggcac tctgctgtta tccgtaaccc tcccatgata tgatgggcag tgggggccac
     2401 ttccataggc tgatcccgta tgtccacacc gaatttcaag aactgttcga gcatcaccgg
     2461 gagccgggtt tcgatctgcg tacggggaag atgggtaaca tcgagataga ccccgccatt
     2521 ttttgtgccc cgtccacttt gtatttccgt tgctattgcc cgagccacta catcacgcgt
     2581 ggaaagctcc atccgttcgg gatcatactt gttcatgaac cgctcaccga tgttattaag
     2641 gaggatcccg ccctcaccac gcacggcttc ggttacaagt ctcccccttg catcgtaggg
     2701 gaacaccgca cctgtcgggt gaaactgaat catttccata tcaatcaact cggcagctgc
     2761 acgatagcct atagcaaaac cgtctccggt cccactcgaa gagtttgtgg agatgtcata
     2821 tacttttgtc cccccgccag ttgcaaggat cgtgctgtct gccttcaata agatgagatt
     2881 gcctttctcg tccagagcca tcgcaccaat gaccgattca ccatttttca gcaggtcgat
     2941 aaccgtgtat tcctggaact gggtgacatc tgacgaatca agccgttcga caagtgtggt
     3001 catcatctca tgacctgtcc tgtcccctgc gtagcaggtc ctcgggaacc gctggcctcc
     3061 gaacggtctt tgggctacct catcattttc agtaaagtcg aacaccgctc cccattggat
     3121 cagatccgac attcgcaacg gtgcttcctg aacgagaata tttaccagtc cgggatcgtt
     3181 gagatacgct ccccctttga gcgtatcctc gaaatggatc ccgcacgagt cctgttcccg
     3241 catgactgca ttataaccgc cttctgccat tgtggtgcat cccccttttc ccacaatggt
     3301 cttggaaata agcacagtgt cgccgtattg cgatgcctct atggtggccc ttacaccagc
     3361 accaccgctt ccgattacca gcacatggca atccaccaca tcatctgcaa gcatcttatt
     3421 attttgtgga tcgtatagtt acataaataa atggcacagc caaagtgaga gagaaatgag
     3481 actattaatg aaattcggcg gaacttcagt tgccgatgct aactgcatca gtaatgttgt
     3541 ggatattttg gaaaaacatc acaaagccgg agatgagctt gcagttgttg tttctgcaca
     3601 acggggtgtg actgaccagc ttcttgaggt catcgggaaa ctccccaccg caaaggatga
     3661 taccgccata acaccattga tccagtcttt gggaaaaagg catatgacca cgcttgaagg
     3721 ggcggcccct gattacattg ctgaaactgg tgctttaatc gaggagcgtc tcatcagcct
     3781 gcagaacatt ctctttgccg tgtataatct gaaggagctc agtccccgtt ccaaagatta
     3841 catcatttcc tttggggagc gcctccttgc ccccattatg agtgcagcac ttcgtcagcg
     3901 cggtattcct tcgacagtaa tggacggatg tgaagccggg atccttacta cccctcaaca
     3961 cggcgaatca acggcacttc ttgaaagtga tgatcggata aaaaaaatga cagggccgct
     4021 cctggaaaaa gaaattccgg tcatcatggg attcatgggt tgtaccagaa acgggatcgt
     4081 gacgactctc ggcaggagcg gttcggatta ctccgcatct ataatcggtg ccggaataga
     4141 tgcagacgag atctggatct ggactgacgt tgacggtatt atgacctgcg atccaagagt
     4201 tataaacgac gctcgggtca tacaatccct gtcttatatt gaagtgatgg agctctcata
     4261 tttcggtgca aaagtgatgc atccgcgatc gattgaaccg gccatgcgga aaaatatcct
     4321 ggtccgggtg aaaaatacct ttaatccctc tcatccggga acggtaatcg tacgaaacgg
     4381 gcagaaggac aaccgggtgg ttaaggccct gacctatatc gacaaggtcg ctgcgataaa
     4441 tatcaatggt gcacagatga tcggaagacc cggtgtggca aaagcaattt tcaccatact
     4501 tgcagatcag gaggtcaacg tgatgatgat atcccagggt tcatctgaag caaatatctc
     4561 gctgattgtg gacgaagcac atctgaatac tgcggtccat gccctctccg atctcgtaaa
     4621 gcagggtgtt gtacgtgaag ttacgcataa tcatgatgta tgtgctgtgg cagtcgtcgg
     4681 tgccggaatg gctggggcgg cgggcactgg aggcaggatt ttcaccgccc ttggaaagac
     4741 cggtgtaaat gtgatgatga tatcccaggg ttcatctgaa gcgaatatct catttgtggt
     4801 gaatcagatg gatggtccac gggctgtacg tattctccac gatgagttca ggttatcgga
     4861 ggaatccgat gagtagcaca acataccgtg attctggcgt agatattgac cttgaagcga
     4921 cagccattaa agcccttata aaaaatctga cctacaaaag aaaaggctgc tgtacaatgc
     4981 tcggtagtgt gggtcacttt gcaggactca tcgattttgg ggaaactacc ctggcgctga
     5041 ccaccgacgg agtcggtaca aaaatgctga ttgccgatca tatgcatgac tggagtacag
     5101 ttggtataga ctgcattgcg atgaatgtca atgacctgta cgtgatgaat atggaacctg
     5161 tcgcgtttgt ggactatatc gccacggata aactctctgt tgagaagatg gcgcagatag
     5221 gcattggatt aaatgaaggc gcccgacagt caaatatcga tattgttggt ggtgagactg
     5281 cgtctctcag aggtcttgta aacggtctcg atcttgcagg cacgtgtctg ggaatgcaga
     5341 aaaaggataa aattattaca ggaaaatcca ttcagcccgg ggacaaaatc atcggagttc
     5401 catcgacagg tgttcatagc aacggccttt cgcttgcccg cagtgttgtg gaaaaatatg
     5461 ccagttacga tgaggaatta aaaaatggca gaactattgg tcaggaactc ctcaaaccaa
     5521 cccgtatata ccatgaaagc ctgcatgtag ccgaaacctg cactatccac ggcatgtgcc
     5581 atataaccgg tggagggctg ctcaacttca ggcgactcag cccgcatggg ttccggttta
     5641 ctacccctct cccccccata gaaatcttct catggatcca ggaaacagga aatgtctcta
     5701 ctcttgagat gtaccgtacg ttcaatatgg gtatgggata ttctttcatt gtcccgaaag
     5761 acagcgctgc aagcgtgatg aaagctgtca acggtgcaca ggtagttggg gaaattgtaa
     5821 aagaacctgg cgtgtggctt cgggatatcg aaattacata acccgttatt gatctggttc
     5881 gggtttatat gccccgacca gtgctttatc tgaaataatc gcactgttgc cacaccgatc
     5941 ttcaaggata agtgtaatag ggactgctcc ggttttcact tcggcaatcc tcgccagcag
     6001 gttccgtgca ttttcccgtt cctcctcctc cccccagagg gatgcaccat tcaggatttg
     6061 ttctattcga tcaagtacac cctcgacatt ggagacaaaa ccctggcatg ccggccccgg
     6121 atctatcctc acacccagtt cagggatttc gatgcttgca ctcatactcc tgacgattcg
     6181 gattgagagg tcttctcctg aattaattac cacctcatac cggaccggtt ctttatcgat
     6241 gaggagctgt gtatccacgt atttgtaacc gcatgaacta cagttcgcgg agatgatgag
     6301 aatgtcagaa aaataaggaa tattttctgt ctggtaaata tattcgattt ctgtattgca
     6361 ataaggacaa ggacctggaa cgactgttcg cacctttaag ctccaccgat cttatcgcgg
     6421 gaaattttta ccgacatcgg ggtaagcacg acgtacctct gatctccaag accaatgata
     6481 tcaccattaa cgtcctttgc tacttctttt aggtctttta aaaccctttc gtacgatatc
     6541 ttgtccatct tgaggcggga aatgtcaaca attacaatgt taccgctgta gacttcgtct
     6601 ttaacccgcg gagtatcctt gagatccgta atagttgcaa tctttacaag cagtgcggga
     6661 gcagatccac catgctcttc ataggacgca agatccagtt ccatgtaatc gtcatcggag
     6721 tgcgaggaac tctttccaag aagggtgtca atgattttaa ccataaaaaa actgtatgat
     6781 gaaacttatt taatagtatc tatttggaaa gcatctatcc gggttttttt accataaaat
     6841 gggaaaaacg accggttttc cgcataatta ataaagagcg gtttatgtca gatctctatg
     6901 ttccatatct cgtcgctgac gtgatggaaa tttttacaca tctttcctga aacaccggca
     6961 cgcatgtcag gcgcgtccag gagcgctatt ccaatagcaa gcggtttacc atgccgctca
     7021 tcaacaatct ggaccggggc ctggccttta acatcatcag taacagaaac gatacctggc
     7081 cgcatgacgt cagcaccatt aaccacataa ggtatagctc ccgcatccac cacgactctg
     7141 cgttcgggaa acggaaactg gatggcgcct tttaatgtcg gaaataccca gtttcccgta
     7201 tccattaaaa ggggtttttt gtggaccata aacaatgaaa catcggcatt ggtctcaagg
     7261 acctcaatca tatctgcatg aaaaagttta gctgaatcgc cgatctgatc aaaaagcctg
     7321 gataaaaggt cctgcgcctg acttttgcgg atggaatggc gttttttcac aacaattttt
     7381 ttcatcgtta cagttcctac attcatatct ctcgcttaaa aataaattgt atcggaaaat
     7441 tttcgtattt gaaaaatata tatttaaaac ggtgggaaaa tttattggct gatgcagatc
     7501 tacggtatgt ttaagtaagc agctcacaca catatattac tccagagcga tggtagcagg
     7561 gtattattat gaccaaaaga ccgttggata ttttggatca ggtgctgaac cggcaacccg
     7621 tcattgtatc tctcaaaggt gggagagaaa tacgtggtat tctccaggga tatgatgttc
     7681 atatgaacct tgtgctggat aaggcagagg acgtggagaa cggacaggtt cagaaggtag
     7741 gtacgctcat cgtccgtggg gataatgtta tctatatatc cccctcgctg gaacaataag
     7801 gtgaagtaac atgtcaaaag gtactccgtc aatgggaaag atgaacaagt tcacccatat
     7861 cgcctgccgg cgctgtggga agatctcgtt ccacgcccaa aagaaagtct gctctgcctg
     7921 cggttttggc agaagcacaa agatgagcag ctataagtgg catacaaagc gaccaaaaat
     7981 cccgacacat tag
//
LOCUS       DASRLV010000114         4216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_525679,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000114 DASRLV010000000
VERSION     DASRLV010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525679"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VFC43_04605"
     CDS             complement(<1..656)
                     /locus_tag="VFC43_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZK30498.1"
                     /translation="MASEKPHLNLAVIGHIDHGKSTTVGRMMFETGAVPAHIIEGYRK
                     EAESKGKATFEFAWVMDNLKEERERGITIDIAHKRFDTPKFYFTVVDCPGHRDFVKNM
                     ITGASQADAAILVVAAPDGVMEQTKEHVFLARTLGITQIIIAINKMDAVKFDEKRFNE
                     VKKDLSDLIKMVGYKPDETLFIPISALGGQNIKVISPETPWYKGLPLITALDTFKSPD
                     "
     gene            823..1119
                     /locus_tag="VFC43_04610"
     CDS             823..1119
                     /locus_tag="VFC43_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30499.1"
                     /translation="MKTLPFQRDEKKNRVTVMCADGEISVHSHCAYCMHCNGIWVGKR
                     LVPSPQVQVLSEFKKGSANDDSLMNAAMMFNTLIRDGSSLECSDIENRGFKRLF"
     gene            complement(1252..2127)
                     /locus_tag="VFC43_04615"
     CDS             complement(1252..2127)
                     /locus_tag="VFC43_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZK30500.1"
                     /translation="MRTDFSIHMKGGIITERDADFCTIRVRAPAGILSVEKLRGIAVL
                     AKKYGSGIVHCTTRQTIEIPHVDPKSIKKIAKSLEKNGTPIGSERDEIVNIIACPGVD
                     RCKFANIDTINLAKKIDQKLFGKEMPVKIRIAISGCPNSCTSPMLNEIGIMGRIRPLR
                     SEGLCTGCGTCVEYCKEKAIIIKRGISVLDPAKCVQCGVCIRSCPFDLLKAEHRHYLI
                     LVGGRRGRHPRIGRELLTLEDEDQVIYVVEKLVTWVYRRAWSGRLLSEQLDDIQFEKF
                     RQEILHEISEKIARV"
     gene            complement(2181..2891)
                     /gene="cobS"
                     /locus_tag="VFC43_04620"
     CDS             complement(2181..2891)
                     /gene="cobS"
                     /locus_tag="VFC43_04620"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HZK30501.1"
                     /translation="MKPLISLLQFTTILPLGKPQDLGSFARYSYIYPCAGYIIGGMIA
                     LPVFFITDRIIASAVAIAALLLITGVHHFDGLLDLGDGLMAHGDKEKRIRALTDRQVG
                     AGGVAAGIAVTVLLFAGLMASSSIIVAVIIGEVCAKFSMAFLTAYGNPFREGIHSYLH
                     KFSKPFFPFIAALFCIPLVFLPVSPVQITGSLLAMVLCPVLLLIVSKRLFGGINGDIV
                     GASNELTRACVIVVIALI"
     gene            complement(2888..3322)
                     /locus_tag="VFC43_04625"
     CDS             complement(2888..3322)
                     /locus_tag="VFC43_04625"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HZK30502.1"
                     /translation="MFEIEQRLLEKGITIDDIVDAAMGLYVSHGMPDDQAAVEIRKKI
                     LKYLNDPNVSSLLLGAILLENELYHKRDNSEIADDPVFLLSDEIIGMAIAECIGGTYA
                     RFEFTRYDQKKPGILSVLGPFLDDAIAGLIAGCTSRLYSECV"
     gene            3423..>4216
                     /locus_tag="VFC43_04630"
     CDS             3423..>4216
                     /locus_tag="VFC43_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HZK30503.1"
                     /translation="MGFLSETPDIIFKRPLFCGILANTLLSTVPGVSGAGATPEKTLL
                     TPVLDAELITTGAITSHKARPDTPTGCPTPASITRAMMELCGIQPVFINAGLRYTPTV
                     PCFDIYGKPGEDPRFRDAVPHVLDLVKKGESLGKHLSKCSDLLILGECVPGGTTTALC
                     VLRGLGYEASVSSSFVNNPVTIKEQICKVSLQRIKAGEITSPLDIIRYTGDPMMPVAA
                     GIAKTYDGSLILAGGTQMLSVCAVIKGLGGSLPFVVTTSYVRDDPSA"
BASE COUNT         1145 a          967 c          959 g         1145 t
ORIGIN      
        1 ttatctggtg acttgaatgt gtcgagagca gtaataaggg ggaggccttt gtaccagggt
       61 gtttcagggc tgataacctt gatattctga ccaccaagag cgctgatggg gatgaaaaga
      121 gtctcgtcag gtttgtaccc aaccattttg atgagatcag agagatcttt tttcacttca
      181 ttgaagcgtt tctcgtcgaa ttttactgca tccatcttgt tgattgcaat aatgatctgg
      241 gtgattccaa gggttctggc gaggaagaca tgctcctttg tctgttccat aaccccatcc
      301 ggggctgcta ctacaagaat tgcagcgtca gcctgtgatg caccggtaat catgttcttg
      361 acgaagtctc tatgtcccgg acaatctaca accgtgaaat aaaatttcgg cgtatcaaat
      421 cgcttgtgtg cgatatcaat ggtgattcca cgttcacgct cttcttttaa gttgtccata
      481 acccaggcga attcaaaggt agccttaccc tttgattcag cctccttccg gtaaccttca
      541 atgatgtgtg caggtactgc accagtctcg aacatcattc tcccgacggt tgtagactta
      601 ccgtggtcaa tatgtccgat tacagccaga ttcagatggg gcttttcaga tgccattttg
      661 ttcctccaaa tgttgtggga ctgtccgctt acagtcagtt tatgcgagta ttacatatat
      721 gagacccatc tatataaatt atttcgattc tggaaaactg ttttccgata taaaaatata
      781 tatcgacact atgataacag atgccaaaaa taccttctca tcatgaagac attgccattc
      841 cagcgagatg aaaaaaagaa ccgggttacg gtgatgtgtg ccgatggtga aatttccgtg
      901 cacagccatt gcgcatactg catgcattgt aatggtatat gggtgggaaa acgtctcgtc
      961 ccctccccgc aggttcaggt gctgtcagag ttcaagaaag gttcggcaaa tgacgattcc
     1021 ttgatgaacg cagctatgat gttcaatact ctgatacgtg acgggtcctc gcttgaatgc
     1081 agcgatatag agaaccgtgg gttcaagaga ttattttaaa aaatacaaga gaaaatacca
     1141 gaagtcccgg ttttttcatt aaatcatttt tgaaaatgga atgaatgttt taagtttacc
     1201 tgcccttgag atacctgata gcgatacgtg taaaagtcca taaggaaaaa atcatacccg
     1261 ggcaatcttt tcggagatct cgtgtaaaat ttcctgacgg aatttttcaa actggatgtc
     1321 atcaagttgt tctgagagca accttccgct ccacgcacgg cggtaaaccc aagttacgag
     1381 tttttccaca acatagataa cctggtcttc gtcttcaagt gtgagcagct cccttccaat
     1441 gcggggatgt cgtcctcttc ttcccccgac aagaatcagg taatggcggt gttctgcctt
     1501 caggagatca aaaggacacg atctgatgca tactccgcac tgtacgcatt ttgcaggatc
     1561 aagaaccgaa atccctcgtt tgataattat ggctttttct ttgcaatatt ccacgcaggt
     1621 gccacaaccg gtacaaagtc cttcagacct taatggccgg attcttccca taattccaat
     1681 ttcgttaagc attggactcg tgcaggaatt cgggcacccc gatattgcaa ttcggatttt
     1741 taccggcatc tccttgccga acaatttttg atcgattttt ttcgcgagat ttattgtatc
     1801 aatattggca aatttgcagc gatcaacacc aggacatgca atgatattga caatttcatc
     1861 acgctcagac ccgataggag taccattttt ctccagagat ttggcaattt ttttgattga
     1921 ttttgggtct acatgaggaa tctcgatggt ctggcgtgta gtgcagtgaa ctattccgga
     1981 accatatttt tttgcaagta ctgcaatgcc acgtaacttc tctactgaca agatacctgc
     2041 cggtgccctt acccgtatag tacaaaaatc tgcatctcgt tcagttatga taccgccctt
     2101 catatgaatg ctgaaatcag tacgcatcaa acagtaatgt ggggaatctg atactaaaag
     2161 atgctgttgt taccgtaagg tcagataagt gcaatcacga caataacaca tgcacgggtc
     2221 agctcgtttg aggcacccac gatatctcca ttaataccgc cgaacaatct ttttgaaaca
     2281 ataaggagaa gaaccgggca taaaaccatg gccagtaatg atcctgttat ctggacaggc
     2341 gaaaccggga gaaacacgag aggtatgcag aaaagcgctg caatgaacgg gaagaacggt
     2401 tttgaaaatt tgtggagata actgtgaata ccctctctga aggggttgcc ataggcggtt
     2461 agaaatgcca tggaaaattt ggcacagact tcaccgataa ttactgcaac gataattgat
     2521 gacgatgcca tcagacctgc aaataataaa acagtgactg caattccggc agcaacgcct
     2581 cccgctccaa cctggcggtc cgtcagggca cggatccttt tttccttgtc cccgtgagcc
     2641 atgagaccgt ccccgagatc gagcaatccg tcaaaatgat gtactccggt aatcagaagt
     2701 aatgcggcaa tcgccacagc ggatgcaatt atccggtctg taatgaaaaa aaccggtaat
     2761 gcgatcatcc cgccgatgat ataccctgca caaggataga tataagaata tctggcaaaa
     2821 ctcccgagat cctgcggttt accgagcggg agaatggtag taaactggag cagggagata
     2881 agaggtttca tacacattcg ctatacaggc gtgaggtaca gccggcaatt aaccctgcga
     2941 tggcatcgtc cagaaaaggt ccgagaactg ataagattcc gggctttttc tgatcgtacc
     3001 gggtaaactc aaaccgggca taggttccgc cgatgcactc agcgattgcc attccgatta
     3061 tctcatcgct caacagaaat accgggtcat cagcgatttc ggagttgtct cttttgtgat
     3121 acagctcatt ttcaagcagt attgccccga gaagcaggga agaaacattc ggatcattaa
     3181 gatatttaag aatttttttc ctgatttcaa cagctgcctg gtcatccggc atcccatgag
     3241 aaacgtacag gcccatggcc gcatcgacga tatcatcaat ggtgatgccc ttctcaagta
     3301 accgctgttc gatttcaaac atgtgcatag gaatgtgagt taccggacta ttatatcgtt
     3361 gtggactctt gtgatccgat atcttcaaag tgccagggtg agcaatatta gaatagattg
     3421 tcatgggatt tctctcggaa acaccggata ttattttcaa acgccccttg ttttgcggta
     3481 tccttgcgaa cacattgtta tctacggttc ccggtgtttc gggagcagga gcaacaccgg
     3541 aaaagactct tctcacacca gtccttgacg cggaactgat taccactggt gccataacga
     3601 gtcataaggc aaggcccgat acaccgactg gttgcccgac cccggcttcc atcacccggg
     3661 caatgatgga actatgtggt atacaacctg ttttcatcaa tgccggcctc cgttacacac
     3721 cgacggttcc gtgttttgat atatatggta aaccaggcga ggatccaagg ttccgcgatg
     3781 cagttcccca tgttctggat cttgtaaaga aaggagaatc tcttggcaaa catctttcaa
     3841 aatgcagcga tttgcttatt ttgggcgaat gtgttcccgg gggaactaca acagcgttgt
     3901 gtgttttaag gggacttggc tacgaggctt ccgtcagcag cagttttgtc aataatccgg
     3961 taaccataaa agaacaaatc tgcaaggtgt cactccaacg aataaaggca ggtgaaatta
     4021 catcaccgct cgatattatc cggtacaccg gcgatcccat gatgccggta gctgcgggaa
     4081 tagcaaagac ctatgacgga tcactgatat tagccggcgg tacccagatg ctgtctgtct
     4141 gtgctgtcat aaaagggctc gggggatccc tgccgtttgt tgttactacc tcctatgtga
     4201 gagatgatcc ttcggc
//
LOCUS       DASRLV010000115         8708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_526146,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000115 DASRLV010000000
VERSION     DASRLV010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 8708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8708
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526146"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..249)
                     /locus_tag="VFC43_04635"
     CDS             complement(4..249)
                     /locus_tag="VFC43_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30504.1"
                     /translation="MPSYPILRESFACDTARETVLALGAASPTDGIRVAKEDGWFLIR
                     ASGTEPKIRITAEAKTLLKAKEMLKEGRNLVKQGKTA"
     gene            complement(277..1254)
                     /locus_tag="VFC43_04640"
     CDS             complement(277..1254)
                     /locus_tag="VFC43_04640"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase"
                     /protein_id="HZK30505.1"
                     /translation="MLFGSSGIRRKFDQTLIDTALSVGSALAFRSSDIVIGMDTRTTS
                     PLLVHLVTSGILGSGGTAYNTGIAPTPSVAYSTRTVKAGCMITASHNPEEYNGLKLFN
                     PDGSSFTQGQQAEMEVLLKARYWTDWQHQGSERSIDALTPHKNAILDSVHIKKGIAVI
                     VDCGNGAGSVLTPHLLAAAGAKIHSINCNPSGHFARPSEPLKENLGYVGEMVKKTGSS
                     CAVVHDGDADRMMAFDNKGRYIDGDHLLMLFARYLNAKQVVTTSDASMIIEDIAEVKR
                     TPVGDTYVSEELLKWGDFGGEPSGAWIFPQISYCPDGPFAAALFCEIAS"
     gene            complement(1648..1962)
                     /locus_tag="VFC43_04645"
     CDS             complement(1648..1962)
                     /locus_tag="VFC43_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30506.1"
                     /translation="MMDLIIDTVNKLETMIKDSGCEDSGVVLDVRYDTVNCQFERGAC
                     MTAVFGGRSADFVTFDPIRAKTKISFMFGAPLDTPPVRGAAAAIVNVAMGFFCLSRVL
                     HS"
     gene            complement(1959..2585)
                     /locus_tag="VFC43_04650"
     CDS             complement(1959..2585)
                     /locus_tag="VFC43_04650"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HZK30507.1"
                     /translation="MGRSVKIIDITRPLSEDCLTYPGDISPVFRQADHGNYLITDLHM
                     STHTGTHVDAPVHYLKMGDTIDTIPLSQIIGTCRVLDVTRSGTRIAANDLIGRLDGIE
                     RLLLKTSFSGTESFDENYPSLTEDAALLITSLEMKCVGIDSPSIESFGGEGNVHCELL
                     GHGCIIIELLDLTEVEEGDYTMIALPLRLTGLDGSPARVVLIDNNGCE"
     gene            complement(2578..3228)
                     /locus_tag="VFC43_04655"
     CDS             complement(2578..3228)
                     /locus_tag="VFC43_04655"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HZK30508.1"
                     /translation="MVTYPRVDERIRMVESQIAARGISDERVLSVMKTIPRHLFIPPP
                     YDKSAYEDCPLPIGNGQTISQPFIVALMTELLILQSADSVLEIGTGCGYQAAVLAALV
                     RTVTTIERIPEVAGLARANLMAVSSKNVEVIVGDGTLGYPTKAPYDGIIITAATPSIP
                     RPLIEQLAEGGRLVAPVGGRDIQELIRIRKNDGLIKEEQHGGVRFVPLIGECGWNG"
     gene            complement(3370..4356)
                     /locus_tag="VFC43_04660"
     CDS             complement(3370..4356)
                     /locus_tag="VFC43_04660"
                     /inference="COORDINATES: protein motif:HMM:NF013614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HZK30509.1"
                     /translation="MPSPVKVPELSKADQDRVAQAGIDLAMKNRCGSYLQMDQDIVQA
                     ECSQQGIEVLSFKKAMEKYNGMKEYTWQAVSPEKDEITRYVAAQKDPKGYVIIAHKGS
                     NNILPIQACLYLGREQIQHVHNIIVAEEGAELHIISGCTSGAHVGKGGAHYGISEFFV
                     KKDAKISFTMIHNWSENIEVYPRSASIVEENGVFLSNYVCMQPVKKIQMYPTATLKGA
                     NSVARFSSVVISTPGSYMDLGSRAILEGKGSSAELITRAITKGGTIISRGHINGKTAG
                     TRGHLECKGLILNDGIIYAIPEIEGSVVGTELSHEAAVGKLARDEIEYLMSR"
     gene            complement(4341..5072)
                     /locus_tag="VFC43_04665"
     CDS             complement(4341..5072)
                     /locus_tag="VFC43_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK30510.1"
                     /translation="MLHIEDLHVKIGDKEVLHDINLHIGEGETHVLLGPNGSGKTTLL
                     MTVMGFTNYQITEGKILFKGEDVTTMKAHERAKRGIGMLFQRPPTISGLKLGKMLTAI
                     SKNSEDNIPELAKSVHMDKFLERDINKGFSGGEIKRSEVLQLMIQNPDFIMLDEPESG
                     VDLENISLIGTAIGSLLEKDKHLIKRKKSGLVITHTGYILDYLDADKGHVMCEGEIKC
                     HGNPREILKDIKQRGYKECLECRHQ"
     gene            5399..6313
                     /locus_tag="VFC43_04670"
     CDS             5399..6313
                     /locus_tag="VFC43_04670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HZK30511.1"
                     /translation="MRPEKVRDKAGKAFIRKGSKGAKKSVISSAPREILPFVNHIICA
                     DALHALACIPDQSIDLIITSPPYNFGHAYAHDPHDDTHEWNQYFENLLGIWQECVRVI
                     RPGGRIAVNIQPLFSDYVPTHHLISHQLAGLGLLWKAEILWEKNNYNAKYTAWGSWKS
                     PSMPYIKYTWEFIEVFDKETHKKCGRQEDIDITAEEFKEWVLGRWSFPPEIHMKDYNH
                     PAMFPEELPRRLMKLFSYKNDIVLDPFNGAGTTTLVAWKLHRRFIGIDVSDQYCETAF
                     QRLASEAENCAPTSPEYPLPSMMVMEHE"
     gene            complement(6334..7458)
                     /locus_tag="VFC43_04675"
     CDS             complement(6334..7458)
                     /locus_tag="VFC43_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30512.1"
                     /translation="MHNRFLKLTGIFLLIIILVLSTVLISAVVFPKVTIIVPHIAPDL
                     SSLKTFPLNISHQFPFENTTVVLTIPINVSVYEGAMQAEKATTVYGNISETVWLAQSY
                     RAMVQDPAQESLYAALLAETDKIRLRQKLSDDEYLELIAVYIQSLKYETREQNPAKFP
                     IETVVDRAGDCDDKSLLLAGLLSRAGYPVALLLFGPESHMALGVGSDEYIYKNTGYAF
                     LETTNYSFVGVPTDTLGANLTLYSDPVIIPISNGTKTYRSGSETRYIHEMYVLSNRKV
                     KELEPHVKSMSEDITSQQEKIARLETEMQDFKTTRNTGRYNAQVPVHNALVEEYNSRL
                     DTYRQIFTLYEKYALIHNYILEHIYDRKGVYEYVKKTMPA"
     gene            complement(7497..8267)
                     /locus_tag="VFC43_04680"
     CDS             complement(7497..8267)
                     /locus_tag="VFC43_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HZK30513.1"
                     /translation="MIDVTGFLIDLDGVLYVGDHAIKGARETIDLLRDRKYTLRFVSN
                     TTRKCRKTLAEKLSSLGFDIPVEHIFTPPLAAVACMKNTGKHHCYLLTTGDVCRDFEH
                     ECICDSVSGIEVVIIGDAGEKITYDSLNTAFRHLMNGADMIALEKDRYWMAPGGLSLS
                     AGPFVQALEFATGKKATVVGKPSRKFFALALSDMGLCAGQVAMIGDDILTDIGGARTM
                     GMQGVLVRTGKYRKDAVDNAVIKPDYVIDSIARLRDIL"
BASE COUNT         2125 a         2141 c         2069 g         2373 t
ORIGIN      
        1 catttaagca gttttcccct gcttaaccag gttacgacct tcttttagca tctcctttgc
       61 tttcaggagg gttttggcct ctgcggttat gcggatttta ggctcggtcc cgcttgcccg
      121 gatcaggaac cagccgtcct cttttgcgac cctgattcca tccgttggac ttgccgcccc
      181 aagagccaga acagtttccc gtgcggtgtc gcaggcaaaa gattcccgca ggatcgggta
      241 ggagggcatt gcatcaagct cctcggagat attccattaa gatgcaatct cgcagaaaag
      301 ggctgcagca aatggcccat cgggacagta tgagatctgc gggaaaatcc atgctcctga
      361 gggttcaccc ccgaaatccc cccacttcag gagttcttca gacacgtagg tatcaccaac
      421 aggtgtcctt ttgacttcag cgatatcttc aatgatcatg gaagcgtcac ttgtcgttac
      481 tacctgtttg gcgttaagat accttgcaaa aagcatgaga agatggtccc cgtcaatgta
      541 gcgtcctttg ttatcaaatg ccatcatccg gtcggcatcc ccgtcatgca caaccgcaca
      601 ggaagatcct gtttttttca ccatttcccc cacataaccc aagttctcct tcaatggttc
      661 tgaaggacgg gcaaaatgcc ccgatggatt gcagttgatg ctgtggattt ttgcaccagc
      721 agcagcaaga agatgcgggg taagcacgct ccctgcaccg ttcccgcagt caacaattac
      781 ggcgattccc tttttaatat gaacactatc aagaatggca tttttatgag gggtaagtgc
      841 atctatcgac cgctcactac cctggtgctg ccagtcagtc cagtaccggg ccttgagaag
      901 tacctccatc tcagcctgct ggccctgggt aaacgaagaa ccatccgggt tgaaaagttt
      961 caatccgtta tattcctcag ggttatggga tgcggtgatc atacacccgg cttttaccgt
     1021 acgggtacta tatgcaacag acggagtcgg ggcaatcccg gtattatatg ctgttccacc
     1081 acttccgagg attccggagg ttaccaggtg tacaagaagg ggacttgttg tccgggtatc
     1141 catgccgata acaatatcgc tggaacggaa tgccagtgcc gaccccacgc tcagggccgt
     1201 gtcgataaga gtctgatcaa acttccttcg tattcccgaa gacccgaaaa gcatgatgat
     1261 ttattagatc gcaatcaaaa agagtttacc ttttttgccc tgatcgtttt catgtcatca
     1321 gtttttttgg agccggggtc tgccatgtcg tcagcaggta ccaaaagggc accagagctc
     1381 gatccggttg atccgtgcaa caccagcggt tgacggacca atacacagga tcctcgttgt
     1441 ttctttagcg ttccttatga tagcatctgt acccggctct atgattccct gaccgtggat
     1501 gaggataacg tctgcgtcct gtggatttgg aacaatagca ctcctgaagg tactttcaat
     1561 agcggggatg ctgcctgtac aatagatccg ttttcctcca agtgtaagga gcagttgatc
     1621 gcgacactga gtgtgggaag attcgggtca ggaatgaaga acccgtgaaa ggcagaaaaa
     1681 acccattgca acgttcacga ttgcagcggc agcaccccgc accggaggag tatcgagagg
     1741 tgcaccgaac atgaacgaga tctttgtctt tgccctgatg ggatcaaatg taacaaaatc
     1801 tgcacttctg ccgccaaata ctgcagtcat acatgcaccg cgttcaaact gacaattaac
     1861 ggtatcgtaa cgtacatcaa gaacaacgcc gctgtcttca cagccgctgt cttttatcat
     1921 cgtttcgagc ttattaacag tatcaataat gaggtccatc attcacatcc attgttgtca
     1981 atgagtacaa cccgggcagg agaaccatca agcccggtta agcgcagcgg cagtgcaatc
     2041 atggtataat ccccttcctc aacctcagtg agatcgagga gttcaatgat aatgcatcca
     2101 tggccgagca gctcacagtg tacatttcct tcgcccccaa aggattctat ggagggcgaa
     2161 tcgataccca cacatttcat ttcaaggctg gtgatgagaa gtgctgcatc ttccgtcagg
     2221 gaagggtaat tctcatcaaa actttcagtg ccggaaaatg acgtttttaa aaggagcctt
     2281 tcaatgccat ccaggcggcc tatcaggtca tttgccgcaa tcctggtgcc cgaacgggtc
     2341 acatccagta cccggcatgt tccgatgatt tgtgagagtg ggatagtgtc aatggtatcg
     2401 cccattttca ggtaatgcac cggagcatcg acatgagtcc cggtatgggt gctcatgtgc
     2461 aggtcagtga tcaggtaatt cccatgatca gcctgccgga ataccgggga gatatcacca
     2521 ggatatgtaa ggcagtcttc agagagtggg cgcgtgatat cgataatttt caccgatcta
     2581 cccattccat ccgcactccc cgatgagggg aacgaacctg actcccccgt gttgttcctc
     2641 ttttattaat ccatcatttt ttcttatccg tatgagctcc tggatatcac gtcccccgac
     2701 gggagctacc agcctgcccc cttcagccag ttgttcgatc aggggacggg gaatcgatgg
     2761 tgttgcagca gtgatgataa ttccgtcgta aggggctttt gtcggatacc cgagtgttcc
     2821 atcacccacg atcacttcaa cattcttact gctgacagcc atgaggtttg cccttgccag
     2881 acctgcaact tccgggatcc gctcaatggt cgttactgtt ctgacaagtg ctgcgagtac
     2941 tgcggcctgg taaccacatc cggtcccgat ctcaagcacg ctatcagctg attgcaggat
     3001 aaggagttct gtcatcaggg caactataaa gggttgggat atggtctggc cattacctat
     3061 cggcaggggg cagtcttcgt atgccgattt gtcatagggt ggaggaataa agaggtgacg
     3121 ggggatggtt ttcattaccg agagtacgcg ctcgtcactg atgcctctgg ctgcgatctg
     3181 ggactccacc atcctgatcc tttcatcgac tcgtggatat gtcaccatag gtattagatt
     3241 ttagaatccg gctttctctg ccacatcaat ggaggcatcg atctgtcttt gcagcgcatc
     3301 gggaagcccg gcaatcttca catccaaaaa tcccctgata atcgttgccg ttgcttcatc
     3361 ttcgtcaagt cacctggaca tcaggtattc gatctcatca cgtgcaagtt tacctacagc
     3421 cgcctcatga gagagctctg taccaacaac tgacccctct atctccggaa ttgcgtaaat
     3481 gataccatca ttcaggataa gtcccttaca ttcaaggtgc cctcgggttc cggctgtttt
     3541 tccattgatg tgaccccgtg aaatgatagt gccgcccttt gtaatggcac gggtaatcaa
     3601 ttctgcacta gagcctttgc cttcgaggat ggcacgggaa ccaaggtcca tgtatgaacc
     3661 aggtgtggag atgacaacac tactgaaccg tgcgaccgag ttggctccct tcagggtagc
     3721 cgtggggtac atctggattt ttttcaccgg ctgcatacag acataattgg aaaggaacac
     3781 tccgttttct tcaacaatgg aagcgcttct cgggtagact tcgatatttt cgctccagtt
     3841 gtggatcatc gtgaaactga tcttggcatc ttttttcacg aaaaactcag agatgccata
     3901 atgagcgccg cctttaccca catgcgcccc acttgtgcac cctgatatga tgtggagctc
     3961 agccccttct tcagccacaa ttatattgtg gacatgctgg atctgttcac gcccgagata
     4021 gaggcacgcc tggatgggga ggatattgtt agaacctttg tgcgcaatga tgacgtaacc
     4081 cttcgggtct ttctgtgctg caacataacg ggtaatctca tctttttccg gggatactgc
     4141 ctgccaggta tactccttca tgccgttata tttttccatt gccttcttaa acgagaggac
     4201 ttctatgccc tgttgggagc attcagcctg gacaatgtcc tggtccatct ggagatagct
     4261 gccgcatcgg tttttcatgg caaggtctat ccctgcctgg gctacgcgat cctgatcagc
     4321 ttttgagagc tcagggactt tcactggtga cggcattcga gacactcctt gtatccgcgt
     4381 tgtttgatat cttttaagat ttcccgggga ttaccatgac acttgatctc cccttcgcac
     4441 atcacgtgcc ccttgtcagc gtccagataa tcaaggatat aaccggtgtg cgttataaca
     4501 agcccgctct ttttgcgctt gataagatgc ttgtcttttt ccagcagtga accgatggca
     4561 gttccgatga gcgagatgtt ttccagatca accccgcttt ccggttcatc gagcatgata
     4621 aagtcggggt tctggatcat cagctgcaac acttcactgc gtttgatttc accgccggag
     4681 aatcccttat tgatgtcccg ctcaaggaat ttgtccatat gaactgattt ggccagctcg
     4741 ggtatgttat cttcactgtt cttggatatg gcggtcagca ttttcccaag cttcagccct
     4801 gagatggttg gtggacgctg gaagagcata ccgattcccc gttttgcacg ttcatgggct
     4861 ttcattgttg tcacatcttc ccctttgaag aggatttttc cctcggttat ctgatagttg
     4921 gtaaatccca ttacggtcat aagaagtgtt gttttacccg atccgttggg cccgagcagg
     4981 acatgggttt ccccctcccc tatgtggaga tttatatcgt gcaacacctc tttgtcgccg
     5041 atttttacat gcaggtcttc tatatgcagc atagattaat cgtagagaat atgtcgatta
     5101 ctgcattaaa cgcttttcaa ggtaccaaaa aaggggccct gtatattcct ttactaggat
     5161 atgaaataca aagcacgggt aagatgacgg aggggatagt gcctgtcccc ttttggtaat
     5221 tgtgatgatg acgtattacc tgttcaggaa ttatcgcatt tgggggagcc gaagcggcgg
     5281 ggtgaaaccc ccttgagcgt ttagcttgtt gttgagcatt tttacagaac ccaaaaaagt
     5341 gatactgttt cggatatttt aacacatcat ctggtaaagt acacacaggt atcatctcat
     5401 gagacctgaa aaggtacggg acaaagcagg taaggcgttt atccggaagg gttcgaaagg
     5461 tgcaaaaaag tccgttatat ccagtgcacc gcgtgagata ttaccttttg tcaaccatat
     5521 catatgtgcg gatgcactac acgcacttgc ctgtataccg gaccagagta tcgacctgat
     5581 catcacatct cccccctata atttcgggca tgcgtatgca cacgaccctc atgacgatac
     5641 tcacgagtgg aaccagtatt ttgaaaacct gctcggtata tggcaggagt gcgtacgggt
     5701 gataagaccc ggcggcagga ttgccgtgaa tatccagcca cttttttcag attatgtccc
     5761 tacccatcac cttatttctc accagcttgc tggcctgggt cttttatgga aagctgagat
     5821 tctgtgggag aaaaataatt ataatgcaaa gtatacagcc tgggggagct ggaaatcccc
     5881 gtcgatgcct tatataaaat acacctggga gttcattgaa gtttttgata aggaaaccca
     5941 caagaagtgc gggcggcagg aggatatcga tatcaccgct gaagaattca aggaatgggt
     6001 acttggacgg tggtcttttc caccggaaat ccatatgaaa gattacaacc atcctgcaat
     6061 gttccctgag gagttgccac gtcgtctgat gaaactgttt tcctacaaaa atgatatagt
     6121 gctggatccg tttaatggtg ccgggacaac gactctcgtc gcgtggaaac ttcaccgtcg
     6181 tttcatcggt attgatgtgt cggaccagta ctgcgaaacg gcattccagc ggcttgcttc
     6241 ggaagcggag aattgtgccc ccacttcacc tgaatatccc ctgccgtcaa tgatggttat
     6301 ggaacatgaa taacaggcca catcgtgccc tctctatgcc ggcatggtct tttttacata
     6361 ttcatataca cctttccggt catatatgtg ctcaagaata taattatgga taagagcata
     6421 tttttcatag agggtaaaga tctggcggta ggtatctaac cggctgttat actcctcgac
     6481 aagagcatta tggacaggta cctgtgcatt gtaacggccg gtatttctgg tagttttaaa
     6541 atcctgcatt tctgtttcga gccgggcaat tttttcctgt tgtgacgtaa tgtcttcact
     6601 catgcttttc acatgaggtt cgagttcctt aactttccgg ttagacagca catacatttc
     6661 gtgaatatag cgggtctcgc ttccactcct gtaggttttc gttccattgc tgatggggat
     6721 tataacagga tctgaataaa gggtgaggtt tgcccccagt gtatcagtcg gaaccccgac
     6781 aaaagagtaa ttggtcgttt ccaaaaatgc ataccctgtg tttttgtata tgtattcgtc
     6841 tgatccaact cctagtgcca tatgtgattc aggaccaaac aggagcaggg caacgggata
     6901 ccctgcccgt gacaacagcc cggctaatag caggctcttg tcatcacagt cccctgcacg
     6961 gtcaacaaca gtttctatcg gaaattttgc cgggttctgc tcacgggttt catacttgag
     7021 agactggata tatactgcaa tgagttcgag atattcatca tctgagagtt tttgccggag
     7081 ccggattttg tcagtttctg caagaagtgc ggcatagagg ctttcctgcg ccggatcctg
     7141 taccatcgca cggtaacttt gggcaagcca taccgtttct gagatgtttc catacactgt
     7201 tgtagctttt tccgcctgca ttgcaccttc atagaccgag acattgatgg gaattgtcag
     7261 gacaaccgtg gtattttcaa acgggaattg atgggaaata ttgagcggga aagtcttgag
     7321 actggataga tcaggagcaa tatggggcac aattatcgtt actttgggaa aaacaacagc
     7381 actgatgagt actgttgata ataccaggat aataatgagc aaaaaaatac ccgtgagttt
     7441 gaggaaccgg ttatgcatta ttcacctcac tatgcctgta ttgtttttct gacatattac
     7501 aaaatatccc gtagtcgtgc gatggaatca ataacataat ccggtttgat caccgcgttg
     7561 tccacagcat cttttcggta tttcccggtc cggacaagaa ctccctgcat acccatagta
     7621 cgtgccccgc caatatcggt aagaatatca tcgccaatca ttgccacctg gccggcacaa
     7681 agtcccatgt cgctgagggc aagtgcaaag aactttcttg agggtttacc aacaactgtt
     7741 gcttttttgc cggtcgcaaa ttcaagggcc tgaacaaagg gacccgcaga gagtgaaaga
     7801 ccgcccggtg ccatccagta ccggtctttc tccagcgcaa tcatatccgc gccattcatg
     7861 agatgacgga atgcggtgtt caggctgtca taagtaattt tttcaccggc atctcctata
     7921 ataacaacct cgatcccgct tacactgtcg catatacatt cgtgctcaaa gtcccggcaa
     7981 acatctcctg ttgtcagaag ataacaatga tgtttaccgg tatttttcat acaggcaact
     8041 gcagcaagtg gtggtgtgaa aatatgctcc acaggaatgt caaaccccag tgatgacaac
     8101 ttttcggcga gagttttccg gcatttacgg gttgtgttgg aaacaaacct gagcgtatat
     8161 ttcctgtccc tgaggagatc aatggtttcc cgggctcctt tgattgcgtg gtccccgaca
     8221 tacaaaaccc cgtcaagatc tatgagaaaa cccgtgacat ctatcatttt tttaagttcc
     8281 taacctgatg agccgccaaa tcccgttacg aaacagtttt catattctgt tgtatcaggc
     8341 actttccggt atcgcgggga tacgtttctt tcccggaaaa aatcggacga gatgtttccg
     8401 gttgaactgc atatctgtgg gcagggatat tggcgaggtt catggataag cagagttact
     8461 ccgctttaaa cattaaacgt cccttcaaac ctgtagaatg acctgaggaa aaacgaattc
     8521 ggtccagggg tattgatggt gtatcaagga ccctgccatg taaatttacc cggcaagtca
     8581 gctttacttt cacccccctt ggcggggtgt ttaaatccag ggcgccgttt ttgtattcag
     8641 acgatgtcgg taacacaagc ccctgcaagg attggtgccc tctggcagca gcgcataaaa
     8701 acgatgcg
//
LOCUS       DASRLV010000116         5893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_122499,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000116 DASRLV010000000
VERSION     DASRLV010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5893
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122499"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1442
                     /locus_tag="VFC43_04685"
     CDS             <1..1442
                     /locus_tag="VFC43_04685"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HZK30514.1"
                     /translation="LACARIGAVHSVVFAGFGPAALNMRIQDASAKVVITADSSVRRG
                     KTIPLKSIVEEALINAPSVEHVIVLRRQDPPVDLRPGLELDFNEIMNSAPQDCPAEVM
                     DAEDPLFTLYTSGSTGKPKGIVHTCAGYMVGTYYTSKYIFDMKDPDIFWCTADPGWIT
                     GHSYIVYGPLAIGATVFISELTPDYPDAGSYWSLIEEQKITIFYTAPTAIRMFMKMGE
                     QWPDKYNLSSLRIIGSVGEPLNPEAFEWYYHVIGKDKVPILDTWWQTETGMHMITTMI
                     GEPMRPGFAGKPIPGVEADVVDKDGKSVKPGTGGLLVIKSPWPSMLRMVWKDDERYRK
                     YWETIPGVYTVGDLAVKGKDGYIMILGRSDDIIVVAGHNIGTAEVESSLVSHHAVAES
                     AVIGKPDAMKGNSIKAFVILRLGNSPSDKLKNDLVYHVRMTLGPIAVPQEIEFVDKLP
                     KTRSGKIMRRVLKAKEMGLDPGDISTLEE"
     gene            1468..2733
                     /locus_tag="VFC43_04690"
     CDS             1468..2733
                     /locus_tag="VFC43_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HZK30515.1"
                     /translation="MGSTSQGKLFGMEPEKGRWILVILGLIINLCLGSIYSWSVFVGP
                     LTNYFTKDLGQTVSASEILLPFSVFLAFFAIAMPFTGKYIEKYGPKNITIVGGCLTGL
                     GWLLSSFAGSVQWLYLFYGVIAGIGVGIAYGVPVAVAARWFPDRRGLAVGFTLLGFGF
                     SAVLTANIAGFLIGTTGVMNTFRIFGIAFVLLIVVLSLPLKFPVTGWTPAGWTPPVQA
                     AGNAVTCEFRREQMLKTPAFYALWACYFIGCLAGLMAIGIAKPVGVDVGVETGLATML
                     VGAFAVFNGFGRPVFGTLTDKLTPRNTALVSFILIALASVLIWQIPTVPVYILAFAIL
                     WGCLGGWLAIAPTATASYFGTCDYPRCYGVLFLAYGAGGIAGPQLAGFIKTSTGSYMG
                     VFPYVLVLAAIGLLIAFILLKPPQAPQKN"
     gene            complement(2730..3848)
                     /locus_tag="VFC43_04695"
     CDS             complement(2730..3848)
                     /locus_tag="VFC43_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-NAD kinase family protein"
                     /protein_id="HZK30516.1"
                     /translation="MKRIGFLINPVAGMGGAVGLKGTDGNVAEARRRGALPHAKNRAR
                     GTLELLAQDNSLEFVTCSGAMGEEALNEARIGAVKVIYVFNGESSARDTIRAAQIFQK
                     TGVDLILFCGGDGTARDIFDAVGKDVPILGIPAGVKMYSAVFAIDPPTAAELVKRFDM
                     KSLRDSEVMDVDEDAYRAGELKTRLHGIARTPSLAGKVQLSKQVFEEGDEERAKREIA
                     RFIREIMIPDVIYILGAGTTTESIANELGMEKTLLGIDAVRNGQIVARDIDEKTLWTM
                     LNEFDELKILLSPIGAQGFILGRGNQQISPRIVRKVGIRNIIVVATPHKLRETPVLYV
                     DSGDSELDLEFGDSLQVVSGYRMAQRKRIYQLRPDKKD"
     gene            complement(3863..5221)
                     /locus_tag="VFC43_04700"
     CDS             complement(3863..5221)
                     /locus_tag="VFC43_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HZK30517.1"
                     /translation="MQTKILLDEEQMPKKWYNVQADLKSPLDPPLHPKTQKPIGPEDL
                     AAIFPMELIRQEVTTDRHIDIPEDVQDVLRLWRPSPLYRAHRLEKFLKTPARIYYKWE
                     GVSPAGSHKPNTSIPQAYYNMKAGVERIATETGAGQWGSALAFATMLYDLECTIYMVR
                     SSYTQKPYRKSMMHVWGAECIPSPSTKTNAGRAMLEKDPDTPGSLGMAISEAVEDAAS
                     HSNTNYALGSVLNHVCLHQTVIGLECREQLAMVDDYPDVVIGCVGGGSNFAGISFPFA
                     GDKLSGKHKDVDIIGAEPASCPTLTKGLYTYDLADEAGYTPLLKMFTLGHDFVPPTMH
                     AGGLRYHGDSPIVSRLVHDGIMRAVAYHQSEVFDAAQTFARTEGIIVAPETSHAVKSA
                     IDEAMKCKNTGEAKTILFNCSGHGNFDMTAYDAYYAGQLVDYEYPDELIRESLARIPK
                     IS"
     gene            5504..>5893
                     /locus_tag="VFC43_04705"
     CDS             5504..>5893
                     /locus_tag="VFC43_04705"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HZK30518.1"
                     /translation="MKHSIVDILFRPGVFFQHTILEKENLKIPALIVLVLGIVSAFYA
                     YLVGGVTGKMMAGLFSGMESIIAISSVIGAIIGVFIFWAIWTGVFYLISSVFKGNGTF
                     KRTLEFTGYGYLPQVFGAILMVIVAIQY"
BASE COUNT         1405 a         1445 c         1444 g         1599 t
ORIGIN      
        1 tgcttgcctg tgcccgtatc ggtgcggtgc acagcgtagt ttttgcgggt tttggtcccg
       61 cagcccttaa catgcgtatc caggatgcct ctgccaaggt tgtcataact gctgacagca
      121 gcgttcggcg cggcaaaacc ataccgttga aatcaattgt ggaggaggca cttatcaatg
      181 ccccatcagt cgaacatgtc atcgtgctca ggcgacagga ccccccggtc gatttaaggc
      241 ccggtctcga acttgatttc aacgaaataa tgaatagtgc accacaggat tgcccggctg
      301 aagtcatgga tgcagaagac ccgcttttta ccctttacac cagcggatcc acgggtaagc
      361 ccaaagggat tgttcacacc tgtgccggat atatggttgg aacctattac accagcaaat
      421 atatcttcga tatgaaagac cccgatattt tctggtgcac ggcggatcct ggctggatca
      481 ccggccacag ttatattgta tacggccccc ttgccatcgg agcaacggtg tttatttctg
      541 agcttacgcc ggattatccc gatgcaggaa gctactggag cctgatcgaa gagcagaaga
      601 tcaccatttt ttataccgca ccaactgcaa tccggatgtt catgaagatg ggtgagcagt
      661 ggccggataa gtacaattta agttccctgc ggataatcgg atccgtcgga gaaccactca
      721 accccgaggc tttcgagtgg tactaccatg ttatcggaaa ggacaaggta ccgattctcg
      781 acacatggtg gcagacagaa accggtatgc acatgatcac cacgatgatt ggggaaccaa
      841 tgcgcccggg ctttgccggc aaacctattc ccggtgtgga agcagatgtg gttgataaag
      901 acggaaaatc ggtcaaaccg ggaaccgggg gacttcttgt catcaaatct ccctggccat
      961 ccatgctcag gatggtctgg aaggatgacg aacggtaccg aaagtattgg gagacaattc
     1021 ctggtgtcta cactgtaggc gatcttgcgg ttaaaggtaa ggacggttat atcatgatcc
     1081 ttggccggtc cgatgatatc atcgtcgttg ctggtcataa tatcggcaca gcagaagtgg
     1141 agagctcact cgtctcccat catgcagttg cggaatcagc agttataggg aaacctgatg
     1201 ccatgaaggg taattccatc aaagcattcg taattctccg tttaggcaat tccccgagtg
     1261 acaaactcaa aaacgacctg gtatatcacg tgcgtatgac actggggcca atagcagttc
     1321 cccaggagat cgagttcgtc gacaaactcc ccaaaacgag gagtgggaaa atcatgcgca
     1381 gggtcctgaa agcaaaagaa atgggtttgg acccgggtga tatctctaca ctcgaagaat
     1441 aaatacaaat ttttgagggt ggcaacaatg ggatcaacat cacagggaaa attattcggg
     1501 atggagccgg aaaaagggcg ctggattctc gtaatactgg gcttgatcat caacctgtgc
     1561 ttaggctcga tttattcatg gagtgtgttt gtcggaccgc tgacgaacta cttcaccaag
     1621 gatctgggtc agactgtatc cgcaagtgag atcctgttac cgttttcagt ctttctggca
     1681 ttctttgcca tcgcaatgcc atttaccggt aaatatattg aaaaatatgg tccaaagaac
     1741 ataaccatcg tgggtgggtg tcttaccggg ctgggctggc tcctctcgtc gtttgccggc
     1801 tctgtccaat ggctatacct cttctacggt gtgattgccg gtattggcgt tggcatagca
     1861 tatggtgtgc cggttgcagt tgcagcccgg tggttcccgg atcgccgggg gcttgcagta
     1921 ggatttaccc tgcttggatt tggtttttct gcggtcctca ctgcgaatat cgcaggtttc
     1981 ctcattggaa cgaccggggt catgaatacc ttcaggatat ttggtattgc atttgtactc
     2041 ctgatcgtcg tactctcact ccccctaaaa tttcctgtta ccggatggac tcctgcagga
     2101 tggactccgc cagtccaggc agccggcaat gcggttacct gcgaattcag acgtgagcag
     2161 atgctcaaaa cgcctgcctt ctacgcactc tgggcatgtt acttcatcgg gtgtcttgcc
     2221 gggctcatgg caattggtat cgcaaaaccc gttggagtag atgtcggtgt tgagacaggg
     2281 cttgcaacaa tgctcgttgg tgcttttgcg gtattcaacg gttttgggcg cccggtcttt
     2341 ggcaccctta cagacaagct gacaccaagg aatactgcac ttgtatcctt catactgatc
     2401 gctcttgcat ccgtgctaat ctggcagatc ccgactgttc cggtatacat ccttgccttc
     2461 gccatcctct ggggatgcct gggcggctgg ttggccattg cacctactgc gacggcaagt
     2521 tatttcggca cctgcgacta cccacgatgt tacggcgtat tattccttgc atacggtgct
     2581 ggcggtattg ccgggccgca attagccggg ttcataaaaa catcaacagg gtcatatatg
     2641 ggggtctttc cctatgtcct tgtccttgca gccataggct tgctgattgc atttattctc
     2701 ttaaaaccgc cccaggctcc tcagaaaaac taatcctttt tatcgggacg gagctggtag
     2761 atgcgcttcc tctgggccat ccggtaccct gatactacct gcaaagagtc gccaaattct
     2821 aaatcgagct cggaatcgcc ggaatctaca tagagaaccg gtgtttcccg gagtttgtga
     2881 ggagtggcga ccacaataat gttacggatt cccacttttc ggacgatgcg gggactgatc
     2941 tgctggtttc cccttccaag gataaaaccc tgggcaccta ttggactgag tagtattttt
     3001 agctcatcaa actcattcag cattgtccat aatgtttttt cgtctatatc cctggcaaca
     3061 atctgaccgt tcctcactgc atcaattcca agaagggttt tttccatgcc aagttcattg
     3121 gcaatcgatt ccgtagtcgt tcctgcacca aggatataga tcacatccgg tatcataatc
     3181 tcccggataa atctcgcgat ttccctcttt gcacgttcct cgtcaccttc ttcaaagacc
     3241 tgcttggata gctggacttt tcctgcaagc gatggagtac gggcaattcc gtgtaaacgg
     3301 gttttcagct ctcccgcccg gtacgcatcc tcatccacgt ccatcacttc tgaatcacga
     3361 agtgatttca tatcgaatcg tttcacgagt tctgcagctg ttggaggatc gatggcaaag
     3421 acagccgaat acatctttac tccggcgggt atcccaagta tcggtacatc ttttcctaca
     3481 gcatcaaaaa tgtcccgggc tgttccatcc ccgccacaaa aaagaatcag gtcaacaccg
     3541 gttttctgaa atatttgtgc tgcccggata gtatcccggg cgctgctttc gccattaaaa
     3601 acatagatta ccttcaccgc gcctattctg gcctcattga gggcttcttc tcccatggca
     3661 ccagaacagg taacaaattc cagactatta tcctgtgcga gaagttcaag ggtgccgcga
     3721 gctctgtttt ttgcgtgggg taaagccccc cggcgacgtg cttctgctac attgccatcg
     3781 gtccccttga ggcccactgc ccctcccatc cctgccacgg ggttgatgag gaaacctatt
     3841 cgtttcatga aaaaaagtga tttcaggaaa tttttggtat ccgggcaagt gactctctga
     3901 tcagctcatc cgggtattca taatcgacca gctgacctgc ataatatgca tcatacgctg
     3961 tcatatcaaa attgccatgg ccggagcagt tgaacaggat tgtctttgct tctcctgtat
     4021 ttttacattt cattgcttca tcaatcgctg attttaccgc atgagaagtc tctggggcga
     4081 caataatccc ttcagttcgg gcaaaggtct gggctgcatc aaagacttca ctctggtggt
     4141 aagcgactgc tctcattatc ccatcatgga caagacggga aacaatcggt gaatcgccat
     4201 ggtaacgaag acctcccgca tgcatggtgg gagggacaaa atcatgcccg agcgtaaaca
     4261 tcttgaggag cggggtgtaa cctgcttcat cggcgagatc gtaagtgtac aatccttttg
     4321 taagtgtagg gcacgatgcc ggttcggcac ctattatgtc cacatcttta tgcttcccgg
     4381 aaagtttgtc acctgcaaac ggaaatgaaa tgcctgcgaa gttggatcct ccgccaacgc
     4441 acccgattac aacatccggg tagtcatcca ccattgccag ctgctcgcgg cattcgagtc
     4501 ctatcactgt ctggtgaagg cagacatggt ttaagaccga gcccagggca taattggtat
     4561 tgctgtgaga tgctgcatct tctacagctt cagaaatggc cattccgaga ctgcccggag
     4621 tatcaggatc tttttcgagc attgcacggc cggcatttgt tttggtgctc gggctgggaa
     4681 tgcattctgc tccccagaca tgcatcatgg atttccggta gggtttctgc gtgtaacttg
     4741 accggaccat gtagattgtg cactcaaggt catagagcat ggtggcaaaa gcgagtgccg
     4801 atccccactg cccggcaccg gtttcggtag cgatcctttc tactccggcc ttcatgttat
     4861 agtacgcctg cggtatcgaa gtatttggtt tatgactccc tgcagggctg actccctccc
     4921 atttataata aatcctggct ggtgttttta agaatttttc aaggcggtgg gcacgataga
     4981 gggggcttgg cctccagagc cgtagcacat cctgcacatc ctcgggaata tctatatgcc
     5041 gatcggtggt cacttcctgt cgtatcagct ccatcggaaa aatggcagcg aggtcctcag
     5101 gaccaatagg tttctgggtc ttggggtgga gcggaggatc aaggggtgat ttcaggtcag
     5161 cctggacatt gtaccacttt tttggcatct gctcctcatc cagaaggatt tttgtctgca
     5221 tacatagtca ttgcgttgta taaatatata cattattgta cacatttgat tttttcggat
     5281 gtttagtata catcggaaat taaatgacag aatcaatcag gtatgaacat tcgtatcgaa
     5341 tttattctag tctcaaaaac tacacctgct atcgaatccg ggaacaacct attttgacat
     5401 tcgttccaaa ttattaatag cataaagaga aaatgagtta tcaagttgtt atttctgcaa
     5461 caacaaacca gatccatgga aaaagggaaa aggggatgat cagatgaaac attcaatagt
     5521 tgatatttta ttcagacctg gggtgttctt ccagcacacc atcctggaga aagaaaatct
     5581 caaaattccc gcacttatcg tattagtgct ggggatagtc tccgcatttt atgcttattt
     5641 ggtcggcgga gtaacgggaa agatgatggc gggattgttt tccggtatgg aatccataat
     5701 cgccatttct tctgttattg gtgccataat cggagtcttc atcttctggg cgatatggac
     5761 gggagttttc tacctgatat cctcagtttt caaaggtaat gggacattta aaagaactct
     5821 tgaatttacg ggctatgggt atcttcccca ggttttcggc gcgattctta tggtgatcgt
     5881 tgcaatccag tat
//
LOCUS       DASRLV010000117         6429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_848893,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000117 DASRLV010000000
VERSION     DASRLV010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 6429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6429
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848893"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /gene="hisE"
                     /locus_tag="VFC43_04710"
     CDS             <1..246
                     /gene="hisE"
                     /locus_tag="VFC43_04710"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HZK30519.1"
                     /translation="RAITPSQESYTSRLLTDIKGIDKVLEKIGEESAEFIIAAKNGKP
                     ERTVEEASDLIFHLLVALRAADIDLSDVMQELKKRRK"
     gene            complement(543..995)
                     /locus_tag="VFC43_04715"
     CDS             complement(543..995)
                     /locus_tag="VFC43_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HZK30520.1"
                     /translation="MTQVRCEVKGVFVAMSDSTTVPLVVLSDGGELSLPIFIGLWEAV
                     SINSAKNKEILSRPFTHDLFLDLAAKFSITFHSLQIDSIEDGVYYAQLVLSADNHKEF
                     IDCRPSDGIALALRADVPIFVDGNVLSSTGQKTSDLPPMVYLSTFLRK"
     gene            complement(1057..2076)
                     /locus_tag="VFC43_04720"
     CDS             complement(1057..2076)
                     /locus_tag="VFC43_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HZK30521.1"
                     /translation="MEKSQVKWLYISIGFSLLVLVIILYFTINENTVHYLQQINPVFL
                     FLAFCTHILTMCFWAWRIQKMSGSLGYCVGFFYSLNLVFANLLAAAITPAQAGGEPVR
                     IHELYRANVPIGDATAIVIMERALDGIALAALAAFAMIVLTDQWKSLGAISEIMVYIT
                     WIFVAGSLFLFYLAVKRPDVVKRVVNRIACFFTRTWESARVEKFVLRADKEIDNFSVT
                     VIKFVKSAKGGLLWGMLFTMLYWVSEILTASLILVGLGQPPLILESFVIQLILAILMM
                     LPLTPGSSGIAEAGAYSMYALFIPVSIVGIFVVIWRIVLYYFNIALGILSSIIIVRRE
                     AQCKE"
     gene            complement(2217..2792)
                     /locus_tag="VFC43_04725"
     CDS             complement(2217..2792)
                     /locus_tag="VFC43_04725"
                     /inference="COORDINATES: protein motif:HMM:NF015943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF357 domain-containing protein"
                     /protein_id="HZK30522.1"
                     /translation="MKITECQILFTSSLSTTRISALEGTHYNKIAASVIQMACAYESD
                     GIRFFESCDPVNALASYYYGFGWLHFGLSSGLLLVTCPSVCPFKEPHEMLQLPFRQKL
                     EEKASRYGQLLEIARKSVKYAPDPATTSHDFAQRILCIAGVYARRGVCLERSGNHEDA
                     LASFSYGHGWLDAGVTSGFFHILAERDLFTV"
     gene            complement(2789..3538)
                     /gene="dph5"
                     /locus_tag="VFC43_04730"
     CDS             complement(2789..3538)
                     /gene="dph5"
                     /locus_tag="VFC43_04730"
                     /EC_number="2.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004164 - diphthine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0017183 - protein histidyl modification to
                     diphthamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine synthase"
                     /protein_id="HZK30523.1"
                     /translation="MLSFIGLGLYDKMDVSEKGLTTIRNADHIFLENYTSRLMGTSYE
                     DLQTHYGKPVQLLGREDVEQHPDEILSKAEYEQVVFLCAGDPMVSTTHADLRMRAASR
                     GIKTVIIHAASISSAVCGLSGLQNYRFGKSCSLPFPQKNWFPTSSIDVISQNLSLRLH
                     TLVYLDIQDGRYMLVPEAVTLLEKIAAEKKMHIALYVGIARAGSDNPLVQAGSAEVLR
                     SIDFGPPLHILIVPAELHDMEKSYLEMFAGL"
     gene            complement(3693..5543)
                     /gene="feoB"
                     /locus_tag="VFC43_04735"
     CDS             complement(3693..5543)
                     /gene="feoB"
                     /locus_tag="VFC43_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HZK30524.1"
                     /translation="MKIALLGNPGAGKSLIFNQLTGLGIEVNRYPGSSVCLECGNVCY
                     KKEKIEIVDLPGIYSLDGSSDEEMLVRKFLEQAGADVIVAVLDATKLERSLYLLTQVT
                     EFSPKLIVVVNMIDEAEHQGLIIDIDKISSCLGVPVILTAASEGRDIDRILPLALGSG
                     RVATLQVPYDHHIEAALKSLEKTLGTPRHKSLLALQGTGEDRMLLDAAGAVAHEIELR
                     HRMSVRQIIATNRHNLSRKIAASAVRQGDRKKTYDLDSLLTRTFPGVPILVAILFFLL
                     LSVFVIGSWMEGIIVESFTRSVIIPFTALALPPLWDRIGFSMLIALQAGFGIAFPFIF
                     TFYLGLSLLEDTGYMTRAAFLADRAMHRLGLHGEAIIPMVIGFGCTVPAVMSLRILKT
                     RREQTIAAFLISMVPCSARTVVIAGIVAAFVGILWAFSIYIIVFILIIFTALILSRIT
                     PGEQFGMIVEMSALRRPQAHLVLGKTWLRIKEFLFIAMPILIITSIGLGLFQYFGLTD
                     AFQQMVAPITVTLLGLPEYASTALLFGIFRKELAFETLAVLAGTADLGSVMTPAQLYG
                     FAIVSVLFIPCTSTIAVLYRQLGARITALISIYTVFLGLLIGAVINLLMK"
     gene            complement(5540..6214)
                     /locus_tag="VFC43_04740"
     CDS             complement(5540..6214)
                     /locus_tag="VFC43_04740"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HZK30525.1"
                     /translation="MQLSLREDYLEAIQHFSEKNARSPHYGELAAALGTSEPVVRSDM
                     EDLVSSGDVSILSGGVISLTGRGQQTGLSVIKKHETLQCFLSEILGMDSSSASDEACT
                     LEHSVSDETIDRLGKLIVNHDGTLPRGRGRNGRQDSTAGCISAEGECQYPSISDCAEG
                     ENVIIHCIHGRASAKRLLDLGMVPGERASIKRKLSNGALVVQVKGCDVALSPEIACAI
                     GVARSK"
BASE COUNT         1792 a         1534 c         1549 g         1554 t
ORIGIN      
        1 cgggcaataa ctccctcgca ggaatcctac acaagccggc tgcttactga tattaagggt
       61 atagataagg tgcttgaaaa aataggcgag gagtcggcag aatttattat tgctgcaaaa
      121 aacggtaaac ccgaacgaac agtcgaggag gcatctgatc tcatctttca ccttcttgtt
      181 gcattgcgtg cagccgatat tgatctctcc gatgtgatgc aggaactcaa aaaacggcga
      241 aaataggaac tgtgggaacg gatatgaatc gattcggttc agtccggaac catgcaaatc
      301 gtttttttcg atggattccc gcatcagacc atttcagatg cacggcaggc cgccgttttt
      361 aaatttttgg ccatcgatcc acagcgggaa tgttccttcg ggagcggcgg cattcatcgt
      421 accagatgga aatagtagta acaataccta tcatatgtga ataccacgat tttctcaagg
      481 aaacgaattt gaatgaattc ggaatatttc tgtttcacca gagccgaaaa tacccatcag
      541 cattatttcc gcagaaatgt ggacaggtaa accatgggtg ggaggtccga agttttctga
      601 ccggttgagg aaagtacatt cccatcaacg aagatgggga catccgcacg gagtgcaagt
      661 gcaatcccgt cactgggccg gcagtcaata aattccttat gattatcagc agagagcacg
      721 agctgggcat aatagacccc gtcttcaatg ctatctattt gcagagaatg aaaggtgata
      781 gaaaattttg cagccagatc caaaaaaaga tcgtgcgtaa aaggtctgga taaaatttcc
      841 ttgtttttcg cgctattgat ggaaactgcc tcccagagcc cgataaatat tggcaggctt
      901 agttctcctc catctgaaag tactacaagg ggcacagtag tagaatcact catggcgaca
      961 aagactcctt tcacctcaca cctgacttga gtcatggttt ttcatattga ttgctgttac
     1021 tatcagtgat aagagttaag gtaagtaagc tcggattcat tccttgcatt gtgcttcacg
     1081 acgaacgatt atgatgcttg agaggatacc gagtgcaata ttgaagtagt agaggacaat
     1141 cctccagata acaacgaata tacccacgat tgatacgggg atgaacagtg catacatcga
     1201 ataggcacct gcttcagcaa tacctgagct tccaggagta agcgggagca tcatcaggat
     1261 tgcaaggatg agctgaatga caaatgactc aagaataagg ggtggctgcc cgagtccgac
     1321 aaggatgagc gaagcggtaa gaatctctga tacccagtag agcatggtaa acaacatccc
     1381 ccataacagc cccccttttg cacttttaac aaatttgatg actgtgacac tgaaattatc
     1441 aatttccttg tcagcccgca ggacaaattt ttcaactctt gcactttccc atgtacgggt
     1501 gaagaagcat gcaatacggt ttacaaccct ttttacaaca tcaggccgct ttactgcaag
     1561 gtaaaagaga aacagggatc ctgctacaaa gatccaggtg atatacacca tgatttccga
     1621 tatggctcca agacttttcc actggtcagt caggacaatc atcgcgaagg ctgcaagcgc
     1681 agcaagcgcg ataccatcta acgccctttc catgatcact attgctgttg catccccgat
     1741 aggcacgttc gcccggtaga gctcatgaat acggaccggc tccccccctg cctgagcggg
     1801 ggttatcgcg gctgcaagaa ggttggcaaa aacaaggtta aggctataaa aaaaacctac
     1861 acagtacccc agtgagcccg acattttctg tattcgccat gcccagaagc acatggtgag
     1921 gatgtgggta cagaaagcaa ggaaaaggaa gacggggttg atctgttgga ggtaatggac
     1981 cgtgttttca ttgatggtga agtacaggat tatgactaaa acgagaaggg agaacccgat
     2041 tgaaatatag agccatttta cctgagactt ctccatgtga gttccccgtc aaaataagca
     2101 cagattatga agtgcgccgt atcttacaca ttattgaaca tagtataaac gcttttttga
     2161 catgactccg tttcaggata aaagaacctt atcaaaatga tgttctgttc ttacgattat
     2221 acggtgaaaa gatcccgctc tgccaatata tggaagaatc cggaagtaac gccggcatcg
     2281 agccagccat gcccgtaact gaaactggcg agggcatcct catgattacc cgatctctcg
     2341 agacaaacgc cgcgtctggc ataaacccct gcaatacaaa gaattcgctg tgcaaaatca
     2401 tggctggttg tcgccggatc cggggcatat ttcacggatt ttctggcaat ttccagcagt
     2461 tgtccatagc gggatgcctt ctcttcaagt ttctgcctga aaggaagttg aagcatctca
     2521 tgcggttcct taaatgggca tacgctggga caggtgacta aaagcagtcc tgatgacaaa
     2581 ccgaaatgca gccagccgaa cccgtagtaa taacttgcga gtgcgtttac gggatcgcac
     2641 gattcaaaaa acctgatccc gtcgctttca taagcacatg ccatctgaat gacagaagca
     2701 gcgattttgt tgtaatgggt gccctccagt gcggatatcc gtgtagttga aagcgatgaa
     2761 gtgaaaagga tctggcattc tgtaatcttc ataagccggc aaacatctcc aggtacgact
     2821 tctccatgtc atgaagttct gccgggacta tcaggatatg aagtggtgga ccgaaatcaa
     2881 tagatcgcaa tacctcagca gatcccgcct gaaccagggg attgtcggat cctgcccgtg
     2941 caatgccaac atacagggct atgtgcatct ttttttcagc tgcaatcttt tcgagaaggg
     3001 ttacagcctc cggtacaagc atatatcttc catcctggat atcgagatac accagcgtat
     3061 gcaaccgcag tgacaggttc tgcgagatga cgtcaatgga tgatgtcggg aaccagtttt
     3121 tctgcggaaa aggaagcgaa caggactttc caaaccggta attctggagc ccggaaagac
     3181 cgcaaaccgc acttgagatg gatgcggcat gaataattac ggttttgatc cctctcgatg
     3241 cagcccgcat ccgaaggtct gcatgggtag tggagaccat gggatcaccg gcacagagaa
     3301 aaactacctg ttcatactct gctttgctca atatctcatc gggatgctgt tcaacatctt
     3361 cgcggccgag cagttgtaca ggtttcccat aatgtgtctg caggtcttca tacgacgtgc
     3421 ccatgagcct ggaggtgtaa ttttcaagaa agatgtgatc cgcattgcgg atagtagtga
     3481 gccctttttc tgaaacatcc atcttgtcat agagtccgag gcctataaat gacagcaacg
     3541 tttccctcca cgttcagcag agaagctgtt gattctgcat atcccgcagc acaggtctat
     3601 aagaataatt atcgttcttc ctcccttcca aaaccacggt acatctgata actctgtgca
     3661 atcccctttt cttccattca agtgatgatt gatcatttca taagcaggtt tataaccgct
     3721 cctattaata agcccagaaa taccgtgtat atcgatatca gggcagtaat ccgtgcccca
     3781 agctgccggt acagaaccgc aatcgtggaa gtgcagggaa taaaaagcac actcacgatt
     3841 gcaaatccgt agagctgggc aggtgtcata accgaaccaa ggtcagcagt accggcaagg
     3901 actgcaaggg tttcaaaagc gagttctttt ctgaagattc caaataacag agcagtggat
     3961 gcatactccg gcaacccaag cagggttacc gttatgggag caaccatctg ctggaatgca
     4021 tcggtcagcc cgaaatactg gaaaagtccg agtcctatgc tggtgatgat caaaattggc
     4081 atcgcaataa acaggaactc tttgatccgg agccatgttt ttcccagtac caaatgcgcc
     4141 tggggtctgc ggagcgcaga catctcaaca atcatgccaa actgctcacc gggtgtgatg
     4201 cggctgagta tgagtgcggt gaaaataatc aggatgaaca cgatgatata gatggaaaac
     4261 gcccagagaa tcccgacaaa agcagcaaca atgcccgcta tgacaacggt cctggcagaa
     4321 caggggacca tgctgataag aaacgcggct attgtctgtt cccggcgtgt tttaagtata
     4381 cgcaggctca tcacggctgg taccgtgcat ccaaacccta tcaccatggg gatgatcgcc
     4441 tctccgtgca ggccgagccg gtgcattgcc cggtccgcaa ggaatgcggc acgggtcata
     4501 tatcccgtat cctcaagcag cgagagtccc agataaaacg tgaagataaa cgggaatgcg
     4561 atcccaaaac ctgcctgaag tgcaatcagc attgaaaagc cgatacggtc ccacagtggt
     4621 ggcaatgcga gggcagtaaa cggtatgatc acagatcgtg tgaatgattc aacaatgatc
     4681 ccttccatcc aggagccgat gacaaagact gagaggagaa gaaaaaatag gatggcgaca
     4741 aggatcggga ctccgggaaa tgtccttgtg aggagactgt ccagatcata ggtttttttc
     4801 cggtctccct gcctgacagc ggaagctgca atcttccggg acaaattatg ccggttcgtt
     4861 gcaatgatct ggcggaccga catcctgtgg cgcagttcga tctcatgagc gacagctcct
     4921 gcagcatcaa ggagcatgcg gtcctcgcca gtaccctgca gggcaagcag gctcttgtgg
     4981 cggggtgtcc caagagtttt ttcgaggctt ttaagtgccg cctcgatatg atggtcgtag
     5041 ggcacctgaa gggtcgcaac tctgccggaa cccagtgcga gaggaagaat gcggtcaata
     5101 tctcttcctt cagacgcagc cgtaagaatg acggggactc ccagacaaga tgaaatcttg
     5161 tcaatatcga tgatcagccc ctgatgttcg gcttcgtcaa tcatattgac tacgacaatg
     5221 agttttggtg agaattcggt gacctgggtt aaaagataaa gacttcgctc aagttttgtg
     5281 gcatcaagga ctgcgacaat gacatctgct cctgcctgtt caaggaattt acgcaccagc
     5341 atctcttcat ccgaactccc gtcaagagaa tagatcccgg gcaagtcgac aatctcgatc
     5401 ttctcttttt tataacagac attgccgcat tccaggcaga cagaggatcc cggatagcgg
     5461 ttcacctcaa taccaagtcc ggtgagctgg ttgaaaatga gggacttccc tgcgccggga
     5521 ttacccagga gtgcgatctt cattttgatc gcgccactcc tatcgcacat gcgatctcgg
     5581 ggctcagggc aacatcgcaa cctttgacct gtacaacaag tgcaccattg gataattttc
     5641 gttttatgga agctcgctcc ccgggtacca taccaagatc aaggaggcgt tttgcagatg
     5701 ccctgccatg gatacagtgg atgatgacat tttctccctc agcacaatca gaaatactgg
     5761 gatattgaca ctctccctct gcagatatgc accctgcggt actatcctgc ctcccgtttc
     5821 tcccgcgccc gcggggcagc gtaccatcat gattcactat caacttgcct aaccggtcaa
     5881 tggtttcatc cgagacagag tgttcgagcg tgcatgcctc atcggacgca gagcttgaat
     5941 ccatgccaag aatctctgat aaaaagcact ggagtgtctc atgttttttt atgacactta
     6001 agccggtttg ctgtccccgt cctgtcagcg aaattacacc gccggataat atggagacat
     6061 ccccactgga aacaaggtct tccatatcag aacgcaccac gggctctgaa gtacccagtg
     6121 ctgcggccag ctctccgtag tgaggggaac gagcattttt ttccgagaaa tgctggatgg
     6181 cttccaggta atcttcccgt aaggagagct gcatagagta ctctttaact atttggggtt
     6241 cctgacaact ttattgattt gggttaaaaa ttcacaggac cggaaataat gtgcactact
     6301 ataaactata aacaaatgaa aagataaaaa atataaaata atttaaaggg tgtttttggg
     6361 agttctgatg atttactggc tgtatgaata cctgctcaaa cggcagatca ccgagttgcc
     6421 gggtcatgt
//
LOCUS       DASRLV010000118         4321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_527387,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000118 DASRLV010000000
VERSION     DASRLV010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527387"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VFC43_04745"
     CDS             <1..311
                     /locus_tag="VFC43_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="tungstate ABC transporter substrate-binding
                     protein WtpA"
                     /protein_id="HZK30526.1"
                     /translation="LKYITLPADIDLSDPKMEAAYKTMQVKRPSGNTTVTEVGIPIVY
                     AATVPASARTPDSGYEFINLLLSSDGQAVLNTDGQTPIVPAEAYGTVPGAILQNVKKV
                     "
     gene            complement(491..1612)
                     /locus_tag="VFC43_04750"
     CDS             complement(491..1612)
                     /locus_tag="VFC43_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HZK30527.1"
                     /translation="MSPKPAIPMNFSLSELAGSLGDFGTIIPLIIAVALVSDVNTRYV
                     LLFFGIWFILTGLYYRLPIPLEPMKAVAVIVIAGGISRGEIAASGLILGVIFLVLGYG
                     RVFEVIDRWVPQSVVRGIQLGLALLLLKASAGFVIKDPFFFILGIGIVIAFLLLFRYR
                     RVPDLSSLCVIGVGLIGGLLLNGIPPLSLIPAPQLVIPLPADFSSAFSTLVLPQVVLT
                     IANAILATSLLTKDLFSADVPPKKLSTTIGLMNLTSVPFGGFPMCHGAGGLAGQYRYG
                     ARTGGANVYAGLIFIILALFFTSPQILSIIAVGVLGALLVFVGIEMARHSLRTDSLAV
                     TCIIGILALVSSMTIAFILGMVIASLLRWVGRRKVQKTC"
     gene            1822..2550
                     /locus_tag="VFC43_04755"
     CDS             1822..2550
                     /locus_tag="VFC43_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK30528.1"
                     /translation="MSEIVSGFFQAINLIVTLNPEVMQIAALSLFISLAATIFAAVIS
                     IPLGGLIYFHEFRGKRVLIVLIQTLYSVPTVVVGLILYLFLSRDGPLGIFGLLFTPEG
                     MILGQTLLIIPIMTGLVIAALGGVDRNISDTLISLGATGFQAIIHIVREARLAILSAV
                     VLGFGRAISEVGVAMMIGGNIRGATRVLTTAIALQTGIGDFPFSIALGMILLLIALVV
                     VIILNLITSGLSFESQVALGGPRT"
     gene            2547..3632
                     /locus_tag="VFC43_04760"
     CDS             2547..3632
                     /locus_tag="VFC43_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK30529.1"
                     /translation="MIRIADLSRRAGSKTILEPINLEIHRGEIFTIIGPSGSGKTTLL
                     RLIDLLDMPTTGTIVFDGTDTAASNAVRLSIRRRMAMVFQKPAVLNTTVAGNVAFGLK
                     FRKVEKSEIYSRVHEALDIVGLLHLEDRKAVTLSGGEMQRVAIARALVTRPEVLLLDE
                     PTANLDPVNTDLIEKLILAIHKKFHTTIILSTHDMIQGQRLADRMGVIVNGRIVQVGS
                     IHDIFYRPQNKDIARFVGIDTILKGIVNSNEDGHAKISVGDAVFEVITPFRPGTQVAL
                     FIRPEEVTIALKDGDLPRTSVRNQLTGKITKMVPFGPFVRTTIDFGPHLTALVTRRSA
                     DELSLSVGMTVIAGIKATAIHVMPDSA"
     gene            3701..>4321
                     /locus_tag="VFC43_04765"
     CDS             3701..>4321
                     /locus_tag="VFC43_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZK30530.1"
                     /translation="MRCTILASGSKGNCIFLEGQSGALLLDAGLSTKEALLRMDTSSL
                     DAGLIQAVLVTHEHGDHVRGLDVLARRLNVPIHATAGTLHDFLHHRRTSDKHIDHHAC
                     RYHERFGVGDFLIEPFATSHDAAEPCGFIIRENGLKFGYCTDTGIMTAQMLDLLRQCD
                     AIVLESNHCPEMLQNGPYPEMLKRRIRSKRGHMSNAAAAKCLQAFGK"
BASE COUNT         1109 a         1139 c         1096 g          977 t
ORIGIN      
        1 ggctgaaata catcacgctc cctgcagaca tagacctctc cgacccaaag atggaggcgg
       61 catacaagac catgcaggta aaacggccct cgggaaatac aacggtaact gaagtcggta
      121 taccgatcgt atacgcagcc actgtcccgg cgagtgcaag aacaccggat tcgggatatg
      181 aattcattaa cctgcttctc agtagcgacg gccaggcagt tctgaatacc gacggtcaga
      241 ctccgatcgt accggcagaa gcatacggta ctgtgccagg tgcgattctg cagaatgtga
      301 aaaaagtcta atcttttttt ctttttgact cgtttaccct gacattttcc ggatcaaagc
      361 gatccgttcc ggggtattat ctgacgatta attctattcc tttgtagttt tttagccggg
      421 tttttctgga tatagcacat cgcaaatgaa acgtcaggga aataatagct gtgacattca
      481 aggtgagaac tcagcacgtt ttctgcacct ttctcctgcc gacccaccta aggagagacg
      541 caataaccat cccgagaata aatgcgatcg tcatcgagct cacgagtgca agtattccaa
      601 tgatacaggt gactgcaaga gaatccgtac ggaggctgtg ccgggccatc tcgatcccga
      661 caaataccag cagtgctccg aggactccga ccgctatgat agagagtatc tgcggggagg
      721 tgaagaagag ggcgaggata ataaagatca agccagcgta gacattcgcc ccaccggtcc
      781 tcgcaccata gcggtactgc ccggcaagcc cgcctgctcc gtggcacatc ggaaatcccc
      841 cgaagggaac cgaagttaaa ttcatcagcc cgatggttgt ggaaagcttc tttggcggca
      901 catctgccga gaagagatct ttcgtcaaca gcgacgtggc gagaattgcg tttgcgatgg
      961 tgagaacaac ctgaggaaga acgagcgttg aaaacgccga tgaaaaatct gcaggaaggg
     1021 gaattaccag ctgcggcgca gggataaggc tgaggggggg tataccgttt aggagcagcc
     1081 ccccgatcaa cccgacacct ataacacaca gtgacgaaag atcgggaaca cgacggtaac
     1141 ggaagagaag aagaaatgcg atgacaatcc cgataccgag gataaaaaag aacgggtctt
     1201 tgatcacaaa acctgctgat gctttaagca ggagcaatgc aaggcccagc tgaatccccc
     1261 gcacgacact ctgtggcacc cagcggtcga taacctcaaa gactcgacca tagccgagca
     1321 caagaaagat caccccgagg ataagtccgg atgctgctat ctcaccgcgg ctgatacctc
     1381 cggcgatgac aatcactgca accgctttca ttggttcaag agggataggc aaccggtagt
     1441 aaagcccggt aagaataaac cagatgccaa agaagaggag cacgtaccgg gtgttgacat
     1501 ccgaaaccag cgccacggca attataagtg gaataatcgt cccgaaatca cccaaagagc
     1561 ccgcgagctc cgacagggag aagttcatcg gaattgcggg ctttggggac acggtgtatg
     1621 gtgacaatga tcaggaattt tggctggtca atatcgtccc ttcttgttcc tggttcgctc
     1681 cggatcgtaa catatacaaa aatcgtttac caatcattaa aataaagtaa tgctgatgat
     1741 aagaattaac cttttcacca atatagggcc acatatcggt aaaacaggaa acgaaacata
     1801 ataattccgg gggtataagc catgagcgag attgtatcag gattttttca ggcgatcaat
     1861 ctgatcgtca ctctcaaccc cgaagtaatg cagatagccg cactctctct cttcatctcg
     1921 cttgctgcaa ccattttcgc agccgttatt tccataccac tgggaggatt aatctatttt
     1981 cacgaatttc gtggcaaacg ggtgctcatc gttctcatcc agaccctcta ctccgtcccg
     2041 accgtggttg tcggcctcat actctacctc ttcctctcca gggacggccc gctcgggatt
     2101 tttgggctcc ttttcacccc ggaaggtatg atcctgggcc agaccttgct gattattccc
     2161 atcatgaccg gcctggtcat cgctgccctc ggcggggtag accggaatat cagcgatacg
     2221 cttatctcgc tgggagctac aggattccag gccattatcc atattgtcag ggaagcgcgt
     2281 ctggcaatcc tcagcgcagt ggtccttggc tttggtcgtg cgatctccga agtcggtgtg
     2341 gcgatgatga tcggtggcaa tatcaggggt gcgacacgcg tactgactac tgctatcgcg
     2401 ttgcagaccg gtatcggtga tttcccgttc tcaatcgctc tcggtatgat cctgctcctg
     2461 atagctcttg tcgtcgttat catcctgaat ctcatcacat ccggcctgtc gtttgagtcc
     2521 caggtggcac ttggaggccc gcgtacgtga tccggatagc agatctctcg cgccgggctg
     2581 gcagcaaaac aatcctcgaa ccgatcaacc tcgagatcca tcgtggggag atcttcacca
     2641 tcatcggccc gagcggaagc ggcaagacga cgcttttacg tcttatcgat cttctcgata
     2701 tgccaactac tggcaccatt gtgtttgatg gaactgatac cgctgcatcg aatgcagtcc
     2761 ggctttcgat ccgcaggcgc atggcgatgg tcttccaaaa acccgcggtc ctgaacacga
     2821 cagtcgccgg caatgttgca ttcgggctga agttccggaa agtagaaaaa tcagagattt
     2881 actctcgtgt tcatgaggcg ctcgatatcg tcggactcct gcaccttgaa gaccggaaag
     2941 cggttacact ctccgggggt gagatgcaac gcgtggcaat cgcacgggcg ctggtcaccc
     3001 ggcctgaagt cctcctgctg gacgagccaa cggcaaatct tgatccagtc aacaccgatc
     3061 tgattgagaa actcatcctt gccattcaca agaaattcca tactacaatc atcctctcca
     3121 cccacgatat gatccagggc cagcgccttg cagacagaat gggagtgatt gttaatgggc
     3181 ggattgtcca ggtcggttca attcatgata ttttttaccg tcctcaaaac aaggatattg
     3241 cccggtttgt cggcattgac acgatattga aaggtattgt gaactcgaat gaagatgggc
     3301 atgcgaaaat ctctgtcggc gatgcagtct ttgaagtaat tacacccttc agacccggga
     3361 cacaggtagc cctgttcatc cgccccgaag aagttaccat tgccctgaaa gacggtgatt
     3421 tgccaagaac cagtgtacgg aaccaattga caggaaagat aactaagatg gtaccctttg
     3481 gaccctttgt ccgcacaact attgacttcg gtccacatct gacagcactg gtgacccgca
     3541 gatctgctga tgagctttcg ctttctgtcg gaatgacagt tatagcaggc atcaaagcca
     3601 cagctatcca tgtaatgccg gattctgcct gatccgttca ttgtggtaaa aactgtaaaa
     3661 agtcaaacac tctaatacaa gaacgcccaa taataggacg atgcgctgta caatactggc
     3721 cagcggaagc aaaggaaact gtatttttct ggagggacag agcggggcac ttctcctgga
     3781 tgccggcctg agcacaaaag aggcgctcct gcgaatggac acatcgtccc tcgatgcagg
     3841 tctgatccag gcggtgcttg tcacccatga acatggtgac catgtcaggg ggcttgatgt
     3901 actcgcccgg cgccttaatg tccccatcca cgcaacggca gggacgctcc atgattttct
     3961 ccaccaccgc cggacttccg ataaacatat tgaccatcat gcatgccggt accacgagcg
     4021 gtttggagtc ggggattttt tgattgagcc ctttgccacg tcccatgatg cagccgagcc
     4081 ctgcgggttc atcatcaggg agaatgggtt gaagtttggt tactgtaccg acaccggtat
     4141 catgactgcg caaatgctcg acctgctccg gcagtgtgat gcgatcgtgc tcgaaagcaa
     4201 ccactgcccg gaaatgctgc agaacgggcc atatcctgaa atgctcaaac ggaggatccg
     4261 ttcgaaacgc gggcacatgt ccaatgctgc cgctgcaaag tgcctgcagg cctttggcaa
     4321 a
//
LOCUS       DASRLV010000119         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1010759,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000119 DASRLV010000000
VERSION     DASRLV010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010759"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /locus_tag="VFC43_04770"
     CDS             complement(<1..1281)
                     /locus_tag="VFC43_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZK30531.1"
                     /translation="MESPDQLPKNYDFAEVEERWRGTWRDEDHYFDRDSKKPQFVIDT
                     PPPYPTGNFHIGNALNWCYIDFFARYKRMKGYNVMFPQGWDCHGLPTEVKVEEINHIT
                     KNDVSREEFRRMCHELTEKNISLMRLTLRKMAFSTDWSNEYVTMKPEYYGKTQLSFLR
                     MLKSGYIYQSEHPVNYCIRCETAIAFAEVAYDDRNTRLNYFDFDGVEIATTRPELLAA
                     CVAVAVHPTDERYHHLKGRTMKVPLFGHEVPVVLDEAVDPAFGSGAVMICTFGDKQDV
                     HWWKQHNLALRKAIDRQGKMTGIAGKYEGMNTTDCRAAILDDMKDKKILKRQEHLAQR
                     VGACWRCKTPIEILSERQWFVKIKPDEIQKAAHQITWYPEHMLLRMENWVEQMEWDWC
                     ISRQRIFATPIPVWFCKKCGDMVLPDEKDLPVDPT"
     gene            1421..>2481
                     /locus_tag="VFC43_04775"
     CDS             1421..>2481
                     /locus_tag="VFC43_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HZK30532.1"
                     /translation="MVKQRGLGFAAGFVSGAILIAPYVQPAWLMSLVVILFSLLLWRF
                     FNTKYLTYSFCVIAALYGVTLLPLFVFCCTLAMLVLGELVFEHGADDLNTYLYYIIST
                     SWAGVLVIAYLKQEYFLTVIFGIIAAVLLKTILLKFEDSLVIEAIGIAMTMWLIRELN
                     YQADLLLVVIAVFVGFTFGYFAFRAKTADLSGLFSAALVGTILLVFATPKGTQWFLIM
                     LSFFILGSAATKYKYQYKKRIGVEQGQSGARGYRNVFANGIIAAAAAVLFGVFQMPVF
                     IVLYVGCVATAAADTLASEIGVTGGIPYMITTFKKVPIGTNGGVTLTGELVALLGGFT
                     ISLVAFLLNVITPEMMVICT"
BASE COUNT          532 a          640 c          618 g          691 t
ORIGIN      
        1 ggtgggatcg accggcaggt ccttttcatc cggcagcacc atgtcgccgc attttttaca
       61 gaaccagacc gggatcgggg ttgcaaaaat cctctgccgc gagatgcacc agtcccattc
      121 catctgttcg acccagtttt ccatcctgag gagcatatgc tccgggtacc aggtgatctg
      181 gtgagctgct ttctggatct catccggttt tatttttaca aaccactggc gctcggaaag
      241 gatctcaatg ggggtcttgc agcgccagca ggctcctacc cgctgggcaa ggtgttcctg
      301 gcgcttcagg atttttttat ccttcatgtc gtcaaggatt gcagcacggc aatcggtcgt
      361 gttcatgccc tcatatttcc ccgcaatgcc ggtcatctta ccctgccggt cgatggcctt
      421 tctcagtgca aggttgtgct gcttccacca gtggacgtcc tgtttgtcac caaaagtaca
      481 gatcatcacg gccccggaac caaatgcggg atcaactgcc tcatccagca cgaccggaac
      541 ctcgtggcca aacaagggga ctttcatagt cctgcctttc aggtgatggt accgttcatc
      601 agtcgggtgt actgcgactg ctacacatgc ggcaaggagt tcaggacggg tagttgcgat
      661 ctcgacgccg tcaaagtcaa agtaattcag ccgggtattc cggtcatcgt acgcaacttc
      721 ggcaaatgca atcgccgtct cgcagcggat acagtaattg accgggtgtt cactctggta
      781 aatatacccg cttttgagca tgcgtaaaaa cgagagctgg gtcttgccgt agtattccgg
      841 cttcatcgtg acgtactcgt tgctccagtc tgttgagaac gccattttcc gtaacgtgag
      901 gcgcatcagc gatatgtttt tttccgtgag ttcatggcac atcctcctga actcctcgcg
      961 ggaaacatcg tttttggtaa tatggttaat ctcttctact ttcacttccg tagggagtcc
     1021 gtgacaatcc cacccctggg gaaacattac gttatatccc ttcatgcgtt tatagcgggc
     1081 gaaaaagtcg atatagcacc agttcaacgc attgccgata tggaagttac ccgtggggta
     1141 gggcggggga gtgtcgatca cgaactgtgg ttttttcgag tcccggtcga aatagtgatc
     1201 ttcgtcgcgc caggtgccgc gccagcgctc ctccacttca gcgaaatcgt aatttttcgg
     1261 cagttgatca ggagattcca ttcgccagaa ggttggatgt gaaataaaat atagtgatcg
     1321 tattcacgct tagagagaca tttcagagaa tggcaacggg tttttataaa aagccggcac
     1381 gataaaactc tttttgctca aagaaacaaa tgattgataa atggtcaaac aacgaggact
     1441 gggatttgct gcaggttttg tcagcggtgc catcctgatt gccccgtatg tccagcctgc
     1501 atggctgatg agtcttgttg tcattctctt ctccctcctc ctctggcgtt tttttaatac
     1561 caaatatctt acctattctt tttgtgtaat cgcagcattg tacggggtca ccctcctccc
     1621 gctgtttgta ttctgctgca cccttgccat gctcgtgctt ggagaactgg ttttcgagca
     1681 tggcgcagac gatctgaaca cctacctcta ttatatcatc tccacttcat gggccggtgt
     1741 cctcgtcatc gcgtatctca aacaggaata tttcttaacc gttatctttg gcatcattgc
     1801 agcagtcctc ttaaaaacaa tcctgctcaa attcgaagat tccctggtaa ttgaagcgat
     1861 cggtattgca atgacgatgt ggctgatcag ggagctgaat taccaggcgg acctcctgct
     1921 ggttgtcatc gctgtttttg tcggatttac cttcgggtat tttgctttcc gggcaaaaac
     1981 cgcagatctt tcaggtctgt tctctgctgc tcttgtcggg actatcctgc ttgtcttcgc
     2041 aacacccaag ggtacgcagt ggtttttgat catgctctct tttttcatcc tcggctctgc
     2101 tgcaaccaaa tataagtacc agtacaaaaa gaggatcggc gttgaacagg gacagagcgg
     2161 agcccgggga taccggaatg tttttgcgaa cggaattatt gcagccgctg cggcagttct
     2221 tttcggcgtc tttcagatgc cggtattcat cgtgttgtac gttggctgtg ttgcgaccgc
     2281 tgctgccgac accctggcaa gcgagattgg ggtaaccgga gggatcccct atatgattac
     2341 cacgttcaaa aaagtcccga tcgggacgaa cgggggtgtt accctgaccg gggaactggt
     2401 agccctgctg ggaggcttta ccatttccct ggtcgccttc ctactcaacg tgattacccc
     2461 ggaaatgatg gtcatctgca c
//
LOCUS       DASRLV010000120         4699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_929813,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000120 DASRLV010000000
VERSION     DASRLV010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929813"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1975
                     /locus_tag="VFC43_04780"
     CDS             <1..1975
                     /locus_tag="VFC43_04780"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HZK30533.1"
                     /translation="YRPGQRHMLEVVAQCAREGGITMIDAPTGSGKSSVVASLLAERK
                     GRKVVIAVRTVSQLTTFIRELELVKKKQPLLKTVYLVGKSSMCPLGGEGDIYRRCEGV
                     KAFSSSLMRERAEKGALIPAKDPFIIQQVRRMDRDHPLICPYFINSKMFVQADTVGVK
                     MVPSTALRTKADRVIAHMIVPRELGEFCGDLCPYELMMHAARNTDVVILNYHHLFDRD
                     IREQLYANLGVEPQDILLLIDEAHNCGDVITGIESVTLEERDLEQASRELSGMRKRHK
                     GADAVQHVLPRLTEFIRGLENSGEVEDWFDPLIFDRMIIRESLYKNMDEIVDDLIGLS
                     ENIREKNLKAGEYRETAIERLTEFLFRLSQSATDPAYLTVYRKDPEGITLEVRNIDPA
                     VPLQEICTAHACSILISGTLSPVDSFARLYFGNTPVKRLALPNAFPKENRLVCCAKDI
                     TTAFSMRQNKENNARIVEYIRAFCALKGNRAVYFPSYQILETYAGLTVPHIKNRKIFI
                     EPRDAADAGAALSTFLSLPAQGESGILLAVCGGKWSEGLDYRGEMLNGAMVIGLPLAP
                     FNRVRKMVIEYFRHKFGPEGEFLCYTLPAINRSLQALGRVLRTPEDRGVLVLGEKRFL
                     EMRVHGALPAWIKEEMIACDTARFREVISPWKS"
     gene            1963..2412
                     /locus_tag="VFC43_04785"
     CDS             1963..2412
                     /locus_tag="VFC43_04785"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZK30534.1"
                     /translation="MEIIGGSCRFGLWYVHIWWTRSTIHRIRFAITGIPGEVPDLVRQ
                     YCSGKAVSLLPLDSSVTHDDSVYGRIYREVRQVPYGSTSTYGEIAKKIGTSPRVVGQA
                     MARNPTPLVIPCHRITAAHGIGGFSPSIDIKEALLAMEKKNVAKAGK"
     gene            2504..3139
                     /locus_tag="VFC43_04790"
     CDS             2504..3139
                     /locus_tag="VFC43_04790"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HZK30535.1"
                     /translation="MRSAVVLVGGQARRANGQEKYFFTYQGRTFIERLVDSLKEVVDE
                     IILVARNPEQCRRFEGVEGVRCITDIRTGLGPIGGLHAGSVAAQGDLIFVSACDMPCI
                     NPAVISYLFERIDGYDAAIPVWNPDMLEPLHAVYRKTALVTYLESHDSLSLRAMIMNL
                     SARYIPVDDIKKIDPDLLTFTNINKLEDLEHMNRAGSKHQVFSPKNEKNYK"
     gene            3258..4109
                     /locus_tag="VFC43_04795"
     CDS             3258..4109
                     /locus_tag="VFC43_04795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZK30536.1"
                     /translation="MEHLSVENREVLRLFLVASACIGAFLTSIFSLMNGIFSVFPFHY
                     ILPIILVVYLYPERGVLFSLGLSLMYIGLIYLLGNSDPTQIAIATAWFAIFITIGVVA
                     SSYAIRQREERTRVRNILDHSQDGIFCFDLQNQKIREINPKCAQWLKYNTSELVGKDI
                     SAIWIDTEEQKQFIADAQQETRQTHTPREAWFRGHDGALYRFAISPILVTATHIMCSA
                     IDITRSKIIDEEIIKTLDDLEHQVKDRTADLARMNEQLRAEILECRRFESTVLSGHPL
                     QPKREDV"
     gene            4106..>4699
                     /locus_tag="VFC43_04800"
     CDS             4106..>4699
                     /locus_tag="VFC43_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZK30537.1"
                     /translation="MKGPLSSVKPRYFWITMIVMSVIIAIYGSVFSLSHGIYEVFPFL
                     YFLPIILFVNFYPNRGVIFSLSLSAVFLLLVYFFSNFDPALTAVSTAWFVIFVTIGFV
                     TSSFAEGSRAEERKYHEIFENSQAGIFTFALTTLRIQEMNGKCAHMLGYDRQDLIDKD
                     LSRILLDSAERDSFISQIQKNSGISDVELLLHTREGLV"
BASE COUNT         1160 a         1203 c         1202 g         1134 t
ORIGIN      
        1 atatcgcccc ggccagcgcc atatgctgga agttgtggca cagtgtgcac gggaaggcgg
       61 gatcacgatg atcgacgccc ccaccggcag cggaaagtca agtgtggtcg cttcccttct
      121 tgcagaacgg aaagggagaa aggtggtcat tgccgttcgc accgtcagcc agctcaccac
      181 attcatccgc gagcttgaac tggtgaagaa gaaacaacct cttttaaaaa cagtctatct
      241 tgtaggaaaa tcgagcatgt gcccgcttgg gggtgaaggg gatatctacc gcaggtgtga
      301 gggagtaaag gccttttcct cttcactcat gcgggaaagg gctgagaagg gtgccctgat
      361 accggctaaa gacccgttca tcatccagca ggtccgccgg atggaccggg accatcccct
      421 gatctgcccg tatttcatca acagcaaaat gtttgtgcag gctgacacgg tcggggtcaa
      481 gatggtcccc tcgacagcac tccgtaccaa ggcagaccgg gtcattgcac acatgatcgt
      541 cccgcgggaa ctgggagaat tctgcggcga tctctgtccc tacgagctca tgatgcatgc
      601 tgcccggaat accgatgtcg tcatattaaa ctaccatcac ctctttgacc gagatattcg
      661 ggaacagctc tatgcaaacc ttggtgttga gccccaggat atcttactcc tcattgatga
      721 ggctcacaac tgcggggatg tcatcactgg tattgagagc gtgacactcg aagaacgcga
      781 tctggagcag gcatcccggg aactctccgg catgcgtaaa cgccataaag gtgccgatgc
      841 ggtccagcac gtactcccaa gactcaccga gtttatccgc gggcttgaaa actccggtga
      901 ggttgaagac tggttcgacc cgttgatatt tgaccggatg atcatacgcg aatccctcta
      961 taaaaacatg gatgagatcg ttgacgacct gatcggactt tccgagaaca tacgggaaaa
     1021 aaatctaaaa gccggagaat atcgcgaaac agcgattgag cggctgacag aattcttatt
     1081 ccggctctcc cagtccgcaa cggaccctgc gtatcttacc gtgtaccgga aagatccgga
     1141 ggggatcacg ctggaagtgc gcaatatcga tcctgcggtc cctctccagg agatctgtac
     1201 ggcgcatgcc tgcagcatcc tcatctcggg caccctttcg ccagtcgaca gcttcgccag
     1261 gttatacttt ggcaataccc cggtgaagag acttgccctg cccaatgcat tccccaaaga
     1321 aaatcgtctt gtctgttgtg caaaggatat taccactgca ttctcgatgc ggcagaacaa
     1381 ggaaaataat gcacgaatcg ttgagtatat cagggcattc tgtgcactca aaggtaaccg
     1441 ggctgtctat tttcccagtt accagatcct tgaaacctat gcgggcctga ctgtcccgca
     1501 tattaaaaac aggaagatct ttatcgagcc gcgggatgct gcggatgccg gcgcggcact
     1561 ttccacattc ctgtcccttc ctgcacaggg ggagtcggga atcctgttgg cagtttgcgg
     1621 cggaaagtgg agcgaagggc ttgactaccg cggggagatg ctgaacgggg caatggtaat
     1681 cggactgccg cttgcaccgt tcaacagggt ccggaaaatg gtgattgaat acttccggca
     1741 caagtttggc cctgaaggtg agttcctctg ctacaccctg cccgccatca accgctccct
     1801 ccaggcattg ggaagagtgc tgcgtacacc cgaagaccgc ggtgtgcttg tgctggggga
     1861 gaagaggttt ctggaaatga gggtgcacgg cgcacttcct gcctggatca aagaagagat
     1921 gatcgcctgc gacacagccc ggttccgcga ggtgatttca ccatggaaat cataggaggt
     1981 tcctgccggt tcgggctctg gtatgtccac atctggtgga ccagatcgac gattcatcgt
     2041 atcaggtttg caataacggg tatcccggga gaagtgccgg acctggtaag gcagtactgt
     2101 tcaggaaagg cagtcagcct tttgcccctc gacagttcag tcacccacga cgattcggtt
     2161 tacggaagaa tttaccggga ggtccggcag gtaccgtatg gttccacctc aacatacggg
     2221 gaaattgcaa aaaagatcgg gacttccccc cgtgtagtcg ggcaggctat ggcacgcaac
     2281 cccacccccc ttgtcatacc ctgtcatcgt atcaccgcgg cgcatggtat cggcgggttc
     2341 tctccatcca ttgatatcaa ggaggcactg ctcgccatgg aaaaaaagaa tgtggcaaag
     2401 gcggggaaat gaccgttcac cgtgcctctt tgtgtacctc agcaaggtat tcctgccggc
     2461 aggataatac cagacctgat aagaaaataa acggagcacc ggagtgagat cagctgttgt
     2521 cctggtggga ggccaggccc gtcgtgcaaa cgggcaggag aaatattttt ttacctacca
     2581 gggcaggaca ttcattgaac gcctggtcga ttcgctcaaa gaggttgtcg acgagataat
     2641 ccttgttgcc aggaacccgg agcaatgcag gcgctttgag ggggttgagg gggtacggtg
     2701 catcactgac atccgtacag gtcttgggcc gattggaggc cttcatgccg gatccgttgc
     2761 tgcacaggga gatctgatct ttgtctccgc gtgcgatatg ccctgcatta atccggcggt
     2821 aatatcctac cttttcgagc gtatcgacgg ctatgatgct gcgatacctg tctggaaccc
     2881 cgatatgctc gaacctcttc atgcggtcta ccggaaaacc gcgctcgtca cctaccttga
     2941 aagtcacgat tcactgtcgc tccgggcaat gatcatgaac ctttctgccc gctatatccc
     3001 ggttgacgac ataaaaaaaa ttgatccgga tctcctgaca ttcaccaata tcaacaagct
     3061 tgaagatctt gagcatatga atagagcagg ttcgaaacac caggttttct cgccaaaaaa
     3121 cgaaaaaaat tataagtgat ccgtagattt gtggcaaatt accacagagt atataaatat
     3181 atgtaattac atcattaatt agtagtatat caggagccgg tcatcagact ggctgggaat
     3241 tcggcagggg acggccagtg gagcatttat ctgttgaaaa cagggaagta ttacgactgt
     3301 tcttggtcgc ttccgcatgc ataggcgcat tccttacgtc gatattttct ctcatgaacg
     3361 gtatcttcag tgtctttccc ttccactaca tcctgcccat tattctcgtt gtctacctgt
     3421 accctgaacg gggtgtactg ttttcgctgg gattaagcct gatgtacatc ggtcttattt
     3481 accttttagg gaattcagat cctacacaga tcgccattgc aacggcatgg tttgcgattt
     3541 ttatcacgat aggtgttgtc gcatcatcct atgcaatccg gcagcgggag gaacggaccc
     3601 gtgtcagaaa tatccttgac cattcccagg atggaatttt ctgtttcgat ctccagaacc
     3661 agaagatacg cgagatcaac cctaaatgcg ctcaatggct gaaatacaat acgtcagaac
     3721 ttgtcggaaa ggacatatca gctatctgga ttgacacaga agaacaaaaa cagttcatcg
     3781 ctgacgcaca acaggaaaca agacaaacgc ataccccgcg ggaagcctgg ttccgtggtc
     3841 atgatggcgc tttgtaccgc ttcgccattt ctcccatact ggtaaccgcg acccacataa
     3901 tgtgttctgc catcgatatc accaggagta aaatcatcga tgaagagatt ataaaaacgc
     3961 ttgatgatct tgaacaccag gtaaaagacc ggaccgcaga ccttgcacgg atgaacgagc
     4021 aactacgggc agaaatcctt gaatgccgga ggtttgaaag cacggttctt tcgggacatc
     4081 ctctccagcc aaaaagggag gatgtatgaa gggtcctctc agcagcgtca aaccccgcta
     4141 tttctggata acgatgattg tgatgtcagt catcattgca atctacggat ctgttttttc
     4201 cctgtctcac ggcatctatg aggtttttcc gtttctttat ttcctgccca tcatcctttt
     4261 tgtgaatttt tatccgaacc gtggggtgat cttctcttta tccctcagtg ccgtctttct
     4321 cctgctggtc tattttttca gtaatttcga ccccgccctt acggctgtat ccacggcatg
     4381 gtttgtaatt tttgtaacta tcgggtttgt gacgtcatct tttgcagaag ggtcaagggc
     4441 agaagagaga aaataccatg agatattcga aaactcccag gcgggaattt tcacctttgc
     4501 ccttactacg cttcgcattc aggaaatgaa cgggaaatgc gcacatatgc tgggatacga
     4561 ccggcaggac ctgatcgaca aagacttaag ccgcattctt ctggattctg ccgagaggga
     4621 ttcgttcatc agccagatcc agaagaattc cggtatcagc gatgttgaac ttctccttca
     4681 tacacgcgag ggtctcgta
//
LOCUS       DASRLV010000121         3011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_607215,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000121 DASRLV010000000
VERSION     DASRLV010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607215"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1771
                     /locus_tag="VFC43_04805"
     CDS             <1..1771
                     /locus_tag="VFC43_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HZK30538.1"
                     /translation="GLEILVGGRIDPTFGKVLTIGLGGTLVELIRDVSLRILPVSPED
                     IDAMIQELKGYPLIKGFRNEPPRDKNGLIALIDTVARFFMNSPNVIEFDLNPVFLYEK
                     GACVVDARIYVSDEKTEIRRSQKALLSPDIFKAKSIAVVGASPEPNKVGYAVLRNLLT
                     FPGKVYPVNVKHEKILGRDVWRSLAAIPDTVDIAVIVVPARYVPQVVKEAGEKGIPLV
                     IIISSGFRETGKAGSDLESQVIATAEQYNVRIMGPNCLGIMLPHQGINTTFDPVSPRP
                     GNIAFISQSGAIISTIVDWSLPEEIGFSAVISIGNQADLTFEDFLKYAMDDPYTKAVI
                     LYIEEIRDGKRFMEISRDLTLKKPVVVIKSGSSRIGQITAASHTGSLSGSYEVYMTAF
                     WESGIIPVRSMREAFQTAELLASEGYPKGIRALVISNAGGFAVLSSDYAERFGINLVD
                     FSPAIISELDAILPAEWNRHNPIDMVGDASADRFARTFDVMIKNQDAWDIAFVIAVPS
                     AISDPIRVANELVRFSKNTHKMIVGCMIGGDSMKTPLRILHDSGIPNFPDLEDAFRAV
                     GNICLHLCWEEYHGRNCDPKNRS"
     gene            complement(1779..>3011)
                     /locus_tag="VFC43_04810"
     CDS             complement(1779..>3011)
                     /locus_tag="VFC43_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZK30539.1"
                     /translation="ILSATPLGIFQVRNGLLVWTNPQFAEMLGQEESSLVGKEMVKIF
                     SNPEDYERVYRELQITRNPRGFCQAECKLAKKDSTSLACQLQVLTVDPRDFDKGGTFV
                     VTDISEKKKIADALKDSEAKYREVLQNTQSIIMRMDTLGNITFFNHFALSFFDFAADE
                     IMGKNVIGTIVPIKTRPGHDLSMMANDLGFNAEGYAVNISENVRRNGDRVWIAWINKA
                     IRDEKGHIIEILCIGNDITDRKRNGQVRISTDTWKDIVVAETDVKEEVFDAVFHICTE
                     ISIEGREGKSVGTTFLVGDADNVMAKSRQISINAFEGQKPESRIITNQDIKENIKEFA
                     QLDGAFVIDGDGYVRAQSRYITVDTSNVTLPKGMGTRHNSVAAMTQVTNTVGIVVSQS
                     GGGITIFKNGLILKKITL"
BASE COUNT          747 a          714 c          712 g          838 t
ORIGIN      
        1 agggcttgag atacttgttg gcggaagaat tgatccaaca tttggcaagg tcttaaccat
       61 tggcttgggg gggactcttg tcgaacttat ccgggatgtt tcattgagga tacttccggt
      121 cagtcctgaa gatattgatg cgatgatcca ggaactgaaa ggataccctc tgataaaggg
      181 tttccggaac gaacccccca gggataaaaa tgggctgatt gcccttattg acactgttgc
      241 ccggtttttt atgaacagtc ccaatgtcat tgaatttgat ctcaatccgg tatttcttta
      301 tgaaaaagga gcatgtgtcg ttgacgcccg gatctatgtg tctgacgaga aaactgaaat
      361 caggcgatca cagaaagcat tattatcacc ggatattttt aaagcgaaat cgattgccgt
      421 tgtcggggca tcaccggaac cgaataaggt cggatacgcg gtcctgcgaa acctgctcac
      481 ctttccggga aaagtatacc cggtaaacgt aaaacatgaa aaaattcttg gacgggacgt
      541 ctggcgctcg cttgcagcga ttcccgatac ggtggatatt gcagttattg ttgttccggc
      601 ccggtatgtg ccccaggtcg taaaggaagc gggagaaaaa ggtattcctc tggttatcat
      661 catttcttcc ggctttcgcg agaccggaaa agccgggtca gatcttgaat cgcaggtaat
      721 tgcgaccgca gagcagtaca atgtgcgaat catgggcccc aattgcctgg gcatcatgct
      781 tccccatcag ggtattaata ccacatttga ccctgtatcc ccccggcctg gaaatattgc
      841 ttttatatcg cagagcgggg cgattatctc gaccatcgta gactggagcc tgcccgaaga
      901 gattggtttt tctgcagtca tcagtatcgg caaccaggct gacctgacat ttgaagattt
      961 cttaaaatat gccatggatg atccgtacac aaaggcagtt atcctgtata ttgaggaaat
     1021 ccgcgatggc aaacggttta tggagatctc ccgtgacctt accctgaaaa aacctgtggt
     1081 ggtgataaag tcaggatcgt cccggatagg tcagatcacc gcagcatcac atacgggatc
     1141 gctctctggc agttatgagg tctatatgac agcgttctgg gagtccggaa tcattcctgt
     1201 ccgatccatg agggaggcgt tccagactgc agaactcctg gcatcggaag gatatccaaa
     1261 gggtattcgt gcactcgtaa tcagtaatgc cgggggattt gccgtcctgt catctgacta
     1321 tgctgagcgg tttggtatta accttgtaga tttctctcct gcgataattt cagaacttga
     1381 tgcaatcctg ccggcagagt ggaacagaca taatccaatc gatatggtag gggatgcttc
     1441 agcggacaga tttgcccgta cgtttgatgt tatgataaaa aaccaggatg cctgggacat
     1501 tgcttttgtg atcgccgttc cttcggctat atctgatcct attcgtgtgg caaatgagct
     1561 tgtccggttc tcaaaaaata cccacaagat gattgtaggt tgtatgatcg ggggagattc
     1621 gatgaagaca ccgcttcgca ttctgcatga ttccggcatt cccaatttcc ctgaccttga
     1681 ggacgctttc agagcggtgg gaaatatctg cctccatcta tgctgggaag agtatcacgg
     1741 gagaaactgt gacccgaaaa atcgttcctg aaccccggtt acagcgtaat ttttttaagg
     1801 attaacccgt ttttaaatat cgtgatcccc ccgccgctct gggacaccac gatgcctacg
     1861 gtgtttgtga cctgtgtcat tgcagcaact gagttgtgac gggtacccat ccctttggga
     1921 agcgtgacat tactggtatc gactgtgata tagcgactct gggcccggac atacccatcg
     1981 ccatcaatca caaaagcccc gtcaagctgc gcaaattcct tgatattttc cttgatatcc
     2041 tggtttgtga tgatccggga ctcaggtttc tgcccctcaa acgcattgat cgatatctgg
     2101 cgggatttcg ccatgacatt atcagcatcg ccaacgagga acgttgttcc aacagatttg
     2161 ccttcccttc cttcaatgga tatttcggtg cagatatgaa agactgcatc aaagacctcc
     2221 tcttttacat ctgtttcggc cacaacgata tctttccagg tatcggtact gatgcggacc
     2281 tgcccgtttc gtttccggtc ggtgatatcg ttcccgatac agagaatttc aataatatgc
     2341 cctttttcat cgcggatcgc tttatttatc catgcgatcc acacgcggtc tccattccgc
     2401 cggacattct cgctgatatt gactgcatag ccctcagcat taaacccgag atcatttgcc
     2461 atcatggaga ggtcgtgacc aggacgggtc tttatgggaa caatggtgcc tattacattc
     2521 tttcccataa tttcatcggc agcaaaatca aaaaaagaga gagcaaagtg attgaaaaat
     2581 gtgatatttc ctaaagtgtc catcctcatg ataatgctct gggtgttctg gagcacttca
     2641 cgatatttcg cctcactatc tttgagtgca tcggctattt ttttcttttc cgatatatcc
     2701 gtaacaacaa atgtcccgcc cttatcaaaa tcacggggat caaccgtgag cacctgcaac
     2761 tggcatgcga gacttgttga atcttttttt gccagcttac attccgcctg gcaaaatcct
     2821 cgggggttcc gggtaatctg aagttcacga tatacccttt cgtaatcctc aggatttgaa
     2881 aaaattttta ccatctcttt tccgacaagc gacgattctt cttgtcccag catttcggca
     2941 aattgtgggt ttgtccatac taagagacca ttccgtacct ggaatattcc aagcggagtt
     3001 gcggaaagga t
//
LOCUS       DASRLV010000122         9037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_40479,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000122 DASRLV010000000
VERSION     DASRLV010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 9037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9037
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40479"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VFC43_04815"
     CDS             complement(<1..261)
                     /locus_tag="VFC43_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286785.1"
                     /note="Directs the termination of nascent peptide
                     synthesis in response to the termination codons UAA, UAG
                     and UGA; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HZK30540.1"
                     /translation="MAEEREMDDAQKRYEFKKALEKLELQQGDGTELITIYIPPDKQI
                     FDVTNQLKDEYGQCANIKSKQTRTNVQSAISSILSRLKYYKRP"
     gene            complement(297..1190)
                     /locus_tag="VFC43_04820"
     CDS             complement(297..1190)
                     /locus_tag="VFC43_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZK30541.1"
                     /translation="MRTGIIIAAGILLVVFIASTGCMANKVSPSYSVDSTGILSVSCA
                     PVTYSEETLSRNETYTKTKIVLHTQDANVVTYLEAPQHPKAAVVYVPGAGEKPAGHEE
                     RMVRYSAEGFAFLFVDIRGNGGDTPGYALNPQRDFSLFEKGYWPQYYETICDLVTSQQ
                     MLSGRFGVPVYAMGSSNGGRYAAVATGVDPDFAGYVGISTSDWGILDSAIQAGETGDV
                     IRFASSLEPSTYFKKISPRPVWIFHSVNDPVIPFDSGKKFFLDAKEPKTFIEFFGEHG
                     INSDVDDRILMQWAQIYATRG"
     gene            complement(1194..1691)
                     /locus_tag="VFC43_04825"
     CDS             complement(1194..1691)
                     /locus_tag="VFC43_04825"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZK30542.1"
                     /translation="MEIFRGRKLWIEKRTIRLPNGIDREKIIVHPRNAVAILPVQGDR
                     CKLLKQYRYAVDQYIFEAPAGTMEEDEDPLATAHRELIEETGFVAKTIVPKGFIYTTP
                     GYTDEKIFLFEARDLSPSEEFGKDDDEIIEVMDVATSDLNDMIIKGTIVDAKTICLIH
                     RCLGC"
     gene            complement(1692..2357)
                     /gene="queC"
                     /locus_tag="VFC43_04830"
     CDS             complement(1692..2357)
                     /gene="queC"
                     /locus_tag="VFC43_04830"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HZK30543.1"
                     /translation="MKAVCLLSGGMDSSTLAFVAKSRGYDILGLHLNYGQRTERKELV
                     CAKKIASLLNAGTFIEISLDYFTKFGASSLTDEKIAVEDFDSTRAHMPNTYVPFRNAN
                     LLSIATSFAEAREADAIFIGVQSLDYSGYPDCRPQFIEAFQRVIDLGTKDTTKITLFT
                     PFINMTKTDILLEGIRLAVPYEYTWSCYRNEEKACGTCGSCHFRKEAFASVGKRDPIE
                     YEE"
     gene            complement(2357..2965)
                     /locus_tag="VFC43_04835"
     CDS             complement(2357..2965)
                     /locus_tag="VFC43_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZK30544.1"
                     /translation="MKVAEIFRSLQGEGIHQGKPCLFIRLAGCNLRCRWCDTSYAQNG
                     GIEMSVDSILEHARSLNSSYVCITGGEPLLQADELEQLLVPLSLKGAAIDIETNGTID
                     FTRMQPYASVCMDIKCPSSGERSDMGLLDALRPQDSVKFVVQDETDCLYARDIITSHN
                     IPAEIFFSPVSGSDYQPIVRFILVNDLPVRFQVQLHKLIGVK"
     gene            complement(2962..3366)
                     /locus_tag="VFC43_04840"
     CDS             complement(2962..3366)
                     /locus_tag="VFC43_04840"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HZK30545.1"
                     /translation="MKIGIYKEVHIDTSHRLLHYTGKCANLHGHRWRVEVWMEGEPDP
                     ATQILIDYSLIKQVIDKYDHQIILNRDDPMVPRIQEFHPVITTPGDPTSELMAVIIRD
                     DLYSVCRKQGIKATIPKIRVWESPTACAELTE"
     gene            3532..3960
                     /locus_tag="VFC43_04845"
     CDS             3532..3960
                     /locus_tag="VFC43_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HZK30546.1"
                     /translation="MGQGKFAARKLVRDSNKFRWADKNYARRELNLDVKSDPLEGAPQ
                     ARGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGRQVTAFAVGDGAINFIDEHDEVEIEG
                     IGGRLGRSMGDIPGVRYVVTKVNNVCLHEMVIGRKEKPRR"
     gene            3966..4544
                     /locus_tag="VFC43_04850"
     CDS             3966..4544
                     /locus_tag="VFC43_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HZK30547.1"
                     /translation="MKESEGKKLLFNKWDASEVTVKDPGLVRYVNLTALIIPHSCGKF
                     SKQEFNKANMPIVERLINRLMQTENNTGKKQMTIRIVREAFEIINKKTKRNPIEVLVE
                     AISNSGPREETVRLKYGGINVPKSVDTAPLRRVDSAIGFIAEATLKGSRSSKKKASAI
                     LADELIAASKGDMKCYSVGKKEERERIAKSAR"
     gene            4568..6763
                     /locus_tag="VFC43_04855"
     CDS             4568..6763
                     /locus_tag="VFC43_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor EF-2"
                     /protein_id="HZK30548.1"
                     /translation="MVRGKKSVERATELMKDPKHIRNIGIVAHIDHGKTTLSDNLLAG
                     AGIISEELAGKQLFMDSDAEEQARGITIDASNVSMVHEYEGQDYLINMIDTPGHVDFG
                     GDVTRAMRAVDGAVVLVDAVEGTMPQTETVLRQALKEQVRPVLFINKVDRLINELKVD
                     EIEMQIRLGKVIDKVNKLIKGMNEEAYNNGWKLDAAKGTVAFGSALYNWAVSAPFMKK
                     SGVSFKDVYEKCREGDMKYLAKNSPLSEVLLDMVVRTLPNPLEAQPRRVNVIWHGDKT
                     SPEGKAMLTCDPKGPVAMMVTDISFDPHAGEVATGRLFSGSLKRGDGLYVMGSAKKEN
                     RLQQVGIFMGPKRVEVEEIVAGNIAAVTGLRDAMVGSTVTSLLEMTPFESLKHYSEPV
                     MTVAVEAKNMKDLPKLVEVLRQVAKEDPTLVITINEETGEHLISGMGELHLEIITGRI
                     KRDKGVEIVTSEPIVVYRETVVQKVESVEGKSPNRHNRFYFDLQPLPDEIVDLIKKGE
                     VSMNQQMLERRDILMKAGMDKDEAKNIKDIKGTNMLIDKTKGVQYLNETMELIIEGIH
                     EALAGGPLADEPVQNLKMTLVDVKLHEDAIHRGPAQVIPAVRGAIKGGMLVAGDSLLE
                     PVQKIQITVPMDQMGAATSQIQGRRGQVFDMTSEGDTITVVGKAPVAELFGFSGDIRS
                     ATEGRAMWNTEFSGFELVPNSMLKEVVVAIRKRKGLKEVLPTPADYLAG"
     gene            6854..7363
                     /locus_tag="VFC43_04860"
     CDS             6854..7363
                     /locus_tag="VFC43_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZK30549.1"
                     /translation="MDQDKKYKIVVFGAFGAGKTTLIKTLDPQSSHVEAGCSGGTTTV
                     ALDYGRVQARGIHIHIFGTPGQERFEFAREILGRGMDGAILLVDATSPVDDFIHHLYD
                     SLAAAGIPLVIFLNKYDSIGARPELIHKEFSASVITKVSALDRQQSLEALCTFVQTLP
                     PHSGGHMHS"
     gene            complement(7506..7853)
                     /locus_tag="VFC43_04865"
     CDS             complement(7506..7853)
                     /locus_tag="VFC43_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30550.1"
                     /translation="MKSEFGWIEIAKTRYDHDVIIYTDGSVTRRLKKKSKDLKITYGH
                     TPLSENELEFLKSEQPEKVYIGTGQYGDLPLTPGAERVLNRFETIASPTPEILVMLEK
                     EQRPYSAIIHVTC"
     gene            complement(7922..8518)
                     /locus_tag="VFC43_04870"
     CDS             complement(7922..8518)
                     /locus_tag="VFC43_04870"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZK30551.1"
                     /translation="MSPLRTYILLSLLIAGFLICAGCTQSAPETTQATPSAGTATMTP
                     STEAQKLVKLETNIGTITIALAPDMPVTAGNFESLVKKGYYDGVIFHRVINGFMIQGG
                     DPTGTGMGGPGYVIKDEFTNNNRNDRGTISMANAGPNTGGSQFFINLVNNNFLDGKHP
                     VFGKVVEGMDVIDKIAKVKTDSSDRPAQNVTIIRAVMV"
     gene            complement(8712..>9037)
                     /locus_tag="VFC43_04875"
     CDS             complement(8712..>9037)
                     /locus_tag="VFC43_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZK30552.1"
                     /translation="VRAYSMKCQLMCDLLDDLLPQLSHTTPEGGMFLMATLPYGLSSM
                     TVFEEGIKQGVAVLPGLPFYVDGGGSDTIRMNFSTALEEQITEGMVRLARVITGLQKT
                     LARPE"
BASE COUNT         2320 a         2203 c         2263 g         2251 t
ORIGIN      
        1 agggcgcttg tagtatttga gccgggaaag gattgaggag atggcgctct ggacattcgt
       61 acgggtctgt ttgctcttga tattcgcaca ctggccgtac tcgtccttga gctgattggt
      121 aacatcgaag atctgcttgt ccgggggaat atagatggta attagttctg tcccgtcgcc
      181 ctgctgcaac tcgagcttct cgagcgcctt tttaaactca tagcgctttt gcgcatcgtc
      241 catctcccgt tcttctgcca ttgccggtct caaacctctc tagagctatt acatattcac
      301 ccacgtgtgg cataaatttg cgcccattgc ataaggatac ggtcatcaac atcggaattg
      361 atgccgtgtt ctccaaaaaa ttcaatgaac gtctttggct cttttgcatc caggaagaac
      421 ttcttcccgc tatcgaacgg gataaccggg tcattgactg aatggaagat ccagaccggg
      481 cgcggggata tttttttgaa gtacgtgctt ggttccaggg aagacgcaaa ccgtatcacg
      541 tctcccgttt ctccagcttg gattgctgaa tccagtatac cccagtcaga cgttgagatc
      601 cctacatacc cggcaaaatc cggatcaaca ccggtagcga ctgcggcata acggcctcca
      661 ttgctggatc ccatcgcata gaccggaaca ccaaaccggc ccgaaagcat ctgctgcgat
      721 gtaacaagat cacagatagt ctcgtagtac tgcggccagt aacctttttc gaacagggaa
      781 aagtcccttt ggggatttaa cgcgtagccg ggggtatccc cgccattgcc acggatatcg
      841 acaaacagaa atgcgaaacc ctctgcagaa tagcgcacca tacgctcttc atgtcctgca
      901 ggtttttcgc ctgctcccgg gacatataca accgctgctt tagggtgttg cggtgcttca
      961 aggtacgtaa caacatttgc atcctgcgtg tgcaaaacaa tctttgtttt tgtgtacgtc
     1021 tcgttcctgg acaatgtctc ttcactgtat gttacgggag cgcatgaaac tgaaaggata
     1081 ccggtactgt cgacagaata cgacggagaa accttatttg ccatgcagcc agtagatgca
     1141 atgaacacta caagaagaat tcccgctgct attattatac cagtacgcac agctcagcac
     1201 cccagacagc gatggataag acagatcgtc tttgcatcaa cgatagtacc cttgattatc
     1261 atgtcgttga gatcagatgt cgctacgtcc atgacctcga ttatctcatc atcatctttc
     1321 ccgaactctt cggaaggcga caagtcccgg gcttcgaata aaaagatctt ttcatccgta
     1381 tacccgggtg tcgtatagat gaaacctttt ggaacgattg tttttgctac aaagccggtc
     1441 tcttcgatga gttccctgtg tgctgtcgct aatggatctt catcctcttc catcgtacct
     1501 gccggggcct caaagatgta ttgatcgaca gcgtaccggt actgcttcag caatttgcat
     1561 cggtcacctt gaaccggaag tatggctacg gcattacgag ggtgaacgat tatcttctca
     1621 cggtcaattc cattgggaag ccggatcgtg cgtttctcga tccataactt cctgccacgg
     1681 aagatctcca tttattcctc gtactcgata ggatcccgct ttccaactga agcgaatgct
     1741 tctttgcgga aatggcacga cccgcaggta ccgcaggctt tttcctcatt gcggtaacag
     1801 gaccaggtat attcataggg gacagcgagc ctgatgcctt caagcaggat gtcagtcttt
     1861 gtcatattga tgaagggagt aaaaagggta atttttgtcg tgtcctttgt tcccaaatcg
     1921 atcacgcgct ggaatgcttc aatgaattgt gggcgacagt caggataccc gctataatcg
     1981 agtgactgga ccccgataaa aattgcatct gcttcccgtg cttcagcaaa actcgttgct
     2041 atcgagagca ggtttgcatt ccggaacggg acgtaggtgt taggcatatg ggcccgcgtg
     2101 gaatcaaaat cctctaccgc aattttttca tcagtaaggc tgcttgcccc aaactttgta
     2161 aaatagtcca ggctgatttc gatgaacgtg cctgcattca ggaggctggc aatttttttt
     2221 gcacaaacca gctcttttcg ctcagtccgc tgcccgtagt tcaggtggag cccaaggatg
     2281 tcataccccc tgctctttgc gacaaatgca agagttgaag agtccatacc tccggagagt
     2341 aaacaaacag ctttcatcat ttcaccccga tgagtttatg caactggacc tggaatctta
     2401 ccgggagatc attgaccagt atgaaccgta ctattggttg gtaatcgcta ccagatacag
     2461 gagagaaaaa gatttcagcg ggtatattgt ggctcgtgat aatatcccgg gcatacaggc
     2521 agtcagtttc gtcctgtacg acaaacttaa ccgagtcctg cgggcgtaaa gcatcaagaa
     2581 gccccatatc gcttcgctct cctgatgacg gacatttgat atccatacat actgatgcat
     2641 acggttgcat ccgggtaaaa tcgattgtgc cattcgtctc aatatcgatc gcggctccct
     2701 tgagggaaag cgggacaagc agctgttcga gttcatcagc ctgcaggagc ggttctcccc
     2761 cggtaatgca gacataggaa gaattcaggc tccgggcatg ttcaagaatg gaatcgacac
     2821 tcatctcaat tccaccattt tgcgcgtacg atgtatcgca ccagcggcag cgaagattgc
     2881 agccggcgag gcggatgaac agacagggtt ttccctgatg tatcccttca ccctgcaggc
     2941 tcctgaaaat ttcagcgact ttcattcggt aagctcggca caagctgtcg gggattccca
     3001 gacgcggatt ttcggtattg ttgctttaat cccctgtttc cgacagacag aataaagatc
     3061 atcgcgaata ataactgcca tcagttcact tgttggatct cccggtgtag tgataaccgg
     3121 gtggaactcc tgtatgcggg gcaccatggg gtcatcgcga ttgaggataa tctgatgatc
     3181 atatttatcg atgacctgtt ttatcagact ataatcgata aggatttgag ttgccggatc
     3241 tggttcccct tccatccaga cctcaaccct ccatcggtgc ccatgaaggt tggcgcattt
     3301 gccggtataa tggagaagac ggtggctggt atcaatatgc acctctttgt agatccctat
     3361 tttcattgac taatataggc tatagaagga tataagatta ttatgagtaa gggataatag
     3421 tataagacag gcaggtgggg tgccacaatt tataaattgg ttacccgcga ttgtatatat
     3481 ctcagtgcgg gttatcgata accacaaaaa caacaaaaat gaggctattt aatgggacag
     3541 ggtaaatttg cagccagaaa attggttcgg gactcaaata agttccggtg ggcggataaa
     3601 aactatgccc gccgtgaact caatctcgat gtgaagtcag accctcttga gggtgctcca
     3661 caagcgagag gtatcgtgct ggaaaaggtc ggtgttgagg caaaacagcc caactctgcg
     3721 atccgcaagt gcgtgcgtgt acagctgatc aagaacgggc gtcaggttac tgcatttgcg
     3781 gtgggcgacg gtgcgatcaa ctttatcgat gagcacgatg aagtcgagat tgagggtatt
     3841 ggcggccgac tcgggcgttc catgggagat attcccgggg tccgttatgt cgttaccaaa
     3901 gtgaacaatg tctgcctcca tgaaatggta attggcagga aggagaaacc gcgcaggtga
     3961 tcaccatgaa agaatcagaa ggaaaaaaac tactgtttaa caagtgggac gcgagcgaag
     4021 ttactgttaa ggacccgggt cttgtgcgct atgtgaacct gaccgccctg atcatccccc
     4081 actcctgcgg caaattctcc aagcaggagt tcaacaaggc aaacatgcct atcgtcgagc
     4141 gcctgataaa ccgccttatg cagaccgaga ataataccgg gaagaagcag atgaccatcc
     4201 ggatcgtgcg ggaagcattt gagattatca acaagaagac caagcgcaac cctattgaag
     4261 tcctcgttga agcaatttca aattccggcc ccagggaaga gacggtccgt cttaaatatg
     4321 gtggcatcaa tgtcccgaaa tctgtcgaca ctgcacctct gcgcagggtc gattccgcaa
     4381 tcggttttat tgctgaagca acgctgaagg gatcgcgttc aagcaaaaag aaagcgagtg
     4441 caatccttgc cgatgaactg attgccgcct caaaaggcga catgaaatgt tattcggtag
     4501 ggaaaaagga agaacgcgag cgcattgcaa aatccgcccg ttaatatctt ttttttgagg
     4561 ttatttcatg gtccgcggca aaaaatctgt tgagcgcgca actgagctca tgaaggatcc
     4621 gaagcatatt cggaatattg gaattgtagc acatatcgat cacggtaaga cgactctctc
     4681 cgacaacctg cttgcaggtg ccggtattat ttcagaagaa cttgccggca agcaactctt
     4741 catggattca gatgcagagg agcaggctcg tggtattaca atcgacgcct cgaacgtctc
     4801 gatggtgcac gaatatgaag gtcaggatta cctcatcaac atgattgata ccccagggca
     4861 tgtcgatttt ggtggagatg tgacccgcgc aatgcgtgct gtcgatgggg cagtcgtcct
     4921 ggtcgatgct gttgaaggca ccatgcccca gacagagacc gtgctgcgcc aggcattaaa
     4981 ggagcaggtg cgccctgtcc tcttcatcaa caaggtcgac cgactcatca atgagctgaa
     5041 agttgacgag attgagatgc agatccggct tggcaaggtt atcgataagg tcaacaagct
     5101 gatcaagggg atgaacgagg aagcatataa caacggctgg aagctggacg ctgcaaaagg
     5161 aaccgttgcg ttcggctctg ctctttataa ctgggcagta tctgcacctt tcatgaagaa
     5221 gagcggtgtc tcattcaagg acgtttatga aaagtgccgt gaaggggata tgaaatatct
     5281 ggcaaagaac agccccctct ctgaagtcct tctggacatg gtggtacgca cactccctaa
     5341 ccccttagaa gcccagcccc gccgtgtcaa cgttatctgg cacggtgaca agacatctcc
     5401 tgaaggcaag gctatgctta cctgcgatcc aaagggcccc gtcgcaatga tggtcactga
     5461 catctccttt gatcctcacg caggtgaagt tgccacggga cgtctcttct ccggtagcct
     5521 caagcgtgga gacggacttt acgttatggg ctcggcaaag aaggagaacc ggctccagca
     5581 ggtcggtatc tttatgggtc ccaagcgtgt ggaagtcgaa gagatcgttg cggggaacat
     5641 cgccgcagtt accggtctta gggacgcgat ggtcggctca acggttacct cccttttgga
     5701 aatgacaccg tttgagtccc tcaagcacta ttctgaaccg gtcatgacag ttgccgtaga
     5761 ggcaaagaac atgaaggatc tgcccaagct cgttgaagtg ctccggcagg ttgcaaagga
     5821 agacccgaca ctggtcatca ccatcaatga agagaccggt gaacacctga tttcgggtat
     5881 gggcgaactg caccttgaga tcatcaccgg ccgtatcaag cgcgacaagg gtgtggagat
     5941 cgtcacttca gaaccaatcg ttgtctaccg tgaaactgta gtccagaagg ttgaatccgt
     6001 ggaaggcaag tccccgaacc gccataaccg gttctacttc gatctccagc cactgcccga
     6061 tgagattgtc gacctgatca agaaaggcga agtctccatg aaccagcaga tgctcgagcg
     6121 ccgtgatatt ctcatgaagg cgggtatgga caaggacgag gcgaaaaata tcaaggacat
     6181 caagggcacc aatatgctca tcgataagac caaaggtgtc cagtacctca acgagaccat
     6241 ggaactgatc attgaaggta tccacgaggc gctcgccggg ggaccactcg cagatgagcc
     6301 ggtccagaat cttaagatga cactcgtcga tgtcaagctt cacgaggatg cgattcaccg
     6361 gggtcctgca caggttatcc ctgctgtccg tggtgcaatc aagggtggca tgctggttgc
     6421 cggcgattcc ctattagagc cggtccagaa gattcagatc acggtcccga tggaccagat
     6481 gggcgcggcg acttcccaga tacagggacg gcgtggccag gtctttgaca tgacgagcga
     6541 aggcgacacg attaccgtcg ttggcaaggc accggttgcc gagctcttcg ggttttcagg
     6601 tgatatccgc tctgcgaccg aaggccgtgc catgtggaac acggagtttt caggatttga
     6661 actcgtgccg aacagcatgc tcaaggaagt tgtagttgcg atccgcaagc gcaaagggct
     6721 caaagaagtc ctaccgaccc cggccgatta tcttgctgga taaaacccat tccccgaccc
     6781 tcttttaatt tattacctgc atcgggtatc tgtcaccgaa atggtaaaat cgcactccgc
     6841 cacatgtttt ctgatggatc aggacaaaaa atataagatt gtggttttcg gtgcctttgg
     6901 tgccggaaaa accacgttaa taaagactct tgacccgcaa tccagccatg tcgaagcagg
     6961 ctgttccggg gggacaacta ccgtggcact tgattacggt cgtgtacagg cacgtggaat
     7021 tcatatccat atttttggaa cgcccggaca ggaacggttt gaatttgccc gtgaaatcct
     7081 cgggcgagga atggatggtg ctatcctgct tgtcgatgca accagtcctg ttgacgattt
     7141 catccaccac ctgtatgatt cgcttgctgc ggcaggaatt cccttagtaa tttttttaaa
     7201 caaatacgat agcatcggtg cacgaccgga attaatccac aaagagttca gtgcatctgt
     7261 cattacgaaa gtttcagccc ttgacaggca acaatccctc gaggcattat gtacctttgt
     7321 acaaaccctg cctcctcatt ctggcgggca tatgcattcc tgattattac tgttgtcaca
     7381 cctgacagca attatggaaa agaaaagcat tacctgattt tgtcagaaat atcagcagat
     7441 acaccccggt gagtaaggat gggacaaaat gaacccataa aattttagcc tggataaccg
     7501 ggttttcagc atgtcacatg gatgatagca ctatacggtc gctgctcttt ttccagcatg
     7561 acaagaattt ccggagtcgg gctcgcaatg gtctcaaacc ggttaagcac tctttctgca
     7621 cccggcgtga ggggaagatc gccatattgt cctgtcccga tatagacttt ttccggttgt
     7681 tcggacttca aaaattccag ttcattttca gagagcgggg tatgtccgta tgtaatttta
     7741 agatcctttg attttttctt taaacgtctg gtaaccgaac cgtcggtgta aatgatcaca
     7801 tcatggtcat acctggtctt agcgatctca atccatccga attctgattt catatatgca
     7861 tggtcatatg caagacccca ataaaaaggg atcgggaaaa tgacaaaaat taaaaaaagt
     7921 ttcagaccat cacggcacgg atgatcgtga cgttctgggc cgggcgatca cttgaatcgg
     7981 tttttacctt ggcaattttg tcaataacgt ccatgccttc gacaaccttg ccaaagaccg
     8041 ggtgtttgcc gtcgaggaag ttattgttga caagattgat gaagaactgg cttcccccgg
     8101 tattcgggcc agcgtttgcc atagagattg taccccggtc attccggtta ttattcgtaa
     8161 actcgtcctt gatgacataa cctggtccac ccatgccggt gccggtcgga tcaccaccct
     8221 ggatcataaa tccattgatc acccggtgga atattacacc gtcataatag ccttttttaa
     8281 cgagcgattc aaagttgccc gcggtgacgg gcatgtcagg agcaagggca attgttatcg
     8341 tgcctatatt cgtctcaagc tttaccagtt tttgtgcttc tgttgatggg gtcatagtgg
     8401 ctgtcccggc cgatggtgtg gcctgagttg tctccggggc ggactgtgtg caccctgcac
     8461 agatgagaaa gcctgcgata aggagactca acaggatgta agtacgcaat ggtgacatca
     8521 gtatacagtt tggtggggat agaacataaa agatttcaga aagggtggga ttaaccgttt
     8581 aaaaaagggt aagttactct tggatgaatc cgcattttta gctacgtgca tgatggttat
     8641 gaccatcaga aaacaaatgc tgtcagttaa ttcaaacaca tcatatctga tggtcgaatt
     8701 cagacggttt actactccgg ccgggctaag gttttttgaa gaccggtaat caccctggcc
     8761 agtctcacca taccctccgt aatttgttct tccagagcag tcgaaaagtt catgcgtata
     8821 gtatcagaac cccccccgtc cacatagaaa ggcagacccg gtaacacggc gaccccttgt
     8881 ttaatcccct cttcgaaaac tgtcatcgat gacagcccgt acggcagtgt ggccatcagg
     8941 aacatgcctc cttctggtgt tgtatgtgac aactgtggca acaggtcgtc aaggaggtcg
     9001 cacatcagct ggcacttcat gctgtaagcc ctgacaa
//
LOCUS       DASRLV010000123         6191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_608741,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000123 DASRLV010000000
VERSION     DASRLV010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608741"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VFC43_04880"
     CDS             <1..309
                     /locus_tag="VFC43_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZK30553.1"
                     /translation="AIVLESNHCPEMLQNGPYPEMLKRRIRSKRGHMSNAAAAKCLQA
                     FGKDVPQVILAHLSETNNTPDRVIASARDGLGLFFEEKTVIIATQCGTSGSCPQCIKF
                     "
     gene            complement(375..1571)
                     /locus_tag="VFC43_04885"
     CDS             complement(375..1571)
                     /locus_tag="VFC43_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30554.1"
                     /translation="MDEECQSDAIPRVKLNQMKKDFFDYIEKNSEKIDSNVPVFYGHI
                     VTTLEKSIPTISDDLYDQFIDNITLRVLETSKFSSDISYVEKLFGYAERNKRKKTGQA
                     LYDIIVGIKMINSGKYDEAIEQLKKYRKIDAMICAAIAYCYLIRAKPPGGGGGPEDPH
                     RPPSQKELAAREMMIELVRLNPPVNRLREMGIGDDPGINMVFWFMLKQAIEWFPQDRE
                     YLRIGIEKASKDGRRDVREELLGLAIERFFDDMYFLRELYNLKLESRDAMGVAGIIKQ
                     MTQQYPDELEPIYYGLKLAIITSREEIYYKFRKMAISKNIPQRALVLLDFGFEIMSGK
                     HFEAHACLDEIKVTFGPHHHYIILLEFVAHDFLSEDEKKVKQAKRALIDSIDRYCHKL
                     LKIKDT"
     gene            1738..3390
                     /locus_tag="VFC43_04890"
     CDS             1738..3390
                     /locus_tag="VFC43_04890"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HZK30555.1"
                     /translation="MLFMDFSRACEQLEGTSGRLDMIDIISRILPTLSPEELPVFVRF
                     VMGQIFPDWSSKKLGIGPNLLYEAIGYVAGMKKEQVIEKINATGDAGRAVEDLLLSKS
                     QTTFFHEELDLVRVYTELITIADMEGKKSQREKLLAVRRLLGNSHPLEGRYLTRIMLE
                     ELRIGVGEGSVRDAIAKAFNVDSALVEHGMQALNDAGEVARLAKLGPDALRNVRITLF
                     HPVRMMLAQQGTIQDMIAEHGQIAAEFKYDGSRFQFHKKGNWARLYSRRLEDVTAALP
                     DVIEKLISSTSHDVILDGEVIAIKDNKPMPFQSVLRRFRRRHDIAEAQDAVELVPNVF
                     DILYLDEETLIDLPLDERRKRLESAVTMYIAPQVVSSDSREIERTYKAALDAGHEGIM
                     IKVPASPYSPGQRGKQWIKIKPEVDTLDLAVIGGEWGEGKRAHVFGSFLVACQDAGKL
                     IPLSRVATGFSDEQLSEVYELLKDRVVKKAGKEVSFEPELVFEVGYAELQVSPTYEGG
                     FALRFPRFVRIRDDKDIADVETLDSIRQRYEHQTKSAQAYKV"
     gene            3467..4162
                     /locus_tag="VFC43_04895"
     CDS             3467..4162
                     /locus_tag="VFC43_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30556.1"
                     /translation="MIQKSGLTLILIIMVAGCVTPANTPEKPIGKGYVNLCISDPARA
                     DYWQNTDDTLWSSPGNSGQGNDRKAVAGFSSCDNFSVTIWEYALNDAVEWIHVTIHTK
                     DGRSPDGWLVADHVMQTSDETGTEWSDNYYSLAGRWDQTQRGNGAKIWYDFKTDRTFT
                     FNYDMMGNREDIQDKGSWAYLGNKTYILISNISGGHEPVYLTLYNDGKSFNSGIQYSS
                     DLAVGRELVYVKE"
     gene            complement(4388..5533)
                     /locus_tag="VFC43_04900"
     CDS             complement(4388..5533)
                     /locus_tag="VFC43_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30557.1"
                     /translation="MMRCAECKGKGLCGLPRCPVISRFHAQVSVERTSSYQGTSPSVF
                     IGSSGWPDMHGGPLLINDSDSPPEWLARNLGMEDIVGIRARTIRGRSSLQRVADNLQE
                     IALSTIPREVDVTFEKPVSFSLDFDGTVAPVGLNGVVHTMEVLGNARIERVVDRITSD
                     TDIGATDACMALRESEIDVYRISTLMTAGLLGKRRTFVPTRWAITAVDDTISRQLKSE
                     IARFSPVEEIRLFTAEIFGNRIVCILVPGDWKYEMIEIWGRHTLWGGEEDVIVQDREG
                     MTKQGYSPISGAYYSARLATCEYLKNIQRSARVIVIRNISGEYWAPLGTWVIREAARR
                     AMANPAVVCDSLPAAVKKASDIIGSDGWVSHSSLIPELRTQRTLFHF"
     gene            complement(5530..6180)
                     /locus_tag="VFC43_04905"
     CDS             complement(5530..6180)
                     /locus_tag="VFC43_04905"
                     /inference="COORDINATES: protein motif:HMM:NF021416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2117 domain-containing protein"
                     /protein_id="HZK30558.1"
                     /translation="MSLRSTQDRHENYRKIQGCIPGEPVFVNGIIIGLATSDTVVLTG
                     KGGRIVLRSGLEPKTHGLEKLALCHPVDLATAWCKSGPVRSASPLISGRAPGAGRVLV
                     IDHCGHEIYARMPDDCCGVLAIGDDTTGVCGHILAHRGIPVMGIVDGDRDVIVPPAFA
                     PGSVVAEVLGECDDDVGREIAKKVPEIQVIWDEWVKEILEYLGMRVHVVVDLRTKT"
BASE COUNT         1554 a         1575 c         1558 g         1504 t
ORIGIN      
        1 gcgatcgtgc tcgaaagcaa ccactgcccg gaaatgctgc agaacgggcc atatcctgaa
       61 atgctcaaac ggaggatccg ttcgaaacgc gggcacatgt ccaatgctgc cgctgcaaag
      121 tgcctgcagg cctttggcaa agatgtgccc caggtaatcc tggcccattt aagcgagacc
      181 aataacacac ctgatcgggt gattgcaagc gcccgtgacg ggctcgggct cttttttgaa
      241 gaaaaaaccg taattatcgc cacacaatgc gggacttccg gttcctgccc gcagtgtata
      301 aaattttaat aaaagttccg tattctgcgt aacccgggag atgatttgtg cggagaaaac
      361 ggcttttttg tcgtttatgt atccttgatc ttcagcagtt tgtggcagta ccggtcaatc
      421 gagtcaatca gggccctttt tgcctgtttg actttctttt catcctcaga caggaaatcg
      481 tgcgctacga attcgagcag gattatgtaa tgatgatggg ggccaaaggt cacttttatt
      541 tcatccaggc aggcgtgtgc ctcaaagtgc ttgccactca ttatctcaaa tccgaaatca
      601 agaagaacaa gtgcacgttg cgggatgttt ttacttatcg ccattttcct gaacttgtag
      661 tagatctcct cgcgggaagt gattatggcc agtttaagcc cgtaatagat tggttcgagc
      721 tcatcgggat actgctgggt catctgcttg attatgccgg ccactcccat ggcgtcacgg
      781 ctctcgagtt tcagattgta aagttccctc aggaaataca tgtcatcgaa aaagcgttca
      841 atcgcaagcc cgaggagttc ttcccgcaca tcgcgcctcc catcctttga cgctttttcg
      901 atcccgatgc gtaaatactc ccggtcctgg ggaaaccact caatcgcctg tttgagcatg
      961 aaccagaaga ccatgtttat accgggatca tcgccaattc ccatctccct gagccggttc
     1021 accggagggt tgagtcggac cagctcgatc atcatctcgc gggctgcaag ttctttttgt
     1081 gaggggggcc ggtgaggatc ttccggacca ccgcctcctc ccgggggttt tgcacggata
     1141 agatagcagt acgcgatagc cgcacagatc atggcatcga ttttccggta tttttttaac
     1201 tgctcaatgg cttcgtcata tttgccactg ttgatcattt tgatgccgac gatgatatcg
     1261 taaagagcct gtccggtttt tttgcgcttg tttcgttcag cataaccgaa cagtttctca
     1321 acataggaga tatccgaaga aaatttgctc gtttcgagta cgcggagcgt gatattgtcg
     1381 atgaattggt cgtagaggtc atcgctgata gtgggaattg atttttcgag tgtcgtaacg
     1441 atatgcccgt aaaaaacagg gacattggaa tcgatttttt ctgaattttt ttcaatataa
     1501 tcgaaaaaat cctttttcat ctggttgagt tttactctgg ggattgcatc agactggcac
     1561 tcctcgtcca tactaccaga tcactgttgc gggtaataaa tttgttgaca tttttgtcag
     1621 gtgaacacaa aaaccgtgat ttctttttaa aaatccccca atattcagcc atttcaagat
     1681 tactgtataa cacaatgtta atgcgtgcgt ctggataaca ttccacacga gaaactcatg
     1741 ctttttatgg acttttcccg ggcctgcgaa cagctggaag ggacttcggg gcgtcttgac
     1801 atgatcgata ttatcagtcg tattcttccc accctgtcac cagaagagct cccggttttt
     1861 gtccggtttg tcatgggaca gattttcccg gactggagct caaagaaact cggtatcgga
     1921 cccaacctcc tgtatgaagc aatcgggtat gtggcgggta tgaaaaaaga gcaggtgatc
     1981 gagaagatca atgcgaccgg cgatgccggg cgggctgttg aggacctgct cctgagtaaa
     2041 tcccagacaa cctttttcca tgaggaactg gacctcgtcc gcgtctatac tgaacttatc
     2101 acgattgcgg atatggaggg gaaaaaatca cagcgtgaaa aactcctggc agtacgccgg
     2161 ttgctgggca attcccaccc gcttgaagga cgatatctta cccggatcat gctcgaagaa
     2221 ctccgcattg gggtcggaga aggcagtgtg agggatgcta tcgccaaagc cttcaatgtc
     2281 gactctgccc ttgtagagca tggaatgcag gcattaaacg atgcgggtga ggttgcacgc
     2341 ctcgccaaac tcggccctga tgcactccgg aatgtccgta ttaccctgtt ccacccggtt
     2401 cggatgatgc tcgcccagca gggaacaatc caggacatga ttgctgagca cgggcagatc
     2461 gcagccgagt tcaaatatga cggttcccgg ttccagtttc ataaaaaagg aaactgggca
     2521 aggctatatt cccgcaggct cgaagacgta accgctgccc ttccggatgt gattgaaaaa
     2581 ctgatctctt caacctcgca tgatgtaatc ctcgatggtg aagtgatcgc cataaaagac
     2641 aacaaaccca tgccattcca gtcggtcctg aggcgtttcc ggcgacggca tgatatcgca
     2701 gaagcacagg atgcagtcga gttggtaccc aatgtatttg atatcctcta ccttgacgaa
     2761 gaaaccctta tcgacctccc gttagatgaa cgaaggaaaa ggctcgagag tgcggtaacc
     2821 atgtatatcg ctccgcaggt cgtaagcagc gattcgcggg aaattgaacg gacctacaag
     2881 gcagccctcg atgcagggca cgaagggatc atgatcaagg tacctgcttc cccctattct
     2941 cccggccagc ggggcaaaca atggatcaag attaaacccg aggtggatac tctcgacctg
     3001 gcggtaattg ggggggaatg gggggaaggg aagcgggcac atgtctttgg ttcattcctt
     3061 gttgcctgcc aggacgcagg aaaactcatc cccctcagcc gggttgcgac gggtttttct
     3121 gacgaacagc tttcagaggt ctatgaactg ttaaaagacc gggtcgtgaa aaaggccggt
     3181 aaagaagtat cgtttgagcc tgaattagtg tttgaagtgg ggtatgccga gctccaggta
     3241 agcccgacct acgagggggg gtttgccctg cgtttcccga gatttgtccg gatcagggac
     3301 gacaaggata tcgcagatgt tgaaacgctc gacagtatcc ggcagcggta cgaacatcag
     3361 acaaaatcag cccaggcgta taaagtttag aaaaaggatt aaatatatag gaaaaaggaa
     3421 agccggttac acaaactcaa ttcaatctac cggtccttat gaaattatga tccaaaagtc
     3481 aggtttaacg ctgattctga ttatcatggt tgcggggtgc gtgactcccg caaatacacc
     3541 agaaaagccg atcggaaaag gatatgtgaa cctgtgcatt tctgacccgg cacgggcaga
     3601 ttactggcaa aataccgatg ataccctgtg gagcagtcct ggaaacagcg gtcagggaaa
     3661 tgaccgtaaa gcagtcgcag gtttttcctc gtgtgataat ttttctgtca cgatctggga
     3721 atacgcactg aatgacgctg tggaatggat tcatgtcacg attcatacaa aagatggaag
     3781 atctcccgat gggtggctgg ttgccgacca cgtaatgcaa acatctgacg aaacaggaac
     3841 tgaatggagt gataactatt attcgctggc tggtcgctgg gaccagacac aacgaggaaa
     3901 cggagcaaaa atctggtatg attttaaaac agacaggaca ttcaccttca attacgatat
     3961 gatgggaaac agggaagata ttcaggataa gggcagctgg gcatatcttg gaaataaaac
     4021 ctatattctg atttctaata tatccggagg tcacgaaccg gtttacctaa cactttacaa
     4081 tgatggcaaa tcatttaatt ccggaatcca atactcgtca gatttggcag tcgggcggga
     4141 acttgtttat gtaaaggagt gatatccctt ccttatgtat tcccgagcct cattattccc
     4201 ggcaaattga tggcgagaca tatcttaaat gtgcgttaaa aaaattacca tgctgacccc
     4261 gggaagacta acccttggga ctccctatac acagacacgt tcccaaaccg ggttcagatg
     4321 attggacttt caaccacgtt gagcagcatt tcctgatgca ggaaccaggg gataaccgcc
     4381 gaaccgttca gaaatgaaag agcgtccgct gtgtgcggag ttcggggatc agggagctat
     4441 gcgataccca cccgtctgat cctataatat ccgatgcctt tttgaccgct gccgggagcg
     4501 aatcacagac caccgccggg tttgccattg ccctccttgc cgcctcacgg atcacccacg
     4561 taccgagtgg cgcccagtac tcacccgaga tgttccggat tacaataacc cgtgctgacc
     4621 gctgtatgtt ctttaagtat tcacaggtcg ccagccgggc agaatagtaa gcgccggata
     4681 tgggggaata tccctgtttt gtcatacctt cccggtcctg gacaatcaca tcctcttccc
     4741 cgccccagag ggtatggcgc ccccagatct ctatcatctc atacttccag tcgccgggga
     4801 caaggataca gacaatcctg ttcccgaaaa tctctgcggt gaaaaggcgg atctcttcaa
     4861 cgggggagaa acgggcgatc tctgatttca gctggcggga tatcgtatcg tctacagcag
     4921 tgatagccca ccgtgttggt acgaacgtcc gccgtttgcc aagcaggccg gcggtcataa
     4981 gtgttgagat ccggtacaca tcgatttctg attccctgag cgccatacac gcatcggtag
     5041 cgccgatatc ggtatcagag gttatccggt caacaaccct ttcgatccgg gcattcccaa
     5101 gaacctccat ggtatgcacc accccgttta atccgaccgg tgcaactgtc ccgtcaaaat
     5161 caaggctgaa acttacgggt ttttcaaacg tgacgtccac ttcccgcgga attgtggaga
     5221 gggcgatttc ctgcaggttg tcagcaactc tctgcagtga tgaccgcccc cgtatggtcc
     5281 gggcacggat accgacgata tcttccatcc cgagattgcg ggcaagccat tccgggggac
     5341 tatcgctgtc attgatgagg agcggaccgc catgcatatc cggccagccg gagctcccga
     5401 taaaaacaga gggggaggtg ccctggtaac tgctcgtgcg ttccacagag acctgggcat
     5461 ggaaccggct tataaccggg cagcggggca gaccgcacag cccttttccc ttacactcag
     5521 cgcatcgcat catgtttttg ttctcagatc cacgaccacg tgcacccgca tgccgagata
     5581 ctcaaggatc tcctttaccc actcatccca gataacctgg atttccggta ctttttttgc
     5641 aatctcccgc ccgacatcat catcgcattc tcccaggact tctgcaacta ccgagccagg
     5701 agcaaatgcc ggcgggacga ttacatcccg gtcgccatcg acaatcccca ttacaggaat
     5761 accacggtga gcaaggatat gcccgcagac ccccgtcgta tcatcaccaa tggcaagcac
     5821 gccgcagcag tcatccggca tccttgcata gatctcatgt ccgcaatgat cgatgacgag
     5881 caccctcccg gcaccgggcg ccctgccgga aatgaggggt gaagcggatc tgacaggccc
     5941 gctcttgcac catgcagtag cgagatcaac aggatggcac agggcaagtt tttcaagacc
     6001 atgggttttt ggttccagtc cgctgcggag aacaatccgc cctcctttac ctgtaagaac
     6061 gactgtatcg gatgttgcaa gaccgatgat aatgccattc acaaaaactg gttctccggg
     6121 gatgcagcct tgtatcttac gataattttc atgtcggtcc tgagtgctcc gtaatgacag
     6181 ttccggatac c
//
LOCUS       DASRLV010000124        11992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_446073,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000124 DASRLV010000000
VERSION     DASRLV010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 11992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11992
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446073"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1506
                     /locus_tag="VFC43_04910"
     CDS             <1..1506
                     /locus_tag="VFC43_04910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZK30559.1"
                     /translation="KKAELRVEESERQFRLLAENSLDMIGRIKPDFTHMYASPAYTTT
                     LGYFPEEVIGKPGRLFIHPDDVHIMESARTILTEQNSSGTLRFRSKHKDGHYVWVESH
                     VRSIFDEKTHDVSEYYTVTRDISEQKTAEEALQESEDRYRKLVEISPDAVFLHRNGKI
                     IYANPATFTLLGATRPDEIIGKSVLDFVSPEFKDIVRENIQKDLQAKVSPRTELHMVR
                     IDGTPIIIEGRGIATEINGKPAVQVAIRDITEQNRAEEELCKSQGLYHNLAESSSDLI
                     FVIGRDDRVEYANSFASSMVTKPMNQIIGHPRSSLFPAEASRTQKIALETVFETGSSV
                     RNTGPLTVNGQLRWFDHILIPLKDTGGKIQSVLGISRDITDRKNTEETLIHNEQRFRN
                     LINAVGDIVWEIDAEARFVYVSPQVEAILGYTPDELIGHTPFEFLHPDTIPQNHKKFR
                     NASEGQVKSIIHESQWIHKDGHMVTIESNAIKLFDSNGSFSGFIGIDRKRP"
     gene            complement(1572..2390)
                     /gene="dapF"
                     /locus_tag="VFC43_04915"
     CDS             complement(1572..2390)
                     /gene="dapF"
                     /locus_tag="VFC43_04915"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HZK30560.1"
                     /translation="MEIPFTKLHGNGNDFVLIDETEKIIIPDDMKGQFAALYCDRRFG
                     IGGDGVIFLSKSEKSTLKMRILQPDESEAEMCGNGIRCLAKYAFDTGYAKEFCTVETP
                     AGEMSVAMGYKEDEFSATITMTTPKFDRKEIPATGEGEYKERIGDFDVYAVNTGVPHA
                     VIIVDAVDAIDVETAAPAIRRHASFEKGANVNFVERTGPDSIRIRTFERGVEEETLSC
                     GTGATASAAIVHKLGYAGDTVSVETRGGPLTIYLKDGAKMEGTATTVFSGKISY"
     gene            complement(2441..3403)
                     /locus_tag="VFC43_04920"
     CDS             complement(2441..3403)
                     /locus_tag="VFC43_04920"
                     /EC_number="5.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0046033 - AMP metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphate isomerase"
                     /protein_id="HZK30561.1"
                     /translation="MFVTETAKKIKSMEIRGAGRIAREAAEALRHHATEIRAGDLRSF
                     QREMDSAAQTLIATRPTAVSLPNAVHIVMSGLERAETLEEAKKGVLARAEQFIYSSRH
                     AVENIAQFGSRHIRDGDTLLTHCNSEAALGCIIEAHRSGKEIEVFAIEVRPRNQGHFT
                     IRTLNDAGIKTNFIVDSAVRSFINEVDLVIVGADAVTVNGAVVNKIGTSMVAHTAHEA
                     RVNVLVAAETYKFAPRTIFGELIQIEERDGDEVLPAEIAKTLPNVTVRNPAFDVTPAE
                     YIDLIVTEAGAIPPQMAYIIIREYLGWGIEEFHKTFEINTIHEE"
     gene            3461..4276
                     /locus_tag="VFC43_04925"
     CDS             3461..4276
                     /locus_tag="VFC43_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HZK30562.1"
                     /translation="MSLAVVFDSAGTLLNTYRVAKDIGNKKLLPGIETTTLTFSSPDR
                     VLVVLPVHSKDIIKTPGEILLSSYLVEHDLGFGVSCTRKIVTAEEIGDILYTDQRVRV
                     SDLQECIRNVWTVCKAESIVTLNSGAIVNMAYKCIEFAITAGGWPFDGAKETINALHR
                     MGVPVFIASGDRVTKLEKMADHLGIPRDRVYGVATPTVKAQIINDLRQDYDKVLMVGD
                     GINDLCAMQNADIAILTIQQAADRPEELYHEADFVVNNVREVVPIVQKSLASK"
     gene            4354..5967
                     /gene="atwA"
                     /locus_tag="VFC43_04930"
     CDS             4354..5967
                     /gene="atwA"
                     /locus_tag="VFC43_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl coenzyme M reductase system, component
                     A2"
                     /protein_id="HZK30563.1"
                     /translation="MKAPLITVDNLCMDFDSTRVLKNISFEIAEGEILGIIGRSGAGK
                     TVLMHLIRGVEQPPTGGRIIYHVAACNGCDYMDVGSAAGKPCPHCSGTLLALDVDLWC
                     EKDETLKRRLMRRTAIMFQRTFALYGDDRVIENVLHALDDIDYPQDNAINRAADLIDQ
                     VRLSHRMMHIARDLSGGEKQRVVLARQLAKEPFLLFADEPTGTLDPGTAKIVHAMLID
                     AAKTNNMGMVVTSHFSQVIEDVAHRAILLVDGGIAKIGTPKEVIAEFMKGYDDTEKFE
                     DTELGEKVVVARDLFKRYISVDRGVVKAVNGVTFEVFAKEIFGIIGKSGAGKTTLSGI
                     IAGLVEPTSGEMNIRIGEEWVDMTKPGIEQRGRATAYIGLLHQEYDLFPHRTILDNLT
                     DAIGLEFPKELAMRKALVTLKMAGFSDEKSREILERMPGQLSEGERHRVALAQVLIRE
                     PRIVILDEPTGTMDPITKIDVKHSIMHSREEMEETFIVVSHDMEFVRDVCDRLALMRG
                     GKIVQIGKTAEVLAFLTDEERKLMSQPSA"
     gene            5976..7505
                     /locus_tag="VFC43_04935"
     CDS             5976..7505
                     /locus_tag="VFC43_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 3 protein"
                     /protein_id="HZK30564.1"
                     /translation="MITVHLDGERLEIIEGSTLGSIIPNHSPECSIAVIRPSTKEQSK
                     TGNLAISTTAGEVTIEIARGSEDFLKSPDIVQKLALHWSDRYATAFGPFPSTIHPMRT
                     PHIYERGDVILGCGGYEPARSYLIFSKVHHSADHGADERGGVFAKVVSGRGVLDRWTP
                     GDRITKIEPVISWADTSRSFTTTDLGLVIEDGTQIATRVNVIAQGYNPDRITTEAAGS
                     VEHMLLGLKTGRFHVSRVTSTHILDSGLAGTSDIEREFRRPRREGTVTVRTSGQLAGG
                     IYIYRDDVPSSPVHDVVGQVVHGIELVKLAKEHDILMVSVMPQRIDLLGLPLKKAKAI
                     AAAERISLHIDNDEGERIVVSQDPGNTLDVLKDRSVTVTTAPLDKVIDITLDDERAPV
                     SCDIFRRITGLKQHDAGMIPVFFKFDDVVLFRSAVPAGSKINPENIPTGEVPAAALAI
                     TNDSRKASGFVGVRLSANKEFGPTSEPFEGTNILGSVIDTEKLKKVKEKENVYIREVK
                     R"
     gene            7502..7960
                     /locus_tag="VFC43_04940"
     CDS             7502..7960
                     /locus_tag="VFC43_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012108013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 6 protein"
                     /protein_id="HZK30565.1"
                     /translation="MTEYTPTHAGTVTKYMVVESFEITPADLALRAYEISQGVMIKET
                     CFGIVINGNEKEVDHIIAELRKLDPEHIFVKDRGFPPGDPRRCRANLGGARPGYNGME
                     HEMTIIPYVSHGLEELKKYDEKTIPTPENPLEHPFLDIYKLKKLIDAQEP"
     gene            7962..8408
                     /locus_tag="VFC43_04945"
     CDS             7962..8408
                     /locus_tag="VFC43_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 5 protein"
                     /protein_id="HZK30566.1"
                     /translation="MAKVFIYPSTSLILSDMVSRFGHTPLSSAIAIRERIQTAGLESP
                     PLQITPEEPKKGLKWAAVEVPAGVRGRMSLYGPMIGECEAAIIINDADLAFGCMGCAR
                     TNELIKFLLHQKDIPKLELNYPKNEEEGVKFVAAIKRFLTDLGVPK"
     gene            8405..9643
                     /locus_tag="VFC43_04950"
     CDS             8405..9643
                     /locus_tag="VFC43_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 15 protein"
                     /protein_id="HZK30567.1"
                     /translation="MTHPVRIAQLSCGPEYSGIQHEIDTAAREVGGEIFYPDVALKDI
                     RRDFDKFGLDVRSPDLKLAIARAMALVEGRVEADAVFIATCFRCAEAAIVRNELRRYI
                     NEHSKLPVVSYSFTERTTAGTLLTRMEALTTIARRRALLARETQSGLTLGLDSGSATT
                     KAVVMRDNKIIGTGWQPTTEVIKSAQEVIADALHNAGVTRDEIQAVGTTGYGRFLVGK
                     EIHADLIQEELTVNSKGAVYLANRQHGPATVIDIGGMDNKAISVMDGIPGVFTMGGIC
                     AGASGRFLEMTAKRLGVDITELGPLSMKGFGGRVPMNSYCIVFGTQSLVNALAAGSTR
                     EDVAAAACHSVAEQVFEQQLQEVDIKEPVIMVGGTSLIEGLVFAMGELLQTKIVVPPY
                     SQFIGAVGSALLVSGFIKDD"
     gene            9644..10225
                     /locus_tag="VFC43_04955"
     CDS             9644..10225
                     /locus_tag="VFC43_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012108016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 17 protein"
                     /protein_id="HZK30568.1"
                     /translation="MLAIEYFEVECPEPVGAEYYKQITNDVLLDHNLLRVIHKLHIYI
                     DPKVPVFIAVGVLKKLTTRVRVGDFANVNPLEGKITLVISDETYLAPMLKIFWQKFGK
                     DHVEQPDRFTVILYDVKADLKEIEEIVVADPSESLYKDLIYALRCIAPEGFRLRSEKF
                     SKEKFSFVASENTLAENVDDIVREKFALMGEVP"
     gene            10222..11160
                     /locus_tag="VFC43_04960"
     CDS             10222..11160
                     /locus_tag="VFC43_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 7 protein"
                     /protein_id="HZK30569.1"
                     /translation="MTLVPITYKGGVYQHDIVIDLIEDLGGYIIQKHVIAAEVVLQCF
                     VPREDIELIRTIAKPLFGEVTDTPLVGTEIAIVSMSLEIHHLPHPSCDIAEYVRRIGA
                     KSNMVSLARGPGKRIAGLNDEERDVINEHDIAVYLMGNFETCIEYKMPTLRRGIEVPI
                     VLCGGPDKETLQKIIDPPVDGYVGNVGRFMRRTKEADEIEKLDEIIAEITRVLEKKRE
                     EIQKDPLSVSPARLMGLINEKVPAIHGVTSPTPLTVQMAGLRVKLPYDTFAAELRKLE
                     IEDGILIGDIADVVPSRMRDYILVKILPFSETNTVI"
     gene            11160..11645
                     /locus_tag="VFC43_04965"
     CDS             11160..11645
                     /locus_tag="VFC43_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZK30570.1"
                     /translation="MEQTTKEEFEERLARIVARGADVTAEEWLREIEVEYGRVPLIFR
                     RMGERPEVLISHLLYKGTVAQTSPLDPKYVELISLAVGAALKCPHCTGYHMQAALKMG
                     ASREEILEVILLAGMISNSSVLANSYRIFDEKMEQCIPCQSKGVNVPGSKRKPAAIKK
                     K"
     gene            11788..>11992
                     /locus_tag="VFC43_04970"
     CDS             11788..>11992
                     /locus_tag="VFC43_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012108020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZK30571.1"
                     /translation="MKSIILAGGVGTRLWPLSRQYYPKQFIPLDGQSLFQKTYERATR
                     LSDPEEIFVVTNEIHQYLVRNQIE"
BASE COUNT         3194 a         2859 c         3035 g         2904 t
ORIGIN      
        1 aagaaggccg aactgagagt ggaggagagc gaacgacaat tccggttgct ggcggaaaac
       61 tccctcgata tgattggccg gatcaaaccg gattttaccc atatgtacgc ttcacccgcc
      121 tacacaacca cgcttggcta ctttcctgaa gaggtgattg gtaaaccggg acggctcttt
      181 atccacccag atgatgtcca tataatggaa tccgccagga ctattctgac agaacaaaat
      241 tcttctggaa cgttaagatt ccggtcaaaa cataaagacg gtcattatgt ttgggtagaa
      301 tctcatgtca ggtctatctt tgacgaaaag acccatgatg tatcagaata ttacaccgtc
      361 acacgggata tttccgaaca aaagaccgca gaggaggcat tgcaggaaag cgaggaccgg
      421 taccgcaaac ttgtagaaat atctcccgac gctgttttcc ttcaccggaa tggaaagatt
      481 atttatgcaa atccagccac cttcacactt ctgggtgcta cgcgtccgga tgaaattatc
      541 ggaaaaagtg ttcttgattt tgtttccccg gaattcaagg atattgtcag ggaaaatatc
      601 caaaaagatc ttcaggcaaa ggtatcgcca cgaactgaat tacacatggt tcgcattgac
      661 gggacaccaa taataattga aggtagggga attgcgacag aaatcaatgg aaaacccgct
      721 gtgcaggtcg caatacgaga tataaccgag caaaatcggg cggaagagga attgtgcaag
      781 agtcagggac tctatcacaa ccttgccgag agttccagtg atctgatatt tgttattggc
      841 agggatgacc gggtagaata tgcaaacagt ttcgcctcgt cgatggtcac taaaccgatg
      901 aaccagatta ttggtcatcc acgttcctcc ctttttccgg ctgaagcgtc cagaacccag
      961 aaaatagcac tcgaaactgt ctttgaaaca ggctcttccg tacgcaatac gggacccctc
     1021 acggtaaatg gtcagttacg ctggtttgat cacatcctga taccattgaa agacaccgga
     1081 gggaagatcc agtcagtcct cgggatatcg cgggacatta cagatcgaaa aaatactgag
     1141 gaaacgctca tccataacga acagcgattc cgaaatctca tcaatgctgt tggggatatt
     1201 gtctgggaaa ttgatgcaga ggcacgcttt gtatacgtga gcccgcaggt tgaagcaatc
     1261 cttggctata caccagatga actgatcgga cataccccct ttgaatttct ccatccggat
     1321 acaatcccgc aaaaccataa aaagttcagg aatgcgtcag aaggacaggt gaaatctatc
     1381 atccatgaat cccaatggat tcataaagat ggccatatgg taactatcga gagtaacgcg
     1441 atcaaactgt ttgacagtaa cggttcgttc tcgggcttta tcgggatcga tcgtaaacga
     1501 ccgtgatgat gtgttcagat aagggtttgt tgacttctgc ctcatgataa aaagaatgcc
     1561 aaaaagaggt tttagtatga aatttttccg gaaaaaactg ttgttgccgt gccttccatt
     1621 tttgcaccgt ccttgagata aatggtaaga ggaccacccc gggtttccac gctgacagta
     1681 tctcctgcat atcccagttt gtgcacaata gcagcagacg ctgtggcacc ggttccacag
     1741 gaaagagttt cttcctccac accgcgctca aacgtacgga tcctgatgct atcgggtcct
     1801 gttctttcaa cgaaattcac gttcgcccct ttttcgaatg atgcatggcg gcggattgcc
     1861 ggagctgctg tttcaacatc gattgcatca acagcatcaa cgataataac tgcatggggg
     1921 acgccggtat ttaccgcata gacatcgaaa tcccctatcc tttccttgta ttcgccttcc
     1981 ccggttgctg gtatttcttt ccggtcaaat ttcggtgtag tcatggtgat cgttgcagaa
     2041 aattcgtcct ccttataccc catagctacg ctcatctctc ctgcaggtgt ttcaacagta
     2101 caaaattcct ttgcataacc ggtgtcaaaa gcatatttgg caaggcagcg gatgccgtta
     2161 ccacacatct ctgcttcgct ctcatccggc tggagaatcc gcattttcag cgtacttttt
     2221 tccgatttgg agaggaaaat aaccccatcc cccccaatgc cgaaccgacg atcacaatag
     2281 agagctgcaa actgcccttt catgtcatcc ggtatgataa ttttttcagt ttcgtcaatt
     2341 aatacaaaat cattgccgtt tccgtgtaat ttggtaaatg gtatttccat gaaattatcc
     2401 ctcagttact ttccctgtca agcgtaaagc cttcatacac tcattcctcg tgtattgtat
     2461 taatttcaaa ggttttatgg aattcttcga taccccatcc aagatattcc cggatgatta
     2521 tataggccat ttgtggggga atggcacccg cctctgtaac aataagatcg atatactctg
     2581 cgggagtcac gtcgaatgct ggatttcgca cggtgacatt cgggagagtc ttggcgatct
     2641 ccgccggcag tacctcatct ccatcacgct cctcaatctg gattaattca ccaaaaattg
     2701 tccgtggcgc aaacttgtag gtctctgctg caacaaggac attcacccgt gcttcatgcg
     2761 cagtatgggc aaccattgat gtaccaatct tgttcaccac cgccccgttt actgtgactg
     2821 catctgcccc cacgatcaca agatcaactt cattgatgaa tgatcggacg gcggaatcaa
     2881 caataaaatt ggttttaatc ccggcatcat tgagtgtcct gatcgtaaaa tgtccctggt
     2941 ttcgcggacg tacttcgatt gcaaaaacct caatctcctt accgctccgg tgcgcttcaa
     3001 tgatgcaccc gagtgcagct tccgaattgc aatgcgtgag aagagtgtcc ccgtcccgta
     3061 tatgtcggga accgaactgt gcgatgtttt ccacagcgtg cctggaggag taaatgaact
     3121 gctccgcacg tgcaagcaca ccttttttcg cctcttccag tgtttctgcc cgctcaagcc
     3181 cggacataac aatatgtaca gcattcggga gagaaaccgc cgtaggtcgg gttgcgatca
     3241 gggtctgtgc agcagaatcc atctcacgct ggaaagaccg aagatcgccg gccctgattt
     3301 ctgttgcatg atgccggagt gcttcggcag cttcacgcgc aattctgcca gcaccccgta
     3361 tctccatgct ctttatcttc tttgcggttt cagttacgaa catcatcttc aatgattcaa
     3421 tgttcagaaa agtacataga gtttatcacc gggaattacc atgtcgttag cggttgtttt
     3481 tgatagtgcg ggaacactcc tgaacacgta ccgggttgca aaggacatag gcaacaaaaa
     3541 gctcctgccc ggtattgaaa ctacaacact cacgtttagt tcacctgatc gcgtcttagt
     3601 tgttttgccg gtccactcaa aagacattat aaaaacgccg ggagaaatat tgctttcgtc
     3661 gtacctagtt gaacatgacc tgggattcgg ggtgagctgt acaagaaaaa ttgttaccgc
     3721 tgaagaaata ggcgatattc tctacacaga ccagcgggtc cgtgtgagtg atcttcagga
     3781 atgtatccgg aacgtctgga cagtttgtaa agcggaatcc attgttaccc tgaacagcgg
     3841 tgcaattgtc aacatggctt acaagtgtat agaatttgca ataactgccg gtggctggcc
     3901 atttgatggt gctaaagaga ccataaacgc actccaccgc atgggggttc cggtattcat
     3961 cgcatccggt gacagggtaa cgaaactgga aaagatggca gaccacctgg ggattccccg
     4021 cgaccgggtt tatggagttg caacccccac ggtaaaagcg cagatcatca atgacctgag
     4081 acaggattac gacaaggtac tgatggttgg cgatggaatc aatgacttat gcgccatgca
     4141 gaacgccgat attgcaatac ttacgataca acaggccgca gaccggccgg aagaactcta
     4201 tcacgaagct gatttcgtgg taaacaatgt acgtgaagta gttccgatcg tgcagaagag
     4261 tcttgcatca aaataacatt tcgatcgtga cgactgtaat gcagcatata aaaggtaata
     4321 tgccacaagt atcacattag gtataagggt attatgaagg ctccactgat cacggtcgac
     4381 aacctctgca tggattttga tagcacgagg gtcctcaaga acatttcttt tgagattgcc
     4441 gaaggggaga tcctgggaat tattggcagg agtggcgcgg gtaagacggt tcttatgcac
     4501 ctcatacggg gtgtcgagca acccccgaca ggaggccgga ttatttacca tgtagccgca
     4561 tgtaacggat gcgactatat ggatgtcgga agtgccgcag ggaaaccctg ccctcattgc
     4621 agtggcacac ttttggcact cgatgtagat ctctggtgtg aaaaggatga gacactgaaa
     4681 cgccgtctga tgcgacgtac tgcaatcatg ttccagcgaa ccttcgcgtt atatggtgat
     4741 gaccgtgtca ttgaaaatgt acttcacgct ctggatgaca ttgattatcc tcaggataac
     4801 gcaatcaacc gggcagcgga cctcatagat caggtaagac tttcacacag gatgatgcat
     4861 atcgcccgcg atctttcagg tggggagaaa cagagagtgg ttctggcacg tcagctggca
     4921 aaagaacctt tcctgctttt tgccgatgaa cctaccggca cactcgatcc cggcactgca
     4981 aaaatagttc atgcaatgct gatagacgcg gcaaagacaa ataacatggg aatggttgtc
     5041 acctcccact tctcccaggt cattgaagat gtggcccatc gcgcaatcct ccttgttgat
     5101 ggcgggattg caaaaatcgg aacacccaag gaagtcatcg cggagttcat gaaaggatat
     5161 gacgacaccg aaaaatttga agatactgag cttggcgaaa aggtcgttgt tgcccgggac
     5221 cttttcaaac gctatatctc tgtcgatcgc ggtgtcgtta aagcagtaaa cggggttact
     5281 tttgaggtat ttgcgaaaga gatttttggt atcatcggta agagcggagc aggaaagacc
     5341 actctttcag gaattattgc cggtcttgtc gagcctacca gtggagagat gaatatcagg
     5401 attggcgaag aatgggtcga tatgaccaaa cccggtattg agcagcgcgg cagggcgacc
     5461 gcgtatatcg gtctcctcca tcaggagtac gaccttttcc cccaccgaac gatacttgac
     5521 aacctcaccg atgcaatcgg gttggagttc ccaaaagaac tcgcaatgcg aaaggcactt
     5581 gtaacgctca agatggctgg attttccgat gaaaaaagca gggaaatcct ggaacggatg
     5641 cccgggcaac tctctgaggg ggagcggcac cgcgtggcac tcgcccaggt ccttattcgt
     5701 gagccgagga ttgtaatact tgatgaaccg accgggacga tggatcccat cacaaagatc
     5761 gatgtgaagc attcaatcat gcactcacgc gaagagatgg aagagacatt tattgtcgtt
     5821 tcccatgata tggaatttgt ccgggatgtc tgtgaccggc tcgcactgat gcgaggcggg
     5881 aagatagtgc agattggcaa gactgctgaa gtgctcgcgt tcctcactga cgaggaacgc
     5941 aagctcatga gtcagccttc cgcttgagga gtgtaatgat tactgtccac ctcgatggcg
     6001 agaggctgga gattattgag ggaagcactc tgggttcaat cattcccaac cattcgcccg
     6061 aatgcagcat cgcagttatc cgtccttcaa cgaaggaaca gtcaaagaca ggaaatcttg
     6121 ctatcagtac aacagccggt gaagtcacca ttgagattgc cagggggagc gaggatttct
     6181 taaaatctcc tgatattgtg caaaagctcg cccttcactg gagtgatcgt tacgcaacgg
     6241 cgttcgggcc ttttccctca acgattcacc cgatgcgcac accccacatc tacgaacggg
     6301 gtgatgtaat ccttggctgt ggtggctacg aaccggcacg atcctacctg attttctcta
     6361 aagtccacca ttccgcagat cacggggcag atgaaagagg tggggtcttt gcgaaagtgg
     6421 taagtggcag aggggttctg gatcggtgga caccaggaga ccgtataaca aaaatcgaac
     6481 cggtcatcag ttgggcggac accagtcgtt ccttcaccac gaccgacctg ggccttgtta
     6541 tagaggacgg aacgcagatt gcaacccggg tcaatgttat cgctcagggg tataaccctg
     6601 atcggatcac aactgaagcc gcgggtagtg tggagcacat gcttctggga cttaagaccg
     6661 ggaggtttca tgtcagccgg gtaaccagca cccatattct cgattcgggg cttgccggta
     6721 cgtcagatat tgaaagagag ttccggcgtc cgcgcaggga ggggacggta acggtgagaa
     6781 catcgggaca actggcagga ggtatctata tttaccgtga tgatgttccc agcagtcctg
     6841 ttcatgatgt cgtcggtcag gttgttcatg ggatcgaact ggtcaaactt gccaaagaac
     6901 atgatattct catggtcagc gttatgccac aacgaatcga ccttcttggc cttcccctta
     6961 aaaaggcaaa ggcgattgcc gccgcagaga gaatttccct tcatattgat aatgacgagg
     7021 gggagagaat tgttgtttca caggatccgg gaaacacact ggacgttctc aaggacaggt
     7081 cggtcaccgt aacaacagca ccacttgata aggtcataga cataacactt gatgacgaaa
     7141 gagcccccgt cagctgtgat attttccgcc gaatcaccgg tctaaagcaa catgatgccg
     7201 ggatgatacc ggtctttttt aaattcgatg acgttgttct gttcaggtct gctgtcccgg
     7261 caggatcaaa aattaatcct gagaatatac ccaccggtga agttcctgca gcggcactcg
     7321 ctatcacaaa tgattcccgg aaagcttccg gatttgttgg ggttcgcctc tcggcaaata
     7381 aagagttcgg ccctacttca gaaccttttg aaggcacgaa cattcttggg agcgttattg
     7441 atactgaaaa attaaaaaaa gtaaaggaga aggagaacgt gtatatcagg gaggtaaagc
     7501 ggtgaccgag tatacgccga cacatgcagg caccgtaact aagtatatgg tagtcgagtc
     7561 ttttgagatt actcccgccg accttgcact ccgcgcttat gaaatttcgc agggggtaat
     7621 gatcaaggag acctgttttg ggattgtcat aaacggcaat gagaaagagg ttgaccacat
     7681 tattgccgaa ctgcggaagc tcgatcccga acatattttt gtgaaggacc ggggtttccc
     7741 tcccggagat ccccgccgct gccgggcgaa cctcggcggt gcgcgaccgg gatataatgg
     7801 aatggagcac gagatgacca tcatccccta tgtttcacat ggtcttgaag aactcaaaaa
     7861 atatgatgag aagactattc ccaccccgga aaatccgctg gagcacccgt tccttgatat
     7921 ctataaactc aagaaactga tcgatgcaca ggagccctga catggcaaaa gtattcatct
     7981 acccctcaac gagcctgatc ctctccgata tggtgtcacg atttggacac acacccctta
     8041 gttctgcgat cgccatccgt gaacgtatcc agactgcggg gctcgaatcc cctcccctcc
     8101 agattacacc tgaagagccc aaaaaaggac tgaagtgggc tgcggtcgaa gtaccagccg
     8161 gagttcgtgg tcgtatgtca ctgtatggac cgatgattgg ggaatgtgaa gcagcgatca
     8221 tcatcaacga tgcagacctt gcgttcggct gtatggggtg tgcccggacg aatgagttga
     8281 ttaaatttct tcttcatcag aaagatatcc ctaagcttga actgaattac ccgaagaatg
     8341 aggaggaggg cgtaaaattc gttgctgcta tcaaacgctt cctcaccgat ctgggagtgc
     8401 cgaaatgaca catcctgttc gcattgcaca actatcctgc gggcctgaat attccggcat
     8461 acagcatgag atagataccg cggcacggga agtcgggggc gagatttttt acccggatgt
     8521 tgccctcaaa gacatacgcc gcgattttga taaattcggg ctggatgtaa gaagcccgga
     8581 cctgaagctc gcaatcgcaa gggcaatggc acttgttgag gggcgggttg aggccgacgc
     8641 cgttttcatc gccacatgct tccggtgcgc agaggctgca atcgtgcgta acgaactccg
     8701 gcgctatatc aatgaacatt caaagctgcc ggtggtcagt tactcgttta cagaacgcac
     8761 gactgccgga actcttctca caaggatgga ggcactcacc acgattgcgc ggcggcgtgc
     8821 ccttctcgca agggagaccc agagcggtct cacgctcggc cttgactcgg gatctgccac
     8881 gacaaaagcg gttgtgatgc gggacaacaa gatcatcggc acgggttggc agcccaccac
     8941 ggaagtcata aaaagcgcac aggaagtaat tgccgatgct cttcataacg cgggtgtcac
     9001 ccgcgatgag atccaagccg tagggactac cgggtatggc cggttccttg tcggaaaaga
     9061 gattcatgcc gatctcatcc aggaagaact gacagtaaac tcaaaggggg cggtctacct
     9121 tgccaaccgg cagcacgggc ctgcaacagt aattgatatc ggagggatgg ataacaaggc
     9181 catctcggtg atggatggaa tcccgggggt tttcacgatg ggaggtatct gcgctggtgc
     9241 aagcgggagg ttcctggaga tgacggcaaa gcgccttggc gttgatatta ccgagctcgg
     9301 cccgctttcc atgaaaggat ttggcgggcg tgttcctatg aacagttatt gtatcgtttt
     9361 cgggacacag agccttgtaa atgcactcgc cgcaggcagt acccgcgagg atgtggctgc
     9421 cgctgcgtgt cacagtgttg ccgaacaggt ttttgaacaa caactgcagg aagttgatat
     9481 aaaagaaccg gtaatcatgg tcggtggcac atcactcatc gaggggcttg tttttgctat
     9541 gggtgaactg ctccagacga agattgtcgt acccccctat tcacaattta ttggtgctgt
     9601 cggatcggcg ctgcttgtat cagggtttat caaggacgat tagatgctag ccattgagta
     9661 ctttgaagtg gaatgtcccg aaccagtggg cgcggagtac tataagcaga tcacaaacga
     9721 cgtcctgctc gaccataacc tcctgagggt tattcataaa cttcacatct acatcgaccc
     9781 gaaggtgcct gtctttatcg cagtcggtgt cttaaaaaaa ctcaccacga gagtccgcgt
     9841 cggggatttt gccaatgtaa atcccctgga aggaaaaatt actctcgtta tttcagatga
     9901 aacgtacctt gctccgatgc tgaagatctt ctggcagaag ttcggcaaag accatgtgga
     9961 acaaccagac cgtttcacgg taatcctgta tgatgtcaag gcggacctca aagagattga
    10021 agagattgtt gttgccgatc cgagtgaatc gctttacaag gatcttatct atgcattgcg
    10081 atgtatcgca ccggaaggat tccgccttcg aagcgagaaa ttcagcaaag aaaaattctc
    10141 atttgttgcc agtgagaaca cccttgccga gaatgtcgat gatatcgtga gggaaaaatt
    10201 tgcgcttatg ggcgaggtac catgacactt gtacctatca cctacaaggg aggggtctac
    10261 cagcacgata tcgttataga tctcattgag gatcttggcg gttatatcat ccaaaaacat
    10321 gttatcgctg ccgaagtcgt gctccagtgc tttgttcccc gggaagatat cgaacttatc
    10381 cgcacgatcg ctaaacccct ttttggggaa gtaacggata ctccccttgt cggcacagag
    10441 atagcaatag tcagtatgag ccttgagatc catcacctcc cccacccgtc ctgtgatatc
    10501 gcggaatatg tacgacggat cggggccaag agcaacatgg tcagcctcgc acggggcccg
    10561 ggaaaaagga tcgccggttt aaacgatgag gagcgggatg tcatcaacga gcacgatatt
    10621 gcagtgtatc ttatgggaaa ttttgagacc tgtattgaat acaagatgcc cactctccga
    10681 agaggtatcg aagtgcctat cgtgctctgc ggaggtcctg acaaagaaac cctccaaaaa
    10741 attatagatc caccggttga tgggtatgtt ggcaacgttg gcaggttcat gcgcaggaca
    10801 aaagaagccg acgaaattga aaagctcgac gaaattattg ctgagatcac acgggtgctt
    10861 gaaaaaaagc gtgaggaaat ccagaaagac cccctctccg tttctcctgc gaggcttatg
    10921 ggtttaatta atgaaaaggt tcccgcaatc cacggcgtaa cttctccgac accccttacg
    10981 gtacagatgg ccgggctcag ggtgaagctg ccttatgata cgtttgccgc agaactcagg
    11041 aagcttgaga tagaggacgg gatactgatc ggggatatcg ctgacgttgt tccctcccgc
    11101 atgagagatt atatccttgt taaaatcctg ccattctcag agacaaacac ggtgatatga
    11161 tggaacaaac aaccaaagag gaatttgaag aacggcttgc gcggattgtg gcgcggggag
    11221 cagatgtcac tgcagaggaa tggttgcggg agattgaagt agagtacgga cgtgttcccc
    11281 ttatattcag acgaatggga gaacgccccg aagtgcttat ttcccatctt ctctacaagg
    11341 gcacggtcgc tcagacgagc ccgctcgatc caaagtatgt agaacttatc agcctcgctg
    11401 ttggagcggc actaaaatgc ccccactgta ccgggtatca catgcaggct gctcttaaga
    11461 tgggtgcaag cagggaagag atccttgagg tgatcctgct ggcagggatg atatcgaatt
    11521 cctccgtgct cgcgaactcg taccggattt tcgacgaaaa aatggagcag tgcattcctt
    11581 gtcaatcaaa aggggtgaac gtgcccggat caaaacgaaa acctgcggca attaaaaaga
    11641 aataataatt cgatcaaagg cgggtattgc gggtgttttg aaattttctt taccggatcg
    11701 aatcttataa atggtgactc cggatagatt tatgatagat atttttgaaa aactgataat
    11761 aaaatctgtt caggattgcg gtttattatg aaatccatta ttcttgccgg gggtgtaggg
    11821 acaaggctgt ggccactctc ccgacagtat tatcccaagc agttcattcc attggacggt
    11881 cagtcgcttt ttcaaaagac ctatgaacgg gcaacccggc tttcggatcc agaggaaata
    11941 tttgtggtga ccaatgagat ccatcagtac ctcgttcgta accagattga gg
//
LOCUS       DASRLV010000125         6017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_610751,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000125 DASRLV010000000
VERSION     DASRLV010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610751"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VFC43_04975"
     CDS             <1..594
                     /locus_tag="VFC43_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30572.1"
                     /translation="IPLPTMTSPAILLPKSIKDSPLLFTISAPEGFAGTTIRATTYEY
                     NILYKTTIFNPANGDTNTTINNNSGTYIPLSDSLTVFSYSSSLSVDQNIRNIIRGSGA
                     VFTESSVTYNGITYIRFDAVSDPYSRMPAETVIFVANKGSSNERGFLPVIIYTMTPSG
                     TLSQATYENMVKSFRYYTARTISTASGVETDRPSFYQ"
     gene            complement(820..1800)
                     /locus_tag="VFC43_04980"
     CDS             complement(820..1800)
                     /locus_tag="VFC43_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30573.1"
                     /translation="MGRSQERLFPVRNFPLLAGFLLLIAFVIVLWISPVAAADMASES
                     ARPLITSPAVVHVGVYVVDFKRFSVEEGTVETTFYLTIRSDTNITVSDLEFMNGRVTS
                     IDVIRDTPFEKNYRIYAVMAVDPDLRHFPFDTHNLPLLIEHKTLDGDHLRFIIDEKET
                     GLDEEASIPGWTFTGNRFTVTNRSYAPGEVPYSRAVFSYGITRDSTSTILKFFLPIGL
                     IIFVSLASLLMKVSSRLGLNASMFLAAVLIHWRVVDAIPLVAYATFLDIFMIITYATL
                     VMVLVSGILILRYNEAKDSSRVEQISRWSLRIIPAISLAMYGLLFLSLIS"
     gene            2009..2887
                     /locus_tag="VFC43_04985"
     CDS             2009..2887
                     /locus_tag="VFC43_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZK30574.1"
                     /translation="MDEPVPKRSLKIAIALTSVIFLAEVAGGLWSGSLSLLGDAAHML
                     QDVVALLLSLGAIIIAEKMPTSSRTFGYHRVEIGAAVANGFFLIVVSVVIIREALSRF
                     SHPGMVNSTVMLAIALIGLVANLLSMYVLHGSHDLNVKSAFLHVLGDTLSSVAVIGAA
                     AWIAITGQTIVDPLLGIVIAVIILFSSFSLLRDAFTILLQFAPRDVDTEDVIREMESV
                     AGVEGVHNVHLWSLCSNINVLDAHVYSCDTDARNLETIKTEIKHRMEKFKIRHSTLEF
                     ECRECADCRTHRELRD"
     gene            3019..3384
                     /locus_tag="VFC43_04990"
     CDS             3019..3384
                     /locus_tag="VFC43_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30575.1"
                     /translation="MSSITAGTSNYESFNKILHRLGISMSVRVKFMSSGAFHSGVSKY
                     LDLGQISKLQISRQRNSCELQATTPENPQPSKPYIIARRDQEYELKDILNDLQQLRVE
                     KRDATYEITDTETRFVLTV"
     gene            complement(3398..3583)
                     /locus_tag="VFC43_04995"
     CDS             complement(3398..3583)
                     /locus_tag="VFC43_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30576.1"
                     /translation="MDERLIAGYIMAIAGFVMILVNALSYIFHGDLRSPAFTILGLVL
                     IVIGARTAKRSSWIQIQ"
     gene            3842..4690
                     /locus_tag="VFC43_05000"
     CDS             3842..4690
                     /locus_tag="VFC43_05000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZK30577.1"
                     /translation="MFSGIGDIAATSFLTLYCHALETLSKNPLLADPKSVEITTVLNK
                     TLAGSTDPLHRILVSGMIDKRLIVHIALRAKKYDEYTRNFLQKSPDGIVVNIGCGLDS
                     RFLRIDNGRVIFYDLDLPEIIEIKKGFFHESDRYHLVASSVLDFDWMTVVRQQKGPFL
                     FMAEGVFMYLDGKDVRSLVLMLQEKFPGSELVCEVANSLWLRPLFRKILDYKMQKQAH
                     LGKNAMFRSGIHNGQEMEQWHSGIQLLDEWSYFDSNEEKLGWLRVLRHIEFIRKTQWT
                     VHYRLG"
     gene            complement(4883..5689)
                     /locus_tag="VFC43_05005"
     CDS             complement(4883..5689)
                     /locus_tag="VFC43_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286145.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HZK30578.1"
                     /translation="MKTIIYYFTGTGNSLAVAKKIAAVLGDCELVPIALLQTTKGDII
                     PAADRVGIINPVYFAGLPVMVAEFAGRLDLPEAQYVFSIVTFGGSGAAPALQQLDSIL
                     KKRRDHGLDAGFMVKMPGNYILMYESPTGEKRERILAAAEAQVSDSAAAIKRNEILPL
                     PSAFLSGILHSIAYPHFASHVHEDDRKFSVNEKCTSCGTCAAICPAGNIDMVDGKPVW
                     KHQCELCCGCIHLCPEEAIQAGSRTAARQRYHNPSVSIEELKGNARDKRP"
BASE COUNT         1495 a         1515 c         1462 g         1545 t
ORIGIN      
        1 attcccctgc caactatgac ctcacccgca attcttcttc caaaaagcat taaggactcg
       61 ccgcttctct tcaccatctc cgcccctgaa gggtttgcgg gcacaaccat ccgggcaaca
      121 acatatgagt acaacatcct ctataagacg accatcttca acccggcaaa tggtgacacc
      181 aacacgacca taaacaacaa cagtggcacg tacatccctc tttccgattc gctgacggtc
      241 ttctcgtact cctcgtccct cagtgttgac cagaatatcc ggaatattat ccggggttcc
      301 ggtgcggtat tcaccgaatc ttccgtaaca tataacggta ttacgtacat acggttcgat
      361 gctgtcagcg atccctattc ccgaatgccg gccgagacgg ttatctttgt tgccaacaaa
      421 ggttcgtcaa acgagagagg ttttctcccc gtcattattt acaccatgac tccgagtggc
      481 acgctcagcc aggcgaccta tgagaacatg gtaaagtcct tcaggtatta caccgcgagg
      541 accataagca cggccagcgg tgtggagacg gaccggccct ccttctacca gtaattctcc
      601 cttttttaaa ccggatgacc ggtttatcct tcaagcgacg gatactccgg tctgcactgc
      661 cattctgacc tccgttgagc acagggtatc ccgccgtttt atcgtgcatg gtttgtatag
      721 cccagattcc tcgtgcagtg catccccttt ttaaaaatga aatcaatctt ggaatcgtga
      781 cgggcttttt catttctgaa aaggagggtg ggcttcttct caacttatga gtgagaggaa
      841 gaggagaccg tacatcgcca gcgagattgc aggaatgatc cgaagagacc agcggctgat
      901 ctgttcaacc cgtgaagaat ccttcgcctc attatatctc aggatgagga tacctgatac
      961 cagcaccata accagggtcg cataggtgat gatcataaag atatccagaa atgttgcgta
     1021 tgcaacgagc gggatcgcat cgacgacccg ccagtggatg agcaccgcgg caaggaacat
     1081 cgatgcgttc agtccgagac gggaagagac tttcattaac agagatgcaa gggagacgaa
     1141 gatgatcagg ccgatgggca ggaagaattt gaggattgtt gatgtgctat cccttgtgat
     1201 gccatagctg aagaccgccc gggagtacgg gacttctccc ggtgcatagg accggttcgt
     1261 gacagtaaag cggttgcccg tgaacgtcca gcccggtatg gatgcctctt catcgagtcc
     1321 ggtctccttt tcatcgatta taaagcgcag atggtccccg tcgagggtct tgtgttcgat
     1381 gagcaaagga agattatgag tatcgaaagg gaagtgccgg agatccgggt ctactgccat
     1441 caccgcatag atccggtaat tcttctcaaa gggtgtatca cggatgacat ctatagaagt
     1501 tacccgtccg ttcatgaatt cgagatcgct gaccgtgata ttggtatctg acctgatagt
     1561 gagatagaat gtggtctcga ctgtcccttc ctcgacactg aaccgcttga aatcaaccac
     1621 atataccccg acatgcacaa cggcgggtga agttatgaga ggccgggcag attcgcttgc
     1681 catatcagct gcggcaaccg gtgatatcca gagtacgatg acgaaagcga taagcagcag
     1741 gaaaccggcg aggaggggga agttacggac aggaaacaac ctctcctgcg accgtcccac
     1801 tcctccatta ttattcagca atcgggaaat ggtatcgtct tctccttaaa aaatctggtg
     1861 acgtcccggt atattaattg gaatggatcg gtaaatcagt gttgatcttt tactgacccc
     1921 tggtctccct atggaatgtt ctggttatat acccggagag aaatactatc tatcgatgca
     1981 taaccatacg gcagaatccg gaggaagtat ggatgaaccc gtcccgaaac ggtcactgaa
     2041 gatcgccatt gctctcacct ctgtcatatt tcttgcagag gttgccggcg gtttatggtc
     2101 cggttccctt tccctccttg gggatgccgc ccatatgctc caggacgttg ttgccctcct
     2161 cctctcgctt ggggcaatca tcattgcaga aaagatgccg acctcgtcac ggacgttcgg
     2221 ctaccaccgc gtggagattg gagctgcggt tgcaaacggg ttcttcctga tagtcgtcag
     2281 cgttgttatc atccgcgagg cactgtcccg gttttcccat cccggtatgg tgaacagtac
     2341 cgtcatgctc gcaattgcct taatcgggct tgttgcaaac cttttgtcaa tgtacgtcct
     2401 gcatggcagc catgacctga acgtgaaaag tgcttttttg cacgtactgg gcgataccct
     2461 gtcatcggtt gcggttattg gtgcagcggc atggattgcg atcacgggcc agacaattgt
     2521 agatcctctc ctcggtattg tgatcgcggt cattatcctg ttctcatcat tctcgctcct
     2581 gagggatgcg tttaccatcc tcctccagtt tgcgccccgc gatgtggata cggaggatgt
     2641 catccgggag atggaatcgg ttgcaggtgt tgaaggagtg cataatgtcc atctctggag
     2701 cctctgctcg aatatcaatg tgctggacgc ccacgtctat tcgtgtgata cggatgcccg
     2761 gaatctcgaa acgatcaaaa cagagatcaa acaccggatg gagaaattca aaatccgtca
     2821 ctctaccctg gaattcgaat gccgcgaatg tgctgattgc cggacacacc gcgagctgcg
     2881 ggactgactc acgtatcacg ttttgcggta ttttcccggc agcggtttta ttttcagaca
     2941 tccctcaggt tcccgggact ggtcctttct cgtttcagac aaacccctcg gcatatacat
     3001 ctatgagccc gcgaaccatt gtcatccatc acagcaggaa ccagtaacta tgaaagtttt
     3061 aacaaaattc tccacaggct cggcatttcc atgagcgtgc gggtaaaatt catgtcatcg
     3121 ggcgccttcc attccggggt atccaaatac cttgatcttg gtcagatctc aaaactccag
     3181 atctcccgcc agcggaactc ctgcgaactc caggcaacaa caccggaaaa tccccagccg
     3241 agcaaaccct acatcatcgc acggcgggac caggagtacg aattaaaaga tatcctgaac
     3301 gatctccagc agctgcgggt agaaaaaagg gatgccacct acgagattac cgatacggaa
     3361 actcgtttcg tattaaccgt ataagcaaaa gtgcctttca ttgtatctgg atccagctgg
     3421 atctcttagc agtccgggcg ccaatgacaa ttaaaacgag gcccaagatg gtgaatgcag
     3481 gagatctgag gtccccgtgg aaaatataac tcagggcatt taccagaatc atgacaaatc
     3541 ctgcaatcgc cataatatat cccgctatca gccgttcatc catgatttta tatccggacg
     3601 atccctgatt gaattttttt atacatcatt ctctgttatg ccctcttttc cttgtgtgtg
     3661 tcaaaggcaa atctcaaatc agatatactg ggacaaaggg atttttttta gctgcgagga
     3721 ttaattactg actataccga agccggattt tgtcagctca tttttccaaa ccttttaaaa
     3781 agattaaaca ttaccttatc agatagttgt ctcgtcccgg gatcatatgt cagggtggaa
     3841 aatgttttct ggaatcggtg atattgcggc tacgtcattt ctcactctct attgtcatgc
     3901 ccttgaaacc ctttcaaaaa accccctgtt agccgatccg aaatctgtag agatcacaac
     3961 cgttctgaat aaaacgctcg ccggttcaac agacccgctg cacagaatcc ttgtatcagg
     4021 tatgatcgac aagaggctca ttgtccatat tgccctccgc gcaaaaaaat atgatgaata
     4081 taccaggaac ttcttacaga agagtccgga cggcattgta gtcaatatcg ggtgcgggct
     4141 cgattcccgg ttcctgcgaa tcgataacgg gcgtgttatc ttttatgatc ttgatctgcc
     4201 ggaaataatt gagattaaaa aaggcttttt tcatgagtct gacaggtacc accttgttgc
     4261 ctcgtcggta ctggattttg actggatgac tgttgtccgg cagcagaaag gccccttcct
     4321 gtttatggct gaaggtgtat tcatgtatct tgatgggaag gatgtcagat cacttgttct
     4381 gatgcttcag gaaaagttcc cgggctctga gctggtctgt gaagtggcaa actccctctg
     4441 gctgaggccc ctgttcagaa aaatcctgga ttacaagatg cagaaacagg cgcatctcgg
     4501 aaaaaatgcc atgttccggt ccggcatcca taatggccag gaaatggaac aatggcatag
     4561 cgggatacag ttgctcgatg aatggagtta ctttgattcc aatgaggaga aactggggtg
     4621 gcttcgagta ctgaggcaca ttgaattcat acggaagacc caatggactg tccattaccg
     4681 gttggggtga tgttcaaaaa agggccgtgg gatccttacg tgaggatcct ctggactgat
     4741 tttttaaaaa gtgctccgcc agagctgacg cagaaagtag cggttattgt gctgaaccgg
     4801 cacgccccgg ccttgtgcaa aagggaggtt atgatgagtt tttggcagga cctgtcctca
     4861 gtttttatcc catggattct ttttatggtc tcttgtccct ggcatttccc ttaagttcct
     4921 ctatgctgac cgaaggatta tggtaacgct gccgggcagc agtgcgggaa cctgcctgga
     4981 tggcttcttc cgggcagagg tgaatacacc cacagcagag ttcgcattgg tgtttccaga
     5041 cgggttttcc gtccaccata tcgatatttc cggcagggca aatggctgca caggtgccgc
     5101 aggacgtgca tttctcattc accgagaatt tccggtcatc ctcgtggacg tgggatgcaa
     5161 aatgcgggta tgctattgaa tgcagtatac cggaaaggaa cgcagagggc agtggaagta
     5221 tttcgttccg tttgattgca gccgcactat ccgaaacctg tgcctcggcc gcggcaagaa
     5281 tcctctcccg cttctcccct gttggggact cgtacatcag gatataatta ccgggcattt
     5341 taaccatgaa ccccgcatcc agcccatggt cccgccgctt ctttaaaatg ctgtcaagct
     5401 gctgtaatgc cggcgccgcc ccggaacctc cgaaagtgac gatggagaaa acatattgtg
     5461 cttccgggag atcgaggcgc ccggcaaatt cagctaccat gacgggcagg ccggcaaagt
     5521 ataccggatt tattatgcca acccggtcag ctgcagggat tatgtccccc ttggtagtct
     5581 gcagcaaggc aatgggcaca agctcacagt ctcccaggac tgcggcgatc ttttttgcaa
     5641 cggcaagcga gttgccggtg ccggtaaaat aatagatgat ggtcttcatg gtaatcttaa
     5701 aaatccatta tcttctctac ttcatcagaa ttacgtaggg aataaaatag atttcctccc
     5761 cgcgcttccg tagatacaat cctaccggta tttataccat ttcagttcat gtgtcctggg
     5821 atttgcggga ctcccgatgg aacacatacc atacacttca cctttttgca tactggcatg
     5881 acaagaaaaa cattgtcctg ttggcatata gccgagcacg aagggggtgt tatccctatg
     5941 caaaaggata aaatttatca tgtttccatt agatgctgtg gatatgaaaa ggatcatact
     6001 catcgcatta tgcttac
//
LOCUS       DASRLV010000126         2833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_534993,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000126 DASRLV010000000
VERSION     DASRLV010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534993"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VFC43_05010"
     CDS             complement(<1..358)
                     /locus_tag="VFC43_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZK30579.1"
                     /translation="MISVLFVDDNSDLFGQIRSFLEKAGDIRIDKAHSIKNAMEKIKG
                     RTYDVIVSYERIPEVNGIEFMSDMNGIEFLQYLKSLGNTTPVILLCRRPNNKIAVQEV
                     ANGSEITLPVSGDIRQS"
     gene            complement(493..993)
                     /locus_tag="VFC43_05015"
     CDS             complement(493..993)
                     /locus_tag="VFC43_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30580.1"
                     /translation="MTRNRLVLLSQFLIFLIPLNIYMWGDWLIVNAQWALFRYQQSAY
                     GNSLILGHKDLAYISLGQTTGIYNIAAALFWTVGAAILFIGLCITIFAVLKDDSGFIK
                     KASYFTLCGGIFLSLSALSRFFGGFSIPIGIPIILYIGWRMYQDNKEEEEHEEESGDD
                     EVSVSD"
     gene            complement(1481..1822)
                     /locus_tag="VFC43_05020"
     CDS             complement(1481..1822)
                     /locus_tag="VFC43_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZK30581.1"
                     /translation="MVDPVDEALKLIQSKPEGILQSELWKELGVDSRKCSRIVKKLEE
                     SGLIDRSEFKKDGIKTYLLRAKQMPVNPSDLLAGNELIPCINCELECIVEECHPLMDW
                     MYQLAIVQHTD"
     gene            complement(1842..2078)
                     /locus_tag="VFC43_05025"
     CDS             complement(1842..2078)
                     /locus_tag="VFC43_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LSM domain-containing protein"
                     /protein_id="HZK30582.1"
                     /translation="MVNGIVLPIKKVFGLVDSKISVEIKDEGRKLQGRLVAVDEYLNI
                     HMDETIEFVDNQRGRSLGTVVIRGNNILTIAPVI"
     gene            complement(2206..>2833)
                     /locus_tag="VFC43_05030"
     CDS             complement(2206..>2833)
                     /locus_tag="VFC43_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructose 1,6-bisphosphatase"
                     /protein_id="HZK30583.1"
                     /translation="VIDPSMHKGFVFEVHDLIKKRRIMFNTPEESYSLLAYIGAPSRY
                     VIKHIFKKDGTIGASTSTQRLNMMAGRYVGKDDPVMIVRSQSGFPAVGEVLDPFCIPV
                     LVAGWMRGSHHGPIMPVGLCDANCTRFDGPPRVVCMGFQVSDGKLIGPVDMFDDVGFD
                     RVRARCNEVADLIRAQGPFEPHRLSLDEMEYTTLPAVEKELTKRWEPL"
BASE COUNT          800 a          690 c          608 g          735 t
ORIGIN      
        1 ggctctggcg gatatcccca ctcaccggga gggtgatctc gctgccgttt gcaacttcct
       61 ggaccgcaat tttattgttc gggcgccggc aaagcaggat taccggtgtg gtgtttccga
      121 gcgactttaa gtattgtaaa aattcgatgc cgttcatgtc agacatgaat tcgatcccat
      181 tcacttcagg tatccgctca taggaaacga tgacatcgta cgtccggcct ttgattttct
      241 ccattgcatt ttttatggaa tgtgctttat cgatccggat gtctccggct ttttccaaaa
      301 aagagcggat ctggccgaaa agatcggaat tgtcatcgac aaataaaaca gaaatcatac
      361 atctcccggg ggaaagtgcg gattattcac ttatatgagg tggatagttt tccgataaat
      421 aagttgtccg agcatgccgg ccatccagaa aaagaacacc ccacaagaaa aaacgaaaag
      481 aacccaaaag cattagtccg agaccgaaac ttcgtcgtct cctgattcct cttcatgctc
      541 ctcttcttct ttattgtcct gatacatccg ccagccgata tagagaataa tagggatccc
      601 tataggaatg gaaaaaccgc caaaaaaacg actcagggca gataaactca aaaagattcc
      661 gccacaaagg gtaaaatacg aggctttttt gataaaacca gaatcatcct taagaacagc
      721 aaaaatggtg atacaaagtc cgatgaaaag aatcgctgct ccaactgtcc agaataaggc
      781 agccgcaatg ttataaatgc cagttgtctg gccaagggaa atataggcaa gatctttgtg
      841 cccgaggatt aggctgttac catatgcaga ttgctgataa cgaaacagtg cccattgcgc
      901 attgacaata agccagtccc cccacatata gatgttcagg ggaatcaaaa aaatgaggaa
      961 ctgtgataag agcactaacc tgttcctggt catggtaaac aggatagata tttttcgcct
     1021 tccgctatga gcctatcgaa atacatgaat gactacaaaa aaatttaatc tgtagaaaca
     1081 tgcataaacc catatgttcc cacccgaacc ttgaaaatcg ggtttctcta cgataaaacc
     1141 ccgcctctgg gcctctcttt aggcatggcg ggcaaggaac ttttttttag tcactagtcc
     1201 gccgcggcgg agtcccttcg ggagcgccgg aattcatcgt aattgaaaaa aagacatgat
     1261 aatccctaat tttacaggaa tttttcgatt ttcaggtgaa atctttttta aaaattctga
     1321 cgctcctttg ggcttagccc gcgtcaccag gacatcccca ttgtgataac aatgtattac
     1381 ctgctatcaa aatcgggtta ttgcactggg ccggccccaa tgataattgg tagcgcaaga
     1441 atgtttctcg gaaaaaagat tttcttcaga acaaaaaaat ttaatctgta tgctggacaa
     1501 tggccagctg gtacatccag tccatgagcg ggtggcattc ctcgacaatg cattcaagct
     1561 cacagtttat acaggggata agttcattcc cggcgagcag atctgacgga tttaccggca
     1621 tctgtttggc acgcaggagg taggtcttaa tcccgtcttt tttaaactcg gatcgatcaa
     1681 taagcccgct ttcttcaagt ttcttgacaa tcctcgaaca tttacgacta tcgacaccca
     1741 gttccttcca gagttcgctc tggaggatac cttcgggttt cgactgaatc agtttaagtg
     1801 cttcatcaac aggatctacc acagtaccca cctttttatc cttagatcac gggtgcaatg
     1861 gtaaggatat tattgcccct gatcactacg gtgcccaggc tccttccccg ctggttatcg
     1921 acgaactcaa tcgtctcgtc catgtgaata ttcaggtact cgtccactgc aacaaggcgt
     1981 ccctgcagtt tacgcccctc atccttgatc tcgactgaaa tttttgaatc cacaagtccg
     2041 aacacttttt taatcggcaa aacaattccg ttaaccatct gccctaattt gggatgtaag
     2101 ataattaaag cttgctcttc aggagaccag tttttctgca atgaataaaa cagatggcga
     2161 aataaaaaca cactattctt ccggataata aaaaaatatt tgattctaca gaggttccca
     2221 ccgttttgta agttcctttt caacagccgg aagggttgta tactccattt catccagtga
     2281 cagccggtgg ggttcgaagg ggccctgagc acgtatgaga tcggctactt cattgcaacg
     2341 cgccctgacc cgatcaaatc cgacatcgtc aaacatatcc accgggccaa taagtttccc
     2401 gtcactgacc tgaaaaccca tgcagacaac gcggggaggg ccatcaaacc gtgtgcagtt
     2461 ggcatcgcat aatccgacgg gcataatcgg gccatggtgg gacccccgca tccagccggc
     2521 aacaagaacc ggtatacaga acgggtcaag aacttcgcca actgcgggaa aaccactctg
     2581 ggaacggacg atcatgaccg ggtcatcttt tccgacatat ctccctgcca tcatgttcaa
     2641 gcgctgggtg ctcgtggatg cgccgatcgt accatctttt ttgaagatgt gcttgatgac
     2701 ataacgggat ggtgccccga tatatgccaa aagactataa ctttcctcgg gggtgttaaa
     2761 catgatcctg cgctttttta tcaggtcatg cacttcaaaa acaaaacctt tgtgcatcga
     2821 ggggtcgatg aca
//
LOCUS       DASRLV010000127         3280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_450347,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000127 DASRLV010000000
VERSION     DASRLV010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450347"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(316..1059)
                     /locus_tag="VFC43_05035"
     CDS             complement(316..1059)
                     /locus_tag="VFC43_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30584.1"
                     /translation="MKFMTLLALVAAVGFILATGCVAQPKKDPGNATVSPTNTFTPFA
                     NATTVPVINTTNTTNATSTNATPALSGPLRISISGYYAGEPLPVFIDNQSVGNVTREK
                     PLDLNVKEGNHTVSVCVGLTCITDYADIVFAKRSFIDFGDRLRNEVEFPVPTVRLINY
                     YKNGNGVGVNVEFINPSQKDLLMSLEVSCGYTYIDSRSSLRMGDSVRTKASEYVEAGR
                     RVTRTVDLYFAYGSAYTFDEPILSEITYQ"
     gene            1505..3172
                     /locus_tag="VFC43_05040"
     CDS             1505..3172
                     /locus_tag="VFC43_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZK30585.1"
                     /translation="MSQPDPPLQEQTGQTPDSNHLPGNGSFRKTSLSRLAVVIPTHNN
                     ELTIGSLVLLAHPYAGHVIVVDDASLDRTVDIAQNAGADVIPAGYTGGRVNAILTGCK
                     HALGFDCSAVVFLDITSGHLIRDIPRLAAPVLAGEADLVIGSRYLMGRKGIPPYLFDK
                     PGNNHTLPDTCHVFYNSDPGSPFRALSVRAVCLLDLLPDSDQFEPAMVTLFARKGLAL
                     QEIPVKPRVELSSAVGDDTPRYRYKLIGVVVPAYNEELLIGDTLAGIPEFVARIYVVN
                     DSSTDRTQQVLDYYAKHDTSIVPIRHEVNRGVGAAIVTGYTQALEEGMDIIGVMAGDN
                     QMDPAFLTELLDPIIDRKCDYTMGNRIANPLYRAGMSTWRFIGNSILTMLTKIASGYW
                     QMMDPQNGYTAISSRALNQISLAKVYPRYGYCNDILVKLNVESFRIINVPHPSRYGLA
                     KSGIKYSTYIVNVSFLLLRDFLWRLKKKYIVLNFHPLVFFYVGGTLLTLLGLFGGLYA
                     LHYKFIQHQPIFIPGIASLIAFSLGLQTVFFAMFFDMQQEKSTNVWY"
BASE COUNT          829 a          850 c          743 g          858 t
ORIGIN      
        1 tctcaatact acgattttca taggaaatgc tcaatactaa gctaaacgct cacggggttc
       61 caccccaacc gctatggctc ccatgattgc gataatcccg gacaggtaat atgtcatcat
      121 cgctttaagc gcaggtggag gatggggaaa caggtactgt tcactccttc atcgttccag
      181 ttctttgttt tccatcatct ggtaaaagga attctccaga accatccgga ggaaaaaagg
      241 aatccgcccg aggtccgggc atcagtgata acctgtctct gaatccggac tgcctaaaaa
      301 aaacagatca aaaaattatt ggtatgttat ttctgagaga atcggctcat caaatgtata
      361 ggcactgcca taggcaaaat agagatccac cgttcgtgtt acacgacgac cggcttccac
      421 atattccgat gccttggtcc gtacggaatc tcccatccga agactgcttc tgctgtcgat
      481 ataggtatac ccgcaactga cctccaggga catcaggaga tctttttgag aggggtttat
      541 gaactccaca ttcacaccaa ctccattacc attcttataa taattgatca atcgcacggt
      601 aggcacgggg aattcaacct cgtttcgtaa ccggtcccca aaatcgataa aagacctttt
      661 tgcaaaaaca atatctgcat aatctgttat acaggtcagg ccaacacaga ccgatacggt
      721 atggttcccc tcttttacgt tcaggtccag gggtttttcc cgggtcacat tacccacact
      781 ctggttgtct atgaaaaccg gcagaggttc tccggcataa taaccgctga tggatatacg
      841 caacgggccc gataatgccg gtgttgcatt agtgctggta gcatttgtgg tattggtcgt
      901 attgataaca ggaacggtcg tagcatttgc aaaaggggta aacgtatttg tgggagatac
      961 tgttgcattc ccgggatcct tttttggctg ggcaacacat cctgtagcaa ggataaaacc
     1021 gaccgcagca acaagagcga gcagcgtcat aaatttcatg cgaatttctc cgttttttga
     1081 agatggggtt gtgtcatttt tcaccgtgta acaatatata catgtatgta ttcctgtacg
     1141 tgtttttatt ctttcggcat ccggcccgtg atttttatcg ttttttactc aaacaacccg
     1201 ttttacacta tacacgccgt gaacagcggt tactgtgtcc tgagaatcct ttccatcacg
     1261 catagtttca ggcggatggc cctcctctgt agaagcacat cacaggattc ggatcctgtt
     1321 ttcattattc gccccgccag cataagaaaa aatgcacgac accagtgcca tgcaaataat
     1381 tatccggaaa aaaatgtttg aaaattaccc acaaacaaag tattacattg aatacaatat
     1441 gagtaaaata taatagaact aatccattga gtgttgattt ttctctcatc tcatgtccga
     1501 taccatgtca caaccggatc cccctcttca ggagcagacg gggcagaccc cagactctaa
     1561 tcacctgccg ggtaatggga gttttcggaa aacgtccctg tcccgccttg ccgttgtgat
     1621 cccgacccat aacaatgaac ttaccattgg cagcctggtc cttctggccc acccgtacgc
     1681 tggtcatgtc attgtagtgg atgacgcctc gcttgaccgg actgtcgata ttgcacagaa
     1741 tgcgggagct gatgttattc ccgccggtta tacgggtggc cgggtgaatg ctatccttac
     1801 cggctgcaag catgccctcg gctttgactg ttcagcagtt gtgttccttg atatcacgag
     1861 cggccatctt atccgtgaca tcccccggct tgcagcaccc gtgcttgccg gtgaagcgga
     1921 tcttgtgatt ggatcccggt accttatggg aaggaaagga atacctccct acctgtttga
     1981 taaacccggc aacaaccata ccctgccgga cacctgccat gtgttttaca actccgaccc
     2041 gggatccccg ttccgcgccc tgagcgtcag ggctgtctgc ctgctcgacc tcctgcctga
     2101 ctccgatcag ttcgaacctg ccatggtgac gctgtttgcc aggaaaggac tcgcattaca
     2161 ggagatcccg gtcaagcccc gggttgaatt atcatcagcc gttggcgatg acaccccgcg
     2221 ctaccggtac aagcttatcg gtgtagtcgt acctgcatac aacgaggaac tgctgattgg
     2281 tgatacgctt gcaggtatac cggaatttgt cgccaggata tatgtagtaa atgacagttc
     2341 cacggacagg acccagcagg tcctggatta ctatgcaaaa catgacacat ccattgtccc
     2401 tatccggcat gaagtgaacc gtggggtcgg tgccgcgatt gtcaccgggt atacacaggc
     2461 acttgaagag ggcatggata tcattggtgt catggccggt gacaaccaga tggatccggc
     2521 gtttcttacc gagctccttg accccatcat tgacaggaaa tgtgattaca ccatggggaa
     2581 ccgtatcgcc aatcccctgt atcgtgcagg gatgagtaca tggcgcttta ttggcaacag
     2641 catccttacg atgctgacca aaattgcctc aggttactgg caaatgatgg acccccagaa
     2701 cgggtatacc gcaatctcca gccgggcact caaccagatc agcctggcaa aggtgtaccc
     2761 gagatatggt tactgtaatg atatcctggt gaagctcaat gtcgaaagct tccggattat
     2821 caacgtccct cacccgtccc gttacggcct tgcgaagtcc ggcataaaat acagcaccta
     2881 tatcgtgaac gtatccttcc tcctgctgag ggactttctc tggcgtctca agaaaaaata
     2941 catcgtcctc aacttccacc ctctggtatt tttctatgta ggcggaaccc tcctcaccct
     3001 cctgggactt ttcgggggac tctatgccct gcattacaag ttcatccagc accagccaat
     3061 ttttatcccc ggtatcgcct ccctgattgc attctctctc gggctccaga ccgtattttt
     3121 tgctatgttc tttgacatgc agcaggagaa gtccacgaac gtgtggtatt gagtaccagg
     3181 agggagtgag ggtacatact atagatatac gtatttttta ctgtaaattt tataaaaaat
     3241 aagagaccaa aaaacggtga tccagcacat cattgatgca
//
LOCUS       DASRLV010000128         3139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1177644,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000128 DASRLV010000000
VERSION     DASRLV010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177644"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VFC43_05045"
     CDS             complement(<1..415)
                     /locus_tag="VFC43_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HZK30586.1"
                     /translation="MFNNILVALDGSEVSQRALVRAADEARLWKAKLHAIYVVETGLF
                     SSLPLDNTVEIMYRVLEKEGETVLEKAKKYCSEKGITVVTHMKQGHAGSEIITLAEHE
                     KSDLIIVGSHGKSNTDRLLIGSVSTFVVTHSKATTM"
     gene            complement(454..1494)
                     /locus_tag="VFC43_05050"
     CDS             complement(454..1494)
                     /locus_tag="VFC43_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZK30587.1"
                     /translation="MKNIPLMVSGQAFVGVDLALRPVDIVIENGIITAIEENPKAPMV
                     WICPAFFNAHTHLGDTIAMDCGVKGDLVSLVTPPEGLKHRLLAAASHRDLVSGMHASI
                     RGMIAGGTAGCADFREGGVAGVSALREATGGLSFQPFIFGREGGEGVADGLGISSTRD
                     STGIETRVADAHRLGKKIAVHAGERDAEDIDDALALDPDLIIHCTHATKKQIRECADR
                     QIPIAVCPRSNWSLGVTSSAHHPPLKLMQDLGCRIVLGTDNVMFVSPDMFSELSFTSM
                     VYRIDPQILLRAAVQGSELVGKSYFIREGVRANIFTIDVTQSALLFSRDPVTSLIKRG
                     SGRQISNNVFNL"
     gene            1540..1710
                     /locus_tag="VFC43_05055"
     CDS             1540..1710
                     /locus_tag="VFC43_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit Sec61beta"
                     /protein_id="HZK30588.1"
                     /translation="MAKKQGGRLLSSAGLVNYYESEDRRAVHISPITVIAVASAIGII
                     IMILNIVYGLNA"
     gene            complement(1746..2501)
                     /locus_tag="VFC43_05060"
     CDS             complement(1746..2501)
                     /locus_tag="VFC43_05060"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HZK30589.1"
                     /translation="MEQIQVMGVRGLPLIHPGDNIAAMVCDRMPVEDGDILCVASTIY
                     SKAKGYTKTLASIIPSERAQRLAILNGEDPRFVQAVLDASKDIIMEHPFILSELSFGH
                     IGVRAGVDQSNIEDGKVIFLPPDPMGAAQELREDIRTLVNKNIGVIITDTCGRSFRRG
                     QTGNAIGWSGFYAIRDFRGDTDLFGHVLKITEEAVVDEIAGFANFVMGESNNGVPAVI
                     FRNCPKWNGHDNLYFRNDEDITKRVLKKNGAMR"
     gene            2693..2974
                     /locus_tag="VFC43_05065"
     CDS             2693..2974
                     /locus_tag="VFC43_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1922 domain-containing protein"
                     /protein_id="HZK30590.1"
                     /translation="MYLIIRCPSCRTFTYVDRFQKWKLCPICGHAQEVVKAPAYLEVE
                     HYLEAEHIVKQMEKHLHTTKKPDFSPEETAELRHHYTEALRKKAKSRYS"
BASE COUNT          827 a          795 c          679 g          838 t
ORIGIN      
        1 ccattgtcgt tgctttgctg tgggtcacta cgaatgtgct gacgctgccg atgagaagac
       61 gatcagtatt actcttgcca tgagaaccga caataataag gtcagatttt tcatgctcag
      121 caagagtaat aatctcactc ccggcatgcc cctgtttcat atgggtgacg accgtgatac
      181 ccttctcgga acagtacttt ttagctttct ccagtactgt ttccccctct ttttcaagaa
      241 cacggtacat tatttccacg gtattatcaa ggggaaggga agagaacagg cctgtctcta
      301 ccacatatat ggcgtggagt tttgctttcc ataatctcgc ttcatcagca gcccggacaa
      361 gcgcgcgctg gcttacctcc gatccatcta gtgcgacaag tatattatta aacacagata
      421 tcccccaaaa caaacatgcc tcttttattg gatttacaaa ttaaaaacgt tattgcttat
      481 ttggcgacca gaaccccttt ttatcaaaga agtaaccgga tcgcggctga ataagagagc
      541 tgattgtgtg acatctatag tgaagatatt tgcccgaact ccttcgcgga taaaatacga
      601 tttcccaacc agctcagaac cctgaacagc tgcacggagc aggatttggg gatcaattct
      661 gtataccatt gatgtaaatg acaattcaga aaacatatcc ggggacacga acatcacatt
      721 gtcggtacca agcactattc tgcaccccag atcctgcata agtttcagag gcgggtgatg
      781 tgctgaagat gtaaccccga gcgaccagtt ggacctggga cagactgcaa tggggatctg
      841 cctgtctgca cattcacgaa tctgtttttt tgttgcatgt gtgcagtgaa taataaggtc
      901 aggatcaaga gcaagtgcat cgtcaatgtc ttccgcatcc ctttcaccgg catgcacagc
      961 tatttttttc cccaaacggt gtgcatctgc aaccctcgtt tcaattcccg tactatcccg
     1021 ggtgctgctg atccccagcc catcagcaac tccttccccc ccctcacgac caaaaatgaa
     1081 aggctggaag gacaatcccc cggtagcttc tctgagtgct gatacacctg caactccccc
     1141 ttcacggaag tctgcacagc cagcggtacc cccggctatc atcccccgta tactcgcatg
     1201 cattcccgaa accagatccc ggtgtgatgc ggcagcaagc aggcggtgtt tcaggccttc
     1261 cggcggagtg acaagggaca cgagatctcc tttcaccccg cagtccatcg caatggtgtc
     1321 accaagatgc gtatgcgcat taaagaatgc aggacatatc cataccatgg gtgcctttgg
     1381 gttttcttct attgccgtaa tgataccgtt ctcaataaca atatcgaccg gccggagtgc
     1441 aagatccacc ccaacgaatg cttgtccgga aaccatgaga gggatgtttt tcatacgccg
     1501 taactctcct ttatatattg aaaaatgaaa tatatttgca tggcaaagaa acaaggtggc
     1561 agattacttt catcagctgg tctcgtcaac tattacgaaa gcgaggaccg gcgggctgtt
     1621 catatcagtc ccatcacggt gattgcagtc gcatcagcga ttggtatcat cattatgatc
     1681 ctgaatattg tctatggatt gaatgcttaa ctctcctttt ttttaacaat tttagttctc
     1741 tgccattatc tcatggcacc attctttttc agcacacgct ttgtgatatc ctcatcattt
     1801 ctgaaataga ggttgtcatg cccattccat tttgggcagt tgcggaaaat aactgcgggg
     1861 acaccgttgt tgctctctcc cataacaaag ttagcaaagc cggctatctc atcaacaaca
     1921 gcttcttcgg ttattttgag cacatgcccg aacaggtcgg tatctccacg gaagtcccgt
     1981 attgcataaa aaccgctcca acctattgca tttcctgtct gcccgcgacg gaatgatctg
     2041 ccacaggtgt cggtgatgat aaccccaata tttttattga ccagtgttct gatgtcctcc
     2101 cgaagttcct gtgcagcacc catcggatca ggtggcagaa agatcacctt gccgtcctca
     2161 atattgctct ggtcaacgcc ggctctgacc ccaatgtgcc caaaagagag ttcagagagg
     2221 atgaaggggt gttccatgat aatatcttta gatgcatcaa ggacggcctg gacgaaacgg
     2281 gggtcttcac cattcaggat ggcgagccgc tgagcccgct ctgatggtat gattgatgca
     2341 agagtttttg tgtagccttt agctttggag taaatggttg aagcgacaca gaggatatcg
     2401 ccatcctcga ccggcatccg gtcacatacc atcgcagcta tattgtcacc aggatgaatg
     2461 agggggagtc ctctcacacc catgacctga atctgttcca tagtaacatc tttatgagaa
     2521 gatctgataa taaaggagtt tcgttttgtt tcgcttcctc cgttcggaga ataagcgtac
     2581 acttcgtgcc tttcagcatc acatagtgat acttcctttt acctccccgt tcctggtatt
     2641 taacaatcag ccgatatatt tttatcagga taacacgcat cacctctttg tcatgtacct
     2701 cattatacgg tgcccgtcct gcaggacttt cacgtacgta gatcgcttcc agaaatggaa
     2761 gctctgtcca atctgcgggc atgcccagga ggtagtgaaa gcgccggctt accttgaggt
     2821 tgagcactac cttgaggcag aacatattgt aaaacagatg gaaaaacatc tccacactac
     2881 aaagaaaccg gatttttcac cagaagaaac tgcagaactg cgtcatcatt atactgaagc
     2941 actgagaaaa aaagccaaaa gccggtactc ataatcctat ctgactattt ttttatgaat
     3001 gatctgaatc ttgagttaaa tccacgggat ttatttcatg gatagtaccc ctcacatatg
     3061 acgcgccgac accagcaata cagacaaccg tgaaaatcag gcatgaaacg gttgtgctga
     3121 caaggagtga gggatatac
//
LOCUS       DASRLV010000129         7581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_777853,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000129 DASRLV010000000
VERSION     DASRLV010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 7581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7581
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777853"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..662)
                     /locus_tag="VFC43_05070"
     CDS             complement(99..662)
                     /locus_tag="VFC43_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30591.1"
                     /translation="MCLPEKGWKKTGTRRRAEYENTAGQLYTIEGIAAGLIMIMTAYL
                     VVSATSVYTAGDTHISDMQLEQLGTDALKVMDAPASRGENSLLQDIIEKTPVGTTNTT
                     FGLVFSDLTNSHTTSVKDHIQYTASYSYRNSTDVSSAFLYASRNLSAGEHAVRVTKWV
                     IVNKLLPGDSNPPKDRAVLVEVLMWRD"
     gene            complement(667..2223)
                     /locus_tag="VFC43_05075"
     CDS             complement(667..2223)
                     /locus_tag="VFC43_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30592.1"
                     /translation="MTDDYGNLSIDFLVGFTFFILSFIWVVTMIPGLLIGLQSYSIDY
                     DAVAYRTGVILVEDPGWPASPPWESYNDLQKFNVTRFGLANSRDTPNILSEDKVNRFF
                     CSTAFIYPDDYHQRAIFGDYPYHFQISLRETNESRKRVVGERLPQGYGSIRRVVTIKG
                     SSNATINQSYIQNHSYIKTDNVSIHVFSLLINNTKLLSDVRNPAYQIDAAREQITINI
                     TDMKLLTASTITLDNMKIYKLDAGTYSNIPLPVSNYPSADGNSTRISAFPATVIDNLT
                     IKLNPQYIDSMKAENSQIFLTLQFSVTPASTFLNNTQVVPDIAIMDGKMTFENPNTTP
                     FMYDYNPANVTQPKLRNAIVEVAVWSGETGLASATTTIFSDDFDAMSAPAGWTLIGPG
                     VRFTGTPHNGLASIRIRDNPGSITRTISTAGYSDITVSFEMGTQMSGGAMDADAEWSP
                     DGGTTWNLLKRINSGDPEDDNDLHPFTYPLPAGADNNPAFTLRFSLSGPATAGDKVYV
                     DNIEVGGIAD"
     gene            complement(2226..2798)
                     /locus_tag="VFC43_05080"
     CDS             complement(2226..2798)
                     /locus_tag="VFC43_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30593.1"
                     /translation="MRRNIPTIFSIMTLVALILICACLAQSPADQSSRPGGLTQITPA
                     SEGSRLSFETAKQNFLEYRSESTNDIETRKNVNYMLSRDMDDHGNARTWIFGVYGEKG
                     PVYLINDITGWTTIENVTLPSEKISLDTIVTPENLLKQNRAAILNNPSPGVTERWDLE
                     LQRGVYTLTITSGSSARSLIFNATTGALIS"
     gene            complement(2965..3837)
                     /gene="hisG"
                     /locus_tag="VFC43_05085"
     CDS             complement(2965..3837)
                     /gene="hisG"
                     /locus_tag="VFC43_05085"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HZK30594.1"
                     /translation="MITIALPKGSLEAQTLQLFKEADLEVRRTDRDYNPRIADSRIGK
                     IKILRPQEIPTYVEKGYFDVGISGLDWVNETGSDVVEVANLSYSKTGEGNVKIVVAVH
                     RDEPIDDVSRIRPQSRVTTEYPELTKKYFARLGIPVQLFHSFGASEAKVPDLMDVVVD
                     LTETGTTLRKNGLKIIGQIMESYTVIIANKASWADPDKRREIEEIKTLLFGVIEARNK
                     VLLTMNVPTHSMEKIVAALPAMKKPTVSRLHGIDYYSIQTVVPKNTVNQLIPKLKACG
                     AEDILEIPITKIVP"
     gene            complement(3889..5154)
                     /locus_tag="VFC43_05090"
     CDS             complement(3889..5154)
                     /locus_tag="VFC43_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HZK30595.1"
                     /translation="MVTIYRFSGMRPDRLAAPAIAAVPYDVVTADEARAIIAKNPETF
                     LRVSRADAELPDLSPYDERVYQRAYDNFIALEASGRMKKDPEPGMYLYRVLQDGETFL
                     GLCCCLDVDDYRKRNIRRHEQTRYDKEEDRTRHIEAVQAHNGPVVLLYRNRKEIFSCI
                     ESLADRSKTPDAEVTSDTGIIHQIYRIMDSKILDQLETIFSSVPSLYIADGHHRAKAA
                     LNVVDHRIAEGKPAEGEVKHFMGVMFAHDRVKIHGYSRLLTDLGDYSEEMFLDRLKDC
                     FSVKEYGPVNGNGYNIPPRISDPEKFHVIHLYISGKWFECTRPIDTRLTPLMSLDVAV
                     LQKHVLETMLGITDPRGDARLQYLGGARPVSDLEKLVNEGRYRIAFAMQPVKVDKVLA
                     IADADGVMPPKSTWFEPKLLSGLVVHTFS"
     gene            5317..5802
                     /gene="rimI"
                     /locus_tag="VFC43_05095"
     CDS             5317..5802
                     /gene="rimI"
                     /locus_tag="VFC43_05095"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HZK30596.1"
                     /translation="MIRPRDLSMYPLPLIRRAMPPDIAAIVEIENESFIDPWEPDVFL
                     EAFTFFPSTYFVAVCDGIVAGFIIGAPENTGENIYGHICNFGVSPVYRHRGIGSLLVK
                     RIEHQFMLELATAVQLEVRVSNTNAQKFYRRLGYRDVFRIDDYYFNGEDALVMMKWFR
                     F"
     gene            5949..7127
                     /locus_tag="VFC43_05100"
     CDS             5949..7127
                     /locus_tag="VFC43_05100"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HZK30597.1"
                     /translation="MQQPQPHLRYFCSVTFLTFLLCTGIAGAALTDHPVVNGISGPDH
                     LNDWFGPTGSYTFTATVTGGTPPYSYKWMNPPGMKILFEGKQYGTVEIPAPQLGLSGP
                     GNYGVWLTVTDSAGKDAVWLRQGGSGNSNQFGYFLTTDGKTWSKKTEPATFPPPVDAP
                     QVIQKTSDQCVDSGARFTGIQGDIQVSSECNGDYSKGIWHLAKREITLYGGDHIRTGE
                     DTTGYLGFQDMSTFILKAESEIVLLVPENKDSKLKLVAGNIWTNVKKMVKDGSMEIDM
                     DQAVLGTKGTTFVCSSDGKTSTMQIIEGTVEVTGKADKKKILVTGGQQVTATSKGLGT
                     PVAFDAPAAQADWDAVKAKALSGTPAPAPATTKKSGLESLLVLAAFGIVVAGIVFRRE
                     "
     gene            complement(7231..>7581)
                     /locus_tag="VFC43_05105"
     CDS             complement(7231..>7581)
                     /locus_tag="VFC43_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA reductase"
                     /protein_id="HZK30598.1"
                     /translation="FALAIVTMFIACGQDAAHAIDGSTCITTVDLTDAGAYVSVTLPS
                     LPVGTVGGGTGLETQQECLRILGVAGGGDPPGTNARKLAEIIGAGVLAGELSLLGALA
                     AQHLARAHQQLGRG"
BASE COUNT         1834 a         1880 c         1888 g         1979 t
ORIGIN      
        1 ctgcccgact accgtggact ggttgataat tatcgccagg aagaaaaaac tgaaactgat
       61 taaaaatccc atgaggataa tccactgtgc atcatcattc agtcgcgcca catcagcacc
      121 tccacaagta cagcccggtc cttcggagga ttgctatccc ccggaagtaa cttattcacg
      181 attacccatt tggtgaccct caccgcatgt tcccccgcac tcaggttgcg ggaggcatac
      241 aggaaagcac tgcttacatc cgttgaattc ctgtaggaat aactcgccgt gtactggata
      301 tgatccttca ctgaggtggt atggctgttc gtaagatcgg aaaataccag tccgaatgtt
      361 gtattggtgg tacccaccgg cgttttttca ataatatcct gcaacaggct gttttcccct
      421 ctcgaagcag gagcgtccat cactttgagg gcatcagtcc caagctgttc gagctgcata
      481 tcactgatat gggtatctcc ggcagtgtac acggatgttg cgctgacaac aaggtacgct
      541 gtcatgatca tgatcagccc ggctgcgatc ccctcaatcg tatagagttg tccggcagta
      601 ttttcatact cagctcgtct gcgggttcct gtttttttcc atcccttttc cggtaaacac
      661 atcgcgctaa tcggctatcc ccccgacctc tatattgtct acatatacct tatcaccagc
      721 agttgccggc ccactcaggc tgaacctcag agtaaaggcc ggattgttgt cagcacctgc
      781 cgggagcggg taggtgaatg ggtgcaggtc attatcatcc tcgggatcgc cggagtttat
      841 ccgtttcaga aggttccacg tggtcccccc gtctggtgac cattctgcat ccgcatccat
      901 ggcccccccg gacatttgcg ttcccatttc gaatgaaacc gtgatatcgg agtaccctgc
      961 agtggagatg gttcttgtta tacttcccgg gttatcccgt atccggatac ttgcaagccc
     1021 gttgtgagga gtaccggtga atcgtacccc aggacctata agagtccatc cggcgggagc
     1081 agacatggca tcaaagtcat cggagaaaat ggtggttgtc gccgatgcga gcccggtttc
     1141 ccctgaccat acggctactt caactattgc attcctgagt tttggttgcg tgacatttgc
     1201 cgggttatag tcatacataa acggtgtggt attggggttt tcaaacgtca ttttcccatc
     1261 cataatagcg atgtccggga ctacctgcgt attatttaaa aatgtagatg caggagtcac
     1321 gctgaactgc agcgtgagga aaatctgcga attttctgct ttcattgaat cgatatactg
     1381 agggttcagt ttgatcgtca gattgtctat caccgtcgcg gggaaggctg atatgcgtgt
     1441 cgaattgcca tcagcagagg gataatttga aaccggcagg gggatattgg aataggtacc
     1501 cgcatccagt ttgtatatct tcatattatc aagggttatg gtgctcgcag ttaataactt
     1561 catatccgtg atgttaatcg ttatctgttc tctcgctgca tcgatctggt aggcagggtt
     1621 cctgacatcg ctgagcagtt ttgtattgtt gatgaggagt gaaaatacat gaatactgac
     1681 gttatcggtc ttgatatacg aatggttctg gatatatgac tggttaatcg tcgcattgct
     1741 cgatccttta atcgttacca cgcgccgtat tgatccgtac ccctggggta aacgctctcc
     1801 taccacgcgc ttccgggatt catttgtttc acggagggag atttgaaaat gataggggta
     1861 gtctccgaag attgccctct ggtggtaatc atcaggatag ataaaggccg ttgaacagaa
     1921 gaaccggttc actttatcct ccgaaaggat attcggtgtg tccctcgaat tagccagacc
     1981 gaaccgggtc acgttaaatt tttgcaggtc attataagac tcccacggag gcgatgccgg
     2041 ccatcccgga tcctccacga gaataactcc ggtccggtat gctactgcat cgtaatcgat
     2101 actgtaggac tgcaatccaa tcaatagccc ggggatcatc gttactaccc agatgaacga
     2161 gagtatgaaa aacgtaaaac ccacgagaaa atcgatggaa agatttccgt aatcatcagt
     2221 cattgttatg agatcaacgc ccctgtggtt gcattaaata taagactcct ggctgaactt
     2281 ccggaagtga tagtcagggt gtagacccct ctttgcaatt caagatccca ccgttcagta
     2341 actcccggcg agggattatt cagaatcgcc gccctgtttt gtttgagcag attttcagga
     2401 gttacgatag tatcgagcga gatcttttct gaggggagcg ttacattctc aatggttgtc
     2461 cagcccgtta tgtcgtttat gagataaacc ggccctttct cgccatatac gccaaatatc
     2521 caggttctgg catttccgtg gtcgtccata tcacgggaaa gcatataatt tacatttttc
     2581 ctggtttcaa tatcgttggt tgattccgac cggtactcca ggaaattctg ttttgctgtc
     2641 tcgaaactga gacgtgaacc ttcactggca ggtgttatct gggtaaggcc ccccggccgg
     2701 gatgactggt cagctggtga ttgtgcaagg cacgcacaga ttaaaattaa tgcgaccagc
     2761 gtcatgatgg agaaaatggt ggggatattt ctgcgcatat tcttccttaa aggcttattt
     2821 agataagaac agatataaat attggtattc tttttagtat caatgaacga caaaaccgaa
     2881 caacgttcac gtgatacccc gctttatgta catccggcat catcacctca gttaatataa
     2941 gatactcatg ccggatgatt gggattacgg tacgatttta gtgataggta tctcaaggat
     3001 atcttcggca ccgcatgctt tgagttttgg gataagctgg ttgaccgtat tcttgggaac
     3061 aaccgtctgg atgctatagt agtcaatccc atggagacgg cttaccgttg gttttttcat
     3121 tgccgggagg gcagcaacaa ttttttccat gctgtgtgtg gggacattca tcgtcaggag
     3181 tactttattg cgtgcttcta ttacaccgaa gaggagtgtc tttatctctt cgatttcgcg
     3241 gcgtttatca ggatctgccc aactcgcttt gtttgctatg ataaccgtat aggactccat
     3301 aatctgtccg ataattttaa gcccgttttt acgcagggtc gtgccggtct ctgtcaggtc
     3361 aactaccacg tccatgagat ccggtacttt agcttctgat gccccgaatg aatggaataa
     3421 ctggaccggg attcccagcc gggcaaagta ttttttcgtg agttcagggt attccgtggt
     3481 cacccggctt tgcgggcgga tccggctgac atcatcgatc ggttcatccc ggtgaacagc
     3541 gacaacaatc ttcacatttc cttccccggt cttgctgtac gagagatttg cgacctcgac
     3601 tacatcggat ccagtttcat ttacccagtc caggccggat atacccacat caaagtatcc
     3661 tttctcaaca tacgtgggaa tttcctgggg gcgcaatatt tttatcttgc ctatccggga
     3721 atcggcgatc cggggattat aatcacggtc agtacgcctg acctcgagat cagcctcctt
     3781 gaaaagctgg agggtctggg cctcaaggct gccttttgga agtgcgatgg taatcatatc
     3841 acattcaatc gctgtaaaaa acgataaagg gcagggttgg aaattttttt aactaaacgt
     3901 atgaacaaca agcccactga gcagttttgg ttcaaaccac gtcgatttcg ggggcatgac
     3961 gccatctgca tctgcgatgg caagcacttt atcgaccttt accggctgca tggcaaacgc
     4021 tatgcggtac ctgccctcat taacaagttt ctcaagatcg gaaaccggac gagctccccc
     4081 gagatactgc aatcgtgcat ctccccgcgg atcggttatt cccagcattg tttcaagcac
     4141 gtgtttctgg agcacggcga catcaaggct catgaggggg gtcagacggg tatcaatcgg
     4201 acgcgtgcac tcgaaccact tccctgaaat gtacaggtga atcacgtgga atttttcagg
     4261 atctgaaatt cttgggggaa tgttgtaccc gttcccgttt accggcccgt attcctttac
     4321 tgaaaaacag tctttgagcc tgtcaaggaa catttcctct gagtaatccc ccaaatccgt
     4381 aagcagccgg ctgtacccgt gaatctttac ccggtcatga gcaaacatca cgcccatgaa
     4441 gtgtttcact tcaccttcgg caggtttccc ttctgcaatg cgatgatcaa cgacattcag
     4501 tgcagctttt gcccgatgat gaccgtcagc aatatagagc gatggtaccg atgagaagat
     4561 agtctcaagc tgatcaagga tttttgaatc catgatccgg tatatctggt gaattatccc
     4621 ggtatctgag gtaacttccg catccggtgt tttcgatcga tcggccagcg attcaataca
     4681 ggaaaaaatt tccttcctgt tcctgtacaa caggacaacc gggccgttgt gagcctgaac
     4741 tgcctcaata tgccgggtcc ggtcttcctc tttatcatac cgggtctgct catgccggcg
     4801 tatgtttctc ttccggtaat cgtcaacatc aaggcaacag caaagcccaa gaaatgtttc
     4861 tccatcctgt aaaaccctgt acaggtacat gccgggttcc ggatcctttt tcatacgtcc
     4921 actggcttca agcgcgatga aattgtcata cgcccgctgg tatacgcgct catcgtaagg
     4981 cgacagatcg ggcaattctg catccgctct gctcacccgc aaaaaagttt ctggattttt
     5041 tgcgatgatt gccctggcct catcagcggt cacgacatcg taaggaacag ccgcaatggc
     5101 aggtgctgca agacgatcgg gacgcatgcc tgaaaaacga tagatcgtga ccattttatt
     5161 cacctttttt ttaaccgcct gaagaaataa cctgtcctca ttgttgggtt tgctaccata
     5221 tattttccca cccacagtgt ttcggagcaa atgctatgta ctaaaaagat ctcattgatg
     5281 atactgctga gttgaggaga actggagtca aaaacgatga tccggccgcg tgatctttca
     5341 atgtacccgt taccccttat ccgtcgggct atgcctccag acatcgcagc gatcgttgag
     5401 atcgagaatg aatcatttat cgatccctgg gaacccgatg tgtttcttga agcgtttacc
     5461 ttttttcctt caacctattt tgtcgctgtt tgtgatggga ttgtcgcagg atttatcatc
     5521 ggagcacccg aaaacaccgg tgaaaatatc tatggccaca tatgcaattt cggggtaagt
     5581 cccgtttacc ggcaccgggg aatcgggagc ttactggtaa agcggatcga gcaccagttc
     5641 atgcttgaac tggcaaccgc cgtccagctg gaagtgcggg tatccaacac gaacgcgcag
     5701 aaattttacc gccgcctcgg gtaccgtgat gttttccgga tcgatgatta ttatttcaat
     5761 ggggaagatg ctctcgtaat gatgaaatgg ttcaggtttt aaccgcgccc gagtactggt
     5821 gagtgcagtc aaggtgatgg gcagcatggg ctccaggcag ggagagttcc ccggcaagaa
     5881 ccacggtcat aaggatcgac gccacatttt ttcgccatgt ttttcagttt gattacacaa
     5941 ggaaacgtat gcaacaacct cagcctcatc tgcggtattt ttgttcggtc acgtttctta
     6001 cctttctgct ctgtacaggt attgcaggtg ctgcgctcac ggatcatcct gttgttaatg
     6061 ggatctccgg cccggaccac ctgaatgact ggttcggccc gaccggttca tacacgttta
     6121 ccgctacggt caccggaggt acccctccct attcctacaa gtggatgaac cccccgggaa
     6181 tgaagatact ctttgaggga aagcagtacg gtactgttga aataccggct ccccagctcg
     6241 gcctgagtgg acccggtaac tacggtgtct ggctgaccgt aaccgactcc gcaggaaagg
     6301 acgcggtctg gttgcggcag ggcggatcgg gtaacagcaa ccagtttggg tacttcctga
     6361 caacagatgg aaaaacatgg tcgaaaaaga ccgagccggc cacgttccct cctccggttg
     6421 atgccccgca ggttattcag aaaacaagcg accagtgcgt ggattccgga gcacggttca
     6481 ccgggattca gggagacatc caggtgagca gcgaatgcaa tggggactat tccaagggca
     6541 tatggcatct tgcaaaacgc gaaattaccc tgtacggggg cgatcacatc aggaccggtg
     6601 aggacaccac gggatatctc ggttttcagg acatgagcac atttattctc aaagcagaat
     6661 cagagatagt cctgcttgtg ccggaaaata aagacagcaa gctgaagctg gtggccggca
     6721 acatctggac gaatgttaag aagatggtaa aagacggttc catggagatc gatatggacc
     6781 aggctgtttt aggaacaaag gggaccacgt tcgtctgctc ttcagatggc aaaacgtcaa
     6841 ccatgcagat cattgaaggt acagttgagg taaccggtaa ggcggataaa aagaagatcc
     6901 tggtcacggg tggccagcag gtgacggcaa ccagcaaggg tcttggaaca cctgtggctt
     6961 tcgatgcacc tgctgcacag gcagactggg atgcagtaaa agcaaaggca ctctccggaa
     7021 caccggcacc cgcgccagca acgactaaga agtccggtct cgaatccctg cttgtccttg
     7081 cagcttttgg tatcgttgtg gcaggtatag tattcagacg cgaataggtg ggataaacca
     7141 gaaactgcga tgcccattca taatggcacc gttttttaaa aaagatcgag cgcttcttat
     7201 cagtgcttcg aaaaaaacga gagaaaaaaa ttatcctctt cccagttgct ggtgtgcccg
     7261 ggcaaggtgc tgtgcagcaa gcgcaccgag cagggagagt tccccggcca ggacgccagc
     7321 accgatgatc tctgcgagtt tcctggcatt ggtcccgggg ggatccccgc ctccggcaac
     7381 gccgagtatc cggaggcatt cctgctgtgt ttccagaccg gtccctcccc cgactgtgcc
     7441 cacgggcagt gaagggagcg tgaccgagac atatgccccg gcatcagtaa gatcgaccgt
     7501 tgtgatacag gtactgccgt caattgcatg ggctgcgtcc tgcccgcagg caatgaacat
     7561 ggttacgatc gcgagcgcaa a
//
LOCUS       DASRLV010000130         8785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_287800,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000130 DASRLV010000000
VERSION     DASRLV010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 8785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8785
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287800"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFC43_05110"
     CDS             <1..342
                     /locus_tag="VFC43_05110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HZK30599.1"
                     /translation="VVLYKKSWKKASGSGVSDINRKEFMDWTNGLWTFNGERKKKTGH
                     PAPFPVELPLRCMKLFSFVGDTVLDPFMGSGSTLVAAAKCNRRGIGIEIDPGYCEIAV
                     DRIEIEGEQKD"
     gene            457..1368
                     /locus_tag="VFC43_05115"
     CDS             457..1368
                     /locus_tag="VFC43_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HZK30600.1"
                     /translation="MAGTDIFIAIASDRTSGLRRILGEIDTSIFSGAEIALKANYNSA
                     DLPPASTHIDTLDALCTSILAEKPKELTLAERSGMGNTRTVLEERGVISLAKKRGFSA
                     VVLEELDRTGWREIQSPGLHWSRGFFIASIFAQADRVIQTCCLKTHRFGGHFTLSLKN
                     SVGLIAKRVPGVNYDFMGELHSSPHQRLMIAEINKFYRTDLIVMDAVDGFSRGGPDKG
                     KIIIPGIIIAGSDRVAIDAVGVALLRSYGTMHDVTEGRIFEQEQIARAADLGIGVASA
                     AEIHLVPLDTGAELIAARIQEQLDSDR"
     gene            1480..1928
                     /locus_tag="VFC43_05120"
                     /pseudo
     CDS             1480..1928
                     /locus_tag="VFC43_05120"
                     /inference="COORDINATES: protein motif:HMM:NF039354.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
     gene            2013..2573
                     /locus_tag="VFC43_05125"
     CDS             2013..2573
                     /locus_tag="VFC43_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HZK30601.1"
                     /translation="MTIESAAVKSVAGLMALSARTAPKAVGLDSIKIEVLSGKEQEKL
                     ARQMIKQGTELKIDFFRINGEQVKVSDATLLIGVEGHIPLGVNCGGCGHSSCAEMVKA
                     LRAQKTKTAMYPGPNCVLKITDLGIAVGSAVKTASIHNVDNRIMFSAGVIALQMGMLK
                     GCSVAYGIPLKASGKNIFWDMKFTDH"
     gene            2892..5003
                     /gene="ppk1"
                     /locus_tag="VFC43_05130"
     CDS             2892..5003
                     /gene="ppk1"
                     /locus_tag="VFC43_05130"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HZK30602.1"
                     /translation="MMNTQQIPVDNPAFYINRELSWIRFNRHVLDEARDKSHPLLERV
                     KFLAIFANNLDEFFMIRVSGLQRQVLKGVLKLPADGMTPSQQLDTVRQMLLPELEAQS
                     DTWCDEILPQLDAAGIHIHLYSDLTDEQKKCMHTFFVNEVFPVLTPLAFDSAHPFPFI
                     SNLSLNLAIIVRDAAKKEYFARVKVPTNLFSRLVRVPEPSGNGRSDDSDVHFVYLEEI
                     IAAHLDMLFPGLEVMASFPFRITRDADLEIEVDEASDLLTTVEEIMEQRAKGKPVRIE
                     LDCSMHDNICHMLEKKLGITSDMLFPVSRPVGMADLMQLMSLDRPDLKDTPFLPSISS
                     DVAEGKDIFSAIRRRDILLYHPYDSFTPVVNFIRQAAHDPDVLAIKITLYRVGSNSPL
                     VNALMEARENGKAVAALIELKARFDEENNIGWARALEHAGVHVVYGVVGLKVHAKLCM
                     VVRREKEGIRSYVHLGTGNYNATTSRIYTDFGFFTCDRQIGEDVTNLFNFLTGYARIE
                     KYQKLLVAPVTLRNGIIDRIDREIVAHSEHGDGHLIFKMNALVDPLCIAALYRASQAG
                     VKVELQVRGICCLRPGITGISENITVSTIVGRFLEHARIYYFHNGGSEEVFLGSADLM
                     PRNLDRRVEVLFPVENQSLRNNIITSILPVQLNDTIKVRLLNSDGSYERRRPSGTIPP
                     LNAQLWLVDHRGMIWYNDDKQ"
     gene            4987..6597
                     /locus_tag="VFC43_05135"
     CDS             4987..6597
                     /locus_tag="VFC43_05135"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HZK30603.1"
                     /translation="MTISSEMPSIRAECVFGTQRLIPLLEAFSKEIEGVKTAQDIEHI
                     HRMRVASRRLRAALPLFQSCIPERKYHQWLREIQKITQALGDARDTDVQIAFITKIIK
                     KRGGGKQDNQTDISSSVRDKGDVETVLLLQLQKKRSKLQADVVSDLEKLEKSGIIDEM
                     RTFFLTQTSGINKRKKSPLFGIPPVAALRISRRLKTLLSYERWVHNMDAVAEHHAMRI
                     AAKRLRYTIEIYAPLYRLELKKYLSRVKKIQEILGDLHDCDVWIDTVMVMLLKERSTY
                     HVKNSSINRQVNRVSGYRHFLSEREKERKRLFLRYVRYWDSLTRAGIWDELRRTLNTG
                     RKQKFILPVVSSEEAIRSSVSILASQYPEGVPHTRNVTALAMRMFDDLVSLHRMGARE
                     RFLLECACSLHDIGWKYGQKGHAKHSADMIISDEQLLLDINERNIIALVSQAHRGNAS
                     PESKDIFSLLTPEERENALMLASLIRIADGLDYSHNGSIQSIRCTINPEDVMFEISTV
                     HDAASELERARLKGDLFVRVFKRPLVIR"
     gene            6594..8171
                     /locus_tag="VFC43_05140"
     CDS             6594..8171
                     /locus_tag="VFC43_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HZK30604.1"
                     /translation="MTIKKIGLNGRVVSFIDIGTNSVRLLVVRLNPNHSYSILSQQKQ
                     QVRLGEGEFEDEEILPEATERLVIVCKKLTELARTFNTDEFVAVATSAMREATNQQEI
                     LHLLRQEALLDVRVISGHEEARLIYLGVSRGIHLAERQAFFIDIGGGSTEIAVGGDQN
                     YHYLESFRLGAIRLANLYLSGVVTEPVTQDQYKKVQQHVKDTIIHSVKKIRSQKPDIA
                     VGSSGTIVNLFEIAQKAFHQNGSSEENVLIYKDLRKIIDLLCSLPLEQRRKIPGMNPE
                     RADIIIHGAAILDTFMKELSLESIAVTSRGLQDGLLVDYLSRMDEFPLLGELSPRQRS
                     VLQLGRSCGINEVHARTVTALVLELFDSSKAVNLHNFGDWERELLEYATFLHDIGSFI
                     SYTNHHAHSYYIIKNSELLGFDQKEVNFMANIARFHRKKTPKKKDPEILELDIRERET
                     LRVLTTFIRLGESLDRSHTALIQHVRFSNVDRDAVTLEVVARGDCQLEIWGVEAERKA
                     FEKIFGKKLVLERIQTT"
     gene            8540..>8785
                     /locus_tag="VFC43_05145"
     CDS             8540..>8785
                     /locus_tag="VFC43_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZK30605.1"
                     /translation="MQYRFANRLSHAPKSFLNELFRVSSDPSVISFAGGLPSSALIDT
                     EGLKKATFQVMEEDPSTALQYTTTDGFLPLREFIAERY"
BASE COUNT         2399 a         2019 c         2149 g         2218 t
ORIGIN      
        1 gtggtgctct ataaaaaatc atggaagaag gcgagcggtt caggagtgtc ggatataaac
       61 cggaaagagt tcatggactg gactaacggg ctatggacgt ttaacggtga aaggaagaaa
      121 aaaaccggtc atcctgcacc attccctgtc gaactaccac tccggtgcat gaaacttttc
      181 tcatttgtcg gggataccgt attggaccca tttatgggta gtggttcaac gctggttgcc
      241 gctgcaaaat gcaaccgtag aggtattggg atcgaaattg atcccgggta ctgtgaaatt
      301 gcggttgacc gtatagagat agaaggggag caaaaggatt gacaagcggt cctgttgcca
      361 gggtctgttc tgcaatggaa aggtcctttt ttgataggga atcgcgtagg gaaagatttc
      421 atattccctg gagagaaccg atacacggta atactcatgg caggtaccga tatatttatt
      481 gccattgcat ctgatcgcac gagcggattg cggcgtattc tgggagaaat tgacacatca
      541 atcttttcgg gagctgaaat tgccctcaag gcaaattaca acagcgcaga cctgcctccg
      601 gcatccaccc atattgatac cctggacgcg ctctgtacgt ccatccttgc agaaaaaccc
      661 aaagaactca cccttgctga acgcagcggt atgggaaata cacgtaccgt tctggaagag
      721 agaggcgtga tttcacttgc aaagaaacga gggttttctg cagtggtcct tgaagaactc
      781 gatcgaaccg gatggagaga aatacaatct cccggcctcc actggtcccg cggctttttt
      841 atcgcaagta tttttgcaca ggctgaccgt gtcatacaga cctgttgtct caagactcac
      901 cggttcgggg ggcatttcac gttatcactg aaaaattcag ttggtctcat tgcaaaacgg
      961 gtcccagggg tgaactacga ttttatgggg gaactgcata gttcgcctca ccagaggctg
     1021 atgattgcag agatcaacaa attctaccgc acggacctta tcgtgatgga tgctgtggac
     1081 gggttctcca ggggggggcc ggacaagggt aagatcatca tcccggggat catcatagcc
     1141 ggatccgatc gggttgccat agatgcggtg ggagttgccc tgctccgctc gtacgggacc
     1201 atgcatgatg tcacagaagg aagaatcttt gaacaggaac agatcgccag agcggcagat
     1261 ttaggaatcg gtgttgcatc tgcagcagaa atccatcttg tcccactcga cacaggggct
     1321 gagttaatcg cggcccggat ccaggaacaa ctggatagtg accggtgatg gaaaccagtc
     1381 gtcagatttt tgcccttcaa aagaacattt aggtggatga aaccagagca ggaagatgtg
     1441 tcacacgagc tgaccgatgc agccaagaac tgggtcgcca tggatggtct ctggttccag
     1501 gcggttgagg aggcttatgg gatggaggct gcccttgatc tcgacagatt ggtctgggag
     1561 cagtttgcct caattgaagc cgggcggata aaggagcgac tttccctacc ggaaaacggc
     1621 gggctggacg cactggagat cgccttaaaa aacaggctcg tttctttcct taacactctg
     1681 gagatccggc gccctgataa aaataccctg atcgtcacga ctaagacctg caggtacagt
     1741 cagcccgcga aaggaaagga ttgcccctgt ttccctgcaa atcggtcggt cttatagaat
     1801 tccccgtatt tgcacggacc attgattcac ggattgtaac ccaatgtctg tcatgtccgc
     1861 cacaatccca gcccgggacc ccgtattgtt cctggaaatt taccatcgag gggacgagtg
     1921 aaatctgacg gagatttcct ctcccgatgg catggatcga tcctgcctta tatatccgct
     1981 cattttttga cctgacctga taacatacca gtatgactat tgaatctgca gcagtcaaat
     2041 ccgtagcggg tcttatggct ctctctgccc ggacagcacc gaaggctgtc gggctggact
     2101 caattaaaat tgaagtatta tcaggcaaag agcaggaaaa actcgccaga cagatgataa
     2161 aacagggaac agagttaaag attgatttct tccgtatcaa tggtgagcag gtcaaagtga
     2221 gcgacgccac tctcttaata ggtgttgaag ggcacattcc gctcggcgtc aactgcgggg
     2281 ggtgcgggca ttcttcctgt gcagagatgg taaaagcgct cagggcccaa aagacgaaaa
     2341 cagccatgta tcccgggcca aactgcgtcc tgaagattac tgatcttggt attgctgttg
     2401 gttcagcagt taaaactgcc agcattcaca acgttgataa ccggatcatg ttcagtgccg
     2461 gtgtcattgc actgcaaatg ggcatgctca aaggatgcag cgttgcgtat ggtatcccgc
     2521 tcaaagcctc cggcaagaac atcttctggg acatgaagtt cacggatcac tgatatttat
     2581 taccgctcac acaaacctgc ggttatggac aacaggagca ataagataca aattagagaa
     2641 gtcgatcaac ggaatagaga aatgcctgca tttttatata gattcaataa aattaattca
     2701 atagtcagag aaattagtgc atactaatat cataaaaaaa gaccttaaaa gaagcacata
     2761 cgaaaaaata tgaaaatgcg cccggataat cctctgcaaa aataggattt gaacaaaaac
     2821 ggctccggtg aattaatgta ttcatctttc gtccgttctg agggttcggg caatatgcgt
     2881 actggatact tatgatgaac acgcaacaaa ttcctgttga caatcctgcg ttctatatca
     2941 accgggaact aagctggata cggttcaacc gccatgttct tgatgaggca cgggataaaa
     3001 gccacccttt gttagagcgg gtaaagtttc ttgctatttt tgcaaataac ctcgacgagt
     3061 tctttatgat cagggtatca gggctgcagc ggcaggtatt aaaaggggta ttaaaattac
     3121 cggcggatgg gatgacaccg tcgcagcagc tggatacggt ccgccagatg ctcctgcccg
     3181 aactcgaggc tcagtctgat acatggtgtg atgaaattct gccccagctg gatgctgccg
     3241 gtattcacat acacctttat tccgatctta ctgatgagca aaaaaaatgt atgcacacat
     3301 tctttgtcaa tgaggtcttt ccggtcttaa caccccttgc ctttgatagt gcccacccgt
     3361 ttccctttat ctcaaatctc tcactcaacc ttgccatcat cgtgcgggat gcggcaaaaa
     3421 aagaatattt tgcccgggta aaggtaccga ccaacctgtt cagtcgtctt gtacgtgtcc
     3481 ctgaaccatc cggaaacggt cgatcggatg attccgatgt tcattttgtt tatctcgagg
     3541 agatcattgc tgcacacctt gacatgctct ttccaggtct cgaggtcatg gcatcattcc
     3601 cgttccgaat caccagagac gcagatctgg agattgaggt cgatgaagcc tcagaccttc
     3661 tgaccacagt agaagaaatc atggaacagc gggctaaagg aaagccggta cgaatcgaac
     3721 ttgattgttc catgcacgat aatatctgcc atatgctcga gaagaaactg ggaattacct
     3781 cagatatgct gtttccagtc agccgcccgg tcgggatggc agaccttatg cagctgatgt
     3841 ctctcgatcg ccctgacctc aaggatactc ctttccttcc atcaatttct tctgacgttg
     3901 ccgagggtaa ggacattttt tctgctatca gacgtcggga tatcctgctc tatcatccgt
     3961 atgatagttt cacccccgtg gtaaatttta tacggcaggc agcgcatgac ccggatgttc
     4021 ttgccataaa aattaccctt taccgtgtcg gctcaaactc accgttggta aacgccctga
     4081 tggaggcccg cgaaaatggt aaagccgttg ccgcactcat tgaactcaaa gccaggtttg
     4141 acgaagagaa caatatcgga tgggcacgag ctcttgaaca tgccggagtt catgtagtct
     4201 acggcgttgt cggactaaaa gtccacgcaa aactctgcat ggtagtgcga agggaaaaag
     4261 aggggatccg tagttatgtt cacctgggca ccggaaatta caatgctacg acaagccgta
     4321 tctatactga ttttggcttt ttcacgtgtg accggcagat tggtgaggat gttacgaacc
     4381 tgttcaattt cctcacggga tacgcccgga ttgaaaaata ccagaaactg ctggtagcac
     4441 ccgtcactct ccgtaatggg atcatcgatc gcattgaccg ggaaattgtt gctcattcag
     4501 aacacgggga tgggcacctc attttcaaga tgaatgcgct cgtagatcct ctttgtatcg
     4561 cagcactcta tcgcgcctcg caggccgggg ttaaagttga actccaggta agggggatct
     4621 gttgcctgcg tccgggaatt accgggatta gtgaaaatat caccgtaagt actattgttg
     4681 gcaggttcct tgaacatgct cggatctatt acttccacaa cgggggatca gaagaagttt
     4741 tcctcggaag tgctgatctt atgccacgaa atcttgatcg gagggtggag gttttatttc
     4801 ctgtggaaaa ccagtcactt cgaaacaata taattacatc aattttaccg gtgcagctga
     4861 acgatacgat taaggttcgt ttgctgaaca gtgatggttc ttacgagcgc cgcagaccat
     4921 caggtaccat tccgcccctt aatgcgcagt tatggcttgt tgatcaccgt gggatgatct
     4981 ggtataatga cgataagcag tgaaatgcct tcaattcggg cagaatgcgt ttttggtacg
     5041 cagaggctta ttccactcct tgaggcattt tcaaaagaga ttgagggggt aaaaacagca
     5101 caggatatag aacatatcca tagaatgcgg gtagcgtcaa ggagacttcg tgcggccctc
     5161 ccactattcc agtcgtgcat ccctgagaga aaataccatc agtggttgcg ggaaatacaa
     5221 aaaataaccc aggcactggg agatgcccgt gataccgatg ttcagattgc atttatcaca
     5281 aaaatcataa aaaagcgggg aggtggaaag caggacaatc aaacggatat cagctcttca
     5341 gtccgggata agggagatgt ggaaacagtt cttctcttgc aattgcagaa aaaacgttca
     5401 aaactccagg ctgatgtggt ctccgatctg gaaaaactgg aaaagagtgg tattattgat
     5461 gagatgagaa cgttttttct tacgcagaca tcggggatta ataaacggaa aaaatcgccg
     5521 ctgtttggga ttcccccggt tgcggcgtta cggatatccc gtcgcctgaa gacattgctc
     5581 tcctatgagc gatgggtgca caatatggat gcggttgctg aacatcatgc aatgaggatt
     5641 gcggcaaaaa gactccggta cactatagag atttatgctc ccttgtacag gcttgaactc
     5701 aaaaaatatc tttcccgggt aaaaaaaata caggaaatac ttggggatct gcatgactgt
     5761 gatgtctgga ttgacacagt tatggtcatg ctcttgaagg aacgctcaac ataccatgtg
     5821 aagaattcat caataaaccg acaggtaaac agagtatctg gttaccggca ttttctatcc
     5881 gaacgagaga aagagagaaa acgacttttt ctgaggtatg taagatactg ggattcactt
     5941 acccgggccg gtatctggga tgaactccgc agaactctga ataccggaag aaaacaaaaa
     6001 ttcatccttc ccgtagtttc ttcagaggag gcgatccgct cttcagtttc cattcttgcc
     6061 agtcaatatc cggagggggt accgcatacc cgtaacgtga ctgccctcgc catgaggatg
     6121 ttcgatgatc tcgtatcgct tcaccgcatg ggtgcacggg agcggttcct tcttgaatgc
     6181 gcctgctcgc ttcatgatat cggctggaaa tacggccaaa aagggcatgc gaaacacagc
     6241 gctgatatga taatatctga tgaacaactt ctcctcgata taaatgagcg aaacattatt
     6301 gcgctggttt cgcaggctca ccggggaaat gccagtcccg aatccaagga tattttctct
     6361 ctactgacac cagaggagcg tgaaaatgct ttgatgctgg cttcgttgat ccggattgct
     6421 gacggacttg attattccca taacggatca attcaatcca ttcgctgtac cataaaccct
     6481 gaagatgtca tgtttgaaat ttcgacagtc cacgatgcag cttcagagtt ggagcgtgcc
     6541 cgtctcaagg gagatctttt tgtcagggta tttaaacggc cactggtgat ccgttgacaa
     6601 tcaaaaaaat aggcctgaat ggacgtgtgg tatcctttat cgatataggg acgaattctg
     6661 tccggctcct cgtcgtgaga ctcaacccga atcattcata cagtattctc tcgcagcaaa
     6721 aacaacaggt acggctcggg gagggagagt ttgaggatga agagatcctt cccgaggcaa
     6781 ctgaacgcct ggttatcgta tgtaaaaaat taactgaact tgcccgtact tttaataccg
     6841 atgaatttgt tgctgttgcg acctctgcca tgcgggaagc aaccaatcag caggaaatcc
     6901 tgcatctttt acggcaggag gcccttctgg atgtccgcgt aatatccggg catgaagaag
     6961 cacgtcttat ctatcttggc gtttcaagag ggatccacct tgcggagcga caggcttttt
     7021 ttattgatat cggtggggga agcactgaaa tcgcagtagg tggcgatcag aactatcatt
     7081 accttgagag cttcagactg ggggctatcc gccttgcaaa cctgtattta tccggtgtgg
     7141 taaccgaacc ggttacgcag gatcagtata aaaaagtcca gcagcatgtt aaagatacaa
     7201 ttattcattc tgtcaaaaag atacgctcac aaaaacccga tatcgccgta ggaagttccg
     7261 gcactatcgt gaatcttttt gaaattgcac aaaaagcctt tcaccagaac ggcagttcag
     7321 aggaaaatgt gctgatttac aaagatttgc ggaaaatcat tgatctgctg tgttcgctac
     7381 ctcttgaaca gcgcaggaaa ataccgggga tgaatcccga gcgtgcggat atcatcatac
     7441 acggtgcagc cattcttgat acgttcatga aagaactgtc acttgagtcc atcgcggtaa
     7501 ccagccgtgg attacaagac ggtctcctgg tcgattacct gtcgcggatg gatgagttcc
     7561 ccctccttgg cgaactctca ccgcggcagc gaagtgttct ccaactgggg aggtcctgcg
     7621 ggatcaatga ggtgcacgca cgaacggtta ccgccctcgt cctggagttg ttcgattctt
     7681 caaaagcggt taaccttcat aattttggcg actgggagcg tgaactgctt gaatatgcaa
     7741 ccttcctcca tgatatagga tccttcattt cctataccaa tcatcatgca cattcctatt
     7801 atattataaa aaattcagag cttctcggtt tcgatcaaaa agaagtaaat ttcatggcta
     7861 atattgcacg gtttcaccgg aaaaaaaccc cgaagaaaaa agatccagaa atccttgagc
     7921 ttgatatccg cgaacgcgaa accctccggg tactgacaac attcatccgg ctcggagaaa
     7981 gtcttgaccg aagtcacaca gcactgattc agcatgtcag gttctcaaat gttgaccgtg
     8041 acgcggtaac tctcgaagtg gttgcccgcg gggactgcca actggaaata tggggtgttg
     8101 aagccgagag aaaagcattt gaaaaaatct ttgggaaaaa attagttctg gaaagaatcc
     8161 agacgacata atcaccataa attccggtgt caaggagagg actaaaaatt ctctgtcttt
     8221 ttattattat acacgtgtcc aatacccatc tataatgcgg aatgtaactt tgacctgcag
     8281 gcgagaggag gcaggaatcc tgtgcatgtc cgaatgatcc ggtcatcacg gtacgatatc
     8341 ccgtaacctg ctaacaccaa acaactatta ccattcctga atataccgtg ggagattatc
     8401 ctcaatacct gaagtatccg ggatagtact ataaattccg tacagcaatc tgccgataat
     8461 atcataggtt ttttctctca tttatgcata cacaaactat cagatcctga tatagggagg
     8521 atttcagagg agtaatccga tgcagtaccg ttttgccaac aggctctcgc atgccccgaa
     8581 atcatttctt aatgagcttt tcagggtttc ttcagatcct tcggttatat catttgcggg
     8641 aggtcttcca tcgtctgccc tgatagatac tgaaggtcta aagaaggcca cgttccaggt
     8701 catggaagag gatccctcca ctgcactcca gtatactacc acagatggct ttcttccttt
     8761 acgtgagttc attgcagaaa ggtac
//
LOCUS       DASRLV010000131         7854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_451167,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000131 DASRLV010000000
VERSION     DASRLV010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 7854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7854
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451167"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /gene="cfbD"
                     /locus_tag="VFC43_05150"
     CDS             complement(<1..910)
                     /gene="cfbD"
                     /locus_tag="VFC43_05150"
                     /EC_number="6.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide reductive
                     cyclase catalytic subunit"
                     /protein_id="HZK30606.1"
                     /translation="MKYMHPRPSSIVAALYTARDLKVDVAILHGPSGCSFKHARLLEE
                     DGMRVLTTSLADNEFIFGGQKPLEDVLRYAEDHFSPERMAVIGTCVAMIIGEDLQSAI
                     DGAGVTTPTIAVDIHAGYLENIAGVLSTLEAACEAGWISHDELTRQRFLMEKANEVER
                     LRGAASLPYIEPSRGDLKHIAAQRLLELARSGKKGVAVLNAKKETAYMFADELVALHD
                     SCPDADITYIANLDQRGLPKTRLDADRIFKEMTSRGLNIEVIGALDEYGANGDLLGRK
                     LLEIKPAFALISGVPHAIPPEYTRGIE"
     gene            complement(921..2141)
                     /gene="cfbE"
                     /locus_tag="VFC43_05155"
     CDS             complement(921..2141)
                     /gene="cfbE"
                     /locus_tag="VFC43_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F430 synthase"
                     /protein_id="HZK30607.1"
                     /translation="MHILVLDTIHGGKKIGEAFAARGDQVDCVDVYRGESTIDVKTAQ
                     AGTYDLVVAPVHLDPDHPLVNFSGVPVISHHEAIRRLLGQNIPEPMVEITGARGKTTT
                     AHALASLMEGPGILHTSTGTYRFPEKNRISKCSITPGSVLAAVSMAKGINGWLIVEES
                     LGISCAGTLGIITSPEDYSFAAGKKTALLTKVSSAKNCAHLLLAENILVPSVEKISHI
                     EDIATCEGTECSLSLAGKTCRFSNPLLELAGYRTSLMLAAAAAMILGIDPEPLSSFSA
                     IAGRMSESYEQGVLVVDNANSGTNLASTVKAAQYARSKSGTEAVTLVIGQVEGDGAVC
                     EGFAIDQIQAAINQISPTHVVWVGTFPKPETPEFRIPQRLISSYEVTLEGAFISALRM
                     TKQGSIVLAVKTWR"
     gene            complement(2152..2553)
                     /gene="cfbA"
                     /locus_tag="VFC43_05160"
     CDS             complement(2152..2553)
                     /gene="cfbA"
                     /locus_tag="VFC43_05160"
                     /EC_number="4.99.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin nickelochelatase"
                     /protein_id="HZK30608.1"
                     /translation="MGKKGMLLVGHGSTMPYNQELVEKTAAIIKGQNTDFIVKCGFMN
                     MNTPTIKDSLNEFKHEPIDALVVVPLFLAKGVHIEKDIPGEIGLAEGTKKGVFLLNGR
                     SIPLVYDDPIGSDPLLANLMIKNATKALSLL"
     gene            2685..3824
                     /locus_tag="VFC43_05165"
     CDS             2685..3824
                     /locus_tag="VFC43_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 9 domain-containing
                     protein"
                     /protein_id="HZK30609.1"
                     /translation="MMDAFERFGLLINDRIVRTPVAIASMAGWVDAAYVLERADHIGA
                     AFIGGYSIDQPTIEAARIISDEGKRKEFLYDDPVEELKKQMALLEKSSVVLGLNLRGS
                     TPASFSAVARSLGENVVYEIDAHCRQPAMVAAGCGEHYLKKPGDLMAVVRALKSEGVT
                     VSVKIRAGISADDRLLARKLWEAGADILHIDLMDFGSPKIRQIRNVCPLMLIANNSIN
                     TFERMREMLSHGADIVSLARKSDIRTLSGLDAAITRYATEEGWYNSPKQLCRGGDVRA
                     LAFCCMPVKECPLIPTLSRIGMPKEEYIRMKLDTVKGTPLEEGNQTCFGSLAWCCKTS
                     SPCMFRDMTLKQRGLSKKEYMRLKRELSDTIMSRVFHDVPSDESS"
     gene            3796..4617
                     /locus_tag="VFC43_05170"
     CDS             3796..4617
                     /locus_tag="VFC43_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HZK30610.1"
                     /translation="MTCLLMRAAERAQLAMMLEVCAYPKPGNVDRCHDYPDTRLEHFL
                     ASTIFARQAFEESETGTGRIGEIIKHAVRDTNCHEGGNTHFGAFLLLVPLIYGKGIPG
                     ALMAIEKTDVSDAVAFYKAFAMTSVRMNAADELDVHDPHTLTMIRDREMTLLDIMQHS
                     AANDMVAREWVTGFRLTRRGADLLKNFGPGRQAIVNTFLTLLASEPDTFIIKKHGTEI
                     AEETMTSAREVLDGSYTIEDFDADCIERDINPGSTADIVIASIFIALGEGWNWDY"
     gene            4599..5156
                     /locus_tag="VFC43_05175"
     CDS             4599..5156
                     /locus_tag="VFC43_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 domain-containing protein"
                     /protein_id="HZK30611.1"
                     /translation="MELGLLKSGINEVIATTVFNAAPMGIHYREGNASMVIFSGSNTA
                     ENIERDGWVVANFVHDPVVYVRTAFEDLPRIAFIEEPVANKTMMRLAGADAWAAFTAT
                     IVRKTNESMMVHLRLEKEIFEEVTLHPVNRGFNSIIDATVHATRYRDNRDPELKRLID
                     HHVSIVRKCGGKREIEALDLLLKYI"
     gene            complement(5442..6437)
                     /gene="hypE"
                     /locus_tag="VFC43_05180"
     CDS             complement(5442..6437)
                     /gene="hypE"
                     /locus_tag="VFC43_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HZK30612.1"
                     /translation="MKVNMMHGAGGEVMGELLQTLTKFRHNNAGGIGLEAMDDGAVIP
                     LNGENLVFTTDSHVVRPIFFPGGDIGRISVCGTINDLAMMGGRPIALSCGMIIEEGFE
                     IDNLERIVASMDEALGEAGANLVTGDTKVMEKGALDGIVINTSGIGIAKKIVRDNGLV
                     PGDVIIVSGTLGDHGIAIMAHREGFDLGEQIKSDVAPLWGMVQKVLDAGIIHAMKDPT
                     RGGFASAINEMARKSGVGIRVREESLPVRKSVRSAAAMLGIDPLEVANEGKVVMGVPP
                     SCADAVLAALHSHKYGKDAAVVGTVTKGSHVIMETLIGGERYIEPPIGDPVPRVC"
     gene            complement(6439..6777)
                     /locus_tag="VFC43_05185"
     CDS             complement(6439..6777)
                     /locus_tag="VFC43_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HZK30613.1"
                     /translation="MHEYSIAYDLYATTRKAAIENNASQVKKVSVEVGKMAMVNPEQV
                     IFLFNTIKEDDPLFSAAILECNEMPPFTKCSCGYSGEEIFVCPQCGALPELVKGREIV
                     VTKVEIEVDD"
     gene            complement(6777..7001)
                     /locus_tag="VFC43_05190"
     CDS             complement(6777..7001)
                     /locus_tag="VFC43_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HZK30614.1"
                     /translation="MCVAVPAEVLEIKEGNIGLVDYGDLRQEVRLDLVDVKIGEFVLV
                     HVGFAIQRLSREEGLETREIFKQVYAALED"
     gene            7169..7582
                     /locus_tag="VFC43_05195"
     CDS             7169..7582
                     /locus_tag="VFC43_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30615.1"
                     /translation="MQKKIAVSGSVAGILILILMVVINVMVNMVIPADISRYGGMRAA
                     DDPVMILFFFYPFVVAFAAAIIFDVVKDCLKGTPVQKGLMFGALLLVMMTIPSLYVMI
                     TSMTWPIDFYISTASWEIISFPLMGMLFTKIWELS"
     gene            complement(7642..7726)
                     /locus_tag="VFC43_05200"
     tRNA            complement(7642..7726)
                     /locus_tag="VFC43_05200"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7690..7692),aa:Ser,seq:tga)
BASE COUNT         1955 a         1957 c         1881 g         2061 t
ORIGIN      
        1 actcaatacc acgtgtatat tccggaggta ttgcatgagg gacaccgctt atgagtgcga
       61 atgcaggttt aatttccaat aatttcctgc caagcagatc gccattcgcc ccgtactcgt
      121 ccagtgcacc gataacctca atattcaaac cacggctggt catttcttta aaaatccgat
      181 ccgcatccag ccgggttttc ggcagtccgc gctgatccag attggcaata taggtaatat
      241 cggcatccgg gcaggaatcg tggagggcta ccagttcatc agcgaacatg taggcagtct
      301 ccttctttgc attcaggaca gcaactcctt ttttcccgct ccgggcgagt tcgaggagtc
      361 gttgtgctgc gatgtgtttc agatcccccc gtgagggttc aatatagggc aggctcgctg
      421 cacccctcag ccgttccact tcatttgcct tctccatcag gaaccgctgg cgggtgagtt
      481 cgtcatggga gatccatccg gcttcgcacg cagcttcaag tgtcgaaaga acaccggcga
      541 tattctcgag atagcccgca tggatatcga ctgcgattgt gggagttgtc acccctgctc
      601 catcaattgc tgactggaga tcttcgccaa taatcatagc cacacaggtc cctatcactg
      661 ccattctctc aggagaaaaa tgatcttcag cataacgcag gacatcttcg agcggtttct
      721 ggccgccaaa aataaattca ttgtctgcaa gtgatgttgt gagcactcgc ataccatctt
      781 cctccagcag ccgggcatgt ttaaatgaac agccggaagg accatggagg atagcaacat
      841 ctaccttgag atcacgtgct gtatagagtg ctgcaacaat tgagctgggg cgcgggtgca
      901 tgtatttcat ggtaattcag ttatctccag gtctttacgg caagcacaat ggatccttgt
      961 tttgtcatac gcagagctga gataaaggcc ccttcaagcg tgacttcata cgacgagatg
     1021 agccgttgcg gtatcctgaa ctctggtgtt tcaggctttg ggaatgttcc cacccacacg
     1081 acatgggttg gactgatctg attgattgca gcctggatct ggtcgatcgc aaacccttcg
     1141 cagacagctc cgtccccttc aacctggccg ataacaagtg ttacggcctc tgttccggat
     1201 ttgctgcggg cgtattgtgc tgccttgacc gtacttgcca gattcgttcc actgtttgca
     1261 ttgtccacga caagaactcc ctgttcatac gactctgaca ttctgccagc tatcgcgctg
     1321 aatgaggata aaggctcagg gtctattccc agtatcattg cagcagccgc agcaagcatc
     1381 agggatgtgc ggtatccggc aagttcaagg agaggatttg aaaacctgca ggtttttcct
     1441 gcaagcgaaa gggagcactc tgtaccttca caggttgcaa tatcttcgat atgtgaaatt
     1501 ttttcaacag acggtacgag aatattttca gccagaagaa ggtgagcaca gttttttgcc
     1561 gaggagactt ttgtcaggag cgccgttttt tttcccgcgg caaaagaata atcttcaggg
     1621 gaagtgataa tccccagtgt tcctgcgcag ctgatcccga gagattcttc tacaatcagc
     1681 cacccattga tcccttttgc catactgaca gcagcgagca ccgatcccgg tgtaatgctg
     1741 cattttgaga tcctgttctt ttccgggaac cggtatgtcc ctgtcgacgt gtgcaggatc
     1801 cctggtcctt ccattagtga agcgagcgca tgtgctgttg ttgtctttcc cctggcgccg
     1861 gtgatctcca ccatgggctc aggtatattc tgacccagca gccggcgtat agcttcatgg
     1921 tgggaaatta ccggaactcc ggaaaagttt acaaggggat gatcaggatc gagatgcacc
     1981 ggagcaacga ccaggtcata ggtccctgcc tgtgcggttt ttacgtcaat ggtgctctct
     2041 ccacggtaca catcaacgca atccacctga tcaccccggg ctgcaaacgc ttcacctatc
     2101 ttttttccgc cgtggatggt gtccagtact aggatgtgca taaaaaagaa gttacaggag
     2161 tgagagcgct ttagtcgcgt tcttgatcat caggtttgca agcaggggat cgctcccgat
     2221 tggatcatca tataccagcg ggatcgatct gccattcagc aaaaatactc cctttttggt
     2281 gccttctgca agtccaatct ctccggggat gtctttctcg atgtgaacac cctttgcaag
     2341 aaacagggga acgacaacaa gggcatcaat cggttcatgc ttgaattcat tcagtgaatc
     2401 cttgattgtc ggcgtattca tattcataaa tccgcatttg acaataaaat cagtattttg
     2461 tcccttgata atagctgcgg ttttctcaac gagctcctga ttgtagggca tcgtactccc
     2521 gtgcccgaca agtaacattc ccttcttacc catataacac atagtattac cgggaatatt
     2581 aaaaattctt accaaatggt tttttgagat tatgacatcc cgaccatcaa agtcgcgggc
     2641 aatggaaagt aatttggttg atcagcctca acagatatga atctatgatg gatgctttcg
     2701 agcggtttgg actgctcatt aacgaccgga ttgtcaggac tcctgttgcg attgcatcca
     2761 tggcgggctg ggtggacgca gcatacgttc tggaacgtgc cgatcatatc ggggctgcct
     2821 ttatcggtgg gtactcaatt gaccagccca ccattgaagc tgccaggatt atctctgatg
     2881 agggcaagcg taaagaattc ctttacgatg atcctgtcga agaactcaaa aaacagatgg
     2941 ctcttttgga aaagagcagt gttgtactgg gacttaacct ccgtggcagt acacctgctt
     3001 cattcagcgc tgttgcacga tcactgggtg agaatgttgt gtacgaaatc gatgcccatt
     3061 gccgtcaacc cgcaatggta gctgccggtt gcggggaaca ttacttaaaa aaacccggcg
     3121 acctgatggc agttgtcagg gcacttaaat ctgaaggcgt taccgtatct gtaaaaatcc
     3181 gcgcaggtat atccgcagat gaccggttac ttgcccggaa actatgggaa gccggtgcag
     3241 atattctgca tattgatctt atggacttcg gttcgccgaa aatccggcag atacggaacg
     3301 tctgtccgct catgctgatt gccaataatt caatcaatac ctttgagagg atgagagaga
     3361 tgctctccca cggggcggac attgtgtcac ttgcacgcaa atctgatatc cggacattgt
     3421 ccgggctgga tgcagcaatt acccgatacg cgactgagga aggatggtac aattctccaa
     3481 aacagttatg cagaggggga gatgtccggg cacttgcatt ttgctgcatg ccggtcaaag
     3541 agtgcccact tattcctaca ctttcccgga tagggatgcc aaaagaagag tatatccgga
     3601 tgaaactgga taccgtaaaa ggcaccccac ttgaagaggg aaatcaaacc tgtttcggaa
     3661 gcctggcctg gtgttgtaaa acctcttctc cctgtatgtt ccgcgatatg acccttaaac
     3721 agaggggact gtcaaagaaa gagtatatgc gcctgaaacg cgagctctca gatacgataa
     3781 tgagccgtgt tttccatgac gtgccttctg atgagagcag ctgaacgcgc acagcttgca
     3841 atgatgctgg aagtctgcgc ttacccaaag cctgggaatg ttgaccgctg tcatgactat
     3901 ccggataccc gtcttgaaca ttttctggcg tcaaccatat ttgcacggca ggcgtttgaa
     3961 gagtccgaga ctggtaccgg gcgaattggg gagatcatca agcatgccgt ccgagatacc
     4021 aattgccatg aagggggcaa tacacatttt ggcgcatttc tcctcctcgt tcctctcatc
     4081 tacggcaagg gtattcccgg cgctttgatg gcaatagaaa aaaccgacgt atcggatgcc
     4141 gtcgcgtttt acaaagcctt tgccatgaca tctgtaagga tgaatgccgc ggatgaactg
     4201 gatgtgcatg atccccatac tctcaccatg atacgggacc gggaaatgac gcttcttgat
     4261 attatgcagc attcggcggc aaatgatatg gtagcccgcg aatgggtgac gggattccgc
     4321 cttacacggc ggggtgctga tctccttaaa aacttcggtc cgggcaggca ggcaattgtc
     4381 aatacattcc ttaccctcct tgcctctgaa ccggatacct tcatcatcaa aaagcatggc
     4441 actgaaattg cggaggaaac catgaccagt gcccgtgagg ttcttgacgg cagttataca
     4501 atagaggatt ttgatgcgga ttgtattgaa cgggatataa acccgggttc aacagctgat
     4561 atcgtcattg catcaatctt tattgcactc ggggagggat ggaattggga ttattaaaaa
     4621 gcggtataaa tgaggtgatc gcaacaaccg tgttcaatgc agctcctatg ggcattcatt
     4681 accgtgaagg aaatgcgagt atggtgatct tttcagggag taacactgca gaaaacattg
     4741 agcgtgatgg ctgggttgtt gcaaattttg tgcatgatcc ggttgtctat gtgaggacgg
     4801 catttgaaga tctgccgcga attgctttta ttgaagaacc ggttgcaaat aaaacaatga
     4861 tgaggctcgc gggtgcagat gcctgggccg catttacggc taccatcgtg cgcaaaacaa
     4921 atgagtccat gatggtccat ctgaggctgg aaaaggagat attcgaagaa gtcacgctcc
     4981 acccggtaaa ccggggcttt aatagtatca ttgacgctac cgttcacgcc acacgataca
     5041 gggacaaccg ggatccggaa ttaaaaaggc tgattgatca tcatgtgagt attgtccgca
     5101 agtgcggggg gaaacgggaa attgaagcct tggatctgtt gctgaagtat atttaaaaga
     5161 cagtgcaggt tccggttttt tttaaccgtg tcaaacagat tatcaggagg tatggtgggg
     5221 acccgggtca ttgcgagttt ttttgatgaa tttttgcttt tgtccgttaa aaagtggtag
     5281 atgcgcctga cagccggcaa agatactgag gatgattcat tccccttttt aagtatgaac
     5341 ctgctccagg aaaaaccgtg gtgcaatccg ggtcgtgtgt ggcaaaaaac ataataaacc
     5401 ctgtaatcta tttttaaaaa aaatcccgat tttaaccctg atcagcaaac acggggtacc
     5461 gggtccccga ttggcggttc aatgtatcgc tctccaccga tcagtgtttc cataataaca
     5521 tgggaacctt tcgtgacggt tccgacaaca gctgcatctt tcccatactt atgtgagtgg
     5581 agggctgcga ggacagcatc tgcacaggag ggaggaactc ccataacgac cttgccttca
     5641 tttgcaactt caagcggatc tatcccgagc attgctgccg cactcctgac actttttcgg
     5701 acaggaagcg attcttcccg gacccggata cctacaccac tctttcttgc catttcattg
     5761 atagcgcttg caaatccacc ccgcgtcggg tctttcatcg catgtattat gccggcatcc
     5821 agcacctttt gcaccattcc ccagagtggt gcgacatcag acttgatttg ttcgccgaga
     5881 tcgaatccct cccggtgtgc cattatcgct attccatgat cacccagagt cccggaaacg
     5941 ataatcacat cccctggaac aagaccattg tcacgtacaa tcttttttgc tataccgatt
     6001 ccggatgtat taatcacgat accatcgagt gctcctttct ccattacttt tgtatcgccg
     6061 gtgacaaggt ttgcacccgc ttcaccgagt gcttcgtcca tggatgccac gatcctttcc
     6121 aggttatcga tttcaaatcc ttcctcaatg atcatgccgc atgaaagtgc gattgggcgc
     6181 ccgcccatca tcgccagatc atttattgtc ccgcagacag atatcctgcc gatatcaccc
     6241 ccgggaaaga aaatggggcg gacaacgtga gaatctgtgg taaaaacgag gttctcgcca
     6301 ttcaatggga tcactgcacc gtcatccatt gcctcaagcc cgattccacc ggcattattg
     6361 tgcctgaatt tggtgagtgt ctgcaggagt tcccccatca cttctccccc tgcaccatgc
     6421 atcatgttca ccttcatgtt agtcatccac ctcgatttca accttggtga ctacaatctc
     6481 cctgcctttg accagttcgg gaagtgcgcc acattgaggg cagacaaaga tttcctcacc
     6541 ggaatatccg caggaacatt ttgtaaaggg aggcatctca ttgcattcga gtattgctgc
     6601 tgaaaaaagg ggatcatctt ccttgatggt attgaaaagg aaaatcacct gttccggatt
     6661 caccatcgcc atcttcccga cttcaacact caccttcttc acctgactgg cattgttctc
     6721 gattgcggcc ttacgggtgg ttgcgtacaa gtcatatgca atggagtact cgtgcatcaa
     6781 tcctccagtg ctgcatagac ctgtttaaag atctcccggg tctccagacc ttcttcgcgg
     6841 gacagcctct ggatagcaaa acccacatgc acaagcacaa actcgccgat cttaacatct
     6901 actaggtcga gcctgacctc ctgtctgaga tcgccataat ccacaagccc gatatttccc
     6961 tcctttattt caagcacttc agcgggaact gcaacgcaca tctgtgatcc tctggaatat
     7021 ccaacatacg ttggaggttc aaccacataa aaacaatgat aataccctta aaacaggcca
     7081 acggttcttt ttgctatcct tcaatatcag tttcacatcc tgcaatccga acattaataa
     7141 aaaaaccggt attgatataa aggtatgaat gcaaaaaaaa attgccgttt ccggtagtgt
     7201 tgcgggaatc ctcatcctca tcctcatggt tgttatcaat gtgatggtga acatggttat
     7261 tcctgcagac ataagcaggt acgggggcat gagagctgca gacgatccgg taatgatcct
     7321 gttcttcttc taccctttcg tagtggcgtt tgccgctgca atcatctttg atgttgtcaa
     7381 ggactgcctg aagggaacac cggtgcaaaa gggactgatg ttcggggcac tgcttctcgt
     7441 gatgatgacc atccccagcc tctacgtgat gatcacatcg atgacatggc caattgattt
     7501 ctacatcagc accgcaagct gggagatcat ctcgttcccc ttgatgggga tgctgtttac
     7561 caaaatctgg gaactctcat gaccgagcca ttaaatgagc attgtatcct ttcctgatac
     7621 gaaaatcctg aacaagaaat acgccgggga agagatttga actcttgagg tgcagggcac
     7681 cagtagcttt caaggctacc gccttcccgg actagactac cccggcaatg ctccatatgt
     7741 aggatgtccg atataaaaaa ggcttcttta tcagtggagt ggtcaggagt agttttttga
     7801 tcactttttt cactggtttc ttctgactat tatgaacgct gtccccaagg ccac
//
LOCUS       DASRLV010000132         6056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_288440,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000132 DASRLV010000000
VERSION     DASRLV010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288440"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..999
                     /locus_tag="VFC43_05205"
     CDS             67..999
                     /locus_tag="VFC43_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HZK30616.1"
                     /translation="MSANRYIRFVELSSGLIKDSRIPLFSSKFSKKTYTQHQLLILLL
                     LKEYLAEDYRSTVELTEIMDSLREKIHLDEVPHFTTIHKFCQRIRSSTFTRLLNRLIK
                     LFYDWGERITCTAIDSSGFTSSYASSYYSWRTEKTRKRFLKTSISVDTDRQIITGFKI
                     SQQPVHDIPHAEKLLVQCHRTRKSDTYVLDKGYDSEDIHRLIRDDLNAYSLIPVRTRK
                     RKRIYGVYRRELAKSFDQSYYHRRNLVETVFSVLKRKFGEALKARKFQAQIKELKIKL
                     ILYNISKIILAIFIRIYYWGILQSRKTLFSVVLF"
     gene            complement(980..1396)
                     /locus_tag="VFC43_05210"
     CDS             complement(980..1396)
                     /locus_tag="VFC43_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CS domain-containing protein"
                     /protein_id="HZK30617.1"
                     /translation="MQNNPNGPYDDVFNNLAKIVEDIVKNMPDNQHARIVGYTIITRH
                     ASNGDPEIFRAGEPGDDGEIPYEVVESDEMIYITATVPSPQKNAPSVDIQPESVHIGL
                     DNRDVNIMLSHPIDRIHSYYRIHHGVMDFTLKKVPH"
     gene            complement(1386..2009)
                     /locus_tag="VFC43_05215"
     CDS             complement(1386..2009)
                     /locus_tag="VFC43_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HZK30618.1"
                     /translation="MYDAVEVARLLDILGNRNRRRIIELLRQKPCFVTEISENLMLSP
                     KAVIDHLKMMEREAILSCQMDDRRRKYYYLENDILIDINLKQIQGLEIVIEERTKGEQ
                     LKKSVILLGRMIKAHDMILSNLEQVNHDIETKISDLARNHKDLFRSDREITVIIALSH
                     DSLTFQELQQVTDLSQTDLAEILKRFERKGLLGRKKDSYILRGVYAE"
     gene            2213..2440
                     /locus_tag="VFC43_05220"
     CDS             2213..2440
                     /locus_tag="VFC43_05220"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HZK30619.1"
                     /translation="MERQAVESCNLKTVGYDNNLKNLEIEFHSGMVYQYQNVPSHIYA
                     NLLSAQSTGTFFTDKIKNRYRSVRLDKVTIR"
     gene            complement(2844..3503)
                     /locus_tag="VFC43_05225"
     CDS             complement(2844..3503)
                     /locus_tag="VFC43_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HZK30620.1"
                     /translation="MDYGTMVGESFEYAKEAVVGKWNKWLMLIIATILLGLPLMGYAM
                     KVLRGEKPAPEVDDWGTLFIDGIKYLIVALIYAIPLIIVWVIVIGASAVSMMSGDYTA
                     MMAAFGAIALGLVIMFVLGVIIAVFEVIGMVRFARTGSIGEAFNFNAILATIGKLGWV
                     PYIIALVVLIVVGIVFGIIVAIIQMIPILGMIIYLCLIAPWTLFVARYVCQIYDSAGA
                     A"
     gene            complement(3643..5127)
                     /gene="purH"
                     /locus_tag="VFC43_05230"
     CDS             complement(3643..5127)
                     /gene="purH"
                     /locus_tag="VFC43_05230"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZK30621.1"
                     /translation="MKWALLSVWDKTGIIELAQELLQQKYSLMSSGGTGKVLAESGVK
                     FTEVSSYTGFPEMMDGRVKTLHPKVHGGLLGRRQIDDAVMARHGINRIDLLVVNLYPF
                     EKMSRESMSLESLIEFIDIGGPAMIRAAAKNYKNVAVVVDPDDYHIIISAIQAGDISP
                     DQRYAFAKKAFARTAAYDAAISNYLYRIDTSFPPTFTVQYTHGRTLRYGENPHQEAAV
                     YGNSGIAGAEPVQGKQMSYNNYLDVNAGVALMREFDDPAAVIIKHNNPCGVAIGTDLL
                     DAYSMAREVDPVSAYGSVVCLNREIDKRVADEIAGTFVEVLVAPSFSKESLAITAKKE
                     NMRVLLIPEKSESDEIRSIDGGVLVQRTPPYQEHWEVITDRDPTPDEMSALTLAWKVC
                     KHTKSNTIIFANKQRTLGIGAGQMSRVDSAKIAIEKACASLKGSAVASDAFLPFPDTL
                     EVAAQAGATALIQPGGSIRDKEVIEAANRFHMAMIFTGVRYFRH"
     gene            5417..5959
                     /locus_tag="VFC43_05235"
     CDS             5417..5959
                     /locus_tag="VFC43_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HZK30622.1"
                     /translation="MHDAEHSTEQNRAQDCDTAENPLPESQQSEEETEVQKTAFAELN
                     DQLLRLAADYENFKKRTARERETIISRANERFAIDILEVVDNFERALKTDDTHLREGI
                     LQIRQLLDTQLLRNGIIPLDAQKKQFNPEEHEAIAHVTSEEPAGTVIDEVSRGYRMHD
                     KVIRYAKVAVSKGNQKDQEA"
     gene            5964..>6056
                     /locus_tag="VFC43_05240"
     CDS             5964..>6056
                     /locus_tag="VFC43_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZK30623.1"
                     /translation="MAKEKVLGIDLGTTFSCMSIMEAGKPIVIPN"
BASE COUNT         1744 a         1390 c         1275 g         1647 t
ORIGIN      
        1 ccacaaaagc taaggtacgg tatctcctta ccttgttagg cgaataacca gcaaggagac
       61 gagataatgt cagcaaaccg gtatatcagg tttgttgaat tatcttccgg cttgatcaaa
      121 gattcccgaa tcccactatt ctcgtctaaa ttctcaaaga agacttatac ccagcaccag
      181 ttactcattc tccttctctt aaaagagtat ctggctgaag attaccggtc aacagtagag
      241 cttaccgaga tcatggactc tcttcgagag aaaattcatc tcgatgaagt acctcatttc
      301 accacgatcc acaagttctg tcaacggatt cgatcctcaa cattcaccag gttacttaac
      361 cggctcataa aattgttcta tgattggggt gagaggatca cttgcaccgc gatcgattcg
      421 tcgggtttta ccagttctta tgcaagttca tattattcct ggagaaccga aaaaacccga
      481 aaacgattcc tgaaaacatc aatctctgtc gatactgacc ggcagatcat taccggcttc
      541 aagatctccc agcaaccggt ccatgacatc cctcatgcgg aaaaactcct cgttcaatgt
      601 caccgaacga gaaaatctga tacctatgtc ctcgataaag gatacgattc tgaagatatt
      661 cacagactta tccgagatga cttgaatgct tattctctta tccccgtcag aaccagaaaa
      721 cgaaaacgga tctacggagt ctatcgacgt gaactcgcca aatcgttcga ccaatcgtac
      781 taccatcgaa gaaacttagt cgaaactgtt ttctctgtcc ttaaaagaaa gttcggagaa
      841 gctttgaaag cacgaaaatt ccaagcgcag atcaaagaac tcaaaatcaa attgatcctc
      901 tataacatct cgaaaattat tcttgcgatc tttatccgaa tctattactg gggaattcta
      961 cagagccgaa aaaccttatt cagtgtggta cttttttgag ggtaaaatcc ataaccccgt
     1021 gatgaatgcg gtaatagctg tggattctgt caatagggtg actgagcatg atgttgacat
     1081 ccctgttatc aagaccaata tggacgcttt caggctggat gtctacactg ggagcatttt
     1141 tttgcgggga tgggactgtt gcagtaatat aaatcatttc atctgattcc acaacttcat
     1201 acgggatttc gccgtcatca ccaggttctc ctgcccggaa gatttcagga tccccgtttg
     1261 aagcgtgccg ggtgatgatg gtatagccaa ctatgcgtgc gtgctgattg tcaggcatgt
     1321 ttttcacgat atcttcaacg atttttgcga ggttgttaaa aacatcgtcg taaggaccgt
     1381 ttggattatt ctgcatatac gcctcgaagt atataactgt cttttttcct cccgaggagt
     1441 cctttacgct caaaccgctt aagaatttct gcgagatctg tttgggacag atccgttacc
     1501 tgttgcagtt cctgaaacgt aagtgagtcg tgcgagagtg caatgattac agtaatctct
     1561 cgatcgctcc tgaataaatc cttatggttt cgggcaaggt cactaatctt ggtttcaata
     1621 tcatggttta cctgttcaag gtttgagagt atcatatcat gcgctttgat catccggccc
     1681 agcaggatga ctgatttttt taattgctct cctttggttc tctcctcaat aacaatttcc
     1741 agtccctgga tctgtttaag attgatgtcg atgagaatgt cgttttctag ataataatat
     1801 ttccggcgcc ggtcgtccat ctggcaggaa aggattgcct cccgctccat catcttcagg
     1861 tgatcgataa ccgcttttgg cgagagcatc aggttttctg atatttcggt cacaaaacag
     1921 ggtttttgcc gcaataattc aatgatccgc cttcgattcc ggttcccgag gatgtcaagc
     1981 aggcgggcta cctcaacagc atcgtacatg tttacgtaat gttagtattg ccaatattta
     2041 tgtatatcac gaaaaagggc cgtaaaaaag gaagactctg gaaatcttaa tatcgtggaa
     2101 tgccgaattg ccctcaaatt atcagacagt gttgattttt catatatctg tctcttttta
     2161 ttattaccaa gcattatctg ttgacagcac ccaatagtaa tggtgatttc tggtggaaag
     2221 acaagccgtt gaatcctgta acctgaaaac agtaggttat gataataatt taaagaatct
     2281 ggagattgag ttccattccg ggatggtcta tcaataccag aatgttcctt cacacatcta
     2341 tgccaatctg ttaagtgccc aatcaaccgg gacgttcttt actgacaaga tcaaaaacag
     2401 atatcggtcg gtacggttgg acaaagtcac catccggtga tggaaaaatt tcagtcctga
     2461 actttttttt acaaggaccg gctccctgat tactttttat attctattga aaaccgtttt
     2521 ctctgttaaa ataatacccc ttcttgcact accagttcca ttgggggact gggagtagtg
     2581 cgatgacgcc ggtttggtaa aaggtaatac atcatcatcg caatggggat gctgtggtgc
     2641 gggggcaagg accaaatgac gtcagaattt ttcagaataa tttctggaga gcacttttct
     2701 ctctctatta acccctcata accccattac taccgattga aaagaagtaa tatcccccgg
     2761 atttttcaaa aatcaattcc ctgaggaaaa aacgttctaa ggaaatccta tccggatttt
     2821 gtgaaaaaca aaaaaatatt ggattatgct gctcctgcag agtcgtatat ttgacaaaca
     2881 tagcgggcga caaataaggt ccatggagcg atgagacaga gatagataat catgccgagg
     2941 atgggaatca tctgaattat ggcgacgata attccaaaga ctatccccac gacaataaga
     3001 acgacaagtg cgatgatata agggacccag ccgagtttcc cgatggttgc aagaatagca
     3061 ttaaaattaa atgcctcacc tatactccca gttcgggcaa atctgaccat accgatgact
     3121 tcaaagacag caatgatgac gccaaggacg aacattatca caaggccaag agctatggcc
     3181 ccgaatgctg ccatcattgc cgtgtaatca cctgacatca tggaaacagc tgatgcaccg
     3241 attacgatga cccagacgat tatcaggggt attgcataga taagggcaac tatcaggtat
     3301 tttataccgt caatgaagag tgtgccccag tcatctactt ccggggcggg tttctcgccc
     3361 ctgaggacct tcatggcata ccccataagg ggaagaccga gaaggatcgt tgcaataatc
     3421 agcatcagcc atttattcca cttgccgacg acagcttcct ttgcatattc aaaggattca
     3481 cccaccatag taccataatc catacttgta aacctcttga atggtatttt ctgtcaatca
     3541 tccatgacct gtcagccata aacgatttaa ataagctatg gttatttcgc agaatataaa
     3601 tttatcgtat aaattgaccc aaaattaaaa acaagagcac aatcagtgtc tgaaataacg
     3661 tacgcccgta aatatcattg ccatatggaa acggtttgca gcctctatca cttctttgtc
     3721 ccgtattgat ccaccaggtt gtatcagagc agttgctcct gcctgagcag caacttcaag
     3781 cgtatcgggg aacggcagga acgcatctga cgccactgca gatcctttga gtgacgcaca
     3841 tgctttctca atggcaatct ttgcagaatc aaccctgctc atctgcccgg cacctatccc
     3901 cagggttcgt tgtttattgg caaagatgat tgtgttgctc ttggtatgtt tacacacttt
     3961 ccaggcaagg gtcagtgcgc tcatctcatc cggagtcggg tcccggtcag tgatcacttc
     4021 ccagtgttcc tggtaaggag gtgttcgctg gaccagcacc ccgccatcga ttgaccgtat
     4081 ctcatctgat tccgattttt ccggaattaa aagaacacgc atattttcct ttttagccgt
     4141 gatggcaaga ctctcttttg agaatgatgg agcgaccaga acttcaacaa aggttccggc
     4201 tatttcatct gcaacccttt tgtcaatttc ccggttcagg cagactacag atccatatgc
     4261 ggaaacagga tcaacttccc gtgccatact gtatgcatca agcaaatcgg tacctatcgc
     4321 aacaccacag ggattattgt gtttgattat gacagcggca ggatcatcaa actccctcat
     4381 cagggcaaca cctgcattaa cgtcgaggta attattgtat gacatctgct ttccctggac
     4441 aggttcagca cctgcaatgc cggaattacc atacacggcc gcctcctggt gtgggttttc
     4501 cccgtacctc agcgtcctgc catgggtata ctgtacggta aacgtgggag gaaaggatgt
     4561 atcaattctg tagagataat tgctgatagc cgcatcgtag gctgcagtcc gggcaaaggc
     4621 cttttttgca aaagcatagc gttgatccgg agaaatatcc cctgcctgaa tcgcagaaat
     4681 tattatgtga taatcatcag ggtcaacgac aacagcgaca tttttgtagt tttttgctgc
     4741 agcccggatc atcgccggcc cgcctatatc gatgaattcg atcaatgatt ccaggctcat
     4801 ggattccctg gacatcttct cgaacgggta caggtttacc acgagcagat caatgcggtt
     4861 aattccgtgc ctggccataa cagcatcatc gatttgtcgt ctgcccagca ggccaccatg
     4921 aacttttggg tggagggtct ttacccttcc atccatcatc tccggaaatc ccgtataact
     4981 ggaaacctca gtaaacttaa ctcctgattc ggcaagcact tttccggttc ctccggaact
     5041 catgaggctg tatttttgtt gcaaaagctc ttgtgcgagc tcgatgattc ccgtcttgtc
     5101 ccagactgag agcagtgccc acttcatggt ctagtgtatg cataatagga aagaaaaatg
     5161 tatgcaggct attcatttca gaatcgggaa tataattttt ccggcaggaa ttacctgtat
     5221 attctctgaa cagtttgggc atttcataat caaatcaact aaatcctgta taaattgcct
     5281 gaatattgaa ttgtgcgact aaatgcaccc aattatcaaa aacaggtaat attcagcggt
     5341 tcccttacag atattatgtg aaattatata tagaaccaca atacaataga taatgaaaca
     5401 tcgaacggga tgtactatgc acgacgcaga acacagtaca gaacaaaaca gagcacagga
     5461 ttgtgatacg gcagaaaacc ccctgccgga atctcagcag tctgaggaag agaccgaagt
     5521 gcaaaagaca gcttttgccg aattaaacga tcaactcctc aggcttgcag cagactatga
     5581 aaattttaaa aaacgtacag cacgtgaaag ggagaccatc atttcacgtg caaatgaacg
     5641 attcgctatt gacatcctcg aagtggtgga caactttgaa cgggcattaa aaacggatga
     5701 tacccatttg cgtgaaggca tccttcagat ccggcaactg cttgataccc agttactgcg
     5761 caatggtata attcccctgg atgcacaaaa aaaacaattc aatccggaag agcatgaagc
     5821 catcgctcat gtcacgtcag aagaaccggc gggcaccgtg atcgatgaag tatcacgcgg
     5881 gtatcgcatg catgataaag tgataagata cgcaaaagtt gccgtatcca aaggaaatca
     5941 gaaagatcag gaggcataaa aaaatggcaa aagagaaggt tttaggaata gatctgggaa
     6001 caaccttttc ctgtatgtcg atcatggagg caggaaagcc aatcgtgatc ccaaac
//
LOCUS       DASRLV010000133        11941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1102270,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000133 DASRLV010000000
VERSION     DASRLV010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 11941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11941
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102270"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VFC43_05245"
     CDS             <1..685
                     /locus_tag="VFC43_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HZK30624.1"
                     /translation="LIFLNEVRNVLRKLPHVDDSCTIVIAGYPNVGKSSFIRHVSSAD
                     PEIASYPFTTKGIIVGHRIMGRERIQFVDTPGILDRPSEQRNAIEKQALSAMMNVADI
                     ILFILDPSEHCGYPMDVQLNLLKEVKGMIEVPLVVVANKSDIVGAEGYLTMSTQTGDG
                     VDAVLAEILVHRPEPVEREKSAVLDIRSPLIYEPEETEMDEYSSTEKDKNISRKRKYR
                     KPRTTHEVI"
     gene            complement(1152..2039)
                     /locus_tag="VFC43_05250"
     CDS             complement(1152..2039)
                     /locus_tag="VFC43_05250"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042500 - aspartic endopeptidase activity,
                     intramembrane cleaving [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presenilin family intramembrane aspartyl
                     protease PSH"
                     /protein_id="HZK30625.1"
                     /translation="MDIKRILPFMVMPLLLLAVEIGSILISLPVQAAGITAFEDPTSI
                     ANPFIFIAILLVFTGFLLLLIKYDLKKVIAAIIGLSIFLTFGYIFAALVPVVLGNTDL
                     AMIAVLILSVLATSLLYIYPEWYVIDSLGILIGAGVASIFGVSLDILPVVLLLILLAV
                     YDAISVYKTKHMITLAEGVIDLKTPILFVVPKRRDYSFIRDGIGKLDDGGERAAFIIG
                     MGDLIMPSILVVSANVFLKGWRFGGIINLPALGAIIGSLAGLSVLLYFVSSGKPQAGL
                     PPLNGGTILGFLAGWSLMG"
     gene            2096..2410
                     /locus_tag="VFC43_05255"
     CDS             2096..2410
                     /locus_tag="VFC43_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30626.1"
                     /translation="MDIREYLIDILLTIVMIISTLVLINRFWQDLTIAIAATLMMLSL
                     GGLFLSLQIKMRHLERSIVIRERILRNNLEEISNRMAEKYDMAITHLDELVGEYSKRT
                     YR"
     gene            2433..3434
                     /gene="fen"
                     /locus_tag="VFC43_05260"
                     /pseudo
     CDS             2433..3434
                     /gene="fen"
                     /locus_tag="VFC43_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease-1"
     gene            3589..3738
                     /locus_tag="VFC43_05265"
     CDS             3589..3738
                     /locus_tag="VFC43_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30627.1"
                     /translation="MKICSYAVIVFLVLACCGCISPSSPGFEKNTSQFAGEVRASQSY
                     YDALV"
     gene            complement(4261..4734)
                     /locus_tag="VFC43_05270"
     CDS             complement(4261..4734)
                     /locus_tag="VFC43_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30628.1"
                     /translation="MSKIVFLVLLASFLIPAGVFAASSDCPSGGANTTGGICAVGAGL
                     LTGPGMMGPGSDTCCTGNASAGYCRPAGSCGINGKDCATSDNDSCGMMGGDEGKSESG
                     YRDMMGTTWGPVSAPGAGMYGLVAVGSILVFVLIVTWIIVGILLILTLFKKLSAK"
     gene            complement(4820..5782)
                     /locus_tag="VFC43_05275"
     CDS             complement(4820..5782)
                     /locus_tag="VFC43_05275"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HZK30629.1"
                     /translation="MQKIVRTTTSGLVMLIAVLALFSGAVAAGQTQASMGNVVTLSGY
                     SYAGDTVYLFLTGPNLPQNGVALDNINRPADNGGATRVDVDADGHWVYKWNTGASGGK
                     LDAGSYTVWVADGPADLSHLSTVTYRTISVSLSVPSLTAAISGGSGPLPAGSMNFNAI
                     PDGTSVVINNLYKGMTPLNVSGLNPGTYNVTLSHFGYAKLSMPVTVQAGSVSDVNATL
                     VALTGSVFVNTTPAGARLTLDGLTAGISPATLPNITQGNHLLNVSIEGFVTRSIPVQI
                     TADQTTTVDVVLLPYVNGVDGFLPLTVALGVLVALLFARNLSGT"
     gene            complement(5956..6495)
                     /locus_tag="VFC43_05280"
     CDS             complement(5956..6495)
                     /locus_tag="VFC43_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30630.1"
                     /translation="MKIPLHFIIALIFIILVTGCLTQFSASSSPVAATTTTLTSATPT
                     SIFPDSSGLTTLITVTGQFGKTSGTFYVPGGYWELWYTADPLITGGQDSVSASGSNSA
                     LFPYLSIQVIDTKNSDRVVETVESPGGLDKTLWAKSGQDPRPWKQKFYEGNKEYRFVI
                     TTKHLKSYTIEARIPKSSS"
     gene            6702..7115
                     /locus_tag="VFC43_05285"
     CDS             6702..7115
                     /locus_tag="VFC43_05285"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZK30631.1"
                     /translation="MAIKENILEVVGGPHVAAIATVDGTLPAVRFMVLNGFPDMTFVG
                     ATMKSTKKVAQLKKNPNVAISTWSGKEFSDPYVEMKAKGEIHEDLATKKKYWNPMFEQ
                     YFKTVDNPDYVVLVFTAAEIIYHGKNMMTEEVWKR"
     gene            complement(7358..7852)
                     /gene="sixA"
                     /locus_tag="VFC43_05290"
     CDS             complement(7358..7852)
                     /gene="sixA"
                     /locus_tag="VFC43_05290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HZK30632.1"
                     /translation="MDLFVLRHGKAGQSADGPDDRKRALTADGKDEMRKVGKWMRRKK
                     FSFEIIATSPLTRAYETADIVARTLGQKERLAVWEELVPGGDMDTICYQAAQFGEDAA
                     VLIVGHEPQLSTLISRIIAGDDSVSIILAKGGLAKIRNYAFDKRPSGDLQWLLTPRQI
                     MEMQ"
     gene            complement(8528..8929)
                     /locus_tag="VFC43_05295"
     CDS             complement(8528..8929)
                     /locus_tag="VFC43_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HZK30633.1"
                     /translation="MPPALRNNRVFNPPGKILQGMDYLYTLLKFIVGGSVIVGVTLLA
                     EQVDPRYGGMLAAAPIITTLAFLFTYAEAGRESTQQLVISAFYFAIPSLMFLLAFWFL
                     MYRFSLLLSLGGAYGIWIGAVLVINRIVTDG"
     gene            complement(9130..9729)
                     /locus_tag="VFC43_05300"
     CDS             complement(9130..9729)
                     /locus_tag="VFC43_05300"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HZK30634.1"
                     /translation="MKNGHIIFAGIGIVALLWCAGTVAALEDNSAAVQPDNFSIDIAP
                     YNGTIGPESPLYGLKVAMEDLDETFTFNDTRRVEKQVDHAQIRISEVRRELELNKTGY
                     AERALEQYRQKLNLTETSLARFRSNTTGLLHAQEMIASHQAVLADLLPRYPNSAGLAR
                     AYNNSLAVEQKFGEKTQMRFDRFAGKNNKTFFKAVKRGI"
     gene            10054..10233
                     /locus_tag="VFC43_05305"
     CDS             10054..10233
                     /locus_tag="VFC43_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30635.1"
                     /translation="MKNGTVPGSLQPCVSDPQGCRNNQPSCLVGDFPPRTGRFTAESR
                     VRPAPLFRYSVWRIG"
     gene            complement(10290..11510)
                     /locus_tag="VFC43_05310"
     CDS             complement(10290..11510)
                     /locus_tag="VFC43_05310"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HZK30636.1"
                     /translation="MKRILHGIPGIILLALVLAIPVASAADGMNADLNVSKVVSSAGP
                     NQINDVVTWAITLRNDGPANATKITLSDTISPLTGLGQVTAVAGIGEYNTTTNIWSIG
                     ELKNATTATLTLKTNFSTAGTRTNRINITGLDETDPVVDNNMAEAEVHINASGPIIND
                     QPLASKLVIRPTTLNLKSKGVFTVYVTLTGTGFRPAGHWSKTPGIDYANSSLACNGAD
                     LVRTTASDKEGRTLIAKFHRADLENVTTGDGVKVTCSGMLAVNGTTIPVEGNATIRVI
                     GKNTGENTRIDNFLSRLMKFLGLNKDDVEIIQGEDGNYTVTLSLDPDNFKNPGKVKKM
                     LNTWDNETLLVTGNKNRLFNQTCDDIKNSTKTSGDDNQIQKQNIENKPDKGNKHSFSP
                     GDKSPGKSNGKGNT"
BASE COUNT         3123 a         2896 c         2971 g         2951 t
ORIGIN      
        1 cctgatattc ttaaacgagg tccggaacgt cctgcgaaag ctcccgcatg ttgatgattc
       61 atgcacgatt gtcattgccg gataccccaa tgttggtaaa tcttcattca tccggcatgt
      121 ctcctcagca gatcccgaaa tagcatcata tccgttcact acgaaaggta tcatcgtagg
      181 acaccggatc atgggaaggg aaaggatcca atttgtggat acaccgggta ttctcgatcg
      241 tccttcagaa caaagaaacg ccattgaaaa acaggcactt tcagccatga tgaatgtcgc
      301 tgatatcata ctctttattc tggatccatc agagcactgc gggtacccga tggatgtcca
      361 gctcaacctt cttaaagaag tgaagggaat gatcgaggta ccgcttgtgg tagtggcaaa
      421 caaatcggat attgtcgggg cggaagggta tctgacaatg tcaacccaga ccggagacgg
      481 ggttgatgcg gtacttgcgg aaatactggt acacaggccg gaacccgtcg agcgggaaaa
      541 aagtgcggtt ttggatatac ggtctccact catctatgaa cccgaagaaa cagaaatgga
      601 cgaatattca tccacagaaa aagacaagaa catctcacga aaacggaaat atcggaagcc
      661 tcgtacaact catgaggtaa tctgaaattc ccggttagaa agagtctgat tcatcttttt
      721 tggctctgcg gaactgttca aaaaacctga acgctcacgg gatttcaccc gcccggctgg
      781 cccaaaatgc gatacctcca gataaggatc tacggcacta tcgtgcagtg caggggcgat
      841 ccgggggacc tgtatttacc ctccttcata ctatcactgc tctttttata ctcagataaa
      901 aggaattctg cagaacccat tttatgctat tttaagatat gcaaaaacca tggcggtact
      961 aacatatccg ccagttgatt ttagtaaagt cagttcaaaa aaatcctgtc ttgagaaaaa
     1021 tgagtttact gatggactaa cgccatgggt tcgccagact cggttgacat caccgggcgc
     1081 cctttttggg tgccggcgtt tatcacaaaa atatttgaga cggggttatt gctgtaatat
     1141 gattctttgt atcagcccat cagcgaccat ccagccagaa atccaagaat agtaccaccg
     1201 ttcaaggggg gcagtcctgc ctggggtttt cccgaagaca caaaatagag cagtacactc
     1261 aaaccggcaa gggaaccgat aatggctccc agtgcaggaa ggttgataat acctccaaac
     1321 cgccagcctt ttaaaaagac gtttgcggaa actaccagta ttgagggcat gatcagatcc
     1381 cccatcccga tgataaaggc tgcccgttcc ccgccgtcat ccagttttcc gatcccgtcg
     1441 cggataaagg aatagtcgcg ccgtttgggc acgacaaaaa gtataggggt ttttagatct
     1501 atcacccctt cggcaagtgt gatcatgtgt tttgtcttat aaacggagat tgcatcatac
     1561 accgcaagaa ggatcaggag aagtactacg ggaaggatat caagcgagac accaaatatc
     1621 gaggccactc cggcacctat taggatcccc aggctgtcta tcacatacca ttccgggtat
     1681 atatacagaa gagacgttgc caataccgat agtatgagta ccgcgatcat tgccaggtca
     1741 gtatttccga gcactaccgg cacgagggcg gcaaagatat aaccgaacgt gagaaagatt
     1801 gacagcccga taattgcggc aatcaccttt ttgaggtcat attttatcaa tagcagcaaa
     1861 aagccggtaa aaacaagcag gattgcaatg aagatgaaag gatttgcaat tgaggtgggg
     1921 tcttcaaatg cagtgattcc tgctgcctgc acagggagcg agatcaggat tgatccaatc
     1981 tccacggcga ggagcaggag tggcatcacc atgaagggta gtattcgttt aatatccaag
     2041 atgcatctcc ggtcaagtag ttagattaat tactgtgctc tcatcatcta aaagtatgga
     2101 catacgtgag taccttatcg atatcctgct caccattgtt atgataattt ccacgctcgt
     2161 actcatcaat cggttctggc aggatcttac catagcaata gcagcaactc tcatgatgct
     2221 ttcacttggc ggactcttcc tatcgctcca gataaagatg cgccacctgg aaagaagcat
     2281 tgtgatccgt gaacggatat tgcgcaacaa tctcgaggag atctccaacc gaatggcgga
     2341 aaaatacgat atggcaatta cccacctgga tgaattggtc ggggaatatt caaaacgaac
     2401 gtaccggtaa tttttttatt tagtgaatca ttatgggcgt cgcactgcgg gatataattg
     2461 cggattttaa gacaccggtg acatgggatt cacttccggg tgttgcagct atcgacgcaa
     2521 acaacgcgct ctaccagttc ctgacgatca taaggcaacc tgacgggaca cccctcatgg
     2581 accagcaggg acgtgtcacc tcacacctct ccggtatcct cttccgggtt tcgaacttta
     2641 tggagaaggg gataaagccg atctttgtct ttgacgggaa acctgctgct ctcaagcagg
     2701 agaccattaa cgggcgccgg aaaatgcggg acgaggccgg tgagaaatgg aaagaagcga
     2761 ttgagcgggg cgatgaggcc gaggcctaca aacaggcccg gtcttcatcc cgggtcgata
     2821 ccaccatcat cgggacatcc aaagaacttc tccggctcat gggcctcccg actatccagg
     2881 caccgggcga aggcgaagcc caggcggcat atatggtgga gaaaggagaa gcacggtacg
     2941 tggtgtcgca ggactacgat acgctcctct ttggcgcccc ccatcttatc cggaacttaa
     3001 ccgtcagcgg caagcggaag atccggggac gccaaatcgt tgtcaatcct gaacggatca
     3061 tccttgccga tgtgcttggc gggctgcagc tcacccgtga gcagatgatc tagatcggga
     3121 ttctcgtagg tactgatttc aacccgggcg tcgagggcat cggggcaaag acaggtctga
     3181 agatcgtgca gcgtggagaa tttgaggcca aaatccatga gaaactaccg gattttgacc
     3241 cctctccggt gatggacctc ttcctgcacc cggatgtcac ggacgactat gctttagcct
     3301 gggggcaccc tgatataacc ggcatcaaga aaatgctctg cggggaatac gatttttccg
     3361 aagaaagggt tgagaaagcc atggaaaatt tctgcgcaaa ggccgggcaa aagacactgg
     3421 aagcatggtt ttaagggatt ggcctgttca tcggggaaaa cctgagtttt ggccgtatcg
     3481 gattttttct gcccggtgta tcagtaaaac cctccatgga taaaaatcca tcatgaggac
     3541 ttcagcagag tgcatgaaga ggaaagggtt tccgactgag gaaaaaatat gaaaatctgt
     3601 tcgtatgcag tcatcgtttt cctggttctt gcatgttgcg ggtgcatctc gccaagttcc
     3661 ccgggatttg aaaaaaacac ttcgcaattt gccggtgagg tacgtgccag ccaaagttat
     3721 tatgacgccc ttgtatgaga taattcatcg aatgcaacag cgtggtgtat ccggggaaat
     3781 tactacaata atgctttcag ccagtacgat aatgcgttag cgagctacaa ccgctcactc
     3841 gaactggatc cggagtatgg gtatgcctgg ttttcaaagg gggttacctt acagaacatg
     3901 ggatatactg atgaagcgaa agaatgtttt gaaaaagcac gccggtttga tcccacgctc
     3961 ggtgcttctg ttaccagggt agagcaatga acagggatgg aacgcgggtc ccaatcctca
     4021 ggtaacccca taatttcgca accggaaacg ttctttttta aaaaaagttc aatgcacggg
     4081 aggaacatgc ttttgtttat ggggtctttt ttaaattcct gaacagatcc cgcaaccaaa
     4141 agctgcggta aaacaagaac ccggggtcgg aacgttttcc tgaaggagaa gaaatgcctt
     4201 gactgcaatg ttggaaaaaa ttcagtccgg cataaaaaaa agaaagggaa tcttttccgg
     4261 tcactttgca gataattttt taaaaagcgt gaggatgagc aggataccca cgataatcca
     4321 ggtgacgatc aggacaaaga ccaggatact tcccacggca accaagccat acatacctgc
     4381 tccaggtgcc gataccgggc cccatgtggt gcccatcatg tcgcggtaac cggattcgga
     4441 ttttccttca tctcctccca tcattccaca ggagtcattg tcactggtgg cgcaatcctt
     4501 gccgtttata ccgcatgaac cggcgggtct gcaataaccg gcgctggcat tgccggtgca
     4561 acatgtgtcc gatccgggtc ccatcatccc gggacccgtc aatagtcctg ctcccacagc
     4621 gcaaatccca ccggtggtgt ttgccccacc cgatggacaa tccgaagagg ctgcaaagac
     4681 tccagccgga atcaaaaatg atgccagaag aaccaggaat actattttcg acatggcatg
     4741 gagttcacat ttgagaatat ataggttttt attgatcact gagaatcgta atcccatatt
     4801 ctaaaatatt gaataaacat caggtccctg aaaggttcct ggcaaacaga agggccacga
     4861 ggacacctaa agctactgtt aaaggaagga atccgtccac cccgttaacg tacggcagca
     4921 gcacgacatc tacagtcgtt gtctgatcgg cagttatctg taccggaatg ctccttgtga
     4981 caaaaccctc tattgacacg ttgagcaggt gattgccctg ggtaatattc gggagggttg
     5041 ccggcgaaat gccggcagtg agaccatcga gcgtgagccg ggcgccagcc ggggtcgtgt
     5101 tgacaaaaac cgagccggtc agcgcaacaa gggtcgcgtt gacatctgat acgctcccgg
     5161 cctgcacggt aaccggcatt gataacttcg cgtatccaaa atgcgagagg gttacattat
     5221 aggtaccggg gttaaggccg ctgacgttca gcggggtcat tcccttatac aggttgttga
     5281 ttacaactga cgtcccatcc gggattgcat tgaagttcat cgagccagcc ggtagcggcc
     5341 cggaaccccc gctgatcgct gcggtgaggg aaggtaccga aaggctgacg gagatggttc
     5401 tatatgtgac cgtagagagg tgggagagat cagccgggcc atcggcaacc cagaccgtat
     5461 agctcccggc atcgagcttt cccccggagg ctccggtatt ccacttgtaa acccaatgcc
     5521 cgtctgcatc tacatctacc cgggtagcac caccattgtc cgcggggcgg ttgatgttgt
     5581 caagcgctac gccattttgc gggaggtttg ggccggtcag gaagagatag acggtgtcac
     5641 cggcatacga atatcccgaa agcgtcacga catttcccat agaggcctgt gtctgccctg
     5701 ctgcaacggc cccggagaag agagccagca cggcgataag catgactaac ccgctggttg
     5761 tggtccgaac gattttttgc attatatcct cgcaataatc ctgttgtagc cgtaccggct
     5821 cggaagatcc gggcaaaatc gggattttgg acaggtaaaa aagatctaaa gtccgatgat
     5881 ataattctac cgctggtaac atggggtcat gtaaatagca cttaatcctg ggtcaggaca
     5941 tgacgaatca attgttcatg agctactctt gggaattctg gcttcaatag tataggattt
     6001 caggtgtttt gtcgtaatca caaaacgata ttccttattt ccctcgtaga acttctgctt
     6061 ccaaggccgt ggatcctgac ctgattttgc ccacagggtc ttatcaagtc ctccggggga
     6121 ttccacggtt tctacgaccc tgtcggaatt ttttgtatca ataacctgga tcgagagata
     6181 gggaaacagg gcggagttcg atcccgaagc cgagactgaa tcctgtcccc cggtgatgag
     6241 cggatctgcc gtataccaga gctcccagta acctcccggg acataaaagg tacccgaggt
     6301 cttcccaaac tggccggtga ccgttatcaa cgtggtaagt cctgaactgt cgggaaatat
     6361 cgaggtcggg gttgcacttg ttagggttgt tgtcgtggca gcgacaggac tgctggaggc
     6421 agaaaactga gttaaacacc cggttaccag gataataaag atcagggcaa taataaaatg
     6481 cagggggatt ttcattaggt gacctgatgc ttttcaggga atatattttt ggatgggtac
     6541 ggcattaact ggtaaccgtt cattcatgat ttttaacgat agtttcctta tccggtgcca
     6601 cctttacccg ttaattcctc tcaccgtgca gctgctcatt gcatttcacc atttttatcc
     6661 ctatcgtaac tattatccga tcacaggcaa gatgaatcgc catggcaatc aaagagaaca
     6721 ttcttgaagt ggtgggaggc ccccacgttg cggccattgc cacagttgac ggtacgctgc
     6781 ctgcagtacg gtttatggtg ttgaacggat tcccggacat gacctttgtc ggggcaacca
     6841 tgaagagcac aaaaaaagta gcccagctga aaaagaatcc caatgtagct atttcgacat
     6901 ggtcaggcaa ggagttctcc gacccctacg ttgaaatgaa agcaaagggc gaaatccatg
     6961 aagatcttgc gaccaaaaag aaatactgga acccgatgtt tgaacagtat tttaaaacag
     7021 tcgataatcc tgattacgtc gtcctggtct tcaccgcagc agagatcata taccacggga
     7081 agaacatgat gacggaggaa gtctggaaga gataatccct ttcttctttt cttttccctt
     7141 cccgggaata catctcatca tcgtagtttt tcttctcttg gaaaatgaat atgaggtaac
     7201 ggtatggaaa ccaaaacaat ccaccggacc atctcctttc atgctaaaaa aggataagta
     7261 atagctggaa ttttggaata ggcagtgtct cgcgaactgc tatttttttt ttacatggaa
     7321 ataagagaga aagatgtgaa ttctgggggg atatccccta ctgcatctcc attatctgcc
     7381 ggggcgtgag cagccactgg agatcaccgg acggccgttt gtcgaatgca taattcctga
     7441 tttttgcgag gccccctttt gcgagaatta ttgagacgga atcatcaccg gcaatgatcc
     7501 tgctgatcag cgtcgataac tgtggttcgt gcccgacgat aagaaccgca gcatcctcac
     7561 cgaactgtgc agcctggtaa caaatcgtat ccatatcccc gccgggaaca agttcctccc
     7621 agacagccag ccgctctttc tggcccagcg tgcgggctac aatgtccgca gtctcatacg
     7681 cccgggtcag cgggctggtg gcaataattt cgaatgagaa tttttttcgc cgcatccact
     7741 tcccgacttt tctcatctcg tcctttccat cagccgtgag tgcccgtttc ctgtcgtcgg
     7801 gcccatcagc agactgaccc gcctttccat gccggagaac aaagaggtcc atacaagaaa
     7861 gactgtctta tggaatatga ggcttgttat tgcggggatt gcaaagtaga atgcctgggg
     7921 ggacgatcta ccggatcgcc tgccgtcctg cttcggacca ggtgaagagg aacggttttt
     7981 tcaattccgg tctcatttcg gaagtcccat gggagttaaa gaatctgcta aaagatgaaa
     8041 cacgtaaccg aggaaaaaac acgatgcaag ataaatgaat gaaagatttg gaataggtga
     8101 taagatcaac gcaattaagg ccgatagtga aaatagcagg atcatgagat ttaaagtccc
     8161 cttaccatga cagaatcccc gatgcctcca tcctgaggct ggttccaata tatcaggcac
     8221 ggctgatcct aaagctgcaa caaaaaggcc agataaccag ggataatttg ttgttttatt
     8281 gatgatttcg atgaaatact ggtaaaacaa acgcaatgcc accaattgca agatgccatc
     8341 ctcgtctcat catatttgtt gtcgtgaaga tagtgatata acctttaatg ttgggaaggt
     8401 gaaagtgaac ccccctcttc cggagatgaa ctgatctcct gaatggcgga gatccttaca
     8461 ctcctcagaa attgagtgat cctttttttt aaaaaaaata aatttcagaa ttttgaggat
     8521 ttccgcatca cccatcggta acgatccggt ttattacaag gaccgccccg atccagatcc
     8581 cgtacgcccc gccaaggctc aggaggagac tgaacctgta catcaggaac cagaaagcca
     8641 gcaggaacat gagggagggg attgcaaagt agaatgccga gatgaccagc tgctgggtcg
     8701 actcccgtcc tgcctcggca tacgtgaaga ggaacgcaag ggtcgtgatg attggcgctg
     8761 ccgcaagcat gccgccatac cggggatcta cctgctcggc aagcagggtc accccgacaa
     8821 tgacacttcc gcctacgatg aatttcagga gcgtgtagag ataatccatc ccttggagta
     8881 ttttcccggg gggattaaat acccggttgt tccggagggc agggggcata cctccccact
     8941 tcccatgttg ggggaggctc aaacagggga aaaaaattca gggtatttcc atcatttccg
     9001 ctttggtgca aagtggttac cggtttcatc agccgtagtt ttatctttta tccggtcccc
     9061 ggtctcattg ccggcctgtg ctagggtccc ggaaggtgca ggggtatccc cgccagcagg
     9121 gttctggtcc tagattcccc gtttcactgc tttgaaaaac gtcttattat tctttcccgc
     9181 gaaccgatca aacctcatct gggttttctc cccaaacttc tgttcgactg ccaggctgtt
     9241 gttgtaggca cgggcaagac ctgcactgtt cgggtaccgg ggtaagagat cggcaaggac
     9301 tgcctggtgc gaagctatca tttcctgtgc atgcaaaagt ccggtcgtgt tggacctgaa
     9361 tcgtgccaga gacgtttctg tcaggttcag tttctgccgg tactgttcca gcgcccgctc
     9421 tgcgtatccg gtcttattga gttccagttc cctgcgcact tccgaaatcc ggatttgtgc
     9481 atggtcaacc tgtttttcca cccgccgggt atcattgaac gtgaaggttt cgtcaaggtc
     9541 ttccatggcg accttcagcc cgtacagggg actttcagga ccgatggtgc cattgtaagg
     9601 tgcaatatca attgaaaagt tgtcaggctg gactgctgct gaattgtcct caagtgcagc
     9661 gactgtcccg gcacaccaca gtagtgcaac aatacctatt cctgcaaaaa tgatgtgccc
     9721 gtttttcata caacctcttt taccagtata tgttgttgaa tacaggtatc ttttttagac
     9781 aataaggatg gctattgttc ggtaaaaatc cgaacgtctg gtctccgctc aaatgcctat
     9841 taactgttca ggatagtcca gaataaaaaa taaagcggga tactcagaga ttgcctgccg
     9901 gagaactgcc ggctgcccgg aacctccggt acgcatcaca gtaccctgaa cgcgcaaagg
     9961 aagggcacag tcccaaggct gaaaacgatt atcccgggtt tggatgagga taggaccagc
    10021 cgatccgggc actcctgtcc ggcgggatac cggatgaaaa acggtacagt acccggttct
    10081 ttgcagcctt gcgtatcaga ccctcagggg tgcaggaata accagccatc ctgccttgtc
    10141 ggggacttcc ccccaagaac cggcaggttc acggctgaat caagggtacg cccggctcct
    10201 cttttccggt attctgtctg gaggatcgga tgacgggtaa tgatggatga gaaacgatta
    10261 attaaaaaaa agtggtgtgt tgcctgagat cacgtattcc cttttccgtt tgacttcccc
    10321 ggggacttat caccaggact gaacgaatgt ttattgccct tgtcaggttt attttcgata
    10381 ttttgcttct ggatttggtt atcatcgccg ctggtttttg tgctattctt tatgtcatcg
    10441 caggtctggt taaaaagccg gtttttgttg ccggttacca gtaaggtttc attatcccaa
    10501 gtgttaagca tcttttttac cttgccggga tttttgaaat tgtcaggatc cagcgataac
    10561 gtaaccgtat aattcccatc ttcgccctgg atgatttcca cgtcatcttt attcaggcca
    10621 aggaatttca tgagccggga aaggaaatta tcaatccttg tattttcccc ggtatttttc
    10681 ccgattaccc tgatagtggc attgccttca accgggatcg tcgtgccatt cacggcaagc
    10741 attcccgaac aggtaacctt cacaccatcg ccggtagtaa cgttctcgag gtctgcccgg
    10801 tggaactttg caatgagggt cctgccttcc ttatcggagg ctgttgtcct gaccaggtca
    10861 gcgccattgc aggcaaggga actgttggca taatcgatac cgggggtttt actccagtga
    10921 ccggctggtc tgaagccggt gccagtcaga gtaacatata cagtaaatac ccctttgctt
    10981 ttaagattca gggtagttgg cctgataaca agttttgaag ccagcggttg atcgttaatg
    11041 atgggacctg atgcgttgat gtgcacttct gcttctgcca tattattgtc cactaccgga
    11101 tccgtctcat ccagtccggt gatattgatc ctgttcgtcc tggtccctgc agtgctgaag
    11161 tttgtcttca gggtaagcgt ggcagtcgtg gcatttttca gctcgccaat actccagata
    11221 ttcgtagtag tattgtactc tccaatgccc gcgacagcag ttacctgtcc cagcccagtg
    11281 agcggtgaaa tggtatcgct cagcgtaatt ttggtggcat tcgcgggacc gtcattccgg
    11341 agcgtaatag cccacgtcac tacatcattg atctggttcg gtccggctga actcactacc
    11401 ttagaaacat taaggtcggc attcattcca tctgcagcac tggcgacagg tatcgccaaa
    11461 accagcgcaa gcaggattat tcctggtata ccgtgaagga ttcttttcat aaaaatttca
    11521 tcctcaatcc gggacatccc ggtataattt ttcttgaccc ggccagagtt aaggggtagg
    11581 aaagtttaac aaaaactgaa cagtcctcaa tactcctgaa catgaagaca tcgttcctga
    11641 tcgttcagaa tcaaaaataa ccagagaaaa tcctataaaa aatccggagc aaaatgcggg
    11701 ggaacagatg gagccggtcc gtttttttcc ggaagggcgc acaaaaagag tgggcacccg
    11761 gagctcctgg atgaatggaa agattgggag cgcttgaaaa aagaatcaat tattttatag
    11821 cagaggaaca agtgcctggt atcgtttggc aatccccgat cgtcatccct tctcccgcaa
    11881 aactacccct tttttacacc accgtcagtc ggaaaaaata gccccatatt ggtgttctgg
    11941 c
//
LOCUS       DASRLV010000134        16733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_857412,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000134 DASRLV010000000
VERSION     DASRLV010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 16733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16733
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857412"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..171
                     /gene="rpsQ"
                     /locus_tag="VFC43_05315"
     CDS             <1..171
                     /gene="rpsQ"
                     /locus_tag="VFC43_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HZK30637.1"
                     /translation="YLHYIRKYKRYEKRSSKLHAHSTPCLHAKVGDMVKIAECRPLSK
                     STTFVVIEVLQP"
     gene            168..566
                     /locus_tag="VFC43_05320"
     CDS             168..566
                     /locus_tag="VFC43_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HZK30638.1"
                     /translation="MKAKQSTIPRALATGTRLACADNTGARTVQIISVFGYHGVRRRQ
                     PKLGLGDLATVSVQKGTPDMRRKLVRAVVIRQKKEMRRPNGMRISFDDNAVVVVDEKN
                     EPKGTEIKGPVAREVAERFPKLGSMATIIV"
     gene            577..942
                     /gene="rplX"
                     /locus_tag="VFC43_05325"
     CDS             577..942
                     /gene="rplX"
                     /locus_tag="VFC43_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HZK30639.1"
                     /translation="MVRIESEQPRKQRKARYQAPSHVKSKYLSAPLSAALKEKYKKKT
                     LRVIKGDTVKVTRGDFVGNEGLVDAVDTKKSKIVVHGVSSTKADGTEVPRVIDASNVQ
                     LTKLNLSDKRREAKLAEAE"
     gene            942..1667
                     /locus_tag="VFC43_05330"
     CDS             942..1667
                     /locus_tag="VFC43_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4e"
                     /protein_id="HZK30640.1"
                     /translation="MSDHLKRLNAPDSWHIAKKTTKFITKTAPGPHNANAMPIAVWLR
                     DNMGLARNLKEVKQILNQNDVIINGRSCRDAKMGIGIFDIISLPKINKFYRILRDKNG
                     RHVSIEIDAEAAKTRLCKVKNKTVVAGGKVQLNMRDGANLLADNTYKSGDSIVLSLES
                     ETRFKIVDHFPFAVGNMAMVIGGRHSGKVARIVDIIKMPGSVPNKIILEDESAKTRFD
                     TISPYIYMVGKQTPAIASWGIEQ"
     gene            1673..2170
                     /locus_tag="VFC43_05335"
     CDS             1673..2170
                     /locus_tag="VFC43_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HZK30641.1"
                     /translation="MRDIYIDKVVVHMGVGESGEKLVKAENIMKTITKQTPIRSIAKM
                     TQPAFSIRKGAPIGCKVTLRGKSARDFFATSIGIINKTVFESQFDKSGNISFGIEEHT
                     DFPGMSYDPQIGIFGMDVNVVLERKGIRITRRRMEQKKLPAKQRVKKEDAIAFLKEQY
                     QVEVR"
     gene            2170..2325
                     /locus_tag="VFC43_05340"
     CDS             2170..2325
                     /locus_tag="VFC43_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HZK30642.1"
                     /translation="MAGEQKKIYGRGANECKMCGRKQGLVRRYHIMFCRQCFREWARK
                     IGFKKMS"
     gene            2339..2731
                     /locus_tag="VFC43_05345"
     CDS             2339..2731
                     /locus_tag="VFC43_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HZK30643.1"
                     /translation="MTRSNPIADGMCSLKNAGDTGKSVCIIEPASKLLGAMLRIMQDA
                     GYISSFEFIDDGRGGQLKVHLTGKINRCGAITPRFSVQMDEMEYWEKQYLPGKNFGLL
                     ILSTSQGVISHDQARKLGIGGELLGYVY"
     gene            2743..3267
                     /locus_tag="VFC43_05350"
     CDS             2743..3267
                     /locus_tag="VFC43_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HZK30644.1"
                     /translation="MSAVRTVKIPEGVKAHLDGSQLRITGPKGQLSRNVRFPQVAVTC
                     TGNEVSISTESKRKEMTAMVGTLEAHTKNMCRGVTEGFEYHMKMVYSHFPIQLKLQGN
                     RLEIANFLGEKKARYARIEPGVTAKIANDEVVLTGIDRELVGTSAANIEHATHIRNRD
                     PRVFQDGIYIVQRG"
     gene            3274..4020
                     /locus_tag="VFC43_05355"
     CDS             3274..4020
                     /locus_tag="VFC43_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32e"
                     /protein_id="HZK30645.1"
                     /translation="MTTEIKRLIRVRVEKGATFKRDGYGKKAQLSDSWRKPRGQHNKQ
                     REQKKAKGALPKPGFGSPVAVRGMHPSGFFEILVTSLKDLEGLDPKTHAVRIGGTVGD
                     RKRMILQETALASGIKVLNTRSAGIKETKPKKIDVKRFSADKKKKDEKAATKSKTSEA
                     PVKKDTKKAKDMPKSDKKKTPAKPTKKPAKPKSEKAEKTPEKTGITTPAKPAKKAAKP
                     KTETPVEAKPKAAAKPKAKKKTGSEVKKHE"
     gene            4013..4465
                     /locus_tag="VFC43_05360"
     CDS             4013..4465
                     /locus_tag="VFC43_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19e"
                     /protein_id="HZK30646.1"
                     /translation="MSDVASQKRIAAAVLKCGVNRVWFDPARISDIENAISREDLRGL
                     ITDGAIKAHQKKGVSRGRARARIAKRSYGHCKGAGKRKGAAGARNPSKTAWVQKIRAI
                     RKVLVELRDAGTIDSHMYRIIYRKAAGGQFRSVAHMKAQMEILAGRMK"
     gene            4467..4991
                     /locus_tag="VFC43_05365"
     CDS             4467..4991
                     /locus_tag="VFC43_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HZK30647.1"
                     /translation="MATGSRYFVPFRRRREGKTDYYKRTRLVVADAPRMVVRKTNRHI
                     IIQLVTAELDGDRTLVSANSAELETYGYKGSTSNTPAAYLTGMLFAVKAKKADYSRAI
                     LDIGLNRATPGARVFAALKGAVEAGLDVPHGEKILPSDERAKGNHIAAHNKNAADIVS
                     IVEQAADAIKKELV"
     gene            4992..5609
                     /locus_tag="VFC43_05370"
     CDS             4992..5609
                     /locus_tag="VFC43_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HZK30648.1"
                     /translation="MAYEREEWRPVTGLGKLVASGEIKSIDQVLESGRPIKEPQIVDM
                     FLPDLEDEVLDIAMMQRMTDSGRRVQFRAVVIVGNRNGYIGFGQGKDVQVGNAIKKAI
                     VKAKMNIIKVRRGCGSWECGCDITHSIPMQVEGTAGSVRVTLKPAPQGIGLVTGDISK
                     KVLELAGIKDVWTFARGQTRTTINFAKATFNALNETNMIRTGRSL"
     gene            5610..6071
                     /locus_tag="VFC43_05375"
     CDS             5610..6071
                     /locus_tag="VFC43_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HZK30649.1"
                     /translation="MYAVVQVRGVVKTRRDIKDTLKMLRLHHINHCVLVADTPENLGM
                     IRKVKDYVAYGEADAPTIETLLLTRGRVIGDAPLTDEYIKSNSTYSSISEFAQALASG
                     ETKLREIPGLKPVLRLHPPRKGYKTTKRTFPQGGALGYYGQEINTLLYKMR"
     gene            6078..6506
                     /locus_tag="VFC43_05380"
     CDS             6078..6506
                     /locus_tag="VFC43_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uL15 family ribosomal protein"
                     /protein_id="HZK30650.1"
                     /translation="MPTNKRSKYRGSRTCGGGTHKNRRGAGNRGGRGRAGINAHHFVK
                     WYKEMGGPVFGKDGFSNSSQITVTVMDVGIIDQIIPSLLAQGIAKNEGDAIVINTADM
                     GINKVLGSGKVTKKLNISAQAFSESAKIKIEKMGGKTQIV"
     gene            6533..7966
                     /gene="secY"
                     /locus_tag="VFC43_05385"
     CDS             6533..7966
                     /gene="secY"
                     /locus_tag="VFC43_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HZK30651.1"
                     /translation="MGNLLDRMEPLLAAMPAVKSPEGHVHFKNKLIWTLGILILYFAL
                     TNIPVFGLAPSNQDLFAAWRALLAGASGSIVHLGIGPIVTASIVLQLLKGADILHIDT
                     SETRGQVMYMGLQKILIMVMIVIEAAPNLVGSFMIPDPVIANQFFGGNLFAVSLIIFI
                     QICIGGVLIFFMDEVVTKWGIGSGVGLFIIAGISQAIVNGFINWAPVADQYPVGFFPR
                     LIAIGLDGGSYVTYFGKDILAFVTTIAIFLIIVYVESTRIEIPLAHAQVRGARARFPV
                     KLIYASVLPMILVRVLQANIQMVGMFLSNVNITIFGAFEGSQPINGIMYYLAPINGPS
                     DWMWWITDLGHAPWQVLLRLGVDITFMVVGGAIFALFWIKTAGLDSKDVARQIQLSGM
                     SIPGYRRNPQVLEKYLDRYIPRVTVIGGVFIGILSVIANLFGVIGAVIGTGLLLTVSI
                     TYRLYEEIASQQIMEMYPFMRTFFGKE"
     gene            7986..8558
                     /locus_tag="VFC43_05390"
     CDS             7986..8558
                     /locus_tag="VFC43_05390"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HZK30652.1"
                     /translation="MEGKKVIITGVPGVGKTTVVNEALKKISEEGVEYQSINFGTFMF
                     EVAKNDNIVQDRDQMRTLDRAVQKQLQQRAAQAISRIPGNVLIDTHASVKTPKGYLAG
                     LPEWVLREIMPDCIVLVETDDDQILMRRMTDETRSRDKEGSRSIAEHQQFNRSIAAAY
                     AMLTGCTVKIVINADFLLERAADDMAAVLR"
     gene            8563..9174
                     /locus_tag="VFC43_05395"
     CDS             8563..9174
                     /locus_tag="VFC43_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EMC3/TMCO1 family protein"
                     /protein_id="HZK30653.1"
                     /translation="MDFGKIWDKYGIYIALMFMMLMMFSYSIEWLRVGVGQGIDVVFA
                     PLLDWFKIPFYILIVILSALTGLYSSIIQKYTIDYEKMTGAQERMKEFQTEYREAQLS
                     QDEKKIKKLEAKKDRIMKEQLEMSQNQFKPMAYILVLTVPIFFWLLFRLGQVKSTITL
                     PYAGTIGLHDPALWIIPAWILWYMICSITISQVIRKSLNIGGI"
     gene            9174..9716
                     /locus_tag="VFC43_05400"
     CDS             9174..9716
                     /locus_tag="VFC43_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZK30654.1"
                     /translation="MRITVSGLPGSGTTSLSRYLSERHGFTMISAGEVFRDLAKEHAM
                     GLAEFGRLAQENPAFDKMIDARQKEIAEQRDNILVEGRLSGWMVPGANLKIWLFAPIG
                     CRIKRIVFRDQIEDEKTAKRITIEREECEAGRYHSYYNIDINDLSIYHIILNSEHWSV
                     EGLGSIVDTAIAQLNPSDKR"
     gene            9811..10242
                     /locus_tag="VFC43_05405"
     CDS             9811..10242
                     /locus_tag="VFC43_05405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZK30655.1"
                     /translation="MITVEKQITIQLVHSWDEYEIAELYRAGGWWKEEYNESDLRHLM
                     RGSYLFAVAVDTKTGRAVGMGRIISDGFSDGYIQDLVVLPEYRKTGIGGRIVAALVNK
                     CVEQGISWIGLIAEPTTEKFYLPYGFQRMEGHTPLLFRRDL"
     gene            10242..11135
                     /locus_tag="VFC43_05410"
     CDS             10242..11135
                     /locus_tag="VFC43_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HZK30656.1"
                     /translation="MLTQENFKPVTLEDRAFFERHYAQYPQTHSDNTFTNMVCWNHYA
                     HYKYAYVEKNIIIASTIDDITRFRPPIGPRDPALLKSLIRLASDVSDNEPIVLIDPET
                     ASWMRDICPGINLVPDRNHFEYVYRSADLADLPGKNYLTIRHQINKFRRNCSSSVEPI
                     TKENWEEVKRFLIEWCEWKGCENDKVLAHEKEAVFFAIDHFDALSMKGLIIRVFSKIG
                     AMSLFEPLNADTALVHFEKGLPDCEGIYKVINAETAAFLIKKYSYLNRESDLGIAGLR
                     EAKMRYHPHHMVEVYSLKRDS"
     gene            complement(11544..12140)
                     /locus_tag="VFC43_05415"
     CDS             complement(11544..12140)
                     /locus_tag="VFC43_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HZK30657.1"
                     /translation="MQDLLSGIIYSFAALFIILDPLLSVPIFAAMTKGQTPAEIHKQA
                     FIAVAVAGGLMYLFLIFNRMIFDILGLNMPSFQIAGGILLFLLGIQEALGIEFGHYKE
                     HAKTAAGVVIGTPLLCGPGTITTVMLLSRDYGVLIPFVAITFSLIATWLVLYYAENIQ
                     RVLGEVVTDIMGKVLGMLVAAIAVKIIASGILGYLALV"
     gene            complement(12219..13379)
                     /locus_tag="VFC43_05420"
     CDS             complement(12219..13379)
                     /locus_tag="VFC43_05420"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZK30658.1"
                     /translation="MSEAGSYKRSLGLFELVSLGVGGTIGSGIFVVPGVAAKLTGSWS
                     LLAWVIVALSASCVLLSLAYVSSQFTSSNSFYILFKSVFGARFAVPLIILYLISSVFG
                     VSTIAAGIGQYITWFGVSNVLLIEIVIIGAFCWLNITGISFSGMTENILTTLKIIPLV
                     MIAFLLIPFINVGNFSPTTPLTVTGLLATIIIVYWPFTGFEISAIPVEETRDPALIRR
                     SLIIVMGIVVTLYLLLNVSLIGSVGSEALAASPAPVATAAGILLTQSGTLIACIGIVA
                     MLSAINAYIVGTSRVIQSMSSEFFIPVLRNLNSKGTPAPALITGCAVSGGLLLFSNHF
                     DQLATISVITTLIPYIFFCLASWVLVKDTKARLISASGAISTSLILIVYFIA"
     gene            13506..14018
                     /locus_tag="VFC43_05425"
     CDS             13506..14018
                     /locus_tag="VFC43_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HZK30659.1"
                     /translation="MAKTVEKAKKSMGIKAKKPAAAKTKAAIKKPAAKGKKGGPSKKE
                     GTQMRYKCRICGYVYSPLRGEPHNGIPAGTPFEDLPETYLCPVCGFQGKGKVGKWGFD
                     EWRPTRYICSMCDYIYDEKRGEPHRGIKPGTKFEDLPEDYVCPVCATDPKIVLRYGNV
                     LKQGFAPLYE"
     gene            14274..14879
                     /locus_tag="VFC43_05430"
     CDS             14274..14879
                     /locus_tag="VFC43_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZK30660.1"
                     /translation="MEVHHQLAEKAHHFMRIGRYDEAIDLYKKLLESDRKNTTYLENI
                     GKMYYSLGRHEDAIAWYDKAVAINPKLPSVWFEKGYALRKIHRYEEAIMCFDQVLTLD
                     PDNALARANKGYALNESGKFREALESFDTLLKTSPKNLRVMTAKGISLSKLGNNTEAL
                     EYFDKVISYNPLDYYVQNQRAAVLRNLGRIQEADEFVKKGK"
     gene            complement(15005..16042)
                     /gene="mtrH"
                     /locus_tag="VFC43_05435"
     CDS             complement(15005..16042)
                     /gene="mtrH"
                     /locus_tag="VFC43_05435"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit H"
                     /protein_id="HZK30661.1"
                     /translation="MFKFEKEQSVWDFNGTMIGGQPGEYPTVLGASIFYNKHEVVLDD
                     KTGKIDKAKAEALWNRCQVLSDLTGIPHFIQIIAEYGEAFESYISWFDSIDNKTAFLM
                     DSSVPKALAHACKYVTDTGLAHRAIYNSINGSIMPESIEALKNSDVDAAIVLAFNPAD
                     PSVVGREKVLVEGGVAGQTKGMLEIAEYCGIKRPILDTAATPLGLGSGGSYRDILACK
                     AIHGYPTGGAYHNMTVSWTWLKRWKGTSKTPSVLAAGYEGKDVLLKQMSHHYLGGMEG
                     IKQAAWSAPDIGCNMIASTLGADLIMYGPIENVEAMITAQAYTDIVVLEATRQLGIEC
                     KAENHPIFKLI"
     gene            complement(16059..>16733)
                     /gene="mtrG"
                     /locus_tag="VFC43_05440"
     CDS             complement(16059..>16733)
                     /gene="mtrG"
                     /locus_tag="VFC43_05440"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019386 - methanogenesis, from carbon
                     dioxide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit G"
                     /protein_id="HZK30662.1"
                     /translation="DANSPVAVVTMGSHLDEKGICDAGAALCGSCKTENLGLEKVIAN
                     VIANPNIRFMLFCGTEVKGHLSGQTFGALHKGGVKEGRVVGAEGAIPFIENLNDAAIK
                     RFQEQTEIVNIMESEDLNAIKAKINELKARDPGAFAADAIVVEVKEAAGGVETAAAGA
                     NPQFLEIEKRLDKIEKKIEFVDAEIAQRVGRKIGRDIGILYGLVAGLTVFMMLLFLYQ
                     KLMTLV"
BASE COUNT         4832 a         3704 c         4187 g         4010 t
ORIGIN      
        1 tacctgcact acatccgaaa atacaagcgt tatgagaaac gcagttcgaa gcttcatgct
       61 catagcacac cctgccttca tgcaaaagtt ggcgacatgg ttaagatcgc tgaatgcaga
      121 ccgctttcaa agagcacaac atttgttgtt attgaggtgt tacagccatg aaggcgaagc
      181 agtctacgat tccgcgagca cttgcaaccg ggaccaggct cgcatgcgct gataataccg
      241 gcgctaggac tgtacagatc atatccgtct ttggctacca cggcgtcagg cggcgtcagc
      301 ccaaactcgg gcttggggat cttgctacgg tgagtgtcca gaaaggcact cctgatatgc
      361 gccgcaagct ggttcgtgca gtagtcatcc gccagaaaaa ggagatgcgc agacccaatg
      421 gaatgcggat atcctttgat gacaatgcag ttgttgttgt ggatgagaaa aatgagccaa
      481 aagggacgga aatcaagggt cctgtagccc gtgaagtggc ggaaaggttc ccaaagctgg
      541 ggtccatggc aacgatcatt gtgtgaggtt tgaagcatgg tacgaatcga aagtgaacag
      601 ccaagaaagc agagaaaggc ccgctatcag gccccttcac acgtgaagag caaatacctc
      661 agcgccccgt tatccgctgc actcaaagaa aaatacaaga aaaagacgct gcgggttata
      721 aaaggggata cggttaaggt aacccgcgga gattttgtcg gaaatgaagg actggtggat
      781 gcagttgata ccaagaaatc aaaaattgtt gtccatggtg tgtcttccac aaaagcagat
      841 ggaaccgaag taccccgggt tattgatgct tcaaatgtgc aacttaccaa actgaatctc
      901 tctgataaac gccgtgaggc aaaattagcg gaggctgaat aatgtcagac catctgaagc
      961 gattaaacgc cccggactcg tggcacattg caaagaagac aacaaaattc atcacgaaaa
     1021 cagcacccgg accccataat gcaaatgcga tgccaattgc agtctggctc cgcgataata
     1081 tgggtctcgc acggaattta aaagaagtaa agcagatcct caatcagaat gatgttatta
     1141 tcaatggaag gtcttgcagg gacgcaaaga tggggattgg tatttttgat atcatctcgt
     1201 taccgaaaat caataaattt taccgcatcc tccgtgacaa gaacggtcgc catgtatcta
     1261 tcgaaattga tgccgaagcg gcaaagaccc gcttgtgcaa agtaaaaaac aagacagttg
     1321 ttgccggagg taaagtgcag ctcaatatgc gggatggagc aaacctgctg gctgataata
     1381 cctacaaatc aggtgattcg attgttttgt cccttgaatc agaaacacgg ttcaaaattg
     1441 ttgaccactt cccgtttgcc gtcggtaaca tggcaatggt aattggaggt cgccactcag
     1501 gaaaggtcgc acggatcgtt gatatcatca agatgccggg cagcgtcccg aataagatca
     1561 tccttgagga cgaatctgca aagacacggt ttgacacaat ctctccctat atctacatgg
     1621 tcggcaagca gacacccgca attgcatcct gggggattga acagtgactt ccatgcgcga
     1681 tatctatatc gacaaggtag tcgttcacat gggtgtcggc gagagcgggg aaaaactcgt
     1741 aaaagctgaa aacatcatga agacgatcac caaacagacg ccaatcagga gcattgccaa
     1801 aatgactcag ccggcattct ccatacggaa gggcgcccca atcggatgca aggttacact
     1861 ccggggtaaa tcggcccgcg attttttcgc gacatcgatt gggattatca acaaaacggt
     1921 ctttgagagc cagttcgaca agagtggaaa tatctcattt ggaattgaag aacacactga
     1981 ttttcccggc atgtcctatg atccacaaat tggtatcttt ggcatggatg tcaatgttgt
     2041 tctcgaacgc aagggcatac ggatcacccg ccgacgcatg gagcagaaga aactcccggc
     2101 aaaacagcgc gtgaaaaaag aggatgcgat tgcatttctt aaagagcagt atcaggttga
     2161 ggtgcgctaa tggctggcga acaaaagaag atctatggtc gcggtgcgaa cgaatgcaaa
     2221 atgtgcgggc gtaaacaggg gcttgtgcgc agatatcata tcatgttctg caggcagtgc
     2281 ttccgtgaat gggcccggaa aatcggattt aaaaagatga gctgagggtg aatgcactat
     2341 gacacgaagt aatccaatag cagacggaat gtgttcctta aaaaatgccg gagacaccgg
     2401 taagtccgtg tgcatcatcg aaccagcgag caaacttctc ggcgcgatgc tccgtattat
     2461 gcaggacgcc ggttacatca gcagtttcga gttcatcgat gatggcagag gcggacagct
     2521 caaggttcac cttacgggta aaattaaccg ctgtggtgca ataaccccgc gcttttcggt
     2581 acagatggat gagatggaat actgggagaa gcagtacctg cctggaaaga atttcgggct
     2641 cctgatcctt tctacctcgc aaggtgtaat atcacacgat caggcgagga aattaggaat
     2701 cggtggcgaa ctgctcgggt atgtatattg aggtgcgaga tcatgtcagc agtcagaaca
     2761 gttaagattc cagaaggggt aaaagcccat ctggatggct cgcagctacg tattacgggg
     2821 ccaaaagggc agctttcacg aaacgtacgg tttccccagg tagcagtaac ctgcacagga
     2881 aatgaagttt ctatctctac cgagtccaaa aggaaagaga tgacagcgat ggttgggacc
     2941 ctggaggcac ataccaaaaa catgtgccgg ggtgtcactg aagggtttga gtaccatatg
     3001 aaaatggtct acagtcactt cccgattcag ctgaaacttc agggaaaccg gcttgagatc
     3061 gctaatttcc tcggtgaaaa aaaggcgcgg tatgcacgga ttgaacctgg tgtaacagca
     3121 aagattgcaa atgatgaagt tgtgttaaca ggaatcgacc gcgaactggt cggaacctcg
     3181 gcagcaaata ttgagcatgc cacgcatatc cgcaaccgtg acccgagggt cttccaggat
     3241 ggtatatata ttgtacagag gggctgaaga tcgatgacca cagaaataaa acggttaatc
     3301 cgtgtacgtg ttgagaaggg tgcaaccttc aaacgtgacg ggtacggtaa aaaggctcaa
     3361 ctctctgatt cctggagaaa accccgcgga cagcacaata agcagagaga gcagaagaag
     3421 gcgaaaggag ctctccccaa gcccggattt ggtagccctg tcgcggtgcg cgggatgcac
     3481 cccagcggat tcttcgaaat actggtaacc tctttaaaag atctcgaagg gctcgatcca
     3541 aagacccatg cagttcgcat tggggggacc gtcggagacc gtaagcgcat gatccttcag
     3601 gaaacggcac ttgcttcggg aattaaggta ttgaacaccc gctccgccgg aataaaagag
     3661 acgaaaccga aaaagatcga tgtaaaacgg ttttcagcgg acaagaagaa aaaagatgaa
     3721 aaggccgcca caaaatccaa aacatctgaa gctccggtaa agaaagatac gaaaaaggca
     3781 aaggatatgc caaagtcaga taagaagaag actccggcaa aaccgacaaa aaagcctgca
     3841 aaaccaaaat cagaaaaagc agaaaaaact ccggaaaaga ccgggataac gactccggca
     3901 aaaccagcga aaaaagcagc taaaccaaag acggagacac ctgttgaggc aaaacccaag
     3961 gcagctgcaa aaccaaaggc aaaaaagaag accggttcag aggtgaagaa gcatgagtga
     4021 tgtcgccagc cagaaacgaa ttgctgctgc agtactgaag tgcggtgtaa accgtgtctg
     4081 gtttgacccg gcccgtatct cggatattga aaatgcaatc tcccgtgaag acttaagggg
     4141 ccttattact gatggcgcaa tcaaagcgca tcagaagaaa ggtgtaagcc gcggcagggc
     4201 ccgtgcacgg atcgcaaaac ggtcatacgg ccactgcaaa ggagccggaa aaaggaaagg
     4261 tgcggccggt gcccgcaatc caagtaaaac ggcatgggtt cagaaaatac gggctatcag
     4321 aaaagtactc gttgaactcc gcgatgccgg aacgatcgac agccatatgt accggataat
     4381 ctatcggaaa gcggctggtg ggcagttcag aagtgttgcg cacatgaaag cgcagatgga
     4441 gatcctcgca gggaggatga agtaacatgg caacaggatc acggtatttt gttcctttcc
     4501 gaagaagaag agagggcaaa accgattatt acaagcggac cagactcgtt gttgcagatg
     4561 caccacgaat ggttgtgcgc aagacaaacc gacacatcat catacagctg gttactgcag
     4621 aacttgacgg tgatcggact ctcgtttcag cgaattcagc cgaactggag acatacggat
     4681 acaaaggatc gacatccaac acgccggcag catatctgac cggtatgctt tttgcggtaa
     4741 aagcgaagaa agcagattac agcagggcga ttctggatat cggtctcaac cgtgcaacac
     4801 caggcgcaag agtttttgct gcattgaaag gtgctgtcga agctggactg gatgtacccc
     4861 atggggagaa gatcctgccg tctgatgaac gtgccaaggg taaccatatc gctgcccata
     4921 acaaaaatgc agcagatatt gtctctattg ttgaacaggc ggcagacgcc ataaaaaagg
     4981 agctggtgta aatggcttat gaaagagagg agtggcgccc ggtcaccggt cttggcaaac
     5041 tcgtcgcctc tggagaaatt aaaagtattg accaggtact tgagagcggc aggccgatta
     5101 aggaacccca gatcgtcgat atgttccttc ctgatctgga agatgaggtt ctcgatattg
     5161 ccatgatgca gcgaatgacc gactcaggtc gtcgcgttca gtttcgtgcc gttgttatcg
     5221 taggtaaccg gaacgggtat atcggatttg gccagggtaa ggacgtacag gttggcaatg
     5281 cgatcaagaa agcgatcgtg aaagcgaaaa tgaatatcat taaggtccgg cgtggttgtg
     5341 gaagctggga atgcggatgt gatatcactc actccatccc aatgcaggtt gaaggcactg
     5401 ccggaagtgt ccgggtaact ctcaaacccg cacctcaggg tatcgggctg gttaccgggg
     5461 atatcagcaa aaaagtgctt gaactcgccg gtatcaagga tgtatggaca tttgcccgag
     5521 gacaaacacg caccacgatt aattttgcga aggcaacatt caacgcgctc aatgagacca
     5581 acatgatcag gacagggagg tcactgtaaa tgtacgcagt cgtacaggtt cgaggcgttg
     5641 ttaagactcg ccgggacatc aaggatactc tcaagatgtt gcgtctgcat cacatcaacc
     5701 attgcgtact tgttgctgat acacctgaaa accttggcat gatccgaaaa gtaaaggatt
     5761 atgtagcata cggtgaagcg gatgcaccaa caattgaaac tcttctgctg acccgtggaa
     5821 gggttattgg tgatgccccc ctgacagacg agtacattaa atcaaactcg acatatagca
     5881 gcatttcaga atttgcacag gcacttgcaa gtggtgagac aaaattgcgt gaaattccgg
     5941 gactgaaacc cgttttacgc cttcatccac cacgcaaggg atacaagacc accaaacgta
     6001 ctttccctca gggaggagcg cttggctatt atggtcagga gatcaatact ctcctctata
     6061 agatgaggtg atgcaggatg ccaaccaaca aacgttcaaa gtaccgcgga tcgcggacat
     6121 gcggtggcgg cacccataaa aaccggcgtg gtgcagggaa ccgcggcggc cggggacgtg
     6181 ccggaattaa cgcgcaccac tttgtcaagt ggtataaaga gatgggagga ccagtctttg
     6241 gaaaggacgg tttctcgaat agctcccaga ttactgtcac ggtaatggat gtcggaatta
     6301 tcgaccagat aatcccttct ctgttagcgc agggtattgc aaagaatgaa ggagatgcca
     6361 tcgtgatcaa cactgctgat atgggcatca acaaggtgct gggcagcggt aaggttacca
     6421 aaaagctgaa tatttccgcg caggcatttt ctgaatcagc caagataaag atcgagaaaa
     6481 tgggtgggaa gacgcaaatc gtctgaccat ttccgtgatt tttggtgaaa caatgggaaa
     6541 cctgctggat cgaatggaac ccctgctcgc tgcaatgcct gcagtcaaaa gtcctgaagg
     6601 acacgttcat ttcaagaata agttgatctg gacactcgga attttaattc tgtattttgc
     6661 cctgacgaat attccggtct ttggcctcgc gccctcaaac caggatttgt ttgcagcatg
     6721 gcgggctctt cttgccggcg caagcggctc gatagttcac cttggaatcg gaccaatcgt
     6781 cacagcatcc atcgtgctcc agctgctcaa aggagcagat attctccata tcgatacaag
     6841 cgaaacgcga ggacaggtca tgtacatggg tctgcagaag atccttatca tggtcatgat
     6901 cgtaatcgaa gctgcaccaa acctcgttgg gagcttcatg atacccgatc cggttattgc
     6961 caaccagttt tttgggggta acctgtttgc ggtctctcta atcattttca tccagatctg
     7021 tatcggcggg gtcctcatct tcttcatgga tgaagtagta acaaaatggg gtatcggttc
     7081 cggcgtaggt ctcttcatca ttgcagggat atcacaggca atagtgaatg gtttcatcaa
     7141 ctgggcaccg gtcgccgacc agtatccggt cgggtttttc ccacgcctca tcgccattgg
     7201 tcttgacggt ggaagctatg tcacgtactt tggaaaagat attctcgcat ttgtcaccac
     7261 gattgcaatt ttcctaatta ttgtgtacgt ggaatcaacg cgcattgaga tcccgcttgc
     7321 ccacgcacag gttcggggtg cacgggcccg gtttccggtt aaattaattt acgcaagtgt
     7381 cctgcctatg attcttgtcc gtgtattaca ggcaaatatc cagatggtgg ggatgttcct
     7441 ctcaaatgtc aatatcacca tcttcggtgc attcgaagga tcgcaaccaa ttaacggtat
     7501 aatgtactat cttgcaccga ttaatggacc ctcggactgg atgtggtgga ttactgatct
     7561 cgggcatgct ccatggcagg ttctattacg tctgggtgta gatatcacat tcatggtggt
     7621 cggaggtgcg attttcgcgc tcttctggat caaaactgcc gggctcgatt caaaggatgt
     7681 ggcccgtcaa atccagctct cgggcatgtc cattcccggc taccgaagga acccgcaggt
     7741 acttgaaaag tatcttgacc ggtacattcc ccgcgttacg gttattgggg gtgtcttcat
     7801 aggtatactc agtgtaattg caaacctgtt tggtgtcatc ggtgctgtca ttggaaccgg
     7861 gttactcctg acggtcagta ttacctacag gctctatgag gagatcgcaa gccagcaaat
     7921 catggaaatg tacccgttca tgcggacatt ctttggaaaa gaataggacg tggaagggaa
     7981 acaggatgga aggaaaaaag gtcatcataa ccggagtgcc gggcgttgga aagacaacgg
     8041 tcgtcaacga agcactaaaa aaaatcagtg aagaaggcgt cgaataccaa tcaataaatt
     8101 ttggcacctt tatgtttgaa gtggcaaaaa atgataatat tgtccaggac agggatcaga
     8161 tgcgtacgct ggatcgtgca gtccagaaac aactgcagca gcgtgctgca caggcaattt
     8221 cccggatccc cggcaatgtt ctgattgata ctcacgcatc tgtcaagact cctaaaggtt
     8281 accttgcagg acttcctgag tgggtgttac gcgagattat gcctgattgt attgttcttg
     8341 ttgaaacgga tgatgaccaa atcctcatgc gccgtatgac cgatgaaaca cgttcgcggg
     8401 acaaagaggg ttcacgatcg atagctgaac accagcaatt taatcgctcc attgctgctg
     8461 cctatgcaat gcttaccggt tgcacggtga aaattgtcat aaacgccgat ttcctcctcg
     8521 aacgggctgc cgatgatatg gcagcagtac tcaggtgaat acatggattt tggaaaaata
     8581 tgggataagt acggcattta tattgcactt atgttcatga tgctgatgat gttttcatac
     8641 agcatcgagt ggttgcgtgt aggggtggga cagggaattg atgttgtatt tgcacccttg
     8701 ctcgactggt ttaagatccc attttatatc ctaattgtta tcctctctgc acttaccggg
     8761 ttatactctt caattatcca gaagtatacc atcgattacg agaagatgac cggggctcag
     8821 gaaagaatga aggaatttca gaccgagtac cgcgaagccc agctctcaca ggatgagaag
     8881 aagatcaaga aacttgaagc aaaaaaagat cgaataatga aagaacaact tgagatgtcg
     8941 cagaaccagt ttaaaccaat ggcttatata ctggtgctga ccgttcccat ctttttctgg
     9001 cttctctttc gcctcggcca ggttaagagt acgataaccc tgccctacgc tggcaccatc
     9061 gggcttcacg acccggctct ctggattatc ccggcatgga tcctctggta tatgatctgt
     9121 tccattacca taagccaggt aatacggaaa tccttgaaca tcggtgggat ctgatgcgaa
     9181 tcaccgtgag cgggcttccg ggaagcggta caacctcact ctcacggtat ctttcagaac
     9241 gccatgggtt taccatgatc tctgccggag aggtctttcg cgacctcgca aaggagcacg
     9301 caatgggatt ggcagagttt ggtcgcctgg cacaggagaa tccggcattt gataaaatga
     9361 tcgatgcccg gcagaaagag atcgcagagc agcgtgacaa tatccttgtt gaaggacgtc
     9421 tgtctggctg gatggttccg ggcgcgaatt taaaaatctg gctcttcgca ccaattggct
     9481 gccgtataaa gagaattgtt ttccgtgatc agatagagga tgaaaagacc gcaaaacgga
     9541 taacaatcga gcgggaggag tgtgaagcgg gacggtatca cagctattat aatatcgaca
     9601 tcaatgatct ttcaatctat catattatcc tgaattctga gcactggagt gttgaaggac
     9661 tcggttcaat agttgatacc gcgatcgcac agcttaatcc ttcggataaa cggtaattct
     9721 tgatgttatt gtgttgatgc taaaaaaaaa ttattccgta ttcttttaat tattccggca
     9781 tgaaaaatcc tttagccagc aagagcgaga atgattactg tggaaaaaca gattacaatc
     9841 cagcttgttc attcatggga tgaatatgag attgctgaat tataccgcgc cggcgggtgg
     9901 tggaaggagg agtataatga aagcgatctt cgccatttga tgcggggaag ctacctgttt
     9961 gccgtggcag tcgacacaaa aacaggtcgg gctgtaggta tgggcaggat aatatccgat
    10021 ggattttctg atggatatat tcaggatctt gttgtgttgc cggaataccg gaaaaccggt
    10081 attggcggtc ggattgtagc tgcccttgtt aataaatgcg ttgaacaggg aatctcatgg
    10141 atcggtctga ttgcggaacc aactaccgaa aaattctacc tgccctatgg atttcagcgt
    10201 atggaaggac acactccgtt attattccgg agggatttat agtgcttacg caagaaaatt
    10261 ttaagcctgt taccctggaa gacagggctt ttttcgaacg acactatgca cagtatcctc
    10321 agacgcatag tgataataca tttaccaata tggtctgctg gaatcattac gcgcactaca
    10381 aatatgccta tgtggaaaaa aatatcatca tagctagtac aattgatgat attacccggt
    10441 tccgtccccc aatcgggcca cgcgatccag ctcttttaaa atcgctgata cgccttgcat
    10501 ctgatgtcag tgacaatgaa cctattgtgt taattgatcc ggaaactgcc agctggatga
    10561 gggatatatg tcccgggatc aacctcgttc ccgatcgcaa tcattttgaa tatgtctacc
    10621 gatcagccga ccttgcagat ctccctggta aaaattacct cacgattcgt caccagatta
    10681 acaagttccg tcgcaattgt tcaagctcgg ttgagccgat cacaaaagaa aactgggagg
    10741 aagtaaagcg attccttatc gaatggtgcg aatggaaagg gtgtgaaaac gataaagtcc
    10801 ttgcacatga aaaggaagcg gttttctttg ctattgatca ttttgacgca ttatcgatga
    10861 aaggattgat tatccgcgtc ttttcaaaaa tcggtgcaat gtcactcttc gaaccactta
    10921 acgccgatac tgcattggta catttcgaaa aagggctgcc ggattgcgaa gggatttaca
    10981 aggtaatcaa tgctgagact gcggcatttc tgataaaaaa atattcctac cttaatcggg
    11041 agagcgacct tggtatcgca ggtcttcgtg aggcaaaaat gcgatatcat cctcaccaca
    11101 tggttgaagt ctattcgctg aagcgggatt cctgaattat tccgaattta atacctgaat
    11161 tgactggtaa gcgatcagac aggggcgatt ttatagaaga aaaagattgc aggaatatgg
    11221 tttctttaaa attataaaaa aagggtaaag tgttaacaca gcccctaatt tttttatttc
    11281 tccgacgaga tttttgaaaa attttcataa ccaaaactga gataaaaaaa gtaaccgtga
    11341 ttttgccggt atactttttt gtgttgtgaa aactttatgc ggatctatcg ctgcgtgggg
    11401 gtcgccacag cggcgggggc ggaggcttta ccgaagcccc caggagcgtc agaataattg
    11461 atccgagttg tattatctga agattttatt ttaaaaattt ggggtaaaaa tagcacttta
    11521 ccctgaaaaa aatgtgagat gtattaaacc aatgctaaat aacccagaat tcccgatgca
    11581 ataattttaa ctgcgatagc agcaaccagc atgccaagga ccttacccat aatatcagtt
    11641 acgacctctc ccagtacacg ctgtatgttc tccgcataat agagaacgag ccatgtcgca
    11701 ataagcgaaa aggtgatcgc aacaaagggt atgaggactc cataatctct tgaaagaagc
    11761 ataaccgtgg taatagtacc tggcccgcat aaaagtggcg tgccgatcac tacacctgca
    11821 gctgttttgg catgttcttt gtaatgcccg aactcaatac cgagcgcttc ctggattcca
    11881 agaaggaaaa gcagtatacc gcccgctatc tggaagctgg gcatgtttag tcccagaata
    11941 tcaaagatca ttctattgaa aatcaggaaa aggtacatca gtcccccggc aacagcgact
    12001 gcgataaaag cctgtttatg aatttctgcc ggtgtctgac ctttagtcat tgctgcaaat
    12061 attggcacag acagaagtgg atcaaggatg atgaaaagag cggcgaacga ataaataatt
    12121 ccggacagta aatcctgcat ggtctgatgt atggtatatg ctctgattat ggataaaaat
    12181 gagaggtctt cttattgagt gggttttaga ttccctggtc atgcgataaa atatactata
    12241 agaattaaag atgttgagat tgcaccagac gccgatataa ggcgggcttt ggtatctttt
    12301 acgagcaccc atgaggcaag acagaaaaaa atgtacggaa tcagcgtagt gatcaccgaa
    12361 atggttgcaa gctgatcgaa atggttggaa aagagcaaga gacccccact gacagcacaa
    12421 cctgtaataa gtgcaggagc aggagttcct ttggaattta aattcctgag taccgggata
    12481 aaaaattcag aagacattga ctggattacc ctggacgtac ctactatgta ggcgtttatt
    12541 gccgagagca tggccactat accgatacag gcaattaatg tgccggattg tgtcagaagt
    12601 attccggcag cagttgccac aggggcgggt gaagcagcaa gggcttctga accaacactg
    12661 ccgatgagtg aaacattcag aaggaggtaa agggtaacaa caattcccat cacgatgata
    12721 agggatcgcc ggataagcgc cgggtcccgt gtttcctcaa cgggaatagc actaatctca
    12781 aaaccggtaa acggccagta tacaatgatg atcgttgcaa gcaaaccggt gaccgtgagg
    12841 ggggtcgtgg gtgaaaaatt tccaacgttt ataaagggga tgagcaggaa cgcaatcatc
    12901 accaaaggga tgattttaag ggtagtcaga atattttcgg tcattcccga aaatgatatg
    12961 ccggtaatat tcagccagca aaaggcaccg ataatgacaa tttcgataag aagtacattt
    13021 gaaacaccaa accaggttat gtactgacca ataccggctg caatggttga aactccaaac
    13081 accgaagaaa taaggtaaag gataatgagt ggaactgcaa aacgggcacc gaataccgat
    13141 ttaaacagaa tatagaaact atttgatgaa gtaaattgtg aagatacata ggcaagcgac
    13201 aggagtacac aggacgcgga cagggccaca atcacccatg caaggagtga ccaggaaccg
    13261 gtcagctttg ctgcgacacc aggtactacg aaaataccag aaccgatggt ccctcctacg
    13321 ccgagactga caagttcaaa taacccaagt gatcgtttgt acgaacctgc ttccgacata
    13381 tttacccgtt aatatttgca ttaaaccaaa tttataatag caggtagact ctatcttcag
    13441 ctatacctga ctcttctgac gaaaaacgag atggacgagg agtctcataa aggtgtgatt
    13501 ttcatatggc aaaaactgta gaaaaggcaa aaaagagcat ggggataaaa gcaaaaaaac
    13561 cagctgcagc taaaacgaaa gccgcaataa aaaagccagc agcaaaagga aaaaagggtg
    13621 ggccttcaaa aaaagaaggt acccaaatga ggtacaaatg caggatctgc gggtatgtct
    13681 attctcctct tcgtggggag ccgcataatg gaattcctgc agggacaccc tttgaggatc
    13741 ttccggaaac gtacctttgc ccggtttgtg gatttcaggg aaaaggaaag gtaggtaagt
    13801 ggggctttga cgagtggcgt ccgacccgct atatctgttc catgtgcgat tacatttacg
    13861 atgaaaaacg tggtgaaccc caccgcggta tcaaacccgg cacaaaattt gaggatcttc
    13921 ctgaagacta tgtatgtcct gtttgtgcaa cggatccaaa aattgtgtta cgatacggaa
    13981 atgttctcaa gcagggcttc gcaccgttgt atgagtaaca ggacagactt tattcttttt
    14041 ttaaaaatta ttaatccatt attccattat cggtttgcag cagtctgtca ctcaccgata
    14101 ctattcttac acgaaatttc ttttccggtg tatcatgatt tttcattagc agatccatat
    14161 gcttgccggg agtttataac taaaaaaata tcataccttc ccatatttga gtttgcgact
    14221 gaaaaatcag gaaggtatta tgagaccggg tgtgaatgat aatctgagag tgtatggaag
    14281 ttcatcatca attagctgag aaagcccatc attttatgag aatcggacga tatgatgaag
    14341 caattgatct atacaaaaaa ttactagaat ctgaccgtaa gaacacgaca tatcttgaga
    14401 atatcggaaa aatgtattat tcgcttggca gacatgagga tgctattgcc tggtacgaca
    14461 aggcggttgc catcaatcct aaacttcctt cggtctggtt cgaaaaggga tatgcactgc
    14521 gcaaaattca ccgatatgaa gaagcaataa tgtgctttga ccaggttctt actctcgatc
    14581 cggacaatgc tctcgcacgt gccaataagg gatacgcatt aaacgagtcg ggaaaattca
    14641 gggaagcact tgaatctttt gataccttgt taaaaacaag tccgaaaaac ctccgggtta
    14701 tgacggcaaa aggcatctca ttatcgaaac tgggaaacaa taccgaagca cttgagtatt
    14761 tcgataaagt gatttcgtac aatcctctgg actattatgt ccagaatcaa cgggctgcgg
    14821 ttctccggaa ccttggccgg atacaggaag ccgatgaatt tgtcaagaaa ggaaaatagt
    14881 aatcctaata agaaaattct aaaaaactgg tcaagaatgg aagttactga aacctctctc
    14941 cggtcaaata aaaaaaccgt atagttcttt taaaatcaga aatcctgcat caagcgaaaa
    15001 aaaattagat aagtttgaag attggatggt tttctgcctt gcactcgata cccagttggc
    15061 gggtagcctc aaggacaacg atatccgtgt aagcttgtgc ggtgatcatt gcttctacgt
    15121 tctcgatagg accgtacatg atgaggtctg cgccgagcgt gcttgcaatc atgttgcagc
    15181 cgatatcggg ggctgaccat gctgcctgct tgatgccttc cataccgccg aggtagtggt
    15241 gagacatctg cttgagaaga acatctttac cctcgtatcc tgctgcgagt actgaggggg
    15301 ttttgctcgt ccccttccag cgcttgagcc aggtccagga aacggtcatg ttgtggtatg
    15361 caccaccggt cgggtaaccg tggattgcct tacaggcaag gatgtcacgg tatgatccac
    15421 ctgagccaag accgagcggg gtcgctgcag tgtcgagaat cgggcgcttg ataccacaat
    15481 actctgcaat ctccagcata cccttggtct gcccggcaac gccaccctct acgagcacct
    15541 tctccctgcc gacaacagat gggtcagccg ggttgaaggc gaggacgatg gctgcatcga
    15601 cgtcactgtt cttcagcgct tcgatgctct cgggcatgat ggaaccgttg atcgagttgt
    15661 agatcgcacg gtgtgcaagt ccggtatcgg tcacgtactt gcatgcatgg gcaagtgcct
    15721 tgggaaccga tgagtccatt aggaatgcag tcttgttgtc aatactgtcg aaccagctga
    15781 tgtagctctc gaatgcttct ccgtattccg caataatctg gataaagtgc gggataccgg
    15841 tcaggtcaga gaggacctga cagcggttcc agagggcttc tgcttttgcc ttgtcgatct
    15901 ttcctgtctt gtcatcaagc acaacttcat gcttgttata gaagattgag gcacccaata
    15961 ccgtcgggta ctccccaggc tgtccaccga tcatggtgcc gttgaagtcc cagacactct
    16021 gttctttttc aaatttgaac atgttatttt acacctcctt ataccaatgt catcaatttc
    16081 tggtacaaaa acaacagcat cataaatacg gttaatcctg ctaccaggcc gtacagtatg
    16141 ccgatatctc gtccgatttt tctcccaaca cgctgtgcaa tctcagcatc cacgaactcg
    16201 atcttcttct caatcttatc gagtctcttc tcaatttcga ggaactgggg gtttgcacct
    16261 gcggcagccg tctcaacgcc tccagcagct tctttgacct cgacaacgat agcatcagca
    16321 gcgaacgcac ccgggtcacg ggctttcagt tcgttgattt ttgccttgat tgcattgagg
    16381 tcttcgcttt ccatgatgtt gacgatttcc gtctgttcct ggaagcgctt gatcgctgcg
    16441 tcattgaggt tctcgatgaa gggaatagca ccttcagcac cgacaaccct gccttccttg
    16501 acaccgcctt tgtggagtgc cccgaatgtc tggcctgata ggtggccttt gacttcggtt
    16561 ccgcagaaaa gcatgaacct gatgttgggg ttggcgataa cattggcgat gactttttca
    16621 agaccgaggt tttcagtctt acaggaaccg cacaatgcag cgcccgcgtc acatatgcct
    16681 ttttcgtcga ggtgtgagcc catggtgaca acagccaccg ggctgttagc gtc
//
LOCUS       DASRLV010000135         2340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1179189,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000135 DASRLV010000000
VERSION     DASRLV010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179189"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..74
                     /locus_tag="VFC43_05445"
     tRNA            1..74
                     /locus_tag="VFC43_05445"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Val,seq:cac)
     gene            complement(137..724)
                     /locus_tag="VFC43_05450"
     CDS             complement(137..724)
                     /locus_tag="VFC43_05450"
                     /inference="COORDINATES: protein motif:HMM:NF025865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZK30663.1"
                     /translation="MKECIRELVKKSRSYRRFDEQRSIGRDTLIELVDIARFCPSARN
                     HQPLRYVVSTDPKQTARICDCLLFALDLPDWGGPVKGERPVGYITILTSEKYTPYTGH
                     DIGIAAQTLLLAATELGFNGCMFGSIKKKELQSVLSLPENYEIQLVLAFGYPAEEVVL
                     EEVDKDGKTNYWRDSKGVHHVPKRALAEVLIVADP"
     gene            939..1196
                     /locus_tag="VFC43_05455"
     CDS             939..1196
                     /locus_tag="VFC43_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZK30664.1"
                     /translation="MQVTPSIHAIRHTFMIPLGSGVGLDRFVYSFLVYGDRITLIDTG
                     VAGCEKDIFDYIRSTGRNPSEIALIILTHSHPDQHRGSPGN"
     gene            1216..1818
                     /locus_tag="VFC43_05460"
     CDS             1216..1818
                     /locus_tag="VFC43_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZK30665.1"
                     /translation="MAAHPAERTWIEDVDLQNRERPVPGFATLVGEHVSVDHELADGD
                     TVEPDEKRGSEILVFHTPGHSKGSISLFLHSEGALFCGDAVPVAGDLPIYEDAAASVQ
                     SVKRLRGLKGIHILLSSWDEPRTGDTAYQQMDMALDYIQKIHTAVLSSAGDCSDDLMV
                     LTQKTVASLLLPPHAVTPLLARTFAANLSIRDKKNLFPDS"
     gene            complement(1836..>2340)
                     /locus_tag="VFC43_05465"
     CDS             complement(1836..>2340)
                     /locus_tag="VFC43_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30666.1"
                     /translation="LNLSVVFATIAFSWDDPDLHYPSGLRELAARLPIAHHLACRVQV
                     GCRWILVDATWDSPLSNAGFPVNDNWDGYSETRCAVKPFKSLVQEVLCNKTTHDPYRV
                     KDKAELCPVDDEKGHWAAEDREQYYREKVAVRTPVERKQIVKFSHDFDAWLVEVRNKG
                     IPDFTKK"
BASE COUNT          553 a          618 c          561 g          608 t
ORIGIN      
        1 gggctcgtgg tctagttggc tatgacgtcg ccttcacacg gcgaagatct cgtgttcgaa
       61 tcacgacggg cccactcggt tttatacgct tttatataat tcaaggctca ttttttaaaa
      121 atctcgtagt attcaattac ggatctgcca ctatcaggac ttcagcaagc gcccgtttcg
      181 gaacgtggtg aacgcctttg gaatcccgcc agtagtttgt cttcccgtct ttatcaactt
      241 cttccagaac aacctcttct gccgggtacc cgaaagccag cacgagctgg atctcgtaat
      301 tttcaggtag tgagagcacc gattgaagct cctttttttt gattgacccg aacatgcaac
      361 cattaaatcc gagttcagtt gctgcaagca gcagggtctg ggcagcaata ccaatatcat
      421 ggccggtata aggggtatat ttttccgatg taaggatagt aatgtagcca accggtcgtt
      481 ctcctttgac aggtcctccc cagtcaggca gatccagggc aaacaaaagg cagtcgcata
      541 tccttgccgt ttgcttcgga tcagttgata caacatatct gagcggctga tgatttcttg
      601 ccgatggaca gaaccgtgca atgtctacaa gttcaatcag agtatcccgg ccgatggatc
      661 tttgttcatc aaatcgccgg tagctccggc tttttttaac cagctcccgt atgcactcct
      721 tcatgatttc tatttggtgc ttgcatcaaa aaagcactta gagctgcggt aactgtagta
      781 gttcattttc aggtccttgt cggaaaaaaa ccggtctttg gtaacggtat cattcgtgaa
      841 acattgactc tccatcaatg aaccggggcc cggaatactc cagctgcccc tcacaatata
      901 tccttcactg ccgaatattc aagaacatag gagatcatat gcaggtcacc ccttccatcc
      961 atgcgatcag gcacacattc atgatcccac ttggatcagg tgttggtctc gaccgttttg
     1021 tgtattcatt cctcgtttat ggagaccgga taaccctcat tgataccggt gtcgcgggtt
     1081 gcgagaaaga tatttttgat tacatcaggt ccaccggccg taacccgtca gagattgctc
     1141 ttatcatcct gactcactcc catccggacc aacatcgggg cagcccgggc aattaagcag
     1201 ctgaccaggt gcagcatcgc ggcacacccg gcagagcgga cgtggatcga ggatgtggat
     1261 ctccagaacc gtgaacgtcc ggtgcccggg tttgcaacac ttgttggtga acacgtatct
     1321 gttgatcacg agcttgcaga tggagatacg gttgagcctg atgagaaacg gggaagtgag
     1381 atcctcgtgt tccacacacc ggggcattcg aagggttcga tatcgctctt cctccacagc
     1441 gaaggtgcgc ttttctgtgg ggatgcagtc ccggttgcgg gggatcttcc gatctacgag
     1501 gacgctgccg catctgtgca atcggtgaaa aggctccgtg gattgaaggg gatccacatc
     1561 ctcctctcat catgggacga acccaggacc ggggatactg cataccagca gatggatatg
     1621 gcgcttgatt acatccagaa aatccataca gccgttctgt cctccgcagg agactgttct
     1681 gatgacctga tggtacttac gcaaaaaact gtagcatctc tcttacttcc ccctcacgca
     1741 gtcaccccgt tactggccag aacttttgca gcaaacctct caataagaga caaaaaaaac
     1801 ctgtttcctg attcttaagg ttcggggctg aggaactatt ttttggtaaa atccggtatc
     1861 cctttattcc gtacttcgac aagccatgcg tcaaaatcat gggagaattt tactatctgt
     1921 tttctctcaa ccggtgtgcg gacagccacc ttttcacgat agtactgttc ccggtcctct
     1981 gccgcccagt ggcctttctc atcatcgacg gggcatagtt cggccttgtc ttttacccgg
     2041 tacgggtcat gggttgtttt gttacaaagc acctcctgca ccagcgattt gaacggcttc
     2101 acggcacatc tcgtctcaga atacccgtcc cagttatcat tcaccgggaa cccggcattt
     2161 gaaagagggc tgtcccatgt cgcatccacc agaatccatc gacaacctac ctgcacgcgg
     2221 caggcaagat ggtgagcgat cgggagtctg gccgccagtt cacggagtcc agaagggtag
     2281 tggaggtccg gatcgtccca tgaaaaagcg attgtagcaa aaaccacgct taaattcagt
//
LOCUS       DASRLV010000136         5526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_370562,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000136 DASRLV010000000
VERSION     DASRLV010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370562"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VFC43_05470"
     CDS             <1..514
                     /locus_tag="VFC43_05470"
                     /inference="COORDINATES: protein motif:HMM:NF020514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1919 domain-containing protein"
                     /protein_id="HZK30667.1"
                     /translation="YQTPFIGCFLHAPCYIQLVKNLKEYLFSELSFKKESKYSITNKL
                     RSENGNYYPIGVLKNDVEIHFIYSKSEKEAETIWYRRRNRVNMENLFFSFTDRDFCTL
                     SILEEFDNLPYPQKVVFTAKNYPQIKSSVWIKDCREEPFVPDLFTNAYLYKKYFDVAD
                     WLNGGKGKKN"
     gene            complement(615..1550)
                     /locus_tag="VFC43_05475"
     CDS             complement(615..1550)
                     /locus_tag="VFC43_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZK30668.1"
                     /translation="MKYIVTGGAGFIGSHIVEAIAGSHEVVVIDNYSSGKPENLSMFS
                     DNVRQIKGSITDLPLLREAFSGAEGIFHLGAIASVSRSIDDPGATHETNLTGTLNVLL
                     AARDCGVKKVVFSSSSAVYGDEPTLPKREDMPPIPLSPYAVSKLAGEYYCKVFFELFG
                     VQTVSLRYFNVYGPRQDPQSEYAAVIPKFITRLLNHQAPLIFGDGKQTRDFVYVKDVV
                     QANLLAMQSTKTGIFNIGSGESLDLNTLAGTLAEIMDVNIPPVYEKPRSGDIRDSVSD
                     ITAAKKSLGYRTSFSLDRGLEETIMWFRHKKNHQV"
     gene            2176..5349
                     /locus_tag="VFC43_05480"
     CDS             2176..5349
                     /locus_tag="VFC43_05480"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HZK30669.1"
                     /translation="MYSIDYITSIEYLIAKHKVVKFVDYSIIPPSEKSTSHTPNSWNP
                     ESAHSSVMGVLMLIAIVMIMGGVVAFVFTSQPLPDKVPMAYLGIAKSVDGVELINKAG
                     DTLTSSSVTILVDGIDRTSEFRTQENTPGWGTLKAGERIHYTSPVKPESIQIVYASNS
                     GRYLLASSESTTYTPIKIGSTQTTPSQAMQVASPPTQVTATATVDAIFLSSTIPLTMN
                     SSQKYPVSVTMKNTGSMTWDEASQIRLGAVGDTSGDSYKCGSTRIQIPGGTRVLPGEK
                     YTFVFTMTAPATPGTYHPDYQMVWSGHYWFGSKATRTLEVVSNPTVNAQVISSTIPSS
                     MNAGQKYSVSVTMKNTGSMTWNETSQIRFGGVGDTKGDAAKFSPIRIQIPAGTSILPG
                     EKYTSTFTMTAPAKPGAYRPEYQMVWSGHYWFGARASRTLEVVSTSAPVAKFTSDTMQ
                     GQSPLTVQFIDQSTGAAPLTYHWDFSDGEGNLPENSYQNPSWRFWEDVATSYTVTLTV
                     TNAFGSDTIVKQNYIRLGDTLSPAPVAAFTSSVQSGTAPLTVQFTDQSTGTAPISYAW
                     DFNNDRAIDSTAKNPAFTFVTDGDYTVNLTVTNADGKDTDIKTNYIRVTTPVITSVPV
                     AQFTANTTGGTSPLTIQFTDKSVSTGTTSYKWDMNNDGIVDYTTKNPSHTYTTAGSYT
                     VKLTVTNASGSDNEIKTNYITLSSLQAGDCYGAEACNPTGNPLGGGAGYTRIITEKDS
                     RVKYVVSTRDQLVTALKNAKSGEVVFVKGTAVIDMTGTPNNIIPDGVTLASDRGNGGS
                     TGALIKYTSNKNGRYGTSLFVAGGNNVRVTGLRLEGEMLPQDGTGNGEANYLVAIIVR
                     NKAGLEVDNCEIRGWAWSCVTTMDSTGTYIHHNYLHHNQARGEGYGADIYGGDMLVEA
                     NIFNYNRHDIAAGGYPGESYEARYNLVRGDGHPIGSHHFDVHACNPNNYADDSETSPA
                     FIAGHLYKIHHNTFEAGQQASIGIRSRPVTGAYIYNNIFMSASTDTAGGVPVWQRAVT
                     WGNMFVTNNYWKGTLYTGDQIVWYLHP"
BASE COUNT         1538 a         1341 c         1278 g         1369 t
ORIGIN      
        1 gtaccagacc ccttttatcg gttgttttct ccatgcacca tgttatattc agctggtaaa
       61 aaatctgaaa gaatatttat tttcggaatt atcattcaaa aaagaatcaa aatattcaat
      121 aactaataaa ttacgaagcg aaaatggaaa ctactacccg attggagtat taaaaaatga
      181 tgtagagatc cattttattt attctaaaag tgaaaaggaa gctgaaacga tatggtatcg
      241 ccgtagaaat cgggtcaata tggaaaacct gtttttttca tttaccgacc gggatttctg
      301 cacgttatca atccttgaag agtttgataa cctcccttat cctcaaaaag tggtatttac
      361 agcaaaaaat tatccccaga tcaaatcttc cgtctggatc aaagactgcc gggaagaacc
      421 ctttgtgccc gatctcttta ccaatgcata tctgtataaa aaatattttg acgtcgcaga
      481 ctggcttaat ggcgggaagg gaaagaaaaa ttgaatttgt gaagatcccg gtttttttcg
      541 caagcggagt ttttggagat accggattgc ggatctgacc attatcgtga ggcaagatcc
      601 ggtccttagc tccactacac ctgatgattc tttttgtgac ggaaccacat tattgtttct
      661 tcaagtcctc tgtcaaggga aaaacttgtc cggtatccca aagatttttt cgcagcagtg
      721 atatctgaaa ccgagtcgcg aatatccccg gatcttggct tctcatatac cgggggaatg
      781 ttaacgtcca taatctctgc aagagtacct gcaagggtat taagatccag gctttccccg
      841 ctcccgatat tgaagatacc ggttttggtg ctctgcattg ccagaaggtt tgcctggaca
      901 acatccttga cgtatacaaa gtcacgggtc tgtttcccat ccccaaaaat caaaggagcc
      961 tgatgattga gaagacgggt gataaatttt ggaattactg ctgcatattc agattgaggg
     1021 tcctgtcgtg gaccatacac attgaagtac cgtaacgata ccgtctgaac accgaataat
     1081 tcaaaaaaaa ccttgcaata gtactccccg gccagcttgg agactgcgta cggggaaagc
     1141 ggaatagggg gcatatcctc gcgctttggg agtgtcggtt catcgccata gacagcggaa
     1201 gaggaagaga agaccacttt ttttaccccg cagtccctgg cagcgagaag gacattcagc
     1261 gtaccagtga gattggtctc gtgcgtagca ccgggatcat cgatggacct cgaaactgag
     1321 gcgatcgctc caaggtggaa aataccctca gctccagaaa aagcttccct gagcaaagga
     1381 agatcggtaa tacttccctt aatctgacgg acattgtcgg aaaacatgct caggttctcc
     1441 ggcttcccgc ttgaatagtt gtcaatgaca acaacttcat gagaccctgc aattgcctct
     1501 acaatgtggg atccgataaa ccccgctcct ccggtgacaa tgtatttcat gatactaccc
     1561 ggatcactca catgatccct gaaaaataat caatgctgat ataatatact ttgaaggcat
     1621 gaaatcttaa attattggta gttgtgaact tattattatt tgaccacagg aatgttgtgg
     1681 atcgaaaaaa ggggttgaat ccccatccac cctggtcagg accaggatat ttttcacatc
     1741 aaatcattga caggaagagt ttgccgtcag gctcctctga attcaggcaa atatctttaa
     1801 tccggctctg cggaaaaact tattttgtgc atatgttctt tgattttaaa aaatacgaag
     1861 ctggactccc tctttccccc ggtgtgggag gcagcgagtg tatttccttg accccccttc
     1921 cggttaacct tatgagaggt aatcgcatgg tcgggagacg cccgtttggc gggggcggag
     1981 gtgagacccc aagcctccaa gagctcggtg atttattttt tcttgagctt ttcaccagga
     2041 ccttaattct ccaaattttt ataaagaaca gctaaccctg aggttagatc aatttaccag
     2101 ttgattacca gattgcactc aaaagggtgt cttaatttat aatatatcaa tgagaagcat
     2161 ttatgtaatc cattgatgta tagtattgat tacattacat caatagaata tttaattgca
     2221 aagcataagg tggtgaaatt cgtggattac tcaataattc ctccctcaga aaagtcaaca
     2281 tcacatacac caaatagttg gaatcctgaa tctgctcact cgagtgttat gggtgtgctc
     2341 atgctcatag caatagtgat gatcatggga ggtgtagtcg cattcgtttt cacctctcag
     2401 cctttaccgg ataaggtacc aatggcatat ctgggaattg ccaaatccgt tgatggggtt
     2461 gagctgatta acaaggctgg agatactctc acaagctcat cagttaccat attagtggat
     2521 ggtattgaca gaacatctga gttccggaca caggaaaata ctccaggatg gggaacattg
     2581 aaagctggtg aacgcattca ttatacaagc ccggtaaaac ctgaatcgat tcagatcgtc
     2641 tatgccagta attcaggaag atatcttctt gcctcatctg aatccacgac ctatacccca
     2701 ataaagatag gttctaccca gacgacaccc tctcaggcca tgcaggtcgc atctccaccc
     2761 acgcaggtca ctgcgacagc aaccgttgat gctattttcc tttcatcaac gataccatta
     2821 accatgaact ccagccagaa atatcctgtt tctgttacca tgaaaaatac cgggagtatg
     2881 acatgggatg aagcaagcca gattcggctt ggtgctgtgg gtgacacaag tggagattca
     2941 tataaatgtg gatcgacccg catccagatt cccgggggaa ccagggtctt acccggggaa
     3001 aaatatacat ttgtctttac catgactgcg cctgccacgc caggtaccta ccacccggat
     3061 tatcagatgg tgtggtccgg ccactactgg tttggttcga aggcaacaag aacccttgag
     3121 gtcgtgagca acccgacagt taatgctcag gttatatcga gcacgatacc atcatccatg
     3181 aatgccggcc agaaatattc tgtgtctgtt accatgaaaa ataccgggag catgacatgg
     3241 aatgaaacaa gtcagatccg gtttggcgga gttggtgaca cgaagggaga tgccgcaaaa
     3301 ttcagtccta tacgcattca gattcccgcg ggaaccagca tcctgcccgg ggaaaaatat
     3361 accagtacct tcaccatgac tgcgcctgcc aagccaggtg cctaccgccc ggagtatcag
     3421 atggtatggt ccgggcacta ctggttcggt gcccgtgctt caagaaccct tgaggtcgta
     3481 agcacatcag cacctgtcgc aaagttcacg tcagatacta tgcagggaca atccccgttg
     3541 accgtgcagt tcatcgacca gtcaaccgga gccgctccct taacatacca ctgggatttt
     3601 tccgatggag aagggaacct cccggagaat tcgtaccaga acccttcatg gagattctgg
     3661 gaggatgttg ctacatccta cacagtaaca ttgaccgtga cgaacgcttt tggctctgat
     3721 accattgtca aacaaaatta tatcaggctc ggagacacgc tatcacccgc tcccgttgca
     3781 gcgttcacgt ccagtgtcca gagtggcacc gctcccctga ccgtccagtt taccgaccag
     3841 tcaaccggaa cagcaccgat atcctacgcg tgggacttca acaacgaccg agccatcgac
     3901 agcacagcaa aaaatccggc gttcaccttt gtaactgacg gtgactacac ggtaaacctg
     3961 accgtgacga atgcggatgg aaaagacacc gatattaaaa ccaattatat cagggtcacg
     4021 accccggtca tcacatccgt gccggtagcg caattcactg caaacactac cggaggaacg
     4081 tcccccctga ccatccagtt taccgataaa tctgtttcaa ccggtacgac ttcgtataaa
     4141 tgggatatga ataatgacgg gattgtggat tataccacca aaaacccgtc gcatacctac
     4201 acgacagcag gaagttatac agtaaaactt acggtgacca atgcatccgg aagcgataat
     4261 gagatcaaaa cgaattatat taccctgtcc tctctgcaag ccggagattg ctatggtgca
     4321 gaagcctgca acccgaccgg taatcccctt ggtggcggag caggttatac ccggataatc
     4381 accgaaaaag attcccgggt gaagtatgtc gtatcaacca gggatcagct ggtcactgcg
     4441 ctgaagaatg cgaaatccgg ggaggttgtg ttcgtaaaag gtacggcagt gatcgacatg
     4501 accggaacac cgaataacat catccctgac ggcgtaactc ttgccagtga ccgcggaaac
     4561 ggcggttcga cgggagccct cataaaatac acctcaaata agaatggcag gtatggaacc
     4621 tcgttgttcg ttgccggggg aaataatgtc cgggtaaccg gtttacggct cgaaggtgag
     4681 atgctccccc aggacgggac aggcaatggc gaggccaact atttagttgc catcattgta
     4741 cgcaataagg ccgggctcga agtggataac tgtgagatac gcgggtgggc ttggtcctgc
     4801 gtaaccacca tggacagcac cggcacgtac atccaccaca attacctgca ccacaaccag
     4861 gcccgcggtg aagggtatgg cgctgatatt tacggcggag atatgctggt cgaggccaac
     4921 atcttcaact acaaccggca tgacatagcg gcgggaggat accccgggga aagctatgaa
     4981 gcccggtata accttgttcg cggggacgga catcccattg gcagccacca tttcgatgtg
     5041 cacgcctgca acccgaacaa ctatgccgat gacagtgaga ccagcccggc atttattgca
     5101 gggcatttgt ataagatcca ccacaacacg ttcgaggccg ggcagcaggc ctcgatcggc
     5161 atccgctcgc gcccggtaac gggtgcttat atctacaaca atatcttcat gagtgcctcc
     5221 acggatacag ccggcggtgt cccggtctgg caacgggctg tcacctgggg caacatgttt
     5281 gttaccaata attactggaa gggtaccctc tataccgggg atcagattgt ctggtatctc
     5341 cacccgtgat ccttaaaaaa ctatacaaat cacccttttt tttgcccccc ggcacgtgat
     5401 actttcccgt gaatcgatac gctgtttgtg tgagatcctg atacattgaa tacgaggaga
     5461 aagtgttttt ttcagggcag ctcttatcgc cttgcaattt tttctgaaaa ttgaccatat
     5521 ccggga
//
LOCUS       DASRLV010000137         3504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_47494,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000137 DASRLV010000000
VERSION     DASRLV010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47494"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VFC43_05485"
     CDS             complement(<1..495)
                     /locus_tag="VFC43_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HZK30670.1"
                     /translation="MKILVVGGGGREHAIAAALARNSNTEIFSVMAKQNCGIARIARD
                     VRLCRETDIPRIVSYAKETGVDYAFIGPEAPLEAGIVDQLELLGIGCVGPTQAAARIE
                     TDKAFCRDLMELHNIDGCPKYRVFYDTPEAVSFIKDHEEDLVIKPIGLTGGKGVRIMG
                     EQVDQ"
     gene            complement(530..1042)
                     /gene="pyrE"
                     /locus_tag="VFC43_05490"
     CDS             complement(530..1042)
                     /gene="pyrE"
                     /locus_tag="VFC43_05490"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HZK30671.1"
                     /translation="MVNAIADLLIRYKAIEFGEFTLASGKKSPYYIDVKSAVTNPDLL
                     FAIASTIAQTHTFDVVAGVAVGGVPLAVATAIVTKKPYAIIRSSEKSHGKKEVIIGSV
                     RDTEVLLVEDVTTSGGSALYGINALRTAGAKANRVVTVVDREQGAEEMLRLQGVRLLP
                     LVKVSEILQS"
     gene            complement(1036..1536)
                     /locus_tag="VFC43_05495"
     CDS             complement(1036..1536)
                     /locus_tag="VFC43_05495"
                     /EC_number="2.7.7.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol
                     synthase"
                     /protein_id="HZK30672.1"
                     /translation="MLPAYFPNPIAALCGGGTPVDLGRNFFDGKRVFGNGKTYRGLIT
                     GIAAGIGIGLLQMWLSGIYGWETLPVHTFTSITLLSAGALLGDLIKSFFKRRFGKERG
                     AKWPIADQYDLVAGAFLLLLILDPGWLFANVTIPVFIIILILTPVLHRVVNIIGYHIK
                     VKEVPW"
     gene            1685..3151
                     /locus_tag="VFC43_05500"
     CDS             1685..3151
                     /locus_tag="VFC43_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZK30673.1"
                     /translation="MLIKKTRSLCPICHQAIDAEIAEEEGKIWLDSICHEHGKFHNLY
                     WSDAALYHRFEQYDAVGNGVANPQNVAPPETCPSSCGLCDNHHSQTLLANIDLTNRCN
                     LDCDFCFANARACGFVYEPDFDDIVRMMKVLRDEKPVPAPAVQFSGGEPTMREDLVEI
                     IKKAKEMGFPQVQIASNGVRIAHEPGYAKKLKDTGLNTVYLHFDGVTPATNPLLKIHE
                     QAIENLKAVRLGIVLVPTVIHGKNDHELGDIIRFAIKNISVIRGVNFQPIAFTGAATD
                     DDVRKSRITIPDVLARIEEQMPGILKKDDFYPVPCVLPFSDLVEAYTGKPQVRFTAHQ
                     HCGAATYVFVQSDGSVIPVNRMVDVEQFFETIQHMAEKLKKGGTINKYTALLEGVKNM
                     HESVRNGEQGNTTEFWKIIGKTLIGQNFEALREFHWNALFIGTMHFMDRYNYDLERVQ
                     RCCIHYATPDGKLIPFCTYNSGPVYREKVWKKYAQSKE"
BASE COUNT          903 a          877 c          836 g          888 t
ORIGIN      
        1 ctggtcaacc tgttctccca taatccgcac acctttgccc ccggtcagcc cgatcggttt
       61 tataacgaga tcttcctcgt ggtctttgat aaatgagacc gcttccgggg tatcataaaa
      121 gacgcggtat ttcgggcatc cgtcaatatt atgcagttcc attaaatcgc ggcagaaagc
      181 cttgtccgtc tcgatcctgg ctgcggcctg cgtgggaccg acacagccaa tacccagcag
      241 ttccagctga tcaacaattc ctgcttcgag cggggcttcg ggaccgatga atgcataatc
      301 aaccccggtt tctttggcat aggacacgat ccggggaatg tcagtttccc tgcaaagccg
      361 gacatccctt gctatgcggg caatcccgca attttgtttt gccattacgg aaaaaatttc
      421 agtattgctg ttgcgggcca gagcagctgc tatcgcatgt tctctgcccc ctccacccac
      481 aacgagtatt ttcatgtcta tgtactcttg cgctggcaat aaaaatggtt tacgattgta
      541 ggatctcgct gacttttacc agaggcagga gtcgtactcc ctggagcctg agcatctctt
      601 cagcaccctg ttcccggtcc acaaccgtga caacacgatt ggcctttgca cccgctgtgc
      661 ggagggcatt tatcccgtaa agtgccgacc cgcccgaggt tgttacatcc tccacgagca
      721 ggacctcggt gtccctgaca ctgccgatga tgacttcttt tttaccatgg ctcttttccg
      781 atgaacggat gattgcatag ggtttttttg tgacaatcgc tgttgcaaca gcgagcggga
      841 caccccccac tgcaactcct gcgactacat caaaggtatg tgtttgtgca attgttgaag
      901 caatggcaaa aaggagatcc gggttggtta ccgcggattt tacgtcaatg tagtacgggc
      961 tctttttccc tgaagcaagc gtgaattcgc caaattcaat tgccttatac ctgataagca
     1021 gatctgcgat agcgtttacc atggaacctc cttgactttg atatgatacc cgatgatatt
     1081 taccacccgg tgaaggacag gggtgaggat caggataata atgaataccg ggatcgtcac
     1141 gtttgcaaaa agccaaccgg gatcgaggat gaggaggagg aggaaagctc ctgccacaag
     1201 atcgtactgg tcagcgatag gccattttgc tccccgttct ttgccaaacc tcctcttaaa
     1261 gaaactcttg atcagatccc caagaagggc tccggcagac agcaacgtaa ttgacgtgaa
     1321 ggtatggaca ggcagggtct cccagccata tattcctgac agccacatct gcaacagccc
     1381 tatcccgatt cctgccgcaa ttcctgtgat gagaccacgg tatgtttttc cgttgccaaa
     1441 tacccgtttt ccatcaaaaa aattcctccc caggtcaaca ggagtgcccc ctccacacag
     1501 cgctgcgatc ggattcggaa aataagccgg gagcattatc cagatcgcaa cgagcaacgg
     1561 tattatgaac aactcaatac taataatgta gcactccaat acctgtaata aacagcagat
     1621 acaaacatta aggttactct agcacgagat aaaaagaaca attatggagc gggtggactg
     1681 tataatgctg ataaaaaaaa cccggagtct ttgccccata tgtcatcagg ccattgatgc
     1741 ggagatcgca gaagaagagg gaaagatatg gctggatagc atatgccatg agcatggaaa
     1801 gttccataat ctttactggt cagatgctgc gttgtatcac cgtttcgaac agtatgatgc
     1861 tgttggcaat ggcgttgcaa atccccagaa tgttgcccct cccgagacat gcccgtcatc
     1921 ctgcgggtta tgcgacaacc accactccca gaccctgctt gcaaatattg acctgacgaa
     1981 ccgttgcaat ctggactgtg atttctgttt tgcaaacgcc agggcctgcg gttttgtgta
     2041 tgaaccggat tttgatgata ttgtccggat gatgaaagtt ctccgggatg aaaaacctgt
     2101 tccggctccc gctgtccagt tctcaggcgg cgaaccaaca atgcgtgaag acctggtgga
     2161 aatcattaaa aaagcaaaag agatgggatt tcctcaggtg cagattgctt ccaacggtgt
     2221 caggatcgca catgaacccg gttatgcaaa gaagctcaaa gatactggac tgaataccgt
     2281 ctatctgcat tttgatggag tgacaccggc tacaaatccc ctgttaaaaa ttcatgagca
     2341 ggctatcgag aacctcaaag cagtacgtct tggaattgta cttgtcccga ccgtaatcca
     2401 cggaaaaaat gatcatgaac tcggcgatat tatccggttt gccataaaaa acatctcggt
     2461 aatccgtggt gtcaatttcc agccgatcgc ctttaccggg gcagcgactg atgatgatgt
     2521 caggaaatcg cgtatcacga ttcccgatgt ccttgcacgg attgaagagc agatgccggg
     2581 tattctcaaa aaggatgatt tctatccggt tccctgtgta ctcccattct ctgatcttgt
     2641 tgaagcatat accggtaagc cccaggtcag gttcactgca caccagcatt gtggtgcagc
     2701 cacctatgtg tttgtccaga gcgacgggtc agtcataccg gtaaaccgga tggtggacgt
     2761 ggaacagttc tttgaaacga tccagcacat ggcagaaaaa ctcaaaaaag gtggcacgat
     2821 caataaatac actgcgctgc tcgaaggagt aaaaaacatg catgagtctg tcaggaacgg
     2881 cgagcagggg aacactactg agttctggaa aattatcggt aagaccctca taggacagaa
     2941 ctttgaagca ctccgcgaat ttcactggaa tgctctcttc atcggcacca tgcatttcat
     3001 ggaccggtat aattatgatc tcgagcgggt ccagcggtgc tgcatccatt acgcaactcc
     3061 cgacggaaaa ctcatcccgt tctgtaccta caacagcgga cccgtatatc gcgaaaaggt
     3121 ctggaaaaaa tacgcccagt caaaagagta gcgctggttt ttgtgagtaa tatccggcgc
     3181 tcgcagtaaa cctgaagcct tttttcatct ttattttggt aactttattc taagaagtgg
     3241 ggttttcccg gtttcgctta aaagacctgc tactcagaat tgattttact ggttaatcaa
     3301 gtatttacaa gggtctcccg cctcagggcc tctccgaggc acggcgggac ggaagatttt
     3361 ttagaatttt tcagatattg gtttttcagt cgctgatccg ccgcgggtgc gccccgtcgg
     3421 gggcggcggg gttcatcgtt atagagtttt gagtaggctc ctccttattt ccgggtacta
     3481 taacgtgaat cgtatcttca cgat
//
LOCUS       DASRLV010000138        17002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_615035,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000138 DASRLV010000000
VERSION     DASRLV010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 17002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17002
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615035"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VFC43_05505"
     CDS             <1..774
                     /locus_tag="VFC43_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HZK30674.1"
                     /translation="IKMVGYKPDETLFIPISALGGQNIKVISPEMPWYKGLPLITALD
                     TFKSPDKPTDKPLRLPIQDVYSISGIGTVPVGRVETGIMKKGMKVTFMPANKDGEIKS
                     IEMHHEEIPQAVPGDNVGFNVRGIGKGDIRRGDVCGPADAPPTVADEFTAQIVVLQHP
                     SALTVGYTPVFHCHTTQTACTFVELKKKLDPRSGQTKEENPTFLKTGDAAIVVVKPTK
                     PMVIETVKELPQLGRFAVRDMGSTIAAGMCIAIQAKQMR"
     gene            793..1101
                     /gene="rpsJ"
                     /locus_tag="VFC43_05510"
     CDS             793..1101
                     /gene="rpsJ"
                     /locus_tag="VFC43_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HZK30675.1"
                     /translation="MQKARIRLTGTDFNKVEMVCDRIREIAERTGVNLAGPIPLPTKR
                     LVVPIRKSPDGEGTASWDRWQMRIHKRLIDIDADERALRQLMRIQVPKDIGIEIVLES
                     "
     gene            1177..2739
                     /locus_tag="VFC43_05515"
     CDS             1177..2739
                     /locus_tag="VFC43_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase activity-associated protein Agl23"
                     /protein_id="HZK30676.1"
                     /translation="MIFLLTIILRFWHLDLKLFHHDEAIHSWFSFELLTHGTWVYDPS
                     YHGPFLYYVTAGIFYLFGDSDLTARLLPSLFGTLLIPLVYYIYRIGYLDKKQTLIAAL
                     LVALSPDMVYFSRFLRHDIFMLFFSFLLLVAILYYLEYGKTRFAIIAAVAMAGGLCCK
                     EEMPVIILIFASFFLFAIWQGKFRLPPGWKYDCIMGAFIVFGIMSVLYSAFGFHLETL
                     IGQNFALNTTGWYKAVEHWTAMHNQQRLGGPWFFYIPLFLLYELPIFILGIIGTLQFF
                     FHGFNPSLFIHRLRVRVTYRGSVIPTSELAALSLKQLKNQKPAPAKSDIFFRFCIYWM
                     VLIMAFYAIVGEKVPWLIIPQLLPMCFIAVYKLNWQKTAFALIGCIFLILMTWHVAFV
                     PADINEPIVQVQNSEDLREVMHLIDASDRVVVASQNYWPLPWYYRGDRWKKIKFYGDV
                     TDEATLTRDNPGVIILHDAASYPSIQGYDKKTYKLAYWFSWYDNENRLIDYYLHRDGK
                     MGSINIDVFTPQ"
     gene            complement(3408..4514)
                     /locus_tag="VFC43_05520"
     CDS             complement(3408..4514)
                     /locus_tag="VFC43_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30677.1"
                     /translation="MKGLLKWTVLVLLLICCTHAVSAFSVASVSIDPSGSLTPNNPVT
                     VSFKIEVDDFGSDSEIQLFTDLEKPKWTYTIIVNGVENLRPVTGGRIISISGFELSYK
                     TSDEVAVRVSLEGLAPPVDRTTNKTLIRITEYDGNSKAITSTQVEKTALVINTGDVTS
                     TIQASDAELQDYRTQIDEKAALGIDTSAAEAKYNEANQKISSARSRPSNQYAGALEDL
                     NAAKTAIQDGKTVLDKAWAEYEIAAAQVPINNVDAIIGWFKGNSSTANDQELSTIITK
                     REVAVSYISNANDNIAGGNYVQARQKAQEAFAKGNESYTDALARQKQLMSGIIPSLPK
                     INSTVGIIIGVVVVILIIVGVVIYRKRSQWDELG"
     gene            complement(4524..5702)
                     /locus_tag="VFC43_05525"
     CDS             complement(4524..5702)
                     /locus_tag="VFC43_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin/FtsZ family protein"
                     /protein_id="HZK30678.1"
                     /translation="MRVFFIGFGQAGGKIVDMFIDQDKKLGTNCFRGIAVNTARTDLM
                     GLKNVDMKDRILIGQTMLKGHGVGTDNVTGARVTADEIDSIISAIDIRGTHDIDAFVI
                     VAGLGGGTGSGGSPVLARHLKRIYREPVYALGIIPSPEEGRLYSYNASRSLTTLVNEC
                     DNTFIFDNSAWKNEGESVKSAFQRLNNEVVRRFGVLFRAGEVTKMGVGEMVVDSSEII
                     NTLRGGGITSVGYAISEVMSKQTKKKRGILRNLKDKFGGKQEANEEVLLGEDKSAKVI
                     SLVRRAMLGRLTLPCDYSTAERALVLLAGPPDEMDRKGVEKAKSWVEENIAGVEVRGG
                     DYPVNSEYVAAVVMLATVGNAPRIKELLDIAKETKEDVIKSKEKKSTMFEEGIDPLFE
                     "
     gene            5896..7134
                     /locus_tag="VFC43_05530"
     CDS             5896..7134
                     /locus_tag="VFC43_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZK30679.1"
                     /translation="MKIGIIGGGLTGLVAASRLVQEHEVDLLEKMPYLGGCLSSYHIK
                     DYWIERFYHHCFSGDSGLFALIDELGLSSKLEWKTGTTGYFAKNRIYPLSTPLQILLY
                     PELSLVDKIKLAGLTLKAKKTDLKTLDTIPAEQYITEHLGKNIYSSFFEPLLKSKFGE
                     QRKEVSTAWLFSRIAIRSDRGISGERLGYLNGGFHQLVDALEKSIVSRGGVIRTRTPV
                     SSVSFKEGKWEAGGVRYDAIISTIPPQELEKIGGPAVPRIPYQGAACMTLALERDVTE
                     GIYWLNIKGAAPYGAVIAHTNFIPFTRYGEHIVYLASYFSGTVPQGLDTLMLEDFCNR
                     FSVRMEEIHWHKMAVDPWAGPVYTTGYRDLIPAYEHRGLFMAGMFSRTNYPERSMEGS
                     IRAGSKVAECIMTRSTHGRT"
     gene            7121..7837
                     /locus_tag="VFC43_05535"
     CDS             7121..7837
                     /locus_tag="VFC43_05535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZK30680.1"
                     /translation="MVEPDVTVIIPVFNDLRSLDIAIPRSIEILSRITEGFELIIAED
                     GSTDGSTERVREYEQSDNRIHLLHSDERLGRGKALNRAIYRAKGKIVCYFDVDLATDM
                     QHLPELIDSIRKGADIATGSRLMPESDIIRSEGREIASRSYNFLVRIFLGSKIFDHQC
                     GFKAFNKEHILPILSAIRSDHWFWDTEILVRAQRNGSRVNEFPVRWRAGKGTTVKIKD
                     VFEMGSSILRLWWQIHVSKN"
     gene            7821..8792
                     /locus_tag="VFC43_05540"
     CDS             7821..8792
                     /locus_tag="VFC43_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HZK30681.1"
                     /translation="MYRKISAVVIPTIFAIGIIAYMLFRVWDELFIALENSIPSYLVL
                     GVVICLFAWGLRGWRYRTILKSLDYPMSITASTACIFVSQTVNLIIPARLGDLVRVFI
                     LKHEQNTTYSEGVSSLVVERIFDLFTVALLGAVAVFFVLNVPPWFYTVILVPIIGGLI
                     FFIVLLFLGNFSSENKYVLILLTMLHEVKRASLSVKAILILGWSSIVIWLLDILVCAA
                     VIMMFHQQIPWAIIVLAIVIGNLVKAVPLTPGGIGGYELSLAITFGLVGVDAAIATLI
                     AVIDHLIKNLVTLVGGIVSIYYFGDWVIPSIRSALNTKSDGGKDSDH"
     gene            8767..10791
                     /locus_tag="VFC43_05545"
     CDS             8767..10791
                     /locus_tag="VFC43_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HZK30682.1"
                     /translation="MGGKTLTIEQQIFSVISWLAVITLLQISFYPSLKKTFNSFAFPV
                     SFSSSLLVFTILSWYCGLIRLPVHLALLPFLALFCYHLWYRHYSLDELRTEWRWEMVF
                     LVCFFLMLDVRFVNPTISYAEKFMDHAFLASVMRNPVIPPLDPWFTGGSLNVYYYLGY
                     WMFGCLGIVTGVPSNITFNLALPTVFGLAAINLYAVGSLVIPKFRWLPLLVLFVPNPS
                     FFFQIIQGKPFNTVLWDSTRTISNTINEYPFFSFIWGDVHAHVVSLFNQVFLVFLLLF
                     AYKQWEFLNNRAKWLISILCTLSLGSMPLFNTWDVLIYAPVTLIFVALIIRKSGIKKS
                     TVASWWQVVTIPPLAILCYLPFYLQLKTNTGGISVVHSPSNPFEFLLVNGLFIAIFLV
                     FLWREIVRRPYLLLVLTPFLLTGYIAAAIACIPLMYFIGRKNHSTEDVLAVFGLTLLI
                     LCEIFYLKDSMGDVYYRMNTVFKCYLPAWIMLGTAACLMVGKLLGEADWLPVITARKS
                     GIITTIIVGMLFVLPFVIPFNLNYGTGTLDGMAYLKDTHAGDAGAILYLRTLPVDERI
                     VEAEGGDYTYYSRVSSFTGIPAIIGMPFHEYMWRGDDTGWFNARLADIKSIYEDPEKT
                     LPLMKKYNATLLYVGDAERERYKVNIPVHKLERIYSADGTEIYRFTDYEA"
     gene            10933..11301
                     /gene="rpl7ae"
                     /locus_tag="VFC43_05550"
     CDS             10933..11301
                     /gene="rpl7ae"
                     /locus_tag="VFC43_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7Ae"
                     /protein_id="HZK30683.1"
                     /translation="MAKGYVKTQAPEELQNKALEALEVARDTGKIKKGSNEATKAIER
                     SIAQLVLIGADVEPEEIVMHLGPLCDEKKVPYIFINKQNDIGAASGLDVGSAAAAIVK
                     PGKAKETIDDLTKQLTALKA"
     gene            11312..11521
                     /locus_tag="VFC43_05555"
     CDS             11312..11521
                     /locus_tag="VFC43_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S28e"
                     /protein_id="HZK30684.1"
                     /translation="MADDATPAEVIEVVGSTGMHGEAMQVKCRILDGNNKGRIITRNT
                     VGPIREGDIVMLLETEREAKKMSRR"
     gene            11527..11718
                     /locus_tag="VFC43_05560"
     CDS             11527..11718
                     /locus_tag="VFC43_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24e"
                     /protein_id="HZK30685.1"
                     /translation="MVEQHPCSFCSVQIEPGTGKMYVRKDGTIFYFCSTKCQNNYKLG
                     RVPRKVQWTAAGKKASNKE"
     gene            11727..12179
                     /gene="ndk"
                     /locus_tag="VFC43_05565"
     CDS             11727..12179
                     /gene="ndk"
                     /locus_tag="VFC43_05565"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HZK30686.1"
                     /translation="MDRTFVMVKPDGVQRGLVGGIVARFESKGLKLVAARFESLPEAR
                     VTEQYKEHLSKPFFPSLKKYIMGGPCFLMVWEGRNAVAIARTLIGATNPSEASPGTIR
                     GDFGIDIGRNVIHGSDSAESATREIAIHFRQNDLITYKRVDESVIYEY"
     gene            12254..12904
                     /locus_tag="VFC43_05570"
     CDS             12254..12904
                     /locus_tag="VFC43_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HZK30687.1"
                     /translation="MAGDVLSFALLSLSSIIIIVNPLGATLLYVSLTATLDRPAREIV
                     AKDACRFALLILLFVAVLGAWILQLFGISLEAFRIAGGILLFGIGMEMVYAKTSRTKL
                     TATEKYEFRETDDISIMPLAIPMIAGPGAITTTIVLMNEAIVITPVAIGIVFFSIAIS
                     IIITYYMMRNSDYIMSKIGQREYRAINRLMGMLLIAIAVQFIINGIKMAFPLFTGS"
     gene            12907..13107
                     /locus_tag="VFC43_05575"
     CDS             12907..13107
                     /locus_tag="VFC43_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30688.1"
                     /translation="MEDWDLTTIGLIVIVSSITVAGTIIGAILLGFSVSGAAYLIIIT
                     LVLIVMGAISIFYENTKTSFQK"
     gene            13138..13926
                     /locus_tag="VFC43_05580"
     CDS             13138..13926
                     /locus_tag="VFC43_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HZK30689.1"
                     /translation="MCSAQITSTWEDPEKTLKKAEIFIRHAADCGAKLICFPEQFATG
                     WDPKPHNNIQDIHGSIITTLQNYAKEYRIGIIGSLRERNDPLPKNSAVVINREGRIIA
                     KYAKVHLFSMGQEHKGSAPGTNLGIFTLDSLTCGIAICYDLRFPELFRIYAQKGVHAV
                     FVLSAWPQSRIKHWELFIKARAAENQMYIIGVNTTGKNPVDQYSGASMTADPHGMIIS
                     RANDAEQLLFSDLDPEDVEKLRTDFPIENDRRDKLYHSLLSLTK"
     gene            14043..15251
                     /gene="thrC"
                     /locus_tag="VFC43_05585"
     CDS             14043..15251
                     /gene="thrC"
                     /locus_tag="VFC43_05585"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZK30690.1"
                     /translation="MYHLVCVNCGATYPADEILYNCKKCGHLLAVKYPLDEIKVTKAM
                     WEKRPLSVWRYKELLPVHIDPITLYEGGTPLYHLKKLGEEMGLKHLYAKHEGMNPSGS
                     FKDRGMTVGVSMALQLGKKSVACASTGNTSASLAVYAAKAGIPAVVLLPAGKVAVGKV
                     AQALMHGAKVISIRGNFDRALEMVHDLCISHGLYLLNSINPYRLEGQKTIGFEALDQL
                     GEVPDRFVLPVGNAGNISAVYKGFLELQELGFMDRIPMMTGIQAEGSSPVVRAIRENL
                     PEVIPEDNPETVATAIRIGAPVNAEKALTAIRKTGGLAESVTDAEILHMQKDLARKEG
                     IGVEPASAASVAGIKKLAKLGLIDRNERIVCVVTGHLLKDPDTVIKQCEPPIEINADL
                     PSLLSALHLS"
     gene            15296..15826
                     /locus_tag="VFC43_05590"
     CDS             15296..15826
                     /locus_tag="VFC43_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5803 family protein"
                     /protein_id="HZK30691.1"
                     /translation="MVYPNGTAYRASVEIENTSRYEFVESGLLGEPSPLTVKDIQLSG
                     NCSPCNYSLTGPSFTSNVQAITFEQGNYVVSFTSPIRDNHIQASFKSPYNVNISLPQE
                     FDVRNPLLAGISPGANVTRSPDNTTIIRWNKTQSADLRFYDSGRETLLYFFLQFMGII
                     AIVMLFPFLLTMKKKQ"
     gene            complement(15903..16904)
                     /gene="ruvB"
                     /locus_tag="VFC43_05595"
     CDS             complement(15903..16904)
                     /gene="ruvB"
                     /locus_tag="VFC43_05595"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HZK30692.1"
                     /translation="MTERIISPDPLTEEGEDLTIRPSRLDEFIGQVQVKETLKIAIEA
                     AQMRKEPVDHILFSGPPGLGKTTLAHIIAHELGADIRATTGPVLEKPGDIAALLTPLK
                     RGDILFIDEIHRINPIVEEVLYPAMEDFFIDVMIGEGPSARSIKLNLEQFTLIGATTK
                     QGLLGAPFRDRFGIISRLDLYAPEELMKIVTRSASILKIPITAEGAHEIAQRSRGTPR
                     IVNRLLRRVRDFAIVKGDGTITREITCSALSMLQIDELGLDELDRRILSVIAKDFDGG
                     PVGAKTIAISVGEEVRTIEDVYEPYLIQIGFLKRTPQGREVTPAAKKHLNSSQKTLV"
BASE COUNT         4347 a         4027 c         3936 g         4692 t
ORIGIN      
        1 attaaaatgg ttgggtacaa acctgacgag actcttttca tcccgattag cgctctgggc
       61 ggtcaaaata tcaaggtaat cagcccggaa atgccctggt acaaaggcct cccccttatt
      121 actgctctcg acacattcaa gtcacccgat aaaccgaccg ataaacctct ccggcttccc
      181 atccaggatg tgtacagcat cagcggtatt ggaacagttc ccgttggccg ggtagagacc
      241 ggtatcatga aaaagggaat gaaggtcacc ttcatgcccg ccaacaagga tggagaaatc
      301 aagtccatcg agatgcacca tgaagaaatc ccccaggcag taccgggcga taacgtcggc
      361 ttcaacgttc gcggtatcgg taagggcgat atccgccgcg gcgatgtctg cggaccagcg
      421 gatgcaccac cgaccgtcgc ggatgaattt accgctcaga ttgtggtcct tcagcaccca
      481 agtgcattaa ccgttgggta taccccagtc ttccactgcc atacaacaca gacagcctgt
      541 acgtttgtcg agctcaaaaa gaaactcgat ccacggtcag gccagaccaa agaggaaaat
      601 cccacattcc ttaagactgg tgacgctgca attgtcgtag tcaaaccgac aaaaccaatg
      661 gtcattgaga ctgtcaaaga gcttccccag ctcggaagat ttgcagtcag ggatatgggt
      721 tcaacgattg ccgccggaat gtgcatcgcc attcaggcaa aacaaatgag ataatttttt
      781 ggtggaaata acatgcaaaa agccagaatt cgcctgacag gtactgattt taataaagta
      841 gagatggtct gtgatagaat ccgcgagata gcagaacgta caggtgtaaa tctggccggt
      901 ccgatacccc ttccgacaaa acggctggtt gtaccgatcc gcaaaagccc tgatggagag
      961 ggaacagcct catgggaccg ctggcagatg cgtattcata aacgcctgat agatatcgat
     1021 gcagatgaac gggcattgcg acagctcatg cgcattcagg tgccaaaaga tatcggtatc
     1081 gaaatcgtat tagagagtta aaggtgcggc gtgcagctgg ccgctgaatt ttttaaaaaa
     1141 ataaaacttt ttttttcgtt tgatagaatt tttcttttga tattcttact tacgattatt
     1201 ttgcggttct ggcacctgga ccttaagtta tttcaccatg atgaggctat ccattcctgg
     1261 ttctcctttg aactgctcac acatgggaca tgggtatatg atcccagtta tcatggtcca
     1321 tttctgtact atgttaccgc gggaatattc tatctcttcg gagattcaga tctcacagcc
     1381 cggttactgc cgtcactttt cggaacgctg ctgatccccc ttgtctatta tatttaccgc
     1441 atcgggtacc tcgataaaaa acagaccctt atcgctgccc tcctggttgc gctttctcct
     1501 gacatggtgt atttctcccg gtttctccgt catgacatct ttatgctctt cttctcgttt
     1561 cttttacttg ttgccatcct ttattatctg gaatatggaa aaacgagatt tgctatcatc
     1621 gcagcggtag cgatggcagg aggcctgtgc tgcaaagaag agatgccggt tattattctt
     1681 atttttgcat cttttttcct gtttgcgatc tggcagggga aattcaggct ccctccaggt
     1741 tggaaatatg attgcataat gggtgcattt attgtctttg ggatcatgag tgtcctttat
     1801 tcggcgttcg gctttcatct cgaaacactc attggtcaga attttgcgct gaacacaacc
     1861 ggctggtaca aagccgttga gcactggaca gcaatgcata accagcaacg gttaggagga
     1921 ccatggttct tctacatccc tctctttctt ctctatgaat tgccgatatt tattctcggg
     1981 attatcggga ctctgcagtt cttcttccat ggatttaatc catccctttt catacaccgg
     2041 ttaagagtcc gggtaacata tcgtggttct gtcataccca cgtctgaact tgcagcattg
     2101 agcctgaaac agttaaaaaa tcagaaaccc gcacccgcca aatctgatat ttttttcagg
     2161 ttctgtattt actggatggt cctgatcatg gcattctatg ctattgtcgg tgaaaaagtt
     2221 ccctggctga ttatacccca gctgctgccc atgtgtttta ttgccgtata taaactgaac
     2281 tggcaaaaga cggcttttgc ccttatagga tgcatcttcc tcatcctcat gacctggcat
     2341 gttgcattcg ttcccgccga tatcaatgag ccgatcgtcc aggttcagaa ttctgaggac
     2401 ctgcgtgaag tcatgcatct catcgatgcc tccgaccgtg tggtcgttgc ctcacagaat
     2461 tactggccac tgccctggta ttaccggggc gatcgctgga aaaaaataaa attttatggc
     2521 gatgtaacgg atgaagctac ccttacacgg gataatcccg gagtaataat tctccatgat
     2581 gccgcaagtt atccctctat ccagggatac gacaagaaaa catacaaact ggcttactgg
     2641 ttttcgtggt atgataacga aaaccggttg attgattatt atctccacag agatggtaaa
     2701 atgggtagta tcaatatcga tgtttttact ccccaatgat ttcctgcaga ataaaaaaat
     2761 tgcatactgg ctcatgaaga tatatcgcac ggtttgggtt gatgtctgaa aggattaccg
     2821 cttttcgtga tccgacctcc actaggtata aatctctcta aaaattttct ctggagaaac
     2881 gggcatgaac ctaaaatgtt cacaccggaa ccatgaaaat cgattttctt tgcgatggac
     2941 cccccgcctc agggggcatc tccgaggcac ggcggacaaa aagtattttt aaaatagtca
     3001 tcgatccgcc gcgggggggt cccttcgggg gcggcggcat tcatcatagc tgatcgatag
     3061 aagggttccc attccgaata gcaactgata aacgtaattt tcataggaaa tcatcatgaa
     3121 aattctgacg ctcttttggg cttcgccccc tcgccactgg ggcatcccca ttgtgataat
     3181 aatgttatta cctgttacca aactcgggtc atcgcactgc gccccgtccc ccaacagggg
     3241 attggtggcg caagaggcgg cattatttta ccagaaaaaa ggaattctac agaagcccct
     3301 aaaaaaatgt acttttcagg agtcatcata cccgtaaaga gggaacccat gagcattgtt
     3361 tttcttgttt ttttaaaaaa ggagcagaaa aaaagattat cgaggaatta accgagttca
     3421 tcccattggg aacgcttgcg ataaataacc acaccgacaa ttatcaggat aaccactact
     3481 acgccgataa taataccaac tgtgctgttt atttttggga gggaagggat aattcctgac
     3541 atgagttgtt tttgtcgtgc gagcgcgtcg gtataggatt catttccttt tgcaaaggcc
     3601 tcttgggcct tctgacgggc ctggacataa ttgccgccgg caatattatc atttgcattg
     3661 gaaatataac tcactgctac ttcacgcttt gtgatgatgg tcgagagttc ctggtcgtta
     3721 gccgtgctgg agtttccttt aaaccagcca ataatggcat cgacattgtt gatagggacc
     3781 tgggctgctg caatttcata ttccgcccat gccttgtcca gaacggtttt tccatcctgt
     3841 atggcagttt tcgctgcatt cagatcctcg agcgctccgg catactggtt tgatggccgg
     3901 gagcgggcag agctgatctt ctggtttgct tcattatatt tcgcttctgc agccgaggta
     3961 tctataccaa gtgctgcttt ttcatcaatc tgagtcctgt agtcctgaag ctctgcatca
     4021 cttgcctgaa ttgtagaagt tacatcaccg gtattgatca ccagtgcggt tttttcgacc
     4081 tgtgtgcttg tgatcgcctt gctattccca tcatattctg tgatccggat caatgtcttg
     4141 ttggtagtcc tgtcaacagg tggtgccagt ccttccagag aaactcgaac cgccacttca
     4201 tcggacgtct tgtaactcag ctcaaaacca cttattgaaa tgatacgacc ccccgtcacc
     4261 ggtcgcaggt tttcaacccc attgacgatg atcgtatagg tccattttgg tttctcgaga
     4321 tccgtaaata actgtatctc actgtcagaa ccaaaatcat caacttcaat cttaaatgaa
     4381 accgttaccg gattgtttgg agtcaaggat cctgaaggat cgatagatac cgaggctacg
     4441 gaaaatgcag ataccgcgtg cgtgcagcat atgagtaata gtacaagaac agtccatttc
     4501 aataatccct tcatgataaa acctcactca aataatggat ctatgccttc ttcgaacata
     4561 gttgattttt tctccttgga tttaataaca tcttccttgg tttccttagc aatatcgagg
     4621 agctctttga tcctgggtgc atttcccacg gttgccagca tgaccacagc tgcaacatat
     4681 tcactgttga ccgggtaatc accaccccgt acttcgacac cggcaatgtt ctcttcgacc
     4741 cagctcttcg ctttttcaac accctttcga tccatttcat ccgggggacc tgcaagcagt
     4801 accaatgctc gctctgccgt tgaataatca cagggaaggg tcagccgccc aagcatcgcc
     4861 cttcgcacaa gggatatcac ttttgctgat ttatcctctc caaggaggac ttcttcattt
     4921 gcttcctgtt tgccgccgaa tttatccttc aggtttctga gaatgcctcg tttcttcttt
     4981 gtctgcttgc tcatcacttc gctgatcgca tatccgacag aagtgatacc cccgccacgt
     5041 aaggtgttga taatttcact ggagtcaaca accatctcac caacgcccat tttggtgact
     5101 tcccccgcac ggaaaagtac accaaacctt cggacaactt cattattaag gcgctgaaat
     5161 gcacttttta cgctttctcc ttcgtttttc caggcgctgt tgtcaaaaat aaaagtgttg
     5221 tcacattcat tgacaagagt ggtcagactc cgggatgcgt tgtaagagta caggcgtcct
     5281 tcttctgggg agggaattat ccccaatgcg tataccggtt cgcggtaaat gcgcttgaga
     5341 tgtcgtgcca gcacaggtga accgccggaa cctgtaccgc caccaagacc ggcaacgatc
     5401 acaaacgcgt caatatcgtg cgtgcccctg atatcaatcg cgctgataat gctgtcaatt
     5461 tcatcggcgg ttacgcgggc accggttaca ttgtcagtgc ccacaccatg gcctttgagc
     5521 atggtctgcc cgataaggat ccggtctttc atatcaacat tttttaaacc catgaggtca
     5581 gttcgtgcgg tattaactgc aatccccctg aaacagttgg ttccgagttt tttatcctgg
     5641 tcgataaaca tatcgacaat tttaccgccc gcttgaccaa aacctatgaa aaacacgcgc
     5701 atctagtaga caccatccaa tgggctgaca gataataact ttcttttaat tcttttaagt
     5761 acttttgctg ttataaccgt ttatttcgac aatacaaaaa ctttcttata gtttcttcga
     5821 ggggaaaata ctgatttacg gtaatatacg gagccttttt catttcaccc tgaaaactac
     5881 tatagaatac gtatcatgaa gattgggatt atcggtgggg gtttaaccgg acttgttgct
     5941 gccagcaggc ttgtgcagga gcacgaagtc gatctgcttg agaaaatgcc ttaccttggg
     6001 ggttgccttt catcatatca catcaaagat tactggatag aaaggtttta tcatcattgt
     6061 ttttccggcg attccggtct ttttgccctg attgatgagc tgggattatc ctctaaactt
     6121 gaatggaaaa caggtacgac cggatatttt gccaaaaaca ggatctatcc gctgagcacc
     6181 cccctgcaga tcctgctgta cccggaactc tcgcttgtcg ataagatcaa actcgcagga
     6241 cttaccctta aggcaaaaaa aactgacctg aaaactcttg acaccattcc tgctgagcag
     6301 tacattactg agcatcttgg aaaaaatatt tattcgtcct tttttgaacc tctgttaaaa
     6361 agtaagtttg gggaacaaag aaaggaagta tccacggcat ggttattcag ccggattgca
     6421 attcgatctg atcggggaat ttccggggaa cgcctgggat atttaaacgg gggatttcac
     6481 cagcttgtcg atgctcttga aaaatcaatt gtttcccggg gcggagtaat ccgtacccgc
     6541 acccctgttt catcagtttc tttcaaagag gggaaatggg aagccggcgg ggtccgctat
     6601 gatgcgataa tttctaccat cccgccccag gaacttgaaa aaattggggg acctgcagta
     6661 ccccgaattc cttaccaggg ggctgcatgc atgaccctgg ctcttgaacg ggacgtgact
     6721 gaggggattt actggctgaa tattaagggg gccgctccgt atggtgctgt tattgcccac
     6781 acgaatttta taccattcac cagatacggg gaacatatag tgtacctggc atcctacttc
     6841 agcggaacag ttcctcaagg ccttgacacc cttatgctcg aggacttttg caaccggttt
     6901 tctgtcagga tggaggagat ccactggcat aaaatggcag tcgatccctg ggcgggtccg
     6961 gtatatacaa cgggatatcg tgacctcatt cctgcatatg agcaccgggg attatttatg
     7021 gcagggatgt tttcccggac taattacccg gaaaggagca tggagggatc gatacgtgca
     7081 ggatccaaag ttgcggagtg tatcatgacc aggagtactc atggtcgaac ctgacgtaac
     7141 cgtgattatt cccgtgttta atgacctgcg ctcgcttgat atcgctattc ccaggtctat
     7201 agaaatcctg tcccggataa cggaaggatt tgaactgatt atcgcagaag atggaagcac
     7261 tgatggaagc actgaacgtg tcagggagta cgaacagagt gataaccgca ttcacctgct
     7321 ccatagcgat gaacgtcttg ggcggggaaa agcactcaac cgggcgattt atagggcaaa
     7381 aggaaaaatt gtatgttatt ttgatgttga tcttgcaact gatatgcaac atctccccga
     7441 gttgattgat tccatccgaa aaggagcgga tattgcaacc ggatcacggc ttatgccaga
     7501 gagcgatatt atccggtccg aagggcggga gatagcgagt cgttcatata attttctggt
     7561 caggatattc ctcggcagca aaatcttcga ccatcaatgc ggatttaaag cattcaataa
     7621 ggagcatatc ctcccgatac tgtccgctat acgctcagac cactggttct gggatactga
     7681 gattcttgtc cgcgcacagc gtaatggatc gcgggttaat gaatttcccg ttcgctggcg
     7741 ggcaggaaaa ggtacgacag taaagataaa agatgttttc gagatgggtt catcaatcct
     7801 gaggttgtgg tggcagatcc atgtatcgaa aaattagtgc tgttgtcatt ccaaccattt
     7861 tcgccattgg cattattgca tacatgcttt tcagggtctg ggatgagctg ttcatagctc
     7921 tcgaaaacag cattccttca tatcttgtcc tgggagtagt aatttgtctt tttgcatggg
     7981 gattacgggg ctggaggtac cgaacaatcc tgaaaagtct cgattacccc atgagcatta
     8041 ctgcctctac tgcctgcatt tttgtcagcc agacggtcaa tctgattatt cctgcacgcc
     8101 tcggcgattt agtgcgggtg tttatcctga agcacgaaca aaatacgaca tattctgaag
     8161 gggtatcttc gctcgtcgta gaacggattt ttgatctctt taccgtagca cttctgggtg
     8221 ctgttgcagt tttttttgtt ctgaacgtac cgccatggtt ttatacagtc attcttgtgc
     8281 caattatcgg tggattgatt ttttttattg ttttactctt ccttggaaat ttttcatcag
     8341 aaaacaagta tgtcctgatt ctcctcacca tgcttcacga ggtaaaacga gcttctctct
     8401 cggtcaaagc aatactgatc ctcgggtggt cttcaattgt catctggctg cttgacatac
     8461 tggtgtgtgc agcggtgatt atgatgttcc accagcagat tccctgggca attattgttc
     8521 ttgctattgt catcgggaat cttgtcaaag ctgtgccgct cacgccagga gggatcgggg
     8581 gatacgaact gtcactggca ataacattcg gactcgtcgg agtggatgca gcaattgcaa
     8641 cactgatagc agtaatcgat cacctcatta aaaaccttgt aacgcttgtc gggggaattg
     8701 tatcaatcta ctacttcggc gactgggtta tcccgagtat caggtctgcg ctgaacacga
     8761 aatcagatgg ggggaaagac tctgaccatt gaacagcaga ttttttcagt cataagctgg
     8821 ctcgccgtca ttaccctgct ccagatttcc ttctatccct cgcttaaaaa aacatttaac
     8881 agttttgcct tcccggtttc tttttcttca tcgctccttg tattcaccat cctttcctgg
     8941 tactgcggac ttatccggct tcctgtacac cttgctctcc tgcctttcct ggcacttttt
     9001 tgctaccatc tctggtaccg ccattactcc cttgacgagc tcaggacaga gtggcgctgg
     9061 gagatggtgt tcctggtctg ttttttcctc atgcttgacg tgcgatttgt caatcccacg
     9121 atctcgtatg cggaaaaatt catggaccat gcatttcttg cgtctgtgat gcgaaatccg
     9181 gtcattcctc cgctggatcc atggtttacc ggaggttccc tgaatgtgta ttattatctc
     9241 ggatactgga tgttcgggtg tctgggtatc gtaaccggag tcccctcaaa tatcaccttc
     9301 aacctcgccc tgccaacagt ctttggtctt gccgcgatta acctgtatgc agtgggatca
     9361 ctggtgatcc caaaattccg gtggctaccc ttactcgttc tttttgttcc aaacccttcc
     9421 ttttttttcc agattattca gggaaaacct ttcaataccg ttttatggga tagtacacga
     9481 actatcagca ataccatcaa tgaataccct tttttctcat tcatatgggg agacgtccat
     9541 gcccatgttg tatcactatt taaccaggtg tttctggtat ttctgcttct ttttgcttat
     9601 aaacaatggg aattcctcaa taaccgggca aaatggctga tcagcatctt gtgtacgctc
     9661 agtctggggt ctatgccttt gttcaataca tgggatgttc ttatttacgc acctgtcact
     9721 cttatattcg tggcgctgat tatcaggaag tcagggataa aaaaatccac tgttgcttcc
     9781 tggtggcagg tagtaacaat acctcccctt gcaatcctct gctacctgcc attttatctc
     9841 cagcttaaaa cgaacactgg gggtatatca gtcgttcatt ccccttcgaa tccctttgaa
     9901 ttcctgctgg ttaatggcct gttcatcgcg atctttttgg tctttctctg gcgtgaaata
     9961 gtacgccgtc cctatcttct cctcgttctg actccttttt tacttaccgg ctatattgcg
    10021 gcggcgattg catgtatccc gcttatgtat tttatcgggc gcaaaaatca tagtaccgag
    10081 gatgtgcttg ccgtcttcgg gctgaccctg ttgatcctgt gtgaaatatt ctatctcaaa
    10141 gacagcatgg gagacgttta ctaccggatg aatacggtct tcaagtgcta tcttcctgca
    10201 tggatcatgc tcggcaccgc agcatgtctc atggtgggaa aattactggg cgaggctgac
    10261 tggttaccgg ttatcacagc aaggaaatcg gggattatca caaccatcat cgtcgggatg
    10321 ttattcgttc tcccttttgt tatcccattc aatctgaatt atggtacggg tacactcgat
    10381 ggaatggcgt accttaaaga cacgcatgcc ggggatgccg gagcaatact gtatctcagg
    10441 acattgccgg tcgatgaaag aattgttgag gctgagggcg gcgattatac ctattattcg
    10501 cgggtttctt cgttcaccgg aattcctgca attattggta tgccctttca tgagtacatg
    10561 tggaggggtg atgataccgg ctggtttaac gcgcggctgg ctgatataaa atcaatctat
    10621 gaagatcccg aaaagactct cccgctcatg aaaaaataca atgccacact cctttatgtt
    10681 ggtgatgccg agcgcgagcg ctataaggtg aacattcctg tccataaact tgagagaatt
    10741 tattcagctg atggaacgga gatataccgg tttaccgact acgaggcata agatatgaaa
    10801 aaagtgatgg tttttttgtt cccggcaagt ccttcacaaa cctttatttc tgctcctgat
    10861 gaaatgatat ggctgcatca ttgctgactg atgagtaatg aaggagcatt ctactcctga
    10921 atgaggtgag ttatggcaaa aggttacgta aaaacgcagg ctccggaaga gctacagaac
    10981 aaggcgcttg aagcccttga agttgcacga gatacaggga agatcaagaa aggatccaac
    11041 gaagccacaa aagccattga gaggagtatc gctcaactgg tgctgattgg cgctgacgtt
    11101 gaacctgaag agatcgtgat gcacctggga cctctctgcg atgaaaagaa agttccgtac
    11161 atcttcatca acaagcaaaa cgacattggc gctgcaagtg gtctggacgt cggatcagct
    11221 gctgctgcaa tcgtcaagcc gggaaaggca aaagaaacga ttgatgacct tacaaagcag
    11281 ctgacagcgc tgaaggcgtg aggtttctcc tatggcagac gatgcaacgc cagccgaagt
    11341 gatcgaggtt gttggctcaa ccggtatgca tggcgaggca atgcaggtaa agtgccgcat
    11401 tctcgacggc aataacaagg gacggatcat tacccgtaac actgtcgggc caatccgcga
    11461 aggagatatt gttatgctcc ttgagacaga acgcgaagcg aagaaaatgt cgaggcggtg
    11521 aaaagaatgg ttgaacagca tccctgcagt ttctgcagtg ttcagattga accaggtact
    11581 ggcaaaatgt acgtgcgaaa agatggtacg atcttttatt tctgcagcac caagtgccag
    11641 aataattaca agcttggcag agttcctcga aaagtccagt ggactgcagc gggaaaaaaa
    11701 gcgtcaaata aggagtgaga taattcatgg accgcacttt tgttatggtc aagccagacg
    11761 gggttcagcg gggcctggtt ggcgggatcg tagcccggtt cgaatcaaaa gggttaaaac
    11821 ttgtcgcagc acgtttcgag agcctcccgg aagcgcgggt aacggagcag tataaggaac
    11881 atctctcaaa accatttttt ccatccctta aaaagtatat catgggcgga ccttgttttc
    11941 tcatggtctg ggagggcaga aatgcggttg cgatcgcccg tacgctgatt ggtgcaacca
    12001 atccttcgga agcatcacca ggaacaatcc ggggggattt cgggattgac atcggacgga
    12061 acgttatcca cggttcagac tctgcagaga gcgcaacccg cgagatcgca atccacttca
    12121 ggcaaaatga tcttattacc tataaacggg ttgatgaatc agtcatttac gaatactgac
    12181 aatcttctct ttatttttcc gccgttaaag gctaccaaaa tgtctattac tctttggcac
    12241 tgcactatgc ttaatggcag gtgacgttct tagttttgcg ctgttatcct tatcttccat
    12301 tattattatt gtcaacccgc tgggtgcgac tctcctgtac gtttctctta ctgcaacatt
    12361 ggatcgccct gcccgcgaaa ttgttgcaaa agacgcctgc cgctttgcac tgctgatcct
    12421 gctctttgtt gcggtgcttg gagcatggat cctgcagctc ttcggtatca gtcttgaagc
    12481 cttccgtatt gccgggggta ttttgctttt tggtattggc atggaaatgg tgtatgcaaa
    12541 aacgtcgagg acgaaactga cagcaactga gaaatacgag ttccgggaaa cggatgatat
    12601 ctccattatg ccgcttgcta tccccatgat tgcaggaccc ggggcaatta caacgacgat
    12661 tgtgcttatg aacgaagcga ttgttataac gccggttgcg attgggattg tatttttttc
    12721 cattgcgata tccatcatca taacatatta tatgatgcgg aattcagatt acatcatgag
    12781 caagatcggg cagcgtgaat accgggcgat taaccgtctt atgggtatgc tgctcattgc
    12841 cattgcggtg cagtttatca taaacggtat aaagatggca tttcctttgt tcacagggag
    12901 ttaacaatgg aagactggga tcttacaacc attggattaa tcgtgatcgt aagcagcatt
    12961 acagtcgccg ggacaattat cggagcgatt ctccttggtt tttccgtcag tggtgcagcg
    13021 tatctcatca taataactct ggtactcatt gttatggggg caatttccat tttttatgag
    13081 aatacaaaaa ccagtttcca aaaataacgc ataaaaacgg gggggtattg tatcagaatc
    13141 tgtagtgctc aaattacgag tacctgggaa gatccggaaa aaactctcaa aaaagcagaa
    13201 atattcatcc gccatgctgc agattgtggt gccaagctga tctgttttcc cgaacagttc
    13261 gcaacgggat gggacccgaa accccataat aatattcagg atatccatgg gagcatcata
    13321 acaaccctcc agaactatgc aaaagagtac aggattggta tcattggatc acttcgtgaa
    13381 cgaaatgacc ctctccctaa aaactctgcc gtggttatta acagggaagg aagaatcatt
    13441 gcaaaatatg caaaggttca cctgttctcc atgggtcagg aacacaaagg aagtgcacca
    13501 ggaacgaatc tgggaatatt caccttagat tcgctgacct gtggtattgc aatctgctac
    13561 gacctccgct ttcctgaact attcaggatt tatgcacaaa aaggtgttca tgcagttttt
    13621 gtactatctg cctggcccca gagccggata aaacactggg aactttttat taaggcccgg
    13681 gctgcagaaa accagatgta tattatcggg gtaaatacaa cgggaaaaaa ccctgtcgat
    13741 caatactccg gtgcctcgat gactgcagat ccccacggaa tgataatcag tcgtgcaaat
    13801 gatgcagaac aactcctgtt ctccgatcta gatccagaag acgtggagaa attacggacg
    13861 gatttcccca ttgaaaacga ccgcagggac aaactctatc actctcttct ctcgctgaca
    13921 aaataacccc cggcagcact ttttaaaaaa attttagcac gaggaaaagt atccaaagga
    13981 gcggtctctc cataatatgg taatgcaccc ctatatgtaa ttaaaagacc aatactccaa
    14041 ccatgtatca tctcgtatgt gtcaactgtg gagccacgta tcccgctgac gagatcctgt
    14101 ataattgtaa aaaatgcggt catcttcttg cagtgaagta cccgcttgat gaaataaaag
    14161 taacaaaagc gatgtgggag aagcgaccgc tttccgtatg gcgctacaag gaactgctcc
    14221 ctgtacacat cgatcccata actctgtatg agggcgggac ccccctctac cacttaaaaa
    14281 agctcgggga agagatggga ctcaaacacc tttacgcaaa gcatgaaggt atgaaccctt
    14341 ccggatcttt caaggaccgg ggaatgaccg taggcgtttc gatggcactt caattaggca
    14401 agaagagtgt tgcctgcgca agtaccggaa atacatcagc aagtctggca gtctatgcag
    14461 caaaagcagg gataccagct gttgttctcc tgcctgccgg aaaagtagca gtcggaaaag
    14521 tcgcacaggc gcttatgcat ggcgcaaagg tgatctcgat ccgggggaat tttgatcggg
    14581 cacttgaaat ggtccatgat ctttgcattt cccatggact gtacttgtta aattccatca
    14641 acccttaccg gctggaaggc cagaaaacga ttggttttga agcactcgac cagctgggtg
    14701 aagtgccgga ccggtttgtt ctccctgtcg gaaacgccgg aaatatatcc gcagtataca
    14761 agggattttt ggagctgcag gaactgggat tcatggacag gataccgatg atgaccggta
    14821 tccaggccga gggatccagt ccggtagtac gtgcaatccg cgagaatctt cctgaagtga
    14881 tacccgaaga taatcctgag accgtcgcca cggcaatacg gattggagcc ccggtcaatg
    14941 cagaaaaagc cctgaccgcg atcaggaaga ccggtgggct ggcagaatcg gttactgatg
    15001 cagagatcct gcacatgcaa aaggatctcg cacgaaagga aggtattggt gtcgagccgg
    15061 catcagctgc atcggtggca ggaattaaaa aactcgctaa actgggtctt atcgaccgga
    15121 acgagcggat tgtgtgtgtt gttactggac atttacttaa ggacccggat acggtgataa
    15181 aacaatgcga acccccgatc gagataaacg cagatctgcc ttcactgctg tctgcgctgc
    15241 acttatcata atactgactg ccgtgccggc agtatctgca atctcagctg aatatatggt
    15301 ttaccctaac gggacagcgt accgtgcttc ggtagagatc gaaaatacct ctaggtatga
    15361 gtttgtcgaa tccggtttac tcggggaacc ctcaccactt accgtaaaag acattcagtt
    15421 gtcaggaaat tgttctccct gtaattattc tttgaccggc ccttcattca ccagtaatgt
    15481 tcaggccata acatttgagc aggggaatta tgttgtatcg ttcacctcac ccattcgtga
    15541 taaccatatt caggcttcct ttaaaagtcc ctataacgtc aatatttccc tcccacagga
    15601 atttgacgtg cgaaacccgc tgcttgccgg aatcagccca ggagccaatg tcacccgttc
    15661 ccccgacaat acaaccatta tccgttggaa taagacccag tctgcagatc tcaggttcta
    15721 tgattccggg cgtgagactc tgctgtattt ttttctgcaa tttatgggaa ttatcgccat
    15781 tgtgatgctg tttccattcc tcctgacgat gaagaagaaa caatgagcaa caaaaatagt
    15841 gcacgcatat gaaaaaagta aaaactctca tctctttaat ttttaaatat aactttttta
    15901 catcagacca gcgtcttttg cgaactgttg agatgttttt ttgccgcggg tgtcacctct
    15961 cttccctggg gagtgcgttt gagaaaaccg atctggatca ggtacggctc atacacatct
    16021 tcaatagtcc gaacctcttc tccgacagat attgcaatgg tctttgctcc gaccgggccc
    16081 ccgtcaaaat cttttgcaat aactgaaagt atacgccggt ccaattcgtc aagaccgagt
    16141 tcatcaatct gcaacatgga aagagcgctg caggtgatct cccgggtgat ggtcccatcc
    16201 cctttgacta tggcaaaatc cctgacgcgc cgcagcaggc ggttcacaat ccgtggtgtc
    16261 ccgcggctcc gttgtgcgat ctcatgagca ccttcagctg ttattggaat tttcagaatg
    16321 gaagcgcttc gcgttacgat cttcatcagt tcttcaggag catacagatc gagccgggag
    16381 ataatgccga accggtccct gaacggagct ccaagaaggc cttgttttgt cgttgccccg
    16441 ataagagtga actgttcaag attcagttta atcgaacgtg cacttgggcc ttccccgatc
    16501 atcacatcaa tgaaaaaatc ctccatcgca ggatacagca cttcttcgac aatggggttt
    16561 atccggtgga tttcgtcaat aaacaggata tctccccttt ttaagggagt gaggagcgca
    16621 gcgatatccc cgggtttttc aagaacgggt ccggtggtgg ccctgatgtc tgcaccaagc
    16681 tcatgggcga taatatgtgc cagtgtggtc ttcccaagtc ccggaggacc ggaaaagagg
    16741 atatgatcaa caggttcttt acgcatttgt gcagcttcta tcgcgatctt tagcgtttct
    16801 tttacctgaa cctgtccgat aaattcatcc agacgggaag gcctgatcgt aagatcttct
    16861 ccctcttctg tcagcggatc gggtgagatt atcctttcag tcattgtcat cagtgttcct
    16921 taagtcttga aagagctgcc ttgatgagat cctgaactgt cggagagctg agctgcagag
    16981 atccggcatg tacggcctcc cg
//
LOCUS       DASRLV010000139         5919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1017457,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000139 DASRLV010000000
VERSION     DASRLV010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5919
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017457"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(486..1718)
                     /locus_tag="VFC43_05600"
     CDS             complement(486..1718)
                     /locus_tag="VFC43_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HZK30693.1"
                     /translation="MAVIILAVTFLLIAIRQVGAYNLKIWQIMLGGALAAILTGQISL
                     TGAIHAINIEVMLFLLGMFIVGEALVESGYLSMISCRFFSFARTPGQIIFFILFGMGF
                     LSAILMNDTLAIIGTPLVLGIAAKFRLSLKLVLLSLAIAITTGSVMSPIGNPQNLLVA
                     VNSGLQTPFVTFGLFLLVPTLVSLGLAFLVLRIFFDGEFSNRAIVHEAAPFCDLAKVQ
                     LVKCSLAILLLLSAVNIVASLSGGTLLIPLPFIALCAALPVLIFSSRRLELVRNIDWY
                     TLVFFAAMFVLMKSVWQTGFFQIFVSGPGLMSVPAILGSSVIISQFISNVPFVALFQP
                     MILQAGGTTSMMMALAAGSTIAGNLTILGAASTVIIIQNAEKQGETLTFFEFARVGIP
                     LTVLQIMTYWVWLTVMPF"
     gene            complement(1880..2560)
                     /locus_tag="VFC43_05605"
     CDS             complement(1880..2560)
                     /locus_tag="VFC43_05605"
                     /EC_number="1.1.1.302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone
                     5'-phosphate reductase"
                     /protein_id="HZK30694.1"
                     /translation="MRPHVIVNVAMSADGKISTKERRQVRISGSQDFTRVDRLKAGCD
                     AIMVGIGTILADDPSLTVKSEECREYRRKHGWDDNPVRVVVDSSGRTPQDSSFLNKGQ
                     GKRVVAVSKKADPDTLSLLRKKATVIILGENEVDLFLLLDNLGAMGIRRIMVEGGGTL
                     IAALIRAGLVDEIYTFIGNIIIGGRDAPTFADGEGFILESSFTRLRLLDATRIEEGIL
                     LHWIVPES"
     gene            2902..3972
                     /locus_tag="VFC43_05610"
     CDS             2902..3972
                     /locus_tag="VFC43_05610"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HZK30695.1"
                     /translation="MEFGHLTETSPTSILVVRNYRILYANPAFLFFSGYKDAEIIGQN
                     FLFFIDSSDHQEFTRFSKNWVNETRAPDRGEFQFITKSGLIRIAIVLTTPIIYGDEPA
                     TLVTLVDISEKQRFEDKIRLDNERRRGIIITIAHELRTPLQPILGYLNLLIDDPQGYG
                     ITNETKKILERCLVSVDRERQIINQMLELSVLESGKLRLSCSKFPLSMLLKSVVDTSG
                     YCSKADITIEIPDNLIIIADMDRLFGVFDALLSNAINFSSPPRKVTIMYRSEDHDNCH
                     HIAVKDNGTGISESAFSSIFEPFQLADAAKLSRRYDRIGLSLSIAKKIMLMHGGDITV
                     ESILNSGSTFTLHLPKEGQNSS"
     gene            4106..4834
                     /locus_tag="VFC43_05615"
     CDS             4106..4834
                     /locus_tag="VFC43_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK30696.1"
                     /translation="MLTLRGLTKQIGGLRAVNSVDLSLGANEIVGLVGPNGAGKTTLL
                     NMISGISTPTTGTITFLSEDITGLKADQICKKGIAKTFQIAQSFPGLTARECTMIGAL
                     FGNHRTISMGDAKKEAIEILDFVNFPSEKRETAIKNLNVVELKRVQLARALASKPQLL
                     LLDELTTGLNPRESNEAVDLIKKVRDKGVSILIIEHVMSVIMGVSDRIVVLDHGEKIA
                     DGRPYEVVNDQRVIDTYLGDIDAF"
     gene            4852..5547
                     /locus_tag="VFC43_05620"
     CDS             4852..5547
                     /locus_tag="VFC43_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK30697.1"
                     /translation="MLTVSGLNVFHGNLQVVWDLTFCVKKGELVTMIGPNGAGKTTTV
                     ETIIGLNHKATGSIMFDGENILGSHPYDLFRKGLALVPEKREIFPTMPVIENLYLGGT
                     SNTHKDKTLAHIYRLFPILKERQGQMAGTLSGGEQQMLAIGRALMSEPRLLILDEPST
                     GLSPILTGQVFRSLEQLGREGMTILLLEQNVRNALTMCNRGYVLENGRVVQEGRSKEL
                     LNDPRIQKSYLGF"
     gene            complement(5637..>5919)
                     /locus_tag="VFC43_05625"
     CDS             complement(5637..>5919)
                     /locus_tag="VFC43_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZK30698.1"
                     /translation="LYGVENSFWPVTYVILGGLGTLAGPVIGTVVLTIIWEGLKATGI
                     TFGRYVIVGVILILAIIFLPRGLVSLPEQVQEWLNRRRKKESMPKSPKD"
BASE COUNT         1707 a         1391 c         1374 g         1447 t
ORIGIN      
        1 tcggtaatcg ttgaataatt ccggcctggt tccgcatttt tctaaaatta tgacctctaa
       61 aattcggcgg gtgggggaaa tgaggtataa ccgggctcgt agcaggcccc ggtgtaccag
      121 aattctcaca gggggtttta cggtaaaaat agccccagat gacaataaca tccagcctga
      181 tcttaagtga gattgctcca ataaaacagg atttcccatg aaaatcaaat cattccggaa
      241 tgatgagggg ttaatcattt caattcttca ggtacgatga acgacgacgc ccccgacggg
      301 gcacccccgc ggcggttcag tgacaaaaaa atttaaaact cctcgcccgc cgtgccttgg
      361 agaggccctg atgcggggtc tttcgtatat tgtatgattt tcatggtttt gatgagaacg
      421 tgttaggttc atgctgtttc tacagagtaa aaaaatcact aaaaggagta tctatcaggc
      481 tttttttaaa atggcataac cgtcagccat acccagtatg tcatgatctg gaggacggtc
      541 aatggaatgc ctacccgggc aaattcaaag aaggtgaggg tctcaccctg tttttctgca
      601 ttctggataa tgatgacggt acttgctgca cccagaatag tgaggttacc ggcgatagta
      661 ctgcccgcag caagggccat catcatggat gttgtgccac cagcctgaag gatcataggc
      721 tggaacaggg cgacaaaagg gacattggat atgaactgac tgataattac gcttgaacca
      781 aggatcgcgg gaactgacat gaggcctgga ccggaaacaa aaatctggaa gaatccggtc
      841 tgccagacac tcttcatcag cacgaacatt gcagcgaaaa aaacgagagt ataccagtca
      901 atgttacgta ccagctcgag ccttcgcgac gagaatatca ggacgggcag agcagcgcat
      961 agagcgataa acggaagtgg gataagaaga gtccctcctg acagtgacgc aacaatgttt
     1021 accgcagaca ggagaaggag tattgccagt gaacatttga ccagctggac ttttgccagg
     1081 tcacaaaacg gtgccgcctc atggacgatt gcccggtttg aaaactctcc atcaaagaat
     1141 atacggagga caagaaaagc cagaccgagg ctgacaagag tgggcaccaa aaggaacagg
     1201 ccgaaggtga caaacggtgt ctgtaatccg ctgtttaccg caacaagcag gttctgcggg
     1261 ttcccgatcg ggctcataac actacccgta gtaatcgcta tcgcaagaga gagcaggaca
     1321 agtttaagag agagacggaa ctttgctgcg attcccagaa ccaggggggt cccgatgatc
     1381 gcaagagtat cattcataag gatcgcggag aggaaaccca tcccgaacag tataaaaaat
     1441 attatttgac ccggagtccg ggcaaaggag aaaaagcgac aggagatcat cgagaggtac
     1501 ccgctctcca cgagtgcttc accaactatg aacatcccca gcaaaaaaag cattacttcg
     1561 atattaatcg catgtattgc accagtcaga gagatctgcc cggtgaggat ggcggccaga
     1621 gctccgccga gcataatctg ccagattttg agattatatg ccccgacctg ccggattgca
     1681 ataagcagga atgtgacagc aagaatgata actgcaatat acgtaagatt atgtttttgt
     1741 tcagggagat gagatttggc atttgaacat ccggctcacc aatcataaac agccactgtc
     1801 caggaaatgc cagggaaatg tcgtaccaga aaattcaggc aagaaaatgg aatgttaact
     1861 tgaaaagagg atttcctttt caggattccg gaacaatcca gtgaagaagt ataccttctt
     1921 cgattctggt tgcatccaga agtctgagtc gggtaaatga ggattcaagg atgaatccct
     1981 ccccatcagc aaatgtcggg gcgtctctac ccccgattat gatattacca ataaacgtgt
     2041 aaatctcatc aacgagacct gctctgatga gcgccgcaat cagcgtccct ccgccctcaa
     2101 ccatgatgcg tcggataccc atggctccaa gattatcaag taaaagaaag agatccactt
     2161 cattctcacc aaggataata accgttgctt tttttctcag gagagatagt gtatcgggat
     2221 cagctttttt ggatacggcg actacccttt ttccctgacc tttgttaaga aatgaggagt
     2281 cctgaggtgt acgacctgaa ctatctacaa ctacacggac aggattatca tcccacccat
     2341 gttttctgcg atattcgcga cactcttctg attttaccgt cagcgaagga tcgtcggcga
     2401 ggatagtccc gataccaacc atgatcgcat cacaaccggc ctttaacctg tcaacccgtg
     2461 taaaatcctg cgaacccgat atcctgacct gtctgcgctc ttttgtggaa atctttccat
     2521 cagcactcat tgcaacgttg acgatgacat gcgggcgcat agcgtaccgt ttttacccgg
     2581 cttttatttg aatccactga tctctttttt aaaattatac cagcataaaa ttgtccgtga
     2641 taaaaataag taatatcgta agtacaattg caggtttgaa aaaacctcct tggttttttg
     2701 gaattatttc gttacatgtt gctcaaaatt tacaaataat tcaggaaatt ttaggcgttc
     2761 cagacgtgta tatcttaaaa taacaagagg ttcaaatggc gtagataata aggatgtgaa
     2821 gaccattcag atattccaga atactttgcg acaaaacagg atgacagttg cagagtttac
     2881 ctgtcccgaa gcgcagtgct gatggaattc gggcacctaa cggaaacctc accaaccagc
     2941 atcctggtcg tcaggaatta tagaatactc tatgcaaatc ccgcattttt gtttttttcc
     3001 ggatataaag atgcagaaat tatcggacaa aacttcttgt tttttattga ttcctcagat
     3061 catcaggaat tcaccagatt ttcaaaaaac tgggtgaacg aaacgcgggc accggacagg
     3121 ggtgagttcc agtttatcac aaaatcagga ctgatacgta ttgcaatagt cctcacaaca
     3181 ccaattatat atggtgacga accggcaacc ctggttactc tggtggacat atccgaaaaa
     3241 cagcggttcg aagataagat ccgtcttgac aacgagcgca ggagaggaat aattattacc
     3301 atcgctcacg agttgcgcac ccccctccag ccgattctcg gttatctcaa cctgcttatt
     3361 gatgaccccc agggttatgg tattaccaat gaaacaaaaa aaatacttga acgctgtctt
     3421 gtcagcgttg accgtgaacg ccagataatt aaccagatgc tcgaactatc agtccttgaa
     3481 agtggcaaac tccgcttgag ctgctcgaaa ttcccgcttt caatgcttct gaaaagtgtg
     3541 gtggatacaa gcgggtattg ttcgaaagcc gatataacca tagagattcc tgataatctg
     3601 atcatcatcg ctgatatgga tcggctcttt ggagtgtttg acgcacttct ctcaaacgca
     3661 atcaatttct ccagtccgcc gcgtaaagtt acgattatgt accgctcgga agaccatgat
     3721 aactgccacc atattgctgt caaggataac ggtactggga tctccgaaag tgcattttca
     3781 tcaatttttg agccatttca actcgcagat gcagcaaaac tctcacgtcg ttatgatcgc
     3841 ataggactct cgctttcaat agcaaaaaag atcatgctga tgcacggcgg agatattacg
     3901 gtagagagca tcctgaattc cggcagcaca tttacgcttc acctgccaaa agaagggcag
     3961 aattcttcct gatacttggg agaataaaaa ccgttttttt cgttgtcagg gttgcagaat
     4021 catctttaat caccggatgt ccggcagtat ctttaatacc gcactatgct aacctttagc
     4081 atgttagata tcaacccgtt tccttatgct cacactccgt ggtttaacca aacagatcgg
     4141 cggcctgagg gcagtgaata gtgtagatct ttctttaggc gcaaacgaaa tagtcggtct
     4201 tgtgggaccc aacggcgcgg gaaagactac actcctcaat atgatatccg gtatcagtac
     4261 cccgaccacg ggaacgatta ccttcctctc cgaggatatt accggattga aagctgacca
     4321 gatctgtaaa aaagggatcg caaaaacgtt tcagatcgcc caatcttttc ccggacttac
     4381 cgctcgcgaa tgcacgatga tcggagcatt gtttggtaac caccgtacta tcagcatggg
     4441 ggatgcaaaa aaagaagcga tagagattct tgattttgta aattttccat cagaaaaacg
     4501 ggagactgcc attaaaaatc tgaatgttgt tgaattgaaa agagtgcaac ttgcacgggc
     4561 tcttgcttca aaaccacaac tgctcctttt agatgaactc actaccggtc tcaaccctag
     4621 ggagagcaat gaagcggttg accttatcaa aaaagtgagg gacaaaggag tttccatcct
     4681 gattatagaa cacgtaatga gtgtcatcat gggagtgtcc gaccggatcg tcgtgcttga
     4741 tcatggagag aagatcgcag atggcaggcc gtacgaagtc gtgaatgatc agcgtgtcat
     4801 cgatacgtat ctgggagata ttgacgcatt ttaaaaaaaa ggatcctgtt catgctaacc
     4861 gtttcagggc ttaatgtctt tcatgggaat ctccaggtcg tctgggacct gacgttctgt
     4921 gtcaaaaaag gcgaactcgt caccatgatt ggcccgaacg gtgcaggtaa gaccaccacg
     4981 gttgagacga tcatagggct taaccataaa gcgaccgggt cgatcatgtt tgatggtgaa
     5041 aatatactcg gcagccaccc gtacgatctc ttccgaaaag gactcgccct ggtaccggag
     5101 aaacgggaga tctttcccac gatgccggtg atcgagaacc tctaccttgg cgggaccagc
     5161 aacactcaca aggataaaac tctggcccat atataccgac ttttcccgat actgaaggaa
     5221 cgacaagggc agatggccgg cacgctttcc ggcggtgagc aacagatgct tgccatcgga
     5281 cgtgcgctta tgtcagaacc acgattgctg attctcgacg aaccatccac aggactctcc
     5341 cctattctga ccggccaggt cttccggtcg ctcgaacagc ttggccggga aggaatgaca
     5401 attcttctgc tcgagcagaa tgtccgcaat gcacttacta tgtgtaaccg cgggtacgtc
     5461 ctcgaaaacg gcagggtcgt gcaggaaggt cgttcaaaag agctcctgaa tgatccgcgc
     5521 atccagaaat catatcttgg gttctgaaac ggaacctgct gtacgaaaaa ccggttttac
     5581 aaaggctgtt ccccaaatgt aatttttctt aaaaccaaat atttgcacca gtggtttcaa
     5641 tctttcggag attttggcat ggattctttt ttgcgtcgtc tgttcagcca ttcctgaacc
     5701 tgttccggca ggcttacaag cccccggggc aggaatatga ttgcaagaat gagtatgacc
     5761 ccaacaatga catacctccc aaaagtgatc ccggtcgctt tgagcccttc ccagataatg
     5821 gtgagcacaa ctgtcccgat aaccgggccg gcaagcgtac cgagcccacc caggatgaca
     5881 taggtaaccg gccaaaacga gttttcaact ccgtaaagc
//
LOCUS       DASRLV010000140         5848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1180231,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000140 DASRLV010000000
VERSION     DASRLV010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180231"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VFC43_05630"
     CDS             <1..180
                     /locus_tag="VFC43_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30699.1"
                     /translation="VKLWCALLNDLHQMSQQNKKIAPHLMGIYHNLHQWDDFLEDMKI
                     FRIKGPCGIKSGSVL"
     gene            complement(252..476)
                     /locus_tag="VFC43_05635"
     CDS             complement(252..476)
                     /locus_tag="VFC43_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZK30700.1"
                     /translation="MKNRIKVYRAMRNLTQEALADQLGVTRQTILAIEKGKYDPSLEL
                     AFRISKMFRVSIDKMFFFESETEDQPVFED"
     gene            complement(547..852)
                     /locus_tag="VFC43_05640"
     CDS             complement(547..852)
                     /locus_tag="VFC43_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30701.1"
                     /translation="MEYRKLNASGSATTWQDAHTLVILTAGACLLALIISLKSILNVP
                     VERLTLELILCTSILGGLVTSYPLVTRKRSDLAISHPGIWILLIVVITMSIIVINAV"
     gene            1073..2995
                     /locus_tag="VFC43_05645"
     CDS             1073..2995
                     /locus_tag="VFC43_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HZK30702.1"
                     /translation="MEHKSVKVGIIQMAVGPDPNENIKKALLKVEQAALQGAQIICLP
                     ELYRTRYFPQGIGIGAEALAETIPGDSTRVFSEIARRNNVVIIVPIFEKSYEGIYYNT
                     AVIIDADGTLHTPYHKVHIPQDPGFFEKGYFYPGNRYYVHTTRYGRIAVLICYDQWFP
                     EAARCVALEGAQIIFYPTAIGHPTSEEPAEGNWREAWALIQRGHAVANSVHVAAINRV
                     GKEADINFFGGSFICDAFGKIILQAGDMEDTLIASLDLSMNETVRESWGFFRNRRPDT
                     YGATCTLFPGNDGGFPEIRQGDTPRNRGFHMPAEWEPHEAVWLSWPHNRNTFPNLSLV
                     EDAYYAFINAIHASERVELFIPTAALHRKIRANLREAHIDLSRVTLHTIEFSDVWIRD
                     YGPTFVVNRAMNRAAMVRWNFNAWGLKYEDQVTDGKIPAIMNRWLDLPQFVPGIVLEG
                     GSFDVNGRGTLLTTRSCLLNPNRNPSLSEDQIETFLKEYLGVVKIIWLNDGIVGDDTD
                     GHIDDIARFVTPSTVVCAYEADTEDANFRALQENYEILKRSSNQDGVPLTVVKLPMPA
                     KVATGEERCPASYTNFYIGNTVVIVPVFNDPNDAEAIRIIQDLFPGRKVIGINARAMV
                     EGFGTFHCATQQQPRI"
     gene            3091..3582
                     /locus_tag="VFC43_05650"
     CDS             3091..3582
                     /locus_tag="VFC43_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HZK30703.1"
                     /translation="MNQPPIVRPGDPAKNFSLKDQNDTTFDLYENMENWTLLSFHPLA
                     WTAFCAGQMQSLEDNREVLSALSTSAVGISVDAVPCKKAWAESLGITHTPLLCDFWPH
                     GAVAHQYGLFREANGFSERANVIIDENQNIVFVKVYPVHTVPDIGEVISFIKTEKDCE
                     KTE"
     gene            4075..4479
                     /locus_tag="VFC43_05655"
     CDS             4075..4479
                     /locus_tag="VFC43_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30704.1"
                     /translation="MQETCSSMINPKQEEKLKIAVENTCELCHEYHPSASLEIHLISR
                     RCYKEMKRDQSTRILIVCHPCHIHIHRLPLPVGRQRMIVKGRSFFIRRDLRQVLGYIP
                     KPYQAPDDINLYVIYEEYMGRSSPGSYRLERH"
     gene            4650..5342
                     /locus_tag="VFC43_05660"
     CDS             4650..5342
                     /locus_tag="VFC43_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZK30705.1"
                     /translation="MNSRALTVILIAAVIVAAAVFFLLGGNNRISNPGGGPNVLPGVE
                     VRSYQGKDLSSINDFRENSIKGPQFVNTSGYRLTISGLTNRTSEYTYQDILNKYPHNS
                     KVVTLFCVEGWDTTILWEGVLVRDLIRDAGIDPRANTVIFTAHDGYTTSFPLEYLMSR
                     DIIMAYKMNNVTLPAERGYPFQLVAEEKWGYKWIKWIEKIQLSDDPKYRGFWEQRGYS
                     NTGDLSNNFFGY"
     gene            5598..>5848
                     /locus_tag="VFC43_05665"
     CDS             5598..>5848
                     /locus_tag="VFC43_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30706.1"
                     /translation="MDQGNLVAREHDHDVLRHEPRRNPQGIFLNQDRDSPKLPLRKNC
                     SRVEIPSQPTLYHKDSLNIMKWYDILNLFPNTAGNYYHIP"
BASE COUNT         1640 a         1330 c         1335 g         1543 t
ORIGIN      
        1 gttaagttgt ggtgtgcact attaaacgat ttgcatcaaa tgtcacagca gaataaaaaa
       61 attgcaccac atttaatggg tatctaccac aacttgcacc aatgggatga ctttcttgaa
      121 gatatgaaaa tttttcgcat caaaggtcca tgcgggataa aatccggttc ggttttatga
      181 ggagaagttt actggctctt accaagggga ttttttaaaa aaaagccaac atgacctgca
      241 ggagtcctga tttaatcctc aaagacgggc tgatcttcag tttcggattc aaaaaaaaac
      301 attttatcga ttgaaaccct gaacatcttt gagatccgga aggcgagttc aagcgaggga
      361 tcatactttc ctttttctat agctaaaatt gtctgccgcg ttacaccaag ttggtcagca
      421 agcgcttcct gggtgaggtt ccgcattgcc cggtagactt taatcctgtt tttcattgtt
      481 cagatatccg gatttttatt tttggaacat tcaaacagca tcgcctctgc aggaatcaaa
      541 tggcattcag accgcgttta tgacgatgat tgacatagtg atgacgacga tcaacagaat
      601 ccagataccg ggatggctga ttgccagatc cgaccgtttt cttgttacca ggggataaga
      661 agttacaaga ccccccaata tactggtaca aaggatcaac tcaagtgtga gccgttcaac
      721 gggaacgttg agtatacttt ttagagatat aatgagagca agcaggcatg cacctgccgt
      781 aagaattacc agcgtgtgag catcctgcca tgtggtggct gacccggaag cgttcagctt
      841 cctatattcc attcgatcca tatatgtcca acatccataa cttaatgtaa attataatta
      901 acattttaga ataaatactt tactttttgt caaataaatt tcccactgga aactattttc
      961 cgcaccacat ttccattcac gcctttctga tccttatctg gcatacgtac caatcatgcc
     1021 gtttggatag catgataacc aagtaagaaa aaactcgtat agaaagaggc atatggagca
     1081 taaaagcgta aaagtcggta ttatccagat ggcggtaggt ccggatccta atgaaaatat
     1141 taaaaaggca ttattgaaag tcgaacaggc tgcactacag ggagcacaga taatatgtct
     1201 gccagaactt taccgtacac gttacttccc ccaggggata ggtataggag cagaagccct
     1261 tgcagaaacg attccgggag attcaacacg ggtgttttct gagattgccc gcaggaacaa
     1321 tgttgttatt attgttccga tttttgagaa atcttatgag ggcatatact ataacacagc
     1381 cgtaattatc gatgcagacg ggacactcca cacaccctat cataaggttc acatacctca
     1441 ggatccgggt tttttcgaaa aaggatattt ttatcccggg aaccggtatt atgttcacac
     1501 aacccggtac gggcgaattg ctgtgctgat ttgctatgac cagtggtttc ccgaagctgc
     1561 gcggtgtgtc gcgcttgagg gagcccaaat cattttttac cctacggcaa tcggacatcc
     1621 cacgagtgag gaacctgctg aaggaaactg gcgtgaagca tgggcactca tccagcgagg
     1681 gcatgccgtt gcaaacagcg tccatgttgc tgcaataaac cgggtcggta aagaggcaga
     1741 tataaatttt tttgggggtt cgttcatctg tgatgcgttt ggtaaaataa tcctgcaggc
     1801 aggagacatg gaggatacac tgatcgcctc tcttgattta tccatgaacg aaacggtcag
     1861 ggaatcctgg ggatttttcc ggaaccgccg ccctgacacg tatggggcga cctgtactct
     1921 gtttccgggt aatgacgggg gatttccgga aatacgtcag ggagatactc ccagaaaccg
     1981 tggattccat atgccagctg agtgggaacc tcacgaagca gtgtggctgt catggccgca
     2041 taacaggaat acattcccca atctctccct tgttgaagat gcatattacg catttatcaa
     2101 tgcaattcac gcctcagaac gggtagagtt gtttatcccg actgctgctc tccatagaaa
     2161 aatccgcgca aacctccgtg aagcccatat agacctttcc cgggtaactc tccataccat
     2221 cgagttttct gatgtctgga tccgtgatta tggtcccaca tttgtggtaa accgtgcaat
     2281 gaaccgggct gcgatggtgc gatggaattt caatgcctgg ggtctgaaat atgaagacca
     2341 ggtcacagac ggtaaaatcc cggctataat gaaccgctgg ctggatctgc cccagtttgt
     2401 tccgggaatc gtactcgaag ggggatcttt tgatgtcaat ggcaggggga ctctcctgac
     2461 tacccgctca tgtcttttaa acccgaaccg caatccttca ctctcggaag accagatcga
     2521 aacgttcctg aaagaatacc tcggggttgt gaagattatc tggctgaacg atggtattgt
     2581 cggtgatgat accgatggtc atatcgatga tatcgcccgt tttgttaccc catcaacggt
     2641 cgtctgcgcg tatgaagccg atacggaaga tgcgaatttc cgcgccctgc aggaaaatta
     2701 cgaaatcctg aaacgatcca gcaaccagga cggggtacca cttaccgtgg tgaaactccc
     2761 gatgccggca aaagttgcaa caggtgaaga acgttgcccg gcgagttaca caaattttta
     2821 tattggaaat accgtcgtta ttgtgccggt tttcaatgac ccgaatgatg cagaagcgat
     2881 acgcattatt caggatcttt tcccgggacg caaggtaatc gggattaatg cccgggcaat
     2941 ggttgaggga ttcggtacat tccactgtgc tacgcagcag cagccccgga tataagagcc
     3001 tggaattccg tcatctggcg ctctggcagg tcgctattac aaagcctgat atctgttgaa
     3061 gaagaggaaa aatacaaaaa ggatgaacgt atgaaccagc cacccatcgt cagaccggga
     3121 gatcctgcaa agaatttctc cttaaaggac cagaatgaca ccacattcga cctgtatgaa
     3181 aatatggaaa actggacgct attgtcgttt catccccttg catggacagc tttttgtgcc
     3241 ggccagatgc aatcgcttga agataaccgg gaagttctat cggcactgag taccagtgct
     3301 gttggaatca gtgtggatgc agttccctgt aaaaaagcct gggcagagtc tcttgggatt
     3361 acccataccc ccctgttatg tgatttctgg ccgcatgggg ctgtggctca tcagtatggg
     3421 cttttccgtg aggcaaatgg tttttcagaa cgagcaaacg tcattattga cgaaaaccag
     3481 aatattgtgt ttgtaaaggt ttatccggtc cataccgttc cggatattgg cgaagtgatc
     3541 tcgtttatta aaacagaaaa agactgtgaa aaaacggagt agaaacagac atgaaccgag
     3601 cgttcatacc caaatcatga aaatcggttt tctctacgat ggacccccgc ctcagggcct
     3661 ctccgaggca cggtgggcag ggagtttttt agtcattaat ccgccgcggg ggcgtccctt
     3721 tttggtgcga cggcattaat cataattgat cgatagaata atcccaattc caaaatcgca
     3781 tctgataaaa aatgatataa tatgaaaata ccatcacttt ttcttccgta tgcttgtggc
     3841 cctccggcat aatgttcgtt ttcataggaa acctcgttta atagaaagcg tgctaggatc
     3901 aaagactttt aaccacctta cttccatctc tttttgtaaa ggatatcttc gttttattct
     3961 gtaacaaagc ttcgagacat gctgagcaga ctgttattct ggaaagtgat ggcacatggg
     4021 gaaatctttt attccgggtc agacaatggt cgggtgataa taaggtgaag gattatgcag
     4081 gaaacctgca gttctatgat aaatccaaag caggaagaaa aattaaaaat tgccgtcgaa
     4141 aacacatgtg agctctgcca cgaatatcat ccatcagcat cccttgaaat tcatctcatt
     4201 tcccgccgct gttacaaaga gatgaagcgg gatcagtcga ccagaatact catcgtctgc
     4261 catccctgcc atatccatat ccaccgtcta cccttacccg tcggaagaca acgaatgata
     4321 gtcaagggac gttccttttt cattcggagg gatttgcgtc aggtcctggg gtatatacca
     4381 aaaccctatc aggctccgga tgatatcaac ctctacgtta tctacgaaga gtatatgggc
     4441 agaagttccc cgggatcata ccgcctggaa cggcattgac ctggtcacgg accgccaact
     4501 cccgtacatt gaccggctgg atatttttga aaaatccttt cgtgcaagta aggtagcact
     4561 ttttggtaac ggcagaggta aagcctccct ttaacaaaag ggtgtcccta tacgatcatt
     4621 attaagatac ggggtatagt tcttttttaa tgaattcaag agctcttact gtcatactaa
     4681 tcgccgctgt gattgttgct gcagctgttt ttttcctgct gggtggaaat aacagaatca
     4741 gcaatcccgg cggaggtcca aacgtgcttc ccggagttga agtcaggtcg taccagggaa
     4801 aagatctctc ttcgatcaat gattttcgtg aaaattcaat caaaggtccg caatttgtaa
     4861 ataccagcgg ttaccggctt accatttcag gactgaccaa tagaacatct gaatatacct
     4921 accaggacat tctgaacaag tacccccata actcaaaggt agtaacactt ttctgcgtgg
     4981 aaggatggga taccacaatc ctctgggagg gtgtgctggt gcgtgatctg atccgggatg
     5041 ccgggataga tccccgtgca aataccgtta ttttcacggc ccatgatgga tatacgacat
     5101 cattccctct ggagtacctg atgagccggg atattataat ggcctataaa atgaacaatg
     5161 tcacgcttcc ggccgaacgg ggatatccat tccagcttgt tgctgaagaa aaatggggat
     5221 ataagtggat caaatggatt gagaaaatcc agttgtctga tgaccctaaa tatcgtggat
     5281 tctgggaaca gcggggatat tcaaataccg gagatttaag caataatttc tttggatatt
     5341 aacaagccgg aaaatttccc ttttttaaaa gcatacccac aagatcgccg ctattacgtg
     5401 aataaaaatt tttcagagac aaaacactcc ccatgcctca cgcatatagt aataaaatta
     5461 tggatttccc gacgttaaac cgatgagaca tgtatcgtat gaacactatt cctggctctt
     5521 gtccgggaga aaacctatcg ccttttcaag agtcagtttc acgctctccg ggtcatgtac
     5581 tccaaacaat gagatatatg gatcaaggga acctggttgc cagggaacat gaccacgatg
     5641 tgctccggca tgagccccgc cgcaacccgc agggtatttt cttaaatcaa gatcgcgatt
     5701 ccccaaaact acccttgagg aagaactgtt cccgtgttga aataccgtcc cagccgaccc
     5761 tgtaccacaa ggattctctc aacattatga agtggtatga cattctcaac ctgtttccca
     5821 ataccgccgg taattattac cacatccc
//
LOCUS       DASRLV010000141         5835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_49490,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000141 DASRLV010000000
VERSION     DASRLV010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49490"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="VFC43_05670"
     CDS             <1..713
                     /locus_tag="VFC43_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZK30707.1"
                     /translation="DPDLHPAEIKQRLGVVPQETNLDPDFTCFGNLFSYARYFDIPPD
                     SAKKKANDLLAFVQLQEKRDVTVEKLSGGMKRRLILARALVNNPDLLILDEPTIGLDP
                     QARHLIWERLKLLQSQGNTIVLTTHYLDEAARLCDRLVIMDNGKILVEGSPEDLVKQY
                     VGSEIVEVEKTDDVVSCLADRNIPFEVIGDMVQIASDSSREVARILFEHCSPHKVLTR
                     SATLEDVFLKLTGRKLRE"
     gene            716..1501
                     /locus_tag="VFC43_05675"
     CDS             716..1501
                     /locus_tag="VFC43_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK30708.1"
                     /translation="MQPVWTIPTITRRAWKVWYRNLVVFFRTWHVNFFPPLIEAFLYL
                     FAIGLGIGTYVKEIDGIPYVNFIAPAILAISVMNSAFFECTYGSYVRMYYQKSFDAMI
                     ATPLSIEDVIAGELLWGATRSVLYVTIMLPVLVAFGVISLPLSLLAIPLAFVGGLMFA
                     GLAMCFTAVTPGIDTLNYPSFLLITPMALFSGTFFPLTLLPEALHYIALALLPLTHLV
                     ALMRMITLPALTGLAVLHLTWIIMVTILFCIIAINLMKRRLIV"
     gene            complement(1572..2126)
                     /locus_tag="VFC43_05680"
     CDS             complement(1572..2126)
                     /locus_tag="VFC43_05680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK2/MTQ2 family protein methyltransferase"
                     /protein_id="HZK30709.1"
                     /translation="MPFDASQVYVPDADTYLLLDAARLEVVAGDRVLEVGTGCGLIAA
                     ELARIAGVVATDINPHAALCAKKRGLEVIQGDLFCGIRSTFDLVLFNPPYLPTKPEER
                     IDDWLEHALDGGETGRVVIERFAEQVGRVLAPEGRILLLISSLTGLPEVRDLFFGLGF
                     ISEIMVRKKIEGEMLYVLKIVRQK"
     gene            complement(2594..3361)
                     /gene="rsmA"
                     /locus_tag="VFC43_05685"
     CDS             complement(2594..3361)
                     /gene="rsmA"
                     /locus_tag="VFC43_05685"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HZK30710.1"
                     /translation="MRAHHDQHFLTDPHAVDRIADLTELKGKRVLEVGPGNGALTRAL
                     LDRGAIVHAVELDGNLCEELADRFSTEISAGQLIVQHGDATRCELPFFEIVVSNLPYS
                     VSSKITFRLLEVGFKEAVLMYQSEFADRMLAPAGTKDCGRLSIMVQTYATVQRCFKLP
                     PNCFTPRPQVHSTVVKIIPREHPIFPISDRHRYADVVRALFSHRRKTIRNCLKGSGGM
                     LDPEWVKRVIAVLPDEILKSRPEELYLEDFATIANME"
     gene            complement(3358..3921)
                     /locus_tag="VFC43_05690"
     CDS             complement(3358..3921)
                     /locus_tag="VFC43_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF655 domain-containing protein"
                     /protein_id="HZK30711.1"
                     /translation="MKAEKKEVNAIVLDVLLKGHPEDPRPPFKREPIIQAMGLEQFKL
                     LELVPKNNVLIQLYDKVYIGDAERDKIDRVKRRIGYNDLTQTAKGELPFIIEKVIKDR
                     EPDFVAFFNKAISITPKLHMLHLLPGIGKKLMWEILEERNKKPFASLADISARIKSIP
                     HPERMIQSRILEELQDPDVKYHVFTTR"
     gene            complement(3933..4283)
                     /locus_tag="VFC43_05695"
     CDS             complement(3933..4283)
                     /locus_tag="VFC43_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase Rpb4 family protein"
                     /protein_id="HZK30712.1"
                     /translation="MKVKSIISEERVTLPEVRGVLQGVESMRIAAEKEMSYEFRRSIE
                     HANKLAKTTPEKAHELVADLLKMEKMKPEIAYRIANIMPKTRDEVRAIFAKERYTLSP
                     EELDKIIEHVMAHF"
     gene            complement(4305..4595)
                     /locus_tag="VFC43_05700"
     CDS             complement(4305..4595)
                     /locus_tag="VFC43_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21e"
                     /protein_id="HZK30713.1"
                     /translation="MAHHNGPRKKTRYKFKKDLRERGLPPVTCVIRKYEIGDRVHIVC
                     NPSIQKGMPHRRFHGLTGTILGQRGRAWMLTIRDGDADKIVIARPQHLKAQK"
     gene            complement(4599..5831)
                     /locus_tag="VFC43_05705"
     CDS             complement(4599..5831)
                     /locus_tag="VFC43_05705"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(54/55) synthase Pus10"
                     /protein_id="HZK30714.1"
                     /translation="MELDEQVTSILAYGECCDHCLGRFFGKRSHGLSNDERGRGLKIA
                     RALSENQPYTKFSGTCWVCSNFFATIPEWADRVVQAVRGIEYSTFLIGCRVPPLIAEN
                     EEMVWSDLSLTEPEPFKSEVNREVGKAVSVLTGKNVDFKKPDIVAILDPASGRVEVQI
                     NSVFFYGRYQKFERGIPQTHWDCRACRGAGCEKCNFTGAQYLDSVEELIARPVIIAFE
                     AQNAVLHGAGREDIDARMIGSGRPFILEVVEPKKRSLDLAWLEQEINRTAVGRVSVTI
                     KRWSERAEVETLKSNKAHKKYRILVGVDGALSADEFANALKTLQGATIHQRTPERVAH
                     RRADKIRERIVLDIEYAGEQDGKFVVEILGEAGLYIKELVSGDGGRTRPSLAEILDRP
                     AHVISLDVVQVEGVKEGD"
BASE COUNT         1333 a         1612 c         1386 g         1504 t
ORIGIN      
        1 tggacccgga tctccacccg gccgaaatca aacaacggct cggggtagtc ccgcaggaga
       61 caaatctcga tccggacttt acctgttttg gcaacctctt ttcctatgcc cggtatttcg
      121 atattccccc cgatagtgcg aaaaagaaag caaacgacct tctcgcgttt gtccagctgc
      181 aggaaaaacg tgatgtcacc gtagagaaac tctccggggg gatgaagcga cggcttattc
      241 ttgcacgcgc tctggtcaac aatcccgacc tgctcattct tgacgaaccg acgattgggc
      301 ttgaccccca ggcacgtcac ctgatctggg aacgactgaa actgttacag tcgcagggta
      361 atacgatcgt acttaccacg cactacctgg acgaagcagc ccggctctgt gaccggctcg
      421 tcatcatgga caatggaaaa atccttgttg aaggctcacc ggaggatctt gtaaaacagt
      481 atgtcgggag cgagattgtt gaagtcgaaa aaactgacga tgttgtatcc tgtctcgcag
      541 acaggaacat tccctttgaa gtgatcgggg atatggtaca gatagcatcg gactcatccc
      601 gtgaggtcgc ccgtatcctg ttcgaacact gctcccccca caaggtgctg acccgatctg
      661 caacccttga agacgtgttc ctgaaactga ccgggaggaa actcagggag taatcatgca
      721 gcctgtctgg acaatcccca caatcacgcg gcgggcgtgg aaggtctggt accgcaacct
      781 cgtagtcttt ttcaggacct ggcacgtgaa ctttttccct cccctgattg aggcattcct
      841 gtacctgttt gccattggcc ttgggatcgg tacctacgta aaagagatcg acggaatccc
      901 gtatgtcaac tttatcgcgc cggcaatcct tgccatctca gttatgaact cagcgttctt
      961 cgagtgcacg tacgggtcct atgtcaggat gtattaccag aagagttttg atgcaatgat
     1021 cgcaaccccc ctctcgattg aagatgtgat tgccggagag ctcctctggg gggcgacccg
     1081 gagcgtgtta tacgtaacca tcatgcttcc tgtgcttgtc gcgttcgggg ttatctccct
     1141 tcctctctca ctgctggcca tcccgctcgc gtttgtcggc ggtcttatgt ttgccggtct
     1201 ggcgatgtgc tttactgctg tcacgcccgg catcgatacc ctgaactatc cctcgttcct
     1261 tctcatcaca ccgatggcac tcttctccgg cacgttcttc ccgctcaccc tgctgcccga
     1321 agccctgcac tatatcgcgc ttgccctgct cccccttacg catctggtcg ccctgatgag
     1381 gatgatcacc cttccggccc ttaccggtct ggccgtgctc catctcacgt ggattatcat
     1441 ggtgacgata cttttctgta tcattgccat caacctgatg aaaagacgcc ttattgtata
     1501 aaaatgaggt ttgaatcagc attgtatccg caaaaatagc ggttttttag catactatat
     1561 ctttggcacc gttatttctg ccggacgatt ttcagcacgt acagcatctc accttcaatt
     1621 tttttcctca ccataatttc cgatatgaaa cccaacccga aaaaaagatc ccgcacttcc
     1681 ggcagcccgg tcaaagaaga gatcaacaac aaaatccttc cttccggtgc caggacccgt
     1741 ccgacctgtt cagcaaaacg ctctataacg acccgaccgg tctccccgcc atcgagtgca
     1801 tgctcaagcc agtcatcaat cctctcttcc ggtttcgtcg ggagataggg ggggttgaac
     1861 aggaccaggt caaatgtgct tcggatccca caaaaaagat cgccctggat tacctctaat
     1921 cctctttttt tggcacaaag cgccgcatgc gggttgatat cagtcgcgac tacgcccgca
     1981 atacgtgcaa gttcggcggc aatcagcccg cacccggtcc ccacttccag gacacggtcg
     2041 ccagctacaa cctccagcct tgctgcatcc agcaggagat aggtgtcggc gtccggcacg
     2101 tagacctgac tggcatcaaa cggcatacat caacccatca cgatagcctg ccatgccggt
     2161 catccttcac attctcccat gtacgcatgc tgttttaccg gagatgataa aaaataaccg
     2221 cccgacaatc tccccaaaag agaaacggga acgaaaaatc caatgatgca ttcatttcca
     2281 gcctgttcac cctggttgta tcaaaaagat tccttgtgtg taagcagttc acctggacag
     2341 atcatggagc agaaggttct gatgcatgag cggttggttg atccaaagca cattttgcaa
     2401 aatgagtcta agtatacata aactcaccgg cagtttaaat caagatttcc agggaatttt
     2461 tcatgaaagc atgtcctttc tcaccaacaa aaaaggatac ggtggtgaat gataataccg
     2521 ggtaggcata acagccacgc tgccagcagg ggaaataaaa aagaatcgta ccgggtacta
     2581 gcgttcgggc tgtttattcc atattcgcga tcgtggcaaa atcttcgaga tacaattcct
     2641 caggcctgct tttgaggatc tcgtccggca ataccgcgat aacccgctta acccactcag
     2701 gatcgagcat acctcccgaa cccttcaggc agttgcggat tgtttttcgc cggtgtgaga
     2761 aaagcgcccg gaccacatcg gcataacggt gccggtcact aatgggaaaa atcgggtgct
     2821 ctcttggaat gatcttaact accgtagaat gcacctgggg tctgggtgtg aaacaattcg
     2881 ggggtaattt aaagcagcgc tgcaccgttg cataggtctg caccataatc gagagtctcc
     2941 cgcaatcctt ggtgcctgcc ggtgccagca tccggtctgc aaattcgctc tggtacatca
     3001 ggacggcttc cttaaacccg acctcaagga gacggaaggt gatcttggac gagacggaat
     3061 agggaaggtt ggaaacaact atctcaaaaa aaggcagttc gcaccgtgtt gcatccccgt
     3121 gctggacgat gagttgacca gcagagattt cggttgaaaa acgatcggcc agctcctcgc
     3181 agagattacc gtccagctcc actgcatgga caattgcccc gcgatcgagc agtgcacggg
     3241 taagagctcc gtttccggga ccaacctcga gcactcgttt acctttgagt tcagtaaggt
     3301 ctgctatccg gtctaccgca tgaggatcag tgaggaaatg ctggtcgtga tgtgctctca
     3361 tcgtgtggtg aaaacgtgat acttgacatc agggtcctga agttcttcga ggatccggga
     3421 ctggatcatc cgctccgggt gagggatcga tttgatccgg gctgatatat ctgccaggga
     3481 tgcaaatggc tttttgttgc gctcctctaa aatctcccac atcagcttct ttccgatacc
     3541 cgggagaagg tgcagcatat ggagcttggg ggtaatcgat attgccttgt taaagaacgc
     3601 aacaaaatca ggttcgcggt cttttatcac tttttcaatg ataaaaggga gctctccctt
     3661 agcagtctgc gtgagatcat tatagccaat ccggcgcttg acgcggtcga ttttatcccg
     3721 ctcagcatcg ccaatatata ctttatcgta gagttggatg agtacgttat tcttggggac
     3781 gagctcgagg agtttgaact gctcaagacc catcgcctgg atgatcggtt ctctcttaaa
     3841 aggagggcgt ggatcctccg ggtgtccttt cagcaacaca tccagtacga tcgcattaac
     3901 ctcttttttt tccgccttca tgaagtcaca cctcagaagt gtgccataac gtgttcaata
     3961 attttatcga gttcttccgg tgacagtgta tagcgctctt ttgcaaaaat cgcccgcact
     4021 tcatcccgtg tcttaggcat aatatttgca atcctgtagg ctatttccgg cttcatcttt
     4081 tccattttta aaagatcagc caccagttca tgtgcttttt caggagtggt ctttgcaagt
     4141 ttattggcat gctcaatact gcgcctgaac tcgtaggaca tctccttttc tgctgcgata
     4201 cgcatggatt ctacaccttg caggactccg cgtacctcag ggagcgtgac cctttcttcg
     4261 ctaatgatac tctttacctt catgccaaca ccctgacaga gaatttactt ttgtgctttt
     4321 agatgttgtg gtcgcgctat gacgatttta tctgcatcgc catcccgtat cgtaagcatc
     4381 catgcccgac cgcgctgacc gaggattgtt cccgtaagcc cgtgaaaacg gcgatggggc
     4441 attcctttct gaatgctggg gttacagaca atgtgcaccc ggtcgccgat ctcgtatttc
     4501 cggatcacgc aggtcacggg tggaagcccg cgctcgcgta aatccttctt gaatttatac
     4561 cgtgttttct ttcttggtcc gttatgatgt gccattatct aatctccctc ctttactcct
     4621 tctacctgca cgacatcgag gctgattacg tgcgccggtc tatccaggat ctcggctaga
     4681 ctgggacggg ttcttccccc gtcccccgat accagttctt tgatatagag accggcttcc
     4741 ccgaggattt cgacaacaaa tttgccgtcc tgttccccgg catactctat atcgaggacg
     4801 atccgctcac ggatcttatc tgctctccgg tgagcgaccc tctctggtgt gcgctggtgt
     4861 attgtggcgc cctgtaaggt ttttaaggca tttgcgaact catccgcgga taatgcaccg
     4921 tcaaccccga ccaggatcct gtatttttta tgcgctttgt ttgatttaag ggtttccact
     4981 tccgcacgtt cactccagcg tttgatggta acagaaactc tcccaacggc cgtccggttg
     5041 atctcctgct cgagccaggc aaggtctaaa gagcgcttct ttggttcaac cacttcaagg
     5101 atgaagggtc tgcccgagcc gatcatccgg gcatcaatat cttcccttcc ggcgccatga
     5161 aggacggcat tctgtgcctc aaatgcgatt atgaccggtc ttgcgataag ttcctcaact
     5221 gaatccaggt actgtgcacc ggtgaaattg catttttcgc agccggctcc cctgcaggcc
     5281 cggcagtccc agtgggtctg ggggatgccc cgctcaaatt tctggtacct gccgtaaaaa
     5341 aagacagaat tgatctgcac ttccacccgg cctgatgcgg gatcgaggat agcaacgata
     5401 tctggttttt taaagtccac gttctttcct gtgagtacag atacagcctt tcccacttca
     5461 cggttcactt cagatttgaa aggctcaggt tctgtgagcg agagatcgct ccagaccatc
     5521 tcctcgtttt cagcaatgag cgggggaacc ctgcacccga tcagaaacgt cgagtactcg
     5581 attcccctga ccgcctgtac aacacgatcc gcccattcgg gtatcgtagc aaagaaatta
     5641 ctgcagaccc agcatgtgcc ggaaaatttc gtatacggct ggttctctga aagcgcccgg
     5701 gcgatcttca ggccgcgtcc ccgttcatca ttggaaagac cgtgcgagcg tttcccaaaa
     5761 aaccgcccca ggcaatggtc gcagcattcc ccgtatgcaa gtatcgatgt tacctgttca
     5821 tctagttcca ttggc
//
LOCUS       DASRLV010000142         6115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_210682,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000142 DASRLV010000000
VERSION     DASRLV010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210682"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..1071
                     /locus_tag="VFC43_05710"
     CDS             133..1071
                     /locus_tag="VFC43_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30715.1"
                     /translation="MTIKKYLISVIIAVCLLVVIPPIQAVSDDTPATVIYQTSFTSDP
                     RWQTNNPSTNYWDPNMGMYRFSLEPSTGGYAFVPIDYERGSFTLDYDLMLTRIDDGAT
                     FRFGMAGTEMDPSKAPNVLTVFTNTQAKYGQIMWIHLVTPGSKLIEVSSQTSSEPGAY
                     NGPTVKYELNKTYHISVKYDDDHKTLSMNVNEKLTGKEIWSYYLNTAENLNGMNRLYL
                     GSKGDYGMMSIYAQGYLDNVRLTAPAKITATPTAATTAQSFTTMPTTKLTTKQTTVAL
                     TPYPTDTTQSPSAGILAITALGIAVVCGVLVQKRKD"
     gene            1442..2227
                     /locus_tag="VFC43_05715"
     CDS             1442..2227
                     /locus_tag="VFC43_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HZK30716.1"
                     /translation="MNPSDKSPTIIVHEDSDSLEELSEYLDVLSSSARLRILKFLEKK
                     PRDARAISKEIETSYENTKKHLDKLLSVGIIKKEAGLGAPTSKGIHPVWEYSIVPGGL
                     EAIIRNLGLFSNTRVQIVGSEISRRLEEVKNTLSKELLGNTPSAIALGGPDDGKVFML
                     KQNLVRIGRIDSENQAAYSPQDDIVLSDGYTAVTRVSKPHGRFIRSNEGLQIEDCGST
                     GGTRLNNKRLEKNTPVTLHDGDLVEFSKGVSGVKLLIIIPQAN"
     gene            2356..4416
                     /locus_tag="VFC43_05720"
     CDS             2356..4416
                     /locus_tag="VFC43_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZK30717.1"
                     /translation="MITGLTIIPAATATEFNVGPSGSDFTSIQAAINRAFEGDVIFVH
                     SGTYLENLQLNKKIDLIGEDSGEGAPVIEPVKKGNAIEILADGCRIEGFTIRNIDMAT
                     GIRVNSNENKITKNSFQNNAQGIFLDSAMRNIINRNTITNSSRVGIEIKASNNNLIEE
                     NKFSQNSIGIEVDEYSLSNKVYRNNFDNSQNVVSRSATSVWNTSDTYSYTYLGLQRQS
                     QMGNYWSDYYGKDKNIDGIGDIPNNIKVGANPKSIFDQDIKDSYPLMDPTEYYYDVLR
                     SAPKADIPAPVPLPTALPGSEITSVTTTAPQKTPETTQATPVPTENNGEGQQPTDFLM
                     VELIVLLLAGAVVLIIFRQLKHKESKEEILSLSKEATTVKKISRTSPRPSRTSPRPIN
                     PSATVPDSGTAVPGVPRTYTSIAEQMSYFPIELESKYSEIKYIGRGGIAWVFSAVRKS
                     DGMTVAVKIPISFDEMTGKSFLNEIAAWETLRHPNIVKVTAVNILPVPFVEMEYVPEA
                     LEATPKPIPVWKAVHFIRGVADGLKYAHEHGFIHRDIKPHNILLNKDFVPKITDWGMS
                     KVLANEVKKSSIAGFSLSYAAPEQVSPSEFGRTDERTDIYQLGVVFYELVTGSVPFGG
                     ESIVEVGNAILREQPKPPSHYQADAAVVEKIILKCLEKDPANRYQTASELLEALDGYL
                     EEEV"
     gene            4476..4667
                     /locus_tag="VFC43_05725"
     CDS             4476..4667
                     /locus_tag="VFC43_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30718.1"
                     /translation="MTPAERVYVPATCAHCSGIGCLYCNKTGTVLVLSPKRPCRYCEG
                     VGCIYCGFTGWTGPKGKCD"
     gene            complement(4684..5871)
                     /locus_tag="VFC43_05730"
     CDS             complement(4684..5871)
                     /locus_tag="VFC43_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZK30719.1"
                     /translation="MHGPGHERTIDRGLESSIRTITEKREALLSPLASISADAVRRHN
                     RTSEDIRTPYSRDTDRIIHTRAYTRYIDKTQVFSLVDNDHITHRVIHVQLVSKIARTI
                     GRCLRLNEDLIEAIALGHDIGHIPYGHFGETCLSTLCEEQGIGKFFHNVQSIQFLDRI
                     ENCDLTMQVLDGILCHNGESDDVKIYKEPCRNWLEFDKKVQDNADGRRTKAPMTLEGC
                     VVKFADTIAFIGRDLQDAREVGLISNATPLPRECREILGESNREIINILIYDLLENSD
                     SEEEGYISYSPQVESALISLRSFSRTNIYENEKLTSEHAKVRQMYSTLFISCLEDLEN
                     NTDNAKIFTDFIDTSWIDRTYLDSATYPELVRDFISGMTDRYFQKRYEECVIPRRVEG
                     KFP"
     gene            complement(5934..>6115)
                     /locus_tag="VFC43_05735"
     CDS             complement(5934..>6115)
                     /locus_tag="VFC43_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30720.1"
                     /translation="EGSFGLIPVPENMLKQGEDDIEQQINRQLLAMPGLRIDTLEINN
                     GQLHYRGAFPQSASA"
BASE COUNT         1716 a         1455 c         1430 g         1514 t
ORIGIN      
        1 gcagatgtac actataatta tgccatcacg cagcccggca agggattgtg aatgttccgc
       61 acacacaagc ggcggctgat gaacgagccg ggcctgaacg ctctgtttga gaattaaaat
      121 ggagagaccc ttatgacgat aaaaaaatac ctgatatccg ttattattgc tgtctgcctg
      181 ctggtggtaa taccacccat acaggcagtt tcagatgata ctccggcgac cgtgatttac
      241 cagacctcat ttacctcaga tcccagatgg cagactaata atccatccac aaactactgg
      301 gatccgaata tgggtatgta ccgcttttcc cttgaaccaa gcacaggagg ctacgccttc
      361 gttcccatcg attatgaaag aggatctttt accctggatt atgatctgat gctgaccaga
      421 attgacgatg gggcaacatt caggttcggt atggccggca ccgagatgga tccatcgaag
      481 gctccgaatg ttctcaccgt gtttaccaat acgcaggcaa aatatggcca gatcatgtgg
      541 attcacctgg ttacaccggg aagcaaactg atagaggtaa gcagccagac ctccagtgaa
      601 cccggggctt acaacggacc cacggtaaag tatgaactca ataaaacata ccatatttcc
      661 gtgaaatacg acgacgatca caagactctc agcatgaacg tgaatgaaaa gcttaccgga
      721 aaggagatct ggagttatta cctgaatacc gccgagaacc tcaacggtat gaacaggctt
      781 tacctcggtt caaagggtga ctatggcatg atgagcattt acgcccaggg atatctggac
      841 aatgtccgtc ttactgcgcc agcaaaaatc acagccacac caacagcggc aaccacagca
      901 caatccttta cgactatgcc gacaacaaaa cttaccacaa aacaaacgac cgttgcactg
      961 acaccgtatc cgaccgatac aacccagtct ccttctgcag gcattcttgc aattactgca
     1021 ctgggcatag cagtcgtatg tggtgttctg gtgcaaaaga gaaaggacta accctttttt
     1081 taaaaaatat atagcttaag atgtatgatt cagagggatg aggaatccct ctttctctgg
     1141 agtgagattt tttcaaacgt tccctttttg tctgaaagta tggggttaat ctggttcaat
     1201 gtgcattgga tgggtcggtg acaccccgaa tacctttcgt gcatcacctg agtatatatt
     1261 ataattgcag gggctgataa atacccaaaa tcgtgatggt tggagtattg aaccggtggg
     1321 ttatacctat gaacagaaag gattgatact actgggagtt aatataaact gtcacgacct
     1381 tataatctga ttaccatagg gttatgagaa gacagatcag atgtaaactt ggtggagcgc
     1441 tatgaatcct tcggacaagt cccctacaat tattgttcat gaagattcag actcactgga
     1501 agaactatca gagtatctgg atgtactttc aagtagcgcg cggctcagaa tattaaaatt
     1561 tctcgagaaa aagccccggg atgcccgtgc tatctcaaaa gagattgaga ccagttatga
     1621 aaatacaaaa aagcatcttg ataaactcct gagcgtgggt ataatcaaaa aagaggcagg
     1681 actgggtgcc ccgacttcca agggcattca ccctgtatgg gaatattcaa ttgtgcccgg
     1741 cgggcttgaa gcgatcataa ggaacctggg gctcttttcc aatacgcggg tgcagatcgt
     1801 cgggagcgag atctcccgcc gtctggaaga ggtaaaaaac accctctcaa aagaactgct
     1861 gggaaatacc ccttcggcta ttgctctcgg aggaccggat gacggaaagg tgttcatgct
     1921 gaaacaaaac ctggtccgta tcggcaggat agattccgag aatcaggctg catactctcc
     1981 tcaggatgat atcgttctct ctgacgggta cacagcggtc acccgtgtat caaagcctca
     2041 cggccggttt atccgaagca atgaggggct gcaaatcgag gactgtggaa gcaccggagg
     2101 tacaaggctc aacaataaac gtcttgaaaa aaacacaccc gttaccctcc atgacgggga
     2161 tctcgttgag ttttcaaaag gcgtatctgg tgttaaactt ctcattatca ttccgcaggc
     2221 gaactaaatg cacatgccat tgttcttttg cctcataacc tgagattaaa ccagacatca
     2281 gctaaaaaaa agacaaaaat aaaagtcacg cccatgattt cgatacaccg cattgtatgc
     2341 atcttttttt tccttatgat cacaggcctg accatcattc ctgctgcaac tgccactgaa
     2401 ttcaacgttg gcccttctgg ttcagatttt accagcatcc aggctgcaat taaccgtgct
     2461 ttcgagggag atgtgatttt tgtccacagc ggtacctacc ttgaaaatct ccagctgaat
     2521 aaaaagatag atctcatcgg tgaagacagc ggagaagggg cacccgtaat tgaaccggta
     2581 aaaaaaggca atgcaattga aattcttgct gatggttgca ggattgaagg attcacgata
     2641 cggaacatcg atatggctac cggtatccgg gtcaattcca atgagaacaa aataacaaaa
     2701 aattcattcc agaacaatgc ccaggggatt tttctcgatt ctgcgatgag gaatatcatt
     2761 aaccgcaata ctattaccaa cagcagcagg gtcggaatcg aaattaaagc gtcaaataat
     2821 aatctgattg aagaaaacaa gttttcacaa aattcaatcg ggattgaggt agacgaatac
     2881 tctctttcca acaaagtcta ccggaacaat tttgacaatt cacagaatgt agtgtccagg
     2941 agtgcgacgt cggtctggaa cacatcggat acgtactctt acacgtacct gggtctccag
     3001 agacaaagcc agatgggcaa ctactggagc gattattatg gaaaagataa gaatattgac
     3061 gggatcgggg atataccaaa taacattaag gtcggagcaa acccaaaatc gatcttcgat
     3121 caggacataa aggattcata tcccctgatg gatcccactg aatattacta tgatgtcctc
     3181 cgtagtgccc ccaaagcaga tatcccggca ccggtacctc tcccaacagc acttccaggg
     3241 tctgaaataa catcggtcac gacaactgcc ccccaaaaaa ctcctgaaac aacccaggca
     3301 actcctgtac ctacggaaaa taacggtgag ggacagcagc caaccgactt ccttatggta
     3361 gaactgatcg tgctgctcct tgcaggtgct gttgtcctga ttatattcag gcaactgaag
     3421 cataaagaga gcaaggaaga aattttgtcc ctgagcaaag aagcaaccac tgtgaaaaaa
     3481 atttcccgta cctctccccg cccatcccga acttcacccc ggccaataaa tccctctgca
     3541 acggttcctg attcaggaac agccgtgccg ggtgtaccca ggacctatac ctcaattgct
     3601 gaacagatga gttactttcc cattgaactg gagagtaaat attctgaaat aaaatatatc
     3661 ggacgcgggg gaatcgcatg ggttttttct gccgtgagaa aatcggatgg gatgaccgtt
     3721 gctgtcaaaa tcccgataag tttcgatgaa atgaccggga aatccttttt aaatgagatt
     3781 gcggcatggg agacgctcag gcaccccaat attgtaaaag tgaccgcagt caatatactt
     3841 cctgttccgt ttgttgaaat ggagtatgta cctgaggcgc ttgaggcaac ccccaaacct
     3901 atcccggtct ggaaagctgt tcactttatc cggggggtgg ctgacggtct taaatacgct
     3961 cacgagcatg gcttcatcca cagggatatc aagccccata atatcttact caataaagac
     4021 tttgttccaa aaattactga ctggggcatg agcaaggttc tcgcaaacga ggtaaaaaaa
     4081 tccagtattg caggtttctc tctgtcctat gctgctcctg aacaggtttc cccatcagag
     4141 ttcggcagga ccgatgaacg taccgatatt taccagcttg gagtggtctt ttacgaactg
     4201 gttaccggtt cggttccctt tggtggggag agcatcgttg aagtcggtaa tgcaattttg
     4261 agagaacagc caaaacctcc ttcccattac caggccgatg cggcagtagt tgagaaaatt
     4321 atcttgaaat gtcttgaaaa agatccggca aatcgctacc agacagcatc ggaactgctt
     4381 gaagcgctcg acgggtatct tgaggaagag gtgtgaatgc cgctcatagc gggtgctttt
     4441 tacgatgata acccgtacct cgtgagatgc ataccatgac ccccgcagag agggtgtatg
     4501 ttcccgccac gtgtgctcat tgcagcggga taggttgcct gtattgcaac aagacaggaa
     4561 ccgttctcgt gctctcacca aaacgaccct gcaggtattg tgagggtgtc ggatgcatct
     4621 attgcgggtt taccggctgg accgggccaa aagggaagtg tgactgacac cggggcccgg
     4681 cagttacgga aattttccct ctaccctgcg tggtatcaca cattcttcat atcgtttctg
     4741 gaaatagcgg tcggtcatac cggaaataaa gtcccggacg agttccgggt acgtggctga
     4801 atcaagatag gtccggtcaa tccagctcgt atcgataaaa tctgtaaaaa ttttcgcatt
     4861 atctgtattg ttttcaaggt cctcaagaca gctgatgaac agggtagagt acatctgcct
     4921 gacttttgcg tgttctgagg taagtttttc gttctcgtaa atattagtcc gtgaaaatga
     4981 ccgcagggaa ataagcgctg attccacttg aggactgtat gagatataac cctcctcttc
     5041 actgtcactg ttttcaagca ggtcatagat gaggatattg ataatttccc ggttggattc
     5101 cccaagaatc tcccggcact cgcggggaag cggagttgca ttgctgatca atcccacctc
     5161 ccgtgcgtcc tgcaaatccc tgccaatgaa cgcgatggta tcggcaaact tgacaacgca
     5221 cccttccaga gtcatcgggg ccttcgtccg tctgccatct gcattatcct gaactttctt
     5281 gtcaaactcc agccagttcc tgcagggttc tttataaatt ttaacatcgt cagactcccc
     5341 gttatgacag agaatcccgt caagcacctg catggtcagg tcacagttct cgattcgatc
     5401 gaggaactgg atgctctgga cattatggaa aaactttccg atcccctgtt cttcacagag
     5461 cgtcgacagg caggtctcac caaaatgccc gtagggaata tggccgatat catggccaag
     5521 ggcaatggct tcaatcagat cctcgtttaa gcggagacat cgtcctatcg ttcttgcgat
     5581 ttttgaaaca agctggacat gaatgacgcg gtgcgtgata tggtcattgt caacgagaga
     5641 aaagacctgg gtcttatcaa tataccgtgt atatgcccgc gtgtggataa tacggtcagt
     5701 atcgcgggaa tacggagtgc ggatatcctc agaggtcctg ttatgcctcc gcaccgcatc
     5761 agcgctgatc gaggccagag gtgagagaag ggcttccctt ttttccgtaa tagtacgaat
     5821 tgaggattca agccctctgt ctatcgtccg ttcgtgtccc ggtccgtgca tatcgcacgt
     5881 caattctgtg cccgggcgta ttaatcctga tggaaggggt caggaaaaaa agatcaggca
     5941 gatgctgact gcggaaatgc accacggtaa tgcagctggc cattgttgat ctcaagagtg
     6001 tctattctca gtcctggcat agccagaagc tgccggttta tctgctgttc aatgtcatcc
     6061 tctccctgtt ttaacatgtt ttccgggacc gggataaggc cgaaactacc ttccc
//
LOCUS       DASRLV010000143         8975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_540339,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000143 DASRLV010000000
VERSION     DASRLV010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 8975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8975
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540339"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VFC43_05740"
     CDS             <1..215
                     /locus_tag="VFC43_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK30721.1"
                     /translation="YTIFFAIEDPGKLIGHAKPYRQEEGFFIWEAQDMNELNECTGII
                     KDSGGRVEKIESRYPSLEEMLVKIGK"
     gene            279..815
                     /locus_tag="VFC43_05745"
     CDS             279..815
                     /locus_tag="VFC43_05745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZK30722.1"
                     /translation="MKKPPIPDMLLNTGKRRQEDLVVRKVRESDRDGIIAIFNHYAAT
                     SYAAYPEIPVNDRFFEFLREGTLVFYVLEKTSHIVGFGLLKPVLPFPAFMKTGMLTYF
                     LMPEFTGHGCGNMLLDRLTSDALKMGMTSLVANMASKNIPSIHFHVRNGFTEVGRLQN
                     AGMKFDEQFDIVWMQKML"
     gene            complement(932..1315)
                     /locus_tag="VFC43_05750"
     CDS             complement(932..1315)
                     /locus_tag="VFC43_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HZK30723.1"
                     /translation="MGNYCRGDVVIAPVACEERGVIKTRPAVVIGTGDRGDVYVCPIS
                     SKPASDTHCVPVTLDDFSEGGLDLFGESYILTSRVFIIRNGAVMGKRGRLTSESVSSV
                     LTGIPQSLFPENEKANLKKGSRTLR"
     gene            1421..2062
                     /locus_tag="VFC43_05755"
     CDS             1421..2062
                     /locus_tag="VFC43_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZK30724.1"
                     /translation="MTGYHAHTIGVFEKMEFKDIVMQRYATKKFNGKRISEDKISELL
                     ELVRFAPSALNLQPWKIKIVSDQNIKEQLLPASNNQQQVTTCSHLLVFCADTDYDGLI
                     KRLEALMQKHGIPDEMKKMVIGMSREFTGKMSADQKRAWSQAQTYLALGNALNGAKAL
                     GFDSCPIGGFDPAEYSRILKIPANLVPVMLCPVGYAADKPMPKIRFPREDITF"
     gene            2123..2452
                     /locus_tag="VFC43_05760"
     CDS             2123..2452
                     /locus_tag="VFC43_05760"
                     /inference="COORDINATES: protein motif:HMM:NF013587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AN1-type zinc finger domain-containing protein"
                     /protein_id="HZK30725.1"
                     /translation="MARCDSCGNECTLPFTCQHCGGKFCPECRLPPNHQCTAIRSWKK
                     KPVPGVGMRYGRGNSVTATSSGYAETRHAQAKKRGKGIPWLMIMAAIIIIILLAFFLM
                     VLNGYMR"
     gene            complement(2526..2648)
                     /locus_tag="VFC43_05765"
     CDS             complement(2526..2648)
                     /locus_tag="VFC43_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30726.1"
                     /translation="MQQVSPGDIRLVIRRERNADPFAVNDKGQGYPGDHNCSPY"
     gene            complement(2656..3081)
                     /locus_tag="VFC43_05770"
     CDS             complement(2656..3081)
                     /locus_tag="VFC43_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30727.1"
                     /translation="MPVSRHGRLLFIVLLIVLTIAVAIILNTNNAGLFPANRTGTLTG
                     NLTIGPLCPVEPCTISHDQIVAAYAARPIMITTEGGTFIGSVTADPDSGYSVGLTPGT
                     YVVDIRHQGIGGSGDLPKTITIRPGETVRLDISIDTGIR"
     gene            3345..3536
                     /locus_tag="VFC43_05775"
     CDS             3345..3536
                     /locus_tag="VFC43_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30728.1"
                     /translation="MSKKEKVMKLVDESTGLMECLVCGAKHNAVMKPGPDGKYLPENW
                     ECVNKCSLLHHEEKSPKKT"
     gene            3622..3972
                     /locus_tag="VFC43_05780"
     CDS             3622..3972
                     /locus_tag="VFC43_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30729.1"
                     /translation="MRITRLYSGEDNQSHFDEPEVQLESRGLLGYFTDPTGVKAFFFR
                     EVPAGYRYDWHNVVCREYVITIEGTAEISVSSGEKRRFGKGDVLLAEDVSGKGHTTRV
                     LGKKPWRQVFITLE"
     gene            4070..4351
                     /locus_tag="VFC43_05785"
     CDS             4070..4351
                     /locus_tag="VFC43_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30730.1"
                     /translation="MWLELLNFIFGIAFGFFHHGKEDLTGILRNGAIVGIILGIVLVL
                     VSMFLVPGDLSISLGFLGVFGIFVEIIIFVIIFVAGAFIGDRLESILKK"
     gene            4445..5113
                     /locus_tag="VFC43_05790"
     CDS             4445..5113
                     /locus_tag="VFC43_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HZK30731.1"
                     /translation="MKILAIHGSPRSIHSTTRKLAEFVLKGAAESGAETEMIDLVDYR
                     IIPCTACDACSLNGICVNDDDVPALLLRMQEADGIIFASPVYIDNITGQMKVFFDRLA
                     DAIHYQVLAGKIGCSVATTYESGGEDVVAYLNHVLNYLGIVSVGGISVAHGGKIELVD
                     MHEAEATALGHKLAIAVLKGYTDPPQEEIIADNRRYFHTLVEENKDFRADEYERWVKM
                     NWMK"
     gene            complement(5176..8586)
                     /locus_tag="VFC43_05795"
     CDS             complement(5176..8586)
                     /locus_tag="VFC43_05795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZK30732.1"
                     /translation="MFSILYVDDEPSLLEIGKLFLENSGMFSVDTVTSAPEALLALAK
                     KRYDAIISDYQMPEMDGIEFLKQVRGAGNMVPFIIFTGRGREEIVIQALNEGADFYLQ
                     KGGEPVSQFAELEHKVQMAVLQRKAEASIRDHERREADIINFLPDATFAIDTKGVVIA
                     WNRAMEKMTGVKTSEMLGKDNYEYAIPFYHERRPILIDLVLKDDPVTEGKYPVLSRDG
                     TTLFSEITIPHFNNSSGAELWFTASPLFDTRNTIVGAIESIREITERKQAEEALHESE
                     KRFRDLSDLLPLVVFEVDTQGNLTYANHISFEWFRYSEEDFRQGLTILQMLTQNDRNR
                     AATAIRAMLAGERARGTTAEYLAQRKDGSTFPISIYSSPVIVNGVITGLRGIIVDITE
                     RRKAENDLKNSVAFLNSLIDQSPTPMWISDERGILILINKSCCDLLQVDEAEVIGKYN
                     IFNDNLIEEQGFMSLVRNVFEKGIVARFPITYDTEHLKTLNLDQFVSLVLDVTIFPIR
                     DASGKITNAVIQHTNITERKKAEDALIQSEVRFRSLIQNASDMILILDREGKIIYESP
                     SIEKIMGYPAGENIGKDPLSYIHPDDIVLVKSALQEIYDLKNPGTPTEFRVKKADGSY
                     IWVDSIATNLLDIPAVNGIVVTTRPIEQRKKMETALRDSEEKYRNVVEDQTEMISRFL
                     PDGTHVFVNDAYCRYFSMKRDEILMHRFRPKIPVEDQERVKRFFVSLTQEHPVDTIEQ
                     RIIMPDNSIKWQRWNDRAIFDSSGKVVEYQSVGRDITEKKEAEEALQQRTKELDYRNR
                     IISTLLDTVPIGIFMVEAPSGIPIISNREATRLLGRGIFPDATEKNLAEVYKAFRIGT
                     LERYPTKDMPIVRGMRGESSYIDDMVVIRPDGTTIQLEIFGNPVIDGQGRIVASLASF
                     IDITERKRAEQTIHETNKKINLLTSITRHDVANQVAVLRGFSKIALGKNPDPDIVDLL
                     EKIDTAGATIARQIEFTKAYQELGLHAPGWQRIRKIVAQQKTDGISVSCTCNADVFAD
                     PMIEKVFINLIDNAARHGEHVTTITVSCKPVPDGLVITVEDNGTGVPLDKKEKIFEKG
                     YGKHTGFGLFLAREILGITGITIHETGICGKSARFEIHIPKGKYRGAT"
     gene            complement(8843..8916)
                     /locus_tag="VFC43_05800"
     tRNA            complement(8843..8916)
                     /locus_tag="VFC43_05800"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8880..8882),aa:Lys,seq:ctt)
BASE COUNT         2271 a         2108 c         2222 g         2374 t
ORIGIN      
        1 cctatacgat cttctttgct atcgaagatc ccggcaagct gatcgggcac gcaaaacctt
       61 accgccaaga agaagggttc ttcatatggg aagcgcagga catgaatgaa ctcaacgaat
      121 gcaccgggat catcaaggat tccgggggaa gggtggagaa gattgagtcc cggtaccctt
      181 cactggaaga gatgctcgta aagatcggga aatgatcctg acaactgcca gcctgccggt
      241 ctgactcaca ccggaacttg aataaaagtt gatagaatat gaaaaaaccc cccatacccg
      301 atatgttgct gaataccgga aagcgccggc aggaagatct tgtcgtaaga aaggtgcgtg
      361 aaagtgaccg cgacggtatt attgctattt tcaatcacta tgcagcgacc agttatgctg
      421 catatcctga aataccggta aatgatcggt tttttgaatt tttacgtgaa gggaccctcg
      481 tgttctatgt tctggaaaaa acatcacaca ttgtgggttt cggtttgctc aagccggtcc
      541 tgccgtttcc tgcctttatg aaaacaggaa tgcttaccta tttcctcatg ccggaattca
      601 ccgggcatgg ttgtggaaat atgcttcttg acaggctcac ttccgatgca ttaaagatgg
      661 ggatgacctc gctggttgca aatatggctt caaaaaatat cccgagtatc cacttccatg
      721 tgcggaatgg atttactgaa gtaggcaggc tgcagaatgc cggcatgaaa ttcgatgaac
      781 aattcgatat agtctggatg cagaagatgc tgtgatagtg tcagcggctc tgatattgcc
      841 atgaatcatt tttcacaaac agagccataa atccttacag agccccttgt tcaaggcaga
      901 gacaaagtgg gtttaaccaa aattgatact attatcgcag ggttcgtgaa ccttttttta
      961 agtttgcctt ttcgttttcg ggaaacagcg attgaggtat cccggtcaaa accgatgaga
     1021 cagattcgct ggtcaggcgc ccccgttttc ccatgactgc accattccgg atgataaaaa
     1081 cccgcgatgt caggatataa ctttcgccaa aaaggtcaag acccccttct gaaaaatcgt
     1141 caagagtgac agggacacag tgagtatcgg atgctggctt actgctgatc gggcagacat
     1201 agacatcacc acgatctcct gttccaatca ccactgcagg acgggtcttg atcacacccc
     1261 tctcctcgca agcgacaggg gcgatcacca catcgccccg gcaataattt cccatgcata
     1321 cctagtcagg tgtataaaag cataaggagt ttgatcggca ctggccataa aagttcaaaa
     1381 ggacacccct tatccgaaaa ttgtatatct ataaatattg gtgacaggat atcacgctca
     1441 tactattgga gtgtttgaga agatggagtt caaagacatc gtgatgcaga ggtacgcaac
     1501 gaaaaagttc aatggaaaaa ggatcagtga ggacaagatc agcgagcttc tggaactggt
     1561 aagattcgcg ccctctgcac tgaaccttca gccatggaag atcaaaatag tttcagacca
     1621 gaacataaaa gagcaactcc tcccggcatc gaataaccag cagcaggtca cgacctgttc
     1681 tcaccttctg gtcttttgtg cggacaccga ttacgacgga ctgatcaaaa ggcttgaagc
     1741 cctcatgcaa aaacacggaa ttccggatga aatgaaaaaa atggtaattg ggatgtcacg
     1801 ggaattcacc ggaaaaatgt cagcagacca gaaaagagca tggtcgcagg cacagacgta
     1861 tcttgccctc ggcaatgctc tcaacggagc aaaagcgctc ggatttgatt cctgtccgat
     1921 agggggtttc gatccggcag agtactcgcg tatcctgaag atcccggcga atctagtccc
     1981 ggtgatgctc tgtccggtcg gttatgccgc tgataaaccg atgccaaaaa tcaggttccc
     2041 gagggaagat atcacatttt aaagaaaagg aaacgggcac aggaatcatc tttattaatg
     2101 gtaaaaaacc tattgctctg tcatggcacg ctgtgattcc tgtggcaatg aatgcaccct
     2161 gccatttacc tgccagcact gcggaggaaa attctgcccg gaatgccggc tgccccctaa
     2221 tcatcagtgt accgccatca ggtcctggaa gaaaaaaccc gtacctggcg tgggcatgcg
     2281 ctatggcagg ggtaatagcg tgactgctac cagcagcggg tatgctgaga cacgacatgc
     2341 tcaggcaaag aaacgtggta agggaatacc gtggctgatg atcatggcag caattattat
     2401 cattatcctt ctggcatttt ttctgatggt actaaacggg tacatgcgtt agcatcatac
     2461 ctatgctcac ggttcgccat ctcgttgttg tcattttggg atctgaattg atgagcgcat
     2521 agtttttagt aaggcgaaca gttgtggtca ccgggatatc cctgaccttt gtcattgact
     2581 gcgaaaggat ctgcgttgcg ttcgcgtctg atgacaaggc gtatatcccc cggggacacc
     2641 tgctgcatgc tggtatcacc gtatgccggt atcgatggag atgtcaagcc tgaccgtttc
     2701 tccggggcgg atggtaatgg tctttggcag atcaccactt ccaccgattc cctggtgccg
     2761 gatatccacc acatatgttc cgggtgtgag gccgactgaa tagccgctgt caggatcggc
     2821 cgtgacggac cctatgaaag tacccccttc tgttgtgatc ataatcggcc gggctgcata
     2881 tgctgcaaca atctggtcat gtgagatggt gcagggttca accgggcata ggggccctat
     2941 tgtgagattg ccggtcaggg tgcctgttct attggcggga aacaaccccg cattgtttgt
     3001 gttcaggata atggcgactg cgatggtaag gacaataagg aggacaataa acaggaggcg
     3061 gccgtgtctg gatactggca taatggtttg attgcggtga tgggaaaaaa gtgttgcggc
     3121 accagagatc gtacccgaaa tgttacctct ctgaaagaac ccttgttttt ggggctattc
     3181 agaacagcgg tatgggatgg gaaaagggct acgatgttcg ccagatgacc attatttcca
     3241 aaaaattatc tggtgttgag taattctctt cccatagtaa gcataatcca gacccgacta
     3301 cccttccatg aatttatcac tcttaaggtt gttacattgg aaacatgtcg aagaaagaaa
     3361 aagtgatgaa actggttgac gaatcgacgg gactcatgga atgccttgtg tgcggggcaa
     3421 agcataatgc ggtcatgaaa ccgggacctg acgggaaata cctgcctgaa aactgggagt
     3481 gcgtcaacaa atgttcattg ctccatcacg aagaaaaatc tccaaaaaag acataatacc
     3541 ttttcccact tcatatcccg cggtatgccg gtttacattt cggaattttt aacatcagga
     3601 aaacctacca aaggatagaa tatgcgaatt acgcgcctgt attcgggaga ggacaaccag
     3661 tcccattttg acgagcctga agttcagctg gaatcccgtg ggctgctggg ctattttacc
     3721 gatccaaccg gcgttaaagc gttcttcttc cgggaagtcc ccgccggata ccggtatgac
     3781 tggcataatg ttgtctgccg tgagtatgtg ataacaatcg aaggaacagc tgaaatttca
     3841 gtgagcagcg gtgagaaacg ccggtttggg aaaggcgatg tcctccttgc cgaggatgtt
     3901 tccggtaaag gtcatacgac ccgggtatta ggtaaaaaac cctggcgaca ggttttcatc
     3961 acccttgaat aaccgtaatc ctttctgata ctcaaagaca tgattcctgc tcgtattcca
     4021 aaccttttta gcgacggcgc gcgatattgc ttaaaccagg agaatccgga tgtggcttga
     4081 actgctgaat tttatctttg gtatcgcctt tggctttttt catcatggga aggaagacct
     4141 cacgggtatc ctcagaaatg gtgcaatcgt cgggatcatt ttgggtattg tccttgtgct
     4201 cgtctcgatg ttccttgttc ccggggacct gagtataagc cttggtttcc tgggagtgtt
     4261 tggaattttt gtcgagatta tcatctttgt gattatcttt gtcgctggtg ccttcattgg
     4321 tgacaggctt gaaagtatcc tgaaaaaata accttgccgg atgccggaac gtctcctgtg
     4381 gcgaggaaac tccctctcat ccataataat ccccgacccc ttgtgataaa agaaagagcc
     4441 ccccatgaag atccttgcaa ttcacggcag tccccgatca atacacagca caacacgaaa
     4501 gcttgcggag tttgtgctta aaggagcagc agaatccggc gcagagactg agatgatcga
     4561 ccttgtcgat taccgcatca taccctgcac tgcctgcgac gcctgctcgc tgaatggtat
     4621 ctgcgtcaac gatgatgatg ttcccgcgct cctcttacgg atgcaggagg cagatggcat
     4681 aatctttgcc tcgccggtgt atatcgataa catcaccggc cagatgaagg tatttttcga
     4741 caggcttgcg gatgctatcc actaccaggt acttgcagga aagatcgggt gttcggtagc
     4801 gacgacctat gaatccggag gggaagatgt tgttgcatac ctgaaccatg tgctcaatta
     4861 tcttgggata gtatccgtgg gcggaatcag tgttgcccat ggggggaaaa tcgaacttgt
     4921 tgacatgcat gaggcagagg caaccgccct tggccacaaa cttgcaattg cagtcttaaa
     4981 aggatacacg gatccgccgc aggaggagat aattgctgac aaccgcaggt atttccatac
     5041 tctcgtggaa gagaacaagg attttcgcgc cgatgagtat gaacggtggg taaaaatgaa
     5101 ctggatgaaa taatcacgtc tgacgatgca gcttgcggtg tccggtgcca gatccgatca
     5161 ttcttaaagc aggactcagg ttgccccccg gtattttccc ttcggtatat ggatctcaaa
     5221 ccgtgcactt ttaccgcaaa tgcccgtctc atgtatcgtg ataccggtga tgccgaggat
     5281 ttcccgggct aaaaagaggc caaagccggt gtgtttgccg tatccttttt caaatatctt
     5341 ctctttttta tcaaggggaa cacctgtccc gttatcttca acggttatta caagaccgtc
     5401 cggcactggt ttgcagctga cggtgatggt agttacatgc tccccgtgcc gggcagcgtt
     5461 atcgatgagg ttgataaaga ccttctcaat catggggtca gcaaatacat cggcattaca
     5521 ggtacacgac accgatattc catcagtttt ttgttgtgca acgattttcc ggatccgttg
     5581 ccagccggga gcatgcaagc ccagttcctg gtacgccttg gtgaactcaa tctggcgggc
     5641 aatcgtggct cccgccgtat cgatcttttc cagcagatca actatatcag gatcagggtt
     5701 cttcccgagg gcaatctttg aaaatccgcg tagtactgcg acctggtttg caacatcgtg
     5761 gcgggtgata ctggtcagga gattgatttt tttgttggtc tcatgaattg tctgttctgc
     5821 ccgttttcgt tctgtgatgt cgatgaaact ggcaaggctt gcgacgatac gaccctggcc
     5881 gtcgattacg ggattcccga atatctccag ttggattgtt gtaccatcag gccggatcac
     5941 taccatgtca tcgatataac tgctttcccc cctcattccc ctgactatcg gcatatcttt
     6001 tgtgggatac cgttccagtg ttccgatcct gaaggcttta tagacttctg caaggttttt
     6061 ttcggtcgca tcaggaaaaa ttccccttcc gaggagccgg gtcgcttccc tgttggagat
     6121 aatgggtata cctgacgggg cttctaccat gaaaattccg ataggaactg tatcaaggag
     6181 agtgctgatg atccggttac gataatcgag ttctttggtc ctctgctgga gtgcctcttc
     6241 tgcttctttc ttttcggtta tatcgcgccc gacagactga tattcaacta ccttccctga
     6301 ggaatcgaaa attgcacggt cgttccatcg ctgccacttt atgctattgt ccggcatgat
     6361 gatccgctgc tcgatagtgt cgaccgggtg ttcctgggtc agggacacaa agaaacgttt
     6421 cacccgctcc tggtcctcaa caggtatctt tggccggaaa cggtgcatga ggatttcatc
     6481 acgtttcatg ctgaaatacc ggcagtatgc atcattgaca aagacatgcg ttccatccgg
     6541 taaaaaccgt gagatcattt cggtctggtc ttcgaccaca ttcctgtact tctcctcgct
     6601 atctcggaga gcagtttcca tttttttgcg ctgttcgatt gggcgggtcg ttactacaat
     6661 accgttcact gcagggatat ccagcaggtt tgttgcgata gaatcgaccc agatatatga
     6721 tccgtcggcc tttttcaccc tgaattctgt cggggtaccc ggatttttca ggtcatagat
     6781 ctcctgaagg gcggatttta ccagtacaat atcatccggg tgaatatagc tcaggggatc
     6841 ctttccgata ttttcaccgg cgggataccc catgatcttc tcgattgagg gcgattcata
     6901 gatgatttta ccctcacggt ccagaataag gatcatgtct gaggcgttct ggataagaga
     6961 acggaaacgg acctcgctct gaataagggc atcttcagct ttcttgcgct cggtaatatt
     7021 ggtgtgctgg atgacggcat tcgtgatttt tccgcttgcg tcacggatgg ggaaaatagt
     7081 cacatcgagt acgagggata cgaattgatc caggttcagg gttttcaggt gctctgtatc
     7141 ataggttatc gggaaccgtg ctacaatccc tttttcaaag acatttcgga caagggacat
     7201 aaaaccctgt tcctcgatca ggttatcgtt aaaaatgtta tattttccaa tgacctcagc
     7261 ctcatccacc tgtaacaaat cacaacagga cttattgatg agaataagta ttcctctctc
     7321 gtcagaaatc cacattggtg tcgggctctg gtcgatcagg ctgtttaaga acgcaaccga
     7381 gttcttaagg tcattctccg cttttctgcg ttcagtgata tctacgatga tacccctgag
     7441 gccggtgatc acgccattga caattaccgg tgatgaatag atggatatgg ggaaagtact
     7501 gccatctttc cgttgcgcca gatattctgc tgttgtccct cgggctctct ctcctgccag
     7561 catagcccgg atagccgtcg cagcccgatt tcggtcgttc tgggtaagca tttgcagtat
     7621 cgtcagtccc tgcctgaaat cttcctctga atacctgaac cattcgaatg agatatggtt
     7681 tgcataggtc aggttacctt gcgtgtcaac ttcaaatacg accaggggaa ggagatctga
     7741 gagatcccgg aaccgtttct cactctcatg aagcgcttct tctgcctgtt tccgctccgt
     7801 gatctcgcgt attgactcaa ttgcaccgac aatggtatta cgggtgtcaa acagcggaga
     7861 agcggtgaac cagagttcgg ctcccgagct gttgttaaag tggggaatgg tgatctcgga
     7921 gaaaagtgtt gtcccgtccc gggataatac cgggtatttt ccttcggtta ccggatcatc
     7981 tttcagcaca aggtcgatga ggatagggcg gcgttcatga taaaacggga tggcatattc
     8041 ataattgtct tttccgagca tttctgatgt ttttacaccg gtcatcttct ccattgcacg
     8101 gttccacgcg atcacgacac cctttgtatc gattgcaaag gtcgcatccg gcaggaagtt
     8161 gataatatcc gcttcacggc gctcgtgatc gcggatgctg gcctctgctt ttctctgtaa
     8221 gaccgccatc tgtaccttat gttccagctc ggcgaactgg gacaccggct cccccccttt
     8281 ctggagatag aaatcggcac cttcgttcaa tgcctggatg acgatctctt cccttccacg
     8341 gccggtgaaa ataatgaagg gtaccatatt tccggctccc cgtacctgtt ttaagaattc
     8401 aatgccgtcc atctccggca tctggtaatc ggaaataatg gcatcgtacc ttttttttgc
     8461 tagtgcgagg agtgcttctg gagctgaagt aacggtatca acggaaaaca taccgctgtt
     8521 ctcaagaaac agtttgccaa tctcgagtaa agatggttca tcatctacgt aaaggataga
     8581 gaacatgcga tgacctgtat gattcggatt ttaggatgac agcaattggt ttcttaacca
     8641 atgaatgtta cgaccatgaa aaaatcttaa agatgtcagc cagccggacg aaagtaaaaa
     8701 gacagtcagt tctctttatg gtaattatct ggaagtttaa gaaaaaagac tgatcgaaag
     8761 aattcttgta tccaggggtc atttcactta gatggcaggc agcctgatta ttccaataac
     8821 ccggaagaaa caacaaaaga aacgggcctg aagggatttg aacccttgac ctgcggatta
     8881 agagtccgcc gctatgccga actaagctac agacccaagc ttgtttgacc tagtaatgtg
     8941 gttgataatg ccgaataaat gttttggagt aattg
//
LOCUS       DASRLV010000144         3306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_864142,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000144 DASRLV010000000
VERSION     DASRLV010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864142"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VFC43_05805"
     CDS             <1..769
                     /locus_tag="VFC43_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HZK30733.1"
                     /translation="SYPERPRESSTFEFMHGTVSPERPSETLIAQIAREILDIRKQGG
                     KIAIVGGPAIIHTGADKALAEMIRKGYIDVLFAGNALATHDIEYNLFGTSLGMDISTG
                     KPVMGGHKHHLYAISEIMRAGSIRNAVENGVVTGGIMYECIRKNIPFVLAGSIRDDGP
                     LPDVITDVMVAQDAMRVHIKGCSMVLMIATLLHSIAVGNCLPSNVKTVCVDINPTSLT
                     KLMDRGTMQAIGVVSDAGTFLPLLSKQLEIQSSHAKK"
     gene            complement(783..1772)
                     /locus_tag="VFC43_05810"
     CDS             complement(783..1772)
                     /locus_tag="VFC43_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosine biosynthesis radical SAM protein
                     RaSEA"
                     /protein_id="HZK30734.1"
                     /translation="MISELFEKPLASWLGQERYNNELLTCLTIIFKSAGCTWSKCRMC
                     SYRHERYNGHQPKEVLLSHLRAQLAWINREYKTDDYRMVKIFTSGSFFDPDEIPPVFL
                     SDVATRFRGKLVIAETRPEFVNADVISPFMEMLDDGSWKIPLYCAIGLETSNDRIREK
                     CINKGFNFSDFITAAKNAHSAGAGVKAYLLFKPLFLTEAEAVADMKTSINETSFHAEI
                     ISMNPCTVQRKTELEYYWKRGAYRPPYLWSILSLLADTPLHITCDPLGGGKNRGPHNC
                     KKCDYDIVNGIRDYSLNADRDLIIALLDTRCDCKNEWEYVLSSEKPYCMPLTS"
     gene            1801..2886
                     /locus_tag="VFC43_05815"
     CDS             1801..2886
                     /locus_tag="VFC43_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30735.1"
                     /translation="MKKIVLLMLGLFFLSGFGSAYRVNIDAPDTLPIGKPLIVNGTTT
                     FGIGTPIDVVLYYQLTTTTEIKRKIVYVQSDNSFRAVFDTTGLKTGMYKVEVPTNGMG
                     DSTNMRVVYLVDRAEDIQLTSPASQNFNGKILISGSIKGDENSGVQIEVIGPDNFVVF
                     GPRFINTNYQGTFTTEVPISEPGEFEVSFTDARGYVGKRIIMVNGQQSLTSAPSLVPT
                     TVSVISAHTKSSRDNPAYFAIKTGFGQVTIHTSSSIDWIIEYVDENGVIQLVNGQGEQ
                     YPEQFQVQGKGKIVYIKVYPVKYSVNSEVFLYAENAISITVPSAAPEPFAARTQTTQT
                     SAPGSPLYPVIVIIALGVAVMGIRKKV"
     gene            2986..3070
                     /locus_tag="VFC43_05820"
     tRNA            2986..3070
                     /locus_tag="VFC43_05820"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3020..3022,aa:Ser,seq:tga)
BASE COUNT          917 a          784 c          754 g          851 t
ORIGIN      
        1 cagttatcct gagcgtcccc gtgagagcag tacctttgag ttcatgcatg gcacagtatc
       61 gccggaacga ccgagcgaga ctctgattgc ccagatcgca cgggagatac ttgatatccg
      121 taaacaggga ggtaagattg ccattgttgg cggaccggca atcattcata ccggtgcgga
      181 taaggcactt gccgagatga tccgcaaagg ctacatagac gtcctctttg ccggaaatgc
      241 ccttgccacg catgatatcg aatataacct ctttggcacc tctcttggga tggatatctc
      301 gacaggaaaa cccgttatgg gaggtcataa acaccatctt tatgcgatca gtgagatcat
      361 gcgggccggt tctatcagaa acgctgtaga aaacggcgtg gttaccggag ggatcatgta
      421 tgaatgcatc aggaaaaata ttccgtttgt ccttgccggt tcaatccgtg atgacggtcc
      481 gctgccggat gttatcaccg atgtcatggt agcacaggac gcgatgagag tccatatcaa
      541 ggggtgcagc atggttctca tgatagcaac actccttcac tctatcgcag ttggtaactg
      601 tctgccatca aatgtaaaaa ctgtttgtgt tgatatcaac ccgacatcac tcaccaagct
      661 gatggaccgg ggaacaatgc aggctatcgg tgttgtttcg gatgcaggaa cctttctccc
      721 gctcctgagt aaacagctcg agatccagag cagtcatgca aaaaagtaac cgtatcagac
      781 agttaactgg tcaggggcat acaatagggt ttttctgacg aaaggacata ttcccattca
      841 ttcttgcagt cgcaacgggt atcaagcagg gcaataatga gatccctgtc agcgttgagc
      901 gaataatcgc ggatcccgtt cacaatatcg taatcgcact ttttgcaatt gtgaggtccc
      961 cggttcttcc cgccaccaag aggatcacag gttatatgaa gaggggtatc tgcaagcagc
     1021 gaaaggatac tccaaagata aggaggacgg taagcaccgc gtttccagta atattccagt
     1081 tcggttttgc gctggaccgt gcagggattc attgagataa tctctgcatg aaaagatgtt
     1141 tcattgatgg atgttttcat gtcagccaca gcctctgctt ccgtaagaaa aagtggtttg
     1201 aagaggagat aggctttgac tcctgcaccg gcagaatggg cattttttgc agctgttatg
     1261 aaatctgaga aattaaaacc tttattgatg catttttccc ggatcctgtc attgctcgtc
     1321 tcaagtccta ttgcacagta gagcggaatt ttccagctcc cgtcatccag catttccata
     1381 aatggcgaga taacgtccgc attcacaaat tccggacgtg tttctgcaat caccagtttc
     1441 cccctgaacc tagtagcgac atccgacagg aaaacagggg gtatttcgtc agggtcaaaa
     1501 aaactcccgg atgtgaagat tttcaccatg cggtaatcat cggttttata ttcgcggttt
     1561 atccatgcca gttgtgcccg caggtgcgaa aggagcacct cttttggctg atgcccgtta
     1621 taccgctcat gacggtaact gcacatcctg cactttgacc atgtgcagcc tgcactttta
     1681 aaaataatag taaggcaggt gagcaattca ttgttatacc gctcctgtcc cagccagctc
     1741 gccagtggtt tttcaaaaag ttcagaaatc attaacatca taatagttga acgtatatag
     1801 atgaaaaaga tcgtcctgct gatgttggga cttttctttc tgtcgggatt cggatcggcg
     1861 tatagagtaa atatagatgc gccggacacg ctccccatag gaaaacccct catagttaac
     1921 gggacgacca catttggtat cggcacacct atagacgtag tgctctatta ccagcttaca
     1981 actacaacag aaataaaacg caaaatcgtg tacgtccagt ctgacaatag tttccgtgct
     2041 gtttttgata caaccggttt gaaaacagga atgtacaaag tagaagttcc caccaatggt
     2101 atgggtgatt cgacaaacat gcgtgttgtg tatctcgttg acagagccga agatatccag
     2161 ctcacttctc ctgcatcaca aaatttcaat ggtaaaatct tgatttccgg ctcgattaaa
     2221 ggtgatgaaa attccggtgt ccagattgaa gtgatcgggc ccgataattt tgtcgtattc
     2281 ggaccccgct tcatcaatac caattaccaa ggaaccttta caactgaagt acccatttcg
     2341 gaacccgggg aatttgaagt gagtttcacg gatgcccggg gatacgtggg aaaacgcatc
     2401 ataatggtaa atggacaaca atccctcaca tcagcaccga gtctggttcc gacaacagtt
     2461 tccgttatat ctgctcacac gaaatcctcg agggataatc ctgcctattt cgcgataaag
     2521 accggttttg gacaagtcac cattcatacg tcttcatcga ttgactggat catagaatac
     2581 gttgatgaaa acggagtaat ccagttagtg aacggacagg gtgaacaata cccggaacag
     2641 ttccaggttc agggaaaggg taaaatagta tatattaaag tttatcccgt aaaatattca
     2701 gtgaacagcg aggttttcct gtatgctgaa aatgccattt ccattactgt accctctgca
     2761 gccccggaac cttttgctgc aagaacccag acaacgcaga catctgctcc cggttctccc
     2821 ctctaccccg tcattgttat cattgctttg ggagtcgctg tcatgggaat caggaaaaaa
     2881 gtttgaaaag attgttgatc atcatttcat cagatcaaaa acctgcatac gggtgcacga
     2941 ataaagaagc cttttttata tctgacacct aacatatgga gcactgccgg ggtagtctag
     3001 tccgggaagg cggtagcctt gaaagctact ggtgccctgc acctcaagag ttcaaatctc
     3061 ttccccggcg taagaaagga cggtacctaa ttctgacatt tatttataaa gggttcacgc
     3121 gcactgctta atcaatgaag caaaaccacc aacccaacaa cacccagaac cgttttcata
     3181 aacctgatga agcacatact gggttacccc aaagcggcgt tgatacaccg ccgggctggt
     3241 caacagccca aattccatct ttccaggatt gggcctttcc tcgtaccaaa attagagacc
     3301 ccaatc
//
LOCUS       DASRLV010000145         2644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_296939,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000145 DASRLV010000000
VERSION     DASRLV010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296939"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1778)
                     /locus_tag="VFC43_05825"
     CDS             complement(<1..1778)
                     /locus_tag="VFC43_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012108024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HZK30736.1"
                     /translation="MSEKKQLAIVVIASLIVGIIGLFLIAIIPPLFEGNLVVDTYDAV
                     LYANGTLNERYTYDVKTSGEYRMLFRSWNAPLTFGTAAGQNVQFVSVNPPSGTIGYAK
                     DVGGNVIVTDGPESSAFKSTITTLAETNEVGIFNPDYYSDGKYTVEYSYILHPPLEYD
                     STTSHLNLKFAGGSHIPYRTIRITIPAKNVEQVYVYPPFMVVRNDGDTYTITGSAAAD
                     ENVAVELLATADGLSAIPGIRTEVADLKSKTASGSFWYNLPYFVASLLNILAKACVIL
                     VPILLITLYYHYGREKMFTVPEYLSTIPNPSLKPWQVNLLFKDDALDFDEDGYYATLL
                     ELHRRKLIHITEKGTGKGIEIQILSTATSDPYELRVLAFIQQLSENNVLDTDKIEALA
                     KKAQSNRTAEEKALQYQRMLADVTSRADAALPAQYIVDGREHIIPLLLTAIVLFAITL
                     IFAFVTTMQSSLLIPAVILWAAVLVQVIIAIAAPSTLFGHWKDDKYKEKLEWDAFSHF
                     LSDLTMIQKYAPADISMWGAWLVYGTALGVGENVEKAMKSLNIRVNDTGVPLGAVGMN
                     YAFLPLLHFTPPSHGGSGGGGGFGGGG"
     gene            complement(1791..2354)
                     /locus_tag="VFC43_05830"
     CDS             complement(1791..2354)
                     /locus_tag="VFC43_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZK30737.1"
                     /translation="MDIIIWIVLGVLALVIIGLIFWAVGIYNRFFSLKNSSEATLGQI
                     RVAMKKRLDMIEQLLGAVKSYAKFEKETFERVTAMRASVATASPGDLNKVEAESRSIF
                     GRLLAVAENYPDLKTNTTVMSLMDAVKGVEDEIARQRYTYNNISQEFNTMMDTIPSNF
                     IGRLIGLIKLEYLQFEEAIKTPPKIEF"
     gene            complement(2453..>2644)
                     /locus_tag="VFC43_05835"
     CDS             complement(2453..>2644)
                     /locus_tag="VFC43_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012108026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HZK30738.1"
                     /translation="IYAGHWMQFIPTRLEKVVVEGDWRMPAITLTMEKALIYPPGARV
                     VLHYLEGGKLRVVGSLVIS"
BASE COUNT          695 a          617 c          624 g          708 t
ORIGIN      
        1 ccgccgccac caaagccccc tccgccaccg gatcctccgt ggcttggggg ggtgaagtga
       61 agcaggggaa gaaatgcata attcataccg acagctccca aaggcacacc ggtatcattt
      121 acccggatgt taagggactt catcgccttt tcaacatttt cgccgacccc aagtgcagtc
      181 ccgtaaacga gccatgctcc ccacattgaa atgtccgcag gagcatattt ctgaatcatg
      241 gtgaggtcag aaaggaaatg ggaaaatgcg tcccattcca gtttttcctt gtacttgtcg
      301 tctttccagt gtccaaacag ggtagaggga gcggcgatag caatgatgac ctgaacaagc
      361 actgctgccc agagaatgac tgccgggata aggagagagg attgcatagt tgtgacgaat
      421 gcaaagatga gcgtgatcgc aaacaggacg attgcggtta acagaagggg aatgatatgt
      481 tcccgtccat cgacaatgta ttgcgccggg agtgctgcat ctgctctgga tgtcacatcg
      541 gcaagcatac gctggtactg caatgccttt tcttctgcag tacgattgct ttgcgctttc
      601 tttgccagtg cttcgatttt gtcagtatca agaacattat tctctgatag ttgctgaata
      661 aaagcgagca cccgtagctc gtagggatct gatgttgccg tagaaagaat ctggatctca
      721 atacctttcc cggttccttt ttctgttatg tgaataagtt tccggcggtg taattcgagc
      781 agtgttgcat aatagccatc ctcgtcaaaa tcaagcgcat cgtctttgaa caggaggttc
      841 acctgccagg gcttaagaga cggattcgga atagtgctca gatattccgg aacggtgaac
      901 atcttctccc tgccatagtg ataatacagg gtgatcagga gaattggtac cagaatgacg
      961 caagcttttg caaggatatt cagcagactt gcaacaaaat atggcaggtt ataccagaaa
     1021 cttccggatg ctgtcttact tttaaggtcc gccacttccg tcctgatacc gggtatagcg
     1081 gaaaggccgt cagccgttgc caggagttca actgccacat tctcatcggc tgccgcgctg
     1141 ccagtgatag tgtaggtatc cccatcattc cggaccacca taaaaggggg gtatacatag
     1201 acctgttcca cattttttgc cgggatggtg attcttatgg tacggtatgg gatatgactc
     1261 cccccggcaa atttcaggtt aaggtgcgat gttgttgagt catattcaag gggtggatga
     1321 aggatgtagg aatattcaac ggtatattta ccatcagagt agtaatccgg attaaatatt
     1381 cccacttcat tggtttcagc aagagttgtg atagtcgatt tgaatgcact gctttcagga
     1441 ccatcggtga ctatcacgtt acctccgaca tctttggcat agccgatagt accggacggc
     1501 ggatttactg aaacgaattg tacgttctgg cctgcagcgg ttccaaaggt gaggggggca
     1561 ttccatgacc ggaagagcat ccggtactcg ccggaggtct taacgtcata cgtatatctc
     1621 tcgttaaggg tcccgttggc atacaggact gcatcatagg tatccaccac caggttaccc
     1681 tcaaaaagcg gtggaattat tgcgataagg aatagaccga taattccgac gatcagactc
     1741 gcgattacca ctatcgccaa ttgttttttt tcactcacaa caattacccg ttagaactca
     1801 atcttcggtg gggtcttgat cgcttcttca aattgcaggt attcaagctt tatcagcccg
     1861 attaagcgcc ctataaaatt tgagggaatt gtatccatca tcgtgttaaa ttcctgtgag
     1921 atattgttgt acgtataacg ctgccgggca atctcgtctt cgactccctt taccgcgtcc
     1981 atgagactca tgacagtcgt attggttttc agatccggat aattttctgc aaccgccagt
     2041 aaacgtccga atatcgaccg ggattcggct tcaactttgt tcagatcgcc cggcgaagct
     2101 gttgcaacac ttgcccgcat tgctgttaca cgttcgaagg tttctttttc gaatttcgca
     2161 taactcttca ctgcaccgag aagctgttcg atcatgtcaa gccgtttctt catggcaaca
     2221 cggatctgtc cgagggtggc ttcggatgaa tttttcaggc tgaagaaccg gttgtagata
     2281 ccaacagccc agaagatcag tccaatgatt acaagggcta aaacacccag cacaatccaa
     2341 atgataatgt ccatagttat cattgtactg atattctttc cagtacataa gctattagga
     2401 aattttttga aaaaagctga agacaggaag ataaaaaaaa ttttcagata agttacgata
     2461 taacaagact ccccacaaca cggagtttgc caccttcaag ataatgaagg actacacgtg
     2521 cacctggcgg gtatatcaat gccttttcca tggtaagtgt aattgcgggc attctccagt
     2581 ctccttcaac cacgactttt tcaagacggg taggaataaa ctgcatccag tgaccggcat
     2641 agat
//
LOCUS       DASRLV010000146         4338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_212686,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000146 DASRLV010000000
VERSION     DASRLV010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212686"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            590..1576
                     /locus_tag="VFC43_05840"
     CDS             590..1576
                     /locus_tag="VFC43_05840"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZK30739.1"
                     /translation="MVTFCPKCGTQAVDGQSQFCNMCGSELPADISEETGKYCPNCTA
                     KITDREAVSCARCGFQLLPKKPLTPSVRPTRQCPQCGNPVVDENRFYCNSCGAYIKNT
                     KAGNVSLSDGPAGSKTGIKKPVIIPGIHQNMETKTVTEPGSVVAKRNFDGKNPIIKKI
                     GIVMVVIVGIALLTWVGSMLLGSVSLLPSDNEVLVTQDLDSITLQLSDLPSGWMSGDA
                     VGTDDAYSAQFFTSSENSEALVEETITRFPGIEEARLELNSERQQVTGLTVETINLGN
                     GGFSYIDVNYVMVIFRRGNIIVKIEDTRTEYQENPTMNNAKNYAEIVAGRIK"
     gene            1697..2386
                     /locus_tag="VFC43_05845"
     CDS             1697..2386
                     /locus_tag="VFC43_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HZK30740.1"
                     /translation="MGILIDDPRLRDQRCVFSDRHDAGRKLGALIQSRPFLEDPFVMA
                     IPAGGVPVGVEIARALHAPFSLMVVRKIQVPGNTEAGFGAVTFDGHVLINEHLRSVLD
                     LSGEEVDTAIAATRRNVRERVARYMSGRPFPDPAKKCVVLTDDGLASGYTMLAAVESI
                     RTSHPERIIVAVPTASASSAGRIAREVDECICLNIRSGNRFSVAEAYRNWYDLDDREV
                     LAELRAYQGQT"
     gene            2484..3263
                     /locus_tag="VFC43_05850"
     CDS             2484..3263
                     /locus_tag="VFC43_05850"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HZK30741.1"
                     /translation="MNRSLLSSAIFCLLVLAAFAAGCTNAKQANTTPLPSSVTGSMIS
                     ITADQKTYPAYISVPTFTGKHPGIILLHSFNGLEPGYKVMCDRMATDGFVVIAPEWQT
                     FDRQAGDPEVGAVVRSSIAALKTREDVDAQKLGLIGFCAWRRFTMLFLPQFKDFGAGV
                     AFYGFPYSRGNTNTTTPAGHIGELSTPILVIHGFRDQASPISGIYNYTKELDAADKYY
                     ELKVYQGKPHGFMIVNGTFGQDTAAKDAYSEMTGFFRRMLE"
     gene            complement(3307..3954)
                     /locus_tag="VFC43_05855"
     CDS             complement(3307..3954)
                     /locus_tag="VFC43_05855"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HZK30742.1"
                     /translation="MIGKIVICLLVLSVFLCGCTTTPEEKTGVIQITSTPSGAGAYLD
                     SEYHGTTPVTVKAVPAGNHTVEIRERGYNTWSSIITVTSGGTVPVSTSLVPVSVSTPT
                     TTPTIKPTVAKKDTPELHVDGYWTYPAVRSFSNPIVLFVHAEGANVGTEDARVVTSSA
                     NLYYNEQQLCWTKIYFGTIQAGGHATKDTMMSCTLPGGVDDHDIVIKFENVVVTP"
     gene            4099..>4338
                     /locus_tag="VFC43_05860"
     CDS             4099..>4338
                     /locus_tag="VFC43_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30743.1"
                     /translation="MPHDDWEQIHYQELPDWMKEGLKSDRRITKSNTRIFSGKPFNYK
                     VQYTMTGDIVSVSYWKSAPATPPPVIKKPDRRVLFV"
BASE COUNT         1147 a         1049 c         1070 g         1072 t
ORIGIN      
        1 taccaatacc cggtcgacgg ccggaagacg gcggtaccat ctttaccgtt cccgtcccag
       61 tcaccagtga ttgggatatc tcccggtgct ccgaaatgga tggatttatc gacagttcct
      121 gtattattgt aatccagata ccagttgcct actgagtttc ggaatattcc cactgcattg
      181 accggacata ttatgcagag aaaaagtagc aaaacaccca aaaacgtgac gatagatgtt
      241 tccttcaatt taattcactt cccatccaag aaaattacgg ttaaacaatg aaacaaaatt
      301 ggtaactggc aatatgaaga tgagcaaggc acctttgcat tgcactatcg atctatattt
      361 ttgctcgacg gtaaattaat aacctgagaa catacaaagc gtgcggggcg ggggtttcgc
      421 ggcatctcag gtttaatacc ggcgggttct ttcctgcaga gaaaacttta tggtctgata
      481 tgatgattac gcaaaagata aaagaggaga ctattctctt ttacgacggt tacataccgc
      541 aacctggaat aattcccggt gaagataaaa gagtagtgag ggaagaatta tggttacttt
      601 ttgtccaaag tgcgggactc aggcagtcga tggccaatcc cagttctgca atatgtgtgg
      661 ttcggagttg cccgctgata tatcagaaga aaccggaaaa tattgtccga attgcacagc
      721 aaaaattact gaccgggaag cagtctcctg tgccaggtgt ggttttcagc ttttgccaaa
      781 aaagccctta accccttcgg tcagaccaac aaggcaatgc ccacagtgtg gcaacccggt
      841 tgttgatgaa aacaggtttt attgcaattc atgcggcgca tatataaaga atacaaaggc
      901 aggaaacgtt tccctgtcag acggtcccgc cgggtcaaaa acaggaatta aaaaaccagt
      961 cattattccg gggattcatc agaatatgga aacaaagacg gttaccgaac ccgggtccgt
     1021 tgtcgcgaaa aggaactttg atggtaaaaa tccgattata aaaaagattg ggattgtgat
     1081 ggtcgtcatt gtggggatcg cactcctgac ctgggttggg tccatgctcc tgggatccgt
     1141 ttctctcctg ccatccgaca acgaggtact cgtaacacag gacctggatt caataacatt
     1201 acagctcagt gatcttccgt ccgggtggat gagtggtgat gccgtgggta ccgatgatgc
     1261 atattctgcc cagtttttta ccagttcgga gaacagcgag gccctggtag aagaaaccat
     1321 tacgcggttt cccgggattg aggaggccag gctggaacta aattctgaaa ggcaacaggt
     1381 aaccggtctt acagtcgaga ccataaatct tggaaacggg ggctttagct atattgacgt
     1441 gaattatgtc atggtcattt tccgcagggg aaatattatt gtaaaaattg aagatacccg
     1501 gactgaatac caggaaaatc caaccatgaa taacgcaaaa aactatgcag aaattgttgc
     1561 aggcaggatc aaataatcca cgggggaaca atattttctc gttgcctgca aaaaaatctt
     1621 catccgggaa attatccaaa aaaccctgac ttttctccgc aactcttatg tattctgtta
     1681 actcaaaccc tccgtcatgg gtatcctgat tgatgatccc cgtctccggg accagcgatg
     1741 tgttttttca gaccgtcatg atgccggcag gaaactggga gcactgatac aatcccgccc
     1801 ttttttagaa gacccgttcg tgatggcaat tccggccggg ggtgtccctg tcggtgttga
     1861 gatcgcacgg gccctgcatg cccctttcag ccttatggtc gtaagaaaga tccaggttcc
     1921 cggcaacacg gaagcagggt tcggtgccgt gacctttgac gggcatgtac tgatcaacga
     1981 acacctgcgt tcagtgctgg acctgtcagg agaggaagtc gacactgcaa ttgctgctac
     2041 ccgcaggaat gtcagggagc gggtggcccg ctatatgtct ggcaggccat ttccggaccc
     2101 ggcaaaaaag tgtgtggtcc tgaccgatga tggccttgca tcgggatata caatgctggc
     2161 cgctgttgag agcatccgta cctcacaccc ggaacggatc atcgtggccg ttcctacagc
     2221 ttctgcctct tcagccgggc gtattgcacg tgaggttgac gaatgcatct gcttaaacat
     2281 ccgcagtggg aaccgttttt ctgtagcgga ggcctaccgg aactggtacg atctggatga
     2341 ccgtgaagta cttgccgaac tccgggctta tcagggacaa acgtaatgaa agtcctgtta
     2401 aaccgccgtg cgatagtgcg tatttttttc aaaagaaaat tgtttaacca ccggatacac
     2461 tatcctgcac atgtgagaaa cctatgaacc gatcgcttct ctcttctgct atcttctgcc
     2521 tcctggtcct cgctgcattc gcggcaggat gcacaaacgc aaaacaggca aataccacac
     2581 cgttaccatc atctgtcacc ggcagcatga tctccataac cgctgaccaa aagacatacc
     2641 ctgcatatat ttccgtcccc acctttaccg gtaaacaccc ggggatcatc ctccttcatt
     2701 ccttcaatgg gctcgaacct ggctacaaag tgatgtgcga ccgtatggca actgatggat
     2761 tcgtcgtgat cgcccctgaa tggcagacgt ttgaccggca ggccggcgac ccggaggtcg
     2821 gggccgtggt ccgctcctca atagcagcac ttaaaacccg cgaggatgtc gatgcacaga
     2881 agctcggttt aatcggattc tgcgcctgga ggaggttcac gatgctcttc ttgccacagt
     2941 ttaaggactt cggggcgggt gttgcctttt atggcttccc gtactcccgg ggtaatacga
     3001 ataccacgac ccctgccggg catatcggtg agctaagcac gccgatactt gtgatacacg
     3061 gtttccggga ccaggccagt ccgataagcg ggatctataa ctacacaaag gaactggatg
     3121 ctgcggacaa gtactacgag ctcaaagtat accagggaaa acctcacggg ttcatgatag
     3181 tgaacggcac ctttggacag gatactgcgg caaaagatgc atattcggag atgaccggat
     3241 tcttccgccg catgctggaa taatcccttt ttttaatgac gctagtggaa ggagcaaaaa
     3301 aagaaattac ggggtaacga ctacattctc aaacttgatg acgatgtcgt ggtcgtcaac
     3361 cccgccaggc agtgtacagg acatcatcgt gtctttggtt gcatgtccgc cggcttgtat
     3421 tgttccgaaa tagatttttg tccagcagag ctgttgttca ttataataca ggtttgctga
     3481 cgaggtaacc acccgtgcat cctcagtacc gacattggca ccctcagcat ggacaaagag
     3541 gactatcggg tttgaaaaac tccttacggc agggtaggtc cagtatccgt cgacatggag
     3601 ttcaggggta tctttttttg caacggttgg tttgatcgtc ggggtggtag tcggggtgct
     3661 gacggaaacc ggtaccaggg aggttgatac aggcaccgta cctccagagg taaccgtgat
     3721 gattgaggac caggtattat acccccgttc ccgaatctca accgtatggt tccctgctgg
     3781 cactgccttt accgtaaccg gcgtagttcc atggtattca ctatcaaggt aagccccggc
     3841 accggaaggt gttgaggtga tctggatgac accggtcttt tcttcgggcg tggtggtgca
     3901 cccgcacaga aataccgaca gcaccagcag gcatatgaca atttttccta tcattgttcg
     3961 cacctgatac acggatacta acttgcatga tcttttccat aatatccttt ctgcttgaaa
     4021 atctgcagaa gatggatgtt tcagagtctg gttaaaagaa aatcctttta aacatccggc
     4081 agtgtactac tcaaaataat gccacatgac gattgggaac agatccatta ccaggagtta
     4141 cccgattgga tgaaagaagg gttaaaatct gaccgcagga tcacaaaatc caataccaga
     4201 atattttccg gaaaaccctt taattataaa gtccagtata ccatgaccgg cgatatcgta
     4261 tcggtatcct actggaaatc tgcccctgca acaccacccc cggtgataaa aaaaccggat
     4321 cggcgggtgc tctttgtg
//
LOCUS       DASRLV010000147         4345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_865626,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000147 DASRLV010000000
VERSION     DASRLV010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865626"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..1322)
                     /locus_tag="VFC43_05865"
     CDS             complement(126..1322)
                     /locus_tag="VFC43_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HZK30744.1"
                     /translation="MALDTGVTTWLMVSAILVMLMTPGVGFFYGGLVRKKNFISMIAL
                     SFVVLALVSIHWVLIGYSLAFGPDIGGIIGNLDFIGLNGVSADAGSGTYPPLLFMVFQ
                     LFFAAVTVTIVTSAVAERIKFSSFIVFVLVWLTIVYCPLAHWAWGGGWAQRLGLIDFA
                     GGTVVEICSGFGALALALVIKNRAGFGEYALEPHNIPMALLGAALLWFGWFGFNAGSA
                     LGVNSVAVSAFIATNTAAAAGALAWMFASWYHGKPSSLGMVSGAIAGMVAITPAAGFV
                     TPMVSMLIGTVAGVLCYGMMLLRVRKQCDESLDAWAIHGMGGLWGTIATGIFAAAAIG
                     GISGLAEGNSHQFVVNTVCAVAALIFSFVMTYCIGFVIDRTIGMRVTEDEEYVGLDIC
                     QHGERA"
     gene            complement(1547..2872)
                     /locus_tag="VFC43_05870"
     CDS             complement(1547..2872)
                     /locus_tag="VFC43_05870"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HZK30745.1"
                     /translation="MSADVTKLLKKIEADKVKFIRLQFTDIQGQPKNVSIQVIQAEKA
                     LTEGIWFDGSSVEGFARIEESDMMLKPDPSTYVILPWRPQEGKVARFICDVQTYGNKP
                     FDGDPRFILRKTIADAAKMGFTFNTGPELEFFLFKMYEGIPTTEFEDHGGYFDLAPTD
                     SAEDVRRDVDLALGEMGFEIEASHHEVADSQHEIDFKYGDALVTADRVITFKFATKSI
                     ALQYGLHASFMAKPIAGINGSGMHTHGSLAKNGKNAFYDPNGENQLSDTAMYYIGGIL
                     KHAKAITRVANPTINSYKRLVPGYEAPCYISWSAANRSALVRVPAARGNSTRAEFRSP
                     DPMCNPYLTFACMLAAGLDGIRNKIMPPESTNTNIYHLDEKARKRLHIDMLPGSLMES
                     NAALLKDEVLCKTLGEHVVSNLSSIAQMETDSFRLAVHPWELDRYLATY"
     gene            2994..4046
                     /locus_tag="VFC43_05875"
     CDS             2994..4046
                     /locus_tag="VFC43_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase family protein"
                     /protein_id="HZK30746.1"
                     /translation="MCGIIGVLDKRRQSVDGAKIKEALSMMNERGSGEGAGYVAYGIY
                     PEYKDYFALHVFFDNIRENKTALDLVLEKWGTIVHDEQIKTYEQPNIRKVHTPWRYFF
                     RPDRSLMPKSTTPDEDIVTHLVMKINAEKNGALIFSSGKNLGVFKAAGWPEDVANFYR
                     IEDYKGYLWLAHNRYPTNTPGWWGGAHPFNLLNWSVVHNGEITSYGTNRRYIESYGYK
                     CTMYTDTEVVAYLVDLLIRKHGLSERMAVRALAPPFWEDIDRMEAKEQELNRAIRLAY
                     GSALMNGPFAICVANENALVGFTDRIKLRPLVTGELGDRLFISSEEAAIRRMEPDVET
                     IAMPKAGEPVIGRVIA"
     gene            4043..>4345
                     /locus_tag="VFC43_05880"
     CDS             4043..>4345
                     /locus_tag="VFC43_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZK30747.1"
                     /translation="MTIGSIPLRYKVEIDPDQCMLCGRCVENCSYGVFRKDGDVIGVN
                     SRNCVACHRCSAFCPRDAISIYEKPTDYRSHPVWTREVRENIFNQAKTGKIVLAGMG"
BASE COUNT         1172 a         1055 c         1046 g         1072 t
ORIGIN      
        1 atcaatcgtt gagttaccgt atgttggaag gaagttgtat tcctatattt aaaaatttaa
       61 ctgtatattc attttttaat agtctgggac gcggtcaatg aactttaggg aaaaaaagaa
      121 aaatatcagg cacgttcacc atgctggcag atatccaggc caacatactc ttcatcttcg
      181 gtgactcgca tgccgattgt acggtcgata acaaacccga tacagtaggt cattacaaaa
      241 gaaaatatca gggctgcaac agcacatact gtatttacta cgaattggtg cgaattacct
      301 tcggcaagtc ctgatattcc cccaatagct gcagccgcaa aaattcctgt tgcgatagtc
      361 ccccagagcc cacccatccc atggatggcc catgcatcga ggctttcatc acattgtttc
      421 ctgacccgca acaacatcat cccgtaacag aggactccgg cgacggttcc gatgagcatt
      481 gataccatgg gagttacaaa gcccgccgcc ggggttattg ctaccattcc ggcaattgca
      541 ccgcttacca tgccaagtga actgggtttg ccgtgatacc agcttgcaaa catccaggca
      601 agtgcaccag cagcggctgc agtatttgtt gcaatgaacg cactgacggc aacactgttg
      661 actcccagtg cgcttcctgc attaaatcca aaccagccaa accataaaag agccgctccc
      721 agcagtgcca tcggaatgtt atggggttcc agagcatatt cgccaaatcc tgccctattt
      781 tttatcacca gcgcgagtgc aagagcacca aaacctgaac agatctcaac aactgttccc
      841 cccgcaaaat cgataagccc gagacgttgt gcccaacccc cgccccaggc ccagtgagca
      901 agcggacaat atacgatggt gagccatacg agaacaaaaa ctataaacga tgagaacttt
      961 atgcgttcag ctacggcgga tgtaactatc gtaacggtca ccgcagcaaa aaagagctgg
     1021 aacaccataa aaaggagcgg tggatatgtt cctgaccctg catctgcgct gaccccatta
     1081 agcccgataa aatccaggtt gccgatgatt cccccgatat caggaccaaa agcaagtgaa
     1141 tacccgatca aaacccagtg gatacttaca agcgcaagca ccacaaatga gagggcgatc
     1201 atagaaatga aattcttctt ccttaccaat ccgccataaa aaaacccaac gcccggggtc
     1261 atcagcatca caagtattgc tgagaccata agccaggttg taacaccggt atccaaagcc
     1321 atacttttca cctttgcagt tcgcttatac tgccgatgcc ctggtaacgg aaattattga
     1381 ttattctgga gataaacagg tttttgagga tactaaattg aatggaccct atagtcatct
     1441 cacccagtat gcatgctatt ccgcttgaag aacatcgaca gtaagttgcc ggattacaag
     1501 tccgaacatt gccgacaaaa aattttttta gaaatttgaa aaatttttag tatgttgcca
     1561 ggtacctgtc aagttcccat gggtgaactg caagccggaa cgaatcggtt tccatctgtg
     1621 caatgctcga aaggttcgag acaacatgtt cgccaagagt tttgcagagt acttcgtctt
     1681 tgaggagtgc tgcgttggac tccataaggc tgccgggaag catgtcgata tggagtcttt
     1741 tccgggcttt ctcatcgaga tgatagatat tcgtattcgt gctttcaggt ggcataatct
     1801 tgttcctgat accgtcgagt cctgctgcga gcatacaggc aaaggtgaga taggggttgc
     1861 acatcgggtc cgggctgcgg aactcagcac gagtgctgtt accccgggct gccggaacgc
     1921 gaacaagcgc tgaacgattg gctgcgctcc aggaaatata acagggggct tcgtaacctg
     1981 ggacaagacg cttgtatgag ttgatggtcg gatttgcaac acgtgtaata gctttggcgt
     2041 gtttcagaat accccctata taatacattg cggtatcaga gagctgattc tcgccattgg
     2101 gatcatagaa agcgttttta ccattctttg caagtgaccc gtgcgtatgc attccgctgc
     2161 cattgatgcc tgcaataggt tttgccataa agctggcgtg caaaccgtac tggagagcga
     2221 tcgatttggt ggcaaacttg aaggtaatta cccggtccgc cgtgacaagg gcatcgccat
     2281 atttgaagtc tatttcgtgc tgactgtccg caacttcgtg gtgagaggct tcaatctcaa
     2341 atcccatctc gcccagagca aggtcaacat cccttctgac atcctctgca gagtctgtcg
     2401 gggcgagatc gaaatacccg ccgtggtctt caaattctgt agtcggaatg ccctcataca
     2461 ttttaaacag gaaaaattca agttcgggac cggtgttaaa agtgaatccc atctttgcag
     2521 cgtccgcaat ggtttttcgc agaataaaac ggggatcacc atcaaatggc ttattaccgt
     2581 atgtctggac atcacagata aaccgggcaa ccttaccctc ctggggtctc cagggaagga
     2641 tcacgtacgt tgaagggtcc ggcttgagca tcatgtccga ttcttcaata cgggcaaatc
     2701 cttcgacaga tgacccatca aaccagatgc cctcagtcag tgctttctct gcctgaataa
     2761 cctggatcga gacatttttc ggctgaccct ggatgtcagt aaactgcagg cggatgaatt
     2821 ttactttatc cgcctcgatt ttttttaaga gtttggttac atctgctgac atattggaag
     2881 acatgttcca cccatttgta tttatactta ttcaactaat ctgtctgaac gaatgttcag
     2941 tagtgcaatg aacatatgtt catgaataca agatcattct gtactgtgat aatatgtgtg
     3001 gtatcatagg tgtgttggat aagcgccgcc agagtgtgga tggtgccaaa atcaaagagg
     3061 cgctctccat gatgaatgag cggggaagcg gtgaaggggc cgggtatgta gcgtacggta
     3121 tatatcctga atataaggat tactttgcac tccatgtttt ctttgacaat atccgtgaaa
     3181 acaagaccgc acttgatttg gtgctcgaga aatggggaac tattgtccat gatgagcaaa
     3241 tcaagacgta tgagcagccc aatatccgga aagtacatac tccctggcgt tattttttcc
     3301 ggcccgacag gagccttatg ccaaaaagta ccacaccaga tgaagatatt gttacacacc
     3361 tggtcatgaa aatcaacgcg gaaaagaatg gtgctctcat cttctcatcc ggtaaaaatc
     3421 tgggcgtatt caaagctgcc ggctggcctg aggacgttgc aaatttctac cggatcgaag
     3481 attataaggg atatctatgg cttgcacata accggtaccc gaccaatacc cctggctggt
     3541 ggggcggtgc acatcctttt aatctcctga actggagtgt tgtccataac ggggaaatta
     3601 cctcgtatgg cacaaaccgg cgctatatcg aaagttatgg atataaatgc accatgtata
     3661 ccgataccga agtggtagcg tatcttgtgg acttgctgat acggaaacac gggctctcgg
     3721 aaaggatggc agtgagggca ctcgcgcccc cgttctggga agacatcgac cggatggaag
     3781 ctaaagagca ggaactcaac cgggcgattc gtcttgcgta tggttcagcg ctcatgaacg
     3841 gcccatttgc aatctgtgtc gcaaacgaaa atgcgctggt gggctttact gatcggatca
     3901 agcttcgccc gctcgtgacc ggagaactgg gagatcgact ctttatttcc agcgaggagg
     3961 ccgcaatccg ccggatggaa ccggatgtcg aaaccattgc catgccaaaa gcgggtgaac
     4021 ctgttatcgg gagggtcatt gcatgaccat aggcagtatc ccccttcgct ataaagtaga
     4081 aatcgacccg gaccagtgca tgctctgcgg tcgctgcgtg gaaaactgct catacggggt
     4141 gttccggaaa gacggggatg taatcggagt caactcccgc aattgtgttg cctgtcaccg
     4201 ttgttcggca ttctgccccc gtgatgcaat cagcatctat gaaaagccca cggattaccg
     4261 gagtcaccct gtctggacgc gtgaggtccg ggaaaatatc tttaaccagg caaagaccgg
     4321 caagatcgtt cttgccggta tgggt
//
LOCUS       DASRLV010000148         6554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_298102,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000148 DASRLV010000000
VERSION     DASRLV010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6554
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298102"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1154)
                     /locus_tag="VFC43_05885"
     CDS             complement(48..1154)
                     /locus_tag="VFC43_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF373 family protein"
                     /protein_id="HZK30748.1"
                     /translation="MLSVDRDDDIGWKAKIESPAIGRAACLNAANTLALADPEDSDVN
                     AIFSAVKIYDELTAKGEETAIALVAGNHLHMIEGDRRIAASLEQVINETQATNCILVT
                     DGAEDEYVIPIIQSKIPVSSIRRVIVNQMPNLEGTYYILKKFLDDPKVSRMVFIPIGL
                     AMMLYATAYLLDYPGIATIIVVGVIGFYLLYKGFGFDEIVHGIIDALRISLSRGRFSF
                     VTYTTTILLIIIGFTNGFLTVLKYYSPDFGIIHYLMLFIFGGIGWLIAAGLVMSIGII
                     IDVYMNERENLGKVIVFPFFITAIGFILYCASLSLLPVKDIPNFPITPDGVAIQVLYF
                     AVFGILSAIIGMIIQFFVNKKIAELRKQEIIEVM"
     gene            complement(1232..2092)
                     /locus_tag="VFC43_05890"
     CDS             complement(1232..2092)
                     /locus_tag="VFC43_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HZK30749.1"
                     /translation="MLNDLMEKRKKILAESEEHKNRRNELNASASKFARERNTLNNQT
                     REFVEEAQKNKDLRDKYNQDVLDLKAQRNDFNDKANVLFEEIEEFKKEHGTLKNRGIK
                     ELQKQIEHMEYRQQTEVFTTDKERELIEKIKQMMAQVREQEAELEQNKEMRTKITEAR
                     DFRKLASDLHAKVTEFAELAQKHHDLMVESYRKADKSREAADASHKSFVESQESADSE
                     HKFFITCQKELRDYDKVISGLRKKTKKVKVTKEQKAVRKEAESLFKNFRAGEKLTTDD
                     ILLLQRSKLI"
     gene            complement(2670..3836)
                     /gene="ftsZ"
                     /locus_tag="VFC43_05895"
     CDS             complement(2670..3836)
                     /gene="ftsZ"
                     /locus_tag="VFC43_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HZK30750.1"
                     /translation="MQSIINDALKNAELEKELNKPGSANAMEEDFVGQPRIVIVGCGG
                     AGNNTVNRIHHMGVSGAETIAINTDKQHLDMIQADKRILIGKSLTKGLGAGGYPDVGK
                     RAAEMARPTLEAVLESADLVFITAGMGGGTGTGSAPVVASIAKEQGAIVVGMVSYPFQ
                     VEKARLIRAEEGLEALASASDSVIVLDNNRLKNFVPNLPLGQAFSVMDQLIGETVKGI
                     SETITEPSLINIDYADVRAIMSKGGVAVMLVGESKQQNKAESVVRECLSNPMLDIDYR
                     GATGSLIHITGGTDLTLQDAEEVATSLTYELDPHADVIWGARVRNDMEGKIRVLAIMT
                     GVKSAQILGTRQSYKTMIADIENKRSNPKAVEMPQNRKTSRDQPSGGGPIDWVG"
     gene            complement(3847..4026)
                     /locus_tag="VFC43_05900"
     CDS             complement(3847..4026)
                     /locus_tag="VFC43_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HZK30751.1"
                     /translation="MMQRITLRLPEQQITLLEQMVEAGEFPNVSEAVRAAVRELVEKR
                     SNRVLKDIDQVSFKV"
     gene            4225..5223
                     /locus_tag="VFC43_05905"
     CDS             4225..5223
                     /locus_tag="VFC43_05905"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZK30752.1"
                     /translation="MVFVFPKREVHGGTGKREREKSKKNLVDFSASTNPFPPRFSWNC
                     DPSCLAYYPDDTYFELKKRISQTFHRPFEEICVGNGSIEIIRVFCQVALKGTKKTFFT
                     DTPTFGEYELSARLSGAKRTNSDKADVAFICNPNNPTGILHRKQDLLQTLKKVKSHEG
                     LLFCDEAFIELSDPSQTLADVIDPNLFVLHSLTKSFAIPGIRFGYGFGDPSLIDKIEI
                     SRPPWSVNAFAEAFALEAFQHMDQLSQSRAAIATERERLATGITALGLHCHPSSVNFI
                     LVDCGRDVTPLCTALLRHDILVRNCTSFGLPSCIRVAVRTPEENRLLLEALSACTH"
     gene            5211..5828
                     /locus_tag="VFC43_05910"
     CDS             5211..5828
                     /locus_tag="VFC43_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HZK30753.1"
                     /translation="MHALIMAGGEGSRIHLGEKPLVLVCGRPMIAYITDAFTGAGIDP
                     VVAASPKTPMTMNWCRAQGIDVVRSEGRGYIYDMIEAVKALDDQRPLFICVSDIPCIT
                     AENIQAIYEGYRVSGKDACSTWVPVQLVQSFGSTITYQEQVYGVGACPSGLNILRGDL
                     IDQPQDELRMVLNEPGLALNVNTREDLAIAEEFLKHKSRAKSLGI"
     gene            5944..>6554
                     /locus_tag="VFC43_05915"
     CDS             5944..>6554
                     /locus_tag="VFC43_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HZK30754.1"
                     /translation="MVESQEIELAGHIIDSGIMTQLFDRVMDMGGNFEILVFDIGKKK
                     TDPSYARLRIKASNKEKLLSILSELHRLGARPVEVKDVYLVPAESDRVVPKGFYTTSN
                     HPTQVKYNGEWIPVESIEMDFLIVVDPVAKHALSVALGKIRKGDLVVVGEQGVSVSYP
                     ERPRESSTFEFMHGTVSPERPSETLIAQIAREILDIRKQGGKIA"
BASE COUNT         1650 a         1602 c         1499 g         1803 t
ORIGIN      
        1 catccaggaa cgcgggcagc cgacttaaaa tgacgggtaa agaaatttta catgacttca
       61 ataatttcct gcttgcgaag ctcggcaatt ttcttattga caaagaactg gatgatcatg
      121 ccaataattg cagaaagtat accaaagacc gcaaaataga gtacctgtat tgctacaccg
      181 tcaggtgtaa tgggaaaatt cgggatatct tttaccggaa ggagagaaag acttgcgcag
      241 tagagaatga acccgattgc cgtgataaag aagggaaaga cgatcacttt gcccaggttc
      301 tcacgttcat tcatgtacac gtctataata atgcctatag acattaccag gcctgctgca
      361 atcagccagc ctattcctcc aaaaataaat aacataagat aatggattat tccaaaatcc
      421 ggcgagtagt atttcaatac ggtcagaaaa ccgttggtga atccgatgat gatgagaagg
      481 attgttgtag tatatgttac aaacgagaac cgcccgcggg aaagtgagat ccggagagcg
      541 tcaataatcc cgtggacgat ttcatcaaac ccgaatcctt tatagagcag ataaaatccg
      601 attaccccta caacaataat tgtagctata cccgggtaat cgagcagata tgcggtggca
      661 tagagcatca tcgcaagacc gatgggtata aaaaccatcc gggaaacttt cgggtcatca
      721 aggaattttt tcagaatata gtacgtgcct tcaagatttg gcatctggtt tactattacc
      781 cgccggatac tggaaaccgg gatcttagac tggatgatgg gaatgacata ctcatcctcg
      841 gccccatctg tcacaaggat acagttagtc gcctgggttt cattgataac ctgctcaagg
      901 gatgctgcaa tcctgcgatc accttcaatc atatggaggt gatttcccgc tacaagggca
      961 atcgccgtct cttcaccttt tgcagtcagt tcatcgtaaa tttttactgc cgaaaatata
     1021 gcattgacat cagaatcttc aggatccgca agtgctaggg tatttgctgc gttgagacag
     1081 gctgcacgac cgatggcagg actttcgatc tttgccttcc agccgatatc atcgtcccga
     1141 tcaacactta acactaaagt acgacccaga gacattatta gtataaaatt gcggtgaacc
     1201 tattaaatta ttactgacgg taaaaaaggt attagatgag ttttgagcgc tggaggagca
     1261 atatatcatc agtggtgagc ttttctcctg ccctgaagtt cttaaagaga ctctctgctt
     1321 cttttcgaac cgctttttgt tcttttgtca ccttaacctt ctttgtcttc ttgcgcaggc
     1381 cggaaatgac cttgtcgtaa tcgcgaagtt ctttctggca ggtgataaag aacttgtgtt
     1441 cggagtcagc tgattcctgc gattcgacaa aactcttgtg cgaagcatct gcggcttcgc
     1501 gtgatttatc cgcctttcta taggattcga ccatgaggtc gtggtgtttc tgggccagtt
     1561 cagcaaactc ggtcactttt gcatgaaggt cagatgcgag tttccgaaaa tcgcgggctt
     1621 cggtaatttt tgtacgcatc tccttattct gctcgagctc cgcttcctgc tcccgcacct
     1681 gtgccatcat ctgtttgatc ttttcgatga gttcccgttc tttgtccgtt gtaaacactt
     1741 cggtctgctg ccggtactcc atgtgctcaa tctgcttctg gagttccttg attccccggt
     1801 tcttcagcgt tccgtgctct tttttgaact cttcgatctc ttcaaacaga acatttgcct
     1861 tgtcattgaa atcattgcgc tgcgccttaa gatcaaggac gtcctggttg tatttatccc
     1921 ggagatcctt gtttttttgc gcttcttcca cgaattcccg cgtctgattg ttaagggtat
     1981 ttcgttcacg ggcgaacttg cttgcagagg cattgagttc gttacgtcgg tttttgtgtt
     2041 cttcagactc ggcgagaatc ttctttcgtt tttccatcag gtcattcaac atgcgttacc
     2101 actcctcatc gattccccgg cttgggaagc agcagttgca gggtggaaag aaaaacatct
     2161 aagaagatct caacatggac tcttttgatt ctctcacatt acaactctgc atgcaggatg
     2221 cagggcgaaa aacatcagaa aaggatacca gcgcagaatt cagataagac cccaaaacga
     2281 agtctttgtt caacactctg ttcatgatgt tcaactcccg ataccaggac tcaccagatc
     2341 acgtgatgtc gtccccattc tcccagcaag ggataggccg aatgttctat attttaaagg
     2401 cggggaggat tattaagcgt ttcgccgaca cccccgaaga taatattttt aatagtatgc
     2461 cggagggtaa ttttccgatt aaattccggt aaaaacagtg caaaaagggg tttttcataa
     2521 ggagtaccct ttactccttt tttactggtc tgatttgcca atccagtgtg gcttatttcc
     2581 ggtttaaccg ggcaattcct ggaattttct ccagaggctt tgtttttgga acgtttgaat
     2641 ttttttcctt tgcgtggttt tttgtgtaat taaccgaccc agtcaatcgg gccgccaccg
     2701 cttggctggt cccggctggt tttccggttc tgtggcatct cgactgcttt tggattcgag
     2761 cgcttgtttt cgatatcagc gatcatagtc ttgtacgact ggcgggtgcc gagaatctgc
     2821 gcgctcttta cgccggtcat gattgccagc acacggatct ttccttccat atcattgcgg
     2881 acacgggctc cccagattac atctgcatgg ggatcaagct cgtaggtaag cgaagttgct
     2941 acttcttctg catcctgcag ggtaaggtca gtgccacccg taatatggat caggcttccg
     3001 gttgcaccgc ggtaatcgat atcaagcatc gggttgctca ggcattcacg tacaacgctt
     3061 tcagctttgt tctgctgttt gctttcaccg acgagcatga ctgcaacacc gccctttgac
     3121 atgatagccc ggacatcggc gtaatcaata ttgatgagcg atggttctgt gattgtttca
     3181 gaaatgcctt tgacagtctc tccaatgagc tggtccataa cggaaaatgc ctggcccagc
     3241 ggcaggttcg ggacgaaatt cttcaaccgg ttattgtcaa gaacaattac tgaatctgaa
     3301 gctgatgcaa gtgcttcgag accttcttct gcacgaatca ggcgggcttt ttcaacctgg
     3361 aacgggtaac ttaccatgcc gacgacaatt gcaccctgct cctttgctat cgatgcaact
     3421 accggtgcgg aaccggtacc ggtacctcct cccatacctg ctgtgatgaa aaccagatcc
     3481 gccgattcaa ggacagcttc aagtgtcgga cgagccatct cggccgcgcg cttcccgaca
     3541 tccggatatc caccggcacc gagtcctttt gtgagggact tgccaatgag tatgcgtttg
     3601 tccgcctgga tcatatccag gtgctgcttg tcagtgttga tggcgatcgt ttctgcaccg
     3661 gaaacaccca tgtggtggat acggttcacg gtattgttgc cagcaccacc gcaaccaact
     3721 atcacgattc ttggctgtcc gacaaaatct tcttccattg catttgctga acctggtttg
     3781 ttcagctctt tttcaagttc ggcgtttttc aacgcatcat taattatact ctgcatactt
     3841 accctctcat accttgaatg aaacctggtc gatatctttt aagacacggt tagaacgctt
     3901 ttcaacaagt tcacggaccg ctgcccggac agcttctgat acattgggaa attcccctgc
     3961 ctccaccatc tgctcaagca gagtgatttg ctgttcgggt aaccggagcg tgattctttg
     4021 catcatgatt gtatctcaca tttctgacat atgtctgtca tctgtcattc atatgtcaga
     4081 caaagagaac ctttgtgtct gacttaacca gatgatccaa aattaatcat aaatgtataa
     4141 aaaccttctt ctttgaaacc aaggccatct caaaacatat tttagacagc caatcaaagt
     4201 atttcaggaa aaacaggttt atttatggta tttgtcttcc ccaaacgcga ggtccatggc
     4261 ggtactggca aaagggagag agagaaatcc aaaaaaaacc ttgtagattt cagcgcgagc
     4321 accaacccgt ttcccccccg cttcagctgg aactgtgatc cctcatgcct tgcatattac
     4381 cctgacgata cctatttcga actcaagaaa aggatttcac aaacgttcca ccggcctttt
     4441 gaggaaattt gtgtggggaa tggatcgatc gagatcatca gggtcttctg ccaggtcgcg
     4501 cttaaaggga ctaaaaaaac cttttttacc gatacaccaa cctttgggga gtacgaactt
     4561 tctgcccgtc tttccggagc aaaacgtaca aattcagaca aagcggacgt tgcgtttatc
     4621 tgcaatccga ataacccgac cggtatcctg cacagaaaac aggatcttct ccagacacta
     4681 aaaaaagtaa aatctcacga aggcctgctg ttctgcgatg aggcttttat agaattgtca
     4741 gatccctcac agaccctggc tgacgtgatc gacccgaatc tttttgtcct ccattcactt
     4801 accaaaagtt ttgcaattcc cggcattcgt ttcgggtacg gttttggaga tccgtcactt
     4861 attgataaaa ttgagatatc aagaccccca tggagtgtca atgcctttgc tgaagcgttt
     4921 gctcttgaag cattccaaca tatggatcaa ctttcccaat cacgtgcagc tattgcaacg
     4981 gaacgggaaa ggctcgcaac cggaattacc gccctcggac tgcactgcca cccatcgtcg
     5041 gtaaatttta ttctcgtgga ttgcggcagg gacgttacac ctctctgtac cgcactcctc
     5101 cgccacgaca ttcttgtccg caactgcacc tcgttcgggc tgccctcgtg tatacgtgtg
     5161 gctgtcagga ctcctgagga aaaccggctt cttctcgagg cactctctgc atgcacgcac
     5221 tgattatggc agggggtgag ggaagcagga tccatcttgg agaaaaaccc ttggttttag
     5281 tctgtgggcg ccccatgatc gcatatatta ccgatgcttt caccggtgcc gggatagacc
     5341 cggtggttgc ggcatcccca aagaccccta tgaccatgaa ctggtgccgg gcacagggca
     5401 tagatgttgt caggtcagaa ggaagagggt atatttacga tatgatcgag gcagtaaagg
     5461 ccctggatga tcagcgcccg ctctttatat gcgtatccga tattccctgc ataaccgctg
     5521 aaaacattca agcgatttat gagggctacc gcgtgtccgg aaaagatgcc tgctcgacat
     5581 gggtaccggt acagttagtc caatctttcg ggtccaccat cacgtatcag gaacaagtat
     5641 acggtgttgg tgcatgtccg tccgggctca acattctgcg tggcgatctt attgaccaac
     5701 cccaggatga actccggatg gtgctaaacg aacccgggct cgccctgaat gtaaacaccc
     5761 gggaggatct tgcaatcgct gaggagttct taaaacacaa atcccgggca aagtctcttg
     5821 gaatttaaca cggttacaga tatcaaacat cccaattttg tgccccagca ctcaaaacaa
     5881 gcgtttttca gtcatatttg ggaggaatgc atattaaggg gcttcaccca agatctctgt
     5941 attatggtgg agtcgcagga gatcgagctg gcaggtcata ttatcgattc cggtatcatg
     6001 acgcagctct tcgacagagt catggatatg gggggaaatt tcgagatcct tgtcttcgac
     6061 atcgggaaaa agaaaacaga tccgagttat gcccgcctgc gaataaaggc atccaacaaa
     6121 gaaaaactcc tctccatcct ctcagaactc caccgtcttg gtgcccgtcc ggtggaagta
     6181 aaagatgtat atcttgttcc cgcagaatct gaccgtgttg tgccaaaagg attctataca
     6241 accagtaatc atccgacaca ggttaaatat aatggtgaat ggatcccggt agaatccatt
     6301 gaaatggact tccttatcgt ggttgatccg gtagcaaagc atgcactttc tgtcgcactt
     6361 ggtaaaatac ggaaaggcga cctcgtggtt gtcggggagc agggtgtcag cgtcagttat
     6421 cctgagcgtc cccgtgagag cagtaccttt gagttcatgc atggcacagt atcgccggaa
     6481 cgaccgagcg agactctgat tgcccagatc gcacgggaga tacttgatat ccgtaaacag
     6541 ggaggtaaga ttgc
//
LOCUS       DASRLV010000149         5386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_542092,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000149 DASRLV010000000
VERSION     DASRLV010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5386
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542092"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..624
                     /locus_tag="VFC43_05920"
     CDS             16..624
                     /locus_tag="VFC43_05920"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZK30755.1"
                     /translation="MIEYGVNTIGKGSQIFEPVTLGFPSRDKVGQTGFAGTTIGVNSV
                     IRSGTIIYCDVSIGDNFQSGHNVMIREKTTIGNRVAIGTSAVIEGTTIIGDDVSLQSM
                     VYIPTNTVIGNKVFIGPNTVLTNDRYPPTGIGGLKGPQIRDGAAIGANATLLPGVCIG
                     EGALVAAGAIVTRDVPAHMLAIGAPAKITDLPQKMVRDGHKK"
     gene            909..3503
                     /locus_tag="VFC43_05925"
     CDS             909..3503
                     /locus_tag="VFC43_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006487 - protein N-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharyl transferase, archaeosortase A
                     system-associated"
                     /protein_id="HZK30756.1"
                     /translation="MVSFDFKNRRTQLIIGLVALFCAFALWLRLLPMLNMGNTDVLMM
                     VASDDPLYNLRQVEQILANFPNYAWYDPMSLFPTGMSVPWGSLFPTIIAVFCLITGAA
                     TRPEIISTGLLIPPLMGVMIVAIMYFVGKNCGDWKTGLLASGFTAIVSGQFFYRSFYG
                     YMDHHIAEVLFSTIFCLLYMATVLSEKDLKIDLKNLNSYKKTLLLSGLTGIAYLLGLF
                     VMPTMVLFAMIVGIFTIIQFVIDVIRGKTSEYLLVINTVIFSIAIIGLLAYGLKDPGV
                     NLIFYSIGHVYAYLALIGGTGFLYGVARFVKEKDWYYYPAALIGSGIVFSLILFIASP
                     QLYNLFISSLFSFFGQQAVTNTVQEARGWAPDLAWFTFNYGIILMAGGALVMLYNNLK
                     DEHPHQIFALVWSLIMLISTWQHIRYEYYLAVNVALLSAVCVSFTIDYAWTDIRRLAT
                     RISSDKDVPEFKKEVKEETSKGKKQKKTIKKSSDTSMSGLLALVFVVLAVGLGILFAY
                     TSASISYSNAAGNTIRMNPDWRETLDWMGNNTPDTGVNYTTIYDPKTFQYPPQAYGVM
                     SWWDYGHMITYIAKRIPNANPFQAGVTGPGGSANFFMSISEDEGNQVLDHAKTRYIIT
                     DIEMDTGKFWAMSTWYNTSEGAAPYQMTLLAPAQNNLNSYESVLLNTRLYYFTMISRL
                     HNFDGSLKTPTSAYYVEYADPSISQVSLPVITAAEAVNATDAVSRAADYNLRASAGYH
                     ASALSPAITLPIETVPALRHYRLVHESPSNVFNSNTTDVKYVKVFEYVKGAHIQGEGI
                     IEVPVITNTGRTFTYQQASVNGEFIVPYSTTGNPYGVKTTAKYRVVSSGRTYEVPESA
                     IMDGALVR"
     gene            3578..4552
                     /locus_tag="VFC43_05930"
     CDS             3578..4552
                     /locus_tag="VFC43_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HZK30757.1"
                     /translation="MVRRSVIYGEIFTHHDMENHDESAARLEAALEGVPEGVPVIAPE
                     CAPESDLARVHEHSYIHMIREFSSHGGQHFLDPSTYVDAETYTVASFAAGAATEAVNR
                     SIDGEACFAIIRPPGHHAEPDRAMGFCIFNNAAIAAAVALDRVDKVAIIDWDLHHGNG
                     TQRIFSTNDHVLFCSIHQGNVFPYTGWVDEIGTGRGKGFTINAPLRAGSTIADYRLVF
                     EEVFIPALEQFRPDAVIISAGQDALFDDPKGGMMLFPADYGTLTGILRDATDNPLALV
                     LEGGYGPSHGEAVRQIFNSLHGSPVPPCQSEPHRTTRDIIAALKKIRM"
     gene            4609..4902
                     /locus_tag="VFC43_05935"
     CDS             4609..4902
                     /locus_tag="VFC43_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2098 domain-containing protein"
                     /protein_id="HZK30758.1"
                     /translation="MSAEEITLGMRVRYPRTGTTGTILRIEQVRGEAFAELDTTNLLY
                     RIDYLIPATGPEKSTKTVIDDAKKVIEREKEFVSGRELQDAWKATDQSCEGGG"
     gene            5266..>5386
                     /locus_tag="VFC43_05940"
     CDS             5266..>5386
                     /locus_tag="VFC43_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZK30759.1"
                     /translation="MHDVRVPSVNIGVVGHVDHGKTTLVNALTGTWTDRHSEEV"
BASE COUNT         1424 a         1258 c         1243 g         1461 t
ORIGIN      
        1 gagcgtgact gttgaatgat tgaatatggt gtaaatacta ttggcaaggg ttcgcagatt
       61 ttcgaacctg taacgctggg atttccttcc cgggacaaag tgggacaaac cggcttcgcc
      121 ggtacaacca tcggtgtaaa ttccgtgatc aggtctggca caattatcta ttgcgatgta
      181 tctatcggtg acaattttca gagcggacat aatgtaatga tccgtgaaaa aaccacgatt
      241 ggtaaccggg tggcgattgg tacctctgct gttatcgaag gcaccacgat catcggggat
      301 gatgtaagcc tccagagcat ggtatacatt ccgaccaaca ccgtgattgg gaacaaggtc
      361 tttatcgggc cgaatactgt gcttaccaac gatcgttacc caccgacagg tattggggga
      421 ttgaaagggc cccagatccg tgacggtgca gcaattggcg ccaatgcaac ccttttaccc
      481 ggtgtctgta ttggtgaggg tgcactggtt gcagctggtg caatcgtcac ccgtgatgtc
      541 cctgcacaca tgctggcaat cggtgctcct gcaaaaatta ccgatctgcc acaaaagatg
      601 gtccgggatg ggcacaaaaa ataatatctg tgtccttttc catttcctgt ttttttcctg
      661 tagctgtcag gaaagcaatt attaccatgg atatttcttg gggcatgtag ataataccgt
      721 cattcattgg ccggactcgg gcaaatgtcc tgccttctct atctgcaatc ctttttcaga
      781 attaaaaaaa ctatcatggg aaaatatctc caatacatgg attttttcca gagtctcact
      841 ttgaagaaat caaccaaatt tattaatggt ctcaacggta aaccaagtac ccaagaaagg
      901 ttgatttcat ggtaagtttt gattttaaaa accgccggac ccaactgatc atcggcctgg
      961 ttgcgctatt ttgtgccttt gccctgtggc ttcgtttgct tcctatgctc aatatgggaa
     1021 atactgatgt cctgatgatg gttgccagcg acgatccgct atacaacctg cgacaggttg
     1081 aacagatact ggcaaacttc ccgaactatg catggtatga tcccatgtca ttatttccga
     1141 cagggatgtc agttccctgg gggtcccttt ttccgaccat catcgccgta ttctgcctga
     1201 ttaccggtgc tgccacccga ccggagatca tcagtacagg acttctgatt ccacccctga
     1261 tgggagtcat gatcgtggca atcatgtact ttgtcggtaa aaactgtggt gactggaaaa
     1321 caggtctttt agcttcagga tttactgcca tcgtatccgg ccagttcttt taccgctcat
     1381 tttatggata tatggaccat cacattgcag aggttctttt ttccaccatc ttctgcctgc
     1441 tgtatatggc aacggtcctt tcagaaaaag acttaaaaat agacttaaag aacctcaatt
     1501 cctacaaaaa gaccctcctg ttatcaggtc ttaccggaat tgcatatctt ctgggtctgt
     1561 ttgttatgcc gactatggtg ctgtttgcca tgatcgtcgg gatatttacc attatacaat
     1621 ttgttattga tgtaatcagg ggtaaaacaa gcgaatacct cctcgtgata aacacggtaa
     1681 ttttttctat agcgattatc ggtcttctgg catacgggct gaaagatccc ggggttaacc
     1741 ttatctttta ttcaataggg catgtatatg cctacctggc attgatcggt ggaacgggtt
     1801 ttctctatgg tgttgcacgc tttgttaaag aaaaagactg gtattattac cctgcagcac
     1861 ttatcggaag cggaattgta ttttctctca tcctctttat tgcaagcccc cagttgtata
     1921 acctgtttat cagttcactg ttctcgtttt tcgggcagca ggcagttacc aataccgtgc
     1981 aggaggcccg cgggtgggca ccggatcttg cgtggtttac cttcaattat ggcattattc
     2041 ttatggcagg cggggcgctg gtcatgctct ataataacct caaagatgag cacccgcatc
     2101 agatctttgc acttgtatgg tctctcataa tgcttatttc aacatggcag catatccgtt
     2161 acgagtatta ccttgctgta aatgttgccc tcctctcagc cgtatgtgtc agctttacca
     2221 tagattatgc gtggacagac atccgccggc tcgcgacaag gatttcatcc gataaagatg
     2281 ttccagaatt caaaaaggag gtaaaagaag agacatccaa aggaaaaaaa cagaaaaaaa
     2341 ctatcaaaaa aagttcagac acctcaatgt cgggtttact tgcccttgtt tttgtcgttc
     2401 ttgccgtcgg gctggggatt ctttttgcct acacctccgc atcaatcagc tattcaaacg
     2461 ctgcgggaaa cacaatacgg atgaatcctg actggcggga aaccctggac tggatgggaa
     2521 ataatactcc tgataccggc gtgaactaca caactattta cgaccctaaa acattccagt
     2581 atcccccaca ggcgtatggt gtcatgtcct ggtgggatta tgggcacatg atcacctata
     2641 ttgcaaaacg gatcccgaat gcaaacccat tccaggctgg agttaccggc cctggaggat
     2701 cggcaaactt ctttatgtca atatccgaag atgaggggaa ccaggtcctg gaccatgcaa
     2761 aaacccggta tattatcacc gatatcgaaa tggataccgg taaattctgg gctatgtcaa
     2821 catggtataa tacatcagag ggtgcagccc cttaccagat gacgctcctt gcccctgcac
     2881 agaataatct taacagttat gagtcagttc tgctcaatac ccgcctctat tacttcacca
     2941 tgatctcaag actccataac tttgatggtt ctctaaaaac cccaacgagt gcctattatg
     3001 tggaatatgc agatccgagc atttcacagg tctctcttcc ggtcattacc gcagcagaag
     3061 cagttaatgc cactgatgca gtcagtcggg ctgctgatta caacctcagg gcatccgcag
     3121 gttaccatgc ctcagcattg agcccggcta tcacgttacc gattgaaaca gtaccagcac
     3181 tcagacatta ccggcttgtg catgaatccc ccagcaatgt gtttaattcg aataccactg
     3241 atgtgaaata tgtcaaagtt ttcgaatatg tgaaaggggc tcacatacaa ggtgagggga
     3301 ttattgaagt cccggttatc acaaataccg gaagaacctt cacctatcag caggcaagtg
     3361 ttaacgggga atttatcgta ccttattcga caacggggaa tccctatggg gtaaagacta
     3421 cggctaaata ccgggtcgtt tcttcaggac gaacatatga ggtacccgaa tcagctatca
     3481 tggacggggc acttgtccga taattttgat tcttttttaa aggtcataaa gaccaaacct
     3541 tttgttggca gcaacataat agagggtgac gaaaagtatg gtccggcgtt ctgtgattta
     3601 tggtgagatt ttcactcatc atgatatgga gaatcatgat gagagcgctg cccggctgga
     3661 agcagctcta gagggagtgc cagaaggcgt gccggttatt gcacctgaat gtgcccccga
     3721 gagtgatctg gcaagagtcc atgagcattc ttacatccac atgattcgcg agttttcttc
     3781 acacggagga caacattttc ttgacccgag cacctatgtt gatgcagaaa catataccgt
     3841 cgcctccttt gcagcaggcg ccgcaacaga agcagtaaac cgctctatag acggtgaagc
     3901 ctgttttgct atcatccgcc cgcccggaca tcacgccgaa cctgaccgtg ctatgggttt
     3961 ttgtattttc aataatgctg caatcgccgc ggccgttgcc cttgatcgtg ttgataaagt
     4021 cgcgataatc gactgggatc ttcaccacgg aaatggcacg cagaggatct tctccacaaa
     4081 cgatcatgta ctcttctgct cgatacacca gggaaatgtc tttccctata ccggctgggt
     4141 agatgaaata ggcacaggca gaggtaaggg ttttacaata aacgcacccc tgagagctgg
     4201 atcgaccatt gctgattacc ggcttgtctt tgaagaagtg ttcattcctg ctctcgaaca
     4261 attcagaccg gatgctgtaa tcatatctgc aggtcaggat gccctgtttg acgatcccaa
     4321 aggcggcatg atgcttttcc ctgcagatta tggcaccctt accggcattt taagagatgc
     4381 caccgataat cccctggcac ttgttctcga ggggggatac ggaccttcgc atggtgaagc
     4441 agtccggcag atattcaatt cacttcatgg atctcctgtt ccaccttgtc agtctgaacc
     4501 gcatcgcact acacgcgata tcatcgcagc cctcaaaaag ataaggatgt aatgaaaaat
     4561 gatttttgta acaacacctt tatagcgcaa tggagatcat tttcccatat gtctgcagaa
     4621 gagatcaccc ttgggatgcg ggtgaggtac ccgcgtacag gaaccactgg cacaattctg
     4681 cgcatcgagc aggttcgggg agaagcattc gccgaactgg ataccacaaa tctcctgtac
     4741 cgcatcgatt atcttatccc ggcgaccggg ccggagaaat caaccaagac cgttattgat
     4801 gacgccaaaa aagtaattga aagagaaaag gagtttgttt caggacgcga actccaggat
     4861 gcatggaaag ctactgacca gagctgcgaa ggaggagggt aattccccct taagtgattt
     4921 aaccgggcaa atccctgtta actcaataac tgttttatct tttaccggac ctcatcggat
     4981 ttttcagttt ccatcgataa atgcggtcac cagttcgggg aattcaattc agatataagg
     5041 aattgttatt cggataaaag tgatgatggt tcaaggcccc gtagggtctg ccgtttcagc
     5101 gagatactta tataatattt tcgcgtccat aagtgttatc aaaccagcag caactttctg
     5161 atcgatgtct cgttggatat ccgggtgcac aaatcaagaa agattaattc aggataaggt
     5221 gttggtgttc taaatttcac agagaagtaa ggaggttcga attttttgca tgatgtaaga
     5281 gttcccagtg ttaacatcgg ggtagttggc catgtcgatc acggcaaaac cactctggta
     5341 aacgcgctca cagggacatg gacagaccgg cacagtgaag aggtga
//
LOCUS       DASRLV010000150        10851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_54472,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000150 DASRLV010000000
VERSION     DASRLV010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 10851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10851
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54472"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VFC43_05945"
     CDS             complement(<1..240)
                     /locus_tag="VFC43_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30760.1"
                     /translation="MMSVVFGKEMRYCLIIFCLVLLMTPAAAGADAPAVTGITPSSGF
                     NTTTVSITDLSGANFTSGAMVILTPDTANPVHKGSI"
     gene            771..1193
                     /locus_tag="VFC43_05950"
     CDS             771..1193
                     /locus_tag="VFC43_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2111 domain-containing protein"
                     /protein_id="HZK30761.1"
                     /translation="MHRYSLSCSAEAGDLEPVVLAVHELIHRLPVTAKSVEKDGIRVE
                     DGRVVDRHYVGPVLLEAIERNIPIKTTPKSGAYKGVPVTVCPIRDHEGNAIGAIGVVD
                     ITGIFDLATLMEHQSAILKQVCGKDPCPLPSEQSGSRR"
     gene            1199..2179
                     /locus_tag="VFC43_05955"
     CDS             1199..2179
                     /locus_tag="VFC43_05955"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HZK30762.1"
                     /translation="MPDSAFKVLEILERTDKPLSGEALSNELGITRSAVWKHIQELRA
                     MGYDIFSSQKEGYKLTRTSNKLLPYEIHKKLKTQFIGKKIRYLENTPSTIWVGKQICA
                     EGDVGKMHGMVIIAEEQTGGIGRMGRAWVSPSGGIWITVVLKPRVPIDHIFMITMAGS
                     IAIARAIRKEFDLGALIKWPNDIFIGNKKVAGLLLELSADADSVHYCLLSIGIDVNVP
                     INQFSPALQKDITSISAEVGHEVDRAAFLARILKEFESRYLLIESGEYETIIREWKSL
                     SCTLENRVLIRTLKNSFEGDAIDIDEFGALIIRKDNGKLERVISGDCYQQ"
     gene            2230..2739
                     /locus_tag="VFC43_05960"
     CDS             2230..2739
                     /locus_tag="VFC43_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HZK30763.1"
                     /translation="MFGDLQKSRTIAYSAAFIGLISIGAWVSVPFFPVPLTLQTLFVL
                     LAGAVMKRNAIIPVFLYMVLGALGLPVFHTGATGFGILLGPTGGYLAGFVFAALVVGL
                     FYEHESATMKITGLLAGTGIIYLCGVSWLMYSLSVGFVPAVISGALPFLPGDLIKGYA
                     VYKIAGRLP"
     gene            2736..3401
                     /locus_tag="VFC43_05965"
     CDS             2736..3401
                     /locus_tag="VFC43_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HZK30764.1"
                     /translation="MITIDFLRYRNLAIDTLTIQPGNTSVIGPNGSGKTTFLRICAGI
                     SLPDTGTVMIDGQLPRETEIGWVNEFPDRNILFVNGFDEIASSLRFRNLPCASIDSSV
                     RSCAASMGITHLLKRPVREMSGGEKVLVSLAAALVCRPKVLIFDEYDSHLDARSISLI
                     DHILGKWGADYVIRCTQQMDTALRSDHLLFFDSGRVLHSGNPQQVIPLLEGTPWFPVF
                     GGC"
     gene            3404..4102
                     /locus_tag="VFC43_05970"
     CDS             3404..4102
                     /locus_tag="VFC43_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HZK30765.1"
                     /translation="MMISFDDVHAVRESWSLSVTGTFHEGVHLVSGDVGSGKTTLALM
                     MAGFFLPQEGLIDKKEIKSSILSHQFPEHSVTGISVADECISWSADTDEILRISEMKG
                     RESVSPLSLSRGELKRLHLACVLAKDCDLLLLDEPFSSLDCQEKVKLCDRISQKKKGI
                     CVIFTHEQNILPRVDRIWEITDGRLIECGMPPDAFFTWQHVPAVVTRLITSGQTPDNL
                     SLDDLREARCRIQG"
     gene            4084..4737
                     /locus_tag="VFC43_05975"
     CDS             4084..4737
                     /locus_tag="VFC43_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30766.1"
                     /translation="MQDPRIRIAAAAILSVAAFISLQGAVAAFLWWIVFTPRVTLVKR
                     IPGILSIILVIAFFSFFLEILGGGGLSYFIRMTVILLIGMWLYDEQKSGEFLAVGVWL
                     FGTRTGFEMGMTAEMGIQSLVSVAADLERIRMAETIKGVRWGVHSLVPAGLILVHGAL
                     IRAEDSAELLAVRGYRYGGSMCPKFITRSGDCIAGAIVLGVLIFSLIPVSEFFILYR"
     gene            4785..6521
                     /gene="oadA"
                     /locus_tag="VFC43_05980"
     CDS             4785..6521
                     /gene="oadA"
                     /locus_tag="VFC43_05980"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HZK30767.1"
                     /translation="MHTSQKKVFITDTTLRDAHQSLIATRLRTEDMLPLAREIDTCGF
                     FSVEAWGGATFDTCIRFLNDDPWDRLKALKSELKHTPIQMLLRGQNLVGYRHYPDDVV
                     DKFVTAAHKNGVDIFRVFDALNDIRNMKRSMDQVKNVGAHLQGAISYTTSPVHTTQTF
                     IDMAKDLYANDCDSICIKDMAGLIMPQATHDLIGGIKKAVDIKVCLHSHSTSGIAPMS
                     YQAAIESGVDILDTAMSPFAMGTSQPPTESVVASLIGTPAETGIDLHKLRAARNICIQ
                     IREKYAELLNPISERVDSDVLLYQLPGGMISNLVSQLQEQDALNRMDEVLAEIPRVRK
                     DLGYPPLVTPTSQIVGTQAVLNVLVNGHRYRNVTKEVKDYVHGLYGKTPAPVSGEIQK
                     LIIGDDEVVIVRPADLLEPAYEIMKAEAEKAGLVKKEEDILTYILYPAIAPSFLKGER
                     ILEEIPKKQPAVKPETGMASQMEVEVDGEVFTVRIVSVGGSKVEVTSVTQQKIPRGEF
                     AGGIKSNMQGMVLQVLVSRGASVKKGDTLLVLEAMKMENPIHSPVDGKVTEIFVDTGD
                     VVQNGDVLLVVQ"
     gene            6518..7993
                     /locus_tag="VFC43_05985"
     CDS             6518..7993
                     /locus_tag="VFC43_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HZK30768.1"
                     /translation="MKYFEKLLIANRGEIAIRVMRACRELGIETVAIYSSADNNALHV
                     KYADEAFLVGDAHPSKSYLNIERIVDIAKKSGAEAIHPGYGFLAENYRFAKICQDENL
                     IFVGPRWKTIKAMGSKIGSKQMMKGAGVPVVPGTEGGVKSFDEAKKVAADIGYPVIIK
                     ASAGGGGIGMQIVNDEQGLQEAIAAAMRIAESAFGDATILIEKYLVKPRHIELQVLAD
                     EHGNAVHLYDRECSIQRRHQKLIEEAPSPIMSTELREKMSASALKVAEVSGYSNAGTV
                     EFLYSNGSYYFMEMNTRLQVEHTITEFITGIDIVRQQIAIAAGESLPFEQRDLSIRGH
                     AIECRINAEDPLNNFSADPGKITRYRSPGGPGIRIDSGIHMGYRIPPNYDSMIAKLCA
                     WDSTRSEAITRMRRAISEYIILGIKTTLPLHYAIMNNQQFMAGNTHTHFLQEEHILST
                     LERYKRDEETRMQTLAGSFGEGKKVAAISAAVNIYMQAKKG"
     gene            8061..8172
                     /locus_tag="VFC43_05990"
     tRNA            join(8061..8098,8137..8172)
                     /locus_tag="VFC43_05990"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8095..8097,aa:Met,seq:cat)
     gene            8343..8843
                     /locus_tag="VFC43_05995"
     CDS             8343..8843
                     /locus_tag="VFC43_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HZK30769.1"
                     /translation="MLFANAVVGLIQLLISIIFAVLALYIGFAVLSKINKNMDEEKEL
                     AKGNAAVGIIVAAVFVAIALVVQSGVSGLSVGINKALSVGFFSVDGLLAIGISFVQLI
                     LGIVLAVGAIYLALNILDKLTKGVEEFEEIKKGNVAVALEMAGVIIAVAVIIQSGVIG
                     ITAALI"
     gene            complement(8893..10119)
                     /gene="mmp10"
                     /locus_tag="VFC43_06000"
     CDS             complement(8893..10119)
                     /gene="mmp10"
                     /locus_tag="VFC43_06000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867903.1"
                     /note="Mmp10 (methanogenesis marker protein 10) is a
                     cobalamin-requiring radical SAM methyltransferase that
                     creates the methylarginine modification to methyl coenzyme
                     M reductase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl coenzyme M reductase-arginine
                     methyltransferase Mmp10"
                     /protein_id="HZK30770.1"
                     /translation="MAQLTVDIGGSPGINCRGFCEYCYFKKVRGVQPLGCRYCLPFKK
                     GCDYCTRGVKEEYSGFKDLKTIADETLANLQQKRGDLERITISGGGDPSCYPRFEELI
                     ELLGSMEAPLHIGYTSGKGWDDSGIADMLIENGLSEISFTVFATDPALRKRYMHDPTP
                     EASLAILEKLCGSVDVYAAAVILPGINDGSVLEQTCTWLQDRGAKGLILMRFANATEQ
                     GLILGNGPIIKNQEVQTVESFRDLISDLNKRFTMKISGTPLWDPDIGSPFAILNEPDL
                     LEKLPLVQRRATVISGSIAAPFIEKVLVSRGSHVPVIPVKKEIACLITIDDLHHLDLR
                     ALEPTVIIPGRAFVQDAEAEAVLCRDGISRIILRGPEMLTADAETSMGMTQEQVLSME
                     MDGFADLINVINQNGR"
     gene            10237..10309
                     /locus_tag="VFC43_06005"
     tRNA            10237..10309
                     /locus_tag="VFC43_06005"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10270..10272,aa:Thr,seq:tgt)
     gene            10461..>10851
                     /locus_tag="VFC43_06010"
     CDS             10461..>10851
                     /locus_tag="VFC43_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30771.1"
                     /translation="MNNDDAFSPTFTTILMVMTALTTIMFGGLIGIYAYEITGSIHNE
                     RLIVISVVQSGPDILVKYEGGDARPPALYSMTVIGPNGLPYFTVSSSGRLSTSGTPDA
                     PAAGSVMVLSGAATQNQDHVLVIAYFAD"
BASE COUNT         2763 a         2524 c         2707 g         2857 t
ORIGIN      
        1 aatgctgcct ttatgaaccg ggttggcagt atccggtgtc agtatgacca tcgcaccgga
       61 agtgaaatta gcaccgctca gatccgtgat cgataccgtg gttgtgttga aaccggaaga
      121 aggggtaata cccgtcactg caggtgcatc cgcaccggct gctgccgggg tcattagcag
      181 tacaagacaa aaaatgatca gacaataacg catttctttt ccaaaaacta ctgacatcat
      241 tccacccatc ctgatcccct tttttagaat tcctgatatt ttgttatgcc gggaattatt
      301 tccgtaaata tttgttctca tttgtgtcct tcgctttttt aatttataga cattagcgac
      361 aatactgacg ctcacgcagt gttcctgaaa tgttcatcct cagggtatat atgtagatta
      421 ttttataatc ctatataggt atggaaaaga acaaaaagtg aaatgagact tgttcaaatg
      481 aacattcggg atcagcagga gggttcatga ttgctcttgt tccagattct gcggctgtga
      541 tatattatgt gattgaataa ataaccaaaa tttccggatt caacacggat ttatcgtcaa
      601 actcatacca aaaaatgttt cataaaaacc tttatcagaa acgttgaatt atcctccacc
      661 gtatatccaa tgggaaattt ttcatattct taatagacaa ttacttctca cgatgcgcaa
      721 tcttcttcta gatagaaatt gaccagtgtt ataagagagg tattcgtcat atgcaccgtt
      781 attcgttgtc ctgcagtgcg gaagcaggcg atcttgagcc agttgtcctc gcagtccacg
      841 aattgatcca ccgccttccc gttacagcca agtcagtgga aaaagatggc atacgtgttg
      901 aagacgggag agttgttgac cgccattatg taggtcctgt tctccttgaa gcaatagaga
      961 gaaatattcc tataaaaact accccgaaaa gcggggctta caagggagtt cccgtgacag
     1021 tctgtcccat cagggaccat gagggaaacg caatcggggc aataggggta gtagatatca
     1081 cggggatttt tgatcttgcc accctgatgg aacaccagtc tgcgatactc aagcaggttt
     1141 gcggcaaaga tccctgtcct cttccttcag aacagagcgg ttcaaggagg tgaatatttt
     1201 gccggattct gccttcaaag tgcttgagat tctcgaacga actgataaac ctctatccgg
     1261 agaagctctg agcaatgaac tgggaattac ccggtctgcg gtctggaagc atatccagga
     1321 gctgcgggcg atgggctatg acatcttctc ctcgcaaaaa gagggttata agctcacccg
     1381 tacgagcaac aagctccttc cttatgagat acataaaaag ctaaaaacac agttcattgg
     1441 aaaaaagatc cggtacctcg aaaacactcc ctcgaccatc tgggttggca agcagatatg
     1501 cgcagaaggg gatgtcggga agatgcacgg tatggtgatc attgccgaag aacagaccgg
     1561 ggggataggc agaatgggaa gggcctgggt ctcacccagt ggcgggatct ggattacggt
     1621 agttctcaaa ccccgcgtac ctattgatca tatcttcatg atcacgatgg cgggatccat
     1681 tgcgattgca cgggcaatcc gcaaggaatt tgatctcggt gccctcataa aatggccaaa
     1741 tgatatattt attggtaaca agaaagtcgc aggactcctt cttgagctct cagcagatgc
     1801 agactcggtc cactactgcc tgttaagcat aggtattgat gtaaatgtac cgataaacca
     1861 gttttcgcct gcgttacaaa aagacatcac ttctatcagt gctgaagtcg ggcatgaagt
     1921 ggaccgtgct gcctttctcg cacgcattct caaagagttc gagagcaggt acctgctgat
     1981 tgagagcggg gaatatgaaa caatcatcag ggaatggaaa agtctttcct gtactcttga
     2041 aaaccgggtg ctgatcagga ccctgaagaa cagtttcgaa ggggatgcaa tcgatataga
     2101 tgagttcggc gctctcatta tcaggaagga taatggaaag ctcgagcggg ttatctccgg
     2161 cgactgttat cagcaataat taacaggtaa ttttttatcg tcaaccattc aattttttta
     2221 tggttgacga tgtttggaga cctgcagaaa tccaggacta ttgcatactc tgccgcattt
     2281 attggtctca tcagcatagg cgcatgggtc tctgtcccct tctttcctgt accgctgaca
     2341 ctccagacgc tttttgtcct gctcgccgga gcagtcatga agaggaatgc aataattccg
     2401 gtcttcctgt acatggttct gggtgcactc ggtctccccg tgtttcatac cggagccaca
     2461 gggtttggta tcctgctcgg ccctaccgga ggttacctgg cagggtttgt ttttgcagcc
     2521 cttgttgtcg gactgttcta tgagcacgag tctgcgacca tgaagataac cgggctcctc
     2581 gctgggaccg gtattattta cctctgtggt gtttcctggc tgatgtattc gctttctgtc
     2641 ggtttcgtcc ctgccgtgat cagtggcgct cttccgttcc tccccggtga tttgataaaa
     2701 gggtatgccg tgtataaaat tgccgggcga cttccatgat caccattgac ttcctccgtt
     2761 atcgaaatct tgcgattgat acccttacca tccagcccgg gaatacctcc gttattggcc
     2821 ctaacggcag tgggaaaact acatttctca ggatctgtgc agggatttcg ctgcctgaca
     2881 ccggtacggt gatgatcgat ggccagcttc cccgggaaac tgaaattggc tgggtgaacg
     2941 agtttcctga ccggaatatt ctttttgtca atggttttga tgagatcgcc tcttcacttc
     3001 gtttccgaaa ccttccctgt gcgagtattg attcgtcagt caggtcctgt gctgcatcta
     3061 tgggtattac tcatctcctc aaaagaccgg tccgtgaaat gtccggtgga gaaaaagtgc
     3121 tcgtttccct tgctgctgca ctcgtctgcc gcccgaaggt tctcattttc gatgagtatg
     3181 attcacatct cgatgcccgg tcaatttctc tgatcgacca catactgggt aaatggggtg
     3241 ctgattacgt tatccggtgc acgcagcaga tggacaccgc tctcaggagt gatcatctcc
     3301 tttttttcga ttcgggaagg gtactacatt cggggaaccc ccaacaggtc attcctttac
     3361 tggaaggaac accctggttc ccggttttcg gggggtgctg acaatgatga tctcatttga
     3421 tgatgtacac gctgttcgtg aatcctggtc cctgtctgta accggcacat tccacgaagg
     3481 agtgcacctt gtcagtggag atgtggggag cgggaagacg acacttgccc tcatgatggc
     3541 aggattcttc ttacctcagg agggactcat cgataaaaaa gagattaaat ccagcatact
     3601 ttcacaccag ttccccgaac actccgtgac cgggatttct gtcgctgatg agtgtatatc
     3661 ctggtccgca gataccgatg aaattcttag gatttccgaa atgaaaggga gggaatctgt
     3721 ttcgccgctc tcgctgagca ggggtgaact gaagcggctt caccttgcgt gcgtgcttgc
     3781 aaaggactgt gacctcctgt tgcttgacga acccttcagc tcgcttgact gccaggaaaa
     3841 agtaaaatta tgcgatcgga tttcccaaaa gaaaaagggc atttgtgtta tttttaccca
     3901 tgaacagaat attcttcccc gggttgaccg gatttgggag atcacagacg gccgattgat
     3961 cgaatgcggt atgccgccgg atgcgttttt tacatggcaa catgtacctg cggttgtaac
     4021 cagactcatt acatccgggc agacgccgga taacctgtcg ctggatgatc tccgggaggc
     4081 ccgatgcagg atccaaggat aagaatagct gctgctgcaa tcctctcggt tgcagcgttc
     4141 atcagtcttc agggagcggt tgccgcattc ctgtggtgga ttgttttcac cccccgcgtt
     4201 acgctggtaa aaaggatccc cgggatttta tcaataatcc ttgtgatagc atttttcagt
     4261 tttttcctgg agatactggg tggggggggc ctgtcgtatt ttatccgcat gaccgtgatc
     4321 ctgcttattg gtatgtggtt atatgatgag cagaagtccg gggaatttct ggctgttggc
     4381 gtctggttgt tcggcaccag gacaggattt gagatgggga tgactgccga gatgggaata
     4441 caatccctgg tatcagtggc cgccgatctc gaaaggatcc ggatggcaga gaccatcaag
     4501 ggtgtgcgat ggggagtgca cagccttgtt ccggccgggc tcattttagt tcacggggct
     4561 cttatcaggg cagaagacag cgcagaactc cttgcagtcc ggggatatcg ttacgggggg
     4621 agcatgtgtc caaaatttat tactagatcg ggtgattgta ttgccggcgc gattgttttg
     4681 ggagttttga ttttttccct gattccagtt agtgaatttt ttatacttta ccggtgaagg
     4741 tttgagaggc ttaatatgat cctacggaag ccctgaggat cctcatgcac acctctcaaa
     4801 aaaaagtttt catcaccgat accaccctgc gggatgccca ccagtcactc atagcgacca
     4861 ggctccggac cgaagatatg cttccgctcg cacgggagat agacacctgc ggtttttttt
     4921 ctgttgaagc ctggggaggt gcaacattcg atacatgcat acggtttttg aatgacgatc
     4981 cctgggaccg tctcaaagcc ctgaaatctg aacttaagca cactccaatc cagatgctcc
     5041 tgcgcgggca aaatctcgta ggctaccgcc attatcccga tgatgtggtc gataaattcg
     5101 taactgcagc acacaaaaac ggggttgata tcttccgggt ttttgatgca ttaaacgata
     5161 tcaggaacat gaagcggtca atggaccagg taaaaaacgt gggtgctcac ctgcagggag
     5221 caatatccta tacaacgagt ccggtgcaca ccacccagac atttattgac atggcaaaag
     5281 atctctatgc gaatgactgc gactctatct gtatcaagga tatggccggg cttatcatgc
     5341 cgcaagcgac ccacgatctg attggcggca taaaaaaagc agtggatatc aaggtctgcc
     5401 tgcacagcca ttccaccagc ggaattgcac cgatgagtta ccaggctgct atcgagtccg
     5461 gcgtggatat tcttgataca gcaatgtccc cgtttgcgat gggaacttcc cagccaccga
     5521 ccgagagtgt tgtcgcatct cttattggca caccagcgga aacgggtatt gacctccata
     5581 aactcagggc ggcccgtaac atctgcatac agatcaggga aaagtacgcc gaattgctca
     5641 acccgatttc cgagcgtgtt gacagtgatg tactccttta ccagttgccg ggcggcatga
     5701 tctccaacct tgtttcgcag ctgcaggaac aggatgctct caaccggatg gatgaagtgc
     5761 ttgcagaaat tccaagggta agaaaagatc ttggctatcc ccctcttgtt acacctacaa
     5821 gccagattgt cgggacccag gctgtgctca acgtcctggt caatggtcat cgataccgga
     5881 atgtgactaa agaagtcaaa gactacgttc acgggctcta cgggaaaact cctgcacccg
     5941 tgagcggaga gattcaaaaa ctcatcatcg gggatgatga agtggttatt gtccgcccgg
     6001 cggatctctt agaacccgca tatgagataa tgaaggcaga agccgagaaa gcaggactgg
     6061 ttaaaaaaga agaagatatt cttacctata tcctgtatcc tgccatcgcg ccgtctttcc
     6121 tcaaagggga acggatattg gaagagatac ccaaaaagca accggcagta aaacctgaaa
     6181 ccgggatggc cagccagatg gaagtggaag tggatggtga agtcttcacc gtcaggattg
     6241 tttcggtagg gggaagtaaa gttgaagtta ccagtgtcac ccagcagaag atcccgcggg
     6301 gtgaattcgc cggtggcatc aagagtaata tgcagggtat ggtgctccag gtgctggtta
     6361 gccgtggtgc atcggtgaag aagggggata ctcttttagt ccttgaggca atgaagatgg
     6421 aaaatcccat ccatagcccg gttgacggca aggtcaccga gatctttgtg gataccggcg
     6481 atgtcgtcca gaacggagac gtcctgctgg tggtgcaatg aagtattttg aaaaactcct
     6541 gattgcaaac aggggggaga ttgctatccg cgtaatgcgg gcatgcaggg aacttggtat
     6601 tgaaaccgta gcgatctact cttctgcgga caataatgca ctccatgtca aatatgcaga
     6661 tgaggcattt cttgtaggcg atgcacatcc ttcaaaaagc tacctcaaca tagagagaat
     6721 tgtcgatatt gcaaaaaaaa gcggtgctga ggcaattcat cccggttacg gttttttagc
     6781 ggagaattac cggtttgcga aaatctgcca ggatgagaac ctgatctttg tcggcccccg
     6841 ctggaagacc atcaaggcga tgggatcgaa gatcggcagc aaacagatga tgaaaggggc
     6901 aggagttccc gtagttcctg gtaccgaggg tggcgtaaaa agtttcgatg aagcaaaaaa
     6961 agttgctgcc gatatcggat acccggtaat tatcaaagcc agcgcaggag gcggaggtat
     7021 cgggatgcag atcgtgaacg atgagcaggg tttgcaggag gcaattgctg ctgccatgcg
     7081 tattgcagag tctgcattcg gtgacgccac gattttaatc gagaaatacc tggtaaagcc
     7141 ccggcatatc gagctgcagg tgctggctga tgagcacggt aatgcggtcc acctgtatga
     7201 ccgtgaatgt tccatccagc gccgtcacca gaaactcatt gaagaggctc cgtctccgat
     7261 catgagcact gaattgcgtg aaaagatgtc agcatccgca ctcaaggttg ctgaagtatc
     7321 aggatattcg aatgcgggga cggttgaatt cctgtacagt aatgggagtt attattttat
     7381 ggaaatgaac acccggctcc aggtagagca taccattacg gagtttatca ccggaatcga
     7441 tatcgtaagg cagcagatcg caattgctgc cggtgagtcc ctgccttttg aacagcgcga
     7501 cctctcaatt cgcggtcatg caattgagtg ccggatcaat gcagaagatc cgctgaataa
     7561 cttctcagcc gacccgggaa aaattacccg ttaccgatct ccgggaggac ccggtatcag
     7621 aatcgatagt ggtattcaca tggggtacag gattccaccc aattatgatt caatgatagc
     7681 aaaactgtgc gcatgggata gcacgaggtc tgaagcaata acaaggatga gacgtgcaat
     7741 ttctgagtac atcattcttg gaatcaaaac gaccctcccc ctccattacg cgatcatgaa
     7801 caaccagcag tttatggccg gtaatacgca cacgcacttt ttgcaggaag aacatatcct
     7861 gagtaccctc gaacggtaca aacgtgacga ggagactcgg atgcagacac ttgccggttc
     7921 ctttggagag ggaaagaaag ttgctgcaat atcggcagcg gtgaacatat atatgcaggc
     7981 aaaaaagggc tgatgcccaa aaaaaacccc gcatcagaac ctttaatagt ttttacggcg
     8041 tccttattat gcacttttgt gctgcggtgg cctagctggt tagggcgcca gactcatagg
     8101 gtttaggaat ggaatacgcg ttcatggctt tgagagatct ggagatcggg ggatcgtaac
     8161 cctcccgcag cattggatac cctgccgaag aatcccgttt ttactccttg tgttgctgtt
     8221 tctctgtcga ttgaggtttt tcacaattcc tgtcatttaa gagattgcat gattgcatat
     8281 catctgacga tatgtttatt tgtggcctta tcaaacaaaa ttacgtaaat tgaggtgtaa
     8341 atatgctttt tgcaaacgca gttgtaggat taatccagtt acttatctcg attatctttg
     8401 ctgtcctggc attgtatatc ggatttgctg tactcagcaa gataaataaa aacatggatg
     8461 aggaaaaaga gctcgccaaa gggaatgcag ctgtcgggat tattgttgca gcggtatttg
     8521 tcgcaattgc tctcgtcgtt cagtccggag tgtccggctt atccgttggc atcaataagg
     8581 cgctcagcgt aggatttttt tctgttgacg ggctgcttgc tatcggtata tcgtttgtcc
     8641 agctcatcct cgggattgtt cttgccgtcg gagccatcta tcttgccctg aacattcttg
     8701 acaaacttac aaaaggtgtc gaagagttcg aagagattaa aaaaggtaat gttgcagtcg
     8761 ccctcgagat ggcaggggta attattgcgg ttgcagtaat catccagtcc ggtgtaatcg
     8821 gaataactgc agcactgata taaattcaca ttattttttt aatgtttcaa cccaaaaaaa
     8881 tagcaatcct tcttatcgcc cattctggtt tatcacgttg attaaatcgg caaatccgtc
     8941 catttccatt gaaaggacct gttcctgcgt catccccatg cttgtttcgg catcggccgt
     9001 cagcatttca ggacctctca ggataatgcg gctaatacca tccctgcaga gcactgcctc
     9061 agcttcagca tcctgcacaa aggcccgccc ggggataatc accgttggtt caagagcccg
     9121 aagatccagg tggtgaaggt catcgatcgt tattaagcag gcaatctctt ttttcacagg
     9181 aatcacgggc acgtgggagc cgcgtgaaac cagcactttt tcaataaaag gtgctgcaat
     9241 gctgccgctg atgaccgttg ccctacgctg cacgagggga agtttctcca ggaggtccgg
     9301 ctcattcagg attgcaaaag gtgaaccgat atccgggtcc cacagcgggg tcccggagat
     9361 tttcatcgtg aaccttttgt tcaaatctga tatgaggtcc cggaaggact caacggtctg
     9421 gacttcctgg ttcttgatta tgggaccatt cccaaggata agcccctgct ctgttgcatt
     9481 cgcaaaccgc atcaggatta ggccttttgc cccccggtcc tggagccatg tacaggtctg
     9541 ttcgagcacc gaaccgtcat tgattcccgg caggatgacc gcagcagcat aaacatcaac
     9601 gctaccgcac aatttttcga ggattgccag agaagcttca ggagttggat cgtgcatgta
     9661 gcgcttacga agagcggggt cagttgcaaa gaccgtaaaa gagatttcag aaagtccgtt
     9721 ttcaataagc atatccgcta tacccgaatc atcccagccc ttaccactgg tgtacccgat
     9781 atggagaggg gcttccatgc tgccgagaag ttcgatcaat tcttcaaacc ggggataaca
     9841 actcgggtcc cctccaccac tgatcgtgat ccgttcaaga tctcctctct tttgctgcag
     9901 gtttgcgagt gtctcgtctg ctatggtctt gagatccttg aatccactgt actcttcttt
     9961 aacaccacgg gtacagtaat cacacccttt tttgaatggc aggcaatacc tgcatcccag
    10021 tggctgaaca ccccgcactt tcttaaaata gcagtattcg caaaatccac ggcagttgat
    10081 accggggctt cctccaatat ccacggtcag ctgtgccatc agtataaatt actcagttct
    10141 tgtgcgtaaa aggtgtttgg gattgaacac aaaagaacag cggaaagtga atttcatcat
    10201 catctttttt actatcaaat gtctaaatac tatggcgcct cagtggctta gttggcagag
    10261 cggctgactt gtaatcagca ggtcccgtgt tcaaatcacg gctgaggctt gaaaaaggaa
    10321 cttcgatttt accttatttt cttaaaataa acgtaatttt aaggtattta atgtttttga
    10381 gatatgattg cccagacagg taccttaaat cgtttaataa agaaactatc tcttacaaag
    10441 atatttcttc cctgttatct atgaacaacg atgatgcatt ttcgccgaca ttcacgacaa
    10501 ttctcatggt catgactgca ctaaccacca ttatgtttgg aggactcatc ggcatatatg
    10561 cttacgagat tacagggagc atccataatg aacggttgat tgtaataagt gttgtgcaat
    10621 cgggtcctga catcctggtc aagtacgaag gtggagacgc ccgcccgcct gccctttatt
    10681 ctatgaccgt tattggtccc aatggtctgc catatttcac agtaagttcc agcgggagat
    10741 tatcaacgag cggtaccccg gatgcgccag ctgccggctc tgtcatggta ttatcaggtg
    10801 ctgctaccca gaaccaagat catgttttgg ttatcgctta ttttgcggac g
//
LOCUS       DASRLV010000151         3996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_941573,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000151 DASRLV010000000
VERSION     DASRLV010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941573"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFC43_06015"
     CDS             <1..258
                     /locus_tag="VFC43_06015"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZK30772.1"
                     /translation="TVYGRIYRDVRQVPYGSTSTYGQVAERVGTSPRVVGQAMARNPT
                     PLIIPCHRITAAHGIGGFSPSIEIKEALLAMEKKELRKVKR"
     gene            354..944
                     /locus_tag="VFC43_06020"
     CDS             354..944
                     /locus_tag="VFC43_06020"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HZK30773.1"
                     /translation="MRSAVVLVGGEARRVNGQEKYFFTYLGRTFIERLVDSLQEVVDE
                     IILVARNPEQCKRFRKIEGIRCITDIREGIGPIGGLHAGSVAAQGDLIFVSACDMPCI
                     NPEVVSYLFDCIDNYDAVIPIWNPDMLEPLHAVYRKTALLAYLESHDSLSLRSMIMNL
                     SAKYVPVDELKNIDPELLTFTNINKIEDLERINTSG"
     gene            1104..1955
                     /locus_tag="VFC43_06025"
     CDS             1104..1955
                     /locus_tag="VFC43_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZK30774.1"
                     /translation="MEQLSVENKEVLRLFLVTATCIGAFLTSIFSLMNGIFSVFPFHY
                     ILPIILVVYLYPERGVLFSLGLSIMYISLIYLLGYSDPIQIAIGTAWFAIFITIGVVA
                     SSYAIRQREERTRVRNILDNSQDGIFCFNLKDLHIRDINPKCAQWLKYNQEDLIGKDI
                     TVIWTDKEEQRQFIDEMNRETKRTHTPREAHFLRQDGDVARFVISPMLVTQGQVLCSA
                     IDITRSKIVDEEIIKALDDLELQVRERTADLERINEKLRAEILECRRFESTVLSGHPL
                     QPKREDI"
     gene            1952..3397
                     /locus_tag="VFC43_06030"
     CDS             1952..3397
                     /locus_tag="VFC43_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK30775.1"
                     /translation="MKPPVTIRPRFFWMAMIVISIIIAIYATVFSLTHGIYEVFPFLY
                     FLPIILFVNFYPNRGVVFSLTISTIFLALVYFFSNFDTTLIAVSTAWFVIFVTIGFVT
                     SSFAEGSKAEERKYREIFENSQAGIFTFALTTLHIQEINGKCASMLGYERHDLIDKDL
                     SRILPDSADRDLFISQILKNSGVGDTELLLHTRDGLVRQFLVSASLSPGNRVICSAID
                     ITERKLAERVIQKARGDLERKVKERSEELMRANDGLKAEILERKRFEATLKLANRKLN
                     TLSSITHHDILDQINAIVSYVSVAEAIVTDPSLLEHLKKIDQRTQSIQKQIRFARDYE
                     DIGLNAPQWQAVDDTIRRAITELDLAGVRIEKEVDRLEIFTDLLLEKVFYNIVDNALR
                     HGQKETKIWFSARETGGGLTIFCQDDGVGVPASAKEGIFKCDYFRNKGYGLFLAAEIL
                     SMTGLSIRETGEPGSGARIEITVPKGLYRFI"
     gene            complement(3684..>3996)
                     /locus_tag="VFC43_06035"
     CDS             complement(3684..>3996)
                     /locus_tag="VFC43_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HZK30776.1"
                     /translation="VNGYVTALMDDLNLGAYDRIAICGPEVMMRAVLTKVEEKGIAHK
                     TEFSLHRYMKCGVGVCGSCCIDPSGLRVCRDGPVFSGDLLLKSEFGHYMRDASGRKKN
                     V"
BASE COUNT         1071 a          922 c          936 g         1067 t
ORIGIN      
        1 acggtatatg gcaggatata ccgggatgtt cgacaggtac cgtatgggtc cacttcaaca
       61 tacgggcagg tcgcagaacg ggtcgggaca tctcctcgcg tagtcggtca ggcaatggca
      121 cgcaatccca cgccccttat cataccctgt caccgtatca ccgcggctca tggaatcggg
      181 gggttctctc cctccattga gatcaaggag gcattgcttg ccatggaaaa gaaagagttg
      241 cgcaaagtga aaaggtgaca ctctgcagcc gcacaaccct tgtcgggtcc atgagatgtt
      301 cattttttaa agaatatcag caaaggtgat aatggtatga acggagaacc gcagtgagat
      361 ctgcagtcgt actggttggt ggagaagccc ggcgtgtaaa cggtcaggaa aaatattttt
      421 ttacgtacct tggcaggaca ttcatcgaac gtctggtcga ttcgctccag gaagtggtcg
      481 atgagattat ccttgtcgcc agaaatcctg aacagtgcaa aaggttccgg aagattgagg
      541 gaatacgatg catcactgat atccgcgagg ggattggacc gattggaggt cttcatgccg
      601 ggtcggttgc tgcacaggga gatctgatct ttgtctccgc gtgtgatatg ccctgtatca
      661 atccagaggt tgtttcatac cttttcgact gcattgacaa ttacgatgcg gtgataccca
      721 tctggaatcc cgatatgctc gaacctcttc atgcggtcta ccggaagaca gccctccttg
      781 cttatctgga aagtcatgat tcgctatcac tgcgttcaat gatcatgaac ctgtctgcaa
      841 agtatgtccc ggttgatgag ttaaaaaata tagatcctga actcctgaca ttcaccaaca
      901 tcaacaaaat cgaggatctt gagcgtatca acacatcagg ttaggatata tagacttttt
      961 ctcaaaaaac gaaaaaaata taagcgatct ggcgattgtg tgggaactta ccacagagta
     1021 tataaatata tggaattaga tcattaataa tagtatacca gtccttactt cggtttggct
     1081 gggaattcgg caggggacag ccagtggagc aattatctgt tgaaaataag gaagtattac
     1141 gactgttcct ggttactgcc acctgtatcg gagctttcct cacgtcgatt ttttccctca
     1201 tgaacggtat ttttagtgtc tttccctttc actatatcct gcctatcatc ctcgttgtct
     1261 atctctaccc ggaacgggga gtactctttt cactgggatt aagcatcatg tatatcagcc
     1321 tcatttacct tctggggtat tctgacccta tacagatcgc aatcgggaca gcatggtttg
     1381 cgatctttat cacgattggt gttgtggcct catcctatgc aatcaggcaa cgtgaagaac
     1441 gtacccgcgt caggaatatc cttgacaatt cacaggacgg catcttttgt tttaatctta
     1501 aggacctgca cattcgtgat attaatccca aatgcgccca gtggctgaag tacaatcagg
     1561 aggatctcat cggtaaggat atcaccgtca tctggaccga caaagaagag cagaggcaat
     1621 tcatagatga aatgaaccgg gaaacaaagc gaacacatac tccaagggag gcacacttcc
     1681 tcaggcagga tggggatgta gccaggtttg tcatttcccc tatgcttgtt acccagggac
     1741 aagtattatg ttctgcaata gatatcacca gaagtaaaat cgtagatgaa gaaattatta
     1801 aagctctcga tgatcttgaa ctacaggtaa gagagcgtac agcggacctg gaacggataa
     1861 acgaaaaact ccgggcagag atccttgaat gccggcggtt cgaaagcacg gtcctgtccg
     1921 gccatcccct gcaaccaaaa agggaggaca tatgaaacct cctgttacca tccggccccg
     1981 ttttttctgg atggcgatga tcgtcatttc cattatcatt gcaatctacg caacggtttt
     2041 ttccctgact cacggtatat atgaggtctt tccgttcctc tacttcctgc cgatcatcct
     2101 ctttgttaat ttttatccaa accggggtgt ggtcttttcg ctgaccataa gtacgatctt
     2161 tcttgctctg gtctattttt tcagcaattt tgacacgacc cttatcgcgg tatcaacagc
     2221 atggtttgtt atcttcgtaa caataggatt tgttacgtcc tcatttgccg aagggtcgaa
     2281 agcagaagag agaaaatacc gggaaatatt cgagaattct caggctggta tattcacctt
     2341 tgcactcacg accctgcata ttcaggaaat aaacgggaaa tgcgcctcta tgctgggata
     2401 tgagaggcac gacctgatcg ataaggatct cagccgcatc ctcccggatt ctgctgacag
     2461 agatctgttc atcagccaga tactgaagaa ttcaggagtt ggggatactg aacttctcct
     2521 tcacacacgg gatggcttag tacgccagtt ccttgtttct gcatcacttt ctcccggcaa
     2581 cagggtaatc tgttctgcca tcgatattac cgaacgtaaa cttgcagaac gggttattca
     2641 gaaagcacgg ggagacctgg agagaaaagt aaaagagcgg tccgaagagc tcatgcgggc
     2701 aaatgacggg ctcaaagcag agatccttga acgaaaacga tttgaagcaa cactcaagct
     2761 cgcaaaccgc aaacttaaca cactctcttc cattacccat cacgatattc tcgaccagat
     2821 caatgcgatt gtcagttatg tctctgttgc agaagcgata gtcaccgatc cttccctcct
     2881 tgagcatctt aaaaagatcg atcagagaac gcagtcgatc cagaaacaga tccggtttgc
     2941 ccgcgattac gaggatattg ggctaaacgc accccagtgg caggcagttg atgataccat
     3001 cagacgagca atcacggaac ttgatcttgc gggtgtccgg atagaaaaag aagtggacag
     3061 gcttgagata tttacggatt tgttactgga gaaggtattc tataatattg tggataacgc
     3121 actgcgtcac gggcaaaagg agacaaaaat atggttctct gcccgggaaa ccggtggggg
     3181 gttgacaatt ttctgccagg atgacggtgt aggtgttccc gcaagtgcaa aagagggaat
     3241 tttcaaatgc gattatttcc ggaacaaagg ctatggtctt ttcctggctg ccgaaattct
     3301 gagtatgaca ggactctcta tccgcgaaac cggagaaccg ggttccggag cacgtattga
     3361 aatcacggtt cccaaaggtt tataccggtt tatttaaaaa aggtgattag gactttgttg
     3421 aaatcctctt tttctcagta tttaattatt cccggcggca tgtaggaaga tgacacccct
     3481 tttttttatt ggggtttttc atcaacctca atggagttat cgcattaatg ggggatgcct
     3541 gagcggcggg gacaaagccc ccgggaacgg gcatacattt ttagttttca tcgggatctg
     3601 agttgggtcg atgttaccca ataacctgat agtataattg gtgaatgacg ggaatttttc
     3661 atccatgctg ctattttttc ttatcagaca tttttttttc tgccactcgc atcccgcata
     3721 taatgaccaa actcactttt gagaagaaga tcccctgaaa agaccgggcc atccctgcaa
     3781 acccggagtc ctgatgggtc tatgcaacat gaaccacaga cacccacccc gcatttcatg
     3841 tagcggtgca gcgaaaattc agttttatgg gcgattcctt tttcttcgac tttggtaagc
     3901 accgccctca tcatcacttc cggaccgcag attgcgatcc tgtcatatgc accaaggttc
     3961 aggtcatcca taagcgcagt tacatacccg ttaacg
//
LOCUS       DASRLV010000152         6188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1110220,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000152 DASRLV010000000
VERSION     DASRLV010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6188
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110220"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VFC43_06040"
     CDS             <1..490
                     /locus_tag="VFC43_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HZK30777.1"
                     /translation="AVQAVARMRESDNDLLKPFGKAIRLVIGLFCTETFDYQALILGK
                     LKTQYRLDPNDIEKLNVKGKLEVLKQDGSTTVVPLAELETCIRKGCHFCTDLTAVLSD
                     ISAGAIGSPAGSTTLIIRSPTGNGFVSSAVRNHRLDISKGVDIAAIEKLAVAKIKKNT
                     RK"
     gene            complement(1201..2565)
                     /gene="cca"
                     /locus_tag="VFC43_06045"
     CDS             complement(1201..2565)
                     /gene="cca"
                     /locus_tag="VFC43_06045"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HZK30778.1"
                     /translation="MVKRLPVEEKVLLELRPTMQERDHVCGLAKRLLDTIALSGKAEG
                     MVVGSIARNTWVRGDRDLDVFMLFDPGLSREALESEGLGLARSIAAAFTDKFHEKYAE
                     HPYINACIDDVDVDLVPCYKVDSATSIQSAVDRTPFHTRYITDRIHGLIDDVLLLKQF
                     TKAGGIYGSDQMTEGFSGYLCELLVLHYGGFTPLLKAAASWRPGMVIDIENHAAKKFD
                     EPLVVIDPVDPKRNVAAAVSLDRMAGFVELARGYAENPLTEFFLHPMERCILKKELSA
                     RIEQRGTYVYAVTFATPRLIPEIVVPQLKRSVLSIRELLERNGFPVHHASYDMQSEEC
                     MILLELLVGNLPAIRRHPGPPLWNNENADKFRKKHLTEHKSGPFIEDGRYVMEVPREY
                     RTARSLLSSGPLLSVSLGKHIRQSLELGWTLHEGAECWKEEFAPFIAEFFDQSSPLAR
                     IQRT"
     gene            complement(2559..3098)
                     /gene="thpR"
                     /locus_tag="VFC43_06050"
     CDS             complement(2559..3098)
                     /gene="thpR"
                     /locus_tag="VFC43_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HZK30779.1"
                     /translation="MVRAFVALELSQEIKDNLSAAQNTVRTCNARLTFVNPENMHITL
                     KFLGDVQQEKLKKVMDAIRSVTFSPFPIQARTVTVNNPKRPHTVWCDIEDGGMGERLF
                     ILIENALAPLGFNKENRRFNPHATLARVKSMDPSLFLAINNLKEKSWGNCIISGITLK
                     KSTLLPRGPVYEDLFEVKW"
     gene            3229..3498
                     /locus_tag="VFC43_06055"
     CDS             3229..3498
                     /locus_tag="VFC43_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HZK30780.1"
                     /translation="MSKKSQLIVYTLDSCPNCENLKGFLTKGGYRFVERDLSTAESLT
                     ELRMNGVFVNEAPVLQYDSDFYTSADMFPSGKLDGEHLSTLISGE"
     gene            3498..5681
                     /gene="nrdD"
                     /locus_tag="VFC43_06060"
     CDS             3498..5681
                     /gene="nrdD"
                     /locus_tag="VFC43_06060"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HZK30781.1"
                     /translation="MAHRETKQLTFEGITVPTLPFVRTSDGHIIEWDRSRIVQQLVQE
                     TKLVETFYGYEGADEATAQEIAFDVENRIKNMGLKSLSGPLIREIVNITLLEKGMIQY
                     RNVSTRVGTPVFDAHQIDVGRGFEAHDNANLQENAETSHKKKADKISKEQYLLQLPPD
                     LADHHLSGEMHIHDLEYFGTRPFCQDWDLRYFFYYGLMPDGNGTKASVAGPAKRAEVA
                     ILHAVKALGSAQTNFAGGQGYYNFLTFLAPYMEGMVYDEIKQNMQMFVYEMTQMMVAR
                     GGQLVFSSIQLSPGVPTLWQDKPCVYKGKIWDGTSSPRRTYGEFEREVRLLFKALMEV
                     MLEGDYWGKPFNFPKPEISIEPDFMTEREEFNHKNPDLPTFNELYLMTFELASKFGTP
                     YYDNQLPAYRGAGKGISCYQCCAYQFSTVADKDSDFEKKLIFENGKHFSMGSWQVVSV
                     NCPRAAYDAEGDDARLFAELKSLMDVAVEIFKIKRRWMDLIRANSRMPFAMQRPKDPN
                     TGERGALAVDLEGLVYTVGIVGVNEMVQHHTGKQLHESKAAFKLAIRAMTEMELYGRE
                     LSKKNNMTIALARTPAETTGQRFAVADLLDRRYHNFAKNVIKGDINEGLQMLGKSRDL
                     PVYYTNGTHVAPAANVTLTKRMEIEHVFFPIVDGGNIFHIWLGEARPDPRGLMDMAMK
                     LCRTTQIGYFAFTRDITVSLRQFHEMRQEKDAITSWEHADLPVKA"
     gene            5978..>6188
                     /locus_tag="VFC43_06065"
     CDS             5978..>6188
                     /locus_tag="VFC43_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30782.1"
                     /translation="MPASLSLWKKPMLKLCSTEKLPWYGNSVRYPEENFSFLKREKPY
                     SVWYFFEGKIHNPVMNAVIAVDSVNR"
BASE COUNT         1606 a         1427 c         1529 g         1626 t
ORIGIN      
        1 tgccgttcag gcagtagcac ggatgcggga aagtgataat gaccttttaa agccgtttgg
       61 gaaagcgatc cgtctcgtga tcggtctttt ctgcacagaa acgtttgatt atcaggcgct
      121 tattctggga aaactaaaaa cgcagtacag actcgatccc aacgatattg aaaaacttaa
      181 tgtgaaggga aaactggagg tgctgaagca ggatggcagt acaacggtcg tgccgctggc
      241 agaactggaa acctgcatcc gtaaaggctg ccatttctgc actgacctta ctgcggtcct
      301 ttcggatatc tctgccggtg cgatcgggag tccggccgga tccacaacac tgattatccg
      361 ctccccgacg gggaatggat tcgtatccag tgctgtcagg aaccacagac ttgatatcag
      421 taagggagtt gacattgcgg ccattgaaaa actggcagtg gcaaaaatta agaaaaatac
      481 ccggaaataa ctttttttac aaataataca tggtggactg tgtgtttttt tcacccgccc
      541 aggtaacccc ccgggtgtta tgtatacatt tttgggtgca ttgtaaacat agatgtcaag
      601 tctaaggggc gggcttcacc cgccggtcca gcagtcatgt ttgactgaaa aaggcttgcg
      661 cggaacgaag aacgggttct aaaacgcttc tatgggctcg ctacggggta ttgtagaggg
      721 agaatgtatc cccccttttc gctcaccatc ccccgagggg gagagggtgc aaggcaggat
      781 agccctcttg agtgggggac gtgctccaga ccgtgaccat gaacatcgcc ctgtgatcca
      841 cgtgtagcga agcgtcactc caatcttttt tacgattttt ggctctgtag aaatcctttg
      901 aataatactg acgctcttgg gggcatgcaa gcatgccccc gccgctgtgg catcccccga
      961 ttgcgattat gctgtattac ctttacgcaa ttgtcgaaaa atttccagtt tttgtaatgc
     1021 cgaggcatcg taatgcgcct cctcccccaa tgggggcatg gttggcgcaa ggggggcgat
     1081 cataacttga aaagaggatt tctatagagc acaattttca cagtggactg ttttttttcc
     1141 acaaaaatag cataaaagtc tgattgaaga cgttatccag aaaaaaatat atgattctgt
     1201 ttatgtccgt tgaattcttg ccaggggtga gctctggtca aagaattcag cgataaatgg
     1261 cgcaaattcc tccttccagc actccgctcc ttcatggaga gtccaaccca gttccagcga
     1321 ttgccggata tgtttcccaa gactgacact tagcagggga cctgatgaca gcagacttct
     1381 tgcagttctg tattcccttg gcacctccat aacgtaccgt ccgtcttcaa taaatggtcc
     1441 tgacttatgt tctgtgaggt gtttttttcg gaatttatct gcattttcgt tattccagag
     1501 cggtggcccc gggtgtcgcc ggattgcagg aaggttaccc accagcagct caaggaggat
     1561 catgcactct tctgactgca tgtcatacga agcatgatgg acagggaacc cgtttctttc
     1621 gagaagttcc cggatcgaca aaacgctgcg cttcagctga ggcacaacaa tctccggaat
     1681 cagccgggga gttgcaaagg tgactgcata cacatatgta ccccgttgtt cgattcgtgc
     1741 agataattct tttttcagaa tgcagcgttc catgggatgt aaaaaaaatt cggtaagagg
     1801 gttttcagca tatcctctcg ccagttcaac aaatcctgcc atacgatcga gagatacagc
     1861 tgctgcaaca ttgcgtttcg ggtcaacggg atcgatcaca actaatggct catcaaattt
     1921 ttttgccgca tggttctcga tatcaattac catcccgggc cgccatgaag cagcagcttt
     1981 caaaagggga gtaaatcccc cgtagtgcaa cacgagaagt tcgcagagat agcctgaaaa
     2041 accttcggtc atctggtctg aaccatagat gccgccggcc tttgtgaatt gcttaagcag
     2101 gagcacatca tcaatcaacc cgtgtatcct gtcagtgata taacgggtat gaaatggtgt
     2161 gcgatccacg gcactctgga tgcttgtggc actatcaact ttatagcacg ggacaagatc
     2221 cacatccaca tcgtcaatgc aggcgttgat ataaggatgt tcggcgtatt tttcgtggaa
     2281 tttatccgta aaagcagcag caatactacg ggcgaggcca agtccttcag attcgagtgc
     2341 ttctctcgat aagcccgggt caaagagcat gaatacatcg aggtcacggt ctccacgaac
     2401 ccaggtgttc cgagcaatgg aacctacgac cattccctcg gccttcccgc tcaatgcgat
     2461 tgtatcaagg agacgctttg ccagaccaca gacatgatcg cgttcctgca ttgtcggacg
     2521 gagttccaga agcacctttt cttcaaccgg gagccgtttc accattttac ctcaaacaga
     2581 tcttcgtaga ccgggcccct gggcaggagt gtactcttct ttaacgttat gcctgagatt
     2641 atgcagttgc cccaggattt ttccttcagg ttgttgattg ccaaaaaaag ggatgggtcc
     2701 atagatttga cccgtgccag ggtggcatgg gggttgaagc gtctgttctc cttattaaat
     2761 ccaagcggtg caagagcatt ctctatcagg atgaataaac gctcccccat tcccccgtcc
     2821 tcgatatcgc accagactgt atgcggtctt ttcggattat tgactgtaac ggtccgtgcc
     2881 tgaatcggaa agggggaaaa tgtgactgat ctgatggcat ccatcacttt tttaagtttt
     2941 tcctgttgca cgtcaccaag aaattttagc gtgatgtgca tattttcagg atttacaaaa
     3001 gtaaggcgtg cattgcaggt gcggaccgta ttctgggctg cggagaggtt gtcctttatt
     3061 tcctgcgata gttcgagtgc aacaaacgcc cgtaccatta cggtactatt atttcattcc
     3121 agtactaatg gtaatcgctt tttatgggta caccggatgc aagttcaaac catgattttt
     3181 ttatctccag aaaacctatt ggataaatga ctttgtaagg gtgttcagat gtcgaaaaaa
     3241 tcgcaattga ttgtttatac gcttgattct tgcccgaatt gtgagaattt gaagggattt
     3301 ctgacaaagg gcggctatag gtttgttgag cgtgacctct caactgcgga atcactcact
     3361 gaactgcgga tgaatggagt ttttgtgaac gaagcccctg ttttacagta tgattctgat
     3421 ttttacacct ctgcggatat gtttccgtca ggcaaactgg atggagagca cctatcaacg
     3481 ctgatatcag gtgaataatg gcacaccggg agaccaaaca acttactttt gagggaatca
     3541 ctgttcccac actccccttt gtccgcacat ctgatgggca tattattgaa tgggatcgct
     3601 cccgaatcgt ccagcagttg gttcaggaaa cgaaacttgt tgaaacattc tatggatatg
     3661 agggtgctga cgaagcaacg gcccaggaaa tagcgtttga tgtggaaaac cgcatcaaaa
     3721 atatggggct gaagtccctc tccggtccgc tcatccgtga aatagtgaat attaccctgc
     3781 ttgaaaaagg gatgattcag taccgtaatg tatccacacg ggtcggtaca ccggtttttg
     3841 acgcgcacca gatcgatgtt ggccggggat ttgaggcgca tgataatgca aacctccagg
     3901 aaaacgccga gacctcgcac aagaagaagg cggataagat atccaaggaa caatacctgc
     3961 ttcagctccc gcctgacctc gcggatcacc acctctccgg agagatgcat atccatgatc
     4021 ttgagtattt cggcacccgt cctttttgtc aggactggga tcttcgttat ttcttctact
     4081 acggtttaat gcctgatgga aacggcacta aagcatccgt tgcaggcccc gcaaaacgtg
     4141 ctgaagttgc aatccttcat gcagtgaaag cactgggttc tgcccagaca aattttgccg
     4201 gcgggcaggg ttactataat ttccttacat tcctggcgcc ctacatggaa gggatggtgt
     4261 acgatgaaat caaacagaac atgcagatgt tcgtgtatga aatgacccag atgatggttg
     4321 caaggggtgg tcagctcgtc ttctcctcaa tccagttatc cccaggagtc ccgactctgt
     4381 ggcaggacaa accgtgtgta tacaagggaa agatctggga tggtacatca tctcctcgca
     4441 ggacctacgg ggaatttgaa cgcgaagtac gtctgttatt caaggcactc atggaagtga
     4501 tgctcgaggg agactattgg ggaaagcctt tcaattttcc aaagccggag atttccatcg
     4561 agccggactt catgactgaa cgggaagagt tcaatcataa aaatcctgat ttgcctacat
     4621 ttaacgagtt atatctgatg acattcgaac ttgcttcgaa attcgggacc ccctattatg
     4681 ataaccagct gcccgcttac cggggtgcag gcaaggggat tagctgttac cagtgctgtg
     4741 cctaccagtt ctctaccgtg gcagacaagg attctgattt cgaaaagaaa ctgatctttg
     4801 agaatgggaa gcacttttcg atgggttcat ggcaggttgt atcggtgaat tgcccccggg
     4861 ctgcttatga tgcggaaggt gatgatgccc ggctctttgc agaactcaag tcccttatgg
     4921 atgtcgcggt tgaaatcttc aaaattaagc gccgctggat ggacctgatc cgggcaaaca
     4981 gccggatgcc atttgccatg cagcgcccga aagatcccaa taccggtgaa cggggtgcac
     5041 ttgcagttga tcttgaagga ctggtatata ctgttggtat tgtcggagtc aacgagatgg
     5101 tacagcacca tacaggaaaa cagctccacg aatcaaaagc cgcttttaag ctggctatac
     5161 gggcaatgac tgaaatggag ctctatggtc gtgaattatc aaagaagaac aatatgacca
     5221 tagctctcgc acgaacaccg gcagaaacta caggacagag gtttgcggtg gcagacctcc
     5281 ttgacaggcg ttaccacaat tttgcaaaaa atgtaatcaa gggggatatc aatgaaggtc
     5341 ttcagatgct gggaaagagc cgggacctgc cggtgtacta tacaaacggt acccatgtgg
     5401 caccggcggc gaatgtaacg cttacgaaaa ggatggagat cgagcatgta tttttcccga
     5461 ttgtggatgg tgggaacata ttccatatct ggttaggcga agcacgtcct gatccccgcg
     5521 ggcttatgga tatggcgatg aaactgtgcc ggacaaccca gatcggatat tttgccttta
     5581 ccagggatat taccgtctcg cttcggcagt tccatgaaat gcggcaggaa aaagatgcaa
     5641 tcacttcctg ggagcatgcc gatctcccgg taaaagcctg atcgtatagt ttttttgctt
     5701 ttaacctatt attttatgat ttcctgtcag ttcccgacca tacggccacc tgccccgggg
     5761 aatatccagg tgtattttac gaaaagaaat tttctttgga tcctacgcat taccttcccg
     5821 ataaatcatc cgaccgcaag gccacagcga ttgaagttaa ggaagtccgg tatttcccga
     5881 aatacttcgg gaccgaatat ggggaagatt ttggatattc gcatggatca catcttgcct
     5941 acttctatcc ggttaggatg gttaccttgt atatgaagtg ccagcatctc tttctctgtg
     6001 gaaaaagcca atgttgaaat tgtgttcaac ggaaaaacta ccgtggtatg gaaactcggt
     6061 cagatatcca gaagaaaatt tttctttttt aaaaagagaa aaaccttatt cagtgtggta
     6121 cttttttgag ggtaaaatcc ataaccccgt gatgaatgcg gtaatagctg tggattctgt
     6181 caataggg
//
LOCUS       DASRLV010000153         5539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_544830,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000153 DASRLV010000000
VERSION     DASRLV010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5539
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544830"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VFC43_06070"
     CDS             <1..996
                     /locus_tag="VFC43_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZK30783.1"
                     /translation="KLGLLIFTIFIVAIIAFWPLMTVFIWAIAVAVALLPIQKKLRGI
                     VKPSVSATFITVTVLLLILLIMSLAASLLVNNQKHIADMALSMITGLQHTRFSAFLPS
                     FSEAQLNNFDQTLNELMVKAILSLTGNIMQILLSIIIFFLSLSMLLYYGDTIWNTATG
                     AFSEKLLNAVCRLSEITENTIYSLIVVQISAAVIAFALAIPFFYFLGYGDILLFSSLI
                     GFAMLIPLIGAQLMILLLALYFLSLGDIRGVLAMLFIGYPLLSGWIDFYYRPVMMGKK
                     VAVHPVVMMIGIFAGVPFMGIVGFILGPVLIALVVTGYKILADEEFAQKGCTRTA"
     gene            1099..1521
                     /locus_tag="VFC43_06075"
     CDS             1099..1521
                     /locus_tag="VFC43_06075"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZK30784.1"
                     /translation="MVTMPEELIHLLTEWHTVPVATTGPGGKPNVAAKSVMVMNPETL
                     VWGELYFMQTWENLKKDPRASICVWKRSPPFNAYKINGRVTMHENDVIATQLDERMQT
                     GHGMDFKPRREKLAAVYFTVEEIYDQTPDIKSAGRQIT"
     gene            complement(1653..2618)
                     /locus_tag="VFC43_06080"
     CDS             complement(1653..2618)
                     /locus_tag="VFC43_06080"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HZK30785.1"
                     /translation="MTLKSDISMKQSGLHKQGSVSRRFILIPLFLCMLFSVGASAIPT
                     ANFTGTPLTGIAPLSVTFTDLSTGAGLTNWSWNFGDGNITNLTASANPVHIYLTAGLK
                     SVNLTVTDGDGSNSTIRTGYINVLEPEPEPESHGFNTGLFRNSSGTWYLDTTKTGAVN
                     RTLQFGKSGDTAVTGDWNNDGTTNVGVFRPASGTWYLETAKTGVVNKTFQFGKSGDIA
                     VVGDWNNDGLTNAGVFRPASGTWYLDTTHTGTIFRTFQFGKSGDIPVVGDWDNDGLSN
                     AGVFRPASGTWYLDTTHTGVVNKTFHFGTTGDSPVVGKWIENIIL"
     gene            complement(3191..3451)
                     /locus_tag="VFC43_06085"
     CDS             complement(3191..3451)
                     /locus_tag="VFC43_06085"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HZK30786.1"
                     /translation="MKEEKTGLTQELACIDGAYRLNQTGRRIPEMASFTCRDIGIDCP
                     FEAHAITDQELMRKFIDHAESDYKMQVLSADVILRIQDAIKR"
     gene            complement(3600..3872)
                     /locus_tag="VFC43_06090"
     CDS             complement(3600..3872)
                     /locus_tag="VFC43_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30787.1"
                     /translation="MIYYISDPHQSKSSIERVSIYILSGVARSVAKPEINRFSGVLFR
                     KAMHHEPVRTRFFVPCLPVMNRTIAISDPDAGDTKSADFYDLLHNL"
     gene            complement(4089..4436)
                     /locus_tag="VFC43_06095"
     CDS             complement(4089..4436)
                     /locus_tag="VFC43_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30788.1"
                     /translation="MLQKQWRNLKSTGISGVLTGWAIHTEPVRANSRIPDFDRAATIV
                     KNTSREDRCFEFPSLSAVFFPGVSACSKSAGPGKELWYSHKQDTGEDSHHRQPEYIKN
                     AGNKICIEKSRGC"
     gene            4554..4784
                     /locus_tag="VFC43_06100"
     CDS             4554..4784
                     /locus_tag="VFC43_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30789.1"
                     /translation="MTDNLKVKSGKAVNDTRVAAHAVYDDATVAAHSTLKNAGAEAQK
                     LSDDVKIGVHKAVSDAKIAVHGAGAALKKAEI"
     gene            complement(5143..>5539)
                     /locus_tag="VFC43_06105"
     CDS             complement(5143..>5539)
                     /locus_tag="VFC43_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HZK30790.1"
                     /translation="GIPRIIVSTGKETTLSFAQHTFPAGSVSVVPQSTVDDVVKKSLE
                     PVVLSLLEKPISGYDIVREIHSRYKVLIPQARVYTILHDLMEEGYLEIRVSGKSKLYC
                     TTEKGKIHVSQKLKDFRQVFQHVWGTKVE"
BASE COUNT         1427 a         1349 c         1273 g         1490 t
ORIGIN      
        1 aaactggggc tcctcatttt caccatattc atcgtggcga tcatcgcatt ctggccgctg
       61 atgaccgtct ttatctgggc gattgcggtt gcggttgcac ttttaccaat acaaaaaaag
      121 ctccggggga tcgtaaaacc ctcggtatcg gccacattca tcacggttac ggtgctgtta
      181 ctcatcctgc tgatcatgtc gcttgctgca tccttactgg tcaacaacca gaagcatatc
      241 gcggatatgg cactttccat gatcaccgga ttacaacaca cccggttttc ggcattcctg
      301 ccctcatttt ccgaggccca gctgaataat tttgaccaga cgctcaatga actgatggta
      361 aaggccatcc tgtccctgac cggaaatatc atgcagatcc tgctctcgat catcatattc
      421 ttcctctcgt tatccatgct gctctattat ggtgatacga tctggaacac cgctacggga
      481 gccttttcag aaaaactctt aaatgccgtg tgcaggttat cggagattac cgaaaatacc
      541 atatattcct taattgtcgt ccagatctcg gcagcagtga tcgcatttgc ccttgcaatc
      601 ccgttctttt actttttagg gtacggggac atactcctgt tttcctccct gatcgggttt
      661 gcaatgctca ttcccctcat cggggcacaa ctcatgatcc ttctccttgc actctatttc
      721 ctgagccttg gcgacatccg tggcgtgctt gccatgctgt ttatcgggta tcccctgctg
      781 agcgggtgga ttgatttcta ttaccggccc gtcatgatgg ggaaaaaagt tgccgtccat
      841 ccggtggtca tgatgatcgg catctttgcc ggggtgccgt tcatgggcat tgtcgggttc
      901 atcctcggcc cggtgctcat cgcgctcgtg gtgaccggtt ataagatact ggcagacgag
      961 gagttcgccc aaaagggctg taccaggaca gcctgagagg catcctcgca tcacagggta
     1021 tcctcaccag ccgcaaggga aaaaccttcc caatgtttat cagggctgat ggcaatccac
     1081 tcatggatgt gaatcaccat ggttacgatg cctgaagaac tgatacacct gctcactgaa
     1141 tggcatacgg ttccggttgc gactaccgga ccggggggaa aacccaacgt tgcggctaaa
     1201 tcggtcatgg tcatgaaccc ggaaacactt gtgtggggtg aactgtattt tatgcagacc
     1261 tgggaaaatc tcaaaaaaga tccccgggca tccatctgtg tctggaaaag aagcccccct
     1321 ttcaatgcct acaagataaa cggaagggta accatgcatg aaaatgatgt gatcgcaact
     1381 cagctggacg agcgcatgca gaccggccat gggatggatt tcaaaccccg ccgggaaaag
     1441 ctggcagcag tctattttac tgttgaggaa atctacgacc agacacccga cattaagtct
     1501 gccggaagac agattacctg atattggtca gacgctaaac tgagtgtatt tcgcattttt
     1561 tttatttcca tgacagagaa atgatataat caacaaaaaa gagagctaaa aaacgaacgg
     1621 ttcgtcctca aaactcccgc ggcaaaaaaa aattacagga ttatattttc aatccatttc
     1681 cccactaccg gagaatctcc ggtcgtgccg aagtggaagg tcttgttgac cactcccgta
     1741 tgcgtggtgt cgagatacca ggtaccggac gccgggcgga atactcctgc attcgatagt
     1801 ccgtcgttat cccagtcccc aactaccggg atatctccgg atttcccgaa ctggaaggtc
     1861 cggaagattg ttcctgtgtg cgtggtgtca agataccagg tacccgaggc tggccggaat
     1921 actcctgcat ttgtcagtcc gtcgttattc cagtcaccga caaccgcaat atctccggat
     1981 ttcccgaact ggaacgtctt gttgacaacg cccgtcttcg cggtttcaag ataccaggtg
     2041 ccggaagccg gccggaatac tcccacattc gtggttccgt cattattcca gtcaccagtt
     2101 accgcagtat ctcctgattt tccgaactgg agagtcctgt tgactgcccc ggtcttcgtg
     2161 gtgtcaagat accatgtgcc ggacgaattc ctgaatagac cggtattgaa cccgtgtgat
     2221 tccggctccg gctccggctc cagtacattg atataaccgg tcctgattgt tgagtttgaa
     2281 ccatccccgt cagtaacggt caggttcacg cttttcagtc ccgccgtcag gtagatatgt
     2341 accggattag cactggctgt caggttcgtg atgttcccat caccgaaatt ccagctccag
     2401 ttggtgagcc cggcaccagt agagaggtca gtgaatgtaa ctgataaggg ggctataccg
     2461 gtgagtggtg tccctgtgaa gttcgccgta gggattgcac tggcgcccac agaaaacagc
     2521 atgcagagaa acaaggggat cagaatgaag cgccttgaga cgcttccttg tttatgcagt
     2581 cctgactgct tcatcgaaat atcagatttt agtgtcatga agatacgtcc ttttgtttaa
     2641 ttctcctaca agaaattatg gatgatacct ccgctacagc ttactcgagg ccataccgaa
     2701 gaatgtcgaa gtctattttt ttcttattat attaaccaat cctcatgtta caaaatttga
     2761 atcatccggg aacaaccggt tgcagtatat tttactgtta aggaaatcta cgaccagacg
     2821 ccggacctca aggcagccgg aaaacggatt acctgagacc acgtgaacaa ttctcagtca
     2881 tgtcccctaa tctcaatttt ttaggatctc ctcaccgggg gtctggccgg gagtagttaa
     2941 accggaaaaa gtgggtttta aggcaggagt ccccgggtct gttttccctg gcgacagcta
     3001 tcggagcaga ggggggaggg ggagaatctt tttttatatc atacactgct ctcgggagtt
     3061 ttacttaaat aacgagagaa tcgggatgat agggcagaat aacctgcctg ctcagacgaa
     3121 aaagaactgt ccttcgacgg ggaatgagta taaaaagagt tcatactttt aataacgaga
     3181 tctgaaaaaa ttatctttta atggcgtcct gtatccgtaa aatgacatct gcggacagga
     3241 cctgcatttt atagtcagat tccgcatggt cgataaattt tctcataagc tcctgatcag
     3301 ttattgcgtg cgcttcaaac gggcaatcga tcccgatgtc tctgcaggta aatgatgcca
     3361 tttccggaat tctcctaccg gtctgattca agcgatatgc accatcgata catgccagct
     3421 cctgtgtaag gccagtcttt tcttctttca ttgaatcctc atagttatcc ttgaaatttg
     3481 cgggaattgt ttcaaaatgc cttccgaatg cttcagattt atcaaagaac ccctcgcttc
     3541 agagatgcgt gaaatagccg ctaccgatat aaatcggtga tccatcattg caggtttcat
     3601 tacaaattat gcaaaagatc ataaaaatca gcagatttcg tatcccccgc gtcaggatca
     3661 gatatcgcta ttgttctgtt catcacgggg agacacggga caaagaaccg ggttcttaca
     3721 ggttcatggt gcatggcctt cctgaataaa acaccggaaa atctgttaat ttcaggtttc
     3781 gccactgatc ttgcaacacc ggacaggatg tatatactta ctctctctat tgaagactta
     3841 ctctgatgtg ggtctgaaat gtagtatatc atgatatttt ttacccgtca aatcagggaa
     3901 aggagttgaa tacaaaatgt ttgacagtct gaaagtaaaa tccggcaaag ccatagatga
     3961 cacccgcgtg gcggtacaaa accgtttttc cctgaggttc gatctgaaaa acaaccggac
     4021 atacatggaa catcttcatt acccataaca caatctgata tctgcaaatc ttctccatag
     4081 tgtgaagatc agcaacccct gctcttttca atgcaaattt tattacctgc attttttatg
     4141 tattccggct ggcgatgatg ggaatcttcg ccggtgtcct gtttatggga ataccagagt
     4201 tcttttcccg gcccggcgct cttcgaacag gcactaacac cgggaaaaaa taccgcagat
     4261 aaggatggaa attcaaagca ccggtcttca cgggaagtgt tttttactat cgtggcagcc
     4321 cggtcaaaat ccggaatacg ggaattggct cttacaggtt cagtgtgtat ggcccatcct
     4381 gttaaaacac ccgaaattcc tgttgatttc agatttcgcc actgtttttg caacaccggg
     4441 caggatgtat atacctgctt tctctatatg gacttactct gatatgggca aaaaatgtat
     4501 cttatcatga tattttttaa ccatcatatc gggggaaagg agttgaaaac accatgaccg
     4561 acaacctgaa agtaaaatcc ggcaaagccg tcaatgacac tcgcgtagcc gcacatgcag
     4621 tatatgatga tgcaactgtc gcggcacata gtacccttaa aaatgcaggc gcagaagcac
     4681 agaaactatc ggatgatgta aaaatcggtg tccacaaagc cgtttctgat gcaaaaatcg
     4741 cggttcacgg ggccggagca gcattgaaga aggcggaaat ctgattatcc cctcttattt
     4801 catacatttt taagtactcc catacttttt ctctcatccg aactaaaccg ggatctatta
     4861 ccgtgtaaaa ctgtttgaga ttctctcttc cctgagtata attctcgtgt gatcgttgtc
     4921 tgcatacaca ttgtgatggg gaatggattt ggcggatgct ataatcttca tgtaatccca
     4981 atcgggagac tctcaattcc ctacatggca gcaggggata tggaaaggtc atcccgctgc
     5041 caggaccacc gcaaagatgg agaaacagtt atatggaggg acgggtgcaa aggatcgttg
     5101 attctcccct tccaggacct tcgctcacga gaatccccgt tattattcca ctttcgttcc
     5161 ccagacatgc tggaacacct gcctgaagtc tttgagtttc tggctgacat gtatttttcc
     5221 tttttccgta gtacaataca atttggattt tcctgaaacc ctgatctcta ggtacccctc
     5281 ctccataaga tcgtggagga tggtgtaaac ccttgcctgc gggattagga ccttgtaccg
     5341 ggaatggatc tcgcgaacga tatcataccc tgaaattggc ttttccagaa gagaaaggac
     5401 caccggttca agtgactttt ttacgacatc atcgacggtt gactggggga ctacggaaac
     5461 agatccggca ggaaacgtat gctgggcgaa cgaaagcgtt gtttcttttc cggttgatac
     5521 aatgatcctg ggaataccc
//
LOCUS       DASRLV010000154         7079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1187282,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000154 DASRLV010000000
VERSION     DASRLV010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 7079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7079
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187282"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..125
                     /locus_tag="VFC43_06110"
     tRNA            54..125
                     /locus_tag="VFC43_06110"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:86..88,aa:Ala,seq:ggc)
     gene            258..887
                     /locus_tag="VFC43_06115"
     CDS             258..887
                     /locus_tag="VFC43_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HZK30791.1"
                     /translation="MRIRDLILPEDKVFFTLFKAMAEKIVEASTILNEITHELPGGTE
                     KAHRVRQIEHQGDEITRQIYEQLNESLITPLEPEEIARLAPVLDDVLDKIDWVTHQLC
                     NYGIPASNDVLKEYSYLIHLTGNEISNAITKLALLKNPEEVKTHAIEINRLYNLSTEL
                     LSRAVLELFKTNDLLMIIKLKDIYEGLAKVMETCNDVGHALNDIAMSHA"
     gene            884..2074
                     /locus_tag="VFC43_06120"
     CDS             884..2074
                     /locus_tag="VFC43_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HZK30792.1"
                     /translation="MIGMEPLILFGIILALALNFVNGLNDASHSIATVVATKALSPIK
                     AVFSTAICNMIGPFLFSTAVAATIGTAIVTRDALTPLSIVVAMGASIILVFVATRMGI
                     PISSSHAMVGGLLGAGIGAAGFSAVILPGAETVFEVIFYACIGAVIGALVLSIICAAF
                     EGDIRLGVLIGGVCGAGMIIPALMITGVIHIQGLLAIVLFILISPLIGMAGAFLFNVL
                     VSNLIKHARQNRAKRFFQPLHVLACLVQATAHGANDGQHAVGVITALLLSSGALLVFD
                     VPQWVILTSAIAIGLGTCFGGWQVVDKMAKEITKIRPYQGFCAATASSAILVLVTHQG
                     IPVSSTHAINGAIVGVGATRGKNAVQWEVVREMMSAWVITIPLALAVSWTGFLIISVV
                     LKLF"
     gene            2136..2765
                     /locus_tag="VFC43_06125"
     CDS             2136..2765
                     /locus_tag="VFC43_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HZK30793.1"
                     /translation="MGLRELLIPHDKIFFDMFEKQAAVVKEAAWQLVALTEDFTSVKE
                     KRHAIEKLEHKGDMITHDIYEQLNRTFITPLDPEEISRLASTLDEVLDYIDGSAEKMF
                     YYNIEGTDSHMVELAKLIHMSTAEIESAVKGIRSLKDPRYVEERCIEVNRLENLADDV
                     LAHAVTDLFKTNDAITIIKLKDIYEHLETATDYCEDVANVLSDIAIRHS"
     gene            2797..3828
                     /locus_tag="VFC43_06130"
     CDS             2797..3828
                     /locus_tag="VFC43_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HZK30794.1"
                     /translation="MIEATWEIIIFIIGIALIFDFTNGFHDSANSISTVVSTKVLSPR
                     NAVAFAAFFNFIAAFGFGVAVASTISKIIKLDFVQTGLIPYIILSALIGAIAWNLITW
                     FFGLPTSSSHALIGGLSGAGISAAGLAAIKWSTVELVATFMVISPIIGLACGFLFMAV
                     VLNLTKKANKQSAEIYFKRLQLLSAAAYSFSHGTNDAQKTMGIIIPLLFSIGYFGAGV
                     DPNALPVPLWVILIAHAAIALGTLSGGWRIVKTMGYKITKLRPVQGFAAETAGATTII
                     GASLIGIPVSTTHIICSSIMGVGTTMGASTVKWGVARSIVMAWILTIPISALIGFVAF
                     AVIRIIIGY"
     gene            complement(4404..5054)
                     /gene="nth"
                     /locus_tag="VFC43_06135"
     CDS             complement(4404..5054)
                     /gene="nth"
                     /locus_tag="VFC43_06135"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HZK30795.1"
                     /translation="MKKQDAATIYSLLVQRYPDARNSPAMLSKGTAFEVLILTILSAQ
                     TTDRAVIKVRDPLFSKYPRPSDLAAADIGDVEKIIHSLGYFHAKAKNIILASRALLSD
                     FGGDVPETMEELLKIPGVGRKTANIVLYHALGKNEGIAVDTHVRRLAQRIGFSDTDNV
                     TVIEKDLMAAFPQELWGDLTDVLISHGRECCDAKKPRCEICPISRYCRFFKTSFKK"
     gene            complement(5130..5204)
                     /locus_tag="VFC43_06140"
     tRNA            complement(5130..5204)
                     /locus_tag="VFC43_06140"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5166..5168),aa:Pro,seq:tgg)
     gene            complement(5271..5975)
                     /gene="pyrH"
                     /locus_tag="VFC43_06145"
     CDS             complement(5271..5975)
                     /gene="pyrH"
                     /locus_tag="VFC43_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HZK30796.1"
                     /translation="MKTIVMSLGGSILIPLLEKNKIKEYIPVLSEIAAQHRLFVVVGG
                     GGEARRYITVARDLGIDEGTSDELGILITRLNANLLIAALGERAYPKVAESHGEAKKF
                     AESGKIVVMGGITPGQTTDAVAAVLAERVGASVFINVTSVDGIYSADPKKDKKARRFE
                     TLTPKQLLAIVGGTALTAGSNTVLDIVAARVVERSHIPLVVLDGRDPKNLSRAILSGE
                     FHGTVVSEKKKKVLPL"
     gene            6210..>7079
                     /gene="amrS"
                     /locus_tag="VFC43_06150"
     CDS             6210..>7079
                     /gene="amrS"
                     /locus_tag="VFC43_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HZK30797.1"
                     /translation="MHEALRYEKTDNNAVKCSLCNYRCAILEGKSGICGVRRNEHGTL
                     YAMTYGKISAEAVDPIEKKPLFHYLPGTRSYSLGSIGCNFHCRHCQNWHISRAAFEDA
                     RLQTLLPETGVKRAIASGSASISWTYNEPTIWHEYALDMGTLARKKGLGTVYVTNGYI
                     TEEGLRELSPMLGAFRVDIKAFTDDFYKKVCGAHLQPVLDSAALARELGMHVETVTLV
                     IPGLNDSKEEMESLIRWVIDHLGPATPMHFSAFHPDYKMTDRGATPPQTLEKIYTLAK
                     GLGLRFPYLGNVYN"
BASE COUNT         1753 a         1786 c         1736 g         1804 t
ORIGIN      
        1 taaaaaggat cagcccaatt cttttaacca atgccgttca acattggtag agcgggctgg
       61 tagatcagtg gtagatcgct tccttggcat ggaagaggcc gcgggttcaa ttcccgccca
      121 gtccactcat ttctgaccgg acctatcatg ataaccggga tttttaatcc atcattttcc
      181 tgaaatatac cctatacgga tcgttttgtc ccacgatttt ttatacttgc ataccacagg
      241 gactattcag aacgattatg cgtatccgag atctgatcct ccccgaagac aaagtctttt
      301 ttactctctt taaagcgatg gcagaaaaga tagtcgaagc ttccactatc ttaaacgaaa
      361 tcactcatga gcttcccggt ggaacggaaa aggcgcacag ggttcgccag atcgagcacc
      421 aaggtgatga gatcacaagg cagatctacg aacagctcaa cgaatcactc atcacacctc
      481 ttgaacctga agagattgca cggctcgccc cggtactcga tgacgtgctg gataagatcg
      541 actgggtcac tcaccagctc tgcaactatg gcataccagc atcaaatgat gtactcaagg
      601 aatattccta ccttatccat ctcacgggca acgagatctc aaacgcaatc accaagctcg
      661 ccctgctcaa aaatccagaa gaggtaaaaa ctcatgccat agagattaac cggctctaca
      721 atctttcaac tgaactcctc tcacgggctg tcctggaact cttcaagacc aatgatctcc
      781 tgatgatcat caaactcaag gatatttacg aggggctggc aaaggttatg gaaacatgca
      841 acgatgtcgg acacgcactg aatgacatcg caatgagcca cgcatgatcg gcatggaacc
      901 cctcatcctg tttggcataa tccttgcact tgcactaaac tttgtcaacg gactcaatga
      961 tgcctcgcat tctatagcaa cagtggttgc gacgaaggca ctttccccga taaaagcggt
     1021 attttccact gcgatatgca atatgatagg tccttttctt ttttcaacgg cggttgccgc
     1081 tacgatcggt acggcaatag tcaccagaga tgcactcacc ccactctcaa ttgtcgttgc
     1141 aatgggggca tctatcatcc tggtcttcgt tgctaccagg atggggatcc cgatctccag
     1201 cagtcatgcg atggtggggg gattgctcgg agcggggatt ggtgcggccg ggttttctgc
     1261 ggtgatttta cccggagccg agaccgtgtt cgaggtaatt ttctatgcgt gtattggtgc
     1321 ggtcattgga gcactggtgc tatccattat ctgtgcagca ttcgaaggcg atatccggct
     1381 gggagtcctt atcggcggcg tctgcggggc agggatgatt atccccgctc tgatgattac
     1441 cggtgtgata catatccagg ggctgctcgc tatcgttctc ttcattctca tatccccgct
     1501 tataggtatg gccggtgcat ttctcttcaa tgtccttgtt tccaatctga tcaaacacgc
     1561 gaggcagaac cgtgcgaaac ggtttttcca gcccctgcat gtactcgcgt gccttgtcca
     1621 ggcaactgcc cacggggcaa atgacgggca gcatgcggtg ggggtgatca cagccctact
     1681 cctctcttca ggagcgttgc tcgtattcga cgtcccgcag tgggttatcc tcacttcagc
     1741 catcgcgatc ggtctcggta cctgttttgg cggctggcag gtagtagata aaatggcaaa
     1801 agagatcacc aagatccgcc cgtaccaggg tttctgtgcc gcaacagcaa gcagtgcgat
     1861 tcttgtgtta gtcacccacc agggtattcc cgtatcctcc acgcacgcga tcaacggggc
     1921 aattgtcggt gttggtgcaa cgagggggaa aaatgcggtc cagtgggaag ttgtccggga
     1981 aatgatgtcg gcatgggtaa tcaccatccc gctcgcactg gcagtttcat ggaccggttt
     2041 cctgatcatc agtgtagtgc tgaaactctt ttaaaaacca aacattattt cactatattt
     2101 tttaagatcc ttcaccgatt acctgtgggt ttgttatggg tcttcgtgag ttgctgatac
     2161 cgcatgataa gatttttttc gatatgtttg aaaaacaggc agcagttgtc aaagaggcgg
     2221 cctggcaact ggttgcattg actgaggatt tcaccagtgt caaggagaaa cgtcacgcaa
     2281 tcgagaaact tgaacataaa ggggatatga taactcacga tatctatgaa cagctgaacc
     2341 gcacgttcat cacaccgctt gatcctgagg agatctcccg cctcgcttca acccttgacg
     2401 aagtgctgga ttatattgac ggttctgcag agaaaatgtt ttattacaat attgaaggca
     2461 ccgactcaca catggttgaa cttgcaaaac tgatccacat gtccactgca gagattgaaa
     2521 gcgctgtcaa agggatccgt tcactcaaag acccgcggta tgttgaagag cgttgcattg
     2581 aagtaaaccg actggaaaat cttgccgatg acgtgcttgc ccatgcagta accgaccttt
     2641 tcaagaccaa tgatgcgatt acgattatca aactcaagga tatctacgag catctcgaaa
     2701 cggcaactga ttattgtgaa gacgtcgcaa atgtgctgtc cgatatcgcg atccgtcact
     2761 catgaacaca ttgcatacca gggataggag gattgcatga tcgaggcaac gtgggaaatc
     2821 atcatcttta tcataggcat tgcgctgatc tttgatttca ccaacggatt tcatgattct
     2881 gccaactcaa tttccactgt agtttcaacc aaggtcctct cacccagaaa cgcggtagca
     2941 ttcgccgcat ttttcaattt tattgctgcg ttcggtttcg gagttgccgt tgcaagcaca
     3001 atcagcaaaa ttataaaact ggatttcgtc cagaccgggc ttattcccta tattatctta
     3061 agtgccctta tcggagcaat tgcctggaat ctcattacct ggttcttcgg gcttcccact
     3121 tcctcctcgc atgccctcat cggcggactc tcaggtgctg gcatctccgc agccggtctc
     3181 gccgctatca aatggtcaac ggtcgaactg gttgcaacat tcatggtcat atcccctatt
     3241 atcgggctgg cctgcgggtt cctgttcatg gcagttgtcc tcaacctgac aaaaaaggca
     3301 aacaaacaga gtgcggaaat ttatttcaag cggcttcagc tcttatcagc agcagcctac
     3361 agtttcagcc atggtaccaa tgacgcgcaa aaaaccatgg gtattatcat acctcttctc
     3421 ttctcgatcg ggtattttgg ggcaggtgtc gatcccaatg ccctgccggt gccactttgg
     3481 gttatcctga ttgcccatgc tgccattgcc ctcggcactc tttcaggggg atggcggatt
     3541 gtaaagacca tgggctacaa aatcaccaaa ctccggccgg tgcaggggtt tgctgcggag
     3601 actgcaggtg cgacaacgat tatcggtgca tcccttattg gaatcccggt cagcacaacc
     3661 catattatct gttcctcgat tatgggcgta ggaacgacca tgggtgcaag tacggtaaaa
     3721 tggggtgtgg cccggagcat tgtaatggca tggatcctca ccattcccat cagtgcattg
     3781 atcggatttg tggcatttgc ggtaatacgg ataattattg gttactgacc tgataaatca
     3841 ggaatatttt ttttcatcgt tttccataaa aaatgtatta ttttatgatg aacttcaact
     3901 tcaaaacctc tcttcaggcc cggcggtccg acaggtttta attactgatc gcagatccga
     3961 caggagcggt atcagcaaaa attttggttg tttttgctcc aaagaaacgg agatgaacgg
     4021 gagttcacac ccgaagcatg aaaatcggtt ttctctacga tggacccccg cctcagggcc
     4081 tctctttagg cacggcgggc aaggattttt ttgagttagt cactgatccg ccgcgggggc
     4141 gccccgccgg gggcggcggc gttcatcgca tctgaagaat agatatgata attcccccat
     4201 catacctgaa tgattcgatt ttataggatc atcaattaaa aattctgatt cacttttggg
     4261 ctttcccctt gcacaggaaa tctgagttgc gatgacgatg tatgctgccc aacacgggtc
     4321 atcgcacttc gcgccgtccc accgcagggc atgggggtat aaggaggcat tattttgcca
     4381 ggaaaaaagt ttctccagag cagttatttt ttaaaagatg tcttgaaaaa ccggcaatac
     4441 ctgctgatcg gacagatttc acacctcggt ttctttgcat cgcagcactc ccgcccgtga
     4501 gagataagga catcggtcag atctccccat aactcctgcg ggaatgcggc catcaggtcc
     4561 ttctcaatca ccgtaacgtt atcagtgtcg gaaaatccga tccgctgtgc aagtctccgc
     4621 acatgggtgt caacggcaat tccttcattt tttcccagtg cgtggtagag cacgatgttt
     4681 gcagttttcc tcccaacgcc ggggattttt aatagttcct ccatggtctc aggtacatcg
     4741 cccccgaaat ctgacaacag ggcccgggaa gccaggatta tgttctttgc cttggcatgg
     4801 aaatacccaa gactgtggat aattttttcc acatccccga tgtcggccgc cgcaagatct
     4861 gaaggacggg ggtattttga aaaaagcgga tctctgactt tgatgactgc ccgatcggta
     4921 gtctgggcgg aaaggatagt caggataagg acttcaaagg ctgtgccttt tgagagcatt
     4981 gcaggagaat tgcgtgcatc aggatatcgc tgtaccagaa gggaatagat agtagcagca
     5041 tcctgttttt tcataaaaat gtcctttcag gcaacatacc actctcgtgc ttacaggaca
     5101 aaacaccgat gtgatttccc ggccataaat ggggtagggc ggattcgaac cgccgtcaaa
     5161 gcgtcccaaa cgctctagga tggaccaggc taccctacta ccccgtgcca tcactactgt
     5221 gccggcaaca taaaaatacg tagtggttgc cgtggcataa aaaataaaag ttacaggggc
     5281 aggacttttt tcttcttctc gcttaccacg gtaccgtgaa attctcctga aagaatcgcg
     5341 cgggaaagat ttttcgggtc acgtccgtca agcacgacaa gggggatatg gctgcgttcg
     5401 acaacccggg cagcaacaat atcgagcacc gtgttggacc cggcagtgag ggcagttccc
     5461 cctacgatag caaggagttg ttttggggtc agtgtttcaa aacgccgggc ttttttgtct
     5521 tttttcgggt ctgcagaata gataccatca accgaagtaa cattgataaa aaccgatgcc
     5581 cctacgcgtt ccgcgaggac tgctgcaacc gcatccgtag tctggccggg ggtaattcca
     5641 cccatgacaa cgatttttcc ggattcggca aacttttttg cctcaccatg actttctgcc
     5701 actttcgggt atgcccgctc acccagcgct gcgataagca ggtttgcgtt caggcgggtg
     5761 atcaggatac cgagctcatc agacgtccct tcatcgatcc cgagatctct ggcaacggtg
     5821 atatagcgcc gggcttcacc gcctccgcca acaacgacga acaggcggtg ctgtgcagca
     5881 atttctgata acacaggaat gtattctttg atcttattct tctcgagcag agggatcagg
     5941 attgagccgc ccagagacat gacaatggtc ttcattgcac tagtttttgt gccggatgct
     6001 catatagagt tgttgaatat gctgtactgt cctatctgta aaagccacga tatcttttcc
     6061 gtggcgggag ggtatatagg ccaggtgtat ctctgtaaga gttgtggata ccgggggtcg
     6121 tttgttcttg agactgatga tgagggtaca ggtcctgcag gagagaaaaa gcaataacca
     6181 ttgcctgcaa tagttcgatt gaaaagcgta tgcacgaagc cctccggtac gaaaaaactg
     6241 acaacaatgc agtgaaatgc tcgctttgca actaccgctg cgctattctg gaaggaaaga
     6301 gcgggatctg cggggtgcgc cgtaacgagc acggcaccct gtatgcaatg acctacggaa
     6361 agatcagtgc cgaagcagtg gatccgattg agaaaaaacc tctctttcac tacctgccgg
     6421 gcacacgttc atactctctg ggcagcattg gctgcaattt tcattgccgg cactgccaga
     6481 actggcacat ctcgcgggcc gcttttgagg atgcacgcct gcagacacta ttgccggaaa
     6541 ccggagtaaa acgggcaatt gccagtggaa gcgcaagcat ttcgtggacg tataatgaac
     6601 ctacgatctg gcacgaatac gcactcgaca tgggaacgct tgcccgcaag aaaggacttg
     6661 gcacggttta tgtgacaaac ggttacatca cagaagaagg gctgcgtgaa ctttcaccca
     6721 tgctcggtgc attccgggtt gacatcaagg ctttcacgga tgatttttat aaaaaggtct
     6781 gcggagccca ccttcagccg gtactcgatt ctgcagccct tgcacgggaa ctcggcatgc
     6841 acgttgagac cgtgacgctt gtcatccccg ggctcaatga cagcaaagaa gagatggagt
     6901 ccctgatccg gtgggtaatt gaccatctcg gcccggcaac cccgatgcat ttctccgcgt
     6961 tccatccgga ttacaagatg acggaccgtg gggcaacacc gcctcagaca ctcgaaaaga
     7021 tctataccct ggcaaaggga ctcggactcc gtttcccgta tcttggcaat gtatataat
//
LOCUS       DASRLV010000155         2635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1023097,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000155 DASRLV010000000
VERSION     DASRLV010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023097"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(335..616)
                     /locus_tag="VFC43_06155"
     CDS             complement(335..616)
                     /locus_tag="VFC43_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0058 family protein"
                     /protein_id="HZK30798.1"
                     /translation="MHKEELINLHQMLYDVKVYFEGLNPELKFSQYNALKITPSQQHK
                     SKMEHKHAIFVLGTEIANAMKEVDYSSSSRISARMKELAEKTLKEMESP"
     gene            complement(676..1908)
                     /gene="dnaG"
                     /locus_tag="VFC43_06160"
     CDS             complement(676..1908)
                     /gene="dnaG"
                     /locus_tag="VFC43_06160"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase DnaG"
                     /protein_id="HZK30799.1"
                     /translation="MYSPDTTKYLIHISLTAEGVVEKPDVVGAIFGQTEGLLGEDLDL
                     RDLQRTGRVGRIDVQITSKKGETKGEILISTSLDRAETAILAASLETIDRVGPCIAHV
                     VVESIEDIRVVKRKKIVERAKEILLDRFEDGTIDSDELLDDVRQSIRIEKIGSIGDEK
                     VPAGPNVQDSDAIIIVEGRADVLNLLRYGIKNAVAVEGTHIPRIVEELCEKKTATAFF
                     DGDRGGELILRELLQVADIDFVAFSPKNKSVEDMARKEVVKTLRNKVPVEYIRDQYSE
                     DSGEFPAEFRMSPVSQDDQGKTEKRSKQPAAVKKSAEGNKGPLTLRDHIDDVKGHSLA
                     RFLTEDLTVVQELKSDDLEKAVESVDSSVTGLVVDRAVDQKLLDQLVVKGLSYVAAKD
                     FKGIIKRPVSIRLMKMGS"
BASE COUNT          662 a          669 c          576 g          728 t
ORIGIN      
        1 agtcaccttt cggcaaagtg acatagagtt tgggaggcat ctgaacaagt cccctgccgt
       61 gatcagcaaa agccgattcc acggccatat ttacctgccc gatatccagg cccatatcgg
      121 tatcagtgaa atactgcatg agcgatcatc gatcgcgatc cgattttaac atatcttttg
      181 agaaaaacaa ttcgcaaaac cgcaatttct gttaagaaaa actcctcccc ccctctgttg
      241 cacgacaaaa tccagaatct ttttcgcgat ctgtactacg gtcaggaata gtaaacaaaa
      301 gggaaaaaat ggtacaggaa cggtgtcttt gaaattatgg agattccatt tccttgaggg
      361 ttttttcggc aagctccttc atccgtgccg atatcctgct cgaggaggaa taatccacct
      421 ctttcattgc attggcaatc tcggtcccca gaacaaaaat tgcatgcttg tgctccatct
      481 tactcttgtg ttgctgggaa ggggtaattt ttaatgcatt atactgggag aatttgagtt
      541 caggatttaa tccttcaaag tataccttca catcatagag catctggtga aggttgatca
      601 gttcctcttt atgcacacta ccactccgga cattaacgta gttggaatca acataaatac
      661 atatgcccgt ggttattatg atcccatttt catcagccgg atcgacaccg ggcgcttgat
      721 tattcccttg aaatcttttg cggcaacata cgatagaccc tttacgacca gttgatccag
      781 gagtttctga tccacagccc tgtcgactac aagcccggta actgaactgt caacgctctc
      841 gactgctttt tccagatcgt ctgatttgag ttcctgaacc accgtcaggt cctcagttaa
      901 aaaacgggca aggctgtgac cctttacatc gtcgatatga tcccggagcg tgagaggacc
      961 tttattgcct tccgctgact ttttaaccgc agccggttgt tttgaccttt tctcggtttt
     1021 accctgatcg tcctgtgaga ccggagacat gcggaactct gccggaaatt caccagaatc
     1081 ttcggaatac tggtcgcgga tgtactccac cgggacttta ttccgcaggg tcttgacgac
     1141 ctccttcctg gccatatctt ctacgctttt attcttcgga gaaaatgcga caaaatcgat
     1201 atcggcaacc tggagcagct cacggaggat cagctcgccc ccgcggtcac cgtcaaagaa
     1261 tgcagtggcg gtttttttct cacataattc ttctacaata cggggaatat gggtgccttc
     1321 gaccgcaaca gcatttttta tcccgtaccg cagaagattc agcacatccg ccctgccttc
     1381 aacaatgata atggcatccg agtcctgaac attggggccg gcagggacct tttcatcccc
     1441 gattgatcca atcttttcaa tgcgtatgct ctgccggaca tcatccagaa gttcatcact
     1501 gtcaatcgtt ccgtcttcaa aacggtcgag cagtatttcc tttgcacgct cgacgatttt
     1561 tttccgcttg acgacacgga tatcctcaat tgattccact acaacatggg ctatgcaggg
     1621 cccgacccga tcgattgtct caagagacgc ggcgagaatg gcagtctcag cccggtccag
     1681 cgaggtcgag attaatatct ccccttttgt ctcacctttt ttgctcgtaa tctggacgtc
     1741 tatacgcccg actcttcctg tcctctggag atcccgcagg tctaagtctt cgccgagtaa
     1801 cccttcagtc tgcccgaaaa tggcaccgac tacgtcaggt ttctcgacca ccccctctgc
     1861 tgtgaggctg atgtgaataa ggtacttggt agtatctggt gaatacacgt aaacgcaccc
     1921 ccatgaatca tacaatgaac ggaatgcctg atcataggta taaattatat atatcatgta
     1981 gaattactta aagtgtctgc tggcggtaat tatttgcgga aatatcggga acttgccgtc
     2041 gaaagaataa tttttttgct ctgtagaaac aggcatgaac ccatatgttc tcacccgaac
     2101 catgaaaatc gtacaatata cgaagggccc cgcctcaggg cctctccgag gcacggcggt
     2161 actgggaggt taattgtatt tagttactga tccgccacga gggagtccct tcggggcgtc
     2221 gtcgttcatc atagctgaag aatagagatg ataatccact cattatacct gattttttcg
     2281 attttcatgg gaaatcatcc tgaaaattct gatgttcttt ggggcttttc ccccccgcca
     2341 acggggcatc cccattgcga tgacgatgta ttacctgcta ttaaacccgg tttatcgtaa
     2401 tgcgcccgtg ccccaacggg gactaattgg cgcaaaaggg ggtattattt caacaaaaaa
     2461 gtattcaaca cagcaatttt ctcgttaaaa aactacaaga gtttttttat tgaaataatg
     2521 ctccctcttg cgctaattag tccccgttgg ggcacgggcg cattacgata aaccgggttt
     2581 aatagcaggt aatacatcgt catcgcaagg ggatgccccg ttggcggggg gggaa
//
LOCUS       DASRLV010000156         2969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1023145,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000156 DASRLV010000000
VERSION     DASRLV010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023145"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VFC43_06165"
     CDS             complement(<1..426)
                     /locus_tag="VFC43_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30800.1"
                     /translation="MRRASAVIIFMMKTMVFLSLGSPVTALDWTTETVDSPGDVGWYT
                     SLTLDNSGIPRISYFDWTKRHLKFAEKTGGMWTNETVDTTNAVGEFTSIKLDQSGQPL
                     ISYYDGNRGNLSFAMKNGEVWSTVIVDSGGVGRYTSLALD"
     gene            1410..>2969
                     /locus_tag="VFC43_06170"
     CDS             1410..>2969
                     /locus_tag="VFC43_06170"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HZK30801.1"
                     /translation="MKVWNLEKKGDEIIKISDTDGIYSFLVIGILFLLVWCAGCSEQT
                     DLGTQMPNPSVIPIQVKFVRGDIIAKNSSSADQFRLILKYDSLTGKYERASVRKKPDG
                     RWYRNNDTSEFSDRLLTEKIYSVKVGHVSSLSLVPVETPGSVPLATQEEKIPSTITTR
                     PTPSLSKIAPTAQFSASPLQGRVPLTVRFTDKSVFAGTTSYQWDINNDGVTDYTTKNP
                     VHIYQSVGIYTVKLTVTNASGSDRETKTNYITVSSLPSEETYGAEPNPTGNPLGGGAG
                     YSRIISETDSRVRHVVSTRDQLVTALKNAKPGEVVFVKGTAVIDMTGMPNSIIPGGVT
                     LASDRGNAGSQGALIKYSYNSKRGISLFEVGGNNVRVTGLRLEGEMLPQDGTGNGEAS
                     YLRAITVQNKAGLEVDNCEIRGWAWSCVTTMDSTGTFIHHNYMHHNQANGEGYGADIY
                     GGDMLVEANIFNYNRHDIAAGGYPGESYEARYNLVRGDGHPIGSHHFDVHACNPNDYS
                     DDSDTSPAFIAG"
BASE COUNT          824 a          703 c          669 g          773 t
ORIGIN      
        1 gtccagtgca agcgaagtgt atctcccgac tcccccgctg tctacaatga ccgtactcca
       61 gacctccccg tttttcattg caaaagacag gtttccgcgg ttcccgtcat agtaactgat
      121 gagaggttgt ccggattgat cgagttttat tgaggtgaac tcccccacgg cgttggttgt
      181 atccacggtt tcgtttgtcc acatccctcc cgttttttct gcaaatttca aatgacgttt
      241 tgtccagtca aaatagctga tccgggggat acccgaatta tcaagagtga gtgaagtata
      301 ccaccctacg tcgccagggc tgtcaacagt ttccgttgtc cagtccagcg ctgtcacagg
      361 tgatccaagt gagaggaaaa ccatggtttt catcatgaat ataataacgg ccgatgcacg
      421 tctcacaacc ccgttaaagg tctgatttgg agaaacccca tgatccgaaa tctggttcct
      481 gcagcaaaaa gaaagaattt tcccaaaaat atccatacgc catcacccct ttttctacgg
      541 ttcggggtga tgcggtattc aatcaggaca attttttaaa aaaatcatac tgaaatgttg
      601 gcagaacccg ttaatcagcg attttatgtg caaggttaat aacgttgtca tatatgctgg
      661 gaattttttt ataaccctga aaatcctgta tctttgatcc tttgatgatg agatggtctc
      721 gccagccctt ccttataata gacaaaaaat taagcatcac actggtttat gcaaaaagac
      781 gcaatgtaga attccttttg ccatgatatg aaatataaag tacctgtccc ctttaccacc
      841 gcccctgcgc tgaaagctat aatgatgtat tacctgtcca ggggttatcg tattcggggg
      901 agccacagcg gcgggagtga aacgcccctt gagtgtttag cttggtattg agcattttcc
      961 atgaaaatcg tattattgag atattgttac cctctctcca tctggtacga tgaatgcgcc
     1021 gcccccggag ggacgccccc gcggcggatt aatgaccatt ccaaaaaaaa aatccctgcc
     1081 cgctaggctc atagtggcct gaagcggggg tcttattacg attttcatag ttcgggtgtg
     1141 aactgaatct gttcatgtcc gtttctccag agcccagaaa caaacgtctc aaaacgggaa
     1201 attacaggaa taattgcaga gatctcccgt gactgcaaga aataattgtt caattaaaaa
     1261 aatctgactg tggagtacct cggtgagaat cctttctccc cggttttctc ttgcaaactt
     1321 attttgtata acaaaatata atatattctc aggatttcgt gtggaatctt tatccctctc
     1381 aaaaaatatt cgtttgattt tttcatatca tgaaagtctg gaacctggaa aaaaaaggag
     1441 atgaaataat aaaaatttct gatacggacg ggatctattc tttcctggtc ataggtattc
     1501 tttttctcct tgtatggtgt gccggatgtt ctgaacagac cgacctcggc acacagatgc
     1561 caaacccctc ggttatacca atccaggtta aatttgttag aggagatatc attgcaaaga
     1621 attcatcatc tgctgatcag ttccgattga ttctgaaata tgattccctg acaggaaaat
     1681 acgaacgcgc ctccgtgcga aaaaaacctg atggtcgctg gtatagaaac aatgacacgt
     1741 cagaattctc tgatcgcctc ctgacagaaa agatctattc ggtgaaggta ggtcatgtca
     1801 gctcattatc attggtgcct gttgaaacac ctggatccgt tccccttgct acacaagaag
     1861 aaaaaatccc atcaacgatt acgacccggc ctaccccatc tttatctaaa atagcaccaa
     1921 ctgcacaatt ctctgccagt cccctgcagg gcagggttcc acttaccgtc cggtttaccg
     1981 ataaatcggt ttttgcagga accacttcgt atcaatggga cataaacaat gacggggtta
     2041 cagattacac taccaaaaac ccggtccata tctaccagtc tgtaggaatt tacacggtaa
     2101 aacttactgt gaccaatgca tcggggagtg accgcgaaac caaaacaaat tatattacgg
     2161 tatcctcgtt gccatcggaa gaaacctatg gagcagaacc aaacccgacc ggtaatccac
     2221 ttggtggtgg agcaggttat tcccggataa tctcagaaac cgattcccgg gtgaggcatg
     2281 tcgtgtcaac cagggatcaa ctggtcactg cgctgaagaa tgcgaaaccc ggggaagtcg
     2341 tattcgtcaa aggcacggcg gtgattgaca tgaccgggat gccgaattcc atcatcccgg
     2401 gtggtgtaac tcttgccagt gaccgtggga atgctggctc acagggagcc ctcataaaat
     2461 attcctacaa ttcaaagcgt ggaatctcat tgtttgaggt tggaggaaat aatgtccggg
     2521 taaccggttt acggcttgaa ggggagatgc tcccccagga tggtacaggc aatggcgagg
     2581 ccagttattt acgcgcaatc acagtacaaa acaaggccgg gcttgaagtg gataactgcg
     2641 agatacgtgg ctgggcatgg tcctgtgtta cgaccatgga cagcaccggt acatttatcc
     2701 accacaatta tatgcaccac aaccaggcca acggtgaagg ttatggggct gatatttatg
     2761 gcggagatat gcttgttgag gccaacatct tcaactacaa ccggcatgac attgcagcgg
     2821 gaggataccc cggagaaagc tatgaagcac ggtataacct tgttcgcggg gacggacatc
     2881 ccatcggcag ccaccatttc gatgtgcatg cctgcaaccc gaatgactat tcagatgaca
     2941 gcgacaccag cccggcattt atcgcaggg
//
LOCUS       DASRLV010000157         3979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_465750,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000157 DASRLV010000000
VERSION     DASRLV010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465750"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VFC43_06175"
     CDS             <1..390
                     /locus_tag="VFC43_06175"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HZK30802.1"
                     /translation="VENELYCISGLNASDNEAMQDPHKKAYMQDLLSETEIAQKGVYR
                     LEASVKDLYNDYQAVASERVDKRLRLLTIVSAITLPLGLLAGLLGMNVGGVPGTTSGL
                     GFIMVTGLMLLVVIVEYWYFRHKGWFD"
     gene            914..3544
                     /locus_tag="VFC43_06180"
     CDS             914..3544
                     /locus_tag="VFC43_06180"
                     /inference="COORDINATES: protein motif:HMM:NF036590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HZK30803.1"
                     /translation="MANLLFRYYKTDFGKIPIRVVHMDITFDVYDDQTKVISDLHAES
                     RDSPVAEIALNAKNLEIIAVTCPGHESSFTYDTAGNLLTIRFSQPLAPLTRFTIHTET
                     ICHPTKNVLEGLYYDETPPGAPPQQITQCQQWGFQRLVPCIDDMTAKCTYTTTIIADE
                     RYTHLITNGNVVVPRQPAGPGRVKITYENIITPMAPYLFFLGCGTWETFSQECEYPDG
                     QRCSLELLVPPGSDPVLAGRALDILHDAVLWVYLFTGPDGFGKPEVRNRLYTLVTALN
                     LIKKTDPDSPQLPVIRAELSILIRQIVPGYAYTGTTYREIAMQNSDYGGMENVGNTTI
                     TANRIMPFPAMTDAGYEYLIRVKSHEFYHNLNGSEVTGWSPFEIWLNEAVTVHIENQY
                     HAFHFGEDYVRLQNVLSLLAPGSGTFDLDSGAASLPIEPDGFNDPNELITDITYMKAP
                     ELVKMIETCMGKEAFAKGLDLYHRRYRHGNATRAQWVEAMEEISGQDFTTMTEGWLKQ
                     TGFPTLSVQSSYDSKRRMMTLNLRQSCDDLQKHWIFPVRIALVSASGDDITEVLHVVK
                     NREETLEIVTPVKPAFLSINRGYSMYGKVRDETSSEELYLQAEHDHDLVNRCIAFVTL
                     MDREKLKMLEDPEALPDPACIDLYFRLLSDSDLMLSAGGQMVTLFESAPDKKYTNRYQ
                     DLYDVRERIFRAIAARYKQGLIDMCTAMNDPGILKDTPGEQARAIKQRQVKNTALSVI
                     ARHGTPDIHQMIKDQFDTASCATDKLVAFSLYMDSSAADRFDMLASFEQDSEKNPVSW
                     ENFLGVIAGNSSPEVMDLIARVERSATFRIEQANDQRALYGRFALNRKKSLQTEQGRA
                     FLHKTLRTLAPSTNTARLRC"
     gene            3547..3741
                     /locus_tag="VFC43_06185"
     CDS             3547..3741
                     /locus_tag="VFC43_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30804.1"
                     /translation="MFGTLDSMEESDQVPLTGILAGLLDDLDMEKTPSVYNTIRRLLA
                     GAPGAVRNYEQKHGIIKGIS"
BASE COUNT         1063 a         1029 c          994 g          893 t
ORIGIN      
        1 gtggaaaacg agctctattg catctccggc ctgaatgcca gcgataatga agcaatgcag
       61 gacccccata aaaaggcgta catgcaggat cttttatcag aaacggagat tgcacaaaaa
      121 ggtgtctacc gtcttgaggc cagcgtgaaa gatctctata acgattatca ggcggtcgca
      181 agtgagcgtg tggataagcg cctgcggctg ctgacaattg tctcggccat taccctgcct
      241 cttggtcttt tagcgggcct ccttggcatg aacgtcgggg gagttccggg tactacatcc
      301 ggtttgggat ttattatggt gaccggttta atgctccttg ttgtcatcgt tgaatactgg
      361 tatttcaggc acaaaggatg gtttgactaa acaggactaa aacaacgccg gtacagcata
      421 cgcgggagat gacacaggca aggacgatct gctggttctt tttacccgct catgtgaacg
      481 ggaaatgcac aggacaagcc ttgccgtgaa cagagcgttc attttagtta catgggatca
      541 ttaaggggtt tgtccggttg ccctgtgttc gtctgacacc ggctgatcac tgcaatgcaa
      601 tttcagcagg caaaccttcc ataattccag gttgtgcttt ttagcatcga atgaggggcg
      661 ggacccattt catgcattct aagaatttcg tttcataatt tgaaattcag acaatacgtt
      721 ttaaatccca gaattatatc acattcaggc tctgaacctt tcaatacaca ccaattcatg
      781 ccgtaatacc agactttgta tatggatagc aggatatgga catgacgtat attctggttt
      841 ttgggaagag aacaaatgca tacatacccc agatgagagc acgtttgaca aaaatataag
      901 gaaaaatact caaatggcta atcttctgtt ccggtattac aagaccgatt tcgggaaaat
      961 acccatccgg gtcgtccata tggacattac cttcgatgtg tatgatgatc agacgaaagt
     1021 aatatctgac cttcacgctg agtcccggga cagcccggta gcagagattg cactgaatgc
     1081 aaaaaacctg gaaattattg cagtcacctg cccgggtcat gaaagttctt ttacgtacga
     1141 tacagccgga aacctgctga caatccggtt tagtcagccg cttgcgccat taacacgttt
     1201 taccatccac acggaaacta tctgccaccc cacgaaaaat gttcttgaag gtctctatta
     1261 cgatgagacc ccgccgggag caccacccca gcagattacc cagtgccagc aatgggggtt
     1321 ccagcggctg gtcccctgta tcgatgacat gacggccaag tgtacctata ccaccactat
     1381 catcgcagat gagcggtata cccacctgat aacaaacggc aatgttgttg taccacgcca
     1441 gcctgccgga cccggaaggg tgaaaattac gtatgaaaat atcatcaccc cgatggcccc
     1501 ctacctgttc tttctcgggt gcgggacctg ggaaactttc agccaggaat gcgagtatcc
     1561 ggatgggcaa cggtgttcgc tcgagctcct tgtccctccg ggatccgatc cggtactggc
     1621 tggacgtgcc ctggacattc tccatgatgc cgttctctgg gtgtatctct ttaccgggcc
     1681 agacgggttc ggcaaaccgg aggtgcggaa caggctctac acactggtga ctgcattgaa
     1741 ccttatcaaa aaaaccgacc ctgactcccc tcaactgccc gtaatccgtg cagaactttc
     1801 cattcttatc cggcagatcg tccccggtta tgcatacaca ggtacaacct accgggagat
     1861 cgccatgcag aactcggatt atgggggcat ggaaaatgtc gggaatacca caataaccgc
     1921 aaaccgcatc atgccgtttc ccgccatgac cgatgccggg tatgaatacc tgatccgggt
     1981 caaatcccat gaattttatc ataacctcaa tggctcggaa gtgaccggct ggtccccgtt
     2041 tgagatctgg ctcaacgaag cggtcacggt ccatatcgaa aaccagtacc acgccttcca
     2101 tttcggagaa gactacgtgc ggctgcaaaa cgtcctttcc ctgctggcac ctggcagcgg
     2161 gactttcgat ctcgacagcg gggcagcgtc gctgcccatt gaacccgacg gcttcaatga
     2221 tcccaacgag ctgatcacgg atattaccta catgaaagcc ccggaactgg tgaaaatgat
     2281 tgaaacctgt atgggaaagg aggcatttgc caaagggctc gacctgtacc accgcaggta
     2341 ccgtcacggg aatgcgaccc gggcccagtg ggtggaggca atggaggaga tatccggaca
     2401 ggattttacc accatgacag aaggatggct caaacagacc ggttttccga ccctgtcggt
     2461 ccagtcatcc tatgacagca aacgccggat gatgacgctg aacctccgac agtcgtgtga
     2521 cgacttacaa aaacactgga tctttccggt caggattgcg ctggtatcag catcgggaga
     2581 tgatattacc gaagtgctgc acgtggttaa gaacagagaa gaaaccctgg agattgtcac
     2641 gcccgtaaaa cccgcattcc tgtcgatcaa ccggggctat tctatgtacg gaaaggtcag
     2701 ggacgagacg tcatcagaag aactttacct gcaggcagaa catgaccatg acctggtcaa
     2761 ccgctgtatc gcgttcgtga ccctcatgga ccgggaaaaa ttaaagatgc tggaagatcc
     2821 ggaagcactc ccggatccgg catgcatcga tctctatttc cggctccttt cggacagtga
     2881 ccttatgcta agcgccggcg gacagatggt aaccctgttc gaatcggcac ccgacaaaaa
     2941 atacaccaac cgctaccagg acctctatga tgtccgcgag agaatattca gggcgattgc
     3001 agcccggtat aaacaaggac tgattgatat gtgcactgcc atgaatgatc ctggtatcct
     3061 gaaggatacc ccaggggaac aggcaagggc aatcaagcag aggcaggtca aaaacacggc
     3121 actttcagtc attgcgcgac atgggacccc tgatatccac cagatgatca aggaccagtt
     3181 cgacacagca tcctgcgcta ccgataaact ggttgcgttc agcctgtata tggacagttc
     3241 agcagccgac cggttcgaca tgctcgcatc gtttgaacag gattctgaga aaaatcccgt
     3301 cagctgggaa aatttcctcg gtgttattgc cggaaacagc agcccggagg taatggacct
     3361 catcgcccgg gttgaacggt ccgccacatt ccgcatcgaa caggcaaatg accagcgggc
     3421 actctatggc aggtttgcac tgaaccgtaa aaaatccctt cagacagaac aaggacgggc
     3481 atttttacac aagaccttac gtacattagc accatcaacg aatacagcac ggttgcggtg
     3541 ctgagggtgt ttggcaccct tgattcaatg gaggaatcag accaggtccc gttgaccggg
     3601 atccttgcag gacttcttga tgatcttgac atggaaaaaa cacccagtgt ctataacacc
     3661 atacgacgct tacttgccgg ggcgccgggg gccgtcagga actatgaaca aaaacacggt
     3721 attatcaagg ggatctcatg aagatcctgc gtatatacca cccccgacgg gttgcccttt
     3781 aaaacgatat tttggctgat cttaaacgat gaaaacatga ggagaaccag acatgatcaa
     3841 agccattacc ccttgaagag taccgggccg tttacgaggc aagtcaggtt gagtgtccta
     3901 ctaccgagac catgagaccc accgaagaga ttatcgggca ggaacgggca caaagggcgc
     3961 tccggttcgg tcttgaaat
//
LOCUS       DASRLV010000158         4076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_466109,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000158 DASRLV010000000
VERSION     DASRLV010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466109"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..350)
                     /locus_tag="VFC43_06190"
     CDS             complement(120..350)
                     /locus_tag="VFC43_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30805.1"
                     /translation="MQDPGCYEKYPTRVIFVSNLLSLLIYGIGAYVLYRFGLIWVILY
                     IIFILVCEFRLLSGHCVDGYYYGKRCAFGKGW"
     gene            1497..2864
                     /locus_tag="VFC43_06195"
     CDS             1497..2864
                     /locus_tag="VFC43_06195"
                     /inference="COORDINATES: protein motif:HMM:NF005914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HZK30806.1"
                     /translation="MERGNPGPADVDGMESTVRSFLPDVIADLKELIKIPSVAFPGYP
                     PEPVHRMAQATVELLKRYGLSNARLMEIPGGYPTVIGDIPAPAGAPTVFLYAHYDVQP
                     ARREDGWETDPWIPFIKDGRLYGRGSADNKSGIATIAGAIRALSGRYPVGIKVIIEGE
                     EETASHLHAFVQAHPELFRCDLFIITDMGKMIPGEPVLSITLRGEVSCVVTVKTLSHA
                     VHSGVFGGPAPDALVALIRMLATLHDEHGDAAIAGLASYPWSGSEFPEPLYHRLSGLI
                     DGVDTIGSGTISSRIWSRPSVTVIGIDAPAVRDASNILIPQARARISLRIAPGADPVR
                     EVGLLCGHLKAHAPWHVCVEISEVHSSSGFICPTGGHGYEAAKKVMEAVYHRPVQEAG
                     AGGSIPLLNVLKQAVPGAEFVLWGCEDHEQSRIHGSNESVAVGELERMIITQALLLQE
                     MGKDP"
     gene            complement(2950..3192)
                     /locus_tag="VFC43_06200"
     CDS             complement(2950..3192)
                     /locus_tag="VFC43_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30807.1"
                     /translation="MYRCTREEKSIRSEVVLTGMAQLIIEPKKGKDGQIEFTVNYHDK
                     KSDNSFTITTTSDLDEAVEKLKATLVGEVANMQQKK"
     gene            complement(3211..>4076)
                     /locus_tag="VFC43_06205"
     CDS             complement(3211..>4076)
                     /locus_tag="VFC43_06205"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HZK30808.1"
                     /translation="GTLHFVAAPDIRWMLALLAPGIIAKSASRYRQLGLDEKTFAECK
                     QVLIHVLQGGRQRTRKDLISYLEDAGISTEGQRGYHLLRRSGLEGIICFGPKEDRQQT
                     FVLLDEWIPPSPGQDHLTPMAALAGRYIASHGPATLQDFIWWSGLSAADARSALAAAG
                     PDVYTDSIGGRVYWRSKSDFSSHDATPKTVLLSAFDEYLLGYRDRSHALDPGDAGKVN
                     LINGLALPILIDGKVAGTWKRTPKQKKVLIALDYFQYPKEDEQSAILAAAERYGEFIG
                     KSVELQHSYHP"
BASE COUNT         1016 a         1026 c         1006 g         1028 t
ORIGIN      
        1 gtacaatccc tgcgaggacc gggacgaaaa acagtaaaaa atccgggata atatctttcc
       61 ctgtgatcgt ttttttgcag aattgtttga gggtttcctt tgggaaataa cctgctgctc
      121 taccatcctt tgccaaaggc acaccgtttg ccataataat aaccgtccac acaatgcccg
      181 ctcagcaggc ggaattcaca cacaaggatg aagattatat agagaataac ccagatcagg
      241 ccaaacctgt acagaacata tgcaccgata ccgtaaatca ggagggacag gagatttgaa
      301 acgaaaataa ccctggtcgg atatttttca taacagccgg gatcctgcat accagaatag
      361 aataatgtaa aaaatatgat ataacttccg taaaccacgg tagtatcaga ttgacgcgga
      421 taacctcaga cgcaaatccc ttatggtcgt tgaaaattta caggtcatgt actgtcccca
      481 gcggtacttg caatgagcta aagagactgc gtgagaaaac ggcggtacag gaacgagagg
      541 ttcctgcgcc ccagaaagcg cggacgtaaa atatgaatcc gttcccacgc aacaacggcg
      601 tgaatgaaac gtgcctattc atttgccgta aatgacaatg gagagaatat gaatgtttca
      661 cgctgtattg tgcgatcatt tcttgttacc ggttcgttgt gcctgtagtt gttggcattg
      721 ccggagcgac cggcgctttt caggatcctg cccccgtgat caggggcatt accgggccga
      781 caagatgtac ctcaaccgat tatctgccaa cagaatgatt tcgataggtt caaaataata
      841 taaattccac aaacaatttg tgttgtttta atttatgcca tggactgctt gtacaatatg
      901 caaagggaga gctaaaatac cagatccgaa gcaaccagta tgggaaataa taaaattcgg
      961 acaaagatat attgggtgtc caaaacgcga ttgtctaaat ggcaaaattt tcatacgcaa
     1021 ccatgagtcg gtgcgttgtg atcgctatat accatcgagt catgagaagg ctaccaactg
     1081 aatgcaaacg agatattttc ctcctttttt gatctggatg cattccccat ctcttgagga
     1141 tatgtaagtg cagaatctct tctccttttt ttcctcctgg agaattttca ccgctgttga
     1201 gttgatcagc tcaaatcgta aggtcgttgt cagaactcca ttgatcgtcc tgtcttcttc
     1261 tcctggattt tgtctgctta atctcctgtg gtgagcgccc gcaactcatc tgcggaacga
     1321 ggaagtatct ctgtcatgtg ctcgctcaat gtacggctac ttcttctttt cttctttagc
     1381 cttccggtgc gttctgattt tcgcatccga actgagttaa tgtgcgttgt ggtccctttg
     1441 ctcggaagac aaccaaaaga gttcatctgt aaaaagatac tatatcggta tcagctatgg
     1501 aacgtggtaa cccgggtcct gctgatgtcg acggaatgga gagcacggtg cggtctttcc
     1561 ttcccgatgt tatcgccgat ctcaaagaac tcataaaaat accgtctgtt gcgtttcccg
     1621 gatatccgcc ggaaccagtc cacaggatgg cacaagcaac agtggaactc ctgaaacggt
     1681 acggtctgtc aaacgcccgg ctcatggaaa ttcccggggg ttatcccacg gttatcggcg
     1741 atataccggc ccccgctggc gcaccaaccg tgttcctcta cgcacattac gatgtccagc
     1801 ccgcacgcag ggaagacggg tgggaaactg atccgtggat cccgtttatt aaagacggtc
     1861 gcctgtacgg gaggggttct gctgataaca agtcaggaat tgcgacaata gcaggagcaa
     1921 tccgcgcatt atccggcaga taccctgtcg ggataaaggt aattattgaa ggagaggagg
     1981 aaaccgcaag ccatctgcac gcatttgtac aagcacaccc ggaactgttc cgatgcgacc
     2041 tcttcatcat cactgatatg gggaaaatga tccccgggga accagtgctg agcatcacgc
     2101 tgagaggcga ggtctcctgt gttgtcacgg taaagacgct ttcccatgcc gtccattcgg
     2161 gcgtatttgg cggccctgca cccgatgcac tcgttgcact catcaggatg cttgctaccc
     2221 tccatgatga gcatggcgat gcggctatcg ccggccttgc ttcgtaccca tggtcaggct
     2281 cagagtttcc tgaacccctc taccatcggt tatcgggtct tatcgatggc gtggatacca
     2341 tcggcagcgg cactatcagt tcccggatct ggtccaggcc gtccgttacg gtcatcggga
     2401 tagatgcacc tgcagtccgt gacgcatcaa acatcctcat cccgcaggca cgtgccagga
     2461 tcagcctgcg cattgcaccg ggagcagacc cagtgcgtga agtcgggctt ctttgcggtc
     2521 atctcaaagc ccatgccccg tggcacgtct gcgttgagat cagcgaggtt cacagttcat
     2581 cagggtttat ctgtccgact ggcgggcacg ggtatgaagc ggctaaaaaa gtcatggaag
     2641 cagtttatca caggccggtg caggaagccg gggcaggggg ttccattcct ctcctgaatg
     2701 tccttaaaca agccgtcccg ggagcggagt ttgtcctctg gggatgcgag gaccatgaac
     2761 aatcacggat ccatgggtcc aatgagagtg ttgctgtcgg tgaactggaa cggatgatca
     2821 ttacccaggc cctgctgttg caggagatgg gaaaagatcc atgagcatct aaaaaatcag
     2881 gcacggtact gcacccccaa aaaaaatttc agagtaactg cagcaaaaac cgttgttttg
     2941 ggaatttcct tactttttct gctgcatatt cgcaacttcc cctacgagag ttgctttcag
     3001 cttctctact gcctcatcaa gatcagaggt cgtggttatg gtaaacgagt tgtcactctt
     3061 cttatcatgg taatttaccg taaactcgat ttgtccgtct tttcctttct ttggctcgat
     3121 gatcagttgt gccatacctg ttaatactac ttcagatctt atgctttttt cttcccgagt
     3181 acaccggtac atcccgtctc atcgggagat tcatgggtga tatgagtgtt gtagctcgac
     3241 agatttacct ataaattcac catacctctc tgcagcagca agaattgcgg actgttcatc
     3301 ctcttttggg tattgaaagt aatccagtgc tatcaggact tttttttgtt tgggagttcg
     3361 tttccatgta cccgccactt tgccgtcgat aaggatggga agggccagcc cattgatgag
     3421 atttacctta ccagcatccc cggggtcaag ggcatgggag cgatccctgt acccgagcag
     3481 gtactcatca aatgcagaaa ggaggacggt ttttggtgtt gcgtcgtgag aggaaaagtc
     3541 cgatttcgat cgccaatata cccggccacc tatggaatcc gtatagacgt cagggccggc
     3601 tgcagcaagg gctgatcgtg catcagcagc tgacagacct gaccaccaga tgaaatcctg
     3661 cagtgttgcc ggtccgtggc tggcaatata acggccggca agtgcggcca ttggtgtcag
     3721 atgatcctga cccggggagg ggggtatcca ctcatccagg agcacaaacg tttgctgccg
     3781 gtcctccttt ggtccaaaac agattatccc ctccaatccg gaccgtctca gcaggtgata
     3841 cccccgctgc ccttccgtgg aaataccagc atcttcaaga taagagatca ggtctttgcg
     3901 ggtccgttgc cggccaccct gaagcacatg gataaggacc tgtttgcatt ccgcaaatgt
     3961 cttttcgtca agaccaagct ggcgataccg tgaggcactc ttcgcgataa tgcccggcgc
     4021 aagaagagca agcatccagc ggatatccgg tgcagcaaca aaatgcaatg tccccc
//
LOCUS       DASRLV010000159         5764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_302936,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000159 DASRLV010000000
VERSION     DASRLV010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302936"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..862)
                     /locus_tag="VFC43_06210"
     CDS             complement(11..862)
                     /locus_tag="VFC43_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HZK30809.1"
                     /translation="MGLINYNIEKYSPKQLVMIPLAMLAVALILLILNIATTGMPVTP
                     GIDFSGGTAVTIITTDTREQISLYFAEYPLSEVSDMNNGKFLKFGLMDDAKYKSLTSL
                     INQRYPDARIDQIGETFGKSLQSQAVLALIFSFIGMSIVVFLSFRTFVPSGAVVLSAF
                     ADIVMTAATMNLIGIQLSLGTLAALLMLIGYSVDSDILLTNRVLKRQGKLNEKLSGAF
                     RTGIIMTTTTLAAVSAMFLVSWFGAVQIIYEISAVLLIGLLFDVMNTWLTNVGILKWY
                     VLKGGGK"
     gene            1070..1381
                     /locus_tag="VFC43_06215"
     CDS             1070..1381
                     /locus_tag="VFC43_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2551 domain-containing protein"
                     /protein_id="HZK30810.1"
                     /translation="MRSPSEIKRVVEGRLRSYLSRDRTGIRREVLRLFVKIKSMTIAD
                     LVAELQTQFAVTFHAIASMVGIIASRIGILRATKNSQGVNNYELKEKYYDIVVRIVGV
                     S"
     gene            1436..1726
                     /locus_tag="VFC43_06220"
     CDS             1436..1726
                     /locus_tag="VFC43_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30811.1"
                     /translation="MRTNDSTCMSEVTIVPEVVDFINKRDCDFRICTSCGGPILLPVR
                     VKPPKKTDLLLRAGNHMIYVSIHQARFLHSIRMDMVPFFDDDDLVGMNTDEF"
     gene            1716..2123
                     /locus_tag="VFC43_06225"
     CDS             1716..2123
                     /locus_tag="VFC43_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HZK30812.1"
                     /translation="MSFEEISHTADIKIRVRAPSLEALFSETLNAMMQIIFGTDRGGY
                     IHKEIRIESSGNESLLTDFLSEVLFISEVENLVFSDADIKISGLHLSAELKGEQFDPL
                     RHSGGSEVKGISYSGLEITNDTNGYMLDIIFDV"
     gene            2151..3584
                     /locus_tag="VFC43_06230"
     CDS             2151..3584
                     /locus_tag="VFC43_06230"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HZK30813.1"
                     /translation="MLQGVNQVGVNEWEVPVGYVPGMRVPGKFFLSESLGRTLEPGAV
                     QQLANVATLPGILKNSLAMPDIHWGYGFPIGGVAAFSLEDGIISPGGVGFDINCGVRL
                     LSTPLQYKDVAGRRDLIDELFRTIPTGVGAKGFLKVSAPVLERMMVKGAKWAVSAGYG
                     IEHDLVRCEEGGFMKEADPHTLSSKAKQRGIPQGGTLGSGNHFLEVQVVREIFDPVAA
                     KAFGLTIGQVCCMIHCGSRGLGHQVCTDHLKTLESATKKYHITIPDRQLACAPIISPE
                     GIAYFGGMAAAANYAWANRQMITHTTRLVLEKMFGINYEEMPLIYDVAHNVAKMEEHA
                     IDGKRTKVCIHRKGATRSFGPGSADIPPDLETIGQPVIIPGSMGTSSFVLKGTQAAMD
                     RTFGSTCHGAGRIMSRTQAKKSLSGRDIMANLLEQGIIVRAHSENAITDEAPGVYKSS
                     DEVVNVVHEVGISRLVARLGPVGVIKG"
     gene            3581..4156
                     /locus_tag="VFC43_06235"
     CDS             3581..4156
                     /locus_tag="VFC43_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30814.1"
                     /translation="MNCRAGFVWQTKQHFNRYIEDCGISCELITPHMLAAPFYHGRFN
                     CLIIPTGFANPAYSNLLPALRAAAPRIKKFVENGGNLLVFGAAVAKPDSYDWLPFLVT
                     YTHDCHPRHIESVSDSGAMKIIDDYNPLCIECDGSFPTHEGQQVGIAGSADVIIETKV
                     GSGRIVVTSIHEYPSRGFLKNICSTGIESSF"
     gene            4406..>5764
                     /locus_tag="VFC43_06240"
     CDS             4406..>5764
                     /locus_tag="VFC43_06240"
                     /inference="COORDINATES: protein motif:HMM:NF014565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STT3 domain-containing protein"
                     /protein_id="HZK30815.1"
                     /translation="MGITLILRVIPAAFIKDAGFLYIFDTDSWYTMRQIEVMVRHFPQ
                     YNWFDPMTAYPTGKIIDWGPLFAGIAAIVCLITGATTRSAIIFTSGWVSPIIAVMMVP
                     VVYLLGKTLWNRETGIVAAGLISIVSIQFFSLSSYGWTDHHIAEVLFSSLFFLAYSFT
                     LSYMKSHQVSLKIPETLLLPVVYSGIVGVLFFLALLSSTTVILTLGVIAAYTLIQSVL
                     DFFSNRRTDSLLLINSTFLIVSIILLFLFGFKHEGVSLSRYTIGIVYVHLILLLGTIV
                     LFAISTILKGNRRAYLLTLLVLAAGTLVLTLIIPLFQTMLVQILGLLFGFSIFSVAVV
                     ETLPWTLTGAWENFNIALILMAGGFLWLGYTVLKKRDSQSVFLLIWSLFMLLLTIRFQ
                     RFAYFFTVNIVLLGAICITESIGLREDLFFKYWSGMVSPAPDSVHSPVNTGIDNPRKK
                     PVQ"
BASE COUNT         1553 a         1268 c         1316 g         1627 t
ORIGIN      
        1 tcttttgagt tcatttgcca cctcctttca ggacatacca tttcaggatc ccgacatttg
       61 taagccaggt gttcattaca tcaaagagga gtccgatcag caacacagct gatatctcat
      121 aaatgatctg gacagctccg aaccatgaaa caagaaacat ggccgaaacg gcagcaaggg
      181 ttgtggtagt catgataata ccagtacgga atgctcctga aagcttttcg ttgagcttac
      241 cctgccgttt aagaacccgg tttgtgagca ggatatcact atcaactgaa tacccaatca
      301 gcatgagaag tgcggcaagt gttccaagcg agagttgaat tccgataagg ttcattgtgg
      361 ctgcagtcat gactatgtcc gcaaatgctg aaagtacgac agcccccgat ggcacaaatg
      421 tccggaaaga taagaaaaca acaattgaca tcccaatgaa ggaaaaaatc aatgcaagca
      481 ctgcctgaga ttgcagggat tttccgaatg tttcccctat ctggtcgatc ctggcatcag
      541 gatatctctg gttaattaat gatgtgagtg acttatactt tgcatcatcc attaacccaa
      601 atttcaaaaa ttttccattg ttcatatcac taacttcact cagcggatac tctgcaaaat
      661 ataaagaaat ctgttccctc gtgtcagtag taataattgt tactgcagta ccaccggaaa
      721 aatcaatgcc gggagtaacc ggcatgccgg tagtcgcaat attaagaatg agcagtataa
      781 gagcaactgc caacattgcg agggggatca tcaccaactg ttttggtgaa tatttttcga
      841 tattataatt gatcaatccc ataacgtaat catctgttta caaatgatta aatagttacc
      901 agagggtttc ggataaaatg aaaaaacggt cacattctga agaacatttt tttaataaac
      961 ttcgcagaat atacattcgc cttcataaaa caacgagttc ttcatttaag caataaattt
     1021 tcgcacaaaa taaaaaaata tatatactga cagctttgac aataagtcta tgagatctcc
     1081 ttcagagatc aaacgggttg tcgaaggacg cttacggtca tatctctcac gggacagaac
     1141 cggaatccgg cgcgaagtcc tcagactttt tgttaaaatc aaatcaatga ctattgcaga
     1201 ccttgtcgct gaacttcaga ctcagtttgc cgttacattc catgcgattg cttcaatggt
     1261 agggataatc gcatcccgca tcggaattct ccgggcaacg aagaattccc agggtgtaaa
     1321 taattacgag ctcaaagaaa aatactatga tatcgtggtc aggatagtag gggtcagtta
     1381 accagcttcc gatccacaat tgtttttact gcaacattcc tattgttata gcatcatgcg
     1441 taccaatgac agcacctgca tgagcgaagt gaccattgtc ccggaggttg tggattttat
     1501 caacaaaagg gactgcgatt ttcgcatctg cacatcctgc gggggaccaa tactattacc
     1561 agtccgtgta aaacccccaa aaaagacaga tctccttctc agggcaggga atcatatgat
     1621 ttatgtttcc attcatcagg cgcggtttct gcacagtatc cggatggata tggtcccttt
     1681 tttcgatgat gatgatcttg tgggcatgaa tactgatgag ttttgaagaa atctctcata
     1741 ctgcagatat aaaaatcagg gttcgtgccc cctcacttga agcgcttttt tccgaaacat
     1801 taaacgcgat gatgcagata attttcggta cggatcgggg ggggtatatt cataaagaga
     1861 ttcggattga atcctctggc aatgaatcgc tgttaacaga tttcctgtcc gaagttcttt
     1921 tcatatcgga agtggaaaat cttgtctttt cagacgctga tatcaaaatt agcgggttgc
     1981 acctgtctgc tgaactcaaa ggagagcagt tcgatccact acggcactcg ggtggttcgg
     2041 aagtgaaagg gatctcctac tcgggactcg agattacgaa tgatacgaat ggatatatgc
     2101 tggacatcat atttgatgtc tgaaggtgca ggcaaagagg aggttggaga ttgctacagg
     2161 gtgttaatca ggttggagtg aatgaatggg aagtacctgt tggatatgta cccggtatgc
     2221 gggtcccggg gaagttcttt ctttctgaat cattaggcag aactcttgaa ccaggtgccg
     2281 tacagcagct tgccaatgtt gcaaccctcc cgggaatcct gaaaaactcg cttgctatgc
     2341 cagacattca ctgggggtac ggttttccca tcgggggagt tgccgcattt tctctcgaag
     2401 acggcatcat ttctcccggg ggtgtcgggt ttgatatcaa ctgtggagtg cggttactct
     2461 caacacctct tcagtataaa gatgtcgccg gccgcaggga cctgattgat gagctcttcc
     2521 gtacaatccc cacgggtgtg ggtgcaaagg gttttctgaa agtttctgca ccggttcttg
     2581 aacgtatgat ggtaaaaggt gcaaaatggg cagtcagtgc aggttatggg atcgaacatg
     2641 atctcgtacg gtgcgaggag ggaggtttca tgaaagaagc agaccctcat actctctctt
     2701 caaaggcaaa acaacgcggt attccccagg gcgggacgct tggatcaggc aatcatttcc
     2761 tggaagtcca ggttgtacgg gaaatttttg atccggtggc tgccaaggca ttcggcttaa
     2821 ccatcggcca ggtatgttgc atgatccact gcggttcacg ggggcttggt caccaggtct
     2881 gtactgatca tttaaagacg ctcgaaagtg cgacaaaaaa atatcatatc actattcccg
     2941 atcgacaact ggcctgtgca ccgattatat cgccggaagg gatagcgtat tttgggggaa
     3001 tggccgccgc ggcaaactat gcatgggcaa accgccagat gattactcat actacacggc
     3061 tggtactgga aaaaatgttc gggatcaatt acgaagaaat gccccttatc tatgatgtcg
     3121 cgcataatgt agcaaagatg gaggagcatg ccatcgatgg aaagcgaaca aaagtctgta
     3181 tacaccgaaa aggtgcaacc cgttcattcg gaccgggatc agcagacatt cctcctgatc
     3241 tcgaaactat cggtcagccg gtaataattc ccggcagcat gggaacatcg tcatttgtcc
     3301 tcaaaggaac acaggctgcg atggacagga catttggcag tacctgccat ggcgcaggca
     3361 gaataatgag ccgtacacag gcaaaaaaga gtctcagcgg cagggatata atggcaaatc
     3421 tcttagaaca ggggatcatt gtgcgtgcac acagtgaaaa cgccattact gatgaagctc
     3481 ccggcgtata caaatccagt gatgaggttg tgaacgttgt ccatgaagtc gggatatccc
     3541 ggctcgttgc gcgcctcgga ccggtcggag tgattaaggg atgaattgcc gggccggatt
     3601 tgtctggcag acaaaacaac acttcaacag gtatatcgaa gattgcggga tatcctgcga
     3661 acttattacg ccacatatgc tcgcggctcc cttttaccat ggaaggttca attgtctgat
     3721 tattccaaca ggctttgcta accctgcata ctcaaacctg ctgcctgcac tccgcgcggc
     3781 ggcaccaagg attaaaaaat tcgttgagaa tgggggaaat ctcctcgttt tcggtgcagc
     3841 tgtggctaaa cctgattcct atgactggct cccttttctg gtaacatata ctcatgactg
     3901 ccacccccga catattgagt ccgtgtcaga ttccggggct atgaaaatca ttgatgatta
     3961 caacccccta tgcatcgagt gcgatggatc atttccaacg catgaaggac agcaggtagg
     4021 aattgcggga tcagccgatg taattattga aacgaaggta ggcagcggga gaattgttgt
     4081 gacaagtatc catgaatacc cttcacgcgg ttttttaaaa aatatctgca gtacgggaat
     4141 agaaagttca ttttaaaaaa aataacaaaa cagtttttaa atcaagtatc atggtcgttt
     4201 gactggagct gactaaaaaa agcagaaaaa ccatttttcc atggtttgtt ttttgtgatt
     4261 ttgcagcatc tattatcata caggattaaa aatccgatat gttgttgaag gtttattata
     4321 attatatcct tagtatgtat taatggattt aattcatctc aaaaaacatc agccttacat
     4381 cattgttgcg ttactccttt tttttatggg aattaccttg attctccggg ttattcctgc
     4441 agcatttatc aaagatgccg ggtttttgta tatttttgac acggattcct ggtataccat
     4501 gaggcagatt gaggtcatgg tcaggcattt tccccagtac aactggtttg atccgatgac
     4561 cgcttacccg actggaaaaa taattgactg gggtccgctc tttgcgggta ttgcggcaat
     4621 agtgtgcctg ataactggtg ccacgacccg aagcgcgatt atttttacct cgggctgggt
     4681 atctcccatc atagccgtga tgatggtgcc ggtagtatac ctgctgggaa aaacactatg
     4741 gaaccgggaa acaggaattg tcgctgcagg tctcatttct atcgtttcaa tccagttttt
     4801 ttccctttca tcctatgggt ggaccgatca tcatatcgca gaagtccttt tcagttcgct
     4861 tttttttctt gcctatagtt ttaccctgtc gtatatgaag agccatcagg tttccctgaa
     4921 aattccagaa acgcttttgt taccagtcgt ctactcaggg attgttggag tgttattttt
     4981 cctcgccctc ctctcttcaa caaccgtgat ccttaccctg ggggtcattg ctgcctatac
     5041 ccttattcag tccgttcttg attttttctc gaaccgccga accgattcac tgctgctcat
     5101 taacagtact tttttaattg tctcaataat cctgcttttt ctcttcggtt tcaagcatga
     5161 gggagtatcg ctctcacggt acaccatagg gatagtctat gtgcacctga tccttctcct
     5221 cggaacgata gttctgtttg ccatttcaac aatccttaag ggaaatcgac gggcgtatct
     5281 gttgactctg ctcgtacttg cggctggaac ccttgtttta actctgatta ttcctctttt
     5341 ccagacaatg cttgttcaga tactgggact tttattcggt ttttcgatct tctctgtcgc
     5401 agtcgttgaa acccttccct ggacactcac gggcgcatgg gaaaatttca atatcgcgct
     5461 gatcctgatg gccggaggat ttctttggct tggatacacg gttctgaaaa agagagacag
     5521 tcaatcagtt ttcctgttga tttggtcctt gttcatgctt cttctgacca tcagattcca
     5581 gcgtttcgca tattttttta ccgtaaatat tgttctactc ggtgcaatct gtattactga
     5641 gagtattggc ttgagagaag accttttttt caagtactgg tcaggaatgg tatcccctgc
     5701 accggactct gtacattctc ccgtcaacac aggaattgat aatcccagga aaaaaccggt
     5761 acaa
//
LOCUS       DASRLV010000160        16398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1114660,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000160 DASRLV010000000
VERSION     DASRLV010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 16398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16398
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114660"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /gene="dapB"
                     /locus_tag="VFC43_06245"
     CDS             complement(<1..568)
                     /gene="dapB"
                     /locus_tag="VFC43_06245"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HZK30816.1"
                     /translation="MIKVVVCGASGRMGQTIGRMVNESSDLELVGGINLKPGSFFGVD
                     IVASDNAENLLRKTRPDVLIDFTVASAAVGNVKMAARNKVALVVGTTGVTHEQLREMD
                     TAIQGHVPAVISSNFSLGVNIFWQILRNTAKLLKDYDIEVIEAHHRNKKDAPSGTAKT
                     ILQILDEEVGSRKKMYGREGMMERSDEIG"
     gene            complement(577..1545)
                     /gene="dapA"
                     /locus_tag="VFC43_06250"
     CDS             complement(577..1545)
                     /gene="dapA"
                     /locus_tag="VFC43_06250"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HZK30817.1"
                     /translation="MFEGVLPAIITPFKRNSAMGLDIQGLERNIGYLLSCGIHGIVPC
                     GSTGESATLTFEEHEKVIEIAVNKVNGRIPVVAGSGSNNTAEAVRLTKAAKDIGADGV
                     LVISPYYNKPNRSGLIKHYTKLADLDIPVIMYNVPGRTGQNLEPDLVAELAAHPNIVA
                     IKEASGNIGQISRIIEETQDDDFLVISGDDNITLPIMALGGAGVISVAANVDPKRMVA
                     MYEAMKKGDNQKALVLHYALSPLMRSMFIDTNPIPVKKAVELLGMAGGPVRLPLDDLD
                     DKKTEQLKKILATIPGKSKKTTPDVPKKPIKKVIVKTKTTDKKKVR"
     gene            complement(1554..1745)
                     /locus_tag="VFC43_06255"
     CDS             complement(1554..1745)
                     /locus_tag="VFC43_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17e"
                     /protein_id="HZK30818.1"
                     /translation="MGIKPSYIKSLGTELLAKQRDYFTNSFDENKQQLGKSAVIGSKR
                     VRNRVAGYITRKINTKRRS"
     gene            complement(1756..2313)
                     /locus_tag="VFC43_06260"
     CDS             complement(1756..2313)
                     /locus_tag="VFC43_06260"
                     /inference="COORDINATES: protein motif:HMM:NF021625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate synthase family protein"
                     /protein_id="HZK30819.1"
                     /translation="MKNPDRERSDALEKLEMAAAILEQSMDVRLIPVEGTKFGYAIRG
                     ARDKEGVAAVQDGITQKNKMPHITGPCAFGVDEDISRMILTTMKFDPRMRCAATLRFS
                     GKVLGIIESLLLECSSFDPASAQPGISTMDWGVASCCKEGVPDVCFDTGGKGKEAIIR
                     LFGEEPNDVSNNIIMISNRIISIEL"
     gene            2418..2594
                     /locus_tag="VFC43_06265"
     CDS             2418..2594
                     /locus_tag="VFC43_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30820.1"
                     /translation="MDPRLLDVIIGVSGFLVLIVMLALLPMVMMSGIAYIAAIVIFIL
                     FLSGAGYLVNEKII"
     gene            complement(2587..3693)
                     /locus_tag="VFC43_06270"
     CDS             complement(2587..3693)
                     /locus_tag="VFC43_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase large subunit PriL"
                     /protein_id="HZK30821.1"
                     /translation="MDYQPSPKELSHFPFLKNAQDNIKDVFPPLDVLLKQERGKIIID
                     LALKRINQALSSKKTFIAHFQQRDDDEIASFVLSRIIVSCIQDKQIFDRLTRYEAERA
                     YYFLVTELGSEDGERGWNENILLDDEGYSPIAEYLAAEFRINIAEEHMPLIDYIEIAA
                     PLHDERFKLVNRGVQQGFVAIKKEERYELLRERIRVILRRDLPYKIPKSLCEQLLPLT
                     DPIKKEYQEKMLQQFGSIEESAFPPCMQALILALTAGTNLSHAGRFALTTFIHTIGMN
                     VMEIANLYARSPDYDPEKTMYQVEHITGRGGTGTEYTTPACAAMKTTGLCTHKDPLCE
                     KISHPLSYYKWKKKDQSKKKQESPFKNTAPSSPR"
     gene            complement(3693..4436)
                     /locus_tag="VFC43_06275"
     CDS             complement(3693..4436)
                     /locus_tag="VFC43_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase sliding clamp"
                     /protein_id="HZK30822.1"
                     /translation="MLKATIDADMFREFIDAISALVPECRLHTDENGISTRAVDTANV
                     AMIGIELKKEAFDSYKATKSQLGIDISKMKNIFGMAGKGDLISLELADNAQKMSVSAH
                     GYHYSITLLDTNTIRKDPNPPTINLPGKIVISGDDFNNAMKAAAVISDKIALGINPKE
                     QTFFLIAEGDTDNIRREFSKDELKSLTPVEARSLFSLDYLKDMGKVMSKADEVEIFLG
                     IDHPVRFSFVIAGGNGHVEYLLAPRIEAD"
     gene            complement(5043..5711)
                     /locus_tag="VFC43_06280"
     CDS             complement(5043..5711)
                     /locus_tag="VFC43_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HZK30823.1"
                     /translation="MQLTWLGHACVLLAGSKKILIDPFIEGSSVLYTRPDIVAITHGH
                     SDHMGEAVALGKKTIAITEIARYLMTRDIAAEGMNIGGTIIVDGVSFTMTPAVHSSSI
                     EEAGMGYYGGSAAGYVIGMDGLKIYHAGDTALFSDMNLIRELYHPDVALLPIGGRYTM
                     GISEAMIAANFIGAKTVIPIHYNTWDKITADPVKFREAVERTTDMKVKVLKPGESMEI
                     SITK"
     gene            complement(5823..6431)
                     /locus_tag="VFC43_06285"
     CDS             complement(5823..6431)
                     /locus_tag="VFC43_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator receiver protein"
                     /protein_id="HZK30824.1"
                     /translation="MTEDKRKVQLLKLFSTMSGKTKIVEPMKNIHGTLRDSDAIEREV
                     ALVMREITEQGIFKTSLKPIQLAKLVTLFYAGKNDTEIARQLGDEKLSKTVARARVRL
                     KLFRELDFKMPFDKLKMEQLIESGKTMKEVSEELGISPSTLREYRHVIEEQEDPTIDP
                     YIDRVRDVMEDRDISEQMTRSATADSLGDSIDITEAELIDVM"
     gene            7060..8760
                     /gene="glyS"
                     /locus_tag="VFC43_06290"
     CDS             7060..8760
                     /gene="glyS"
                     /locus_tag="VFC43_06290"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HZK30825.1"
                     /translation="MDLAKRRGFIWPTSECYGAVAGFIDYGPLGAMMKRRVENIWREF
                     YVIREGHYEIECPTIAQEAVFIASGHVKGFSDKMCQCPHCKEYLRADHVAEAGNIHNA
                     STMKNEDLAAAITSYICPACNEVLGNVAVFNFNLMFSTTIGPGSQRIGYLRPETAQGM
                     FVDFSRLLRFYRDKLPFGAVQIGKSYRNEISPRQGMIRLREFTQAEAEIFVHPDEKNH
                     HPSFKRYADYSMPLLTYIQQQNCEDPVEITMREAVDRGIIANEYVAYYVALTHDLLVT
                     IGIKPERLRFRQHLPDERAHYATDCWDAEVFSPRFGWVETVGIADRTDYDLKAHSGQS
                     GTPMTVFIQYEEPKKTERRRIIPNMSVLGKQYRVKAKSVFAALEHATPTPDGADVEVD
                     GEKIHIPADLFEVRDEVIEVRGEEIVPHVIEPSYGIDRMCYAVLEQAYDEDIADGEYR
                     TVMRLSPNIAPVQVAVFPLMNRDGLDTLAKDIITSFHARGILAEYDDSGAIGRRYRRQ
                     DEIGTPFAITVDYESKENNTVTLRNRDTMKQVRIAITDLPGTVDALVKGNIPFASLTP
                     "
     gene            8770..11007
                     /locus_tag="VFC43_06295"
     CDS             8770..11007
                     /locus_tag="VFC43_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZK30826.1"
                     /translation="MSFITHPLIKPESIDSREYQLSIAMRALDANTMVILPTGLGKTA
                     VALLVAASRVYNEGGRVLMLAPTKPLVEQHLRYFERYLLLKSREGSDISPFVMFTGEA
                     PPDQRTREWNNATVILATPQVVKNDLIACRYTLKDVSLLIVDECHRAVGNYAYVFLAQ
                     RYLATADKPLLLAMTASPGGILEKVEEVSSNLGITHVENRTEHDPDVRPYVFERDIDY
                     ISIDLPPELRVSIKLINTCIEERLALLASLHFTVPKREKLTMKALNELNAQIQQRIAN
                     RDPVAYSAASLYAACMKLRHAITLAESQGSEVLKGYLAKLVAEGTGSGGSKASQGLVK
                     DPAFHELFERSLQWTDELHPKPGFTLTLVRDQLESHQDSRIIIFATYRDTVQLLVDHL
                     TKNGIACERFVGQATKDAEKGLTQKKQIAILSRFREGEFRVLIATSVGEEGLDVPSTD
                     LVIFYEAVPSEIRSIQRKGRTGRSRAGRVIVLVTKGTSDEVYRHVSQAKEMMMHKSMR
                     EIVRTNALQQQPVLPQQASIDEFTVQGPGIIIDDRETSSKVVEVLSGLGADIRLKRLP
                     HGDYAIGDRILIERKTARDFVDTLINRDLLGQVKAMAEVVPRPVMIIEGGDLYTQRDI
                     HPNAIKGVLAALTIDMGVSLMFTRDEQDTAQMLFILAKREESERGERKVHPHKSHRSV
                     REDQEYIVSAFPEVGMKNARLLLSHFGSIRAITDAGLPELTAIKGIGEKTAQKIFDLC
                     RHSYN"
     gene            complement(10998..11384)
                     /locus_tag="VFC43_06300"
     CDS             complement(10998..11384)
                     /locus_tag="VFC43_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sjogren's syndrome/scleroderma autoantigen 1
                     family protein"
                     /protein_id="HZK30827.1"
                     /translation="MPVRKEDEIMAEYLLKGGKMLEKSCKTCGCPLFEVKGKTLCVVC
                     AENEVVKKPGPSTAAPVHQEHDQECSCGEHHEETCEGGLLTDELAMTIVSLCERIQNE
                     KDPENVLALMNAVKAGTEALQILCQL"
     gene            11464..11727
                     /locus_tag="VFC43_06305"
     CDS             11464..11727
                     /locus_tag="VFC43_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0147 family protein"
                     /protein_id="HZK30828.1"
                     /translation="MPNPEKTMENCILMLQRIQEDSSIPRNIRRVADETRTLLTNNTK
                     AMGLRAAEAISKIDEISNDPNMPIHARTRIWELVSQLETIPLD"
     gene            complement(11804..13564)
                     /locus_tag="VFC43_06310"
     CDS             complement(11804..13564)
                     /locus_tag="VFC43_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZK30829.1"
                     /translation="MISLLYVDDEPELLDLCKIFLEKEGEFSVVTVTSAKEGMQILSE
                     GMIDAVVSDYQMPFMDGIEFLKQVRMKFDDIPFILFTGKGREEVAVQAINNGADFYLQ
                     KGGDPRAQFSELSHKVRQAFRRKKAEEELRMMKATVSNAAEGILWINENGGITFFNDT
                     ICSMLGYLREEFTSLSIRNINAEFMENHWGSLWAGMKEKRYITVQDSMRKKDGSVIPV
                     EILFNYIEFGSLAYIFAFVRDITDRKRAEDELKSAFEKTKGLMDHANDAIFISDQETG
                     VLLDANIKAQKMIGKTLAEIHTMHESELFPVENKEKYHEYFKKHAQEGTGLQAEVVNH
                     KGARIPVIVSATILDLGGRQSMMGIFHDISDIRKTQDALQLANKKLNLLAEITRHDIR
                     NKLTVLGGYLELFNDHPPEPQHSMYLEKLKAAVRMITENIEFTKLYQDLGVVAPNWKN
                     VSDTFYRACVHIDIKKIRFESSVDGLEIFADPLLDRVFYNILQNAVEHGDHVSVVKMS
                     SVELPDGLLIRIEDDGIGIPSHDKETIFVRGFGKNTGLGLFLCREILSITDITIKETG
                     EFQHGALFEMHIPKGVYRHT"
     gene            complement(13778..16138)
                     /locus_tag="VFC43_06315"
     CDS             complement(13778..16138)
                     /locus_tag="VFC43_06315"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HZK30830.1"
                     /translation="MISVLYIGNDTAFLEAGKRFLEHDADIRVQTTQSAVDALNILKT
                     ASYDAVIADYDKPDDGIQVLRSVREKFPKLPFIFFTDKKREEFLAEAFKNGVDHYVKK
                     GGDQTSQFAELSHVLRNAVEFRGNEQKIALLNRIDALHRRINEAVVHIHDRRQLAQEV
                     CRIMIQEGGFVLAWIGFEDPVTARIDTVFASGETDEFFGKVRLFSEDISQGHGPTVTT
                     IKKGKYTICNDIQAFPDLQMWEDDAIRKGYRSAAAFPLRSGKATRGAITLYSVENNFF
                     TDTEIRLLNDISQEISWVFITMDMLDSSRQVQGDLELSEHRLTQIINFLPLATFATDT
                     AGFIIIWNKAMEKLTAVSAEQVTARGDYEYSFRILGERIPGLLDLVFASDTEIENYHY
                     SALQRTRGCVRAQINVPDLRGKPATLEAVASLLYDQNGQLSGAIETLTEIGNMHRKDE
                     KFHHLFQTAEYGLLILDPDTKKIIEANSWINSLTGYSREYLIGKKIEDAGFFKDASGA
                     DLFFTELKKSDHVHYGDIPLVTKDGTSVDVEFFSRITSFDDRQIIQCSVYSISKRKRN
                     ETAQKIARKNLDVFSSRIRHDILNQLMIVSGSLELASYSVQDPDLQKHLNRAQTATKT
                     IQRQILFTREFEDLGAEIPTWQIVPTVIHHAFLDIETDSITLNVQQDEIVVFADPLFE
                     KVFYLLFDFAHRFGEKVTRIDISYSFTPEELIIVVSDNGIGIVPETKDQLFEWRPGND
                     KNAGLHLAQKILSSTGLSIRETGEFRKGARFEIIVPKDAFHFGSSR"
BASE COUNT         4149 a         3862 c         3712 g         4675 t
ORIGIN      
        1 ctccaatctc gtctgagcgt tccatcattc cctcacggcc gtacatcttt ttccgggatc
       61 cgacttcttc atcaagaatc tgaagaatcg tctttgcagt tccgctgggg gcatccttct
      121 tgttccggtg atgtgcttcg atcacctcga tatcataatc ttttaagagc tttgcggtat
      181 tccgcaggat ctgccagaat atattgacac ccagtgaaaa gttactggag atgaccgcag
      241 gaacatgtcc ctggatcgct gtatccatct ctctcagttg ttcatgcgta actccggtag
      301 tgcccactac cagtgccact ttattccggg cagccatctt tacattcccg actgcagcac
      361 ttgcaacagt gaaatcgatg agtacatcag gtctggtttt ccgcagaagg ttttctgcat
      421 tatctgatgc aacgatatca accccaaaga atgaaccagg tttaaggtta attccgccaa
      481 ccagctcaag atcggatgat tcgttgacca tacggcctat cgtctgtccc atgcggccgg
      541 aagcgccgca tacaaccact ttaatcatgg ttcaactcac cggacctttt ttttatcagt
      601 tgtttttgtc ttgacaatta ctttttttat aggttttttt ggtacatcag gagttgtttt
      661 tttagatttg ccggggattg tggcaaggat ttttttgagc tgctcggttt tcttatcatc
      721 aaggtcatcg agcgggagtc tcactggtcc acctgccatg ccaaggagct caactgcttt
      781 tttgaccgga atcgggtttg tatcaatgaa catcgaccgc atgagcggtg acagagcata
      841 atggaggaca agcgctttct ggttgtcgcc ctttttcatt gcctcgtaca ttgcgaccat
      901 acgtttaggg tccacattag ctgcaactga gatgacaccc gccccgccga gcgccatgat
      961 ggggagcgtg atgttgtcat caccggaaat cactaagaaa tcatcatcct gcgtttcttc
     1021 aatgatccgc gagatctgcc cgatgttgcc acttgcttcc ttgattgcaa caatattcgg
     1081 gtgtgccgca agttccgcga caaggtcagg ttcaagattc tgtccggtcc tgcccgggac
     1141 attatacata atgaccggaa tatcaaggtc agcaagtttc gtgtaatgct tgatgaggcc
     1201 tgaccggttg ggcttgttat aatacgggct gataacaaga accccgtcag cgccgatgtc
     1261 cttggcagcc ttcgtcagcc tcacagcttc tgcggtgttg ttggaaccgg atccggcaac
     1321 aacagggatc ctgccattga ccttattaac ggcaatttca ataaccttct catgctcttc
     1381 aaatgtgagc gttgccgatt caccggttga tccgcatggc actatcccgt ggatcccaca
     1441 ggagaggaga tacccgatat tgcgttccag gccctggata tccaggccca tcgcagaatt
     1501 ccttttaaaa ggggtaatga ttgctggaag gacaccttcg aacatgaata agtttatgag
     1561 cgtctcttgg tatttatttt tctcgtgatg taaccggcga cacggttccg gactcgcttg
     1621 cttccaatta ctgcagattt tcccagctgc tgtttgtttt catcgaagct gttagtgaaa
     1681 tagtcccgct gctttgccag aagttcagta ccgagagatt tgatgtatga cggtttgatt
     1741 cccatgtgca tttcctcaaa gttctatact tataatgcgg ttcgatatca taataatatt
     1801 gttcgatacg tcgtttggtt cttcaccaaa aagacggata attgcttctt ttccttttcc
     1861 gccggtatcg aaacaaacgt cgggtactcc ctctttgcag caggatgcaa ctccccaatc
     1921 catggtactt atcccgggtt gtgcggacgc gggatcaaac gaagaacatt caaggagcag
     1981 ggactcaatg atacccagta cttttcccga aaaccgcagg gtggctgcac acctcatacg
     2041 cggatcaaat ttcatggtgg tgagtatcat tcgtgagatg tcctcatcta cgccaaatgc
     2101 gcacgggccg gtgatgtggg gcattttgtt tttttgggta atcccgtcct gaactgctgc
     2161 aaccccctct ttatcccgtg cgcctcggat cgcatacccg aattttgtcc cctccacagg
     2221 gataagccgg acatccattg attgttcaag aattgctgca gccatttcca gtttttcaag
     2281 cgcgtcactg cgttcccggt ccgggttttt cataatcata ccattgatgg acatgacgcg
     2341 taataagggt gtcttttcct taaatgccgt ctcctttttg gatatacata tatcctttta
     2401 cgatgaagat tcctgtaatg gatccacgac ttcttgatgt gatcattggt gtttcgggct
     2461 tccttgtcct gattgtaatg ctcgccctgc tccccatggt gatgatgtca ggaatagcct
     2521 atattgcagc tatcgtaatt tttatcctgt tcctttccgg cgcgggctat ctggtgaatg
     2581 agaagattat ctaggtgagg aaggggcagt atttttaaag gggctctcct gtttcttctt
     2641 tgactggtct ttctttttcc atttgtaata actcagagga tggctgattt tttcacataa
     2701 tggatcttta tgtgtgcaga gccctgttgt tttcatcgct gcgcatgccg gtgtcgtgta
     2761 ttcagtgcca gtacccccgc gcccggtaat atgttcgacc tggtacatgg ttttttctgg
     2821 atcataatcc ggcgacctcg cgtagagatt ggcaatttcc atgacgttca taccgatagt
     2881 atgaataaag gtagtcaggg cgaatcgtcc tgcatgggac aggtttgttc ctgccgtaag
     2941 agctaaaatc agtgcctgca tacatggggg aaatgcactt tcctcgatac taccgaactg
     3001 ctggagcatc ttttcctggt attctttttt tatgggatcg gtcagtggca ggagctgttc
     3061 acagagcgat tttggaattt tatatggaag atcgcgacga agtatgacac gaatccgctc
     3121 gcggagtaat tcatatcgtt cttctttttt aatcgcaaca aatccttgct gcacgccccg
     3181 gtttaccagt ttaaaccgtt catcgtgcag gggtgcggcg atctcgatat agtcgatcag
     3241 gggcatgtgt tcttccgcta tatttatccg gaattcggcg gcaagatatt ccgcaatcgg
     3301 actgtatcct tcatcatcaa ggaggatatt ttcgttccat ccgcgttcgc cgtcttcaga
     3361 cccgagttct gttacaagga agtaatatgc acgttcggcc tcgtatcgcg tcaggcgatc
     3421 aaagatctgt ttgtcctgga tgcaggacac aattatccgg gacaaaacaa agctggcaat
     3481 ttcatcatca tcacgctgct gaaaatgagc aatgaaggtt ttttttgaag ataaagcctg
     3541 gttgattcgc tttagtgcaa ggtcaatgat aatttttccc cgttcctgct tgagcagtac
     3601 atcaagggga ggaaatacat ctttgatatt gtcctgggca ttttttaaaa agggaaaatg
     3661 ggaaagttcc tttggtgatg gctgatagtc catcagtcag cctctatacg gggagccagc
     3721 aggtactcca cgtgaccgtt gccccccgct atgacgaatg agaacctgac agggtggtcg
     3781 attccaagga aaatctccac ttcatcagcc ttgctcatga ccttgcccat atcttttaag
     3841 taatcaaggg agaaaagtga ccgtgcttca accggtgtca gtgatttaag ctcatcttta
     3901 ctgaattcac gtctgatatt gtctgtatcc ccttcggcaa taaggaaaaa agtctgctcc
     3961 tttggattga ttccaagagc tatcttatcg gaaatcactg ccgctgcttt catcgcatta
     4021 ttaaaatcat caccggaaat tacaattttg cccgggagat tgatggtcgg ggggttggga
     4081 tccttacgga tcgtgttcgt atccagaagg gtgatggagt aatggtaccc gtgagctgaa
     4141 actgacatct tctgggcatt atcagcaagt tcaaggctga tgaggtcacc ttttccagcc
     4201 atgccaaaaa tatttttcat tttggaaata tctatgccca gctgactttt tgttgctttg
     4261 tatgaatcga aagcctcctt tttcagctcg ataccgatca tcgccacgtt agccgtgtcg
     4321 acagctcgcg tagagatccc attctcatct gtgtgaagcc ggcattcagg gacgagagca
     4381 gaaatcgcat caatgaattc cctgaacatg tctgcatcaa ttgttgcttt taacatcgct
     4441 tgtctcactt ctccttttga ccgtgtgtaa aaactaccct ttgtacctga aaccttttag
     4501 tgactttccc ttatacctac tttttacggg tctttttcca cggtgtaacc gttctacgtt
     4561 acgttattta ttactccata ataacttaac tgtagtcttt tggctttgat atccaacaga
     4621 tttcttcatg aaatttttgc tctaattttt gctttttttt gtttaataac gtaatgcaga
     4681 aaaagattga aaaatgatat aaattatggt tataccaggg aataatatcc caacccacca
     4741 caaaatatcg cacacaagaa aatgaccagt gaaatattga cggtttgttc ccctgaagtc
     4801 atttcacgcc attcattttt ctgattatgg gtaatgtatc ctgtaatgat tcctgtacct
     4861 gacccggcta atgaaccaag ggccacgacc atcagaatat caaaaatctc cgcactcatc
     4921 ctgtacctct tttcctgtta ccatcgcagt cctgttatca cgctgaggtt cttcaattct
     4981 ggtagggcaa attgtggtca taaggaatct ttcaaaagac ccctcttacc tcagtgaaac
     5041 ggttattttg tgatgctgat ttccatactc tctcccggtt tgagcacttt taccttcatg
     5101 tcagttgttc gttcaactgc ttctctgaac tttactggat ctgcagtgat cttgtcccat
     5161 gtattatagt gaatgggtat cacggttttt gccccgatga aatttgccgc aatcatagct
     5221 tctgaaatgc ccatcgtgta ccgtccgcca atcggaagaa gtgcgacatc gggatggtaa
     5281 agttctctga taaggttcat atcggaaaaa agggctgtat caccggcatg atagatcttc
     5341 aggccatcca taccgattac gtatcctgcg gcagagccgc cataatatcc cattccagcc
     5401 tcttctatcg aagaggagtg aactgcaggg gtcatcgtaa atgacacacc atcaacgatt
     5461 atagtacccc cgatattcat gccctcagct gcaatatccc ttgtcatgag gtacctggca
     5521 atctcggtaa ttgcgattgt cttcttaccg agtgcaacgg cttcccccat gtggtctgaa
     5581 tgaccatggg ttatggcaac gatatccggt ctggtataaa gaacgctgct tccttcgata
     5641 aagggatcga ttaggatctt ttttgatccg gcaagcagta cacaagcatg cccgagccag
     5701 gtgagttgca tatatgctga tctgaatatg gggggatgaa atttaccata tggatttacc
     5761 gtaaggctaa gtaaaaagcg aagtttcact aaaaaagaat gggcgtctga tgggatggac
     5821 ccttacatca catcaataag ttcagcttct gtgatatcaa tggaatcgcc aagactatcc
     5881 gcagtagcgc ttcgggtcat ctgctcggat atatcccggt cttccataac gtcccggacc
     5941 ctgtcaatgt agggatcaat tgtgggatct tcctgctcct cgatgacgtg ccggtattcg
     6001 cggagtgtgg atgggctgat accgagttcc tcagacactt ctttcatggt ctttccggat
     6061 tcgatcagtt gttccatctt cagcttatca aaaggcattt tgaaatcgag ttccctgaag
     6121 agttttaacc tcacccgtgc acgtgccacg gtcttgctaa gtttctcatc gccgagttgg
     6181 cgggcaattt ctgtatcatt ctttcccgca taaaacaagg tgacaagttt tgctagctgg
     6241 atcggtttta aggaggtttt gaaaattccc tgttccgtga tctctctcat tacgagagca
     6301 acctcacgtt cgatcgcgtc gctgtctctt aaagtaccat ggatattctt cataggttcg
     6361 actatctttg tcttgcctga catggtgctg aagagtttta gaagctgtac cttgcgtttg
     6421 tcttcggtca ttgcataccc cgtcatggga ttaggatata caatatttcg cctcctattt
     6481 aatcctatct taaagcagcg tttgaccaaa aaacctttaa attaatgcgg gattgcataa
     6541 ttaaaagctc ggttatgcag aagatcttgt atcccggttt gatattttta taaaaactta
     6601 tcggacgatg gtccgttgag acactcaata tccccattct cttttaattc acgcaaaatt
     6661 ttctttatat ggcagtaggg gcctttttca taaacagtca gactgattat tgcatggttg
     6721 ccatccagag gatcgcgttc ctctgtgtat tctttgatgg aatacgtcgg atcctttttc
     6781 agaatggtac ttaaaacacg ggattttgac tccctgtcat tttttatccg aagaacaaaa
     6841 taactcaagg atacgtctcc agattattat gttgtgagga taaccataat gctattcttt
     6901 tttttaaaaa tacattgtat ggtgatgcaa aatcctcaat tttcacggtc ctatgacgag
     6961 ggttataata tagtaaggga taattgattt agacaatccg gaataaaaaa gaagtggtaa
     7021 cgagtgattc agggatcttt ggatgttttt gagaaagtga tggacctcgc aaaacgccgt
     7081 ggttttatct ggcccacatc tgaatgctat ggtgcggtcg cgggctttat cgattatgga
     7141 cctcttgggg cgatgatgaa gaggcgtgtc gagaatatct ggcgcgaatt ttatgtcatc
     7201 cgtgaaggcc attacgagat tgaatgccca acgattgctc aggaagcagt ttttatagcc
     7261 tccgggcacg tcaagggttt ttctgataaa atgtgccagt gtccacactg taaagaatac
     7321 ctgcgtgctg atcatgttgc cgaggcggga aatattcata atgcctccac tatgaaaaat
     7381 gaagaccttg cagctgccat aacatcctac atttgccctg catgtaacga ggtgctggga
     7441 aacgttgcgg tcttcaattt caaccttatg ttttcgacaa ctattggtcc ggggtcgcag
     7501 aggataggct acctgcggcc cgagactgct cagggaatgt ttgttgattt ttcaagattg
     7561 ctcaggtttt accgggacaa gctgccgttt ggagctgtcc agattggaaa atcctaccgg
     7621 aacgagatct caccccgtca gggtatgatc cggctccgcg agttcaccca ggccgaagca
     7681 gaaatctttg tccatccgga tgagaagaac catcacccct ccttcaaacg gtatgcggat
     7741 tatagtatgc cgctgctcac gtatatccag cagcagaact gcgaggatcc tgttgaaata
     7801 actatgagag aagccgtaga ccgtgggatt attgcaaatg agtatgtcgc gtattatgtt
     7861 gccctgactc acgatcttct tgtaacaatt ggcataaaac cggaacgact tcgtttccgt
     7921 cagcaccttc ccgatgagcg ggcgcattat gctaccgact gctgggatgc agaggtattt
     7981 tccccgcgtt ttggctgggt tgaaacggta ggtatcgcag acaggacgga ttatgacctg
     8041 aaggctcaca gcggacagag cgggacgccc atgaccgtct tcatacagta tgaagaaccg
     8101 aaaaaaacag agcgtcgccg tattatcccg aatatgagcg tgctgggcaa gcaataccgt
     8161 gtgaaagcca aatctgtctt tgctgcactt gaacatgcga ctcccacacc tgatggtgcg
     8221 gatgtggaag tggatggcga gaaaatccat atccccgctg atctgtttga agtcagagat
     8281 gaagtgattg aggttcgcgg tgaagaaatt gtacctcatg tgatagaacc gtcgtacggg
     8341 atcgacagga tgtgttatgc agttctcgaa caggcttatg atgaagacat agctgatggt
     8401 gaatatcgga cagtaatgcg gctctcgccg aatattgcac cggtccaggt tgccgtgttt
     8461 cccctcatga accgtgatgg tcttgatacc ctggcaaaag atattatcac ttcattccat
     8521 gcacgcggta tccttgcaga atatgatgac agcggtgcta tcggacgccg ttaccggaga
     8581 caggacgaaa ttggcacacc gtttgctatt acggtcgatt atgaatccaa agagaacaat
     8641 acagtcacac tcaggaaccg tgacacgatg aagcaggtac gtatcgcaat tactgatttg
     8701 ccggggactg tcgatgctct tgtcaaagga aacatacctt ttgcatcgct tacaccataa
     8761 ccggatacta tgagtttcat cacccatcct ctcatcaaac cggaaagcat tgactcgcgg
     8821 gagtaccagc tctcaatagc aatgcgggcg ctggatgcaa ataccatggt aatcctcccg
     8881 actggtctgg gtaaaactgc ggttgcgctt ctcgttgctg catcaagggt ctataatgaa
     8941 gggggccggg tgctcatgct cgctccgaca aaacccctcg tggaacagca cctccgttat
     9001 ttcgaacggt atctcctttt aaagagcagg gaaggttcgg acatttcgcc ctttgtcatg
     9061 tttaccggtg aagccccacc cgatcaacgg acaagggaat ggaacaatgc gacggtaata
     9121 cttgcaacgc cccaggtagt aaaaaatgat ctgatcgcct gtcgttatac cttaaaggat
     9181 gtctctttac tcattgtgga cgaatgtcac agggccgtag gaaactatgc atatgttttt
     9241 cttgcacaac gatacctagc aactgccgac aaacccctcc ttctcgcaat gactgcatcc
     9301 cctggcggga ttttagaaaa ggtagaagag gtatcttcca atctgggtat cacgcatgtg
     9361 gagaacagga cagagcacga tcctgatgtc cggccgtatg tattcgaacg ggatattgat
     9421 tacatcagca tcgatctccc tccggaactc agagtttcga tcaagctcat caatacctgt
     9481 attgaagaac gtcttgcatt gctggcctct cttcacttta ccgtgccaaa gcgcgagaag
     9541 ctgacgatga aagcgttaaa cgagctcaat gcccagatcc agcagcgcat cgcaaaccgc
     9601 gatcctgtcg catactcggc agcgtcgtta tacgccgcct gcatgaagct ccggcatgcg
     9661 attacccttg ccgaatcaca gggaagcgaa gtattgaaag ggtacctggc aaaactggtt
     9721 gccgaaggaa cgggttccgg aggcagcaaa gcaagccagg gcctggtaaa agatcctgcc
     9781 ttccatgaac ttttcgaacg ctcccttcaa tggacagatg aactccatcc aaagcccggt
     9841 tttaccctca ctcttgtccg tgaccagttg gaatcccatc aggacagccg gatcataatt
     9901 tttgccacgt accgggatac cgtgcaactc ctggtggatc atcttacaaa aaatggtatt
     9961 gcatgtgagc gctttgttgg gcaggcaaca aaagatgccg aaaaagggct cacacaaaaa
    10021 aagcagattg ccatactgag caggttcagg gaaggcgagt tcagggtatt aatcgcaacc
    10081 tctgttggcg aggaaggcct ggatgttcca tcaaccgacc tggtgatctt ttatgaagct
    10141 gttccctccg aaatccgatc tatccagcgg aaaggcagaa caggaaggtc ccgtgccggc
    10201 agggttatag ttctggtcac caaaggaaca tcagacgaag tgtaccgcca tgtaagtcag
    10261 gcaaaagaga tgatgatgca caaaagcatg cgggaaattg tcaggacaaa tgccctgcag
    10321 cagcaacctg ttttgccaca acaggcaagt attgacgagt tcacggttca gggaccaggc
    10381 ataatcattg acgatcgcga gacctcctca aaggttgtgg aagtactctc ggggctgggg
    10441 gctgatatcc gcctcaaacg acttccccat ggcgattatg cgattggtga ccggatcctt
    10501 atcgagcgga aaacagcccg ggatttcgtt gataccctga ttaaccggga tttacttggg
    10561 caggtcaagg caatggcgga agttgtacca cggccggtga tgattattga gggaggagat
    10621 ctgtacaccc agcgtgatat tcatccgaac gctatcaagg gagtgctggc agcacttacc
    10681 attgacatgg gggtctcact catgtttacc cgggatgaac aggataccgc acagatgctc
    10741 ttcatccttg caaaacggga ggaaagtgag cggggggaac gcaaggtcca cccgcacaaa
    10801 tcgcaccgct ctgtccgcga ggaccaggaa tatatcgttt ctgcgtttcc tgaggtaggc
    10861 atgaaaaatg cacgtcttct cctctcgcac ttcggttcaa tacgggcaat aaccgatgcc
    10921 ggactgccgg aattaactgc aataaaagga attggtgaga agactgcaca gaagatcttt
    10981 gatctctgcc gccattctta caactgacac aggatctgaa gtgcctcagt acccgctttc
    11041 actgcattca tcagtgcaag cacgttttcc gggtcttttt cattttgaat gcgctcacag
    11101 agagaaacga tggtcatagc gagttcatct gtcagtaatc cgccttcgca tgtctcttca
    11161 tggtgctccc cgcaactgca ttcctggtca tgttcctgat gtaccggcgc agccgttgaa
    11221 gggccgggtt ttttaactac ctcattctct gcgcagacaa cacacagggt cttgcccttg
    11281 acttcaaaaa ggggacatcc gcaggtttta caggactttt cgagcatttt tcccccttta
    11341 aggaggtatt ctgccataat ttcatcttct ttacgtaccg gcattcaatc accgtcgact
    11401 caatatttaa cttatatatg tcggttctac gtattaacta caggtacaca tgaaggtgat
    11461 accatgccca accctgaaaa aactatggaa aactgtattc tgatgctgca gcgcattcag
    11521 gaagacagct caataccccg aaacatccgg cgcgtagctg atgagacccg gacattgctg
    11581 acaaacaata ccaaagcaat gggactgcgc gctgcagagg caatttctaa gattgatgaa
    11641 atctccaatg atcccaatat gccgattcac gcccggacaa ggatctggga gctcgtatcg
    11701 cagctggaga ccattcccct tgactgatac gaaaggatat tacttttacc aggtacgaga
    11761 gcaggatttt tacctataaa aatctctcct ggagcatggc gatttatgta tgacggtata
    11821 ccccttttgg tatatgcatt tcaaaaaggg caccgtgttg gaattccccg gtttctttta
    11881 tcgtaatatc cgtaatggaa aggatttccc ggcaaaggaa taacccgagc ccggtatttt
    11941 taccaaaccc tctgacaaaa atcgtttcct tgtcatggga gggaatgccg atcccgtcat
    12001 cttcaatccg gatcaacaga ccgtcgggta attcaaccga tgacattttg acgacagaca
    12061 cgtgatcccc atgttctacg gcattctgca aaatattata aaatacccgg tccagcaggg
    12121 gatcggcaaa tatttcaagt ccgtcaactg atgattcgaa ccgtattttt ttaatatcga
    12181 tgtgaacaca ggccctgtag aaggtgtcgc tcacgttctt ccagttcgga gcaacgaccc
    12241 caagatcctg atagagcttg gtaaactcaa tattttccgt aatcatccta actgctgctt
    12301 tgagtttttc cagatacatg gaatgctggg gttcaggtgg atggtcgtta aacagttcaa
    12361 gataccctcc aagtacggtg agcttattcc ggatatcatg ccgggtaatc tctgcaagaa
    12421 ggttgagttt cttattggca agctgcaatg catcctgtgt ttttctgata tccgaaatat
    12481 cgtggaaaat ccccatcatc gactggcgtc cgcccaggtc gagtattgtt gcgctgacga
    12541 ttaccgggat acgggcgcct ttatggttaa ccacttctgc ttgtaagcct gtgccctcct
    12601 gagcatgttt tttgaaatat tcatgatatt tttctttatt ctcgacaggg aaaagctcac
    12661 tttcatgcat ggtatggatt tctgcgagtg ttttgcctat catcttttgt gctttaatat
    12721 tggcatcgag aagaacaccg gtttcctgat cagagataaa aatcgcatca tttgcatggt
    12781 ccatgagacc cttggtcttc tcaaatgcag acttgagttc atcttcagcc cgtttacggt
    12841 cggtaatatc acgcacgaat gcaaaaatat atgccagaga cccaaattcg atatagttaa
    12901 aaagtatctc caccggaatg acagagccgt cttttttcct catggaatcc tggacggtga
    12961 tatatctttt ttctttcatc ccggcccaga ggcttcccca gtgattttcc atgaattcag
    13021 cgttgatatt cctgatcgaa agactggtga attcctcccg taaatatccc agcattgagc
    13081 agatggtgtc attgaaaaac gtaatccccc cgttttcatt tatccataat atgccttcgg
    13141 cagcattact cacggtggcc ttcatcatgc ggagctcttc ttctgctttt tttctccgga
    13201 atgcctgccg taccttatgg ctgagttcac tgaactgggc acggggatct ccgcctttct
    13261 ggaggtaaaa gtccgcccca ttatttatgg cctggacagc aacctcctcg cgtcctttgc
    13321 cggtgaacag gatgaacggg atatcatcaa atttcatcct tacctgtttg aggaattcaa
    13381 taccgtccat gaacggcatc tgatagtctg aaacaaccgc atcaatcatt ccttctgaaa
    13441 ggatttgcat cccctccttt gcagaagtga ccgtaacgac agagaattct ccctcctttt
    13501 caagaaaaat cttacagaga tcgagcagtt ccggctcgtc atcaacgtaa aggagagaaa
    13561 tcattgattt ttatattgtc cagtcaagga ttaagttttt tgtgaaaaga atttataaaa
    13621 accaggaaga agccggcgtg catgaacacc ggtattgata tccgggtgat tgggccagga
    13681 ggaaactggt atgtcccggt caccggggga aggtttttta ttaaaatgaa aaaaggatca
    13741 gatataccgt tttcatacac aatccatgtt tttacgatca tcgtgatgac ccaaaatgaa
    13801 acgcatcctt cgggacaatg atttcaaacc gggcaccttt tctgaattcg cccgtctccc
    13861 ggatcgataa accggtgctt gaaagaatct tctgtgccag atgaagcccg gcattcttat
    13921 cattgcccgg tctccattca aagagctggt ctttagtttc cggcactatt ccgattccgt
    13981 tatcggaaac aacaataatg agttcttcag gagtgaaaga ataagatata tcaatacgcg
    14041 taactttttc cccgaatcta tgtgcgaagt caaaaagaag ataaaagacc ttttcaaaaa
    14101 gggggtctgc aaagacaaca atctcatcct gctgcacgtt taaggtaatt gaatctgttt
    14161 caatatccaa aaaggcgtga tgaataactg tcggtacaat ctgccatgtg ggaatttctg
    14221 caccgagatc ttcgaattcc cgggtaaaaa gaatctgcct ttgtatggtt tttgtagcgg
    14281 tctgggcgcg attaagatgc ttttgtaaat caggatcctg tacactgtac gatgcaagtt
    14341 caagagaacc actgacaatc atcagctgat tcaggatatc atggcgtatc cggcttgaaa
    14401 aaacatccag gtttttccgt gcaatcttct gtgcggtctc atttcgtttt ctttttgaga
    14461 tagaatacac ggaacactgg atgatctgcc tgtcatcaaa tgaggtaatt ctgctgaaaa
    14521 attccacatc gaccgatgtg ccgtcttttg ttaccagcgg aatatcgcca tagtgcacat
    14581 ggtcggattt tttcaattcg gtaaagaaca gatcagctcc tgaagcatcc ttaaaaaatc
    14641 ctgcatcctc aattttttta ccaataaggt attcgcggga gtaaccggta agtgaattaa
    14701 tccacgaatt tgcttctata atcttttttg tgtcaggatc aagaatcagg agaccatact
    14761 ctgctgtctg gaataaatga tggaactttt catccttccg atgcatgttt cctatttcag
    14821 taagcgtttc aatggcaccg gacagctggc cgttttgatc atataacagg gatgcaactg
    14881 cttcaagagt ggctggcttt cctctcagat cagggacgtt tatttgtgcc cttacacagc
    14941 cccgggtcct ttgtaaagcg gaatagtggt agttttcaat ttcggtgtct gaagcaaaaa
    15001 ccaggtcaag gagcccggga atgcgttcac ccagaatcct gaatgagtac tcataatcgc
    15061 cgcgggcggt tacctgttca gcactgacag cggtcagttt ctccatcgcc ttgttccaga
    15121 taattataaa acccgctgta tctgtagcaa atgtcgcaag gggaaggaaa ttgattattt
    15181 gagtcagtct gtgctccgag agttccaggt ctccctggac ctgcctgctg ctgtccagca
    15241 tatccattgt tatgaacacc caggagatct cctgagaaat gtcattgagc aacctgatct
    15301 cagtatcggt aaaaaagtta ttctcgacag aatagagggt gatcgcccca cgggtggctt
    15361 taccactcct taaaggaaaa gcagcagcgg atcggtaccc cttgcggata gcatcgtctt
    15421 cccacatctg tagatcagga aaagcctgga tatcattgca gattgtatat tttccttttt
    15481 ttatggtagt gactgtcggt ccatgtccct gggaaatatc ttctgaaaat aatctgactt
    15541 taccaaagaa ttcgtctgtt tccccactcg caaagacggt gtcaatcctg gctgtgacag
    15601 gatcttcaaa tcctatccat gccaggacaa atcccccctc ctggatcatg atcctgcaca
    15661 cttcctgagc cagttgccgg cggtcatgga tatggacaac tgcctcgttt atccggcggt
    15721 gcagggcatc tatccggtta agcagtgcaa tcttttgttc atttccccgg aattcgacag
    15781 cgtttctgag cacatgggat aactcggcaa actgggaagt ttgatctccg ccttttttga
    15841 cataatggtc aaccccgttt ttaaaggctt cagcaaggaa ttcctccctt tttttatcgg
    15901 taaaaaaaat aaaggggagt tttggaaact tttcgcgtac gctcctgagt acctggatac
    15961 cgtcatcggg cttgtcataa tctgcaatca ctgcatcata tgatgcggtt ttgagtatgt
    16021 tcagggcatc cactgctgac tgggtggttt gaacccgtat atctgcatca tgttccaaaa
    16081 aacgcttacc cgcctccagg aaggctgtgt cgttaccaat ataaagaacc gatatcactg
    16141 caactacctt cagcagacca ataattcatt ctatttacat tcggcaagaa agttataaat
    16201 gtaccgttca ttgtgcagtt taacatgcat tctttacctt ttctatattc cctgacgata
    16261 atcctttatt tcaccggcat cttccgcata tattttttta caaacaatgc ttataggtag
    16321 tatactcatg gattttattt ccattatcct tatcattgcc ggcctggtct tattcgagac
    16381 catcacgagc atcgataa
//
LOCUS       DASRLV010000161         4963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_151839,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000161 DASRLV010000000
VERSION     DASRLV010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151839"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..286)
                     /locus_tag="VFC43_06320"
     CDS             complement(146..286)
                     /locus_tag="VFC43_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30831.1"
                     /translation="MLDIFKKDRTAYVHYNYAIIAARQGIVNVPHTQAAADKRAGPER
                     SI"
     gene            483..553
                     /locus_tag="VFC43_06325"
     tRNA            483..553
                     /locus_tag="VFC43_06325"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:515..517,aa:Gly,seq:ccc)
     gene            562..1245
                     /locus_tag="VFC43_06330"
     CDS             562..1245
                     /locus_tag="VFC43_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5612 domain-containing protein"
                     /protein_id="HZK30832.1"
                     /translation="MEMEPERCAIRIIAENRKGVLRDIATIVARHEANIVMINQEVFD
                     SGPYTGMAELYFEYDAGEGSDRDMLIADLNDIPSITNVRAYQPFGHIFGSRVIIIGGG
                     AQVAQVAMGAVNEADRHNIRGERISVDTIPLVGERTLALAVDAVARLPRASILVLAGS
                     IMGGEISKAVDRVRDAGIPVIALKMAGTVPDHADLVVTDPIQAGVFAVMHISSKAVFD
                     INRVRGREF"
     gene            1247..1825
                     /locus_tag="VFC43_06335"
     CDS             1247..1825
                     /locus_tag="VFC43_06335"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZK30833.1"
                     /translation="MDIVEKAVSVLMHDGTIIYPTETVYGLGADALSDDAILEVYEAK
                     KRLLGMPVSIAVADFDMLAAVARVEPGMHGFIETFLPGPVTLILPSRRIIPEILTGGT
                     GLIGIRMPAHDLALRIIERFDSPITATSANLHGAKDPQTPEECTVPRELFIDGGRLPG
                     IPSTVIDLTNKKIIRRGAQAEAVEAFLGTLIP"
     gene            1822..2745
                     /locus_tag="VFC43_06340"
     CDS             1822..2745
                     /locus_tag="VFC43_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HZK30834.1"
                     /translation="MMPVRLRDFIEDPDGWLYAVSTYDNDDRVGCVLRYVPDVNGERT
                     NHAGKRYKKFDFEDAFAFIEHHKPHYSDLVQRVLVQDIKNVLKPDREISRIAACHPRV
                     QKLLDLFRLPKGSVGCTGSLLCGLENDQSDIDLVIYGKDWFIAQRMVRQGILDGQVEG
                     LSADMWQKVYEKRKPEIPYDQFVLHELRKWNRGQIDGTYFDILYTRSYHELKNAPAGK
                     GTVIGRMVIEAKVTDASLAFDSPAVYEVEHESVSRVLSFTHTYSGQALTGETIEACGI
                     CEQHGTEQWLVVGTTREARGEYIISRTLLGR"
     gene            complement(2717..3127)
                     /locus_tag="VFC43_06345"
     CDS             complement(2717..3127)
                     /locus_tag="VFC43_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30835.1"
                     /translation="MTSKRIESGTPEGHAMGFSENLFTGWLERQDDNRLYLHYIISRS
                     RNEGNTQALLRQWLLDGYDVRVVMPRPIMQHILKKYYFAPSREYFPDYYEDEVEVWIR
                     SGTLQPGGARVPQAEKSGPGCSLLPFTGQVKSLI"
     gene            3275..3673
                     /locus_tag="VFC43_06350"
     CDS             3275..3673
                     /locus_tag="VFC43_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HZK30836.1"
                     /translation="MAVKNPFRTTLIAPCGMDCAICMAYLREKNHCGGCYAPDRKCHK
                     NCSIFGCDQVRGRYHHDCAEFPCKSLRQLDMRYRKNYGMSMIENLEAIGQHGIRKFIK
                     SEKERWTCSKCGGVICVHRGFCSTCGEKKE"
     gene            complement(3693..4454)
                     /locus_tag="VFC43_06355"
     CDS             complement(3693..4454)
                     /locus_tag="VFC43_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HZK30837.1"
                     /translation="MDMPFPKSSGERFNKYNPSCFLTVIRGIILAAQTDPVTISELVR
                     VHSCPVRFYYERNIPVVESDRYTVCKQLSYHLGNPLVSEVIWNEVVSVNPGIDPEMRS
                     FLETCIASCNKNTWKEADQNDVRVTSDRHSITGMVDRMYGDGTFCIIRAAGAMPFGTY
                     AADRLRIACIALCLEEMTGTEVIGGNVEYIPDGVSRFHTVQPRDRRQVIATLHKIRQI
                     RNGDIPHPPLNAPCSRCGYKDRCENISGRKLSDLL"
     gene            4505..4801
                     /locus_tag="VFC43_06360"
     CDS             4505..4801
                     /locus_tag="VFC43_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HZK30838.1"
                     /translation="MTGQEISVNIPPTLDPVYSNMIQIAYKDDEFTFMFLHQLPQVNQ
                     ARAKAIVSITPTHAKNLLGVLTKTVADYEAKFGTISPPKEPQGTEKVTTLRGYS"
     gene            4847..4918
                     /locus_tag="VFC43_06365"
     tRNA            4847..4918
                     /locus_tag="VFC43_06365"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4879..4881,aa:Thr,seq:cgt)
BASE COUNT         1275 a         1207 c         1282 g         1199 t
ORIGIN      
        1 ggtaaatgag gtctggtaaa tcacggtcgc cggagtatca tctgaaactg cctgtatggg
       61 tggtattacc accagtaggc agacagcaat aatgacggat atcaggtatt tttttatctt
      121 cataagggtc tctccaattt aattctcaaa tagagcgttc aggcccggct cgtttatcag
      181 ccgccgcttg tgtgtgcgga acattcacaa tcccttgccg ggctgcgatg atggcataat
      241 tatagtgtac atatgcagtc cggtctttct taaaaatatc gagcatgcag ggaaaacaac
      301 cggcaacata ccgggagcat ggtaaccgga cgcaggatca gtcattaatt ttttaaagaa
      361 taggtttttt gtccctgtag gcgtgtaatt atactttttt ttatcagccg gccaagaatc
      421 gatcacataa cattatattt atctttgaaa tcacgcaata ttagagaaca aagaggaatt
      481 ctgcgataat agtctagcgg taggacagga gcttcccaag cttctaaccc gggttcgatc
      541 cccggttatc gcatcggttt tatggaaatg gaacccgaac gctgcgcaat acggatcatc
      601 gcggagaacc gcaaaggtgt cctgcgtgac attgcaacta tagttgccag gcatgaagca
      661 aatatcgtaa tgatcaacca ggaggtgttc gattccggac cctataccgg catggccgaa
      721 ctctattttg aatatgatgc cggcgagggg agtgaccggg atatgcttat cgcagatttg
      781 aacgatatcc cgtcgattac gaatgtgcga gcctaccagc cgtttggcca tatcttcggg
      841 tcacgggtga tcattattgg tggcggggca caggttgcac aggttgccat gggtgcagtg
      901 aatgaggctg accggcataa tatccggggt gaacgcattt cagtagacac cattccgctc
      961 gtaggtgaac ggacactcgc tcttgccgtg gatgcggtag cccggcttcc ccgtgcatca
     1021 attctcgtcc tcgccggatc aattatgggg ggggagattt caaaggctgt tgatcgtgtc
     1081 cgggatgccg ggataccggt cattgcactg aaaatggcag gcacggtacc tgaccatgcc
     1141 gacctggtgg taaccgatcc catccaggcg ggagtgttcg cggttatgca tatcagcagc
     1201 aaggcagtat ttgacatcaa ccgtgtccgt gggcgtgaat tctaagatgg atattgttga
     1261 aaaggctgtt tcagtgctca tgcatgacgg gaccataatc tacccgactg agacggtcta
     1321 cggactcggt gccgatgcgc tgtcagatga tgctattctg gaagtctatg aggcaaaaaa
     1381 acgtctcctg ggaatgccgg tatcgattgc ggtagccgac tttgatatgc ttgctgctgt
     1441 tgcccgtgtc gagcccggca tgcatgggtt cattgagaca tttcttcccg ggccggttac
     1501 cctcatcctc ccttcacgca ggataattcc ggaaatcctt accggaggaa cgggattgat
     1561 tgggatcagg atgccggcac atgacctcgc attgcggatt atcgaacggt tcgattctcc
     1621 tattacggca acaagcgcca atcttcatgg tgcaaaagac ccgcagacac ccgaggaatg
     1681 caccgtcccc cgcgaacttt ttattgatgg tggcagattg cccgggattc ccagtacggt
     1741 aatcgacctt acgaataaaa agatcataag gagaggagcc caggctgaag cagtggaagc
     1801 atttcttggt acactaatac catgatgcct gtccgccttc gtgatttcat tgaggatccc
     1861 gatggctggc tctatgcagt ttccacgtat gacaatgacg atagggtcgg ctgtgttctc
     1921 aggtacgttc ccgacgtgaa tggcgaacgc acaaaccatg cggggaaacg gtataagaaa
     1981 tttgattttg aagatgcgtt tgcgttcatc gaacaccaca aaccgcacta ttcagacctc
     2041 gtccagcgtg ttctggtcca ggatataaaa aacgtgttga aaccagacag ggagatatcc
     2101 cgcatcgctg cctgccaccc ccgggtgcag aaactcctgg atcttttccg acttccaaaa
     2161 ggatctgtcg gatgtaccgg atcacttctc tgcgggcttg aaaatgacca gtccgacatc
     2221 gacctggtca tatacggtaa agactggttt atcgcacagc gaatggtcag gcagggaatt
     2281 cttgatgggc aagtggaagg gctttccgca gacatgtggc agaaagtgta cgagaagcgt
     2341 aaacctgaga ttccgtatga ccagtttgtt cttcacgaac tacgaaaatg gaaccgcgga
     2401 cagatagatg gtacttattt cgatattctc tatacacggt catatcacga gctgaaaaac
     2461 gcaccagcag gaaaaggaac cgtaatcggc aggatggtga tagaggcgaa agtaactgat
     2521 gcatctcttg cttttgacag cccggcagta tatgaagtgg agcacgaatc agtaagcagg
     2581 gtgctctcgt tcacccacac ctacagcggc caggcgctta ccggagagac tattgaagca
     2641 tgcggaatct gcgagcagca tggcactgag caatggctgg tcgtggggac aacccgggaa
     2701 gcccgcgggg agtatatcat atcaaggact ttacttggcc ggtaaatggc aaaagagaac
     2761 acccgggacc tgacttttct gcctgcggga cccgtgcacc gccgggttgc agggttcctg
     2821 accgtatcca gacttcaact tcatcctcgt aatagtcggg aaaatattcc cgggacggtg
     2881 cgaaataata ctttttgagg atgtgttgca ttatgggccg tggcatgacc acccggacat
     2941 cgtagccatc caatagccat tgcctgagga gcgcctgggt attgccttca ttccggctcc
     3001 gggatatgat ataatggagg tagagacggt tatcatcctg cctttccagc cacccggtaa
     3061 agagattctc agaaaatccc atagcatgac cctcaggtgt tccactttcg attcgctttg
     3121 acgtcatttc atcacaatcc gcagggatcc agagggttga gaggagaagg ataaaagaaa
     3181 aaggcatggg catggtgaaa gtaattcggg ctgtggtaca atggttgcaa caacatgtcc
     3241 caagtttttt attggccgca gcctatcaga ttctatggcg gtgaaaaatc cgttcaggac
     3301 cacgctcatt gcaccatgcg gcatggactg cgccatctgt atggcgtatt taagggaaaa
     3361 gaaccattgc ggcgggtgtt atgcacctga ccggaagtgc cataaaaact gcagcatatt
     3421 tggctgtgac caggtaaggg ggaggtatca tcacgattgt gcggagttcc cgtgtaaaag
     3481 tctccggcaa ctcgatatgc gataccgcaa aaattatggc atgagtatga ttgagaacct
     3541 tgaggcgatc ggtcagcacg gcatccgtaa atttataaaa tccgaaaaag agcgctggac
     3601 ctgttcaaaa tgcggggggg taatctgtgt tcaccggggt ttttgttcga cgtgtggtga
     3661 aaaaaaagaa taatctgttt ttttttatga tttcataaca ggtctgacaa cttccttcca
     3721 ctgatatttt cgcaccgatc cttgtacccg cacctggaac agggagcatt gagcggcgga
     3781 tgggggatgt ccccgttcct gatctggcgg attttgtgca gtgtggcaat cacctgccgc
     3841 cggtccctgg gctggaccgt atggaatcgt gaaaccccgt ccgggatata ttccacgttc
     3901 cccccgataa cctctgttcc ggtcatctct tcaaggcata atgcaatgca cgcgatccgc
     3961 aacctatctg ctgcataggt tccaaaaggc atggcaccgg cggcacgaat aatacagaag
     4021 gttccatcac catacatacg gtccaccatg ccggtgatac tgtgcctgtc tgacgttacc
     4081 cgtacatcgt tctggtctgc ctctttccag gtgttcttat tgcaggatgc tatacaggtt
     4141 tcaagaaacg atcgcatttc aggatcgatc cccggattaa ccgagaccac ttcgttccag
     4201 ataacctcag aaacaagagg attaccgaga tgataggaaa gctgtttaca taccgtatac
     4261 ctgtccgatt caacaacagg gatatttcgt tcgtaataaa acctgaccgg gcaggaatga
     4321 acccgcacca gttccgaaat tgtgaccggg tctgtctgcg ccgccaggat aattcctcgt
     4381 ataaccgtta ggaaacagga aggattatac ttattgaacc gctcgccgct gctcttggga
     4441 aacggcatat ccatgaaaaa aactgaccaa cgtatttcat accagtacaa ccaatctgat
     4501 cagcatgaca ggacaggaaa tttccgtaaa tatacctcct acccttgacc cggtatacag
     4561 caacatgata cagatcgcct acaaggatga cgagttcacg ttcatgttcc tgcaccagct
     4621 cccccaggtg aaccaggcac gggccaaggc cattgtatcg atcacgccaa cccatgcaaa
     4681 aaacctgctc ggcgttctga caaagaccgt ggctgactac gaggccaagt ttggcacgat
     4741 atcgccccct aaggagccac aaggcaccga gaaggttacg accctgagag ggtattcctg
     4801 atacgcatat ttttttacca taagcaccga tattgtgagg tattgcgccg ccgtggctta
     4861 gcggcatagc ggctgattcg taatcagcag gtcgagggtt caattccctc cggcggctta
     4921 cagatagaag gttacaatcc ccccaaccct tgagatctgc caa
//
LOCUS       DASRLV010000162         8193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_219825,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000162 DASRLV010000000
VERSION     DASRLV010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 8193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8193
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219825"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VFC43_06370"
     CDS             complement(<1..661)
                     /locus_tag="VFC43_06370"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HZK30839.1"
                     /translation="MHENSSIVDALSHSLVAWILFSAAGFVPLLPFAVLGTVIPDADI
                     FYSFISDKAPSLYLFTHGGISHSLAGAFFLSVLSYGVVTSIALIGIIPFSLVAIGGVS
                     GFAAVLTGSLLHLAIDVLACPGIPLLAPVSDRKYTIGILPGPSILLACAAVGLVLVTV
                     TRLMEFSFALHFYTLIVLIYLAVRGVFFLYAGIRIQGRKVPKVKPFSWLAISEEEDHC
                     TV"
     gene            761..1201
                     /locus_tag="VFC43_06375"
     CDS             761..1201
                     /locus_tag="VFC43_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZK30840.1"
                     /translation="MTTETRELNPDEFYLAEKLWEQYRGQKADHPKERIFGVFVDGVL
                     AATARYTRHPDGVEMDCVFVSESFRGKGYARFAVEELIRQCGSEPIYIHSTIVLISFY
                     KTFGFTPIPEDQLPLSIKERFLFCFGEMMGCNVCPMLRKGSAVQ"
     gene            complement(1697..3103)
                     /locus_tag="VFC43_06380"
     CDS             complement(1697..3103)
                     /locus_tag="VFC43_06380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZK30841.1"
                     /translation="MTGESILVVEDEAIIALQLSELLKKNGYQVAGMTANGEDAVMMA
                     EKNSPDLIFMDIELMGKMDGIEAACTIHERANIPIIYLTAFSDIQRIARAKETMPYNY
                     IVKPFNEQELLASVDMALYRHTVEQRLRESMQRYRTIVDNAAEGIMIISCETRTILET
                     NPASIRLLGYTAGELTGMTVDNLIAAPDGKAWGERACSIGGWSGEVQFRHRDGSLRDV
                     ELTSSIIHQKGSPTLSCFVAHDVTDRKRAEVALRQALRKLNILSSITRHDILNQLITL
                     RGFLSILEQKQPNPTLREYFQKINAAAQRISAMIRFTKEYEEIGVHAPSWLDTRTIIS
                     TAVKETSLGKVKLNYNLPSGAEVYADPLIVKVFYNLMDNALRYGQKITTIQFSMQESG
                     DGHLIVCDDDGVGIPAEEKEKIFDRGFGKNTGLGLALSREILSITGITIRETGMFGSG
                     ARFEMLVPAGSFRYHKPG"
     gene            complement(3383..3568)
                     /locus_tag="VFC43_06385"
     CDS             complement(3383..3568)
                     /locus_tag="VFC43_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30842.1"
                     /translation="MNAKTTNYVVIIAGLILLIVGVGIQPGNYSILNYVAIIAGIVII
                     ILGIWSLKSLSWFAKHT"
     gene            complement(3651..3803)
                     /locus_tag="VFC43_06390"
     CDS             complement(3651..3803)
                     /locus_tag="VFC43_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30843.1"
                     /translation="MTKITKLARILAVIIGVIVIVLSFAKFFPLSNEGLVLAAFLTCW
                     GLDALI"
     gene            4150..4395
                     /locus_tag="VFC43_06395"
     CDS             4150..4395
                     /locus_tag="VFC43_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30844.1"
                     /translation="MPSKDDIRVKAHKAVDNARVAVNEVVDDAKTAVHNATTRKGTVV
                     HHPTAQKTVDDKKIEAHKVSSDAKIRTHRKAEEANKK"
     gene            4446..4685
                     /locus_tag="VFC43_06400"
     CDS             4446..4685
                     /locus_tag="VFC43_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30845.1"
                     /translation="MWALTKEIKETKAAGEARTSNGVSNNDLSSDLINSPALEILWQK
                     KGKIFCNCISSRNGECIQIRKGGKYLVRIQLVAFS"
     gene            5117..5527
                     /locus_tag="VFC43_06405"
     CDS             5117..5527
                     /locus_tag="VFC43_06405"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZK30846.1"
                     /translation="MTSPDGPPDPFFARDAWAQEANIELMEVSAGRAKVRMRIAGWHM
                     NSHGTVHGGAIFTLADTAFALASNSHGVPAAAINAHISYVKAATAGTLYADAEEFSLN
                     PKIATYMVTVTDDAGEKIAIFEGMVYRKTPGRDK"
     gene            complement(5602..6216)
                     /locus_tag="VFC43_06410"
     CDS             complement(5602..6216)
                     /locus_tag="VFC43_06410"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HZK30847.1"
                     /translation="MGREGIVDIERQAELGGPTHTKGVLILGGYLADRYARDKPLSLA
                     AKLIFEQSYGGVDGDSASSTELYAILSALSGLPIRQSIAVTGSVNQRGEVQAIGGVNE
                     KLEGFFEVCKARGLTGKQGAMIPASNVQNLMLKEEILEAAKAGKFTVYPVKTIDEGIE
                     FLTGVPAGEPLADGSYKEGTVNYLVNKRLQQMAETVRDFQVPAR"
     gene            complement(6231..7934)
                     /locus_tag="VFC43_06415"
     CDS             complement(6231..7934)
                     /locus_tag="VFC43_06415"
                     /inference="COORDINATES: protein motif:HMM:NF025038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK30848.1"
                     /translation="MRPTEEIIGQERAQRALRFGLEIQERGFNIYVAGMPGTGRRTAV
                     RNFLDELAKTKPKADDWVYVNNFTNPYEPLAIRLPPGMSSQLKKDMAFFIKEARRALP
                     RAFDSDDYAKKRSEALGKLNKERDDVVGQVNESAAKQGFTIQIGPTGLIIIPVVGGKP
                     LTQEEFEALPKETQAGILQRREALDIDLRSGFRQVRELGAKEMEVVTQLNSEIALYAI
                     GHLLAGLKEKYGKISDVLPYIDSVQKDILDNLSIFLGSAQQQQQQQQEQVPPPFQQWL
                     AKDLAFRKYEINVVVDNGSIAGAPVVFEETPSYPNLLGRAEREVQFGVVTTDFMMIRP
                     GSVHKANGGYLVIPVLDLFRYPLAWDGLKSALKTEKIRIEEPGEQAGFITTRGLKPQP
                     IPLSVKVILIGTPDINQILHQGDPDYPELFKVRADFDTVMDRNEENTKKYAAFICTLC
                     KRFSLKHLDNTAVAKVVEYGSRVADDKKKLSTRFSGVADLIREANFYAIQEKAPYITV
                     KHILKAVEEKVYRSNLIQQKIQVFIARGVFLIDTEGSKTGQINGLSVMEMGDVAFGHP
                     A"
BASE COUNT         2013 a         2086 c         1946 g         2148 t
ORIGIN      
        1 gtacggtgca gtgatcctct tcctcgctga ttgccagcca ggaaaaaggt tttaccttcg
       61 gcacctttcg tccctgaatc cgtatacccg catacaggaa aaataccccg cggactgcaa
      121 ggtaaatgag gacgatcagg gtgtagaaat gaagtgcaaa ggaaaattcc atcagccgtg
      181 tgaccgtgac caggacaagc ccgactgcgg cacaagcgag caggatgctt ggacccggca
      241 ggataccgat agtatatttc ctgtcagaaa ccggtgcaag cagcgggatg cccgggcatg
      301 ccaggacgtc aattgcgagg tgaaggagcg atccggtgag gactgccgca aaaccagata
      361 ctccgcctat agccacgagt gaaaagggaa taatcccaat gagtgcgata ctggtgacaa
      421 ctccatagga caggacagat aaaaaaaaag cacctgccag actgtgagaa atacccccgt
      481 gagtaaacag gtagagtgac ggggccttat cggaaataaa agaatagaaa atatctgcat
      541 caggtatcac ggtcccgaga accgcaaacg gcaggagtgg tacaaagccg gccgcggaaa
      601 acaggatcca cgcaacgaga gagtgggaga gagcgtccac aatggatgaa ttttcgtgca
      661 tcacaaaaaa ggctgctggt cagggaacta ataaacatac tccagaaagg cacgctcaaa
      721 tagttatttt acctaacagg gtatgtggca cagaactatc atgacgactg agacccgtga
      781 actgaaccct gatgagtttt acctggcaga gaagttgtgg gaacaatacc gcggccagaa
      841 ggcagaccac cccaaagaac ggatattcgg ggtctttgtc gacggagttc tcgccgcaac
      901 agcacgctat acccggcacc ctgacggcgt ggagatggat tgtgtctttg tctccgaatc
      961 ctttcgcggg aaggggtatg cccggtttgc cgtagaagag ctgatcaggc agtgcggttc
     1021 agaaccgatc tacatccatt caaccattgt cctgatctca ttttacaaga cgtttggttt
     1081 tactcctatc cctgaagacc agctccccct tagtatcaag gaacggttcc tgttctgctt
     1141 tggagaaatg atgggatgca atgtctgtcc catgctcagg aaggggagcg ctgtccaata
     1201 agtaaagata ccggttgagt ctccctggca gagatgaaag agtaataatt ctctttacaa
     1261 tctcttccag cgtcttttta tttctgctct ggagaaacgc acatgaaccg attacgttca
     1321 cacccaaatt ctgaaaaatc agttttactc cacgatgaac ccccgcctca ggacctctcc
     1381 gaggcacggc gggcaggatc tgttagtcat taatccgccg cgggggtgtc cctttttggt
     1441 gcggcggaat tcatcataac tgaccgatag aagggttcca attccaaata gcatctgata
     1501 accatgattt tcatgggaaa tcatttttga aaattctaac gctcttttgg gctttgcccc
     1561 caccactgga tcatccccat tgcgatgaca atgtattacc tgctatctaa ctcggtttat
     1621 cacccttgcg ccccgtcccc cgacgggggc cttatcggcg caaagaggag acattatttt
     1681 accagaaaaa ttcaggtcat cctggtttgt gataccgaaa cgacccggcc ggcaccagca
     1741 tctcgaaccg ggcaccgctg ccaaacatgc ctgtttcccg gatggtgatg cctgtaatcg
     1801 agaggatctc acgggaaagg gctaatccaa gcccagtgtt tttcccaaac ccacggtcga
     1861 agatcttttc tttttcttca gccggaatac ctacaccatc gtcatcacaa acgattaggt
     1921 ggccatcacc ggattcctgc atggagaact ggatggttgt gatcttctgg ccgtaccgta
     1981 acgcattatc catcaggtta taaaagacct tgacaatcaa tggatcagcg tacacttctg
     2041 cacctgaggg aagattgtaa ttcagcttga cctttcctag tgaggtttcc tttacggctg
     2101 tactaatgat tgtacgagtg tccagccaag aaggagcatg aacaccgatc tcttcgtatt
     2161 ctttagtgaa ccgaatcatt gccgagatgc gctgtgcagc cgcgtttatc ttctggaaat
     2221 attcacgaag ggtgggattg ggctgctttt gttctagaat gctgaggaaa cctctgagag
     2281 ttataagctg gttgaggata tcgtggcggg tgatgcttga taggatattg agtttcctga
     2341 gcgcctgccg gagcgccacc tccgcccgct tacgatccgt cacatcatgg gccacgaagc
     2401 aggagagggt agggcttcct ttttggtgga tgatactgga agtgagttcc acatcccgaa
     2461 gggaaccgtc ccggtgtcgg aactgcacct cccccgacca accccctatc gagcaagctc
     2521 gctcccccca tgccttcccg tcgggtgccg cgatgaggtt gtcgacagtc attccggtga
     2581 gttctccggc agtatacccg agcagccgga tagatgccgg atttgtttca aggattgtcc
     2641 tggtttcgca ggaaatgatc atgatacctt ctgccgcatt gtcaacgatc gtccgataac
     2701 gctgcatgct ctcccggagc cgctgctcaa cggtatgccg gtagagcgcc atgtccacag
     2761 atgcaagcag ctcctgttcg ttgaacggtt tgacaatata attgtagggc atggtctcct
     2821 ttgcccgagc aatccgctgg atgtcggaaa aagcggtcag atagataata gggatattgg
     2881 cgcgctcgtg gatcgtgcat gccgcttcaa tcccatccat tttccccatc agttcgatgt
     2941 ccatgaagat gagatcaggg gaattcttct ctgccatcat cacagcatct tccccatttg
     3001 ctgtcatgcc ggcaacctgg tatccgttct tcttaagcaa ctccgagagc tggagtgcga
     3061 ttattgcttc atcctccacg acaaggattg attcgccggt catggagagc tcataagaat
     3121 cccaagaaat cctctcgtac tttctctgat ggttaatagt tgtatgatca tgatcctgat
     3181 aaccttcgcc ggagccgtgc tgttatgcca tgtacagatc gccttttccc tgcaaggaaa
     3241 accacccttt gccggattaa tcccaatgcg tgatttttcg atataaatat ttcacctgtt
     3301 ttcaaatttt atcacacgcg gggatcattt gaatagtaca gggagatttt ttaaaaagcc
     3361 aatatccaca tcacaccaga atttaggtgt gtttcgcaaa ccagcttaat gatttaaggc
     3421 tccagattcc gaggataatt attacaatac ctgcaataat ggcgacatag ttcaatatac
     3481 tataattccc gggttgaatg ccaacaccga cgatgagcag aatcagacct gcgataatga
     3541 ctacataatt cgtggttttt gcgttcatat aaagacccta ccgttttata atgcaaaatt
     3601 tttattcgat ttcgataccc atcttccccg atctccagaa aaggttcaga ttaaattaac
     3661 gcatcaaggc cccagcaggt caggaaagct gctaatacca gtccttcatt agacagtgga
     3721 aaaaactttg caaacgataa tacgataaca atcactccga tgataactgc taatattcgt
     3781 gccaattttg taatttttgt catgcttttt ctccttcctg ttggtttgct ccaacagtca
     3841 cctcaatcgt tctctttctt cttatttttt cccctgaatt atcaaaatta taaagataag
     3901 gttgtgaatg ccaccattac tcttgtgaag cgataaggtg gataccgatg ggtgtatcct
     3961 gttactctgt accataccga cgttttttat gctatgaaag atggtatgaa aaaatacgaa
     4021 cggtatcttg gcagacttat tttcaatcag ttctcaatat ctgttttctc aatacctatt
     4081 ttttcagact tgtgtcacat tttgtaatgt ccgggcaggt atacgtaatc agccgtggga
     4141 ttagcctgca tgccatccaa agatgatata cgagtcaagg cccacaaggc tgttgacaac
     4201 gcccgtgtag cggtgaatga agtagtcgat gatgcgaaga cggcggttca caatgccaca
     4261 acgcgcaaag gtacagttgt gcaccacccc acggctcaga aaacggtcga tgataaaaag
     4321 atcgaagcac ataaagtcag ttcagatgcg aaaatcagaa cgcaccggaa agcagaagaa
     4381 gcaaacaaga agtagccgta cattgagcga gcacatgaca gagatacttc cacgttccag
     4441 atgagttgtg ggcactgaca aaggagatta aggagacaaa agctgcagga gaagcgagga
     4501 cgagcaatgg agtttccaac aacgacctct cgtccgacct aatcaattcc ccggcgttgg
     4561 aaattctctg gcagaaaaaa ggaaagatct tctgcaactg catatcctcg agaaatggtg
     4621 aatgcatcca gatccgaaaa ggaggaaaat atcttgttcg cattcagtta gtagccttct
     4681 cttgactcga gggtatatat tgatcacgac gaaccgactc atggttcagt atgaaaattt
     4741 gtcctttcag acaatcgcga tttgaacact caatatatct ttgtccgaat tctattattt
     4801 cccatactgg ttgcttcgga tctggtattt tacctctccc tttacatatt ttacaagcag
     4861 tccatggcat aaaaatcccg aaaagaggat ttgtggattt atattatttt aacttatcga
     4921 tattgttctg ttggcagatc tcccaggctc gcgtatgttt ttatgcggct aaatttgaag
     4981 gattacttaa ttattatttt taattttatt cattgttaac agtgtatttt taccagaaaa
     5041 atggttttca acagatcacg tatttccgga aacctccccg caaccttctt ctttctatcc
     5101 tccccaatac accgtaatga catctcccga tggccctccc gacccgttct ttgccagaga
     5161 tgcctgggca caggaagcaa atatcgagct catggaagta tcggccggaa gggccaaggt
     5221 ccggatgagg attgccggct ggcacatgaa cagtcacgga actgtgcacg gcggtgcaat
     5281 cttcacgctg gctgacaccg cttttgccct tgcctccaat tcccacgggg tccctgccgc
     5341 ggccatcaat gcccacatct cgtatgtaaa agcggctaca gccggtaccc tgtacgcaga
     5401 tgccgaagaa ttttcgttaa accctaaaat tgccacctat atggtgacag taaccgatga
     5461 tgccggagag aagattgcga tctttgaggg catggtatac cggaaaacac cgggccggga
     5521 caagtgacga aagaccgcaa aaccggatca cccgttgttg tgaagggtgc ttcatttttc
     5581 ctgttgaatt aaaaaagaaa atcaccgtgc agggacctgg aaatccctga ctgtttctgc
     5641 catctgctgt aaccttttgt tgaccaggta atttaccgtt ccctctttat acgatccatc
     5701 cgccagcggt tctcctgcag gtactccggt aagaaattca ataccctcgt caatggtctt
     5761 taccgggtaa acggtaaact tccctgcttt agccgcttcc aggatctctt ctttgagcat
     5821 gaggttctgg acattgcttg ccgggatcat tgctccctgc ttcccggtca gacccctggc
     5881 cttgcatacc tcgaaaaatc cttcgagttt ctcgttcaca cccccgatag cctgcacttc
     5941 accccgctgg ttgaccgatc cggtgactgc aatggactgc ctgatcggca gacctgagag
     6001 ggcagaaagg atcgcgtaca attccgtgct cgaggcgctg tcaccatcaa ctcccccgta
     6061 actctgctca aagatgagtt ttgcggcaag cgagagtggt ttgtcccggg catacctgtc
     6121 tgcgaggtaa cctccgagga tcagtacacc ctttgtatgg gtgggtcctc ccagttccgc
     6181 ctgccgctcg atgtcgacga tcccttcacg gcccaccgca acactggcag ttacgcggga
     6241 tggccaaacg caacatcgcc catctccatc accgagagcc cgttgatctg cccggttttg
     6301 ctcccttcgg tatcgatcag gaatactccc cgcgcgataa acacctgtat tttttgctgg
     6361 atcaggttgg aacggtacac cttttcctcg accgctttta agatatgttt cacggttatg
     6421 tagggtgctt tttcctgtat tgcgtaaaag ttggcctccc gtataaggtc agcgacaccg
     6481 gaaaatcttg tggagagctt ctttttgtcg tctgcaacgc gtgacccgta ttctaccact
     6541 tttgccacag cggtattatc caggtgcttt aaactgaagc gtttacagag agtacagatg
     6601 aacgcggcgt atttcttggt attttcttca ttcctgtcca tgacggtatc aaaatccgcc
     6661 cttaccttga agagttccgg gtagtccggg tctccctggt ggaggatctg gttgatatcg
     6721 ggtgtgccga taaggatgac tttcacgctc agcggaattg gctggggttt gagccctctc
     6781 gttgtaatga accccgcctg ctcccccggc tcttcaatcc ggatcttctc tgttttaagg
     6841 gcggatttga gcccgtccca ggctaacggg tacctgaaga ggtcaagtac cgggatgacc
     6901 aggtaccccc cgttggcttt atggaccgat cccgggcgga tcatcatgaa atccgttgtt
     6961 actacgccaa actggacttc ccgttctgct ctccccagga ggttagggta gctgggagtc
     7021 tcttcaaaga cgaccggggc accggcaata ctaccattgt ccacgacaac attaatctcg
     7081 tatttcctga atgcgaggtc ttttgcgagc cactgctgga acgggggcgg gacctgttcc
     7141 tgttgctgct gctgttgctg ctgtgccgat ccaaggaata tgctgaggtt gtccaggatg
     7201 tctttctgga cgctgtcgat gtacgggagc acgtcactga tttttccgta tttttctttg
     7261 agacccgcaa gaagatggcc aatggcatag agcgctatct cgctgttgag ctgggtgacc
     7321 acctccatct cttttgctcc aagctcccgg acctggcgaa aaccgctccg caggtcaata
     7381 tcaagggctt cccttctctg gagtattccc gcctgggtct cctttgggag cgcttcaaac
     7441 tcttcctgtg taaggggctt acccccgacg accggaataa tgatgagtcc ggtgggcccg
     7501 atctggatcg taaaaccctg ttttgccgca ctttcattga cctgcccgac gacgtcatcc
     7561 cgctctttat tgagtttccc gagcgcttca gaccgttttt ttgcataatc atcactgtca
     7621 aatgcacggg gcagggctct tcgagcttct ttgataaaga atgccatgtc tttcttcagc
     7681 tggctgctca tcccgggagg aagtcggatc gcgagcggct catacgggtt ggtaaagtta
     7741 ttgacataga cccagtcatc ggcttttggt ttggttttcg ccagctcgtc aaggaaattc
     7801 ctgaccgcag tccttctccc ggttccgggc atcccggcca cataaatatt aaacccgcgt
     7861 tcctggattt caagaccgaa ccggagcgcc ctttgtgccc gttcctgccc gataatctct
     7921 tcggtgggtc tcatggtctc ggtagtagga cactcaacct gacttgccta gtaaacggcc
     7981 cggtactctt caaggggtaa tgctttgatc atgtctggtt ctcctcatgt tttcatcgtt
     8041 taagatcagc caaaatatcg tttaaacggc aacccgccgg gggtggtata tacgcaggat
     8101 cttcatgaga tccccttgat aataccgtgt ttttgttcat agttcctgac ggcccccggc
     8161 gccccggcaa gtaagcgtcg tatggtgtta tag
//
LOCUS       DASRLV010000163         4118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_873025,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000163 DASRLV010000000
VERSION     DASRLV010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873025"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1665
                     /locus_tag="VFC43_06420"
     CDS             <1..1665
                     /locus_tag="VFC43_06420"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HZK30849.1"
                     /translation="WDFGDGSPLSTESTEVHTFTETGKEYNVSLTAINAYGASTSTKT
                     IRTLMGARSRAATPVNGIMVDRRFKGQFLTYDPSVLPLFSPDRPTTYLTVYPSPDYGW
                     QNITFRSSDKSGVHLNPSENTYHTNLSRIYLTTTDTIATTTGTIPRLGNNWSVSYRIN
                     TTVFPTESSFETVTRESASESDRKIFDDIASRVPPSGTLVRNIAYTATFTRKNIRNEG
                     IALINMSVSEDWVKGPDPNTTIAEGRDLTYIMGYWYDKTGNRIGGILSKRYVTTSDGM
                     DYYEAEIPESAGDQSTFALVKLSGSGNPIQMISFLLAQVINPQYAGGGNKPEVVQTVV
                     TPEIKPTVTPDPGKTAKIYTNAQGIITQATTLTSNDGFASISLGLGVVATDTNSSPLS
                     SISITRISGENLPGASPKTALSAAGMFYEIQPDGVTFSPPVPVSFTIPHAQWGYEYVI
                     QEFDHTTGTWQVLPGSYDPKTGIITTHVSHLCCFALFTKAPVVENTVSPKPTKTLLVA
                     SKSSISTNVEMYSWIICSIQQNPVIIVIALAVLAVVAYFGWWRRRL"
     gene            complement(1753..3267)
                     /locus_tag="VFC43_06425"
     CDS             complement(1753..3267)
                     /locus_tag="VFC43_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HZK30850.1"
                     /translation="MKSLEHIVMIAHDVGLIFEFLGLASLLPFLVLIIFREWDMLLPL
                     ATAPLIFIVFGGLISHISYKDLEPSFSGTLLAVAFSWFAIALIGALPFMFGLHMSFTD
                     SVFEAMSGWTGTGFSMITSLDTTPKTLLFWRSFTQWIGGIGIIAFGISLRRKTRVSLF
                     RLYRAEGREEELVSPVTSTSRRMWMIYLVLTFGFTGFIMLAGIPLWDSLNLVMVAIAT
                     GGFTVHSGGLGYYNNPLLETMLIPVMFAGAIPFKLFFFLYHGKIREMFRDKTVKIFML
                     IAVAGSLLTSWDLYIFSNLSFSTALRQGTFSAVSAICTCGLQNSDPHYWVGIPIAVVG
                     MMMFIGGTMGSAAGGIKVNRVILAYEGVRWWFRRFFVSSNVLIPLRFGGKNLSKEISE
                     LAISKNMLVIVVYVLTIFVATIVSLHLYITSFRLDEVIFELVSALSNVGLTVGFISAA
                     SPFSIKWIFIILMWLGRLEIVPVIIIVMGFIKGIEMDITQQKPAPTIYDHQERY"
     gene            3393..>4118
                     /locus_tag="VFC43_06430"
     CDS             3393..>4118
                     /locus_tag="VFC43_06430"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZK30851.1"
                     /translation="MKSNRTDLFPFVLIIVLTLISFVIFWSVMDMVLLGASIAVVLLP
                     LHHRILKYTRPRISAAIVTLAVLIALSVTVLVTLFILNSNAGTLRELFGAIGIWLNNP
                     ATNPLDFGIPVTKATLSTMLAEGNALFVDYQKTLINYLYLIMFKMLVLFVSIFSLLRW
                     GEELEIKLTRHVPEILKGYIRKLAEVTSDTLYAIYIVQIAIAILTFFIALPVFYLLGY
                     GNIIFYSFLAAFCELIPILGSSVA"
BASE COUNT         1157 a         1005 c          922 g         1034 t
ORIGIN      
        1 tgggactttg gggatggcag cccgttatca acagaaagta ctgaagtaca caccttcact
       61 gaaacaggaa aggaatacaa tgtatcttta accgcgataa acgcatacgg cgcttcaaca
      121 tctaccaaaa ccatccggac cctcatgggc gcgaggagca gggctgcaac accagtcaat
      181 ggtatcatgg ttgaccggcg tttcaaaggg cagttcctga cgtatgaccc ttcagtcctg
      241 cctttatttt ctcctgatcg gccgacaacg tacctgaccg tttatccatc gccggattac
      301 ggatggcaga atattacatt ccggtcatcg gataaatcag gtgttcatct gaatccgtcg
      361 gagaatactt atcacacaaa cctgtcacgg atctatttaa caacaaccga tacaatcgcc
      421 accacaaccg gcacaattcc ccgtttaggg aataactgga gtgtcagtta ccggattaat
      481 actactgttt tcccgactga aagttcgttc gaaaccgtaa caagggaaag tgcatcagag
      541 agtgatcgta aaatatttga tgatatagca tcacgtgtgc caccttccgg aacccttgtt
      601 cggaatatcg cgtatactgc aacttttacc aggaagaata tcaggaatga gggaattgca
      661 ctcatcaata tgagcgtcag cgaggactgg gtaaaaggac ctgatcccaa cacaaccatt
      721 gccgaaggcc gggatttaac ctatattatg ggttactggt acgataaaac aggaaacagg
      781 attgggggta tcctgtctaa acgctatgtt actaccagtg acggcatgga ctactatgaa
      841 gcggaaatcc cggaatctgc aggggatcag tcaacctttg cattggtgaa actctcgggt
      901 tccggaaacc cgatccagat gatctccttc cttctggcac aggtaatcaa cccgcaatat
      961 gcgggggggg ggaataaacc ggaggttgta cagacggtgg ttacgccgga aattaaacct
     1021 acggttaccc cagacccggg aaaaaccgct aaaatctaca caaacgctca gggaatcatc
     1081 acccaggcaa ctacgctcac ttcaaatgat ggttttgcat ccatctccct tggtcttggt
     1141 gttgttgcaa ctgataccaa tagcagtccc ctctcctcga tcagtattac acgaatttcc
     1201 ggggaaaatc tgcccggagc ttctccgaaa acagctttat cggctgctgg tatgttttac
     1261 gaaatccagc cggatggagt gacattttcc ccgcctgttc cggtgtcatt tacgatcccg
     1321 catgcacagt ggggatatga gtatgtcata caggaatttg atcacacaac gggaacatgg
     1381 caggtcctcc ccggtagtta cgatccaaaa acgggaatca tcacaacgca cgtttcgcat
     1441 ttgtgctgct ttgcgttatt cacaaaagca ccggtagttg aaaataccgt cagcccgaaa
     1501 cccacaaaaa cacttctcgt ggcctcaaaa tcctccattt ccaccaatgt tgaaatgtac
     1561 agctggatca tttgctcaat acaacaaaat ccagtaataa ttgtcatcgc gttggcagtc
     1621 ctcgctgtag tcgcatattt cgggtggtgg aggaggcggc tgtaatacag gagtgaaaaa
     1681 ccctccgtgg cagttgagag gtaatatctt atccgttatt gcccggacac ggatacaatt
     1741 atttttcctg tgctaatacc tttcctggtg atcgtatatc gtgggagcag gtttctgctg
     1801 ggtgatatcc atctcgatcc ctttgataaa tcccatgaca ataattatta ccggaacaat
     1861 ctcgagtctg cccagccaca taagaatgat gaagatccat ttgatagaaa aaggactcgc
     1921 tgccgagatg aacccgaccg taagacccac attactcagc gccgagacca gttcaaatat
     1981 gacctcatcg agtctgaagg acgtgatgta gaggtgcagg gatacgattg ttgctacaaa
     2041 tatcgtcagg acatagacaa cgataacaag catgttcttc gagatcgcaa gctctgaaat
     2101 ctcttttgaa aggtttttgc ccccaaaacg gaggggaata agcacgttgc tgctcacaaa
     2161 aaaccggcgg aaccaccatc gtactccttc ataggcaagt attacacggt ttaccttgat
     2221 acccccggca gcacttccca tggttccccc gataaacatc atcattccga caacggcaat
     2281 cgggattccc acccagtaat gagggtcaga gttctgtagt ccgcaggtgc aaatcgccga
     2341 tacagcgcta aacgttccct ggcggagcgc agtagaaaat gacaggttgc tgaaaatata
     2401 caaatcccag gatgtcagca acgatcctgc aacagcaatc agcatgaata tttttacggt
     2461 cttatcccgg aacatctcac ggatttttcc atgatagagg aaaaagaaga gtttgaacgg
     2521 gatggcacct gcaaacatta cgggaataag catggtttcg aggaggggat tgttgtagta
     2581 tccaagtcct ccggaatgga cagtaaaacc ccccgtggca atggcgacca taacaaggtt
     2641 cagcgaatcc caaagcggaa ttcccgcaag catgatgaac ccggtgaacc caaacgtcag
     2701 cacaaggtag atcatccaca ttctgcgact cgtggatgtt accggggaca ccagttcttc
     2761 ctcacgtcct tctgcacggt acagccggaa caatgagacc cgggtttttc gtcgcaatga
     2821 aatgccgaat gcgataatcc ctattccacc gatccactgg gtgaatgacc gccagaacag
     2881 aagtgttttt ggcgtcgtat caagcgaggt tatcatggaa aagccagtcc cggtccagcc
     2941 ggacatagcc tcaaaaacac tgtcggtaaa ggacatgtgc agcccgaaca tgaagggaag
     3001 agcaccgatc agggcaattg caaaccagga gaacgcgact gcaagaagtg tacccgagaa
     3061 ggaaggttcg agatccttat aagagatatg tgaaatcagt ccgccaaaaa caataaaaat
     3121 gaggggggca gtagccagtg gcagcagcat atcccactca cgaaaaataa tgagcacgag
     3181 aaaaggcagc aatgacgcga gaccaaggaa ctcaaaaatg agacccacgt cgtgcgcgat
     3241 catgacgata tgctcgaggc tcttcatcag atattcaagc tgattggtct ttttatattg
     3301 ttatgggtat cgctcttttt taattgacca tattttttta aagggacagg cacacctatt
     3361 aatttctggc acggcacaat acgtattcat ccatgaaatc gaatcgaacc gatctgttcc
     3421 cgttcgttct aattattgtg ctcactctta tctcatttgt gattttctgg tctgttatgg
     3481 acatggtact cctgggcgcc tcgattgctg ttgttcttct gccactccac caccgcatat
     3541 taaaatatac caggccaaga atctctgcag caattgtaac tctcgcggta ttaattgcct
     3601 tgtctgttac ggtcctggtc acacttttca ttcttaattc aaacgccggc actctcagag
     3661 aattgttcgg agccatcggt atctggttga ataacccggc gaccaacccc ctggattttg
     3721 gtattccggt aaccaaggca accctgtcaa caatgctcgc ggagggaaat gcactctttg
     3781 tggattatca aaagaccctc attaattatc tctatcttat aatgttcaaa atgctcgttt
     3841 tatttgtttc aattttttcc cttttacgct ggggggaaga actggaaata aaactcactc
     3901 gtcatgtacc ggaaatattg aaaggctata taaggaagct cgctgaagtg acatccgata
     3961 cactgtacgc aatatatatt gtgcagatcg ctattgctat cctcacattc ttcattgctc
     4021 tgccggtttt ttatctttta ggatatggaa acatcatctt ctattcattc ctggctgctt
     4081 tctgtgagct cattcctatc cttggatcat ccgtcgcc
//
LOCUS       DASRLV010000164         2892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_57559,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000164 DASRLV010000000
VERSION     DASRLV010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57559"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..544
                     /locus_tag="VFC43_06435"
     CDS             83..544
                     /locus_tag="VFC43_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZK30852.1"
                     /translation="MNKNEKILKWSRPVEILLVDDSPADVALTQEAFSDNKLCNNLHV
                     VNDGVEALEFLHKMGQYASAPTPDFILLDLNMPRKDGREVLAEIKADDELKYIPVVIM
                     TVSKDDKDILESYRLHANCYVKKPVKFTEFVEVIRSLENFWFSVVTLPSKK"
     gene            556..1608
                     /locus_tag="VFC43_06440"
     CDS             556..1608
                     /locus_tag="VFC43_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HZK30853.1"
                     /translation="MTILLIEDNPADVEMIRELLSDRKGSFFDIVCADRLSAAKPYFA
                     AGGIDIILLDLGLPDSQGLDTLRRVRDHAREVPIVVLTMLDNEETGLNALKEGAGDYL
                     VKGQMNGPLIARSLRYAIERGRIEKELKKNRDSLEELVAEKETLLREIHHRVKNNLQL
                     ISGLLDMTLMSSSDESTNSILTDIMLKIQTMAQIHTRLYESKQIGKINITDQCRDQVT
                     ALSNIYSHTGHEIQCTINSDELFLPVDKALPCALVINEILSNAYKHAFKGRECGTIEI
                     SVVQEDERIKIIIRDNGVGLPDNFDIGRSGSLGITLIRTIVQHQLKGSLLFASQHGTK
                     ISIEFPVIPGGTSGGT"
     gene            1612..>2892
                     /locus_tag="VFC43_06445"
     CDS             1612..>2892
                     /locus_tag="VFC43_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZK30854.1"
                     /translation="MSKILVVDDEAIIILQLEKRLSAMGYTIAGMAASGEDAVDMARH
                     ILPDLVLMDIMMPGKMNGIEAAKIITGELDIPVVFVTSYADDSIIEKAKSVRPYGYIV
                     KPFNELEIKAAIEVALFRKTTEQEEQRLRKSAQEKISPGLETGTLAQDGLAYVDLPEI
                     KSILLKDIFTDIVLFLYTDQVEKDPIFKFALEEGIKKGGRNLFAYFQSTLIKYFPKEI
                     QKKEIFTHRIKRNEVYTLLPVLERCMESLMNAGTPTSLQFLLDFSENEEFEDILVIRK
                     LLLVKKEKGIPLSGIIAMNIGKIDHTLIKSLSEGIPRIIVSTGKETTLSFAQHTFPPG
                     SVSVVPQSTVEDVVKKSLEPVVLSLLEKPISGYDIVREIHSRYKVLIPQARVYTILHD
                     LMEEGYLEIRVSGKSKLYCTTEKGKIHVSQKLKDF"
BASE COUNT          866 a          644 c          680 g          702 t
ORIGIN      
        1 ggtactatct ggatcgagtc tgttcccggt tcgggatcga cgttttattt caccattcca
       61 aaaagaaaga aggaaacgaa ctatgaataa gaatgaaaag atattgaagt ggtcacgacc
      121 ggtcgaaatc ctgctggtcg atgacagtcc agctgatgtc gcacttacac aagaggcgtt
      181 ttcggacaac aaactctgca acaacctcca tgtagtgaat gatggcgtgg aagcgttgga
      241 atttctccat aagatgggtc aatatgcctc ggccccgaca ccggatttca tcctgcttga
      301 ccttaatatg cccagaaagg acgggcgtga agtccttgca gagatcaagg cggatgatga
      361 actgaaatat atccctgtcg ttatcatgac tgtctcaaag gacgacaagg atatccttga
      421 atcataccgg ctccatgcaa actgctatgt aaaaaaacct gtaaaattta cggaattcgt
      481 ggaggttata aggtcacttg aaaacttctg gttctcggtt gtaacactcc cgtcaaaaaa
      541 ataaggtccc gtgtaatgac gattcttttg attgaggaca atcctgccga tgtggagatg
      601 atacgtgagt tgctgtctga tcgaaaagga tcttttttcg atattgtttg cgcagaccgg
      661 ttatcggcag caaaaccgta ctttgcagct ggcggcatcg atattattct tcttgacctt
      721 ggacttcctg acagccaggg acttgacacg ctccggcgtg taagggatca tgccagggaa
      781 gtaccgattg tggtactgac catgcttgat aatgaagaaa ccggtttgaa tgcactcaaa
      841 gagggtgcag gggactatct tgtcaaaggt cagatgaacg ggccattaat tgcccgttcc
      901 ctgcgctatg ctatcgaacg aggcaggatc gaaaaagaac tgaaaaaaaa ccgcgactct
      961 cttgaagaac tggttgccga gaaagagacg ctgcttcgcg agatccacca ccgtgtcaag
     1021 aacaatctcc agctgatatc cggcctgctg gatatgaccc tgatgagttc ttcagatgaa
     1081 tcaacaaaca gtattctgac cgatataatg ctcaaaatac agaccatggc gcagatccat
     1141 acccggcttt atgaaagcaa acagattggg aaaatcaata tcacagacca gtgcagggac
     1201 caggtcacag cattgtcaaa tatctattct catacggggc acgagatcca gtgtacgatt
     1261 aattcagatg aattatttct cccggtagac aaggccctgc cctgcgcact ggtgatcaat
     1321 gaaatcctgt cgaacgcata caagcacgct ttcaaagggc gggagtgtgg cacgattgag
     1381 atttctgtag tgcaggagga cgaacggatt aaaatcatca tccgtgataa tggagtcggg
     1441 ttacccgata attttgatat cggccgttcc ggcagtcttg gaatcacgtt gatcaggaca
     1501 attgtgcagc accagttgaa aggctcacta ctatttgcga gccagcatgg tacaaaaatc
     1561 agtatagaat ttccggtcat ccccggggga acgtcggggg gaacgtaaat tatgtcgaaa
     1621 attcttgttg tcgacgatga agcgatcatc atattgcagc tggaaaaacg gttaagtgca
     1681 atgggatata ccattgcagg catggcggct tcaggggaag atgctgtcga tatggcgcgg
     1741 catatccttc ctgaccttgt cctgatggac atcatgatgc cggggaagat gaatggtatt
     1801 gaagcagcaa aaataatcac cggtgagctg gatattccgg ttgtttttgt cacatcgtat
     1861 gcagatgatt ccataattga aaaagcaaaa agtgtccgac cttacggtta tattgtaaaa
     1921 cctttcaatg aactggaaat caaagcagcc attgaagtgg cactgttccg gaaaacaaca
     1981 gaacaagagg agcagagatt aagaaaatcc gcacaggaaa aaatttcacc gggactggaa
     2041 accggcactc tggcacagga tgggcttgcc tatgtggatc tgccggaaat aaaatccata
     2101 ctcttaaaag atatcttcac agacatcgtc ctcttcctct acacagacca ggtcgaaaaa
     2161 gacccaatat tcaaatttgc actggaggag ggcataaaaa aaggaggacg caacctgttt
     2221 gcctatttcc agtcaacgct cataaaatac ttcccaaaag agatccagaa aaaggaaatt
     2281 ttcacccacc ggataaaaag aaacgaagtt tataccctgc tgccagtcct tgaaaggtgt
     2341 atggaatctt tgatgaacgc aggtacaccc acttcactgc aattcctgct ggatttttcc
     2401 gaaaacgaag agttcgagga tatcctggtc atccggaaat tacttcttgt taaaaaggaa
     2461 aaaggaattc ccttatccgg gatcatcgcc atgaatatcg ggaaaatcga tcataccctg
     2521 ataaaatcct tatccgaggg tattcccagg atcattgtat caaccggcaa agaaacaacg
     2581 ctttcgttcg cccagcatac gtttcccccc ggatctgttt ccgtagtccc ccagtcaacc
     2641 gtcgaggatg tcgtaaaaaa gtcacttgaa ccggtggtcc tttctctcct ggaaaaaccg
     2701 atttccggat atgatatcgt tcgcgagatc cattcccggt acaaggtcct gatcccgcag
     2761 gcaagggttt acaccatcct ccacgatctt atggaggagg ggtacctcga gatcagggtt
     2821 tcaggaaaat ccaaattgta ttgtactacg gaaaaaggaa aaatacatgt cagccagaaa
     2881 ctcaaagact tc
//
LOCUS       DASRLV010000165        16210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1025867,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000165 DASRLV010000000
VERSION     DASRLV010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 16210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16210
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025867"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VFC43_06450"
     CDS             <1..238
                     /locus_tag="VFC43_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HZK30855.1"
                     /translation="YYSRSRKKLWKKGEESGHFQKVVSILTDCDEDCLLYLVEQQGAA
                     CHTGFESCFYRTLEGNVVGKKVFDPEKVYRKKGH"
     gene            354..2801
                     /locus_tag="VFC43_06455"
     CDS             354..2801
                     /locus_tag="VFC43_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HZK30856.1"
                     /translation="MDPSAAIRDLKGLSEQEALEQLTKSGLNRMTSRHEITFFGIAKE
                     EITEPMILLLLAVGVCYTILGNLNDALTLYAIIATLVLVEIANEYRAKKAISSLALLA
                     EPKTRVVRDGSIKEIETGFVVPGDTLVIVSGTRIAADGRVLFAVSLQVDESSLTGESF
                     PVEKVTGDEVSGGTLVVAGEGMMEAVSTGGSTRIGKLAALAQEIRQPKTPLQLAMKAL
                     VKTLVFVALFFSISIHLIGFLQGQPLREMFLIGLALAFATIPEELPIIITMNLGLGAY
                     MLSKENFLVKKLKAAETLGNATVIVTDKTGTLTESTMHVASVFPENNTDSILRSARSA
                     MTDISDSITDRAVITAAEIHHISPDPGLVIRERSFETGKKTRALIRDQNGSQVMYLIG
                     APEEILAGVTGETKEAAAALAKETERGRRVIAVAHRAISSEEREIPLPALERDLILDG
                     LIAIEDPPRPGVRETIERAHRAGIRIIMVTGDHPQTATAIAGEVGIPHDRVLEGKDID
                     ALGDTDLRTTLAEVSVFARATPQHKYRIVKNLQEKGEVVAVTGDGVNDSLALKAADIG
                     IAMGIKGTDAAKEAADIVIADDNFITIGKGIFEGRRIFDNLSKGVKYYLSVKVALIAV
                     FAASLAAAIPFPFSPVQIILLELFMDLGASASFVAEPPEPSIDQRPPHDPKIPFLNSP
                     MLAGIALGGAALWVAVFIPYWSALYLGIPLAAAQTAAFSSWMIGHILLAFFSRSAHDP
                     LYRTGVFSNRVMVLWAGGASAFLALLLTVPAIGQRFGITPISAGTVLVIAGFALSCMA
                     GFELAKFFKKSEKMTTG"
     gene            complement(2838..3059)
                     /locus_tag="VFC43_06460"
     CDS             complement(2838..3059)
                     /locus_tag="VFC43_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HZK30857.1"
                     /translation="MNVSIDRGGCVSCGACWNICPEIFGQNPCDSFSEIVEEYRFSCN
                     RAEGIVPDDLCCCAREAANLCPVQVINIC"
     gene            3340..5301
                     /locus_tag="VFC43_06465"
     CDS             3340..5301
                     /locus_tag="VFC43_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PINc/VapC family ATPase"
                     /protein_id="HZK30858.1"
                     /translation="MILVPDTSVLIDGRITSMIKAGEYKGATIIVPEAVIAELEAQAN
                     NGREIGFSGLNELQQLCRMAEEKTIELKFSGIRPSLEQVKLASGGEIDAMIRNIAIEN
                     SARFITSDFVQAEVARAKGLDVIYLKAQVSDFVPLGIDQFFDEHTIAVYLKERVCPSA
                     KKGTINDMKLVKIREQPTSEYELRALAQEILERAKRDPDGFIEVEKRGITVVQIGSMR
                     IAIARRPFSDGMEITAVRPIVDVTLKDYAKADLITKRITSEKRGLIIVGSPGSGKTTL
                     AQSVATYLSDSGYVVKTMEAPRELQVPDQITQYTMLDGSMSNTADVLLLVRPDYVIFD
                     ELRKNEDFNVFADMRLAGLGMIGVIHANGVQDAVQRFSDRVDFSVLSQIVNTIIFVKQ
                     GVITNIYDVGFTIKVPEGMANEMHIRPVTIVNDHETGELVIDVFRYDGETIVMPMSAV
                     ANAGKKVTPPVPVQNVGVSVAATGNVRSPVPLPELSPKKENEERPGWKIMEKDIQREL
                     GRYTEGFVDVQMISDTKAIAYIDDKDVPAAIGKGGKNVSAIVNKIGIGIDIKPHSEFD
                     RMQIQPQKTDMELNIGGGIKIQTDKKQLTIVAPEQSGKIVDVFAGKEYLFTATVNDTG
                     EIHLSKNSSIAQEMIRRYQNSEIIKLRPV"
     gene            5349..8129
                     /gene="leuS"
                     /locus_tag="VFC43_06470"
     CDS             5349..8129
                     /gene="leuS"
                     /locus_tag="VFC43_06470"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HZK30859.1"
                     /translation="MNSFDLQKFEEDAQEQWNHAFESNPSDLEKFYLTVAYPYPSGAM
                     HVGHGRTYIVPDVIARFHRMQGKQVLYPMAFHVTGAPVIGISKRIAKNDPKTIHLYRD
                     LYKVPQNVLDEFTDPLTIVRHFASEYQRVMTACGISIDWRRRFTTVDPTYSKFIEWQW
                     KYLFEAGHVIKGAHPVRYCTVDDNPVGDHDLLEGDKAEVIKFTLIMFRYGDAFIPTAT
                     LRPETIYGVTNLWANPHVTYVRALVDGIPWIISQEAAEKLALQDHTVEVTENIAGQDL
                     IDKTVDHPLCGTVPVLPADFVDPDMATGLVMSVPAHAPFDYIALRDLQQAGKYTTIRP
                     VPLIRVEGYGDIPAKDAVERAGISHQMDSRMDALTQEIYSAEFSKGKLYEKYGGKPVR
                     FARDEVALLMIDQYESEIMYEFDTRPVVCRCGNRVKVKILHDQWFLKYSDPAWKKQVS
                     DHLGDMALVPPEVRLEFDRTVDWLKDWACTRRVGLGTRFPWDTTQLIEPLSDSTIYMA
                     YYTIAHKIREIDPKLLKPEVFDYIFLGKQSPELPEKKKLDAMRKEFLYWYPYNFRFSA
                     KDLISNHLTFQLFHHVTIFPKDKLPQGMVVFGMGLLNGAKMSSSKGNVFLLEDAVSDF
                     GADTVRMFLMGSAEPWQDFDWRNELVISTKKQIERFFNTVGESKDADGEPHDIDHWII
                     SRLQSHIERTTEALANFQTRQALQEAYFGIETDLKWYRRRLFENCDGSRELHTLCSVW
                     VRLLAPFIPFTCEHLWKEIAGEGLVSFAPWPVPDKKLVNTRIELAEELLSRTVEDIES
                     IMKLIQITPKSVTISVAPAWKQEIFLHIARAEDRTNVIRDIMKDDAMRKRGREATDAA
                     KQCTTLIHRLPPHVVGHLAKEPVNELAVFQAAKSFLEKEFGVPVHVTQAESSEHAKAA
                     TALPFKPAIVIE"
     gene            complement(8199..8369)
                     /locus_tag="VFC43_06475"
     CDS             complement(8199..8369)
                     /locus_tag="VFC43_06475"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HZK30860.1"
                     /translation="MPSFMCKDIGIDCPFEAHGSTDHELMRKFIDHAEPAHKMQVLSA
                     DVIFKVRNAIRK"
     gene            8562..9818
                     /locus_tag="VFC43_06480"
     CDS             8562..9818
                     /locus_tag="VFC43_06480"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZK30861.1"
                     /translation="MNTRFRINLVITGFLLAICIIGTVTAASVAHTWQEQPAFDGSFS
                     GLMFSSDGSMIFAGGSQLLVRSWNGDIHWGGRTGTVAAMNSDGTRVISALDSSVRVLD
                     KNGEEVWTRILGSSPIRAVAVSRDGSLILGANDEGYIQAWDVNGTKGGLNKTDRVKKI
                     AISPSQTLIVAATEAGLKFFSPKMEQIWADDKEGTLDSFIAFSADSSTVITSGDKRVW
                     SHTGTGKLNWMREVTQTGITDMACSDDLTAIVLGRKEGDVLVLDDTGQERWKYPAGSW
                     IEGVGVSSDGSIIAAGALDGTLYILDRNGNLLLKTTTGTLIQQESVLVSRDGKRIMVA
                     DEGTLYGFDLLGLPEVTPAVTQSPTPEIPSTCPTCPACPAPVTTASPARTTVPSTTGT
                     PAAPLNPYLGFIACAGGALMVLRRKT"
     gene            complement(9864..11552)
                     /locus_tag="VFC43_06485"
     CDS             complement(9864..11552)
                     /locus_tag="VFC43_06485"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZK30862.1"
                     /translation="MSYCPECGKRVAPTAKFCRNCGASQLEESPSNPPPAAVPEVKRT
                     CGSCSNPLSQDEMICGVCGAREGEIRPQVIPAAPPVSPPPSPAPVPSPSLSCTLCGMP
                     LNPGTRFCGGCGSPVKGDGIPAPLTVPAVITASPSPQSQYIPPPAPQPPQQAGIRVCR
                     SCGYVINPGDKFCGKCLAKVVDSTLESPVPVKVAVPPVSPPPPPPVIPDFGAGTSSTQ
                     QAGMRLCRSCGNVINPGDKFCGKCLAKVVDSTLESPAPLKAPVPPVSPPSSPPSPLPD
                     HGSETIPAQHQGVRLCRSCGYIINPGDKFCKKCLAKVVEDYPVAPPQTRQSTGDIAAS
                     SVTPPVCKSCGSPLGGIEKFCGICGIPVGAGASSGTIPPAATAPFVPTQGAQGSAVAT
                     GEEVMGVIANARKMKMFGASWDTYTIVVTNRRMILAQMTQSLLNAAIMEAQAKAKAEG
                     KGFFAIVKDQMAAQFRFALRYETMPPDQSLAETAGNFAIENTQITAITLKLNDSGEME
                     YTEFKIIIESSGGKFEYLIGEDDRFINLLKTVYGERVHMPFGYFRAGGVRLKFF"
     gene            complement(11787..12476)
                     /locus_tag="VFC43_06490"
     CDS             complement(11787..12476)
                     /locus_tag="VFC43_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30863.1"
                     /translation="MTLCETCKTEVPEPQRFCTACGTALASSPENDGSGDTKLTAGTS
                     GELVIWNRKIPLITNPYLVLQCILIPLGIAIVMGGFLSLVTGSVEMLVLFFVISAGLS
                     VLMILIMLVLQLVTGGGLDTEFLISNKGVAHNAGSTTRALNRASAGGSAMLGSLAGTG
                     AGLSAISQENTVLPWEDVRYISVYPRTQSIVFRSRYLIGPVVLYCSEENFLPVLAMVK
                     KYAPQVAKNNL"
     gene            complement(12487..12801)
                     /locus_tag="VFC43_06495"
     CDS             complement(12487..12801)
                     /locus_tag="VFC43_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5611 family protein"
                     /protein_id="HZK30864.1"
                     /translation="MQEYPVKRGFTKDLDMRVSVELKNCFGVEPEKTAKGYRIRSGAL
                     KRLDVSVTPDGKSVIIDTESDLSAIDEVIIDTNKRFRKYLDAITGFSTKERVKRAKSV
                     PE"
     gene            12837..13562
                     /locus_tag="VFC43_06500"
     CDS             12837..13562
                     /locus_tag="VFC43_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome assembly chaperone family protein"
                     /protein_id="HZK30865.1"
                     /translation="MTTESPHDLKIFSKPTPDNAAVLMGFPGSGLVGTIALQYMVEQL
                     EFELIGTMTSKFFPPLAMMNKGVINDPVRIYTKNDIAAVVADIPIHPMICYEIANGLL
                     DWLEPFKPKEVLTIAGIITNEPEKRVFGVATTTDALKRIQEYTQVLPIGSISGIASSI
                     LTECKIRGIPAYGLLGETVNAPDPRSSAATIEVLNKMYNLGLDTKPLIDQAVEIEQSM
                     GKIAEEVQQSAEQSPKKDLPMYG"
     gene            13568..13966
                     /locus_tag="VFC43_06505"
     CDS             13568..13966
                     /locus_tag="VFC43_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF473 domain-containing protein"
                     /protein_id="HZK30866.1"
                     /translation="MRCAALTGISPELIKDLKKGNPRTIELQSTHNIVSIATITPGPD
                     THVFMTSVDHEDLGPGDAGICAYVLATSISMKRMVEFSHGSYFEERERTSARVQVKYC
                     ATSVVKQVFHEDNVGPTQVEVLKSSCYHAG"
     gene            complement(14206..15225)
                     /locus_tag="VFC43_06510"
     CDS             complement(14206..15225)
                     /locus_tag="VFC43_06510"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HZK30867.1"
                     /translation="MKSSFFIVCLFATTLILALVVPVAAAPSIDTINPSHAYNGGKVS
                     GIVISGSGFNVTTGGVMLRMSGERNIIATITNPTTTSLTCSFPLSGAEPGNWDVVVIN
                     RDRTENVLSEGFTILSTITLTSVSPNYAQTNNDSVKVTVAGTGLSDIQSLYLYNSEYD
                     NITANIDDTTSTKVIGTFDLTDVTIDSYDICVEDSYGGIKCGLNFDIVTDEVGSVDVE
                     SSPSGAKVYLDSDYVGTTPYTIEDVSLGSHKITVSKTGYVDYIKWVTVKVDSTSSVSA
                     NLDAVAASATTNVPTVATTKTPLKVTTVKVPTSWPTPATTQTSLDATLVLGAIALGVL
                     VLCRK"
     gene            15363..15437
                     /locus_tag="VFC43_06515"
     tRNA            15363..15437
                     /locus_tag="VFC43_06515"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15398..15400,aa:Arg,seq:tct)
     gene            15749..>16210
                     /locus_tag="VFC43_06520"
     CDS             15749..>16210
                     /locus_tag="VFC43_06520"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30868.1"
                     /translation="MAMLIFVLLFVPAAEAAPAVNGITPATGLNTSTVSLTNLNGTGF
                     ISGASVQLTPVNVIPVHRGSIQSGSGGALLNNPYDVFVSGNYAYVASFGSNALEIVNI
                     SNPASPFHAGSIVDGSGGALLDSPYSVFVSGNHAYVTSYDSNALEIVNISNP"
BASE COUNT         4214 a         4039 c         4096 g         3861 t
ORIGIN      
        1 ctattacagc cggagcagga agaaactctg gaagaagggc gaagagagcg gtcacttcca
       61 gaaggtagtc agcatcctta ctgattgcga tgaggactgc ctgctctatc tcgtggagca
      121 gcagggcgct gcatgccaca ccgggttcga atcgtgcttc taccgcacgc ttgagggcaa
      181 tgtagtaggg aaaaaggtgt ttgacccgga gaaagtgtac aggaaaaagg ggcactgaat
      241 taatctgttg agccggctgc acttttttgc accggcattt acttttttta gcgttgttat
      301 aaggcagaaa ggcagagaaa ttctcaatta caatcccgga taatataaaa agcatggatc
      361 cttctgccgc gattcgtgat ctgaaaggac tctccgaaca ggaagcactt gaacagctca
      421 caaagtccgg tttgaaccgg atgacatcac ggcacgagat cacattcttc ggaatcgcta
      481 aggaagagat aacagaaccc atgatcctct tgctccttgc agtcggggtt tgttatacga
      541 tcctgggaaa cctcaacgac gctctcacgc tctacgccat catcgcaaca cttgtccttg
      601 tggagatcgc caatgaatac agggcaaaaa aagccatctc gtcgcttgct ctgcttgccg
      661 aaccaaaaac cagggtagtt cgcgatggtt cgattaagga gattgaaaca gggtttgtag
      721 ttcccggaga tactctggtc attgtctccg gtaccaggat agcggcagac gggagagtct
      781 tgtttgccgt aagtctccag gttgatgaat cttcactgac cggggaatcc ttcccggtag
      841 aaaaggtaac gggtgacgag gtatcaggag gcacgctcgt tgtcgcagga gaagggatga
      901 tggaagctgt ttctaccggg gggtctaccc gtatcgggaa actcgctgcc cttgcgcagg
      961 aaataaggca gccgaagacc ccgctccagc tcgccatgaa ggcactggtg aaaacactgg
     1021 tgtttgttgc cctgttcttc tcgatcagta ttcacctcat cggattttta cagggacaac
     1081 cgctccgtga aatgttcctt atcggccttg cccttgcgtt tgccaccatc cccgaagaac
     1141 ttccgataat tatcacgatg aacctgggcc tcggcgctta tatgctctca aaggaaaact
     1201 ttcttgtaaa gaaattaaaa gcagctgaga ccctgggtaa cgcaacggta atagtcacag
     1261 acaagaccgg aacgctgacc gagagcacta tgcatgttgc gtcggtcttt cctgaaaata
     1321 ataccgatag tatcctgcgt tctgcccgtt ctgcaatgac ggatatttct gactcaatca
     1381 ccgatcgggc tgtcatcact gccgctgaaa tccaccacat ctccccggat ccaggcctgg
     1441 tcatacggga gcggtcgttc gagacaggaa aaaagacccg ggcacttatc cgtgaccaga
     1501 atgggagcca agtcatgtat ttgatcggtg cccccgagga gatacttgcc ggggtaaccg
     1561 gtgaaacaaa ggaggcagct gccgcacttg caaaagaaac tgaacggggc cgtcgggtca
     1621 ttgctgtcgc acaccgggca atcagttccg aagagaggga gattccgtta cccgcactgg
     1681 aacgggatct tatactcgac ggactgatag caatagaaga tcccccgcga cctggtgtca
     1741 gagaaacaat agaacgggca caccgtgccg gcatcaggat catcatggtg accggggacc
     1801 acccgcagac ggcaacggct attgccggcg aggtcgggat cccccatgac cgcgtacttg
     1861 aaggaaagga tattgacgca cttggagata ccgatctccg tactacgctc gcggaggttt
     1921 ccgtgtttgc ccgggctacc ccacagcaca agtaccggat cgtcaaaaac ctccaggaaa
     1981 aaggagaagt tgtggctgtt accggtgacg gggtaaacga ttcgcttgca ctgaaagccg
     2041 cagatatcgg gatcgcgatg gggatcaaag ggacggacgc cgcaaaagaa gctgccgaca
     2101 tcgtgattgc tgatgacaat ttcattacga tcggcaaagg tatttttgaa ggcagacgaa
     2161 tattcgataa tctctcaaaa ggggtaaagt actacctctc ggtaaaagtc gccctcatcg
     2221 cggtctttgc tgcatcgctc gcagccgcta tcccgtttcc cttctccccg gtccagatca
     2281 tcctgctcga actcttcatg gaccttggag cttctgcatc ctttgttgca gaaccgcccg
     2341 agccgtccat tgatcagcgc ccgccacacg atccgaaaat tcccttcctc aactccccga
     2401 tgctggcggg catagcactt ggcggggctg cactctgggt tgcggtgttt ataccttact
     2461 ggtcagccct gtatcttggt ataccgttgg cagcggccca gaccgctgcg ttctcctcgt
     2521 ggatgattgg gcatatcctg ctggcattct tctcgcgttc agcccatgat ccgctctacc
     2581 ggacaggagt gttttcgaac cgtgtgatgg tcctgtgggc aggaggagca tcagcatttc
     2641 tcgcactcct cctcactgtt cctgcaatcg gacagcgttt cggcataacg cccataagcg
     2701 ccgggacagt tcttgttatc gccggtttcg ccctgtcatg catggcaggg ttcgaactgg
     2761 caaaattttt taaaaaaagt gagaaaatga caacgggata acggggaagc accacaggac
     2821 aggaaaacag gagactgtca gcatatattg atgacctgta ccgggcagag attcgctgct
     2881 tcccgtgcac aacaacaaag atcatctggc acaattccct ctgcccggtt gcagctgaaa
     2941 cgatattctt cgacgatttc tgagaacgaa tcgcagggat tctggccaaa gatttccggg
     3001 cagatattcc agcacgcccc acagctgaca catccccccc ggtcgatact tacgttcatt
     3061 ttcccttaac ctgccggatc tggtataaag gttgccatgg ggaatcataa aggaaaccac
     3121 acgcgatagc ttacctcaca tcccttcgaa ccccttttaa accccgcggg gtaatatata
     3181 cccatcaacg atcttcaggt cgttgaacca gataaccggc gggagcggct ggatggcgct
     3241 cgaaccggta aacgagggac tgcgagcgct cgcatatcct gtacatcaag agaatagcat
     3301 agtataccac agcataccat aacggagtga taatatttta tgatattagt accagatacg
     3361 agcgtcctca tcgacggacg catcacatcg atgataaaag ccggtgaata caaaggcgca
     3421 acaataatcg tccccgaagc agttattgca gaactggaag cacaggcaaa taacgggcgt
     3481 gaaataggat tttccggctt gaacgaactg cagcagctct gcaggatggc tgaggagaag
     3541 acgatagaac tcaagttctc cggtatccgc ccctcgcttg agcaggtcaa gcttgcaagc
     3601 ggcggcgaga tcgacgcaat gatcaggaat atcgcaatcg agaattcggc acggttcatc
     3661 accagtgatt tcgtccaggc tgaagttgca cgggcaaaag ggttagacgt catctactta
     3721 aaagcccagg tgagcgactt tgtccccctc ggtatcgacc agttcttcga cgaacacacg
     3781 attgcagtgt accttaagga aagagtctgc ccgtcggcaa aaaagggcac gataaacgat
     3841 atgaaactgg tgaagatccg cgagcagccg accagcgaat acgagctccg ggcccttgca
     3901 caggaaatcc tcgagcgtgc gaaacgggat cctgacgggt tcatcgaagt tgagaagcgg
     3961 ggcatcaccg tcgtccagat cggttctatg cggatcgcca tcgcccgccg tccgttctct
     4021 gacggcatgg agatcaccgc tgtccgtccc atcgttgatg tgaccctgaa agactatgca
     4081 aaagccgacc tcataacaaa aaggatcacg agcgagaaac gggggttgat tatcgttggt
     4141 tctccgggtt caggcaaaac taccctcgca cagagcgtcg cgacctacct ttctgatagc
     4201 gggtatgtcg tcaagaccat ggaggcaccc cgggaactcc aggtaccgga ccagattacc
     4261 cagtacacga tgctcgatgg cagcatgtcc aacaccgctg atgtgctctt actggtcagg
     4321 cctgattatg tcatctttga cgaacttcgc aagaacgagg acttcaatgt ctttgccgat
     4381 atgcggctcg ccggtcttgg catgatcggt gttattcatg caaatggtgt gcaggacgca
     4441 gtccagcgtt tttccgaccg ggttgacttc tcggtcctct cacagatcgt aaacacgata
     4501 atctttgtga aacagggcgt gattaccaac atatacgatg tgggtttcac gatcaaggtc
     4561 cccgagggaa tggcaaatga aatgcatatt cgcccggtga ctattgtaaa cgatcacgag
     4621 acaggagaac tggtcattga tgtcttccgg tatgatggcg agaccatagt gatgcccatg
     4681 tctgcagtgg caaacgcagg aaaaaaagtc accccgcctg tacctgtcca gaatgtcggt
     4741 gtatcggtag cagcaacggg taacgtccgt tcaccggttc ccctgccgga attatcgcct
     4801 aagaaagaaa atgaagaacg tcccggctgg aaaatcatgg agaaagatat ccagcgtgaa
     4861 ctcgggcgtt ataccgaggg ttttgttgac gtccagatga tcagcgatac caaagccatc
     4921 gcttatattg atgacaagga cgtaccggct gcaatcggca agggcggcaa gaatgtgtct
     4981 gcaatcgtaa acaaaatcgg aatcgggatt gatatcaagc cccactctga atttgaccgt
     5041 atgcagatac agccgcaaaa aaccgatatg gagctcaaca tcggaggcgg cataaagata
     5101 cagacagata aaaaacagct cactatcgtt gccccggaac agagcggtaa gattgttgac
     5161 gtgtttgcag gcaaggagta cctgtttacc gcaaccgtga acgacaccgg agagatacac
     5221 ctctccaaga actcaagcat agcgcaggaa atgatccgtc gctaccagaa cagcgagatt
     5281 attaaattga gaccggtata acccggaata tttttttatt tttccaaaat acacgaatgt
     5341 gaaatggttt gaactcattt gatctgcaaa aatttgaaga agatgcacag gagcagtgga
     5401 atcatgcctt tgaatccaac ccgtcggacc tggaaaaatt ttacctgacc gtcgcctatc
     5461 catatccaag cggggcgatg catgtcgggc acggacggac atatatcgtt cccgatgtga
     5521 tcgcacggtt ccaccgcatg cagggtaaac aggtcctcta cccgatggcg tttcatgtca
     5581 ccggtgctcc ggtcataggt atttcaaaac ggatcgcaaa gaatgatccc aaaaccattc
     5641 acctgtaccg ggatctctac aaagtcccgc agaatgttct cgatgagttt accgaccctc
     5701 tcaccattgt ccgccatttc gccagcgagt accagagagt aatgactgca tgcgggatct
     5761 cgattgactg gcgccgcagg ttcaccactg tggacccgac ctacagcaaa ttcatcgagt
     5821 ggcagtggaa gtacctcttt gaagcaggtc atgtcataaa aggtgctcac cctgtccgct
     5881 actgtacggt ggacgacaac cctgtcggag accacgacct gctggaaggg gacaaagcag
     5941 aggtaataaa gttcacgctc atcatgttcc ggtatggcga tgcatttatc cctaccgcga
     6001 cacttcgtcc cgaaaccata tacggggtta ccaatctctg ggcaaaccct cacgtaacct
     6061 acgtaagggc tcttgtagat ggaatcccgt ggatcatatc ccaggaggca gcagagaagc
     6121 ttgctttgca ggaccacact gttgaagtaa cagaaaacat tgccgggcag gacctgatcg
     6181 acaagaccgt tgatcacccg ctgtgtggca cagtcccggt gctcccggca gactttgtgg
     6241 accctgacat ggcaacagga cttgtaatga gtgtcccggc acatgcaccc tttgactaca
     6301 ttgcactccg tgacctccag caggcaggaa aatataccac tatcagaccg gtgcccttaa
     6361 tcagggttga agggtacggc gatattcccg caaaggacgc ggtcgagcgg gcgggtatct
     6421 cccaccagat ggacagccgg atggatgcac tcacgcagga gatctactct gcggagtttt
     6481 ccaaagggaa actctatgaa aaatatggcg gtaaacccgt gcgttttgcc cgcgatgaag
     6541 ttgccctgct catgatcgac cagtacgaat cggagatcat gtacgagttt gacacacgcc
     6601 cggttgtctg ccgttgcggg aacagggtaa aagtcaagat cctgcatgac cagtggttcc
     6661 tgaagtacag tgatcccgca tggaagaaac aggtgagcga ccacctggga gatatggcgc
     6721 tggtccctcc tgaggtccgg cttgagttcg accgaaccgt ggactggctc aaagactggg
     6781 catgtacaag aagagtcggt cttggcacca ggttcccgtg ggatacgaca caactcatag
     6841 aaccgctctc ggattcgact atctatatgg catattatac aatagcccac aagatccgtg
     6901 aaatcgatcc aaaactcctc aaacctgaag tctttgacta catcttcctc gggaaacagt
     6961 ctcctgaact tcctgagaag aagaaactcg atgccatgag aaaagagttc ctgtactggt
     7021 acccgtataa tttccggttt tccgcaaaag acctgatctc caatcacctg acgttccagc
     7081 tcttccacca tgtcacgata tttccaaaag acaaactccc gcagggtatg gttgtttttg
     7141 gcatgggact cctcaacggt gccaagatgt cttcgtcgaa aggaaatgtg ttcctgcttg
     7201 aggacgctgt ttctgatttt ggcgccgata ctgtgcgcat gttcctgatg ggcagcgccg
     7261 aaccctggca ggactttgac tggagaaacg agctggtcat ttccacaaaa aagcagattg
     7321 aacgcttctt caacacggtt ggtgagtcca aagatgcgga tggcgagccg catgatatcg
     7381 atcactggat aatcagcagg ctgcagtcgc acatcgagcg gacaactgaa gcacttgcca
     7441 acttccagac ccgccaggca ttgcaggagg cctatttcgg gatcgaaacc gatctgaaat
     7501 ggtaccgccg ccgtcttttt gaaaactgtg atgggagcag ggagttacat acactctgct
     7561 cggtatgggt ccggctcctt gccccgttta tcccgttcac ctgcgaacat ctatggaagg
     7621 aaatcgccgg tgaaggactc gtatctttcg ctccctggcc ggttccggac aagaaactgg
     7681 tcaatacccg gattgagctg gccgaggagc tcctgtcccg aacagtcgag gatatcgaat
     7741 cgatcatgaa acttatccag attactccaa aatctgttac catatctgtc gcacctgcat
     7801 ggaagcagga aattttcctg cacattgcac gtgcagagga caggacaaac gtgatcaggg
     7861 atatcatgaa agatgatgca atgcgcaagc ggggcaggga ggcaactgat gcagcaaagc
     7921 agtgcacaac ccttattcac cgcctgccac cgcatgtggt cggtcacctt gcgaaagaac
     7981 cggtcaatga actggctgtc ttccaggcgg caaaaagctt ccttgaaaaa gaatttggcg
     8041 tgccggtcca tgttacccag gccgagtcaa gtgaacatgc aaaagctgct accgcgttgc
     8101 catttaaacc ggcgattgtt attgaataat acctttttta cctgactggt cgtcacaaag
     8161 aagaatcaac gacttaacta cggtaaattt taaaaaaatt atttcctgat tgcgttccgg
     8221 accttgaaga tgacgtctgc tgacagaacc tgcatcttgt gagcaggttc cgcatggtcg
     8281 ataaattttc tcatcagttc gtggtctgtt gatccatgag cttcaaacgg gcagtcaatc
     8341 ccaatatctt tgcacataaa tgatggcatt tgtataaaat ctccttttct ttcctagact
     8401 tgaccacatg cgaatatgaa gaaatgcaac gcagtgctgc atggcaccga tgtgtttttg
     8461 gagataagac acatgaaaaa tgaacactct ttactgcaca ccatttttct tccacaaaca
     8521 agttataccg aagaattata ctctctttaa aggatacaac catgaacact cgattccgga
     8581 ttaatctcgt tataacagga ttcctgcttg cgatctgcat tataggcacg gttacggcag
     8641 caagtgtggc gcatacctgg caagagcagc ctgcattcga tggttcattt tccgggctga
     8701 tgttttcttc ggacgggtca atgatatttg ccgggggcag ccagttgttg gtccgttcat
     8761 ggaatggaga catccactgg ggggggcgaa caggcactgt tgctgccatg aactcagatg
     8821 ggacccgggt tatttcagca cttgattcaa gtgtccgggt gctcgacaaa aatggtgaag
     8881 aggtctggac cagaattttg ggtagttccc cgatacgtgc ggttgccgtc tcaagggacg
     8941 gttcacttat tcttggcgcg aatgatgagg ggtatatcca ggcatgggat gtaaatggga
     9001 ccaaaggggg actgaacaaa accgaccggg taaaaaagat agcaatttct ccctctcaga
     9061 ctctgatcgt tgctgctaca gaagcaggtc tgaaattttt ttcacccaaa atggagcaga
     9121 tctgggctga cgataaagaa ggaacccttg attcgttcat cgcattctct gcagacagca
     9181 gtaccgtgat cacttcagga gataaacggg tatggtcgca tacaggcacg ggtaaactta
     9241 actggatgag agaggtgaca caaaccggca tcacggatat ggcctgctca gatgatttaa
     9301 ctgctattgt gcttggcaga aaggaggggg atgtgctggt cctcgatgac acgggacagg
     9361 aacgatggaa atatcctgca ggttcgtgga tcgagggagt aggagtctct tcagatggct
     9421 ccataattgc cgcaggcgcc ctggacggta cactctatat cctggatcgg aatggaaacc
     9481 tgctgttaaa aacaacaacc ggcacgctca tccagcagga atcggttctg gtcagccggg
     9541 atggtaaacg catcatggtc gctgatgaag gaaccctgta tggttttgat ctcctcggat
     9601 tacctgaagt aacgccagcg gtaactcagt ctcccacacc ggagatccct tctacctgtc
     9661 ctacatgtcc tgcctgtccg gcacccgtaa caactgcatc tcctgcaagg acaactgtcc
     9721 cttcaacaac aggcactccg gcggcaccgc ttaacccgta ccttggattc attgcatgtg
     9781 ccggtggagc gttgatggta ttgagacgta agacctgatg caaagtcttc ctccctgtag
     9841 aggaaaaaag gatacagttt tttttagaaa aattttaacc ggacaccgcc tgccctgaaa
     9901 taaccaaagg gcatgtgtac cctctcacca tagaccgttt taagaaggtt aataaaccgg
     9961 tcgtcctcgc caatcaggta ttcgaatttc ccgccagacg attcaataat tattttgaat
    10021 tctgtatatt ccatttcgcc ggaatcattt aatttcaggg taattgcggt aatttgtgta
    10081 ttttcaatgg caaaatttcc cgctgtttcg gcaagggact gatcaggagg catcgtctcg
    10141 tagcggagtg caaaacggaa ctgtgcagcc atctggtctt tcacgatcgc aaaaaacccc
    10201 ttgccttcgg cctttgcctt tgcctgtgct tccatgatag cagcgttgag cagggactgt
    10261 gtcatctgtg caagaatcat acgccggttt gtgacaacga tggtgtacgt atcccagctt
    10321 gcgccgaaca ttttcatttt ccgggcattg gcaatcaccc ccatcacttc ttcaccggtt
    10381 gcaaccgcgc ttccttgagc accttgtgta ggcacgaagg gtgccgtggc agcaggtggg
    10441 attgtaccgg atgacgcacc agctccaaca gggattccgc atatcccaca gaatttttca
    10501 attcccccaa gaggactgcc acaggactta cataccggag gagtaacaga tgacgctgca
    10561 atatcaccag tggattgccg ggtttgtggc ggagccaccg gataatcctc aactaccttt
    10621 gccaggcatt ttttgcagaa cttatctccc gggttgatta tatagccgca agagcggcat
    10681 aaccgtacac cctggtgttg tgcggggata gtttctgacc catgatcagg taacggggag
    10741 ggtggtgatg atggtggaga gacaggggga accggtgctt tcaacggtgc tggagattcc
    10801 aaagtcgaat ccaccacttt ggcgaggcat ttaccgcaga atttatctcc ggggtttatt
    10861 acatttccac aggaccggca taaccgcatc ccggcctgct gcgttgagga ggtcccggcc
    10921 ccaaaatcag gtatcacggg gggtggtggg ggaggggaaa caggaggaac cgccacctta
    10981 accggtaccg gcgattccaa agtcgaatcc accacttttg caagacattt gccgcagaat
    11041 ttatcaccgg ggtttattac atatccacag gaccggcaca cccgtatccc tgcctgttgt
    11101 gggggttgcg gtgcgggggg aggaatgtat tgggactggg gagacggtga tgcagttatc
    11161 accgcaggga ctgtcaatgg agcgggaatg ccatctcctt ttacagggct cccgcatcct
    11221 ccgcagaacc gcgtaccggg attgaggggc atgccgcaca atgtgcagga gagcgatggg
    11281 gaaggtacag gtgccgggga ggggggaggt gataccggag gtgcagccgg aatcacctgc
    11341 ggcctgattt caccttccct tgccccacag acaccgcaga tcatttcatc ctgtgaaagc
    11401 gggttgctgc aggaaccaca ggtccgcttg acctcaggca ccgctgcagg gggagggttg
    11461 gatggcgatt cttccagttg tgatgcgccg cagttccggc agaattttgc ggtgggcgcg
    11521 actcgtttac cgcattcggg acagtatgac atgatatcac gttacatgaa gttgaacccg
    11581 ctttcttatg agcattgctt caggaaaaaa ccatgtgggg aaaatccgtt acccgtatac
    11641 ctcccatggc ggtttttgtt gtggcaaccg gactaaatgt ccctcatgaa gggtcttata
    11701 tatccttttt attgtcatag gggacttgtg cggtctgatg atatgtgtag ttcgtaaacc
    11761 agagagggaa aattggggag agaagattac aggttatttt ttgcaacctg cggtgcatat
    11821 tttttaacca ttgcgagaac cggcagaaaa ttttcttctg aacaatagag aacaacagga
    11881 cctatcaggt atctggaacg aaagacaatg ctctgtgttc tcgggtaaac agagatgtac
    11941 cgcacatcct cccacgggag gactgtgttt tcctgtgata ttgcactaag tcctgcaccg
    12001 gttcctgcca gtgatccaag catcgctgaa cctcctgctg aggcacggtt gagcgcgcgg
    12061 gtcgtactcc ccgcattatg agcaacacct ttgttactga taagaaattc agtatcaagc
    12121 ccgccgccgg ttaccagctg aagcaccagc atgataagga tcatcagcac ggagagcccg
    12181 gcactgatca caaaaaacaa cacgagcatc tcaacgcttc cggtaacaag ggaaagaaaa
    12241 ccacccatca ctatcgcaat tcccaggggg atgagaatac actgtaaaac gagatatggg
    12301 tttgttatca ggggaatttt tcggttccat ataacgagtt cgccggaagt tcctgccgtt
    12361 aatttggtat ctcctgatcc gtcattttcg ggtgaggagg caagcgccgt cccgcaggcg
    12421 gtacaaaacc gttgaggttc cggcacctcc gttttgcatg tttcacagag cgtcatgagg
    12481 cacaggttat tcaggaacgc tctttgcccg cttcacgcgt tcctttgtgg aaaaaccagt
    12541 aatggcatcc aggtatttcc ggaacctttt attcgtgtca atgatcactt catcaatggc
    12601 gctcaggtca gattcagtgt caataatcac tgatttgcca tcaggagtga cagatacatc
    12661 gagacgttta agcgcaccgg accgtatacg gtagcctttg gctgtttttt ccggttcaac
    12721 accgaaacag ttcttcagtt caacggaaac ccgcatatca agatccttgg taaatcctct
    12781 tttgacagga tactcttgca taatactttt ttatgtggtg ggtctatgtt aatctaatga
    12841 cgaccgaatc tccccatgat ctaaagattt tttcaaaacc aactcctgac aatgcagcgg
    12901 tgctgatggg atttccaggc agcgggctcg tcgggactat cgcccttcag tatatggtcg
    12961 aacaactcga attcgagctg atcggcacca tgacatccaa gttttttcct cccctcgcca
    13021 tgatgaacaa aggtgtgatc aacgacccgg tcaggatcta tacaaaaaac gatatagccg
    13081 cggtcgtggc tgatataccc atccacccga tgatctgtta cgagatcgca aacggccttc
    13141 tcgactggct ggagccgttc aaaccaaaag aggtgctgac gatcgcaggg atcatcacca
    13201 acgagccgga aaaacgggtg ttcggtgttg caacgacaac tgatgcgctc aagaggatac
    13261 aggaatacac ccaggttctg cctataggaa gcatctcagg aattgcatcc agtatcctca
    13321 cggaatgtaa aatccgcgga atacccgcgt acgggctcct tggtgagacc gtaaatgcac
    13381 cggatccacg atcttcggca gccacaatcg aggtgctcaa taaaatgtat aacctcggcc
    13441 tggacacaaa gccgctcatt gatcaggcag ttgaaataga gcagagcatg ggtaaaattg
    13501 ccgaagaggt gcagcagtct gccgaacaat ccccgaaaaa agatcttcca atgtacgggt
    13561 gagaaagatg agatgtgctg cattaaccgg gatttcgccc gagctgatca aggacttaaa
    13621 aaagggaaac ccccgaacca ttgaactgca gagtacgcat aacatcgtga gtattgccac
    13681 gattacgccg ggtcctgaca ctcatgtttt tatgacatct gtcgaccatg aagacctggg
    13741 cccgggggat gcgggaatat gtgcctatgt acttgccaca tcaatctcaa tgaaaaggat
    13801 ggtggagttt tcgcatggct cgtatttcga ggaacgggag cggacatcgg caagagttca
    13861 ggtgaagtac tgtgcaactt cagtcgtcaa gcaggtcttc cacgaagata atgtcggacc
    13921 aacccaggtc gaagtactga aatcatcctg ttatcatgcg ggttgaagag tatctttttt
    13981 tatccagaca gattgacttc ggtctatggg caaagttcct ggttcaggaa aaattgagga
    14041 tcctttttgg tattccggat tccctctttt ctgatatgaa tttcatctga ataagaaaaa
    14101 gcatcttttc cggatatccg tggattaatg attattgagc aaatctggct gttcctatac
    14161 atttcccgaa ctaaaagaga gtgaaagata tgttaaaaaa tattattatt tcctgcagag
    14221 aaccaaaacc ccgagtgcaa ttgcccccag aaccagcgtg gcatccagcg aagtctgagt
    14281 ggttgcaggc gtaggccacg atgttggcac ctttaccgtg gtgaccttca ggggggtttt
    14341 tgtcgtggca accgttggta cgtttgtggt agcagaagct gcaacagcat cgaggtttgc
    14401 actgacactg ctcgttgagt caactttgac agtcacccat ttgatatagt caacatagcc
    14461 ggtttttgaa accgtgatct tgtgggatcc tagtgagaca tcttctatgg tatagggagt
    14521 tgttccgacg tagtctgagt caaggtagac cttggcaccg gaaggacttg attcgacatc
    14581 gacactgccg acttcatcgg taacgatgtc aaaattcaaa ccgcatttaa tcccaccata
    14641 tgaatcctcg acacagatat cataggagtc gattgtcacg tcagtcagat cgaatgttcc
    14701 tatgactttt gttgacgtcg tatcgtcaat attcgccgta atattgtcat attctgaatt
    14761 atacaaatac aggctttgaa tgtcagacaa tccggtaccg gcgacagtta ccttgacact
    14821 atcattgttg gtctgtgcat agtttggtga cacggaggtc agcgtaatgg tacttaaaat
    14881 cgtaaatcct tctgacaaca cattttccgt tctgtccctg ttgataacaa cgacgtccca
    14941 gttaccgggt tctgcccccg aaaggggaaa cgaacatgtg agagaagtag ttgtcgggtt
    15001 ggttatggtg gcaataatat ttctttcacc ggacattctc agcatgactc ctcctgtggt
    15061 gacattgaat ccggacccgg aaataacaat ccccgatacc ttccccccat tgtaggcgtg
    15121 cgacggattg atagtgtcaa tacttggcgc agctgcgaca ggtactacca gcgctaaaat
    15181 gagggttgtc gcaaacaggc atactataaa aaatgaactt ttcattctgg tcatcccgta
    15241 cacaatgaat aaacaaacga gaaatcgcca tatatatttt gttcatcttt ccggttcgtg
    15301 atatcaaccc cccatgacac agattaatta tcacaaaaca ccaaacttat tggcacttag
    15361 ttgggcttgt ggcttagctt ggacatagcg ccgggcttct aacccggatg tcgggggttc
    15421 gaatcccccc aggcccgttg taagaacaat tgagaatcct ttgcaaagga aaaacccaga
    15481 tcctccatca cttttttact tctccgttca tcagacatgc acgatgattc aattttacag
    15541 caggataatt ggcggtttca ggaattcaaa ccaaaacaag gtcaggagtg gaaaatagtg
    15601 caaaaattac ccccaatcat gacaatatat tgctcttgac aaaaagacat aaattaatcc
    15661 aatgtccgac tataactgcc aatgttccag cagggatcgt ccaggttcgt atcgtttctc
    15721 cctatcccga attacatctt ttttcgtgat ggcgatgctg atattcgtgt tgctgttcgt
    15781 tcctgctgct gaagcagcac cggcagtaaa cggcatcaca cctgcaacgg ggctcaatac
    15841 gagcaccgtc tcgcttacga acctgaatgg cacaggtttt atatccggtg catctgtgca
    15901 gctgacaccg gttaatgtta tcccggttca caggggcagc atacaaagtg gcagtggtgg
    15961 tgcactcctg aataacccct atgacgtgtt cgtctcaggc aactatgcat atgtcgcaag
    16021 ctttggcagc aatgctcttg aaatcgtcaa catctcgaat cctgccagtc catttcatgc
    16081 ggggagcatt gtcgacggta gcgggggagc gctcctggac agcccctata gcgtattcgt
    16141 ttccggtaac catgcatatg tcacaagtta tgacagcaat gctcttgaaa tcgtcaatat
    16201 ctcgaatcct
//
LOCUS       DASRLV010000166         7210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_790514,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000166 DASRLV010000000
VERSION     DASRLV010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7210
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790514"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VFC43_06525"
     CDS             complement(<1..297)
                     /locus_tag="VFC43_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HZK30869.1"
                     /translation="MAQNIGLNVPAPKQECQDVNCPFHGTLPVRGQVITGKVVSDRMM
                     GTVVVARNYLHYIRKYKRYEKRSSKLHAHSTPCLHAKVGDMVKIAECRPLSKSTT"
     gene            complement(297..581)
                     /locus_tag="VFC43_06530"
     CDS             complement(297..581)
                     /locus_tag="VFC43_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0001682 - tRNA 5'-leader removal [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein subunit"
                     /protein_id="HZK30870.1"
                     /translation="MISPQNVLRHELIGLDILVSVAKNPLHKGLGGCIIDKTKNLLII
                     ETPRGIKRIPKLHSRFQLHLPGSDIVEIDGSVMVLAPEKRISLHEKKRIK"
     gene            complement(578..781)
                     /gene="rpmC"
                     /locus_tag="VFC43_06535"
     CDS             complement(578..781)
                     /gene="rpmC"
                     /locus_tag="VFC43_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HZK30871.1"
                     /translation="MAILRAQEIQQLSAVELQEQMEKLRMELVQHYGKVSAGGATENP
                     GHISEIRRTIARLMTEQNRRRNV"
     gene            complement(781..1479)
                     /locus_tag="VFC43_06540"
     CDS             complement(781..1479)
                     /locus_tag="VFC43_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HZK30872.1"
                     /translation="MAVEQKFISEGVRKARVEKYLSKELKRAGYGGMDIARTPLGTQV
                     TIFAEKPGIVIGKGGKLVHQLTLDLAQNYGVESPQIEVQQVTNPSFNAQIMAERLANA
                     LERGWYFRKAGSSILRRVMDSGALGCEVVIAGKLTGARARTQKFTEGYIKHCGEPSNT
                     IVEKGYAVAIKKLGVIGVQVKLVPAGAKLPDHFSLIEQEKKRLPPKITVTDIADIDSD
                     EPALPDEIPFREDQ"
     gene            complement(1479..1943)
                     /locus_tag="VFC43_06545"
     CDS             complement(1479..1943)
                     /locus_tag="VFC43_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HZK30873.1"
                     /translation="MARIDYSQKITGDNIAKAKANELNMSPKHSIEIATFIRHQRVND
                     AIAYLNDVVSLKKAIPFRRFNRNVAHKRGLPGNWDAGRYPVKASKAYIRVLESVKKNA
                     EYIGLDADNLEIVHVSANRGRAQKSFFPRAMGRATPKVRESVNLEVVVREVV"
     gene            complement(1949..2368)
                     /locus_tag="VFC43_06550"
     CDS             complement(1949..2368)
                     /locus_tag="VFC43_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HZK30874.1"
                     /translation="MAAPKKTQKRMPRRREEFTYRGYRIDELKAMGISELLPLMPARA
                     RRKIVRGFSRGEEILLTKVRKGDEKIRTHLREMIVMPEMVGKSIEIYNGKEFMKVEFQ
                     PESVFHYLGEFALTRRKVAHGSAGIGATRGSKYVPLK"
     gene            complement(2374..3090)
                     /locus_tag="VFC43_06555"
     CDS             complement(2374..3090)
                     /locus_tag="VFC43_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HZK30875.1"
                     /translation="MGHRITTQSRGKGGPTYRAPSHRYKAELKHIGDDTQKIMGNVID
                     IEHDPARNAPIALVKLDDGTKVYMLVTEGLGIGEVVSWGSDVEVKNGNTLTLQYIPTG
                     TYICNIEARPNDGGKFVRASGVQAVVIDKIEDRVGIRMPSGKTKWFNHRCRATIGIVA
                     GGGRVDKPFVKAGNKYHKMQNTASNWPRVRGVAMNVIDHPFGGGGHQHTGRPKTIARG
                     TSPGRTVGHVAARRTGKSRK"
     gene            complement(3099..3347)
                     /locus_tag="VFC43_06560"
     CDS             complement(3099..3347)
                     /locus_tag="VFC43_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HZK30876.1"
                     /translation="MALKYPFVTEKAMVLLENQSKLQFLVGRQATKSQIKREIEKAFG
                     QNVKSVRTLMTMHGEKKAIVSFENEKAAEEILSRLGIM"
     gene            complement(3348..4094)
                     /gene="rpl4p"
                     /locus_tag="VFC43_06565"
     CDS             complement(3348..4094)
                     /gene="rpl4p"
                     /locus_tag="VFC43_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HZK30877.1"
                     /translation="MKAQVKSLDGGIARDIELPELFSEEYRPDLIKKAVMALQSTRRQ
                     PHGTNPYAGICSSAVGWGSGRGSSHVPRIKNGSRAAKVPQAKGGREAHPPKVAKILIK
                     EINQKEKQKAFRSAVAASISSDLIRGRGHMFDGVVPVVFEDKFETLTRTQDVITALTH
                     AGVYHDIERAKESKKVRAGRGKMRGRRFKQRKSLLIVTAAQPLRAARNLSGVDVVTVD
                     QLNVEHLAPGMLAGRLTIWTEGALVRLEGR"
     gene            complement(4101..5114)
                     /locus_tag="VFC43_06570"
     CDS             complement(4101..5114)
                     /locus_tag="VFC43_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HZK30878.1"
                     /translation="MPKINRPRRGSLAFSPRRRAKSPIPKYQSWPLYNGVPILQGFAG
                     YKVGMTHVIMVDDHKNSPTEGKEIMVPVTVVEIPSMKVAAIRAYSKDTYGKHALTEVW
                     ADQLDTVLGRRITLPKNYDKGAALKKFSDAVASGIVSEIYAVSYTQPAILSGVPKKVP
                     DLMEIKVGGGDLKKQWEFAQEILGKEVSLNNVIQTGAYADITAITTGKGSNGAVKRWG
                     IALRKRKHSVGGKERHLGTLGPWNPHHVRWQVPQMGQLGYQQRTEFNKRILRTSDNAS
                     EITPAGGFLHYGVLKSPYILVKGSIPGPVKRLIRIRPAMRLGEHVVRMPTIQFVSVQS
                     KQG"
     gene            5333..5482
                     /locus_tag="VFC43_06575"
     CDS             5333..5482
                     /locus_tag="VFC43_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30879.1"
                     /translation="MDDNPHPGADVHIHQRPCLAKEDPDNNASICTLTGQWNGLVCST
                     STHAK"
     gene            complement(5802..6206)
                     /locus_tag="VFC43_06580"
     CDS             complement(5802..6206)
                     /locus_tag="VFC43_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfx family DNA-binding protein"
                     /protein_id="HZK30880.1"
                     /translation="MKDGLLTERQMEVLRYRKQGLTQQQIADIISTSKANVCTIEKSA
                     MENIRRAKETLEFLYTLDATHLCTIPSGTDLFDVPSIIFGEAEKINIKVSYDTISLIN
                     RLREIRPQCCKARCVCEDILVYITDLGELYFG"
     gene            6411..>7210
                     /locus_tag="VFC43_06585"
     CDS             6411..>7210
                     /locus_tag="VFC43_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420-dependent methylenetetrahydromethanopterin
                     dehydrogenase"
                     /protein_id="HZK30881.1"
                     /translation="MVVKVGVAKLGNIASGVMAELLLDERADREDMQTFMATSGTKLE
                     PADVDRVVSNLKAYKPDFCIVVSPNGVLPGPTGAREQLAAAGIPVIIITDDVTTKKEW
                     EGVKASKFGYIIMKADSMIGARREFLDPVEMSDYNGNLVKVLALTGAFRKLQLAVDKV
                     IDQVKAGKSGDALELPKIIMTTDKAVDGEFTNPFALAKARAAHEIAMAVAGQNVKGCF
                     MTKEWEKYIPIVASAHEMMRVAAVLCDEAREIEKAGDGVLRKPHKKDGT"
BASE COUNT         1678 a         1852 c         1657 g         2023 t
ORIGIN      
        1 tgttgtgctc tttgaaagcg gtctgcattc agcgatctta accatgtcgc caacttttgc
       61 atgaaggcag ggtgtgctat gagcatgaag cttcgaactg cgtttctcat aacgcttgta
      121 ttttcggatg tagtgcaggt agttacgtgc aactacaacg gttcccatca ttcgatcgct
      181 cacgactttg ccggtgatca cctggccgcg caccggtaaa gtgccgtgaa acggacaatt
      241 aacgtcctga cattcctgtt ttggagccgg aacgttcaat ccaatgtttt gtgccattat
      301 tttatcctct tcttttcgtg caggctgatt ctcttttcag gagccagaac catcacggat
      361 ccatctatct caacaatatc gctgcctgga aggtgcagct gaaatctgct gtgcaatttg
      421 ggtatgcgtt taattccccg aggggtttca attatcagca gattttttgt tttatcgatg
      481 atgcatccgc caagcccctt gtgcaggggg tttttggcaa ctgataccag aatgtccagc
      541 ccgattagtt catggcggag gacattctgg ggagagatca tacgtttctc ctgcgattct
      601 gctcagtcat caacctggcg attgttcgcc tgatttcact tatatgaccg ggattctctg
      661 tggcgccacc agcgctgact ttgccataat gctggacgag ctccatacgg agtttctcca
      721 tttgctcctg caattcaact gcggagagct gctggatttc ctgcgcacgt aaaattgcca
      781 ttactgatcc tccctgaagg gtatttcatc gggaagggcg ggttcatccg agtcaatatc
      841 agcaatgtca gtgactgtta ttttcggtgg taaccgtttc ttttcctgtt cgatcaggga
      901 aaagtgatcc ggtaatttcg caccagcggg aaccagtttt acctgaacac ctatgactcc
      961 cagtttcttg atcgcaactg cgtagccttt ctcaacaatc gtgttgctcg gttcaccgca
     1021 atgcttgatg tatccctcag taaacttctg ggttctggct cgtgcaccgg ttaattttcc
     1081 ggcgataaca acctcacacc ccaatgcacc ggaatccata acgcgacgaa gaatgctcga
     1141 tcccgcttta cggaagtacc agcccctctc aagggcgtta gcgagtcgtt ctgccatgat
     1201 ctgggcatta aaactggggt ttgtgacctg ctgaacttca atctgaggag attcaactcc
     1261 gtagttctgc gccagatcca gagtcagctg atgaacgagt tttccacctt tcccaatgac
     1321 tataccgggc ttttctgcaa agatagtgac ctgcgtaccc agcggtgtcc gggctatgtc
     1381 catcccaccg taacccgccc gttttaattc ctttgacagg tacttttcaa cacgggcttt
     1441 ccgaacccct tctgagataa acttctgctc tactgccatt acaccacctc gcggacaacg
     1501 acttcgagat tcacggattc gcggactttc ggagttgccc gtcccattgc acgggggaag
     1561 aatgatttct gcgcccgccc tcggttcgcc gagacgtgga cgatctcaag gttatcggca
     1621 tcaagaccta tgtattcggc atttttcttt accgattcca atacccggat atatgctttt
     1681 gatgctttca ccgggtacct gccggcatcc cagtttcctg gaagaccgcg tttatgagct
     1741 acattccggt tgaaacgccg gaacggtatc gctttcttta aactgacaac atcgttcagg
     1801 tatgcaattg catcgttcac gcgctgatgc ctgatgaacg tggcaatctc gatggaatgc
     1861 tttggcgaca tattaagttc gttggctttc gcttttgcga tattgtcacc ggtaattttt
     1921 tgtgagtaat caattcttgc catattcatc acttcagcgg aacgtacttg cttcctcggg
     1981 ttgcaccgat accggcactt ccatgagcga ccttccgtct tgtcagagca aactcaccaa
     2041 gatagtggaa cacggactcc ggctgaaatt caactttcat gaattccttg ccgttataaa
     2101 tctctatcga ttttccaacc atctcaggca ttacaatcat ttcccggagg tgcgttctta
     2161 tcttctcgtc acctttacgg acttttgtaa gtaatatctc ttcaccacgg gagaaaccac
     2221 gtactatttt gcggcgtgcg cgggctggca tcagcgggag gagttcgctt atccccatcg
     2281 ccttcagctc atcaatcctg taaccccggt aagtgaactc ttcgcgcctt ctcggcattc
     2341 ttttctgagt cttttttggt gctgccatac ccttcacttc ctgctcttac ctgtcctgcg
     2401 tgcagctaca tgtcccacag ttctgccggg agatgttcct cgtgcaattg tcttcggacg
     2461 acctgtatgc tggtgtccgc cacccccgaa tgggtgatca ataacgttca tagcgacacc
     2521 tctgacgcgt ggccagtttg aagccgtgtt ctgcatcttg tggtacttgt ttcctgcttt
     2581 cacaaacggc ttgtcgacac gaccgccacc tgccacaatt ccgatggttg cgcggcaacg
     2641 gtggttgaac catttggtct ttccactggg cattctgatc cctacgcggt cttctatctt
     2701 gtcaataaca accgcctgaa caccagaggc acggacgaat ttaccgccgt cattgggacg
     2761 agcttcgata ttacagatgt acgtcccggt ggggatatac tgaagagtga gcgtattgcc
     2821 attcttcact tcaacatcag aaccccagct gacaacttca ccaataccaa gaccttcagt
     2881 gaccagcatg taaactttgg ttccatcatc cagtttgacg agcgcaatcg gagcattgcg
     2941 tgccggatca tgttcgatat cgatgacatt tcccataatt ttttgagtgt catcaccgat
     3001 atgcttcaat tcagctttat accggtggga tggtgctcga taggtcgggc ctcccttacc
     3061 ccggctttgt gttgtgatac gatgtcccat gttcccacct acataatacc gagccgactg
     3121 agaatttcct cggcggcttt ttcgttctca aagctcacta ttgctttctt ttcaccgtgc
     3181 atggtcatga gcgtacgaac gcttttcacg ttctgtccaa acgctttttc gatttcgcgt
     3241 ttgatctggc tcttggttgc ctgacgacct acaaggaact ggagtttgct ctggttctct
     3301 aacaggacca ttgccttttc cgtaacaaac gggtatttca gagccattta ccttccctcc
     3361 aatcgcacaa gtgcgccttc tgtccagatt gtcagacgtc cggccaacat tcctggtgca
     3421 aggtgctcta cgttcagctg gtcaacagtt actacatcga ccccggatag gttgcgtgca
     3481 gcacgcagcg gttgtgcggc tgtgacaatg agcaggcttt ttctttgttt gaacctgcga
     3541 ccgcgcatct tgcctctccc ggcgcgaact ttcttgcttt cctttgcccg ctcgatatca
     3601 tggtagacac ctgcgtgggt gagcgctgtt atgacatcct gagtccgggt gagtgtctca
     3661 aacttgtctt caaagacaac cggaaccact ccatcaaaca tatgacctcg accgcggata
     3721 agatctgaag agatacttgc agcaaccgct gatcggaatg ccttctgttt ttctttctgg
     3781 ttgatctctt tgatcaaaat tttggcaacc ttcggggggt gtgcctcacg gcctcctttg
     3841 gcctgcggaa cttttgcagc acgtgaacca tttttgatac gtggtacatg tgatgagccg
     3901 cggccacttc cccaccctac tgctgatgag cagattccgg cataggggtt cgtcccatgg
     3961 ggttgtctcc ttgtgctctg aagtgccatg acggcttttt tgatcagatc cgggcggtac
     4021 tcctcggaaa agagttccgg aagttcaata tctctcgcaa ttccgccatc aagtgattta
     4081 acctgtgctt tcatctctga tcatccctgt ttgctctgga cgctcacaaa ctggatcgtc
     4141 ggcatcctca cgacatgttc accaaggcgc attgccggac gaatacggat aagcctcttg
     4201 acaggacccg ggatcgatcc cttgacgagg atatacgggc tcttgagtac accatagtgc
     4261 aggaaacctc cggcaggtgt gatctcactg gcattatcac ttgtccttag aatacgctta
     4321 ttaaactccg tacgttgctg gtatccaagc tgtcccatct ggggaacctg ccagcgtaca
     4381 tgatggggat tccagggacc gagtgtgcca agatggcgtt ccttccctcc cacggagtgt
     4441 ttacgtttac gaagggcaat accccatcgt ttgacggccc cattcgatcc cttgccagtc
     4501 gtaatcgcag taatatctgc gtatgctccg gtctggatga cattgttcag cgaaacctct
     4561 ttaccgagaa tttcctgtgc aaactcccac tgctttttga ggtctccgcc cccgaccttg
     4621 atttccataa ggtcagggac tttctttggg acacccgata atattgcggg ttgtgtatat
     4681 gacacggcat agatctcgct tacaatacca gatgcgactg catctgaaaa tttcttaagt
     4741 gcagcccctt tgtcataatt ttttggcaag gtaatccggc gtccaagcac agtatccagc
     4801 tggtctgccc agacttctgt cagtgcgtgt ttgccgtagg tatctttaga ataagcacga
     4861 attgcagcaa ccttcataga ggggatttca acaacggtga ctggcaccat gatttccttg
     4921 ccctctgtgg gactgttctt gtgatcatcg accatgataa catgcgtcat acctacttta
     4981 tatccagcaa acccctgaag tatcggtacc ccgttataga gaggccacga ttggtatttc
     5041 gggattgggc tctttgcacg ccttcttggg ctgaatgcaa gagaaccacg gcgtggtctg
     5101 tttatttttg gcatgtgtta atcaatcctt tttctgcgtg taggttattc ccattgcgac
     5161 acgaattacg attaatccga gagtgtgata actttgtctg gtttttatgc caacacgcga
     5221 tgccagatga aagcggagca ctcatacaaa aatacaaaac aaaaaccctg tttggggatt
     5281 ttttcgattg tttgtattct gaacatccgt tttttcatct ggctacagac atatggatga
     5341 taatcctcat ccgggagctg atgttcatat tcaccagcga ccatgtctcg cgaaagaaga
     5401 tcctgacaat aacgcatcca tctgcacctt gaccgggcaa tggaacgggc tcgtttgttc
     5461 aacgtcgacc catgcgaagt aatcagatcc cactcagtca gcctgttgtg acgattcaca
     5521 actccttctc gggtttccct ccacaataaa gtggagttca ccaatcgagg attacccacg
     5581 gcacaaccga cttcctgtgt ccggaaggct ggttccgccg tatgatgctg tgaaaatgtg
     5641 cttttgatta gtcagcacat ggatacatca gaggataacc tcatatcggc ttccataatc
     5701 attgtataaa aaagaatata aacatatgcc aatattagga tatcaaaacg ggatgaatac
     5761 gaaataactt tttttatttt ttcgttttag tccatcaaga gttacccaaa atacaattca
     5821 ccaaggtcag taatatatac caaaatgtct tcacacacac aacgtgcttt acagcactgg
     5881 ggacggatct cacggagtcg attgatcagg gaaattgtat cataacttac ttttatgtta
     5941 attttttctg cttcaccaaa aattattgaa ggaacatcaa acagatcagt acctgaaggt
     6001 atagtacaaa gatgagttgc atccagtgtg tagaggaatt caagtgtttc ttttgctcgg
     6061 cggatattct ccattgcgct cttttcaatc gtgcagacat ttgcttttga ggtcgagata
     6121 atatcagcaa tctgctgttg ggtcagccct tgtttacgat acctgagaac ttccatctgg
     6181 cgttcggtga gcagcccatc cttcatagta ttaaatccta tatctgaact taaacacttt
     6241 tcgttaacta tgagagaaac aaacatatgt tgaatttttc tgaaatacgg gggattctat
     6301 gtcaaaaaaa tttttttttt aaaatttttc ggcgagaaag gacatttcaa aatgtttata
     6361 tgaataccgg gtcataataa cgtgttataa aaaaaattta ggtgatatag atggttgtta
     6421 aagtaggagt tgccaaactc ggcaatatag caagtggtgt aatggcagaa ctgcttctcg
     6481 atgagagagc agaccgtgaa gacatgcaga cattcatggc gaccagtggt acgaaactcg
     6541 agccggcaga tgttgaccgt gtggtttcaa acctcaaggc atataagccg gacttctgta
     6601 tcgtagtttc ccccaacggg gttctccccg gaccaaccgg tgcccgcgaa cagctcgctg
     6661 ctgcaggtat cccggttatc atcatcactg atgatgtaac gaccaagaaa gaatgggaag
     6721 gagtcaaggc aagcaagttc gggtacatca tcatgaaggc agactccatg atcggtgcac
     6781 gccgcgagtt cctcgaccct gtcgagatgt cagactacaa cgggaacctc gtcaaagtgc
     6841 tggcacttac tggtgcattc cgcaaactcc agctcgcagt cgacaaggtc attgatcagg
     6901 tcaaggccgg aaagagtgga gatgcactcg agctcccgaa aatcattatg acgaccgaca
     6961 aggctgtcga tggtgaattc acgaacccct tcgcactggc aaaggcacgc gctgcccacg
     7021 agatcgcaat ggcagttgca ggccagaacg tcaagggctg cttcatgacc aaggaatggg
     7081 agaagtacat cccgatcgtc gcctccgcgc acgagatgat gcgcgtggcc gccgtgctct
     7141 gtgacgaggc ccgcgagatc gagaaggccg gcgacggcgt cctccgcaag ccccacaaga
     7201 aggacggcac
//
LOCUS       DASRLV010000167         7858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_57940,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000167 DASRLV010000000
VERSION     DASRLV010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 7858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7858
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57940"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..679
                     /locus_tag="VFC43_06590"
     CDS             71..679
                     /locus_tag="VFC43_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HZK30882.1"
                     /translation="MENKFAEMLSVKKSVLLLVDYQARMVYGTESHDRTHLKNNILAL
                     SKGAEILHIPTVLTTHNAANNGPFLQEIVEMFPDLSVIDRKIPGFDALDDKEVLEAVR
                     KTGRKQLVISGLWTSMAMTHTALHALREGFEVFGVMDAAGSESLYAHDMAVLRMIQAG
                     VVPCTWMQTVSEWMNTWHNPKAAELFTKVYCVYSSFFGQKTR"
     gene            811..1548
                     /locus_tag="VFC43_06595"
     CDS             811..1548
                     /locus_tag="VFC43_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30883.1"
                     /translation="MNKIPLETTDDESSIMFRDCFSVRYIQSAALLCRLGYRIEKHFL
                     DCGEIPEEERLRHEVYILNSVLSSVAFLESTINELYADAADDAYFFTDVKNENLLKTI
                     GEKWRNEKNFDRAPLVNKYQKILFIAGKPPSDEDDPVFSNIRTLIEIRNYLMHYKREW
                     VVVQKGEMYGESQETHGEKLERLLRNKFFYNPLAQKNRPFFPDTCFGHGCAEWAVNNS
                     LAYTDEFFSKLEFPPPYDGVRNELATK"
     gene            complement(1559..1954)
                     /locus_tag="VFC43_06600"
     CDS             complement(1559..1954)
                     /locus_tag="VFC43_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing protein"
                     /protein_id="HZK30884.1"
                     /translation="MPHKCTKCGREYKAGSTEILKGCASCGGKKFLYVKEAEIHKDVL
                     DEKSMDEIADESHEDILEIVEPRKKGQVEMYDRVETIRIISPGTYELNLEKMAQSDQR
                     IVSVGKEGNYIIDLMSMAKDQPKKKKAKK"
     gene            complement(1960..2613)
                     /locus_tag="VFC43_06605"
     CDS             complement(1960..2613)
                     /locus_tag="VFC43_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Era-like GTP-binding protein"
                     /protein_id="HZK30885.1"
                     /translation="MKIFYQTYGTAKKKFSNFLKRFFKKKRTRIGIYGPPNAGKTTLA
                     NRIARDWTGDAVGPVSEIPHETRRARKKEDIIISGANGSTITMDIVDTPGVTTKIDYH
                     EFLEFGMDKDEAIIRAREATEGVAEAMHWLREDIDGVIYMLDATLDPFMQVNIMMIGI
                     IESRKLPVIIVANKIDLAEAAPARIRSAFPQHPVVAISGLEGRNVENLYETMLECFG"
     gene            2865..3431
                     /locus_tag="VFC43_06610"
     CDS             2865..3431
                     /locus_tag="VFC43_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZK30886.1"
                     /translation="MTKTSNGMRFETQIPLRDVMKNNPTMIGIDATVEKAAKAMCVEE
                     VGSVIILEHSKPIGIVTEEDITCKVVAKDLKPSSVQVKEIMSTPLITVSAEKTVGDAA
                     HMMVKHKVRRLPVIDAKKKVIGIVTVRDLLTVSNELNELLADLIEINREEIVEQGMCN
                     RCSQMSDDLKRVDNVMLCSRCREEDNIS"
     gene            3428..4177
                     /locus_tag="VFC43_06615"
     CDS             3428..4177
                     /locus_tag="VFC43_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZK30887.1"
                     /translation="MKTAQDFMIGIPVLKYNDQITKARQILRDDRFREIYIVDAKKNI
                     LGYIDLTDGLRVTATKSNVTVEGYVKEAPTVSPGDTLEQVARVMRDYETDSVAVVDPH
                     HHILGGVLLSDVFPVITSRHELHGIVASHMTKDVIIASLTDTIQKIYTMIMESGFTAF
                     PVVKKKRIVGLISRRDLIRTRRVQSVISQHAQTTIEEIMSKDVITIGPDEPIGSAAEL
                     LVKHDVSRLPVTDGDQIVGIVDRHDVLRGLA"
     gene            4276..5217
                     /locus_tag="VFC43_06620"
     CDS             4276..5217
                     /locus_tag="VFC43_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZK30888.1"
                     /translation="MNQNDRGIKQGDRLLKMQGKLDRGPVEFKSHIVKQEGEIMAIAT
                     CDVISVPPTQSIIGAVEIMTNCGFRRLPVTDAGTKKLRGIITSGDVINFMGGGDKYKL
                     VQVRHGGNLLAAVNESVRTIMTQQITTLSHESRISDAVNMIVEKKIGGLPIVDQDGVL
                     NGIVTERDVLRVLGAERSTLKVEDVMSSSPRVTAPDCPIANVAHDMIQYRFRRLPVVS
                     DDVLYGIVTATDIMKYLGSREVFSRLTTGNVADVMGLPVRTLVAGDLFTTTPMQSINQ
                     AAREMLTRNIGALPVIEGSRLIGLVTEFDLVRAFAKG"
     gene            5223..6062
                     /locus_tag="VFC43_06625"
     CDS             5223..6062
                     /locus_tag="VFC43_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZK30889.1"
                     /translation="MFARDVMTAPVFFVSPHETIAYARNLMVKHKISRLLVMEEGRLT
                     GILTKKDIAYHIRQGEPSWRRRPVDQIPVGAFTTANPIVVAPDTGIKKITGIFAGKNI
                     SSVPVVEAGTVVGIVTKTDLMKSALVRNLTCPVEDIMEDVATVSRYHSLDHVITLMSE
                     RHDKVVVLNNDGTIAGIITETNLAFFDNDKKITGVVKKDVTIGRRERADGMSGQSLRS
                     VGSVVAEDVMTSPVITVPIKTLLPDALELMRKNHVNSLVVIENDTISGIIKRDDIIKE
                     VAK"
     gene            6059..6835
                     /locus_tag="VFC43_06630"
     CDS             6059..6835
                     /locus_tag="VFC43_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZK30890.1"
                     /translation="MTKEIFIKDVMAKPATISKSAKITEALDKMLAEGIDPLIAVNNN
                     SIVGTVSRQAIAEKLGSKQNSDLAPSAIHVASVVEEEFTSVYPDESINVLIPLLQHYK
                     LVVVYDSDHKLIGQVSAGDLLKKYQPDTGIESVIEKVITIEADERVVHLRRRMLDDKI
                     TRFIVTDLGKNIGIVTETDVAIAMRKFRESIRDQHQDHRIRNVIVRDIMTAPLVSVES
                     TASVSDVIDLMLKKHISSVPVMVKGRLSGIVTRKSLVNAL"
     gene            complement(7004..7768)
                     /locus_tag="VFC43_06635"
     CDS             complement(7004..7768)
                     /locus_tag="VFC43_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZK30891.1"
                     /translation="MLSEFEILIAGLAAAAAGLINALAGGGTLVSFPVLIALGVPDIA
                     ANITNTVALCPGYLGGAITQREDLRGQGRRLWILLPAGIIGGIAGSILLLLGNERVFH
                     VLVPFLILLAALLLAVQDRVRDWVRERSHSAESGNVGGTAILPVGLAAIYGGYFGAGI
                     SVIVLAVLGLFLDDTLTRLNALKQCISLSINVSAAIFFLFSGMVIWPLAVVMAVGALV
                     GGAVGGRIAGRIDPVLLRWTVVILGSAVGILLLVSL"
BASE COUNT         2149 a         1862 c         1918 g         1929 t
ORIGIN      
        1 gtttttagtg tttttcatgc gatatggtgt cacgaaaaag gaaattattg tgttttaaag
       61 gtagttgaaa atggagaaca aatttgcaga aatgctgagc gtcaaaaaaa gcgtattact
      121 tctggtggat taccaggccc ggatggtgta cggtactgaa tcacacgacc gcacgcacct
      181 gaaaaacaat atattggcgc tgtcaaaagg tgctgaaatt ctccatattc ccacggttct
      241 taccacacac aatgctgcca acaacggccc ttttttacag gaaatcgtgg aaatgtttcc
      301 ggacctgtca gtaattgatc ggaagattcc gggttttgat gcgctcgatg ataaggaagt
      361 actggaagca gtcaggaaga ccggacggaa acaactggtc atttctggtt tgtggacgag
      421 tatggcgatg acccataccg cgctccacgc cctgagggaa ggttttgagg tatttggtgt
      481 gatggatgca gcgggttcag aatcgttgta tgcccatgat atggcagttc tccgtatgat
      541 acaggctggt gtggtaccat gcacatggat gcaaacggta tccgaatgga tgaatacctg
      601 gcataaccct aaagctgccg aattatttac aaaagtctat tgcgtataca gcagtttctt
      661 cggccagaaa acccgttaaa ttcttttttg tgctgactgc tggttccggc aggtgaatcc
      721 ggtacaattc acccgtccag ttttatcaag gaatatgtca ttgaatatcc ccctgtcttt
      781 tttgctcatg aaatgaaaag aaatgtaaga atgaataaaa tacccctgga aacaactgac
      841 gatgaatcat ccataatgtt ccgggactgc ttctctgtcc ggtatatcca gtctgctgca
      901 ttattgtgca ggctggggta ccggattgaa aagcattttt tggactgcgg agaaatcccg
      961 gaagaagaac gcctccggca cgaggtatac attctcaact ctgttctctc atccgttgca
     1021 ttccttgaat ccactatcaa tgagctctat gcagacgctg cagatgatgc ctattttttc
     1081 accgatgtaa aaaacgaaaa tctcttaaaa accattggtg agaaatggag aaacgaaaag
     1141 aactttgatc gcgctccact ggtaaacaag taccagaaaa tactcttcat cgcaggcaaa
     1201 cccccctccg atgaagatga tcccgttttt tcaaatatcc ggactcttat tgaaatccgg
     1261 aattacctca tgcattacaa acgggagtgg gttgttgtgc aaaaaggtga aatgtacggc
     1321 gagagccagg agacgcatgg agaaaaactg gaacggttgc tcaggaacaa atttttttat
     1381 aaccccctgg cacaaaaaaa ccgtcctttt tttccggata cctgctttgg gcacggctgc
     1441 gcagaatggg cggtgaataa cagccttgca tataccgacg aatttttcag caaacttgaa
     1501 tttcccccac cttatgacgg ggtaagaaat gaattagcaa caaaataaga aaaagggact
     1561 acttctttgc cttcttcttt ttcggctggt cttttgccat ggacataagg tcaatgatat
     1621 aattgccctc tttaccgaca cttacgatac gctggtcgct ctgcgccatc ttttcgagat
     1681 tgagctcata cgtacccggt gaaataatac gaatcgtctc cacccggtca tacatctcta
     1741 cctgcccttt tttcctgggc tctacaatct cgaggatgtc ctcatgagac tcatcagcaa
     1801 tctcatccat ggatttctca tcaagcacat ccttatggat ctctgcctct ttgacgtaga
     1861 ggaacttttt gcccccacag cttgcgcacc ctttcaggat ttccgttgaa ccagccttgt
     1921 attcccgtcc gcatttagtg catttgtgcg gcataccagt cacccgaagc attcaagcat
     1981 agtctcgtac agattctcaa cattccgtcc ttccagtccg gagatagcga caaccgggtg
     2041 ctgcgggaat gcacttcgga tccgtgcggg tgctgcttcc gcaagatcga tcttatttgc
     2101 aacaataatc accggtagtt tacggctctc aatgataccg atcatcatga tattaacctg
     2161 catgaaggga tccagtgttg catccagcat gtagatgact ccgtcaatat cctcacgcag
     2221 ccagtgcatt gcttcggcta caccctcggt tgcttcccgg gcacggatga tcgcttcatc
     2281 cttgtccata ccgaattcca ggaattcgtg ataatcaatt tttgtggtaa cacccggtgt
     2341 atctacaata tccatcgtta ttgtactgcc atttgcaccg gagatgatga tatcctcttt
     2401 ctttctcgcc cttcgggtct cgtgcggtat ctcgctcacc gggccaacag catcgcctgt
     2461 ccagtcccgg gctattcggt ttgcgagggt tgtcttacct gcattgggtg gaccatatat
     2521 gccgatacgg gttctcttct ttttaaaaaa ccttttaagg aaattggaaa atttcttctt
     2581 tgcagtacca taggtctgat aaaatatttt catgggcaac ctccatgact aagcatcgta
     2641 caaaggtaca aagaattcac cacaattcgt tagtagtgta tagaaatcgc tctttattat
     2701 gtttcttata atacgctttc gtgaaccccc gtcaacggtt tcccggcacc cctcaatatt
     2761 actgcgctgc atggagggtt gcctttgcca aattgtattt atacaagtga aggggagtat
     2821 gctaattgta atcatcagaa aaaagcactt caaggaggtg actcatgacc aagacatcca
     2881 atggaatgcg gtttgagacc cagattccgt tacgggacgt gatgaaaaac aacccaacca
     2941 tgataggaat tgacgcgact gtcgaaaaag cggcaaaagc catgtgtgtt gaagaggttg
     3001 gcagtgtcat tattctcgag cacagtaaac caatcggtat cgtaactgaa gaagacatta
     3061 cctgcaaggt ggtggcaaaa gatcttaaac caagtagtgt acaggtcaaa gaaatcatga
     3121 gcacgccact cattacggtg agtgcggaga aaactgttgg cgatgccgcc catatgatgg
     3181 tgaagcataa agtaaggaga ctacccgtca tcgatgcaaa aaagaaggtc atcgggattg
     3241 tcactgtgag ggatctcctt accgtatcca acgaactcaa cgagctcctg gccgatctca
     3301 tagagattaa ccgagaggag attgttgaac agggaatgtg caaccgctgc agccagatgt
     3361 ctgatgatct caaacgggtt gacaatgtca tgctctgttc ccgttgtcgt gaagaggata
     3421 atatttcatg aaaacagcac aggatttcat gatcgggatt cctgtgctga aatataacga
     3481 ccagataacc aaggcacggc aaatcctgcg agatgaccgg ttccgggaaa tttatatcgt
     3541 ggatgcgaaa aaaaacatcc tcggctatat tgatcttacg gatgggttgc gggtaacggc
     3601 aacaaaatcg aatgtgaccg ttgaaggcta tgtcaaggaa gctcccacgg tcagcccggg
     3661 tgatacactt gagcaggttg cgagggtgat gcgggattac gagaccgaca gtgttgcggt
     3721 agtggatccc caccatcata tcctcggcgg agtactcctt tcagatgtct tccctgtaat
     3781 aacttcccgg catgaacttc atgggatcgt tgcttcccac atgaccaagg atgtgattat
     3841 tgcgagtctg acagatacga tccagaagat ttacaccatg atcatggaga gcggttttac
     3901 cgcgtttcct gttgtaaaga aaaagcggat tgtaggatta atttcccgtc gcgaccttat
     3961 caggacacgg cgggtccagt cggtaatttc ccagcacgcc caaacaacca tcgaagagat
     4021 catgtctaaa gacgtgatca cgatcggtcc tgacgaacct atcggctctg cagcagaact
     4081 gctggtaaaa catgacgtga gccggctccc tgtcactgac ggggaccaga tagtaggaat
     4141 tgttgaccgg cacgatgtgt tgagaggtct tgcgtgaacc attacacgaa atggcgtgca
     4201 attttgtgaa aactgcacga tgtactattt caaatacaag tatatcaggg tcattgaacc
     4261 attcggaggg ggataatgaa ccagaatgac cggggcatca aacagggtga cagactcctg
     4321 aagatgcagg gaaaactcga ccgtgggccg gttgagttca agtctcatat cgtcaagcag
     4381 gaaggcgaga tcatggcaat cgcaacatgt gatgtcattt cagtcccgcc cacccagagc
     4441 attatcgggg cggtggagat catgacaaac tgcggtttcc gccgtttacc tgttactgat
     4501 gccggcacaa agaaactgcg ggggatcatc acttcaggtg acgtaatcaa tttcatgggg
     4561 gggggagaca agtataaact cgtacaggtg cggcatggtg gcaacctgct tgctgctgtc
     4621 aacgagagtg tacgcaccat tatgacccag cagatcacca cgctctccca tgaatcccgg
     4681 atcagcgatg cagttaatat gattgtcgaa aagaagatcg gggggttacc tatagttgac
     4741 caggatggtg tgctgaatgg tattgttacc gaacgggatg tgctgcgggt gcttggtgca
     4801 gagagaagta cccttaaagt tgaggatgtt atgagttcat ccccaagggt gacggctccg
     4861 gactgcccta tcgcgaacgt cgcccatgac atgatacagt acaggttccg cagattaccc
     4921 gtcgtgagcg atgatgtgct ctatggtata gtgactgcca cagatatcat gaaatatctc
     4981 gggagccgtg aggtcttttc ccggctgacg accggcaatg ttgctgacgt catgggatta
     5041 ccagtccgca cactggttgc cggggatctc ttcacgacta cgccgatgca gagtatcaac
     5101 caggccgccc gcgagatgct gaccagaaac atcggggcat taccggtgat cgagggctcc
     5161 cggctgattg gtctggtgac cgagttcgat cttgtccggg catttgccaa gggatgactg
     5221 ccatgtttgc acgcgatgta atgacagcac cggtattttt tgtctcacct catgagacga
     5281 tcgcttacgc caggaacctg atggtgaagc acaaaatttc ccgtctcctt gtgatggagg
     5341 agggcaggtt gacaggaatt ctcaccaaaa aagatatcgc ctaccatatc cgccagggag
     5401 aaccctcatg gcgccggcgc cctgttgacc agattcccgt gggagcattt acaacggcta
     5461 atccgatcgt tgttgcaccc gatacaggga tcaaaaaaat caccggcatt ttcgcgggaa
     5521 aaaatatcag ttctgttccg gtggttgagg caggaactgt cgtgggaata gtgacaaaaa
     5581 cagatctgat gaaatccgcc cttgtccgga atcttacctg tccggtcgag gatatcatgg
     5641 aggatgtggc aacagtcagc cgctaccatt cgctcgacca tgtaattacg ttgatgagcg
     5701 aacgccatga caaggttgtt gtcctgaaca atgatggtac aatcgccggg ataatcacgg
     5761 agacgaacct tgcgttcttc gataatgata aaaagattac cggggtggta aagaaagatg
     5821 taacgatagg aagacgcgaa cgggcagacg ggatgagcgg gcaaagcctg cgttccgtcg
     5881 gatcagtggt tgcagaggat gtaatgacca gcccggttat taccgtgcca ataaaaacct
     5941 tgcttccaga tgcccttgaa ctgatgagaa aaaaccacgt caacagtctt gttgtaatcg
     6001 aaaatgacac gatatccgga atcataaaac gggatgatat cataaaggaa gtggcgaaat
     6061 gacaaaagag atttttatca aagatgtgat ggcaaaacca gcaaccatat ccaaatcggc
     6121 aaagattacc gaagcacttg ataaaatgct tgccgagggt attgatcccc tcattgcagt
     6181 caataataat tccatcgtgg gtacggtctc ccgacaagcg atcgcggaga aactggggag
     6241 taaacagaac tccgatctag caccttctgc tatccatgtt gcaagcgtgg ttgaggagga
     6301 attcaccagt gtctatcctg atgaaagtat taatgttctc ataccgctgc tccagcacta
     6361 caagctggtc gttgtgtatg acagcgatca taagttgatc ggccaggtaa gcgccgggga
     6421 tctcctgaaa aaataccagc cggataccgg tattgaaagt gtaatcgaga aggtgatcac
     6481 gatcgaagcg gatgagcgtg tggtccacct gcgccgccgg atgcttgatg acaagatcac
     6541 acgtttcatc gtaaccgatc taggaaaaaa tattggaatc gtgacagaaa ctgacgtagc
     6601 aattgcaatg cggaaattcc gcgagtcgat cagggaccag caccaggatc acaggatacg
     6661 gaatgttatt gtaagggaca ttatgactgc accactcgtc tcggtcgaat cgaccgcaag
     6721 tgtgtctgat gtgatagacc tgatgttaaa aaaacacatc agttcggtcc ccgtgatggt
     6781 caaaggcaga ctttccggta ttgttacccg aaagtcactt gtcaatgcac tctaacccct
     6841 ctcgtttttt tcctctctcg ttattttata tgcctgagtt caatctgcct gaccggggat
     6901 gcacattttc aagatctgaa actaatgatt attctaccat tttcccagaa aaaaatcaaa
     6961 ccgtacctta aaaaaacaat ggtcattccg cagtgtagac tgattaaaga ctgactagta
     7021 ataaaattcc gactgcactt cccagtataa caacggtcca ccgcaacagg acaggatcaa
     7081 tccgcccggc aatccttcct ccaacagcac ccccaaccag cgccccaacc gccattacca
     7141 ccgctaacgg ccagataacc atgccggaaa aaaggaagaa tatcgctgca gagacattga
     7201 tactcaggga aatgcattgt ttaagcgcat taagccgggt cagggtatca tcaaggaaga
     7261 gtcccagcac cgcgagaacg atcacactta tccccgcacc gaaatatccg ccatagatcg
     7321 ctgcgagacc gaccggcagg attgcggttc caccgacatt ccctgattca gcggaatgag
     7381 accgttccct gacccagtcg cggacccggt cctgaactgc cagcagcaag gctgcaagaa
     7441 gaataagaaa ggggacgagg acatgaaata ccctttcgtt tccaaggagg agcagtatac
     7501 tcccggctat gccgccaatg atacctgccg gaaggaggat ccaaagtcgc cgcccctgac
     7561 cgcggaggtc ctctctctgg gtgattgcgc caccgaggta tccagggcat aatgcaactg
     7621 tgttggtgat attggcggca atatccggaa ctcccagtgc gatgagcaca ggaaaagaga
     7681 caagcgtccc tcccccggca agggcattaa ttaaaccagc ggctgcagct gcaaggccgg
     7741 cgatgaggat ttcgaattcg gagagcatgt gaggcgcccg ttattcccgc ttttttcagc
     7801 caggatatgc catgagatcc tgcgctttta tgatctttac cccgatatta gccatatc
//
LOCUS       DASRLV010000168         7306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_549090,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000168 DASRLV010000000
VERSION     DASRLV010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 7306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7306
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549090"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VFC43_06640"
     CDS             complement(<1..755)
                     /locus_tag="VFC43_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZK30892.1"
                     /translation="MKDIIIKGARQHNLKNISVTIPRDKLVVITGVSGSGKSTLAFDT
                     LYAEGQRRYVESLSSYARQFLGIMQKPDVDSIEGLSPAISIEQKTTSKNPRSTVGTVT
                     EIYDYLRLLYARIGIPFCPEHNIPIAAQTPDKIADQIAAEHPGQVTVLAPIVRQKKGT
                     YQQLLRDLDKEGFARVRVNGKVIRTDEEISLDRYKKQDIEIVIDRLDSSDRSRLTEAV
                     ENCLKKSEGLVLVADEGGQESTYSSLMACPVCG"
     gene            complement(1288..1425)
                     /locus_tag="VFC43_06645"
     CDS             complement(1288..1425)
                     /locus_tag="VFC43_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30893.1"
                     /translation="MPTSKKQLVKLNQVKKAKAEELSKQAAAGSQEAKKKLKKLEKKR
                     K"
     gene            complement(1588..2979)
                     /locus_tag="VFC43_06650"
     CDS             complement(1588..2979)
                     /locus_tag="VFC43_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZK30894.1"
                     /translation="MLSVLLVDDEPALLEVLKPFIERSGEMRVHTAQSAAEALKILQE
                     NSFDAIILDYSLPDISGIELLKMLRVKGDTTPVIMFTGVGHERTAIDAINYGANFFLK
                     KEEDPQKLFHELSDMVKNAVESKYVGKKLGTSRKILSDMINFSFEPYFAIDVEGKVVA
                     WNAAMEQLTDTTANNIMGKSDYLYSIPFFGTRRKMLINLVFEPDEEIKRLKYMIISRV
                     AKGPVTALTNGVKKDGSGWTLWSKAMPVYDLQGNFIAAVGIVRDVTGTFEDKMIEDQS
                     QAAEGRIPETASRQPSKPVGLFDKILGKSSGKATSYYKEGVNFYVKEKKYTEALVAFD
                     KALEQDDKLPQVWNDRGLCYRDMGDTINALKSLSRAVELEPDNPELLFNLGMTLEMIG
                     TQNMSNKYLDSAIQTFKMVTNKMPNNADAWNHIGICYNQMGQPEESKFYFDRARDIHT
                     WRKHTPIIPKRDI"
     gene            3163..3348
                     /locus_tag="VFC43_06655"
     CDS             3163..3348
                     /locus_tag="VFC43_06655"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HZK30895.1"
                     /translation="MPVVILKIKKGLSRDQKRKIVEEFTRTLVSNVGVTPDLVTVLID
                     EHEMEDIGKAGKLRCDP"
     gene            complement(3717..4883)
                     /locus_tag="VFC43_06660"
     CDS             complement(3717..4883)
                     /locus_tag="VFC43_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30896.1"
                     /translation="MADALITEHAGTRRGEISRFYQYGSGGVRVMDAKKAVKRLYLFD
                     PSSDSMTERDPFRNEKVLRRFVFDRYGMLEETFAFGARPRTFRYENGASRIAVREGGE
                     YGAVGKIFTFEQNGITETAWGRNGEIERVYQFDAGGDAITIRAGGWFGDVDRTIVFSG
                     IEASLFLEPEAFLQFLMFTEWSASDREADVNERVAGIRSREMAGSPRSRFAYTSPGRT
                     PGDGGVAGGRGPDSRIPAQQNDARSGRTSRGTTSDAGIDYIPDADAPGQAVPKAPASR
                     QSSGISFEERWHRPETDDRKMSLGKSVEIPFEERFGSSRGEREELSKGKSVEIPFEER
                     FGSARGEREELSRGKSVEIPLEERFESARREREKLSKGKSAEIPYDERRSGGNR"
     gene            5026..5547
                     /locus_tag="VFC43_06665"
     CDS             5026..5547
                     /locus_tag="VFC43_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZK30897.1"
                     /translation="MSEENMTPEPSLIKPFPEKTIYLANWSARFWAWLVDIILVLLFL
                     NIVRGLFEPFFLLPLLWDFGNKNWGLFTVGFESIFFFAYWTVMEGFRGQSVGKMVMNL
                     KVVNRDGTRINYPTAALESLGKAFFLPLDCLIGWLAMPGTKLRVFNRISNTIVIKADY
                     REPHGILYVREKE"
     gene            5574..5981
                     /locus_tag="VFC43_06670"
     CDS             5574..5981
                     /locus_tag="VFC43_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30898.1"
                     /translation="MKGYVILFIVLFFGILIGGCVSLPTGQTKVTQTPQVTPRVTTLI
                     PAGPTVTVIIKNRAFDPSSTTITVGTTIVWVNEDPTLHRVVHLPTVTQKESFNSGPLS
                     SGQSYRYRFSEKGTYEYGDPNIGGGRTARIIVE"
     gene            6025..6408
                     /locus_tag="VFC43_06675"
     CDS             6025..6408
                     /locus_tag="VFC43_06675"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZK30899.1"
                     /translation="MTTTAVPDNFREPEKADPIKEAGKNGTGTEDRLEYFIRMLTDDD
                     EINRWKAAEALGRLGDPGAVEPLIDALWDDDSRVRLKAAWALGQVGDLRAVAPLKRLY
                     RMEKEDMKESISEAIEEINLRVSRE"
     gene            6518..7081
                     /locus_tag="VFC43_06680"
     CDS             6518..7081
                     /locus_tag="VFC43_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30900.1"
                     /translation="MVEIESLYKKENGMILIEIKLSSVMQLFNSFDPAPFHEKELDSA
                     AEHYIVDTVKDFPQKTRFKMIIYLPPDIIGTEQAMKISPAIHHHFQYKMLVAERRFRS
                     HFRHGRTTLLIGLTFLTIALLARQQVSSLENHLLAQLFADALLIIGWAAMWEPITVLL
                     YELWPIIQLKKTYEKISTMEIDIVPTQ"
     gene            complement(7085..>7306)
                     /locus_tag="VFC43_06685"
     CDS             complement(7085..>7306)
                     /locus_tag="VFC43_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HZK30901.1"
                     /translation="NPEDFEVLTRMLVHAAGVPLALVLEGGYGPSHGEAVRSIFAALM
                     SEGAVNTRGIPSGHTKSVVSYLKSEHDLF"
BASE COUNT         1767 a         1845 c         1766 g         1928 t
ORIGIN      
        1 atcccgcaga ccgggcaggc catgagggac gagtacgtgg actcctgccc cccttcatct
       61 gcgacgagca cgagtccctc ggacttcttt aggcagttct ccaccgcctc cgtgagccgg
      121 gaccggtcgg aggagtcgag ccggtcgatc acgatctcga tatcctgctt cttgtaccgg
      181 tccaggctga tctcctcgtc ggtcctgatg accttgccgt tgacccggac ccgggcgaaa
      241 ccttccttgt ccagatccct gagaagctgc tggtacgtcc ccttcttctg ccggacgatg
      301 ggggcaagca ccgtgacctg cccggggtgt tcagccgcaa tctggtcggc gatcttatcg
      361 ggagtctggg ccgcgatggg gatattgtgc tcgggacaga acgggatccc gatccgggca
      421 tagaggagac ggaggtaatc gtagatctcg gtgacggtgc cgaccgtgct ccgggggttc
      481 ttggaggtcg tcttctgctc gatggaaatg gcaggcgaga gcccctcgat ggagtcgacg
      541 tccggtttct gcatgatgcc gaggaactgc cgggcgtacg aggaaaggga ctcgacatac
      601 cgccgctgcc cttcggcata gagtgtatcg aacgcgagtg tggatttgcc ggagccggag
      661 actcctgtta tgacaactaa cttgtcccgg gggatcgtga cgctgatatt tttgaggttg
      721 tgctggcggg cacctttgat gatgatatct ttcattttac gtggggtaca gattggtgcc
      781 ggtatttaaa gaagtgagga ttgaggatgg tgaaagtgtg gagggcaaaa gtcacggccg
      841 gatggagttc aggacgtttt gggtcttggc cggtggccgg attcgaggtt taggataatt
      901 ttctccggcc tttccgtacc gctctcccca gtgccctcag taccagagaa gcaacgacgg
      961 cctcggcacc aatccggttc cgggtccgtc aatcaaagag atgatgggtc cgcaccacca
     1021 tgaggccggt actgggggaa gccggaattt attatcccgg agaaaaattg gatagtctgt
     1081 gggaataatc tgctaccacg tgggttaaag aatgagtaaa atttgaccac ttctacccga
     1141 attatgatga aatagaggac ctccaatttt agtgtttggt gctttaaaat cgagtaaagg
     1201 taatttcgga tacggatacc tctactataa agacataagc gaaagccgcg gaagcctaaa
     1261 aaaagagtga ggtagcttcc aggcggctca cttgcgcttt ttctcaagct tcttgagctt
     1321 cttcttggcc tcttggctac ccgcagccgc ctgcttcgag agctcttcgg ccttggcctt
     1381 cttcacctgg ttcagcttca ccaattgttt cttactcgtt ggcatacaat cacctcactt
     1441 tcgccttttg ctgattctat tttagctttt tttcttctta aaatatccct gtatcattga
     1501 accggtattt aaagaagtaa agaatgtgct acctgaaagt atattataag aatatgggtt
     1561 atcaggtatt ggatttctac cggaagatta aatatcccgc tttggaatga tgggagtatg
     1621 cttcctccag gtatgaatgt cacgagcacg atcgaaatag aattttgact cttcaggttg
     1681 tcccatctga ttgtagcaaa tgccgatgtg attccatgcg tcagcattgt tgggcatctt
     1741 gtttgtcacc atcttgaatg tctgaattgc cgaatccagg tatttgttac tcatgttctg
     1801 cgtaccgatc atttcaagag tcatccccag attgaaaagc agttccgggt tgtcaggttc
     1861 aagttccacc gcccgggata atgatttgag tgcatttata gtatctccca tatcccggta
     1921 acagaggccg cggtcattcc atacctgggg aagtttatca tcttgttcca gcgccttatc
     1981 gaaagcaacg agggcttcag tatatttttt ttccttcacg tagaagttaa ccccttcttt
     2041 gtaatatgaa gtggctttac cagatgattt tccgagaatc ttgtcgaaca aacctaccgg
     2101 ttttgatggt tgccgggatg cagtttctgg aatacgacct tccgcggcct gggattgatc
     2161 ctcgatcatt ttatcctcga atgtaccggt cacatcacgc acaattccga cagcggcgat
     2221 gaaatttccc tgcaaatcat acacaggcat tgcttttgac cagagagtcc atccactccc
     2281 atcctttttt accccatttg ttaacgcggt aaccgggcct tttgcaaccc tgctgattat
     2341 catatacttc agacgcttaa tctcctcatc cggttcaaat acgagattta taagcatttt
     2401 tctcctggtt ccaaaaaaag gaatcgagta gagataatcg ctttttccca taatgttgtt
     2461 tgccgttgta tctgtcaact gttccatcgc agcattccac gccacaacct tcccttcaac
     2521 atcaatggca aaatacggtt caaaggaaaa attgatcata tcagataaga ttttcctgga
     2581 tgtaccaagt ttttttccga catatttgct ttcaacagca tttttgacca tatcgctcaa
     2641 ttcatggaac agcttttgcg ggtcttcttc tttttttaag aaaaagtttg ctccataatt
     2701 tattgcatca attgccgtgc gttcatgtcc tactccggta aacatgatga ccggtgtggt
     2761 atcccctttc acgcggagca tttttaataa ttctatacca ctgatatccg gtaaactgta
     2821 atcaagaata atggcatcaa aagagttttc ctgtagaatt ttaagtgcct cagcagctga
     2881 ttgtgcggta tggactctca tttccccgga tcgctcaata aatggtttta gtacttccag
     2941 caatgctggt tcatcgtcga ctaaaagaac ggaaagcaat atagatctac ccttcttatt
     3001 aattgaaata aaccaaatga taaatcaagt ggttcagaat gagatgaaat acccgatgag
     3061 gggtgttttt gagcaaccat ctgtttatgc aaaacctcaa aaatacttcc ccgacatgat
     3121 cctaaatact aaaaactccc cataattgct ggaaggttta tcatgccggt tgttatcctg
     3181 aaaatcaaaa agggcttgtc aagagatcag aaaagaaaaa ttgttgagga atttacccgg
     3241 acactggtga gtaacgtggg agttaccccg gatcttgtga cagtactcat tgatgaacac
     3301 gagatggaag atatcggtaa agccggaaaa ctccggtgcg acccgtaact tttctctttt
     3361 tttcggttct tttaaaaaaa atcttgttgt ggttaaaata taccccctct tgagccacca
     3421 gtccccgttg ggggacgggg tgcagtgtta tggctgcaag gtaatccgtc gtcatggctc
     3481 cggggatgcc ccggtggcgg gggcaaagcc caaaaagaga atcagaattt tcttcttgag
     3541 ttcttcagag caccttcttt ttgttttttc gaaaaatcac cggaataagg agcgaaaaag
     3601 gggacttata cctctgagag agattgggct ggtattactg gtaaacagga ttgtgcagat
     3661 caggtcagaa atccggttca tggatatttc accagagccg gattttaaaa aatttttcag
     3721 cgattcccgc cactcctgcg ctcatcatac gggatctcag cacttttgcc ctttgagagc
     3781 ttctcccgtt cacgacgtgc gctctcaaag cgttcctcga gcgggatctc gacactcttc
     3841 ccccttgaga gctcctcccg ttcaccacgt gcactcccga atcgttcctc gaacgggatc
     3901 tcgacactct tcccttttga gagctcctcg cgttctccac gtgaactccc gaatcgttcc
     3961 tcgaatggga tctcaacgct ctttcccaaa gacatcttcc ggtcatccgt ttccgggcga
     4021 tgccagcgtt cctcgaagga gatcccgctg ctctgccggg aggccggtgc ctttgggaca
     4081 gcctgcccgg gagcgtctgc atcggggata taatcaatac cggcatccga cgtagttcca
     4141 cgggaagtcc tccctgatcg tgcatcattc tgttgtgccg ggatccgcga gtcgggtccc
     4201 cgtccgccag ctactcctcc atcccctgga gtgcgccccg ggctggtata cgcgaaccgg
     4261 ctccgggggg atcctgccat ttctctgctt cggatacctg ctacccgttc attcacgtcg
     4321 gcttcccggt cactggcgct ccactcagta aacatcagga actggagaaa ggcttcgggt
     4381 tcaagaaaca gtgaggcttc gatcccggaa aaaacaattg tcctgtctac atccccgaac
     4441 catcctcctg cccgaatggt tatcgcatcg ccgccggcat caaattggta cacgcgctca
     4501 atctccccgt tgcgccccca tgcggtctcg gtaatgccat tttgttcaaa cgtgaaaatt
     4561 ttcccgacag ccccatactc cccaccctca cggacggcta tccgggaagc accgttctcg
     4621 taccggaagg tccggggacg tgctccgaat gcaaaggtct cttccagcat accgtaccgg
     4681 tcaaagacaa aacgccggag aaccttttcg ttacggaacg gatctcgttc tgtcatactg
     4741 tccgatgacg gatcgaagag gtaaagccgc tttactgcct tctttgcatc catcacgcgg
     4801 accccgccac tgccgtactg gtaaaaccgc gagatctcac cccgccgtgt accggcatgc
     4861 tccgtgataa gcgcgtcagc catggttgat attcactccc gtcaagaatc gttttttaac
     4921 gtactgtgtc tgacgggaaa tagtttcttc ttaccggcgt tttcctgcgg gttcctgatc
     4981 aaagagtaac cttgattcgt tcaggatgcc aacacctttt ttgcaatgtc tgaagaaaat
     5041 atgactcctg aaccgtccct gataaaaccg tttccggaaa agaccatcta tcttgccaac
     5101 tggagtgcac ggttctgggc atggcttgtt gatatcatcc tcgtcctcct ttttttaaat
     5161 atcgtacgcg gattgttcga gccgttcttt ttattacccc tgctctggga tttcggcaac
     5221 aagaactggg gactttttac ggtcggattt gaatccatat tcttttttgc ctactggacg
     5281 gtgatggaag gtttccgggg gcagtctgtt ggtaaaatgg tcatgaacct gaaagttgtg
     5341 aacagggacg ggacgagaat taattacccg actgccgccc tcgagagcct ggggaaagct
     5401 ttttttctcc ctctcgactg cctgatcggc tggctcgcga tgcccggtac aaaattgcgg
     5461 gtgttcaacc ggatttcaaa cacaatcgtg atcaaggcag actaccggga accccacggt
     5521 atcctctatg tcagagagaa agaataatca gtaatactct ctttttactt ttcatgaagg
     5581 gatatgtaat tctgttcatc gtattgttct ttggaatttt aatcggcggt tgtgtatctc
     5641 tgccgaccgg ccagacaaaa gtgacgcaaa caccccaggt tacccctcgg gttaccaccc
     5701 tgataccggc ggggccgaca gttactgtga tcatcaagaa ccgggcgttt gatccaagca
     5761 gtacaaccat aacagtggga actacaattg tctgggtaaa cgaagacccg acgctgcacc
     5821 gtgttgttca cctgccgacg gtgacacaga aagagagttt caattcaggc ccgctctctt
     5881 ccggacagag ttaccgttac cggttttctg aaaaaggcac gtatgaatac ggggatccga
     5941 atatcggggg cgggcggaca gcacgtatca tcgtagagta acactgatac ctgccctgcc
     6001 gggcaaaaaa aatacgaagc ggcggtgacc accacggcag tacctgacaa tttcagggaa
     6061 ccagaaaaag ccgatcccat aaaggaggcc ggtaaaaacg gaaccggtac tgaggatcgg
     6121 ctggaatatt tcatccgtat gctcaccgat gacgacgaga ttaaccgctg gaaggcagcc
     6181 gaggcgctgg ggagactggg ggatcctggt gcagtggagc cactgatcga tgcactctgg
     6241 gatgacgatt cccgcgtcag gctcaaagct gcctgggcac tcggccaggt tggagactta
     6301 agggcagtag ctccactcaa acgcctgtac cgtatggaaa aagaggacat gaaggagagc
     6361 atcagcgagg cgattgaaga gatcaatctc cgggtcagca gggaataatg ctcttttctt
     6421 ttttgatttt ttatagcaca tcactacatc aataactgtt aaaggtaaag acagatttat
     6481 cgactttgcc ggtatatagc catgcaggat aacagtcatg gtggagatcg agtctctcta
     6541 taaaaaagaa aacggcatga tcctgattga gatcaaactc tcttcagtca tgcagctctt
     6601 caactccttt gatccggcac catttcatga gaaggaactc gacagtgccg cggaacacta
     6661 tattgtagat acagtgaaag atttcccgca gaagacccgg ttcaagatga tcatttatct
     6721 cccgccagat atcattggca cagaacaggc gatgaagatc tcaccggcca tccatcatca
     6781 tttccagtac aagatgctgg ttgcggaaag gagattccgt tcccatttcc gtcatgggag
     6841 gactactctt ctcattggcc tcacttttct tacgattgca ctgttagcca ggcaacaggt
     6901 ctcatccctg gaaaatcatc tgcttgccca gttatttgcc gacgctctcc tgattatcgg
     6961 ctgggcagcg atgtgggaac caatcaccgt gcttctctat gaactctggc cgattatcca
     7021 gttaaaaaag acctatgaga agatcagtac aatggaaatc gacatcgttc ccacgcagta
     7081 accatcagaa gagatcgtgt tctgatttca gataagaaac aacggatttt gtgtgacccg
     7141 agggaattcc cctggtgttc accgcgcctt ctgacataag cgctgcgaat atggaacgga
     7201 ccgcttcacc atgggaggga ccatatcctc cttcgagcac cagtgcaaga gggactcctg
     7261 cggcatggac cagcatccgg gtgagcactt cgaaatcctc cggatt
//
LOCUS       DASRLV010000169         3376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_625061,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000169 DASRLV010000000
VERSION     DASRLV010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625061"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1756
                     /gene="recQ"
                     /locus_tag="VFC43_06690"
     CDS             <1..1756
                     /gene="recQ"
                     /locus_tag="VFC43_06690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HZK30902.1"
                     /translation="IESVIKGNDTLAIMATGGGKSLCYQLPALCLGGLTLVISPLISL
                     MKDQVDDLNARGIPAAAWNSSSDYKERLEIESGLKNNTIPLLFISPEKCTQPNFLAYL
                     RNSHIRLVAIDEAHCISEWGHDFRPEYRQLAVLKKQFPHIPLIALTATAIPQVRKDIR
                     EQLGLTNATEFTGSFNRHNLQYRIIPKKNSLLTLITYIGQHKNDSGIVYCLSKKETED
                     VSEELRKRGYNAIAYHAGLPKPRREKVQDEFIHDNVKIICATVAFGMGINKPDIRYVI
                     HYDIPKSIESYYQETGRAGRDGLASECLLFYSRADVHKVQSLLESDRSDERHVQRALK
                     KLRDMTDFCESTNCRRKYLLTYFGEEYTEKNCGSCDNCDNPRELMDGTEIAQTILECI
                     HQLPSHFGIELITDVLTGSKSAKIRTYQLDTLPSYNSGKDHSRQQFRTWIHELIRQDY
                     LSREGDRYPVIRLSSKSMALLKGQDRVMLPVPEREVSRATVSLQRKDLNTSDEKLFLH
                     LKMLRKSLADQGHIPPYVIFHDRSLKEMARIKPLTSESFRAIIGVGDHKLEKYGPEFI
                     AAIQAFQDSLGHCLEGTS"
     gene            2063..2245
                     /locus_tag="VFC43_06695"
     CDS             2063..2245
                     /locus_tag="VFC43_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30903.1"
                     /translation="MMVTGSTSKDALIAAFKEAQAKRNEISNENRQRILKFNEEGESF
                     NDVITKILDEYEQLKL"
     gene            2728..3204
                     /locus_tag="VFC43_06700"
     CDS             2728..3204
                     /locus_tag="VFC43_06700"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HZK30904.1"
                     /translation="MQKITPFLWFDTHAQEAMDFYVSVFKNAKVISVTRYGEGGPGTK
                     GTLMTAKILLDRQEFVLLNGGPQFSFSQAISFVVNCASQDEVDWFWEKLSKGGEKQPC
                     GWLKDKFGVSWQVVQVVLQEMLTGADVQKSQRVMMALLQMGKIDIQKLKQAYNNAG"
BASE COUNT          972 a          779 c          791 g          834 t
ORIGIN      
        1 catagaatcc gtcatcaaag gaaacgatac ccttgcgatc atggcaacgg gcggggggaa
       61 atcgctctgt taccagttgc cggcactctg ccttggtggc ctgacgttag tgatctctcc
      121 cctgatctcg ctgatgaaag accaggtgga tgacctcaat gcccggggga ttcctgctgc
      181 agcctggaac agttcttcag attataagga acgactggag atcgagagcg gcctgaaaaa
      241 caatactatc cccctcctgt ttatctctcc ggaaaaatgc acgcagccaa attttttagc
      301 ctatctccgg aactcccaca tccgcctggt tgccatcgat gaagcccact gcatttcgga
      361 atggggtcat gacttcaggc ctgaataccg ccagctggca gtcttaaaaa aacagttccc
      421 tcacatcccg cttattgcgc tgactgctac tgctattccc caggtgagaa aggatatccg
      481 ggagcagctg ggtctcacaa acgccacgga atttaccggc agtttcaacc ggcataacct
      541 gcaataccgg atcataccca agaaaaattc cttactaacg ctgatcacct atatcggaca
      601 gcataaaaat gactccggta ttgtgtactg cctgagtaaa aaggagacag aagacgtatc
      661 agaagaactc cgcaaacggg gatacaatgc cattgcctac catgccggtc tcccaaaacc
      721 ccggcgggag aaggtccagg acgagtttat ccacgataat gtgaaaatta tctgtgcaac
      781 cgttgcattc gggatgggca tcaacaaacc cgatatccgg tatgtcattc attacgatat
      841 cccaaaaagc attgaatcct attaccagga gaccggccgt gccgggcgtg atggcctggc
      901 atcggaatgc ctccttttct acagtcgtgc cgacgtacat aaggtccagt ccctcctcga
      961 aagtgaccgc tccgatgaac ggcatgtcca gagagccctt aaaaaactcc gggatatgac
     1021 cgatttttgt gagtccacga attgccggag gaaatacctg ctcacctatt ttggcgagga
     1081 atatactgag aagaattgcg ggtcgtgtga taactgtgac aatccccggg agctgatgga
     1141 cggaacagaa attgctcaaa cgatccttga gtgtatccac cagctaccct cgcatttcgg
     1201 gattgaactc atcaccgatg tcctgacagg ttcaaagagt gccaaaatca ggacttatca
     1261 actcgacacc ctgccttcgt acaattcggg gaaagatcac agcaggcagc agttcaggac
     1321 ctggatccat gagcttatcc ggcaggatta cctctcaagg gagggtgaca gatatccggt
     1381 catccggctc tcttcaaaaa gtatggcgtt gctgaaagga caggaccgtg tcatgctccc
     1441 ggtcccggaa cgggaagtat cccgggcaac cgtatcctta caacggaagg acttgaacac
     1501 gtctgatgaa aaattattcc tgcacctgaa aatgctccgg aaatcccttg cagaccaggg
     1561 acatattccg ccctatgtca tattccatga caggagcctt aaggaaatgg cccggataaa
     1621 accccttacc agtgagagtt tcagggcaat tatcggtgtg ggagaccata aactggaaaa
     1681 atacggcccg gaatttatcg ccgcgatcca ggcttttcag gattcattgg ggcattgtct
     1741 ggaggggact tcataaaata gtatacaatt cggctatgca cttcaaggaa ccggggacag
     1801 tatagaggag gttttaaaaa agtatagtgg gtttgtatgc gtgatgatta cgactatcgc
     1861 agatgaatga ctaaaaaaaa tgaaatatct gatatcctca tatcaatttt caacggtacc
     1921 ctgtttgatg tgttccaaaa ggagtttttg cataaacagg tggtcgccct gtatattgtg
     1981 atattataga tattctttat atatgagttt tatgtaatta ctggatataa tatttgaatg
     2041 tgacgataat tcctgggagc ggatgatggt taccggaagt acttccaaag atgctttaat
     2101 agcagctttt aaagaagctc aagctaaaag aaatgaaata tcaaatgaga atcgtcaacg
     2161 cattcttaaa tttaacgaag aaggtgagtc atttaacgat gtaattacca agattttaga
     2221 tgagtatgag caattgaaac tatagtatct ttttttccct tattccccca ttgatggtgt
     2281 ttcaaaattt gtttttcagg aatgatttta tgtatttcct attcgataga ccatcagagc
     2341 aatgaatcct gtttctattg cactccaagg atgagctcat tccatgagat aacctttgaa
     2401 acataccggg gctgtggcaa agcagccccc cctcatttac gaaccgttcg acaggtgttc
     2461 atatccgggt tctgctggca gaacttatat tcatggcaga cgtgcgatgc accgatgcct
     2521 ctccagtctt tagtatcact cttaccacta cgcattggta tgacaattga aaggcagttt
     2581 ttgcctaaaa acagaggcaa ccgtcacact ggtcaaaaag atcatcacgg caggaatgac
     2641 agaaaatgaa acgagggcaa aaaaataagt accctgctga tcctgtcaga tcaataattc
     2701 aacagaaaga aaaagaggta aaccggtatg caaaaaatca ccccattcct gtggttcgat
     2761 acccatgcgc aggaggcaat ggatttctat gtctcggtct ttaagaacgc aaaagtcatc
     2821 agcgtaaccc gttacggcga aggagggccc gggacgaaag gtacgctcat gacggcaaag
     2881 atcctcctgg acaggcagga atttgtcctc ctgaacggtg gtccgcaatt ttccttctcg
     2941 caggcaatat cctttgtcgt taactgcgcc tcacaggatg aggtagactg gttctgggag
     3001 aaactctcca aaggcgggga aaaacaaccc tgcggctggc tgaaagacaa attcggtgtt
     3061 tcctggcagg ttgtccaggt tgtcctgcag gaaatgctga ctggtgcgga cgtacaaaaa
     3121 tcacagcggg tcatgatggc actgctccag atgggaaaaa tcgatataca gaagctgaag
     3181 caggcctaca acaatgcagg ataatgttcg gtacgtttgc ctttttaaaa gggatcaacg
     3241 cagccgggct tgttttcttt tggtagatgt gaaaaatata aatcgaaaac aaggtaaaac
     3301 accgttatgg caaacgttat tctgggggtg acaatttcac ccccagaata acgtttgcca
     3361 taacggtgtt ttacct
//
LOCUS       DASRLV010000170         4348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_625145,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000170 DASRLV010000000
VERSION     DASRLV010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625145"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VFC43_06705"
     CDS             <1..906
                     /locus_tag="VFC43_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HZK30905.1"
                     /translation="TIHILMNNHSFVLFIMIQVRPVERVLRAYPTVEGAGVRLHRAFG
                     SYEVPQFDPFLMLDDFRAPRPEDYLLGFPTHPHRGIETVNYMLKGCVEHKDSMGNAGT
                     VNATDVQWMTAGSGIIHSEMPKPANGEMGGFQLWVNLPRSNKMMDPRYQEITHDQIPA
                     VSLRKGVTVRLICGSLQKFTGPVKDIVADPDYLDVSLNPGAVFYHKVKEGYTAFAYVI
                     GGEGTFDPHEGYQMNNRDLVLFGNGEGVYARSSGRGFRFLFFSGKPIHEPVAWRGPIV
                     MNTAEELRTAFREYDEGTFIKKRPQ"
     gene            903..1535
                     /locus_tag="VFC43_06710"
     CDS             903..1535
                     /locus_tag="VFC43_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HZK30906.1"
                     /translation="MTDLLFKEGDETKNPVFLTSSDRVIFLRQETPFRQIFEVPEYRF
                     NDIHPSDRVIDIGANVGAFCIRASQFSPHIVAVEPVTASLLRENILLNGVPVRVMEGA
                     LGTGGPGYVSWDDCGVISPKYPLSRIIEMAGGCDFLKCDCEGAEWCIQPEDLAGIRRI
                     EMELHIPPIGGPPNPDLLDYIGRQYTFEIERKPVHAMLGVMGVLHAERKG"
     gene            1718..>4348
                     /locus_tag="VFC43_06715"
     CDS             1718..>4348
                     /locus_tag="VFC43_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HZK30907.1"
                     /translation="MILIAEPLTIKDLIFHGNQDPDHPALESPGQQSLSYGDLQKQVL
                     LVIKTLNSLGFVRNDRIAVIMPAGPETAVLGIGIMAGFTHTPLNPQYRDHEFQDMFSR
                     LKVKAIVVQKNHKTAARDVALSQKIPIIEITPAPEKAGIFTIGEGIAVGEAEAVFSTP
                     DDTVIVMQTSGTTSVPKIVPLSQKQFCKCAYMYFTRLNLSDKDISLHIVAHFHILGIT
                     HTLLAPLLGGGTVVCTRDYISSDFLSLLKRYRPTFYCAAPAHHKGILHELKKVTAGEL
                     DHHSLQYIRSTSSPLPPQARKELETLFGVPVIESYAMTESPVISINMPHKEGSAGIPM
                     VESLVIMDEQGTILKTFENGEVAIRGDVVFSGYEDPAENVPAFTGGYFRTGDIGYLDK
                     EGYLFLTGRKKELINKGGEKFSPQEIDAVLASYPGVREAMAFRIDDPVLGEDVAAMVV
                     RADEKVSEQDIRRYLLKRLIQFKVPRTIYFVDKIPKGPTGKLLRYAGTERYNSGALSD
                     ENTPPEVPAATLSLEESIVQDKIIQIWNDVLNVQSLSPDDDFFRCGGNSLTAIELLIR
                     IQRVFQITFPPDTIYLYPTIRQQALLICREGTTFSRYHPLIVPIRGNGTLKPLFCFHP
                     LGGWIKEYQYISLFFDPERPVFGIRARGLEPEEKPVLTVKEAAMEYIEAIKTVQKEGP
                     YHLLGFSGGAIYAFELACELQKRGEVVTFLGIIDMSLPAPLKRLFDMSRGQSPNILIR
                     AGFPLYSSLNNWLKKNPKSPLYRLFVKSVRGVARVLLLLKGSSTPPLFASDDKSITDA
                     QRSWIATLPEKQQKLVRTQIRAISLYKPGTFSGDITLFSTGLDPEFYPSDPARGWNSV
                     ITGNTIVTDIPGDHWTLYREP"
BASE COUNT         1134 a         1031 c         1070 g         1113 t
ORIGIN      
        1 acgatccata tcttaatgaa caatcactcc tttgttctgt tcattatgat ccaggtccgt
       61 ccagtcgaac gcgtcctcag ggcatatcca acagttgaag gtgcgggcgt acgtctgcac
      121 cgggcatttg gcagttacga agtcccccag ttcgatccgt ttctcatgct tgatgatttt
      181 cgtgcaccac gaccagaaga ttatctttta ggatttccca cccaccctca ccggggcata
      241 gaaacagtca actacatgct caagggatgt gttgagcaca aagacagtat gggaaatgcc
      301 gggacggtga atgccacgga tgttcaatgg atgacagcag gatccggaat tattcattcc
      361 gagatgccaa aacccgcaaa cggggaaatg ggggggttcc agctctgggt caacctcccc
      421 cggtcgaaca agatgatgga tccacggtat caggagataa cccatgatca gatcccagcc
      481 gtgtcattaa gaaaaggtgt tacagtacgt ctgatctgcg gatctctcca gaagttcacg
      541 ggaccggtca aggacattgt cgctgatcct gactatctgg atgtatccct gaatcctggt
      601 gcagttttct atcataaggt taaggaaggg tatactgcat ttgcctatgt gattggaggc
      661 gaaggaacat tcgatcccca cgaaggatac cagatgaaca acagggatct tgtcctgttt
      721 ggaaatggtg aaggagtgta cgcccgatct tccggcagag ggttcagatt tctctttttt
      781 tcgggaaaac caatccatga gccggtggca tggcgggggc cgatcgttat gaacactgcc
      841 gaagagctcc gcacggcatt ccgggaatac gatgaaggca catttatcaa aaagcgacct
      901 caatgacaga tttactcttt aaagaaggtg atgaaacgaa aaaccccgtt tttttgacct
      961 cttcagaccg ggtgattttt ctccgccaag aaactccgtt ccggcagatc tttgaggtgc
     1021 cggaatacag gttcaatgac atacacccct cggaccgggt aatcgatatc ggggcgaatg
     1081 tgggagcatt ctgcatccgt gcatcacagt tttcccccca tatcgttgcc gtagaaccgg
     1141 tgactgcatc cctgctccgg gaaaatatcc tgctgaacgg tgtgccggtc cgggttatgg
     1201 agggcgcact tggtaccggc ggaccaggat acgtcagctg ggatgattgc ggggtgatat
     1261 caccaaaata tcccctcagc aggatcatag aaatggccgg agggtgcgat ttcctgaaat
     1321 gcgactgtga aggggcagaa tggtgcattc agcctgaaga tctggccggt atccgccgta
     1381 ttgagatgga actgcatatt ccaccgatag gcgggccacc caacccggat ctcctcgatt
     1441 atatcgggag gcagtatacc tttgagattg agaggaaacc cgttcatgca atgcttggtg
     1501 tgatgggcgt actgcatgct gaacgaaagg gatgaaatga cttctcaata gtctgggcat
     1561 gctgttcggg tactgtcgaa aagttaacca tggtctggat ttaaccttcg tgattttgtt
     1621 attggctgat attatgccca gattgaatga tttttttgaa aaacagacct tctttggata
     1681 gaattaatat tctcaaaaaa ccattttttt ctgacaagtg atccttatag ctgaacctct
     1741 tactatcaaa gatctgattt ttcatggtaa ccaggatccg gaccatcctg cactggaatc
     1801 ccccggtcaa caatcactct cctacggcga tctgcaaaaa caggtgcttc ttgtaataaa
     1861 aacgttaaac tcacttggtt ttgtgaggaa tgacagaata gcggtaatta tgccggcagg
     1921 tccggaaacg gctgtcctgg gcattgggat catggcagga ttcacccata ctccacttaa
     1981 tccccaatac cgggatcatg aattccagga catgttttcc cgattgaagg taaaggcaat
     2041 tgtcgtccaa aaaaaccata aaacagcagc aagggatgtg gctttgtcac aaaaaatccc
     2101 gatcatcgaa attactcctg caccggaaaa ggccggcatt tttactatcg gtgaagggat
     2161 agcggttgga gaagcggagg cagtcttttc aacgcctgat gatacagtca tcgtaatgca
     2221 gacttcagga acaacatcag ttcccaaaat cgttcccctt tcccagaaac aattctgtaa
     2281 atgtgcttat atgtatttta cccggttaaa tctctctgat aaagatatca gccttcatat
     2341 tgtagcccat tttcatatcc ttggaattac tcatacgtta cttgccccgc tgctgggagg
     2401 cgggacggtt gtctgcacca gggactatat atcttctgat tttctttctc tgctgaaaag
     2461 ataccggccg acattttatt gtgcagcccc ggcacatcac aaagggatct tgcacgagct
     2521 caaaaaagtg acggctggcg aactggacca tcattcactc cagtatatcc gatcaacctc
     2581 ctctcccctg ccaccccagg caagaaaaga acttgaaacc ctttttggtg taccggttat
     2641 tgagagttat gccatgacag aatcgccggt gatcagcatc aatatgcccc ataaggaagg
     2701 gtctgccgga attccgatgg tcgagtccct tgtcataatg gatgaacagg ggactatcct
     2761 caaaactttc gaaaacggtg aagttgcaat cagaggtgat gttgttttca gcggttacga
     2821 ggaccctgca gaaaatgtac ctgcatttac cggaggatat ttcaggaccg gtgatatcgg
     2881 ttatctcgat aaagaaggct atctctttct tacgggtcgt aaaaaggaac tgatcaataa
     2941 gggtggagag aaattctctc cgcaggagat tgatgcggtg ctggcatcgt atcccggagt
     3001 ccgtgaggca atggcattcc ggattgatga tcctgtcctt ggtgaagatg ttgctgcaat
     3061 ggtcgtacgg gccgacgaaa aggtatcaga acaggatatc cgccgatatc ttctcaaacg
     3121 cctgatccag ttcaaggtcc cccgcactat ctattttgtc gataaaattc ccaaagggcc
     3181 taccgggaaa ctcctgagat atgcaggcac cgaacgctat aattccggcg cattgagtga
     3241 tgagaatacg ccgccggaag tgcctgcggc gaccctgtca ctcgaggaat ctatcgttca
     3301 ggataaaatt atccagatct ggaatgatgt cctcaacgtt cagtccctgt cacccgatga
     3361 tgattttttc cggtgcgggg ggaattcact gaccgcgatt gaactcctca tcaggatcca
     3421 gagagtattt caaataacat tcccgccgga tacgatctat ctgtacccga caatccgcca
     3481 gcaggcactt ttgatctgcc gtgaaggcac aacattttcc cggtatcacc cgctgattgt
     3541 tcccatccgg ggaaatggga ccttgaaacc attattttgt tttcatcctc ttgggggatg
     3601 gataaaagaa tatcagtata tctctttgtt ttttgatccg gagagacctg ttttcgggat
     3661 tcgggcacgg ggactggaac ctgaagaaaa accggtcctg accgtaaaag aagcagcgat
     3721 ggaatatatt gaggcaataa aaaccgtcca gaaagagggc ccgtatcacc tgctgggatt
     3781 ttccgggggt gcgatctatg ccttcgaact tgcatgtgag ctccagaaac ggggtgaggt
     3841 cgtaacattt ctggggatta ttgatatgtc gttgccggct cctctgaaaa gactgttcga
     3901 tatgtccaga gggcagagcc cgaatatact catcagagcg ggctttcctc tttactcttc
     3961 tttgaataac tggctgaaaa aaaatccaaa aagtccgctt taccggctct ttgtaaaaag
     4021 tgtacgggga gttgcccggg ttttgttgtt gctgaaaggc tccagcactc ctcctctttt
     4081 tgcatcggat gataaaagta taactgatgc tcaacggagt tggatcgcca ccctgccgga
     4141 aaagcaacag aaactggtac gaacccagat cagggcaatc agcctgtata aacccggtac
     4201 attttcggga gatatcaccc ttttttctac cgggctggat cctgaattct atcctagtga
     4261 tccggcccgt ggctggaatt cggtcataac cgggaacact atagtcacag atattcccgg
     4321 tgaccactgg accctgtacc gggagcct
//
LOCUS       DASRLV010000171         3728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1027501,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000171 DASRLV010000000
VERSION     DASRLV010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027501"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VFC43_06720"
     CDS             complement(<1..568)
                     /locus_tag="VFC43_06720"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HZK30908.1"
                     /translation="MDDLLRYREIDLVRGIAILMMVVFHTLFDLNFFSVLSVEVSGGF
                     WRYFAFATASLFLLVAGISLTISRAKMPGQVTRAQVIKKFFLRGAGIFLLGLLVTLCT
                     WIYLKEGFIVFGILHLIGVSVMLSPLFFWLKKRAVLPGLLCIIMGYFLAPVNGPIWLL
                     PLGIHPASFWSVDYEPLFPWIGCVLIGMG"
     gene            686..1546
                     /locus_tag="VFC43_06725"
     CDS             686..1546
                     /locus_tag="VFC43_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 8 protein"
                     /protein_id="HZK30909.1"
                     /translation="MTQENDEHIIEAIGRSRIVIRNGRVVEVGEAQITDCPLAKRFAY
                     PIPAITKDSIKMNIEHRIKAFGMCTENREVLDTREFVGFGASELLSFGLKVGIIDAAV
                     LACDGAGTVIVTKPAMAQGIGGRMSGLVKTSPHQSVMDRIEKGGGFVLDRDHARIDQL
                     AGVALAIAEGFRNIAVTVAIPDTAEIIRRIHPETLIFAVHVTGLSQDEAEKLVTASDL
                     VTSCASKTIRETAGAGALLQAGISVPIFAMTQKGKLLILEKIRQSDEPVLVKPTRLPA
                     LGDKQPSPLI"
     gene            1778..>3728
                     /locus_tag="VFC43_06730"
     CDS             1778..>3728
                     /locus_tag="VFC43_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZK30910.1"
                     /translation="MNDPPSDVNENIIDIFVLSVSETVTSLFTEHLEKKGYRVTLFTD
                     GRYLLQTLREGKPNLLICDTSTLDEEGFEVCRQIKTDNDMWVIPVLILAKGSSITDLL
                     RVLDCNADNFITHPFDPAYGISVIDGLLSTPVERQTPDQIKTQFKISHNDQIYVVAAN
                     RRKLLEFLLSSFEITVNQSSELSRIKSEFQMLSESAKNLEDRVREQTRVIDGIRDTLR
                     QKEQKILSLTHQVEEDKKILGQNTEVIEHFSEQLEDDKVLLSTLEKNLRESISQREEI
                     ESALQTEINTLRKQISELSTEVDQTKTSLDTVQGELDDERIHCTSLECTIDLQVQQKE
                     LAEKTLHTLTFEYEQLKSALAEEETRAVSAETELDKVTEEKTLAEDDFTRKISTLNEE
                     IQRQAADQDRIKGELEAEKNQRTGLENTLESLQKEKVRSESSLQSSVNNLTQQLGDLQ
                     VQYDKISAMLESEQVRTESLRKNLDEVVAEKEKTSGALTTENEQLKSALEDERNRASL
                     SEQELHKIVQEKTRSEEELTRSIQGLDETVKDQVAELARLKAGLDDENAKRISAENLA
                     GTVRQEKEQAESSLSSAIAALKGQIGELQEKFDHTRTALSTEENRTKSLKEHIADIVA
                     DKEKAEEMLKTDRDSYKSTFLRLKHD"
BASE COUNT         1157 a          867 c          883 g          821 t
ORIGIN      
        1 gtcccatgcc gatcaacaca cagccgatcc acgggaagag tggttcataa tctactgacc
       61 agaacgatgc cgggtgaatg cccagaggaa gcagccagat cggaccgttc actggtgcga
      121 gaaaataccc catgataatg cacaacaaac ccggcaggac tgctcttttt ttcaaccaga
      181 aaaagagggg tgaaagcatg actgatacac cgataaggtg caggattcca aaaacgataa
      241 atccctcttt taagtaaatc cacgtacaca gggttacaag aagtcccagc agaaaaattc
      301 cggccccccg caggaaaaat ttcttaataa cctgagcgcg ggttacctgt ccaggcatct
      361 ttgccctgct gatcgtgagt gaaatgccgg caacaagtaa aaagagtgat gcagtcgcaa
      421 aagcaaaata acgccagaat ccgcctgata cctcaacgga aagaaccgag aagaaattca
      481 ggtcaaagag cgtatgaaaa acaaccatca tcaggatcgc tatgccacgg accaggtcaa
      541 tctcccggta tcgcaacaga tcatccatgt acattcatcg tcaatagtgt gtgatatctg
      601 ttttgaatgc gtgtttgcag tcttgcaata ttccttaaac ccggacaacc aataaaaact
      661 ccggattaag gcaggatgtg tgtccatgac gcaggaaaat gacgagcata ttatcgaagc
      721 tatcgggcgg agccggattg tcatcaggaa tggcagggtt gtcgaagtcg gtgaggcaca
      781 gattaccgat tgtcctctag caaaacgatt cgcatatccc attcccgcaa tcaccaaaga
      841 ttccattaaa atgaatatcg aacaccgtat aaaggcgttc gggatgtgta cagaaaaccg
      901 ggaagtactt gatacccggg aatttgtcgg tttcggtgca tcagaattgt taagttttgg
      961 actgaaggtg ggaataatcg atgctgcagt actggcgtgc gacggtgccg gaaccgttat
     1021 tgtaacaaaa cctgctatgg cacagggcat tggagggagg atgtccggcc tggtaaaaac
     1081 atctcctcac caatcggtca tggacaggat tgaaaaagga ggaggtttcg tcctcgaccg
     1141 ggaccatgcc cgtatagacc agctcgccgg agtagctctt gccattgcag aaggcttccg
     1201 gaatattgca gtcaccgttg ccatacccga tactgcggaa ataattcgcc ggatccatcc
     1261 ggagaccctg atttttgcag tccatgtgac gggtctttct caggatgagg cagaaaagct
     1321 tgttaccgca tcagacctcg taacctcctg cgcatcaaaa accatacggg aaaccgccgg
     1381 agccggggcg cttctccagg ccggtatttc agttccgata tttgccatga cacaaaaagg
     1441 aaaattactg attcttgaaa aaatacgaca gtctgatgaa ccggtactgg taaaacccac
     1501 ccggcttccc gctctaggtg acaaacagcc aagtccgctg atataaaaca tctctttttt
     1561 ccgtcgttca cttatctcct gatagtccta cgaacccacc tttcggctgt tgggtaaata
     1621 acctgttacc tttccaaaaa ggatttttct atgcgttttt tctcaccaaa aaaagaaatt
     1681 gctgtactac tgaccagaag tgctataatt gagtatacaa gatcatatat taaggtatat
     1741 aattaattga gatttcaatc gagtaagaga gattgcaatg aatgacccac cgtcagatgt
     1801 aaatgagaat atcatagata ttttcgtttt aagcgtaagt gaaacagtga cctcgctgtt
     1861 tactgagcac ctggagaaaa agggatacag ggttaccctc tttaccgatg gccgatatct
     1921 tctgcagaca ctgcgtgaag gaaaaccgaa ccttctcatc tgtgatacct caacactgga
     1981 tgaagaggga tttgaggtct gtcgtcagat caaaacagat aatgacatgt gggttatccc
     2041 cgtccttatt ctggccaaag ggtccagtat cacagacctc ctgcgtgttc ttgactgtaa
     2101 tgcggataat ttcatcacgc acccatttga tcctgcctat ggtatttctg tcattgacgg
     2161 gctgctcagc actcctgtgg aacggcagac gccggaccag ataaagacgc agttcaagat
     2221 cagtcataat gaccagattt atgtggttgc agcaaaccgg cgcaaacttc ttgagttcct
     2281 cctgtcctct tttgaaataa cggtcaacca gtcatcggag ttgtcccgca taaaatcaga
     2341 attccagatg ctttctgaat ctgcaaaaaa cctcgaggac cgtgtccgtg aacagacgcg
     2401 tgttatcgat ggtatccgcg atactctccg gcagaaagaa caaaaaattc tgtcactgac
     2461 tcaccaggtc gaagaagata aaaaaattct tggacaaaac actgaagtca tcgaacattt
     2521 ttcagaacag ctggaggatg acaaggtcct tctttccacg cttgaaaaaa atcttcgtga
     2581 gagcatttca caaagagaag aaattgaatc cgcgctccag actgaaatca atacactccg
     2641 caaacagatc tcagaactgt cgaccgaagt cgaccagacg aaaaccagcc tggatacggt
     2701 gcagggtgaa cttgatgatg agagaattca ctgcacatcc ctggaatgca ccatcgacct
     2761 ccaggtacag caaaaggagc tagcggaaaa aactctccat acattaacgt ttgaatatga
     2821 gcagctgaaa tcggcattgg ctgaggagga aacccgggca gtatctgcag aaacggaact
     2881 tgataaggtg acggaggaaa aaacccttgc tgaggatgat tttaccagga aaatatccac
     2941 gctcaacgag gagatacaac gccaggctgc cgatcaggac cgcataaaag gggaactcga
     3001 agcagaaaag aaccagcgga cgggtcttga aaataccctg gaatcccttc agaaggaaaa
     3061 ggtccggtca gaatcctctc tccagtcatc agtcaacaat cttactcagc agcttggcga
     3121 tttacaggtt caatacgaca agatctctgc aatgcttgaa tccgaacagg taaggactga
     3181 atccctcagg aaaaaccttg atgaggttgt tgccgaaaag gaaaaaacat ccggtgcgtt
     3241 aacaacggaa aacgagcagc tgaaatcggc gctcgaagat gagcgcaacc gtgcatcgtt
     3301 gtcagagcag gaactccata agatcgtcca ggagaaaacc cggtctgagg aggaactgac
     3361 ccgaagcatc caaggactgg atgagacggt aaaagatcag gttgcggaac tggcacggct
     3421 aaaagccggg cttgacgacg agaacgcgaa acggatctca gcagaaaacc tggccggaac
     3481 ggtgagacag gagaaggaac aggctgaatc gtcattgagc tctgcaatag ctgctctcaa
     3541 aggacagatc ggggaactgc aggaaaagtt tgatcatacc cggacagcac tttccacaga
     3601 agaaaacagg accaaatcgt taaaagagca tattgctgac attgttgctg ataaggaaaa
     3661 ggcagaagaa atgttaaaga cagaccgtga ttcgtacaaa tccacgtttt tacggctgaa
     3721 acatgacc
//
LOCUS       DASRLV010000172         3409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_625317,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000172 DASRLV010000000
VERSION     DASRLV010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625317"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1641)
                     /locus_tag="VFC43_06735"
     CDS             complement(<1..1641)
                     /locus_tag="VFC43_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30911.1"
                     /translation="MNLWIRIVVISVFSLMVMEPFASATGIMIHNTDGLKNDSAFRYI
                     DSSTPGPAGRETPQVLVSGPSVSGVLIFPADHIWNTRVDSLPLDSQSSAYTSSIGNNS
                     YLHADFGSGLYEGNLIGIPYNIVNGSQAKRTVSFEYGDESDPGPYPVPDNPLIESGSD
                     HHLLIIDRDTKMLYELFAAEKQDDGSWSAGSGAIFNLSGYTLRPQGWTSADAAGLAVL
                     PGLARYDEVSTGEITHALRFTAPSTRKAYIWPARHYASSNTDPSYPPMGQRFRLKSSF
                     NTSGYPYQAQIVLLALKKYGMILSDNGAPWYITGAPDDRWDNDALHALHQLKGSDFEA
                     VDCSSLVINQDSGQARVNPIVNGTSHHTGVFRNGNWYLDTTRTGTVNKTFHFGTTGDI
                     PVIGDWDGNGISDAGVFRPSNGNWYLDTTRTGTVNKTFHFGTTGDVPVIGDWDGNGIS
                     DTGVFRPANGNWYLDTTKTGTVNKTFHFGTTGDVPVIGDWDGNGISDAGVFRPSTGNW
                     ALETTKTGVVDKAFHFGTTGDVPVIGDWDGNGISDAGVF"
     gene            2012..2221
                     /locus_tag="VFC43_06740"
     CDS             2012..2221
                     /locus_tag="VFC43_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZK30912.1"
                     /translation="MSKDIVSVSDDQTVATVAGIMGEERVGSVIVDGKGKPSGIFTVR
                     ETSSQNSLLRANPLMSRSVTHPHLR"
     gene            2245..2364
                     /locus_tag="VFC43_06745"
     CDS             2245..2364
                     /locus_tag="VFC43_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZK30913.1"
                     /translation="MHKAAKIMAAQHVRRLPLMKKKLIAGIITARDLIEAYAR"
     gene            complement(2470..2658)
                     /locus_tag="VFC43_06750"
     CDS             complement(2470..2658)
                     /locus_tag="VFC43_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30914.1"
                     /translation="MASVIYEGDDFKRMMRKDMLKLQQLVNAKKMGIFEVDYKDGKIK
                     VEIKKRGDDIVVKRWRSS"
     gene            2832..3197
                     /locus_tag="VFC43_06755"
     CDS             2832..3197
                     /locus_tag="VFC43_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30915.1"
                     /translation="MSREKIKVPGKVYEELVALQREIHFTQDHQDTIKKAEDRGYMNA
                     SNWIRQNENAYKIGFAWGFEPMADEPQGTLRDVPGRQAPAPKRTISRPTPPPSKKPQK
                     TTGSGGILAGIKNWLGKYF"
BASE COUNT          883 a          854 c          795 g          877 t
ORIGIN      
        1 gaatacaccg gcatccgaaa tcccgttccc atcccagtcc ccaatcaccg ggacatctcc
       61 ggttgtcccg aaatggaatg ccttgtcaac cacgccggtt tttgttgttt caagggccca
      121 gttaccggtg gacggacgga atacaccggc atccgaaatc ccgttcccat cccagtcccc
      181 aatcaccggg acatctccgg ttgtcccgaa atggaatgtt ttgtttactg ttccagtctt
      241 tgtcgtatcc aggtaccagt tcccgtttgc agggcggaat acaccagtat ccgaaatccc
      301 gttcccgtcc cagtctccaa tcaccgggac atctccggtt gtcccgaaat ggaatgtctt
      361 gtttactgtt ccggtcctgg tcgtatccag gtaccagttt ccgttggatg gccggaagac
      421 acctgcatct gaaatcccgt tcccatccca gtctccaatc actgggatat ctccggttgt
      481 cccgaaatgg aatgtcttgt ttactgttcc ggtcctggtc gtatccaggt accagtttcc
      541 attccggaat acgccggtgt gatgtgatgt gccgttgact atcgggttta cccgggcttg
      601 tccggagtcc tggttgatca ccagcgaact gcaatccact gcttcaaaat ctgatccctt
      661 cagctggtga agggcatgaa gggcatcgtt atcccagcga tcatccgggg caccggtaat
      721 ataccatggc gcaccattat cagacaggat catcccatat tttttcagcg ccagcagtac
      781 aatttgtgcc tgataagggt accctgaggt attgaaagat gatttaagcc tgaatctctg
      841 acccatcggc ggatacgacg ggtccgtgtt cgatgaggca taatgacggg caggccatat
      901 atatgccttc cttgttgaag gggcggtgaa acggagtgcg tgggtaatct cgccagtact
      961 gacttcatca tagcgggcaa gaccgggaag aacggcaaga cctgccgcat ctgctgaagt
     1021 ccagccttgg ggtcgcagag tatagccaga taaattaaaa atcgctcctg aaccggcgga
     1081 ccatgatccg tcgtcctgct tctccgcggc gaaaagttca tagagcatct ttgtgtccct
     1141 gtcaataatc agcagatgat ggtccgaccc actctcgatt aacgggttgt ccggaacagg
     1201 atagggaccc ggatcacttt catctccata ttcaaaagag acggttcttt ttgcctgtga
     1261 cccattgacg atattgtagg ggatcccgat caggttgcct tcatataaac ctgagccgaa
     1321 gtccgcatgg aggtaggaat tgtttccgat agaactggta tacgctgagg attgggaatc
     1381 aaggggaagt gaatcaacac gggtattcca gatatggtcg gcaggaaaga tcaaaactcc
     1441 gctgactgat ggtccgctga caaggacctg cggtgtttcc ctaccagcag gaccaggagt
     1501 ggaactatca atatagcgga atgcactatc atttttaaga ccatcggtat tgtgaatcat
     1561 gataccagtc gcactagcga agggttccat aaccatgagg gaaaatacag aaatcacgac
     1621 gattcttatc cagaggttca taagtaaaat cctcttcatt ctggtttatc tgccaaaatc
     1681 ttctctgagc tggtatataa attaaccata acccgatatt gggcagctac aacctgatgg
     1741 ttcaggaccc aataccagcg tcatataaat aaaacggata aaaaacccgg ttcctgttga
     1801 attcctttga gtgctgagtc cctgattata cgtcagacca cgtcaagcga atcaaggcga
     1861 ttttttaatt ttaaaaatgc tctggaattt ctttttgtta aaacctttag gcttttcagg
     1921 gtgccttttt cctgaattca cttcatattt tatgagtata aactaccggc aggtttataa
     1981 ttaattgaaa gatctgaaaa gagagaggaa tttgtcaaaa gatatcgtga gcgtcagtga
     2041 tgaccagacc gtggcaaccg tagcaggcat tatgggggaa gaacgggttg ggagtgtaat
     2101 tgtggatggt aagggaaagc catcggggat ttttaccgtg cgggagacct cctctcaaaa
     2161 ttccttattg cgggcaaatc ccttgatgag ccggtcggta acgcatcctc atctccgtta
     2221 aaaacagcag aggcggggat cagcatccat aaagcggcaa agattatggc tgcacagcat
     2281 gtgaggcgac ttcccctgat gaaaaagaag ctcatcgccg ggattattac cgcacgggac
     2341 cttatcgagg cctacgcacg gtaacaaaga aacatcccgt tcccgcagtt ttcagatacc
     2401 ttctccagag aatcccggat gcagttcttt accggatggg cttcatgagt aaaaatgatg
     2461 aatgtgatct cagcttgaac gccaccgctt cacaacgatg tcatcacccc tcttttttat
     2521 ctccaccttg atctttccat ctttatagtc cacctcaaag atccccatct tttttgcatt
     2581 gacaagctgc tgcagtttca gcatatcctt cctcatcatc cgtttaaaat cgtcaccctc
     2641 atagatcacg ctggccatat ttttttcacc cttacctgtc aaatgtgaat tgtccacaat
     2701 acgtatcaat gtagcgtgat tttcatgatt tgacgtattc ctcgaatcag gatgccaatt
     2761 aaccgaagca ttaccgtttt tttaagagaa gcatttaata tcgtatccct gctaacccta
     2821 tatgaggagt tatgagcagg gagaagatca aagtccccgg aaaggtatac gaggagttag
     2881 tcgccctcca gcgtgagatc catttcacgc aggatcacca ggacaccatc aagaaggcgg
     2941 aagaccgcgg gtatatgaac gcctccaact ggatccgcca gaacgagaat gcttacaaga
     3001 tcggcttcgc ctggggtttt gaaccaatgg cagatgagcc gcagggaact ctccgggacg
     3061 tcccgggacg ccaggccccg gcacccaagc gaacaatttc ccgtcctacc ccacccccaa
     3121 gtaaaaagcc gcagaagacc acgggttccg ggggtatcct ggccggtatt aaaaactggc
     3181 tcggcaaata tttctgatta tttttttttc tttttttttt taaagcaaaa ctactatttc
     3241 ctccaataaa aaagaagaat gtatgccaac ctgcagtgac tgtgtaagtt ataccccaat
     3301 aaccccgggt cggggcgatt gccggatcaa tggtgagaca cccccggacc gtgagagtga
     3361 acgatgccct tcacgtacgt ttattccaaa gccggtgaaa taatgctgt
//
LOCUS       DASRLV010000173         8040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_154651,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000173 DASRLV010000000
VERSION     DASRLV010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 8040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8040
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154651"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /locus_tag="VFC43_06760"
     CDS             complement(<1..1379)
                     /locus_tag="VFC43_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012108036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZK30916.1"
                     /translation="MLILDLDIPHNLKERYAQAGISELYPPQAECVEKGILAGKNTLV
                     AIPTASGKTLIAEMAMHNAIKAGGKCLYIVPLKALAGEKYEEFSNKGVKTGIATGDFD
                     RRDVLLGRNDIIVATSEKVDSLLRNNAPWISNITLLVIDEVHLIDSPGRGPTLEMVIA
                     KMRFRNPAMQVIGLSATIGNPDVLARWLDAELLTSTWRPVDLRSGVFFKDCIHFRSGI
                     RSVKVVSKNYDDLNLCLDTIDEGGQCLVFVSSRRNAEAFAKRAAGAIKSNDPGLAVYA
                     ERIQKGAETDMGKLLASCVAKGAAFHHAGLGRPERSIVEEGFRKGLIKCISSTPTLAA
                     GLNLPARRVIIRDYHRFVAGEGMQPIPVSEYHQMAGRAGRPRLDPYGEAVLIAKDQGQ
                     VEELFEWYIEAPPEEVHSKIADPTALYTHILSLIASGFAKNRNELTSFMNRTYYVHEH
                     RQGRLMQRAV"
     gene            complement(1520..2065)
                     /locus_tag="VFC43_06765"
     CDS             complement(1520..2065)
                     /locus_tag="VFC43_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HZK30917.1"
                     /translation="MMQEVKIAGSRVGVLIGKGGATKKELETKTHTTITVDSKEGLVK
                     VESSEEHTVALLRAVEIINAINRGFSPERAFEMIEDEDLLLDVIDLSGMAEGPRQLDR
                     LRGRIIGKDGRAREQIEDMTDVEISVFGKTISLIGYPEQLKTARAAIDMLIEGVPHEN
                     VFAFLDRKKKEAKQDMISYYY"
     gene            complement(2146..2904)
                     /locus_tag="VFC43_06770"
     CDS             complement(2146..2904)
                     /locus_tag="VFC43_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase RIO"
                     /protein_id="HZK30918.1"
                     /translation="MDQFDKKLEEMGVRIKDADQFKVSDDVFDDVTLLALYKLVHKKH
                     LSVIGGSISTGKEANVFYGERDGTSIAIKIYRIRTANFTTMSSYIVGDRRFSRVKKSR
                     KELIFAWTRKEFSNLARAREAGIPVPEPLVWDRNILIMSFLGENEIPYPHLKNSHLDD
                     PAEIYATIMNYIDLLYKKAELVHGDLSEFNILYGDQPYLIDMGQSVTRDHPRAYQFLM
                     RDIRNINRFFKNRCDVRNDVEIFNAITGLDTAEP"
     gene            complement(2958..3899)
                     /locus_tag="VFC43_06775"
     CDS             complement(2958..3899)
                     /locus_tag="VFC43_06775"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HZK30919.1"
                     /translation="MDTYERVTRNTVEVVTEDDLRTLLAQPQKKVYAGYEPSGEIHLG
                     HLVTINKLVDLQEAGFEITVLLADLHAFLNRKGTMEKVGELAEYNKRCFEGLGLKNVK
                     YVLGSDLQLNRDYELLVLQLSQQITLNRATRSMDEVGRQMDHPTVSQMVYPIMQMADI
                     AMLGVDAAVGGIDQRKIHMLAREHLINFGYKAPVCIHTPILNGLDGKKMSSSQGNYIS
                     VADTEEEILKKCQKAFCPPEIPENPVLQIFQYHIFPRLPTITIKRPEKFGGDRTFSEY
                     KELELSYGNGEVHPLDLKKSCGLSLVEILEPVRNYIG"
     gene            complement(3925..4956)
                     /locus_tag="VFC43_06780"
     CDS             complement(3925..4956)
                     /locus_tag="VFC43_06780"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012108032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HZK30920.1"
                     /translation="MIKVAINGYGTIGKRVADAVAAQKDMKVIGVSKTRPNAEAFVAK
                     QRGYPLYIADISKKPAFDKAGLNVVGSVEDMCKAADVIVDATPGDVGATNKPLYEKLG
                     KKALWQGGEDHDVAGFSFNSSCNYKDAIGRQFVRVVSCNTTGLCRIIHEIDKEFGVSH
                     VHAIMVRRGSDPGEIKKGPIDAVVLDPVTVPSHHGPDVLTVLPHISITTMAMIVPTTM
                     MHMHAIQMTTKKPVSRERVIDLIKNHPRLGLIKKTLGIKSTAELKEFAMDLGRQRSDL
                     WENCIFEESIYANKNELCFFQAIHQEADVVVENIDAIRAMVGHEKDAQKSIMATNEAL
                     GFSLIQNNH"
     gene            complement(5065..6807)
                     /locus_tag="VFC43_06785"
     CDS             complement(5065..6807)
                     /locus_tag="VFC43_06785"
                     /inference="COORDINATES: protein motif:HMM:NF024818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZK30921.1"
                     /translation="MSGLEAVSVLCVDDEALLLEVFEDFFKREPGFSITTCANATEAL
                     EKFSAQSFDAILADYGLPDMDGINLLKEIRAGGFSGLFIIITGKHRAHVAIDALNNGA
                     DYYVQKGGGMVNDMAALVEFIREGVGKIRANQDVAKRESLYRSIVENNSDLLCSFLKE
                     GEIRFVNNSYANFVGKSKKECIGSNFYSFVPAAESKNIGEQLHNLVPSKPDIILEHPL
                     NKSEGTSPIIQWNYRALFNQDGEIIEYQTAGREQSGFVRSAEPHVSKIEGTLAISAPA
                     AMTGAEPEDDWRTFSHTIDNLESPVFAIDKTGVIITWNKAMEQLTGFSAKEMVGKGKR
                     EYAIPFYGEPRPMLVDYIVISTDKRQSGKFPGIKRSGNTFTSEMEKVKIRDKPMCLWG
                     KGTAVYDSKGGLIAAIETITVVEQQHDALKQGELEKETYIGGLSSPTLKVAGGSASGA
                     IAGLIPSPPRGYGIYATNKRLFVIQNPDFDLSKPKGMQYGPYVLDDLFGTRTTIDSRP
                     KTIDTIEKLLVYEIAKQDLVTIELMNPVLLPGFLTMYNKTGESFRMFIDHKTAFLQIE
                     HLIKTFYPEILRLE"
     gene            complement(6983..7474)
                     /locus_tag="VFC43_06790"
     CDS             complement(6983..7474)
                     /locus_tag="VFC43_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF367 family protein"
                     /protein_id="HZK30922.1"
                     /translation="MIPLYAFRDNTCDPRKCTVKKLEKSGFLKIFTKISQIPRNTLLL
                     DPTAEQALSPADRYVKSITVLDCSWVVLDTGKVRSWRIRRALPFLIAANPVNFGKPCV
                     LSSVEALAGAVYILGEKDKAADLLSKVSWGIRFLEVNKEPLELYSNARDSTEVVKIQA
                     LFL"
     gene            7505..>8040
                     /locus_tag="VFC43_06795"
     CDS             7505..>8040
                     /locus_tag="VFC43_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HZK30923.1"
                     /translation="MYVLCSPCILNPALRAKGITKSSDLELFRHAINRCKKFGIEMVP
                     LPCPETVYLGPDRKPGTFLERLNTPEFPNLLDGLEEKTKAIIAKKGNPVCILGVNSSP
                     TCGVTSMYYGSEGTTPPKRHGRGVFLKGFPDIVAMDVTTFSRYQVYLAAPLFSEAERS
                     YNRSIEELLRKNFFDVYLP"
BASE COUNT         2097 a         1905 c         1661 g         2377 t
ORIGIN      
        1 accgctctct gcataaggcg cccctgtcgg tgttcgtgga catagtatgt cctgttcata
       61 aacgatgtga gctcgttacg atttttcgca aaaccggagg ctattaatga taatatatga
      121 gtatagagtg ccgtgggatc ggcgattttg gagtggacct cttcaggagg tgcttcgata
      181 taccattcaa aaagctcttc cacctgaccc tgatcttttg cgataagaac cgcctcccca
      241 tagggatcaa gtcgtggacg accggctctt cctgccatct ggtgatattc actcacggga
      301 attggctgca tcccttcccc cgcaacgaat cgatgataat cccttataat cacccgtcgt
      361 gcgggaaggt taagcccggc agcaagagtt ggtgtagaag aaatacattt gatcagaccc
      421 tttcggaatc cctcttccac aattgaacgc tcaggccgtc ctaaaccggc atggtgaaat
      481 gcggccccct ttgccacgca ggatgcaaga agcttaccca tatccgtttc agcacctttc
      541 tgtattcttt cggcatatac ggcgagtcca ggatcgttac ttttaatcgc accggcagct
      601 cgttttgcaa acgcctcggc attacggcgt gatgatacaa aaacaaggca ttgtccgcct
      661 tcgtcaatcg tgtcaagaca gagattgaga tcatcgtagt tttttgaaac cactttgact
      721 gatcgtatcc cgctcctgaa atggatgcaa tccttgaaaa aaacacctga ccggagatca
      781 acagggcgcc atgtgctggt tagaagttct gcatcgagcc atctcgcaag cacatccggg
      841 tttccgatgg ttgcagaaag tccgattacc tgcatcgcag gattcctgaa acgcattttt
      901 gcaataacca tctcaagtgt cggaccccga cccggtgagt caatcaggtg aacttcatcg
      961 ataacaagga gggtgatatt cgaaatccat ggtgcattgt tacgtaaaag agaatccact
     1021 ttttcactgg tagcgactat gatatcgttt ctccccaaca acacatcgcg acggtcaaaa
     1081 tcaccagttg caatgccagt tttcactcct ttgttactga attcttcata tttttcacct
     1141 gcaagtgctt taagagggac tatataaaga cattttcccc ccgctttaat ggcattatgc
     1201 attgccattt cggcaatcag agtctttccg cttgctgtcg gaattgcaac gagtgtgttt
     1261 ttcccggcaa gtattccttt ctcaacacac tcagcctggg gtgggtataa ctccgaaata
     1321 cccgcctgtg catatctctc tttcagatta tgggggatat ccaggtccag aattagcatg
     1381 attaatcctt ttaaaatatc cctgtccaat actcaattat catctttttt taaatttatt
     1441 ccatgataat gattgatttt ccgatatcaa caggatttat ataaatagtg gaatgggaga
     1501 atcaggaaat atttccccat tagtaataat aactgatcat atcctgtttg gcctctttct
     1561 ttttcctgtc aaggaatgca aatacatttt cgtgggggac accttcaatc agcatatcga
     1621 tcgctgcacg ggcagttttt aattgctcgg gataaccgat cagggagatt gtcttgccaa
     1681 agactgaaat ctccacatca gtcatatcct caatctgctc tcgcgcacga ccatcttttc
     1741 caataatcct tcctctgagt ctgtccagct gccgcggacc ctctgccatt cctgagagat
     1801 caatgacatc aagaagcaga tcttcgtcct cgatcatttc aaaagcacgt tccggtgaaa
     1861 atccccggtt gatggcattg ataatttcca ctgcacgcag caaggctacc gtgtgctcct
     1921 ccgaactctc cacctttaca agaccctctt tactatcaac ggtaattgtt gtgtgagtct
     1981 tcgtttcaag ttcttttttt gttgcaccgc cttttccaat cagcacgcct actctgcttc
     2041 cggcgatttt tacttcctgc atcatgttag tttcacctcg aatatatgat gtttgttcat
     2101 ttcacggaac ttttttagta tatagtataa cgcgttcccc agttatcatg gttcagctgt
     2161 atcaagtccg gttattgcgt taaagatttc gacatcgtta cgcacatcgc agcggttttt
     2221 gaaaaacctg tttatatttc gtatatcccg cattaaaaac tgataggccc ggggatgatc
     2281 acgagtaact gactgaccca tatcaatgag ataaggttgg tcaccatata atatattgaa
     2341 ctcactcagg tctccatgga cgagttcagc ttttttatag aggagatcga tatagttcat
     2401 gatggtggcg tagatttccg caggatcatc aagatgagaa tttttaagat gagggtatgg
     2461 aatttcattc tctcctaaaa acgacatgat cagaatattc ctgtcccata cgagtggctc
     2521 gggtacggga atacctgctt cccgtgctcg tgcaaggttc gaaaactcct tccttgtcca
     2581 ggcaaaaatc agttctttac gggatttttt cacccgggaa aatctccggt cgccaacgat
     2641 atatgagctc atcgttgtaa agtttgcagt acgaatgcga tagatcttta tcgcaatact
     2701 ggtcccgtca cgttcaccat aaaatacatt cgcttctttg cctgtgctta tcgatccgcc
     2761 aattactgac aggtgttttt tatgtacgag tttgtagagc gcaaggagtg tgacgtcatc
     2821 aaaaacatca tcagaaactt taaactggtc cgcatctttg atacggacgc ccatctcttc
     2881 aagtttctta tcgaactgat ccattcgttt ttctacactc acagcgggcc accaacaatt
     2941 tttagataat ttctttacta ccctatatag ttccgcaccg gttcaagtat ttctacaagt
     3001 gacaacccac aggatttctt gagatcgagt ggatgcactt caccgttgcc ataggataat
     3061 tcaagctcct tatactcaga aaaagtccgg tctcctccga atttttccgg tcttttgata
     3121 gtaatcgttg gcagacgggg gaaaatatga tactggaata tctgaagaac cggattctcc
     3181 ggaatttcag gagggcaaaa ggccttctgg cactttttta aaatctcttc ctcagtgtca
     3241 gcaaccgata tgtaatttcc ctgagaggaa gacatctttt ttccatcgag cccattgagg
     3301 atcggcgtgt ggatacaaac aggtgctttg taaccgaaat taatgaggtg ttcacgggcg
     3361 agcatatgga tcttccgttg atcgatacca ccaaccgcgg catcaacacc gagcatcgcg
     3421 atatctgcca tctgcataat ggggtaaacc atctgagata cagtcggatg atccatctgc
     3481 ctccctacct catccatgct tcgtgtggcc cggtttaagg taatctgctg ggaaagctga
     3541 agtacaagca gttcatagtc ccggttcagc tggagatcag atccaaggac atatttcaca
     3601 ttcttcaacc cgagtccctc aaaacaacgc ttgttgtatt cggcaagctc gccgactttt
     3661 tccatggttc ctttacggtt gaggaaagca tggagatcag ccagcaaaac agtgatttca
     3721 aaacccgctt cctgaaggtc taccagtttg tttatcgtga caagatggcc gagatggatc
     3781 tcaccactgg gttcataccc ggcatacact tttttttgcg gttgggcgag aagagtgcga
     3841 aggtcgtcct cagtgacaac ttcaacagta ttccgggtta cccgctcgta tgtatccatt
     3901 aaaaataatg ggttatgatt ggtgttaatg gttattctga atcaggctga atccgagcgc
     3961 ttcattggtc gccataattg atttttgcgc gtctttctca tgccccacca ttgcgcggat
     4021 tgcatcgata ttttcaacga cgacatctgc ttcctggtgg attgcctgga agaagcagag
     4081 ctcgtttttg ttggcgtaga tcgactcttc aaagatacaa ttttcccaca gatcagaacg
     4141 ttgccggcca agatccattg caaattcctt gagttcggcc gtacttttaa ttcccaatgt
     4201 ctttttaatg agaccgaggc gcgggtgatt cttgatcaga tcaattactc tctcccggct
     4261 taccggcttt ttggtggtca tctggatagc gtgcatgtgc atcattgttg taggcacgat
     4321 cattgccatt gtcgtgatgg aaatatgggg gagtacggtg agtacatccg gaccatggtg
     4381 actggggacg gtgactggat ccagtacaac agcgtctatc ggcccttttt tgatctcgcc
     4441 gggatcagat ccacgccgta ccatgattgc atggacatgc gatacaccaa actctttatc
     4501 gatttcatgg ataatccggc aaagtccggt tgtattacac gaaaccactc tcacgaattg
     4561 tctgccaatg gcgtctttat aattgcatga tgaattgaag gagaaacccg ccacatcatg
     4621 atcttcacct ccctgccaga gcgccttttt ccccagtttc tcatagaggg gtttatttgt
     4681 tgctcctacg tcacctggtg ttgcatcgac aataacatcg gctgctttac acatgtcttc
     4741 gacagagccg acaacgttaa ggccggcttt gtcaaatgcg ggtttttttg atatatctgc
     4801 gatgtagagt gggtaacctc tttgtttagc tacgaacgct tcagcgttcg ggcgggtctt
     4861 tgatacaccg atcactttca tgtccttctg ggcggcaaca gcatcagcaa cgcgtttacc
     4921 gattgtaccg taaccattga tggcaacctt gatcatacaa tcgtacatgg tagtagtata
     4981 caaaaaagct atgggggagc aacaatctgc ggatcctatg aaaagttatt cccacagaaa
     5041 aggataaaac ccactattta tcctttactc aagccgtaaa atctccggat agaacgtttt
     5101 tatcagatgc tcgatctgta aaaatgccgt tttgtgatcg ataaacattc tgaacgactc
     5161 ccctgttttg ttatacattg tcaggaaacc cggaagcaga accggattca ttagttctat
     5221 tgttaccagg tcctgttttg cgatttcata aaccagcaat ttctcgatag tatcaatagt
     5281 ctttggacgg ctatcgattg tagtcctggt accaaaaaga tcatccagaa catagggacc
     5341 atactgcatt ccctttggtt tggaaaggtc aaaatcagga ttttggatca caaacaagcg
     5401 tttgtttgtc gcatagatcc catagcccct tggtggtgaa gggattaagc cggcaattgc
     5461 cccgcttgcg cttccccccg caactttcag cgttggactt gataacccac cgatatatgt
     5521 ctctttttcc aactcacctt gttttagggc atcatgttgt tgttcaacga cagttattgt
     5581 ctcaatagct gcaatcaacc cacccttgct gtcatacaca gcagttccct ttccccacaa
     5641 acacatcggt ttatcgcgaa ttttgacttt ttccatttca ctggtaaatg tatttcccga
     5701 tctttttatt cccgggaatt ttccggattg tcttttatcc gtcgatatga ctatataatc
     5761 aacaagcatc ggtcttggct cgccataaaa tggaatcgca tactcccgtt ttccttttcc
     5821 taccatttct ttagctgaaa agccggtcag ttgttccatg gccttattcc atgtaatgat
     5881 aaccccggtt ttatcgattg caaaaaccgg actctcgagg ttgtcgatgg tatgggaaaa
     5941 tgttctccag tcatcttcag gctcagcacc tgtcatagct gccggagctg agatggctaa
     6001 cgtaccttca atctttgata cgtgaggttc ggcactccga acgaatccag actgctctct
     6061 tcctgcggtt tggtattcga taatttcgcc atcctgattg aaaagcgcac gataattcca
     6121 ttgaattatt ggtgatgtgc cctcactctt attaagagga tgttcaagga taatatcggg
     6181 ttttgaaggc acgaggttgt gtaactgctc cccgatattt ttactttctg cagctggtac
     6241 aaaagaatag aagttgctcc ctatacactc ttttttactt ttaccaacaa aatttgcata
     6301 tgaattattg acaaaccgga tctctccttc ttttaagaaa ctgcaaagaa gatcggaatt
     6361 gttttcgacg atcgaacggt acaggctctc tcgttttgcc acatcctggt ttgcccgaat
     6421 ttttccaaca ccctctctga tgaattcaac aagagctgcc atgtcattta ccattccccc
     6481 tcctttttgc acataataat ctgcgccatt atttagtgca tcaatggcaa catgagcacg
     6541 gtgtttaccg gtgatgatga taaatagccc tgaaaacccg ccggcccgga tctcttttaa
     6601 taaattaata ccgtccatat ccggaagacc gtaatctgcc agaatagcat caaatgattg
     6661 agctgaaaat ttctccaatg cttcagttgc atttgcgcat gttgttatcg aaaaacctgg
     6721 ttcccgtttg aaaaaatcct caaaaacctc cagtaataac gcctcatcat ccacgcacaa
     6781 aacagaaact gcctcaagac cagacaaagt aattaatcac tcctcatttg ctaccccggt
     6841 acctgatatt acatttactt tattacaggt attctataat attttagtcc aaggaatttt
     6901 caaagaatta ggcaaaaaac agtaattacc tattttttaa agattttgaa aataataaga
     6961 ttttttgtaa gaaaaaactc ccttaaagaa agagtgcctg gatttttaca acttcagtgc
     7021 tatcccttgc atttgaatag agttcaagtg gctctttgtt tacttccaaa aaacggatac
     7081 cccagctgac ttttgagaga aggtctgcag ctttgtcttt ttcaccaaga atatagactg
     7141 caccagccag tgcctcaaca gaggaaagta cacacggctt gccaaaatta acgggatttg
     7201 ccgcaattaa aaagggaagt gcccggcgga ttcgccatga ccggactttt cctgtgtcca
     7261 aaactaccca cgagcaatcc agaacagtta tagatttaac gtacctgtct gcaggtgaga
     7321 gtgcctgttc agctgttgga tcaaggagca aggtatttcg tggaatttgc gaaattttgg
     7381 taaaaatttt taaaaaacca gatttttcaa gtttttttac cgtacacttt cgagggtcac
     7441 acgtgttatc ccgaaatgca tacaacggta tcattgatca tctgtgcgtg cacacaaggt
     7501 atcaatgtac gttctttgca gcccctgcat tcttaatcct gcactgcgtg caaaagggat
     7561 tacgaaatcc tcggatctcg agctgttcag gcatgcaata aaccggtgta aaaaatttgg
     7621 gatcgaaatg gtccctttgc cgtgtccgga aactgtatat cttggacccg acagaaaacc
     7681 cgggacgttt ctcgaacgac ttaatactcc ggaatttcca aaccttcttg atggcctgga
     7741 ggaaaagaca aaagcgataa ttgctaagaa agggaatccg gtctgtatcc tcggggttaa
     7801 ttcatctcca acctgcgggg tgacaagcat gtattatggg agtgaaggta caacccctcc
     7861 taaacgacac ggacgaggtg ttttcctgaa gggtttcccc gatatcgtgg cgatggatgt
     7921 tacgactttt tcccggtatc aggtttatct ggcagccccg ctcttttcag aagccgaacg
     7981 atcgtataat cgatcgattg aagaactact gagaaaaaat ttctttgatg tctatttacc
//
LOCUS       DASRLV010000174         6166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_791958,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000174 DASRLV010000000
VERSION     DASRLV010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 6166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6166
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791958"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1107)
                     /locus_tag="VFC43_06800"
     CDS             complement(115..1107)
                     /locus_tag="VFC43_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZK30924.1"
                     /translation="MSLSKGCVLCYQGAKMVLFVTGRCDRSCWYCPLSLERKGSDTVY
                     ANEHPVDVPAQLIGEAENMSALGTGITGGEPLLCLDRVVLYCRLLKEHFGPEHHIHLY
                     TAKAPTDDELAKMKGLVDEIRLHPPHECWDTILDTEYIRSAKRAKEMGFDIGIEVPAL
                     PGLEHLIPALPFLDFLNVNELEWGETNAYRMRERGYELADEVHNAIEGAKGWAEEICR
                     QDKVHWCSSAFKDSVQLRERLKRIAENTARPFDEITPDGTIVYGVLEPPLEQKILCTQ
                     FCEQEFEPGSFETCGDRIEMAWWLLEKNSPDLPGKKYVVERYPNQGMVVEVTPI"
     gene            complement(1249..2757)
                     /locus_tag="VFC43_06805"
     CDS             complement(1249..2757)
                     /locus_tag="VFC43_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZK30925.1"
                     /translation="MVERNLIPTVKYVAATKPAFLLSEIIPYLDDPLTVNELFASISP
                     ELSDMGLVAKKITGDYEITKIPTAQPYLLNREELERHQAFFTQKTLSAPLAQAIQKYI
                     TKKVGKDWNDPVIIERLRRAIVAQKDDYWKPSHKRSLQYIKGYSVLGYLAYHFPVYFF
                     QTEHLLARLVSEGLVKKNMTILDVGTGPGVVPLAVADFYSRLDGAQATVYSLERSEEH
                     IEAFTFLRDAVVPKGGRVSVKPPIKTDIRNIDSAKLPKKIDLVVFSNVLNELSDATME
                     SRADIVTHIAKNLAPDGSVLIVEPADEENATRMRSLTIALHKKGLSIFSPCSFIWGST
                     CTAPRCWSFETAHAIRLPHLMETLAHCEDSYRYVNTDIKYSFAVLRKDKRTRENFRLP
                     AGSKFLRLSKLHLHTGKRINVAGIKMSEELGDDRSHVFKLCDGTAKTPVYAVLPSYHI
                     TPENDLITSLPYGSMLELRNVLVRYNKAHDAYNLLVNRNTKIDSFHTGKSSG"
     gene            2887..3573
                     /locus_tag="VFC43_06810"
     CDS             2887..3573
                     /locus_tag="VFC43_06810"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HZK30926.1"
                     /translation="MCGRYSLIFIDDLGNRFRVFNPMIGSRSRFNIKPGNEMPVIIHK
                     EKNELVVMKWGLVPHWVKDMKSAQRPINARAESLQEKPSFTDLLTNRRCLVPASGFFE
                     WKKEGNKRIPFYFHLQKAPLFAIAGLYDQWADPEGNLLTTYTIITTAPNEVVAKIHNR
                     MPAVLLPGYEDRWLSKTSLDTNDIKEILAPFPSGEMSGYPVSSLVNSPDTDDERVIRP
                     VNTLSYDLTS"
     gene            3587..3844
                     /locus_tag="VFC43_06815"
     CDS             3587..3844
                     /locus_tag="VFC43_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30927.1"
                     /translation="MPGHCPGAANIRTPTLTIKKCPQCGAEVELFSNDISVQCSACGF
                     IVYNDIESCIRWCRNAKECVGEEMYRKLMEGKSPPARQQKK"
     gene            complement(3853..4752)
                     /gene="nadA"
                     /locus_tag="VFC43_06820"
     CDS             complement(3853..4752)
                     /gene="nadA"
                     /locus_tag="VFC43_06820"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HZK30928.1"
                     /translation="MSWLLDEIHRLKEERHAIILAHNYQPDEIQDIADITGDSLELSR
                     AAATMEGDVIVFCGVDFMAETAAIISPEKTVLLPAPDAGCPMAEMISADELKFAKSRH
                     PDAAVVCYVNTTAEVKAVSDICCTSSNAVKVVNSLKEDQVIFVPDRNLALYTQRFTKK
                     EILPWEGFCRVHDRITADHVRRARDLHPGAIVLVHPECRPEVIDLADHVASTSGIIRL
                     VCELPDEEFIIGTEIGILHRLKKECPTKNCYPLSSGAICTNMKKTDLTKVRQALIDLQ
                     PRITVPEEIANRARRAIERMLAV"
     gene            4916..5110
                     /locus_tag="VFC43_06825"
     CDS             4916..5110
                     /locus_tag="VFC43_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZK30929.1"
                     /translation="MKLIVSAGDCRIFDHSPATVEQILIEQGINPLDVIVSRNGRLVP
                     EDTVVEADDEILITRIAHGG"
     gene            5114..6049
                     /locus_tag="VFC43_06830"
     CDS             5114..6049
                     /locus_tag="VFC43_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00269 family protein"
                     /protein_id="HZK30930.1"
                     /translation="MNGDTAGSAIPVCSLCREPSAVFQKLKDRHLCGIHFTADIEQRV
                     TDTITTREMIAAGDRVAVALSGGKDSTALLILLSRLLPQWPDVRLVAITIDEGISGYR
                     EETIFSAEELTRRLGIEHHCISFYELFGDSLDTILKGRETQACSICGILRKKALVVGA
                     GRIGATKLATGHNLDDEAQSVLMNVLRGDLPRLIRNSGMDSSGRFIPRIKPLSLISEK
                     EIALYLMLNNAWNVLPECPYTRFALRREVRSLLSGFEYRHPGTMLHLIESKQKIERSC
                     KGSEVSEPLKTCRGCGDPSSGEFCQVCLIRQSRNR"
     gene            complement(6035..6157)
                     /locus_tag="VFC43_06835"
     CDS             complement(6035..6157)
                     /locus_tag="VFC43_06835"
                     /inference="COORDINATES: protein motif:HMM:NF014671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HZK30931.1"
                     /translation="MAEKTGVTIIGFMRSGKMNIYSWPQRISGMKPAPGPSAVT"
BASE COUNT         1469 a         1553 c         1587 g         1557 t
ORIGIN      
        1 gcatctttgc ataccggcgg ttgccgtcct ggatgatggc gatgtggttt ggcacatgac
       61 ggtactgcat ctttacgtat ctctcgtaga gcgggtcaat cagcgaacgc aggatcatat
      121 cggtgtaacc tccacaacca taccctggtt gggatacctc tcaacaacgt attttttccc
      181 cggcaggtcc ggggaatttt tctcaagcag ccaccacgcc atctcgatac ggtcgccaca
      241 ggtctcaaaa cttcccggct caaattcctg ctcgcagaat tgggtacaga gtattttctg
      301 ttcaagcggt ggctcaagta caccgtatac gattgtcccg tcgggtgtaa tctcgtcaaa
      361 gggacgggcg gtattttctg cgatcctctt gagccgttcc ctcagctgca ccgagtcctt
      421 gaatgcagat gaacaccagt gtaccttgtc ctgccggcat atttcctcag cccagccctt
      481 cgcaccctcg attgcgttgt ggacttcgtc ggcaagctca taccccctct cgcgcatccg
      541 gtatgcattt gtttcacccc attcgagttc attgacattg aggaagtcca ggaaggggag
      601 tgcaggtatg aggtgttcga gtccgggtaa tgcagggact tcgataccga tatcaaatcc
      661 catctctttt gcccgttttg cagaacggat gtattcagta tcaagaatgg tatcccagca
      721 ttcatgtggc gggtgaagcc ggatttcatc aacgagccct ttcatcttcg cgagttcatc
      781 gtcagtaggg gcttttgcag tatacaggtg tatatggtgt tcgggaccga aatgttcctt
      841 gagcaaccgg caatagagaa cgaccctgtc aagacacagg aggggttccc cgccggtaat
      901 cccggtaccg agagcgctca tgttctctgc ttccccgatg agctgggcgg gcacgtcaac
      961 cgggtgctca tttgcataca ccgtgtcgga ccctttccgt tccaatgaga gcgggcagta
     1021 ccaacaggag cggtcgcagc gtcccgtaac aaagaggacc atctttgcgc cctggtaaca
     1081 gagaacacag ccttttgaaa gtgacactca gactccctgc ctgtacgggg acttcacgtt
     1141 agattccgtt cttatttttt tagtgagcaa agcacaaaac tcttctgagt acatacctga
     1201 gaaaaacgag ataatcggac catcagtttt caaattcctg attttgagtt acccgcttga
     1261 ttttcctgtg tgaaatgaat caatcttcgt attacggttc accagcaggt tatatgcatc
     1321 atgtgctttg ttgtaccgca ccagtacatt tcgcagttca agcatcgatc catagggtag
     1381 tgaggtgatc aggtcatttt ccggtgtaat atgataggaa ggcagcacgg catatacggg
     1441 tgttttcgcg gtcccgtcgc agagcttgaa cacatgagac cggtcatctc ccagctcttc
     1501 agacatcttt atcccggcaa cattgatccg tttcccggta tgcaggtgca gtttggataa
     1561 ccgcaggaat ttcgatccgg ccggcaaccg gaaattctcc ctggtccttt tatcctttcg
     1621 caggaccgca aaactgtact tgatatcggt attgacgtac cggtatgagt cctcgcaatg
     1681 ggccagggtt tccatcaggt gaggcagcct gatggcatgt gccgtttcaa aactccagca
     1741 gcggggggcg gtgcaggttg aaccccagat aaatgaacag gggctgaaga ttgacaggcc
     1801 tttcttgtgc agtgcgatag tgagggatcg catccgtgtc gcattctcct catccgctgg
     1861 ctctacgata aggactgaac catcaggtgc gaggtttttt gcgatatggg ttacgatatc
     1921 agctcttgac tccatggttg cgtccgaaag ctcattcagc acattggaaa agaccacgag
     1981 atcaattttt ttgggaagtt tcgcactatc gatattccgg atgtctgttt ttatcggggg
     2041 cttgactgac accctgcccc ctttgggtac gacagcatcg cgtaaaaagg taaacgcctc
     2101 gatatgttct tctgagcgct caagtgagta gaccgtggcc tgtgcaccat cgaggcggga
     2161 ataaaaatcc gcaacagcaa gcgggacaac gccgggccct gtcccgacat caagaatcgt
     2221 catgtttttt ttcaccagcc cttcagagac gagcctggca agcaggtgtt cagtctggaa
     2281 aaaatatacc gggaagtggt aggcaaggta tccgagcacg ctgtagcctt ttatgtactg
     2341 cagcgaccgc ttatgggacg gtttccagta atcgtccttc tgtgccacaa ttgcacggcg
     2401 gagccgttcg ataataaccg gatcgttcca gtctttcccg acttttttgg tgatgtattt
     2461 ctgaatggcc tgtgcaagcg gtgcagacag tgttttttga gtgaaaaacg cctggtggcg
     2521 ttcaagttcc tcccggttta aaagatatgg ctgcgcagtc gggatttttg taatctcgta
     2581 atctcccgtg attttttttg ccacgagccc catatcggaa agttcaggag atatactggc
     2641 gaacagctca ttcaccgtta gcgggtcatc gaggtaaggg atgatctctg ataagagaaa
     2701 agcaggtttt gttgcggcca catactttac cgtcgggata aggttacgtt cgaccattag
     2761 ttgccagtta cttggcgatg ggcgatatta catctttgta tcgtacggga tatgagtgat
     2821 tatttttttg ttacctgcgg caggatgata tacccgtcaa gcgtacatcc gaaacgataa
     2881 ggcattatgt gcggcaggta ttcgctcatc tttattgatg atctgggcaa caggttccgg
     2941 gtattcaacc ccatgatcgg gtcacgctca cggttcaata tcaaaccggg gaacgaaatg
     3001 ccggtgatta tccataaaga aaaaaatgaa ctcgtcgtga tgaaatgggg gctggtcccc
     3061 cactgggtaa aggatatgaa atccgcacaa cgacccatca atgcacgagc agaaagcctg
     3121 caggaaaaac cctcttttac cgacctcctc acaaaccgcc gctgtcttgt cccggcatca
     3181 ggcttttttg aatggaaaaa agaaggcaac aagaggatcc cattttattt tcacctgcaa
     3241 aaggcaccgc tttttgcaat tgccgggctc tatgatcaat gggcagaccc tgagggaaat
     3301 ctccttacta cctacacgat aataaccacc gcacccaacg aggttgtcgc aaaaatccat
     3361 aaccgtatgc cggctgtctt gttaccggga tacgaagatc gctggctttc aaaaacctcc
     3421 ctggacacaa acgatatcaa agaaattctt gccccgtttc cctcagggga aatgtcgggt
     3481 tatccggttt cttctcttgt caattctccg gatactgatg acgagcgggt tatccgcccg
     3541 gtcaatactt tatcctatga tctgacatca taacagggac ataccaatgc ccggccattg
     3601 tccaggcgcc gcgaacataa ggactccaac actcacgatc aaaaaatgcc cgcaatgcgg
     3661 tgcggaagta gaattatttt caaacgatat ctcagtacaa tgcagcgcgt gcggttttat
     3721 cgtctacaat gatatcgaat cctgtatacg gtggtgccgg aacgcgaagg aatgtgttgg
     3781 tgaggaaatg tacaggaaac taatggaagg aaaaagtcct cctgcccggc agcagaagaa
     3841 gtgattccct cgtcagaccg caagcatcct ctcaattgcc ctgcgtgccc ggttcgcaat
     3901 ctcttctggc acggtgatgc gaggctgcag gtcaataagg gcctgccgga ctttcgtaag
     3961 gtcagtcttt ttcatgttcg tacagattgc tcccgaagat aacgggtagc agttctttgt
     4021 cgggcactct tttttgagcc ggtgcaggat gccaatttct gttccgatga tgaactcctc
     4081 gtctggcaac tcacaaacaa gcctgataat tcctgaagta cttgccacat ggtctgcaag
     4141 gtcaatcact tcagggcggc attcgggatg cacaaggact attgcaccgg gatggaggtc
     4201 gcgtgccctg cgcacatggt ccgccgtgat ccggtcatgc acgcgacaga atccctccca
     4261 gggaaggatc tctttttttg taaaccgctg ggtatagagt gcaaggttgc ggtcgggtac
     4321 aaaaatgacc tggtcttctt ttaatgagtt gaccaccttc accgcatttg atgaggtgca
     4381 gcagatatcg cttacggctt ttacttcggc tgtggtattc acgtaacaga caactgctgc
     4441 gtccgggtgc cgggattttg caaatttcag ttcatctgcc gagatcattt ccgccatcgg
     4501 gcaaccggca tcaggtgccg ggaggaggac ggttttttcc ggagagatga ttgcggcggt
     4561 ctcagccatg aagtccacac cgcagaacac gatgacatca ccttccatgg tggccgcggc
     4621 ccgggagagt tcgagtgaat ccccggttat gtcggcaata tcctgtattt cgtcgggctg
     4681 gtaattgtgc gcaagaataa tcgcgtgccg ctcctctttt aaccggtgaa tttcatctaa
     4741 aagccaagac acacgctgca ccctgcacac ttccgtactt gtatgtgttg atccactgta
     4801 tcaaatatga ggttgcagag aggcgttcaa catgtggttt acgaatgtac cgcttctcat
     4861 tttttttcat gcgggatctt taagaaacct gcccgccaac ctacaccatg acgaaatgaa
     4921 actcatcgtt tcagccgggg attgccggat ctttgatcac agcccggcaa ctgttgaaca
     4981 gatcctcatc gaacagggta ttaacccact ggacgtgatc gtatcccgga acggcagact
     5041 ggtacccgag gatacggttg tggaagcgga tgatgaaatc ctcattaccc gtatcgccca
     5101 cgggggttga cgggtgaacg gggatacagc aggatcagcc ataccggtct gttccctgtg
     5161 cagggaacct tcagcagtgt tccagaagct aaaagaccgt catttgtgcg gtattcattt
     5221 tacggctgat atagaacaga gggtcacaga tacgataaca acccgggaga tgattgccgc
     5281 tggcgaccgg gtagcagttg ccctgagcgg aggcaaggac agcacggcac ttctgattct
     5341 cctgagccgg cttctcccac aatggccgga tgtacgtctt gttgccatta cgatcgatga
     5401 gggaatttca ggctaccggg aagaaacaat tttctcggca gaggagctca ccaggagact
     5461 cgggattgaa caccactgca tctcctttta tgagctgttc ggggattcac ttgatacgat
     5521 cctgaaaggc agggaaacac aggcctgcag catctgcgga atcttgagga aaaaagcact
     5581 ggtggtcggg gcaggccgga tcggtgcaac gaagcttgcc acgggtcaca accttgacga
     5641 tgaagcccag tcagtgctga tgaacgtgtt gaggggggac ctcccgcggc ttatcaggaa
     5701 ttccggcatg gattcatcag gcaggtttat accgcggatc aaaccgctat ccctgatttc
     5761 agaaaaagag atcgccctgt acctgatgct caataatgca tggaacgtac ttcccgagtg
     5821 cccgtacacc cggtttgcct tacggcggga ggtccggtcg ttgctctccg ggtttgaata
     5881 caggcacccc ggcacgatgc tccatctgat tgaaagtaaa caaaagatag agcgttcctg
     5941 taagggatca gaggtttctg aacccctcaa aacctgccgc gggtgtggtg atccttccag
     6001 tggggagttc tgccaggtat gcctgatccg gcagtcacgt aaccgctgaa ggaccgggtg
     6061 cgggtttcat gccggaaatc cgctgtggcc aggaataaat attcattttt cctgaccgca
     6121 taaaaccaat gatagtaaca cctgtttttt cggctatttc aaccga
//
LOCUS       DASRLV010000175        12636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_389419,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000175 DASRLV010000000
VERSION     DASRLV010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 12636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12636
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389419"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /gene="xseA"
                     /locus_tag="VFC43_06840"
     CDS             <1..941
                     /gene="xseA"
                     /locus_tag="VFC43_06840"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HZK30932.1"
                     /translation="PHGSYQLQVKEMRKAGLGEKFLLVEQWKKQLAAEGCFSLERKRC
                     LPPFPARVGVVTSETGAVIHDIRTVVGRRYPLEIIISPTAVQGELAHREIAAAICRIS
                     GLVDVVIVARGGGSFEDLFPFNHPDVVRAIVACPVPVVSAIGHEVDVTLSDLAADLRA
                     ATPSAAAELVVPDRLVLTARLQELRSQLNAALQARVAWAWDEISDLRDRLRPQRFTRK
                     IEERKQNTADLSERLERAFSNRLGRERLLLAEMRTALDSRNPVAVLARGYCVAEKQGR
                     VVKSAGTIIKGDRMNIRFYDGSSQVTVEDVNHERDL"
     gene            925..1110
                     /gene="xseB"
                     /locus_tag="VFC43_06845"
     CDS             925..1110
                     /gene="xseB"
                     /locus_tag="VFC43_06845"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HZK30933.1"
                     /translation="MKETYEVKIEQLRKIIEKIEDGNTGLDESMKLYEQGAVLVKQCE
                     VLLTEAELKITTLSRDA"
     gene            complement(1094..1522)
                     /locus_tag="VFC43_06850"
     CDS             complement(1094..1522)
                     /locus_tag="VFC43_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF371 domain-containing protein"
                     /protein_id="HZK30934.1"
                     /translation="MEAQETIHCRGHPLVLGNHPTTFEVTAEGHLSKNGNCIIGISAD
                     KGCKGLSAGFRRVLCHDDAVLFTHLSCGGITAEVKSRGSSAFTLDHPTDMVWRRSTFV
                     CGRTIGILSDHVAADLPEALIANLVLGKEMTVTLTATRPG"
     gene            1556..2131
                     /locus_tag="VFC43_06855"
     CDS             1556..2131
                     /locus_tag="VFC43_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HVO_0476 family zinc finger protein"
                     /protein_id="HZK30935.1"
                     /translation="MFITVPCPECNEETEHEIITESHDLLVRCTTCGHHQRIKREKEP
                     QSILIKTIVSKEGTSLVCGIELAEDDECSVGDHLVAECGDEAFGVEVSSIECGEKRQN
                     RAKAPDITTLWTRTIEQVVVKISIHDGRKTAPVYMEVDGEQPFVVGEMYVASGKRFKV
                     SHLKLRDGPLMRKEGWKSVAHRIKRIYGTRL"
     gene            complement(2126..2386)
                     /locus_tag="VFC43_06860"
     CDS             complement(2126..2386)
                     /locus_tag="VFC43_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZK30936.1"
                     /translation="MAVRVICFYQPGCMGCMEQTPINAEVAKALNITIEEIDAIKNPH
                     FIKEFTLKVTPTIIILKEEKVLERFEGVVHPEQLRAAIIKYL"
     gene            complement(2432..3121)
                     /locus_tag="VFC43_06865"
     CDS             complement(2432..3121)
                     /locus_tag="VFC43_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30937.1"
                     /translation="MGEKKKKEETMKTVKKVLIVGACVLFVVLMVVSGMGSGWLTMFT
                     SVRPGDSVVVDYTIFDAAGQPIVTSEKATYDTVVGKNRQIFGSKQLTIISNQTIEKPI
                     YSVPVYPSNGASVKQLAIFSSEYNVITSAIIGMKVNEQKRIPIGSNISMTQDWSVEQL
                     AQSKINLTDINVGDNFFMGVSDNPEAVVNNTTATTFLRIGEVASKSPEGIVVDFSYPS
                     IDIRVLSINKQ"
     gene            3347..4132
                     /locus_tag="VFC43_06870"
     CDS             3347..4132
                     /locus_tag="VFC43_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZK30938.1"
                     /translation="MDKKKVKDYMTYDVVSIDFHGTVKDIIEKIQKTHHDGFPVVEGD
                     EVVGYVSARDLLFVQLTTPVKEVVSKSLIVADPEMDINDAARVIFRSGIQKLPVVDEK
                     NHLLGIISNSDVIRSQIEHVSPEKVFNFITTLKKLYGVDPTLDRGTVPVRDLLPTQSK
                     IYEDELEGRIYEIKKGLAEPIIVVKRPGRIILVDGHHRAIAAKRLGITAIDAYIIEIK
                     EDIELGMERTAHAMNLKSLDDVQVLDYARHPLVAITHRLVKRV"
     gene            complement(4606..5769)
                     /locus_tag="VFC43_06875"
     CDS             complement(4606..5769)
                     /locus_tag="VFC43_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HZK30939.1"
                     /translation="MCGGEGTRLRPLTFERPKPCIPIVNRPSIQHLVSHLSNMGFREV
                     VVTLGYMGSSIEEALGDGSLFGVEITYVHEKTKLGTAGSVKNAQRYLDGQDFLVVGGD
                     HVTDLNVLEFYRAHQKEKATTTIGLISIDDPGEYGIAEIDVTYEIKRFKEKPAPGEIF
                     SNLASTGMYVCNPAVFDHIPPATKYDFARDLFPQMMEENFVLKGWLARGNWTDVGSPH
                     SLRQAERWKLQDIAFTDIIGNLSMHGAQIQGPVQLGDSITLGKNTRVIGPVAIGPGTT
                     IGDNVLIGPYTSIGEKCILRNNTKVFSSSLYNRVIVGANTTISGCIMDNDTHIGEHCS
                     IENDTVIGPRVVLRDRVVVHSKTRLWPEVVVSDGTIVKEHVLNEKYDLRYAGS"
     gene            6008..6274
                     /locus_tag="VFC43_06880"
     CDS             6008..6274
                     /locus_tag="VFC43_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30940.1"
                     /translation="MAEEDYRILPAQQGKVQCLPDKKEPVENCGFCIHCREFSVGGKF
                     VKSPSLAYCTKCRVTERVDYTKATAVKCADRQGEGFHSITSLIS"
     gene            complement(6307..7749)
                     /locus_tag="VFC43_06885"
     CDS             complement(6307..7749)
                     /locus_tag="VFC43_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HZK30941.1"
                     /translation="MSLFVMGSASHVGKSVTVAAICRCLIRRGITVAPFKSQNMSLNS
                     YVTPDGGEIGMAQAMQAVAAKVAPSTDMNPVLLKPKGDCISQVVLYGRPYKDVHIAAY
                     YQETPQLLIKALESYERLKSAFGQVVVEGAGGAAELNLYDRDIANTQLAKTLRIPVIL
                     VADIERGGVFAQIYGTIELLPEEIRPLVKGIIINKFRGDPAIFAPGVEKIEELTGIPV
                     LGVVPWFSIPLPREDSLSLGDKKTKQSGVRIGVIRLPKISNFTDFELLEQHAAVEYVP
                     CGGSLDGYDCIIIPGTKNTIDDLHALIRTGTDRRILAAREKGIPVIGICGGYQMLGKT
                     LIDAGFESDAGTYKGLGLLDCVTRFASYEKNTVQVTRKACLVPPLLSAMGEVSGYEIH
                     MGVTESERNREALEGDGRVSDDGLVFGTYLHGLFLNPAAANALLSFLFAKKGLPYTPL
                     PAGTEDPYDALADLYEEYVDMDAIVALLKS"
     gene            7939..8244
                     /gene="yciH"
                     /locus_tag="VFC43_06890"
     CDS             7939..8244
                     /gene="yciH"
                     /locus_tag="VFC43_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HZK30942.1"
                     /translation="MIGGICPTCGLPKELCICEEVAKEQQRINVKVNKRRYGKEVTVI
                     EGLDPTDIDLEDLSKYMKSKLACGGTVKGNSIELQGNHRDRVKELLSLKGYSTENIS"
     gene            complement(8328..9362)
                     /locus_tag="VFC43_06895"
     CDS             complement(8328..9362)
                     /locus_tag="VFC43_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZK30943.1"
                     /translation="MSLKDHVKNMVNRGDKTPPPLKSAELSSVESEVDEITRNLEKER
                     QTREGLGKFLKQPFQVLTEKPANILILTIPLALIIFIGGFVSTVMSYGIGILFTSTLV
                     DDYTVFAVLVAIVPVAVLDFKEQMRIKNLENALPNFFRDLAGMNDSGMTLPNAVHIVA
                     GAEYSTLTPHIRKLDQDMSWGAGFVEAMFRFGRDLGTPLADRSVDLIAKASKAGGDIS
                     EVLRAAAKDTFEVVNLSQERSNNMLIYVIIVLVSFAVFLFVIAILVSTFLTTMATAGA
                     SAAASGARGFMGQIDLFQYKRLFTHAGMLQGFFSGLVAGQMGEGRLIAGLKYSAIMLF
                     IAWVTFRFFI"
     gene            complement(9364..10287)
                     /locus_tag="VFC43_06900"
     CDS             complement(9364..10287)
                     /locus_tag="VFC43_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZK30944.1"
                     /translation="MNSFERFCFNLLGKRMKDKREDYAALRNDLISARFKTPFEVYLS
                     TAYISAIIVGLAGAVVIGFVSWIFQLPNLIKYRGEVPEILVGLSAHTLLLGTIFITIF
                     SLLVFGGLTYLAFILYPGLEAGNRRRNIDASLPYAINYITSMSTAGITPAEVFRLLGS
                     STIYGQSAVEARYISREIDIFGRDLIDALRLVSSSTPSSRMKEFLQGTMASVASGGNI
                     TDYFRTKAEQYALENRQTQKMFLETLALVSESYVTAMVAGTLFLIILQSIMSVLSGDN
                     KPMFLYAVIYVMIPLGSVMFVIMISSMTPES"
     gene            complement(10280..12488)
                     /locus_tag="VFC43_06905"
                     /pseudo
     CDS             complement(10280..12488)
                     /locus_tag="VFC43_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285083.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
BASE COUNT         3211 a         3110 c         3137 g         3178 t
ORIGIN      
        1 cacctcacgg gtcataccag ctgcaggtaa aagagatgag aaaagccggc ctcggggaga
       61 aattcctgct tgtcgagcag tggaaaaagc agcttgccgc tgaaggatgt ttttctttag
      121 aacggaaacg ctgcctcccg cctttccccg cacgggtcgg cgtggttact tcagaaacgg
      181 gtgcggtaat ccatgatatc aggaccgttg tcggtcgacg ctaccccctc gagattatta
      241 tatcccctac agcggttcag ggcgagcttg cacaccggga gattgccgct gcgatttgcc
      301 gtatatcggg tcttgtggac gtggtgatcg ttgcccgcgg gggaggcagt tttgaggatt
      361 tgttcccctt taaccacccg gatgttgtgc gggcaattgt tgcttgtcct gttccggtcg
      421 tcagtgcaat cggtcatgaa gtcgatgtga ccttaagcga tctcgccgca gacctccggg
      481 ccgctacacc ctccgcggca gcagaactgg tggtaccgga ccggctggtg ctgacagccc
      541 ggctgcagga actcagaagc cagctgaacg ctgccctgca ggcccgggtg gcatgggcat
      601 gggatgagat ttctgacctt cgggacaggc tccgcccgca gaggtttaca aggaagatcg
      661 aagagagaaa acaaaacacg gctgacctgt cggaacggct cgaacgggct ttttcaaacc
      721 ggctcgggcg tgagcgcctg ctcctggcag agatgagaac tgcactggac agcaggaacc
      781 cggttgcagt ccttgcccgg ggctattgtg ttgcagagaa gcagggcagg gtcgtgaaaa
      841 gcgcagggac cataataaag ggagaccgta tgaacatcag gttttatgat ggcagcagcc
      901 aggtaaccgt ggaggacgtg aatcatgaaa gagacctatg aggtaaagat agaacaatta
      961 cgaaagatca ttgagaagat cgaggacggt aataccggtc ttgatgaaag catgaaactg
     1021 tatgaacagg gtgcggtgct ggtaaaacaa tgcgaagtac tgctcactga agcggaatta
     1081 aaaattacca cgctcagccg ggacgcgtag cggtcagggt cacggtcatc tcttttccca
     1141 aaacaaggtt tgcgataagt gcttccggca ggtccgccgc cacatgatcc gagaggatac
     1201 cgattgtccg cccgcagaca aaggtactcc tgcgccacac catatccgta ggatggtcaa
     1261 gagtaaatgc cgatgagccc cgggatttca cctcggcagt tatccccccg cacgacaggt
     1321 gggtaaagag cactgcgtca tcatggcaaa ggacccgtct gaatcccgca gagagtcctt
     1381 tgcagccctt gtccgcagag ataccgataa tacagttacc atttttcgaa aggtgcccct
     1441 cagcggttac ctcaaatgtc gtcgggtggt tgccaaggac gagtggatgt cccctgcaat
     1501 ggattgtctc ctgagcctcc atgctttatc atgtgagggt tgccattgat attcaatgtt
     1561 tatcaccgtc ccctgccccg agtgcaacga ggagaccgaa cacgagatca taaccgaatc
     1621 ccatgatctg cttgtccgct gcacgacctg cggtcaccac cagcgcatca aaagagagaa
     1681 agaaccccag tcaatcctca ttaaaacgat cgtctccaaa gaagggacat cgttggtctg
     1741 cggcatcgag ctggcagaag atgatgaatg cagcgttggt gaccacctgg ttgcagaatg
     1801 cggggatgaa gcattcgggg ttgaagtaag ctccattgaa tgcggggaga agcgacaaaa
     1861 ccgggcaaaa gctcctgata tcaccacgct ctggacccgc actattgaac aggtagtggt
     1921 aaaaatctcc atccatgacg gcagaaaaac tgccccggtc tatatggaag tagacgggga
     1981 acaaccgttt gtcgtcggag agatgtatgt tgcttcagga aagagattca aagtctctca
     2041 ccttaaactg cgtgacggcc cgctgatgag aaaagaaggc tggaaatcgg ttgcccatcg
     2101 gattaaacgg atctacggta cccgcttata aatattttat aattgccgcc ctgagttgtt
     2161 cgggatgtac tacaccttcg aaccgctcga gaactttctc ctcttttaaa atgattattg
     2221 tcggtgtcac cttcagggta aattctttta taaaatgcgg attttttatt gcatcgatct
     2281 cctcaatcgt tatgttgagc gcttttgcca cttctgcatt tatcggggtc tgctccatgc
     2341 agcccatgca ccccggctgg taaaaacaga tcacacgcac tgccatgaac aatgagtgga
     2401 caagctacgt aaaaaagagt ttgtatggaa gttactgctt gttgatggaa aggacccgga
     2461 tatcgatcga aggataactg aaatcaacga caatcccttc aggggacttg cttgccactt
     2521 caccgatgcg caggaaagta gttgccgtgg tattattcac gaccgcttcg gggttgtccg
     2581 aaacacccat aaagaaatta tcgccgacat tgatatcagt caggttgatc ttgctctgcg
     2641 caagttgttc aacagaccag tcctgagtca ttgaaatgtt cgaaccgatc ggaatccgtt
     2701 tttgttcgtt tactttcatc ccgataattg cagaggttat cacgttatat tccgaggaaa
     2761 aaatcgcgag ctgcttgaca ctggccccgt ttgatgggta taccggaact gaatatatgg
     2821 gtttttcaat cgtctggttg gaaattatcg tcagctgttt tgatccgaaa atctgccggt
     2881 tttttcccac aaccgtgtca taggttgctt tttcagacgt tacgatgggt tgacctgcag
     2941 cgtcaaagat cgtataatca acaacaaccg aatcaccggg tcttactgaa gtaaacatag
     3001 taagccagcc ggaacccata ccggaaacta ccatgagcac gacaaaaagg acacaggcac
     3061 ccacaataag tacctttttc acggttttca tcgtttcttc tttctttttc ttttctccca
     3121 tgtaatcctc attattacat cattgtccaa ttgtcataaa ctattccgaa ggtacaggta
     3181 cgatagggtt ctggtgcgtt tacgggactt ttccgccata aacagggttt tttgcagtac
     3241 gggaacaaat taaaatctcc ccagaatcgc acagagaaac ttaaatactg cttcaatctc
     3301 ctttttccga tgacttttag tactcttttc acttaaatct gtagtgatgg ataaaaaaaa
     3361 ggtcaaggac tatatgacct atgatgtcgt cagtattgat tttcacggca cggtaaaaga
     3421 cattatcgaa aaaattcaaa agacccatca cgacgggttc ccggttgtcg aaggagatga
     3481 ggtggtagga tatgtgtcag cacgggatct tctttttgtc cagctgacca caccggttaa
     3541 agaagtggta tccaaaagcc tcattgttgc cgaccccgag atggatatca atgatgctgc
     3601 acgggtgatc ttccggagcg ggatccagaa acttcctgtc gtggatgaga aaaaccacct
     3661 gctgggcatc atctcaaatt ccgatgttat ccgctcccag atcgagcatg tttcccccga
     3721 aaaagttttc aattttatta ccactttaaa gaagctctat ggcgtagatc ccacgctcga
     3781 ccgtggcacc gtgccggtcc gggatctcct gcctacccag tcaaagatct acgaggatga
     3841 actcgagggc cggatatacg agatcaaaaa aggacttgca gagccgatta ttgtagtaaa
     3901 gcgcccggga aggattattc ttgtggacgg gcatcaccgg gcgatcgctg caaaacgtct
     3961 tggcataacc gcaattgatg cctatattat cgagataaaa gaagatattg aactgggtat
     4021 ggaacgcacc gcacatgcaa tgaacttaaa atcgttggac gatgtccagg tgctcgacta
     4081 tgcccggcac ccccttgtcg cgataaccca tcggctggtc aaaagagtat aaccatctcc
     4141 tgctttttta cccggaaata ttttccgtcg taaaaactca atattttcaa tgaaaaagga
     4201 ttttctctct ctgtagaaac aggcatgaac ctatatgttc tcaccagatc catgaaaatc
     4261 ggttttctct gcgatggaca ccgactcatg gcctctccga ggcacggcgg gacgcggaga
     4321 ttaattgtat ttcagttact gatccgccac gggggcgtcc ctccgggggc agcggcgttc
     4381 atcgtaactg aaaaacagac atgataatcc cttatcaaac ttgattgatt cgattttcat
     4441 gggaaaacct tttttctggt aaaatagtgt ccctcttttt gtgaaaaacc aagtttggta
     4501 gcaggtaata catcgtcatc gcgatgggta tgccccagtg gcgggggtaa agcccccagg
     4561 agtgtcagaa tttttaaatg atttctacaa tcagattttt aagctctatg aacctgcata
     4621 acgaagatcg tacttttcgt tcaggacatg ctctttcact atagtaccat cggataccac
     4681 aacctccggc cagagacggg ttttcgagtg gacaactacc cggtcccgca ggacaacccg
     4741 gggaccgatg accgtgtcgt tctcgatgct gcagtgttcg ccgatatgcg tgtcattatc
     4801 catgatgcat cccgatatgg tagtgtttgc cccgacgatg acacggttat aaagggacga
     4861 tgaaaatacc tttgtgttat ttctcaggat acatttttcc cctatgctcg tgtagggacc
     4921 gattaacaca ttatcaccta tagttgttcc cgggccgata gcaaccggac caataaccct
     4981 ggtgttctta cccagcgtaa tcgaatctcc cagctggacc ggcccttgta tctgtgcccc
     5041 atgcatcgaa agattcccga tgatatcggt aaaagcgata tcctgcagct tccagcgctc
     5101 agcctgccgg agcgagtgcg gacttcccac atcggtccag ttaccccggg caagccagcc
     5161 tttcaggaca aagttttcct ccatcatctg aggaaacagg tcgcgggcga aatcatattt
     5221 ggttgcgggc ggtatatggt caaaaactgc cgggttgcac acatacatcc cggtgcttgc
     5281 gagattggag aagatctcgc cgggtgccgg tttttcctta aaacgtttta tctcgtacgt
     5341 gacatcaatt tcggcaatac catactcgcc ggggtcatca atggagatga gaccgatagt
     5401 cgtggttgcc ttctctttct ggtgggcacg gtaaaactcg agcacattca gatcggtgac
     5461 atggtccccg ccaacaacta aaaagtcctg cccatcgaga taccgttggg cgttttttac
     5521 actgccggca gttccgagct tggttttttc atggacatac gtgatttcca cgccgaagag
     5581 ggacccgtcc cccagcgctt cttcaatcga gctgcccata taccctaagg ttaccacgac
     5641 ctcacggaaa cccatattcg aaagatgtga gaccaagtgc tggatcgagg ggcggttgac
     5701 aatggggata cacggtttcg gcctttcaaa cgtgagcggg cggagccggg tcccctcccc
     5761 accgcacatt atacacacct tcataccaca aattttgtgc tgaagaggtt taaggatagt
     5821 gctggacgaa atttttcctg atcaccggga aaggttaaaa tatgggattt tttggctgag
     5881 atatgcagtt gccggagtta tacgtctcat atagtggcga acatccacat catctgtggg
     5941 aagtcaggtg cccgttcatg ggtattatat ccgacacacg acaaacacat agagcagatg
     6001 agtatccatg gctgaagaag actacaggat cctgccggca caacagggaa aagtacagtg
     6061 tcttccggac aaaaaagaac ccgtggaaaa ctgcgggttc tgcatccatt gccgggagtt
     6121 cagtgtcgga ggaaaattcg taaaatcacc atcgcttgcc tactgtacaa aatgccgggt
     6181 aacagagcga gtggattata ccaaagcaac tgccgtgaaa tgcgctgacc gccaggggga
     6241 ggggtttcac agcataacaa gtctcatcag ttgaacgctg attgatggag aattaaaaag
     6301 accgtttcat gatttcagca gggctactat tgcatccata tccacatatt cttcatacag
     6361 gtctgccaat gcgtcatagg gatcctccgt tcctgccggt agtggtgtat agggcagacc
     6421 cttttttgca aacaggaacg agagaagagc atttgctgct gccgggttta aaaacaggcc
     6481 atgcaggtag gtaccaaaga ccaggccatc atcgctcacc cgcccgtcac cttcaagggc
     6541 ttcacggttc ctttccgatt cggttacccc catgtgaatc tcgtaaccgc ttacctctcc
     6601 catggctgaa aggagagggg ggaccagaca agctttcctg gtcacctgga cagtattttt
     6661 ttcataggag gcaaatctcg tgacgcaatc cagcagtccc aagcctttgt aggtacctgc
     6721 atcggattca aacccggcat ctatgagggt ttttcccagc atctgatatc ccccgcatat
     6781 cccgataacc ggtatgcctt tttcccgggc tgcaagaatg cgacgatccg tgcccgtcct
     6841 gatgagggca tggagatcat cgatcgtatt ttttgtcccc ggaatgatga tgcagtcata
     6901 tccatccagt gatcctccgc aaggaacata ttcaacggct gcatgctgct cgagcagctc
     6961 aaaatccgta aaattggata ttttcgggag cctgataaca ccaatcctga ccccggactg
     7021 ttttgttttt ttgtctccca gggaaagtga atcctctctc ggcaatggga tcgaaaacca
     7081 cgggaccact ccaaggaccg gtatacccgt gagttcctca attttttcca cgccgggggc
     7141 aaaaattgcg ggatctcccc ggaacttgtt gatgataatg ccttttacca ggggcctgat
     7201 ttcttcaggc aggagctcga ttgtgccata tatttgtgca aatacgcctc cccgctcaat
     7261 atcagccacg agaataaccg ggatgcgaag cgtttttgct agctgggtat ttgcaatgtc
     7321 ccggtcatag aggttcagtt ccgctgcacc tccggcccct tccacaacaa cctgtccgaa
     7381 cgcagacttc agccgttcat aggattcgag cgctttaatg agaagttgtg gggtttcctg
     7441 gtaataggcc gcgatgtgaa catccttgta cggacgcccg taaagcacaa cctgcgagat
     7501 acaatcgcct tttggcttca ggaggacagg gttcatgtca gtactgggcg caacttttgc
     7561 agcaacggcc tgcattgcct gtgccatgcc aatctctcct ccatccggtg ttacatagga
     7621 gttcaggctc atattctggg atttgaaggg cgcgaccgtt atcccgcgcc ggatgagaca
     7681 gcgacaaata gccgcaacgg taacactttt tcctacatgg gacgctgaac ccatcacgaa
     7741 cagggacata ccagtactaa tctgtggaat ttcagatcat aaaaaacaag gttcttaaag
     7801 atatattcaa taaaatctca catttactgc gggatccggt cgggtctccc caaaattacc
     7861 cttaatttgg ccaaatacgt cttatttcct tcaggcaatg ccaagtaata aataaccaga
     7921 gacgtaaaaa tacatgtaat gattggtggg atttgcccga cgtgtggact accgaaagaa
     7981 ctatgtatct gtgaagaggt agcaaaggaa caacagagaa tcaatgtcaa agtgaacaaa
     8041 cgccgttacg gaaaagaagt gaccgttatc gaaggactgg atccgacgga catagacctt
     8101 gaagatctct caaaatatat gaaaagcaag ctcgcatgcg gtggaaccgt aaaaggaaat
     8161 tccattgaac tgcagggcaa tcaccgcgac agggtcaagg agctgctttc cctgaagggt
     8221 tacagcacgg aaaatatttc ctgatctttt tttttctttt atcagaacgt tcaattattg
     8281 gtaccaggaa cactgacgtt ctgaattaaa ttttttttaa aaaaagatca gatgaagaac
     8341 ctgaaggtaa cccaggcgat aaacagcatt atcgctgagt atttcaggcc tgctataagc
     8401 cgtccctcac ccatctggcc tgctacaagt cccgaaaaga aaccctgcag cattccggca
     8461 tgggtgaata aacgcttgta ctggaacaaa tcgatctgcc ccatgaaacc cctcgcacct
     8521 gaagctgctg ccgaggcgcc ggctgtcgcc atcgtggtca ggaatgttga aaccaggatg
     8581 gcaatgacga acaggaatac ggcaaaagac acaagcacga tgatcacgta gatcagcatg
     8641 ttgttactcc gctcctggga aaggttcacc acttcaaacg tatcttttgc agctgccctc
     8701 agcacttcgc tgatatcacc accggctttg cttgcctttg caatcaggtc aacactccgg
     8761 tcagcaagcg gggtcccaag gtccctgccg aagcggaaca ttgcttcaac aaaaccggca
     8821 ccccacgaca tatcctggtc aagtttcctg atgtgaggcg tgagagtcga atattcagca
     8881 ccggcaacaa tatgtacggc attgggaaga gtcataccgg aatcattcat gccggcaagg
     8941 tcacggaaga aattcgggag agcattttcc aggtttttta tccgcatctg ttctttaaag
     9001 tccaggactg ccaccggaac gatcgcaacc aggactgcaa agacagtata atcgtcaacg
     9061 agagtcgagg taaacaggat tccgatccca tacgacatta ccgttgaaac aaaaccgccg
     9121 ataaaaataa tcagggcaag cgggatcgtg aggatgagga tgtttgccgg tttttcggtg
     9181 agcacctgaa acggctgttt taagaatttc cccagccctt cccttgtctg ccgttccttc
     9241 tcaaggttcc gggtgatctc gtcaacttcc gattctacgg aacttaattc ggcggatttg
     9301 agaggcggcg gggttttatc gccccggtta accatatttt taacgtgatc tttgagactc
     9361 atttcaggac tccggggtca tggaactgat catgataaca aacattacac tgcccagggg
     9421 gatcatgaca tagatgactg cataaaggaa catcggcttg ttatcaccgg aaagcaccga
     9481 catgatagac tggaggatga tcagaaacag tgttcctgcg accatggcgg tcacatatga
     9541 ctcggacacc agtgccaggg tctctaagaa catcttctgg gtctgcctgt tttccagtgc
     9601 atactgctcg gcttttgtcc tgaaataatc cgtgatgttc cctccgcttg ctacacttgc
     9661 catggtgccc tgtaaaaatt ctttcatgcg ggagcttgga gtactggatg aaacaaggcg
     9721 gagcgcgtcg attaaatccc tgccgaaaat atcgatctca cgggagatat accgtgcctc
     9781 cacggcactc tgcccgtaga tcgtactgct gccgagcaga cggaacacct ctgccggggt
     9841 aataccggcc gtggacattg aggtgatgta attgattgca taggggaggc ttgcatcaat
     9901 gttcctgcgc ctgttccctg cttccaggcc gggatacagg ataaatgcaa ggtaggtcag
     9961 cccgccaaac accagaagag agaagatcgt gataaatatc gttccgagta aaagcgtatg
    10021 tgctgataag ccgaccagta tttcagggac ttctccccgg tatttgatca ggttggggag
    10081 ctggaaaatc cagctgacaa aaccgataac tacggcacct gcaagcccga caataatcgc
    10141 ggagatgtat gctgttgaga gatagacttc aaacggtgtc ttgaacctgg cagaaataag
    10201 gtcatttctc aaggctgcgt agtcctcccg cttatccttc atccgctttc ccagaagatt
    10261 gaaacagaag cgttcaaaac tattcataca ggtccttcct taccttctca ataactgctt
    10321 cgggatcgcg atggtacgaa atgaggatct ttgccacatc cttgtaatgg cggatcttct
    10381 tgatccgcat ccattcaagc acttcctgtc gacgcttcaa ctcctcccgc atgcggcttt
    10441 ctgaccaccc ttttgcctcc atgatctcct cgagaaggta ggacttgccc gaatagatga
    10501 tctcatctgt tgctgaccgc cacttaaaga cctcattggt gatcagttca ttggtcctcg
    10561 ggtcaatgtc caggatctca acgatctgct tgttacgccg gatacgtttg ccaccaacac
    10621 gcccctgcac ctggacacag ataaaatcaa gcgctgaaag catgttacgg ggaacatcca
    10681 tcggggggtt ctcgatacgg tggacgacac tcgcgacgga atctgcgtgg gttgtggagt
    10741 acgttgtatg gccggtgctc atcgcctgga agagtgtctg gcattccttg cctcgcactt
    10801 cacctacaag gatgtattcc ggacgctgac gcatcgcagc ccgcaggagt tcatacatat
    10861 taatctcacc cctgccggaa gtgtcaaacg agtcgcgggt aatgcttgga atccagttcg
    10921 ggtgagggag tttcagtttc acgggtatcc tcaagagaaa cgatctttga catcggcggg
    10981 ataaacagtg agatggcgtt cagggcagtt gtttttccgg atgcagtccc accggcaaat
    11041 attgcagatt ttccgttttc gacacagagc cagatatacg cgatcgaaag cggggcaaag
    11101 gtacgccatt caataagatc ggtcggggtg atcggttcgt ccttgaattt acggatggtg
    11161 aaggtggagc cgtgtgcggt tacctcctgg ccaagcgtca tctggatacg agaaccgtcc
    11221 tgcatcgtcg agtccaggat gggttgtgca attgagatgt attttcctga acgctgggcg
    11281 agtttggtaa caaacgagtc taagtcttct gcactcgaat agctcagggt tgtcttcatt
    11341 gactcgtagt tggagtgata gacaaagagc gaggaattca ccccgtcaca ggagatatct
    11401 tcgatatact tgtcatgcat gattgcatcg atcagaccgt cccccaggaa ttccttgtgc
    11461 atattataga gaatggtctc gcgttgtacc ggattgagtt tgataccaaa gtcctggatg
    11521 acatctgttg ccgcagctct cagtttgatt ccggcttctt ctttcgagat gtcttttgtg
    11581 ttgatatcga gtgtttcaaa caggcgttcc tttaattctt ttaacaggtc tgcttccggc
    11641 tcggtcagaa tgggttcgag cactttgtac gtgtattcgt gggtggaatg atcatagata
    11701 atctgtatat atgcaaacgg gggattaacc ggatagattt ccacctcctc aacgcccggt
    11761 tgaggtttga aactgatatc aacaagcgga ccgtgtttgt tgggatcata ttcttcgagc
    11821 gtggaatgaa ctgccttgaa aagccgggac aacccgaatt ttttctgttc aactttgggt
    11881 ttcgatgctg ctgcggtttc ggcaatatca aggtccttga aacctgcagt cgagaagttt
    11941 ttcttccaga tctcatcgaa atcaggtggc agggtccccg aaccttcaaa tgcgctgatt
    12001 cgtccgtgca gtttgagttc ttcaacttta aacgatacac ctttggggag gataagaccg
    12061 gagatctcgg agatctggtc aacattgaca atcttctgat ctgcgtttgt gacctgtcca
    12121 gcagcagggg gggccaaaac cgcatttttt atcgctttat tccgaacagc tgccttggga
    12181 tcttcatcaa ttatctcaat aacctcttca tgagctgttt cgttgccggg aggcggagcg
    12241 gcggtttgat ttgtccccat accacccaga agtttaccaa gggactgccg tacatccttt
    12301 ccctccatga gagcatcagg ttcggaaggg gccttttgtg acatctgggg agattggggg
    12361 aatttttcct gcagatcatc tgcatcggaa aattccagat catcatgcgg ggatgctggc
    12421 ggagtgcggg gcgtgactga cggctcccgc agcgggggag cgggctcctc tggttttgct
    12481 gtttccattt tttttctgag cgcggcgaaa tcaccaatgc cggatgctga gctgccaccc
    12541 gccgttgatg ctttaagagg agaatcagta tttttctgaa cctgtggctc ctcacttttt
    12601 ttgcctttaa tcctgttgat caaagaatct acatta
//
LOCUS       DASRLV010000176         5269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_157408,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000176 DASRLV010000000
VERSION     DASRLV010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157408"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            421..1146
                     /locus_tag="VFC43_06910"
     CDS             421..1146
                     /locus_tag="VFC43_06910"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HZK30945.1"
                     /translation="MRIIRAPSIGRAHELVIKMILEKGWILHTEDAEATIEFEEVTLQ
                     VDTPLAEPAVSLHSQFQQRFVEQYARDLLHGSHASFEYDYHGRLFDWGERLVAYGQPV
                     HIDQIAYIIEKLKSSPVTRRAIAITWNPVIDQKLDDCPCLQIIQCVVRDGKLNMRVVF
                     RSNDMLTAAGANMYALVQLQKSIADALGMPCGTYTHISLVPHIYFIRDIHDIEPFCGK
                     GKLIQPIKDVCHACQKCERAKEI"
     gene            1202..1274
                     /locus_tag="VFC43_06915"
     tRNA            1202..1274
                     /locus_tag="VFC43_06915"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1236..1238,aa:Gln,seq:ctg)
     gene            complement(1340..2056)
                     /locus_tag="VFC43_06920"
     CDS             complement(1340..2056)
                     /locus_tag="VFC43_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HZK30946.1"
                     /translation="MSNGIRFFSAGPRSVFIIIILVGLVIILIPLLILGMIGAAFTRL
                     GFSWISALIIVLLMLFGSFVNIPVYRIRRDTIRVSQDNSPDFGVCVPFVSDQVWDTML
                     SLNFGGAVIPVCISLYMVYQAIQVTGMPLVLTICSDMIIVVVITFLVTRFVNGVGIQV
                     PLFIPGLTALLAGLLLAGEAGLVAAVTAFVSGTSGVLIGGNILHLFRIKDLEISDVSI
                     GGAGTFGAIFICCILPALIS"
     gene            complement(2066..2674)
                     /locus_tag="VFC43_06925"
     CDS             complement(2066..2674)
                     /locus_tag="VFC43_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrillarin-like rRNA/tRNA
                     2'-O-methyltransferase"
                     /protein_id="HZK30947.1"
                     /translation="MIWVGDVLVSHGEGGVYNERMLGDSRVWDPFRSKLSALYYVGKG
                     IELTHGMRVLYLGAANGTTVSHVADYVDVVYAVEFAPRPMQDLLEVSRRRKNVIPIMA
                     DAARPEQYAPLIEAVDLLYQDVAQPEQASIALRNCSFLKSGGILVLMLKTRSVDVRKE
                     PDEVFKDTLAALTSARLIVQESVWLAPYHHDHAAIICTKPIV"
     gene            complement(2671..3534)
                     /locus_tag="VFC43_06930"
     CDS             complement(2671..3534)
                     /locus_tag="VFC43_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005730 - nucleolus [Evidence IEA];
                     GO_function: GO:0030515 - snoRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NOP5/NOP56 family protein"
                     /protein_id="HZK30948.1"
                     /translation="MQSYWFGDVGDGHCTLFIGDSNAVIDRIRSVSTSMDSFIPLDWE
                     QAVLCGACRDRADYLARLQALCIADAERSIREQYSRKDAELLQMVRTLDEIDNVINLL
                     SERTAEWYLVRHPAFSRKYRRTPAHVLVKTMREKSKGALGSVAAEIEQLAETRATLAK
                     EVSSRANSVMPNTSALIGGLVAARLLSHAGGLLLLSRLPASAIQVLGARTALFAHLKT
                     QSPCPKHGIIFQHRRVHNAPRNVRGKVARVLAGKLAIAARLDYFRGFIAPEFLDAAQK
                     RIDTTGRGSEP"
     gene            complement(3611..4825)
                     /locus_tag="VFC43_06935"
     CDS             complement(3611..4825)
                     /locus_tag="VFC43_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HZK30949.1"
                     /translation="MIEILLGTLIGVILGTVSGIIPGVHANTLAGVLLSLQVVLLSFF
                     GPLVLAGAMFAALITHTFVDAIPSTFLGIPDADTSLAVLPAHALCLEGNGEEAVRIAA
                     LGSACAMVIAVPVSVICFFVLPALQPYFDWWIGILLIATIGYMIVSGESPGWALLLFC
                     ASGILGIFSLRYAFLSWHTLAGSSAILMPLLTGLFGISVLITASQGTIPEQHYRGIRM
                     EDRTIMKYSALGTVAGVAVGWLPGLSTASANSVLASIIGYEKNRRTYILATSAANTSN
                     AFIGLAALFALSRMRNGVMVALSELPLPTMSELTVVGVLAACAAYGITVVLSRSASGL
                     NGIDGKLLNHAVIAFIVILCIILTGPFGVVILILATALGLVPYLVNIPRVFCMGAIIV
                     PVILYSFNIAWI"
     gene            4904..>5269
                     /locus_tag="VFC43_06940"
     CDS             4904..>5269
                     /locus_tag="VFC43_06940"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HZK30950.1"
                     /translation="MKIAVTRLAGKEKNDAARCAQFGHICYSVHPLRSEIRPHTITAF
                     IEAVDRNEFDCLFFTSALPAKIIAPLLNRFPRIIAIGPQTAKELQTYEIDCETLTSFY
                     SKDFVPYLGDWIRGKHIGIP"
BASE COUNT         1502 a         1299 c         1234 g         1234 t
ORIGIN      
        1 atactccgtt tagacggaat atggaagagt atttgttgaa gacctaatat atcctttgtg
       61 gtcggttttt tagaggaatc tgggcaatat atcgagcctg tttcttattt cgagcgaatc
      121 aattaggttt tgtactcaaa attgaacaac ctcagttgcc caacatgaca ctggtattat
      181 tgtttgtgca agtataatga taatcataaa tatcgtataa tttaaatgag atatgataaa
      241 atatattatt gattttctat accttaacta atagtataaa attttttatg gtgtcttccc
      301 ccggtgtcaa ttcctattag taaatttcgt gtttttacga tttacactaa ttttttttaa
      361 actcattatc ccacaaatca tttcaacaca tatacaatgt acgatgacaa acatttttcc
      421 atgagaatta tccgggcgcc gagtatcggg cgggcacatg aattggtcat caaaatgatc
      481 ctggagaaag gatggattct tcacactgaa gatgctgagg caactatcga attcgaagaa
      541 gttacactgc aggttgatac ccccctcgca gaaccggcgg tgagcctgca ctcacagttc
      601 cagcagagat ttgttgaaca gtatgcccgc gatctgttgc acggttcaca tgcatctttc
      661 gagtatgatt atcatggccg gctctttgac tggggtgagc gtctggttgc ttatgggcag
      721 ccggtgcata tagatcagat tgcctacatt attgaaaaac tcaaatcatc accagtaaca
      781 agaagggcaa tcgcaataac ctggaacccg gtgatcgatc agaagctcga tgactgcccc
      841 tgcctccaga ttatacagtg tgtcgtccgt gacggaaaac tcaacatgcg ggtggttttc
      901 cggtcaaatg atatgcttac tgctgccggt gccaatatgt atgccctcgt tcagctccag
      961 aaatcgatag cagacgctct tggaatgccg tgcggcacgt atacccatat ttcacttgtc
     1021 ccccacattt attttatacg agatatacac gatatcgaac cattttgcgg aaagggaaag
     1081 ttaattcagc cgatcaagga tgtttgtcat gcctgccaga agtgcgaacg ggcaaaggaa
     1141 atatgacaat tgtcagtccg tcaccttaat taagcaacag ggcaaaaaat aagaggcaca
     1201 aagcccggta gtgtagcggt caatcatggg ggactctgga tcccccgaca gcagttcgaa
     1261 tctgctccgg gctactactt tcttggtgtt ttttaataaa aaatcagaac tcatctgaca
     1321 ttcctgcgta cgttcttaat tatgaaatca aagcaggaag gatgcagcaa atgaatatgg
     1381 ctccaaacgt acctgcacca ccaatactaa catctgatat ttcaagatct tttatgcgga
     1441 aaagatgcaa gatgtttccc ccaatcaata ctcctgaagt tccgctgaca aatgcggtta
     1501 ctgctgctac aagcccagcc tccccagcaa gaagcaaacc tgccagaagt gcagtgagtc
     1561 ccggaatgaa aagtggtacc tgtataccga caccgttgac gaaacgcgtt actaaaaaag
     1621 tgatgacgac tactatgatc atatccgaac atattgttaa aaccaatggc attcctgtaa
     1681 cctggattgc ctgatatacc atgtataacg atatgcacac aggaataacg gcccccccaa
     1741 agttaaggga cagcattgtg tcccacacct gatcagatac gaagggaaca catacaccaa
     1801 agtcggggga attatcttgg gaaacccgga tagtgtcgcg ccggattcga tacaccggga
     1861 tatttacaaa actcccaaac agcatcagaa gcacaataat gagtgcagaa atccacgaaa
     1921 accccagccg ggtgaatgct gcaccgatca tgccgagaat gagcagtggg atgagaatga
     1981 tcacaagccc gacaagaatg ataataatga acacggaacg cgggccagca gaaaagaaac
     2041 ggattccatt actcatgggt atttctcaca caatcggttt cgtgcagata attgccgcgt
     2101 gatcgtgatg gtagggggca agccatacac tctcctgcac aataagcctt gcagaagtaa
     2161 gtgcggcaag ggtatccttg aatacttcat caggttcctt tctcacatca acgctccgcg
     2221 tcttgagcat gagaaccaga attcccccgc tttttaagaa tgaacaattc cgcagggcaa
     2281 tacttgcctg ttccggttgc gccacatcct gataaagcag atcaactgct tcgatcagcg
     2341 gggcatactg ttcaggacga gctgcatctg ccataatcgg gatgacattc tttcgtcgcc
     2401 gggatacttc aaggaggtcc tgcataggtc ggggtgcaaa ctccacagcg tacacaacgt
     2461 cgacataatc tgccacatgg gagactgttg tcccattcgc cgctccaaga tacagcactc
     2521 gcattccgtg agtgagctcg atacctttac caacataata gagcgctgac aacttgcttc
     2581 ggaaagggtc ccacacccgg gaatcaccta gcatccgttc gttgtagacg cctccctcac
     2641 catgagatac taacacatca ccaacccaga tcacggttcg ctccccctcc ctgttgtatc
     2701 aatgcgtttt tgtgctgcat ccagaaattc cggtgcgata aatcctctga aatagtcaag
     2761 tcgagccgca atcgcaagtt ttccggcaag tacccgggcg acttttccac ggacattacg
     2821 gggtgcgttg tgaacccgcc ggtgttgaaa gatgatcccg tgcttggggc agggggactg
     2881 tgttttaagg tgggcaaaca gtgccgtccg tgcacccagt acctggatcg cgcttgccgg
     2941 tagccgggag agtagaagaa gccctccggc atgcgacagg agtcgtgcag caacaagccc
     3001 accgatgagt gcacttgtgt tgggcattac actatttgcc cgtgacgaaa cctcttttgc
     3061 aagggtggcc cgggtttccg cgagctgttc gatctccgcc gccacactgc caagagcccc
     3121 tttactcttc tcgcgcatgg ttttaaccag cacatgtgcc ggagtcctgc gatacttgcg
     3181 ggagaatgcc gggtgccgga ccagatacca ttccgcggtt cgttcagata gaaggttaat
     3241 aacgttatca atctcatcaa gtgtccttac catctggagc agttccgcat cttttcttga
     3301 atactgctcc cgtattgaac gttcggcatc ggcaatgcac aacgcttgta atcttgcgag
     3361 ataatcggcc cgatcacgac acgcaccaca aagaactgcc tgctcccagt cgagcggtat
     3421 aaaggagtcc atactggtgg aaaccgatcg gattcgatcg attacggcgt ttgaatctcc
     3481 gataaataat gtgcagtgtc catcaccaac atcaccgaac caatatgact gcatcagtga
     3541 aaatttaggg ttcgtatcgt ataaaaccat agatcgcatg gggcgggatg atgtggaatt
     3601 aataaacaga tcatatccat gcaatgttaa aagagtaaag tatgactggc acgataatcg
     3661 cgcccatgca gaaaaccctc gggatattta caagatacgg cacgagtccc agagccgttg
     3721 caaggataag aataaccacg ccgaacggtc ccgtgaggat aatgcaaagg attacaataa
     3781 acgcaataac cgcatggttc agcaattttc catcaatccc gtttaaaccg ctcgcagagc
     3841 gggacagcac taccgtaata ccgtaagcag cgcaagcagc aagaacacca acgacggtca
     3901 attcgctcat ggtgggtaac gggagttcgg aaagggcaac cattacaccg ttccgcatcc
     3961 gggagagtgc gaacagcgca gcaagtccga taaacgcatt tgatgtgttc gccgcacttg
     4021 tcgcaaggat atatgtccgg cggttcttct catagccgat tatcgaagcc agtacactgt
     4081 ttgcagatgc agtggagagt cccggaagcc acccaaccgc gactccggca actgtaccga
     4141 gtgcggaata tttcatgatt gttctgtctt ccatccggat acctcggtaa tgctgctccg
     4201 gaattgttcc ctgcgacgcg gtgatgagga cagaaatacc aaataacccg gtgagaagtg
     4261 gcattaggat cgcactgctt ccggcaagag tgtgccagct gaggaaggca tagcggagtg
     4321 aaaaaatccc aagaatcccg gatgcacaaa atagtaaaag tgcccagccg gggctctcgc
     4381 cggaaacgat catataccct atcgttgcaa tcaacaggat accaatccac cagtcgaagt
     4441 agggctggag tgcgggaaga acgaaaaaac agattactga taccggtact gcaataacca
     4501 ttgcacaggc actgccaagt gctgcaattc tcacagcttc ctcgccattt ccctcaagac
     4561 acagagcatg ggccgggagt acagcaagtg aagtatcagc atccggaata cccaggaatg
     4621 tgctggggat ggcgtccaca aatgtgtgcg taatgagggc ggcaaacata gcgccggcaa
     4681 gcacgagagg cccaaaaaac gacaggagga ctacctgtaa acttaaaagc accccggcaa
     4741 gggtgtttgc gtggacaccg ggaatgatcc cacttacggt accaagtatt accccgataa
     4801 gcgtaccaag cagtatctcg atcattcagt agagatgtag gagaatggac ataaattcac
     4861 cgaaatgcat accatcatat cattataagg tgatcaatcc ggaatgaaga tcgcagtcac
     4921 ccggcttgcg gggaaggaaa aaaacgacgc cgcacgctgc gcccagttcg gtcatatatg
     4981 ctacagcgtc caccctctac gatcggaaat aagaccacac acaattacgg cattcatcga
     5041 agcggtggac cgcaatgaat tcgattgtct gttttttacc agcgcactcc cggcaaaaat
     5101 catcgcaccg ctcctgaaca ggttcccccg gataattgct atcggaccgc agactgcaaa
     5161 agaacttcag acttatgaga tcgattgtga aactctgacg agtttctatt caaaagattt
     5221 tgtaccatat ctgggagact ggatccgggg gaaacatatt ggcatcccg
//
LOCUS       DASRLV010000177         3458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_552141,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000177 DASRLV010000000
VERSION     DASRLV010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552141"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            531..602
                     /locus_tag="VFC43_06945"
     tRNA            531..602
                     /locus_tag="VFC43_06945"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:563..565,aa:Ala,seq:tgc)
     gene            complement(628..1074)
                     /locus_tag="VFC43_06950"
     CDS             complement(628..1074)
                     /locus_tag="VFC43_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30951.1"
                     /translation="MESLESKLDRLTPDQRKEIEDFVDFLLYRSGNLPVAHGAAQSPV
                     SRQNVASLPLILQEPVHIMENTPLERHDSSPVENSFSPLNNEERATPIQEIIVGGEDP
                     ISRDYMDYGSFEKNPSPATIAVKTVKEKLQKREEQEKPRVSLDWIE"
     gene            complement(1228..2091)
                     /gene="thiL"
                     /locus_tag="VFC43_06955"
     CDS             complement(1228..2091)
                     /gene="thiL"
                     /locus_tag="VFC43_06955"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HZK30952.1"
                     /translation="MDDRELLNVVMGIVGKEKCRDDCAEVPFGHQIMVVTTDMLHRTT
                     DFVDGMSTWQMGWMSAAVTLSDIASMGAEPKYLLIAVGLDAWQDLAGVMQGAQDCCTM
                     FGAEIIGGDIDRHGELTIVTTGLGLVERSSIARRTGAQPGDLVCITGIPGQAQAYLDG
                     YPQYQKALFEPQPRVKEGRLLARGGVSAMMDDSDGVALSLFDLMSVNECGFSLDSARL
                     PFPSGIPADQARELALYGGGDYELIFTLPRHRHPVAGVSYTVIGTATSEKSVLIDSRP
                     VEKRGYQHHWE"
     gene            complement(2084..>3458)
                     /locus_tag="VFC43_06960"
     CDS             complement(2084..>3458)
                     /locus_tag="VFC43_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF460 domain-containing protein"
                     /protein_id="HZK30953.1"
                     /translation="CSRVAFRVFAKRDQVPVSTYHGADVQVRISGKRLERIRFRPLSG
                     KPRYLIVGIDPGTTTAVAALDLDGNLLHLASSRQMNMGDVVESLCRVGKPLIIASDVQ
                     EMPYSVEKIRRAFSAVAYTPKQDVSVETKIELTTSFAYGNDHERDALSAALDAYRQYR
                     HKFQNLLKRIPPGHDLDEVRARVIRGQSLDKVIGDMNSVTPVRTVDAPAAPLEVKHDE
                     KVRVLDGMVKRLRTFVSELQEEVKEKDYEIRRLQARVRKIHTSHDAQVAKDAEVVKKD
                     GIIQGLKKRLRKEERHSRNLSRRLVRIKQFAELSMEGDVLPVKVMEALTKEGLRRLNE
                     DVGIDEGDIIFVTRLDGWGRSVVKELAEIKIRAIVTSAPLLAECDSQMLPVFRELGLP
                     FLSDKDAGVQVRGKRGLCGKDTFETALARWQEEQVQHEREKKSEMIEHMFKEYKSERG
                     KEVRKSG"
BASE COUNT          813 a          944 c          799 g          902 t
ORIGIN      
        1 catccgctcc cgtcggaaat cgctgaataa ttccggcccg gttccgcatt tttctaaaat
       61 tatgacctct aaatcggggg gggtggggga aatgaggtat aaccgggctc gtagcaggtt
      121 ccggtgtacc agaattctca cagggttttt ttgacgataa aaatagcccc agatgacaat
      181 aacacccggc ctcgtcttcg gtgtgattgc tcctttaaaa gaggatttcc catgaaaatc
      241 aaatcattca ggactgatga gggtttatca tctcaattct tcagttacga tgaacgccgc
      301 cgcccccgaa gggacgcccc cgcggcggat taatgactga aaaattccct agccgccgtg
      361 cctcggagag gccctgaggc ggggtccttc gtatagaaaa acgattttca tgatttgggt
      421 gcgaacgctt tcggtttcat gtctgtttct gcagagcgct ttttcaccat tgatcagtaa
      481 caactatatg gaatccgcgg aaaaatagta tgccgcaacc aaaatgcaaa gggctcgtag
      541 atcaggggta gatcgtcacg tttgcaacgt gaaggccacg ggttcaaatc ccgtcgagtc
      601 cactcgtttt ttgttatttt tataaaatca ttcaatccag tcgagcgata cccggggttt
      661 ttcctgctct tcacgctttt gtaacttttc tttcacggtt tttaccgcaa tggttgcggg
      721 tgacgggttt ttctcaaaac tcccatagtc catataatcg cgggagatcg ggtcttcacc
      781 accaacgatt atctcctgga tcggcgttgc ccgctcttca ttattcagag gtgaaaatga
      841 attctcaacc ggggatgaat catgtctctc cagaggagta ttttccatta tgtgaaccgg
      901 ctcctgtaag atcaaaggaa gagatgcgac gttctgccgg gaaacggggc tttgtgcagc
      961 cccatgagcg acaggtaaat ttcctgaccg gtacaagagg aaatcaacga aatcttcaat
     1021 ttcctttctc tgatcaggag tcagccggtc cagtttactc tcgagggatt ccatacggca
     1081 tgccaaaaat tcttgtgttt tagaaatatc tctccttatt ggtatttata gccaaagacc
     1141 tgcatactta tgccttcgtt atagcgatta aaaaactgca aaaaaaggat gcgggaaaaa
     1201 agattgtgtt tcctttatca tgaggactta ttcccagtgg tgctggtagc ctcgtttctc
     1261 tacgggcctg ctatcgatca ggactgattt ttcggaggtc gcagtcccaa tgacagtata
     1321 tgatacacca gcaaccggat ggcggtggcg gggaagtgta aagatcagct cgtaatcgcc
     1381 tcctccgtac agtgccagtt cccgtgcctg gtctgcagga atccctgagg gaaagggcag
     1441 tctggctgaa tcaagggaaa aaccgcattc gttgacactc atcaggtcaa agagcgagag
     1501 tgccaccccg tcgctgtcat ccatcatggc actgacaccc cccctggcta aaagccgccc
     1561 ttcttttacc cgtggttgtg gttcgaagag tgctttctgg tactggggat acccgtcaag
     1621 atatgcctgt gcctggccgg gtatcccggt tatacacaca agatctcccg gttgagcgcc
     1681 ggtgcgacgc gcaatggagg acctctctac aagtcccaga ccggtagtca cgatagtgag
     1741 ttcgccatgc cggtcaatat cgccgccaat aatttcagca ccaaacatgg tgcaacagtc
     1801 ctgtgcgccc tgcatgaccc cggcgaggtc ctgccatgca tcaagcccga ctgcaataag
     1861 gaggtatttt ggttctgcac ccatactcgc aatatcggag agcgtgactg cagcactcat
     1921 ccatcccatc tgccacgtgc tcataccgtc aacaaaatcg gtagtacggt ggagcatatc
     1981 cgttgtcacc accatgatct ggtgaccgaa aggcacctcg gcacagtcat cacggcattt
     2041 ctcttttccg actattccca ttactacgtt caacagttca cggtcatcca ctcttgcgca
     2101 cctccttacc ccgttcgctc ttatactctt tgaacatgtg ctcaatcatt tcagatttct
     2161 tttcccgttc atgctgaacc tgctcttcct gccatcgtgc aagtgcagtc tcgaacgtat
     2221 ccttcccaca aagtccccgt tttcccctta cctgaacacc ggcatctttg tcagaaagga
     2281 agggaaggcc taattcccgg aataccggga gcatttggga atcacattca gcgagcaatg
     2341 gagcagaggt aacaatcgct cttatcttaa tttctgcaag ctctttgaca acacttcttc
     2401 cccacccatc aagcctggtt acaaagataa tatccccctc atcaatcccg acatcctcat
     2461 tcagccggcg aaggccttct tttgtcaggg cttccatcac ttttaccggg agaacatcac
     2521 cttccatgga taattcagca aactgcttga tgcgaacgag ccgccgggaa agattcctgg
     2581 aatggcgctc ttccttccgc agccgtttct ttaaaccctg tataatacca tcctttttta
     2641 ccacttcagc atcctttgcc acctgtgcat cgtgtgatgt gtggatcttc cgcacccggg
     2701 cctggagcct ccgtatttca tagtcttttt ccttgacttc ttcctgaagc tcagaaacaa
     2761 acgtacggag gcgtttcacc atcccatcaa gtacccttac tttctcgtca tgcttcactt
     2821 caagaggagc ggcgggagca tcgacggttc ggaccggcgt tacagaattc atatcgccaa
     2881 tcaccttatc gagcgactgt cccctgatta cccgtgcccg gacttcgtcg agatcatgac
     2941 ccggggggat ccgtttcaat aagttctgaa atttatgccg gtactggcga tatgcatcaa
     3001 gagcggcaga cagtgcatcc cgctcatgat catttccgta tgcaaaggat gtcgtaagct
     3061 cgatcttggt ctcaacactc acgtcctgtt ttggggtgta tgctaccgca gaaaaggccc
     3121 tccgtatctt ttccaccgag tagggcattt cctgcacatc cgatgcgata atcaggggtt
     3181 ttcccacgcg gcagagtgac tctaccacgt ctcccatgtt catctgccgg gaactcgcca
     3241 gatgaagcag gttgccatcg agatcgagcg cagcaacagc tgtagttgtt ccaggatcga
     3301 tgccgacaat caggtaccgc ggcttgccag ataatggacg gaaccggatc ctctcaaggc
     3361 gtttgccact gatccgtacc tgaacatcag caccatggta ggttgatacc gggacctggt
     3421 ctcttttcgc aaagacccgg aacgctaccc tgctgcac
//
LOCUS       DASRLV010000178         2367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_553856,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000178 DASRLV010000000
VERSION     DASRLV010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553856"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VFC43_06965"
     CDS             complement(<1..891)
                     /locus_tag="VFC43_06965"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HZK30954.1"
                     /translation="MGSTEDILKKYWGFTTFLPHQKEIIESVIKGNDTLAIMATGGGK
                     SLCYQLPALYLGGLTLVISPLISLMKDQVDDLNARGIPAAAWNSSSDYKERLEIESGL
                     KNDTIPLLFISPEKFMQPNFLDSLLTSRIRLVAIDEAHCISEWGHDFRPEYRQLAILK
                     KQFPHIPLIALTATAIPQVRKDIREQLGLVNVKEFTGSFNRQNLQYRIIPKKNSKITL
                     LNYIGQHKNDSGIVYCLSKKETEDVAGELRKRGYNGIAYHAGLPKPRREKVQDEFIHD
                     NVKIICATVAFGMGINKPDIR"
     gene            complement(1269..>2367)
                     /locus_tag="VFC43_06970"
     CDS             complement(1269..>2367)
                     /locus_tag="VFC43_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZK30955.1"
                     /translation="GIRRTIKTGYNLRNPLSMNSTPSLSRVSGIMIALAAAIIIFAGI
                     QAASSILGPFILSVYLTLILGTLLHWFERKGLSTCLALILTLIIFFAIIAVFIGMIAG
                     SFIQAMSDLPGYLEKLESSIASASPFLISIGIDPATLTLQNLIQSFSAEIRGLISNLW
                     DLASPLMLIFLTTLFLLFEAKGFSQKLQIIIGEIRPGDLDRFIDLAQKNVDYLVIRTE
                     VNLATGIGTAIILSLLGVKYAIFWGFSAFLLGFIPNIGFWIAIVPPMLLAWFDLGPFH
                     AILVLAGSGLMDTISEYILFPHFTARGLELSTAVVFISLFFWGWILGGIGVLLAVPLT
                     LLVQMACELSDETRWIGFLLGTPPDKNEEKK"
BASE COUNT          642 a          511 c          629 g          585 t
ORIGIN      
        1 ccggatatcg ggtttgttga tgcccatccc gaatgcaacg gttgcacaga taattttcac
       61 attatcgtgg ataaactcgt cctggacttt ctcccgccgt ggttttggga ggccggcatg
      121 gtaggcaatg ccattgtatc cccgtttgcg gagttctcct gcaacgtctt ctgtctcctt
      181 tttactcagg cagtacacga taccggaatc atttttatgc tgaccgatat agttgagcag
      241 tgttatttta gaatttttct tgggtatgat ccggtactgc aggttctgcc ggttgaaact
      301 accggtaaat tcctttacgt tgacaagacc cagctgctcc cggatgtcct ttctcacctg
      361 gggtatggca gtagcagtca gcgcaataag cggaatgtga gggaactgtt tttttaagat
      421 ggccagctgg cggtattcgg gcctgaagtc atggccccat tccgagatac aatgggcttc
      481 atcgatggcg accagacgga tgcgggaggt aaggagagaa tccagaaaat tcggctgcat
      541 gaatttttcc ggagagataa acaggagggg gatcgtatcg tttttcagcc cgctctcaat
      601 ttcgagccgt tccttatagt ctgaagaact gttccaggct gcagcaggaa tcccacgggc
      661 attgaggtca tccacctggt ctttcatcag cgagatcagg ggtgagatta ccagtgtcag
      721 gccaccaagg tatagtgccg gcaactggta acagagcgat ttacccccgc ccgttgccat
      781 gatcgcaagg gtatcgtttc ctttgatgac ggattctatg atctctttct ggtgaggtaa
      841 aaatgtggta aagccccagt attttttcaa aatatcttct gttgaaccca tcgcgacaac
      901 caggaatgta atggtactgg gatgggagac tatttatcgg atatcttaaa ggatcccggg
      961 gaggggggcg aacgtttcac cgattattcc aaggatattt catgtcatta tacctggtaa
     1021 aatttctcgt gctgtggatt ttgtgcacat tgattaaacg ggtaacatta tttcataaac
     1081 gtggcaatac tgccagagga tcaaccttca aaatataagg agtcgtagag agtatatcaa
     1141 caaagcccga agttatatag attataaccg gtattaccag actacttgtt taattttgga
     1201 tgattttgtc cagggatgca gggggtcgtt atccggaaat caccaatggt attgtctttt
     1261 cagattactc actttttttc ttcattctta tcaggcggtg tcccgagcag aaagccgatc
     1321 caccgggtct catcagacag ttcacaggcc atctgaacaa ggagagttaa gggaactgcg
     1381 agaagaacac cgattccgcc aagaatccat ccccagaaaa aaagagagat gaacacaaca
     1441 gcagtagaga gttccagtcc ccgggctgtg aagtggggga acaaaatgta ttcggaaatg
     1501 gtgtccatca gcccggatcc ggcaagtact aggatggcat ggaaaggccc gaggtcgaac
     1561 caggcgagga gcatgggcgg aacaatggca atccagaacc cgatgtttgg aatgaaaccg
     1621 agcaggaaag ctgaaaaacc ccagaatatg gcatatttca ccccaagaag tgacagaatg
     1681 attgccgttc caatacccgt ggcaaggttc acctcagtcc tgatgacgag gtagtcaacg
     1741 tttttttgtg cgagatcaat aaaacggtca aggtcaccgg gccgtatttc tccaatgatg
     1801 atctgcagct tttgcgagaa cccttttgct tcaaagagta aaaagagggt agtgagaaag
     1861 atcagcatca ggggacttgc aaggtcccag agattgctga tcagaccgcg gatttcggca
     1921 gaaaatgact ggatcaggtt ctggagggtc agggttgccg gatcgatccc gatcgagatg
     1981 aggaacgggc tggcggatgc aatgctgctc tcaagtttct ccaggtagcc gggaagatcg
     2041 gacatagcct ggataaagga cccggcgatc ataccaatga aaacagcaat gatagcaaaa
     2101 aagattatca gggtgaggat aagggccaag caggtagaga gaccttttct ttcaaaccag
     2161 tggaggagcg tgcctaagat aagcgtaagg tagaccgaca ggataaacgg accgagaata
     2221 gaggatgcag cctgtatccc ggcgaagatt attattgctg cagccagggc gatcatgata
     2281 ccggacaccc tggataacga tggagtggaa ttcatggaaa gcggattcct gagattgtac
     2341 ccggttttaa ttgtacgccg gataccc
//
LOCUS       DASRLV010000179         3471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_880236,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000179 DASRLV010000000
VERSION     DASRLV010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880236"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1076)
                     /locus_tag="VFC43_06975"
     CDS             complement(24..1076)
                     /locus_tag="VFC43_06975"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HZK30956.1"
                     /translation="MVGIITYGVYIPRYRIKVEEIARVWRANAADISGGLGVFEKSVP
                     DLDEDAATIAVEAARNALLRRTINPEDIGAVYVGSESHPYAVKPTACTVGEAIGATPN
                     MTAADFEFACKAGTAAIQTCMGLVKSGMITYGLAIGSDVSQGAPSDALEYTAAAGGAA
                     FLIGRDDPIATIHHTCSFTSDTPDFWRREGQDYPRHGGRFTGEPGYFKHVEGASRLLF
                     EQTGKSPKDYTYAVFHQPNAKFPQKAAKNLGFTSEQIKPGLVVPWLGNTYSGASPIGL
                     AATLDIAKPGDTIFVCSFGSGAGSDAFDIEVTDTIHQGKFRRDAAPGVKKLLENPIYL
                     DYARYAVHKGKIVMQL"
     gene            complement(1070..1780)
                     /locus_tag="VFC43_06980"
     CDS             complement(1070..1780)
                     /locus_tag="VFC43_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZK30957.1"
                     /translation="MKSGLRNQLAEKMAGEITLSDSPGHALKKWRVNFEIAPGILADR
                     LGVSPSVISDYESGRRKSPGTAIVGKIVDTLLDMDEENDGKHIQKFSTMLFSNIEDDV
                     IYDIHEYATAVPLKDFSEAIGCTLLCGSIDQVLFGYTVVNSLNAIMQFSSDEFNRIYG
                     WSTERAVVFTNVSTGKSPMVAIRVTPFKPRVVVLQGIDIADVHPIVEKMAERDRITVM
                     CTTMEIDKIVSTLREKEW"
     gene            1905..2570
                     /locus_tag="VFC43_06985"
     CDS             1905..2570
                     /locus_tag="VFC43_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF120 domain-containing protein"
                     /protein_id="HZK30958.1"
                     /translation="MIPAEDLECLKVIALRGGCKCPVFVSSQTIGEMLGISQQTASRR
                     LKGLESQGFITRTIAPDGQHVTVTSTGEEELRKEYQAYCRIFSERNIGYVLTGTVVSG
                     IGEGKYYMSLEPYKQQFKTLLGFEPYPGTLNIRLPASRLPVRKKIDGLDWIRIKGFST
                     DGRTFGDAKCLPCRIGDIACGIVVPGSTHYPEDIIEVIAPIALRRKLGVEDSDTVTVE
                     VGQ"
     gene            2567..3244
                     /gene="ribB"
                     /locus_tag="VFC43_06990"
     CDS             2567..3244
                     /gene="ribB"
                     /locus_tag="VFC43_06990"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HZK30959.1"
                     /translation="MIDDALAALREGKFILLYDFDDREGETDFAIRSDAVTPRHIVQM
                     RKDGGGLICTAVHPIAAQRLGLPFASDALRASAVAEREGDIPYDRKNHSSFSLWVNHR
                     NTFTGITDRDRSLTVNGIAEQVKKALNGGVVNFHEVFRTPGHMALLRAADGLLDQRKG
                     QTELSIALAKMAGVTPAITICEMLDDESGYALSKEDARLYARKHRLVFVEGPEVMERW
                     EQERSSR"
BASE COUNT          848 a          892 c          869 g          862 t
ORIGIN      
        1 tacgccgatt actgccacgt ctctcataac tgcatcacga tctttccctt atgaactgca
       61 tatctggcat agtcaagata tatcggattt tccagcaact ttttcacgcc gggggcagca
      121 tcacgcctga acttaccttg atggatcgta tcggtgacct caatgtcaaa tgcatcgctg
      181 cccgcgccgg aaccaaagga acagacaaat atcgtatctc ccggctttgc gatatcgagt
      241 gttgcggcga gtcctatggg agacgcgccc gaataagtat taccaagcca gggtacaacg
      301 agccccggtt tgatttgttc tgaagtgaat cccaggtttt ttgctgcctt ctgggggaac
      361 ttcgcatttg gctggtggaa gactgcatac gtgtaatctt tcgggctctt cccagtctgt
      421 tcgaataaga gcctgcttgc tccttcaaca tgtttgaaat agcccggctc tccggtgaat
      481 cgcccgccat gccggggata atcctgcccc tcacgtctcc agaagtctgg cgtatcagat
      541 gtaaatgagc aggtatggtg gatggttgca atgggatcat cccgcccgat gaggaaggca
      601 gcaccacccg ccgcggcggt gtattcgagg gcatcgcttg gagctccctg ggatacatca
      661 gaaccgatag caagcccata tgttatcatg ccgcttttta ccaggcccat acaggtctgt
      721 atagccgctg tacctgcctt gcaggcaaac tcaaaatctg cagcggtcat gtttggggtt
      781 gcgccaatcg cttctcccac cgtgcatgcg gtcggtttga ctgcgtaggg gtgagattca
      841 cttcccacat aaaccgctcc gatgtcttca ggattgatcg ttcggcggag taacgcgttc
      901 cgggcagctt caactgcaat tgtcgcagcg tcctcatcca ggtcggggac ggatttttca
      961 aaaacaccca gtcctcctga gatatctgct gcattagcac gccagactct cgcaatttct
     1021 tcgaccttaa tacggtatct cggtatatac acaccgtagg taatgatgcc taccattctt
     1081 tttccctcaa cgtactcaca attttgtcaa tttccatagt cgtgcacatc acggtgatgc
     1141 gatcccgttc tgccatcttc tccactattg ggtggacatc agcaatatca attccctgaa
     1201 gcaccacaac cctgggtttg aacggagtaa cacggattgc aaccatcggt gatttaccgg
     1261 ttgacacatt ggtaaaaacc actgcccgct ctgtgctcca cccataaatg cggttgaact
     1321 catcggatga gaactgcata atggcgttca ggctgttcac cacggtatat ccgaagagta
     1381 cctggtcaat ggacccgcag agcagtgtgc acccgatcgc ctcagagaaa tccttgagcg
     1441 gaaccgctgt ggcatattca tgaatgtcgt aaatcacatc atcctcaata ttggaaaaga
     1501 gcatagtgga aaatttctgg atatgttttc catcattttc ttcatccata tcaaggagcg
     1561 tatcgacaat tttgccaaca attgcggtgc cggggctttt tctcctgccg ctttcatagt
     1621 cgctgatcac tgaaggagat acacccagac gatcggcaag gatgccgggt gcgatctcaa
     1681 agttcacacg ccattttttc agcgcgtgac ctggtgagtc cgacagcgtg atctcacctg
     1741 ccattttctc ggcaagctgg ttccgcaatc cggatttcat gctcattaca aagaacccgg
     1801 agtattaaat aattaacgaa caggtattcg acagtgaacg aataacagta gagaaatgta
     1861 tggagagcaa cccataaata ataagcatct caattttgat aagcatgatt cccgctgaag
     1921 atctggagtg tctcaaggtg attgccctga ggggtggttg taaatgcccg gtttttgtct
     1981 cgtcgcagac gattggcgag atgctgggca tcagtcagca gacagcatcc cgcaggttaa
     2041 aagggctcga atcccagggt tttattacgc ggacaattgc accggatggt cagcatgtaa
     2101 cagtcaccag tactggtgaa gaggagctcc ggaaggaata ccaggcgtat tgccggattt
     2161 tttccgagcg caatatagga tatgttttaa ccggtactgt tgtcagcgga attggtgaag
     2221 gcaagtatta catgagcctt gaaccctata agcagcagtt taaaacactt cttggatttg
     2281 aaccataccc gggaacgctc aacatccgtc tccccgcttc ccgacttccg gtacgaaaga
     2341 agattgatgg tctcgactgg atacggataa agggattttc aaccgatggc aggacgtttg
     2401 gtgatgcaaa gtgcctgccg tgccgtattg gtgacattgc ctgtggcatt gtggttcccg
     2461 gcagtacgca ttacccggag gatattatag aagtgatcgc acccatcgcc ttacgacgga
     2521 aactgggtgt tgaggactca gataccgtta ctgtcgaggt cgggcaatga ttgatgatgc
     2581 cctggcagca cttcgggaag gcaaattcat cctcctctac gattttgacg accgcgaagg
     2641 ggagactgat tttgcaatcc ggtcagatgc agtaacaccc cgccacatag tgcagatgcg
     2701 caaggatggg ggcggtttga tctgcactgc cgttcacccg atcgcagccc agcgccttgg
     2761 actgcctttt gcaagtgacg cactgagagc cagtgcggtg gccgaacggg aaggtgacat
     2821 tccttacgac agaaagaacc actcctcgtt ctcgttatgg gtgaaccacc ggaatacctt
     2881 taccggtatt actgacaggg accgctcact aacggtaaat ggcattgcag aacaggtaaa
     2941 aaaggcgctc aatggtggag ttgtaaactt tcatgaagtc ttcaggactc ccgggcatat
     3001 ggctctcctg cgtgctgcgg atgggctgct cgaccagcgc aaaggtcaga ccgaactctc
     3061 aattgcgttg gcaaaaatgg cgggagtcac tccggcgatt acgatctgcg agatgctgga
     3121 cgatgaatcc ggctacgcac tttcaaaaga agatgcccgg ctctatgcac gcaaacaccg
     3181 gcttgtattt gtcgaaggcc ccgaagtaat ggagcggtgg gagcaggaac gttcctcacg
     3241 ctgatccatc ttcatctcat aaattccccg tgttctcctg gttagaaaag tatttcatta
     3301 ttcttattat ccattcaatc ccagttttat cagcatgaaa gtcccgctca ccgaacttac
     3361 caaccgtctg aaatgcttcc gggcaaggat ggacaagacg caaccgggct gggagatggc
     3421 agccattacc ggcaaggtac ccctctatta cttcacgggc accatgcagg a
//
LOCUS       DASRLV010000180         9294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1031939,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000180 DASRLV010000000
VERSION     DASRLV010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031939"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(286..843)
                     /locus_tag="VFC43_06995"
     CDS             complement(286..843)
                     /locus_tag="VFC43_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30960.1"
                     /translation="MAARQLVFITIVLIFLTLVAGCTDSSTEGTPLPTLSTLPVPASG
                     SQDIPLLQVFGETTGKGIPGGTIDTITFTIGLTDIYKSVDMERLTIVYADAIRTETLR
                     PVEGFHGDPPKGFWSITRVENEVGDPNNRLDNEERFVITINPKAPLVPRQILTIDIKP
                     QNGHSLTIRRVAPPSIFEDNILLPP"
     gene            993..2777
                     /locus_tag="VFC43_07000"
     CDS             993..2777
                     /locus_tag="VFC43_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30961.1"
                     /translation="MTLHFNENDYHLFYHYCANYCRLYDETRKNSIKILLEIYKHEVI
                     DIQEHSLDNIFSFFYQKKQEFETEGRVPASDSDNLMRIRICSDIIAFLDDLEKIYRLL
                     RKKNREANYILIIGVFSKIFEEEAQTRAEDLQKTASDAIDAIVSPLEKEIEKKLGTQA
                     DREAILYEMVSFTEIFNRLSPDLTFNDEVILLIAPKLLKRFGKDENYEEIRNLLKNHR
                     DQFGMDELESALEGGIHARNEWSLEFSWEDLLTPLRTLNDLVAQYQTPMRVSLNTLAA
                     LEAPMKGKPRQADYDQLTPYQKPYRAVMGKNQENQILVTIDNRSHSEVQLLSAPDSSH
                     QVNNQENKYKQYFSVTAGAMVILAILLASILLSFSSSPAFAVGNTTGGLTGAKNGTIP
                     SQVGTIHPSVVPVAIIGNEPAQIAISPPLLMPTPIPTRQYVTIEPVMRESGGPRGTRE
                     LYEQPASILGSLYDPDEYVTIFNNNMSYDETYYNISFDMKNPPMVIHYTVFAPNITDV
                     KWFAPRDPAKQIDTAVVIRPDESAWFELKIYDKDSLYDKQGWGREYGISSSTQEIFIR
                     NPGIYHLEFSGRLVTVNSEVLVKKEGNI"
     gene            2915..3826
                     /locus_tag="VFC43_07005"
     CDS             2915..3826
                     /locus_tag="VFC43_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30962.1"
                     /translation="MEIFTVIILILAGLVFCAIAGYLWVKRQGKTPSLSGVVIYQSQL
                     EPVIINHTSTNLSLVPESAIMNAKKTLHIAYGHTSHGSQITTGMTGLSRFAGAPHDSS
                     LYSWNSDEGKEALDLRDSVITGDLGNPDRRSWADRTRIYLNEHKDVNVVMWSWCGQVS
                     GAKESHINTYLSLMNTLESEYPGVKFVYMTGHLDGTGVTGNLHIRNEQIRNFCCANNK
                     ILYDFADIESYDPDGKFFLDKGADDGCNYERGTRNWAIDWQNSHRKGTDWFECSSAHS
                     VPLNANQKAYAAWWLWARLAGWDGTTR"
     gene            complement(4351..5091)
                     /locus_tag="VFC43_07010"
     CDS             complement(4351..5091)
                     /locus_tag="VFC43_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HZK30963.1"
                     /translation="MLPPGRTFMEQTRYENLTESDHARGLPAPPLEKPVPPTSRTIPL
                     PKPTDIIIPARELRTAIEQRRSIRHYSGDPLIIEELAYLCWCTQGIVEVKAPFYTLRN
                     VPSAGGRHALETYLLINRIKGIQPALYRYLPFSHTLITIDTSGGISDAVMSACLGQAF
                     VRSSAVTFIWSAVIDRMAWRYSERAYRLVHLDAGHVCQNLYLAAEQIGCGTCAIGAFD
                     DQEIADVLGIDGNEEFVLYCAAVGRKSE"
     gene            complement(5308..7050)
                     /locus_tag="VFC43_07015"
     CDS             complement(5308..7050)
                     /locus_tag="VFC43_07015"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HZK30964.1"
                     /translation="MVRNFNRWLIIAIIAILASGMVLTLWTVQREENLMRSDLLAKTR
                     LLQGGFTPGYVNTLTGTENDLASPDYQALKRQLIQLKSSNPEIRFLHIMGQKPDGTVF
                     FFIDSEPPESNDYSPPGQLYPEASPVLLNTFASGEATMGGPVTDRWGTWMSGLVPIRD
                     TNSGKVTAVLGTDIDSRDWNIQIIKASAPAVVAMLLLMLLMLILFYMQQRNAREKQIL
                     TTSEAAIRESENRYRKVFESTGTAMVIIEDDTTISLANHEFFRLTGYFPEDITRKLSW
                     TEIVFKDDLEKMMKEHHLRQEKPEEALKQYEFRLITKPGELHNILLTIDILPGTLKSV
                     GSLMDISEIKEIHAVLERHASELKRNADVLRQTNNKLNILNSITRHDILNQLTAILGY
                     LELTKMKYPDPSLQVFINKEIQAAETIKAQIMFTKDYQNIGVRSPQWFDLKKVILASA
                     ALLPLSTVKLDVDCDTLEIYADPMLEKVFYTLLENALRHAKTITTIEFSCCMLAEGLR
                     VIYQDNGEGIPAEYKEAIFEHEYFKHTGFGLFLSRAILDITGMKIAETGEPGKGARFE
                     IMVPVDAFRFTRRE"
     gene            complement(7156..7527)
                     /locus_tag="VFC43_07020"
     CDS             complement(7156..7527)
                     /locus_tag="VFC43_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZK30965.1"
                     /translation="MADADKKILIVENDEIVSKVLEWRITGLGYTVSGKTATGQGAIN
                     LTNKTRPDAILMDIGLDGSMDGIETAKIIQEKFNIPIIFLTAYSDTTILERIFPLKPA
                     NFITKPFTDDDLRVALRLSID"
     gene            complement(7527..>9294)
                     /locus_tag="VFC43_07025"
     CDS             complement(7527..>9294)
                     /locus_tag="VFC43_07025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZK30966.1"
                     /translation="VDEEGRIIVWNPAQEHITGISRTEAMGVYAWDLATRMLPEEHRR
                     AEIRSRMMESIQASLKSGKNNHTHPAYYHFHRPDGTKVTTKQTVFCIKTSRGHMIGTL
                     YQDVTEQRQADEKIVASEEKFRSLIENSLEPILILDFEGTVLLANKAAALLTETSDIV
                     AVVGRNVMEFIAPESQEDVIKDFAQVARGHDAYLAQYTVKTANGNKIPIESIGKRITY
                     EGRPADLISLRNITNRLEAETKLRDNESRLRSLIASMNDLVFVLDKDLVFREFYQHGT
                     EELFMKPEMFIGKRLDAVGFPEPARGIIQNALMQTLQTGNPAQAEYYLDFQKGRSWFD
                     LHVTAFQGSDGTRTGLTCVVRDSTDRKQAEESLQQVNKKLTLLSGITWHEINNQNIAL
                     DGYLSILEKKLTDPSLKEIFQKTTFATERIAGMIGFTREYEKIGIQAPTWQDCRTLIG
                     TAGQQAPLGHILLKNDLPAGSEIFADPLISLVIYNLLDNAVRYGGKISTLRIASHELD
                     GNRLIIFEDDGVGIPVEEKEKIFEYGFGKNTGMGLFLSREILGITGITIRETGKPGKG
                     ARFEVMVPKGMYREDEMKTGGS"
BASE COUNT         2411 a         2179 c         2281 g         2423 t
ORIGIN      
        1 tccatatccg atcccatgaa ctgatgggaa tatgaacacg agccgggcga agtgcgaaag
       61 tgcccgggtg gcttaatagg atccagcggc accgccaatg gatgatgaca gaagcaaaaa
      121 aacaggaata aatccaataa aaaacagcca ggaattccgt agctgttgaa aatccaggga
      181 ataaacgtga ctctggcagt atgagaatcc aaaatactgc ttacagttca atggtttcct
      241 gatataggat cgttttagtg cagcgccgga tcgtgatatc agggattacg ggggtaaaag
      301 gatattatct tcgaaaattg acggtggagc gacccggcgg atggtgaggg aatgaccgtt
      361 ctggggtttt atatcgatag tgaggatctg ccgggggaca aggggtgcct ttgggttgat
      421 ggtgatgaca aaccgctctt cattatcgag gcggttgttg ggatcgccca cctcgttttc
      481 cacgcgggtg atgctccaga acccctttgg tggatcgcca tggaaaccct cgaccgggcg
      541 aagggtctcg gtccggatgg catctgcata gacaatggta agacgttcca tatccacgga
      601 tttgtagata tcagtaaggc cgatcgtgaa cgtgattgtg tcaatggtgc cgccgggtat
      661 accttttccg gtcgtttcac cgaatacctg gaggagtgga atgtcctggg aaccggaggc
      721 cggtactggc agggtactta aagtcgggag tggcgtgccc tcagttgagg aatcggtgca
      781 gcccgctaca agagtgagga aaataagcac aatcgttatg aaaactaact gccgcgctgc
      841 catagggaga ccacactgtg atcttgtggt agaaaccatg ggtcagtgga ctagaaaaac
      901 aattgggtag cagcatacag gattaaatat tcgttattga cgaatgagta aaataaaata
      961 cgaaggacta ttgattagta cacatgggaa gagtgacgtt acatttcaat gaaaatgact
     1021 accatctctt ctaccactac tgcgccaatt attgtcgtct ttatgacgaa acaagaaaaa
     1081 attcaataaa aattcttctc gagatctata aacacgaagt cattgatatc caggaacatt
     1141 cccttgacaa tattttttct tttttttacc agaaaaaaca ggaatttgag accgaaggac
     1201 gcgtacctgc cagtgattca gacaacctga tgcggatccg gatatgttct gacatcatcg
     1261 cttttttaga tgaccttgaa aaaatatacc gtctattaag gaaaaaaaac agggaagcaa
     1321 attatatcct gatcatcggt gtattttcaa agatttttga agaagaagcc cagacccggg
     1381 cagaagatct tcaaaaaacc gcatctgatg ccatcgatgc catcgtttca cccctggaaa
     1441 aagaaataga aaaaaaactc ggcacacagg cggacagaga ggcaatcctc tatgaaatgg
     1501 taagttttac agaaattttt aacaggcttt caccggatct tacctttaat gatgaagtca
     1561 tcctgctcat cgcacccaaa ttactcaaaa ggtttgggaa agatgaaaac tacgaagaaa
     1621 tccggaattt attaaagaac caccgtgatc aattcgggat ggatgaactc gagtcagcac
     1681 tggagggagg tatccatgca cgaaacgagt ggagtcttga attttcatgg gaggacctcc
     1741 tgacaccact tcgtacactc aatgacctgg ttgcacagta ccagaccccc atgcgggtgt
     1801 ctctcaatac gctcgcagca ctcgaagcgc ccatgaaggg aaaaccccgt caggcagatt
     1861 atgatcagct gacgccgtat cagaaaccct atagggcggt aatggggaag aaccaggaaa
     1921 accagatcct tgtaacaatt gataaccgtt ctcacagtga ggttcagctc ttatcggcac
     1981 cggactcaag ccaccaggta aacaatcagg aaaataagta taaacaatac ttttccgtta
     2041 ctgcaggggc aatggtgatc ctcgcaatac tattagccag cattctcctt tctttctctt
     2101 cttctcctgc gtttgctgtc gggaacacaa ccggaggact gactggtgcg aaaaatggaa
     2161 cgataccctc tcaggtgggc acgattcacc catctgttgt accagttgca ataatcggga
     2221 acgagcccgc tcagatagcg atatctcccc ccctcctgat gccgacaccg atcccgacac
     2281 gacagtacgt aacgattgaa cccgttatgc gggaatccgg tggtccccgg ggcaccaggg
     2341 aattgtacga acagccggca tccatcctgg gtagtttata cgatccggat gaatatgtaa
     2401 ccattttcaa caataacatg tcgtatgatg agacatacta caacatttcc tttgatatga
     2461 aaaatcctcc tatggtaatc cactataccg tctttgcacc aaatatcact gatgtgaaat
     2521 ggtttgcacc acgggaccct gcaaagcaga ttgatacagc cgtggtgatc cgccctgatg
     2581 agtcggcatg gtttgaatta aaaatctatg ataaagatag cctgtatgat aagcagggtt
     2641 ggggaagaga gtatggtatt tcttcatcaa cacaggaaat ttttatcagg aatccgggaa
     2701 tataccatct ggagttttcc ggtcgcctgg tgaccgttaa ttcagaagta ctggtgaaaa
     2761 aagaaggaaa tatttaggtc tggttccctg aaccctgccg atttttttaa acgggaatct
     2821 ctctttgatc actccgggga tcttttacaa aagaaccgaa ctataaatag accggaacat
     2881 aaaaaatgag agagatcagc aggtcgggaa aagaatggag atatttacgg ttattatcct
     2941 tatcctggca ggattggtgt tttgtgcaat tgcaggatat ctgtgggtga aaagacaggg
     3001 caaaaccccc tccctttcag gagttgtcat ataccaaagc cagctggaac cggtgattat
     3061 caaccatacc agcacgaatc tgtcccttgt tccggaatct gctataatga atgcgaaaaa
     3121 aacgctgcat atcgcatacg gacatacgtc ccacggcagc cagatcacca ccggaatgac
     3181 ggggctttcc cgattcgccg gagctcccca tgacagttcc ctgtattcct ggaacagcga
     3241 tgagggaaaa gaagcacttg acttacggga cagtgttata accggtgacc tgggaaatcc
     3301 tgaccgcaga tcatgggcgg acaggaccag gatatacctg aacgagcata aggatgttaa
     3361 tgtggtcatg tggtcatggt gcgggcaggt atccggtgca aaggagtccc atatcaatac
     3421 atatctttcc ctgatgaaca cgcttgaatc agaatacccg ggtgtgaagt ttgtctatat
     3481 gaccgggcac ctggatggga ctggcgttac cggtaacctg catatcagga atgaacagat
     3541 acggaatttc tgctgtgcaa acaataagat tctctatgat tttgctgata tcgagagtta
     3601 tgatcctgat ggaaaatttt ttcttgataa gggtgcggat gacgggtgta attatgaaag
     3661 gggtacccgc aactgggcaa tcgactggca gaacagccat cgaaagggaa ctgactggtt
     3721 tgaatgcagc tctgcccatt ctgtgcccct gaatgctaac cagaaagcat atgccgcatg
     3781 gtggctgtgg gcaaggcttg ccggctggga cggtactacc cggtaaaata cccttataaa
     3841 aaaggggaag cctcttttta ctaattcgga atcatatgaa cagataactt tttacctact
     3901 ggaaatggta tcgtgccgtt tcgcatccaa agcccggccc agatccaacc tgaaccgggt
     3961 gatttaaaaa tcgcatataa agccgtcctg ctgcaaatgg gggtttatat acagtccgct
     4021 ccagtggaag aaaaggggta taaaaggatg atcaatttgc cggattggtt aaatatgcaa
     4081 aatatattta taaattgtta aaatagtggg aaaaacgggg gaattttccc cgtcataata
     4141 tgcatctttt ccctgccgga caaagatgac ccgagagcaa tccattgtta aaatgtatga
     4201 atgccagaca tcatcatagt ctggtacttt tgtgaatccc gggagggaat cctgcttcaa
     4261 tccgccccga cagggtatca acaggcattg gcagcgaacg atatagtatt catcccgcgt
     4321 ccggaagttt tcatcagttc ctgcacggtc tcattcactc ttcctcccta cggccgcaca
     4381 gtagagtaca aactcctcgt ttccatcaat tccgagcaca tctgcgattt cctggtcatc
     4441 aaatgccccg attgcgcagg taccacaacc gatctgctct gcggcaagat agaggttctg
     4501 gcagacatgc cctgcatcga gatgaacgag acggtacgcc cgttcactgt atctccatgc
     4561 catccggtcg atcactgcag accagatgaa cgtaaccgct gaagatcgca cgaaggcctg
     4621 tccgagacat gcagacatta ccgcatcgga aatcccgccg gatgtgtcaa tggtgatgag
     4681 tgtatgggag aagggtaggt accggtacag cgcgggttgt attcctttga tccggttgat
     4741 gaggagatag gtctccaaag cgtgccgccc accggcagaa gggacgttcc tgagggtata
     4801 gaacggagct ttcacttcaa caatcccctg cgtacaccag cagaggtagg caagttcttc
     4861 gatgataagg ggatcacccg aatagtgacg gatggaacgg cgttgctcaa tcgcagtacg
     4921 gagttcccgt gctggtatga tgatgtctgt tggtttggga agcgggattg tgcgtgaagt
     4981 cggcggaacg ggtttttcca gcgggggagc gggaagcccc cgtgcatggt cagattcggt
     5041 caggttttca taccgggtct gttccatgaa tgtgcgaccg ggtggcagca taagcctgtg
     5101 attgtcgctt catatgaata aactgacctg aggagtaatg atgtaatatc acactatggc
     5161 ttcacgaaca gccgggtata cttgtccatg ggtgcatttc ccggatttca atgatcgcaa
     5221 caacaaaatc accaactagg aattttaccg gtcccccatt gagcaaaatt gagatatcca
     5281 aggtcgccct tgctcaattt tgaattttta ttcacgtctg gtaaaccgga aagcatccac
     5341 gggtaccata atctcaaacc gtgcaccttt ccccggctct ccggtttcag cgatcttcat
     5401 accggtgatg tccagaatag cacgcgaaag gaataaccca aagccggtgt gcttgaaata
     5461 ctcgtgttcg aaaattgctt ccttatactc agcggggata ccctctccat tgtcctggta
     5521 tattacccta agtccctctg ccagcataca acaggaaaat tcgatggttg taatcgtttt
     5581 agcatggcgg agtgcgtttt caagcagagt gtagaagacc ttttcaagca tcggatctgc
     5641 ataaatctca agggtatcac agtccacgtc cagtttcacc gtggaaagcg ggagaagagc
     5701 tgcactggcc agaatgacct ttttgagatc aaaccattgc ggggaccgga ccccgatatt
     5761 ctggtaatcc ttggtgaaca tgatctgggc cttgatggtt tcagcggcct gaatctcttt
     5821 attgatgaat acttggaggg agggatccgg atatttcatt ttcgttaatt ccagataccc
     5881 gagaatagcc gtcagctggt tgaggatgtc gtggcgtgtt atactgttta agatgttcag
     5941 cttattgttg gtttgccgga gaacatccgc attacgtttc agctctgaag catgccgttc
     6001 cagtacagcg tgaatctcct ttatttcaga aatatccatc agtgatccga cgctttttaa
     6061 ggtaccgggc aatatgtcga tggtcagcag gatgttgtgt agttcccccg gttttgtgat
     6121 aagccggaac tcgtattgtt tcaaagcctc ttccggcttt tcctggcgta aatgatgctc
     6181 cttcatcatc ttttcgagat cgtctttgaa cacgatttct gtccaggaca acttacgggt
     6241 gatatcctcc gggaaatagc cggtcagccg gaaaaactcg tgattggcaa ggctgatagt
     6301 ggtatcgtct tcgatgatga ccattgccgt gccggtgctt tcaaagacct tcctgtaccg
     6361 gttttcactc tcccgtatgg cagcttcaga ggtagttagg atctgtttct cccgggcatt
     6421 tcgttgctgc atatagaata atatcagcat gagcagcatg agcaggagca tggcaacgac
     6481 ggctggtgca ctggccttga taatctgtat gttccagtcc cgggaatcaa tatctgttcc
     6541 taatacagct gtcaccttac cactattggt atcccgtatc ggcacgagcc cactcatcca
     6601 tgttccccag cgatcggtca ccgggcctcc catggttgcc tctcctgaag caaaagtatt
     6661 cagcaggaca ggagacgcct caggatacaa ttggccgggg ggcgagtaat cgttcgattc
     6721 cggaggttct gagtcaataa agaaaaaaac cgtcccgtcc ggcttttgtc ccatgatgtg
     6781 gaggaaccgg atctcaggat tggaggattt gagctgtatc agttgtcttt ttaatgcctg
     6841 gtaatccgga gatgccagat cattttcagt tcctgttaac gtgttcacgt aaccgggagt
     6901 gaaaccgccc tgcaaaagac gggtttttgc aaggagatcg gaacgcatga ggttttcttc
     6961 ccgctgaacc gtccacaggg tcagaaccat accactggca agtatggcga tgattgcgat
     7021 gatcagccat ctgttgaaat tgcgaaccat tcttcaacct tctcctggat ccctgtatcg
     7081 catcacttca gcatcgtgtc cgtttttttg tctgcatttt tgaaaatgac ccgaatgaat
     7141 ataatcttat cataatcaat cgatcgataa tctgagtgcg acccgcaggt catcatcagt
     7201 aaatggtttg gtgataaagt ttgcaggttt gagggggaat atcctttcaa ggatggtagt
     7261 atcagaatac gcagtaagga atatgatcgg aatattgaat ttttcctgaa tgattttagc
     7321 ggtttcaatg ccatccatcg acccgtccag accaatgtcc atgagaatgg catcaggtct
     7381 ggtcttattt gtcaggttaa ttgcaccctg accagtagct gttttaccac tgactgtata
     7441 ccccagcccg gtgattcgcc attcgagcac tttactgacg atttcgtcat tctccactat
     7501 caggattttt ttatctgcat ccgccattaa ctccctccgg ttttcatctc atcctctcgg
     7561 tacatcccct tcgggaccat aacttcaaac ctcgctcctt tgcccggttt cccggtctcc
     7621 cggatggtga tgccggttat cccgaggatc tcccgggaaa ggaagagacc catgccggta
     7681 tttttcccaa agccatattc gaagatcttc tccttttctt caaccggtat tccaacacca
     7741 tcgtcttcga agatgatgag ccggttcccg tcaagctcgt gtgaggcaat ccggagagtt
     7801 gaaatctttc caccgtaccg cacagcgttg tccagcaggt tatatatgac taaggagata
     7861 agcggatcgg caaagatttc agaaccggcc gggagatcat ttttcagcag tatgtgtccg
     7921 aggggggctt gttgtcctgc agtcccgatg agagtccggc agtcctgcca ggtcggggct
     7981 tgtattccga tcttttcata ttctctggtg aagccgatca tcccggcgat tcgttcggtg
     8041 gcaaacgtag tcttctggaa aatctcctta agtgagggat ccgtaagctt cttttcgaga
     8101 atgctaagat atccatcaag ggctatgttc tggttgttta tctcgtgcca ggtgatgccg
     8161 gagagcaggg tgagcttctt gtttacctgt tgtaatgact cttcggcctg cttgcgatcg
     8221 gtactgtcgc gcacaacaca ggtcaaaccg gttcgtgtac cgtcactgcc ctgaaacgcg
     8281 gtgacatgaa ggtcgaacca tgatcgtcct ttttgaaaat ccagataata ttctgcctgg
     8341 gcagggtttc cggtctgcag ggtctgcatc agtgcgtttt gaatgattcc ccgtgccggc
     8401 tccggaaaac cgaccgcatc aagccgcttg ccaataaaca tttccggttt catgaacagc
     8461 tcttcggtgc catgctggta aaattccctg aaaacaagat ccttgtccaa aacaaagaca
     8521 aggtcgttca tactcgcgat gagcgatctc agccggctct cattatcccg cagttttgtc
     8581 tcggcttcca gacgatttgt gatgttccgg agggagatga ggtccgcggg cctgccttca
     8641 tacgttatgc gcttgccaat ggattcaatg gggattttgt tcccgtttgc cgtttttacg
     8701 gtgtattgtg caagatatgc atcgtggccc cgggcgacct gcgcaaagtc cttaatcacg
     8761 tcttcctgtg attccggtgc aatgaactcc atcacattcc tgccgacaac tgcaacaata
     8821 tcgctggttt ctgtaaggag ggcagctgcc ttgttggcca gcaggaccgt cccctcgaaa
     8881 tcgaggatga ggatcggttc aagggaattt tcaataagtg atcggaactt ctcctcgctc
     8941 gccacaatct tctcatctgc ctgcctttgt tcagtaacgt cctgatacag ggtaccgatc
     9001 atgtggcctc gtgaggtttt tatgcaaaac accgtctgtt tggtggtaac tttggttcca
     9061 tcaggtctgt gaaaatgata atacgccgga tgcgtatggt tattcttccc ggattttaga
     9121 gatgcctgga tactttccat cattctggag cggatctccg cacggcggtg ttcttctggt
     9181 aacatccggg ttgcaagatc ccaggcataa acccccatcg cttcagtccg ggagatcccg
     9241 gttatgtgtt cctgtgcagg gttccataca attatccgtc cctcttcatc caca
//
LOCUS       DASRLV010000181         5620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_226859,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000181 DASRLV010000000
VERSION     DASRLV010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226859"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..88)
                     /locus_tag="VFC43_07030"
     tRNA            complement(16..88)
                     /locus_tag="VFC43_07030"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(53..55),aa:Asn,seq:gtt)
     gene            complement(215..499)
                     /locus_tag="VFC43_07035"
     CDS             complement(215..499)
                     /locus_tag="VFC43_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30967.1"
                     /translation="MLRKMKDEIELLSRHIEVAKAVEDHQPIGIMKLAELLNLPSHRI
                     RYSLHVLEQLGYIRASSEGAVATALTKELFAHLNDDLDELVRLLEMMKRD"
     gene            complement(531..803)
                     /locus_tag="VFC43_07040"
     CDS             complement(531..803)
                     /locus_tag="VFC43_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30968.1"
                     /translation="MDEDAKNRFKWKFYRLTIQLNIIILLIALSVIVFLIIHSPYSVP
                     VIICMIFLALFLALDFIKKYKETKKWLGEQPQKDKKENDTRQDDII"
     gene            864..1991
                     /locus_tag="VFC43_07045"
     CDS             864..1991
                     /locus_tag="VFC43_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HZK30969.1"
                     /translation="MDQLSNAIKKSSTDAYVIYASSRDADMRYLTHFTTSDPFIFFKK
                     PGDPGVIIVSQMELGRASREATTAVMTRTHAGLPDILKEEKDPYRATARMIAGQVGTK
                     ILVPPNFPVALANALSEHCTLSVDKGTVMDMRSKKSRHEILVMRNVQKVTETAMERAI
                     SLIKSASQKKGILHLDEKPLTAEQIKFSMHSLLLQYGCGAVDTIVSCGEDTATPHISG
                     SGPLKSDEPIVIDLFPVEEMSGYYADMTRTVVRGEPSTEILEMYDTLREAKHLGTSLV
                     KTGVETADIHLAVVDFFKDHGYESNTRGFVHNLGHGVGLQVHELPSVGPAGKALASGN
                     VITIEPGLYYPGIGGVRLEDIGAVTTRGFDNFTIFPEDLVV"
     gene            1988..2869
                     /gene="map"
                     /locus_tag="VFC43_07050"
     CDS             1988..2869
                     /gene="map"
                     /locus_tag="VFC43_07050"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II methionyl aminopeptidase"
                     /protein_id="HZK30970.1"
                     /translation="MKEEIFEKYRDAGEIAAKILRLGSQDIREGVSYLEVVETIELQV
                     REEGAELAFPLNLSLNEDAAHDTASPGDARVFARGDVAKLDLGVQIDGYIADTATTID
                     LGNNSLLLEASQEALDAAIKAVRPGTTAGEIGAAVQKAIEYRGYRPIANLTGHGLDQY
                     ILHRPPTIPNVGVNGGVILEEGMVFAIEPFATTGSGHVGEKTRKEIYSQLSQKPVRIP
                     AARAILSKVKDRHGLPFARRWLDEKKLDITLPALVRSQVLHVYPVLSDIPGSLVSQHE
                     HTVIVTNDGCIVTTRSR"
     gene            2909..4183
                     /locus_tag="VFC43_07055"
     CDS             2909..4183
                     /locus_tag="VFC43_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK30971.1"
                     /translation="MSGDSEILRIMEVVLTAEIFNQNPQLDINDLPPFCREIFSITTS
                     ADVKRPVYVSDGLIKRSLAIADAHQKLSANPFVAYEEFGQRIRITALEPAALWFLKQG
                     GQPIIDKNPTLAFFFENIDSTGIDYKTVRKANPPFEDTKAYLDARVSKLIGEDEKLRG
                     ALDLVIILAPEEVEQQMDDLVCTPEQTAIIAKIQLAVDHREFLHRHRIHEVGKLLFVG
                     PPGTGKTSLALAMSRTMHMPVLEVRLSMVTSQYLGETSKNIDRIFELAKKLAPSILFI
                     DEFDFVAKSRVADDHGAMKRAVNSLLKNIDKINLVRNNVLLIGATNHPQLLDEAAWRR
                     FDEVVEFSLPNEEMRKNILMKVTSSFQCTLDYQQLASQTDGFSGSDLRMMVKEAILSS
                     LMANRKIIAREDIEKGIHMVKNRDAIRHLTWI"
     gene            4185..4907
                     /locus_tag="VFC43_07060"
     CDS             4185..4907
                     /locus_tag="VFC43_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZK30972.1"
                     /translation="MKITLLGTGDAIGTPKVGCTCPQCTHAKKTGAMRLRTSLLIENE
                     ERHILIDSSPDLRQQLLLHGSPHIDAVIWTHGHYDHFIGFGEFYRIQKIPPVFAAPQV
                     LNYCAETFRFLTFDKGEVQPYEPFDIFGLTITLFMVKHPPAFTCGIVFETADSKVGFT
                     SDTNIDLPQKSLDLLTNLDLLLLDALVPSDITIHKHMNYLDACTLAQSLAPKEYRCIH
                     QSHLIPWDLPHLGKDGDVFEFR"
     gene            5010..5279
                     /locus_tag="VFC43_07065"
     CDS             5010..5279
                     /locus_tag="VFC43_07065"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit L"
                     /protein_id="HZK30973.1"
                     /translation="MIVKVLDLEKDKVRLIIKGEGHTFMNALVEELLNDPDVDVAKYM
                     IEFQFSDPELLVTTKGKKNPLPIIKKACRRISGHCNELIKDLKKN"
BASE COUNT         1543 a         1361 c         1273 g         1443 t
ORIGIN      
        1 ctacgggccc aaaaacgccc cccacagggc tcgaacctgt gacattcgga ttaacagtcc
       61 gacactctac caactgagtt agggaggcag tctcaatgcg ccttattaga ttctttctga
      121 gtgcttttaa aagtaactat ggatgatcga tccctttttc aggagaggga cttcgggaga
      181 gagatggatc aagggagagg gagttacggc cgcttcaatc gcgcttcatc atttcaagca
      241 ggcgaaccag ttcatccaga tcatcgttga gatgtgcaaa caattctttt gtcaaggcag
      301 tcgcgacagc tccctcggaa gatgcacgga tatagccaag ctgttccagt acatgaaggg
      361 agtaacggat ccggtgagag ggaagattga gcaactcggc cagtttcatg ataccaatcg
      421 gctggtgatc ttccactgct tttgcaactt caatgtgacg gcttaacagt tcgatctcgt
      481 ctttcatctt cctgagcata gtactcccat aaatccctca tcccgcgcta ttatattata
      541 tcgtcctgtc tggtatcatt ctctttttta tctttttgcg gttgctcacc gagccacttt
      601 tttgtttctt tgtatttctt aataaaatcc aaagcaagaa agagagccag aaagatcatg
      661 cagatgatta cgggaacact atagggggaa tgaataatta aaaacacaat taccgataaa
      721 gctatgagga gaataatgat atttagctgt attgtgagcc ggtaaaattt ccatttgaac
      781 cggttttttg catcctcatc cattgctggt atgtcacctc cgaaataaaa gtacttgacg
      841 tttaactaca tatatcctga tagatggacc aactttcaaa cgcgatcaaa aaaagcagta
      901 ctgatgccta cgtaatatat gcatcttcgc gtgatgccga tatgaggtac ctgacccatt
      961 ttacgaccag cgatcccttc atctttttta aaaaacccgg agatcccggc gtaattatcg
     1021 tatcccagat ggagctcggg cgcgcatcac gagaagccac tacggctgtg atgaccagaa
     1081 cccatgccgg gctcccagac atcctaaaag aagaaaagga tccataccgg gcaacagcga
     1141 ggatgattgc cggtcaggtc ggaacaaaaa tcctcgttcc ccctaatttc ccggttgctt
     1201 tggcaaatgc cctgagtgag cattgtaccc tatccgtgga caaaggaacg gtcatggata
     1261 tgcggtcaaa gaaaagcaga catgaaattc tggtgatgag aaatgtgcag aaagtgacgg
     1321 aaacagcgat ggagcgtgca atctcactga tcaagagcgc atcccaaaag aaaggaattc
     1381 tccatcttga tgaaaaacca ttaaccgccg aacagataaa attctccatg cacagtctgc
     1441 tcctgcaata cgggtgcggc gcagtggata ctattgtttc atgcggagag gataccgcta
     1501 caccccacat atcaggctcc gggccgctca aatcagatga accaattgta attgacctct
     1561 tccccgtaga agagatgtca ggctattacg cggatatgac ccggaccgtt gtcagggggg
     1621 aaccttcaac agagatcctc gagatgtacg atacactccg tgaagcaaaa catcttggga
     1681 catctctcgt aaaaacagga gtggagactg cagatattca tcttgcagtg gttgactttt
     1741 tcaaagacca tggatacgag agcaacacac gaggatttgt acacaatctc ggtcatgggg
     1801 tgggtcttca ggtccacgag cttccctctg taggacctgc gggaaaagca ctcgcctccg
     1861 gcaatgtaat caccatcgaa ccgggactct actatcccgg catcggcggc gtgcgtcttg
     1921 aggatattgg cgcagtaact acacgggggt ttgataactt tacgatattt cccgaggatc
     1981 tggtcgtatg aaggaagaga tttttgagaa atatcgggat gccggagaga tcgccgctaa
     2041 aatcctgcgc ctgggttcgc aggatattcg cgaaggtgta tcttatcttg aagtcgttga
     2101 aaccattgaa cttcaggtga gagaagaggg ggctgaactc gcttttcccc tcaacctttc
     2161 cttaaatgaa gatgcggcac acgacactgc atcaccagga gatgcacggg tatttgcccg
     2221 gggcgatgtg gcaaaactgg accttggcgt gcagatcgac gggtacatcg ctgacacggc
     2281 gactactatt gatctgggaa acaactcact tctccttgag gcttctcaag aagcgctcga
     2341 tgctgcaata aaagcagtcc gcccgggaac gaccgccgga gagattgggg cagctgtcca
     2401 gaaggcaatt gagtaccggg gttatcgccc tatagcaaac cttaccgggc atggacttga
     2461 tcagtatatt ctccaccgcc cccccactat ccctaatgtc ggggtcaacg gcggggtaat
     2521 actggaggag gggatggtct ttgccattga acctttcgca acaaccggga gcgggcatgt
     2581 cggagaaaag acacgcaagg aaatttattc gcagctatca caaaagcccg tacgaatccc
     2641 ggctgcacgt gctatcctga gtaaagtgaa ggatcgtcac gggctcccgt ttgcccggag
     2701 gtggcttgat gaaaaaaaac tggatatcac cctccctgct ctggtgagat cgcaggtcct
     2761 ccatgtttat ccggtccttt ctgacatacc gggatccctc gtttcccagc atgagcatac
     2821 ggtaattgtg acgaacgatg gctgtatcgt gactacacgc tcaagatagt cttattcatt
     2881 caggagatga aaagataagg ttacagccat gtctggagat tctgaaattc tgcgaataat
     2941 ggaagttgtt ctgaccgctg aaatattcaa tcagaacccc cagcttgata tcaatgatct
     3001 gcctccattc tgccgtgaga ttttttcaat caccacttcc gcagatgtca aacggccggt
     3061 ctatgtaagt gacggcctta tcaagcgctc actagctatt gcagatgccc accagaaact
     3121 atctgcaaac ccctttgtcg cttacgagga gttcggacaa cgcatccgta tcaccgctct
     3181 tgaacccgca gcactgtggt tcctcaaaca gggaggccaa ccaatcattg ataagaatcc
     3241 tacgctcgcc ttcttcttcg agaatattga ttcgacaggg attgactaca agaccgttcg
     3301 caaagcaaat ccgccatttg aagatactaa ggcttacctc gatgcccggg tatcaaaact
     3361 aattggggaa gatgaaaaac tccggggtgc actagacctt gtcattatcc tagcccccga
     3421 agaagtggaa cagcagatgg atgaccttgt ctgtacaccg gaacagaccg ctattatcgc
     3481 aaaaatccag cttgcggtag accacaggga attcctgcac cggcacagga tccatgaggt
     3541 aggaaaactt ctctttgtcg gtccacctgg aacaggaaag acttcacttg cccttgccat
     3601 gtcccggacc atgcatatgc cggttcttga agtcaggctc tcaatggtta catcccaata
     3661 cctcggggag acttctaaaa atattgaccg catctttgaa ttagccaaaa aacttgcacc
     3721 ttccattctt tttatcgatg aattcgattt tgttgcaaag agtcgtgtcg ccgatgacca
     3781 tggagcgatg aaacgtgcag tcaattccct tttaaaaaac atcgataaga tcaaccttgt
     3841 aagaaacaat gtgctcctga ttggcgcaac caatcatcca caactgctcg atgaggcagc
     3901 atggcgaagg tttgatgaag ttgtggagtt ctcacttcca aatgaagaga tgcggaagaa
     3961 cattctgatg aaggttactt cctctttcca gtgtacactc gattatcagc agctggcttc
     4021 ccagacagat gggttttccg gctccgatct ccgcatgatg gtcaaggagg caattctttc
     4081 ttctctcatg gccaaccgta agattatcgc gcgcgaagat atcgagaagg gtattcatat
     4141 ggtgaaaaac cgggatgcaa tccgccacct gacctggata taacatgaag atcacacttc
     4201 tgggaaccgg tgatgcaatt ggtacaccaa aggtgggatg cacctgtccc cagtgcaccc
     4261 atgcgaaaaa aacaggtgcg atgcgtctta gaacttcact tcttatagag aatgaggaga
     4321 gacacatcct tatcgattca tctccggatc tcaggcaaca gcttcttttg catggctctc
     4381 cacatatcga cgctgtgatc tggactcatg gacactatga tcatttcata ggatttggtg
     4441 agttctaccg gattcagaaa attccccccg tatttgctgc tcctcaggtc ctcaattact
     4501 gtgctgaaac attccggttt cttacctttg ataaaggaga ggttcaaccc tatgagccct
     4561 ttgatatatt cggccttacg atcacgctgt tcatggtaaa acatcccccg gcatttacct
     4621 gtggcatagt ttttgaaacc gcggactcaa aggttgggtt tacttcagat acaaacatcg
     4681 atctcccgca aaaaagcctt gatcttctca cgaatctgga tcttctgcta ctcgatgcac
     4741 ttgtgccttc cgatataacc attcacaagc acatgaatta tcttgacgcc tgtactcttg
     4801 cccagtccct cgctccaaaa gagtatcgct gcatacacca gagccacctt atcccttggg
     4861 atcttcccca tctcggaaaa gatggagacg tgtttgagtt ccggtaaata tatcggtttt
     4921 ataaagccga tccgggtttt ccacaaaaat attttggtgt ccagtattac ccatacttta
     4981 atagttttca ggtgcccaaa gataggtgca tgattgtaaa agttctcgat ctcgaaaagg
     5041 acaaagtgcg gcttatcatc aagggggaag ggcatacgtt catgaatgcc cttgttgaag
     5101 agcttttaaa cgacccggac gtcgatgtag ccaagtacat gattgagttc cagttttcag
     5161 atccggaact tctcgtgacc acaaaaggca agaaaaatcc cctgcccatt atcaaaaagg
     5221 catgcaggcg tatatccgga cactgcaatg agcttattaa agatctgaag aaaaactgat
     5281 ttgaaacctt cctgatggat atactttttt taaaaagcga actatttaac ccgattgtct
     5341 gtggccctaa ttttcagact tggcagcaag ccactttttc aagttcccaa actgcttata
     5401 ccttttttcc aatcattcgc aaaagacctg tgttcgtcaa ttcagatttt ttcgccatct
     5461 gttaaggcaa tagaacataa gattttcttc aaaaaagcct gtaaaaaaaa gaccataaat
     5521 ttaaaagaaa ttattcttcc gttgcccgtt cggtgaatac aatcgatccc gaaatccgtg
     5581 agattttgat ttttaccttc tgcccgggct ttgcatttgc
//
LOCUS       DASRLV010000182         3476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_708244,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000182 DASRLV010000000
VERSION     DASRLV010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708244"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VFC43_07070"
     CDS             complement(<1..673)
                     /locus_tag="VFC43_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF63 family protein"
                     /protein_id="HZK30974.1"
                     /translation="MISDFIYKYYIDPIRLGQSYNIVDTLTYAVILVIGVYLLYRWMS
                     QSTYLSDSGFKIDANFILATLPYVVLGGVLRVVQDTGMIKSDFQFLLVTPLIYFVLFF
                     FTIAMLFLSRSITLQGLTKNVLTFYMFAGIVSTIVVSLVLLAWGINHAHVDLFILAII
                     PLMAITATLLVWAFMRYLLCWNYVNDPLYLALLFGQLLDASATSYGIDLHPSVQYIEQ
                     HVVGSG"
     gene            685..1764
                     /locus_tag="VFC43_07075"
     CDS             685..1764
                     /locus_tag="VFC43_07075"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-1-phosphate
                     dehydrogenase"
                     /protein_id="HZK30975.1"
                     /translation="MSADAIKLLKPKIFDKSKWMQLPRDVVIGHDVFGQIPSVCDDLK
                     LGSSALLISGKGTMGLAGNKVLDILKKDYTVKPFIAEEISIPIIKKAQKAAAGMDFVL
                     GIGGGRVIDTAKIVSYNLDLQFISIPTAASHDGIASSRASVPTVEGHASLDAQPPIAI
                     VADTGIIASAPHRLLAAGCADVISNYTAILDWELAHRVKGEPMSEYAIALSKMTAEIL
                     VKNAHLIKPHQEQSAWFVTKALVSSGVAMSIAGSSRPASGGEHKFSHALDRLAPGKAL
                     HGESCGIGTIISMYLHGGDWRGIRASLKCIGAPVTPADVGIDDGIAVEALLMAKAIRP
                     ERFTILDMGLTRESAENLIQMLYRE"
     gene            1776..2222
                     /locus_tag="VFC43_07080"
     CDS             1776..2222
                     /locus_tag="VFC43_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0179 family protein"
                     /protein_id="HZK30976.1"
                     /translation="MAEAKTKVTLVGTVLAKQGVDFIYEGEVAGCDTCKVKKACNNLQ
                     KGRKYRIVSVRTTHHECMVHFNGATAVEVIDAPVTALINADMAIINSKIKLDLSCNRN
                     DCRSFPLCHPDGVVEGEKYIVFTILGNAPDICEKGRALKLVDIKPA"
     gene            2521..3273
                     /locus_tag="VFC43_07085"
     CDS             2521..3273
                     /locus_tag="VFC43_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30977.1"
                     /translation="MKEDTRWKLVAGIGLVTLSLALFTAHYLIFQDAHHLFIFFIGDI
                     AFIPIEVLIVTLIIDQMLESREKQKRMEKLNMVIGTFFSTIGTPLLGQLAEADTSIDN
                     VKPRLVIGDDWRNEMFRDVQDCLNNYTCSVAITRINMESLRVYLVSHEDFLIRLVENP
                     MVFEHESFTDLILALNHLTEEMKARGDFSTLPAPDKSHLSNDIRRVYSLLILEWLKYM
                     EYLKIHYPYLFSLAMRKNPFDASASVIVKKDS"
BASE COUNT          929 a          770 c          893 g          884 t
ORIGIN      
        1 ggcctgaacc tacaacgtgt tgttcgatgt actggacaga cgggtgaaga tcgatcccat
       61 aacttgtcgc acttgcatcc agaagctgac caaaaaggag tgcaaggtaa agcggatcat
      121 tcacatagtt ccagcagagc aggtaacgca taaaggccca taccagcagg gttgcagtga
      181 tagccatgag aggaatgata gcgagtataa agagatcgac gtgggcatgg ttgatacccc
      241 atgcaagcaa gacaagcgaa acgacaatcg tggatactat gccggcaaac atgtaaaatg
      301 tgaggacgtt ctttgtaagt ccctgcaggg taatagatcg tgagagaaac agcatcgcaa
      361 ttgtaaagaa aaagagtacg aagtagataa gcggggtcac aagcaaaaac tggaagtcac
      421 ttttgatcat cccggtatcc tgcacgaccc gcaaaacacc gccgagaacg acatagggaa
      481 gtgtggcgag gataaaattg gcatcaattt taaatccgct gtctgagaga tatgtggatt
      541 gcgacatcca gcggtacaat aaatatactc cgatgaccag gattaccgcg tatgtaaggg
      601 tgtcgacaat attataggat tgcccaagcc tgatgggatc gatgtaatat ttgtatatga
      661 aatcactaat catccatgat cagaatgagc gcagatgcaa taaaattact caaaccaaaa
      721 atttttgaca agtccaaatg gatgcagctg ccacgggatg tcgttatagg tcatgatgtg
      781 ttcgggcaga tcccatcggt ctgtgatgat ctcaaactcg gttcttccgc gctcctgata
      841 tccggaaagg gtaccatggg tcttgccggg aacaaggtcc tggatattct caaaaaagac
      901 tataccgtaa agccatttat tgcagaagag atcagcattc ccatcattaa aaaggcacaa
      961 aaagccgcgg caggaatgga ctttgttctc ggtatcggtg gcgggcgtgt gatcgatacg
     1021 gcaaagattg tttcatacaa tcttgacctt cagtttattt ccattcccac agcggcatca
     1081 catgatggga ttgcttcgtc ccgtgcttcg gtgccgactg tggaggggca tgcgtcactg
     1141 gatgcccagc cgcccattgc tattgttgct gacacgggta tcattgcctc cgccccgcac
     1201 cggctccttg ctgccgggtg tgcggatgtg atctccaatt atacagcgat actggactgg
     1261 gagctcgccc accgggtgaa aggagaaccg atgagtgagt acgccatagc gctctcgaaa
     1321 atgactgctg agatcctggt aaaaaatgcc cacctgataa aaccgcacca ggagcagtct
     1381 gcgtggttcg tgacaaaagc gctcgtatcc agtggcgttg cgatgagtat cgcaggctcc
     1441 tcccgcccgg caagcggagg tgaacataag ttctcccatg cgcttgatag gcttgcaccg
     1501 ggcaaggcgc tgcatggcga gagttgtggt atagggacga tcatttccat gtacctgcac
     1561 ggtggagact ggagaggtat ccgggcatca ctgaaatgta tcggtgcccc ggtaacccct
     1621 gctgatgtgg gtattgatga tggaattgcc gtggaggctc ttctcatggc aaaggctatc
     1681 cgcccggagc gcttcacaat actcgatatg ggattgacgc gcgaatcagc cgagaatctg
     1741 atacagatgc tgtacaggga atgaggagtg gtcatatggc agaggcaaaa acaaaagtga
     1801 cgctggtcgg tacggttctg gctaaacagg gagttgattt catttacgaa ggtgaagttg
     1861 ccggctgcga tacctgtaaa gtaaaaaaag cgtgtaataa tcttcagaaa ggccggaaat
     1921 accgtattgt ctcagtgcgg acaactcatc atgaatgtat ggtccatttc aatggtgcaa
     1981 cggctgtaga agttatcgat gcaccggtga cagcgctcat taacgctgat atggctatca
     2041 ttaattcaaa gatcaagctc gatctatcct gcaacaggaa tgattgccgg agttttccgc
     2101 tgtgccaccc ggatggcgtt gtggaaggcg agaaatatat cgtttttacg atactgggta
     2161 atgcacctga tatctgcgag aaggggcggg cattgaagct tgtcgatatt aagccggcat
     2221 aggaatctct tattaaaaat ggaatgaggc accggggagg gcaaacaatg gctttcattt
     2281 ttcagcggaa aaatatcccc tcatttcaag cattcagaca aggcagccaa atggctgcat
     2341 gcacctcccg ggctggtaag gcatcgattc tccagtgagg atgctaaaag gagcgtgttt
     2401 tggtctgttt cctgggtaca tgtggtatct gatctgtgtt tttccggtcc ggcattcttc
     2461 cctcaattga actttcatag cggatttttt tatcatgaca aacaaccgat tgacttgagt
     2521 atgaaagagg atacacggtg gaaactcgtt gcagggattg ggcttgttac attatccctt
     2581 gcattgttta ccgcccatta cctgattttt caggatgcgc atcacctgtt catttttttt
     2641 atcggtgaca tcgccttcat cccgattgag gtgttgattg tcaccctcat cattgaccag
     2701 atgcttgaat ctcgtgagaa gcaaaaacgg atggagaagc tgaacatggt aatcgggact
     2761 ttcttttcca ctatcgggac tccgcttctc gggcaacttg ctgaagctga tacgtctatc
     2821 gacaacgtaa aaccacgcct tgttattgga gatgactggc gtaatgagat gttccgcgat
     2881 gtgcaggatt gcctgaataa ctatacgtgc agtgtcgcca ttacccgcat caatatggaa
     2941 tcccttcgtg tatatcttgt cagtcatgag gattttttaa tcaggcttgt cgaaaacccg
     3001 atggtctttg aacacgaatc gttcactgat ctcatccttg cacttaacca tctgaccgaa
     3061 gagatgaaag cacggggtga tttctccaca ttgcctgccc ccgacaagag ccacctctcc
     3121 aatgatatca ggcgtgttta ttccctgctg atccttgaat ggctcaaata tatggaatat
     3181 ctcaagatcc attacccgta cctcttcagc cttgcgatgc gtaaaaatcc gtttgatgcg
     3241 tctgcatcag ttatcgtgaa gaaggactca taggagccat cttttttttg agcctcatca
     3301 gcttgaaata ttgggcaggt gaattaccgg gcggaacctg gttttctcgt tttcggctct
     3361 catcgggcag tatacaggta agtaaagggg taattaaggg gtataacgga aatgacaatt
     3421 atagtttacg ggaattgttc tgcacaggac cagagctgtt ctgcacagtg caggat
//
LOCUS       DASRLV010000183         6841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_797353,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000183 DASRLV010000000
VERSION     DASRLV010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797353"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VFC43_07090"
     CDS             complement(<1..355)
                     /locus_tag="VFC43_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 12 protein"
                     /protein_id="HZK30978.1"
                     /translation="MFIGIDHGTTAMRFAGESGLFKVTREAAKGFAIQDLSQLCPIDE
                     IEGIALTYSMGDNISSITSIRKVKNRGLISQDGAGQHIGGGTKVFDEVAKSGIPAVLI
                     PGLHRGSPTDPRFKVY"
     gene            395..1339
                     /locus_tag="VFC43_07095"
     CDS             395..1339
                     /locus_tag="VFC43_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HZK30979.1"
                     /translation="MFDVVTGGAGFIGSHLVDTLVAQGNEVLVIDSLCAGKKETIARH
                     IDSCSVRFIKKDLLDDGWQEAVGGADRLFHLAADPDVRQSAVNPDPTMQNNIMATYRV
                     LEAVRLHNIPELVFTSTSTVYGDATVIPTPEIYTPLEPISVYGASKLACEALISAYCH
                     SFGMKAWIFRFANIIGSRSGHGVITDFIKKLEKNPAELEILGDGKQTKSYLEVHECVA
                     AMLFAVGHARGTVNTFNIGSEDWIDVKNIAEIVTEEMHLPDTKFKFTGGERGWVGDVP
                     KMQLSIDKIKALRWKPQTGSRESVRIAVRAMLEERISL"
     gene            1382..2533
                     /locus_tag="VFC43_07100"
     CDS             1382..2533
                     /locus_tag="VFC43_07100"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HZK30980.1"
                     /translation="MKYIVILGDGMADEPLAQLGGKTPLEYAKTPNMDRMARDGACGM
                     LRTIPEGFEAGSDIANMSILGYAPELYYTGRGPLEALSMGIDLDAHDVAYRCNLVTVD
                     NGIMTDFSAGHISSPEGAELFSLLGQRIPGVMFRPGVSYRNLIVLHDGKGAISTPPHD
                     IIGENIGNYLPKGPDAGLLLRCMEKSKEIFFEHPVNYARQKLGKPLATQIWPWSGGHK
                     PAFPSFETKYHKKGGIISAVDLLKGIARCAGMEVITVPGATGYLDTDYDAKGRYALEA
                     IKYLDFLYLHIEAPDEAGHLGNVEEKIRAIESVDSVIGMILEEFDSVLAVLPDHPTPI
                     RVKTHTRDPVPFVIMGKGKDATEQYSEQAARSGGFGMKNAVDFLDFLFS"
     gene            complement(2740..4722)
                     /locus_tag="VFC43_07105"
     CDS             complement(2740..4722)
                     /locus_tag="VFC43_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HZK30981.1"
                     /translation="MIIPQDNRPDPDTLLAHVQGEERQKHRGKLKIFLGYIAGVGKTY
                     EMLEAAHLRKNEGIDVRIGYIETHGRTETEALVEGISLIPRKMIEYRNVTIPEMDLDA
                     VLAARPQLVLVDELAHTNAPGSRHVKRYQDVEEILDEGIDVYTTLNVQHVESLNDVIA
                     QITGVIVRETIPDRVIDEATEIELVDLAPPELLQRLREGKVYVPDMAARAIEQFFNEG
                     NLFALREIALRRAAERVDDQMLAWMQTRAIPGPWAAGESLLICVGPSPFSERLIRTAR
                     RQADRMTAQWSAIYVETPSHHRLSKEAKEQVSRMLHLAENLGATTATVYGINVANAVI
                     EYARRHNITRIIIGKTLRPRWQEFVFGSVVDQLIHNSGTIDVYVISSEEKTLQKPVPM
                     EPLLPTTLPKDYLASLVLVAMITAIGWLIRSFISPVNLAMLFMLGVVFIAWRRGLRPA
                     IFTAVIGVVAFDFFFIPPYLSFRISDTEYLITFAGMIIVGALISLLVARAHEHADAAQ
                     IREKETGTLYALSQDLAVAMNVNSIVAAVAKHINGIFQWECVFLLPEGNELVMREASP
                     GLRLDADDIAVATWAYQHGTVAGYDTDTLHGSRLRYIPLKSTQGVLGIMGVKPAEPEG
                     VITHEQDRILMAFANQTALALERVNLVRGLPRIRER"
     gene            complement(4732..6834)
                     /gene="kdpB"
                     /locus_tag="VFC43_07110"
     CDS             complement(4732..6834)
                     /gene="kdpB"
                     /locus_tag="VFC43_07110"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HZK30982.1"
                     /translation="MSDQSSAKHASEKQYALYKRAVIDAFLKLDPRTLYHNVVMFTVE
                     IGSVITTLLFLQSLSGKGEASPGFIGAITAWLWFTVLFANFAEALAEGRGKAQAEALR
                     KMRRDTEAKKLYLGYEVMKGKEPAPTDFTMVSSLTLLKSDLYFVKAGDTIPVDGEIVE
                     GVASVNESAITGESAPVIRESGGDRSAVTGGTVILSDWLIIRAIANPGEGFLDHMISL
                     IEGAKRQKTPNEIALNILLVGLTAIFVVVCASLWAFSIYSVKAAGSGVPVTITVLIAL
                     FVCLAPTTIGGLLSAIGIAGMDRLIRRNVITTSGRAIEAAGDIDVLLLDKTGTITLGN
                     RQAVELKPVDGTDILELAETAQLASLADETPEGRSIVVLAKEMYGLRGRTVGASESAP
                     EMQFIPFSSHTRMSGVDQGDVHIRKGAADAIFAYVRENGNGISDELKQEVDAIARAGG
                     TPLVVTKNGKALGVINLKDIVKGGIKERFAQLRRMGIKTIMITGDNRLTAATIAAEAG
                     VDDFLAEATPESKLKMIREYQAGGRMVAMTGDGTNDAPALAQADVAVAMNTGTQPARE
                     AANMIDLESNPTKLIEIVEIGKQLLMTRGALTTFSIANDLAKYFFIIPAAFVSTFPAL
                     GVLNILGLHNAILSAVIFNALIIVALIPLALHGVAYHPMTAEESLRKNILIYGIGGVI
                     APFIGIKIIDIILVLIGM"
BASE COUNT         1599 a         1773 c         1806 g         1663 t
ORIGIN      
        1 aatatacttt gaaacgggga tcagtcggtg atccgcggtg aaggcccggg atcaaaaccg
       61 cagggatccc gctttttgca acctcatcaa atacttttgt tccaccgccg atatgctgtc
      121 cggcgccatc ctggctgata aggccccggt tttttacctt tctgatactg gtgattgagg
      181 aaatattgtc acccatcgaa tacgtgagcg caatcccttc aatttcatca atcggacata
      241 attgcgagag atcctgtatt gcgaaccctt ttgccgcttc gcgtgtgacc ttgaataatc
      301 cggactctcc ggcaaaacgc attgcggttg tgccgtgatc gatcccgata aacataacag
      361 taatcctata gcatggatca catattaggt attgatgttt gatgtcgtaa cgggtggtgc
      421 cgggttcatc ggttctcacc ttgttgatac tctcgttgca cagggcaacg aggttttggt
      481 aattgactcg ctatgcgccg ggaagaagga aacgattgcc cggcatatcg attcctgcag
      541 cgtacggttt atcaaaaaag atctcctgga cgatggatgg caggaggctg ttgggggagc
      601 ggacaggctc tttcatctcg ctgcagatcc tgatgtccgc cagagtgctg tcaaccccga
      661 tccgaccatg cagaacaata ttatggctac ctacagggtg ttggaagctg tgcgcctgca
      721 caatattccc gaactcgtat ttacgtccac atccacggtc tatggagacg caaccgtcat
      781 ccctacaccg gagatctata cccctcttga gccaatttcc gtatacggtg ccagcaagct
      841 tgcctgtgaa gcgcttatct ctgcctactg tcactcgttt ggtatgaagg catggatatt
      901 ccggtttgcc aatatcatag gttcgcgtag tggccacggt gtcataaccg attttattaa
      961 aaaactggaa aaaaatcccg ccgagcttga gattcttggg gacggaaaac agaccaaatc
     1021 atacctggag gtgcacgaat gcgtggcagc gatgctcttt gctgtcgggc atgcccgggg
     1081 gacggtcaac accttcaata taggttcaga agactggatt gatgtaaaaa acatcgcgga
     1141 aattgtcaca gaagagatgc acctgcctga tacgaagttt aagttcaccg gaggtgaaag
     1201 gggctgggtc ggtgatgtgc caaaaatgca gctttccatt gataaaatca aagcccttcg
     1261 ctggaaaccc cagaccggtt cacgcgagag cgtgagaatt gctgtccggg caatgcttga
     1321 agaacgaata tctctttaac gatggcaatc aggcgataaa ttataaaaaa ccgtgattct
     1381 catgaaatat atcgttattc tcggtgatgg catggcggat gaaccgcttg cgcagctcgg
     1441 cggaaaaaca cctcttgaat acgcaaaaac accgaatatg gaccgaatgg cacgagatgg
     1501 tgcatgcggg atgctccgga caattcctga aggttttgaa gcgggaagtg atattgccaa
     1561 catgtcaatc ctcggttacg ctcctgagtt gtattatacc ggcaggggac cgctcgaagc
     1621 tttgagcatg ggaattgacc tcgatgccca tgatgttgca taccgctgta atctggtaac
     1681 cgtggataac ggtattatga ccgatttctc agccgggcat atttccagtc ctgaaggcgc
     1741 tgaactcttc tccttactcg gacagaggat tccgggagtg atgttccgac cgggagtcag
     1801 ttatcgaaac ctgattgttc tccacgatgg caagggggcc atttctaccc cgcctcatga
     1861 tattattggg gaaaatattg gtaattatct tccaaaagga ccggatgccg gtctgcttct
     1921 gcggtgcatg gagaaaagta aggagatatt ttttgaacac ccggtgaatt atgcacgcca
     1981 gaaactggga aaacccctgg cgacccagat ctggccctgg agtggcgggc acaaacccgc
     2041 atttccctca tttgagacca agtaccacaa gaaaggcggg ataatatctg cagtggacct
     2101 cttaaaaggg atcgcacggt gtgccggtat ggaagtgata accgttccgg gtgctaccgg
     2161 ctaccttgat acagattatg acgccaaggg acgctatgct ctcgaagcaa tcaaatacct
     2221 tgacttcctg tatctccaca ttgaagcgcc ggatgaagcg ggacatctgg gcaatgttga
     2281 agagaagatc cgcgcaattg agagcgttga tagcgttatc ggaatgattc ttgaagagtt
     2341 tgatagtgtc cttgcggtgc ttcccgatca tccaacccct atcagggtta agacccatac
     2401 gagggatccg gtcccgtttg ttattatggg taaaggaaaa gatgcgacgg aacaatactc
     2461 tgaacaggca gcccggtccg ggggattcgg gatgaaaaat gcggtagact tcctggattt
     2521 cctgttttca tgagatgtgc ggcatcagac ccctgcttca tcatctgttt tcagtggggg
     2581 taactttcat catttgccac aaaatattaa tatattgttg atttcacaat tatataaact
     2641 tattgtataa ttatcggatt ctgaagacct ttcaccagcc tctgcagtgg tccggcccaa
     2701 gacatgcaga taaatttact atccttcacc cgaatcactt catctctcac gtatcctggg
     2761 aagtcccctc acaagattca cccgctccag cgccagagcg gtctggttgg caaaggccat
     2821 gagtattcta tcctgttcat gggtgatgac accttcaggt tctgccggtt tcactcccat
     2881 tatcccgagc acgccttgcg tactcttaag gggaatgtac ctgagccgtg atccatggag
     2941 cgtatcagtg tcgtaaccgg caacggtccc gtgctggtaa gcccatgtgg caactgcaat
     3001 atcatcagca tcaagccgca ggccgggact ggcctcacgc ataaccaact catttccttc
     3061 aggcagaagg aatacacact cccactgaaa aatcccgttg atatgctttg cgactgcagc
     3121 gacaatcgaa ttgacattca ttgctaccgc aagatcttgc gaaagtgcgt acagggttcc
     3181 ggtctccttc tcccggatct gggctgcatc tgcatgttca tgtgcacggg cgacgagcag
     3241 gctgattaga gcgcccacga tgatcatacc ggcaaatgtg atgagatact cggtatccga
     3301 tatgcgaaaa ctcaggtagg gagggatgaa gaagaaatca aacgccacaa ccccaatgac
     3361 tgcagtgaag atggccggtc gtaatcctct cctccatgca atgaaaacaa cgccaagcat
     3421 aaacagcatg gcaagattta ccggggaaat gaatgaacgg atcagccagc cgatggcagt
     3481 gatcatggca acaagaacca ggcttgccaa ataatccttc ggtaatgttg tcgggagcag
     3541 gggttccatg ggtacgggtt tctggagtgt tttttcttca ctgctgatga catacacatc
     3601 tattgtaccg ctgttgtgga tgagctggtc caccaccgat ccaaagacaa attcctgcca
     3661 tcggggtcgc agggtttttc cgatgatgat acgcgtaata ttgtgcctgc gtgcatactc
     3721 tatgacagca tttgcaacat taatcccata tacggtcgca gttgtggcac cgagattttc
     3781 tgccagatgg agcatgcggg acacctgttc ttttgcctcc tttgagagac gatgatgcga
     3841 tggcgtctca acatagatcg cgctccattg ggcagtcatc cggtctgcct ggcgccgggc
     3901 agtacggatt aacctttcgg aaaatgggct gggcccgaca cagatcagca ggctctcccc
     3961 tgctgcccac ggccccggga tcgcacgggt ctgcatccat gcaagcatct ggtcatcgac
     4021 ccgctcggct gcccggcgga gagcaatttc ccggagtgca aacaggttac cttcattgaa
     4081 gaactgctca attgcccgtg ctgccatatc aggtacatac accttgcctt ccctgagtcg
     4141 ctggaggagt tccggtggtg caaggtcaac aagctcaatc tcggtcgctt catcgataac
     4201 ccgatcggga atggtttcgc ggacgatcac cccggtgatt tgggcgatga catcgttgag
     4261 gctctcgaca tgctggacgt ttaatgtggt gtatacgtct ataccttcgt caaggatctc
     4321 ctcgacgtcc tggtacctct ttacgtggcg cgaacccggc gcattggtat gggcgagttc
     4381 atcaacaagg acgagttgag gtcgggctgc aaggactgca tcgaggtcca tttcaggaat
     4441 ggtcacattc cggtactcga tcatctttct cgggatgagc gatattcctt caacgagtgc
     4501 ttctgtttcg gtcctgccat gcgtctcgat atacccgatt cttacatcta ttccctcatt
     4561 cttccggaga tgtgccgctt ccagcatctc atacgtcttc cccacaccgg caatgtaacc
     4621 aaggaaaatt tttagtttgc cccggtgctt ctgccgctct tcaccctgta catgggcaag
     4681 aagggtatcg gggtccgggc gattgtcctg aggaataatc atagaaatcg tttacatacc
     4741 tatgagcacg aggatgatat cgatgatctt gataccgatg aacggggcga tgaccccgcc
     4801 gatcccgtag atgaggatat tcttccgcaa tgattcctcc gcggtcatcg gatggtacgc
     4861 aaccccgtgc agggcgaggg gaatgagggc gacaatgatg agggcattga agatgacagc
     4921 tgagagaatc gcgttgtgga gaccgaggat gttcagaacc ccaagtgccg ggaacgttga
     4981 cacaaacgca gcgggtatga tgaagaaata ttttgcaagg tcgtttgcga tggagaaggt
     5041 agtcagtgct ccccgggtca tcaggagctg ctttccgatc tcgactatct cgatgagttt
     5101 tgtggggttg ctctcaagat cgatcatgtt cgccgcctca cgggccggtt gggtaccggt
     5161 attcatcgca acggctacgt ccgcctgggc gagagcgggg gcatcgttgg tgccgtcacc
     5221 ggtcatggct accatccgcc ctcccgcctg gtactctcgg atcattttga gtttgctctc
     5281 cggagtcgct tccgcgagga aatcgtccac tccggcttcg gcagcgatgg tcgcagcagt
     5341 gagacggtta tcaccggtga tcatgattgt cttgatcccc atacggcgga gctgtgcaaa
     5401 ccgctcctta atcccgcctt ttacaatgtc ttttaaattg atgacgccga gagccttccc
     5461 gttctttgtt acgacaagtg gggttccccc ggcacgggcg atagcatcaa cttcctgttt
     5521 cagctcgtct gaaatgccat tgccattttc cctgacatac gcaaaaatag cgtctgcagc
     5581 gcctttccgg atatggacat ctccctgatc gacaccgctc atccgggtat ggctggagaa
     5641 cggtatgaac tgcatctccg gggctgactc ggatgcaccc accgttctcc cgcgaagccc
     5701 gtacatctct tttgcgagaa cgacgatgct gcgcccttcc ggtgtctcat ccgcaagcga
     5761 agctagctgt gcagtctcgg caagttccag aatgtcggtc ccatcgacgg gtttcagttc
     5821 gacagcctgc cggttgccaa gcgtgatcgt ccccgtctta tcgagaagga ggacatcgat
     5881 atctcccgct gcttcaatag ccctgcccga ggtcgtgatc acgttgcgcc ggataaggcg
     5941 gtccataccc gcgatgccga tcgcgctgag cagacccccg atagtggtcg gagcgagaca
     6001 gacgaagagt gcgataagca ctgtgattgt gaccgggacc ccggagcccg cagccttcac
     6061 gctgtagatc gagaacgccc aaagcgaggc gcagactaca acaaagattg cagtcagccc
     6121 tacgagaagg atgtttaggg cgatctcgtt cggggtcttc tggcgtttcg caccttcaat
     6181 aaggctgatc atatgatcga ggaagccctc gccgggattg gcaatcgccc ggataatgag
     6241 ccagtccgac agaataaccg ttccaccggt gacagcgctc cggtcgcctc cggattcacg
     6301 gatcaccggt gcactctctc cggtgatcgc gctttcgttg accgaagcga ctccttcgac
     6361 gatttccccg tccaccggga tagtatcacc ggctttaaca aagtagaggt ctgacttgag
     6421 cagcgttaag gaagagacca tggtaaaatc agttggagcg ggttcctttc ctttcatgac
     6481 ctcgtacccg agatagagtt tcttcgcctc ggtgtcccgc cgcattttcc gcagggcttc
     6541 ggcctgcgct tttcccctgc cttcagcaag ggcctctgcg aagttggcaa agaggaccgt
     6601 gaaccagagc caggcagtga tcgctccgat gaaacccggt gaagcctctc ctttccccga
     6661 gagggactgc aggaagagaa gggtggttat cacgctgccg atctcgacgg tgaacatgac
     6721 cacgttgtgg tacagggtcc gcggatcgag ttttaggaag gcgtcgatga ccgcacgctt
     6781 gtaaagagcg tactgtttct ccgatgcatg ttttgcgctg ctctgatcag acatggatga
     6841 g
//
LOCUS       DASRLV010000184         5792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_797758,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000184 DASRLV010000000
VERSION     DASRLV010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797758"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(378..1466)
                     /locus_tag="VFC43_07115"
                     /pseudo
     CDS             complement(378..1466)
                     /locus_tag="VFC43_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107301.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
     gene            complement(1459..3261)
                     /locus_tag="VFC43_07120"
     CDS             complement(1459..3261)
                     /locus_tag="VFC43_07120"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HZK30983.1"
                     /translation="MVLADELAKQQRSISVAEFFEKNKHLLGFDSPTRGVITTIKEAV
                     DNALDACEEAQVLPDIYISIKKISNEIFRIIVEDNGPGIVPAQVPFVFGKLLYGSRFH
                     QIRQTRGQQGIGISAAVLYAQLTSGVPTVIISRTGAKEPAYRCEIQIKIETNEPDIIS
                     QQAFEWDRIHGTRVQIEFKSTMSAKKKLLEYLRYTSIVNPHARFRVELDDEAFTFDRV
                     SHEIIACPIAIQPHPHGIEFGQLKRMAAARNENLVDFLVQGFSRVGKKTAQEMCDQSG
                     LKQSVKVKNLSADQLKSLLGAMQSVAVPAPPTTQCLSPIGDELIRRGLDKEFQMDFVA
                     ARTRPSSVFSGHPFMVEAAIGYGGKLPVEGTAVILRFANRVPLMYQQGACAITSCIAS
                     VNWKSYNITQQGLPMGPVLILVHVASTNVPFTSESKDAIASIPEIEKEIILALQDLGR
                     DLKLFVSRRDKSKVAEDRARAVCAIIPEIAAKVSEIVEKPLIDTSPIEGRLLKKLIAK
                     KWNREGKVTIELNNYTGHDGEISIYDISADTASDAVPRADFVSEMDGQFTKVWKTVIP
                     PQTVWQVTYTGKGGGLLDIRGIEDNKKMVVDLDV"
     gene            complement(3248..4480)
                     /gene="hisS"
                     /locus_tag="VFC43_07125"
     CDS             complement(3248..4480)
                     /gene="hisS"
                     /locus_tag="VFC43_07125"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HZK30984.1"
                     /translation="MLQKPRGTRDFLPDEMEARRTIEWRMREIARRWGYREVCTPEFE
                     DLELFTMRSGEGIIEEMYVFEDKGGRKLALRPEITAAVIRMYINEAKVAPKPLRWCYF
                     ADCFRYERPQKGRYRQFWQFGVELIGADSAIADAETIMLAAEMLNATGVSYDLKVGHL
                     SFMKNLLKDLEPQVQRRVMAHLDKKDFDGLRGTLESMGKADLGTSLVSLVECRELDEA
                     FEIAGSIPEKDRINKTKEFLDTAGVKYSLNFGIARGLDYYTGMVFEGFAQNLGAENQI
                     LGGGAYRLAHLFGGDDVGSCGFAIGFDRVMVSLGEIPKKKDLVVAIVCTDEGRLHALR
                     VAGVFRNSGIRTEMDLMERGLGAKLAHASKTADYAIVIGQRESVSGTVTLKNLQSGEQ
                     KTLDPQSAVAEVASHGAR"
     gene            complement(4518..4832)
                     /locus_tag="VFC43_07130"
     CDS             complement(4518..4832)
                     /locus_tag="VFC43_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK30985.1"
                     /translation="MTYLTYVAIFGALAIVFLWLRDVRIFYRTGLEGYRKASYWGVGY
                     SALATLGVFFTVSMLELLGLGVILGALYLQGRIEREKVWGSEGTLDRFLGSVPIRRAN
                     KK"
     gene            4884..5660
                     /gene="larB"
                     /locus_tag="VFC43_07135"
     CDS             4884..5660
                     /gene="larB"
                     /locus_tag="VFC43_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HZK30986.1"
                     /translation="MVPNPTLQDLLSRYKNGEITLESAAEEIEGLRLEHIGDFACIDL
                     GRSMRCGMPEVVLAEGKEPEHLARIALRHAEATGRCVITRVLPEQVAFIRNFTAGTEI
                     NSQYHETARILVLSHGTSPKLTGGIVAVITAGTSDIRVAEEAKIIAEEMGCEVRTAYD
                     VGAAGIHRLFPALRPMLSAHVFIVCAGREGTLPAVVAGLVDKPVIGVPVSTGYGYMGE
                     GRAALASMLQSCSVIAVVNIDAGFTAGAFAARIANGGVKQ"
     gene            5657..>5792
                     /locus_tag="VFC43_07140"
     CDS             5657..>5792
                     /locus_tag="VFC43_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HZK30987.1"
                     /translation="MRRGFYIGRFQPYHNGHQSVLEHIADDVDEIVIGIGSAQLSHLT
                     D"
BASE COUNT         1335 a         1532 c         1437 g         1488 t
ORIGIN      
        1 gatatactta tgggaactgg cggacagcga ttgggtactt acttcggcag gtctggagaa
       61 aaaacagttc tcgttacgca cagttggaca ggtgctcgtc ctgtcagcac cccaaaaatt
      121 ccaaatcatt ctcgaaccgt ttttcaggct cggacacatc ctgaaatacg gatatttgaa
      181 attatctaag cacttgcgaa tataaccata tactcaattg aaaatttcag atatggtaat
      241 aaaatctgtt gaaaaaagtt acaaaataaa aaataactat tccttttttg ttatccacgg
      301 acttaccggc gacagatatc gaggaaatat atcccggata attacccggc ccggtatttc
      361 cgtatcatca tagtatgtca gataatatcc atctcactca gccgtgccgg cagatacgtc
      421 ttggtcacga aatcaaggcc ccgtgcagca aacgcctgtt gttctgactt cagtcccagt
      481 tcaatctgga ggttgatctg tttcttccag tattcggttg caaaccttgg atcggtcagt
      541 tctgcattga gggcttcaac atccttgtct gtcagtttat aggaaggcag gttgtaatcc
      601 cgaatatcgc tgggctgcag gcctaaaaac tgtgcccggg gagttgccag cagctctgac
      661 atatgggcac ttttaattgc cccgtaggct accgaagcga agatacgata ggaccatggg
      721 tcaccgtcag tgaatacgag aaccgggagg ttccagttgg agctgatctt gttcagcata
      781 cgccgcgtgg aacgggcagg ttgtcctttt aaatgaacga ggatcgcatg gtagtcttcg
      841 tcaaatccgt tctcaataag ccggtcatac ataccaccgg tctcaagagc aatgacaaac
      901 tttgcatcgt gctccacaaa ctcaatgttt tcgacattgt tgggtatcgt gtaccctgcc
      961 tgtcatacgt catcctggca atggatggca cgcatccccc tgcgggtctc ttcccggatc
     1021 ttcatcgggc catagatgga tgcaccgttc tcttcggggt ggagatgaaa cccctcccgg
     1081 gggacttcgg agataatctc gaggtcttcg ataagaaaat tgctttcctc ctgagccccg
     1141 aactttgccc gtttccatcc ctcagagata tagtacattt cccttaatgt tgatgagcgg
     1201 ttttcacgga gctgctgctt gataaacccg atcacgtatg ccatcttcag gaggtgggtt
     1261 gcactctttg ccgaagcagc cgtgcgcatg ctttccttgt cgccatattt ccagacttcg
     1321 ctggtatcat cgaactcgat attgtatttt gtgcgggttg gcaacgagat ggaagggatc
     1381 tctcctgctt tcatctggtc ataccacgaa gtcgcgatcc tgagcagcgc tatcatcgac
     1441 tttttttcca gttcttcttt agacatcgag gtcgaccacc atttttttat tgtcttcgat
     1501 accccggata tccagcaacc ccccgccctt tccggtatag gtgacctgcc acaccgtctg
     1561 gggggggatc accgtcttcc agactttcgt gaactgtccg tccatctcac tcacaaaatc
     1621 cgcccgggga accgcatcgg aagccgtgtc ggcagatata tcgtaaatag aaatctcccc
     1681 gtcatggccg gtataattgt tcagctcgat ggtcaccttc ccttcacggt tccatttttt
     1741 ggcgatgagt ttttttaaga gcctgccttc aataggcgag gtgtcaatga ggggtttttc
     1801 cacaatttca ctcactttag ctgcgatttc cgggattatt gcacagacgg cccgtgcccg
     1861 gtcctctgca actttgcttt tatccctgcg ggatacaaag agtttgagat cccgcccgag
     1921 gtcctgtaat gcgagtatga tctctttttc aatctcggga atacttgcga tcgcgtcctt
     1981 gctctcgctc gtgaacggga catttgtcga tgcaacatgg acaaggatga gaaccgggcc
     2041 catcggaagc ccctgctggg tgatattgta ggatttccag ttgacactgg caatgcagga
     2101 ggtaatcgca caggcgccct gctggtacat gagcgggaca cggttggcaa atcggaggat
     2161 gacggcagtt ccctcaaccg gcagtttccc gccataccct attgctgctt ctaccatgaa
     2221 gggatggccg gagaaaacag aacttggtcg tgtgcgtgcc gcaacaaaat ccatctggaa
     2281 ctccttatca agcccccggc ggatcagttc atcgcctatc ggggagagac actgggtcgt
     2341 gggcggtgca ggaacagcaa ccgactgcat cgcgccgagg agcgatttga gttgatccgc
     2401 ggacagattt tttactttta cggattgttt aagaccggat tgatcgcaca tttcctgagc
     2461 agtcttctta ccaacccggg aaaagccctg tacgagaaaa tccaccaggt tctcatttct
     2521 tgctgctgcc attcttttga gctgaccgaa ttcaatgccg tgcgggtggg gctggattgc
     2581 gatagggcag gcaataatct catgactcac ccggtcaaag gtaaatgcct cgtcatcgag
     2641 ttcgaccctg aaccgtgcgt ggggatttac gattgatgtg tacctgaggt attcgagcag
     2701 cttcttcttt gccgacatcg tgctcttgaa ctcgatctgg accctggtcc catggatgcg
     2761 gtcccattcg aaggcttgtt gcgagataat gtcaggttcg ttggtctcaa tctttatctg
     2821 gatctcgcac cggtatgcag gttcctttgc acctgtccgt gagatgataa ccgtcggaac
     2881 tccgctggtg agctgggcat agagtaccgc tgctgagata ccaataccct gctgtccgcg
     2941 ggtctgccgt atctggtgaa accgtgagcc atagaggagt tttccgaaca caaacggtac
     3001 ctgggcaggg acgataccgg gaccattgtc ctcgacaata atccggaaaa tttcattgga
     3061 aatttttttt atactgatat agatatcagg aaggacttgg gcctcttcac acgcatccag
     3121 tgcattgtca actgcttctt ttatggtggt gattacgccg cgggttggtg aatcaaaacc
     3181 gagaaggtgt ttgttcttct caaaaaattc tgcgacgctg atgctgcgct gctgtttggc
     3241 aagctcatca gcgagcacca tgtgatgcca cctcagccac cgctgattgc ggatccaggg
     3301 tcttctgctc tccgctctgc aggttcttga gcgtcacggt tccggagact gattcgcgtt
     3361 gtcctatcac aatagcataa tccgctgttt tggatgcatg agcgagcttt gcgcccagtc
     3421 cacgctccat aaggtccatc tcagtccgga tacctgaatt ccggaaaact ccggcaaccc
     3481 tgagcgcatg gagccggccc tcgtccgtgc agactattgc gacaacaagg tcctttttct
     3541 tcggtatttc gccaagcgac accatgaccc ggtcaaaccc tattgcaaac ccgcaggacc
     3601 ccacatcatc cccgccaaaa agatgagcca ggcgatatgc accgccaccg aggatctggt
     3661 tctctgctcc caggttctgg gcaaaccctt caaagaccat accggtatag tagtccagtc
     3721 cgcgggcgat accaaaatta agggaatact ttacacctgc agtatccagg aactctttgg
     3781 tcttgtttat gcgatctttt tcagggatcg aacctgcaat ctcaaatgcc tcatcaagtt
     3841 cacgacattc aaccagggaa acaagggaag ttcccagatc tgccttgccc atggactcaa
     3901 gcgtaccccg caagccgtca aagtcctttt tatcaaggtg ggccatcacc ctgcgctgca
     3961 cctgcggttc aaggtctttt aacaggtttt tcatgaacga gaggtgtccg actttcaggt
     4021 catagctgac acccgtggcg ttgagcattt cagccgcgag catgatagtt tcagcatcgg
     4081 caatggcact gtcagccccg ataagctcca ccccgaattg ccagaactgg cggtaacgcc
     4141 ctttctgggg gcgctcgtac cggaaacagt cggcgaaata gcaccagcgc aggggttttg
     4201 gtgcaacttt tgcttcgttg atgtacatcc ggataactgc cgcagtaatc tccggccgaa
     4261 gggcaagctt tcgcccaccc ttatcctcaa aaacgtacat ctcctcgata atcccttcac
     4321 ccgagcgcat cgtgaagagt tccaggtcct caaattccgg cgtgcagact tcacggtacc
     4381 cccagcgccg cgctatctca cgcatgcgcc actcgattgt ccgtcgcgct tccatctcat
     4441 ccggtaaaaa atcccgtgtc cctctcggtt tttgaagcat aggaaaagtc ccttgttctg
     4501 tactgttggt gttagtgtta tttcttattc gccctgcgga tcgggacact tcccaaaaaa
     4561 cggtcgagtg ttccttcact tccccagact ttctcccgtt caattctgcc ctgcagatag
     4621 agggcaccaa ggatcactcc tagtcccagc agttccagca tagagacggt aaaaaagact
     4681 cccagggttg caagggcact atatcccact ccccaatatg acgccttacg atagccttca
     4741 agaccggtgc ggtaaaatat ccgcacatcc ctcagccaga gaaacacgat cgccagggca
     4801 ccgaaaattg caacataggt gagataagtc atcgcaataa actaacttat tatacgctgt
     4861 tccatatctg tattattacg atcatggtac cgaacccgac gttacaggat ttactgtcgc
     4921 gctacaagaa tggtgagatc accctcgaga gcgcagcaga agaaattgag gggctgcggc
     4981 ttgaacatat tggtgatttt gcctgtattg atcttgggag atccatgcgg tgtggtatgc
     5041 cggaagtggt gcttgccgaa ggtaaagagc cagaacacct ggcccggata gctcttcggc
     5101 atgcagaggc aacaggtcgt tgcgtaatca cgcgcgtcct ccctgaacag gttgccttta
     5161 tccggaattt caccgcaggt acggaaatca attcgcagta ccacgaaact gcacggattc
     5221 ttgtgttatc acacgggact tctccaaaac tcaccggggg gatcgtagct gtaattactg
     5281 ccggaacatc tgatatcagg gtagctgaag aagcaaaaat cattgctgaa gagatggggt
     5341 gtgaagtccg gactgcctat gatgtcgggg ctgccggcat ccaccggctc tttccggctc
     5401 tcagacccat gctctctgcc catgttttca ttgtgtgcgc cggcagggaa ggcacccttc
     5461 cggcagttgt cgcgggtctt gtcgacaagc ccgttattgg ggtcccggtg agcaccgggt
     5521 acggctacat gggtgaaggc agggcggcac tggcaagtat gctccagtcc tgttcggtta
     5581 tcgctgtagt aaatatcgat gccggtttca ctgccggggc atttgcggcc cgtattgcaa
     5641 acgggggagt caaacaatga gacgcggttt ttatatcggg cggtttcagc cgtaccataa
     5701 cgggcaccag tctgtgcttg aacatattgc agatgacgtg gatgagattg ttattggcat
     5761 aggaagtgcc cagctttcac accttacgga ca
//
LOCUS       DASRLV010000185         6938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1033468,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000185 DASRLV010000000
VERSION     DASRLV010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 6938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6938
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033468"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VFC43_07145"
     CDS             complement(<1..739)
                     /locus_tag="VFC43_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZK30988.1"
                     /translation="MTTIFVDQDLCTRCGICSTVCPMKIIDPAYESTLPRVPEAKATM
                     CIRCGHCEVSCPSQAVTLNFLADEKVSLAAGAGSLSPEDIGIYLKNRRSVRHYTPEPV
                     DKETITRILDVARYAASGGNGQPVQWLVVHDPKEVHLLAGPTIDWMKTLLHSNHPMSG
                     MVPSIVKAWEHGYDPVCRGAPHLVVAHIPEGNPVASIDAIIALTHFDITAPAYGAGTC
                     WAGFLSMAAAAYQPLQKALALPSGRTYA"
     gene            complement(917..1390)
                     /locus_tag="VFC43_07150"
     CDS             complement(917..1390)
                     /locus_tag="VFC43_07150"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HZK30989.1"
                     /translation="MRWYHTAGACLLIISCVVVAGCSLLFKNPDVSVTTVTPTSLSLS
                     AISLNVTLHVDNPNPIGIPFKKIAFDAWFHDGTNWNAITHAEQGAIEIKPGKTGITIP
                     VSVRYTDMCTAGWSIISRHELPLKISGTASPDFFGIAPELPFTYNTTIPLALPGI"
     gene            complement(1481..2143)
                     /locus_tag="VFC43_07155"
     CDS             complement(1481..2143)
                     /locus_tag="VFC43_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HZK30990.1"
                     /translation="MDIGKTVGDSFEYAQEGLVGRWMKWFLLLISCIIFPLFMGYTMR
                     IYRGVTPSPEPDDWGTMFIDGIKLIIVGIIYALPVIILEIVIIGSAGLALFMGPRQSV
                     HPGAVLGLIGAVLAGVLVLLVVALIIGLIATIAYVRFARTGSFGEAFNFSAIFATIGR
                     IGWITYIFALIVMMLIIGVIEVICFVIPILGIVLFLILLPFIGIFSARYITLLYDSAG
                     AD"
     gene            complement(2344..3105)
                     /locus_tag="VFC43_07160"
     CDS             complement(2344..3105)
                     /locus_tag="VFC43_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK30991.1"
                     /translation="MTDGLDWYGQIYAELNAAWAIAKKDIAIYYLKPNIIVSGALFPL
                     FMFLAFAAGNSAGPSVMIPGLIAITILFSASSIEPVSIPIERRMKTFDRLISAPISLH
                     AVVLGESLSGFLYSMGIAFVPLIAGLLIFSTPIVSIAPLVMGIILTSFCFATMGTLFA
                     AYPTESPGDIMSMLNLVRLPLIFISGVFIPIESIPSIGQGAAYFSPLTYGNDLIQSAY
                     HGHTHFNPLIDIAMLCVFILIFQVFANQLYRRWNE"
     gene            complement(3098..4054)
                     /locus_tag="VFC43_07165"
     CDS             complement(3098..4054)
                     /locus_tag="VFC43_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZK30992.1"
                     /translation="MYAIEVNHLSKHFGALIALNDISFSVTEGETFGFLGPNGAGKTT
                     TIRVLTGISAPTGGTAMVFGKDIVKETTAARKQMGIVHETSNIYDDLSAWQNMMFTAE
                     LYTIGKKEREQRGKELLELFGLWERRTDKVHGFSKGMKRRLTLAMGLINNPRLLFLDE
                     PTSGLDVQSNLIIREVIRDLNSRGVTVFLTTHNIEEANLTCDRVAIINRGRIAAIDSP
                     ERLKATIQSAQSVEVAFDRSAAGFGSDLRQLNEVSEVRKEGDKFRLFTQNPSEVIAAV
                     MEYAAVNNTRVLSINTLGPSLEDVFIKLTGLEVRTKGVSTVD"
     gene            complement(4214..5206)
                     /locus_tag="VFC43_07170"
     CDS             complement(4214..5206)
                     /locus_tag="VFC43_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZK30993.1"
                     /translation="MTQMDAEIKAELLALGKVNVEPSLLPRPSRSTAGPGTGLRSIFF
                     KSGGHRVRLEIDADSPLKMVQQDGEVVIIRDDKELVRGKIDPIVAHCPEQTYITISEQ
                     CMYDCKFCSVPKFQGRIKTLEQVLAIVEKVQKDGKLKTIAFTSGVATTPENEIDRVVE
                     YVQAVKKYNVPIGVAVYPTHDSSRRLKDAGVTEVKYNVETMDRKIFEQVVTGRKGHSL
                     DFILDSLRDAVKVFGKNRVSSNIIIGLGETDECVRKGVEYLSKMGVIAVLRPITISPY
                     RKGEISATRPGAERLLKLTKMTGDILKKYDLHVEVSQTMCLSCTGCDLTPGRDM"
     gene            complement(5330..6193)
                     /locus_tag="VFC43_07175"
     CDS             complement(5330..6193)
                     /locus_tag="VFC43_07175"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZK30994.1"
                     /translation="MILDARVRPPYKSLKDVLAPAPGARPSVKRSPLVSGYERPQSMV
                     QKSVVLFLQEMDEAGVDKGILVGRQAGHRIVSNDDIVELRDQYPGRFPLSIAGINGIG
                     VPAAVREVERTITDRGFNGIAVDPGWWVPPMYVDDPRIYPIYAKCAELGGVLYLTCSL
                     MQGPDISYAEPWRIQRVANDFPDLQIVVVHGAFPYTSEMLGVMIANAPLGNLWVLPDF
                     FQFIPGFPGGQDWVEAANHYLGDRILYASSYPVRPLKQSLEEFRRFSYKPGVIENLLG
                     KNAANLFGIKI"
     gene            complement(6372..6788)
                     /locus_tag="VFC43_07180"
     CDS             complement(6372..6788)
                     /locus_tag="VFC43_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK30995.1"
                     /translation="MVTEDSDPEDHPVVNPRRNGEKRVNSPIKKQPVRKKKAPGSCIP
                     RPKDLPQEVKCNYPGWQKFLKRYPMINSLQREDFPDHGVSFVYLAHIYCSKNSAEESS
                     LSQKEFEAFFKKIGYEYDYSGNFESRWFEGIGVKIN"
BASE COUNT         1678 a         1731 c         1786 g         1743 t
ORIGIN      
        1 aggcgtacgt gcgtcctgaa ggaagggcaa gggctttttg caggggctgg tacgcggcgg
       61 cagccatgga gaggaaaccc gcccagcagg tcccggcacc atatgccgga gcagtgatat
      121 cgaaatgggt cagggcgatg atggcatcga tcgatgcgac cgggttgccc tcggggatgt
      181 gggcgacaac gaggtgggga gcaccccggc agaccggatc atagccatgc tcccatgctt
      241 tgactatcga agggaccatc ccgctcatgg ggtggttgct gtgcaggagt gttttcatcc
      301 agtctatcgt agggccggcg agcaaatgga cttcttttgg atcatgcacc accagccatt
      361 ggacaggctg tccgtttccc ccggatgctg cataccgggc aacatccaga atgcgggtaa
      421 tggtttcctt atcaacgggt tccggggtgt aatgacgcac cgaccggcgg ttcttcaggt
      481 aaatgccaat gtcttccggg gaaagggacc cggcaccggc tgccagtgaa accttttcat
      541 ccgccaggaa gttaagcgtg actgcctgtg acgggcagga cacttcacaa tgaccgcagc
      601 gtatacacat cgtggctttg gcttcgggga cacgcggaag agtgctctcg taagcagggt
      661 ctatgatttt catcgggcat acggtcgaac atatcccaca ccgggtgcag aggtcctggt
      721 caacaaaaat agtggtcatt gctttcccgt cctttccgga ttattcttcc cgtgtcctgt
      781 taaaggcttt tcttcctttc agcgaactcc cggtagaatg tttgtttatc ttgtctggta
      841 tccccggcac ctgcacaaac accgggaaaa atctgtttcc gcaggtctct gtgttaaaaa
      901 aacggaaaaa caaaggtcag atccccggaa gtgccagcgg gatcgtggtg ttatacgtaa
      961 aagggagctc aggagcaatg ccgaagaaat ccggtgatgc agtaccgctg attttgaggg
     1021 ggagttcgtg acgggaaatg atactccagc cggcagtgca catgtcggta tatctgaccg
     1081 acaccggaat tgttatcccg gtttttcccg gttttatttc gatggctccc tgctctgcat
     1141 gcgtgatagc gttccagttg gtgccgtcat ggaaccaggc atcaaaggca atttttttaa
     1201 aggggatccc gatggggttg gggttgtcaa cgtgcagggt aacgttaagg ctgatagcgg
     1261 aaagtgacag tgaagtcggg gtaacggtgg tgacacttac atccgggttt ttgaacagca
     1321 ggctgcaccc ggctacaaca acacaggaga tgataagcag gcaagcacct gctgtatggt
     1381 accatctcat actgctgtgg tttctgcagt gctcactgtt aaaacatgca ggtgtggttc
     1441 atgatgcgtg attctcaccg tgaatcccgg caaaaaaaaa ttaatctgca ccggcactgt
     1501 catagagcag ggtgatgtat cgcgcagaaa agatacctat gaacgggagc aggatgagga
     1561 acaggacaat accgaggata ggaataacaa agcagatgac ttcgataaca ccgatgatga
     1621 gcatcatgac gatcagggcg aagatgtagg taatccagcc gatcctgcca atggtagcaa
     1681 agattgcgct gaagttgaac gcttcaccaa agctgcctgt tcgggcaaaa cgcacatacg
     1741 cgatggtggc gatcaacccg ataatgagag ccacaacaag aagaaccagc actcctgcaa
     1801 gaacagcacc gatcagtccc agtaccgcac cgggatgaac ggactggcgg ggtcccataa
     1861 acagtgccag gccggcactg ccgataatga cgatttcaag gataatcacc ggcagggcat
     1921 agattattcc gacaataata agtttgatgc cgtcaataaa catggtcccc cagtcatcag
     1981 gttccggaga cggggtcacg ccacggtaaa tccgcatggt ataccccata aacaggggaa
     2041 aaatgatgca gctgataagc aaaaggaacc atttcatcca tcttccgaca agaccttcct
     2101 gagcatattc aaaagaatct ccaacagttt taccaatatc catcctcatc accttttttt
     2161 ttaaaatttt taccggttag attatctatt cctctataaa aaaacaggag gtgttttttt
     2221 gtgaggttcc cgcactattc agggattttc cctccggaag atccggttac caccactcag
     2281 atcggggttt cctggtcccc tgaggacaat ggctcttaag agatccacca gatccgggtc
     2341 cggttactcg ttccatctcc tgtacaactg gtttgcaaag acctgaaaga tcaggataaa
     2401 gacacagagc atggcgatat ctatgagcgg gttgaaatgg gtgtggccgt gataagcgga
     2461 ctggatgaga tcgttgccat aggtaagcgg tgaaaaatac gctgcaccct ggcctatgga
     2521 cggaatggac tctatcggga tgaagacacc ggagatgaat atgaggggga gcctgacgag
     2581 gtttaacatg gacatgatat ccccgggact ctcggtagga tatgcggcaa acagggtccc
     2641 catggtcgca aaacagaacg aggtgaggat gatacccata acaaggggtg caatactgac
     2701 aattggtgtg ctgaagatca gcaggccggc gataaggggg acgaacgcaa tacccatact
     2761 gtacaggaat ccgctcaggc tctcaccgag gacgactgca tgaagggata tgggggcaga
     2821 gataagccgg tcaaaggtct tcatgcggcg ttctatgggg attgataccg gttcaatcga
     2881 ggacgcggag aagagtatcg tgattgctat aagccccggg atcatgacag acggcccggc
     2941 tgaatttccg gcagcaaagg caaggaacat gaacagggga aacagtgcac cggagacaat
     3001 gatgtttggc ttgaggtaat agattgcaat atcttttttt gcaatagccc atgcagcgtt
     3061 cagctcggca tagatctgcc cgtaccagtc caacccgtca gtcaacagtg ctcacccctt
     3121 ttgtccgcac ttcaagcccg gtcagtttga taaaaacatc ttcgagactg ggaccgagcg
     3181 tgtttatact gagcacccga gtattgttca ctgcagcata ctccatgacc gcggcaatta
     3241 cctctgacgg attctgagtg aacagccgga acttgtctcc ctctttcctg acttcgctta
     3301 cctcattgag ttggcggaga tcactcccga agccggctgc tgacctgtcg aatgcaactt
     3361 cgaccgactg ggcgctctgg atggttgctt tcagtcgttc cggtgaatct atcgcggcaa
     3421 tacgaccccg gttgataatg gccacgcggt cgcaggtcag gttggcctcc tcaatattgt
     3481 gggtcgtgag aaagacggtg accccgcggg aattgaggtc ccttatcact tcccgtatga
     3541 tcaggttgct ctggacatcg agccccgatg tgggttcatc gaggaacagg agccgggggt
     3601 tattgatcag ccccattgcc agggtaagcc ggcgtttcat gccttttgag aacccgtgga
     3661 ctttgtcggt ccttcgttcc cagaggccaa acagttcaag gagctctttt ccgcgttgtt
     3721 cgcgttcttt ttttcctatg gtatagagtt cggcagtgaa catcatgttc tgccatgcgg
     3781 aaagatcatc gtagatattg gaagtttcat gcacgatccc catctgtttt ctggctgcag
     3841 tggtttcctt tacgatatct tttccaaaga ccattgcggt cccgccggta ggagcgctga
     3901 tgccggtcag gacgcggatg gttgtcgtct ttcccgcccc gttcggccca agaaaaccga
     3961 atgtctcacc ttcggtgacg gaaaaactga tatcatttaa tgcgatgagt gccccgaagt
     4021 gtttcgatag atgattgacc tcgatcgcgt acataggtac tactacttcc ggtatctgat
     4081 tttaaacgat ccttttggtc tgtccatcgg tatatatccg ggtttcttac tgaacctggt
     4141 gagaccccga tggattggtt ttgtaacatc accatgattc cggaagacgg gtgaaatcca
     4201 aggttattgg ttgtcacata tcacgaccgg gtgtcaggtc acagccggta caggaaagac
     4261 acatggtctg cgagacttcg acgtgcagat catatttttt cagaatatcc cctgtcattt
     4321 tcgtgagctt cagcaacctt tcggcgccgg ggcgtgttgc cgaaatctct cctttacgat
     4381 acggggaaat ggtgatcggg cggagaacgg caatgactcc catctttgag aggtattcaa
     4441 ctcctttcct gacacattcg tcagtctcac caagcccgat aatgatgttt gaagagaccc
     4501 ggttcttccc aaataccttt acggcatcgc gtaacgaatc gagaataaaa tcgagagagt
     4561 ggccttttct cccggtaacg acctgctcaa agatctttcg gtccatggtc tccacattgt
     4621 attttacttc cgtcacaccg gcgtctttga gccttcgtga tgagtcgtgt gtcgggtata
     4681 cggcgacccc gattggaacg ttgtatttct ttacagcctg aacatactcc acaacccggt
     4741 cgatctcgtt ttcgggcgtt gttgcgaccc ctgatgtgaa tgcgatcgtc ttcaatttcc
     4801 cgtctttttg gactttctca acgatagcaa ggacctgctc aagggtcttt atcctgccct
     4861 ggaacttggg cacagaacag aacttgcagt catacatgca ctgttcgctg atcgtgatgt
     4921 acgtctgttc ggggcagtgt gctactatgg ggtcaatttt tccccgtacc agttctttgt
     4981 catccctgat tatgacgact tccccgtcct gctgtaccat ctttaaggga gaatccgcat
     5041 cgatctcaag gcggacccgg tgacctcccg atttaaaaaa aatggatcgc agtcctgtac
     5101 cgggcccagc cgtagaacgg gaaggccgtg gtaacaacga tggttcgaca ttgacttttc
     5161 ccaaagccag caattccgct ttgatttccg catccatctg ggtcatttca ttcgttctcc
     5221 ggtgcattct tcttttttgg tacctttctt tgtattcctt atcgttatac tgcccagtgc
     5281 tcgtcgcata tcatactctg cgattatgcg ataatccggg accctggtat tatatcttga
     5341 tcccaaacag gttcgctgca ttctttccaa ggaggttctc aatcaccccg ggtttgtacg
     5401 agaaacgccg gaactcttca agactctgtt tcagcggccg taccgggtac gatgaagcat
     5461 agaggatccg gtcccctaag taatggttgg cggcctccac ccagtcctgg cctcccggaa
     5521 aaccggggat gaactggaag aagtccggca ggacccacag gtttcccaaa ggtgcattgg
     5581 caatcatgac cccgagcatt tcgctggtat agggaaatgc accgtgcaca acaacaatct
     5641 gcaggtccgg gaagtcattt gccacgcgct ggatgcgcca cggttcagca tacgagatat
     5701 cgggtccctg catcaggctg caggtgagat acaacacgcc ccccagttct gcacatttcg
     5761 catatatcgg atagatccgg ggatcatcca catacatcgg ggggacccac cagccgggat
     5821 ccacagcgat gccgttgaat cccctgtccg ttatggtacg ttcaacttca cggacagctg
     5881 cgggaactcc aatcccgttg attcctgcaa tggaaagggg aaacctgccc gggtactggt
     5941 cacggagttc aacgatgtca tcattggaga caatacggtg tcccgcctgc ctgcccacga
     6001 gtattccctt gtcaacgccg gcttcatcca tctcctgcag gaacagtaca accgatttct
     6061 gcaccatgga ctgcgggcgt tcatacccgc tcaccagagg gctgcgtttg actgacggtc
     6121 gtgctcccgg tgccggtgcc agtacatcct taaggctttt atacgggggt ctcacccgtg
     6181 catcgagtat catgtattct cctccaggga tatgttctcc gggaacctgc aggataataa
     6241 agcaggctga tcggaaattt gacagatatt gtagtatgga ccgggtatcc gtgttattgt
     6301 taaaatgacg agtgaaaaaa gaaattaatt atattgcccg tgagtgccgt aacttttgcc
     6361 cgatcgcaaa atcaattgat ttttacgcca ataccttcaa accaccggga ttcaaaattt
     6421 cccgagtaat catattcata cccgattttc ttaaaaaatg cttcaaactc tttctgggag
     6481 agggaacttt cttctgcact attttttgag cagtaaatat gcgctaaata tacaaaactc
     6541 accccatgat cagggaaatc ttcccgttgc agggaattaa tcatggggta tctcttaaga
     6601 aatttctgcc acccgggata attgcatttt acttcctggg gaaggtcttt gggtcttgga
     6661 atacaggacc cgggtgcctt ttttttacgg acaggctgct tttttattgg tgaattgaca
     6721 cgcttttccc cgttcctccg gggattgaca accggatgat cttcggggtc tgaatcctcc
     6781 gtcaccatat ggttattgag cctgtcaaaa gtataaaaaa tttgttataa ctaccaggtt
     6841 ttaaccggac agacagggtc aaaaccccgg gaaaaactca tatttccctc gcagaagtcg
     6901 ttttcggtat tgttttcaaa ctatgtttca aattttgt
//
LOCUS       DASRLV010000186         2481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_312829,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000186 DASRLV010000000
VERSION     DASRLV010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312829"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..152)
                     /locus_tag="VFC43_07185"
     CDS             complement(<1..152)
                     /locus_tag="VFC43_07185"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZK30996.1"
                     /translation="MITIDFLRYRNLAIDSLAIQPGITSVIGPNGSGKTTFLRICAGI
                     SLPETGT"
     gene            complement(149..658)
                     /locus_tag="VFC43_07190"
     CDS             complement(149..658)
                     /locus_tag="VFC43_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HZK30997.1"
                     /translation="MFGNLQRSRIIAYSAAFLGLISIGAWISVPFFPVPLTLQTLFVL
                     LAGAVMKRNAIIPVSLYMVLGALGLPVFHTGATGFGILLGPTGGYLTGFVFAALVVGL
                     FYERESATMKITGLLAGTGIIYLCGVSWLMYSLSVGFVPAVISGVLPFIPGDLMKGYA
                     VYKIAGRLP"
     gene            complement(709..1689)
                     /locus_tag="VFC43_07195"
                     /pseudo
     CDS             complement(709..1689)
                     /locus_tag="VFC43_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
     gene            complement(1694..2116)
                     /locus_tag="VFC43_07200"
     CDS             complement(1694..2116)
                     /locus_tag="VFC43_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2111 domain-containing protein"
                     /protein_id="HZK30998.1"
                     /translation="MPSYLLSCTADAGDLEPVVLAVHELIHRLPVTAKSVEKDGIRVE
                     DGRVIDSHYTGPVLLEAIERNILIKTTPTSGAYKGVPVTVSPIRDHEGKAIGAIGVVD
                     ITGIFDLATLMEHQSAILKQVCGKDPCPLPSEQSGSRK"
BASE COUNT          631 a          595 c          584 g          671 t
ORIGIN      
        1 gtaccggttt caggcagtga aatccctgca cagatcctga gaaatgtagt tttcccactg
       61 ccgttcgggc caatgacgga ggtaatcccg ggctggatgg caaggctatc aatcgcaaga
      121 tttcgataac ggaggaagtc aatggtgatc atggaagccg cccggcaatt ttatacactg
      181 cgtacccttt catcaaatct cccgggatga acggaagaac gccactgatc acggcaggga
      241 cgaaaccgac agaaagcgaa tacatcagcc aggaaacacc acagaggtaa atgataccgg
      301 tccctgccag gagcccggtt atcttcatgg ttgcagactc gcgctcatag aacagtccga
      361 caacaagggc ggcaaaaaca aaccctgtca ggtaacctcc ggtagggccg agcaggatac
      421 caaaccccgt ggctccggta tgaaacacgg ggagaccgag tgcacccaga accatgtaca
      481 gggagaccgg aattattgca tttctcttca tgactgctcc ggccagcagg acaaaaagcg
      541 tctggagtgt cagcggtacg ggaaagaagg ggacagagat ccatgcgcca atgctgatga
      601 gaccaaggaa tgctgcagag tatgcaataa tcctcgacct ctgcagattt ccaaacatcg
      661 tcaaccataa aaaaattgta cggttgacga taaaaaagtg cctcttattt atttctgata
      721 gcaatcgcca gagataaccc gttcaagttt cccgttgtct tttctgataa taagtgcgcc
      781 aaactcatcg atgtcgattg cttctccttc gaagctgttc ttcagcgtcc tgatctggac
      841 ccggttttcc agcgtgcacg agaggctctt ccactcccgg gtgattgttt cgtattcgcc
      901 actctcaacc aggagataac ggctttcgaa ctccttaagg attcgggcaa ggaatgaagc
      961 acggtcaact tcatggccga cttcagcgct gatagacgtg atatcttttt gtaatgcggg
     1021 ggagaactgg tttaacggga catttacgtc aattcctatg cttaagaggc aatagtgcac
     1081 ggaatctgcc tctgcatcga gttcaagtaa aagacccgca actttcttgt tcccgataaa
     1141 aatatcattt ggccatttga tcagggcgcc gagatcgaat tctttgcgga tagcccgtgc
     1201 aattgcaatg gacccggcca tcgtgatcat gaaaatatga tcaatcggca tacggggttt
     1261 gaggacaacg gtgatccaga tgccaccact tggagagacc taggctcttc ccattcttcc
     1321 gatcccgccg gtctgttcct cggcaatgat taccatgccg tgtatcttcc cgacatcccc
     1381 ttccaggcac atctgcttgc ccacccagat tgtcgatggg gtattttcga gataccgtat
     1441 ctttttgccg ataaactgtg tctttaactt cttgtggatc tcgtagggaa ggagcttatt
     1501 gctcgtgtgt gtgagcttat acccctcttt ttgcgatgag aagatatcat accccatcgc
     1561 ccgaagctcc tggatatgct tccagacggc agaccgggta attcccagct cgttactgat
     1621 cgcttcaccg gatatcgggg ttgttgcttt ttcgatgatt tcaagcactt tgaaagcaga
     1681 atctggcaaa agatcacttc cttgaaccac tctgttctga cggcagagga cagggatcct
     1741 tgccgcatac ctgtttgagt atcgcagact ggtgttccat cagtgttgcg agatcaaaaa
     1801 ttcctgttat atcaacaacc cctattgcac caatagcctt gccttcatga tccctgatgg
     1861 ggctgactgt tacggggact cctttatacg ccccacttgt cggtgtagtt ttaatgagta
     1921 tatttctctc tattgcttca agtagtacag gacctgtata atgactgtcg atgactctgc
     1981 catcctcaac ccgtatgccg tctttttcca ctgactttgc ggtaacggga aggcggtgga
     2041 tcaattcgtg gacagcgagg acgaccggct caagatcgcc tgcgtctgcg gtgcaggaca
     2101 acaaataact gggcataagg cgaaatcctt tcttatacac tggtcagttt ctatatagaa
     2161 taagattgcg tatacgtgga agtgttggtg gtcagaattt tcaattgaaa tacgatttat
     2221 gaaaataata ggtttggtgc agagtttttt accggcaata ttggtgaggt tatgatggca
     2281 aatcgtgtca tatcctgttg atttcaggtt ttgcctcaac caaaagagaa tatctggtat
     2341 aatttaaaag acaaagtgtt tatatcattc ttgggatacc gcgggaaatt ttcatttccc
     2401 ccaagtctgc cttttttacc gtacttcttc ctgaattctt tcctattaat cagggtattg
     2461 tttcaatatt ttgttcagtt c
//
LOCUS       DASRLV010000187         4054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_709571,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000187 DASRLV010000000
VERSION     DASRLV010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4054
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709571"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1007)
                     /locus_tag="VFC43_07205"
     CDS             complement(183..1007)
                     /locus_tag="VFC43_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZK30999.1"
                     /translation="MTETKKTVIEFPSVSDRAYKESRRHLKVDDIMSRKVITTTPETT
                     MMQAAKTMGEKHIGSLIVMKYKTPVAIVTERDLLSKVLATGKDLRKTLVGDVMSYPLI
                     TICPTFEIREAARTMINKKGRLVVYICGKLAGIITASDLIRNLPDAPETSMVVDEFMT
                     REIVSVNEDETVLAAAGIMGQQRVGSVIVTSGEKPLGIFTERDLLSTFIARSRSLDVP
                     IGEACSGLMTSAPAGISIHEAARIMAAKHIRRLPLMKKKQIAGIITARDLVEAYAK"
     gene            complement(1375..1593)
                     /locus_tag="VFC43_07210"
     CDS             complement(1375..1593)
                     /locus_tag="VFC43_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31000.1"
                     /translation="MKIKEIHMKIIALGLLPVFLILAFALPTTASKILLLGGIMVTAF
                     VVSFIGMSMGKKEEKEPDDLLAIPPKKS"
     gene            1785..2528
                     /locus_tag="VFC43_07215"
     CDS             1785..2528
                     /locus_tag="VFC43_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31001.1"
                     /translation="MDLTVFFQEQGIDVFAEVDLDNLSDEDRSSVVQFFPPARSVIVF
                     GKEVPVPVYRMPAQEKTRRMLLIAEALDNTAVRLAGYLEAGQVPAKPVPLYLPVRITD
                     GRVQGVVRLKQVAAAGGLGSPGKNTVLLNPRFGPRLLLSGVVAGRRVSESSESDTGAG
                     SEETPACSGCGRCIKVCPEGALGPEGVDTFRCRTVSAWVPPVLVPFAKWMLGRTILLK
                     CMAPLAPWIARTATIRCSLCVTECPNYSK"
     gene            2640..3380
                     /locus_tag="VFC43_07220"
     CDS             2640..3380
                     /locus_tag="VFC43_07220"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZK31002.1"
                     /translation="MKAKTMKHVNEDNREWFQEWANEYDATLGKLKRHHKLLDLVVKV
                     SGIQKNDRILDIGCGTGLLSLKFLAKTECTIMAIDSSSQMLRIFEEKIEKCHLAKKIH
                     CAQKSAEEMDFTPGQFDIIAATVSLHHVRYKEPVIKNVYDSLKGGGRFVLGEIDMDTT
                     GRLEDPRRLMRILNYLNEELALAMKEGGIPAFERMYDNGKKHILNDGEYCIGFSQWAQ
                     LCRNAGFKNVEVSPVKGFTWFKVLAATK"
BASE COUNT         1045 a          927 c         1038 g         1044 t
ORIGIN      
        1 ttgtgggatt tggtctcttt ttggggtgag gaggctctga cggcaaaata gggaagtgga
       61 tgtttcctcc tcctgtgctt taaacatgac taatcagata tattcatcat gcgttatcgt
      121 tatctccgcc agtattgccc gtttttttaa gaggatagga tttgatctct tcggacagag
      181 cattactttg cgtatgcttc taccaggtcc cgtgctgtga ttatcccggc aatctgcttt
      241 tttttcataa ggggaagccg ccggatatgt tttgctgcca tgatccttgc ggcttcatgg
      301 atgctgatac cggcaggagc tgaggtcatg agcccggaac aggcttcacc gatcggcaca
      361 tccagcgatc gtgaacgggc aatgaatgtc gagaggaggt cccgttcggt aaatatcccg
      421 agcggctttt caccgctggt aactatcacg ctgccaaccc gctgttgtcc cataattccg
      481 gctgcggcaa gaacggtttc atcttcattg acactgacga tctcccgtgt catgaattca
      541 tcgacaacca tcgaggtctc cggggcatcg ggcaggttac ggatcaggtc agacgcagtg
      601 ataatcccgg cgagcttgcc gcatatatag acgaccagcc ttcctttctt gttgatcatg
      661 gtccgtgccg cttcccgtat ctcgaatgtc gggcatatcg tgatgagcgg gtacgacatg
      721 acatccccga ccagggtctt tctcaggtcc ttgccggttg caaggacttt ggagagcagg
      781 tccctctccg ttacgatagc aacgggggtt ttgtatttca tgacaataag gctcccgatg
      841 tgtttttcac ccatggtctt tgctgcctgc atcatcgtgg tctcaggagt tgtcgtgatg
      901 accttgcggc tcatgatgtc gtccactttc aggtgacgcc tggattcctt gtaggcccgg
      961 tcggatacgc tggggaattc aatgacagtt tttttagttt ccgtcataag gatcacttca
     1021 ctgtgagaat tgagttcaag gtattaaaga ttaagtgccc tttttctttc gtccccgcat
     1081 acgatacgct cacttcccac aattaaccgt tgcccggctt aacccggagc ggagagtcac
     1141 cgtcctggag tctcctgatg ctcatagcaa tccgttattg cctgctgaca gttctttttg
     1201 tgcggcattg tcatccggtg cagcatagga atgcagatac cggccattcc cggggaaaaa
     1261 gtttgtacga agggtacact cttgatctga acagactaaa cctgaggcat gcaaaagatg
     1321 ccatgagttc aaaatcaggg atttactaac ggagatgaac aggaaaaaag aaaatcagga
     1381 tttctttggt ggaattgcca gaaggtcatc gggttccttt tcttccttct ttcccattga
     1441 cattcctatg aaagatacca caaacgcggt gaccatgata ccccccaaca ggaggatttt
     1501 gctggcagtg gttggcaatg cgaaggcgag tatcaggaat accggaagca gcccgagcgc
     1561 aattattttc atgtggattt ctttaatttt cacattcatc atcccaagtc agtcctatga
     1621 aaaacaataa ttcgtttgtt gcagaacctc atatgcgttt tgagagtgtt gtactcatct
     1681 cttataccag aacgaaaaaa agaaagggaa tataggtaaa ggaacccctt tttttattat
     1741 acttcatcaa taaggaaaga acaaatgcga agtggagtag tagattggat ctaacagtgt
     1801 ttttccagga gcagggaatc gacgtctttg ccgaagtcga tcttgataac ctgtcggatg
     1861 aagacaggtc atcagtagtg caatttttcc ctcctgcccg gtccgtgatc gtttttggaa
     1921 aggaagtccc ggtcccggtg taccggatgc cggcacagga gaagacccgt aggatgctcc
     1981 tgattgcgga ggccctggac aatactgctg tcagactcgc cggttacctg gaagcagggc
     2041 aggtcccggc caagcccgtt ccgctgtacc tgcccgtcag gattacggac ggaagagtac
     2101 agggggtagt acggttgaag caggtcgctg cggccggggg actcgggtcg ccgggtaaaa
     2161 ataccgtcct ccttaatccc cggttcggcc cacggttgtt gctgagcggt gttgtggccg
     2221 gtcgccgggt gtcggaatca agtgaatctg atactggtgc cggcagtgaa gaaaccccgg
     2281 cctgctccgg gtgcggacgc tgtatcaaag tgtgtccgga aggagcattg ggaccggaag
     2341 gtgtcgatac gttccggtgc cgcaccgtga gtgcctgggt tcctcccgtc ctggtaccgt
     2401 ttgcgaaatg gatgctggga cggacaatac tattgaaatg catggcccct cttgcgccat
     2461 ggatcgcccg gacggcaaca atccgttgca gcctctgtgt cacggaatgt ccgaattatt
     2521 caaagtgatg tggggaagag tggaacatga taacaaaacc tgaataaggc actgcacaga
     2581 ccatcaggat actgcaacgt aaaaaagaaa aaaggcaccc ggaaaaacgt accaatacca
     2641 tgaaggcaaa gacgatgaaa cacgtcaatg aggataaccg tgaatggttc caggagtggg
     2701 caaacgagta cgacgctact ctgggaaaat taaagaggca ccataaactg cttgacctgg
     2761 ttgttaaggt atcgggaata caaaaaaatg accggattct tgatattggt tgtggaacag
     2821 gtttgttgtc acttaaattc cttgccaaaa cagaatgcac aatcatggca attgacagct
     2881 catcgcagat gctcaggata tttgaagaaa agattgaaaa gtgccatctg gcaaagaaaa
     2941 ttcattgtgc acaaaaatcg gctgaggaaa tggatttcac tcctggccag tttgacatca
     3001 tagcagcaac ggtttcactg catcatgtga gatacaaaga acctgtgatt aaaaatgtat
     3061 atgactcttt aaaaggtggt ggacgattcg ttcttggaga aatcgatatg gatacaacgg
     3121 gcagactgga ggatccccgg cgattaatga gaattctgaa ttaccttaac gaggaacttg
     3181 ccctcgccat gaaagaggga ggcatacctg catttgaaag gatgtacgac aatggaaaga
     3241 agcatatcct gaacgatggt gaatactgca ttggatttag tcaatgggca caactgtgca
     3301 ggaatgccgg gtttaagaac gtggaagtca gcccggtgaa agggtttact tggttcaaag
     3361 tactggcagc aacaaaataa ccggcagaag aaaaagcaaa accaacgtca gtgtccaacc
     3421 tgagtatctt cagatcctcg ccccaaaaca ggtattcctt tccgctcgac cgtgctatcc
     3481 tccaaccctt ttaatacccg tctcccgata ggtgctttca gtggtgattt tctgcgttga
     3541 ttcagctccg gataagccaa atgagcacca gaatgaagta ttcccgaaat aagagtgtca
     3601 cttttttatg aattagtgtc attgggtgtg tgaaaatctc cttgggagaa aacagtctcc
     3661 gacagagagc tggcatggga aaagtgtagg ttttcctgag tctccggata ggaattccga
     3721 tcctcgaacc aggtattcct ttccctttga ccgtgttact ctaccctcct ccctgagttt
     3781 cgcttcctga aaacgccttt tacattacgt ttttctgctc ataatatacg tattttaact
     3841 taaaacagat aaattgcgct ttgtttgaaa acatgtgccg gatttgtggt tttgtgcaac
     3901 ctgtaccaaa aggatcgccc gtttaaacaa accggatccg atgagagcta ggatggaact
     3961 ggtcagggaa tgcagcattc cctcaacctc cagggggatc agccgtaccg gagcactaag
     4021 gggattggga catgctgcgg gaacaggata ctta
//
LOCUS       DASRLV010000188         2947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1034759,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000188 DASRLV010000000
VERSION     DASRLV010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034759"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VFC43_07225"
     CDS             <1..227
                     /locus_tag="VFC43_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31003.1"
                     /translation="PTVTTFLATLASGFTFAAIAIIFVGATILTKYKGNLWEFVVVFV
                     PIVVITAVFNAVIVQILYIPSSRVLVRGQE"
     gene            224..1672
                     /locus_tag="VFC43_07230"
     CDS             224..1672
                     /locus_tag="VFC43_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HZK31004.1"
                     /translation="MIELDNVSYTYPHTVQPALHKFSLSVKKCRCILVTGPSGAGKTT
                     LCLAAAGILHHEYGGKKEGHVAIDGRDVADYATLSELAKMIGIVFDDAEAQMIFTTVE
                     EEILSALEHRGMEAGVIEERLASIMATTFLTELKNRSPHHLSGGQKQRVALAATLALG
                     NDVLILDEPTSELDEQATRRIVEILNTLKTQGKTLLLVEHKYAHFHDMVDTLVVLESG
                     TIVAKGSPEDVLKDDRIKKIVIPDFSGVRGELQKTGIPERKDKEPVVQVQHLSYAYGD
                     VLALNDISLDIHAGEFVALVGENGSGKTTLVKHFNRLLSPTSGDVVIQGKNTKTSTIT
                     DLARHVGLVFQNPDHMFFADTVKDEIEFGLKNLGVTDGDAIIDAALNDVGLVHTKDLY
                     PRWLSRGERQRLAIACVIAMSPSIIVLDEPTTGLDGNEARLVMEILKNLQAKGHTIIV
                     ITHNKEIAEHCADRTITMEMGKIISDSTIGGT"
     gene            1673..2467
                     /locus_tag="VFC43_07235"
     CDS             1673..2467
                     /locus_tag="VFC43_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HZK31005.1"
                     /translation="MAEILQYVHKDGFFHRLTPFSKIAFIIVISLMCIISINIFFLAG
                     LVIALLIIAWVSRLHREVLQQSKLILVMSGVFIIITLITMPNGAIIGYLIPEGVPLLG
                     GSIPVTQGAIEIGLILTFRFMILIFAFQMFLLSTQPRDLVHTLEKMRMPIDYILMFII
                     ALRFIPTLQIEGNRIHEAQLARGYNPGTGFIGKIRSIAPIVIPLVSNALLRSTVLGLT
                     IDMRGYRTGKRTHVRESTLKSRDYGVLFAMGLASCGYIVLFMKQVI"
BASE COUNT          759 a          720 c          725 g          743 t
ORIGIN      
        1 ctcccacggt tacgacattt cttgcaacac tcgcaagcgg gtttacgttt gccgcaattg
       61 caattatctt tgtaggagct acaattctta ccaagtataa gggaaatctc tgggaatttg
      121 ttgttgtatt tgtccccatc gtggttatca ccgcggtgtt taatgcggtt atcgtccaga
      181 tcctctacat cccctcaagc agggtgctgg tgagaggaca ggaatgatcg aactcgacaa
      241 tgtcagttac acctatcccc acacggtgca gcctgccctg cacaagtttt ccctttcggt
      301 aaaaaaatgc aggtgtattc tggtgaccgg tccgtccggt gccggaaaaa caacactgtg
      361 ccttgctgca gccggtatcc tgcaccacga atacgggggg aaaaaagaag gtcatgtcgc
      421 cattgacgga agagatgtgg cagattatgc cacgctgtct gaactggcaa agatgatagg
      481 aatcgttttt gatgatgccg aagcccagat gattttcacg acagtcgaag aagagatact
      541 ctctgcgctt gagcaccggg gaatggaagc aggggtcatc gaagagcgtc tcgcatcaat
      601 catggctaca acgttcctta ccgagctgaa gaaccgttcc cctcatcatc tctcaggagg
      661 tcagaaacaa cgcgttgctc tggctgcaac acttgctctt ggaaacgatg tccttatcct
      721 ggatgaaccc acttcagaac ttgatgaaca ggccacccgg cggattgttg agatcctgaa
      781 taccttaaaa acacaaggaa agactctcct tctggttgag cacaaatatg cacatttcca
      841 tgatatggta gacaccctgg tcgttctcga atcaggtaca atcgtcgcga agggatcacc
      901 ggaagatgtg ctgaaagatg atagaataaa aaagatcgtg ataccggact tcagcggtgt
      961 gcggggcgaa ctccaaaaaa caggcattcc ggaaaggaag gacaaggaac ccgttgttca
     1021 ggtgcagcac ctctcttacg catatgggga tgtcctggcg ctgaatgata tcagcctcga
     1081 tatccatgcc ggagaatttg tggcacttgt cggggagaat ggatcgggaa aaacaacact
     1141 ggtcaagcac ttcaaccgcc ttctctcacc cacgagcggg gatgttgtaa tccagggaaa
     1201 aaatacaaag acctcaacaa ttaccgatct tgcccggcat gtcgggctgg tcttccagaa
     1261 cccggatcat atgttttttg ccgatacggt aaaggatgaa attgaattcg ggctgaaaaa
     1321 ccttggagta accgatggcg atgcgataat cgatgcagca ttgaatgatg tcgggcttgt
     1381 gcacaccaaa gatctgtacc cccgctggct ctcccgggga gaacggcaga ggcttgccat
     1441 tgcgtgtgtc attgcgatgt caccctcgat tattgtgctg gatgaaccaa caacgggtct
     1501 tgatggcaat gaagcccggc ttgtcatgga gattttgaaa aatcttcagg ccaaggggca
     1561 tacaatcata gtaatcacac ataataagga gattgcagag cattgtgctg atcgcactat
     1621 tacgatggaa atgggaaaaa taatttcaga ctccacaatc ggggggacgt aaatggcgga
     1681 gatactgcaa tacgttcaca aggacgggtt ctttcaccgg cttactccgt tttccaagat
     1741 cgcgttcatc attgtcatca gcctcatgtg catcatttcg ataaacatat tttttcttgc
     1801 gggacttgtt attgcacttc tcattattgc ctgggtaagc cggcttcatc gtgaagtgct
     1861 tcagcagtcg aagcttatcc ttgtcatgag tggagtgttt atcatcatca cgctcatcac
     1921 catgccaaac ggagctatca tcggttacct catacctgaa ggggtacctc ttctgggagg
     1981 aagcatcccg gtcacccagg gggctattga gattggtctt atccttacgt tccgttttat
     2041 gatccttatt tttgcattcc agatgttcct gctgtcaacc cagccacggg accttgttca
     2101 cacactcgaa aaaatgagga tgccgataga ttatatcctc atgtttatta ttgcgctccg
     2161 gttcataccc actctgcaga ttgaggggaa ccggatccac gaggcgcagc ttgcccgtgg
     2221 ctacaacccg ggaaccggct ttatcgggaa gatccggagc atcgctccca tcgtaatccc
     2281 gctggtctca aacgctcttc tcagatccac ggtgctgggt ctcactatag atatgcgggg
     2341 ataccgtaca ggaaaaagga cgcatgtgcg ggagtcgacc ctcaaatcca gggattacgg
     2401 ggtgttgttc gcgatgggtt tagcctcctg cggatatatt gtgcttttca tgaaacaggt
     2461 aatctgaaaa aaaatccgat ttcaaccact tttttaaaaa atctgctctg tagaattcct
     2521 ctttagccct gatttctcat ggggaggatg ctggaatatc ccacggcgcc aatgcccgcc
     2581 gcgtcccaaa ccacacccca tcctgctttc gatcttcgta acattgctat atccgacctc
     2641 atccgttccc gtcggaaatc gttgaataat tccggcccgg taccgcattt ttccgaatta
     2701 tgacctctga aattcggggg gtgggggaaa tgaggtataa ccgggctcgt agcaggctcc
     2761 ggtgtaccag aattctcacc ggggtttttt gacggtaaaa atagctccag atggcaataa
     2821 catccggcct agtcttcggt gagattgctc ctttaataca ggatttctac aaagcaaaaa
     2881 aattcagaaa aacaagcgcc tcggccggga tttgaacccg ggtcaaaagc tccggaggct
     2941 tctagga
//
LOCUS       DASRLV010000189         4558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1126595,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000189 DASRLV010000000
VERSION     DASRLV010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1126595"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..645)
                     /gene="ruvA"
                     /locus_tag="VFC43_07240"
     CDS             complement(49..645)
                     /gene="ruvA"
                     /locus_tag="VFC43_07240"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HZK31006.1"
                     /translation="MIAHLRGEAVLTGDRWVVIDVGGIGYQVQVTQPELHLIGMSQEK
                     VKLYTYMAVREDAITLYGFLHHSELEMFKILIGVSGIGPQTAMNLLSQIGIEDFAIAI
                     LNEDEKALTRISGIGTKSAKRLILELRDKMKKISETMMCSDAGRVVPAVHDAVSALVS
                     LGFCEKESREAVHAGSLQLSSPTVQDLIKAALSRLKEH"
     gene            complement(650..1123)
                     /gene="ruvC"
                     /locus_tag="VFC43_07245"
     CDS             complement(650..1123)
                     /gene="ruvC"
                     /locus_tag="VFC43_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HZK31007.1"
                     /translation="MIVIGVDPGLARLGYGVIEVSRGEIQPVCYGIIETSGKDHRASE
                     RLLKIYTEIGTLFDKYTPNHISLEKLFFTKNISSAMSVSEVRGIILLAAEQRLIPVTE
                     YTPNQIKQAITGSGRADKRQVQEMIKRLLHLDEIPTPDDAADALSIALCHIHIMR"
     gene            complement(1232..2179)
                     /gene="mch"
                     /locus_tag="VFC43_07250"
     CDS             complement(1232..2179)
                     /gene="mch"
                     /locus_tag="VFC43_07250"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HZK31008.1"
                     /translation="MLSVNELALEIFENLAEIPEEFNCTYHELDNGARIVDCGVSTRG
                     GYAAGRAFTEICMGGLGEVNFRMGHIKEFPMPFIDVMTDFPSISCLGSQKAGWTVKVG
                     NYFAMGSGPARALSLKPKHTFEVIEYEDDFDWAVICLESDHLPNAEVMGKIAEECNVE
                     PANVCAVVAPTSSIVGSIQVSGRCVETAIYKLNELGFDTRKVIAAIGTAPVPPVRGAK
                     LAMGVTNDATIYHGQINLTMKAPEIKEYLEKIPSNKSKGYGKPFNDIFKEAGYDFYKI
                     DTALFSPAEVIINELSEGVVYHIGAVNTDVTLKSFGLQK"
     gene            complement(2247..3371)
                     /locus_tag="VFC43_07255"
     CDS             complement(2247..3371)
                     /locus_tag="VFC43_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORC1-type DNA replication protein"
                     /protein_id="HZK31009.1"
                     /translation="MKKNLLMWDETLFRDPEVLEIDYVPEQFEFRDAQMRELAFQIRP
                     GLRGGRPLNTVCKGLPGTGKTTSIRKLFTEIEETTKKLVPVYINCQIDNTKFAIISQI
                     YKKLSGHLPPSSGTSFKQVFDAVARILIKDEIVLLVALDDANYLLYENEINKVLYTLL
                     RSHETYEGTRIGVIVIVSDLDVDLNRAVDARVASVFRPTEIYFPPYDTTEIQEIMKAR
                     VVQGLFQGVLSEELLNLVVEQTLKSGDLRVGIDLLKRATLSAERAAKRSIERVDICGA
                     YEISKYLHLSYTIKTLKDEERLILKSCAERSEKDREMNAGDVYKSIKESLSIGYTRFY
                     EIIKKMDAMRLINLEYRDGKGRTRIITLRYDPAKVLEYLG"
     gene            3895..>4558
                     /gene="kdpC"
                     /locus_tag="VFC43_07260"
     CDS             3895..>4558
                     /gene="kdpC"
                     /locus_tag="VFC43_07260"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HZK31010.1"
                     /translation="MIRKQAKPALVIFFLLTILTGIIYPLVITGIAQLVFPVQSNGNL
                     IEHNGKIVGSSLIGQPFTSSKYFWGRLSSTSPVPYNSGVSGGSNLGPTNPALLDEVKA
                     RVNALHSADPDNSQPIPVDLVTSSASGLDPDISVAGAKYQVPRIARERNMSESDVNAL
                     VEQQTEPQQFDLFGEPRVNVLKINLALDDLSAHRITVTPSAGFEDVPSSLLFGLRMTD
                     WVQ"
BASE COUNT         1138 a         1118 c         1039 g         1263 t
ORIGIN      
        1 cctcttctgt cagcggatcg ggtgagatta tcctttcagt cattgtcatc agtgttcctt
       61 aagtcttgaa agagctgcct tgatgagatc ctgaactgtc ggagagctga gctgcagaga
      121 tccggcatgt acggcctccc gggattcttt ttcacaaaat cctaaagaaa cgagggcact
      181 gacagcgtca tgtactgctg gtactaccct ccccgcatca ctacacatca ttgtctcgct
      241 gattttcttc attttatccc tgagttcgag gataagtcgt ttggcactct tggtgccgat
      301 accagagatc cttgttaagg ctttttcgtc ttcattcagg attgcgatgg cgaaatcctc
      361 tatcccaatc tgggataaga gattcatggc agtctgtgga ccaatcccgc ttacaccgat
      421 caggatctta aacatctcca gctcactatg atgtaaaaat ccatagagcg ttattgcgtc
      481 ctcacggacc gccatatacg tataaagttt aactttctcc tggctcatgc ctatcagatg
      541 gagttccggc tgggtcacct gtacctgata cccgatgccc ccaacatcga tcaccaccca
      601 tctgtcgccc gttaatactg cttcaccacg gagatgtgcg atcattgtac tatctcatga
      661 tatggatatg acataaggca atcgaaagag catccgcagc atcgtcaggc gtaggaattt
      721 catcgagatg gagcagtcgt ttgatcattt cctgtacctg acgcttgtcg gcacgcccgg
      781 aacccgtgat tgcctgcttg atctggttag gggtatattc agtaaccggg atgagacgtt
      841 gttcggccgc aagaaggata atcccgcgaa cttcgctcac actcatggct gaggagatgt
      901 ttttcgtgaa aaataatttt tccagggata tatgattcgg ggtgtatttg tcaaaaaggg
      961 ttccgatctc cgtatatatt tttaataatc gctccgatgc acggtgatct ttaccggagg
     1021 tttcaataat tccgtaacag accggttgaa tctctcctct ggaaacctca attacaccat
     1081 atcccagtcg ggcaagtccc ggatctaccc caataacaat cattccaact tctcatgagg
     1141 tatatgtcgc tcctccatta aacggcaaac ggttggcggt gtgaaggtga gatttgaggc
     1201 aggtaacacc aaaaaagatt aagcgggtat gttacttttg taatccgaat gatttcaggg
     1261 tcacatcggt gttaactgca ccgatatgat acacaactcc ttcggacaat tcattgatga
     1321 taacttctgc aggtgaaaaa agcgcagtat caatcttata aaagtcatat ccggcttcct
     1381 tgaagatgtc gttgaacggt ttgccgtagc ctttagactt gttgctgggg atcttctcga
     1441 gatattcttt aatttcgggg gctttcatgg tcaggttaat ctgcccgtga tagattgtgg
     1501 catcgttggt gacgcccatg gcaagctttg cgcccctgac cggtgggacc ggtgcagttc
     1561 ctattgcagc aatcactttt ctcgtatcga aaccgagctc attgagctta tagattgcgg
     1621 tttcgacaca acgtccggaa acctggatcg agccaactat tgaagacgtg ggtgcgacga
     1681 ctgcacagac attagccggt tcgacattgc attcttctgc aatttttccc atgacttccg
     1741 cattgggaag gtgatcgctc tcaaggcaga tgacagccca gtcgaaatca tcctcatatt
     1801 caatcacttc aaaagtatgc tttggcttga gagagagtgc ccgtgccggt ccgctaccca
     1861 tggcaaaata gttaccgact ttgaccgtcc aacctgcttt ctgtgaaccc agacatgaga
     1921 tggacgggaa atccgtcata acgtcgatga acggcatggg gaattctttg atgtgcccca
     1981 tccggaaatt tacctcgccc agtcccccca tacaaatttc ggtgaatgcg cggccagcag
     2041 cataaccccc gcgggtgctt acaccgcagt caacaatccg tgcaccgtta tcaagttcat
     2101 ggtaggtaca gttgaattct tctggaattt ctgctaaatt ctcaaatatt tccagtgcca
     2161 gttcattaac actcaacatc ttacaaaacc cctgcatcat aaaagagggt ctgggaacag
     2221 ataatatttt tcaaactgat ccggcgtcat ccgagatatt cgaggacctt tgccgggtcg
     2281 taccggaggg ttatgatccg ggttctgcct ttcccgtccc ggtactcaag atttattaac
     2341 cgcatcgcat ccattttttt aataatttca tagaaccgtg tgtatccgat cgacagggat
     2401 tctttgatcg atttgtatac atcaccggca ttcatctcac ggtctttttc acttcgttct
     2461 gcacacgatt tgagaatcag ccgttcctca tccttgaggg ttttaatggt ataggacagg
     2521 tgaaggtact ttgagatctc gtacgcacca cagatatcaa cacgctcgat actgcgcttc
     2581 gctgcacgtt ctgcactgag tgtagcccgt tttaaaagat cgattcctac cctcagatct
     2641 ccgctcttca gggtctgttc tactacgaga ttgagcagct cttcactcaa aacaccctgg
     2701 aaaagaccct gcactacccg tgccttcatg atttcctgaa tttctgtagt atcatacggg
     2761 ggaaaataga tctcggtggg gcggaaaacc gaggcaacgc gggcatcgac tgcccggtta
     2821 agatcgacgt caagatcgct gacaatcaca attaccccga tccttgtccc ttcgtaggtt
     2881 tcatgtgacc ggagcagggt atagagaact ttgtttatct cgttttcata gagaagataa
     2941 ttcgcatcat cgagcgcgac aagtaaaacg atctcatctt ttatcaggat acgggcgaca
     3001 gcatcgaata cctgcttgaa ggatgtaccg gatgaagggg ggaggtgtcc tgacagcttt
     3061 ttgtagatct gtgatattat tgcaaatttt gtattatcga tctggcagtt tatgtataca
     3121 ggaacgagtt tttttgtcgt ttcctcgatc tcggtgaaaa gtttacggat gctcgtggtc
     3181 tttcctgtgc ctggtaaccc cttgcatacg gtattgagcg gcctgccccc gcggagtccc
     3241 ggccggatct ggaatgccag ttcgcgcatc tgcgcatcac ggaattcaaa ctgctccggg
     3301 acataatcaa tctccagtac ctcggggtcg cggaagagtg tttcatccca catcagcagg
     3361 ttctttttca tctcactaca cctctacgcc atcggtatta actgcattgg attatttcct
     3421 gactttaaag atttggtttt ttcgttcgat cactttgcgg tgacaaaaat atatctccag
     3481 ataaagaaaa agaaccagac agctgcgaca gatgatgaaa gttaccccat ctaatattat
     3541 aggaagtaac tattcatttt tagtgaggga aacaactggt atacagcccc tttttttgat
     3601 gaggcaggag agaaatttga atggagatgt ttgaaacgct gatggcgcct ttacctgctt
     3661 tctgtgcgat gtgtgtaatt tatatactaa tgtaaggaag tattcttcca ttgaatttga
     3721 ttagctgatg ttgtatagtt ttcggacaag tgcgtttcgt acttttcacg agagcgaaat
     3781 tctcataggg attaaatatc ggcaaccagc acgctggtac agtaaattct agaggaatca
     3841 ggagtgcaat acgggtaagg cggtcaatga atgacaactg aaccgattca aaccatgatt
     3901 agaaaacagg caaaaccagc actcgtgatc ttcttcttac tcaccatcct tactggtatc
     3961 atctacccgc ttgtcattac gggtatcgca cagctggtct ttcccgtaca atcgaatggg
     4021 aacctaatcg aacataacgg gaagattgtt ggctcttctc tgatcggcca gcccttcact
     4081 tcatcaaaat acttctgggg gcgcctctca tcaacaagtc cagtgcctta taattccggt
     4141 gtctcaggcg gatcgaacct tggccccacg aatccagcac tcctggatga ggtgaaggct
     4201 cgtgtgaacg cacttcattc ggccgacccg gataattccc agccgattcc cgttgacctt
     4261 gtcaccagtt cagcgagcgg ccttgatcct gatatcagtg tagccggagc gaaataccag
     4321 gtcccccgaa tcgcacgtga aaggaacatg agcgagagcg acgtgaatgc actcgtagaa
     4381 cagcagactg aacctcagca gttcgatctc ttcggcgaac cgcgggtgaa cgtcctgaaa
     4441 atcaaccttg cactggacga cctgagtgca catcgcataa ccgtaacacc atctgccgga
     4501 tttgaggatg tgccctcatc gctcctcttt ggtctcagga tgactgactg ggtccagc
//
LOCUS       DASRLV010000190         2967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1036572,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000190 DASRLV010000000
VERSION     DASRLV010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036572"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VFC43_07265"
     CDS             <1..228
                     /locus_tag="VFC43_07265"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HZK31011.1"
                     /translation="QYPFISDCSEGESVIIHCIQGKACSKRLLDLGMVPGEQVSIKRK
                     LFNGALVVQVKGCDVALSPEIASAIGVERCA"
     gene            225..2075
                     /gene="feoB"
                     /locus_tag="VFC43_07270"
     CDS             225..2075
                     /gene="feoB"
                     /locus_tag="VFC43_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HZK31012.1"
                     /translation="MKIALLGNPGAGKSLIFNQLTGLGIEVNRYPGSSVCLECGNVCY
                     KKEKIEIIDLPGIYSLDGSSEEEVLVRKFLEEAKADIIVAVLDATKLERNLYLFNQVT
                     EFSPRLIVVVNKIDEAERQGLIIDSDKLSVLLGAAVLLTAASEGRDIDRILPLALGSA
                     KAANLKVPYDHHIEAALNSLEKTLRTPRYKGLLALQGMTEDHMLLDSAGAIAHEIELR
                     HRMSVRQIIATNRHNLARKVAAAAVSHGDRKKTYDLDSLLTRVFPGVPILIAILFFLL
                     LSVFVVGSWMEGIIVGSFTRLVIAPFTALALPPLWERIGFSMLIALQAGFGIAFPFIF
                     TFYLGLSLLEDTGYMTRAAFLADRAMHRLGLHGEAIIPMVIGFGCTVPAVMSLRILKT
                     RREQTIAAFLISMVPCSARTVVIAGIVAAFVGIFWAFSIYLIVFVLIIITAIILTRIT
                     PGEQFGMIVEMSEIRRPRARLVIAKSWLRIKEFLFIAMPLLIITSIALGLFQYFGLTD
                     LFQQLVAPITITLLGLPAYASTALLFGIFRKELAFETLAVLAGTADLGSVMTSVQLYT
                     FAIVSVLFVPCTSTIAVLYRQLGARITALISVYTVFLGLLIGAVINLIMK"
     gene            2200..2949
                     /gene="dph5"
                     /locus_tag="VFC43_07275"
     CDS             2200..2949
                     /gene="dph5"
                     /locus_tag="VFC43_07275"
                     /EC_number="2.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004164 - diphthine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0017183 - protein histidyl modification to
                     diphthamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine synthase"
                     /protein_id="HZK31013.1"
                     /translation="MLTFIGLGLYDKTDVSEKGLTAIRHADHVFLETYTSRLMGTSHE
                     ELQGHYGKPVNLLGREDVEIHPENILAKAEHEQVVFLCAGDPMVSTTHADLRMRAALR
                     GIKTAIIHAASISSAVCGLSGLQNYRFGKSCSVPFPQKNWFPKTSIDVIAQNLSLRLH
                     TLVYLDIQDERYMTVQEAVVLLEQMAEEVKIHIPLYVGIARAGSDDPLVRAGSAQVVR
                     SVDFGPPLHILVVPAELHEMERTYLEMFAGL"
BASE COUNT          672 a          774 c          764 g          757 t
ORIGIN      
        1 cagtatccct tcatttctga ttgttccgaa ggggaatctg tcatcatcca ctgtatacag
       61 ggaaaagcgt gttccaagcg cctcctcgac cttggtatgg tccctggtga acaggtgtcc
      121 ataaaacgca aattattcaa cggcgctctg gttgtgcagg tcaagggttg cgatgtcgcc
      181 ctgagccccg agatcgcgtc tgcgattggt gtggagcgct gtgcatgaag attgcactcc
      241 tgggaaaccc gggtgcaggc aaatccctca tcttcaacca gctgaccggg cttggtatcg
      301 aagtgaaccg ctatccggga tcctccgtct gcctggaatg cggtaatgtc tgttataaaa
      361 aagagaagat cgagatcatc gacctgcccg gaatttattc ccttgacggg agttctgaag
      421 aagaagtcct ggtgcggaaa ttcctcgaag aagcgaaggc agatatcatt gttgcagtcc
      481 ttgatgccac aaaacttgaa cgcaacctgt atcttttcaa ccaggtcact gaattttccc
      541 cccggctcat tgtcgttgtc aataagattg acgaggccga acggcagggg ctgatcatag
      601 acagtgacaa gctctcagta ctgctcgggg ccgctgttct tctgactgct gcatcagaag
      661 gaagggatat cgaccggatc ctcccccttg ccctgggatc ggcaaaagca gcaaacctga
      721 aagtacccta cgaccaccat atcgaagctg ccctgaacag tcttgaaaag acactaagaa
      781 ccccacgcta caagggcctg cttgccctgc agggtatgac cgaagaccat atgctccttg
      841 attcggcagg agctatcgcg cacgaaattg aactgcgcca ccggatgtca gtccgccaga
      901 tcattgcgac aaaccgtcat aacttagccc ggaaggttgc tgcagcagcg gtcagtcatg
      961 gtgaccggaa aaaaacctat gaccttgaca gtctcctcac ccgggtattt ccgggagtcc
     1021 ctatccttat tgccatcctc ttttttttgc tcctgtcagt ctttgttgtc ggctcctgga
     1081 tggaagggat aatcgtcggg tcattcaccc ggctggttat tgccccgttt actgccctgg
     1141 cattgccgcc cctctgggaa cggattggtt tttcgatgct tatcgccctg caggcaggtt
     1201 ttggtattgc attcccgttt attttcacgt tttacctggg actctctttg cttgaagata
     1261 cgggatacat gacccgtgcg gcattccttg ctgaccgtgc aatgcaccgg ctcgggctgc
     1321 atggcgaggc aatcatcccc atggtgatag gctttggatg cacggtgcct gcggtgatga
     1381 gcctccggat cttaaagacg cggcgggaac agacaatagc agcgtttctc atcagtatgg
     1441 tcccctgctc ggcaaggacg gttgtgatag caggtatcgt tgctgccttt gtcgggattt
     1501 tctgggcatt ttctatctat ctcatcgtct ttgtcctgat tattataaca gccatcatac
     1561 tcacccgcat aacacccggt gagcagtttg gcatgatcgt cgagatgtcg gagatccgca
     1621 gaccacgggc gcgactggtg atcgcaaaat catggctccg gattaaagaa ttcctgttta
     1681 tcgcgatgcc gctgttgatc atcaccagca tagcgctcgg actgttccag tatttcgggt
     1741 tgaccgatct tttccagcag ctggtcgctc ccataaccat aaccctcctt gggttgcctg
     1801 cctatgcatc taccgcattg ttattcggca tcttcagaaa ggaactggct tttgagaccc
     1861 tcgcagtcct tgccggtaca gctgaccttg gttcggtgat gacttctgtc cagctctaca
     1921 catttgccat tgtgagtgta cttttcgttc cctgtacttc cacgattgcg gtattatacc
     1981 ggcagctcgg ggcacggatt actgccctga tatcagtata tacggtattc ctggggttgc
     2041 tgataggagc ggttattaac cttatcatga aatgatcaag cgatacatgt accggtacct
     2101 ccgggaatta caaaagagct gactcccctg aattggttca cgtgatgaaa aaggagaact
     2161 tgtccactgg accctgtggc tgaacaaagg aggaatatat tgctgacatt tattggcctc
     2221 ggactctacg ataagacaga tgtttcagaa aaagggctca ctgctatccg gcatgcagac
     2281 catgtatttc ttgaaacgta cacgtcccgg ctcatgggta catcgcatga ggaactccag
     2341 ggacattatg gaaagccggt gaacctgctg ggccgtgagg atgttgaaat ccaccccgaa
     2401 aatatcctgg caaaagccga acatgaacag gtggtatttc tctgcgccgg agaccccatg
     2461 gtctccacta ctcatgctga ccttcggatg cgggcggcac tcaggggaat taaaaccgcg
     2521 attattcatg cagcatccat ctcaagtgct gtctgcggac tttcggggct ccagaactac
     2581 cggttcggaa agtcctgctc ggttcccttc ccgcagaaaa actggttccc gaagacctcc
     2641 atcgatgtta tagcgcagaa cctgtccctc cggttgcata cgcttgtgta ccttgatatc
     2701 caggatgaga ggtatatgac ggtccaagaa gctgttgtcc tgcttgaaca gatggcagaa
     2761 gaggtaaaaa ttcacatccc cctgtatgtc ggtatcgcac gggcagggtc tgacgaccca
     2821 ctggtacggg cgggatctgc tcaggtagtg agatctgtgg atttcggtcc gccccttcac
     2881 atactggtag tccccgcaga actgcatgag atggaacgga cgtacctgga gatgtttgcc
     2941 ggtttatgaa gattgcagaa tgccagg
//
LOCUS       DASRLV010000191         4327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_316292,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000191 DASRLV010000000
VERSION     DASRLV010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316292"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            624..2078
                     /locus_tag="VFC43_07280"
     CDS             624..2078
                     /locus_tag="VFC43_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HZK31014.1"
                     /translation="MPHICIGFEVHQPYRLNRTFSPNPKIKKKDLFDHYFDSLNKEIL
                     LRVVDKCYDPATKLILEKLDEGFACSFSMSGILIEQLEKWSRDTLSLFEEVARHKNTE
                     MIGQTYYHSIASCFSDKSEFRDQVKLHSDLMHDLFGVRPTIFENTEFTFNNEVAATIR
                     DLGFAGIFTEGVDRILDWRSPDYLYSCRDIPVILRNTKLSDDIAFRFANRTWDMYPLT
                     ADTYAHWVASSSGDIITVFLDYETFGEHFWQETGIFDFLRALPAELSKRDVGTILPSQ
                     VVSQYSPVGEIDVQETISWADLEKDTSAWMGNDHQQTAFHAVQSARAYAVNKPLWRYL
                     QTSDHFYYMASKYGTCGEVHTYFSHHVAEDAFQTYMSIIADYESRNIRVMKNRKSAKI
                     LRTLPPDQAFHFASPAGFIGHTAYNLDQFEELLYFVPRDSLNFHQDRGDFVRWITDVL
                     DDPELAGNINGLTERHDLTSVIKERRELLWSHLK"
     gene            2099..3202
                     /locus_tag="VFC43_07285"
     CDS             2099..3202
                     /locus_tag="VFC43_07285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZK31015.1"
                     /translation="MYAERVGGLASAATHLAETLVNQNHEVHFFTRGNISDQVIKGVH
                     YHYCQPSGKNIVEFCDSMSASMVDQFYRYDRPHRFDVLHFHDWHPVQALHHLQDRNTI
                     LSFHSTEYGRFGNQFGDWWEFKEISGKEWYGGLIAKRVTAVSETLKKELMQLYNIPDE
                     KCDVIPNGVIPREFRSAIDPGEVKKSYGIHPYAPLILFIGRLVYQKGPDLLVEAIRKV
                     CQHRWDAQVVVAGEGGMRQFLEERAKDLPVNFVGYIPDSEYVRLLNATDIVVIPSRNE
                     PFGLVLLEAWSAEKCVVASNVGGLSENIDAFENGIKVDVDPESLAWGISMMIDEPENA
                     GAMGKRGRAKVDRLFLWDPIVRGLTATYARTVA"
     gene            3344..4327
                     /locus_tag="VFC43_07290"
     CDS             3344..4327
                     /locus_tag="VFC43_07290"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HZK31016.1"
                     /translation="MTLKELSMHLKNFKDTIAASPCIFTLGVAGDSGSGKTTFTQGIR
                     SIFGDDLVSTITLDDYHSLDRAGRTEQGITALDPRANRIEQLERDLILLKQGMQIEKP
                     VYNHATGTFDPPVLFRPKKILILEGLHTLFTPTLRKCLDFTLFVDPAQQVKYDWKIRR
                     DMEKRGYSHDAVMKEITEREPDYQRYIAPQKEFADAVIGIDYSEFGQDLGLERNVYRI
                     TLSQNRMNHAMEDIDLSLDLYSILSLSQRNFSLKFLTGHHNNHRIGQLVMDGELREDV
                     GEKLERSIENQTRVHPISLFRQRDYVPAGDLAQLILCWRIIHRRIFMETSR"
BASE COUNT         1146 a         1045 c         1083 g         1053 t
ORIGIN      
        1 ggggagaaag gtgtcaccct cctgcatgct gatgagaata agtgaagggt atagggtcat
       61 ttcaaccacc aactttttta aaatattcga cgggaaaaaa agaataattt gataaccaga
      121 ataattttca aaaaagtgaa acgttgattt agataaaata aatccggagt aggtttaccg
      181 aactcgttct atcgcatctt ggggacgcca cagcggcggg gacgagctaa aagcgagtcc
      241 ccaggagcgt ctgagtgagg ctttttattc aacacatccg aaatttttag tcggtagaat
      301 gtgactatac cctaagtgaa taatgaaaaa aaaggatttc aacaaagccg ttttgtcttt
      361 tatttaaaaa aaacaaggtt ttctcccggg caaaaaattt cactctcaaa aattgtaaac
      421 aaagaaaaat gttatatctt ctctcgagta tcatctctgt gactaaagga acagtattcg
      481 tatcccccgg aacccgcgtg cggaatcctc tggtgatttt ttatcctgat accggttagc
      541 agcaggcgca gaggatctca aacggttttt ttggttccgg taaggctctt taggtcggta
      601 gtaaaatgga ggtgaataaa aatatgccac atatctgtat aggatttgaa gtccaccagc
      661 cttaccggct gaaccggacg ttctcgccca atccaaagat taagaaaaag gatctgtttg
      721 atcattattt cgacagtctg aacaaggaaa tcctgttacg tgtcgtagat aagtgctatg
      781 atcctgccac aaaactcatc cttgaaaaac tcgatgaagg atttgcctgc tcattttcga
      841 tgtcgggcat tctcatagag caactggaga aatggagcag ggataccctg tcgctttttg
      901 aggaggtagc acgacacaaa aacacagaaa tgatcgggca gacctactac cacagcattg
      961 ccagttgttt ttcagataaa tcagaattca gggaccaggt gaagttgcat tcagatctga
     1021 tgcatgacct gttcggtgtc cgtccgacca tttttgaaaa taccgaattt acgtttaata
     1081 acgaggttgc tgccacgatc agggatctgg gatttgccgg gatttttact gaaggagtag
     1141 atcgtatcct ggactggagg agccccgatt acctgtattc ctgcagggac atacctgtaa
     1201 ttctccggaa tacgaagttg tccgatgaca tcgccttccg gtttgccaac cgtacctggg
     1261 acatgtatcc gctgactgct gacacctatg cccactgggt tgcatcatct tccggggata
     1321 tcattacggt attccttgac tacgagacat ttggtgaaca tttctggcag gagaccggca
     1381 tattcgactt tttacgcgcc ctgcccgcag aactcagcaa acgggacgtc ggaacgatcc
     1441 tgccatcgca ggttgtttcc caatattctc ccgtaggaga aatcgacgtt caggagacca
     1501 tttcatgggc tgatctcgag aaggatacgt ccgcatggat gggaaatgat caccagcaga
     1561 cagcgtttca tgctgtgcag tcggcacggg catacgcagt caataaaccc ctgtggcggt
     1621 atctccagac cagtgaccat ttctattaca tggcatccaa atacggcaca tgcggcgaag
     1681 tccacaccta cttcagccac catgtagcgg aggatgcgtt ccagacgtac atgagcatca
     1741 ttgcggacta cgagtcacga aatatccggg tgatgaaaaa ccggaaatcg gcaaagatat
     1801 tacgaaccct gccccctgat caggcatttc attttgcgtc tccggccggg tttatcggcc
     1861 atactgccta caacctcgac cagtttgaag agctcctcta ttttgtgccc cgggattctc
     1921 tcaactttca ccaggacagg ggtgattttg tcaggtggat taccgatgtg ctcgatgatc
     1981 cagagctcgc cgggaacatc aacggcctga ctgaacgtca cgacctgacc agtgtcataa
     2041 aagaacggag ggaactgctt tggagtcact taaaatagcc tttttctgct gggaatcgat
     2101 gtacgctgaa cgcgtcggcg ggcttgccag tgctgcgacg catctcgcag aaacactggt
     2161 aaaccagaac catgaagtgc atttcttcac gcggggaaat atttctgatc aggttattaa
     2221 aggtgttcat tatcactatt gccagccctc cggaaaaaac atagtcgaat tttgcgacag
     2281 catgagcgcc tcgatggtgg accagtttta ccgctatgat agaccccaca ggtttgacgt
     2341 tcttcatttc catgactggc atccggtcca ggctctccat cacctgcagg acaggaatac
     2401 aatcctgagc tttcattcaa cagaatacgg gaggtttggc aaccagttcg gcgactggtg
     2461 ggaatttaag gaaatttccg ggaaagaatg gtatggcggg cttattgcca aacgggtaac
     2521 cgcagtatcc gaaaccctga aaaaagaact gatgcagtta tacaatatcc ctgacgaaaa
     2581 atgtgatgtg attccaaacg gtgtgatccc ccgcgagttc cggtcagcga tcgatccggg
     2641 ggaagtgaag aaatcgtatg gtatacaccc ctacgcaccg ctcatcctgt ttatcggcag
     2701 gcttgtctac cagaaaggtc cggacctgct cgtcgaggcg atacggaaag tctgccagca
     2761 ccgctgggac gcacaggtcg ttgttgccgg ggaagggggc atgcggcagt tcctggagga
     2821 gcgggcaaaa gatctgccgg tcaactttgt cgggtacatc ccggattctg agtatgtccg
     2881 gttattgaac gcaactgata tcgtcgtaat acccagccgg aatgaaccat tcgggcttgt
     2941 actcctggag gcatggagtg ccgaaaaatg tgttgttgca tccaatgtcg gcggactctc
     3001 tgagaacatt gatgcatttg aaaatggtat caaagtggat gttgaccctg aatccctcgc
     3061 atggggcatc agcatgatga tagacgaacc ggagaacgcg ggagccatgg ggaaacgtgg
     3121 cagggccaaa gtggatcgcc tcttcctctg ggatccgatc gtcaggggac tgacagctac
     3181 ctatgcccgt acggttgcat gaaaatatgt atgtcgctga agtgttccct tacgttattg
     3241 aggggcctga acacgggtat tgtctcccat cggccccgta tatgcatcat gaaggtccgg
     3301 tgtatgcctc aggtcaggag gagaaataca ggggacctga ttcatgactt taaaggaatt
     3361 atccatgcat cttaaaaact ttaaagacac cattgcagct tcgccctgta tcttcactct
     3421 tggtgttgcc ggggacagcg ggtcgggaaa aaccacgttc acccagggta tacggagcat
     3481 ctttggtgat gatctcgtgt caactattac acttgacgat tatcacagcc ttgatcgggc
     3541 cgggcgaacg gaacagggaa taacggcgct ggacccccgg gcaaacagga ttgagcagct
     3601 cgaacgggac ctcatcttgc tcaaacaggg gatgcagatc gaaaaaccgg tctacaacca
     3661 tgctaccgga acatttgatc cacccgtcct cttccggccc aagaaaatcc tgatccttga
     3721 agggctccat acgctgttca ctccgacgct gcgaaaatgc cttgatttta ccctctttgt
     3781 ggatccggca cagcaggtga aatatgactg gaagatccgc cgcgacatgg aaaaacgggg
     3841 ttattcccat gacgccgtta tgaaagaaat tactgaacgt gaaccggatt accagcggta
     3901 tattgccccg cagaaggaat tcgcggatgc cgtgatcggg atcgactatt cggaattcgg
     3961 gcaggacctg ggactggagc gcaatgtcta ccggatcacc ctctcgcaga accggatgaa
     4021 ccacgccatg gaagatatcg atctttcact ggatctctat tccatcctct ccctttccca
     4081 gcggaatttc tctcttaaat tcctgaccgg tcatcataat aaccaccgga taggtcagtt
     4141 ggtcatggat ggcgaactcc gggaagatgt tggggaaaaa cttgaacgca gcatcgagaa
     4201 ccagacacgg gtccacccca tctcactctt cagacaacgg gactacgtac ctgccgggga
     4261 tcttgcccag ctgattctct gctggcggat tattcaccgc cgcattttca tggagacctc
     4321 ccggtaa
//
LOCUS       DASRLV010000192        12401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_635189,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000192 DASRLV010000000
VERSION     DASRLV010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 12401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12401
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635189"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VFC43_07295"
     CDS             <1..302
                     /locus_tag="VFC43_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HZK31017.1"
                     /translation="FKEAVRCLAAADPKTLVINRRISRLTYAHRCLEGAAVQAYRDRG
                     IEIAPGMKIGYVVRDARTYSVDTEWDAAVFDLPYYHNLLEKAWQEIAFAFRTIPS"
     gene            407..1621
                     /locus_tag="VFC43_07300"
     CDS             407..1621
                     /locus_tag="VFC43_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HZK31018.1"
                     /translation="MMFSPGSDEIYKESLALHEKHRGKLEIHAKVPLKNTHDLSLAYT
                     PGVAEVCREIAKDKNLAYKYTLKANTIAIVTDGSAVLGLGNIGGYAAIPVMEGKAILF
                     KEFAGIDAFPICFENYDTDFTDQVKNIAPVFGGINLEDIAAPKCFEVEDALQDIGIPV
                     MHDDQHGTAVVVLAALLNSCKVTGKKYEDLKIVICGAGAAGYAITRLLKCIGYDPNVC
                     ATVHEIIVCDTKGTIFRGREGLYKNKYKFIIAEETNRKALAGTLADALKGADVFIGVS
                     GPEIVTEEMILSMNKDPIVFALANPVPEIWPDAAKNGGAAVVGTGRSDFPNQINNVLA
                     FPGIFRGALDARATRITDEMKIAAAHAIAGYIKKPKNDQILPNILDKNVTRAVAAAVA
                     KAAENCGCSRKI"
     gene            complement(1628..1930)
                     /locus_tag="VFC43_07305"
     CDS             complement(1628..1930)
                     /locus_tag="VFC43_07305"
                     /inference="COORDINATES: protein motif:HMM:NF022420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2769 domain-containing protein"
                     /protein_id="HZK31019.1"
                     /translation="MDAFEQWLNMSWEKNAKERKQITLEKREICLCPECPSYNRCARE
                     HDELLYCITGKSMLCISEDTGCTCKKCPVTRDLGLMYHDFCLKGGEATQRYEHEVH"
     gene            complement(1958..2350)
                     /locus_tag="VFC43_07310"
     CDS             complement(1958..2350)
                     /locus_tag="VFC43_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZK31020.1"
                     /translation="MADEPLIEADDLSWEKTVERGTRPVAVMFYSPTCAFCHQMEPYF
                     RSYAGEYKDSVVFVRINIMANQWTAERYGVLSTPTFKFFCEGKPVQEMVGAIYPSLLK
                     KMIDEVLVHGKECAKNSTTINYDITGYG"
     gene            2508..3938
                     /locus_tag="VFC43_07315"
     CDS             2508..3938
                     /locus_tag="VFC43_07315"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZK31021.1"
                     /translation="MLMRIGGSDVEGVDSRWIDVKNPATGELIDKVPAGTDEDVSRAV
                     DSAEAAFAAWAKKAMRDRGMILYHAAQKVRDQHKDLARLLTLEQGKPIRESIDEVRGY
                     ANILEFYAGISAHQQGEAIRLAHQGDCIVVHQPIGVCAAIIPWNMPVLIMGWKIGPAL
                     LAGNTMVLKPASSTPLTNLRLAQILEEAGLPAGVLNIVTGRGESAGEALVKHPAIRKI
                     SFTGDCTTGGKIRQIAGMHKKDVTLELGGSDPMIVMHDAAIDKAVEGALRGRFYNAGQ
                     TCTAVKRLYVHEKIATVFTQKLKERVDTLSVGNGLGSRIDMGPMISKEQREKILQAVE
                     ETRQKEEGTILSGGSTLKGSAYEQGYFYRPTLVSNVVPGARLVTNEIFGPVLPVMTVP
                     DLDTAIREANTTQYGLGASVWTQDLATVKRVFDEVKAGIIWVNRHLTVPPEVPFGGVK
                     ASGNGRENGQHALDGYTQTRTLFLGW"
     gene            complement(3941..4963)
                     /locus_tag="VFC43_07320"
     CDS             complement(3941..4963)
                     /locus_tag="VFC43_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31022.1"
                     /translation="MTNPFVRALVCLLVLCAIIIIPPASSVTLSPGNSFAGAPTIANG
                     DPVFIDGIATGHPANGLQVWLLGYNYVKISSVYTNTDNTYEFELKSADTQNLAPGQYY
                     VLVQHPMMNGEFDIFIDASKGSVINRQLGTGTSIFQLTGTGSLQTPDGANALMRAINS
                     QNLDDTFATTSFIIRNPTAFIDPIGDHAVGDKFTITGSTNLAAGNNLLVEITSSSFRP
                     SQKSQSGEFSGSSGTVTIDPGSGGMNRWSFDVDASTFRPDEYLVKISGTVIEVAGSTT
                     FNIFEKLPATVKTPVPSTSVPAVSLTQTTPPPSPVSTTQKSPTSPLGIIAAIAVVAGF
                     LRTKRK"
     gene            complement(5130..5471)
                     /gene="rnpB"
                     /locus_tag="VFC43_07325"
     ncRNA           complement(5130..5471)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFC43_07325"
                     /product="RNase P RNA component"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00373"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="archaeal type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00373"
     gene            5809..6372
                     /locus_tag="VFC43_07330"
     CDS             5809..6372
                     /locus_tag="VFC43_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00296 family protein"
                     /protein_id="HZK31023.1"
                     /translation="MYLLDAKEGDMAVQLARGAIEYTIAKKPKPPLLLTPIFSDKRGV
                     FVTLTKAGELRGCIGFPYPVMPLGDAIEDAAVAAATGDPRFPTVRKDELASVRLEVTI
                     LTVPFPVEGDPAKRQEKIEVGRHGLIVRGRGTSGLLLPQVATEYGWDSVTFLEHTCTK
                     AGLPERCWTQANIEVLTFEGQIFSEHR"
     gene            6426..7880
                     /gene="tgtA"
                     /locus_tag="VFC43_07335"
     CDS             6426..7880
                     /gene="tgtA"
                     /locus_tag="VFC43_07335"
                     /EC_number="2.4.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(15) transglycosylase TgtA"
                     /protein_id="HZK31024.1"
                     /translation="MAIAFESLYSDIAGRCGKLTVGKKTIKTPALLPVINPHLQLVTP
                     KEIQSMGVEALITNAYIFSQSQQYRERALTEGLHRVLDFDGVIMTDSGSFQLSVYGQV
                     SITNTETLSFQRDIGSDIWVPLDIPTSPGSDRDTTERELGITLQRLAEAQRVFGRDAP
                     IAGPVQGGSFTDLRKRAGREVTNLGFSFCPIGAVVPLMESYRYRDLVSVVMAAKQTLS
                     PSACVHLFGAGHPSMFALATAMGCDLFDSAAYALYAKEGRYLTTHGSFKIDELADLPC
                     ACNICRTHTCEELRTAAGRDRLLAFHNLYVTLAEISRIRQAITDGTLWELVDERCRGH
                     PQLLAGYRALLNTTASLEKTDRVSKRRFFYRGDESCRRTEVFRYQQMLPRLVLGKKVL
                     IAFEGGTRDEFTDTLFFKPPFGPYPKELKETFPIGPSELPDWDDTMVRTGCRGIRLLM
                     ESHPDSQFVVSCNTRWEGIVRQELGEIQVRNDTV"
     gene            7867..9498
                     /gene="arcS"
                     /locus_tag="VFC43_07340"
     CDS             7867..9498
                     /gene="arcS"
                     /locus_tag="VFC43_07340"
                     /EC_number="2.6.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002948 - archaeosine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0002927 - archaeosine-tRNA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosine synthase subunit alpha"
                     /protein_id="HZK31025.1"
                     /translation="MTRFNIRKRDGLARTGIFETGSTSVKLPFALAAETLFPSLETLM
                     GTNLPLCAPAALVNRFPPETGSQPVTIHPLRDNPAQSGDCVMVANWHTALLNPRNYTD
                     WLIGLKEKTPVDTAWYAPGTALPSNAHILCYSGFDLFDFLAVDLKTAQALFCTPEGEF
                     PAGLLGSGICTCAGCQTKNLLQHNRQALLREIALISRFIEASQLRDLVESRCRMNAAH
                     VAIMRHLDNQYPFTERHLPIARSGVMRANSGESLQRAEVRRFAERVINRYCPPKTDVA
                     VLLPCSAKKPYSLSQSHKKFQRAIAGRAHELIVTSPLGLVPRELECIYPAGHYDVPVT
                     GYWDAEECSVIARILARYFKRHAYRRIIAHLEGGALKVAQMAAGKCGITLEFTCKERP
                     TAESALNALDAALAGERRMKDDRLHGMASYQFSIDLETKGTTVRGHYPDLFYSKNNVQ
                     IFSIDTGTGMLRPTFDGWHLIPEGYRVYIDDFIPEGDVLVPGVVRADPGIRDGDEVLV
                     VGPRALATGRAALPTEDILRSRRGVAVRVRKIKRL"
     gene            9550..10017
                     /locus_tag="VFC43_07345"
     CDS             9550..10017
                     /locus_tag="VFC43_07345"
                     /inference="COORDINATES: protein motif:HMM:NF017072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31026.1"
                     /translation="MFVTGKAENTISPATNAPIHNPIVIAVPKELATGEQRVAIVPEI
                     VQNLKKSGHEIRIEHDAGTSAYYPDALYTAAGAKIVSIDDLFNGARIVLRVQPPTLAE
                     VDQLAEGTIVIGFMNATNNLEVIARMRDRRITAFALELVPRITRAQSMDALSS"
     gene            10045..10749
                     /locus_tag="VFC43_07350"
     CDS             10045..10749
                     /locus_tag="VFC43_07350"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31027.1"
                     /translation="MLGADNCPKFLPMLTTAAGTIRPATVLILGAGVAGLMAIATAKR
                     LGAIVEAYDVRRAAGEQVRSLGAKFLELEINAEGQGGYARELTPEEKEKEQLMVSTAV
                     ARADIVITTAAIPGRKAPRLVTKETVASMRPGAVIVDLAAESGGNCELTVAGKTVREY
                     GVTIIGPQNLPARVPFHASQMYAKNLQSFLALLVDKNGALISDFTDEILVASLLVHAG
                     EVRHKPTSDLMSGGKP"
     gene            10746..11057
                     /locus_tag="VFC43_07355"
     CDS             10746..11057
                     /locus_tag="VFC43_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HZK31028.1"
                     /translation="MTDVTTLLTAVYIAMLAAFTGYVVISRVPAILHTPLMSGSNFIH
                     GIVLVGAMVALGLATTPLEQIIGFIAVVLGAANVTGGYVVTERILQMFDRSGKKPGKE
                     D"
     gene            11061..>12401
                     /locus_tag="VFC43_07360"
     CDS             11061..>12401
                     /locus_tag="VFC43_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HZK31029.1"
                     /translation="MIDLSWAIDPIYIATIFFFIVGMQRMSHPLTARSGIVWAGAAML
                     IATLITFFTPNLTNFALIAIAIVIGGGFGYIAAKRVGMTDMPQMIALYNGMGGGAAAG
                     ISAVALLSTTNAITSTLAIVGGLIGAVSFTGSIIAFLKLQGWMRPRPITFPGQQIVNM
                     VVLALAIVSGVLVIVPPAWIPLSIAVYLPLFFLLSLGFGLLMTLPIGGADMPVVISMY
                     NAFTGLAVALDGFSFVTPNYAMVIAGIIVGAAGSLLTLNMAKAMNRSLSNVFFGAFGA
                     TEEKGLEIQGSMKSIEVEDVAVLLAYADKVIVAPGYGMAVAQAQQKVKELTDLLEAKN
                     VQVKFAIHPVAGRMPGHMNVLLAEAGVSYDHLFDRDEVNPEFPSTDVVLVIGANDVVN
                     PAAHRHGSPLFGMPILDVEQAKNVVVLKRGQGKGFAGIENDLFYRDNTRMLYGDA"
BASE COUNT         2924 a         3175 c         3374 g         2928 t
ORIGIN      
        1 ttttcaaaga ggcggtacgg tgtcttgccg ctgcagaccc aaaaacactg gtgatcaatc
       61 gtcggatcag ccggcttacc tatgcacacc gctgtcttga aggagccgca gtgcaggcat
      121 accgcgatcg tggcattgaa attgcaccgg gaatgaagat cgggtatgtt gtccgcgatg
      181 cacggacata ctctgtggat accgagtggg atgctgctgt tttcgatctc ccttactacc
      241 ataacctgct ggaaaaagcg tggcaggaaa tagcgtttgc attccggaca attccctcat
      301 aaaaagtgca cgttgggggg aatggcgtgc tgcccgcaga agcaactggc tgccagcaac
      361 attcattgcc ttgacgcaca atctcacgta taaggaatga tgtggaatga tgttttcacc
      421 gggaagcgac gagatctata aggaatctct tgccctgcac gagaaacacc gggggaaact
      481 ggagatccac gcaaaggtcc cgttaaaaaa cacccatgat ctctcccttg catacacccc
      541 gggtgtcgct gaagtgtgtc gggaaattgc caaagataag aatcttgcat acaagtatac
      601 cctgaaagcg aacacgattg ctatcgttac tgacggctct gcagtcctcg ggcttgggaa
      661 catcggagga tatgcagcca tacccgtgat ggagggaaaa gcaatccttt ttaaggagtt
      721 tgccggcatc gatgctttcc cgatctgttt tgaaaattat gatacagatt ttactgacca
      781 ggtcaagaac attgcaccgg tattcggcgg catcaatctc gaagatattg cagcacccaa
      841 atgttttgag gttgaagacg ccctccagga tatcgggatt cccgtgatgc atgatgacca
      901 gcatgggact gcggttgtgg ttcttgcagc acttctcaat tcctgtaaag taaccgggaa
      961 aaagtatgag gatctgaaga tagttatttg cggagccggt gctgccggct atgcgatcac
     1021 caggttactc aaatgcatcg gatatgatcc caatgtctgt gccaccgtgc acgagattat
     1081 tgtctgtgat acgaagggga ctatcttccg tggaagggaa ggattgtaca aaaacaagta
     1141 caaattcatt attgcagagg aaacaaaccg caaggctctt gcgggaactc tcgcggatgc
     1201 attgaaagga gcagatgtct ttatcggtgt ttctggtccg gaaattgtca cagaagaaat
     1261 gatcctgtcg atgaacaagg atccgatcgt atttgcactg gcaaacccgg tgcccgagat
     1321 ctggccggat gcagcgaaaa atggcggggc agcagttgtc ggcaccgggc ggagtgattt
     1381 tcctaaccag atcaataatg tgcttgcatt cccgggcatt ttccgggggg ctctcgatgc
     1441 ccgtgccaca cggatcactg acgagatgaa gatcgctgct gcgcacgcaa ttgcagggta
     1501 tatcaaaaaa cccaaaaatg accagatcct gcctaatatt ctcgataaaa atgtgacgcg
     1561 tgcggtagcg gcagcggtgg caaaggctgc cgagaattgt ggttgcagca gaaaaattta
     1621 atttcgttta atgcacttcg tgctcgtagc gttgagttgc ttctccgcct ttcaggcaga
     1681 aatcatgata catgagcccg agatcccggg taaccggaca ttttttgcag gtacatcccg
     1741 tgtcttctga aatacagagc atactttttc cggtaatgca atacagcaac tcatcgtgtt
     1801 cccgggcgca gcggttgtag gaagggcatt ctgggcagag acagatctcc cgcttttcaa
     1861 gagtaatctg cttacgttct tttgcatttt tctcccagga catgttcagc cattgttcaa
     1921 atgcatccat acaaatatct ccaattctcc cgccagctta tccatagccg gtgatatcat
     1981 aatttattgt tgtcgaattc tttgcgcatt cttttccgtg gacaagtact tcatcgatca
     2041 tttttttgag cagtgaagga tagatcgccc ccaccatttc ctgcacaggt tttccctcac
     2101 agaaaaattt gaaagtaggg gtactaagga caccatagcg ttcagcagtc cactggtttg
     2161 ccatgatgtt tatccgcaca aatactacgg aatctttgta ctcacctgca taactcctga
     2221 aatagggttc catctggtgg cagaatgcac acgtgggact gtagaacatc actgcaacgg
     2281 gccgggtgcc tctctctacg gttttttccc agctgaggtc gtctgcttca atcagcggtt
     2341 cgtctgccat caggttccct cttagccaga actgagagga tgctgctcat atagtttgtc
     2401 ctgccgtgga gttttaccga tgatgcagat cgtttattgc agtcttcccg ccagtttatt
     2461 gcataaggaa cgcatatttc cctcaggaat acaggagtga gaagtcaatg ctgatgcgga
     2521 taggaggaag tgacgtcgaa ggagtggatt cccgctggat tgacgtgaag aatcctgcaa
     2581 ctggcgaact gatcgataag gtgcctgcag gaacggacga ggatgtatcc cgggcagttg
     2641 attccgcgga ggcggctttt gctgcctggg caaaaaaagc catgcgtgac cgcgggatga
     2701 tcctctatca tgcagcacaa aaggttagag accagcacaa ggatcttgcc cggctcctta
     2761 cccttgaaca gggcaaaccg atacgggaat ctattgatga agtgcgggga tatgccaata
     2821 tccttgaatt ctatgcgggt atttccgcac accagcaggg tgaagctatc cggctcgcgc
     2881 accagggtga ttgtatcgtt gtacaccagc caatcggggt ctgcgctgct atcattccct
     2941 ggaacatgcc ggtactcata atgggctgga aaattggccc tgccctgctt gcaggaaaca
     3001 cgatggttct taagccggca tcatccactc ctctgacaaa cctgcggctg gcacagatcc
     3061 ttgaagaggc agggcttccc gcaggggtcc tcaatatagt gaccgggagg ggcgagtcgg
     3121 ccggtgaggc tctggtgaag cacccggcaa tccggaaaat atcatttacc ggtgactgca
     3181 cgacgggcgg gaaaattcgc cagattgcag gaatgcacaa aaaggatgtc acgcttgagc
     3241 ttggggggtc tgacccgatg attgtcatgc atgatgcagc aatcgataag gcagttgagg
     3301 gagcactccg gggcaggttt tacaatgcag ggcagacctg cactgcagtc aagcggctgt
     3361 atgtccatga aaaaattgct acggttttta ctcagaaact gaaggaacgc gtagataccc
     3421 tgtcagttgg aaacggactg gggtccagaa tcgatatggg tccgatgatc agtaaagagc
     3481 agcgcgagaa aatcctgcag gcagttgaag aaacacggca gaaagaggag ggcacaatcc
     3541 tctccggagg atccacatta aagggatcgg cctatgagca gggctatttc tatcgcccga
     3601 cactggtcag caatgtggta ccgggtgcgc gactggtgac aaacgaaatt tttggccctg
     3661 ttctcccggt aatgaccgta ccggatcttg ataccgctat ccgcgaggca aatactacgc
     3721 aatacgggtt gggagcttct gtctggactc aggaccttgc gacggttaaa cgggtatttg
     3781 atgaagtaaa agccggtatc atctgggtga accgtcacct tactgtgccg cccgaagtcc
     3841 cattcggggg tgtaaaggcc agtggaaatg gacgggagaa cggccagcat gctctcgacg
     3901 gctatacgca gaccaggacc ttgtttctgg gctggtaaaa ttatttcctt tttgttcgca
     3961 gaaaacctgc gaccacagcg attgctgcga ttattccaag gggtgaagtc ggggactttt
     4021 gagtcgttga aacgggtgaa ggtggcgggg tggtctgagt gagcgatact gccggaacgg
     4081 aggttgatgg tacgggtgtt tttaccgtcg caggaagttt ttcaaatatg ttgaatgtgg
     4141 tagatcctgc tacctcaatg acggtccctg atatcttgac aagatattca tccggcctga
     4201 atgttgacgc atcgacgtca aatgaccaac ggttcattcc cccggagccc ggatctatcg
     4261 tgactgttcc ggatgaaccg gaaaattcac cgctctgcga tttctgcgat ggtctgaacg
     4321 atgacgaagt gatttcaacg agaagattat taccggctgc aaggttggtc gaaccggtga
     4381 tggtaaactt gtcaccgaca gcatgatccc cgattggatc gataaatgca gtgggatttc
     4441 tgatgataaa tgacgtggtg gcaaatgtat cgtcaaggtt ctggctgttg atagcccgca
     4501 tcagcgcgtt ggcaccatcg ggagtctgga gactgccagt accggtgagc tggaagatgg
     4561 aagtaccggt tcccagctgc cggttgatca cactcccctt cgaggcgtca ataaaaatat
     4621 cgaactctcc attcatcatc gggtgctgga caaggacata atactggccg ggtgcaaggt
     4681 tttgcgtatc ggcggattta agttcgaact cgtatgtgtt atccgtattt gtgtaaaccg
     4741 aggaaatctt tacatagtta tatccgagca gccagacctg taaaccatta gccgggtgac
     4801 cggttgcaat accatcgata aataccgggt ccccgtttgc gattgttggt gctccggcaa
     4861 aggagttgcc cggagataag gtgacggacg atgccggtgg gataataatg atagcgcaca
     4921 ggaccagaag gcaaaccaga gcgcgaacaa aggggttcgt catattaatc agctgatatg
     4981 gtgcctgaac gctgatgaat gttgtctggc ggatatctgt cagaatctgt tcaggttaaa
     5041 aaccatggat ctggctgcga tggttataaa acagttttac ggtgcagata cgctgccgac
     5101 cccacatgag gcaggccgta ataaagaatg agtgggagga gtaagcctcc ttctgtttca
     5161 gcggcattca gctctgcgtc atacccggac ggataccgag ctatccattg tcctgttctg
     5221 acttgcaccc gcaggggttc gccgtttcat cgtttcctgt ttgtagacgc tcagcgagtt
     5281 atcacacgtt tatgtgtgca ctcgacggta ttaccgtctc gatcgcttca taactgaaaa
     5341 caggccgtgt cgtttctgtg ccattaccgt gactctcgcc acccgtactt gtgtacggct
     5401 gcgtgcccgg tgggtgggag gactttcctc agcagccaga aatggccacc gtcggccgct
     5461 ctctccctct cctttactac gttgattttt caaagagata agcacagcgc ctatgagcgg
     5521 agttgccttt tatgaaatac agaatccgcg cttactgaag atgataaaca atttctgcga
     5581 tagtacggat attttatgtt atttttttct ttataactcg ctggtaaatc tctaaaaaga
     5641 gccattgcat ctgacctgtc atgggaaatc aggacggtat agtgtaaccc cgccgtggtc
     5701 atgcgtggtt ttatttcgca aaaacaggaa atctcccaaa aaacctgttc gatagattgt
     5761 taaataaggt gcactaaaga cgttcacaag ggtaaggggg aaatgtgaat gtacctgctc
     5821 gatgcaaaag aaggagatat ggcagttcag ctggcacgcg gtgccattga atatactatt
     5881 gcaaaaaaac caaaacctcc actgttactg acacccattt tttcggataa gcggggagtt
     5941 tttgtcacgc ttaccaaagc cggagaactt cggggctgta tcggttttcc gtacccggtg
     6001 atgccccttg gagatgccat agaggatgcc gctgttgcag ctgcaaccgg agacccccgg
     6061 tttcccactg tccgcaagga cgaacttgca tctgtccggc tcgaagtcac gatactgact
     6121 gtccctttcc ccgttgaggg agaccctgca aaacgccagg aaaaaattga agtgggcagg
     6181 catggcctga tcgtgcgtgg caggggaaca agcgggcttc tgcttcccca ggtggccacc
     6241 gaatatggct gggacagcgt gacatttctt gaacatacct gtactaaagc agggcttccg
     6301 gaacgatgct ggactcaggc aaatatcgag gtgctcacgt tcgaagggca gatcttcagc
     6361 gagcacaggt gagggcaacc gtttaacctc aataccgcac atataagggc acaacacggt
     6421 gcacaatggc cattgctttt gagagtttgt acagcgacat cgcgggacgc tgcggcaaac
     6481 tgacggtagg gaaaaagacg atcaagaccc ccgcgctcct gcccgtcatc aacccccacc
     6541 tccagctcgt aacgccaaag gagatccagt ctatgggcgt tgaggcactc atcacaaatg
     6601 cctacatctt ttcccagagt caacagtaca gggaacgcgc cctgacagag ggactgcaca
     6661 gggtgctgga ttttgatggt gttattatga ccgattcggg ctcgttccaa ctctcggtct
     6721 atggtcaggt ctccatcacc aataccgaaa cgctctcctt ccagcgtgat atcgggagtg
     6781 atatctgggt tcccctggac atcccgacat ccccgggatc cgaccgtgac accactgagc
     6841 gggaacttgg cataaccctg cagcggctcg ccgaggcaca acgagtcttt ggccgggatg
     6901 caccaatagc aggaccggtc cagggcggca gctttactga tctccgaaaa cgggcgggcc
     6961 gtgaagtaac aaatcttggt ttttccttct gtccgatcgg cgccgtggtc cccctgatgg
     7021 aatcctaccg gtaccgggat cttgtcagcg ttgtaatggc tgcaaaacag acactttcac
     7081 cttctgcatg cgtccacctg ttcggggctg gccacccttc aatgtttgcc ctcgccacag
     7141 caatgggatg cgaccttttt gattctgctg cgtatgcact ttatgcaaaa gagggacgtt
     7201 acttaaccac gcatggcagt ttcaaaatcg atgagttggc ggatctcccc tgtgcatgta
     7261 atatatgccg gacacatacc tgtgaagaat tacgtactgc tgccggtcgt gaccgcctgc
     7321 ttgccttcca caacctgtac gttactcttg ccgaaatttc ccggatccgc caggcaatca
     7381 ccgatgggac gctctgggaa ctggttgatg aacggtgccg ggggcatcca cagctccttg
     7441 caggttaccg ggcgctcctg aatactacag catccctgga aaaaacagac cgggtatcaa
     7501 aacggcggtt tttttaccgt ggagatgaga gctgccgtcg cacggaagtg ttccgctacc
     7561 agcagatgct cccgcggctg gtgctgggaa aaaaagtcct tattgcattt gagggtggta
     7621 caagggatga attcaccgac accctgttct tcaaaccgcc gtttggtccg tatccaaaag
     7681 aactcaaaga gacgtttccc atcgggccga gtgaactgcc cgattgggac gatactatgg
     7741 taagaacagg atgcagggga atacgattgt tgatggagag ccatccggac tctcagtttg
     7801 ttgtttcctg caatacccgc tgggaaggaa ttgtccgtca ggaacttggc gagatacagg
     7861 tgcgcaatga cacggtttaa tataaggaag cgggacggtc ttgcgcggac cggtatattt
     7921 gagaccggaa gtacttcggt taaattgccg tttgctttag cagcagaaac gctttttccc
     7981 agccttgaaa ccctcatggg aacaaacctg ccgctttgcg cacccgctgc tctggtgaac
     8041 cggttccctc ctgagactgg cagccagccg gttacaatcc accctttgcg tgataatcct
     8101 gcacagagtg gcgattgcgt gatggttgcc aactggcata ccgcattatt aaatccaagg
     8161 aattatactg actggcttat cggccttaaa gaaaaaacac ctgttgatac cgcatggtac
     8221 gcgccaggca ctgcattacc ctcaaatgct catatcctgt gttattcagg gtttgacctg
     8281 tttgattttc tggcagtcga cctcaaaacc gctcaggcgc tcttttgtac acccgaagga
     8341 gagttccctg ccggtttgct tgggagtggg atctgcacct gtgcaggatg ccagacaaag
     8401 aacctgctcc agcacaaccg gcaggcactt ctccgtgaga ttgcacttat ttcaaggttc
     8461 attgaagcct cacaactccg cgatctggtg gaatcacggt gccgcatgaa tgcagcccac
     8521 gttgcgatca tgcgacatct tgacaaccag taccccttca cggaacggca cctgcctatc
     8581 gcccgaagcg gcgttatgcg ggcaaattcg ggagaatccc tgcagcgggc tgaagtacgt
     8641 cgttttgccg aacgggtgat caacaggtac tgtccgccaa aaactgatgt tgccgtcctg
     8701 cttccctgtt cagccaaaaa accctattcc ctctcccaga gtcataaaaa attccagcgg
     8761 gcaattgccg gtcgcgccca cgaacttatc gtcacttctc cgctgggact ggttccccgc
     8821 gaacttgagt gcatctaccc tgctggtcat tatgatgttc cggttaccgg ctactgggat
     8881 gcagaagagt gttctgttat cgcacgtatc ctcgcgcgct atttcaaacg gcatgcatac
     8941 cgtcgtatta tcgcgcatct tgaaggggga gcactgaagg tggcccagat ggcagccgga
     9001 aaatgcggta ttaccctgga atttacctgc aaggaacgtc ctaccgccga atcggccctg
     9061 aatgcacttg acgctgccct tgcaggagaa cggcggatga aagatgaccg gctccatggc
     9121 atggcatcat accagtttag catcgatctt gaaacaaaag gcacaacggt acgcgggcat
     9181 tacccagacc ttttttacag taaaaataat gtccagatct tctcgataga taccggcaca
     9241 gggatgcttc gtcccacgtt tgacggctgg cacctgatcc cggaaggata ccgggtgtat
     9301 atcgatgatt tcataccgga gggggatgtc ctggtaccag gtgtcgtgag ggcagatccc
     9361 ggtatacggg atggtgatga agtgctggtt gtcggacccc gtgcactggc caccgggcgt
     9421 gcggctctcc caactgagga catcctccgt tcgcggcgcg gtgtggcagt acgggtgcgt
     9481 aagataaaga gactgtaagg tacatgtata cgatacattg cttacgcatc aatgtgagga
     9541 gtgacagggt tgtttgttac agggaaagca gaaaatacaa tatcgccggc aacgaacgca
     9601 ccgatacata atccaatcgt tatcgccgtt ccaaaggagc tggccacggg ggaacaacgc
     9661 gtggctatcg tgccggagat tgttcagaat ctcaaaaagt cgggtcacga aatccggatc
     9721 gaacacgatg cggggacgag cgcttactac cccgatgctc tctatacagc cgccggtgcg
     9781 aaaatcgtgt ccattgatga tttgttcaat ggtgcccgga tcgtgctgcg cgtccagcct
     9841 ccaactctcg ccgaggttga tcaactcgct gaaggtacga ttgtcattgg attcatgaat
     9901 gcaacgaaca acctcgaggt tatcgcccgg atgcgggatc gcaggatcac tgcctttgcc
     9961 ctggaactcg tcccgcggat tacccgagcc cagagtatgg atgcgctcag ttcataggca
    10021 acagctgccg gatacactgc agcaatcctt ggtgcagaca actgtccaaa gtttctcccg
    10081 atgctgacga ctgccgcggg gactatacga ccggcgacgg tgcttatcct gggtgccggc
    10141 gttgccggcc ttatggcaat cgctactgca aagcggctcg gcgcaattgt cgaggcctac
    10201 gatgtccggc gggcagccgg ggagcaggtc cggagccttg gcgcaaagtt cctcgaactt
    10261 gagatcaatg ccgaaggcca gggaggttac gcccgcgagc tgacacccga agaaaaggaa
    10321 aaagaacaac tgatggtatc aaccgcagtc gcccgggccg acattgtcat cacaacggct
    10381 gcgatccccg gtcgtaaggc cccccgcctt gtcactaagg aaaccgttgc atctatgcgg
    10441 ccgggtgctg tcatcgtcga ccttgctgcc gaatctggcg gtaactgtga gctgaccgtg
    10501 gctggtaaga ctgtcaggga gtacggggtt acaattatag gtccgcagaa ccttccggcg
    10561 cgggtgccgt tccatgcgag ccagatgtac gcaaagaatc tccagtcatt ccttgccctt
    10621 cttgtggata agaatggagc gcttatcagc gatttcacgg acgagatcct ggttgcgagc
    10681 cttctggtac atgcaggcga ggtgcgtcat aagccgacca gcgatctcat gagcgggggg
    10741 aaaccatgac ggacgtaact acgctcttga ccgcggttta catcgcgatg ctggccgcat
    10801 tcaccggtta tgtcgtcatc agccgggttc ctgccatcct ccacacgccg ctgatgagcg
    10861 gttcgaactt tatacacggg atcgtgcttg tcggcgccat ggttgcgctc ggcctcgcga
    10921 cgacacctct cgaacagatc atcggcttta ttgcagttgt cctgggcgca gcgaacgtaa
    10981 ccggcggcta tgttgtcacg gagcggatcc tccagatgtt cgaccgcagc gggaagaagc
    11041 ccggtaagga ggactaaaag atgatagacc tttcctgggc aatcgacccg atctatatcg
    11101 cgacgatctt cttcttcatt gtcggtatgc agcggatgag ccacccgctc actgcccgaa
    11161 gtggaattgt ctgggccggt gctgcgatgt tgatcgcgac gcttatcacg ttcttcactc
    11221 caaaccttac caacttcgcc ctgatagcaa tcgcgatcgt cattggtggg ggcttcggct
    11281 acattgcggc aaagcgtgtc gggatgaccg acatgcccca gatgatagcg ctctacaacg
    11341 gtatgggggg cggtgcggca gccgggatct cggcagttgc acttctcagt acaaccaatg
    11401 caatcaccag tacccttgca attgtgggcg gcctcattgg ggcagtctcg ttcacgggga
    11461 gtatcattgc gttcctgaag cttcagggtt ggatgcggcc acggccgatc acctttcccg
    11521 gccagcagat cgtcaacatg gtggtcctgg cccttgctat agtctccggc gtgctcgtta
    11581 ttgtaccgcc ggcctggatt ccgctttcca ttgccgtata tctcccgctg ttcttcctcc
    11641 tctcgctcgg gttcgggctc ctgatgacgc ttccgattgg cggggccgat atgcccgtcg
    11701 taatctcgat gtacaacgcc ttcaccggcc tcgcagttgc gcttgacggg ttctcgtttg
    11761 tcacccccaa ctatgcaatg gtcatcgcgg gtattattgt cggcgcagct gggtcgctgc
    11821 ttacgctcaa tatggcaaag gcaatgaatc gctcgctctc gaatgtcttc ttcggtgcgt
    11881 tcggcgcaac ggaagagaaa ggcctggaga tccagggcag catgaagtcg atcgaggtcg
    11941 aagacgttgc ggtcctgctt gcctacgcgg acaaagtgat cgttgctccc ggctacggta
    12001 tggcggttgc tcaggcccag caaaaggtca aggaactgac cgatctgctc gaggccaaga
    12061 atgtccaggt taaattcgca attcaccctg ttgccgggcg aatgcccggg cacatgaacg
    12121 tcctgcttgc cgaagcgggg gtctcgtacg atcacctctt cgaccgtgac gaagtgaacc
    12181 cggagttccc cagcactgat gttgttctcg tcatcggggc aaacgatgtg gtgaacccgg
    12241 cagcgcaccg gcatggcagc ccactcttcg gtatgccaat cctcgatgtc gaacaggcaa
    12301 agaacgttgt tgtcctcaaa cgcggccagg gaaagggttt cgccggtatt gagaatgacc
    12361 tgttctaccg ggataacacc cggatgctct acggtgacgc c
//
LOCUS       DASRLV010000193         4476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_961009,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000193 DASRLV010000000
VERSION     DASRLV010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961009"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(425..1765)
                     /gene="purB"
                     /locus_tag="VFC43_07365"
     CDS             complement(425..1765)
                     /gene="purB"
                     /locus_tag="VFC43_07365"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HZK31030.1"
                     /translation="MAVHPIEERYGTPAMRAVWSEKTRFSCIVAVEVALAKAEAAHGM
                     IPAAAATEISRLAQNASLERSKAIEREISHDMMAIVKAIAEVSGESGRWVHYGATSND
                     ILDTATGLQISQSLDLIDQKLRLLLGVLLKRSDETKSLVCIGRTHGQHGVPTTYGLRF
                     AIWASEVGRHIERLGQLRPRVVVGQMTGAVGTQAALGPQAMDIQATMMEVLDMGTVDV
                     SNQVISRDRYAEYFMFCANVATTLDKIGIEIRSLQRTEIGEVEEAFGALQVGSSTMPH
                     KRNPIKSEQVCGLARIIRSSVEPALQNNTLWDERDLTNSSCERVLFPEASILTDHCIH
                     LMTNVLEGLVINRIAIRRNLAFLHGINMAESVMIELTRRGMNRQDAHERIRSASMQAL
                     AEGNALADVLGKDAEVLRYCQKADIAALLNPDSYIGTAVKQVERVIEKLSPLCS"
     gene            2066..3070
                     /locus_tag="VFC43_07370"
     CDS             2066..3070
                     /locus_tag="VFC43_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HZK31031.1"
                     /translation="MRYFKGQTILSEVHAIPMPYTVSLITMEENEALSRQYFPKVRYE
                     IKSEIYGCCIKLLCDDHTLRDTWEDNFYSMSQNVRSHGRLFVGNDPSYPPDTVLFDSH
                     AKTAFVFNMRYYGWIKSIALSLAGDILEDEHSIWSVHGACLDIGGKGLCLVGAPGSGK
                     TTQTYGILTNPHTRVISDDWFFFRVYEEDILAYGSEKNFYIQEDLATIWKEFGALVPG
                     RTYDSDRRAVTDVRWVIGKGRILPLTTINTLIVLKRDPADPNVAQSLNPDTAMNLFLE
                     NNYFNPHLLVHNSFKTQVRNRYIGDLVGRTQCYCVNTTGTPEETQKLIRSLAGVEQTL
                     "
     gene            complement(3151..3654)
                     /locus_tag="VFC43_07375"
     CDS             complement(3151..3654)
                     /locus_tag="VFC43_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HZK31032.1"
                     /translation="MCARSDETIGETRFITDRMLVTLSRYLRFIGYNTLSANGFAEGN
                     TKEDTLLLNLAIGEDRILLTRDAELAQRGKERAVLVKSDDVMDQVRQLIDGGLIVRRV
                     MMSRCSLCNTVLREAAAAEIRSADYAPRDKKGFIFFWCDQCGKLYWNGSHGKSISERI
                     GVDPKRI"
     gene            complement(3635..4476)
                     /gene="amrS"
                     /locus_tag="VFC43_07380"
                     /pseudo
     CDS             complement(3635..4476)
                     /gene="amrS"
                     /locus_tag="VFC43_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843878.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
BASE COUNT         1079 a         1112 c         1106 g         1179 t
ORIGIN      
        1 agatccgtat gggacactga aaatcccgag atctttacgt gcggttccat gcctactgtc
       61 tgtgttcatt ttcagagttt ataaatatgc tattttttat cgtatgaatt acggaaactg
      121 tggtgagaga tgtcggttgc atacagaata ggttaaaaaa tttccccaaa taaaccattt
      181 cgttacccaa gggaattccc cgatgaatac ggccttttta ttgagatcat tcgtataacc
      241 ggaacgtcat ctcctccgtg gcgcattcat caataagggg aattcacacc tgtatcaggc
      301 tgttttggga gaattttgat gaaaattggg ttttttgact ttcatcataa taatccccag
      361 caagaagatt atgaaaagga gtattttttg gtcctggata tagtaaaaga aatgatatgg
      421 tggatcagga gcacaagggc gatagttttt cgataacccg ctcaacctgt tttactgcgg
      481 ttccgatata ggaatccggg ttaagaagtg ctgcaatatc ggctttttgg caatagcgaa
      541 gtacttcagc atctttcccg agtacatctg cgagtgcgtt gccttcagca agtgcctgca
      601 tacttgctga ccggatccgt tcatgtgcgt cctggcggtt catgccccgc cgggtgagtt
      661 cgatcatgac cgactcggcc atgttgatcc cgtggagaaa ggcaaggttc cggcggattg
      721 caatccggtt gataacaagc ccttcgagta cattcgtcat gagatgaatg cagtgatccg
      781 taaggattga tgcctcaggg aataatacac gttcgcacga agagtttgtc aggtcccttt
      841 cgtcccagag ggtattgttc tggagcgccg gttctacgct tgagcgtatg atgcgggcaa
      901 gcccgcagac ctgttcggat ttaatcgggt tgcgtttatg cggcatggtg gacgaaccta
      961 cctgaagggc accaaatgcc tcttccactt cgccgatctc agtccgctga agggagcgga
     1021 tctcgatccc gatcttgtca agggttgttg ccacatttgc acagaacata aagtattctg
     1081 cgtaccggtc ccgggagatc acctggttgg aaacgtcaac ggttcccata tccagcactt
     1141 ccatcatcgt tgcctggata tccattgcct gtggcccgag tgctgcctgg gtgcctaccg
     1201 caccggtcat ctgccccaca acaacccggg gacggagttg tccgaggcgt tcaatgtgcc
     1261 gcccgacctc gcttgcccag atcgcaaatc gcagaccgta cgttgttggt accccatgct
     1321 gcccatgggt ccgccctatg cagaccaggg attttgtttc atccgagcgt ttcaggagta
     1381 ctccgagtaa aaggcgcaat ttttgatcga tcagatcgag tgactgggag atctgcagcc
     1441 cggtggcagt atccaggata tcgttgctcg tcgcaccgta atgcacccac cgcccggatt
     1501 ccccgctgac ttccgcgatc gctttcacta tcgccatcat gtcatgactg atctcacgtt
     1561 cgatagcctt cgacctctcc agcgaggcgt tctgtgccag acggctgatt tcagttgctg
     1621 ctgctgccgg gatcatgcca tgagctgctt cggcctttgc aagtgccact tccacggcaa
     1681 caatacagga aaaccgggtc ttctcgctcc atacggcacg cattgccggc gtaccatatc
     1741 gctcctcgat ggggtggaca gccatgagaa cagatctgtt gtttagggat atagtattta
     1801 atgatctaaa aacggctata ccatataacc gcgtctgact ctgttttagg atttcgtcag
     1861 gattctttgt gacatgggtg gtgcattaaa aatatgtttt cctctgattt aaaaaggcta
     1921 aatgaaaacc gcacgtcagc gcattacgag cagatcctcg ctgccgcaaa aatgtacgtg
     1981 aagagaaaaa ccatttttat ccctctgatt agcccattaa ggccggggtg ggaaaaagta
     2041 tttaaaaaaa aatcctgacg aaaccatgag atactttaaa ggacaaacaa tcctttctga
     2101 agttcacgcg atacctatgc cctacacagt ttcactcata accatggaag aaaacgaagc
     2161 cttgtcacgg cagtattttc cgaaagtgcg ctatgagata aaatccgaga tctatggctg
     2221 ctgcattaag cttctctgcg atgaccatac gcttcgggat acctgggaag ataactttta
     2281 ttcgatgtcg caaaatgtcc gttcgcatgg caggcttttt gtgggtaacg acccgtcata
     2341 tccacccgat accgtgctgt ttgattctca tgcaaaaacg gcgtttgttt tcaacatgcg
     2401 gtattacggc tggataaaat ctatcgccct cagtctcgca ggggatattc ttgaagatga
     2461 acatagcatc tggtcagtgc acggagcatg cctggatatc gggggaaaag ggctttgcct
     2521 ggtcggtgca cccggttccg ggaaaaccac gcagacctac ggcatactga ccaacccgca
     2581 tacccgtgtt atttctgatg actggttctt cttccgtgtg tatgaggagg atattcttgc
     2641 gtatggttca gaaaaaaatt tttacataca ggaagacctt gcaaccatct ggaaagagtt
     2701 cggggcactt gtaccgggca ggacctatga ttctgacagg agggcggtta ccgatgtacg
     2761 gtgggtcatt ggaaagggga ggatccttcc tttaacaaca ataaacactc tcattgtcct
     2821 caaacgggat ccggctgatc caaacgttgc gcaatcactg aaccccgata ccgcaatgaa
     2881 cctctttttg gaaaataatt acttcaatcc gcatctcctt gtccataatt ccttcaaaac
     2941 acaggtaaga aacagatata tcggagatct ggttggccgg acacagtgtt attgtgtaaa
     3001 cactaccgga acgccggaag aaacccagaa actgattcgt tcattggccg gggttgaaca
     3061 gaccctgtaa tgcgttattt ttttaaaata tgatcagata ctctctggaa agggtgcatc
     3121 aaaaagaaga ggcagggtat atctttcgtt tcaaatcctt tttgggtcaa ctccaatcct
     3181 ttctgaaata cttttcccgt gagatccatt ccagtagagt ttgccacact ggtcacacca
     3241 gaagaagata aatccctttt tatcccttgg ggcataatct gcggaacgta tttctgcagc
     3301 agcagcttcc ctcagcaccg tgttgcacaa cgaacagcgg ctcatcatca ctctgcgaac
     3361 aatcaacccg ccatcgatca gttgccgtac ctgatccatc acatcatcgg acttcaccag
     3421 gactgcccgc tcctttccgc gctgggcaag ctctgcatcc cgggtaagca ggatccggtc
     3481 ctcaccgatt gcgaggttta ggagcagggt gtcttctttt gtattccctt ccgcaaatcc
     3541 gttggcgctc agggtattat acccaatgaa cctgaggtac cggctgaggg tcacaagcat
     3601 ccggtcggtt atgaaccgtg tttccccgat ggtctcatct gacctggcgc acaatttcga
     3661 tcctctcccc gcaatttttg cattgcgttc cttcgagtgc cctgatcgtg ctggaaaaac
     3721 cctgccgttc gatgatcgtt gtaccacaaa ccgggcagag agtgttctca tacgggtgat
     3781 tatacacgtt gcccagatag gggaaacgga gcccgagatc ttttgccagc gtatagatct
     3841 tttccagggt ggagagtggc gttgccccgc ggtcggtcat cttgtaatcc gggtggaagc
     3901 cggagaaatg cataggggtt tcagggccga gatgttcgat gacccagcgg ataagggact
     3961 ccatctcttc tttgctgtcg ttgagcccgg gaatgacaag cgtcacggtc tccacgtgca
     4021 tgccgagttc ccgtgcaagg gctgcggaat cgagtaccgg ctgaaggcgt gctccgcaga
     4081 cctttttgta aaaatcatcc gtgaaagcct tgatatcgac ccggaatgca ctaagcatgg
     4141 gttaaagttc acgcagccct tcttctgtga tgtaaccgtt cgtcacatag atagtgccaa
     4201 gtcctttctt gcgggcaagt gttcccatat cgagtgcgta ttcgtgccag atggtgggtt
     4261 cattatatgt ccacgagatg cttgcgcttc cactggcaat tgcccgtttt actccggttt
     4321 ccggcaacag tgtctgcagg cgtgcatcct caaaagaagc ccgcgagatg tgccagttct
     4381 ggcagtgctg gcaatgaaaa ttgcagccaa tgctgcccag tgagtatgaa cgtgtgcccg
     4441 gcaggtagtg aaagagaggt tttttctcaa tcggat
//
LOCUS       DASRLV010000194        13787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_887777,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000194 DASRLV010000000
VERSION     DASRLV010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 13787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13787
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887777"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VFC43_07385"
     CDS             <1..186
                     /locus_tag="VFC43_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HZK31033.1"
                     /translation="PLFGMPILDVEQAKNVVVLKRGQGKGFAGIENDLFYRDNTRMLY
                     GDARETVGRLVQALKKL"
     gene            complement(302..970)
                     /locus_tag="VFC43_07390"
     CDS             complement(302..970)
                     /locus_tag="VFC43_07390"
                     /inference="COORDINATES: protein motif:HMM:NF021414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2115 domain-containing protein"
                     /protein_id="HZK31034.1"
                     /translation="MTRLPDESISSQRINLAQKCRNLQQAKNRGDLGARIADLVLQHS
                     PSDIQRMKMNFSEKIEDLPPKYRDRLAKKINEHLLGTYQRIRLLRQQGVFGTMKEPVT
                     EHQKKYWDMVCMQCPDTEENDASSLRFLKYLISGFCMLVLHEPGHPPGTPFPGGGSVQ
                     VTKGVYYCPVREKANDVDAALCPFCPALQTPEIGYLRPAVDPSEHQKQEFIEHCHKFH
                     NFNG"
     gene            1103..2617
                     /locus_tag="VFC43_07395"
     CDS             1103..2617
                     /locus_tag="VFC43_07395"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HZK31035.1"
                     /translation="MSALFVENLRFLDTTLRDGEQTPGVSLNPDQKFEIANKLADIGI
                     DVIEVGSAAASEGERDAIKLISGAGLRAEICTYVRALHQDIDLAADYGADSVHLVVPV
                     SDLHISKKMRKSREQVAAMAFDAVEYAKSRGLIVELSGEDASRADQVFLQEIFSEGVN
                     RGADRLCFCDTVGLLTPEKVSAWIPPLAQIAPLSIHCHDDLGFALTNTMAALKSGASC
                     AHVTVNGLGERAGNTPLEEVVMALEILYEYRTRIHKEEIYQLSSLVSRLTGVPLPINK
                     AVVGEMAFTHESGIHAHGVMREPSTYESIKPEMIGRKRRIVLGKHSGSASVESALNEM
                     NYKADDLQIKEIVKRIKQLGDSGKRVTDSDLMAIADAVLAIECKPIIKMRQFTAVSGS
                     NMIPTASVTLVVNGQEMTGAATGDGPVDAAMEVLRKCVADVADIRLEEYHVDAIQGGT
                     DALVDVTVRLSKDGKIITSRGARTDIIMASVEAMIAGMNRLLREEHENRGKNPR"
     gene            2589..4265
                     /gene="ilvB"
                     /locus_tag="VFC43_07400"
     CDS             2589..4265
                     /gene="ilvB"
                     /locus_tag="VFC43_07400"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HZK31036.1"
                     /translation="MKTGAKILVESLQREGTEIIFGYPGGVVLPIYDELYDSSLRHIL
                     VRHEQAAAHAADGYARASGRVGVCLATSGPGACNLVTGIATAYMDSVPIVALTGQVPT
                     NLLGNDAFQESDITGITMPITKHNYLVKSAGDLSRVVQEAFYIAGTGRQGPVLIDIPK
                     DVSTGMAADVKLPEKVTLRGYNPTYKGHKRQIEKALDLLDHSERPLIYAGGGIISSNA
                     SSELVEFATLASIPVTTTLMGIGCIPCHHPLNLGMLGMHGTEYANFAVTECDLMIAIG
                     ARFDDRVTSKIDTFAPHAKIIHIDIDPAEIGKNKRIDIPIVGDVKVVLLDVLALMKKR
                     DAHEVWLKKIKHWKQHHPLKCPKNGSLHPQYIIRTLSDLVKDKAVIVSEVGQNQMWTA
                     QHFCFHNPRTWITSGGLGTMGYGLPAAMGAHFARPDVPVFDIAGDGSIQMNIQEFGTI
                     ASNKIPVKVAILNNMFLGMVRQWQELFYDHRYSFTELPPVDFVKIANAYGIEGMRVES
                     PDEVLPALQASLDCDGPFVMDFRIEREENVFPMVPGGAAINEMIVGHPRS"
     gene            4262..4765
                     /gene="ilvN"
                     /locus_tag="VFC43_07405"
     CDS             4262..4765
                     /gene="ilvN"
                     /locus_tag="VFC43_07405"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HZK31037.1"
                     /translation="MKPHTLSILVENKAGVLSRVTGLFSRRGFNIESLAVGPCEEPDM
                     SRITIVVIGDDEKIEQVMKQLNKLIDIIKVSDLTDSERVERELALIKVTAELGTSRAE
                     IMQIASIFRAQIVDVGAKTLILQVIGDTDKIDALEKLLRQYGIKEFVRTGTIGILRGS
                     KTVTSGK"
     gene            complement(4964..5347)
                     /locus_tag="VFC43_07410"
     CDS             complement(4964..5347)
                     /locus_tag="VFC43_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZK31038.1"
                     /translation="MNRAKILVVEDEPEVTETLKLTLKKLGYTVVGCENTAENAIHSA
                     GELHPDIVLMDIELAGKMDGVQAADTIRKKYHIPVIYITAICDTKTLERVGASIPYGF
                     ILKPFKEDELHTVIEIALETIAKKV"
     gene            5714..7219
                     /locus_tag="VFC43_07415"
     CDS             5714..7219
                     /locus_tag="VFC43_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315584.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HZK31039.1"
                     /translation="MIDPVAAALVVIYFIAMVGIGIWASKKIHNMSDYVVAGRSLDFW
                     VFTMLMIGSVCSGMSLLGVSGLGYKFAWPTIWEQLAVPLSIAFCIIFFGVKMHTVAKK
                     SGYMTVQDYLAHRFESQTSLRSMSATAGIVVSMIYLAGQYTAISIVLMWLFGLPHWLA
                     LLIGAAIVTIYTVLGGLYAVAWTTLIQGLILIVGVLIMAPIVIMAAGGFTHINEVMAA
                     VNPTNVDPWVTGAGAVFTPEYIFSFVLLLTIGLACAPHVINNILCVKDVKYFKWAPLV
                     AFGIYAVVMFLLKFAGFAGIVLVNDGIVTLPKVPNASDFIFVYGVQAAMPSMFLWGIF
                     AVIVLAAVMSTTDRLMLTIGTYFSWDIYKKILRPDAPDKQVLLVSQISVFIAAAVTLL
                     LALNPPDMLVWLIWAGIGIMFATFSVPLLAGLYWRRATREGALVSMALGLIVSLAFGG
                     ISYFKLAKLPVHFSLYAFVIAVIAMIVVSLLTKPTSEKVLDETMTGWYISK"
     gene            7271..8584
                     /locus_tag="VFC43_07420"
     CDS             7271..8584
                     /locus_tag="VFC43_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK31040.1"
                     /translation="MKTAILGGGLSGLTLARLMHEEGQEVVVLEAEAEYGGLCRSKTK
                     QGFTFDTGGSHIIFSRDAEVLSFMRRMIAGNEQLNNRNTKIFYKQQFVKYPFENGLSD
                     LPPEDRFLCIHEFIRTLIAVEKDELAPPSNFREWIYYTFGKGIAECYMLPYNEKIWKY
                     PTDRMSLHWVDGRIPRPPVEDIIKSAVGINTEGYTHQSVFSYPLDSGIEALVRAIAKP
                     IAPFIRTGFRVTSLAKSGEEWHISNGTEIIRADQCICTMPVQHLLPCLKGVPGNVQNA
                     INALKYNSLVCVNIGIKGSIPPVSWLYVPDHAVGRTNRISFPSGYSRNVAPDGCGAIL
                     AEITHQPVDEISRMSDEDLIDEVTDMLDKMHILKKDQIVYSSVERQPFAYVVYDLDYQ
                     KNIALVREYFTSISLPLVGRFAQFEYLNMDGCIRSVMDFLSARQP"
     gene            8650..9348
                     /locus_tag="VFC43_07425"
     CDS             8650..9348
                     /locus_tag="VFC43_07425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZK31041.1"
                     /translation="MISVIIPTFNEEENIAQCLVSLTHQTTPRNEYEIIVVDGGSKDE
                     TCSIAGKYADKVFVQTSKKVGGARNDGIMAAQGDIVATTDADCILPPNWIELIKKDFL
                     DERVAQLYGPVYPIEVGIGNKLSLLLANTFSRIGYYSQTFYYTLGCNTAFRKSSFIKA
                     GMYRCIDAGDDLEIALRMKDQGRVVFDGRLKVGFSMRRYEQYGTLKSLYEWVYIVSHG
                     GETQKYSYTQKKYK"
     gene            9393..9821
                     /locus_tag="VFC43_07430"
     CDS             9393..9821
                     /locus_tag="VFC43_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HZK31042.1"
                     /translation="MVQNHREAYDEQTVSDTISAFNQSEFALLLGMQIVEAYDGYARV
                     VMDCTGKNNKKNIAHGGAIFALADQAFGIAANCGGTDRVAVSIHIQYIAPATGSLVAI
                     AHRIADNGIHSLFRVVVFQEERIIAEFDGVAIQVPKLFRQ"
     gene            9853..10140
                     /locus_tag="VFC43_07435"
     CDS             9853..10140
                     /locus_tag="VFC43_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31043.1"
                     /translation="MPDGNVKKKPHFRDHKYNPQVISCTFNDLVNIKGRNGKRAPMAG
                     NPCDTCMKDCVGGIIYGNCQQFLHWRREINKIVVCVDSLINPKRTGHFHEI"
     gene            10338..11408
                     /locus_tag="VFC43_07440"
     CDS             10338..11408
                     /locus_tag="VFC43_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HZK31044.1"
                     /translation="MPTMKIRGGDLDLVEYEFNSFFPGMNLKTLGYEKKTYALKPKSG
                     KITVTAPARIHLTVLDMNRFAPNHPGGGGIGFAIQVYCTAEVECIKEGLEIDYSRSAI
                     LENFVAVFKKTVGYSGGFKIKATDHQHKHVGLGSTSTIMIAVATGMNEALGRPLTNDQ
                     LRYLIGHNYVEETVDGNVAFGFETGVGPAVSTYGGMAILGDELTLVYHHPFAPGKNVF
                     IIIPPTNISSAGTQEFDLLMNKARNLDYRDRELKAYLFLMDLVPALEHDDLKKAGDVI
                     WEIEFRGSKRAEVEHHSYEIYHYMSQLRAASLEFVGMSSVGPSIAVVTGLDKKSLEKI
                     LKPLGLEIAISTKVDNEGLQIS"
     gene            complement(11613..12284)
                     /locus_tag="VFC43_07445"
     CDS             complement(11613..12284)
                     /locus_tag="VFC43_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HZK31045.1"
                     /translation="MIDSLITIFLNLDQNLSAVVNDYGVWTYFILFFIIFFETGFVIL
                     PFLPGDSLLFMCGAAAASGIMDLQWVLTAIIIGAVLGDTANYWIGNYLGIHVFMERFP
                     TLVKKEYIDRTYGFFEKYGGATIFVARFVPVVRTFAPFLAGIGSMNYSRFLIYNILGG
                     VTWAFVIVLGGYFFGTIPVVKENMSLLIVLVILVTAVTVFAVLAGIVSAYLNKRKTMH
                     KHGRK"
     gene            complement(12414..13034)
                     /locus_tag="VFC43_07450"
     CDS             complement(12414..13034)
                     /locus_tag="VFC43_07450"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZK31046.1"
                     /translation="MEHFTGGWEDDYSRRGFLWGGATQDLPPVPAGSHVLEIGCGNGK
                     TLSTMIQRGWDVTGIDFSARAVTMSRERCAGTSSFEFMVADTIHLPFKNSSFDAVFAI
                     HIFGHLHEPERKHLPNDLENILKPGGILFFSDFSTEDFRYGSGSETETATFMRGTGII
                     THYFSRQEVLDLFSNLTLVWIRIHEWPLRILGKNLVHSEIKAVFTK"
     gene            complement(13130..13504)
                     /locus_tag="VFC43_07455"
     CDS             complement(13130..13504)
                     /locus_tag="VFC43_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31047.1"
                     /translation="MQSGMVLLFVYNIERGNLSDMPDYHQTVSSVKTPRCNLFALIYS
                     PIGMKKAWKRFISDLGFPCRYLYHDEFQQISGEQYPPLPAVYIHSGKSFHELISSEEI
                     NRTGSVDTLIGLVTQRLKQYLT"
BASE COUNT         3606 a         3169 c         3346 g         3666 t
ORIGIN      
        1 ccactcttcg gtatgccaat cctcgatgtc gaacaggcaa agaacgttgt tgtcctcaaa
       61 cgcggccagg gaaagggttt cgccggtatt gagaatgacc tgttctaccg ggataacacc
      121 cggatgctct acggtgacgc ccgggagacc gtaggccggc tcgtgcaagc cctcaaaaaa
      181 ctataaacct tacgggcata cggcaggccg gaattacgac ctgccgcctg tgtgatctat
      241 gcagtaccgg gtgaagaata aggcagaata tactcgttga atactcctca attccacctt
      301 ttcagccgtt gaagttatgg aatttatggc agtgttctat gaactcctgc ttctgatgtt
      361 cgctcggatc gactgctggc ctgagatacc ctatctcagg agtctggagc gccggacaaa
      421 acggacagag tgctgcatca acatcattgg ctttttcccg gacagggcaa tagtaaacac
      481 ccttggtcac ctggactgac cctcccccgg ggaagggtgt gccgggcggg tgaccgggct
      541 catgaagcac cagcatgcag aacccggaaa tgaggtattt caaaaacctg agggaagaag
      601 catcgttttc ttcagtgtca gggcattgca tgcataccat gtcccagtat tttttctggt
      661 gttcagttac cggttctttc atagtcccaa agacgccttg ctgccgcaac aggcggatcc
      721 tctggtaggt tcccagaaga tgttcattga tcttttttgc aagcctgtca cgatatttag
      781 gtggcagatc ctctattttc tcactaaaat tcattttcat tctctgtata tcagaaggtg
      841 aatgctgtaa gacgagatct gcgatacgag cccccagatc gccacgattc tttgcctgct
      901 gcagattccg gcacttctgt gcaaggttta ttcgttgact actgatagac tcatctggca
      961 agcgggtcat aaggctggtg attctttcac tgtcatacta tgcgaggatg aatcatataa
     1021 tttagatgaa tcaggtattt tctctatacg aacgtttttt atatttctct tcccaagttc
     1081 gtatcatggt gatttgccat gagtgagtgc tttatttgtt gaaaacctgc gttttttaga
     1141 tactacgtta cgggacgggg aacagacacc gggcgtttcg ttaaaccctg accagaaatt
     1201 tgagatcgcg aacaaacttg ccgatattgg cattgatgtg attgaagttg gttctgctgc
     1261 cgcatccgaa ggcgagcggg acgctatcaa gctcatatca ggagccgggc ttcgcgcaga
     1321 gatttgcaca tatgtccgtg cacttcatca ggatatcgac cttgccgccg attatggggc
     1381 tgattcggtc cacctggtag tgccggtgag tgacctgcat atctcaaaaa agatgcgtaa
     1441 atcccgtgag caggtcgcgg cgatggcatt tgatgccgtg gaatatgcga agagccgcgg
     1501 gcttatcgtc gaactttccg gggaggatgc gtcccgggca gaccaggtgt tcctgcagga
     1561 aatcttttct gagggagtta accgtggagc tgaccgcctc tgtttttgcg atacggtagg
     1621 acttctgaca ccggagaagg tatcagcctg gatacccccg ctcgcacaga ttgctcctct
     1681 ctccatccac tgccatgatg atctcggttt tgctcttacc aatacgatgg ctgcccttaa
     1741 atccggggct tcctgtgccc atgttaccgt aaatggtctg ggagagcggg cgggaaatac
     1801 tccgcttgag gaagtggtga tggcgctgga aatcctctat gaataccgca cgcggatcca
     1861 taaagaggag atttaccagc tttcctccct tgtttcgcgc ttgaccggtg tcccgctgcc
     1921 tatcaataaa gcagttgttg gtgagatggc gttcacccat gaaagtggca ttcatgccca
     1981 tggtgtcatg cgggaaccat cgacctacga atcgatcaaa cccgagatga ttgggcgcaa
     2041 acgccgtatt gttctgggaa aacattccgg ttctgcttct gtcgaatctg ccctcaatga
     2101 gatgaattac aaagcagacg atctccagat aaaagagatc gtgaaacgga tcaagcagct
     2161 gggcgattca ggcaagcgcg tgactgactc ggaccttatg gcaattgctg atgcggtgct
     2221 tgcaattgag tgtaaaccga taattaaaat gcgccagttt actgccgtat caggcagcaa
     2281 tatgatcccg actgcttcgg tcacgcttgt cgtaaacggt caggaaatga ccggtgctgc
     2341 aaccggggac ggtcctgttg atgcggcgat ggaagtactc cggaaatgcg ttgccgatgt
     2401 ggccgatatc aggttagaag agtaccatgt ggatgcgatc cagggtggta ctgatgcact
     2461 ggtcgacgta acagtaagat taagtaaaga cgggaagata attacttcac gcggcgctcg
     2521 taccgacatc attatggcca gtgtcgaagc gatgatcgcc ggcatgaaca gactactgag
     2581 ggaagaacat gaaaaccggg gcaaaaatcc tcgttgaatc actccagcgc gaagggaccg
     2641 agatcatctt cgggtatccc ggcggagtgg tgctgccgat ctatgatgaa ctctacgact
     2701 cgtcattgcg gcacatcctt gtacggcacg aacaggctgc agcgcatgcc gcggacgggt
     2761 atgcgagggc aagcggtcgt gtaggcgtat gcctcgccac gtcagggccc ggtgcatgta
     2821 acctcgttac cggcatcgcc acggcttaca tggactcggt ccccattgtg gccctgaccg
     2881 ggcaggtacc gacaaacctg ctcggaaacg atgcgtttca ggaatcggac atcaccggta
     2941 ttaccatgcc gatcaccaag cacaactacc tcgtgaaaag tgcaggtgat ctcagccggg
     3001 ttgtccagga agcgttctat atcgccggta ccggcaggca gggaccggtt ctgatcgaca
     3061 tccccaagga cgtcagtact ggtatggccg cggatgtgaa gttgccggag aaggtcacgc
     3121 tccgtggcta taatccaacc tacaagggcc acaaacgcca gattgaaaaa gcgctcgatc
     3181 tgctcgatca ttccgagcgc ccgctcattt atgccggagg gggcattatc tcatccaatg
     3241 cctcgtccga actggtggaa tttgcgaccc ttgcatctat tccggtcacg accaccctga
     3301 tggggatcgg atgcatcccc tgccatcacc cgctcaacct cggcatgctg gggatgcacg
     3361 gcacggaata cgcgaatttc gcggttacgg agtgcgatct catgattgcg ataggagctc
     3421 ggttcgatga ccgggtaacc agcaagatcg atacctttgc accccatgct aagatcatcc
     3481 atatcgatat cgacccagcc gagatcggca agaacaagcg gatcgatatc ccgattgtcg
     3541 gggatgtcaa agtggtgtta ctggacgtgc tcgcgctcat gaagaaacgg gacgcccatg
     3601 aggtctggtt aaaaaagatc aagcactgga aacagcacca cccgctcaaa tgcccgaaga
     3661 acggctcgct gcacccgcaa tacatcatca ggaccttaag cgaccttgtc aaagacaagg
     3721 ctgttatcgt gtccgaagtc gggcagaacc agatgtggac agcgcagcac ttctgcttcc
     3781 acaatccccg aacctggatc acctcagggg gccttggcac gatgggatat ggtctcccgg
     3841 ctgcgatggg ggcacatttt gcccgccccg atgtcccggt cttcgatatt gccggcgatg
     3901 gcagtatcca gatgaacatc caggaattcg gtaccattgc ctcgaataag ataccggtca
     3961 aggtcgccat cctgaacaac atgttccttg gcatggtccg gcagtggcag gagctcttct
     4021 acgaccaccg gtattcattc accgaactcc cgcccgttga cttcgtcaag attgccaacg
     4081 cttacgggat cgaagggatg cgggttgaat cacctgatga ggtgctcccg gcattgcagg
     4141 cctctcttga ctgtgacgga ccgtttgtga tggatttccg gatcgaacgc gaagagaacg
     4201 tatttcctat ggtcccgggc ggggctgcga tcaacgagat gattgtaggg catccccgat
     4261 catgaagccg cacacgttat ccatccttgt cgagaacaag gccggtgtac tctcgcgggt
     4321 caccggtctc ttctctcgcc ggggattcaa catcgagagc cttgcagttg gcccctgcga
     4381 ggagccggat atgagcagga tcactatcgt agtcatcggt gacgatgaaa agatcgagca
     4441 ggtgatgaaa cagctcaaca agctcatcga tataatcaaa gtctcggatc tgacggatag
     4501 cgagcgggtg gagcgggagc tcgccctgat taaggttacc gctgaactag ggacttcccg
     4561 tgcagagatc atgcagatcg cctccatatt ccgggcccag attgtcgatg tcggcgcaaa
     4621 aaccctgatc cttcaggtca taggggatac agataagatc gatgcacttg aaaaactgct
     4681 gcgccagtac gggatcaagg agtttgtccg gacgggaaca attgggattc tccgtggatc
     4741 aaagacggtt acgagtggga aatagttcat cattcacaaa aaaatagttc tttttttaca
     4801 gcacttgtga taaataaatc aggtagactg atctggtgtt tcatatccag tttgcttatc
     4861 gcactttctt aaagcgatat tggcaaaaag tgtgtaagag taaaaacaat ttcatccggc
     4921 cagcatcctt gtggcgcaga taaaaattgg agcgggttga gaattacact tttttagcga
     4981 ttgtctcaag tgcaatctcg attactgtgt gaagctcatc ctctttgaag ggtttcagga
     5041 tgaaaccgta ggggatactt gcaccgaccc gttccagtgt tttggtatca cagattgcag
     5101 taatataaat tactggtatg tgatattttt tcctgatagt atcggctgcc tgtacgccat
     5161 ccatctttcc ggcaagttct atgtccatca ggacaatatc cggatggagc tcacccgcgg
     5221 aatgaatggc attctcagcc gtgttctcac acccgacaac agtgtagcct aattttttta
     5281 gtgttaattt cagagtttcc gttacttcgg gttcatcttc aacaaccaat atttttgccc
     5341 ggttcataac tcaacgttac tgattttgtc aagggattat tttaattgat tgattcattc
     5401 acagagtttt tagtgtcact tcatttcagg gtcattccag aatatgcaag gatccaaacc
     5461 gattcatcct ttcttcaata tacataatct tatttggcag tactgttttc ccgtcgtaaa
     5521 aaaaatatta ttagttttat attcaccaat tatttcggtg atttttcgat tatgttatat
     5581 gttagcatac cacaaagtat tttgatataa tgtttggatt gccgattttg actatcgctt
     5641 tggtcggggg tgcttttctc ctgattcttc tcgcattgct gtactgggga ctaaccttta
     5701 aggggacctc ctcatgattg atcctgtcgc agccgctctt gtagtgattt attttatcgc
     5761 tatggttgga atcgggatct gggcttcaaa aaagatccac aatatgtcgg attatgttgt
     5821 agccggtcgt tctctggatt tctgggtatt caccatgctc atgatcggtt cggtctgttc
     5881 aggcatgtcc ctgctgggtg tttcggggct gggttacaaa tttgcctggc cgactatctg
     5941 ggagcaactt gctgtaccgc tttcaattgc attctgtatc atcttctttg gtgtcaagat
     6001 gcacacggta gcaaaaaagt ccggatacat gactgtgcag gattatctgg ctcaccggtt
     6061 tgaaagccag acctctctca ggagtatgag tgcgaccgca gggattgtcg tatcgatgat
     6121 ctatcttgct ggacagtata ccgcgataag tatcgttctg atgtggctgt tcgggctccc
     6181 gcactggctg gcactcctta taggggcggc aatcgtgacg atttatacag ttctcggagg
     6241 tctctatgcc gtcgcctgga caacccttat tcagggacta atcctgatcg ttggtgttct
     6301 catcatggcc ccgattgtta tcatggctgc tggagggttc acccatatca atgaagtaat
     6361 ggctgccgta aacccgacaa atgtagatcc ctgggtgacg ggagccgggg cggttttcac
     6421 tcctgaatat atcttctcgt ttgtcctgct gctcacgatt ggtcttgcct gtgcccccca
     6481 tgtcatcaac aacatcctgt gtgttaagga tgtgaagtat ttcaaatggg cacccctcgt
     6541 cgcgtttggg atttatgcag tggtgatgtt cctgttaaag tttgccgggt ttgccggtat
     6601 agttcttgtc aatgacggga ttgttacatt gccaaaagta cccaatgcat ccgatttcat
     6661 ctttgtttat ggcgtacagg ctgcgatgcc cagtatgttc ctgtggggta tatttgcagt
     6721 gattgtgctt gctgcagtca tgtcaacaac cgaccgtctc atgctgacca tcggaacgta
     6781 cttctcctgg gatatctaca aaaagatcct cagacccgat gcaccggata aacaggttct
     6841 tctggtcagt cagatatctg tgtttattgc agctgcagtt acactccttc tcgctcttaa
     6901 ccctccggat atgcttgtct ggcttatctg ggcaggaata ggaataatgt ttgccacatt
     6961 ttcagtcccg ttgcttgcag gactctactg gcggcgggca acccgggaag gggcactggt
     7021 ttctatggcg ctgggattaa ttgtgtcgct tgcatttgga ggaatttcct acttcaagct
     7081 ggcaaaatta ccggtgcatt tttcccttta tgcgtttgtc attgcagtca ttgccatgat
     7141 tgttgtcagc ctattgacaa aaccgaccag cgagaaggtt cttgatgaaa cgatgaccgg
     7201 ctggtatatt tcaaaataaa cttttttgca caatcattca tataaattct gcacgcctac
     7261 gtaatgagca gtgaagactg caattcttgg cgggggactt tcggggctca ctctggctcg
     7321 gctgatgcat gaagaaggtc aggaagtcgt tgttctggaa gcggaagcgg agtacggggg
     7381 gctctgtcgt tcaaaaacaa aacagggatt tacatttgat actggtggtt cacatatcat
     7441 cttttcgcgg gatgctgaag tcctctcttt tatgcgtagg atgattgccg gcaatgaaca
     7501 gctcaataat cgcaatacca aaattttcta taaacaacag ttcgtcaaat acccctttga
     7561 gaacgggctt tctgatctgc ccccggagga caggtttctt tgtatccatg aattcatcag
     7621 gaccctgatt gctgttgaga aggatgaact ggctccgcca tcaaatttcc gtgaatggat
     7681 ctactacacg ttcgggaagg gtattgccga atgctacatg ctcccctata atgagaaaat
     7741 ctggaaatac ccgacagacc ggatgtccct gcactgggtt gatggccgta taccccgtcc
     7801 tccagtggaa gacataataa agtccgcagt cgggataaat accgaaggct acacccacca
     7861 gtcagttttt tcctatcctc tcgatagcgg gattgaagcc ctggtccggg caattgcaaa
     7921 gcccatcgca ccattcatcc ggaccggttt cagggttacc tcgctggcaa aatcaggtga
     7981 agaatggcat ataagcaatg gcacggaaat tatcagggca gatcagtgca tctgtactat
     8041 gcctgtacag cacctgttac cgtgccttaa aggcgtccct ggaaatgtac aaaatgcgat
     8101 aaacgcattg aagtacaatt cattggtttg tgtgaatatt ggtatcaaag gatccatacc
     8161 tcctgtttca tggctctacg taccggatca cgcggtcggg agaacaaacc ggatatcttt
     8221 tccttcgggg tacagccgca atgttgcccc tgatggctgc ggtgcgatcc ttgctgaaat
     8281 cactcaccag ccagttgacg agatttcccg catgtctgat gaggacctca ttgacgaggt
     8341 aactgatatg ctcgacaaaa tgcatattct gaaaaaagac cagattgtgt actcatctgt
     8401 tgaaaggcaa ccgtttgcgt atgtagtata tgaccttgac taccagaaaa atattgcact
     8461 ggtaagggaa tattttacca gcataagtct gccgctcgta gggaggttcg cacaatttga
     8521 atatctgaac atggatggct gtatcagaag cgtcatggat tttctgtcag cccggcaacc
     8581 gtagtgttcg ttagctccat cacgcttata atacgaagcc atcaacaatg tacaggagat
     8641 tttatcccca tgatatcggt cattattccg acctttaatg aagaagagaa catagctcaa
     8701 tgtcttgtct ctcttaccca ccagactact ccgaggaatg aatacgagat cattgtagtg
     8761 gatggaggat ccaaagatga aacctgcagc atcgcgggga aatatgctga taaggtattt
     8821 gtccagacaa gcaaaaaagt cggcggagcc agaaatgatg ggattatggc agcacaagga
     8881 gatattgttg ctaccactga tgccgactgt atacttcccc cgaactggat tgaactgata
     8941 aaaaaagact ttctggatga acgcgttgcc cagctgtatg gtccggtata tccgattgag
     9001 gtggggatag gcaataaatt atccctgctt cttgccaata cattctcccg tattggctat
     9061 tacagccaga cgttttatta cacgctcggg tgtaataccg ctttccggaa atcttctttt
     9121 ataaaagccg ggatgtaccg ttgtatagat gccggagacg acctggaaat agccttacgc
     9181 atgaaagatc agggcagggt cgtatttgac gggcgactta aggtaggatt ttcaatgcgc
     9241 cgctatgagc agtacgggac cttaaaatca ctctatgaat gggtgtatat tgtatcccac
     9301 ggtggcgaaa cccagaaata ctcctatacc cagaaaaaat acaaataata tgctgttctc
     9361 cggtccgtta ttttttgata ccaggtgagt atatggtaca aaatcatcgg gaagcatatg
     9421 atgaacaaac ggtttccgat acgatttctg catttaatca aagtgaattc gccctgcttc
     9481 tgggaatgca gattgttgag gcttatgacg ggtatgcccg agttgtcatg gactgtaccg
     9541 ggaaaaataa caaaaaaaac attgcccacg ggggggcaat atttgccctt gcagaccagg
     9601 cattcgggat tgcggcaaac tgcgggggaa cagaccgtgt cgcggtttca atccatatcc
     9661 agtatattgc tccggcaacg ggttctcttg tcgcaattgc ccatcgtatt gccgacaatg
     9721 gaatacactc cttatttcga gtcgttgtat ttcaggaaga acgtataatt gcagaatttg
     9781 atggggtggc gatccaggtc ccaaagctgt ttcggcaatg aataaaaaac ccgtttttct
     9841 aacagaaaac atatgccgga tggcaatgtt aaaaaaaaac cccatttccg ggatcataag
     9901 tataaccccc aggttatcag ctgtaccttt aatgatctcg tgaatataaa aggccggaat
     9961 ggtaaacgtg cacctatggc aggaaatccg tgcgatacct gtatgaaaga ctgtgttggc
    10021 ggtatcatct atggcaactg tcagcaattc ctccactggc gcagggaaat caataagata
    10081 gttgtttgtg tagactctct gatcaatccg aagcggactg gtcatttcca cgaaatctaa
    10141 gattttgtaa aaacgtttta ccgtcgcttt tcttacctta cggtatactt tggcttatgt
    10201 ccaaaagagt cagatcatga tactttttat ttgaggaaat aattccagtc atggtgtttt
    10261 tcatgaagcg tttattttct gattgaaaac tggggaaaaa catattcctt ggtatgtact
    10321 aacaggaatt gttcattatg ccaaccatga aaataagggg cggagacctc gatctggtcg
    10381 aatacgaatt caattctttt ttcccgggaa tgaatcttaa aactctcgga tatgagaaga
    10441 agacctatgc actgaaacca aaatcaggaa aaattacggt tacagctcct gcacgaatac
    10501 atctgaccgt gctcgacatg aaccggtttg cgccaaatca tcccgggggt gggggaattg
    10561 gttttgcaat ccaggtatat tgtacggcag aagtagagtg cataaaagaa ggtctggaga
    10621 tagattacag ccgttctgcc atccttgaaa attttgttgc ggtcttcaaa aagaccgtgg
    10681 gatattcggg aggctttaaa atcaaggcaa ccgatcatca gcataaacat gtcggactcg
    10741 gatccacaag taccatcatg attgcagtag caaccggaat gaacgaagca cttggccggc
    10801 cattgacaaa cgaccagttg aggtacctga ttgggcataa ttatgttgag gaaacggttg
    10861 atggaaatgt tgcctttggt tttgaaaccg gtgtcgggcc agctgtgagt acctacggag
    10921 gaatggcaat actgggagat gaactgactc ttgtttatca ccatcctttt gctccgggaa
    10981 aaaatgtatt tattattatc cctcccacaa atatctcttc agccggaact caggaattcg
    11041 atctcctgat gaacaaggcc cgtaatctcg attaccggga ccgggaactc aaagcgtatc
    11101 ttttcctcat ggaccttgtt ccagctcttg aacacgatga tttaaaaaaa gcaggagatg
    11161 tgatctggga aatcgaattc agaggttcaa aaagggcaga agtagaacac catagttatg
    11221 agatctatca ttatatgagt caactcaggg ccgccagcct tgaatttgta ggtatgagct
    11281 cagtaggtcc atcgattgca gttgtaacgg gactggataa aaaatcactt gaaaagattc
    11341 tcaaacccct tggtctggaa attgctattt cgacaaaagt tgataacgaa ggtttgcaaa
    11401 tctcttaacc ccccattttt gccattcatt tcatcaggcg ggatgaatga tctgaatatg
    11461 gttaccatat gaattacttc tgctccccgc gatatccctt gtggtttttt cctcatgagt
    11521 tacctgaata acccggacat gtaatcccct cgagacaaaa aaattcccga tactgtgttt
    11581 atgaaacagc gtcactcaga aattttaagt tcttatttcc tgccatgttt atgcattgtt
    11641 ttccttttat tcaggtatgc ggaaacaata cctgcaagta cggcaaaaac ggtcacggcc
    11701 gtaacaagaa tgacaagaac tatcaacagg ctcatatttt ccttaacaac aggaatagtc
    11761 ccgaaaaaat atccgccgag cacgataacg aatgcccagg tcactccccc cagaatatta
    11821 tagataagga accgtgagta attcatagac ccgatcccgg caaggaaagg tgcaaaagta
    11881 cgaacaacgg ggacgaaccg tgccacaaag atcgttgctc ctccatattt ttcaaaaaat
    11941 ccgtaggtcc ggtcaatata ttcttttttg acaagagtcg gaaaccgttc cataaaaaca
    12001 tgaatgccga gataattgcc aatccagtaa ttcgcggtgt ccccaaggac ggcaccgatg
    12061 ataattgcag taagaaccca ttgaaggtcc atgatgccgc ttgctgctgc ggctccacac
    12121 atgaaaagga gcgaatctcc cggaagaaat ggcaggatga caaaaccggt ctcaaagaaa
    12181 atgatgaaaa aaaggataaa atacgtccag accccgtaat cgttgacaac agcggataag
    12241 ttctggtcaa ggttaaggaa tatagtgatt aaggaatcta tcataattca caaaaaatca
    12301 ttcgttcagt cgctatatta accagcgcag ttcctgaaac attttttatc tgaaatgatt
    12361 tgtggctgga atattttttt aagaaataac tgatggggga aattatgcct tctttatttg
    12421 gtaaaaactg ctttaatttc agagtgaacc aggttctttc ctaaaatccg cagcggccac
    12481 tcatgaatcc ggatccagac cagtgtcagg ttggaaaaaa gatccagtac ttcctgacgc
    12541 gaaaaataat gagtaataat tcctgtccct ctcataaatg tcgcagtttc tgtttcagat
    12601 cccgagccat accggaaatc ttcagttgaa aagtcagaaa agaacaggat acctccgggt
    12661 ttgaggatat tttccagatc gttagggaga tgttttcttt caggttcgtg caggtgtcca
    12721 aaaatatgaa tggcaaagac ggcgtcaaat gaactgtttt taaacggtaa atgaatggta
    12781 tctgctacca tgaattcaaa agacgatgta cccgcacagc gttccctgct cattgttacg
    12841 gctcgcgcag agaaatcgat acctgtcaca tcccaccccc gctgaatcat tgttgagagt
    12901 gttttgccgt tcccacaccc gatttcaagc acatgcgatc ctgctggtac aggtgggaga
    12961 tcctgtgttg ccccacccca tagaaatcct cggcgggaat aatcgtcttc ccagccacca
    13021 gtgaaatgtt ccataataca tgtctttact aaaatatcag ggtacggtct cgattaattc
    13081 gggcaaggaa tttgttggcc tccttttgtt taagcaccat gaaaacgagt taggtcaggt
    13141 attgtttaag cctttgagtc acaagaccga taagagtatc tactgaacca gtccgattga
    13201 tttcctccga actgataagt tcatgaaaag actttcccga atgtatgtag actgcaggca
    13261 aaggtggata ttgttctccg gatatctgct ggaattcatc atggtagaga taacggcagg
    13321 gaaaacccag atcgctgatg aaacgtttcc atgctttctt cataccaata ggactgtaaa
    13381 tcagcgcaaa cagattacaa cgcggggttt ttacggatga caccgtctgg tggtaatcag
    13441 gcatatctga caggttgcct ctctcaatat tatacacaaa cagcaggacc attccggact
    13501 gcatcgtaca ttcccatttc tttatgatga cttaaggatt ggggtttttt aataaccggg
    13561 tcagtcggta caaagaacga ttaggtcgct tttcggcatc agggttcctg ataatatttc
    13621 tggcattggg aaatggcaaa ggcgataaat tatgatatac atccgtgcta taatacatag
    13681 aaacgcgtac acgagaaggt atactcaatg cttgacatca ggttcgtacg atcaagcccg
    13741 gatatcgtca gggctgatct tgaaaaacgc aatgatacag agaagct
//
LOCUS       DASRLV010000195         5948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1203551,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000195 DASRLV010000000
VERSION     DASRLV010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203551"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VFC43_07460"
     CDS             complement(<1..282)
                     /locus_tag="VFC43_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31048.1"
                     /translation="MDLGSTFFYKYRRFVKTLKIYLLLAGPGLIVMLADNDAGGITTY
                     TVTGAKFGYGLIWFLLLLYVTQQEEERSPWISDQPFFTNIAGLSRRSRYT"
     gene            complement(604..996)
                     /locus_tag="VFC43_07465"
     CDS             complement(604..996)
                     /locus_tag="VFC43_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HZK31049.1"
                     /translation="MISHSRVIFSSACAILLVLACSFSGCTSNQSPAPSTPTTPTPVA
                     GLNSIAIKNFAFSPAILTIKTGTTVTWMNQDGAPHKIASDPGSVVAFTSESLANGASY
                     QFTFNQTGTYNYYCTIHPTMKGTIIVQN"
     gene            complement(1101..3677)
                     /locus_tag="VFC43_07470"
     CDS             complement(1101..3677)
                     /locus_tag="VFC43_07470"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HZK31050.1"
                     /translation="MPFLDLDSVKGLSCEEAAEKIRTEGYNELPESKKRGIFWIIFEV
                     IHEPMFILLVASGLIYFILGDLTEGIMLMSFVVVIIGITVYQEQKTERALEALRNLSS
                     PRALVIRDGEQKRIAGREVVTGDMLVLVEGDRVPADGVLLSSNNVTVDESLLTGESVP
                     VRKVPWTDGLHAERPGGDDQPFVYSGTMVVQGQGLAEVKSTGTRTEMGKIGTVLQTVE
                     RGETRLKSEISRIVRTIAIVGIFLCAVIVVVYGLSRGNWTEGFLAGITLAMAILPEEF
                     PVVLTIFLALGAWRISKKNVLTRQVPAIETLGSATVLCVDKTGTLTQNKMTVHSFFAA
                     GQLCVHDAAGKNSVPEICHEVAEFAILACKKDPFDPMEKALFHLRDGEFGTTEHIHTG
                     WELITEYPLSPELLAMSNVWQSPAGNEFVIAAKGAPEAIADLCHFDEAKRQALATDID
                     SMAVQGLRILGVAKALFTKPELPDGQHDFTFTFLGLVGFADPVRPLVADAVRECYTAG
                     MRVIMITGDYPLTAQNIGRQIGLAHTDQCITGSELEQMSEAELKERIRTATLFARAVP
                     EQKLRIVNALKANGEVVAMTGDGVNDAPALKSADIGIAMGGRGTDVARESSSLVLLDD
                     NFTSIVSAVRLGRRIFDNLKKAMAYIFSVHVPIAGMSLMPVLFNMPLVLLPVHIVFLE
                     LIIDPACSVVFESEKEEPDVMNRPPRHRDEGLFTKKTLALSLLQGFVVLAVVLTIYAD
                     AILRGFGEAEVRTLTFTTIVIANLCLILTNRSWSETMLTTLRTPNKAMIWVFAGTISC
                     LILVLFIPFLQDLFRFGSVPFADLILCVIAGGLSVVWFECYKYWNKRVRKRDAVPSSP
                     GT"
     gene            3957..4361
                     /locus_tag="VFC43_07475"
     CDS             3957..4361
                     /locus_tag="VFC43_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZK31051.1"
                     /translation="MKKGFAANLEEETRKNTDFRRVMYTGKHSQLVLMCLKPNEEIGE
                     ETHDDVDQFFRFEEGEGAVIIDGVKHVVKDGSGVVVPSGAKHNVVNTSKVKNLKLYTI
                     YSPPEHQDKVVRKTKQEAMAKEEHYDGKPTEK"
     gene            4582..4914
                     /locus_tag="VFC43_07480"
     CDS             4582..4914
                     /locus_tag="VFC43_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31052.1"
                     /translation="MASIVFTLLSFVIALIISTVIIYVIAKLFGETEGITTAFLAALI
                     GTVVYAIIYYVIGQGLIAAFIAGIVWLLVLQKLYTIGWLKSLLIAVIVWIVTSIVGWF
                     LPALTGPL"
     gene            5548..>5948
                     /locus_tag="VFC43_07485"
     CDS             5548..>5948
                     /locus_tag="VFC43_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZK31053.1"
                     /translation="MTKTKVSLQLVSMMNNALENEHAAYVQYLSHAKIVKGINAKPII
                     NRLKGIARDHRQHQRKLRHLLGDYLGAVPSMKIGKTYEADNIDKILTTNLKFEKDTID
                     NYMKTLAELNKEKSNLPYEFLKINFALERIV"
BASE COUNT         1591 a         1429 c         1476 g         1452 t
ORIGIN      
        1 ggtatatctt gagcgtcttg acaaacctgc gatatttgta aaaaaaggtt gatccgagat
       61 ccatggtgaa cgctcctctt cctgctgtgt tacatagagc agcagcaaaa accagatcag
      121 gccgtaccca aactttgccc cagtcacggt ataggtggtg atccccccgg cgtcattatc
      181 agcaagcata acgatgaggc cgggcccggc aaggaggagg tatatcttga gcgtcttgac
      241 aaacctgcga tatttgtaaa aaaaggttga tccgagatcc atggtgaacg ctccctcttc
      301 ctgctgtgtt acatagtgtt aaaccagtcc ccccttaccg tgaatttccc aaacatcagg
      361 atattcacca ttatggcagg caactgcagg taaatcggca taaacgttta aataaattgt
      421 gagatgctta ccacaattac aggccggtta cccatttctc ctcctctgag gaaggtatac
      481 gggaaatcct gccctccctt gccccatacg ttcgggaacc tcaaaaaatg aatctgaccc
      541 ccggggaaaa ttaccgatgg cacaggaatg gaaatcggga attaaaaagg aggataagaa
      601 tagttaattc tgtacgatga tcgtgccttt cattgtggga tgaattgtac agtaatagtt
      661 ataggtcccg gtctggttga aggtgaactg gtatgatgcc ccgtttgcaa gcgattctga
      721 cgtgaacgca actaccgacc ccgggtctga agcgatcttg tgcggtgcac cgtcctggtt
      781 catccaggta acagtggttc cggtctttat tgtcagtatt gccggactga acgcaaagtt
      841 tttgatcgca attgagttca atcccgccac aggagtcggg gttgtcggtg tggatggtgc
      901 cgggctttga ttggaagtac agccggaaaa cgaacaggca aggaccagca ggatcgcaca
      961 tgccgatgag aaaattacac gggaatgaga aatcatagtt tcccataaag cggtgagtag
     1021 tgaaaaatgt tcctgccggt atcaatagtg cccgaattat tgaatacgaa tcctgacggt
     1081 gaaaaagggt gtcagggtat tcatgtacca ggagacgatg ggaccgcatc cctttttcgg
     1141 actcttttgt tccagtactt gtagcactca aaccagacaa cactcaaccc gcctgctatg
     1201 acgcacaaaa taaggtcagc aaagggcact gaaccgaacc tgaagaggtc ctggaggaac
     1261 gggatgaaca ggacgaggat gaggcagctg atcgttcctg caaacaccca gatcatcgcc
     1321 ttattgggag tgcgtagtgt tgtcagcatg gtttcagacc atgaacggtt tgtcaggatc
     1381 aggcagaggt ttgcgatcac gatggtcgtg aacgtgaggg tcctcacttc tgcctctcca
     1441 aagcccctga gtatggcatc agcgtagatg gtaaggacca cagccaggac gacaaatccc
     1501 tgcaggagac tgagtgcgag cgtctttttg gtaaaaagcc cctcatccct gtgccggggc
     1561 gggcggttca taacgtcagg ctcttctttt tctgattcaa acacgaccga acaggcagga
     1621 tcaataatca gctcgagaaa gacgatatgc acaggcaaca ggaccagcgg catgttaaag
     1681 agcacgggca taagcgacat tcctgcgata gggacatgga cggagaagat gtacgccatc
     1741 gcttttttga ggttatcaaa tatcctccgt cccagccgta ctgcagatac aatagacgtg
     1801 aagttgtcat ccagcaggac aagagaagaa gattcgcggg caacatcggt tccccgtccc
     1861 cccatggcaa tcccgatatc tgcggatttc aatgccgggg catcatttac cccgtcgccg
     1921 gtcatggcaa caacctcgcc gttggccttg agggcattca caatccgcag tttctgttcc
     1981 ggcactgccc gtgcaaagag ggtcgcggtc ctgatacgtt ccttaagttc tgcttcgctc
     2041 atctgttcaa gttcactacc cgtgatacac tggtcggtat gggcaagacc gatctgccgg
     2101 ccgatattct gggcagtaag gggataatcc ccggtgatca tgatcacccg catcccggca
     2161 gtatagcatt cacgcactgc atctgcaacc agcggacgga ccgggtctgc aaatccgaca
     2221 agtccaagga aggtaaacgt gaaatcgtgt tgcccgtccg gcagttcagg ttttgtgaac
     2281 aatgcctttg ctacgccgag gatccggagc ccctgcactg ccatggagtc aatatctgtg
     2341 gcaagtgcct gccgtttcgc ctcatcgaaa tggcacaggt ccgcgatggc ttcaggagcc
     2401 ccttttgcag cgataacaaa ctcattaccg gctggagact gccatacgtt tgacattgca
     2461 aggagttcgg gagagagcgg gtattctgtt atgagctccc agccggtatg gatgtgttcg
     2521 gttgtcccga attccccgtc ccgcagatgg aacaatgctt tttccatggg atcgaaggga
     2581 tcttttttac aggccagaat ggcaaattcg gcaacctcat ggcatatttc cgggactgaa
     2641 ttttttcctg cggcatcatg gacacacagc tgccctgcag caaaaaacga atggacggtc
     2701 atcttgttct gggtgagggt accggtcttg tccacgcaca gcacggtcgc tgaaccgagc
     2761 gtttctatcg cgggaacctg gcgggtcagc acatttttct tggagatcct ccaggcaccc
     2821 agtgcaagga agatggtcag caccacggga aattcttccg gcaggattgc catggcaaga
     2881 gtgatccccg caaggaagcc ctcggtccag ttaccccggc tcaggccgta cacgacaaca
     2941 ataacagcac acaggaagat gccgacgatg gcaattgtcc ggacaatccg ggatatctcg
     3001 cttttgagcc gggtttcccc ccgctccacc gtctgcagga cggtaccgat cttccccatc
     3061 tcggtccgtg taccggtcga ttttacctct gccaggccct ggccctgaac gaccattgta
     3121 ccggaataga caaacggctg gtcatcacca ccgggtcgtt cagcatgaag cccatcggtc
     3181 cagggtactt tccggaccgg gaccgattct ccggtcagga gtgattcatc cactgtcacg
     3241 ttatttgagg acaggaggac accatcagcc gggaccctgt cgccttcgac aaggacgagc
     3301 atgtccccgg taaccacttc gcggccggca atccttttct gttcaccgtc ccggattacc
     3361 agcgcacggg gacttgagag attccgcagt gcttccagtg cccgttcagt cttctgctcc
     3421 tggtagaccg taatcccgat gatgacaacg acaaagctca tcagcatgat gccctcggta
     3481 agatcaccga ggataaaata gatcaggccg ctggcgacca gcagtatgaa catcggttcg
     3541 tggattactt cgaaaataat ccagaaaata ccgcgtttct ttgactcggg gagctcgtta
     3601 tacccctctg tcctgatctt ttcagctgct tcctcacaag aaagaccttt gactgagtcc
     3661 aggtccagaa atggcattat gacggttctc ccaaaaaggc cttcagagca tgcattttat
     3721 acataagatg ttaccatacg tgatattttc ttttgcccgg ctgttttcgg aggaactgat
     3781 tttcatgctt aaacccggaa gtttaccaac tgtgatgaaa tacgtttttt gatggaattc
     3841 agcttctcct gcaacagaag gatagctaaa tggattttac aaaattgctc aggttacttt
     3901 gatatgggac gactgtgtaa aggagaacgt attcaatcag ataaggagga aagaacatga
     3961 agaaaggatt tgcagcaaat cttgaagaag aaacgaggaa gaataccgat ttccgcaggg
     4021 taatgtacac aggaaaacac agtcagctcg tactgatgtg cctgaaacca aatgaggaaa
     4081 tcggtgagga aacccacgat gatgttgacc agttcttccg gttcgaagag ggtgaaggtg
     4141 ctgtcatcat tgacggcgtc aagcacgtgg tgaaggatgg aagcggtgta gtcgtcccgt
     4201 ccggcgcaaa acataatgtc gtgaacacat caaaggtaaa aaacctcaaa ctgtacacga
     4261 tctactcacc acccgaacac caggacaagg ttgtgcggaa gacaaagcag gaagccatgg
     4321 caaaagaaga gcattacgac gggaaaccaa cggagaagta acggctgata atctggtgct
     4381 ctctcatttt ttcctgcatc cggtacccgc aggtatcacc ccgcacaatg aaattcacca
     4441 tgttttccgg gattgctctt atcgggctct tgtctataac cttcaacggt aagacattgc
     4501 aggatcccgt aagcggacga atcctctcaa actatccgcc aatccgatag tattaattgt
     4561 atcaaagaag aggaatgcac tatggcatca attgttttta ccctcttgtc atttgttatc
     4621 gcgctgatca tctcaacggt gataatttat gtcattgcaa agttgtttgg tgaaacagaa
     4681 ggaataacca cagcatttct cgctgccctc atcggtacgg tagtatacgc tatcatatat
     4741 tatgtgatag gccagggcct gatagctgca ttcatagcgg gtattgtctg gctgctggta
     4801 ttgcagaaac tgtacaccat cggctggctc aagtccctgt tgatcgctgt tattgtctgg
     4861 attgtgacat ccattgtcgg atggttcctg ccggcactga ccggtcccct gtgaacctgc
     4921 gacctgtcaa cctttttagt aaaaaggggg ataataaatt tatttacccc ccagacccgg
     4981 cctgccacta ccctgtgagt gggatgagag tatccgctcg cccggtctct ctatacaatc
     5041 ggctctgaaa acctcttcca ggatccggag ggatacaggg aaatgttttg aaaggttacg
     5101 tctcgtcctg gattttttat attgttggga gtgatagatt tgtccgaaac acctggttca
     5161 tcagtggtaa aaattatcga attgatcgga agtttcgccc aatgggtggg aagaggcagt
     5221 agacaatgcc gttaaggaag cagcgaaaat gtgaggaata tcaaaggtct agatcaaaaa
     5281 caaagatgct cagctaaagt gcagaatgat agtatcctgg aataccgtgc cgctgttaaa
     5341 attgcatttc ttgtagaaag tgagcaataa tcttattttt ggatttttta ggatttcagc
     5401 ataccggaaa tcatccccaa taaaaaatta tactatcccg atttttctat acctaaacag
     5461 caaatcggtg attttatgat gttctctttt ttgtaattcg tgtgagagta taaatatggc
     5521 attatgccag tagtgcaatg atgtgatatg acgaaaacaa aagtttctct acaacttgtc
     5581 agtatgatga acaatgctct tgaaaatgag catgctgcat atgtgcagta cctgagccat
     5641 gcaaaaattg tgaagggtat caacgcaaaa cctatcatta accggctgaa gggtattgcc
     5701 agagaccacc ggcagcacca gaggaagtta cggcacctcc tcggggatta tttgggggct
     5761 gttccctcca tgaagatagg aaagacgtac gaagctgaca atattgacaa gatcctgacg
     5821 acgaatctta aattcgagaa agacactatc gacaattaca tgaaaacact ggcagagctc
     5881 aacaaagaaa agagtaatct tccgtatgaa ttcctaaaaa tcaattttgc tcttgaacgt
     5941 attgtcag
//
LOCUS       DASRLV010000196         5745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_561742,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000196 DASRLV010000000
VERSION     DASRLV010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561742"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /locus_tag="VFC43_07490"
     CDS             <1..139
                     /locus_tag="VFC43_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HZK31054.1"
                     /translation="RMTAQIVCSPDEVKIGMRVKSVFRRIATDGESGIIHYGTKFVPA
                     E"
     gene            complement(506..1849)
                     /locus_tag="VFC43_07495"
     CDS             complement(506..1849)
                     /locus_tag="VFC43_07495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZK31055.1"
                     /translation="MISVLLVEDDPDVLELTRLFLERDLELNIDVCSSVKEALRKLNN
                     KIYNVIVSDYLMPEINGIQLLKTLKFQGIETPFIVFTGQGGEDIAIEALNSGATFYLQ
                     KSENPRFQFAKLRKMIHEAALKQKTEETYREQNISVVIPAYNEGLLIGPTLASVPDYI
                     FRIYAVDDASTDRTPEIIREYAEKDIRIIHIRHEINKGVGASIATGYREALKEGMDIA
                     VVMAGDNQMDPAYLPQFLDPILNHQADYAVGNRLQTTEFRKGMPKYRLFGNALLTLLT
                     KIASGYWQLMDPQNGYTAISRRALERLDLSKIYPRYGYCNDLLVKLNAYGFKAINVNH
                     KARYAIGEVSGIKMRTYIFRLSRLLFKDFLWRLKEKYVILSFHPLVFFYIFGVVAILM
                     GILFGAYTIYLKLFVELELFVVRAALAILIFFLGCMFLLFAMLFDMEQERNMGWT"
     gene            complement(2127..2366)
                     /locus_tag="VFC43_07500"
     CDS             complement(2127..2366)
                     /locus_tag="VFC43_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HZK31056.1"
                     /translation="MEPHTNGTQRASVRIGLVVDVIQKEDQRSKKKTRGIVQEILTNS
                     LSHPHGIKVRLKSGQVGRVVEIILPAPSENSKDES"
     gene            complement(2444..3187)
                     /locus_tag="VFC43_07505"
     CDS             complement(2444..3187)
                     /locus_tag="VFC43_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31057.1"
                     /translation="MDRVIRTALFVFIITGLAFSSVVVYQTGVENAYRASLSSTYSYT
                     CVITTDSLLSNVTLFIPVPADMQGNSPIVERFSTRDITGIPDDWSVSLYDTGKATMVR
                     ISTPAIPPTGNTSEKNPLTFVLSSEIQSERVIDSRDPEQNSALFRPVREVRQVTCPPD
                     SSAINGTPRCYHYLTSLYADYQASPGAMVEISSSITGNNTWKIFEPRSNEYTSTITLN
                     MSGENRGWQKVDGFLKNNTGSYDAPDIIQ"
     gene            3387..4643
                     /gene="pyrC"
                     /locus_tag="VFC43_07510"
     CDS             3387..4643
                     /gene="pyrC"
                     /locus_tag="VFC43_07510"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HZK31058.1"
                     /translation="MNSPPSLVLGNVTLPSGRVLDITLAEGRVSHAGASQPSAEFIDC
                     TGLIVIPAAIDMHVHMRGGSQSKKEDWKSGSMSALAGGVTVVVDQPNTTPPINTPEHL
                     HDRIGDAQEHSFCNFAINSSVTPQTPLEKMDEAGALAYGETFFAPSSYGEGIGDTALQ
                     NAFCRIRALDGLATIHAEKVSDIPDTDLFVHNLVRSPDGEVQAVKEVKKLNTAGCRLH
                     FCHLSTKRSIDAATATVEVTPHHLLLSYEDFEKTDAFVKVNPPLRTEKEQKSLWRRWN
                     RIDVIASDHAPHTRTEKGSDFSVSPSGIPGVETMVPLLLARVVQGKISLQSLIEKTSS
                     APADLLGIPRAGFCVGDRADFALFEKIPRPVTPEMLHSKCGWTPFEGHLALFPRTVIM
                     GGTIVYKDGDFFRSEPRWFSGKGYKK"
     gene            5137..5457
                     /locus_tag="VFC43_07515"
     CDS             5137..5457
                     /locus_tag="VFC43_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HZK31059.1"
                     /translation="MPDIADALLEDRFGTIITLEVSAHAKTCVFPAGYNAWRKTIGCR
                     VTAPASEGKANHAVISLVSDTLGVPASRVSIQSGATSPQKRVLVERMNKKDLLARLLE
                     IAGP"
BASE COUNT         1541 a         1351 c         1331 g         1522 t
ORIGIN      
        1 gcgtatgacc gcacagatcg tctgtagtcc tgacgaagtc aaaattggta tgcgggtgaa
       61 aagcgtgttc cgccggatag cgaccgatgg cgagagtggt atcatccact acggaacaaa
      121 atttgtgccc gctgagtaaa ttatctcatt tttttgcagg gtttttcggg agcatgcaag
      181 cctcaaaccc gagttttgta gcaggcatta caacgtcagc gaatggggat gtcctagtga
      241 tgggggcgat acccaaaaga gcgtcagaat tttcataaat aatttcctat aaaaatcgaa
      301 aaaattaggt atgatgaggg attatcatct ctcttcttca gttacgatga acgccgacgc
      361 cctcgaaggg acgctcccgc ggcggatcag taactgaaaa tacaattaac ctcctgtccc
      421 gccgtgccta aagagaggcc caaggttcat atctgtttct ccaggaccgg tcaaaaaccc
      481 catatatttt cttcttccct tgaaatcagg tccatcccat attcctttcc tgttccatat
      541 caaagagcat cgcaaagagc aaaaacatac agcccaggaa gaaaatgagg attgcaagag
      601 cagcccggac aacaaataat tccaattcca cgaacaattt aaggtatatg gtataggccc
      661 caaaaagtat acccattaaa attgcgacca ccccaaaaat gtaaaaaaat accagaggat
      721 ggaaactcag aatgacgtac ttttccttaa gtctccagag aaaatcctta aaaagaagcc
      781 gggacagccg gaatatgtag gtacgcattt tgatcccgga aacctcaccg attgcatacc
      841 gcgccttatg attgacattt atcgccttga aaccgtacgc gtttaatttt accaggagat
      901 cgttacagta cccatatctc ggatagattt tgctaagatc gaggcgttcg agggctctcc
      961 gtgatatcgc agtgtaacca ttctgcgggt ccatcagctg ccagtatccg gatgcaatct
     1021 tggtgagaag agtcaggagg gcattgccaa aaagcctgta ctttggcatc cctttccgaa
     1081 attcagtagt ctggaggcgg tttccgacag cgtaatcggc ctgatggttg agaatcggat
     1141 cgagaaactg cgggagatac gcggggtcca tctggttatc tcctgccatg acaacggcga
     1201 tatccatacc ttctttcaag gcttcccgat atccggttgc tatggatgcc cccacacctt
     1261 tatttatttc atggcggata tggataatcc tgatatcttt ttcagcatat tccctgataa
     1321 tttccggtgt ccggtcggtg gatgcatcgt caacagcgta tatcctgaag atgtaatcag
     1381 gtactgaagc aagagtaggg ccgataagca gtccttcgtt gtacgcaggg attaccacac
     1441 tgatattttg ttcacgatag gtttcttcgg tcttctgctt aagggcagct tcgtgaatca
     1501 tcttgcgtaa ttttgcaaac tggaaccgtg gattttcact cttctggaga taaaaagttg
     1561 ctccggagtt taatgcctcg atggcaatat cttctccccc ctggccggta aaaacaataa
     1621 acggggtttc aatgccctga aatttcagcg tttttaacag ctggatcccg tttatttccg
     1681 gcatcaggta atcagaaaca atcacgttat agatcttatt atttaatttc cgcagagctt
     1741 ctttgaccga ggaacagaca tcgatattga gttcaagatc cctctcgaga aaaagtctgg
     1801 taagctcaag aacatcagga tcatcttcaa cgagtaatac ggatatcatg gttatttcct
     1861 gcgaaaaata taatctgttt agatttttag gcgatcatta aattataatc tttccctgat
     1921 tcttacgatt cgatagtaat taattatttt ttttacctta aacaattatt cctctggaac
     1981 agggtgtctt tttttgtatt tttgtctttt cctgattccg gggtctttat tcagcaatca
     2041 cgttcgttgc ataccacaaa acccgatccc gtacaaacca tggatttcat ccttcaaaaa
     2101 caaaaaagac catggtggaa gggaaactat gattcatcct ttgaattttc agagggagca
     2161 ggcagtataa tctctacgac ccttcccacc tgaccactct tcagccgtac tttgatcccg
     2221 tggggatgtg ataaggagtt cgtcaggatc tcctgcacga tgccccgtgt cttttttttg
     2281 ctgcgctggt cttctttttg tatcacgtcg acaacaagac caatcctgac cgaggccctc
     2341 tgcgttccat ttgtgtgtgg ttccatggtt ttatttttcc taaaaaggat atgaatcgac
     2401 ccgaatattt tttagctgct gatgaaggga gaattcttca cgatcattgt ataatatcgg
     2461 gagcatcata tgacccggta ttgttcttca ggaatccatc aaccttctgc catccccggt
     2521 tctctcctga catattcagg gtgatcgtcg aagtgtattc atttgaccgt ggctcgaaaa
     2581 ttttccatgt gttgttgccg gtaatagacg aactgatctc aaccatcgca ccgggtgatg
     2641 cctggtaatc tgcatacagt gacgtgaggt aatgatagca gcggggagtc ccgtttatcg
     2701 ctgaactgtc aggagggcag gtaacctgcc tgacttcccg gaccgggcgg aacagggcac
     2761 tgttttgttc cgggtcacgg ctgtctatca ccctctcgga ttgtatttct gatgacagca
     2821 cgaaggtgag aggatttttt tcagaagtat tccccgttgg cggaatcgct ggtgtgctaa
     2881 tcctgaccat tgtcgccttg ccggtatcat acagggatac tgaccagtca tcggggatcc
     2941 cggtgatatc acgggtactg aaccgttcca ctatgggaga atttccctgc atatcggcag
     3001 gtaccggaat gaaaagcgtt acattggaga gcagcgaatc ggtcgtaata acgcaggtgt
     3061 aggaatacgt actggataat gaggcccggt acgcattttc cacaccagtc tggtacacaa
     3121 caacggatga aaacgccagt cccgtgatta tgaacacaaa cagggcggtt ctgataactc
     3181 tgtccaagat acaacccctc cttgtttttg tcttatcgta aaggaaaccg ctttttatta
     3241 agttttcaga tacagtttcc cttgaagact attggctttt gatgattgaa tagctgtcgc
     3301 tgataaccgg gtcggttttt tatgccgcga tcaggtgctc tgaatggatc accatacctt
     3361 tatggttatg agaaaaattg agtagtatga attcaccccc gtccctcgtt ctcggcaacg
     3421 tgacccttcc ctcagggcgc gttctggaca taacgttagc cgaaggacgg gtctctcacg
     3481 cgggggcaag ccagccctca gcagagttca tcgattgtac cggcctcatt gtcattccgg
     3541 cggcaatcga catgcacgta catatgcggg ggggcagcca gtcaaaaaaa gaggactgga
     3601 agagcggcag catgagtgca cttgcgggag gagtgacagt tgtggtagac cagccaaata
     3661 ctacgcctcc tatcaatacg ccggaacacc tgcatgatcg tatcggtgat gcacaggaac
     3721 attcattctg caattttgcc ataaacagca gtgttactcc ccagacaccc cttgagaaga
     3781 tggatgaagc aggtgcactg gcatacggtg agactttttt tgcaccatcg agttatggag
     3841 agggaattgg cgataccgca ttgcaaaacg cattttgccg gatccgtgca ctggatggac
     3901 ttgccaccat tcatgccgaa aaggtctctg atattccgga taccgatctt tttgttcata
     3961 acctggttcg atctccggac ggtgaggtgc aggcagtaaa ggaagttaaa aaactgaaca
     4021 cggcaggatg ccgcctccac ttctgtcatc tcagcacgaa gcgatccatc gatgctgcga
     4081 ccgcaacggt tgaagtaacc cctcaccacc tgctcctttc atatgaagat tttgaaaaaa
     4141 ccgatgcttt tgtaaaagtt aaccccccgc ttcgcacgga aaaggagcaa aagagcctct
     4201 ggagaagatg gaacaggata gatgtcatag cctcagatca tgccccgcat acccggacag
     4261 aaaaagggtc agatttttcc gtatctccca gtggcatccc gggtgttgaa accatggtac
     4321 cgcttctgct tgcccgggtt gttcagggta aaatttcctt acaatcgctc atagaaaaaa
     4381 cctcctctgc accagcagac cttctcggca ttccccgggc cggcttttgc gtaggagacc
     4441 gtgcggattt tgcccttttt gaaaaaattc cccgtcctgt cactcccgaa atgctccata
     4501 gtaaatgtgg ctggacaccc tttgagggtc atttagccct ctttccccgg acggtgatca
     4561 tggggggtac tatcgtatat aaagacggcg actttttcag gtcagaaccc cgctggtttt
     4621 caggaaaggg atataaaaaa taactgctca gtagaaaacc tttttttttg gtaaaataat
     4681 gccccccctc ttgcgccaag gagtcccccc gagggggacg ggcgcattgc gataacccga
     4741 gtccgatagc aggtaataca tcgttatcgc aatggggata cacaagtggc gggtgaaaaa
     4801 gcacaaaaga acgtctgatt ttccaaaata atttctccaa agtgaaaaat aaaaaaaacc
     4861 gtgaacgaaa caataattga acaccggctt ttgttctcag tacctcattc caaagtgcta
     4921 aataccaaaa aaaagacata tatgctgccg ataaagcgca tccataggtc cccgggtgat
     4981 ttattgacaa gcgtctggaa gtgatccatg ggacaacccg gatgcgcgac aggcagcgcc
     5041 cggcaagggt taaaacacag gtgaagaacg gcagcacgcc actggtgatc catgtgcgtt
     5101 attgccgggc aacattcctt taaaaagagt ccccccatgc cagacatcgc tgatgctctt
     5161 ttggaagacc ggtttggcac gattattacc cttgaggttt ctgcacatgc aaagacctgt
     5221 gtatttcctg caggttacaa tgcatggcga aagaccatag gctgtcgtgt aactgctccg
     5281 gcatcagagg ggaaagccaa ccatgcggta atttcccttg tatcggacac gcttggtgtt
     5341 cctgcttctc gggtgagcat ccagtccggt gcaacctccc cgcaaaaacg tgtgctggta
     5401 gaaagaatga acaaaaaaga tctccttgca agacttttag aaattgcagg accctaatct
     5461 tttcagataa ataaaaaaac tcatgcagtt ttttaacgag aaaattgctg tgttgaatac
     5521 ttttttattg aaataatgct ccctcttgcg ctaattagtc cccgttgggg cacgggcgca
     5581 ttacgataaa ccgggtttaa tagcaggtaa tacatcgtca tcgcaagggg atgccccgtt
     5641 ggcggggggg gaaaagcccc aaagaacatc agaattttca gggggagttc ccatgaaaat
     5701 cgaaaaattc aggtataatg agtggattat catcgctatt tttca
//
LOCUS       DASRLV010000197         9909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1204746,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000197 DASRLV010000000
VERSION     DASRLV010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 9909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9909
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204746"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..823
                     /locus_tag="VFC43_07520"
     CDS             80..823
                     /locus_tag="VFC43_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HZK31060.1"
                     /translation="MKVTFLGTNGWYDTKTGNTCSVLLQAEEYDIILDAGNGLAKADR
                     YITQDKPVYLFISHFHLDHIAGLHTLVKFRFSKGLKIFGQPGTTAILNTFVAEPFTVP
                     IAQLPFTVEMIDLAEGDHTLPFPLTCLPLVHPVPCFGFRIVIDGRVIAYCTDTGVCNN
                     AITLGQNADLLITECGLKPGQESPGWPHLNPENAVHIANEAHAKRLALVHFGAEVYRT
                     LKDRIEVYNQYEKQCPGLVVATDDLTIEI"
     gene            complement(833..1408)
                     /locus_tag="VFC43_07525"
     CDS             complement(833..1408)
                     /locus_tag="VFC43_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HZK31061.1"
                     /translation="MKVVAFNGSARKDGNTAILVNVVFKELKKAGIKTELVQLAGKKI
                     RGCTACGKCFENQDKQCAVKGDVANDCIGKMLEADGIILASPTYFADVSTELKALIDR
                     SGFVAKANQDMFKRKVGAGVIAVRRGGAIHAFDTINHFFFINQMIVPGSSYWNIGIGL
                     APGDVNGDDEGISIMTTLGANMAWLMKRIQE"
     gene            1530..3128
                     /locus_tag="VFC43_07530"
     CDS             1530..3128
                     /locus_tag="VFC43_07530"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZK31062.1"
                     /translation="MNTRMQFSDFHLSREIGKAIEDMGFEEPTPIQALAIPLIQAGRD
                     VTAQAQTGTGKTAAFGIPIIGKIDATHRTVQAIVLCPTRELAIQIAEEFSELLSHLPR
                     INVLPVYGGQPIDRQLRALHSGVNIVIGTPGRVMDHLRRHTLSLDEVSTVVLDEADQM
                     LDMGFRDDIELILKKIPQKRQTLLFSATLPQPIIDISKRFMNKPEFVKVEYADLIVPQ
                     IEQSYIEVKEREKLDVLCRLIDIADPHLSIIFCNTKRRAEELAGKIKARGYRAEELHG
                     DMKQSQRDRVMGGFRKGSIEILVATDVAARGIDVEDVDMIINFDVPQDVEYYVHRIGR
                     TGRAGKSGKAVTFVSPKDFTKLREIQHYAKIQIPRIPVPTLSDVAETRTRGVLEKVRD
                     TIKAGGMDTYVRIIEQETESDLSTIDIAAALLKIQMEQGNEPEVEKRDEMDFGDTGAE
                     TGMVRFFLSVGRMHNVAPKDIVGAIAGETGIPGKAIGAIRILDSYSFVEIPREHAHEV
                     YTIMKERTIRNQPTGIEPASGKRA"
     gene            3271..3768
                     /locus_tag="VFC43_07535"
     CDS             3271..3768
                     /locus_tag="VFC43_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31063.1"
                     /translation="MKKILVITTALVLCTVLILTAGCTQPATPTATPTPTAAATTIAA
                     TPTASSTVSSLTPGPTQTLPETWNVEIEVKSNGESINPLIITTVRGGKGLIFIQQIDV
                     KVTRSDGVVETGTITKPLTVGKTVELTSTTAPGYRDRAEVWATTPQGEKVKIFDAYVP
                     FRTYN"
     gene            complement(3885..4334)
                     /locus_tag="VFC43_07540"
     CDS             complement(3885..4334)
                     /locus_tag="VFC43_07540"
                     /inference="COORDINATES: protein motif:HMM:NF021612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase-like protein"
                     /protein_id="HZK31064.1"
                     /translation="MPYEDLLLIIIISLVIGFILGFFYVKSQITAIEKMYRVELECWK
                     VGASEEIRKDAVNRSRSTLKGKIAEQIAPLLPEFLYNPADARFIGSPVDYIIFDGLTE
                     VSDDKKSSIRIIFMDVKKGSASLTRTQRVIKGAIEEKAVSWETLRIK"
     gene            complement(4354..5265)
                     /locus_tag="VFC43_07545"
     CDS             complement(4354..5265)
                     /locus_tag="VFC43_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HZK31065.1"
                     /translation="MEPVTEILRKIEINSDRDRYVLTLNSEDFSFLYPGLVRYTLSLK
                     EGEKTISVFRTNSYEYSPLVPLTAENVAKRTADEWEQEIHNSPMDFILKHGQVSLQRA
                     APADIDLVVIQGSPRADGNCSILAGWAVEAAHDLGRQSRVIYPHDLDIRCCIGCYQCY
                     NTGSCVFDDDMRSIIDDIRSASLLVVCSPVYTNTVPAGLKSIIDRCQAYHAERVLSGG
                     RTGQHGLIFSVAGRKGEGNFTCVTRVITAFLRNLGITPRGELLIDSVDAVRDIRLIHG
                     LEQTVREKVKQCLVFENSAENLLKQDI"
     gene            5420..6142
                     /locus_tag="VFC43_07550"
     CDS             5420..6142
                     /locus_tag="VFC43_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HZK31066.1"
                     /translation="MIPVSLIPIPLRIAALTQERNSLLEYPVERLASVIREIGFQCSS
                     CAKCCTRAFNGHVFLLDRDVTAVKTIDPAAIEPAPAPEFCDQNGIFYVSGYAIRIQDD
                     DGGSCWFLENGKCRIYERRFTICRIYPYMLHREADEKGCVDWRQFCGLDQHGEYNAAI
                     PENESLDLARETKEYENAFLTQEIRFLEFMQDYFSRHRLRHVQKVYDDGMRRFNRDEE
                     ITVMVYYDGLLERHRIKKQTEP"
     gene            complement(6205..7032)
                     /locus_tag="VFC43_07555"
     CDS             complement(6205..7032)
                     /locus_tag="VFC43_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HZK31067.1"
                     /translation="MAKKLYPFLISVPHGGTAVPESVRPLLQMRDEDLRYYCDPFTRI
                     VFGYKNRVAAYIDSPVSRMVVDLNRPPLPLPLRDPDGIIKVKTVDGREVYRPGQVPDM
                     HHIHSLLKEHYFPYHQRIDELIDHEPVRIAFDCHSMLPYGSKDQKDAGKKRPLICLSN
                     NGDLQGKAKKGSITTCSGEWITGLAGIFREEFSFDKEVAINNPFSGGFISNAHYWHKG
                     IPWIQIELNRALYEPDASHALSQKETRARVLWLKEKVWTVLTTFWNRSGNLRDPHTR"
     gene            complement(7210..7365)
                     /locus_tag="VFC43_07560"
     CDS             complement(7210..7365)
                     /locus_tag="VFC43_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31068.1"
                     /translation="MEKPMVKENQKIVRIRQKIKEIFDCDCSMNDQMLMKRYAITDNG
                     ELIIYVK"
     gene            7656..8105
                     /locus_tag="VFC43_07565"
     CDS             7656..8105
                     /locus_tag="VFC43_07565"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZK31069.1"
                     /translation="MAPAFCLPDAEGVQVCLNSFKGKWIVLYFYPRDNTPGCTLEAMQ
                     FNAALEKFADLGAQVIGVSADSSESHQKFAAKHNLSILLLSDTEHAVLKEYDSWKPKT
                     MFGKEFLGTQRDTFLIDPEGKIISVWRRVSPKGHAEEVKAVLLEKKK"
     gene            8559..9869
                     /locus_tag="VFC43_07570"
     CDS             8559..9869
                     /locus_tag="VFC43_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HZK31070.1"
                     /translation="MSYLLEIVIILILIVINGIFAMAEFAIVSVKKTRLQQRADAGDT
                     GAVAALEIVNEPTQFLSTVQIGITLVGIFAGAFGGATVARELAVYFTEFPALAPYSDL
                     LSIVIVVLVITYLTLIFGELVPKRLALNNAENIASAVAKPMRLLSTITSPLVIILSHS
                     TEAMLRVLQFRKITEPPVTEEEIKIMLAESTEAGVFEKAELSMVEGVFDLGDRRVESL
                     MTHRSDVVALDLNDPADENLRKMIQSGRSNFPVYEGNLDTIVGMVSVKDLLARMVDGG
                     TPDIKASVTKPIFAPQALPVLKLLESFKGTGVHIALVTDEYGSIQGVITLHDILEAIV
                     GDVRTLGEPVEIPVVVREDGSWLISGDTPVEDIKKILSVDSFPGEELGHYRTFAGLIL
                     FILERIPKTGDHFEAKGLRYEVVDMDGNRIDKVLVTRVPTEPPA"
BASE COUNT         2652 a         2413 c         2265 g         2579 t
ORIGIN      
        1 atataataat ttgtgcacaa aaaagagggg atcgcaaata actctacctt tttgacctgt
       61 aaaacataat ccggtaacca tgaaggttac cttccttggt acaaacggct ggtacgatac
      121 gaaaaccgga aatacctgtt cggtcctgtt acaggcagaa gaatatgaca tcattcttga
      181 tgcaggaaat ggtctggcta aagcggaccg ctatatcacc caggataaac ctgtatatct
      241 tttcatcagc cattttcacc ttgaccacat agccgggctg catactctcg tcaaattcag
      301 attttccaaa ggcctgaaaa ttttcgggca gccgggaacg acggcgatcc tcaatacatt
      361 tgtagctgaa ccgtttactg tcccgattgc acaactcccg ttcacggttg aaatgatcga
      421 tcttgcggag ggagatcata ccctcccgtt ccctcttacg tgcctgcctc ttgtacaccc
      481 ggtgccctgt tttggcttcc gaatagtcat tgacgggagg gttattgcct actgcacaga
      541 taccggagtc tgtaataatg caatcacact tggccagaat gcagatcttc ttattactga
      601 atgcggatta aaaccagggc aggagagtcc gggctggccc catctcaatc ctgaaaatgc
      661 cgtgcatata gcaaatgaag cacatgcaaa gcgcctggca cttgtgcatt ttggtgcaga
      721 ggtttaccgg actctgaaag atcggattga agtgtacaac caatatgaaa aacaatgccc
      781 gggcctggtt gttgccaccg atgacctgac cattgagatc tgaagggaaa aattattcct
      841 ggattctttt catcagccat gccatgtttg cacccagtgt cgtcatgatt gaaattcctt
      901 catcgtcacc attcacatct ccgggagcaa ggccaatacc gatattccag taagaagaac
      961 ccggcacaat catctggttt atgaaaaaga agtggttgat ggtatcgaat gcatgaattg
     1021 caccacctct tcgcactgct atcacgccgg caccaacttt tcgtttgaac atatcctggt
     1081 ttgccttggc aacaaaccct gatctgtcga ttaaagcctt taattctgtt gagacatccg
     1141 caaaatacgt tggtgatgca agtatgattc catctgcttc cagcattttc ccgatacagt
     1201 cattggcaac gtcaccttta accgcgcatt gcttatcctg gttctcgaaa cattttccac
     1261 aagcagtaca tccccggatc ttctttcccg ccaattgcac aagctcggtt ttaatgcccg
     1321 cttttttgag ctccttaaac accacattca caaggatcgc tgtgttcccg tccttgcgcg
     1381 cactgccatt aaatgcaaca actttcattt cgcaccacaa taacccattc tctttaaggt
     1441 aaataaaaaa atcgcagggg tatctctgaa taaaaatacc ttccccatag cgccatctta
     1501 tgaaccctta caccaaatgg tgttgcgata tgaatactcg tatgcaattt tccgactttc
     1561 acctctcgcg tgaaatcggt aaagcaatag aagatatggg ctttgaagag cccacaccca
     1621 tccaggcact cgctataccc ctcatccagg ccggtcgtga cgtgactgcc caggcacaaa
     1681 caggtacagg caaaaccgcc gcattcggca ttccgattat tgggaagatt gatgcaacac
     1741 acagaactgt acaggcaatt gtactctgcc cgactcgtga actcgcaatc cagatagcag
     1801 aagagttctc tgaactcctt tcacatctcc cgcgcattaa cgtgcttccg gtatatgggg
     1861 ggcaaccgat agaccgccag ttgcgggcac tgcattccgg tgttaatatc gtgattggca
     1921 caccggggcg ggtaatggac catctcaggc gccacactct ctcacttgac gaggtatcaa
     1981 cggttgtact tgatgaagct gatcagatgc ttgatatggg attccgtgat gatatcgaac
     2041 tgatcctcaa aaaaattccc cagaaacgcc agacactgct cttctctgcc acccttccac
     2101 aaccgatcat cgatatctcc aaacgattca tgaacaaacc cgagtttgtc aaggttgagt
     2161 acgccgatct gatcgtcccg cagatcgaac aatcgtatat tgaggttaaa gaacgcgaaa
     2221 aactcgatgt actttgccgg ctgatcgata tcgccgaccc acacctttcg attatcttct
     2281 gcaacaccaa acgcagggca gaggaactcg ccgggaagat caaggcgcgg ggttaccgtg
     2341 ccgaggaact ccatggcgac atgaaacagt cacagcgcga cagggtgatg ggcgggttcc
     2401 ggaagggttc gatcgagatc ctggtcgcaa ccgatgtcgc tgcccggggt attgatgtag
     2461 aggatgtgga tatgatcatc aacttcgacg tgcctcagga tgtagaatat tacgtccacc
     2521 gtattggtcg gactggcagg gcgggcaaaa gtggcaaagc ggtgactttc gtatcaccaa
     2581 aggatttcac gaaactccgt gaaattcagc attacgccaa aatccagatc ccccgcatac
     2641 cggtccctac actcagcgat gtggcagaga cacgcacccg tggtgtgctg gaaaaagtgc
     2701 gtgatacgat taaggcagga ggaatggaca cctatgtccg aatcattgaa caggaaactg
     2761 agagtgacct cagtaccata gatattgctg ccgccctcct caagattcag atggaacagg
     2821 gtaatgaacc cgaggtagaa aagcgtgatg agatggattt tggtgatacc ggtgctgaaa
     2881 ccggcatggt caggttcttc ctctcggtag gaaggatgca taacgttgca ccaaaagaca
     2941 ttgtcggagc gattgccggg gagacaggaa ttcccggaaa agcgataggt gcgatccgta
     3001 tcctcgacag ttattccttt gtcgaaatac cccgtgagca cgcacatgaa gtctatacaa
     3061 tcatgaagga acggacgatc cggaaccagc caaccggtat agagccggca tcaggaaaac
     3121 gggcgtaaaa acattccttt gcatttttct ttttttacat cttagaaata tacatatctc
     3181 acataggaag tgcgtttttt gctgttgttc cagggctcgc ctgcatctca tgacacaatg
     3241 ctcatatacc ctcccgggca aaactcccgt atgaaaaaga tcctggtgat taccacagcc
     3301 cttgtgctct gtacagtact tatactgaca gccggttgca cccaacctgc aaccccgacc
     3361 gcaaccccga ctcctaccgc agccgcaaca accattgctg caactccgac ggcatcatca
     3421 accgtctctt ccctgacccc cggacccacc cagactctgc ctgaaacctg gaatgtcgaa
     3481 attgaagtga agagtaatgg ggaatcgatc aatcctctca ttatcacgac agttcgtgga
     3541 ggcaaaggct tgatctttat ccagcagatt gatgttaagg taacccgctc tgacggagta
     3601 gtggaaactg gtacgatcac caaaccgctc actgttggaa agaccgttga attaaccagc
     3661 acaacggcac ccggttaccg tgaccgtgca gaagtctggg caacaactcc ccagggagag
     3721 aaagtaaaga tctttgatgc gtacgtgcct ttccggacgt acaactagat catttttaaa
     3781 tttttttcgt tgttattttt attttaagac aaaatacgca atcgtaagaa aagaattgcc
     3841 atagagaaat atattcgcgt tttatgtcag tgggttgcat tccgtcattt gatccggaga
     3901 gtctcccagc tgaccgcctt ctcctcgatc gcccctttta ttacccgttg agtccgcgta
     3961 aggctggcac tacctttttt cacatccatg aatattatcc ggatggatga tttcttatca
     4021 tctgacacct cagtcagacc atcgaagata atataatcca ccggtgagcc gataaaccgg
     4081 gcgtcagcgg gattatataa gaactcgggg agcaacgggg caatttgttc tgctatcttt
     4141 cctttgagcg tcgagcggga gcggtttacc gcatcttttc ggatctcttc ggatgccccg
     4201 accttccagc actccagttc cacacggtac attttttcaa ttgcggtgat ctggcttttg
     4261 acgtaaaaaa aaccaaggat gaaaccaatg acaagtgaga tgatgataat caggagcagg
     4321 tcctcataag gcatactgaa gcgtagggtg ttgtcatata tcttgtttga gcaggttttc
     4381 cgctgaattt tcaaaaacca ggcattgttt cactttttcc cgtacggtct gttccagccc
     4441 gtgtattaaa cggatatccc tgaccgcatc cacgctgtct atcagcaatt cccctcttgg
     4501 tgtaattccg agatttcgca aaaatgcagt aataacccgt gtaacacagg taaaattccc
     4561 ctctcctttt cttcctgcaa cagaaaaaat caatccgtgt tgcccggttc tcccgccgga
     4621 cagtaccctc tccgcatggt atgcctggca tcggtcgatg atcgatttga gacccgcggg
     4681 gacagtattg gtgtaaacag gagaacatac aacaaggagc gaagcgctcc tgatatcatc
     4741 aatgatgctt ctcatatcat catcaaagac gcaggaaccc gtattgtagc actgataaca
     4801 ccctatgcaa caccgtatgt ccaggtcatg aggataaata acgcgtgact gcctgccaag
     4861 gtcatgtgct gcttcaaccg cccacccggc taggatactg cagttaccat ctgcccgcgg
     4921 gcttccctgg ataacaacaa gatcaatatc tgcgggagca gcacgttgca acgatacctg
     4981 gccatgctta agaataaaat ccatggggct attatggatt tcctgttccc attcatcggc
     5041 agtccgcttt gcaacatttt ctgccgtaag agggacaagg ggggaatatt catacgagtt
     5101 agtacggaac acagaaatcg ttttctcccc ttccttaagg gaaagtgtat acctgactaa
     5161 tcccggatag aggaaagaaa aatcttctga attcagggtc agaacgtacc ggtccctgtc
     5221 agaattaatc tcaattttgc gaagaatttc cgtaaccggt tccatcactg ttactatcaa
     5281 tcaccgatga caataaggct gtggtaaagc gtgtatgctg atatgaaaat caaaatggat
     5341 ccctttggga gaacttcatt ttatcatgct cacaaaagca cataaggtgt gccatcacat
     5401 tttccggtaa atctggaagg tgatccctgt ttccctgatt cctatccctt tgcgaatcgc
     5461 ggcactcacc caggaacgta acagtctcct ggaatatccc gtagaacgtc ttgcttctgt
     5521 aatccgggag ataggatttc agtgttcttc atgtgcaaaa tgctgtacca gggcattcaa
     5581 cggacacgtg ttcctgcttg atcgcgatgt aaccgctgta aaaacaattg atcctgctgc
     5641 tatcgaacct gcgcctgcac ccgaattctg cgaccagaac ggtattttct acgtctccgg
     5701 gtatgcaatt cggatacagg atgatgatgg cggatcctgc tggttcctgg aaaacggaaa
     5761 gtgccggatt tacgaacgcc gttttaccat atgccgcatt tatccctaca tgcttcaccg
     5821 tgaagctgat gaaaaagggt gtgttgactg gcggcaattc tgtgggcttg accagcacgg
     5881 ggagtataat gcagccattc ctgaaaatga gagcctcgat ctcgcccgtg aaaccaaaga
     5941 atacgaaaat gcctttctta cccaggagat ccggtttctt gaatttatgc aggattattt
     6001 ctcccgtcac cggctccgcc atgtccagaa agtctacgat gacgggatgc ggcggttcaa
     6061 ccgtgatgag gaaataaccg taatggtata ttatgacggt cttcttgagc ggcaccggat
     6121 aaaaaaacag acagaaccgt aatccaaaga aattacctgg cagtcttttt caaaaggatg
     6181 ttgtctccat tctcatcatt gctcctacct tgtatgagga tccctgaggt taccggaccg
     6241 gttccagaag gtggttaaaa ctgtccagac cttctctttc agccagagta cacgagcccg
     6301 tgtctctttt tgagagagtg cgtgtgaagc atcaggttca tacagggcac ggttcagttc
     6361 aatctggatc caggggattc ccttgtgcca gtaatgtgcg ttagagataa aaccaccgga
     6421 gaaaggattg ttgattgcca cttctttatc aaatgagaac tcttccctga aaatacccgc
     6481 aagtcctgta atccactctc cagaacacgt agtaatactg cccttttttg ccttaccctg
     6541 cagatcgcca ttattactaa gacagatcag gggacgcttt ttcccggcat ccttctggtc
     6601 ttttgatccg tagggaagca tggaatgaca gtcgaaagca atcctgacag gttcgtggtc
     6661 gatgagctca tcgattcgct ggtgataggg aaagtaatgt tctttaagca gactatggat
     6721 gtggtgcata tccggcacct gcccgggccg gtacacctcc ctcccatcaa cagtctttac
     6781 tttgataatg ccatcaggat cgcggagggg aagcggcaac gggggacggt tgaggtcaac
     6841 aaccattctt gagacaggtg aatcaatata agcagcaacg cggttcttgt atccgaatac
     6901 gatgcgggta aaaggatcgc aataatacct gagatcctca tctctcatct gtaatagcgg
     6961 gcgtacgctt tcgggaactg cggtgccccc gtgggggact gatatgagaa agggatagag
     7021 tttttttgcc atactcataa aaaatcataa gccggcagat tgtgttaaca tttgggtttt
     7081 gtgatcacag aaggagatta ctacagtatc ttgcatcacc atccgggttg tgccagtaat
     7141 ttcttgtaca tatttatcct aaaaaaaact gaaatgccgt cctgtgaatt ttttcaaggc
     7201 tcgggagagc tactttacgt agataattaa ttcaccgtta tccgtaattg catagcgttt
     7261 catgagcatc tggtcgttca ttgaacagtc acaatcaaaa atttctttga ttttctgccg
     7321 tatcctgaca attttctggt tttctttcac catcggtttt tccatcaagt acgttgatga
     7381 tatcatacca gagaaaggct gtaaagtgaa tatcaactcg ttgtgggcgg ggagtgaaag
     7441 tattgaatca aagtttgata aaacccaatt cccttccttt acagagtgag agaaaaattt
     7501 tccagaggtt ctttccccgg cacttatgct acactaaaaa acccgagggg acgtgtccag
     7561 aagatatgcc ggaacaggtg cattgaagag ctgatgtgac aatccttctc gtgatataca
     7621 tggttcagtc acaagatgat ccatttattg gtaaaatggc tccggcattt tgtcttccgg
     7681 atgctgaagg tgtacaggtc tgcctgaatt cctttaaagg aaaatggata gtactgtatt
     7741 tctatccccg ggataacaca ccgggctgta cccttgaagc gatgcagttc aatgccgcac
     7801 ttgaaaaatt tgctgatctc ggtgcacagg taatcggtgt gagtgctgac tcatcggaaa
     7861 gccatcagaa atttgccgcg aaacacaatc tttccatcct tctcctgtcg gatacagaac
     7921 atgcagttct caaagaatat gattcctgga aacccaaaac gatgtttggt aaagaattct
     7981 taggtaccca gcgggatact ttccttatcg atcctgaagg aaaaattatc tcggtgtggc
     8041 gtagggtcag cccgaaagga catgccgaag aagttaaggc agtactactc gaaaaaaaga
     8101 agtgagatac tacagttaat gattgatcct aattagtgtc gtgaaaggtt tgacttatgg
     8161 ttacacggta atcactgaat acttcgtaag acaataattc tgcgcaaaga tgtttaaaag
     8221 gctttaatct ccaaacaaac cttaatttaa aaggtagaag ttgtgatatc acatttccta
     8281 tagaacggag cccaatttag gctgattttc cctcaggatc catacccctg gattccaggt
     8341 ctcaccgacg gagggtaaat gaggaggaga tcatctcaca cccctgtcag gcacatccct
     8401 gaccatccgc aggatctccc cccaaccatt atcaaagaga atactatcct taccctggta
     8461 taacccggtt ttttttgcga cccctgactt tctggtggca aaatcctctt catttcttta
     8521 aacagtttta attaccgtgc gcacaataat tctgcaaaat gtcttatctc cttgagattg
     8581 ttattattct cattctgatc gtgatcaatg gcatctttgc catggcagaa tttgccatcg
     8641 tttcggtcaa aaaaacccgc cttcagcagc gggcagatgc aggagataca ggggcagtag
     8701 cagcacttga aatcgtgaat gaacccacac aattcctttc gacagttcag atcgggatca
     8761 ctcttgtggg tatctttgcc ggtgcattcg ggggagccac ggtagcacga gaacttgcag
     8821 tttattttac agaattccct gctctggcgc cgtacagcga cttattgagc atcgttattg
     8881 tggttcttgt aatcacctat ctgaccttaa tctttggaga acttgtcccc aaacgccttg
     8941 cgctcaataa tgcagaaaac attgcctccg ccgtcgcaaa accgatgcgg cttctttcta
     9001 ccataacctc tccgttggtg attattctca gtcattcgac tgaagctatg cttcgggtct
     9061 tgcaattccg aaaaatcacc gagcctccgg taaccgaaga ggaaattaag attatgcttg
     9121 cggaaagtac tgaagccggt gtattcgaga aggccgaact gagcatggtg gaaggtgtgt
     9181 ttgaccttgg tgaccgccgc gttgaatccc tgatgacaca ccggtctgat gttgtagcgc
     9241 tcgatttgaa tgatcccgct gatgagaacc tgcggaaaat gatccaaagc ggaagatcaa
     9301 attttccggt atatgaggga aatctggaca ctattgtcgg tatggtatcc gtaaaagatc
     9361 ttctggcgag gatggtggac ggcggcactc ccgatataaa agcctctgtc acgaaaccga
     9421 tctttgcccc ccaggcactt ccggtcctga aactcctcga atcattcaag gggaccgggg
     9481 tgcatattgc tctggtcaca gacgagtatg gaagcattca gggtgtcata accctgcatg
     9541 atatccttga agcgattgtc ggcgatgtgc gtactctcgg agagccggtg gagattcctg
     9601 ttgttgtaag agaggatggc tcatggctga tcagtgggga tacacccgtt gaagatatca
     9661 agaaaatctt atctgtggat tcttttccag gagaggaact gggacattac cggacctttg
     9721 ccgggttgat cctgttcatc ctcgagcgga taccaaaaac tggggatcat ttcgaagcca
     9781 aaggactgcg ctatgaggtt gtagatatgg atggcaaccg gatcgataag gtgctggtca
     9841 cgcgagtgcc cacggaacct cctgcttaaa cgctccccta ttcaccgggt ctgcaacagc
     9901 gggaaatag
//
LOCUS       DASRLV010000198         2916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_401634,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000198 DASRLV010000000
VERSION     DASRLV010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401634"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..941)
                     /gene="purC"
                     /locus_tag="VFC43_07575"
     CDS             complement(222..941)
                     /gene="purC"
                     /locus_tag="VFC43_07575"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HZK31071.1"
                     /translation="MKSKDLLYKGKAKSVYTTDVKDRLIVEFRDDITAFDGGKKDVLE
                     NKGNYNAEVSAFFFEYLERNGVKTHFFSMLDPHHMVVRRLDMIPLEVIVRNIAAGSLV
                     RNYPFREGSPLDPPIVVIDYKDDSRHDPMINDDIILGLNLLTAAEIKKIKKVALEVNR
                     LLSDLLAAQGITLVDFKIEFGRQGKTIYLGDEISMDSMRLWDKKTGESLDKDVYRFEK
                     GDVMAIYDKVARRITTPVKKP"
     gene            complement(938..1894)
                     /gene="cofG"
                     /locus_tag="VFC43_07580"
     CDS             complement(938..1894)
                     /gene="cofG"
                     /locus_tag="VFC43_07580"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HZK31072.1"
                     /translation="MFLPLTSVCRNRCGYCCFRTPVQEGCLMAPGEVIRTLHDGAGAG
                     CTEALFTFGEHPEEETGFTAYLRKTGYTTILEYCEAMCRESIACGILPHTNAGILTYD
                     EMERLRPVNASMGLMLETTAKIPAHANSKGKDPQIRLAMIEDAGKLRIPFTTGLLLGI
                     GETMTDREESLYAIRDLHKKYGHIQEIIVQNFCPKPGTPMAHHPVPGTEEICATIQMA
                     REILPEEIAIQIPPNLLDAARLISCGVDDLGGVSPVTIDYVNPEHPWPAIEELTSIIA
                     DAQLCERLCIYPQYIRKGWYHPDLEPLITRLSQIIKHRSSGT"
     gene            1812..2015
                     /locus_tag="VFC43_07585"
     CDS             1812..2015
                     /locus_tag="VFC43_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31073.1"
                     /translation="MRQPSCTGVRKQQYPQRFRQTEVRGKNTFREKVMMRRCIAKEYS
                     SHLLKGNVLVSRGDNHQAASVLH"
     gene            complement(2051..2572)
                     /locus_tag="VFC43_07590"
     CDS             complement(2051..2572)
                     /locus_tag="VFC43_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HZK31074.1"
                     /translation="MDDLAYSVKKIPGKIVMGVQRRTSNADGRSVKDIPACWQDFFSL
                     NMASKIPDRARTPAMFAVYSDYERDWTGEYSYLIGSEVTRADRIPDGLAVVRIPAQTY
                     AVFTAAGHMPDAVLGVWASVWGTKLPRAYTFDFELYDARLTRPDKKEAEVYIAIDDAQ
                     LQGMQIICSCAPV"
     gene            2683..>2916
                     /locus_tag="VFC43_07595"
     CDS             2683..>2916
                     /locus_tag="VFC43_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HZK31075.1"
                     /translation="MCPHALIPFKLVNPKTRLSCVLSQVEREAFARAMLEDVIFAIKD
                     ANCSPVIVGTELFDSEDIQITVSDADLNQTLNAL"
BASE COUNT          661 a          729 c          706 g          820 t
ORIGIN      
        1 ttgtttttcg tcagtattga gtgcccagcc ctgtttgtgt gcatatcctt ttgcccattt
       61 gagcatctct ttttcctcct cattttcttc tgtcatgcta tctgtcttcc ccgtgagatt
      121 ctcttctcat gaatatgttt catagcagag acgttagtct ttgtgctcgg tatccgcatc
      181 cggtttacgc tcaccgtacc gttttggatt tgccgtcatg cttatggttt ctttaccggt
      241 gtggtgatcc gccgtgcaac cttgtcataa attgccatca cgtctccctt ttcgaaccgg
      301 tacacatcct tatccagtga ctcgccggtc tttttatccc agagacgcat cgagtccata
      361 ctgatttcat cgccaagata gatcgtttta ccctgcctgc caaactcaat tttgaaatca
      421 acgagtgtta tgccctgtgc ggcgagtaaa tcgcttaaaa gacggtttac ctcaagcgcc
      481 acttttttga tctttttaat ttcagcggct gtgaggaggt ttaacccgag gataatatca
      541 tcgtttatca tcgggtcgtg tcgtgaatca tccttataat cgataacaac aatgggtgga
      601 tcgagtggcg atccttcccg gaagggataa tttctgacca gtgaaccagc tgcgatattc
      661 cggacaatca cttcaagcgg gatcatatcg agccgtctca cgaccatatg atgcggatcg
      721 agcatactga agaagtgagt tttcactcca ttccgctcca gatattcaaa aaagaatgcc
      781 gaaacttcgg cattgtaatt tcccttgttc tccaggacat cttttttgcc tccgtcaaat
      841 gcggtgatat catcacggaa ttcgacaata agtctgtctt tcacatcggt ggtgtacacc
      901 gattttgcct tgcctttgta gaggagatcc ttactcttca cgttccactg ctcctgtgct
      961 taattatttg gctgagccgg gttattagag gttccagatc agggtgatac catcctttcc
     1021 tgatatactg ggggtagatg cagagccttt cgcagagttg cgcatcagca ataatcgagg
     1081 taagttcttc gattgcgggc caggggtgtt ccgggttcac ataatcgatg gtgacgggtg
     1141 aaacaccccc cagatcatcc acaccgcagg aaatgagccg ggctgcatcg agaagattgg
     1201 gggggatctg gatcgcaatt tcctccggga ggatttcgcg tgccatctgg atcgttgcgc
     1261 agatttcttc tgtgcctggt actggatgat gggccatcgg ggtgccgggt ttgggacaaa
     1321 aattctgtac aatgatctcc tggatatgcc cgtacttttt atggagatcg cggattgcat
     1381 agagcgattc ctcccggtcg gtcatggtct cgccaatgcc aagcaaaaga cccgtggtga
     1441 acggaattct gagttttccg gcgtcttcta tcattgcaag cctgatctgc gggtcctttc
     1501 ctttggaatt ggcgtgtgcg ggaatttttg ctgttgtctc cagcatcagt cccatactgg
     1561 cattcaccgg acgcaatcgt tccatctcat cataggtgag gatacctgca ttcgtgtgcg
     1621 gcaggatacc gcaggcaata ctttccctgc acatagcctc gcagtattca agaatcgtag
     1681 tatatcctgt tttccggaga tatgcggtaa aaccggtctc ctcctctggg tgctcgccaa
     1741 acgtgaagag tgcttctgtg cagccggcac ctgccccgtc atgaagtgtt cttattactt
     1801 ctccgggagc catgaggcag ccttcctgta ccggggtccg gaagcagcag tacccgcaac
     1861 ggttccggca gactgaagta aggggcaaaa acacgttccg ggaaaaggtg atgatgcggc
     1921 gctgcatagc aaaagaatac tcatcgcatc tattgaaagg taatgttctg gtaagcagag
     1981 gggataatca tcaggcagca agcgtattgc actgattaag ggtatatctg cgagtattaa
     2041 cctcttaatt ttaaacgggt gcacaagagc agattatttg cataccttgt agctgcgcat
     2101 catcaatcgc gatatacacc tccgcctctt ttttgtcagg tcgtgttaac cgggcatcgt
     2161 acagttcgaa atcaaatgta tacgcacggg gaagctttgt cccccagaca gacgcccaga
     2221 cgccaaggac agcatccggc atatgtccgg ctgcagtaaa gacggcatat gtctgggcgg
     2281 ggatcctgac aactgccagc ccgtcgggaa tcctgtcagc cctggtaacc tcactgccga
     2341 tcaggtacga gtattcgccc gtccagtcac gttcataatc agaatagact gcaaacattg
     2401 ccggtgtcct tgcgcgatcg ggtatctttg atgccatatt caatgagaaa aaatcctgcc
     2461 agcaggcagg aatatcctta acactgcgcc catctgcatt tgaagtcctg cgctggacac
     2521 ccatgacaat ttttccggga attttcttta cgctatacgc gaggtcatcc attccggaac
     2581 tatcggtaag agggatgtta aatctgcgtt ttcactatcc cggtggttcc tgctttgcgt
     2641 accagcacct ttttttcatc tcactgcata tcacggtatg aaatgtgccc ccatgccctt
     2701 atcccgttta aactggtaaa tcccaaaacc cggctctcct gcgtgcttag ccaggtggag
     2761 agggaagcgt ttgcacgtgc tatgctggag gatgtgatct tcgctataaa ggatgcaaac
     2821 tgcagcccgg tgattgtggg aactgaatta tttgacagtg aggatatcca gattacggtc
     2881 agtgacgcgg atctgaacca gacgctcaat gctctt
//
LOCUS       DASRLV010000199         3462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_712645,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000199 DASRLV010000000
VERSION     DASRLV010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712645"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VFC43_07600"
     CDS             <1..589
                     /locus_tag="VFC43_07600"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HZK31076.1"
                     /translation="AKQYHLDKGAMKGKSEGIFVTHRAVSRLNDAVVASLREQGVAAV
                     GVHPLHAAVANNARLVAFEHRHLEKMLMLGMVPVIHGDVVMDLSRGACIVSGDQLVRY
                     LAVALKVNRVGLATDVPGVLKGDEVVPEISRNMVPSLQIGNSLHTDVTGGMRGKIDEL
                     LGLASAGIGSDIFHVSRIGDFLAGTDHGGTKIKGD"
     gene            596..1693
                     /gene="fni"
                     /locus_tag="VFC43_07605"
     CDS             596..1693
                     /gene="fni"
                     /locus_tag="VFC43_07605"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HZK31077.1"
                     /translation="MGDKKRFTSSRKLDHLHICAEQSVERGDAGFGDIRFVHNALPEC
                     DMGTIDLTTRFLNHKISSPLFISAMTGGHPGTKEVNARLARAAERFGLGMGVGSQRAA
                     LEDPSLADTFSIVRDEAPHAFLVANLGAVQLRDHGFAWVERAIEMIEADAVAIHLNFL
                     QEAIQPEGDHNAVGCLDAIGELCREFKTPVIIKETGCGISEKVAKQCWGMGVSAIDIG
                     GWGGTSWAAVESVRADKSRNEPDKRLKNLGEAFSEWGIPTVVSLAEVLATGGPVIASG
                     GVRSWLDMAKGLAFGADLCGMALPLLKPAMENDEALNRTIEIIHQELTSAMFLTGSVQ
                     IADLRKARMFILGRTRQMIEKDNPARTRKYY"
     gene            1751..3088
                     /locus_tag="VFC43_07610"
     CDS             1751..3088
                     /locus_tag="VFC43_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase J family beta-CASP ribonuclease"
                     /protein_id="HZK31078.1"
                     /translation="MDIEIIAVGGYDEVGRNMTAVRCGKEIVIFDMGLRLDQVMIHED
                     AEIENMHSLDLIKIKAIPDDTLMNSVEGTVKAIVCSHGHLDHIGAIPKLAHRYNAPII
                     ATPYATELIRQQISGEQKFGVNNKLFALKAGQRYTLSQNLVLEFVHTQHSIIDTVMPV
                     LHTPQGAIVYACDFKFDRTPVIGQPPDFARLRQIGKEGVVALIVESTYIDRPGRCPSE
                     RIAKDLVRDVITSYEDDKSAIMVSTFSSHIARVKTIAECAHEIGRKPVLLGRSMEKYS
                     VTAEQMKLVSFPETTSVFGNRRTVDRTMRRMMKEGKEKFMPIITGHQGEPGSILTRIA
                     LGDTPYLLSKGDKVLFSANVIPNPMNFGQRYKIEQHLKMAGARIFEDLHVSGHAYRED
                     HYEFIHLLNPQHIIPAHGHLKMTAGYTDFASELGYTLHSTIHMMKNGQRLIIN"
     gene            3101..>3462
                     /locus_tag="VFC43_07615"
     CDS             3101..>3462
                     /locus_tag="VFC43_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HZK31079.1"
                     /translation="MSELLPYLESVAHEIDHMIDRYFVDKVGDLNKASAHLLSAGGKR
                     LRPAVVMLAADAVKRGSSEDIIQAALALELTHTFTLIHDDIMDDDTLRRGVQTVHTKW
                     DMPTGILAGDVLYARAFE"
BASE COUNT          890 a          865 c          957 g          750 t
ORIGIN      
        1 ggcaaaacag taccatctcg acaagggtgc catgaaaggt aagagtgagg gaatattcgt
       61 aacccaccgg gcagtcagca ggctgaatga tgctgttgtc gcgtccctta gagaacaggg
      121 agtagcagct gtgggtgttc acccgctcca cgctgcggta gctaataatg ccaggcttgt
      181 tgctttcgaa caccggcatc tggaaaagat gctgatgctc gggatggttc ctgtgatcca
      241 cggcgacgtg gtgatggatc tctcacgtgg agcctgcatc gtatcgggcg atcagctcgt
      301 acggtatctc gcggttgcac taaaggtcaa tcgcgtgggg ctcgccaccg atgtaccggg
      361 agtgctgaag ggagatgagg tggttccgga aatttcccgg aatatggtac catcactcca
      421 gattggcaac tcgttgcata ccgatgtcac cggtggaatg agagggaaga tcgacgaact
      481 gctcggcctt gcgtctgcgg ggatcgggtc agatatattc catgtctcgc ggatcgggga
      541 ctttcttgca ggaaccgatc atggcgggac aaaaatcaag ggggattgac cagaaatggg
      601 agataaaaaa aggttcacct cgtcgcggaa acttgatcac ctgcacatat gcgcagaaca
      661 gtcagtagaa cgtggcgatg ccgggtttgg cgatatcagg tttgtgcaca acgccctgcc
      721 ggaatgtgat atgggcacta tcgatctcac gacccggttc ctgaaccata aaatttcctc
      781 ccccctgttt atctctgcaa tgaccggggg tcatccagga acaaaagaag tgaatgcccg
      841 gcttgcccgt gcagcggaac gttttgggct tggcatgggt gtcggctcac aacgggctgc
      901 ccttgaagac ccctctcttg cagacacgtt ctcgatagtc cgtgacgagg ctccccatgc
      961 gttcctggtt gcaaaccttg gcgctgttca gcttcgtgat cacgggtttg catgggtaga
     1021 gcgggcaatt gagatgatcg aggcagatgc ggttgccata cacttaaatt tcctgcagga
     1081 agctatccag ccggaagggg accataatgc cgtcggttgc ctggacgcaa tcggagaact
     1141 ctgtcgtgag ttcaaaaccc cggtaatcat caaagagacc ggttgcggga tttccgaaaa
     1201 agtagcaaaa caatgctggg gaatgggagt cagcgcaatc gatatcggtg gctggggcgg
     1261 gacctcctgg gcagccgtag agagtgtccg tgctgataaa agcagaaatg aacctgataa
     1321 gcgactgaaa aatctcggcg aagctttcag tgaatggggg atcccgaccg tcgtaagtct
     1381 tgccgaagtg ctcgcaaccg gaggcccggt aatagcatcg ggtggcgtca ggtcatggct
     1441 cgatatggca aaaggactcg cattcggggc cgacctctgc ggaatggctc tccccctttt
     1501 aaaacccgca atggaaaacg atgaggcact taatcgcaca attgagatta tccaccagga
     1561 actgacctct gcgatgttcc tgaccggatc agtacagatc gcagatctca gaaaagcaag
     1621 gatgtttatt ctgggccgga cacggcagat gatagaaaaa gacaacccgg cacgcaccag
     1681 aaaatattat taaaaccggc aaatttttca taagtaacac actaagacga acaacaagga
     1741 gaaatacacg atggatattg agattatagc agtgggcgga tatgatgagg tcgggcgaaa
     1801 tatgaccgcc gtccgctgtg gaaaggaaat tgtcatattt gacatgggac tccgcctgga
     1861 ccaggtaatg atccacgagg atgcggaaat tgagaatatg cactcccttg acctgataaa
     1921 gatcaaggcg ataccagatg atacgctgat gaattcggtc gagggaaccg taaaagcgat
     1981 tgtctgctca cacgggcacc ttgaccatat tggcgcgatc ccaaaactgg cccaccggta
     2041 taatgcacca atcattgcaa caccctatgc aacggaactg atccggcaac agatatccgg
     2101 tgaacagaaa tttggcgtga acaacaagct cttcgccctc aaggcaggtc agagatatac
     2161 gctgtcccag aaccttgttc tggaattcgt tcacactcag cactccatca ttgatacggt
     2221 catgccggta ctgcataccc cgcagggtgc gattgtctat gcctgcgact ttaaatttga
     2281 ccggacaccc gtgatcggac agcccccgga ttttgcccgg ctccggcaga tcgggaaaga
     2341 gggcgttgtt gcattgatcg tggagagcac gtatatcgac cgtcctggtc ggtgtcccag
     2401 cgaacgcata gcaaaagatc tcgtgcggga tgtcattacc agttatgagg acgacaagag
     2461 tgcgattatg gtttccacgt tctcatcgca tattgcccgt gtaaaaacga ttgcggagtg
     2521 tgcccatgaa atcgggagaa aacccgtgct ccttggacgt tcgatggaga aatactcggt
     2581 aaccgccgaa cagatgaaac ttgtctcctt ccccgagacc accagtgtct ttgggaaccg
     2641 gcggacggtt gacaggacaa tgcgccggat gatgaaagag ggcaaagaaa agtttatgcc
     2701 tatcatcacg ggacaccagg gcgaacccgg gtcgattctt acccgcattg cccttggaga
     2761 cacaccgtac ctgctgagca agggagacaa ggtcctcttc tcggcaaatg tcataccgaa
     2821 ccctatgaat tttgggcagc ggtataagat cgaacagcat ctgaaaatgg ccggtgcccg
     2881 gatctttgag gacctgcacg tgagcggtca cgcgtatcgc gaggaccatt acgagtttat
     2941 tcacctgctg aatccccagc atatcatccc ggcccatggg cacctgaaaa tgaccgcagg
     3001 atatacggac tttgcctctg aacttggcta cactcttcat tccacgatcc acatgatgaa
     3061 gaacgggcag cgtctcatca tcaattaaaa cgaggtcagc atgtcagaac ttttaccgta
     3121 cctggaatcg gttgctcacg agatagacca catgatcgac cggtattttg tagacaaagt
     3181 cggggatctg aataaagcaa gtgcgcacct cctctctgca ggaggtaaaa ggctccgccc
     3241 tgccgtggtc atgctcgcag ccgatgcggt aaaacgggga agttctgaag atatcataca
     3301 ggctgcactt gcccttgagc tcacgcatac cttcacgctg atccatgatg atatcatgga
     3361 cgacgatacg ctcaggcgcg gcgtccagac ggtgcataca aaatgggata tgccgaccgg
     3421 tatccttgcc ggagatgtcc tgtacgcccg ggcttttgag tt
//
LOCUS       DASRLV010000200         3652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1040700,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000200 DASRLV010000000
VERSION     DASRLV010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040700"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VFC43_07620"
     CDS             complement(<1..359)
                     /locus_tag="VFC43_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZK31080.1"
                     /translation="MLHIEDLHVRVGDKEVLHDINLHIAEGETHVLLGPNGSGKTTLL
                     MTIMGFANYSITEGKILFKGENVTTMKAHERAKRGIGMLFQRPPTISGLKLGKMLKAI
                     SRNKENDILELAKSVHM"
     gene            482..1396
                     /locus_tag="VFC43_07625"
     CDS             482..1396
                     /locus_tag="VFC43_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HZK31081.1"
                     /translation="MRPEKVQDKVKKAGIRNSAQSTKITVSSEEPQEILPFVNRIICG
                     DAPKALAQIPDASIDLIITSPPYNFGQAYAQDPHDDTHEWNSYFEKLLVVWRECERVL
                     KPGGRMAVNIQPLFSDYVPTHHLISHQLAGLGLLWKAEFLWEKNNYNAKYTAWGSWKS
                     PSMPYIKYTWEFIEVFAKETHKKRGRREDIDITAEEFKEWVLGRWSFPPEIHMKDHGH
                     PAMFPEELPRRLMKLFSYKNDIVLDPFNGAGTTTLVAWKQHRRFIGIDTSVQYCDRAL
                     ERLSAVMENDFSSLSSYPAPDLMVIYTD"
     gene            complement(1419..2543)
                     /locus_tag="VFC43_07630"
     CDS             complement(1419..2543)
                     /locus_tag="VFC43_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31082.1"
                     /translation="MDSRVLKIIRIVLFVIIVVFSAVLINTFFFPRQAIIVPQIVPDV
                     ARLQSSPLLVSHQFPFENSAVILTIPVNASVYEGAKQSEKVTTVYGNISEAIWLAQSY
                     RSMVQDPTQDELYDTLIAETDAARLRQKLSDDEYVELLTVYVQSLRYQTREQNPVKFP
                     VETVVDRAGDCDDKSLLLAGLLSREGYPVVLLLFGPESHMAVGVGSDDYLYKETGYAF
                     VETTNYSFIGVPTDRLGGNLTLYSDPLIIPVSNGTKLYRSGSKTLYIHDMYESSDRNV
                     KELQPQIKSMGADLASQQEKIARIEKQMQDLRTSGDTGSYNAQVPFHNTLVSDYNARL
                     ETYRELFARYEKYVLIHNYILEHMYDRTGVFEYIKKNMPV"
     gene            complement(2580..3350)
                     /locus_tag="VFC43_07635"
     CDS             complement(2580..3350)
                     /locus_tag="VFC43_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HZK31083.1"
                     /translation="MKNIRGFLIDLDGVLYVGNREIQGAREAIEFLSKRKYMFRFVSN
                     TTRKCRKTIAERLSSMGFDIPVECIFTPPLAAVAFMKHSQKYRCFLLTTGDVYHDFEN
                     ECICDPVTPVDYVILGDAGDNVTYGSMNTAFRHLMNGADLIALEHDRYWMTPDGLSLS
                     AGPFVQALEFASGKKATIVGKPSKTFFELALRDMGLRANEAAMIGDDILTDIGGACSI
                     GMRGILVRTGKYRQETEDKAGVKPDCVIYSIGGLPDIL"
BASE COUNT          940 a          856 c          839 g         1017 t
ORIGIN      
        1 tccatatgaa ccgatttggc gagctccaga atatcatttt ctttgttccg ggagatggct
       61 ttcagcattt tacccagctt cagcccggag attgtcggtg gacgctggaa gagcatgcct
      121 ataccccgtt tagccctttc atgtgctttc attgttgtca cgttctctcc tttgaaaagg
      181 attttccctt ccgtgatact atagttggca aatcccatta tggtcatgag gagtgttgtt
      241 ttacctgacc cgttaggtcc aaggagaaca tgagtttccc cctccgcgat atggagattg
      301 atgtcgtgca atacttcttt atcgccgact ctcacatgca ggtcttcgat atgcagcata
      361 gattaatcgt agagaatatg tcgattacag cattaaacgc ttttcgaagt gaccaaaaaa
      421 atactggtgt ttcggatcct ttaaaacgtc atctggtaat gtactgacgt ggaagtattg
      481 tatgagacct gaaaaggtac aggacaaagt aaaaaaggca ggtatcagga atagtgcaca
      541 aagcaccaaa atcaccgttt cttccgaaga accgcaggaa attttaccct ttgtcaaccg
      601 cattatttgt ggagatgcac caaaagcact tgcgcaaata ccggacgcaa gtatcgattt
      661 gatcattact tctccaccct acaatttcgg gcaggcgtat gctcaggacc cccatgatga
      721 tacccacgaa tggaattcct attttgaaaa actcctggtc gtgtggcgtg agtgtgagcg
      781 tgtgctcaag cccggtgggc ggatggcggt gaatatccag cccctttttt cagattatgt
      841 ccccacacat cacctgatct ctcaccagct tgcaggcctt gggcttttat ggaaagccga
      901 gtttctctgg gagaaaaata actataatgc aaagtatacg gcatggggaa gctggaaatc
      961 tccgtcgatg ccatatatca agtacacatg ggagtttatc gaggtctttg ccaaggagac
     1021 tcacaagaag cgagggcgtc gggaggatat tgatatcact gccgaagaat tcaaggaatg
     1081 ggtgcttggc aggtggtctt ttcctccgga gatccatatg aaggatcacg gtcatccggc
     1141 catgttcccg gaggagttgc cacggcgcct gatgaagctg ttttcgtata aaaatgatat
     1201 tgtgctcgac ccgtttaacg gggcagggac aacgactctt gttgcatgga agcagcaccg
     1261 ccgttttatc ggtattgaca cttcagtcca gtactgcgac agggcattgg aaaggctctc
     1321 tgccgtgatg gagaatgatt tctcatcatt atcatcgtac ccggcaccgg atctcatggt
     1381 catatatacg gactgaacgt tcgttaaaat gtccggattc agaccggcat attctttttt
     1441 atatattcaa atactcccgt cctgtcgtac atatgttcaa ggatataatt atggataagg
     1501 acatattttt catagcgggc aaatagttcc cggtaagttt ccagccttgc gttataatcc
     1561 gaaacaaggg tattatggaa tggtacctgt gcattgtaac tgcctgtatc tccggaagtt
     1621 ctcaaatcct gcatttgttt ctcaatccgg gcaatctttt cctgttggga tgcgaggtca
     1681 gctcccatcg attttatctg aggttggagt tcctttacat tccgatcaga actttcatac
     1741 atgtcatgaa tatagagagt cttgcttccg ctcctgtaaa gttttgtgcc gttgctgaca
     1801 gggatgatga gaggatctga atacaaggtc aggtttcccc caagtctgtc ggttggcacc
     1861 ccgataaatg agtaattggt tgtttccaca aacgcatacc cggtttcttt gtaaagataa
     1921 tcatccgaac caactccgac tgccatatgt gattcagggc caaacaggag caggaccacc
     1981 ggatacccct cccgtgataa taatccggct aacagaaggc tcttatcatc acaatctccg
     2041 gcacggtcaa caacagtttc gacaggaaat tttaccgggt tctgttcccg ggtttgatat
     2101 cttaacgact ggacgtaaac ggtcaggagt tcgacatatt catcatcgga gagtttttga
     2161 cggagtcggg cagcatcagt ttctgcgata agggtgtcat agagttcatc ctgtgtcgga
     2221 tcctgtacca ttgaacgata actctgggcg agccagatcg cttcggatat gttcccatac
     2281 actgttgtaa ccttttctga ctgttttgca ccttcgtata ccgaggcatt gaccggaatt
     2341 gtcaggatga ctgcgctgtt ttcaaacgga aactgatggg aaaccaatag cggcgatgac
     2401 tgaaggcggg caacgtcagg aacaatctgg ggcacaataa tcgcctgccg ggggaaaaaa
     2461 aacgtattta tcagtactgc tgaaaagact acgatgataa cgaataatac tatacgaata
     2521 atttttagaa cccggctatc cattattcac cttcagtcat tctgttgccc gccgagatat
     2581 tacaaaatat ccggtaaccc tccaattgag tagataacac aatccggttt aacccctgct
     2641 ttgtcttctg tctcttgccg gtattttccg gtgcggacaa gaatgccccg catacctatg
     2701 ctgcaagccc cgccaatatc ggtgagaatg tcatcgccta tcattgctgc ctcattggca
     2761 cgaagtccca tatcgcggag ggcaagttca aaaaacgttt ttgagggctt tcccacaatt
     2821 gttgcttttt taccggatgc aaattcgagg gcctgaacga aaggacccgc tgaaagtgaa
     2881 agtccgtcag gtgtcatcca gtaccggtcg tgctcaaggg caatcaggtc tgcgccattc
     2941 atcagatgac ggaacgcggt attcatgctg ccataggtaa cattgtcccc ggcatctccc
     3001 aggattacat aatccacagg agttacagga tcacatatgc attcattctc aaaatcgtgg
     3061 tacacatcac ccgttgtgag gaggaagcaa cgatatttct gactgtgttt catgaaggct
     3121 actgcagcca gaggtggagt gaatatgcac tccacgggaa tatcaaatcc catggatgat
     3181 aaccgctcag caatcgtttt ccggcattta cgcgtggtgt tggaaacaaa cctgaacata
     3241 tattttcttt tgctgaggaa ttctatagcc tcccgcgcac cctgaatctc cctgtttcct
     3301 acatacagaa ccccatcaag gtcgatgaga aatcctctga tatttttcat tttttgtcct
     3361 ctcctgcagc attacagttc cggaagaatt ttttgtcccg tgtactcaca aattcttttt
     3421 tgttgcggga atatcctttt gccataaaag atgaccctgg gtttgtacct taaaaaatcc
     3481 gggcaagtct tgggaatccc ggtgaatatc actttcactc cccgcggccg gatgtttaaa
     3541 ccacaaacag catttttgta ttcggacgat gtcggcttca cacgtttctg cacggattgg
     3601 tgccctgtgg cagcaacgca tggcaaccct caaggaatat gaagttgtcc gt
//
LOCUS       DASRLV010000201         4798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_403102,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000201 DASRLV010000000
VERSION     DASRLV010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403102"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..1183
                     /locus_tag="VFC43_07640"
     CDS             56..1183
                     /locus_tag="VFC43_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HZK31084.1"
                     /translation="MDPLSDAVKKNGADAYVVYASSRDPDMRYLTHFTTSDPFVFFKK
                     PGQNGVIVVSTMETGRAAREASVAFMTRTQAGLPDILKKEKDPYKATAAMIAGLVGKN
                     VLIPPNFPVALANALGEHCTLSIDKGTVMEMRSRKSRPEILAMKKVQKVTQQAMGLAV
                     SLIRNGSVKKGNLHIDGKPLTAERVKYTMHNLLLKHGCSAVDTIVSCGEDTAIPHIAG
                     TGILRSDEPIVIDLFPVDERSGYYADMTRTVVKGEPSPEILAMYNALREAKQLGISKI
                     KAGVPGADVHQMVVDFFKDRGYESGTRGFVHNLGHGVGLQVHELPTVGPAGNILTTGN
                     VITIEPGLYYPGIGGVRLEDIGTVTAHGFDNFTLFPEDLVV"
     gene            1180..2055
                     /gene="map"
                     /locus_tag="VFC43_07645"
     CDS             1180..2055
                     /gene="map"
                     /locus_tag="VFC43_07645"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II methionyl aminopeptidase"
                     /protein_id="HZK31085.1"
                     /translation="MKDEIFEKYRNAGEIAAKILRRGAKEIREGVSYLEVVESVEVQV
                     GEEGAALAFPLNLSLNEDAAHDTASPGDTRVFSRGDVAKLDLGVQVDGYIADTAITID
                     LGNNSLLLEASEKALDAAIKAVRPGTTAGELGAAVQKEIEYRGYRPIANLTGHGLDRY
                     ILHRSPTIPNIGVNGGVILEEGMVFAIEPFATTGSGHVGEKTRKEIYSQISQKPVRTP
                     VARNILNSVKERRGLPFARRWLSEIKPDIALSALVRSQILHVYPVLSDILGSLVSQHE
                     HTVIVTDEGCIVTTR"
     gene            2101..3375
                     /locus_tag="VFC43_07650"
     CDS             2101..3375
                     /locus_tag="VFC43_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK31086.1"
                     /translation="MSGDSEVLRIMEIVLTAEIFNQNPQLDINDLTPACREIFSFTTS
                     ADIKRPVFISDGLIKRSLAIADAHQKLSANPFIAYEEFGQRIRVTALEPAAQWFLRQG
                     GKSIIEKNPALAFFYENIDSTGIAYATVRAKNPPFEDTKAYLDARISKLIGEDEKLRG
                     ALDLVILLTPEEVEQQMEDLVCTPEQTAIIAKIQLAVNHREFLHRHRIHEVGKLLFVG
                     PPGTGKTSLALAMSRAMHMPVLEVRLSMVTSQYLGETSKNIDRIFELAKKLAPSILFI
                     DEFDFVAKSRIADDHGAMKRAVNSLLKNIDKINLVKNNVLLIGATNHPQLLDEAAWRR
                     FDEVVEFSLPNEEMRKNILMKVTSSFQCSLDYQQLASQTEGFSGSDLRMMVKEAILSS
                     LSANRKTIEREDIEKGIHMVKNRDAIRHLTWI"
     gene            3377..4099
                     /locus_tag="VFC43_07655"
     CDS             3377..4099
                     /locus_tag="VFC43_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZK31087.1"
                     /translation="MKITLLGTGDAIGTPKVGCTCPQCSHAKKTGVMRLRTSLLVENE
                     GRHILIDSTPDLRHQLLLYDSPHIDAVIWTHGHYDHYIGFGEFYRVQKIPPVFAAPLV
                     LNYCAETFRFLTFDKGEVQPYEPFEIFGITITLFIVKHPPAFTCGILFETADSRVGFT
                     SDTNLDIPQKSLDLLSNLDLLLLDAIVPSNITIHKHMNYLDACTLARDLSPKEYRCVH
                     LSHLIPWDLPHLGKDGDVFEFR"
     gene            4177..4446
                     /locus_tag="VFC43_07660"
     CDS             4177..4446
                     /locus_tag="VFC43_07660"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit L"
                     /protein_id="HZK31088.1"
                     /translation="MIVKVLDLEKDKVRLIIKGEGHTFMNALVEEILNDPDVDVATYM
                     IEFQFSDPELLVTTKGKKNPLPIIKKACKRISGHCNELIKDMKKN"
     gene            complement(4661..>4798)
                     /locus_tag="VFC43_07665"
     CDS             complement(4661..>4798)
                     /locus_tag="VFC43_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328536.1"
                     /note="eIF-2B; functions in the early steps of protein
                     synthesis by forming a ternary complex with GTP and
                     initiator tRNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit beta"
                     /protein_id="HZK31089.1"
                     /translation="SKRGDGVVKMGRYIMYVANAKPGQKVKIKISRISGSIVFTERAE
                     E"
BASE COUNT         1335 a         1120 c         1110 g         1233 t
ORIGIN      
        1 gtacatcaac tccgaaataa aagtacttga cgtttaacta catatagaat gagagatgga
       61 tccactttca gatgcggtta aaaagaacgg tgctgacgcc tatgttgtct atgcgtcatc
      121 ccgagatccc gatatgagat acctgacaca ctttacaaca agcgatcctt ttgttttttt
      181 taaaaaaccc ggacagaacg gagtgatcgt ggtgtccacc atggaaacag ggcgcgcagc
      241 acgtgaagct tccgttgcat tcatgacaag gactcaagcc gggctcccgg atatcttaaa
      301 aaaagaaaag gacccctata aagccactgc ggcaatgatt gcggggcttg tcgggaaaaa
      361 cgttcttatc ccgcctaatt tcccggtcgc gctggcaaat gcactgggtg agcactgtac
      421 tctttcaatc gacaaaggaa ctgtcatgga aatgaggtca aggaagagca gacctgaaat
      481 ccttgctatg aaaaaagtgc agaaggtaac acaacaagca atgggattag cagtttcgtt
      541 aatccgcaat ggttctgtta aaaaaggaaa cctccatatt gatggaaaac ccctgactgc
      601 cgaacgggta aagtatacca tgcataacct gcttctgaaa cacggttgta gtgcagtgga
      661 cacgatcgta tcatgcggag aagataccgc gataccacac atcgccggaa cagggatcct
      721 cagatcagat gaacctattg tcattgacct ttttccggta gatgagagat cgggatacta
      781 tgccgatatg acccggactg tggttaaggg tgagccttcg ccagaaatcc ttgcaatgta
      841 caatgcgctc cgggaggcaa aacaactcgg gatttccaag attaaagccg gagtgcctgg
      901 agcggatgta catcagatgg ttgttgactt ttttaaagat cgggggtatg aaagtggtac
      961 acggggcttt gtgcataatc tcggtcacgg ggtcggtctt caggtccacg agctgccaac
     1021 tgttgggcca gctggaaata ttcttacaac cggtaatgta atcaccatcg aacctggtct
     1081 ctactatccc ggcatcggcg gcgtgcgcct tgaggatatc ggcacggtca ccgcacacgg
     1141 atttgataat ttcacactat ttccagagga tctggtcgta tgaaggatga gatttttgaa
     1201 aaatatcgta atgccggaga gatcgcagca aaaattcttc gaaggggtgc aaaagaaatc
     1261 cgcgaaggtg tatcctatct cgaagttgtc gaatctgttg aagttcaggt aggtgaagag
     1321 ggggctgcgc tcgcatttcc gctcaacctg tccttaaatg aagatgccgc acacgacact
     1381 gcttcaccgg gtgatactcg ggtattctcc cggggcgatg tagcaaaact ggaccttgga
     1441 gtccaggtcg atgggtatat cgccgacacg gcaattacga ttgacctggg gaacaactca
     1501 ctcctcctcg aggcttcaga aaaggcactt gatgcggcga taaaagcggt ccggccggga
     1561 acgaccgccg gggaacttgg ggctgccgtc cagaaagaga ttgaatacag gggataccgc
     1621 ccgatagcaa accttaccgg tcatggactt gaccggtata ttctccatcg ctctccgaca
     1681 attccaaata tcggtgtcaa tggaggggtt atactagaag aggggatggt ttttgccata
     1741 gaaccattcg caaccaccgg aagcggacat gtgggagaaa aaacccgtaa ggaaatttac
     1801 tcccagattt cccagaagcc cgtcagaact cccgttgcac gtaatatcct taatagtgtt
     1861 aaggaacgtc gtggtctgcc ttttgccaga agatggctga gtgagataaa accagatatt
     1921 gcactttctg cactggtgag atcgcagatc cttcacgttt atccggtcct gtccgatatt
     1981 ttaggatctc ttgtttccca gcacgaacat acggttattg tgaccgacga aggttgtatt
     2041 gttacgactc gctaaagata ggcttattca ttccggagat gaatagataa ggttacagct
     2101 atgtctggtg attctgaagt ccttcgaata atggaaatcg ttctgaccgc tgaaatattc
     2161 aaccagaacc cccagctcga tattaatgac ctgacgccgg cctgccgcga aattttttca
     2221 ttcaccacat ctgcggatat caaaaggcct gtcttcataa gcgatggcct catcaaacgc
     2281 tctctggcta ttgcggatgc gcaccagaaa ttatcagcaa atccgtttat tgcttatgag
     2341 gaattcggac aacgtatccg tgttaccgct cttgaaccgg cagcgcaatg gtttctccgg
     2401 caaggcggga aatccattat tgaaaagaat cctgcactcg cctttttcta tgagaatatc
     2461 gattccacgg gtatcgccta tgcgacagtc cgtgcaaaaa atccgccatt tgaagataca
     2521 aaagcgtatc ttgatgcacg catatccaaa ctgataggtg aggacgaaaa actccgtggt
     2581 gcacttgacc tcgtcatcct cctcacacca gaagaggtgg agcaacagat ggaggacctt
     2641 gtttgcactc cggaacagac cgctatcatc gcaaaaatac aacttgctgt caaccaccgg
     2701 gaattcctcc accggcacag gattcatgaa gtagggaaac tcctctttgt tggacctcca
     2761 ggtaccggaa agacgtcgct tgcacttgca atgtcccgtg ccatgcatat gccggtcctt
     2821 gaagtccggc tttcaatggt cacctcccag tatcttggag agacttcaaa aaatattgac
     2881 cgcattttcg aacttgccaa gaaacttgcc ccatctattc tttttattga tgaattcgat
     2941 tttgttgcca agagtcgtat agcggatgac catggggcaa tgaaacgtgc agttaactcc
     3001 cttttaaaga atatcgataa gatcaacctg gtaaaaaata atgtcctcct gattggtgca
     3061 acaaatcatc cgcaacttct cgatgaagca gcgtggcgca ggttcgatga agttgtggaa
     3121 ttctcactcc cgaatgaaga gatgcggaag aatatcttaa tgaaggtcac ttcctcgttc
     3181 cagtgcagcc ttgattacca gcagctggct tcccagactg agggcttttc cggttccgat
     3241 ctccgcatga tggtcaaaga ggcgattctt tcttcactca gtgccaaccg gaaaacgatc
     3301 gagcgcgaag acatcgaaaa gggtattcat atggtaaaaa accgggatgc gatacggcac
     3361 ctgacatgga tataatatga aaatcactct tcttggaacc ggggatgcaa ttgggacacc
     3421 gaaagttgga tgcacatgtc cccagtgcag ccatgcgaag aagacaggag tgatgcgcct
     3481 gaggacatcg cttcttgtag agaatgaggg aagacacatc cttatcgatt caacaccgga
     3541 tctacgccat cagcttctcc tgtatgattc cccgcacatt gatgctgtga tttggactca
     3601 tggacattat gatcattata tcggatttgg tgagttctac cgtgttcaga aaattccccc
     3661 ggtatttgct gcacccctgg tgctcaacta ctgtgctgaa acattcaggt ttttgacttt
     3721 tgataaagga gaagtccagc cttatgagcc atttgaaata tttggcatta caattacgct
     3781 gttcatcgta aaacatcccc cggcatttac ctgtggcata ctctttgaaa ctgcggactc
     3841 gcgggtgggt tttacctcag ataccaacct tgatatccca caaaaaagcc tcgatcttct
     3901 ctcaaatctt gaccttctgc tccttgatgc gattgtgcca tcaaatataa caattcacaa
     3961 gcacatgaat tatctcgacg cctgcacact tgcccgtgat ctctccccaa aggaatatcg
     4021 ttgcgtacac ctgagccacc ttatcccctg ggaccttcct catctcggta aagatggaga
     4081 cgttttcgag ttccgttaga ttttttttta tcattgatta ttggcttccg gcagtaccca
     4141 tactttaata gttttcaggt gcccaaagat aggtgcatga ttgtaaaagt tctcgatctc
     4201 gaaaaagata aagtgcgact catcatcaaa ggtgaagggc atacgttcat gaacgctctt
     4261 gtggaagaga tattaaatga cccggatgtc gatgttgcaa cgtatatgat tgagtttcag
     4321 ttttcagatc cggaacttct tgttactacc aaagggaaga aaaatcccct tccgataata
     4381 aaaaaggcat gcaagcggat atcgggtcat tgcaatgagc tcatcaaaga catgaagaaa
     4441 aactgattcc agcatcattc taacaaaata acatatcttt ttaaaaacca tccttcaaaa
     4501 tccggtccaa cctctcattg gagaacattc ataagagtta taaaaagggt ttgggataca
     4561 atttgtgata taccctgctg aaaaatgctc ctctgtgtga aattcccagt aacagggatg
     4621 aggtgcgcta aaaattgagg gacttgggtt ttataataaa ctattcctca gctcgttccg
     4681 taaatacaat cgatccggag atacgggaaa tcttgatttt taccttctgt ccgggctttg
     4741 catttgccac gtacatgata taacggccca tcttgacaac cccgtctccc cgttttga
//
LOCUS       DASRLV010000202        12731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_891383,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000202 DASRLV010000000
VERSION     DASRLV010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 12731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12731
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891383"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VFC43_07670"
     CDS             complement(<1..376)
                     /locus_tag="VFC43_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31090.1"
                     /translation="MKAPHILAALLIASVLANIGLIAFVIPSEQGIISQLVDKTNTLG
                     TEKMQLQKQLDQQNITLQSYTSQLSFYRMNPTGSFTGSQSSDRFITGYATLQAPAVSQ
                     HIVQETINGFVTQRLVQNGSMMN"
     gene            complement(802..948)
                     /locus_tag="VFC43_07675"
     CDS             complement(802..948)
                     /locus_tag="VFC43_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31091.1"
                     /translation="MKTLISGKNNAPACTTSPPLGDKANTAITRVWQQVLHRRRNENA
                     RRRG"
     gene            1053..1439
                     /locus_tag="VFC43_07680"
     CDS             1053..1439
                     /locus_tag="VFC43_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZK31092.1"
                     /translation="MGKNVLVVEDDAIIQQVLEWRLNTLGYTVCGKAATSDEAITCVR
                     EKKPDAVLMDIDLGGLVDGIDTAVAIKKMHKVPVIFLTASSTQADIDRAKMVPADGFI
                     LKPFNDTDIRVALTLAIPDTGPKLPE"
     gene            complement(1515..2024)
                     /locus_tag="VFC43_07685"
     CDS             complement(1515..2024)
                     /locus_tag="VFC43_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HZK31093.1"
                     /translation="MIFIAPGGIRLHYRKMAGRLILSKLHHQNMKKYWIIVASEDHVM
                     RGVSLGIAQAGHGKRSGLARMHKGDMMVYYSPKARLEGSEALHAFTAIGEIADDAIFQ
                     VEETRDFKPFRRKVHYVKTGEVRIEPLVQDLTFIRNKKSWGYAFRYGLLQIQEDDFDR
                     IREKFPADL"
     gene            2014..2349
                     /locus_tag="VFC43_07690"
     CDS             2014..2349
                     /locus_tag="VFC43_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31094.1"
                     /translation="MKIKPGPFPYPFIPHFSRLPECFGELRSLKMQTSFPENNSYYQR
                     PYDPLLSKILVISRLKEWQWKLITPKSVFAGFPADCALITTLKVQAGAGDVKPNPGWA
                     PVARLSRVP"
     gene            2313..2756
                     /locus_tag="VFC43_07695"
     CDS             2313..2756
                     /locus_tag="VFC43_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31095.1"
                     /translation="MGAGCPFITCALKKKGIEFCWNCEENETCVKWARHRESSKKVDS
                     FTCYQKLKDDIGFIQKHGVSEFEKTQKNKEHLLKEMLQGFNEGRSKSYYCIAATVMDI
                     GELEEALDKARKNSDGLAIQEKSGVLHSLLDEIARQRNYYLKLRK"
     gene            complement(2907..4019)
                     /gene="thiI"
                     /locus_tag="VFC43_07700"
     CDS             complement(2907..4019)
                     /gene="thiI"
                     /locus_tag="VFC43_07700"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HZK31096.1"
                     /translation="MMKKQWLIRYSEIFLKSDPVRRRWENVLIKNIKEMMPDVYMRSE
                     RGRIWLDGDVKPDRLKNIFGIVSFSETEHIQLEELEGFLPDYCRRHDIEKAKTFAIKM
                     KRVGTHTFSSNDKAIEYGNLIRNKFPHLKVNLAHPDKEIHIEIRANEVYLYDTVIQAV
                     GGLPLGVEGTLVALVSGGIDSPVAAWMMMKRGCRIIPLFVALDTFLDETTIARAKSVV
                     EKLAKYQPGIELIVVSDSYLATAKEELVRRHLEKYTCIFCKRRMYRVATAFADKIGAK
                     GIVTGESLGQVASQTLDNLMVLNDAAEIPVYRPLIGFDKEETIRIAREIGTFEQSISN
                     ASGCTAVPKGASTNAKMDAVHKIERMLGATSIPLPI"
     gene            complement(4038..4328)
                     /locus_tag="VFC43_07705"
     CDS             complement(4038..4328)
                     /locus_tag="VFC43_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1894 domain-containing protein"
                     /protein_id="HZK31097.1"
                     /translation="MGCIENLKYEMILPLGASFKECREYAEKNFREVWYVDPGYKLFD
                     EYIIGLPPIALGIEEGKKIIFPYTKPCHGTYLLAVEDAEEVERIRKTARKKK"
     gene            complement(4307..4792)
                     /locus_tag="VFC43_07710"
     CDS             complement(4307..4792)
                     /locus_tag="VFC43_07710"
                     /inference="COORDINATES: protein motif:HMM:NF020570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1890 domain-containing protein"
                     /protein_id="HZK31098.1"
                     /translation="MMTDIPATPKKALIVLGCPEAPAQQALAFHVVHNLRKQSTDVLI
                     AGNPSVLNLLKVSDPEKHYITDTMVLEKCIAEVVEKKRDCDLCVVFAHNDVAIAYAAT
                     MRHLLPERRLVVIIFGKDPGPLADEINFPCEKIVDKAVHNPMQLRAKINEVFGWVVSR
                     T"
     gene            complement(4789..5343)
                     /locus_tag="VFC43_07715"
     CDS             complement(4789..5343)
                     /locus_tag="VFC43_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonyl-tRNA synthetase editing
                     domain-containing protein"
                     /protein_id="HZK31099.1"
                     /translation="MRILSIHASSMWYHATQKTKLAEPITVHEDRMDECVVLFCCIEK
                     LDEKNPDKVITSAKQNILQRLDKLKVRKVLIYPYAHLTSTLGSPEVAFSILKGLEESL
                     HRERVEVKRAPFGWYKEFEIRGKGHPLADLSMTICPYEGTECDFTCPYCHHPFREADA
                     EHVCQQSAECTGACPGKQAIIGER"
     gene            complement(5344..6831)
                     /locus_tag="VFC43_07720"
     CDS             complement(5344..6831)
                     /locus_tag="VFC43_07720"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HZK31100.1"
                     /translation="MIKGSVGTVTTHFYHHPSPLILESGDVLPSLTIAYEIYGKLNQE
                     RNNAILVCHALSGDSHIAGFHAGDDKPGWWDAVVGPGKAFDTDRYCVICQNVIGGCKG
                     STGPSSENPATGKPYGATFPVITIRDMVHAQKLLIDHLGIPQLYAVAGGSMGGMQALQ
                     WTVSYPDTVRKAIIIAATGYSTPQQIAFNEVGRKAIISDPEWNGGNYYDTKSYDAHGP
                     VRGLALARMVGHITYLSDESMHAKFGRSLQAKDRIGFNFSTDFAVESYLHHQGDTFTR
                     RFDANSYLYITKAIDYFDLTKDGSLAKGLAGVRAGFLIISVSSDWLYPPYQSQEIVSA
                     LTANECEVRYSEIRSNYGHDAFLLESGQINYLITQFLSRTVVGDVMIRDVPTIEEGAT
                     TAVTARRLITLEVNHLPVLSVSGQLAGIVTSWDIAKAVAKNFLWLDEIMSKNVITTAP
                     DEPIESAAQKMEEHSISALPVVDEDNHLIGLITSDAISTLIGRGN"
     gene            complement(6917..8212)
                     /locus_tag="VFC43_07725"
     CDS             complement(6917..8212)
                     /locus_tag="VFC43_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HZK31101.1"
                     /translation="MADKEFRLGTTALHAGQVPDPTTGSRTVPIYQTSSYVFKSAEHA
                     ANLFGLRELGNIYTRLMNPTTDVFEKRMAAIEGGTGALAVASGQAAITYSVLNITRPG
                     DEIVAADNLYGGTYELFNYTFPKFGRKVVFVDSTNPAAFKNAITEKTRAIYAETIGNP
                     KLDIPDFVTLAKIAHDAGIPLVVDNTTGVGLVRPIRHGADIVVHSATKYIGGHGNSIG
                     GVIVDSGKFAWNNGKFPEFTDPDPSYHGLKYWDAFSNFPGAGNIAFIFRIRLSLLRDT
                     GAALSPFNAWLFLLGLETLHLRVPRHSRNAMAVAEFLTTHPKVAWVNYTGLPGHQSHA
                     LAKKYLVGGFGPLVGFGVKGGEKASLKVIDSLRLFSNLANIGDSKSLVIHPASTTHQQ
                     LTVEEQKKTGVTPDAIRLSVGTEDIEDIIADLEQALAVV"
     gene            complement(8223..9587)
                     /gene="pscS"
                     /locus_tag="VFC43_07730"
     CDS             complement(8223..9587)
                     /gene="pscS"
                     /locus_tag="VFC43_07730"
                     /EC_number="2.5.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-phospho-L-seryl-tRNA:Cys-tRNA synthase"
                     /protein_id="HZK31102.1"
                     /translation="MSIRVQKTFEALFALEEIRGLFRESLPTGFDAGEEAAFKERIAD
                     LKAIVADLEAGTGSPKVTKIAGSIDIRSREEQYINIHPIQAAGRLTTEARKAIISYGD
                     GYSTCDACRKPFRLDKISKPGIADFHAGLAQWLNMDQARVVPGARRGFQAVAGTLVGK
                     DDSVIVSALAHYTEFLAVENAGGVVKEVPLNAKNIVTAEAMAQKIEEVKAETGKLPVL
                     VIIDHFDYQFANEHEVAGIGKVAHQYDIPFLYNGAYTVGVMPVDGKKIGADFVVGSGH
                     KSMASVAPSGVLAMTDEWIPKALRTTTMVGDLTKRKFGIKEVEMLGCTLMGGTLLSMM
                     ASFPTVKERVLHWDEQVKRSNYFIDRLLKISGSTVLSEYPRKHTLTKIDTTGSFDTIA
                     KTHKRKGFYFSDELSSRGIVGEFAGATRTWKLNTYGLNDRQIHYLADVFTEIAEKYEI
                     PVTK"
     gene            complement(9598..9669)
                     /locus_tag="VFC43_07735"
     tRNA            complement(9598..9669)
                     /locus_tag="VFC43_07735"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9635..9637),aa:Cys,seq:gca)
     gene            9906..10496
                     /locus_tag="VFC43_07740"
     CDS             9906..10496
                     /locus_tag="VFC43_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31103.1"
                     /translation="MHSTPWSTGITICGAGALLLVLILVSGCTGTVQPPLVTGPPGIS
                     PNDTDRSWMDVPLMDLQGKGNFSPGSFAGKSVVLLVVSDACPACVSQLSREIGEIEGL
                     ARVQDGSIVFVALDLDPTGDPGFITKYHDQFNFTGYTARSSEEMTLKLFRELGPFAID
                     TEAIPVILICPDRHEVLLPPGFKPTDKLDTRYTQEC"
     gene            10500..11180
                     /locus_tag="VFC43_07745"
     CDS             10500..11180
                     /locus_tag="VFC43_07745"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HZK31104.1"
                     /translation="METFTLFWLSFLAGIHASAGAPCALVMYPGYLAFLAGKSGDERY
                     RHEPFTLGILVAIGVIAAMLTGGILFSFAVAEGGGAVRNLIAPAAFILLFFFSLFIIF
                     DINFGKIPVANPIPMVKNPLAAAFFLGLAFGIIILPCNAAAVVVLLALAATATGLFEG
                     LGAFLSFGLGITLPLVILAGLSRAAGQQVMEYLTRHRRAIRLTGGIIMLIISVWYLAL
                     LFLPQMSR"
     gene            complement(11262..11837)
                     /locus_tag="VFC43_07750"
     CDS             complement(11262..11837)
                     /locus_tag="VFC43_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HZK31105.1"
                     /translation="MKVIAFNGSARKDGNTAILVNTVFKELKKAKIETELVQLAGKKI
                     SGCIACGKCFENKDKRCVVVSDILNECIEKMLEADGIILASPTYFADISANMKALIER
                     SGFVARANNDMFRRKVGASVVAVRRAGAIHAFDTMNHFFFISQMIVPGSSYWNVGIGL
                     GPGEVKKDEEGLLTMKTLGTNMAWLMKKLEK"
     gene            11989..12219
                     /locus_tag="VFC43_07755"
     CDS             11989..12219
                     /locus_tag="VFC43_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HZK31106.1"
                     /translation="MDYRRTPMAEDEFQFMAVDKVTETLHTSLQGLTPEVVESKRARY
                     GPNEVVREKKRAAFRQFAGQFKNPLIIIILTG"
     gene            complement(12256..>12731)
                     /locus_tag="VFC43_07760"
     CDS             complement(12256..>12731)
                     /locus_tag="VFC43_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZK31107.1"
                     /translation="SVLPSSEARIILESLNKENAKKIQSIIGKQEEKVINYTTRKFLK
                     FSPQTTVEYVQNDYPRHARDKDVIMYIYVVDDLDVLVGVIDLKELLQADDRALLKDIM
                     IENVISLNTDSTLKEASQSFARYDFRALPVTDEKNRLVGVIPYRDVMNLTHHFLE"
BASE COUNT         2964 a         3234 c         3175 g         3358 t
ORIGIN      
        1 tgttcatcat cgaaccgttc tggaccagtc gctgggtcac aaatccgttg atggtttcct
       61 gcacgatatg ctgggacact gccggtgcct ggagtgttgc atacccggtg atgaatctgt
      121 cagaagactg gctgcccgta aaagaaccgg tcgggttcat ccggtagaaa ctgagctggg
      181 atgtatagct ctgcagggtg atattctgct ggtccagttg tttctggagc tgcatcttct
      241 cggtgccaag agtgttggtc ttatcgacaa gctgggaaat gatgccctgc tctgaaggaa
      301 tgacaaacgc gatcagtccg atatttgcaa gaactgaagc aatgagcagg gcggcaagga
      361 tgtggggtgc cttcatgtct catactccgt aactgtcatg atcttcttat tcttggagaa
      421 acacagttaa agtgttttta gtgcggtggc agtctaaaag aactgggaat tggatttagt
      481 gggggggagg gtgtgaagaa ttatttgttg tgttcatctt ttttattatc aacaaagccc
      541 gtaaatccaa ttcttacaac atgaagggga tgcggggtta tatattgctc aatcgctgct
      601 ggttcagtca tgaaaccaac cggtttaaac gtggatgctc aaagattgat cttcgttgct
      661 ggtcctgggt ttgaatttgt aaattacata aataaaagtc acctgttcat tccggtcagg
      721 gaaatgtccg gaatttcaga attttgagct tttattgctc tggagaaata cgtctgaaaa
      781 ttctgaggca cttttgggct tttacccccg ccgccgggca ttctcattac gacgacgatg
      841 tagtacctgc tgccagaccc gggttatcgc cgtgttcgcc ttatccccca gcgggggact
      901 ggtggtgcaa gcgggggcat tatttttgcc agaaatcagg gtcttcaaag agcagatttg
      961 ataaaaaatc aaagaaataa caactgttta acaaactatt tatataaata atctggaatt
     1021 taaaacaaag gataaagtgg gagttatttt caatggggaa gaatgtcctt gtcgtggaag
     1081 atgatgcgat aatccagcag gtacttgaat ggcggctgaa taccctggga tataccgtat
     1141 gtggcaaagc cgcgacatca gatgaggcaa taacctgtgt cagggaaaag aaacccgacg
     1201 ccgttctcat ggatatcgac ctgggtggac ttgtggacgg gatcgatacc gcggttgcaa
     1261 ttaaaaaaat gcataaggtg ccggtgattt tcctcacggc ctcgtcaact caggcagata
     1321 ttgaccgtgc aaaaatggtg cccgccgatg gatttatttt aaaacctttc aacgacacag
     1381 atatacgggt tgccctgaca cttgctattc cggataccgg cccaaaactc ccggaataaa
     1441 cggatctcat ctgtttacaa aaacgttttt cctgacggga tctcccttac gaccctgtta
     1501 cttttttagc ccccttataa atccgcggga aatttctccc tgatacgatc aaaatcatcc
     1561 tcctgtatct gcagcagacc gtacctgaac gcgtatcccc aggacttttt attcctgatg
     1621 aaggttaaat cctgtaccag gggttcaatc ctgacctcac cggttttcac ataatggacc
     1681 tttctccgga acggtttgaa atcccgggtt tcttccacct ggaatatcgc atcatccgca
     1741 atctcgccaa tcgcagtaaa tgcatgaagg gcttcactgc cttctaacct tgcctttggg
     1801 gaataatata ccatcatgtc acctttgtgc atccgggcaa gaccggaccg tttcccatgc
     1861 ccggcctggg ctatccccag agaaacaccc cgcatgacat gatcttctga tgcaacgatg
     1921 atccagtatt tcttcatatt ctggtgatgt aactttgaga gtattaaacg tccggccatt
     1981 ttccggtaat ggagccgtat acctccgggt gcgatgaaaa tcaaacccgg gccatttcct
     2041 taccccttta ttcctcactt ttcgcgattg ccggaatgtt ttggtgagct ccggtctttg
     2101 aaaatgcaga cttcatttcc agagaataat tcttactatc agcggccata tgatccatta
     2161 ttgtcaaaaa tactggtgat atcacggtta aaggaatggc aatggaaatt aattaccccg
     2221 aaatcggtat ttgcgggctt tcctgctgac tgtgccctca ttaccacact gaaggtgcaa
     2281 gcaggtgcgg gggatgtaaa accaaatcca ggatgggcgc cggttgcccg tttatcacgt
     2341 gtgccctgaa gaaaaaagga attgaattct gctggaattg cgaagagaat gaaacgtgtg
     2401 ttaaatgggc aagacataga gaatcttcaa aaaaagtcga ctcattcacg tgttatcaaa
     2461 aacttaaaga tgatattgga tttatccaga agcacggggt aagcgagttt gaaaagacgc
     2521 aaaaaaataa agaacacctg ctaaaagaga tgctgcaggg atttaatgaa ggacgttcaa
     2581 aaagctacta ttgcatcgca gctaccgtga tggatatcgg agaattagaa gaagcattgg
     2641 ataaagctcg taagaattct gatggattag cgatacaaga gaaatccgga gttcttcatt
     2701 cactcttgga tgaaattgcc cggcagagga actactatct gaaattgaga aaatgaaaag
     2761 acgttttttt caccttaagc acggtcaaca gagtccaccc cgaccggata tctcccggtc
     2821 ctgtttacct tttagatcct gacgatactg agctctgtta tcgtggaaac cgatgacacg
     2881 accggggacc tcagaatagt accccgtcag atcgggagtg gaatcgaggt cgcgcccagc
     2941 atcctctcga ttttatggac tgcatccatt ttcgcattcg ttgatgcccc cttgggaacc
     3001 gctgtgcatc cggatgcatt tgagatggac tgctcaaatg tcccgatctc gcgggcgatc
     3061 cggattgttt cctccttgtc aaacccgatc aacgggcggt agactggaat ctctgctgca
     3121 tcattcagca ccatgagatt gtccagtgtc tggctcgcaa cttgtccgag ggattccccg
     3181 gtcacgatac cttttgctcc gatcttgtct gcgaatgccg tagcaacccg gtacattcgt
     3241 cgtttgcaga agatgcaggt gtacttttca agatgcctgc ggaccagttc ctcttttgcc
     3301 gtggcaagat aggagtctga aaccacgatc agttcgatgc caggctgata ttttgcgagt
     3361 ttttcaacga cactctttgc acgtgcgatc gtggtctcat caaggaatgt atcgagcgcg
     3421 acgaagagcg ggatgatacg gcagcccctt ttcatcatca tccatgcggc aaccggcgag
     3481 tcaatacccc cggaaacgag ggcaacaagc gttccttcaa caccgagcgg cagacccccg
     3541 accgcttgta tcaccgtgtc gtagagatac acctcattcg cccgtatctc gatatggatc
     3601 tccttatcgg gatgggcaag gttcactttg aggtgaggga atttgttcct gatcaggttg
     3661 ccgtactcaa ttgccttgtc gttagaggaa aaagtatgag tgccgacccg cttcatcttg
     3721 atcgcaaatg tctttgcctt ttcgatgtca tggcggcgac agtaatctgg caggaatcct
     3781 tcaagttctt cgagctggat gtgctctgtt tctgaaaatg acacaatccc aaatatgttt
     3841 ttcagtctgt ctggttttac atcgccgtcc agccagatcc tgccccgttc gctgcgcatg
     3901 taaacatccg gcatcatttc tttgatattt ttgatgagta cgttctccca tcgccgccgg
     3961 accggatcgg attttaagaa tatttcagaa taccggataa gccattgttt tttcatcatg
     4021 ccggcccata aaaagtatta ttttttcttt cgtgcggttt tgcgaatacg ttcaacttcc
     4081 tcagcatctt cgacagccag caggtaggtg ccgtgacagg gttttgtata cggaaagatg
     4141 atcttcttac cctcctcaat gccgagggca atcggcggga gaccgataat atactcgtca
     4201 aagagcttgt aacccggatc aacataccag acctctctga aattcttttc tgcatattcc
     4261 cggcactcct tgaacgaggc cccaagcggg aggatcatct cgtacttcag gttctcgata
     4321 caacccatcc gaacacctcg tttatcttcg ctctcagctg cattgggtta tgcaccgctt
     4381 tgtccacgat tttctcgcac gggaaattta tctcgtcggc aagcggcccg ggatcctttc
     4441 caaagataat caccaccagt ctccgttccg gcaacaggtg gcgcattgtt gccgcatagg
     4501 caatcgctac gtcgttgtgc gcaaagacaa cgcaaaggtc acagtcccgt tttttctcga
     4561 ctacttcggc gatgcacttt tccagaacca tcgtatccgt gatgtagtgc ttctcaggat
     4621 ctgaaacttt caggaggttg aggacggagg gattccccgc gataagaacg tcggtacttt
     4681 gctttctcag gttatgcacc acatggaacg cgagcgcctg ttgtgccgga gcttccgggc
     4741 acccaaggac gataagtgct ttttttggtg ttgctggtat atctgtcatc atctttctcc
     4801 gatgattgcc tgtttgccag gacaggctcc tgtgcattca gcagactgct ggcagacatg
     4861 ttccgcatca gcttccctga acgggtgatg gcagtacggg cacgtgaagt cgcattcagt
     4921 accttcgtac gggcagatgg tcatggagag atcggcgagc gggtgcccct tgccccggat
     4981 ctcgaactcc ttataccagc cgaatggtgc ccgtttcact tcaacccgtt ctctatgaag
     5041 cgactcttcg agccctttca gaatggagaa tgcgacctca ggtgacccga gggtgcttgt
     5101 gaggtgagca tacgggtaaa tcaggacttt tctgacctta agtttatcaa gtcgctgcag
     5161 gatattctgt tttgcactgg taattacctt gtcaggattt ttctcgtcca gtttctcgat
     5221 acaacagaag aggacaacgc attcatccat acggtcttcg tgcaccgtaa tgggttctgc
     5281 cagttttgtc ttctgcgtcg catgatacca catggaggat gcatggatgg agaggattct
     5341 catctagttc cccctcccga taagtgtact gatcgcatca ctggtaataa ggccgatgag
     5401 atggttgtct tcatcaacaa ccggcagggc agagatggag tgctcctcca tcttctgcgc
     5461 agcagactcg atcggttcat ccggggctgt tgttatgaca ttttttgaca taatctcatc
     5521 aagccagagg aaattctttg caacagcttt tgcgatatcc cacgaagtta cgataccggc
     5581 gagctgcccg ctcactgaca aaaccggcag gtggttgacc tccagcgtga tcaatctccg
     5641 tgccgtcact gctgttgtag ctccttcctc aatggtcggg acatcacgga tcatgacatc
     5701 cccgacaaca gttcttgaga ggaactgggt gatcaggtaa ttgatctgac cggattccag
     5761 taagaatgca tcatgcccgt agttggaccg gatctcgctg taccgcacct cgcattcatt
     5821 tgccgtcagt gccgagacga tctcctgcga ctggtaaggg ggatacagcc agtcagaaga
     5881 tacagagata atgagaaatc cagcacgcac tcccgcaagc ccttttgcaa gcgaaccgtc
     5941 ttttgtaagg tcgaagtaat cgatcgcttt tgtgatgtac aggtacgagt tggcatcgaa
     6001 ccgccgggta aacgtatcgc cctggtggtg gagatagctc tcaacagcaa aatcagtaga
     6061 gaaattaaac ccgatccggt cctttgcctg gagtgagcgg ccgaactttg catgcatcga
     6121 ttcgtcagag aggtacgtga tatggccgac catgcgggca agagcaagtc ccctgaccgg
     6181 gccgtgtgcg tcatacgatt tcgtatcata gtaattcccg ccattccatt cgggatcaga
     6241 aatgatcgcc ttccgtccca cttcgttgaa cgcgatctgc tggggtgtgg agtaacccgt
     6301 tgcagctatt atgatcgctt ttctcaccgt atccgggtag ctcacggtcc attggagcgc
     6361 ctgcataccg cccatcgatc ccccggccac tgcgtagagc tgcgggatgc cgagatggtc
     6421 gatgagcagt ttctgggcat gcaccatatc ccggatcgtg atcaccggaa aggttgcgcc
     6481 gtaaggcttt ccggttgccg gattttccga cgacggaccg gtcgatccct tgcacccccc
     6541 aatcacattt tggcatatca cacaataccg gtcggtgtcg aaagctttgc ccgggccaac
     6601 aaccgcgtcc caccagcccg gtttatcatc acctgcatga aatccggcaa tatgtgaatc
     6661 gccggagagt gcatggcaga ccaaaattgc attattccgt tcctggttga gcttaccata
     6721 tatctcataa gcaatagtaa gggaaggaag cacatccccg ctttccagta taagcggaga
     6781 aggatggtga taaaagtggg ttgttacggt gcccactgac cctttaatca tctacacaca
     6841 ccgtctttgt aaaacaaaag agttccgcga caatgtttcg gatgtttttg aaaggatccg
     6901 gttgttaggg atagcatcat accactgcca gtgcctgctc aaggtctgca ataatatctt
     6961 cgatatcctc ggttcctaca gagagccgga ttgcatcagg agtaacgccg gtctttttct
     7021 gttcttcaac agtcagctgc tgatgggtgg tggatgccgg gtggataacc agactcttgg
     7081 agtccccgat gttggcaagg ttggagaaaa gcctgaggct gtcaataacc ttcaggctgg
     7141 ccttctctcc tcctttgaca ccaaacccga caaggggacc aaagcccccc acgagatatt
     7201 tctttgcgag tgcatggctc tggtgcccgg gaagtccagt ataattcacc catgccactt
     7261 tcgggtgtgt tgttaaaaat tccgcaactg ccattgcatt ccgggaatgg cggggtacac
     7321 ggagatggag agtttcaaga ccgagtaaga agagccaggc attgaacggg ctgagtgcgg
     7381 caccggtgtc ccggaggagc gagagccgga tcctgaagat aaaggcaatg ttgccggcac
     7441 cggggaagtt gctgaatgca tcccagtatt tcaggccgtg atagcttgga tcggggtccg
     7501 tgaattcagg gaatttcccg ttgttccagg caaactttcc tgaatcaaca atcaccccgc
     7561 caatagagtt cccgtgcccg ccgatgtact ttgttgctga gtggacaaca atatccgcac
     7621 cgtgccggat cggtcggaca agcccgacac ccgttgtatt atctacgacc agcggtatcc
     7681 cggcgtcgtg ggcaatcttt gcaagggtta caaagtccgg aatatccagt ttcgggttgc
     7741 caatggtttc tgcgtatatc gcccgggtct tttcagtaat ggcgttctta aacgctgccg
     7801 ggttcgttga gtcgacaaag acaaccttcc tgccgaattt cgggaaggta tagttgaaga
     7861 gctcatacgt accgccatac agattgtctg ctgctacaat ctcatctccg ggacgagtta
     7921 tgtttaagac cgagtaggta atcgcggcct gtccggaagc taccgcgaga gctccggtcc
     7981 ccccctcgat tgctgccatc cgcttctcaa aaacatctgt ggtcgggttc atgagccgtg
     8041 tataaatgtt cccgagctca cggagcccga acaggtttgc cgcatgttcg gcactcttga
     8101 acacatacga tgaagtctgg taaatcggta ccgtacgaga cccggtggtc gggtcgggta
     8161 cctgccctgc atgaagggct gttgttccta aacggaattc tttgtctgcc atgagatttt
     8221 cctcatttgg taacgggtat ttcgtatttt tctgcgattt cggtaaacac atcagcgaga
     8281 tagtggattt gcctgtcatt gaggccatac gtattgagtt tccaggtccg cgttgcaccg
     8341 gcgaactccc cgactatgcc acgggacgac agttcatcgc tgaagtagaa ccctttacgt
     8401 ttgtgcgtct ttgcaatggt gtcgaaactt ccggtggtat cgattttggt gagggtgtgc
     8461 ttacgcggat actccgagag cacggtacta ccggagatct tcaggagccg gtcaatgaag
     8521 taattcgacc tcttcacctg ctcatcccag tgaagcaccc gttctttcac ggtcgggaac
     8581 gatgccatca tggagagcag ggtgcccccc atcagcgtgc atccgagcat ctcaacttct
     8641 tttataccga actttcgttt ggtgaggtcg ccgaccatcg tggtggtgcg gagtgccttt
     8701 ggtatccatt catcggtcat cgcaagtacc ccggacggtg cgaccgatgc catactcttg
     8761 tgtccggagc cgacgacaaa gtcagccccg atcttcttcc cgtcaaccgg cattacgccg
     8821 accgtgtatg ccccgttata gaggaaggga atatcatact ggtgggctac tttgccgatc
     8881 ccggcgacct cgtgctcatt ggcgaactgg tagtcgaagt ggtcgattat gacgagcacc
     8941 gggagtttcc cggtctctgc tttgacctct tcgatcttct gtgccattgc ttcagcggta
     9001 acgatattct ttgcgttgag cggcacttcc ttcacaaccc ccccggcatt ttcaacggca
     9061 aggaactcgg tgtagtgggc aagggctgat acaatcacgc tgtcgtcttt tccgaccagc
     9121 gtgccggcaa ccgcctggaa cccccgccgt gcaccgggca cgacacgggc ctggtccata
     9181 ttgagccatt gggcaagtcc ggcgtggaag tccgcaattc ccggctttga gatcttgtcg
     9241 agccggaacg gcttccggca ggcgtcacac gttgagtaac catcaccata ggagatgatg
     9301 gctttccgtg cctcggtcgt taacctgccg gctgcctgga tgggatggat gttgatgtac
     9361 tgctcctccc gcgacctgat gtcgatcgag ccggcaatct ttgtcacttt tggtgagccg
     9421 gtccctgctt cgagatcggc aacgattgcc ttaagatcgg caatccgctc tttgaatgcg
     9481 gcctcttcac ccgcatcgaa cccggttggc agagactcac ggaagagtcc tcttatctct
     9541 tcgagtgcga agagtgcttc gaatgttttc tggaccctga tactcatagt gtctccaagc
     9601 caaggcccgg aatcgaaccg ggttgtagtt gatctgcagt caaccgcgta gccactccgc
     9661 caccttggca ttttgtgata attcacaatt gtgattattg accataatag ataacaattg
     9721 tgaatattta taggtttcga aaccggcagt tttctgatat taatgcgcag tgtacatttt
     9781 ctcttcttca cgcgtctgac tcatggcgcg ggaaataaaa aacagaaccg gggccggcat
     9841 tcaattcatg acaaaaattc cgtggtgtcc caaagttttt aacccgaccg tgttccagtc
     9901 tgattatgca cagtaccccg tggagcacag gtattaccat ttgtggagca ggagctctcc
     9961 tcttagtcct gatcctggta tcaggatgca ccggcactgt tcaacctcct ttagtaaccg
    10021 gaccccctgg aatatcaccc aacgataccg accggtcctg gatggatgtt ccccttatgg
    10081 acctgcaggg aaaaggtaat ttctctcccg ggtcgtttgc gggaaaatcc gtagtgctcc
    10141 ttgttgtatc ggatgcatgc ccggcctgtg tcagccagct cagccgggag attggcgaga
    10201 tcgaaggtct cgcccgggtg caggacggga gtatcgtgtt tgtcgcgctg gatttagatc
    10261 caaccgggga tccgggtttt atcacaaaat atcatgacca gttcaatttt accgggtaca
    10321 ccgcccgttc atctgaagag atgaccctga aactgttcag ggaactggga ccgtttgcta
    10381 tcgataccga agcgatcccg gtaatcctta tctgcccgga taggcacgag gtactgcttc
    10441 cgcctggctt taagcccaca gacaagctcg atacccgtta tacgcaagag tgctgaacaa
    10501 tggaaacttt tacccttttc tggctctcgt tccttgcagg tatccatgcg tcagccggtg
    10561 ctccctgcgc tttggtcatg tacccgggat atctcgcatt cctcgcaggg aaatccggtg
    10621 atgagaggta caggcatgaa ccattcacgc ttggtatcct tgtcgccatc ggggtaatcg
    10681 ctgccatgct caccgggggg attctcttct ctttcgcagt cgcagaagga ggcggggcag
    10741 tgcgaaatct catcgccccg gccgcgttta ttcttctctt ctttttctcg ctcttcataa
    10801 tctttgacat caatttcggg aagattccgg tagcaaatcc catcccaatg gtgaaaaatc
    10861 cgcttgcagc tgcattcttc ctcggtctgg cctttggtat catcatcctt ccctgtaatg
    10921 ccgcagcagt cgttgtcctt cttgctcttg cagcaactgc gaccggtctt tttgagggac
    10981 tgggtgcgtt cctctcattt ggcctgggga tcaccctgcc cctggtaatt cttgcgggtc
    11041 tctctcgtgc ggccgggcag caggttatgg agtacctgac ccggcaccgg agagctatcc
    11101 gtctgaccgg tgggataatc atgctgataa tctcggtctg gtacctggcc ctgctgtttc
    11161 ttccgcagat gtcccgataa ttacagcctg ccaggaagac taaaaaccca ctactactat
    11221 gtgtggagag acaattacgg gcaagagagg aaaaaaagat cttatttttc cagttttttc
    11281 atcagccacg ccatattcgt gcccagtgtc ttcatggtca aaagcccctc ttcatccttc
    11341 ttcacctcgc cgggtccaag cccgataccg acattccagt aggatgagcc ggggacaatc
    11401 atctggctga tgaagaagaa atggttcatg gtatcaaacg cgtggattgc cccggcacgg
    11461 cggacagcaa ccaccgaagc cccgaccttg cgccggaaca tgtcgttgtt tgcccgtgcc
    11521 acaaaacccg accgctcgat cagggccttc atattcgccg aaatatccgc aaaataggtt
    11581 ggggatgcaa ggatgatccc gtcagcctcc agcatctttt caatgcactc attgaggata
    11641 tcactgacga caacacaccg tttgtcttta ttctcaaagc atttcccaca ggcgatgcac
    11701 ccggagattt tctttcctgc aagctgcacc agctcggtct cgatctttgc cttttttaac
    11761 tctttgaaga ccgtgttcac caggatggcg gtattgccgt cctttctggc actgccgttg
    11821 aatgcaatta ctttcataag ctcctcgtcc agagagtttt attccggatg ttatagaatc
    11881 atcgccggtt ttttccggta attgcgtttt ctcctgtgcc aggagggata ttttaacggt
    11941 aaacaaccgc tggttcatcc ataatattct atatagttcc tgctaaaaat ggattacagg
    12001 cgcactccta tggccgaaga tgagttccag ttcatggctg tcgacaaggt gacagaaacc
    12061 ctgcatacct cgcttcaggg actcacgcca gaagtggttg aatcgaaaag ggcacgctat
    12121 ggcccaaatg aggtagtgcg ggagaaaaag cgggcagcct tccgccagtt tgccggccag
    12181 tttaaaaacc cgcttatcat tatcatactg acaggatgaa tgacagaata catgagattg
    12241 atgtaaccgg caccattatt ctaagaagtg gtgggtcagg ttcatcacat cccggtaggg
    12301 aatgactccg acaagccggt tcttttcatc tgttaccgga agagcacgaa aatcatatcg
    12361 tgcaaaactc tgcgatgcct ctttaagggt gctatcggtg ttcaggctga tcacgttttc
    12421 gatcatgata tcttttaaga gggcccggtc atcggcctgg agtaactctt ttaagtcgat
    12481 aaccccgacc aggacatcca gatcatcaac gacatagata tacatgatca cgtccttgtc
    12541 acgggcatgc cgcgggtaat cgttctggac atattcaaca gtcgtttgtg gggaaaattt
    12601 caggaacttt ctggtggtat aattgatgac cttctcttcc tgttttccga tgatggactg
    12661 gatttttttt gcgttctcct tgttgagaga ttccaggatg atcctggctt cagaagacgg
    12721 aagtacggaa a
//
LOCUS       DASRLV010000203         5676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_640349,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000203 DASRLV010000000
VERSION     DASRLV010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5676
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640349"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..699)
                     /locus_tag="VFC43_07765"
     CDS             complement(187..699)
                     /locus_tag="VFC43_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HZK31108.1"
                     /translation="MNLEIFDKMDDADKHQYLEFLLWHYRVVDAFWFINITEKFGQPE
                     AETINEQVWGRVAGMAAKDIVRRFSITEKGLTGFIRALRHFPWAMIVGYTIEERDNEV
                     ILSVPSCPTQEARLKRGLGEYVCREMHRAEFTSFANEVDSQIVVECIFAPPDPHPEDM
                     HCKWRFYLRS"
     gene            916..2259
                     /locus_tag="VFC43_07770"
     CDS             916..2259
                     /locus_tag="VFC43_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HZK31109.1"
                     /translation="MMQDYEHELSQIKDLLKQNPGGMSVTNISKALNKNKNTIGRYLD
                     ILLISGQVDMRSYGMAKVFTLSQRVPLSAMLRYSKECIMVLDNESRIIDVNDNFLKLL
                     NLTREDTVGKNIKFLRPPEVDVHELLETLATGSGEKEDIVTFQVKDEGEKIFKQKSVP
                     TVFEDGTKGFTLILEDITKQVLAERKIRENEQRFRMMAENIHDGLIIMENNIVVFVNR
                     RIAEITGYSFEELWKMNPLSIIDPEDKETVASQIKKMDKKDQGLAELRMWIRRKDGQR
                     RHTSARISSLLHADIHYVFIILTDITEIKTHEAALKESEHRFRMMAENIQDGLIIVEN
                     GNFVFANHRIANITGYSNKELIRMKSADLVSPEDHEKIEEMVKNTQPDSEKPGEFTLW
                     INRKDGTRRCILARITAARHHNTVSTYCTMTDITESIEREQALRDRIAALQKFIG"
     gene            2609..3811
                     /locus_tag="VFC43_07775"
     CDS             2609..3811
                     /locus_tag="VFC43_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HZK31110.1"
                     /translation="MSGSPELSAYISGSNEVSPGETVQLKVMIENKGVNEFKFVQSGI
                     IDREDLPNTAKFLTVSLASGDAPVTIKSDPQMLGDLKGSSTTNCVITMKIDPNAAAGS
                     FSLPLIMNYSYLYEADQYGVDTIQYYYKEKNEIFSLPIKIKAELKLSVLSAEAEHLNA
                     GTEGFVNLTVKNIGHENGKNAIIKIVRNDMSPVLPVSGSMFIGDFSVNQVISSRFKVS
                     ISSDAEEQTYPLDVQVSYENGEGDQITSDVETIGLQVGKKIDFNIISEPDTVTPGLKK
                     VITVQYTNTGGAPAYNVQARISAVDPFTSNDDTAFLGTLEPGETREAVFEISVDKTAT
                     LKEYALDSEVRYRDSLDNSLISDPMKVRIVVKKEDSILSNPFILAGGIAVIILIGYLV
                     YRQRTNKN"
     gene            3936..>5676
                     /locus_tag="VFC43_07780"
     CDS             3936..>5676
                     /locus_tag="VFC43_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZK31111.1"
                     /translation="MIERIFEALAHVINRHPRRVAAGIGIILIIALFGMTLITMETGN
                     DTYLEKDSSAGILNKHYTDTFSTNVLILIVETSDPLNPQVLSYLDELERNINQQENVL
                     GSASVADLLKSANGGVLPQSKGEIDRIVARIPPEVRATAVPSNVMTLVQITLSQGMSE
                     DAKTTTLKNVESLVAQNSPPPGVKISVSGSAAFSEQMKTEMGSSMGVLIGGAMVLMVI
                     VMGILFAYASHRFLPVLFVGIGLTTAMGFMGLAGIKLNMAVLGAFPVMIGLGIDYGIQ
                     FHARLDEEARKGPLDKAVTVMITRTGPAVMYAMLATCMGFLAMFISTVPMIRSFGLVA
                     MIGVMSCFVISLIGIPTIAHLVNYTPKQQKTELCYAVGEGACDYLPSQTHGAVSGAGQ
                     KKNNGSYGQFLTDISVKIAKNPIPILLIAGVIALIGFQIDPQIPIETSENAFVPSDMP
                     AKVQMDKVTGILGSTSSADFIVQGSRVTDLDTVRWMKEFQDYELAHHTEITRATSIVT
                     YILAYNGGVMPQDQNQLNAVIEKIPADVKKPYLSGSMEGVIRFNTVKLEMTSLNGLKI
                     QMEKDIKFLQPPTG"
BASE COUNT         1686 a         1308 c         1242 g         1440 t
ORIGIN      
        1 tatcaagggc gaaaaagaga aacgcaatcc cattaaggac taaaaaaact gccaaaaata
       61 taaaaaaaaa tgtaattttt atcatcccgt gctaacctgc tttattttct gccacattat
      121 catttacacc agaattctat aatgccgaga atccgaatca tttcttaaaa aacattgacc
      181 atgaacttac gacctaaggt agaaacgcca tttgcagtgc atgtcctcag ggtgcggatc
      241 tggcggggca aagatacatt ctacaacaat ttgggaatct acctcatttg caaaactggt
      301 aaactccgcc ctgtgcattt cacggcatac atattctcct aatccccgtt ttaaccgggc
      361 ttcctgtgtc gggcatgagg gtaccgagag gatcacttca ttatcccgtt cttcgattgt
      421 ataaccaaca atcatcgccc aggggaaatg acgtaatgct cgtataaacc cggtcaggcc
      481 cttttctgtg atactgaacc tgcggacaat atcctttgct gccatgcctg ctactcgccc
      541 ccatacctgc tcattgatcg tctctgcctc aggttgcccg aacttttcag tgatattgat
      601 gaaccagaat gcatccacaa cacgataatg ccagagaaga aattccaggt actggtgttt
      661 gtccgcatca tccatcttat caaagatctc aaggttcatg ccacccacac atatcaccgg
      721 agtaatgaat gactgattgt attcacagaa tctcttatga atctggtgtt ctggctgttc
      781 cacccttaaa ttttgagtga aattgtgcca tgagacaaac atttattaat agttttgttc
      841 tattaactat ccatacaaat tgtcaataat actttacttt gccattgccg aacagaaaac
      901 agacgtgata tccagatgat gcaggattac gaacacgaac tctcccagat taaagatctt
      961 ctcaagcaga atccaggagg gatgagtgtg acgaatatct caaaggcact caataaaaac
     1021 aagaatacta tcggaagata tctggatatt ctcctgattt ccggacaggt ggatatgcga
     1081 tcctacggaa tggcaaaagt attcacgctt tcacaacgcg tcccgctatc ggcaatgctc
     1141 aggtactcaa aagaatgtat tatggtactg gacaatgagt cccgcatcat cgatgtgaat
     1201 gacaatttct taaaactttt gaatctcacg cgggaggaca cagtaggaaa aaatataaaa
     1261 ttcctcaggc cgccagaggt tgatgttcac gaattactgg aaacacttgc gaccgggtcg
     1321 ggagaaaaag aagatatcgt tacattccag gttaaagacg aaggagagaa gatcttcaag
     1381 cagaaatctg taccaactgt ttttgaagat ggcacaaaag gttttaccct tatccttgag
     1441 gatatcacca agcaggttct tgcagagcgg aaaatccggg agaatgaaca gcggttcagg
     1501 atgatggctg agaatattca tgatggcctg atcatcatgg aaaacaatat tgtcgtcttt
     1561 gtaaacaggc gtattgcgga aatcaccggt tattcctttg aagaattatg gaagatgaac
     1621 cctctttcta taatcgatcc agaagataag gagaccgtag caagccagat taaaaaaatg
     1681 gacaaaaaag atcaggggct ggccgaactc cggatgtgga tacgacgaaa agacgggcaa
     1741 cgacggcata catcagcccg gatatcttct ttgctgcatg cggatatcca ctatgttttt
     1801 attatcctga cagatatcac ggaaattaag acccacgaag ctgcattgaa agagagcgaa
     1861 caccggttca ggatgatggc tgagaatatc caggatgggc tcatcatagt ggaaaacgga
     1921 aattttgttt ttgccaatca ccgcatcgca aatatcaccg gatattcaaa caaagaactt
     1981 atacggatga aatcagctga tctggtatct cctgaggatc acgaaaagat cgaagagatg
     2041 gtaaaaaata cccaaccgga ttcagaaaaa cccggagagt ttactctttg gattaaccgt
     2101 aaagatggca cccgtcgctg tatcctggcc aggatcactg ctgcccggca tcacaacact
     2161 gtctccacct attgtaccat gacggacatt accgagtcaa tagaacggga acaggcactc
     2221 agagatcgca ttgcagcact ccagaaattt attggttaat caatttactt ctccggattc
     2281 actatctatt tttattgttg ctattgcatc acgaattccc cgtatattct caccccgaaa
     2341 atagtttatt ataattatgt ataatctata aacaataagt ttatatatta ttgatttaaa
     2401 catgaataaa ccattcctaa tcaagaggac attcatgact aatattcagc aaaaatctgc
     2461 cagcagaaac gtgagttctg cattatctac gaaatttgcc ttcctatgta acacccggtt
     2521 cttattcatc tgtctcgttg tcatcacatt gtccgcattg gtcatattcc cagtgcaggc
     2581 agcaacggta gcaacaggta caaaatacat gtcaggaagc cctgaactgt cagcctatat
     2641 atccggctcg aatgaggtaa gcccaggaga aacagtccag ctcaaagtga tgattgaaaa
     2701 caaaggggtc aacgagttca agtttgtcca gtccggtatt attgaccggg aggatctccc
     2761 aaacacggca aaattcctga cggtatcact ggcatccgga gacgcaccgg ttactatcaa
     2821 atcagatccg cagatgcttg gggatttgaa aggtagcagc acgacaaatt gtgtaataac
     2881 catgaaaatt gatcccaatg cggctgcagg atctttcagt cttcctttga ttatgaatta
     2941 ctcatatctg tatgaagcag accagtacgg tgttgacaca atccagtact actacaaaga
     3001 aaaaaatgag atcttcagcc ttcccataaa aatcaaggcg gagctgaaac taagcgtact
     3061 ttcggcagag gccgagcacc tgaacgccgg tactgaaggc ttcgtcaacc tgaccgtaaa
     3121 aaacataggt catgaaaacg ggaaaaatgc catcatcaag attgtccgta atgacatgag
     3181 tcccgttctg ccggtctcag gaagcatgtt tatcggggac ttttcggtca atcaggtcat
     3241 cagcagccgg tttaaggtat cgatctcaag cgacgcagaa gaacaaacct atccacttga
     3301 tgttcaggta agttacgaga atggtgaagg agatcagatc acatcagatg tagaaaccat
     3361 cggtcttcag gtcggtaaaa aaatcgattt caatattata tctgaaccgg atacagtcac
     3421 tccagggctg aaaaaagtta tcaccgtcca gtataccaac accggtggag ctccggcgta
     3481 taatgtccag gcccgaatca gtgcagttga tcctttcacc agcaatgatg atacagcatt
     3541 tctcggaacg cttgaacccg gcgagacacg ggaagctgta ttcgagatat ccgtagacaa
     3601 gactgcaacg ctgaaggagt acgcccttga ctctgaggtc cggtatcgcg attcactgga
     3661 taattcactg atctcagatc caatgaaagt ccggatagtt gttaaaaagg aagactccat
     3721 tctaagtaac ccatttatcc tggctggtgg aattgcggtt atcatcctga ttggttacct
     3781 ggtctatcgc cagagaacga ataaaaacta acaggtgtgt tcatgtcagt accacctggc
     3841 ggatgctcac acgggcacat atttcggaac cgggaaggca tatccttcct tttcatcaat
     3901 ttcctgctcc gatcaatgga gagagaagag aaaaaatgat cgaacggata tttgaagcac
     3961 ttgcgcatgt catcaaccgg catccaaggc gtgttgcagc tggtatcggg attatcctta
     4021 tcattgcctt attcgggatg acactcatca ccatggagac cggtaacgat acctatctgg
     4081 aaaaagattc aagtgcagga attctcaaca aacactatac tgacaccttc tcaacaaatg
     4141 tgcttatcct tattgtggaa acaagcgatc cactcaatcc gcaggtcctc agctatttag
     4201 acgagcttga acgtaatatc aaccaacagg agaatgtttt aggatctgca agtgtcgcag
     4261 acctcttaaa aagtgcaaac ggtggcgtac tgccccagtc aaaaggcgag atcgaccgga
     4321 ttgttgcccg tataccacct gaagtaagag caactgccgt cccatcgaac gtcatgaccc
     4381 tggtacaaat cacgctctcg cagggaatgt ctgaggatgc aaagaccacc acgctgaaaa
     4441 atgttgagag cctcgtggca caaaacagtc ctcctcccgg tgtaaagatc tctgtttcag
     4501 ggagcgctgc attttcagaa cagatgaaga ccgagatggg atcgtccatg ggtgtgctta
     4561 tcgggggtgc aatggtactc atggttattg tcatgggcat cttgtttgcc tatgccagcc
     4621 accgtttcct cccggtgctc tttgtcggga taggcctgac gaccgcaatg ggattcatgg
     4681 ggcttgccgg gattaagctc aatatggcgg tacttggtgc tttcccggtt atgatcggac
     4741 ttggcatcga ttatggcatc cagttccacg cccggcttga tgaagaagca cggaaaggac
     4801 cgcttgacaa agccgtaacc gtaatgatca cccgtaccgg tcctgcagtc atgtatgcaa
     4861 tgcttgccac ctgcatggga tttctcgcga tgtttatttc gacggttccc atgatccgtt
     4921 cgttcggcct tgttgctatg ataggtgtca tgagctgttt tgtcatttca cttatcggca
     4981 ttcctacgat tgctcacctc gtcaactaca cgccgaaaca acaaaaaact gaactctgct
     5041 atgcagttgg tgaaggcgcc tgcgattatc tcccctcaca aactcatggt gctgtgtccg
     5101 gcgcagggca aaaaaagaat aacgggtcct acggccagtt cctcacagat atttcggtaa
     5161 aaatagcaaa aaatcctata cccattctcc ttattgctgg agttatcgcc ctgatcggtt
     5221 tccagatcga tccccagatc cccatagaga caagtgagaa cgcttttgtt cccagtgata
     5281 tgccggcaaa agtccagatg gataaagtga ccggtattct tggctcgaca agttctgctg
     5341 atttcattgt ccaggggagt cgtgtcacag accttgatac agtgcggtgg atgaaagaat
     5401 ttcaggacta tgagctcgcc catcatactg aaattacccg tgcgaccagt attgtcacct
     5461 atatccttgc atacaatggg ggagtaatgc cacaggacca gaaccagctc aatgctgtga
     5521 tcgaaaagat cccggccgat gttaaaaaac cttacctgag cggttctatg gagggggtca
     5581 tacgtttcaa cacggtcaag ctcgagatga catctctgaa tggcctcaaa atacaaatgg
     5641 aaaaagatat taaatttctc cagccgccga caggaa
//
LOCUS       DASRLV010000204        15886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_321526,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000204 DASRLV010000000
VERSION     DASRLV010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 15886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15886
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321526"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VFC43_07785"
     CDS             <1..439
                     /locus_tag="VFC43_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31112.1"
                     /translation="PQLRGPTEEPPETGGRQQDLPDHGLIMIIAFFLIAAAVAIHSQQ
                     KKHPTWKQPADILLVVGAILTYSGTLYQSYLISLRNGQHLTGTHATLGLIAIVLLTCI
                     VVLWNRNEDKNKNQEQKNIWIVFLWVVTTTLCLVLGLRSVGIV"
     gene            548..2869
                     /locus_tag="VFC43_07790"
     CDS             548..2869
                     /locus_tag="VFC43_07790"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HZK31113.1"
                     /translation="MSWSDSFQEQYPHLVRRLRFLIFMQIFFGILFLIISPVHAAADT
                     NSVTDPHSVWVKSFEQTGNAGSYTVIATSDGGSVTSGSSISSPGLSDIYVIKTDPDGN
                     KIWDFSEKASPYEIHSIVETQEKGYVIAGTANSTVMNGIFLLKLDPSGKKTWTHTFNK
                     SEYYSKNYISMTRDGGFIVVGSMFGTNSSPSSLWDVYILKTDSEGKELWTGFFMGQDN
                     DFASSVSQTEDGGYIIAGTTESYVNGSGPHSFLLKMTEFGNKEWFTTYENGPGNEKPS
                     VIQTPDGGYILLATSLPNTSNQQSQDLFLVRTDDRGKELWNKTISGMGGAGVKLLIQE
                     AESIAVVTGSYGVSEPEKTREIIVINFNMNGIEERNRTFSIGKPLDVQDISVAKGGYF
                     IAGVGSDEGNLQKKDLTIIKVLDTPFDKEGVVKNSFELTIVAKDIKTGSFIGGANVYI
                     DGNSVGSTSDQDGKQILRDVEGGSHTIRVAKTEYDETTRYVYLTEKRQVSVTLGKSKV
                     IPLRIHGSTDEKIDVVFVASETSFNCNSKTKIRTEIYSGDQQRFIGDVNNKIDHVFLS
                     LDTLTTGSQGLPKDFRERFNFYYYWDPISFADAFDGCAGSLPQKFWNDAPFTDVAIIL
                     YPSYEGFYSGSPCEPNGCANGLGPGSGSWLKAPADSPMIFLHESGHVVFGLIDTYCGD
                     TYYVENSPFPNVWDSENACKENARQESWDSASCRQIFKPAGSRSKDPCVKNFWRVDPD
                     PDIMGSGAYSGRFGNASTLHIRLIFDTINRWQK"
     gene            2866..3462
                     /locus_tag="VFC43_07795"
     CDS             2866..3462
                     /locus_tag="VFC43_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31114.1"
                     /translation="MKHFVIACLIFLVILQGTGIAAAQGQPDYSKIVIVNLNISKSGF
                     TEKSVEMRYGHPPNLETRSGDFKGMIKSADGSTIREFDLWDPRYQLGDVLEKDNESSG
                     YLSGYLTYSDNADLTLILPYYENQTTFELIDKKTGVLLKEVNMSPAIKDFQSAYPKDP
                     ESVSIPPIKIDSSVIYLIIGIVLSILIIGMILSMARRK"
     gene            complement(3514..4110)
                     /locus_tag="VFC43_07800"
     CDS             complement(3514..4110)
                     /locus_tag="VFC43_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HZK31115.1"
                     /translation="MAGKIIGLLGSPLTEGNTALLFERALKGAEDAGCSVEKIVVTNL
                     DFQACQEMMFCKEHETCILDDDLQAIYPKFREMDGLIIATPVMTMGIPGKLKSFIDRF
                     QVFFMAKYVRKKPFISKEKRPIRKGLFICISGMNIPEVFVGSKLTITAFFDIIDCQYS
                     DELLISDMDTIKDIRLHPDLLEAAYKKGLALGKSLLSS"
     gene            4245..4475
                     /locus_tag="VFC43_07805"
     CDS             4245..4475
                     /locus_tag="VFC43_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31116.1"
                     /translation="MTEQKTYTAGQKVGAGKYVCIDCGKELTLDKSEQDLRKCPACAC
                     EEYQCFPMTHIRPDVKTPEDARNPPKRGKSNQ"
     gene            4590..4718
                     /locus_tag="VFC43_07810"
     CDS             4590..4718
                     /locus_tag="VFC43_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin FeS4 iron-binding
                     domain-containing protein"
                     /protein_id="HZK31117.1"
                     /translation="MVNVSKEGQVFRCEICGNVVIVKEAGGGELICCGEPMQLESD"
     gene            4723..5127
                     /locus_tag="VFC43_07815"
     CDS             4723..5127
                     /locus_tag="VFC43_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HZK31118.1"
                     /translation="MAIKKTVAKKAPAKTTPAKKGSDKKLQDLEKKIGKVPKFFRELT
                     TKEPEMFNLVMRFEQHIWDDGKLSKKTKKLIAIAIAAALRDQHAVRAQLAGAKNLGVT
                     KAEVEEALRVTFLLSGMPAYVYGKAQLDEVMP"
     gene            5150..5794
                     /locus_tag="VFC43_07820"
     CDS             5150..5794
                     /locus_tag="VFC43_07820"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZK31119.1"
                     /translation="MEDDICITFPILGEPAPDFEAETTQGPIKLSDLKGKWVVMFSHP
                     ADFTPVCTTEFMAFTNIYDELKSLNVNLIGLSVDSISAHLAWVHDIKEKMGVTVPFPI
                     IADLTMKVAKKYGMIHPGQSSTAAVRCVFFIDDKSIMRAMIYYPLQNGRFMPEIIRLV
                     KALQTTDKFKVSTPANWQPGDKVVVPPPKTTAEMDKRMTEGYECKDWYLCFKKI"
     gene            5868..6482
                     /locus_tag="VFC43_07825"
     CDS             5868..6482
                     /locus_tag="VFC43_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HZK31120.1"
                     /translation="MPIKILAFAGSPRRYGNSETLLDWVLESMIADPEVVVEKVPLDE
                     ANINSCKGCNACEKLNKCVQRDGMDIYHDKIIAADIVLLSSPIYCMGIASQPKALIDR
                     AQVFRSRKYVLKLPIVPPERKGKRLGVFLSTAGQTWSFVFDAAVPSVKCFFHVIDIKD
                     ADISYLMINGVDEKGAIDLHPTARTDASNLGKSLIAEIKKRLAA"
     gene            6488..7093
                     /locus_tag="VFC43_07830"
     CDS             6488..7093
                     /locus_tag="VFC43_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HZK31121.1"
                     /translation="MTITVLGISGSPHRHGNTETLLDSFLEGARAGGGSVEKVVLKTL
                     QYSPCRGCNACHKTGKCIVEDDAVMLYEKILASDCLVVASPIYTMGITAELKGFIDRQ
                     QYLWARKFILKTLFFSDDHIKRHKGIFISTAGLGWENVFDGAFPVITALFNTTGFEYY
                     DNVIANDMDRFNGIKNHPTALKEAFDKGEKVTGIIKKIQKG"
     gene            complement(7143..7781)
                     /locus_tag="VFC43_07835"
     CDS             complement(7143..7781)
                     /locus_tag="VFC43_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M50"
                     /protein_id="HZK31122.1"
                     /translation="MLERISRREEADLFLAWMAISLSFAIIKIAPNGILGPIKSVDPL
                     VALMYFGISLLTVGVGFILHEMAHKFVAIRYGYWAEFRKDNSMLLVAIALASLVGFVF
                     AAPGATVIYSNSGSGLTRKENGIISASGPVVNLLLCLPFGVLAFLGSPGLGGLVSQIG
                     IFGVQINAMIVAFNLLPISILDGRKVMAWSIPVFGVLILVSFGVLVGSFYLF"
     gene            7843..8166
                     /locus_tag="VFC43_07840"
     CDS             7843..8166
                     /locus_tag="VFC43_07840"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HZK31123.1"
                     /translation="MVMFIKNLGHVPLMSLETIRQEITKVDAHIIRLIAQRQELAGKI
                     AQVKIHGGLPVHDKQRTSRVLEDVFNQAVESKIDPVAVQKIFEILISMSEERQRGFSG
                     EGNLP"
     gene            complement(8213..9292)
                     /locus_tag="VFC43_07845"
     CDS             complement(8213..9292)
                     /locus_tag="VFC43_07845"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HZK31124.1"
                     /translation="MTDRAESAKPASNVTLANSTITAFYFYGDGCSHCEKVKPIIEDL
                     KVKYPDLDIRMLEVYHNTTNQEMFARMSRQFGISSPGVPSLFMGTTAMIGDTDIKNRF
                     ETMILAERERIASCTSTTTETITPPDPTCSPEALQLTIPVVVASALVDSINPCAFSVL
                     IFLLISIVAIENRRRILMVGGVYIAAVFMFYLFSGAGLFSIVHLSGFSAALSLLGATV
                     AIVLGLVNVIDVIKNRNDFILGIPASKKEMIERYISRASLPAVFVLGVLVGIFELPCT
                     GGIYLAILGLMSRSYTLMEGLPYLVLYNIVFVLPLVLILILVAYGISPERANAWRELH
                     RRKLRLIVGLSMIAVGALIISGWAG"
     gene            complement(9289..9714)
                     /locus_tag="VFC43_07850"
     CDS             complement(9289..9714)
                     /locus_tag="VFC43_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31125.1"
                     /translation="MDNTLKAAAGIFIVILIACFVFFFILPRPSAVPPLSEPNFTVPG
                     NITVYFFYGEECPHCHNVMPFIRNLSIKYPDVNFFILETWHNETNNALSQQLNKKLGI
                     QNAGVPEVIVVGNNTPLVGDRDIPAYLEGVILDQLKKKQ"
     gene            complement(9783..10655)
                     /gene="argB"
                     /locus_tag="VFC43_07855"
     CDS             complement(9783..10655)
                     /gene="argB"
                     /locus_tag="VFC43_07855"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HZK31126.1"
                     /translation="MKREDVLMEALPYIQEFHGKTIVIKLGGHAMVDPVVLENAIRDA
                     VLLHYVGIRVVLVHGGGPEISEKMKALGKESVFVGGLRVTDQETLEIAQMVLVGKINA
                     GIVSLIAKCGARGVGLSGSDGNLIIAKKMDIQRVEVGGVEKDVDLGHVGEIEWIDPAL
                     LNTLLDKSYIPVVAPIAIDRHGGSLNINADTAAGDIAIALKAYKLINMTDVDGVMDAK
                     RTMVYRKLSITEANAMQKSGAIGEGMIPKVGSLLKAMENGVNFAHIINGNIEHNLILE
                     LFTRDGVGTMISLK"
     gene            complement(10655..11812)
                     /gene="argJ"
                     /locus_tag="VFC43_07860"
     CDS             complement(10655..11812)
                     /gene="argJ"
                     /locus_tag="VFC43_07860"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HZK31127.1"
                     /translation="MTFRSICAVKGVKTYGMKEGKFGLAIIRATGTAAGVFTQNLVKA
                     APIELMRNQIKKGTMEAVIANSGCANAYTGKRGYGDAVKMTGFAGSALGLDPEEVGVA
                     STGVIGRYLDLPLIQRQCMEVAKKIDQTPDTEKRAAEAIMTTDTFPKHALVQKESFCI
                     GGIGKGSGMIAPKMGTMLSFMYTDVEIDAKTLSVSLKRATRRTFNRVVVDGDMSTNDI
                     ALCTATGESGRVKPAEFSQALEECCRSLAQQIAADGEGATKLIEVTVKGAQKEESAAR
                     VARTIIESPLVKTAVYGEDPNWGRVVAAAGRAGIGFDPNAVSLWISDGNQQYPLVKSG
                     EIIADLKRAKEAMHGKKVIFILDLATGKEEATAWGCDLTERYVEINGRYTT"
     gene            complement(11813..12307)
                     /locus_tag="VFC43_07865"
     CDS             complement(11813..12307)
                     /locus_tag="VFC43_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZK31128.1"
                     /translation="MLVKDAMTKDPVVCTATTSLRDAVGLMKKYHIGGLPVMEGTELV
                     GMLTESDLIALLESERISDDLWLPSPFEIIEIPIREYINWEKTKHALRNIGDMQAKKV
                     MTHRVVTATEGMEIGAAAALMLKEGISRLPVMRGKKLVGILARADIINAVGSSHSVPK
                     GAET"
     gene            complement(12318..13304)
                     /gene="argC"
                     /locus_tag="VFC43_07870"
     CDS             complement(12318..13304)
                     /gene="argC"
                     /locus_tag="VFC43_07870"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HZK31129.1"
                     /translation="MKIAIIGASGYAGGELVRLLVHHSTAKVTCATSRKLAGTPLDQI
                     HPHLKGFTDLLFENPDIDAIDADVAFLAVPHTAAMTYAGKLLSRGIKVVDLSADYRLP
                     KEIYEKVYGVMHTDYFEAPYGIPELHRTECVNAKFVANPGCFPTGATLAAAPLAKHAH
                     TIIFDSKTGVSGAGDNPSATTHYPNVGDNIGPYKWTTHRHLAEMKQEIAWLGSKAKCY
                     FTPHLVPVNRGILTTAHILLSEPMEQKEVEKLYRDFYKNEYFVRSQKPSLAAVRGSNF
                     CDLMVESEGLRVVAISAIDNLVKGASGQAIQNMNLMCGLNEKDGLVGAGMLP"
     gene            complement(13396..14310)
                     /locus_tag="VFC43_07875"
     CDS             complement(13396..14310)
                     /locus_tag="VFC43_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HZK31130.1"
                     /translation="MLKKFKGCLLGAAIGDALGMPNESTSPNLKKMKYGYRRPYKNHP
                     NEGLNPGQYTDDTQVMILVATLIADGKYNDERYSVALRELYTQGKLRFPDGSVSAVCE
                     HPISKNIPQKGVKSTTSGCIPVAIPFGLVYPDMNEGCERAVRACNVTHTHPAAHAAVS
                     TFVTLIYHTLHDSPDPVEKALEKAQFEDEVLGGKIKKALAMEKEGMDTETALLKIGND
                     VSVFQTLPISIFLIKRYTNPSDLLTVAANAGGNTDTIGLICGAYFGAAKGLDALPADL
                     LEGLEDKQRIDLLGQRLHTLYLRKLGQM"
     gene            14455..15886
                     /locus_tag="VFC43_07880"
                     /pseudo
     CDS             14455..15886
                     /locus_tag="VFC43_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991078.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
BASE COUNT         4318 a         3726 c         3646 g         4196 t
ORIGIN      
        1 cccgcaactc agagggccaa cagaggagcc tccggagaca ggaggccggc aacaagacct
       61 tcctgatcac ggtctcatta tgataatagc attcttcctg atcgctgcgg ccgttgcaat
      121 tcattcacaa caaaagaaac atcccacatg gaaacaacct gctgacattc tgttagtcgt
      181 tggagctatc cttacatatt ccggcactct ctaccagtca tatcttattt cgctccgcaa
      241 cggacagcac ctgacaggga cacacgctac gctcgggctc attgcaatag tcctgttaac
      301 ctgtattgtc gtattatgga accgcaatga agataagaac aaaaaccagg aacaaaaaaa
      361 tatatggatt gtttttctgt gggtcgttac cacaaccctt tgcctcgtac tcggtctccg
      421 gagtgttgga attgtatgac cggtttgttc ccccactata tcgagctgat taccgtacaa
      481 aaacaactca cgggattcat agttgtttta tattgccaga aaaaaagagt gaattggagg
      541 acaactgatg agctggtctg attcttttca ggagcaatac ccccatcttg tccgccggct
      601 tcgttttctg atattcatgc agatcttctt tgggatcctg tttttaataa tcagcccggt
      661 tcatgcagct gctgatacta attctgtcac tgatcctcat tcagtatggg taaaatcgtt
      721 tgaacagacc ggcaatgccg ggagttacac ggttatcgct acaagtgatg gaggatcggt
      781 aacaagtgga tcctccatat cctcgccagg actatcggat atatacgtaa taaaaactga
      841 tccggatggc aataaaatat gggatttttc agaaaaagca agcccttatg agatacattc
      901 cattgttgaa acgcaggaaa aaggatatgt tattgctgga acagccaatt ccaccgtaat
      961 gaatgggatt tttctcctga aacttgatcc ttcagggaaa aagacctgga ctcatacctt
     1021 taataaatcc gaatactact ccaaaaatta tatcagcatg acacgcgatg gcggatttat
     1081 cgttgtcggg tcaatgtttg gaaccaattc atccccctca tcgctctggg atgtatacat
     1141 tcttaaaaca gattcggaag gaaaagaatt atggaccggt ttttttatgg ggcaagataa
     1201 tgattttgcc agttccgtat cccagacaga agatggagga tatattattg ccggtacgac
     1261 agaaagctac gtcaacggga gtggtcccca ttctttcctc ctcaagatga ctgaatttgg
     1321 caataaagag tggtttacca cgtatgaaaa cgggcccgga aatgaaaaac catctgtcat
     1381 tcagacaccg gatggaggat atatactcct tgctacgtcc cttcccaata cctcaaacca
     1441 acaatctcag gatctttttc tggtcagaac tgatgatcgg ggaaaagaac tttggaacaa
     1501 aaccatttca ggtatgggtg gggcgggggt taaactcctt attcaggaag ctgaaagtat
     1561 agcggttgtc actggttcct atggtgtatc tgagcctgaa aaaacccgcg agataatcgt
     1621 aatcaatttt aatatgaacg gtattgagga gaggaatcga accttcagca tcggaaagcc
     1681 attggatgta caggatatat cagttgcaaa aggaggctat tttatcgcag gtgttggttc
     1741 cgatgagggg aatctccaaa aaaaagatct cactattatc aaggttctgg acacgccgtt
     1801 tgataaagag ggagtagtga aaaattcttt cgaacttacc atcgtagcaa aagatattaa
     1861 aaccggttct tttattggtg gggccaatgt gtatatcgat ggaaactctg ttgggagtac
     1921 atcagatcaa gatggcaaac agatactccg tgacgttgaa ggaggttcac ataccatcag
     1981 ggtagcaaaa acagaatatg acgagaccac acgatacgtt tatttgactg aaaagagaca
     2041 ggtgtccgta acactcggga aatcaaaagt gattccattg agaatccatg gatcaaccga
     2101 tgaaaagatc gatgttgttt ttgtagcttc agaaacctca tttaattgta attccaaaac
     2161 aaaaattcga actgaaatat atagcggtga ccagcaacgg tttatcggtg atgtaaacaa
     2221 caaaattgac catgtttttt tatcattgga taccctgacc actggttctc agggtttacc
     2281 caaagatttc cgggaacggt ttaattttta ctattactgg gatcccataa gttttgccga
     2341 tgcattcgat ggttgtgcgg gatccctgcc tcaaaagttc tggaacgatg caccattcac
     2401 tgatgtggca attattctct atccttccta tgagggtttc tattccggtt ccccgtgtga
     2461 gccgaatgga tgtgcaaacg ggcttggacc gggatcggga tcctggttga aagcaccagc
     2521 tgattctccc atgatttttt tgcacgagag cgggcacgtg gtctttgggc tgatagatac
     2581 ctattgcggg gatacctatt atgtggaaaa ttctcctttt cctaatgtct gggattctga
     2641 gaacgcgtgt aaggaaaatg cccggcagga gagctgggat tctgcgagct gtcgtcagat
     2701 ttttaaacct gccggcagca ggtccaaaga tccatgtgtt aaaaatttct ggagggtcga
     2761 tcctgatccg gatatcatgg gaagtggggc atattccggc aggttcggta atgcgtccac
     2821 gttacacata cgtctcattt ttgacacgat taaccggtgg cagaaatgaa acattttgtg
     2881 atcgcgtgtc tgatatttct tgttatcctt cagggaacag gaattgctgc tgcacagggt
     2941 caacccgatt attcaaaaat tgtcattgtg aaccttaaca tcagtaaaag cggttttaca
     3001 gagaaatcgg ttgaaatgcg ttatgggcac cctccaaatc ttgaaacccg gtctggggat
     3061 ttcaagggca tgataaaatc tgctgatggt tccactatcc gcgaatttga tctctgggat
     3121 ccccgttatc agctgggtga tgttctcgaa aaagacaatg aatcttccgg ttatctatcc
     3181 gggtacctca catattctga taatgccgat cttaccctta tccttcctta ttacgaaaac
     3241 cagacaacat ttgaattgat tgacaaaaag accggcgttc ttttaaagga ggtgaatatg
     3301 tcccctgcaa taaaagattt tcaatcggcg tacccaaaag atcctgaaag tgtatcaata
     3361 cctccaatta aaatcgacag ttccgtcatt tacctgatta tcgggattgt actatcgata
     3421 ctcatcattg gtatgattct ttccatggct cgaagaaaat gacgtgtatc ttattggcat
     3481 ttttttaggt tttgatattt tgaatttttc atttcacgag ctcaggaggg acttgcctag
     3541 tgccaggcct tttttatatg ctgcttcaag gagatccgga tgcaatcgga tatccttaat
     3601 tgtatccata tcactgatca ggagttcatc actatattga cagtcaatga tatcaaaaaa
     3661 tgctgttatt gtcagtttgg aaccaacaaa aacttcggga atattcatgc cggaaatgca
     3721 gataaaaagc ccctttcgta tgggtctctt ttctttggat atgaacggtt ttttccgcac
     3781 gtactttgcc atgaaaaaga cctgaaaccg gtctatgaat gattttagtt ttcccggtat
     3841 ccccattgtc atgaccgggg tggcaatgat gagaccatcc atctccctga atttcgggta
     3901 tatagcctga agatcgtcat cgaggatgca ggtttcatgt tctttgcaga acatcatctc
     3961 ctggcatgct tggaaatcca gattggtaac aacaattttt tccacgctgc atcctgcatc
     4021 ttcagctccc tttaatgctc tctcgaacaa caatgcagta tttccttcag taagaggact
     4081 gccaaggagc ccgatgatct ttcctgccat actaacacct taaaaaaaat acgcaatata
     4141 cttttgcctg gtgaattttt tatttttcgg cgtttttttc ttttatgatg cacgaacgca
     4201 actgatatat atctttgacg tgcatgaaag ttttggtgat ttagttgact gaacagaaga
     4261 cgtacaccgc gggacagaaa gtcggcgcgg gaaaatatgt gtgtattgat tgcggaaagg
     4321 aacttaccct ggacaagagt gagcaggatc tcaggaaatg tcctgcctgc gcctgtgaag
     4381 aataccagtg cttcccaatg acacacatcc gcccggatgt caagactccg gaggatgcca
     4441 gaaacccgcc caaacgtggt aaaagcaatc agtaacggta ttatttttct tttcatgcga
     4501 gtacagcaca tgtgactcct gggagtgcct gcaaaatgca gggttcctgc tcccagtttt
     4561 tatctgaaag caaaaagggg gatctctaca tggtaaacgt ctcaaaagag gggcaggtct
     4621 tccggtgtga gatctgtggt aatgttgtta ttgtaaaaga agccggcggt ggagaactga
     4681 tctgctgcgg tgaacccatg caactggaga gtgattgata gtatggcaat aaaaaaaaca
     4741 gtagctaaaa aggcacctgc gaaaacaacc ccagcaaaaa aaggatctga taaaaaactt
     4801 caggatcttg agaagaaaat cggaaaagtg ccgaaatttt tccgggagtt aacgacaaaa
     4861 gaacccgaga tgtttaacct cgttatgaga tttgagcagc acatctggga cgatggcaaa
     4921 ctgtcgaaaa agacaaaaaa actgattgcc attgcgattg cagcggctct gcgtgaccag
     4981 catgccgtcc gcgcccaact ggcaggggcc aagaatctcg gggtcaccaa agcagaagtc
     5041 gaagaggcgc tccgcgtcac cttcctgctc tcaggtatgc cggcctatgt gtatggcaag
     5101 gcacagctcg atgaggttat gccctgatac ggttcagcgg tgagaaacca tggaagacga
     5161 tatctgtatc acctttccca ttctcgggga accggcgccg gactttgaag cggagaccac
     5221 gcagggaccg atcaagcttt cggacctgaa gggaaaatgg gtcgtgatgt tctcgcaccc
     5281 ggccgacttc acaccagtat gcaccaccga gttcatggcg tttacgaata tttatgatga
     5341 actcaagtct ctcaatgtca acctgattgg cctgtctgtg gacagcatct cggcacacct
     5401 cgcgtgggtg catgacataa aagagaagat gggtgttact gtcccgttcc ccatcattgc
     5461 cgacctgacc atgaaagtgg ccaagaaata cggtatgatc cacccgggac agtcatcgac
     5521 ggcagcggtc cgttgcgtat tcttcattga cgacaaaagc atcatgcggg cgatgatcta
     5581 ttaccctctc cagaatggga ggtttatgcc cgagatcatc cggctggtca aagcgctcca
     5641 gaccacagac aagttcaagg tctcaacccc ggcaaactgg cagccgggcg ataaggtagt
     5701 ggttccacca cccaagacga ccgcagagat ggataagcgg atgacggaag gttacgagtg
     5761 caaagactgg tacctctgtt tcaagaaaat ataatgaatt aatcattttg atcagggaaa
     5821 ttgatcgaaa accaaccttt tttagcattg caccccaaaa aaatgctatg ccgatcaaaa
     5881 tcctggcctt tgccggaagt ccccgcaggt atggtaattc cgagaccctg ctggactggg
     5941 tgctggaaag tatgatcgct gaccccgaag ttgttgttga gaaagtcccc ctggatgagg
     6001 caaatatcaa ttcctgtaaa gggtgcaacg cctgcgaaaa actcaacaaa tgtgtacaac
     6061 gtgatggtat ggatatctac catgataaga tcatcgccgc agatatcgtc ctgctttcct
     6121 cacccatata ctgcatgggc atcgcatcac agcctaaagc cttaatagac cgtgcacagg
     6181 ttttccgctc acgaaaatat gtcctcaagc tgcctatagt ccctcccgaa cgaaaaggaa
     6241 aacgcctcgg ggttttcctt tcaaccgccg ggcaaacctg gagttttgta tttgatgcag
     6301 cggttccttc tgtcaagtgc tttttccatg taatagacat caaagatgcc gacatctctt
     6361 acctgatgat caacggagta gatgagaaag gggccatcga tcttcatcca acggcacgga
     6421 ccgacgcctc aaaccttgga aaatctctga ttgccgaaat taaaaagcgg cttgctgcgt
     6481 gagcgagatg accataaccg tccttggaat ttcaggaagc cctcatcgtc atggaaatac
     6541 agaaaccctt ctggatagtt tcctggaagg tgcacgggca ggaggtggat cggtagagaa
     6601 ggttgtactc aaaacgctgc agtattcccc ttgccgcggg tgtaatgcct gccataaaac
     6661 cgggaaatgt attgtggagg acgacgcagt tatgctatat gaaaaaatac tggcttcaga
     6721 ctgccttgta gttgcttcac cgatttacac aatggggatt actgcggagc tcaagggatt
     6781 tattgaccgt cagcaatatc tctgggcacg caaatttatc ttaaaaacac tcttcttttc
     6841 tgatgaccat atcaaacgcc acaaggggat ttttatttcc actgcaggcc ttgggtggga
     6901 aaatgttttt gacggcgcat ttcccgtcat taccgcactg ttcaacacaa cggggtttga
     6961 gtattacgac aacgtgatcg caaatgatat ggaccggttc aatggaataa aaaatcatcc
     7021 gacagccctt aaagaagcgt ttgataaagg ggagaaggta acaggcatca ttaagaagat
     7081 ccagaagggc taatctaccg gatttttgtc tgattcacga gcaaaaagga tagattgggg
     7141 gatcagaaga ggtaaaaaga tcccacaaga actccaaacg agacaaggat cagcacccca
     7201 aataccggga tactccaggc cattactttt ctgccgtcaa ggatactgat gggaagcagg
     7261 ttgaaggcta cgatcattgc attgatctgg accccgaata tgccaatctg gcttaccaga
     7321 ccccctagtc cagggcttcc gagaaaggca agcacaccaa agggcaggca aagcaggagg
     7381 ttgacaaccg gtcccgaagc ggagattata ccgttttcct tccgtgttaa accgcttccg
     7441 gaattgctat agataaccgt tgctcccggt gctgcaaaaa caaaccctac aagcgaggcc
     7501 agggctattg ccacgaggag catggagtta tcttttctga actctgccca gtatccataa
     7561 cgaatggcga caaatttgtg tgccatttca tgaagtatga atccaacccc tacggtaagt
     7621 agggagatgc caaaatacat aagggcaacg agggggtcaa cagattttat cggccccagt
     7681 attccgtttg gcgcaatttt tataattgca aaagacaacg aaatcgccat ccatgcaagg
     7741 aaaaggtctg cctcttcgcg cctgcttatc cgttccagca tgtaaacacc tgatcagata
     7801 gggtgtttat gtgttatctg ctttagtgtt gaggatctct caatggttat gtttattaaa
     7861 aatctcggtc atgtacctct tatgtccctt gaaaccatac ggcaagagat cacaaaagtt
     7921 gacgctcata taatccggct catcgcccaa cgtcaggagc ttgcaggaaa aattgcacag
     7981 gtcaagatac atgggggatt gcctgtccat gataaacagc gaacttcccg ggttcttgaa
     8041 gatgtattta accaggccgt ggaaagcaag attgaccccg tcgcagttca gaaaattttt
     8101 gagatcctga tatcaatgag tgaggagcgc cagaggggct tttccggaga agggaacctt
     8161 ccgtgaaggt catttcagat aaaaagaatt attccggtat ttattatact ttttagccag
     8221 cccatccaga aataattaaa gcccctaccg caatcattga cagtcccacg ataagacgca
     8281 gtttcctgcg gtgcaattct ctccatgcat tagcacgttc aggtgagatc ccataggcaa
     8341 ccaggatcag gatcaacacc agcggcagga caaaaacaat attgtaaagt acaagatacg
     8401 gaagtccttc catcaatgtg tagctcctgc tcataagccc gaggatggca agataaatcc
     8461 ctcctgtaca ggggagctcg aagataccca caagaacgcc cagcacaaat accgcaggaa
     8521 gtgatgcccg gctgatataa cgttcgatca tctctttttt tgatgccggg attcccagga
     8581 tgaaatcgtt cctgttcttt atcacatcaa tgacgttaac cagacctaaa acaatcgcca
     8641 ctgttgcacc cagaagagag agggctgctg agaaccctga caaatggact atcgagaaaa
     8701 gtcctgcccc cgaaaacagg tagaacataa acaccgcagc aatgtaaaca cctcctacca
     8761 tcagaatgcg tctgcggttt tcgatcgcga ctattgagat cagcaaaaag atcaggacag
     8821 aaaaagcaca gggattgatg ctgtcaacca gggcggaagc aaccaccact gggatagtca
     8881 attgaagagc ttccggtgag caggtgggat caggtggagt gatagtctca gtcgttgtgg
     8941 atgtgcagga tgcaatccgt tccctttcag caagaatcat cgtttcaaaa cggtttttaa
     9001 tgtcagtatc gccgatcatc gcagttgttc ccatgaacag gctcggcact cccggactac
     9061 tgataccaaa ctgacggctc atacgtgcga acatctcttg gtttgtggta ttgtgataaa
     9121 cctccagcat ccggatgtcc agatccgggt atttaacctt tagatcctca ataatgggtt
     9181 tgaccttttc gcaatggctg cacccgtcac cataaaaata aaatgcggtt atcgtcgaat
     9241 tcgccaatgt gacatttgag gccggcttag ctgattcagc tctgtcagtc actgcttttt
     9301 ttttaattgg tcgagaataa caccttcgag gtaagcagga atatcacgat cacccaccag
     9361 cggagtgtta ttccccacca caatgacttc agggacaccg gcattctgga tacccagttt
     9421 cttgttcagc tgttgtgata gtgcattatt tgtctcattg tgccaggttt caagaataaa
     9481 gaaattcaca tccggatatt ttattgaaag attccggatg aacggcatca cgttatggca
     9541 atgcggacat tcttcgccat aaaagaaata aacagtaata ttgcccggaa ccgtaaagtt
     9601 cggttcagaa agggggggaa ccgctgatgg ccggggcagg ataaaaaaaa atacaaaaca
     9661 ggctatcagt attactataa aaatacctgc tgcagccttt agcgtattgt ccattactgt
     9721 acctctgtag caatctggtt ttatgcttta atataattcc tgttggcgat aaggaaaaac
     9781 agttatttta aagagatcat agtcccgact ccgtcacgcg tgaaaagctc taaaataaga
     9841 ttatgttcaa tatttccatt aataatatgt gcaaaattta cgccattttc cattgcctta
     9901 agaagtgatc ctacttttgg aatcatacct tccccaatcg ccccggattt ttgcattgca
     9961 ttcgcttcag taatggaaag ctttctgtac accattgtcc ttttggcatc cattacgcca
    10021 tccacatcgg tcatattgat gagcttgtat gctttgagcg ctatcgcaat atctccagct
    10081 gccgtgtctg cattgatatt taaacttcca ccgtgccggt ctattgcaat cggagctacc
    10141 acaggaatgt agctcttatc aagaagcgta ttcaggagtg ccggatcgat ccattctatc
    10201 tctcccacat gccccagatc aacgtccttt tccacacccc cgacttccac ccgctggata
    10261 tccatttttt ttgcaataat caggttcccg tcacttcctg agaggccgac cccacgggca
    10321 ccgcatttgg caataagaga gacgattccg gcatttatct ttccaacaag gaccatctgg
    10381 gcgatctcaa gagtttcctg gtcggttacc cgcagccccc cgacaaacac agattctttg
    10441 cccaatgcct tcatcttttc agagatttca ggccctccgc catgaaccag aacaacacgt
    10501 attcccacgt agtgaaggag aaccgcatcc cggatagcat tttcgagaac aaccgggtcc
    10561 accatcgcat gacccccgag cttaataacg atggtcttgc catgaaactc ctgtatatag
    10621 ggcagtgcct ccataagaac gtcttcgcgt ttcatcatgt ggtatacctc ccgttgattt
    10681 ctacataccg ttcggtgaga tcacaacccc aggctgtcgc ttcttctttt ccggttgcca
    10741 ggtcaaggat gaagatcact ttttttccgt gcattgcttc ttttgccctc ttcaggtcgg
    10801 cgatgatctc acctgacttg accagtgggt attgctggtt tccatcactt atccagaggg
    10861 agactgcatt tggatcgaac ccgatgccgg cccgccctgc agcagcaacg actcttcccc
    10921 agtttggatc ttctccgtaa accgcagttt ttaccagtgg ggactcgata atggtgcgtg
    10981 caactctcgc agccgactct tctttctgcg ctcccttgac ggtgacttct attaacttag
    11041 tggccccttc accatctgcg gctatctgtt gggcaagtga acggcaacat tcctccagag
    11101 cctgagaaaa ctcagccggt ttcaccctgc cggattcacc ggttgcagta catagcgcaa
    11161 tatcattcgt gctcatatcc ccgtcaacaa caacccggtt gaatgtccgt ctggtagctc
    11221 gtttgaggga cactgaaagt gtttttgcat cgatttccac atcagtatac ataaacgaaa
    11281 gcattgtccc catctttggt gcgatcatcc cgctgccttt gccaattccc ccaatacaaa
    11341 acgattcttt ttgtacgagc gcatgtttgg gaaatgtatc agtagtcatg atggcctcag
    11401 ctgcacgttt ttcggtatca ggggtctggt cgattttttt tgccacttcc atgcactggc
    11461 gctgaatgag agggagatca aggtatcttc cgataacacc ggtactggcg acacccactt
    11521 cttcgggatc aagaccaagt gcagaaccgg caaacccggt catttttaca gcatcgccgt
    11581 accctctttt tccggtatag gcattggcac acccgctgtt cgctattact gcttccattg
    11641 tccctttttt tatctggtta cgcatcagtt ctatcggagc tgctttgaca aggttttgag
    11701 taaatacacc ggctgctgtt ccggtcgcac ggatgatggc cagcccgaac ttgccctctt
    11761 tcatcccata tgtcttcaca cccttaacgg cacatatact ccggaacgtc atttacgttt
    11821 cagctccctt tggaacagaa tgggacgatc ccactgcatt aattatatcg gcccgggcca
    11881 ggattcccac gagtttttta ccccgcatca ccgggagtcg ggaaattcct tccttgagca
    11941 tcagcgctgc agccgccccg atttccattc cctcagttgc ggtaactacc cggtgagtca
    12001 taactttttt tgcctgcata tcaccgatat ttcgaagagc atgttttgtt ttctcccaat
    12061 tgatatactc acggataggg atttcgataa tctcaaaggg ggacggtaac cagaggtcat
    12121 ccgatatccg ttcagattcc agaagagcta tgagatcgga ttctgtgagc atccccacaa
    12181 gttcagtacc ctccataacc gggagccccc cgatatgata tttcttcatt aaaccgactg
    12241 catcacggag agaagtggtg gcagtgcata caaccgggtc ttttgtcatg gcatctttta
    12301 ctaacattat tttcctccta tggaagcatc ccggccccga cgaggccatc cttttcatta
    12361 agtccgcaca tcaggttcat attctgtatt gcctgaccac tcgccccttt aacaaggttg
    12421 tcaattgcag atattgctac tacgcgcaaa ccttcacttt ccaccataag atcacagaaa
    12481 ttgctccccc gcacagcggc aagggagggt ttctgagatc gtacaaagta ttcgttttta
    12541 tagaagtcgc ggtatagttt ctcaacttct ttttgttcca tgggttcaga aagcaggatg
    12601 tgcgcagttg tgagaatccc ccgattaacc ggtacaagat ggggggtgaa atagcatttt
    12661 gccttcgagc caagccaagc gatctcctgc ttcatttccg cgagatggcg atgggtagtc
    12721 catttgtaag gtccgatgtt gtcccccacg ttcggatagt gcgttgttgc agaaggattg
    12781 tcacccgctc cactgacccc cgtcttagag tcaaagatta tggtatgagc gtgttttgcc
    12841 agtggggcgg ctgcgagtgt tgctccagtc ggaaaacacc ctggatttgc aacaaatttc
    12901 gcattgacgc attctgtgcg gtgcagctca gggattccgt agggtgcctc gaaataatcg
    12961 gtatgcataa caccgtacac tttttcatat atttctttgg gtaaacggta atcggcactg
    13021 agatccacaa ccttaattcc tctcgacaat agttttccgg catacgtcat ggcagcagtg
    13081 tgaggcaccg caagaaaggc aacatccgca tcaatggcat cgatatccgg gttttcaaac
    13141 aggagatcgg taaacccttt gaggtgggga tgaatctggt caaggggggt tcccgcgagt
    13201 ttccgggaag tagcacaggt gacctttgca gtagaatggt gaacgaggag gcgtaccagc
    13261 tcaccaccgg cataaccgga agccccgata attgcaattt tcataagatt atatccggtt
    13321 ataaaaattt aatgcttttt aagagggtat gtttaagttt tgaaaaaaat acgggggatt
    13381 ggagggaaaa aaaccttaca tttgccccag tttgcgcaaa tacagggtat gtaatcgttg
    13441 tccgagcagg tctatccgct gcttatcctc aagcccttct agtaaatctg caggaagtgc
    13501 atcaagtcct ttggcagcac caaaatatgc gccacagata agaccaatgg tatcagtatt
    13561 tccccccgca ttggctgcta cagttaagag atcagacgga tttgtatatc ttttaatgag
    13621 aaaaattgaa atggggaggg tctgaaaaac agacacatcg tttccaattt taagcagggc
    13681 cgtttctgtg tccatacctt ctttttccat tgcaagcgct tttttaattt ttccaccaag
    13741 cacttcatct tcaaattgtg ctttctccag ggccttttca acaggatccg gagaatcatg
    13801 aagcgtatga tagatgagtg tcacaaaagt tgatactgcc gcatgagctg ccggatgggt
    13861 atgggtaaca ttgcatgcac gaacagcccg ttcacatcct tcattcatat caggatatac
    13921 cagcccgaat gggatcgcta ccggaataca ccctgaagtt gttgatttta cccctttctg
    13981 aggaatattt ttacttatcg ggtgttcaca taccgctgaa acggaaccgt caggaaaacg
    14041 gagtttcccc tgcgtataga gctcacgaag cgcgacggaa tatcgttcat cattgtactt
    14101 tccgtcagca atgagagtag cgacaagaat catgacctgg gtgtcatcgg tatattgtcc
    14161 gggatttaac ccttcgttag gatggttttt gtaaggacga cggtatccgt acttcatttt
    14221 tttgagattt ggagatgtgc tttcgttggg cattcccagt gcatcaccaa ttgctgcacc
    14281 aagcaggcac cctttgaact ttttaagcat ctatcctata ttgaaaaacc cgaatagata
    14341 atccttgtta taaccctgcc gggggtatga ttattctcat ccagggttgt tgcacgtcga
    14401 tcagaatttt taaataagtt actaacatga ttaataatgt taattaggtg ggaaatgacc
    14461 gaggatttta atgtcagact cgtcgaggat gtgaaatatt acacgcccga cccacagtat
    14521 aaaaaaaatg catggatggg agactaccag aaaacctaca aaaaatttct ggctaacccg
    14581 gacgcattct gggaaaaaat tgcttcagaa cttgagtgga tccggccctg ggatgcggtg
    14641 aaggagtgga actacccata tgcgaagtgg ttcctcaatg caaaactgaa catcaccgcc
    14701 aactgtcttg accgccatgt caacaaccag cggcgcaaca aggcagcact catctggagg
    14761 ggggaggcgg gtgctgaacg catttttacc tatcagcagc tgctcagcaa agtgatgcgg
    14821 tttgcaaacg cactcaaaaa aattggtgtt aacaaaggag accgtgtctg catctacatg
    14881 ccgttggtac cggaacagat cattgcaatg cttgcctgtg cccgtatcgg tgcggtgcac
    14941 agcgtagttt ttgctggttt tggtcccgcg gccctgaaca tgcgtatcca ggatgcatct
    15001 gccaaggttg tcataactgc tgacagcagt gttcggcgcg gcaaaaccat acctttgaaa
    15061 tcaattgtgg aggaggcact tatcaatgcc ccatcagtcg aacatgtcat cgtgctcagg
    15121 cggcaggatc ccccggtcga tttaaggccc ggtctcgaac ttgatttcaa tgaaatcatg
    15181 aatagtgcat cccaggaatg tccggctgaa gttatggatg cagaagaccc gctttttacc
    15241 ctttacacca gcggatccac gggcaagccc aaagggattg ttcacacatg tgccggatat
    15301 atggttggaa catattacac cagcaaatat atcttcgata tgaaagaccc cgatattttc
    15361 tggtgcacgg cggatcctgg ctggatcacc ggccacagtt atattgtata cggccccctt
    15421 gccgtcggag caacggtgtt tatttcttag cttacgccgg attatcccga tgcaggaagc
    15481 tactggagcc tgatcgacga gcagaagatc accatttttt ataccgcacc aactgcaatc
    15541 cggatgttca tgaagatggg tgagaagtgg ccggataaat acaatttaag ttccctgcgg
    15601 ataataggat ccgtcggaga accgctcaac cccgatgctt tcgagtggta ctaccatgtt
    15661 atcgggaagg acaaggtacc gattctcgac acatggtggc agacagaaac cggtatgcac
    15721 atgatcacca cgatgattgg ggaaccaatg cgcccgggct ttgccggaaa acctattccc
    15781 ggtgtggatg cagatgtggt tgataaagac ggaaaatcgg tcaaaccggg aaccggggga
    15841 cttcttgtca tcaaatcgcc ctggccatcc atgctcagga tggtct
//
LOCUS       DASRLV010000205         6620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1134616,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000205 DASRLV010000000
VERSION     DASRLV010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 6620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6620
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134616"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1921
                     /locus_tag="VFC43_07885"
     CDS             <1..1921
                     /locus_tag="VFC43_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HZK31131.1"
                     /translation="KGGQVVQLLLFLILGPLITAFFMLLVRKEPERNWIVKLSALGIG
                     VVTVILLLSLFDKGTLYYSFAAESTSLLMFFIEMAIAVFILYLGIKYKNYLVTGLILL
                     QSGLMLYFELVYAHSVDVKYTLFIDQFSIIMALIIGIIGSLICVYALGYMKDFHAHHK
                     ELPDRRNIFFFIMFAFLSAMFGLVFSNNLLWVYFFWEITTLCSFLLIGYTKTEEATSN
                     AFTALWMNLLGGVGFAIAILYLATQTGGIMGLEALLASGKALVLIPVVLIGFAGLTKA
                     AQLPFSSWLVGAMVAPTPVSALLHSSTMVKAGVYILVRLAPIFQATFSGYILAFIGAL
                     TFLLASAIAISQSNAKRVLAYSTIANLGLIVACAGIGTYEAIWAAILLIIFHAISKSL
                     LFLSVGTVEHQIESRDIEDMNGLIMRMPKVAAMMVIGIAGMFLAPFGMLISKWAAIQA
                     FIDAPFGFIFVIILAFGSALTLFFWAKWMGRLIVVTLERENLETKIDSNIWIALVSLA
                     GLTAITCFIFPVISAMLIEPFLLQTYGNVTRLSQDNIIIMVMMLFLIVLLPVSILLPQ
                     RKYRHLTPYMGGMTTNTDLSYNGAIGLKRDLKLSNYYIIPWFGEVKLQTLGIYLCTGL
                     IGIMFVTAAYVGVIV"
     gene            1918..2775
                     /locus_tag="VFC43_07890"
     CDS             1918..2775
                     /locus_tag="VFC43_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HZK31132.1"
                     /translation="MISVAGVVVFILLSPVIGGLIAGIDRKITAHMQGRVGPTILQPF
                     YDVGKLFEKEMTVANDTQRFYVISYFIFMIFTGALFFAGGDILLVIFALTLAQIFLVL
                     GAYSANSPYSHVGAERELIQIMAYEPMVILAAVGMYVVTKSFNVSAIASYNQPLVLFL
                     PGIFAGFIYVLTIKLRKSPFDISTSHHAHQEIVKGVTTEFSGPTLGLIEITHWYETVF
                     LLGFVYLFFAFNPLIGIVAVILVYLLELLIDNASARVKWQLVMKSSWMVAAIVGVVNL
                     AILYYMGVG"
     gene            2779..3258
                     /locus_tag="VFC43_07895"
     CDS             2779..3258
                     /locus_tag="VFC43_07895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HZK31133.1"
                     /translation="MTYLSKSPWILHYDATSCNGCDIEVLASLTPMYDVERFGIINTG
                     NPKHADIFVITGSVNEQNREVVRNLYEQIPNPKVVVAVGICACSGGVFRECYNVSGGI
                     DKIIPVDVYVPGCAARPESIIDGIVTALGILEGKRNSPRSGVKEEPPAEIQQGGEAQ"
     gene            3255..3635
                     /locus_tag="VFC43_07900"
     CDS             3255..3635
                     /locus_tag="VFC43_07900"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HZK31134.1"
                     /translation="MTIEEQTIIPIEVNELVSRVEKMKKEGNRLVQIGCTGVEEGYEI
                     NYSFDKDYKFQNLRILVKQDTIVPSISGVYWGAFIYENEIHDLFGITVSGMNIDFKGT
                     LYKTTVKYPFSIPTFKEDNPCQNK"
     gene            3620..4699
                     /locus_tag="VFC43_07905"
     CDS             3620..4699
                     /locus_tag="VFC43_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HZK31135.1"
                     /translation="MSKQIIIPFGPQHPVLPEPIHLDLVLEDETVVDVLPSIGYVHRG
                     LEKLVEKREFTEYTFIAERICGICSFIHSLAYVQGVEQIMGIDVPDRAHYLRTIWSEY
                     SRLHSHLLWLGCFADSMGFENLFMNSWRIREHILDELEATTGGRVIQSACKVGGVRKD
                     ISGEQLEGMLFRLNDLKKDIHNITSIFLEDSSVKHRLKKVGILSKQDAYDCGAVGPTA
                     RGSGVVFDMRQTGYAAYGKLNFKPVVATDGDCYARCDVRIQELFSSIDIIEQAAKKIP
                     DGPIDIKITGAPNGEYFSRVEQPRGELVHYVKGNGKKNLVRSRVRTPTFTNIPPLVMM
                     LKGCQLADVPVIVLSIDPCISCTER"
     gene            4706..5107
                     /locus_tag="VFC43_07910"
     CDS             4706..5107
                     /locus_tag="VFC43_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZK31136.1"
                     /translation="MAILKMAATAIKSLFSRPATLMYPARPAKRTPATRGHIVIDPAN
                     CITCRSCQRKCPTGAICVEVKEKTWQIDRMRCVICNSCGDVCPTKCLSMDPAYPAALT
                     ARGGVYKVTVAGPKKKEPAPPATDSPKVDKI"
     gene            complement(5118..6515)
                     /locus_tag="VFC43_07915"
     CDS             complement(5118..6515)
                     /locus_tag="VFC43_07915"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase II small subunit"
                     /protein_id="HZK31137.1"
                     /translation="MLDAQQITLRFLETKLQVHPDVVRYIQEQNELELIDRIIAQVPG
                     ETIVVSAKHIPGMNATRDGTRFLSDPTVEIISGSPGTSGSVNGTSDYLHYFRDRYNRL
                     GNMIRSRCGAMPIEALTRNTRYRQEECTIIGMVVEVKTTTNGHRIAELEDSSANIPVL
                     FRKDRPVFSDAERIIHDEVIGVKGKLSNDGKLFFAEQIYRPDIRIDNTPYKSEQPGKA
                     VFISDVHVGSNTFLEDCWNRFADWLSDSDYSYLLIAGDLVDGIGIYPGQENELVIKNI
                     YEQYDAFGSMMQDLPSRMKIIISPGNHDVVRGAEPQPVIPQQFTKKFPGSCMVVENPA
                     LVNLQGVRVLMYHGRSIDDMIGLIPGASYEQSGIMMEEMLQRRHLAPAYGRRTPIAAG
                     KTDRLIIDPLPEILHTGHVHIRGLTQYRGVLGINAGTWQSQTAFQKQMNVNPTPAQAV
                     VVDLQTLIPESFTFL"
BASE COUNT         1635 a         1586 c         1623 g         1776 t
ORIGIN      
        1 aaaaggagga caggtagtgc aacttttatt atttctcatt ttaggaccgc tcatcaccgc
       61 gttctttatg cttcttgtac gtaaagaacc cgaacgcaac tggattgtaa aactatccgc
      121 actgggcatt ggtgtggtta ccgttatact tcttttgagc ttgtttgaca aaggaacact
      181 atactactca tttgcggccg aatcaaccag ccttcttatg ttttttatcg agatggcgat
      241 tgccgtattt atcctgtatc ttggtataaa atacaagaac tatcttgtaa caggtctgat
      301 ccttctgcaa tccggcctga tgctttattt tgagctggtg tacgcccatt ctgtcgatgt
      361 gaagtatacc ctcttcatcg accagttctc gatcatcatg gctcttatta tcggcatcat
      421 cgggagcctt atctgtgtat atgccctggg atacatgaaa gatttccatg cccaccacaa
      481 ggaactgccg gacaggcgta atatattctt ctttatcatg tttgcgttcc tctcggcgat
      541 gttcggtctg gttttttcca acaaccttct ctgggtatat ttcttctggg agatcacaac
      601 tctctgttct ttcctcctga taggatacac aaaaaccgag gaggctacca gcaacgcctt
      661 taccgcgtta tggatgaacc tgcttggcgg tgttggattt gcgattgcaa tcctgtatct
      721 cgcaacccag actggtggca tcatgggact tgaagcgctt ctcgcttcag gcaaagccct
      781 cgtacttata ccggtagtcc ttatcgggtt tgccggtctg acaaaagctg cacaactgcc
      841 tttttcctca tggcttgtcg gggcaatggt agcaccgaca ccggtttcgg cactgctgca
      901 ttccagcacg atggtaaaag ccggggtcta tattctcgta cgccttgccc ccattttcca
      961 ggcaacgttc tccggctaca ttctcgcttt tattggcgca ctgactttcc ttcttgcatc
     1021 tgcgattgcc atttcccaga gtaatgccaa acgggtgctt gcctattcaa ccattgcaaa
     1081 tctcggtctt atcgttgcct gtgccggtat cggaacatat gaagcgatat gggccgcgat
     1141 cctcctgatc atattccatg caatctccaa atcactcctg ttcctttcag tagggaccgt
     1201 tgaacaccag atcgaaagcc gcgacatcga ggatatgaac gggttaatca tgcggatgcc
     1261 gaaggttgca gcgatgatgg tcatcggcat tgccggaatg ttccttgcac cgtttggtat
     1321 gctgatatcg aaatgggctg cgatccaggc atttatcgat gcaccgttcg gttttatctt
     1381 cgtcattatc cttgcattcg ggagtgcact taccctcttt ttctgggcaa aatggatggg
     1441 acgacttatc gtcgttactc tggaacggga gaaccttgaa acaaaaatcg attcaaacat
     1501 ctggatcgcg ctggtatcac ttgccggact gaccgcaata acctgcttca tcttcccggt
     1561 gatttcggcc atgcttatcg aaccgttcct gctgcagacc tacgggaatg tcacacggct
     1621 ttcacaggac aatatcatca tcatggttat gatgctcttc ctgatcgtgc tcctcccggt
     1681 aagcatcctc ctgcctcaga ggaaataccg ccatttgact ccttatatgg ggggtatgac
     1741 caccaatact gatctttcat ataacggagc catcggcctg aaacgggatt tgaaactctc
     1801 aaattattat attattccct ggtttgggga ggttaaactc cagacgcttg gcatttacct
     1861 ctgtacaggc cttatcggca tcatgtttgt gactgccgcg tacgtggggg tgatcgtatg
     1921 atttctgttg caggtgttgt tgtcttcatt cttctctccc cggtgatcgg cggacttatt
     1981 gcaggtattg accggaagat aactgcccac atgcaagggc gggtcggccc cacgatcctc
     2041 cagcctttct acgatgtcgg caaactcttt gaaaaagaga tgactgtagc aaacgataca
     2101 cagcgtttct atgtgatcag ttactttatt ttcatgatct ttaccggggc actgttcttt
     2161 gccggggggg atatcctgct ggtgatcttc gctcttaccc ttgcccagat ctttttggtt
     2221 cttggggcct actcagcaaa ttctccctat agtcacgtag gggcagaacg tgaactcatc
     2281 cagattatgg catatgagcc gatggtcatt ctggccgcgg ttggaatgta tgttgtaacc
     2341 aaaagtttca acgtcagcgc tattgccagc tataaccaac ccctggtgct tttcctgccc
     2401 gggatctttg ccggtttcat ttacgtcctg acgatcaagc tacgcaaatc accgtttgat
     2461 atctccacct cccatcacgc ccaccaggag attgttaaag gggtcacgac ggaattctcc
     2521 ggccccacac tgggacttat tgaaataacc cattggtatg aaacagtttt cctgcttgga
     2581 ttcgtgtacc tgttctttgc gttcaatcca ttgatcggca tagttgctgt tattcttgta
     2641 tatcttctcg aactgttgat cgacaatgca agtgcccgtg tcaagtggca gctcgtgatg
     2701 aaaagttcgt ggatggttgc cgcaatagtt ggtgtggtaa atcttgcaat tctctattat
     2761 atgggggtgg gttgagcaat gacatatctt tcaaagtccc catggatact acactacgac
     2821 gcgacgagct gtaacggctg tgatatcgaa gtgctagcaa gtctgacgcc gatgtatgat
     2881 gtggaacggt tcgggatcat caataccgga aatccaaagc atgcggatat ctttgtcatc
     2941 accggctcgg taaatgaaca gaaccgggaa gtggtgagga atctctatga acagataccc
     3001 aacccgaagg ttgttgtcgc ggttggtatc tgtgcctgtt ccggaggagt tttccgggaa
     3061 tgttacaatg tgtcaggtgg tattgacaaa attatcccgg tcgatgttta cgttcctggt
     3121 tgtgctgccc gtcctgagtc aattatcgat ggaattgtga cagcactggg aattcttgaa
     3181 ggaaagagga attcaccacg gtccggggta aaggaagaac cccctgcgga aatacaacaa
     3241 ggaggtgagg cacaatgaca atagaggaac agaccatcat accgattgag gtgaatgagc
     3301 tggtctcccg ggtcgaaaag atgaaaaaag agggcaaccg gcttgtccag atcgggtgca
     3361 ccggggttga agaaggctac gagatcaatt actcatttga caaggattac aaattccaga
     3421 accttcggat cctggtcaaa caggatacta ttgtgcccag catcagtggt gtctactggg
     3481 gggcatttat ctacgaaaat gagatccacg acttgttcgg gattacagtt tcaggaatga
     3541 atatcgattt caaaggcacg ctctacaaaa caacggttaa gtatccgttt tccatcccga
     3601 cgttcaagga ggacaaccca tgtcaaaaca aataatcatc ccgttcggac cccagcaccc
     3661 ggtgctccct gaaccgatcc acctcgacct tgtgctagag gatgagactg tggtcgatgt
     3721 gctcccctca atcgggtatg tgcaccgcgg cctcgaaaag ctcgtagaaa aacgggaatt
     3781 tactgaatat acctttatcg ctgaacgcat ctgtggtatc tgcagcttca tccacagcct
     3841 tgcttacgtc caaggggtcg aacagatcat gggaatagat gttccggacc gggcacacta
     3901 cctgcggaca atatggtctg aatattccag gcttcactcc catcttctct ggcttgggtg
     3961 ttttgctgat agtatgggtt ttgagaatct gttcatgaac tcctggagga tccgcgagca
     4021 tattctcgat gaactcgagg caaccacggg cggtcgggtg atccagagtg cctgtaaagt
     4081 cgggggagtc agaaaagata tctccggtga acaactggaa ggaatgcttt tccggctgaa
     4141 tgatctgaaa aaagacatcc acaacattac cagcatattt ttggaagaca gctcggtgaa
     4201 acacaggctg aaaaaagtag gtatactctc aaaacaggat gcttatgatt gcggtgcagt
     4261 gggacctact gcccggggaa gcggagttgt gtttgacatg cggcaaacgg gatatgccgc
     4321 atatggcaaa ctcaacttca aaccagttgt cgccacggat ggcgactgct atgcacgctg
     4381 tgatgtgaga atacaggaac tgttttcttc aattgatatc atagagcagg cggcaaaaaa
     4441 gataccggat ggcccgatcg atattaaaat taccggcgca ccgaacggag aatacttctc
     4501 ccgggttgag caaccccggg gtgagcttgt ccattatgtc aaagggaacg gaaaaaagaa
     4561 cctcgtccgg tcgagagtga ggacacccac atttaccaat attccacctc tggttatgat
     4621 gttaaaaggg tgccagcttg cggacgttcc ggttatagta ttgtcgattg atccctgcat
     4681 cagctgtacg gagaggtgac aaacgatggc aattcttaaa atggcagcaa cggcgataaa
     4741 aagtctgttt tcacgcccgg caacgctgat gtatcctgcg aggccggcaa aacggactcc
     4801 ggcgacccgg gggcatatcg taattgatcc cgcaaactgc atcacctgcc gttcatgcca
     4861 gcggaagtgt ccgacaggtg caatctgcgt ggaagttaaa gagaagacat ggcagattga
     4921 ccggatgcgg tgcgtgatct gcaattcctg tggggatgta tgtcccacca aatgcctcag
     4981 catggatcct gcctacccgg cagccctcac tgcacgtggc ggggtttaca aagttaccgt
     5041 tgccgggcca aaaaagaaag aaccggcacc acctgcgacg gacagcccaa aggtggataa
     5101 gatttaaaaa aatattttta taaaaaggta aaagattccg gtatcagggt ctgaagatca
     5161 acgacaacag cctgggccgg ggttgggttc acgttcatct gtttctggaa tgcggtctgg
     5221 gactgccatg taccggcatt aatcccaagc acaccccgat actgtgtcag tccccggatg
     5281 tggacatgac cggtgtgaag gatttcaggg agcggatcga ttatcaacct gtcagtttta
     5341 cctgcagcga tgggtgtcct gcgcccataa gccggagcaa gatgccggcg ctggagcatc
     5401 tcttccatca ttatcccgct ctgctcatag gacgctcccg ggatcagacc aatcatatca
     5461 tcgattgatc tcccgtggta catgagaacc ctgactccct gaaggttgac aagtgccgga
     5521 ttttcaacaa ccatgcagct gcccgggaat tttttggtaa actgctgggg tattaccggt
     5581 tgtggctctg cccccctgac tacatcgtga ttaccgggtg aaatgatgat cttcattcgt
     5641 gaaggaaggt cctgcatcat tgagccgaac gcgtcgtatt gttcgtagat attttttata
     5701 acgagttcgt tttcctggcc cggatagata ccgataccat cgacaagatc tccggcaatc
     5761 aggagatacg agtaatccga atccgaaagc cagtcagcaa acctgttcca gcaatcttcg
     5821 agaaatgtat tgctgccaac atggacatcg gatataaaaa ccgctttgcc tggctgttcg
     5881 cttttgtagg gcgtattatc gatgcgaata tcaggtcggt agatttgttc ggcaaaaaag
     5941 agtttcccgt cattggagag tttgccttta accccgatta cttcgtcgtg gataattctt
     6001 tctgcatcgg agaagacagg gcggtcttta cggaatagta caggaatatt tgcagaagaa
     6061 tcttctaact ccgcaatacg gtgaccattt gtggtagtct tcacctccac aaccatacca
     6121 ataatagtac attcttcctg acggtaacgg gtattccggg tgagtgcttc tatcggcatc
     6181 gccccgcatc ggctcctgat catgtttccc agacggttat aacggtcacg gaaatagtgc
     6241 agataatcac tagttccgtt cacactaccg gatgttcccg ggcttccact gattatttca
     6301 acggtaggat cagagagaaa tcgcgtcccg tcccgggtgg cgttcatgcc aggaatatgt
     6361 ttagcagaaa caacgatggt ctccccggga acctgcgcta taatgcgatc aatgagttcc
     6421 agttcattct gttcctggat ataacggaca acatcgggat gcacctgcag cttggtttca
     6481 agaaaccgga gggttatctg ctgtgcatca agcatgaata gtatatgcgc atggagaaaa
     6541 tatagggttt tattatggca ccatgaaaat tacaaaaaat cagataggta ctatgatgaa
     6601 ggattgatat tcttttcagg
//
LOCUS       DASRLV010000206         5715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_565648,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000206 DASRLV010000000
VERSION     DASRLV010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5715
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565648"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VFC43_07920"
     CDS             complement(<1..445)
                     /locus_tag="VFC43_07920"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HZK31138.1"
                     /translation="MQGLNRENILVRVFQHTSSEPLGYLESIFSGEGITFEYVRLWED
                     DPVNMGSATHFVFLGGPMSVNDEVQLPWLNEEKELIRRAAKKGSPVLGLCLGAQLIAS
                     AHGAPVYQYINETGWYPVHPVKDASGVLATFPDPFYVFQMHGETFH"
     gene            complement(668..1573)
                     /locus_tag="VFC43_07925"
     CDS             complement(668..1573)
                     /locus_tag="VFC43_07925"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HZK31139.1"
                     /translation="MSKDYFIDKVLATTVVGSYPVVKGSGLRSIFDPLHTAVERAVAD
                     QIAAGIDIISDGQVRGDMIQVFTSKLPGVKGQDVIGKVQPASGPITAGDTKYALAQFP
                     KVKGIITGPTTLAHGLHLNTPMYRNKEELALDLAAALVPEAKALEAAGVTLLQIDEPI
                     FSTGMADLSVGKEAIGIITAALRIPTCMHVCGNLGNVIDEILKFNVNVLDFEFANNPA
                     NLDLLSRRDLAGRMIGYGCVDSSVDKVESVAEIKKRIEKGIEIFDPRIILLDPDCGMR
                     MRSRDVAFAKLKHMTEAARMVRIGL"
     gene            complement(1634..2926)
                     /gene="aspS"
                     /locus_tag="VFC43_07930"
     CDS             complement(1634..2926)
                     /gene="aspS"
                     /locus_tag="VFC43_07930"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050560 - aspartate-tRNA(Asn) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HZK31140.1"
                     /translation="MRIPIQSVTPASGQAEVFGWVHEVRDLGGLAFFLIRDRTGIIQV
                     TIPKKKASEAVLAAAKKVSRESVVRVVGTVKAIDKAPGGRELVPDILEIISLADSPLP
                     LDVVEKVPADLDTRLDARFLDARRPRVAAVFQIRSAAMHAISQYLHDEKFINITTPKI
                     VAAATEGGTELFPIAYFDKEAFLNQSPQLYKQMMMAAGFEKVCEIGPIFRAEEHNTTK
                     HLNEATSIDIEVSYADHLEVMCILEEMMVKTYEYVSTSCGDQLSNLEISDFVVPKAPF
                     PRLPYGEAISIAQKKIEDPIKYGDDISPAAERAIGAEMGGHYFIVDWPSEIRPYYAMP
                     YENDPSVCKAFDLMHPRMELSSGAQRVHQHDLLVEQIKKKGLNPESFEFYLRPFRFGM
                     PPHAGWGLGADRLIMTMLGLSNVREAVLFPRDMHRLVP"
     gene            complement(3193..5190)
                     /locus_tag="VFC43_07935"
     CDS             complement(3193..5190)
                     /locus_tag="VFC43_07935"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HZK31141.1"
                     /translation="MSHRKLTLIALTSIVLILAAVIACGCMSTTVPVPAGEIKKFSSA
                     DEIRDYIRNTTNLAQTTYYRGDGVIAPVPSPVFVGQESSAKGVSSSDSLNYAGGLGTT
                     GYSQTNVQVVGVDEPDFVKNDAKYIYLISGSMLTIVDAYPPVSASVISRTELEDTPRE
                     IFINGDRLVLLKTGSSDIAVSSSPPASDSSEKVAIMPPRYPYYRSAPVTHAVFYDISD
                     RAHPKVVKDYTIDGDYVNARMIDNIVYLLSRESVYPYDDRIIVPAVRESTKVILAPDV
                     YYFDNPEPQYDFTTITSFDVGNGITKDAKTFLVGSGNIIYVSPQALYVTYQKYQNIYQ
                     PMRGVAEPVMGVVQGSGESSSGIARVSSSSPITDFNTMSEAEKQALIADMKSGEQEAI
                     QKREIDQTITMIHKIGINNGAITYLAKGEVAGYLKNQFAMDEFGGNLRAATTSDVWTN
                     RGQYEYNNVFVLDGAMKTIGSLTHIAEQEKIYSTRFIGDRLYMVTFKRIDPFFVIDLS
                     TPASPKILGKLKIPGYSDYLHPYDKNHIIGIGKETATTEWGGVSTKGLKIALFDVTDV
                     EHPQQISKVEIGDSGTDSAVLTDHRAFLFDKNKNLLVVPARVVQSLDIPMKVSGDQQR
                     IWYGAYVFGITPETGFVLKGTVEHNTGGGYAWYGSPYEVRR"
     gene            complement(5295..>5715)
                     /locus_tag="VFC43_07940"
     CDS             complement(5295..>5715)
                     /locus_tag="VFC43_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31142.1"
                     /translation="QTNYHYDFAVHVKSLNSITSVARRKKDDFAMVFTSGITSYSNDT
                     DSGPTEKFIQNYGTRVHHMAFRTTEIETTVRFLKNDGMGFLLEVVGSEEEGLKQIFSE
                     PSQNTLIVNEYIIRYGDFDGFFTKSNVERLTKATEKQ"
BASE COUNT         1396 a         1394 c         1382 g         1543 t
ORIGIN      
        1 ggtggaaggt ctcaccatgc atctggaata cgtaaaaagg atccgggaag gtagcaagga
       61 caccgctcgc atcttttaca gggtgcaccg ggtaccaccc tgtttcattg atatactgat
      121 ataccggggc tccgtgtgcc gatgctatca gctgggcacc aaggcacagg ccgagtacgg
      181 gacttccctt ttttgctgcc ctccggatta gttctttctc ctcattgagc caaggtagct
      241 gcacttcatc gttcacgctc atcggcccgc cgaggaaaac aaagtgagta gctgatccca
      301 tattaacagg gtcatcttcc cacagccgta catattcaaa cgttatcccc tctcctgaaa
      361 agatactctc gaggtaaccg agcggctctg aggatgtatg ctggaaaacc ctgaccagaa
      421 tattctctct gtttaatccc tgcatagaat ttccggttca atatttctgg cgtaacacat
      481 ttcggttttt tggtcgtggt tcaccaggca acgtttgtca tactgcaata tcactactca
      541 atattggtac aaaaaacggt tattgcgatc gatgttttta ttaaggagaa tttgctgttc
      601 gactgcgatc ctcatgcatg atcggtctta cgtataggat ctcataaaaa agcccgaatc
      661 agaagaatca aagaccaatg cggaccatcc tggccgcttc agtcatatgc ttcagtttag
      721 caaatgccac atcacgggat cgcatccgca tcccgcaatc cggatcgagc agtataatcc
      781 gcgggtcaaa aatctcaata cctttttcaa tccgcttttt tatctctgca acgctctcga
      841 ccttatcaac cgacgagtcc acacagccat agccgatcat ccggcccgcc aggtctcgcc
      901 gggatagcag gtcgagattt gccggattat tcgcaaactc gaagtcgagt acattgacat
      961 tgaatttcag gatctcatcg atgacattcc ccagatttcc acagacatgc atgcaggtgg
     1021 gaattctgag agctgcagtg atgatgccaa tagcctcctt tcccacagaa agatctgcca
     1081 ttcccgttga aaatatcggt tcgtcaatct gcaacagcgt tactcctgcg gcctccagtg
     1141 cctttgcctc aggtaccaga gcagcggcaa gatccagggc cagttcctcc ttgttccgat
     1201 acattggtgt atttaaatga agaccatggg caagtgttgt cggacccgtg ataatccctt
     1261 tcactttcgg gaattgtgcg agagcatact ttgtatctcc ggctgttatg ggcccggatg
     1321 caggctgcac tttaccgata acgtcctgcc cttttactcc gggtaatttt gaggtgaata
     1381 cctgtatcat gtcacccctt acctgcccat ccgatatgat atcaatacct gcagctatct
     1441 gatcggcaac tgctctctcg actgcggtat gaagaggatc aaagatactc ctcagcccac
     1501 tcccttttac cacagggtaa cttcctacaa ccgtggttgc aaggactttg tcgatgaaat
     1561 aatcctttga catagggtat ccggatattt tatgtacaat cgaattgacc gcggcgataa
     1621 aaaatgtatg tggtcaggga accagccggt gcatgtcacg agggaaaagt actgcttccc
     1681 gtacatttga tagtccgagc atcgtcataa tcagccgatc ggctcccagt ccccagcccg
     1741 cgtggggtgg cattccaaac cggaacgggc gcaggtaaaa ctcaaaactc tcaggattca
     1801 gccctttctt ttttatctgc tcaacgagga ggtcgtgctg gtgcacacgc tgtgctccgc
     1861 ttgataactc cattctcggg tgcataaggt caaacgcttt gcagacagag ggatcattct
     1921 catagggcat ggcataataa ggacgtatct cgctgggcca gtccacgatg aaataatgtc
     1981 cacccatttc tgctccaatg gctctctctg ccgcaggact gatatcatcc ccgtatttga
     2041 tgggatcttc gatctttttc tgggcgatac tgatagcctc accataagga agccggggaa
     2101 aaggggcttt tgggacgaca aaatcggaga tttcgaggtt tgaaagctgg tctccacagc
     2161 ttgtgctgac atattcgtag gtttttacca tcatctcttc aaggatgcac attacttcga
     2221 gatggtctgc atatgagacc tcgatatcaa tcgaggtggc ttcattcaga tgtttggttg
     2281 tattatgttc ctcagcgcgg aatatggggc cgatctcaca gactttttca aatcctgcag
     2341 ccatcatcat ctgtttatag agctgcggac tctggttgag aaatgcctct ttgtcaaaat
     2401 aggcaatcgg gaatagttcg gttccgcctt ccgttgcggc tgcaactatc ttgggggtgg
     2461 tgatattgat aaatttctcg tcatgcaggt actggctgat ggcatgcatc gcagcgctgc
     2521 gaatctgaaa aacagcggct acccgtggtc tccgggcatc aaggaaccgt gcatcgaggc
     2581 gggtgtcgag gtcagccggg accttctcca ctacatcaag gggaagtggg ctgtccgcaa
     2641 gactgatgat ctcgagaata tccggcacaa gctcgcgtcc accgggtgct ttatctatag
     2701 cctttacggt ccccacgacc cggacaacag attcgcggga gacttttttt gctgcggcaa
     2761 gaaccgcttc agaagctttc ttcttgggaa ttgttacctg gatgattccg gttcggtcgc
     2821 ggattaaaaa gaaggcaaga cccccaagat cccgcacctc gtggacccat ccgaaaactt
     2881 ctgcctgacc acttgcaggt gtaactgatt gtataggaat gcgcatgatg aaatcctata
     2941 tctatgtggt gcaggggatt atggatttta tgggaataat cccaccattt caacagtccg
     3001 ggaaatcgtt attgtatatt ataagaaaaa ttacggcaca aaaagggtat ttcagattcc
     3061 tttcagttcc gggtagagca catctcctcc ccccggcagc ttgatcgtgg tgaccgttgt
     3121 atttattttg cttaaggaat tgacaaggat ctgtttttgg gagagggtat aaagagtatc
     3181 accgatgtag agttagcgtc tgacttcgta cggcgaaccg taccaggcat agcccccgcc
     3241 agtgttgtgc tcaacagtcc ccttcaggac aaagccggtc tctggtgtga taccgaacac
     3301 gtatgcgccg taccagattc gctgctggtc gccactcact ttcatgggta tatccaatga
     3361 ctggactacc cgggccggaa ctacgaggag gtttttattc ttatcaaaga ggaatgcccg
     3421 gtgatccgtg agtacagccg agtcagtacc ggaatcacca atctccactt tagagatctg
     3481 ctgcggatgc tccacatccg ttacatcgaa cagcgcaatc ttcagacctt ttgtggagac
     3541 acccccccat tcagttgttg cggtttcctt gcctattccg atgatatggt tcttatcata
     3601 gggatgcagg taatcggaat agccggggat tttaagttta ccgaggatct ttgggctggc
     3661 aggtgtcgag aggtcgatta caaagaacgg gtcgatgcgt ttgaaggtca ccatgtaaag
     3721 gcggtcaccg atgaaccggg tggagtagat tttttcctgt tcagcaatgt gggtgagtga
     3781 cccgatggtc ttcatcgcac catccagtac aaagacgtta ttatattcgt actgcccgcg
     3841 gttcgtccag acatctgatg ttgtcgctgc acggaggttg ccaccgaact cgtccattgc
     3901 aaactggttc ttcagatatc cggctacttc accttttgcc aggtatgtga ttgcaccatt
     3961 gttgataccg atcttgtgga tcatggtgat tgtctggtcg atctcgcgtt tctgtattgc
     4021 ctcctgttca ccactcttca tgtccgcgat cagcgcctgc ttctctgctt cactcatcgt
     4081 attgaaatcg gttatcgggg aggaggatga aacacgtgca atacctgaag atgattcccc
     4141 ggaaccctgc acaacgccca taacgggttc tgccacaccc cgcatcggct gataaatgtt
     4201 ctggtatttc tggtaggtta cgtacaatgc ctgcggagag acgtaaataa tattcccgct
     4261 gccgacaagg aaagtttttg catcttttgt gatgccgtta cccacgtcaa acgaagttat
     4321 ggtcgtaaaa tcgtattgcg gttccggatt atcgaaatag tacacatccg gcgccaggat
     4381 gactttggta ctctccctca ctgcaggaac aatgatccgg tcatcatacg ggtacaccga
     4441 ctcccgggac aggaggtata cgatgttatc aatcatacgg gcgtttacgt aatcgccgtc
     4501 aattgtgtaa tctttgacaa ccttcgggtg tgcccggtca ctgatatcgt agaagactgc
     4561 atgcgtgacc ggtgcagaac gatagtatgg gtaccgcgga ggcatgatgg cgactttctc
     4621 tgaagaatca gatgctggtg gtgaactgct gactgcaata tctgaagaac ccgtcttaag
     4681 gagtacaagc cggtcaccgt tgatgaagat ctcacggggt gtatcctcaa gttcggttct
     4741 tgaaatgacg gatgcagata ccggggggta ggcatccacg atggtaagca tgctaccgga
     4801 aatcaggtag atatattttg catcgttctt tacaaaatcc ggctcatcga ctccgacaac
     4861 ctgaacattg gtctgggagt aaccggttgt tcccaagcct cccgcgtaat tgagggaatc
     4921 agaagatgaa acgcccttgg ccgatgattc ctgtccaaca aagaccggag aaggtactgg
     4981 agcgataacg ccatcaccgc gataatatgt tgtttgtgcg aggtttgtgg tattcctgat
     5041 ataatcacgg atttcatccg ccgaactgaa ctttttaatt tccccggccg gtacaggtac
     5101 tgttgtgctc atgcagccgc aggctatcac cgcagccagg atgagcacaa tcgaagtaag
     5161 tgcgatcagg gttaatttcc tgtgtgacat ggtgtatcaa tgaccctttg ggagcaaaag
     5221 ataatcaaac tggcgttaac ttcgaaaaaa actgaatcta ttgcattgtt catttggaat
     5281 aataaggcaa aatctcactg tttttctgtt gcttttgtca gccgttcaac attgcttttt
     5341 gtgaagaatc cgtcgaaatc accataacga atgatatact cgttcacgat cagggtgttt
     5401 tgtgaaggtt cagagaatat ctgtttgagc ccttcttctt cagaaccaac gacctcgagc
     5461 aggaatccca ttccgtcatt tttcaaaaat ctgactgttg tttcgatttc ggtcgtacgg
     5521 aatgccatgt gatggacgcg tgttccataa ttctgtataa acttctcggt cggaccggaa
     5581 tcagtatcgt tcgagtaaga agtaatgccg gaggtgaata ccatcgcaaa gtcgtccttt
     5641 ttcctgcggg caacactggt gattgaatta agggatttga catggaccgc aaaatcgtaa
     5701 tgataattgg tctga
//
LOCUS       DASRLV010000207        13164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1135099,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000207 DASRLV010000000
VERSION     DASRLV010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 13164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13164
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135099"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VFC43_07945"
     CDS             complement(<1..208)
                     /locus_tag="VFC43_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZK31143.1"
                     /translation="MLTVLLIDDQNPLLEVMHPLLERIGHMKVKTALSAKEALDLLVS
                     TTFDAMIVDYDLPEINGIEFLKIIR"
     gene            complement(257..745)
                     /locus_tag="VFC43_07950"
     CDS             complement(257..745)
                     /locus_tag="VFC43_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HZK31144.1"
                     /translation="MISKTMEQALNKQVNREFYSAYLYLAMSAYFEAANMKGFAKWMR
                     IQAKEEQGHALKMYDFIIARGGKPNLMDIEAPKAKWASAGKVFEEVYAHEQKVTSMIH
                     GLVELAIKEKDHASFEMLQWFVKEQVEEEEHASAILAQVMCVGDVPGHLFYLDHHLGK
                     RE"
     gene            complement(828..1430)
                     /locus_tag="VFC43_07955"
     CDS             complement(828..1430)
                     /locus_tag="VFC43_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HZK31145.1"
                     /translation="MSFFIINPEIYSWVILPLLIFIARICDVSMETIRVIYISKGIKY
                     LAPIIAFFEIVIWLLAMEMVMNDLSNIANFLAFAFGFAMGTYVGLVIEEKLSIGKVIL
                     RIVTTGESNDEIISFMRSENYGTTMIDATGSRGNVKMILSLVNRTEVAHITEKIQTTN
                     PHAFFSIEDVRYVNEEVFRPKKPNTLTGFFHSFIRPRKKK"
     gene            complement(1580..1720)
                     /locus_tag="VFC43_07960"
     CDS             complement(1580..1720)
                     /locus_tag="VFC43_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HZK31146.1"
                     /translation="MAIDPICKMTVDEKTAKFTSEYQGKKYYFCAPGCKKKFDTEPQK
                     YV"
     gene            complement(1734..4166)
                     /locus_tag="VFC43_07965"
     CDS             complement(1734..4166)
                     /locus_tag="VFC43_07965"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZK31147.1"
                     /translation="MADTEKKKAELKISGMHCATCAINIEESLGQLKDVSKVQVNFGT
                     DTAHVEFDPKKVSLTELEKAVKTAGYDVVNHEVTIKIGGMVCATCVQTIETALRALPG
                     IISANVNLGTEKAYVAFNPSISTIPEMKKAIEDAGYQYLGIAGEVSEEAEKIAREKDL
                     HDKFLRFSIGFAVSIPLMIAMWVPLPISMHTLAYIMLAVSAPVFYFVAYPIFRAASMA
                     LRNRSLNMDVMYAMGTGVAFVASVMGTFGIILTHEFMFYDTAIMLASFLMLGRYLEAR
                     AKGRTSEAIRKLAGLRAKTATIIREGREEEIPVEDVVVGDIVVVKPGSKVPVDGEVVG
                     GASYVDESMITGEPVPPLKSPGSRVVGGTINTNSVLSVKATKVGRETVLAQIIQLVED
                     AQGSKPPVQRIADIAVTYFIPAVIAIAGISFIVWFFVLNSTLLFALTALISVLVVACP
                     CALGLATPTAVTVGVGRGAELGILIKNGEALEIAEKVTTVVFDKTGTLTKGKPEVTDI
                     IPFGISESTLLGLTAGVEMNSQHPLAQAVVRKAKNAGISIPQADHFDTFGGKGVVATV
                     LSENVLAGNRLLMQEKGVTVSDAVEVQIAAFEQDGKTAILIAAGMQLAGIIAIADTLK
                     ETSGESVRQLKKMGIQVVMITGDNKRTADAISRQIGIDRVIAEVLPQDKAAEVKALQE
                     KGEIVAFVGDGINDAPALARADVGIAIGSGTDIAIESGDVVLIKDDLLDAVAAIQLSK
                     KVMGRIKGNIFWAFAYNTALIPVGAGVLYPFFGFTFKPELAALAMAMSSVTVVSLSLL
                     LKKYIPEAKQGR"
     gene            complement(4286..4564)
                     /locus_tag="VFC43_07970"
     CDS             complement(4286..4564)
                     /locus_tag="VFC43_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAC family transcription factor"
                     /protein_id="HZK31148.1"
                     /translation="MGGDDKDGYYCSICGGIPPDKITTKRVLIDGKETGIDHLDWIFS
                     EVKKLGPDNDAVLAAELLKRVKEFNYVPTKKTEVYATALVEEYRRRNS"
     gene            complement(4569..4919)
                     /locus_tag="VFC43_07975"
     CDS             complement(4569..4919)
                     /locus_tag="VFC43_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZK31149.1"
                     /translation="MDTSDKPDKKRDELKGKVLQLKDLVNYNEGTVASRMIISRKAGN
                     ITIFSFDENEGLSEHTAPFDAVVTILEGESEVWVAGKTFTMKSGDTIIFPANVPHALS
                     AITKFKMSLTMIKE"
     gene            complement(5704..7842)
                     /locus_tag="VFC43_07980"
     CDS             complement(5704..7842)
                     /locus_tag="VFC43_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HZK31150.1"
                     /translation="MTLENRKQGQSPVTDDVKYNRLIHEKSPYLLQHATNPVDWYPWG
                     AGAFLRAAHEDKPVFLSIGYATCHWCHVMAHESFEDNGIAALLNRDFICIKVDREERP
                     DIDSIYMSVCQMMTGQGGWPLTVIMTFEKKPFFAGTYFPRESRFGITGLIDLIPRITR
                     LWHEKREDLFRSADQITAALHQEQDTMPAGIPGISLLNSGYEELALRFDSENGGFGHA
                     PKFPTPHTLLFLLRFWRRTGNEHALFMVEKTLHELQSGGIFDQIGGGFHRYSTDMQWR
                     VPHFEKMLYDQALLVMAYTEAYQALKKHAFRKTAQEIITYTLRDMISPEGAFYSAEDA
                     DSGGGEGSFYLWTSQEMENILGKDDAMYATRAFNVTTSGNYHPAEGGNAQNILFLQPS
                     MTESEYSPGLLKASPELRLDSVRARLFKARSHRSRPSLDDKILTDWNGLFIAALAQAA
                     RTFKNEDYLTAARRAMEFILTHMRDSKGRLWHRYRDSEPAIPAFADDYAFIVKALIEL
                     YESTFEPSYLQAALELNAVFIEHFWDKEKGGFFTISDDGEVLLIRKKEFYDGAIPSCN
                     SVALENLIRLAHLTGETKTEERAFELLRCFMPSVQQSPSAHTWYLCDLDTIIGPLQDV
                     VIAGEQDAEDTGILIRALHDHYFPHIVVICRSPGAVSDQLNIIAPFTQNMHAMGGKAT
                     AYICSGHICASPTTESPRMLELLLESSPKK"
     gene            7881..8672
                     /locus_tag="VFC43_07985"
     CDS             7881..8672
                     /locus_tag="VFC43_07985"
                     /inference="COORDINATES: protein motif:HMM:NF023096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HZK31151.1"
                     /translation="MQSGDSGEVMCSLEPNKVGIMVLTAVPFLTVIFIGIIVILISRT
                     LDVLWAQVLPMRFFYSLLRAPGVIIHELSHVLGCLISGAKVKKVIYFSNEGGSVTYSS
                     PKIPYIGDLVINIAPLFCIPLILAGCTWIFSEYLGCVFPPMPMGVNSLDALFGVVTGI
                     LGMFTSNLIIRFNPWFLAYLYLNLTLVLSVAPSSQDIKNAAVGIGVITLMGMMILWSS
                     NTLAGNLLDGFVYLLGIGFSLGLLFGIIALVISSPLVILYVHKKI"
     gene            8738..11419
                     /gene="mutS"
                     /locus_tag="VFC43_07990"
     CDS             8738..11419
                     /gene="mutS"
                     /locus_tag="VFC43_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HZK31152.1"
                     /translation="MIHESFPDRSPGPENGVRLTPVMRQYHEIKDKYPDTILFFRIGD
                     FYETFESDAELISRELEIVLTSRSKSGDTKIPLAGVPYHSADGYIAKLVGKGYKVAIV
                     DQVGDTKNTKGIVKRELVRVITPGTVIDSAMLSSSGSSSLLAICPDRKNSHWGIALLD
                     ISTGEFFVTLVDHDKGFLNILSEIARNRPAECIVPSTITDDLKQRIRDRDVVVTCLDD
                     REFSEQRARDTLLEHFHVVSLAGYGCDDFPAAISAAGAALSYAKETQRSSLTHISSLV
                     LRSSEQGLMLDAITLRNLEIKESIRHDTKGATLLSCLDLTKTSMGSRVLAQRLSRPIT
                     GIEEINKRLDAIEFFIDNTQVRLAVRSLLSRFADIERIAARIAYGNAGPRDLLALAES
                     LESIPDLKNSFQVFRAPDVPDQIMNALNDLNDLPDSIHLIRGAIVDDPPAIARNGGVI
                     RQGFSQDLDTIKGVLHSGKGWILELEQKEREKTGIKSLKIGYNRIFGYYIDITKPNLH
                     LVPSHYERRQTTATGERYILPELKEKETLITNADERVLALERELYGGLVETLRTIIPE
                     LQAIARGTALMDVSASLAEIAKTHDYVRPQLNSGDAIIIRDGRHPVVEQGAFGDFVPN
                     DTDLSSSGTQVMIITGANMAGKSTYMRAVALICIMAQAGSFVPARHASIGILDRIFTR
                     VGAFDDLASGQSTFFVEMLELANILNNVTPRSLVILDEIGRGTSTVDGCSIAKAVLEY
                     LHGRPGAAPKTLFATHFHELVEVEGVLKRVKNYHFAVKETKNDVIFLRKLIPGATDKS
                     YGIHVARLAGIPKKVTERADSILTETMNREVPSGSRPQRYTQILLFDNENPQGPVTTH
                     PALEKLAGINPDEITPLQALAIIAELRKMLNRKGNKP"
     gene            11416..>13164
                     /gene="mutL"
                     /locus_tag="VFC43_07995"
     CDS             11416..>13164
                     /gene="mutL"
                     /locus_tag="VFC43_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HZK31153.1"
                     /translation="MRTTIRILDQATVNKIAAGEIVERPASVVKELVENAIDAGAHVI
                     RMDISSSSEGIESIRITDDGMGMSPDDAVFAFTPHATSKISGIEDLNHITTLGFRGEA
                     LASIAAVARITLITRLRGTSVSGTKVVVEGGKLLAKSDFGAPEGTSVLVEDLFYNTPA
                     RKKFQKSKNTEIGHIHALLEGICLSHPEISFRLFFNRREQLVTDRTALLLDTIARLYG
                     NDMVKDLVPVHLSLPFMKISGYTSRPSFTRKDPSRMMITINQRYVSSAIVTGAIKDGY
                     GSLLPKDLFPAAFLSLTIDTSLVDVNVHPTKKQVRLSREGEITQAICDAVKTALLSQD
                     LIPHIGQPSDVYMSEDCRNNEAGSDTHYDFSGLLPEGIRETVHAGTFLTDRQLRQTEL
                     STVITPVDVQLPAMEVIGQFGGIYILATTDAGELILIDQHAVHERILYEQVSTRTEGI
                     HRSQELIVPVLLHRTLKDASILRDIIPALAREGFILEEFGKDTFLVRAIPVVLGKLED
                     TTMIDDMVSDLVNDEAVRTVNNREKIARIIACRGAIKAGTVCSREQCQRLIEQIRYVK
                     NPFTCPHGRPTMIRFSR"
BASE COUNT         3375 a         3208 c         3007 g         3574 t
ORIGIN      
        1 ctctgataat ctttaaaaat tctataccat tgatctctgg caggtcgtaa tcaacaatca
       61 tcgcatcgaa ggtggtgctc accaggagat ccagggcttc ttttgcgctc agtgctgttt
      121 tgaccttcat atgaccgatg cgttcgagta gcggatgcat gacctcaagc agaggattct
      181 ggtcatcaat aagaagcacg gtaagcataa ggtaacctac gatggtatcg cttgaaaaaa
      241 ataaaaagat tgggatctat tcccgttttc ccaaatgatg gtcaagataa aagagatgac
      301 cgggtacatc cccgacacac attacctgtg cgagtattgc acttgcatgc tcctcttctt
      361 cgacctgttc ttttacaaac cactgtaaca tctcaaaact tgcatggtct ttttccttga
      421 ttgcaagttc gacaagtccg tggatcattg aggtcacttt ttgctcatgg gcatatactt
      481 cctcaaagac tttaccagca gaggcccatt tcgccttggg tgcttcgata tccatcagat
      541 ttggtttacc acccctcgcg atgataaaat cgtacatctt caaagcatga ccctgctctt
      601 cttttgcctg tatccgcatc catttcgcaa atcctttcat gtttgccgct tcgaaatacg
      661 cagacatcgc aaggtacagg tatgccgaat agaattcccg gttcacctgt ttgtttaagg
      721 cctgttccat agtctttgaa atcatgtaat aacctctttt cactcgatga ttggtttttc
      781 ctttattaat gctttcgtaa tgaggaaaga aggtcggtaa ttttagatca ttttttcttt
      841 cgtggacgga tgaatgaatg aaaaaaaccg gtaagtgtgt tgggtttttt aggtcgaaaa
      901 acctcctcat ttacatacct gacgtcctcg atggaaaaaa atgcgtgtgg atttgttgtc
      961 tggatctttt cagttatatg cgccacttca gtacggttga caagggagag gatcatcttg
     1021 acattgccac gggatcccgt ggcatcgatc atcgttgtac cataattttc cgaacgcatg
     1081 aaagaaatga tctcatcgtt agattccccg gtcgtcacaa tacgcaaaat aacttttcca
     1141 atagaaagct tctcctcaat cacaaggccg acatacgtac ccatggcaaa gccaaacgca
     1201 aaagcaagga agttggcaat gttggaaaga tcattcatca ccatttccat ggcaagaagc
     1261 cagatcacaa tttcaaaaaa agcaataatc ggggcgagat acttgatgcc ttttgagata
     1321 tatatgaccc tgatggtctc catgctcaca tcgcagattc gggcgataaa tatgagaagg
     1381 gggagaatca cccatgaata gatctcagga ttaataatga aaaacgacaa tttttcatcc
     1441 tttctgcaca ggtgcagcat ttgtctgatg gttgatcaag gtcatctgtg tttgtatatc
     1501 aagataatcc agaaatatca aaaataagag aaatttggca ttgaatccga gatcacgcgg
     1561 attaataagc ctgtttcatt tacacatact tttgcggttc ggtgtcaaat ttttttttgc
     1621 agcccggagc acagaagtaa tactttttac cctgatactc gctggtaaac ttagccgttt
     1681 tttcatccac ggtcatcttg cagataggat cgattgccat gtttccacct cagttatctc
     1741 ccttgctttg cctcgggtat atactttttg agcagcagtg aaaggctgac cacagttacc
     1801 gagctcatgg ccatggcaag agcagcaagt tcgggcttaa acgtgaaacc aaaaaacgga
     1861 tagagcaccc ccgccccgac cggaatgagg gcggtgttgt aggcaaaagc ccagaagatg
     1921 ttacctttga tcctccccat cacttttttt gacagctgga tggctgccac cgcatcaagc
     1981 agatcatctt tgataagaac cacgtcgccg ctttcaattg caatatccgt tccgctgcca
     2041 atagcaattc ctacatctgc ccgcgcgagc gcaggtgcat cattgatccc gtcccccaca
     2101 aatgcgacga tctcgccttt ttcctggaga gccttcactt ccgcggcctt gtcctgcggc
     2161 agcacttccg caatgacccg gtcaatgcca atctgacggg atatcgcatc ggccgtacgt
     2221 ttgttatcac cggtgatcat gacaacctga attcccattt ttttgagttg ccggacagat
     2281 tccccgctcg tctccttgag agtatccgca atcgcgataa ttcccgctaa ctgcattccg
     2341 gcagcaataa gaatcgcggt ttttccatcc tgttcgaatg cagcaatctg aacttcgaca
     2401 gcatcgctga cggttactcc tttctcctgc atcagcagac gattgcctgc aaggacgttt
     2461 tctgaaagaa ccgttgccac gacacctttg ccaccgaaag tgtcaaaatg gtcagcctga
     2521 gggatactga ttccggcatt ttttgctttc cgcacaactg cctgtgccag agggtgctgg
     2581 gaattcatct caacaccagc ggttaaacca agcagcgttg attcggatat gccaaaagga
     2641 ataatatcgg ttacctcagg ttttcctttt gtaagtgtgc cggttttatc aaaaacaacc
     2701 gtcgtgactt tttcagcaat ttcgagggct tccccgtttt ttattaagat ccccagttct
     2761 gcaccccttc ccacgccgac tgtcacagca gtaggtgtcg ccaggccaag ggcgcaggga
     2821 caggctacca caagtactga tatcagtgca gtaagcgcaa acaacaatgt cgaattcagt
     2881 acaaagaacc acacgataaa agaaatacca gcaatcgcaa ttaccgcggg tataaagtag
     2941 gttactgcaa tatcagcgat tcgttgcacc ggtggttttg atccctgtgc atcctccacg
     3001 agctggatga tctgagcgag aaccgtttca cgccctactt tggtggcctt cacggaaaga
     3061 acgctgttgg tatttatggt gcctccaacc actcggcttc cgggtgattt aagaggagga
     3121 acaggttcac cggtgatcat tgattcatca acgtaacttg caccaccaac cacttcacca
     3181 tcaaccggca ccttgctgcc tggctttact acaacaatgt caccgacgac aacatcttca
     3241 acaggaattt cctcttccct gccttcacgg attatcgtgg cggtctttgc tcgaagcccg
     3301 gcaagtttcc ggatagcttc tgaagtcctc ccttttgccc ttgcctcaag gtaccgcccg
     3361 agcataagga atgatgcaag catgatcgct gtatcataaa acatgaactc atgggtgagg
     3421 atgataccga aggttcccat aacactcgca acaaatgcaa caccggtacc cattgcatac
     3481 attacatcca tattgagcga gcggttccgc agcgccatgg atgcagcacg gaaaatggga
     3541 taggcaacaa aatagaatac cggtgcggag acagccagca ttatatacgc aagggtgtgc
     3601 atggaaatgg ggaggggcac ccacatggca atcatgagcg gtatacttac ggcaaatcca
     3661 atggagaacc ggagaaattt gtcatgcaga tctttttccc gtgcaatctt ttctgcttcc
     3721 tcgcttacct ctccggcaat accaagatac tggtaacctg catcttcaat ggcttttttc
     3781 atctcgggaa ttgttgaaat tgaggggttg aaggctacat acgccttttc ggtaccaaga
     3841 tttacatttg cggatatgat accgggcagt gcccgaagcg cggtctctat tgtctgtaca
     3901 caggtagcac agaccatccc cccgattttt atggttactt cgtggtttac cacatcatac
     3961 ccggctgttt tcaccgcctt ttccagttcg gtcagcgaga cttttttcgg gtcaaactca
     4021 acatgtgctg tatcggtgcc aaaatttacc tgaacctttg agacatcctt tagctgcccc
     4081 agtgattctt caatattgat agcacatgta gcacagtgca ttccagaaat ttttaattct
     4141 gctttctttt tttccgtatc agccatgagg ataatctcct gaatcagatt ataatgaagc
     4201 catgacagcc attatttccg gattttgtta ccctgccatt gttggtgtgc cccgggaata
     4261 attgaaataa taaaattttc gctgattaac tgtttcttct ccggtattcc tcaaccagag
     4321 ccgttgcata tacttctgtt tttttcgtgg gcacgtaatt gaactctttt acccgtttta
     4381 acagctccgc agcgaggaca gcatcattat caggaccgag ctttttcact tcagaaaaga
     4441 tccagtccag gtggtcaata ccggtttcct tgccatcgat aagtacccgt ttcgtagtga
     4501 ttttgtccgg gggaattccc ccgcagatcg agcaataata cccgtcttta tcatctccac
     4561 ccatgttctc actccttgat catggtgagc gacatcttga atttcgtaat tgctgagagc
     4621 gcatgcggga cattggccgg gaagatgatc gtgtctcctg acttcatcgt gaaagtcttt
     4681 cctgcgaccc agacctcact ttcaccttca aggatagtaa ccaccgcatc gaatggtgca
     4741 gtatgctcag agagaccttc gttctcgtca aatgagaaga tcgtgatatt ccccgcttta
     4801 cgtgagataa tcatccggct tgcaacagta ccttcattat agttcaccag atcttttaac
     4861 tgcaggactt ttcctttcag ctcatcgcgt tttttgtcgg gtttatctga agtatccatt
     4921 ttgtattctg ccacgtaatc cctccactcc ctgattcagg tgcaccatgt tataatcctg
     4981 tcatttcagg gtaatatctt tacgccccaa accaacacgg cggctcatga attggaaatc
     5041 tttcatgatg tgaggctcta aaatcactgc tctgtagaat tcctctttat ccctgatgcc
     5101 tcactgggag gatgctggaa tatcccgcga ccggtgcccg cccgagtccc aaaccacatc
     5161 cctcctgctt tttggtcttt gtaacattgc tatattcgac ctcatacgtt cccgtcagaa
     5221 atcgttgaat aattccggcc cggttccgca tttttctaaa attatgacct cagaaatccg
     5281 gggggtgggg gaaatgaggt acaaccgggc tcgtagcagg ctccggtgta ccagaattct
     5341 cacagaagtt ttttgacggt aaaaaataac cccagatgac aataatatcc tgcctggtct
     5401 tcggtaagat tgctccttta aaacaggatt tcccatgaaa atcgattcat tcaggtatga
     5461 tgaggggatt atcagatcta gtcttcaggt acgatgaacg ctgccgcccc cgacggggcg
     5521 cccccacggc ggatcagtaa caaaaatttt aaacagccga cccgtcccac cgtgcctcgg
     5581 agaggccctg aggcggggtg catcatatgt tgtacgattt tcaggttcgg atgtgaattc
     5641 ccgttcatgt ctgtttctac tgagcaatta ttgtaaaaac attttgtatc aaattttttt
     5701 gtgttatttt ttcggcgatg attccagtaa caattccagc atccttggtg attcagtagt
     5761 cggacttgcg cagatatgac ctgaacaaat gtatgcagtt gccttcccgc ccattgcatg
     5821 catgttctgc gtgaagggag caatgatatt gagctggtca gagacggctc cgggtgaccg
     5881 gcagatgacc acgatgtgag gaaaataatg atcgtgaagc gcacgtatca gaattcctgt
     5941 atcctccgca tcctgttcac ctgcaataac aacatcctga agaggtccga taattgtatc
     6001 gagatcacac aggtaccatg tatgtgccga tggtgattgt tgaacagatg gcataaaaca
     6061 acgtaaaagt tcaaaagccc gctcttctgt ttttgtctca ccggtaagat gtgcaagccg
     6121 tatcaggttc tctaacgcaa cagaattaca tgatggaatt gccccatcgt aaaattcctt
     6181 tttgcgaatc aagagaactt cgccatcatc tgatatggtg aaaaaacctc ctttttcttt
     6241 gtcccaaaaa tgctcaataa acactgcatt cagttcaagt gccgcttgca ggtatgatgg
     6301 ttcaaacgta gattcataga gttcaatcag tgctttgaca atgaacgcat aatcgtcggc
     6361 aaatgccgga attgcaggtt cactatcacg ataccggtgc caaagccggc ccttggaatc
     6421 acgcatatgg gtcaggataa attccattgc ccttctcgca gctgtcaggt agtcctcgtt
     6481 tttgaaagtc cgggcagcct gagcaagcgc ggcgataaaa aggccattcc agtcagtcag
     6541 gattttatca tcaagggatg gtcgtgaccg atgagatcgt gccttaaaga gtctggccct
     6601 gacagagtcg agccgcagtt caggactggc ttttaataaa cctggtgaat attccgattc
     6661 ggtcatcgat ggttgaagaa aaaggatatt ctgtgcattc ccgccttctg cagggtgata
     6721 atttcccgat gttgtcacat tgaatgcacg cgtagcatac attgcgtcat cctttcctaa
     6781 aatattctcc atctcctgcg aggtccagag ataaaaagat ccttcgcctc ccccgctatc
     6841 ggcatcctct gctgaataga aggcaccctc tggagatatc atgtcacgaa gcgtgtaggt
     6901 gataatttct tgagcagttt tgcggaaagc atgcttcttt aaagcctgat acgcttcggt
     6961 atacgccatg acaaggagtg cctggtcata gagcattttt tcaaagtggg gcacgcgcca
     7021 ttgcatgtcg gtcgaatacc ggtgaaatcc tcctcctatc tgatcaaaaa tgccccccga
     7081 ttgcaactca tggagcgtct tttccaccat gaaaagtgca tgttcattac cggtacgcct
     7141 ccagaacctt aacaggaaga gcagagtatg cggagtggga aatttaggtg catggccgaa
     7201 tcctccattt tctgaatcaa accggagagc gagttcctcg tatccagaat tgagaagtga
     7261 aataccgggg attcctgcag gcatggtgtc ctgttcctga tgcagagcgg cggtaatctg
     7321 atctgccgat ctgaagagat cttctcgttt ctcatgccat aatctggtaa tacggggaat
     7381 caggtctatg agtccggtaa tgccaaaacg gctctccctg gggaaatatg taccggcaaa
     7441 gaatggtttc ttctcaaaag tcatgatgac ggtgagtggc catccccctt gcccggtcat
     7501 catctggcag actgacatgt aaatgctgtc gatatccgga cgttcctcac gatcaacctt
     7561 gatacagata aaatccctgt tcaataaagc agcaataccg ttgtcctcaa aggattcatg
     7621 cgccatcacg tgacaccagt gacaggtagc atacccgatg gaaagaaaca cgggtttatc
     7681 ttcatgtgcc gcccgcagga aggctccggc accccatgga taccagtcga ccgggttcgt
     7741 tgcgtgctgg aggaggtaag gacttttttc atgaataaga cggttgtact tcacgtcatc
     7801 ggtaacaggt gattgaccct gtttgcggtt ctccagcgtc atatctgccc atttgcattt
     7861 taagatgatg aggctttcag atgcagtctg gtgacagcgg tgaagttatg tgctcgctgg
     7921 aaccaaacaa agtaggaata atggtcttga ctgccgttcc atttcttacg gttattttta
     7981 taggcattat cgtaatactc atctcccgta cacttgatgt cctgtgggct caggttctcc
     8041 cgatgaggtt tttttattcc ctgctccgtg cccccggagt tatcattcac gaattaagtc
     8101 atgtgctggg atgcctgatc agcggggcaa aagtaaaaaa agtcatatat ttttccaatg
     8161 agggtggatc tgtgacgtat tcatccccga aaatccctta tattggtgac cttgtgatta
     8221 acatcgcacc gcttttctgc atccctctta tactggccgg gtgcacctgg atcttttcag
     8281 aataccttgg gtgcgttttt cccccaatgc ctatgggagt caattcactg gacgcacttt
     8341 ttggagtggt taccggtatt cttggcatgt tcacttcaaa tcttattatc cgtttcaatc
     8401 catggttcct tgcctacctg tacctcaatc tgacgcttgt gctgtcggtt gccccgagtt
     8461 cccaggatat aaaaaatgcg gcggtcggga tcggtgttat cactctgatg ggtatgatga
     8521 tcctctggag ttccaatact cttgcaggaa acctccttga cggatttgtg tacctcctcg
     8581 gtattggttt ttccctgggt ctgctgtttg ggattatcgc actcgtgatc tccagtcctc
     8641 tggtaatcct gtatgttcat aaaaaaattt aagaaaccaa ccgggcaggg tcattagatc
     8701 ggtattaatt gatcaaaaag gctatttttc tgcaggtatg attcacgagt ctttccccga
     8761 tcgttctcca ggtccggaga atggcgttcg tctcacgccg gtaatgcggc agtatcatga
     8821 gatcaaggat aagtacccgg ataccatact ttttttccgt attggtgatt tctatgagac
     8881 ctttgaaagc gatgccgagc ttatatcccg ggaactggag atagttctta cctctcgttc
     8941 aaaaagcggg gatacgaaga tacctcttgc aggagtgcct tatcattctg cggacggata
     9001 tatcgccaaa cttgtgggaa agggctacaa agtggccatt gttgatcagg tcggagacac
     9061 aaaaaacaca aaagggatcg ttaaacgtga gctggtacgg gtgattacgc caggtacggt
     9121 aatcgactct gctatgcttt cttcatcggg ctcatcaagt ctgctcgcaa tctgtcctga
     9181 cagaaaaaac agtcattggg gaattgcgct tctcgatata tccacggggg aattttttgt
     9241 aacactcgta gatcacgata agggtttttt gaatattctt tcggaaattg cccgcaaccg
     9301 tcctgccgaa tgtatcgtcc cttcaactat tactgatgac ctcaagcaac gaattcgtga
     9361 cagggatgtt gtggtcacct gcctggacga tcgggaattc tccgaacagc gggcacggga
     9421 cactcttttg gaacacttcc acgttgtctc acttgccggt tatggatgtg atgattttcc
     9481 agcggcaatc tctgcagccg gcgcggcact ttcgtatgcc aaagagaccc agagatcgtc
     9541 attgactcat atcagcagtc tcgttttacg ttcatctgaa cagggtctga tgctggatgc
     9601 aatcacactc cgtaaccttg aaattaagga aagcatccgg catgatacaa aaggagcaac
     9661 gcttctctct tgtctggacc tgaccaagac atcgatggga agcagggtgc ttgcccagcg
     9721 tctgtcacgt cccattaccg gtattgaaga gatcaataaa aggctggacg cgattgaatt
     9781 ttttatagac aatacacagg tgcgacttgc agtccgttcc ctgctttcac ggtttgccga
     9841 tatcgagagg attgcggcaa gaattgcgta cgggaatgcc gggccccggg atctgctggc
     9901 tctggcagaa tccctcgaat ccattccgga ccttaagaat tcatttcagg ttttccgtgc
     9961 tcctgatgta cccgatcaga ttatgaacgc ccttaacgat ctcaatgatt tgcccgacag
    10021 cattcatctc atccggggag caattgttga tgatcctcct gcaattgcac gcaacggtgg
    10081 ggttatcagg cagggttttt ctcaggatct ggacactatc aaaggtgtcc tgcactcagg
    10141 aaagggctgg attttggagc tcgagcagaa agagcgtgaa aagacgggga ttaaatctct
    10201 caagatcggg tataacagga tttttggtta ttatatcgat atcacaaaac caaatcttca
    10261 cctggttccc tctcattatg agcgtcgtca gacaacggca accggagagc gctatatact
    10321 ccctgaactc aaggaaaaag agacccttat aactaatgca gatgagcgtg tccttgcact
    10381 ggaacgagaa ctctatgggg gactggttga aacattacgc accataattc ctgaactcca
    10441 ggcaatcgcc cgtgggactg ctctcatgga tgtgtcagct tcccttgcag aaattgccaa
    10501 aacccatgat tatgtacgcc cgcagttgaa ttcgggtgat gcgatcatta taagagatgg
    10561 acggcatccg gttgtcgaac agggtgcttt tggagatttt gtaccaaacg ataccgatct
    10621 gtccagttcg ggcacgcagg taatgataat taccggagca aacatggcgg gaaagtccac
    10681 gtacatgcgg gcggttgcgc ttatctgtat aatggcccag gcaggaagtt ttgtgcctgc
    10741 acggcacgcc agtatcggaa ttcttgatcg catattcacc cgtgttggcg catttgatga
    10801 tctcgcaagc ggacagagta cgttttttgt cgagatgctc gaacttgcta atattctcaa
    10861 caacgtgacg ccaagaagtc ttgtaatcct cgatgaaatt ggccggggaa cgagtacagt
    10921 tgacgggtgc tcaattgcaa aggcggtact ggaatacctt cacggcagac ccggtgccgc
    10981 cccaaaaacc ctgtttgcca cgcacttcca tgaacttgtt gaagtcgaag gtgtactaaa
    11041 aagagtaaaa aattatcatt ttgccgtaaa agagacaaag aatgatgtga ttttcctgcg
    11101 gaaactcatt cccggggcga ctgataaaag ttatgggatt catgttgccc ggcttgccgg
    11161 tatcccgaaa aaagtgactg agcgtgctga tagtattctg actgagacca tgaaccgtga
    11221 agtcccttcc ggctcccgcc cccagcgtta tacccagatc cttttgttcg ataatgagaa
    11281 ccctcagggc cccgtcacaa cacacccggc cctcgaaaaa cttgccggta tcaatcctga
    11341 cgagatcact cccctgcagg cgcttgcaat tattgccgag ttacggaaga tgctgaatcg
    11401 gaaaggaaac aaaccatgag gaccaccatc cgcatccttg atcaggcaac ggtcaacaag
    11461 attgcagcag gtgagatagt tgaacgccct gcatcggtag taaaagaact ggttgaaaat
    11521 gcgattgatg ccggggctca tgtcatacgc atggatattt catcctcatc agaagggatc
    11581 gaaagtatcc ggataactga tgatggcatg gggatgtctc cggatgatgc agtgtttgcc
    11641 ttcacgccgc atgcaaccag caagatttcc ggaatcgagg atctcaatca tataaccaca
    11701 ctcggttttc gtggtgaagc tcttgccagt attgcagcag tcgcacggat cacactgatc
    11761 acccgacttc gtggcaccag tgtttctgga acaaaagtgg ttgttgaagg tgggaaatta
    11821 ctggcaaaat cagatttcgg agcacctgaa gggaccagtg tcctcgttga agatcttttc
    11881 tacaatactc cagcacgaaa aaaattccag aagagtaaaa acaccgagat tggacatatt
    11941 cacgcacttt tagagggtat ctgcctatcc cacccggaga tctcatttcg tctgttcttc
    12001 aatcgccgtg agcagctggt gacggaccgt acggctctcc tcctcgatac cattgcccgc
    12061 ctctatggaa atgatatggt aaaagacctc gtccccgtac atctgtcgct ccccttcatg
    12121 aaaatttccg ggtacacctc acgcccctcg tttacccgaa aggatccatc tcggatgatg
    12181 ataacgatca atcaacggta tgtatcttca gcaatagtca ctggtgccat caaggacggg
    12241 tatggttctt tactcccaaa agatctgttt ccagctgctt tcctttccct gacgattgat
    12301 accagccttg tcgatgtaaa tgtacacccc acaaaaaaac aggtccgtct ttcaagggaa
    12361 ggagagatca cccaggcaat atgtgacgct gtgaaaacgg cattattgag ccaggatctc
    12421 atccctcata taggtcaacc gtcagatgtg tacatgagtg aagactgcag gaataatgaa
    12481 gctggatcgg acacgcatta tgatttttcc ggattacttc ccgaaggaat cagggaaacg
    12541 gtgcatgcag gaactttttt gactgaccgg caattgagac agaccgaact cagtacagtc
    12601 attacaccgg tcgatgtaca acttccagcc atggaggtta tcggacaatt cggaggaatt
    12661 tatatcctgg caacaactga tgccggtgaa ttgatactta ttgaccagca cgcggttcac
    12721 gaacgtatac tttacgagca ggtctccaca cgaaccgaag gcatccatcg ttcacaggaa
    12781 ttgatcgtcc ctgtactcct gcaccgtact ctcaaagatg cctctatcct tcgggatatt
    12841 attccggcac ttgcccggga aggcttcatt cttgaggaat ttggcaagga tacattcctg
    12901 gtacgtgcaa tccctgtagt cctggggaag ctcgaggaca caacgatgat cgatgacatg
    12961 gttagtgatc ttgtcaatga tgaggcggta agaactgtaa ataaccggga aaaaattgcc
    13021 cgcattatcg catgtcgcgg tgccataaaa gcaggaacgg tctgttcacg tgagcagtgc
    13081 cagcgcctga ttgagcagat ccggtatgta aaaaatccct tcacatgtcc tcatggccgg
    13141 ccgactatga tcaggttcag cagg
//
LOCUS       DASRLV010000208         4141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_892258,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000208 DASRLV010000000
VERSION     DASRLV010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892258"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VFC43_08000"
     CDS             complement(<1..416)
                     /locus_tag="VFC43_08000"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HZK31154.1"
                     /translation="MKRNLENRPPVQAGKNDSSIELKTKEKAIIMSGYGINSEMETQE
                     AFKRAGMDSDIVHINDLIAGRKRVSDYRLLVFPGGFSYGDDTGAGNAFANRVRNNLWR
                     DVENFLDADNLVLGICNGFQILANLGLVPAFNKSYER"
     gene            complement(424..3366)
                     /locus_tag="VFC43_08005"
     CDS             complement(424..3366)
                     /locus_tag="VFC43_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HZK31155.1"
                     /translation="MTSFIHRIEVHYKQDPRLNSRTDRIRSLGIPVRELHLIDIYTIA
                     TSSRDFTHDELCRIGAQLINPVIQDYTIDSATTADFDYAIEVGFLPGVTDNVGMTAKQ
                     TIEDFFSIRFKEGESVYTSQLYFIFGKLSENSLQKLAATLANPLVNRLHIRTFEEYGS
                     EGMDTCIPSVRLHELPTAEAVDLDLDDKDLARLGKEGIPDSVTGQPRGPLALDLSQLY
                     AIRTYFSIQGRKPTDVELESLAQTWSEHCKHTIFASSMDDDVPKGLYKTFIQEATNKI
                     RREKGDKDICVTVFTDNSGAIIFDDNYLVTHKVETHNSPSALDPFGGALTGIVGVNRD
                     TIGFGLGAKPCINYYGYCVGDPDSAPVLFRGKNKANPILSPRQILDGVVRGVGVGGNC
                     SGIPTPQGWCYFHDRYTGKPLVFAGTVGVMPLRHRGRKLFEKQAKPGDLIVMAGGRVG
                     KDGIHGATFSSEALDPASPVTAVQIGDPITQKKLSDVLVREARDLGLYHSITDNGAGG
                     ISCSVAEMAKECNGCHVFLDQVPLKYHGMAPWEIWISESQERMTLAIPKEKLSEFMDL
                     VQRREVEATVIGKFTDSGRCVVEYNGNIVMDIDLDFLHDGLPKKHLRTTYTQKKYPEP
                     EFVCPPRLDKIILSMLQRKNICSKEFISTQYDHTVQGGHVLGPFQGKGRVQATSTLTR
                     VVLDSEKGVGLSQGIFPLYAEIDPYLMAAAAIDTAIRGLIAIGISLDNIALLDNFCWC
                     SSDEPERLGQLKLAAKGCYDFATAFGTPFISGKDSMFNDFSGFDAENNTVRISVPPTL
                     LISSIGVHDNVEKAVSIDAKFEGDFVYVIGETVPELGGSEYFAFLDSTGNIVPKMDTK
                     KLKSRYQRLNAAIGKEIVASAYPVNHGGLAIALGKVAIAGQIGMEITIPSETRPDYFL
                     FSESLGRFIVTVSKDNKKEFEEIMGGDAMVLGTVCGRMLRITAQKIIADIPVEELETA
                     YKSPFIGY"
     gene            3521..4138
                     /locus_tag="VFC43_08010"
     CDS             3521..4138
                     /locus_tag="VFC43_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit beta"
                     /protein_id="HZK31156.1"
                     /translation="MTDEYENLLKQAYSNITEKTESSERFVVPEAKAYVEGKTTVLEN
                     FSEIAAKVRRDQEHLMKFLLGELGTSGKIDGNRAIFNGKFEISLIKMMIKSYIEDYVI
                     CSECGKPDTRLVKDDRVMQLRCDACGSHRPVRKRKARTEPVSENLEEGTVMEVEIQST
                     SKRGDGVVKMGRYIMYVANAKPGQKVKIKISRISGSIVFTERAEE"
BASE COUNT         1098 a          994 c          902 g         1147 t
ORIGIN      
        1 cgttcatagc tcttattgaa cgccggaaca agaccgaggt ttgcaagaat ctgaaaccca
       61 ttacaaattc ctaaaaccag gttatcagcg tccaaaaagt tctccacgtc ccgccacagg
      121 ttgtttcgga ccctgttggc aaatgcatta ccggctccgg tatcgtcccc gtaagaaaat
      181 ccacctggaa acacaaggag acgataatcg gatacccttt ttcttcctgc tatcaggtcg
      241 tttatatgaa caatatcaga atccatccct gcccgtttga atgcttcctg tgtttccatc
      301 tctgaattaa tgccatatcc actcattatt atggcctttt ccttagtttt caactcaatt
      361 gatgaatcgt tctttccagc ctgcacgggg ggtctattct ccagattacg tttcatgttc
      421 attttaatac ccgatgaaag gtgatttgta ggcagtctca agttcctcaa cggggatatc
      481 agcaattatt ttttgtgcgg tgattcgtag catcctgccg caaaccgtac cgagcaccat
      541 cgcatcacca cccattatct cttcaaattc cttcttattg tctttggaaa ccgttacaat
      601 aaatctcccc agagattctg aaaacaggaa ataatccgga cgtgtttctg aaggtatagt
      661 tatttccatt ccaatctggc cagcaatggc gactttccca agtgcaatcg caagaccccc
      721 atgattgaca ggatatgcag atgccacaat ctcttttcct attgcagcat tcaaccgctg
      781 ataccgggat tttagttttt ttgtatccat tttcggaaca atgtttcctg tactgtccag
      841 gaaagcgaaa tactccgatc cccccagttc cggaactgtt tccccgatta cgtaaacaaa
      901 atcaccctcg aatttcgcat cgatagacac tgccttttct acgttatcat gaactcctat
      961 tgaagagata aggagtgtcg ggggaactga tatcctgaca gtattgtttt cggcatcaaa
     1021 cccggagaaa tcgttgaaca tactgtcttt tcctgaaatg aatggagtgc caaatgcagt
     1081 agcaaaatcg taacaccctt ttgctgccag cttcagttga ccaagtcgct ccggctcatc
     1141 ggatgagcac cagcagaaat tatcaagaag tgcgatatta tccaaagata tacctattgc
     1201 aataagtccc cttatggcgg tgtcaatcgc cgcagcggcc atgagataag gatcaatttc
     1261 cgcatataaa gggaagatac cctgcgacaa tccaacccct ttttcagaat cgagtacaac
     1321 cctggttaat gtggaagttg cctgcacacg tcctttaccc tggaaaggtc caaggacgtg
     1381 gcctccctgg actgtatggt cgtactgcgt tgatataaac tctttcgagc agatattttt
     1441 cctttgcagc atggacagga ttattttatc cagccttggt ggacagacaa attccggttc
     1501 cgggtacttt ttttgtgtat aggtagtcct gagatgtttc tttggcaaac cgtcatgcag
     1561 aaaatccagg tcaatatcca tcacgatgtt accgttatac tcgacaacac atctgccgct
     1621 gtcagtgaat tttccgataa ccgttgcctc tacctcacgc cgctgaacca gatccataaa
     1681 ttcggagagt ttctcttttg ggatcgcaag ggtcattcgc tcctgtgatt cagagatcca
     1741 gatctcccat ggcgccatcc catgatattt taatggtacc tggtcgagga agacatggca
     1801 accgttgcac tcttttgcca tctctgctac tgagcaggaa ataccacctg ccccattatc
     1861 tgttatacta tggtaaagcc cgagatcccg ggcttcccgg actaaaacat cggataactt
     1921 tttctgggta atcgggtcac caatctggac tgcggtgacc gggctggctg gatccagcgc
     1981 ttctgaggaa aacgtggcac cgtggatgcc atcttttcct acccgacccc cggccattac
     2041 tatcaggtca ccgggttttg cctgtttttc aaagagtttc ctgcctctgt gtctgagtgg
     2101 cattaccccg acggtacctg caaacacgag tggtttaccg gtataccggt catggaaata
     2161 acaccagccc tgtggggttg gtattcctga acagttacct ccgacaccca ccccccttac
     2221 taccccgtca aggatctgac ggggagaaag tattggattt gccttgtttt ttccacgaaa
     2281 cagtaccggg gctgagtctg gatctccaac gcagtaccca taataattga tacagggctt
     2341 tgccccgaga ccaaaaccaa tcgtatcacg attcacacct acaatcccgg tcagagcccc
     2401 gccaaatgga tccagagctg atggagagtt gtgggtctcg actttatggg ttacaaggta
     2461 attgtcatcg aagataatcg ccccggaatt gtctgtaaaa acggtcacgc aaatatcctt
     2521 atctcccttt tcccggcgga ttttatttgt tgcttcctgg atgaatgttt tgtagagccc
     2581 ttttggaacg tcgtcatcca tcgatgaagc aaagattgta tgcttgcaat gctcgctcca
     2641 cgtctgtgcg agagactcca gttcaacatc tgttggtttt cttccctgta tggaaaaata
     2701 ggtacggatt gcataaagct gtgagaggtc gagtgcaagc ggtcctcttg gttgaccggt
     2761 aacagaatcc ggtattcctt cttttcccaa ccgggccaga tctttgtcat ccaaatcaag
     2821 atcaactgct tctgcagtcg ggagctcatg taaccggaca gagggaatgc atgtatccat
     2881 cccctcactt ccatattcct cgaatgtcct gatatgaagt cggttcacca gtggatttgc
     2941 aagagttgct gctaatttct gcagggaatt ctctgaaagt tttccgaaaa taaaataaag
     3001 ctgggaagta tagaccgatt ccccctcttt aaaccttatt gaaaaaaaat cttcaatggt
     3061 ctgctttgcc gtcataccaa cattatcagt aacgcctggg agaaatccca cctcgattgc
     3121 gtaatcaaaa tcagcagtcg tcgctgaatc tatggtataa tcctgaataa cggggttaat
     3181 aagttgtgca ccaatccggc aaagttcatc atgggtaaaa tctctcgaac ttgtggcaat
     3241 tgtgtagatg tcgatgagat gcagttcccg gactggtata cctaaagagc ggattctgtc
     3301 ggtcctggaa tttaaccggg gatcctgttt atagtgaact tctatccggt ggataaaaga
     3361 agtcatgatc actagttaat cgtgtaatta caaaatatct gttatacaga cctgtggccg
     3421 aaatttgttt ttcccttttc gtcagaacga tccgatcatc tgtatccccg ccccaattgg
     3481 gaaagccttt tggcgggtgg cacctttata ctatacaact atgacggacg aatatgaaaa
     3541 tctcctcaaa caggcatact ccaatatcac tgaaaaaaca gaatcttcag aacgttttgt
     3601 agttcctgaa gcaaaggcat atgttgaggg taaaactacc gttcttgaaa atttttctga
     3661 gattgcagcc aaggttcgcc gggatcagga acatctgatg aaattccttc ttggtgaatt
     3721 gggaacatca ggaaaaatcg atggaaaccg tgccattttc aatgggaaat tcgagatctc
     3781 actcattaaa atgatgatca agagctatat agaggactat gtgatctgct ctgaatgcgg
     3841 gaaaccagat acccggctgg tgaaagatga tcgggtgatg cagctccgtt gtgatgcttg
     3901 tgggagccat cgcccggtaa gaaagagaaa agcccgcacc gaacctgttt cagagaacct
     3961 tgaagaaggt acggtcatgg aagtggagat ccagtcaaca tcaaaacggg gagacggggt
     4021 tgtcaagatg ggccgttata tcatgtacgt ggcaaatgca aagcccggac agaaggtaaa
     4081 aatcaagatt tcccgtatct ccggatcgat tgtatttacg gaacgagctg aggaatagtt
     4141 t
//
LOCUS       DASRLV010000209        10009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_239032,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000209 DASRLV010000000
VERSION     DASRLV010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 10009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10009
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239032"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VFC43_08015"
     CDS             complement(<1..396)
                     /locus_tag="VFC43_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZK31157.1"
                     /translation="MEMKYVPTTCPYCGTGCGLNLVVVDKKVVGVAPWHRNPVNDGKL
                     CPKGNYAAEFINSPDRLTKPLIKKDGKFVEASWDEAYNLIASKFKSYKGEEMACLASA
                     RVSNEENYLMQKFARAVLKTPNIDHCARLC"
     gene            593..1222
                     /locus_tag="VFC43_08020"
     CDS             593..1222
                     /locus_tag="VFC43_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31158.1"
                     /translation="MKQPRIPRLIVDVIQNALLSCDGVIFDTREACPVCGGKLSGYDT
                     KKKQFAVMLEGDDRHPVHVLVKRFSCRQCKAVCLADEPFYPDTRIGSPVVDLCRTLTG
                     GMSMYRATAFIRHLGIMVERGTVRNYAQGSYKEIPTTELYGLRLPLSVISLSELTARS
                     GEGSRIPGTEVLAACGFPSAYRAAPDHRATPEQGDERDNEKDKEKRQAK"
     gene            complement(1350..2288)
                     /locus_tag="VFC43_08025"
     CDS             complement(1350..2288)
                     /locus_tag="VFC43_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HZK31159.1"
                     /translation="MIGIDVGGANLKVVSDDGVCIHYCPLWEHAPVTTLLEQYATRHG
                     EQAAAVVMSGELADCFENKMDGISFIVNAVKDAFASAHFYGTDGRFHDNAVPQLAAAN
                     WLASADFLRARYPDAVLLDIGSTTADIIPLCCFESLKGLTDLFRLQKGYLVYTGMLRG
                     NIATLLQSVVLDSVPTPTSSEYFATSADAHLVLGHITPEQYSCDTPDKKEKTSIAAFR
                     RLARVVCADLEETGTDGAYQVAVQFWEQQKSLVCDQVRRVVAESGARQILVAGIGAPL
                     FARELEGIELSKELGSYADALPAFAVREVAVREKLR"
     gene            complement(2285..3172)
                     /locus_tag="VFC43_08030"
     CDS             complement(2285..3172)
                     /locus_tag="VFC43_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZK31160.1"
                     /translation="MKVLLAEYASAHDPTLAAEGAAMLDVLKQSFERRGYEVVLPGAG
                     EFGVEIERLAPTCSMGLVIAPDHLLAGYTQILEQHTHNLGCGFMTVALCANKVRTEKI
                     LRSHGIPVPAESPGGRRVIKPVRGCGAQGVRISDALPGKDEFSQHYIEGEHFSVSIVA
                     NRVIGDACLYFSGKPPLVLAVNRQFIETGSDGALHYRGGETPVHPVREPEIIEVAKKT
                     AEVLGCQGYCGVDVVVADTVYVVDVNPRITTSLVGIAACMTEEIADILVSASKGEGTD
                     RVHLIGSARFDTNGNVLSV"
     gene            3254..4039
                     /gene="surE"
                     /locus_tag="VFC43_08035"
     CDS             3254..4039
                     /gene="surE"
                     /locus_tag="VFC43_08035"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HZK31161.1"
                     /translation="MKPSILLTNDDGVSSVGLWAAYEALAPVADVTVVAPATQQSAVG
                     RSISIFEPLRASQITINGIPAWSVAGKPTDAVIIGLYALKLNPDLIVSGINIGENLSF
                     ESIMTSGTVGAALEGSNQGTKGIAFSLQVEDQGDKFDDPRHNGQDFEAAKNVVRDVVS
                     RILDHKFCPHADVINVNIPSVVKGGYEITRLARKLFLTGVEKRVDPRGRPYFWINGPL
                     LEDAEEGTDVHAIRKGNISITPITLDCTAYAAEKEARDIFYND"
     gene            4192..4905
                     /gene="rpiA"
                     /locus_tag="VFC43_08040"
     CDS             4192..4905
                     /gene="rpiA"
                     /locus_tag="VFC43_08040"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HZK31162.1"
                     /translation="MDNSAEALIAAKRAAGFEAAEMVKDGMVLGLGTGSTVFFALERL
                     SCRIKEGLQVSGVPTSFQTARLSREYGIPLTTLDEDPVLDLAVDGADQVDKHLFLIKG
                     RGAALTREKCVASAALSFLVIIDEQKFVSSLTAPVPVEVIPFAARPVMNQLEDLGCTA
                     ILREGIKKDGPVITDNGNFVVDCSFSEIDDPEELEKTIKMLHGVVDSGLFCGFTGKTT
                     VIVGNQKRCRVINSPDIIA"
     gene            complement(4885..5259)
                     /locus_tag="VFC43_08045"
     CDS             complement(4885..5259)
                     /locus_tag="VFC43_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HZK31163.1"
                     /translation="MRTESVMYFTEKEEEFANLLIEIGTKRNVAKVLVFLANTPEATS
                     RAIERGTDLRQPEVSIAMRYLIEQSWITSRESKAESKGRPVKIYVLAKPIHEIMDSIE
                     KEKKKEATTQLALVQKLRDYIR"
     gene            complement(5445..6005)
                     /locus_tag="VFC43_08050"
     CDS             complement(5445..6005)
                     /locus_tag="VFC43_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HZK31164.1"
                     /translation="MKVRPEDSLKIENIVASAKVTDYLDLPEIASKIPGAEYNKKRFP
                     GVVLRMQDPKIAALVFGSGKVVLTGAKSIDSLSKGLEILGGLLRKLGIAIPKKLDYKI
                     QNIVTSADLATPINLNKIAVGFNLDKIEYEPEQFPGLVYRLDTPKVVVLLFGSGKLII
                     TGGKEPEDAKKAVVKILSDLRSLGLI"
     gene            complement(6505..6819)
                     /locus_tag="VFC43_08055"
     CDS             complement(6505..6819)
                     /locus_tag="VFC43_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor S"
                     /protein_id="HZK31165.1"
                     /translation="MFCPECKSLLISSGGQLKCRKCGYIRKIEDADNMKHEVIGKERE
                     IWIVDDEEEKMKTMPTIQIKCPKCANNLAIWWLRQLRAADESEVRFFRCTKCEHTWRQ
                     YD"
     gene            6986..7795
                     /gene="artA"
                     /locus_tag="VFC43_08060"
     CDS             6986..7795
                     /gene="artA"
                     /locus_tag="VFC43_08060"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase A"
                     /protein_id="HZK31166.1"
                     /translation="MIEYLVLISCVGFLAFLIPGRHQKYAAIIGWTFIVLFLFAELPY
                     YFSLNNFLYPIMAALSLPFLIITSRYLLVDDDRVMHLSRAAAVAFLIYAPFEYLPLLG
                     DWLIGIVVGQVVWMLDALTYTVSLADWNIISRNTFRVEIILACTGIQSIAIMLGVAAA
                     VPTTTRQKIYAFVIIAPTIYILNLFRNVFVIIAYTEQWFPYYPAIASNGEFGYESFFW
                     AHNVIAELLALVCLVIIAYGLFTLIPNLGKFADSLYKAYSSEIRKAFGKDR"
     gene            complement(7767..8399)
                     /locus_tag="VFC43_08065"
     CDS             complement(7767..8399)
                     /locus_tag="VFC43_08065"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZK31167.1"
                     /translation="MNITALRPRFIRYLEPVADIFVKIRITPNQISVLSLLAGIVCAY
                     LFFRQQFIWGTLALILSAVFDLVDGSVARKTNSHSNFGAVFDWIVDKYVDGLVLLGIG
                     LSGISIVSQYITVPSFADFAIVALAIIGSLMNTFIKPVVYAEIGYRDKVEGKIDDPLE
                     GVGFFGRPETFLFLFLGGISGFIGVAVIIIAVCTNLSAIQRIIYLYRTLS"
     gene            8576..9262
                     /locus_tag="VFC43_08070"
     CDS             8576..9262
                     /locus_tag="VFC43_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HZK31168.1"
                     /translation="MTKTTDRPHVLMMSEITADGKLTLKKGASSKILMKYMSHETEIL
                     LHTTRAEYDAIMVGANTIRIDNSFLTVRLVPGKSPLRVIPCSMADLPLDANVLGKDAP
                     TAIAVCEAAPEDRVEAIMRKGAHVIVAGKNHVDLPLLLSILKKKFGIQKLMIEGGPTL
                     NWHMLHDKLVDEIRLIHLPFIVGGADTPSLVGGMHINSEDEMIRLNLKKYYMCGSNLV
                     SEFDILYHGA"
     gene            9265..>10009
                     /gene="sppA"
                     /locus_tag="VFC43_08075"
     CDS             9265..>10009
                     /gene="sppA"
                     /locus_tag="VFC43_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HZK31169.1"
                     /translation="MNGITPPPVGNHPQAHPGIKWFFIIIFGIIFVFGLLIAVYFFAG
                     NDQMGVTVIRMEGTMVTGQVSDAETIGSEVVGNELRDAADDPMVEAIVLRVNSPGGTP
                     AAAQEIIRDLDYAKSKKPVIVSMGDIGTSAAYYVSAHADRIYANPDTFTAGIGVIWKF
                     SDISRWMEKEGYNISVVKSGTKKDMGTTSRPISDEEQIYAQQIVNESFENFISDVTSQ
                     RPIARSDIEDGRVIRGTDAVRLHIVDEIGN"
BASE COUNT         2555 a         2514 c         2375 g         2565 t
ORIGIN      
        1 gcagagacgg gcgcagtggt cgatgttggg ggtctttaaa acagcccggg caaacttctg
       61 catcaggtag ttctcttcgt tggagacacg ggctgatgcc aggcatgcca tctcttcgcc
      121 cttgtatgac ttgaacttgg acgcgataag attgtatgcc tcgtcccagc tcgcctcaac
      181 aaatttccca tccttcttga tgagcggctt tgtcaggcgg tccgggctgt tgataaattc
      241 tgcagcatag ttgcccttcg ggcagagctt gccgtcattg accggatttc ggtgccacgg
      301 tgcaacgcca acaaccttct tgtccacgac aacaaggttt aagccgcaac cggtaccgca
      361 atacgggcat gtggttggta cgtacttcat ttccatgttc taacctcgaa caacaaaatt
      421 tttggttttg ccttatttaa acctagtgaa ttttttgcca aaagtgatta atagatgtgg
      481 gtagtaacct gacctctgat aatgcaatga aaaatgatat caataatatg tgttacaaag
      541 tggacggtgt taataattat tatctaaccc tgacaggatg ttcgcgcaca ttatgaaaca
      601 accacgcata ccccgcctca tcgtggatgt tatacagaat gcactgttgt catgcgacgg
      661 ggtaattttt gatacgcgtg aagcgtgtcc cgtttgtggc ggaaaactct ccggctatga
      721 tacaaaaaag aagcagtttg ccgtgatgct tgaaggggac gaccggcatc ctgttcatgt
      781 tctggtgaaa cgcttctcct gcaggcaatg caaggccgta tgccttgctg atgaaccatt
      841 ctacccggac acccggatcg gctctcctgt tgttgacctc tgccggacac ttaccggcgg
      901 aatgtcaatg tatcgtgcaa ctgcattcat ccggcatctg ggaattatgg tagaacgtgg
      961 aaccgtgcgc aactatgcac agggatcgta taaggaaatt cccaccacgg aactgtacgg
     1021 gcttcgtctt cccttgtcgg tcatctcact ttctgaactc actgcccgct ccggtgaggg
     1081 gagtcgcatc ccagggacag aagtgctcgc tgcctgtggt ttcccatccg cataccgggc
     1141 agcgccggat catagggcga cgccggaaca gggggatgag cgggacaacg agaaagacaa
     1201 agaaaaacgg caggccaagt aaccagccgg ctgccgtaac cgcgagggat cccaaaagaa
     1261 gaatgaggct caggtgccat gatttcatca ggcgatcacc ccatgacacg cccaagatac
     1321 cttggtgata gaacccggcc tgtacatatc taacgcagtt tctcccgtac agccacctca
     1381 cggacagcaa acgcaggcag ggcatctgca taagatccca gctctttaga gagttcaatc
     1441 ccctcaagtt cccgggcaaa caatggcgct ccaatgccgg caacgaggat ctgccttgca
     1501 ccggattctg caacaacacg ccgtacctgg tcacagacca ggcttttttg ttgttcccag
     1561 aactgtacag ctacctggta tgccccgtca gtacccgtct cctcaagatc cgcacagact
     1621 acccgggcaa gccgccggaa tgccgctatc gaagtctttt ctttcttatc gggagtgtca
     1681 caactatact gttccggagt gatgtgcccg aggaccaggt gggcatctgc actggtggca
     1741 aaatattcag agcttgtagg agttggcact gaatcaagca ccaccgattg cagcagggtg
     1801 gcaatatttc cccgcagcat gccggtatat acaagatatc ccttctggag cctgaaaaga
     1861 tcggtgagac cttttaagct ctcaaaacag caaagcggga taatatctgc cgttgtactg
     1921 ccgatatcga gaagcacggc atcagggtac cttgcccgta agaaatctgc agaggccagc
     1981 cagtttgcag cagcgagctg aggcacggcg ttatcgtgaa atctgccatc agtaccataa
     2041 aagtgtgctg acgcaaatgc atccttcacc gcattcacga taaacgagat cccatccatc
     2101 ttgttctcaa aacagtctgc cagttctccg ctcatgacaa cagctgctgc ctgttcaccg
     2161 tgtcgggtgg catactgctc cagcagtgtc gtaacggggg catgttccca gagcggacaa
     2221 tagtggatgc agacaccgtc atcgctcacc actttgaggt ttgcacctcc gacatcaatg
     2281 ccaatcatac gctcaaaaca tttccgttcg tgtcaaaacg ggcactgccg atcaggtgaa
     2341 cacggtctgt gccctcccct ttcgatgccg aaaccaaaat atcggcaatc tcttctgtca
     2401 tgcaggcagc aatgccaacc aggctcgtcg taatccgcgg attcacatcc accacgtaca
     2461 cggtatcggc aaccacaacg tcgacaccac aatatccctg gcaaccgagg acttctgcgg
     2521 tctttttggc cacctcaata atttcaggct cccggacagg atggactggc gtctcccccc
     2581 cgcgataatg aagtgcccca tcgcttcccg tctctataaa ctgacggttt accgcgagca
     2641 caagcggggg tttaccggag aaatagagac aggcatcgcc gattacccgg ttggcaacaa
     2701 tgctgacaga gaaatgttct ccctcaatat agtgctggga aaattcatcc ttaccgggta
     2761 gtgcatcgga tattctcaca ccctgcgcgc cacagcccct gacaggttta atcacccttc
     2821 tccccccggg cgattccgcc ggtaccggta tcccgtgtga ccggaggatc ttttcggtcc
     2881 tgaccttgtt ggcacaaagg gcaaccgtca tgaagccgca cccaagatta tgcgtgtgct
     2941 gctcaaggat ctgcgtatac cccgccagca gatggtccgg ggcaatcaca agacccatcg
     3001 agcaggtggg ggcaaggcgt tctatctcca caccaaattc ccctgcgccg ggcagcacga
     3061 cttcgtatcc ccggcgctca aaactctgtt tcagcacatc gagcatcgca gcaccctctg
     3121 cggcgagagt tggatcatgg gctgatgcgt attcggcgag cagaactttc atcctttatc
     3181 tctatgcgct gaaagaaaat taccctatcc gatcggtgaa ctatttgaca gtgcagatca
     3241 tacgtgaatg cacatgaaac cctcgatcct gctgactaat gatgatggcg tgagttcagt
     3301 cgggctctgg gctgcatatg aagctcttgc ccctgttgct gatgtaaccg ttgtggcacc
     3361 ggcaacccag cagagtgccg tgggcaggtc gatatcgatc ttcgaacccc tccgggccag
     3421 ccaaatcacg ataaatggca tccctgcctg gtcagttgcc ggaaaaccca ccgatgctgt
     3481 cattattggt ctctatgccc tcaaactgaa cccggatctg atcgtcagcg ggattaatat
     3541 cggggagaac ctctcctttg aatctatcat gacatcgggt acggttggtg ccgcgctcga
     3601 aggctccaac caggggacaa aaggtatcgc gttctcgctc caggtcgagg accagggaga
     3661 taaattcgat gatccgcgtc ataacggaca ggacttcgag gccgctaaaa atgttgtccg
     3721 cgacgtcgta tcccgcatac tggatcataa attctgtccc cacgcagatg taatcaatgt
     3781 caatattccc tcggtagtaa aagggggcta tgagatcacc cgccttgccc gtaagttgtt
     3841 cctgaccggg gttgagaagc gggttgaccc gcggggaaga ccatatttct ggattaatgg
     3901 tccgcttctt gaggatgctg aagaaggaac cgatgtgcat gccatccgta aaggcaatat
     3961 ctccattacc ccgatcacgc tcgactgtac ggcatatgcg gcagaaaaag aggcacggga
     4021 tattttttac aacgattaaa ggacttcatc cgggcataca ggcacagcag ttaataatta
     4081 ctcctcccgc atcagttcct gacgcacggt catccccgct gtagctgaac gataattccc
     4141 agacccgtta tcaccagtat taactattaa aaaccgaaat ctatctgtca gatggacaat
     4201 tcagcagagg cactcatcgc agcaaaacgt gcagcagggt tcgaggctgc agagatggta
     4261 aaagacggta tggtcctggg tcttggaacc gggtccacgg ttttttttgc gcttgaacgg
     4321 ttatcctgcc ggatcaaaga agggctgcag gtttccggtg ttccgacctc cttccagaca
     4381 gcacgactgt cacgggagta tggcatacca ctcaccactc ttgatgagga ccctgtgctg
     4441 gatctcgcgg tcgatggagc agaccaggtt gacaagcatc ttttccttat caaaggcaga
     4501 ggagcagcac tgacacggga gaaatgcgtt gcttccgcgg cgctcagttt tttggttatt
     4561 atcgatgaac agaagttcgt ttcttcactt acagcccctg ttccggtgga agtgatcccc
     4621 tttgcggcca gaccagtcat gaatcagctt gaagatctgg gttgcacggc aatattgcgg
     4681 gagggaatta aaaaggatgg accggtcatt acggacaatg gcaactttgt agttgactgc
     4741 agtttttctg aaattgatga tcctgaagag cttgaaaaaa caataaaaat gctccacgga
     4801 gttgttgaca gtggattatt ctgcggtttt accggtaaga ctaccgtgat agtcggaaat
     4861 caaaaaaggt gccgggtgat taattcaccg gatataatcg cgtaacttct gaacaagggc
     4921 gagctgggta gtcgcttcct tcttcttttc tttttctata ctgtccatga tctcgtgaat
     4981 gggttttgcc agcacgtaaa tcttgaccgg gcgtcccttg ctctctgctt tgctctcgcg
     5041 gctggtaatc cagctctgtt cgatcaggta tcgcatcgca atactgacct caggctggcg
     5101 gagatccgtt ccccgctcaa tcgcccttga tgtagcttca ggagtgtttg ccagaaatac
     5161 caggactttt gcaacgtttc tcttggtgcc gatctcgatg aggagattgg caaattcctc
     5221 ttccttttca gtaaaataca taacgctttc tgttctcatg catcacgtcc gatatatctc
     5281 tctttattta ccaaattaat tatctaatcc ttgttatata tgccatctta ttgttgggcg
     5341 attgacaaaa catatatata ttacgtttaa ggtatccacg tgaaataata aaatataaaa
     5401 aaattaaacc gattttatgg gttttaagtt gtattaaaaa ataatcagat caaaccaaga
     5461 ctgcggagat cggacaggat cttaaccacc gcttttttcg cgtcctctgg ttccttgcct
     5521 ccggtaataa tgagttttcc ggacccaaag agcagtacta caacctttgg tgtgtcaagg
     5581 cgatatacaa gtccggggaa ctgttcgggc tcatattcaa tcttatccag gttgaagcca
     5641 actgcaatct tattcaggtt gataggagtt gccagatcgg cagacgtcac gatattctgg
     5701 atcttgtaat caagcttttt tgggatcgcg atgccaagct tgcggaggag tcctccgaga
     5761 atttccaggc ctttactaag actgtcaata ctctttgcac cggtaagaac aacttttccc
     5821 gaaccaaaaa cgagcgcggc gatcttggga tcctgcatac gaagaacgac accggggaat
     5881 cttttcttgt tatattcggc tccgggaatc ttggacgcga tctcgggtag atccagataa
     5941 tcggtcacct ttgcggaggc aacgatgttt tcaattttga gggagtcctc tggtctgacc
     6001 ttcatattta taaatgtcac aaaacagtat ttaaaaaggt tggttatatt tttcaggctg
     6061 gcatagttta attccccgat ctgtcaggat gaatacaaac caggattttt tccagggtta
     6121 tacagaacag gtcttttccc ttcggatttt cagaagttta gcaaaataat cctggattag
     6181 gaaaagggaa tgatcgtacg gttatatctg aaaccgacac gagtcattat caaaagcacg
     6241 tcaaaaggtg ccggacaatt ttgtagtgtc cgctgcctga tcgattttct cgcacgaatt
     6301 ttttcggata gcatggttct ttcataccgt acgagcgcgc ttttttttgg tgcatcgggc
     6361 gcttttcctt cggtaaaacg taccgcacga attttttaaa gataaccagg aaaaaaagca
     6421 gacccgttct ttttgtctag cctgacgaga aaattgtata tttttctgga ttattggaac
     6481 atggtatata tcaataattt ttagttaatc gtattgtctc caggtatgct cgcacttggt
     6541 gcaccggaag aacctgacct cgctctcatc ggcagcacgg agctggcgga gccaccagat
     6601 agccaggttg tttgcgcact tgggacactt gatctggatg gttggcatcg tcttcatttt
     6661 ctcttcttca tcatcgacaa tccagatctc ccgttccttc ccgattactt catgtttcat
     6721 attgtctgca tcttcaattt tccgtatata accacatttc cggcatttca gctgaccccc
     6781 ggaagatatt aacagacttt tacactcagg acagaacatg ggtatagcat gtgcaagcaa
     6841 aggagattaa ccctcttgtc ggggatatca gcagtgattt tttataaatt acccgtttga
     6901 cagtcaatgc cggaaagctg atttctttgc cattcccctt gtgccttcag aatccttttt
     6961 ttggtatcgg gtggatatgt ctataatgat cgagtatctg gtgctgatat cctgtgttgg
     7021 atttcttgcc ttcctgatcc ccggtcgcca tcagaaatac gcagccatta tcggctggac
     7081 gttcattgtc ctgttcctgt ttgcagaact gccctactat ttctcactga ataatttctt
     7141 atatcccatc atggccgccc tgtcgctgcc attcctgatc ataacctcac gctatctgct
     7201 ggtcgacgat gatcgcgtaa tgcacttatc gcgtgcggca gctgttgcat tcctcatcta
     7261 tgctcccttt gaatacctcc ccctgctcgg cgactggctt ataggaattg tcgtgggaca
     7321 ggtagtctgg atgctcgacg cactcacgta tacggtgagt ctggcggact ggaatatcat
     7381 ttcaagaaac acgttcaggg ttgaaattat ccttgcgtgc accggcatcc agagtattgc
     7441 aataatgctg ggagttgctg ccgcagtacc aacaacaacc cgccagaaaa tctatgcatt
     7501 tgtcatcatc gctcctacca tctatatcct gaaccttttc cggaacgtct ttgtcataat
     7561 tgcctacacc gaacaatggt ttccatatta tccggctatt gccagcaacg gcgaattcgg
     7621 gtacgagagt tttttctggg cgcacaatgt aattgctgaa cttcttgccc tcgtatgcct
     7681 cgtcattatt gcctatggcc tttttaccct tattcccaac ctgggaaaat ttgctgactc
     7741 gctctacaag gcatattcca gcgaaatcag gaaagcgttc ggtaaagata gataattctc
     7801 tgtatagctg aaaggttcgt gcataccgca ataataatca ctgcaacccc gatgaacccc
     7861 gaaatacccc cgagaaataa aaaaaggaag gtctcaggcc tgccgaaaaa tccaactcct
     7921 tcaaggggat catcaatttt accttcaacc ttatcccggt aaccgatttc tgcgtacact
     7981 acgggtttta tgaacgtatt catcagggat ccaataatcg ccagtgcaac gatggcaaaa
     8041 tccgcaaaag aggggaccgt gatatactgg ctcacaatcg aaataccgga aagtccgata
     8101 cctaagagca cgagcccgtc aacgtattta tcgacgatcc agtcaaaaac tgctccgaaa
     8161 ttcgagtgtg aatttgtttt tctggcaaca ctcccgtcga caagatcaaa tacagcagag
     8221 agaataagcg caagggtgcc ccagatgaac tgctgccgga agaagagata cgcacagaca
     8281 ataccggcaa gaagagagag caccgaaatc tggttggggg tgatccttat tttgacaaaa
     8341 atatctgcaa ccggctcaag ataccggata aatcttgggc gaagtgcagt aatattcata
     8401 cctaatttca tgcacgggta agccattaat atttattgtt ttacgtcagg tgaaacggtg
     8461 ttttcattaa acatgcagcc atgcagatat ttgttttttt ttaaaggtct gaagtgttgc
     8521 catcgtcaac ttctttaaat cccccaccca atctaaccgt aattaaggag ttgtaatgac
     8581 taaaacgaca gaccgtcctc atgtgctgat gatgtcggag atcactgcgg acggaaaact
     8641 cacgcttaag aaaggagcgt cgtccaaaat tcttatgaaa tatatgtcgc atgagaccga
     8701 gatcctgctt catacgacga gggcggaata tgatgcgatc atggtcggcg ccaacacgat
     8761 ccgtatcgat aactcctttc tgacggtaag acttgtaccg ggtaaaagcc cgttgcgggt
     8821 tatcccgtgc agcatggcag accttccgct tgatgcgaat gtgctcggaa aggacgcccc
     8881 gactgcgatt gccgtgtgcg aagcagcccc ggaggacagg gttgaggcta tcatgcgcaa
     8941 aggagctcat gttatcgttg ccggaaaaaa ccatgtcgac ctgccccttc tcttatccat
     9001 actgaaaaaa aagttcggca tacaaaaact gatgattgag ggggggccca cgctcaactg
     9061 gcacatgctc cacgacaaac ttgtcgacga gatacgcctc atccacctgc catttattgt
     9121 cgggggtgcc gacacaccat ccctggtcgg gggaatgcac ataaattcag aagacgagat
     9181 gatccggctg aacctcaaga aatactatat gtgcggaagc aatctcgtgt cagaattcga
     9241 tatcctctat catggggcat gaccatgaac ggtatcacac cacctccggt cgggaaccac
     9301 ccgcaggcgc atcccggaat aaaatggttt tttattataa tatttggtat aatttttgtt
     9361 tttggtctgc tcatcgcggt atattttttt gccggcaatg accagatggg agtcacggtt
     9421 ataaggatgg aaggaaccat ggtaaccggc caggtttccg atgcagaaac tatcggaagt
     9481 gaagtagtgg gcaatgagct tcgtgatgct gcagacgacc ctatggtcga agcaattgtt
     9541 ctccgggtga acagccccgg gggcactcct gcagcagcac aggaaattat ccgcgacctt
     9601 gattatgcaa agtcaaaaaa acctgtcatc gtatccatgg gggatattgg aacatcagca
     9661 gcttattacg tcagcgccca cgcggacagg atttatgcaa accccgatac attcacagca
     9721 ggaatcgggg ttatctggaa attttccgat atcagccgct ggatggaaaa ggaaggatat
     9781 aatatcagcg tcgttaaatc gggcacaaaa aaagacatgg gaacaacttc acgtccgatc
     9841 tccgacgaag agcagatcta tgcccagcag attgtaaacg agagttttga aaatttcatc
     9901 agtgatgtca cgtcccagcg accgatcgca cggtccgata ttgaggatgg cagggttatt
     9961 cgtggcacgg atgccgtgcg actgcatatc gttgatgaga tcggtaacc
//
LOCUS       DASRLV010000210        13432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1044267,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000210 DASRLV010000000
VERSION     DASRLV010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 13432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13432
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044267"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            547..3237
                     /locus_tag="VFC43_08080"
     CDS             547..3237
                     /locus_tag="VFC43_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEMAR_RS02690 family S-layer glycoprotein"
                     /protein_id="HZK31170.1"
                     /translation="MTKRLTIALVALAMFVLVAAMPVSADLFNGYYRVAPTINAGATV
                     FIGEQGLDITGALSAHPGVNTIGWWASAADIQATSPTVSFDLTNRATQFTVTQAEFDG
                     YEGEWYQVNPATSHAFVPSAFNVKAPKLDVSIRDPAQNDGADVSGKSIPQGSQIQFQI
                     GTNMYTVLSDVTKRSPVYNNAGSGPNSDGYIDLVVKTESGTVLQKLYDLNNTQQSIVA
                     LNVTTQPYTWGRANAGRGPGIDYVWNTAALTPTGEKAYPAGTYVVSVQSKLNNMKDNY
                     LSGAAAYTGRTVSETKTITLVSDTVSITANKDSVVRTKPFSVTITGKPSAFYNVWVKN
                     TVNMNINDYDNAPPVVTKFQEQVVDGSNITYVYQYQNGGGTKTVGDIAFGQESVIFND
                     PNSVYSYARIKLSTSGSRTVEFSTTHNTKAQKYTIRVETNNGPSYALGDFKGDEVDVK
                     VEKGAVTVVAAGDQSYYLGEEIKLSGTNTESSTTYLFVIGPNLPEKGGVLTEPKTDVT
                     PVPPAAFWNSVKADVLADNTWSYKWGTSNIDLDAGTYTIYAVSQPYDKTPTLLAKAAY
                     GTVSIIIKKPFVSATASQSTVANGDRVYLTGTAEGQPHSVAVWILGKNFAVRAEETVN
                     SDSTFKYEIKQEVTKTLYSGQYFVVVQHPMQNDVFDIDSPGLGVTTGDVYVYNYALGE
                     ATGINDISIFKIQGAGSLQGSDAAEALVQGINDPNVDDTYTKLQFLVEEPIITIDSIG
                     DRHVGDKFTITAKTNLAVDDEILVQVYSSSFKPTQKSQSGEFSGATGTIKVTKGETGM
                     NQLTFDVDSSTFKPDEYIVTEDAVIQQATGTALFNVLEGQAPTTNTTTVVTTFVPTTV
                     VTTVPTTVPPTPTPTKSPGYGALIALIGLGAVAFIVVRRH"
     gene            3438..4961
                     /locus_tag="VFC43_08085"
     CDS             3438..4961
                     /locus_tag="VFC43_08085"
                     /EC_number="2.4.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP phosphorylase"
                     /protein_id="HZK31171.1"
                     /translation="MKLSVKNLDIASRGVLLNRVDARSIGVLDGDRVQVINSKNGVSV
                     AAFVETTSTIAQQGTIGVYRFTNERLHLEEGTEIEVREAGRPASLDFIKKKMDGGKLT
                     KEETLTIIKDVVSDDISGAELTAFIIASYINPLDMDEVEYLTRAMVETGEQIKFASRP
                     IVDKHSIGGVPGNKISLLVVPIIAASGLKIPKTSSRAITGAGGTADLMEVLASVEFSA
                     AEVQKMTEKVGGVIVWGGATNIAPADDRIILQEYPFKIDARGQMLASVMAKKFAVGAN
                     LVVIDIPIGQHTKVATMQEGRKLAREFIELGDRLNMKVECALTYGDIPVGRSIGPKLE
                     VIEALRVLEGATEPNSFIQKSLSLAGIALEMSGKAARGTGFAIAQDILTKGKALEKFR
                     QIIEIQGGDPNVKSTDIIPGEHQFVVNSPSSGYVIEMNNRSLITLARIAGAPHDPGAG
                     ILLHAKKGKLIKAGEPLFTLFAERNWRLQQAIEEGRRLMPILVEGMLLDRVPSISEL"
     gene            5001..6392
                     /locus_tag="VFC43_08090"
     CDS             5001..6392
                     /locus_tag="VFC43_08090"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HZK31172.1"
                     /translation="MYRQRNRFLFTLLCSILLCSLVQATVLQITVQDSIDNSTIPRAT
                     VFLNGENYARTNNNGQVFLNHSGLDDPRILVSLTGYDDWEKQVDKNATSILVNLSRKS
                     IRLNVNIYDSVTLGSISGVRVNISSDRISQTNLTNISGSVSFLVNATTTYSLDITAPN
                     YLTRSAIIEMGTTDKAAQYLMLQANQFFILVNDRNSKTAVPDAEVRIDGTLIGKTDMR
                     GVLAVPLTRGKVYSIEIKKGSYQPYMETRDISQSDAIYAIGLSKALVGAFVSVFDETR
                     SPISGADVFINGTLAGTTNQYGRLNFPNLVSGNYSVEVKKIGYVTLNRTILVANPNEE
                     YSFELSFENADLTLFVQDKDQKMVPNATIILNGQVLGATDDHGQYKAKVKFNIPYNIT
                     ASKDSYQPVAVQKQFAQGNTTFSVTLIMEKSLDWGLIAIIVIGAISVLVLFAALRIWG
                     GGKRRNVMRRNDI"
     gene            6511..9513
                     /locus_tag="VFC43_08095"
     CDS             6511..9513
                     /locus_tag="VFC43_08095"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZK31173.1"
                     /translation="MRSKVGILAIICILLMVGTVAGHLPDTSTITTSNSWIVANGVDQ
                     TTITIMVSNSSLGSMLGATVVFTVDDPKYGSMSPGTAISDATGKATNTFKTNTASGTA
                     VITARIISHDGYNVIKTINQNIDHDGPYYAYFTHPPNGTVATDVPFAISLKDYWKNPI
                     DDRKQIIPGDIHTISLHVHGPAPDDCGFVTANITSHDILSTLDMNGNASLTIRLTKKA
                     GPNNILMNAYGSISDKLDWIDADTNGIPFSISQAYDPSGFPPSLPADGTSEFTIVYSL
                     LDRYDNPTKNAWIWFNTSIPGEEIKLGPSNSGGMITRSYGKKPTIGVVNISAIAISNT
                     SVTIFQVVEFTSTAATNMDITANPETMASRDVNPTIVSDISASVTDIMGNPVENETVT
                     FTMSNASYSGNFNITSSPTLVSTTATSDVDGLATVTFIPGSFTTNSSDPKYSSSATGN
                     CTITAHWGTLHKPILITWKNYPYLSVKTSVNPQTVQVNQTVDVTIAFKGDGWALQPKP
                     IDVVLCTDRSGSMLFDNPDRMFSIREAAKSFVDNMSPARDTLGLVTFGRSGSIIRPGY
                     NSGIAMSEINNVYSPYSWTYSDYATVDRTLIGGISGFNSVKNNISSILPDHGTPMRSA
                     IYKSVNEIKSRGRAGSVKAIILLSDGDYNWYGDPLARGSGSTNGETTFGDLTTSYKTF
                     TGLGAGQFSNQNMSVYATNNGIRIYSIAFSSSVSSGGKFTLERLANATGGKYYEASAT
                     DVSDVYSQIAGDLKTDAGVNTNMITDFQNVNVTGVSLAGAQVYDYVPNSTASTRILWQ
                     DGVTNVTNQSADWLSDNKLDFTIGTIKVGETWEATFRLKVKKSGSIDVFGANSLVSFN
                     NGTSTLTLPHTFLTVVPDLNATGMGSKSLILSNLFVTESGEIKALLPVMWNTSYSGNK
                     TITEKVYYSIDDGPWVMFTTKTIPGYATAPILKEYVDYAQLDVTKLPPGGYKIKVYAT
                     VDDAPDAWIGPTDTKTVGGQGKIYLRLE"
     gene            complement(9531..9602)
                     /locus_tag="VFC43_08100"
     tRNA            complement(9531..9602)
                     /locus_tag="VFC43_08100"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9567..9569),aa:Arg,seq:tcg)
     gene            9659..10096
                     /locus_tag="VFC43_08105"
     CDS             9659..10096
                     /locus_tag="VFC43_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HZK31174.1"
                     /translation="MILSGPEIIRRLDKDCSQERLVITPYSRDCQQPASYDLRSASDI
                     LLPKGTCTLVPTVEWVELPHDLAGTLRCRSSFGRRGIILGAGFVDPGFRGQLTLCLTN
                     MGCDDLIVQKNDRIVQMILHEVREGNLGYSGRYQDSRGAVEAR"
     gene            10099..11049
                     /locus_tag="VFC43_08110"
     CDS             10099..11049
                     /locus_tag="VFC43_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HZK31175.1"
                     /translation="MIRTERKYIEILRILKEHQDPIGAKRLSELMAERGFVLSDRAVQ
                     YYLSYLDTMGFTEKIGNQGRILTRAGLDETDNALVDDRIGFVISKLERLAYRSTFDPS
                     TSTGDVAYNLSIVPLESFEKVKSAFDDVINAGCGFFNSYRIIDRDPRVPTGSVGFITI
                     CSISMDGVFQRNGIPVKMAYGGRLEIDQGIPKQFKDLIGYRGTTIDPLELFISSGLTS
                     ISEYTRTNSGIALANVREVPCSAKNEVEKIIRLMNGCGFVFPVTMGNQVFNLPANPYR
                     LSIVAFSGLNYIGNTVEHGIEITTEIGAGNIQFSKVMEIN"
     gene            complement(11050..13404)
                     /locus_tag="VFC43_08115"
     CDS             complement(11050..13404)
                     /locus_tag="VFC43_08115"
                     /inference="COORDINATES: protein motif:HMM:NF044377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tubulin/FtsZ family protein"
                     /protein_id="HZK31176.1"
                     /translation="MRILAIGLGGAGCRIVYSLYTTDRRSSKVVCVQALAIDVNSDTF
                     AQMGDLPDSAKLFFAPIDPDIQTEDNRDTQTATIDIGEIVARFQNIEIGETDAIFVCC
                     GLGGSMVDAAPHIISALRASITEPIFGLVTLPCLAEGERHSAKAADDLDMLTPLLDGI
                     ILFDNETWYKKTKAMRSTLSKKEKSLAEKFGFRKSEPEISPQLATYLLLNEGIVRRIS
                     LILRAGEFKADGGLDFAEVVLDSGEVLNTMKGMGYITIGYAVERLPHNPLGFLTSLRP
                     TGFFADEHNKKASRIVDLAKQAIYHEISTPCDMTSAHKALVLIAGPSHELSLKGFMTV
                     RKWIDRSIAGLETRSGDYPVMNTKNVAIIIMLSGLENIPRITELKDIQNQYRIKLQDS
                     GTIEKQTESGVITLSENTGRSITDKILTRDGNAGIKEEILIFASETRKNDKTLMQESD
                     DFSRQGNNYSQSFEEGDQKTYSVPTDARKKALNQDAAQPHLPTDAAYQINAEDAGDLN
                     LQRSSATRHKVTAAKGEIKISPSASLSGSPISFSLRKPHIKKSSFGTDHKGLDEKHLP
                     HTMDERLKSKEIERQIIEKELQRQRTMAMSGRTSKTGPETTEKTSHRREVIRLKRDVP
                     KAKSPKRDDEQHDEQSDLAEEIHPEPRKRRIIIQKKTVQSVRMDTSVNEQENRDPVID
                     DDELSSERSTGDYDARTDAEEGRVGLKNPSYKSKDHIFEGKGIQKTASPQVKDSVLIH
                     TELKAKKSIRETKNVENIPEDSDGVKDQSTKQEKKTAKKDINWI"
BASE COUNT         3740 a         3055 c         3050 g         3587 t
ORIGIN      
        1 atgctaggcg cccggataat tctcatggaa acccgtgtgt cattgtacat tgtatatgtg
       61 ttgaaatgtt gtttaatcga aattgaatcg gatgaaccca aagatgaaat tagatatatt
      121 gaaaactgaa aaaggaatta acatttaact gacgaaacta ttaggctaaa tgaacactaa
      181 atctgtcata ataaaaatta tacaattcaa taagaaatta agtaagtaga agtaaagaaa
      241 acactataaa taaatctaat agattattat aatacagtat atttatatct gtagcatttg
      301 gggtagtcgg ggatgttgga taatgagcct aaaatccatt ttaatcgctc aaaacaataa
      361 acagagtcca aatattgccc agatttacct aaaaaaccga ccacaaagga tatattaggt
      421 cttttacaaa tactcttcca tattccgtct aaacggagta tggggctcgt agaactgtta
      481 ttcggcattt gtcgaaatcg ttcacggctt gaaatgtatg atgtaaactt tggaggaatt
      541 gaaagaatga ctaagcgatt aactattgca ctcgttgcac tcgcgatgtt cgtgctcgtt
      601 gcagccatgc cggtatcggc tgacctgttc aacggctact atagagtagc accgaccatc
      661 aatgcgggcg caacagtgtt tattggagaa caggggttag atatcactgg cgccctgtcc
      721 gctcatccag gcgttaatac gattggatgg tgggcatcag ccgcagatat ccaagccaca
      781 tcaccaacag tatcctttga ccttaccaac cgggcgaccc agtttacggt aacccaggcg
      841 gaatttgatg gatatgaagg tgaatggtat caggtaaacc ccgctaccag ccatgcattt
      901 gttccatcgg cattcaacgt taaggcaccc aagcttgatg taagcataag ggaccccgca
      961 cagaatgacg gtgcagatgt ttccggcaaa tcgattccgc agggatcgca aatccagttc
     1021 cagattggaa ccaacatgta cacggttctt tctgatgtaa ctaagcgttc cccggtatac
     1081 aacaatgccg gcagtggtcc aaacagtgac ggttacattg atcttgtagt caagaccgag
     1141 tccggaaccg ttctgcagaa gctgtatgat ctcaataaca cccagcagag cattgttgcc
     1201 ctgaacgtta ccactcagcc ctatacctgg ggcagagcga acgcaggaag aggacccggg
     1261 attgactacg tctggaacac agcagctctt acacccaccg gtgagaaagc ttaccccgca
     1321 ggtacctatg tcgtcagtgt tcagtccaaa ctgaacaaca tgaaggacaa ctacctgagc
     1381 ggcgcagcag catacaccgg aagaacagtt tccgagacaa agacaattac ccttgtctct
     1441 gacacagtga gcatcactgc aaacaaggac tctgttgtcc gcaccaagcc gttctccgta
     1501 acaattaccg gcaaaccaag tgcattctac aatgtctggg tcaagaacac cgttaatatg
     1561 aacattaacg actatgacaa tgcaccaccg gttgttacca agttccagga acaggttgtt
     1621 gacggtagca acattaccta cgtctaccaa taccagaatg gtggcggaac gaagacggta
     1681 ggagatattg ctttcgggca ggagtcagtt atcttcaatg accccaacag tgtctacagc
     1741 tacgcacgta tcaagctctc aacctctggt agtcgaactg ttgaattcag tactacccac
     1801 aacaccaagg cacagaagta caccatccgt gttgaaacaa acaatggacc atcctatgca
     1861 cttggtgact tcaagggcga cgaggttgat gtaaaggtcg aaaagggcgc agtcactgtc
     1921 gtggcagccg gcgaccagag ctactacctt ggcgaagaga tcaagctctc cggtaccaac
     1981 accgaaagct cgaccacgta cctgttcgtc atcggaccca acctcccgga gaagggcggc
     2041 gttctgacag aaccaaagac cgatgttaca ccggtacccc cggcagcatt ctggaactcc
     2101 gtaaaggcgg atgtccttgc tgacaacacc tggtcgtaca agtggggaac ctcaaacatc
     2161 gacctcgatg caggaaccta cacgatctac gccgtaagcc agccttatga caagacacca
     2221 acattactcg caaaagcagc atacggcacg gtttccatca tcatcaagaa gcccttcgtc
     2281 tctgcaactg catcccagtc aactgttgca aatggtgaca gagtctacct gacaggtacc
     2341 gcagagggtc agccccactc ggttgcagtc tggatactcg gtaagaactt tgcagtccgt
     2401 gctgaggaga ccgtaaactc tgactccacg ttcaagtacg agatcaagca ggaagtaacc
     2461 aagactctct acagcggaca gtacttcgtt gtcgtccagc acccgatgca gaacgacgtg
     2521 ttcgatatcg actctccagg tcttggcgtt acaaccggtg acgtgtacgt ctacaactac
     2581 gcacttggcg aagcaactgg tatcaacgat atcagcatct tcaagatcca gggagccggc
     2641 agtctgcagg gatcagacgc agctgaagca cttgtccagg gaatcaacga cccgaacgtc
     2701 gacgatacct acaccaagct ccagttcctg gtagaggaac ccatcatcac catcgactcg
     2761 attggcgaca ggcatgttgg tgacaagttc accatcaccg caaagaccaa cctcgcagtc
     2821 gacgatgaaa tcctcgttca ggtctactca tcatcattca agccgactca gaagagccag
     2881 agcggcgagt tcagcggcgc aaccggtacc atcaaagtca ccaagggcga aactggcatg
     2941 aaccagctta cctttgatgt cgactcatcc acattcaaac cggatgagta cattgttacc
     3001 gaagacgcag tcatccagca ggccaccggc actgcactgt tcaatgttct tgaagggcaa
     3061 gccccgacca caaacacgac aaccgttgta acaacatttg tcccgaccac agtcgtaaca
     3121 accgttccga caacagtgcc cccgaccccg accccgacca agtcgcccgg gtacggtgca
     3181 ttgattgcat taatcggcct cggtgcagtt gcattcattg tcgtgcgcag gcactgaact
     3241 atcatctaaa atctcttttt tttcgcataa cttatcagta agtaaacaaa attatgctgt
     3301 ttttagtcct gtggaattct acaggggtct gatggtctga tttccatagt ggcactcttt
     3361 taatgtccga acggacaacg tagatctgaa aaggattcat tcagtggggt gcatccccgc
     3421 taatggagga ttaggcattg aaattaagtg taaaaaatct ggatattgca tcacgaggtg
     3481 tgctgctcaa ccgggttgac gcccgcagca ttggtgtcct cgatggcgat cgtgtccaag
     3541 ttattaattc gaaaaacggt gtatctgttg ctgctttcgt cgagaccact tcaacgatag
     3601 ctcagcaggg aacgatcggt gtctaccgtt tcacgaacga acgccttcat cttgaagaag
     3661 gcactgaaat tgaagtcagg gaagcaggca gaccggcatc gcttgacttt ataaaaaaga
     3721 aaatggatgg tggcaaactc accaaagagg agacgctgac cataataaaa gatgtggtca
     3781 gtgatgatat ctccggggca gaactcacgg cattcatcat cgcatcttat atcaaccccc
     3841 ttgacatgga tgaagtggaa tacctgaccc gtgctatggt cgagactggt gaacagataa
     3901 agttcgcatc aaggcctata gtagacaaac attcgatagg aggagtgccc ggcaacaaaa
     3961 tctctctcct ggttgtccca ataattgctg caagcggact taagataccc aagacaagtt
     4021 cgcgtgccat aaccggagcc ggaggtactg ccgatcttat ggaagtcctt gcaagtgtcg
     4081 agttctccgc tgctgaggtc cagaagatga ctgaaaaggt aggaggagtc attgtgtggg
     4141 gtggtgcgac caatattgct cctgctgatg accgcatcat actccaggaa tatccgttca
     4201 agattgatgc acggggtcag atgcttgcaa gtgttatggc aaaaaaattt gcggtggggg
     4261 cgaatctggt tgtaatcgat attccgatcg gacagcatac caaagtagca accatgcagg
     4321 aaggcagaaa acttgcccgg gaattcatcg agctgggcga ccggcttaac atgaaagtgg
     4381 agtgtgcact gacctatgga gatattccgg ttggacggag cataggtcca aaactcgaag
     4441 taatagaagc tctccgggtt cttgagggtg caactgagcc gaactcattc attcagaaaa
     4501 gtctttcact tgcaggtatt gcccttgaga tgtccgggaa agctgcacga ggaacaggtt
     4561 ttgcgatagc gcaggatata ctcacaaaag gtaaggctct tgagaagttt cgccagatca
     4621 ttgaaatcca gggtggtgat ccgaatgtca aatcaacgga tattatcccg ggtgaacatc
     4681 aatttgtcgt taattctccc tcatccggat atgtgattga aatgaataac cggtcgctga
     4741 ttacccttgc ccgcatagcg ggagcacctc acgatcccgg agctggtatc cttctgcatg
     4801 cgaaaaaagg taagctcata aaagcgggag aacccctttt tacacttttt gcagaaagaa
     4861 actggcggct ccagcaggca atagaggagg ggaggcggct tatgcccatt cttgtagaag
     4921 gcatgctttt agaccgggtg ccttctatct cggaattgta gaccgatgtg ctcatatatg
     4981 agcaggaggc gatatttctt atgtatcgcc agagaaatcg tttccttttt accctgctgt
     5041 gcagcattct cctctgcagc ttagttcagg caacggtcct gcaaattacc gtacaggata
     5101 gtatagataa ttcaacaatc cctcgtgcca cggtatttct caacggagag aattacgcaa
     5161 gaactaataa caacgggcag gtctttctca atcattccgg attagatgat ccacgtatcc
     5221 ttgtttccct gaccgggtat gacgactggg aaaaacaggt agataaaaac gctacttcaa
     5281 tccttgttaa cctcagtcga aaaagcatca gactgaatgt caacatttac gactctgtta
     5341 ctctgggatc aatatctgga gtgcgagtaa acatttcttc agatcgtata tcgcagacaa
     5401 atctgacaaa tatttccggt tcggtatctt tccttgtgaa tgcaacaact acatattctc
     5461 tcgatattac cgctccaaat tacctgaccc ggagtgctat tatcgaaatg ggcacgaccg
     5521 ataaggctgc ccagtatctg atgttacaag ccaaccagtt ctttattctg gtaaatgaca
     5581 ggaacagcaa gacagccgtt cctgatgcag aagtgcgtat tgacggaaca ctcattggaa
     5641 aaaccgatat gcggggggtt ctggctgtac ccctgacacg tggtaaagtt tattcaattg
     5701 agattaaaaa aggcagctac cagccttata tggaaacaag agacatcagt cagtctgacg
     5761 ccatttatgc catcggactt tcaaaagccc ttgtaggtgc attcgtatcg gtttttgatg
     5821 agaccagaag tcctatttcc ggtgcagacg tatttataaa tggcacactt gccgggacta
     5881 ccaatcagta tggacgtctc aattttccaa atcttgtttc cggcaattat agtgtcgaag
     5941 tcaaaaagat cggatatgta acgctgaatc gtacgatact tgttgcgaat ccaaatgagg
     6001 aatatagctt tgagctgtca tttgaaaatg cagatctgac actctttgtt caggacaaag
     6061 accagaaaat ggttccaaat gctactatca tcctgaatgg gcaagtgttg ggtgcaacag
     6121 atgaccatgg tcagtataag gccaaggtca aattcaacat accctataat ataacggcaa
     6181 gcaaggactc ctatcaacct gtagccgtcc agaaacaatt tgcccaggga aatactacct
     6241 tctcggttac tctcattatg gagaagagtc ttgactgggg acttatcgcc attatagtca
     6301 ttggagcgat aagcgttctt gtgttgtttg ccgctctcag aatatggggt ggaggaaagc
     6361 gcaggaatgt tatgagaaga aacgacatat aattttattt tctggcgttt taaactgtta
     6421 ttttataatt taaacgaaag aaattagttt tttttaaata aatattttta tatacttata
     6481 ctggtaacca tttgatccaa tgtgatttat atgcgttcga aggttggaat tctagcaatc
     6541 atctgtattc tgctcatggt aggaacagtt gccgggcatc tccctgatac ttcaacgatt
     6601 acgaccagta actcgtggat tgttgccaat ggtgttgacc agacaaccat cacaattatg
     6661 gtgtcaaatt cttcactggg ttctatgctc ggagcaacgg tggtcttcac agtggatgat
     6721 ccaaagtatg gctctatgag tcctggaacg gcaatttcag atgctacagg taaagcgacc
     6781 aatactttta aaacaaatac tgcaagcggg acagctgtta taaccgcaag gataatttcg
     6841 catgatggtt ataatgtcat aaaaaccatc aatcagaata ttgaccatga tggtccttac
     6901 tacgcgtatt tcacccaccc tccgaacggg actgttgcaa ctgatgtacc gttcgctatt
     6961 tcgcttaaag attactggaa aaatccgatt gacgaccgaa aacagattat tcccggggat
     7021 attcatacta tcagccttca tgttcatggt cctgctccgg atgattgcgg atttgtgaca
     7081 gcgaatatta cgtctcatga catcctgagt acccttgata tgaacggtaa tgcatcgctc
     7141 actataagat taaccaaaaa agcgggtccc aataatattc tgatgaacgc gtatggtagt
     7201 atatcagata aactcgattg gatcgatgct gatacaaatg gaataccctt ttcaataagc
     7261 caggcgtacg atccctccgg tttcccccct tctcttccgg cagatggaac gagtgaattt
     7321 actattgtgt attccctgtt agaccggtac gacaatccga caaagaatgc ttggatatgg
     7381 ttcaatacat ccattcctgg tgaggaaatt aagctgggac catcaaactc cggggggatg
     7441 ataacaagat cctatggtaa gaaaccgacg attggggtag ttaatatctc tgcgatagca
     7501 atttcaaaca cctcggttac catctttcag gttgtagaat ttaccagcac tgccgcgaca
     7561 aacatggaca taactgcaaa tcctgaaact atggcaagca gggatgtaaa ccccacgatt
     7621 gtatctgata tttctgcatc agtcactgat atcatgggaa atccggttga aaatgaaacg
     7681 gtaacgttca caatgagtaa cgcgagctat tccggcaatt tcaacataac ctcaagcccc
     7741 actttagtga gtactactgc aactagcgat gtggatggac ttgctaccgt aaccttcatt
     7801 cccggaagtt tcacaacaaa cagttccgat ccaaaatata gctcgtccgc aaccggcaat
     7861 tgtacaatta ctgcacactg ggggactctt cacaaaccca tcctgataac atggaaaaac
     7921 tatccttacc tgagtgtgaa aacatcggtc aatccccaga ccgttcaggt aaaccaaact
     7981 gtggatgtga cgattgcctt taaaggtgac ggatgggcac ttcagccaaa accgatcgat
     8041 gtagtattat gtactgacag gtccgggagc atgctctttg ataatcccga tcgtatgttt
     8101 tcaattcggg aagcagcgaa gtcgtttgtg gacaatatga gccctgcgag agacactctg
     8161 ggactggtta cctttgggag gagcgggtct atcatcagac ctggctacaa ctccggaatc
     8221 gctatgtcag aaatcaataa tgtttattcg ccgtattcat ggacttacag tgactatgcc
     8281 acggttgaca ggacgttaat tggggggatt tccggattta attctgtaaa aaataacatc
     8341 agcagtattc ttccggatca tggaactcct atgagatcgg caatttacaa gtcggtcaat
     8401 gagatcaaga gccgcggcag ggccgggtcg gtcaaagcta ttattctcct gtcagacgga
     8461 gattataact ggtatggtga cccgcttgcc cgaggatcag gcagtaccaa tggtgaaaca
     8521 acctttggag acctgactac cagttataag acatttaccg gactgggggc tggtcagttc
     8581 tccaatcaga atatgtcagt ctacgcaacc aataacggta taaggatata ttcgatcgct
     8641 ttctccagca gtgtttcatc tggagggaag tttaccctcg aaaggctggc aaatgccacc
     8701 ggggggaagt attacgaggc atctgctaca gatgtctctg atgtttattc ccagattgcc
     8761 ggtgatctaa agaccgatgc cggtgtgaac accaacatga taacagattt tcagaatgtg
     8821 aatgtcaccg gtgtatcact ggctggggct caggtatatg actatgtgcc gaactcaacc
     8881 gcttcaacaa ggattctgtg gcaggatggt gtaacaaatg ttacaaacca gtctgctgac
     8941 tggctttcag ataataaact tgacttcact attggtacga taaaagttgg tgaaacctgg
     9001 gaggcgacat tccggctgaa agtcaaaaag agcggaagca ttgatgtgtt cggtgcgaat
     9061 tcgcttgttt ctttcaataa tgggacgtca accctgacat tgccgcacac cttccttacg
     9121 gtggttcctg atctcaatgc aactggcatg ggatcgaaat cattaatact ttctaatctc
     9181 tttgtgacgg aatcaggaga aatcaaggca ctcctgccgg taatgtggaa tacgagctat
     9241 tcgggtaaca aaacaattac tgaaaaagtc tactattcca ttgatgatgg gccctgggta
     9301 atgtttacca caaaaacaat tcctggttat gcaacagcac ccatattaaa agagtacgtg
     9361 gattatgctc aacttgacgt aacgaaactc cctccgggag gttacaagat caaggtttat
     9421 gcgacagttg atgatgcacc tgatgcatgg ataggaccaa ctgacactaa aacagtcgga
     9481 ggtcaaggta aaatatacct gagacttgaa taattcaatt tttaataaaa cggacccagc
     9541 gggaatcgaa cccgcgtcct tgggttcgaa gcccaagagg atgtcctcta ccccatggat
     9601 cctgccattc tcttttatat caaaagatat taagtatttt ctaccgatat tgtagtgaat
     9661 gatcttatcc ggccctgaga ttatccgccg cctcgataag gattgttcac aagaacgcct
     9721 tgtgatcacg ccttatagtc gcgattgcca gcaacctgct tcttatgatc tgcgttctgc
     9781 aagcgatatc cttcttccta aaggtacatg tacacttgtt ccgaccgttg aatgggttga
     9841 acttccccat gatctcgccg gaacgctccg ctgcaggtca tccttcggca gacgggggat
     9901 aattctgggt gcaggttttg tagacccggg atttcgtgga caactgacac tctgcctgac
     9961 aaatatggga tgcgatgatc ttattgtaca gaagaatgac cgtatagtgc aaatgatcct
    10021 tcacgaagtg agggagggca acctaggcta ctcaggtcgt tatcaggaca gccggggggc
    10081 cgtggaggca aggtaaccat gattcgtact gaaagaaaat atattgagat tcttcgcatt
    10141 ctgaaagaac atcaggatcc tataggggca aaaaggcttt cggagctgat ggctgaacgt
    10201 ggttttgtcc tcagtgaccg ggcagtccag tactatctca gttatcttga cacgatggga
    10261 ttcacggaaa aaatcggcaa ccagggaagg atattaacaa gggcaggact tgatgaaact
    10321 gataacgctc ttgttgatga tcggattggt tttgtcatct ccaagctcga acgtctcgct
    10381 taccggagta catttgatcc ctcaacgagt actggagatg tggcctataa cctctcgatt
    10441 gttccccttg agtcatttga aaaagtgaag tcagcgttcg atgatgtgat aaatgcaggg
    10501 tgcggtttct tcaattcata ccggataatc gatcgtgatc cccgtgttcc aacgggttcg
    10561 gttggattta taacgatttg cagtatcagt atggatggtg tgtttcaaag aaatggcata
    10621 ccggtaaaaa tggcttatgg aggaaggctg gagatcgatc agggtatacc aaaacagttt
    10681 aaagatctga tcggttatcg gggaacaact attgatccgc tcgaactttt catttcatcc
    10741 ggcctcacgt caatatccga gtatacacgg acaaattccg gaatagcact tgcaaatgtc
    10801 cgggaagttc cctgttcagc aaaaaacgaa gtcgagaaaa taatccgtct tatgaatgga
    10861 tgcggttttg tattccccgt taccatggga aatcaggtgt ttaacctgcc tgcgaatcct
    10921 taccgccttt ccatagttgc attcagtgga ctaaattata taggaaatac cgttgaacat
    10981 ggtattgaga ttacaaccga gattggtgcc ggaaacattc agttttcaaa agtaatggag
    11041 ataaattaat catatccagt taatatcttt ttttgcagtt tttttctcct gtttcgtgga
    11101 ttgatctttt acgccgtctg aatcttccgg gatattctca acattcttag tttcccggat
    11161 actttttttc gcttttagtt ctgtatgaat aagtacagaa tccttaacct gtggagaggc
    11221 tgttttctga ataccttttc cttcaaagat gtggtctttg gatttatagg aaggattttt
    11281 aagaccgact ctaccttctt cagcatcggt tcgtgcatca taatcgccag tactcctttc
    11341 tgaagacaat tcatcatcat ctataacagg atcccggttt tcctgttcat tgacagatgt
    11401 atccatcctt acggattgaa cagtcttttt ttgtatgatt attcttcttt tcctgggctc
    11461 cgggtgaatt tcttcagcca gatctgattg ttcatcatgc tgttcgtcat ctctttttgg
    11521 ggatttcgct tttggaacat cgcgttttaa tctgatcact tcacgacgat gagaagtttt
    11581 ctctgtggtt tccggtcctg tttttgaggt acgaccggac attgccatcg ttctctgtcg
    11641 ttgtaattcc ttctctataa tctgccgttc gatttccttg gatttcaatc gttcgtccat
    11701 tgtgtgcggc agatgctttt catccaatcc tttgtgatct gtaccaaatg aggatttttt
    11761 gatatgtggt tttcttaaag aaaaggatat aggggatccg ctaagtgatg cagaaggtga
    11821 aatttttatt tctcccttgg ctgcagtaac cttatgacgt gttgctgatg aacgctgcag
    11881 gttaaggtca cctgcatctt ctgcgttaat ctggtatgca gcatccgtgg gaaggtgtgg
    11941 ttgtgctgcg tcttgattaa gtgccttctt tctggcatcc gttggaacgg aataggtttt
    12001 ttggtcaccc tcttcgaaag attgggaata attgttaccc tgacgggaaa aatcgtctga
    12061 ttcctgcata agggttttgt catttttccg tgtttcggaa gcaaagataa ggatctcctc
    12121 ttttatcccc gcatttccat cccttgtcaa tatcttgtct gttatactcc tcccagtatt
    12181 ttcagataaa gtaatcacac cagattctgt ttgtttttcg attgtgccac tatcctgaag
    12241 ttttattcta tactggttct ggatatcctt aagttcggtg atgcggggaa tattttcaag
    12301 cccggaaagc atgatgatga ttgcgacgtt cttcgtattc atgacgggat aatccccgga
    12361 tcgggtttcc agcccggcaa tacttctgtc aatccattta cgcactgtca taaatccttt
    12421 caaggaaagc tcatgagacg gaccggcgat caatactaat gccttatgtg cgcttgtcat
    12481 atcgcagggt gtggatatct catgataaat tgcctgtttt gccagatcaa caattcgaga
    12541 ggctttttta ttatgctcat ctgcgaaaaa acctgtcggg cgtaaacttg tcaggaaacc
    12601 taatggatta tgggggagac gttcaaccgc gtatccgatg gtgatgtaac ccatcccttt
    12661 catggtattg agcacctcgc cggaatccag cacaacttcc gcaaaatcaa gtccgccatc
    12721 tgccttgaat tctccggctc tcagaatgag gcttattctc ctgacaattc cctcgttcag
    12781 gagtaaatat gtggcgagtt gtggagaaat ctcgggctca cttttcctga acccaaattt
    12841 ttcagccaaa ctcttctctt tttttgaaag agttgacctc attgccttgg tttttttata
    12901 ccatgtttcg ttatcaaaga gaataatacc gtcaaggagt ggggtcagca tgtccagatc
    12961 atcggccgct tttgccgaat ggcgctctcc ttcagcaaga cagggaagag ttacaagacc
    13021 aaagatcggt tcagttatcg atgcacgaag ggcagaaata atatgtggtg cagcatcaac
    13081 catgctgccc ccaagaccac agcatacaaa gattgcatca gtttccccaa tctcaatatt
    13141 ctgaaagcgg gcaactattt caccaatgtc aattgttgcc gtctgggtat ctctattgtc
    13201 ctctgtctga atatcagggt cgattggcgc gaaaaataat ttcgcactat cgggcagatc
    13261 ccccatttga gcgaatgtat cagaatttac atcaatcgca agtgcctgaa cacacacgac
    13321 tttactgctg cgcctgtcag tagtatataa actatacaca atcctgcagc cggcgccgcc
    13381 caatccaata gcaagtatgc gcatgaacaa atcccaatta aatccctatg gg
//
LOCUS       DASRLV010000211         5924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_239444,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000211 DASRLV010000000
VERSION     DASRLV010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239444"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /gene="grpE"
                     /locus_tag="VFC43_08120"
     CDS             complement(<1..333)
                     /gene="grpE"
                     /locus_tag="VFC43_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HZK31177.1"
                     /translation="MKEDLDQIKKDLDEQTRIAQERLERLKYLQADFDNYRKWSEKEK
                     GSIIALANENLIKDLLVILDDFEQAMPSLGQEKNREGIQMIQKKMQKILKEYGLESIE
                     GVGKKFDPY"
     gene            complement(884..1180)
                     /locus_tag="VFC43_08125"
     CDS             complement(884..1180)
                     /locus_tag="VFC43_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31178.1"
                     /translation="MSKEKPMTTIKQEFIEPVTMLHNDGKFIHIVSKLPGIAEEKIKI
                     DIDLEKTTITIVAADTTKMYKKIISLPGNVIFSKKRFSDGELSLTVEKKGPETV"
     gene            1679..1906
                     /locus_tag="VFC43_08130"
     CDS             1679..1906
                     /locus_tag="VFC43_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31179.1"
                     /translation="MSSSDDIRVKAAKAVDNARVAVNEAVDDAKTAVHNATTPKTTVQ
                     NVVDDVKIEAHKISSDSKIKMHKVEEELKKK"
     gene            2277..2468
                     /locus_tag="VFC43_08135"
     CDS             2277..2468
                     /locus_tag="VFC43_08135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31180.1"
                     /translation="MDGDNGKIIDANTFILDLLGYPLDYFMGKHLWELGFFKDKTLAQ
                     EAFTQLKTNGYIRYEDLPL"
     gene            complement(2484..2639)
                     /locus_tag="VFC43_08140"
     CDS             complement(2484..2639)
                     /locus_tag="VFC43_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31181.1"
                     /translation="MAGDTAEQVEFFICKSQRFFRTFPCSNITDIALDNFLVADKIDV
                     ADKFNRN"
     gene            2646..3026
                     /locus_tag="VFC43_08145"
     CDS             2646..3026
                     /locus_tag="VFC43_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31182.1"
                     /translation="MNKLNVIEAYFEMSRSQSPNSKMQEFIGNEITTAKAIRHLINFA
                     KDYEGMGVNSPVWQDVSSCIQKNNTSLPLGDVKVIVNGPRAEVFADMLFQKVFYNLID
                     NALRYGGNQLKTITVSSEGMTRDL"
     gene            3023..3241
                     /locus_tag="VFC43_08150"
     CDS             3023..3241
                     /locus_tag="VFC43_08150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK31183.1"
                     /translation="MIAVEDNGAGISAADKEHLFKRGFGKQIGLGLFLSREILSITGI
                     TITENSLPGKGARFEISVPKDGYRFIKK"
     gene            complement(3349..4704)
                     /locus_tag="VFC43_08155"
     CDS             complement(3349..4704)
                     /locus_tag="VFC43_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZK31184.1"
                     /translation="MSKVLVVDDEAIITMQLEERLSSMGYTISGMAASGEEAIEKARR
                     LLPDIVLMDIVMPGKMNGIDAAKIITEELDIPVVFVTSYADDAIIEKAKHVRPYGYIV
                     KPFNELEIKAAIEVALFRKATEQQQHKISRRTAQEKVSPGVSESQAQKDDLAYYDLPE
                     IKSVLLKDIFTDIVLYLYTKQVVKEPIFKFALEEGIKKGGRNLFSYTQSVLQKYFVKE
                     IHNKELFTHRIKTNEVFALLPLLEKCTESLQNPGTTGSLQILLDFSETDEFKDILAVR
                     KLLLSKKEEGIPVAGIIAVNMENMDHTMIQSLSEGISKIIVSTGKETTLSFAHHTFPA
                     GSVSVVSQSTIDDVVKKSLEPVVLSLLDRPISGYDIVHEIHNRYNVLIPQARVYTLLY
                     ELLENGYLEIRISGKSKLYCPTEKGKKHFSQKLNDFRQVFRHILSNGRPTKPDSNEGR
                     K"
     gene            complement(4708..>5924)
                     /locus_tag="VFC43_08160"
     CDS             complement(4708..>5924)
                     /locus_tag="VFC43_08160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HZK31185.1"
                     /translation="IRTVLWNSGTIFGSDGKTIIATIAQGQDITDRKKIESEYRHRAA
                     EYAEMNVMLNEEIRHRQSAESNLKKTLSLLNASIESTADGILVVDRQGMITSYNQNFM
                     NMWNIPEGMVTYWDNQIIIRYFQSLLKNPDRFLADMKDLLRHLTRESYDVIELKDGRI
                     FERYSKPQKIGKTTVGRVWSFRDITDRRRSEEKLLASLQEKEVLLREIHHRVKNNLQL
                     VTGLLDMTRMRTRDEATTSILTDMMLKIQTMAQIHTRLYESKQFGKIGLTGQFRDQIV
                     ALSNIYSRKGHEISCEINTQEIFLPVDQALPCALAVNEILSNSYKHAFKGKKEGSIEI
                     SAVQENGEISIRVHDNGIGMPTGFDISRANSLGLKLVRTLVQHQLKGSLTINSQRGTE
                     TIIEFPIAGTEA"
BASE COUNT         1498 a         1339 c         1330 g         1757 t
ORIGIN      
        1 atacggatcg aattttttcc cgacaccctc aatggactct aagccgtatt ctttgaggat
       61 tttctgcatt tttttctgga tcatctgtat cccctcccgg ttcttttcct gtccaaggga
      121 tggcattgcc tgttcaaagt cgtccaggat aaccagcaga tctttgatca ggttctcgtt
      181 tgccagtgca atgatcgatc ctttttcttt ttcagaccat ttgcgataat tgtcaaaatc
      241 tgcctggagg tatttcaacc gttcgagacg ttcctgtgcg atccgtgtct gctcatctag
      301 atcttttttt atctggtcca ggtcttcttt catggctccc gccttctccg gtacatgttc
      361 cggttcatga actgtccttt aaacacaaag aatgacaaaa ccgctttcat cgtctctcct
      421 ttttagggta cgggttctct tggtccatag gtattcatcc tatgtttcac ttgaagttag
      481 tgcttactta atatatagtt tttgatgctg acaatacaga ctacctaatc tccattgggg
      541 catttccagt attgatgaat aaattcataa aaaaaacaat cattgagctc acagtttccg
      601 cacccgttta ccggacaaga tgttccaaaa cgctggaata cggcattacc gtgtttaggg
      661 aaattacccc gcacattccg gtaatttccc cagatacggt ttacttcttc tccggactgg
      721 tatggtacca ggggatccac aaagacagga attatcttca cccgtatcgg gtattgaata
      781 gggtctttta taggatttat cctaaaaaac agtttttgaa gcaaattttg aaacaatatc
      841 tattcctgag aagacatgta gtgccatgta gttcatgcag gcactaaaca gtttcaggac
      901 cctttttttc aacggtaagg gagagttctc catcagaaaa acgctttttg gaaaaaatca
      961 cgttcccggg aaggctgatt atttttttgt acattttagt agtatctgcc gcgacaatag
     1021 ttatcgtggt tttttcaaga tcgatatcaa ttttgatttt ttcttcggct attcctggca
     1081 gttttgaaac aatgtgaatg aattttccgt cattgtgaag cattgtaact ggttcgataa
     1141 attcttgttt tatagtggtc attggttttt ctttggacat gatcacgatt ctctttttct
     1201 catttttggc cagtgcatgg agtattcctt accttccagc tacatccaaa ttccactatc
     1261 tgaaccttgt aaaaaaacta tctgagggta aatggtaccc atcccggtca cggaggaatc
     1321 aggtttattc atggttgcag tgctatcttt tgattgaacc tgatatatgg ggactttata
     1381 tgatacttca gaggctttta tatggtggat tgcaggacct tatccctatt ccccgaagat
     1441 ggaacaattt cccgcatctc cacaaactgg cattgtccag attctggtcc atagtttcaa
     1501 taaggtcatt tctgagtcct gcggtctcaa cgttgacgtt acacctccct tgggtctctt
     1561 tatacccgac tgttacctgt tcatcaagaa acataaacat atctggttgt tacaaatctt
     1621 gattccattt ttataacaat cgggctgcta taaatattcc accggagaat agggcagcat
     1681 gtcatccagt gacgatataa gggtcaaggc cgccaaggca gttgacaatg cccgcgtagc
     1741 agtgaacgaa gcagtcgacg acgcaaagac cgcggttcac aacgcaacaa ctcctaaaac
     1801 gactgtgcag aacgtggtcg atgatgtgaa gatagaagca cataaaatca gttcggattc
     1861 gaagatcaaa atgcataagg tggaagaaga actcaagaaa aagtagccgt aagagtgata
     1921 ctgcttaagg aacgggtgta atggagaatc aggaacgtcc ttttggaatg cctgcctgcc
     1981 tttgcagcag cctcggactt ttttttctca taaaaaacgg agtacgtgaa ttgactactt
     2041 ttccccgatc atggtctgaa ttcagtaccg ggtatcccga aaacaattcc ggcaggcacc
     2101 gttgccagtc ctcctgacct ggtacccctt gaccaggacc gggccggccg gttcccgttc
     2161 atcatgaccg ggaagatgat cgtgaagaaa aatccacacc ttcggaaaat gacctgaagg
     2221 tttcagaact gaagtaccgg aggttatttg agacagcgca ggatgcaatc ctgatcctgg
     2281 acggtgacaa cgggaaaatc attgatgcga ataccttcat tcttgacctt ttaggctacc
     2341 ctctggatta ttttatgggc aaacatctct gggaactcgg atttttcaaa gataaaaccc
     2401 ttgcacagga ggcatttacc cagctgaaaa caaacgggta tatacggtac gaagatctgc
     2461 ccctttaaac aaatacaggg cgttcaattg cggttgaatt tgtcagcaac gtctatcttg
     2521 tcggcgacaa gaaaattatc cagtgcaata tccgtgatat tactgcacgg aaacgtacgg
     2581 aagaagcgct ggctcttgca aataaaaaac tcaacctgct cagcagtatc acccgccacg
     2641 atattgtgaa caagctcaat gtcatcgaag cgtactttga gatgtcccga tcacagagtc
     2701 caaacagcaa aatgcaggaa ttcatcggaa atgaaattac tactgcaaag gcgatccgcc
     2761 atctgataaa tttcgcaaag gactacgagg gtatgggagt aaattcaccg gtctggcagg
     2821 acgtatcatc gtgtattcag aaaaacaata cctccctccc gctcggtgac gtgaaggtca
     2881 tcgtaaacgg gcctcgggca gaggtatttg cagacatgct cttccagaag gtattttata
     2941 acctgatcga caacgcgctc aggtacgggg gaaaccagct gaaaactatc accgtatcat
     3001 ctgaggggat gacgcgggac ttgtgatcgc tgttgaggat aatggtgccg gaatcagcgc
     3061 cgcagataag gaacatttgt ttaaacgggg ttttgggaag cagatcggcc ttggcttgtt
     3121 tctttcccgt gaaatcctgt cgataaccgg aattaccatc accgagaaca gccttcccgg
     3181 gaaaggggcc cggtttgaaa tttccgtgcc aaaggacggg taccggttta taaaaaagtg
     3241 aaaaagcatc cttcgtcagg aatacctgct ttgctgcagc ttgggttacc tctctaaaag
     3301 cagcaaccca ttccatgttc atttaatata caggtttgtc tgggtttttt atttcctgcc
     3361 ctcattgcta tccggctttg tgggtcttcc attactgagt atgtgcctga aaacctgccg
     3421 gaaatcattg agtttctgac tgaagtgctt ttttcctttt tctgtaggac aatagagttt
     3481 ggattttcct gatatcctga tctccaggta gccgttttcc agcagctcgt acaggagcgt
     3541 gtaaaccctt gcctgcggga taaggacatt ataccggtta tggatctcat ggactatgtc
     3601 gtagcctgaa atcggtctgt caagaaggga caggacaaca ggttcaaggg atttttttac
     3661 cacatcatcg atagttgact gggatacaac cgaaacagat cctgcaggaa aggtatggtg
     3721 ggcaaatgag agcgtggttt ctttcccagt tgaaacaatg atcttggaaa ttccctcaga
     3781 cagagactgg atcatggtgt gatccatgtt ctccatattc acggcgataa ttcctgcaac
     3841 cggaatccct tcctcttttt tagaaaggag cagttttctg acagccagga tatctttgaa
     3901 ttcgtcggtc tcagaaaaat ccagaaggat ttgaagagat ccggttgtgc ccgggttctg
     3961 gagagactca gtacatttct ccaggagcgg gagcagtgca aatacttcgt ttgtcttaat
     4021 ccggtgcgtg aaaagctctt tattgtgtat ctcttttaca aagtatttct ggagaactga
     4081 ctgggtatag gaaaacaggt tgcggcctcc tttttttatg ccttcttcta aagcaaattt
     4141 gaatatgggt tcttttacga cctgtttagt atagagatag agaacgatat cagtgaagat
     4201 gtcttttaag agaacggatt ttatttcggg cagatcgtaa tacgccagat cgtctttttg
     4261 tgcctggctt tcggaaacac ccggtgaaac tttttcctgc gcagttcttc tgctgatctt
     4321 gtgctgctgc tgctctgtgg ctttccggaa cagcgcaact tcaatagcgg ctttgatttc
     4381 cagttcattg aagggtttta caatgtagcc ataaggccgg acatgtttag ctttttcaat
     4441 gattgcatcg tctgcatagg aagtgacaaa aacaaccggg atatccagtt cttcagtgat
     4501 tatttttgcc gcgtcgatcc cattcatctt ccccggcata acaatatcca tcagtacgat
     4561 atcgggcagg aggcgccggg ctttttcaat cgcttcttcg ccggaagccg ccattcctga
     4621 aatggtatac cccattgagc ttaaccgctc ttcgagttgc attgttatga tcgcttcatc
     4681 atccacgaca agtacctttg acatagttca agcctccgtt ccggctatgg gaaattcgat
     4741 aatcgtttca gtaccccgct ggctgtttat ggtaagagat cctttcagct ggtgctgtac
     4801 cagggtcctg accagtttaa gcccgagact gtttgcacgg ctgatatcga aaccggtcgg
     4861 catccctata ccattatcat ggaccctgat actgatctcc ccgttttcct gtactgcaga
     4921 gatctcaatt gacccttctt ttttaccttt aaaagcatgt ttatacgagt ttgagaggat
     4981 ctcgtttacc gcaagcgcac acgggagcgc ctggtcaacc gggagaaaaa tttcctgggt
     5041 attgatctca cagcttattt cgtgaccttt cctggaataa atatttgata acgctactat
     5101 ctggtcacgg aactggccgg ttaagccaat tttcccgaac tgtttgcttt cgtacaggcg
     5161 ggtatgaatc tgggccatgg tctggatttt cagcatcata tcggtgagaa tactggttgt
     5221 tgcttcgtca cgggtccgca tgcgggtcat atccagcaga ccggtaacca gctggagatt
     5281 gttcttgaca cggtggtgga tctcacggag cagcacttcc ttttcctgta aggatgcaag
     5341 aagtttttcc tctgagcgac ggcgatcggt tatatcccgg aaactccaga ccctccccac
     5401 cgtggtcttt ccgattttct ggggttttga gtaccgctcg aatattcgcc cgtcttttaa
     5461 ttctatgacg tcatagcttt cccgggtcag gtgccggagg aggtctttca tatcggcgag
     5521 gaaccggtca gggttcttga gcagtgactg gaaatacctg attatgatct ggttatccca
     5581 gtaggttacc ataccctcgg gaatattcca catgttcata aagttctggt tataactggt
     5641 aatcataccc tggcggtcga ctaccaggat accgtcagca gttgattcaa tggacgcatt
     5701 gagcaatgac agggtttttt tgagattcga ttccgctgat tgccgatggc ggatctcttc
     5761 gttaagcatg acattcatct ctgcatattc agcagccctg tgtctgtact ctgattctat
     5821 ctttttccgg tctgtgatat cctgtccctg ggcaattgtt gcaataatgg ttttcccatc
     5881 agatccaaaa atagtgccag agttccagag tacagtcctg atct
//
LOCUS       DASRLV010000212         8075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1136756,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000212 DASRLV010000000
VERSION     DASRLV010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 8075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8075
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136756"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VFC43_08165"
     CDS             complement(<1..594)
                     /locus_tag="VFC43_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HZK31186.1"
                     /translation="MENMFLGRNEAPIGADTWHLLDTMMIEAAKSQLAGRRLLTLEGP
                     FGFGQKILPLGDYETEEGVYTSSFVPLSIIKSGFSLGKRDLAAFEKDKLAFDGGPVAN
                     AAMDCAAKEDQILFNGSGGASGLMNVEGAGSQTLTKWDKIGTAADQIIEAVTKLDEAG
                     FHNGYCMALAPSLYNLLLRRYPQGDGTELDHIRTMVTE"
     gene            complement(596..970)
                     /locus_tag="VFC43_08170"
     CDS             complement(596..970)
                     /locus_tag="VFC43_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HZK31187.1"
                     /translation="MPEFVNPFSGKIPDRTLTTQELIRAIRLDLSAEHEAVHTYMAHA
                     DATDNPVVKAVLIDIANEERVHAGEFARLLAILTGDEDAFLQKGALEVDAFYAKVAPR
                     AEVKQEQDAPKVGSLKPAQKGD"
     gene            complement(991..1608)
                     /locus_tag="VFC43_08175"
     CDS             complement(991..1608)
                     /locus_tag="VFC43_08175"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HZK31188.1"
                     /translation="MEPVKLYSLPKLPYEYTALAPYISEEQLKLHHLKHHQAYVTGAN
                     AILEKLDKARKDNADLDFKAVLKALSFHMGGYRLHNRFWENLAPAGKGGGGIPKGDLA
                     KAIDGEFGKFDRFKKEFTQTATSVEGSGWAVITYCEKTKRPLIMQIEKHNVNIIPGFH
                     ILMVLDVWEHAYYLDYKNDRAKFVESFWNIVNWDMVSKRFDLMTK"
     gene            complement(1989..2570)
                     /locus_tag="VFC43_08180"
     CDS             complement(1989..2570)
                     /locus_tag="VFC43_08180"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HZK31189.1"
                     /translation="MVEGLDISQTRIQELIRDLSHKNEDMRWGAAFALARIGVPAVDL
                     LIPALDDKDSVVRLRAAWALGQIGDQRAVDRLIYTLRDGDWAVRMRAADSLGKIRAQK
                     ATDAILLLLRDENQDVRRHAVAALVHIADPASADRLGDVLKDPDWRVRMGSALALAAI
                     GDQKSFLYLEAATRDKNEFVRKIAESVMKKRQT"
     gene            complement(2606..2854)
                     /locus_tag="VFC43_08185"
     CDS             complement(2606..2854)
                     /locus_tag="VFC43_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HZK31190.1"
                     /translation="MIKKQDGRKEPFVREKIIVSAVKSGADPDYARTIAGEIERNVKE
                     GTTTLEIKRKVLAMLKSQNPEWERNWMVYDTAVKKRSD"
     gene            complement(2955..3434)
                     /locus_tag="VFC43_08190"
     CDS             complement(2955..3434)
                     /locus_tag="VFC43_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HZK31191.1"
                     /translation="MVRWYYKSIFDELDEMRNYMDALYHQMYETNPTALLPSGRESAM
                     KMLPAHHPRLGVDVSVHGDEVVITADLIPGVTKKDITLNLINPQALEISWERKEEKKE
                     EEEGYYLRERRFGSMTRIIPLPLPVTDDKATATVKNGVLEVHLKKTAKTAKGNIPID"
     gene            complement(3722..4243)
                     /locus_tag="VFC43_08195"
     CDS             complement(3722..4243)
                     /locus_tag="VFC43_08195"
                     /inference="COORDINATES: protein motif:HMM:NF039927.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5946 family protein"
                     /protein_id="HZK31192.1"
                     /translation="MTWSRQAVRMTECPGCRVRLPDMHLDPDGRLNASGECWQAFSYL
                     QCYTVSKQDPGFIHQHVVDAYTAQHAGGLTRPIAVAFGLIGLHLALEKGYTGREVQKA
                     HMKIAKIKKDWPFLEPPVRSAVLTVTDVLREETERQKDAMIRKWMEAIWEIWEDHHQW
                     VRATTDELLSKNR"
     gene            complement(4463..5518)
                     /gene="dnaJ"
                     /locus_tag="VFC43_08200"
     CDS             complement(4463..5518)
                     /gene="dnaJ"
                     /locus_tag="VFC43_08200"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HZK31193.1"
                     /translation="MGKKDYYETLGVKRDASQDDLKKAFRQLARKYHPDLNKGSKEAE
                     EKFKEINEAYQVLSDPQKKAEYDQVGHTAFKPGDTAGYKTPSYDDLFRDFGLGDIFDA
                     YSAGSGRVRSRAGADLRYEIEISLSDAYYGTKNSVEVPHSYECSTCHGSGAQPGFSRT
                     CNQCGGSGEIRTIQRSGRQQVMNIAQCPGCGGRGTIIGKPCETCQGRGTIKKTRRIEV
                     SIPAGVEDGQFLRIAGEGEPGENHGPPGDLYAVVHIKKHPVFERQGADLYSTAVVGLA
                     TALLGGEIKVPTINGSATLKIPRGTQSHTLFRLKEQGMPYLNSSGRGDLLMKVIVKIP
                     ENLTKKQEELMKEAFVG"
     gene            complement(5536..7413)
                     /gene="dnaK"
                     /locus_tag="VFC43_08205"
     CDS             complement(5536..7413)
                     /gene="dnaK"
                     /locus_tag="VFC43_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HZK31194.1"
                     /translation="MGKEKIIGIDLGTSNSEAAVMLGGKPTIIPSAEGATVAGKMFPS
                     YVAFTPDGQLLVGEPARRQAISNPEGTVTAAKRKMGTDQIYRIYGKQYTPQQISAFLL
                     QKIKRDAEAFLGEPITKAVITVPAYFNDNQRTATKDAGKIAGLDVVRLVNEPTAASMA
                     YGLDRSGEYKILVFDLGGGTLDVTIMEFGGGTFTVLATSGDTQLGGTDMDNAIYEWIA
                     GEFKKLEGIDIRTDKMAINRVREAAEKAKIELSTVIETEINLPYVSATQSGPKHLSMK
                     LSRAKLEQLVEPIIKRCLQPFEQALKDAKLTKNDIQKVILVGGPTRMPIVQKFIEDHI
                     GKKVERGIDPMECVAIGAAIQGAILGGEITDMVLLDVTPLTLGLETLGGVRTELIERN
                     TTIPTKKSQTFSTAADYQTSVTIHVLQGERPMAADNVSLGQFNLVGIPPAPRGVPQVE
                     VTFDIDASGILNVSAKDMGTGKEQKMTITASTKLAENDVRKMVNEAKQFEEQDKVRKE
                     EVEARNSADSLVYIAEKTKSDLAGKLSTELVEKVNAAIVSVKSALEGKDSAKIKAETE
                     KLQKVLGEAGSAAYQQASQKQAQQETGHEAGPAGAANQETGGKSDENVVDADFKVHDE
                     K"
     gene            complement(7406..7897)
                     /locus_tag="VFC43_08210"
     CDS             complement(7406..7897)
                     /locus_tag="VFC43_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HZK31195.1"
                     /translation="MTEDLEQVQKDLENQTRLAEDRFNRLKYLQADFDNYRKWSEKEK
                     ASIIALANENLIKDLLVILDDFEQAMPSLGQEKNRQGIQMIQRKMQKILKEYGLESIE
                     GVGKKFDPYFHEVICREKCNEEPNTILEDFQKGYQLRSKVIRPSKVKIAEHDTEHEGE
                     NNG"
     gene            complement(7894..>8075)
                     /locus_tag="VFC43_08215"
     CDS             complement(7894..>8075)
                     /locus_tag="VFC43_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31196.1"
                     /translation="LFQRYGASCLTIGYGRCELPDCYYVNLSGIPGIDPIAIDKKPDL
                     GEEPIRDDQDKMRKQ"
BASE COUNT         1736 a         2151 c         1820 g         2368 t
ORIGIN      
        1 ttcggtgacc attgtccgga tatggtcaag ctccgttcca tcaccctggg gatacctgcg
       61 caggaggaga ttatacaggg aaggggcgag tgccatgcaa tatccgttgt gaaatcctgc
      121 ttcatccagt ttggtgactg cttctatgat ctggtccgct gcagtgccta tcttatccca
      181 ttttgtcagg gtctgggacc ctgcaccttc aacgttcatc agaccggacg cacccccgct
      241 gccgttgaac agtatctggt cctcttttgc ggcgcaatcc attgctgcat ttgccacagg
      301 tcctccatcg aaagcaagtt tatctttttc aaaagcagca aggtcacgct ttcccaggct
      361 gaatccggat tttatgatgc ttaaaggaac aaaactgctg gtatataccc cttcttccgt
      421 ttcgtagtct ccgagtggca ggatcttttg tccaaaacca aacggccctt caagtgtaag
      481 caatcgccga ccagcaagct gactttttgc tgcttcgatc atcattgtgt cgagcaggtg
      541 ccacgtatca gcaccgatcg gtgcttcatt gcgtccaaga aacatatttt ccattttaat
      601 cacctttttg tgctggtttt aatgatccca ccttcggggc atcctgttcc tgctttacct
      661 cagcccgcgg tgcaactttt gcataaaacg catccacctc aagggcaccc ttttgtaaaa
      721 atgcatcttc atcgccggtc aggattgcaa gaagacgggc aaattcaccg gcatgaaccc
      781 tctcttcatt ggctatgtca atcaatactg cttttacaac agggttatcc gtcgcatctg
      841 catgtgccat atatgtgtgc acagcttcgt gttcagctga aaggtccagg cggattgccc
      901 ggataagttc ctgcgttgtt aatgttcggt ccggtatttt tccgctgaaa ggattgacaa
      961 attctggcat tgttcaattc tcctctcata ttattttgtc atcaggtcaa accgtttgct
     1021 caccatatcc cagttcacga tattccagaa tgactcgaca aattttgcgc gatcattctt
     1081 gtaatccagg taatacgcgt gctcccacac gtcgagtacc atcaggatgt gaaaaccggg
     1141 aataatattc acattgtgtt tctcgatctg catgatcaga ggccttttgg ttttctcaca
     1201 gtaagtgata actgcccatc cggatccctc cacgctggtt gcggtctggg taaactcctt
     1261 tttgaaccgg tcgaatttcc cgaattcgcc gtcgattgct tttgccagat cacctttcgg
     1321 gatcccgcca ccaccctttc cggcaggtgc aaggttttcc cagaaccggt tgtggagccg
     1381 gtaacctccc atgtggaatg acaatgcctt gagtactgcc ttaaaatcaa ggtctgcatt
     1441 atctttccgg gctttgtcca gtttttcaag gattgcgttt gcacccgtga catatgcctg
     1501 gtggtgtttc aggtggtgca gtttcagctg ctcttcagaa atatacggcg caagcgcagt
     1561 atactcatac ggtaactttg gcaatgaata cagttttact ggttccatac atcaggacct
     1621 ccgttttttt tcctgctatc acaggtgtgg aattttcacc tgatgatggg gatattgaaa
     1681 atgacctttt atagaatatt gcccacaact cagattttga aacaaatcct gagacaaaat
     1741 caggtctctt taaaaacaca cacagtccac tgtgaaaaac cgcaataatg atgggagtga
     1801 cgcttcgcta caagtgaatc gcctgggcga tgttcatggt tcccggtctg gagcccgtcc
     1861 tcccataagg gaggccatca aaaaaatgta tacctatcac ctggaccggg ctccctggtt
     1921 ggtgacaaaa acattcagtc cgttgtgtat tattaaaaaa agttattggt gtggttcaac
     1981 aagaacagtt atgtctgtct ctttttcatg actgattctg caattttccg gacaaattca
     2041 tttttatccc gtgtggctgc ttcaagatac agaaaactct tctggtcccc gattgcggca
     2101 agtgcaagcg ctgaacccat ccttacccgc cagtcaggat ctttcagtac atcaccgagg
     2161 cggtctgcag aagcaggatc cgctatgtgg acgagagcgg caacagcatg tcttcttacg
     2221 tcctggttct catcccgtaa caagagtaaa atggcgtccg ttgccttttg tgcccgaatt
     2281 ttaccaaggg agtcggcggc tctcatccga acagcccagt cgccatcacg aagggtgtag
     2341 ataagtcgat ctactgccct ctggtcacct atctggccga gcgcccaggc agctctcagc
     2401 cgaaccacgc tgtccttatc gtcaagggct ggtataagaa gatctacggc aggaacccct
     2461 atccttgcaa gggcaaacgc cgctccccac cgcatatctt cgtttttatg agataagtcc
     2521 cggattaatt cctgtattct ggtttgagaa atgtccaatc cttctaccat gagttaacct
     2581 ccgttaagat attgatacgg tctttctagt ccgatcgttt ttttaccgca gtatcatata
     2641 ccatccagtt cctctcccac tcggggtttt gtgatttgag cattgccaga acttttcttt
     2701 tgatctccag cgtggtagtc ccctctttta catttctctc tatctcgcca gcaattgtcc
     2761 gagcatagtc cgggtctgca ccggatttaa cagcactgac aatgatcttc tcgcgtacga
     2821 atggttcttt tctgccgtct tgttttttta tcataatcat agcaactcac cagatttttc
     2881 ttttcctgaa aactacccgc cttgtccccc ttaatggcgg tttaaaacgt tttttgtgcg
     2941 aatcctgcat gaatttaatc aatgggaata ttccctttgg cggtttttgc agtcttcttg
     3001 agatggactt caagcacacc attttttacc gttgcagttg ctttatcatc cgtcactggt
     3061 aagggaagcg gaatgatcct tgtcatggaa ccaaaccttc gttcacgcag gtaatatccc
     3121 tcctcttctt cttttttctc ttcttttcgt tcccaggaaa tttccagcgc ctggggattg
     3181 atcaggttaa gcgtgatatc ttttttggta accccgggaa tcagatctgc cgtgatgaca
     3241 acttcatcac catgtacaga aacgtcaacc ccaagccggg gatgatgcgc cggaagcatt
     3301 ttcattgcgg attcccggcc ggaggggagt agggcggttg ggttggtttc atacatctga
     3361 tgatagagtg catccatgta atttctcatt tcatccagct catcaaagat tgacttgtaa
     3421 taccatcgca ccataactca ttcatcacat cctttttttt catggcggcc cccgccgggt
     3481 tcatgtacta ctaactgcct gatgacatac ctcgtgcctg cataccctcg tcaaacgtca
     3541 ctgtctgttg aaggatcttt tatcagctaa aaaatgccat cagagttgtt catgcgtggc
     3601 ccgcaatcct gtgacaaaaa ggatattata cagaaaattt tatcaaatgt tttacgtaaa
     3661 tcagattttg aggcaaattt tgcaacaaca tgatcgctga taatcgtgat gaggagcaat
     3721 atcacctgtt tttggaaagc agctcatcag ttgtcgccct cacccattgg tgatggtctt
     3781 cccagatttc ccatatggct tccatccatt tcctgatcat tgcatctttc tgcctttctg
     3841 tctcttcacg caacacgtct gttaccgtta aaaccgcgga ccggacaggc ggctcgagaa
     3901 acggccagtc cttttttatt tttgcaattt tcatgtgagc cttctgaact tccctgccgg
     3961 tatatccttt ctccagcgca aggtgcagcc cgatcaggcc gaatgcgacg gcgatgggcc
     4021 gggtcagtcc gccggcatgc tgcgcagtgt acgcatcgac cacatgctga tggataaatc
     4081 cgggatcctg ttttgaaaca gtgtaacact gcaggtagga aaaagcctgc cagcattcac
     4141 ccgaggcatt caacctgccg tccggatcga gatgcatatc cggcaacctg acccggcacc
     4201 ccgggcattc agtcatcctg actgcctgcc tgctccatgt cacttcaaga accggatttg
     4261 acggttgtga gaaaaaaagg tatggctttt tttatacagg aaggaaaaaa tttgcgaaaa
     4321 gaataaaatt gtatgttgaa tttatcaaaa cagctgaata cccgtgcagg acttttccga
     4381 ccgtttaatg tattgaaccg gcaggttctc ctgcatccct tttacctgca ggttcaggct
     4441 gattttctgc gcatatgatg cttcaaccta caaaggcttc tttcatcagt tcttcctgct
     4501 tttttgttag attttcagga attttcacaa tcactttcat gagaagatcg ccccttccgc
     4561 tggaattcag gtacggcatt ccctgctctt tgaggcggaa aagggtatga ctctgcgtac
     4621 cccggggtat tttcagtgtt gcacttccgt tgatggtggg gactttgatc tcacctccaa
     4681 gcaacgcagt cgcaagcccg acaaccgctg tgctgtacaa atccgctccc tgccgttcaa
     4741 aaacaggatg cttctttata tggacaacgg catacaggtc gcccggcggg ccgtggttct
     4801 cccccggctc tccctccccg gcaatgcgta agaactggcc atcttcaaca ccggcaggaa
     4861 ttgagacctc aatccttctc gtcttcttta tcgtccctct gccctggcag gtctcacacg
     4921 gcttgccgat gattgtcccg cggccaccac atccggggca ctgggcgata ttcattacct
     4981 gctggcggcc actccgctgg atggtccgga tctcgccgga acccccgcac tggttacagg
     5041 tacggctgaa acccggttgt gcaccggaac catggcaggt actgcactcg tagctgtggg
     5101 ggacttcaac agagtttttg gtaccatagt acgcatcgga aagggagatc tcgatctcat
     5161 acctcaggtc agcgccggcc cggctccgca cccgcccgga acctgccgag tacgcatcga
     5221 agatatcacc cagcccaaag tccctgaaaa ggtcgtcgta actgggagtt ttatacccgg
     5281 cagtgtcccc aggtttaaaa gcagtatgtc ctacctggtc gtactcagct tttttctgcg
     5341 gatcgctgag cacctggtag gcctcgttga tctccttgaa cttctcttct gcttctttac
     5401 tgcccttgtt gaggtccggg tgatattttc tcgccagctg cctgaacgcc ttttttaagt
     5461 catcctgcga tgcatcccgt ttgacaccca gggtctcgta ataatctttt tttcccattc
     5521 gtgatcgaag accggttatt tctcgtcatg gaccttgaag tcggcgtcaa cgacattttc
     5581 atcgcttttg ccgcctgttt cctggtttgc agcgccggca ggacccgcct cgtgaccagt
     5641 ctcctgctgg gcctgtttct gggaggcctg ctggtatgcg gctgacccgg cctcgcccag
     5701 aactttctgg agtttctcgg tctcggcctt gatcttcgca ctgtcctttc cttcaagcgc
     5761 tgatttgact gaaacaatcg ctgcattcac tttttctacc agttctgtgc tcagcttccc
     5821 ggcaaggtcg gattttgttt tctctgctat atacacaagg gaatcagctg agttccgggc
     5881 ctcgacttct tcttttcgta ctttgtcctg ctcttcaaac tgttttgctt cattgaccat
     5941 cttcctgaca tcattttctg ccagttttgt ggaggcggtg atggtcattt tctgctcctt
     6001 tccggtgccc atgtcctttg ccgacacatt aaggatgccc gatgcatcaa tatcgaacgt
     6061 gacttcaacc tgggggacac cgcgcggtgc cggaggaatg cccacgaggt tgaactgccc
     6121 gagactcacg ttatccgcag ccatgggccg ttctccctgg agtacatgga tggtgacgga
     6181 agtctggtaa tctgcggcag ttgagaatgt ctggctcttc ttcgtgggta ttgttgtatt
     6241 tctctcaatc agctcggtcc tcacaccacc gagggtttca agaccaaggg tcaggggtgt
     6301 gacgtcaagg aggaccatat ccgtgatctc tccacccaat attgcgccct ggatcgccgc
     6361 tcctatggca acgcattcca tcgggtcgat gccccgctca accttctttc cgatatggtc
     6421 ttcgataaat ttctggacaa tgggcatccg ggtcggacca ccgacaagaa ttaccttctg
     6481 gatatcattt tttgtcagct tcgcatcttt caatgcctgt tcgaacggct ggagacagcg
     6541 ttttataatg ggttcgacca gttgttcgag ttttgcccgg ctgagcttca tggagaggtg
     6601 ctttggcccc gactgggtgg cagatacata cgggaggttg atctcggtct caataaccgt
     6661 ggataattca atctttgctt tttctgcagc ttccctgacc cggttgatgg ccatcttatc
     6721 agtcctgata tcaatgccct cgagtttttt gaactctccg gcgatccatt cataaattgc
     6781 attgtccata tcggtgccac ccagctgggt gtcgccggat gtggcaagca cagtgaatgt
     6841 cccgccgcca aattccatga tggttacatc aagagtcccg ccgccaagat caaagaccag
     6901 tatcttgtat tcaccgctcc ggtccagccc gtatgccatg gatgccgccg taggttcatt
     6961 cacaagccgt accacatcga gaccggcaat tttaccggca tcttttgtgg cggtccgctg
     7021 gttatcattg aaataggccg ggaccgtgat cactgctttt gtgatcggtt ctccgaggaa
     7081 cgcttcggca tcccgcttga tcttctgtaa gaggaatgcg gaaatctgct ggggagtata
     7141 ctgcttgccg taaatccggt aaatctggtc ggttcccatc tttctttttg cagccgtgac
     7201 tgtaccttcc gggttgctta tggcctgcct gcgggccggt tcccctacaa ggagctgtcc
     7261 gtcaggagta aacgccacat aggaaggaaa cattttcccg gcaaccgttg ccccttctgc
     7321 cgaagggatg atggtaggtt tgccgccaag catgacagct gcctcactgt tggaggttcc
     7381 gagatctatt ccgataattt tttctttacc cattgttctc accttcatgt tctgtatcat
     7441 gttctgcgat cttcactttc gatggcctga tcacctttga tctcagctgg tagcctttct
     7501 gaaaatcctc aaggatcgtg tttggttctt cattgcattt ctccctgcag ataacctcgt
     7561 ggaaatacgg atcgaatttt ttcccgacac cctcaatgga ctctaaaccg tattctttga
     7621 ggattttttg catctttctc tggatcatct ggatcccctg ccggttcttt tcctgtccaa
     7681 gggatggcat tgcctgttca aaatcgtcca ggatgaccag cagatctttg atcaggttct
     7741 cgtttgccag tgcgatgatc gatgcttttt ctttttcaga ccacttgcgg taattatcaa
     7801 aatccgcctg aaggtatttc agccggttga accggtcttc tgcaagccgg gtctggtttt
     7861 caagatcctt ttgtacctgt tccagatctt ctgtcattgt ttcctcattt tatcttgatc
     7921 atccctgatc ggttcttccc ctaaatccgg ttttttatca atcgctatgg gatcaattcc
     7981 aggaattccg gataaattca cgtaataaca atcagggagc tcgcagcgac catacccgat
     8041 tgtcagacat gatgctccgt accgctgaaa tagag
//
LOCUS       DASRLV010000213        10068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_239954,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000213 DASRLV010000000
VERSION     DASRLV010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 10068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10068
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239954"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(367..1236)
                     /locus_tag="VFC43_08220"
     CDS             complement(367..1236)
                     /locus_tag="VFC43_08220"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HZK31197.1"
                     /translation="MLGLLVLAIAPAVFILWYVYRKDYYEPEPLRLVIQVFLLGGLSV
                     IPAAVIEYPFPSGIITSSVVAPFVEEIIKFCVVFFFVYRLPEFDEPVDGMMYAAAAGL
                     GFAAVENVFYVLEGGIAVGVLRAIASVPGHMIFSCIWGYALGTAKFRPGSAWRPIVIT
                     GLAGSIVLHGIFNFSLEVYEVAGLVFILFFLIPLGLWYTRKNISKAHADPASAFSLLQ
                     RSSVNGTGILPWNSGSGTDSYPSGPQSASPSTASSNTGRDQDGTRTVHYCTSCGTRVI
                     DEERFCRNCGKEL"
     gene            complement(1285..1845)
                     /locus_tag="VFC43_08225"
     CDS             complement(1285..1845)
                     /locus_tag="VFC43_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HZK31198.1"
                     /translation="MEKTDSSENPPKGGKSFHGQHLIENIFERALWNVRYIVLLGVIF
                     GALSAIVLFIAGSSEIYSILAGYFQTGPHVLTHEEILVGIIGAVDFYLIGLVLIIFSF
                     GMYELFISDIDIARIEGGFGSLLDIKNLDDLKNKIIKVIIMVLIVTFFQRILSMVFTT
                     SLDMLAMAVSICLICVGVYFLGRQGH"
     gene            complement(1945..3063)
                     /locus_tag="VFC43_08230"
     CDS             complement(1945..3063)
                     /locus_tag="VFC43_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HZK31199.1"
                     /translation="MTIGTEHEYSINDNRFNPLPVSDQIIKSLCGRVESEILFGNVKL
                     SKELQKTVLEIIPRTPSHTLIALEGQLVSGVRKFYHVFRDRYVLLGLGMHPLLTLDRT
                     AVWDHDEREYYEVYDRLFNIYQHGWLNIQSLQINLSYSSEKQVVMIHNRLRPLLPYLI
                     AVTASSPMVEGGLTGIADNRLRYYRDNQKEIPLICNRIIPDKIRSLDDYRSIQQDVFG
                     ELRNRGEDILCEEWVNSSGLIIRFSRNCVEIKALDEQECIRSDMAVCAFVQSLMRCTA
                     LPVETDQDDILARTEEAITCGTSAFRPELERLYDAAWNHANEDERVYLPVILDRIRHG
                     SLAELIRDRFSKEGEMIPILSEMAACLKSNSPYRGQKM"
     gene            complement(3047..3520)
                     /locus_tag="VFC43_08235"
     CDS             complement(3047..3520)
                     /locus_tag="VFC43_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HZK31200.1"
                     /translation="MRCGGHCCVDAHPPVSRSCSERLLAAGISTDMFEYTGYKRLKVK
                     ENGVCILSEQGKCRIHTVKPETCRAGPFTFDVQGDVIVIFIKYERICPLVKLLKEIPE
                     AYQQQYNLAVKSITNLVSNLSCDEIDAICCIDEPDTEKVSELPRKKSDMIYDNRY"
     gene            complement(3566..4438)
                     /locus_tag="VFC43_08240"
     CDS             complement(3566..4438)
                     /locus_tag="VFC43_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HZK31201.1"
                     /translation="MSRLGIFVDRKTLSNSEQLNALIRCRDVAEGMGHDVDFILPVDI
                     HKIPAVDALFIRARTDPMNVTYVAARMASFYNIPVIDDPRSIQICSDKVNMYSHLDKK
                     HISMPKTIFLSKHDLTVERVMDIFDDLGSPLIVKEPSTSFSLRVEKVQDSAEFFRVAR
                     RFIKLSDWIVVQQYVESTYDWRVGVLGGRLLYVCKYTIPSDTFKIQASVNGHLVYCGV
                     ESVTPDKVPPHVIRLGIDAANAIGTGLYGVDIKNNNEDAYVIEVNDNPSIESGEDDCY
                     PHVFDQIVTHLFTM"
     gene            complement(4435..5226)
                     /locus_tag="VFC43_08245"
     CDS             complement(4435..5226)
                     /locus_tag="VFC43_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31202.1"
                     /translation="MNRIRSDKIPKKYSDPALPDGLVPATGSIIPEDNLVSNDPSYQI
                     TLPKTNGKRDPNIVKDSLFTLKRNEVFHIISENYAYKTETYYSILHHELKGEPVQPTS
                     RAILDAYVVPLSLERAKLAGIPVCTWGISQGYIPLPAILYGLNYFATPSDYFVVRDMD
                     QAKEVIKHVTNKGKYPFCYQKLNDNATIHSCVGIFGKTTSPGPDIPTLVQKVYEQFLL
                     PLVTLVFVKTEDSYLLSSLSPTKYSHLSAEERTILNAYLSGQEFL"
     gene            complement(5223..5447)
                     /locus_tag="VFC43_08250"
     CDS             complement(5223..5447)
                     /locus_tag="VFC43_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31203.1"
                     /translation="MNECLTIDYEDEEDHRWFFSCRNPDQNSDTRTPADGDEIFFVNF
                     YSDIIFLVHSVEKQVLFAISQKPDFRAVSG"
     gene            5682..6236
                     /locus_tag="VFC43_08255"
     CDS             5682..6236
                     /locus_tag="VFC43_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZK31204.1"
                     /translation="MHIPTPGELRGRREALGMKQTELARRAGISQSMIARIEAGNVDP
                     RVSTLSKIITVLNSAEPKKIRAAQIMHTPVLSVQPQDAISRAVEIFEKNNISQLPVIE
                     RGIPVGCISETVIVKAIEQQRLHKTHLFFVKDFMEPGFPTVPPDMDVETVINILQQNH
                     AVLVIEGRLVIGVITKHDLISLIV"
     gene            complement(6552..6728)
                     /locus_tag="VFC43_08260"
     CDS             complement(6552..6728)
                     /locus_tag="VFC43_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31205.1"
                     /translation="MKIIKIHIGEGSPPQGLSEARRGKEVFAFTVPIIAAVASLFGAA
                     AFIIIMRKPLSNNI"
     gene            complement(6798..7466)
                     /gene="tpiA"
                     /locus_tag="VFC43_08265"
     CDS             complement(6798..7466)
                     /gene="tpiA"
                     /locus_tag="VFC43_08265"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HZK31206.1"
                     /translation="MPSPLILINTKTFKEGMGNRAHMIANAAQLVAHESGVTIGLAPS
                     FFDLHPISHHFAIPVYAQHVDGFEPGAHTGHITADALKIAGAAGSLINHSERRLTLAD
                     IESSVRALQAQKLISVICSNNEATSACSAALGPEYVAIEPPELIGSGVSVSKANPDII
                     RRSVAAVHAVNPKVKVLTGAGIQSGDCVKIAVDLGTDGVLLASSVVKAKEPVLVLRDL
                     VSKL"
     gene            complement(7569..9248)
                     /locus_tag="VFC43_08270"
     CDS             complement(7569..9248)
                     /locus_tag="VFC43_08270"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HZK31207.1"
                     /translation="MGRDAPDNRGFCIGVVNRYDEKSKSVKIRTTQSIIPQRGDGLLF
                     RHKKNPSHEFGFSLNSIPVEKDGEISLRVPQPVEPGTGVFITSSPAFEQRARRIITHP
                     TPGLRHPVLVDADVTIDALGRLEIKGLVHSRIAGKFQVTYSPDFNLEPALTHPLTPEQ
                     IELHVKKSKDTPFTISNFTVHSEGNWFLTIARLNYVRREFLKIAEDMIVARARPAQEN
                     IDQSSCRWNEHKMHGVSKSLAARNSSTPARLALGVYADSIEGVQGALEGGCDFICFEP
                     VFVPGTSTCRKISVHSSFEAQIVTVSAMCNDAGVRFVLKFPRITRNNYLKGVLPFLSK
                     GTDLNISGVLVENYGTAYALMQATPHIGLFGSGGLNIYNHQSVYQSAPIFRSVTLSPE
                     LSRDEMELLIRRVRSQGCLTSFSLIVQGNSEAMVSEDCILQPWEKEVQKNKDRHHPRF
                     YGIRDSTGHIFPVRVDGECRSHIFNSSELCLIDHLPSLLQMGINEVIIDTRGRTTGYA
                     RDMTGIYTKAIALVHDGIKTPDKRLELLKDTVKLYALGGITAGHYIRGLKE"
     gene            complement(9321..9959)
                     /locus_tag="VFC43_08275"
                     /pseudo
     CDS             complement(9321..9959)
                     /locus_tag="VFC43_08275"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
BASE COUNT         2711 a         2378 c         2343 g         2636 t
ORIGIN      
        1 ccttcaaagc atttgtcttt gttatttcat ataatgataa aaggaatttc cataataatc
       61 attgtttatt ggatgtgatg ttgaaattgg taatttcata tcaatcaatc atgataaata
      121 ccgccgcccc cggaggcaca cccccgcggc ggattaatga ctaaaaacac cctggccgct
      181 gtgcctaagg agaggccctg aggcgggggt caatcgtata gataaccgat tttgatgatt
      241 tgggtgtaaa cgccctcttt tcctgtcagt ttcttttaga tctgattatc cgtttccatc
      301 atgtgaagca gtcctgattg ggttcaatca aagaatccgt gtatcttttt accaggtaat
      361 tttacctcag agctcttttc cacaattcct gcaaaacctt tcttcatcaa taacacgtgt
      421 tccacaactg gtgcaataat gtaccgtcct cgtgccatcc tgatcgcgac cggtatttga
      481 tgaagcagta gagggtgagg cagactgcgg acctgacgga taagaatctg ttccactccc
      541 tgaattccat ggtagtattc cggttccgtt gacagaggat ctctgcagca gcgagaatgc
      601 tgaagcggga tcagcatgcg cttttgagat gttcttccgt gtataccaca atcccagcgg
      661 aatcaggaaa aacaggataa aaaccaggcc ggctacctca taaacttcaa ggctgaagtt
      721 aaagatcccg tgcagcacga ttgagccggc aagacctgtg ataacaatgg gtctccatgc
      781 cgacccgggg cggaattttg ctgttcccag tgcatatccc cagatacagg aaaagatcat
      841 atgtccgggg actgaggcaa tcgctctcag aaccccgacc gcaatccctc cctcgagtac
      901 atagaagaca ttttctactg cagcaaaccc aagaccggct gcagcagcat acatcatccc
      961 atcgaccggt tcatcaaatt cggggagacg gtaaacaaaa aagaaaacaa cgcagaattt
     1021 tataatttcc tcgacgaaag gggcaacaac agaagacgta attatgcctg agggaaaagg
     1081 atattcgatg accgcggcag gaattacact gagtcccccc agcaggaata cctggattac
     1141 gagacgcaaa ggctcaggct cataatagtc cttccggtat acgtaccaga gaataaagac
     1201 cgcaggggca atagcaagca ccaggaggcc taacatcagt caacaatgag taagcataag
     1261 gaaaaagggt tcggttttta aattttagtg cccttgtctt ccaagaaaat atacaccaac
     1321 acaaatcagg catatcgaca ccgccattgc cagcatatcg agagaagtgg taaacaccat
     1381 ggacagtatc cgctggaaga acgtcacaat caataccatg atgatgactt ttatgatctt
     1441 gttcttgaga tcgtcaaggt tctttatatc caggaggctc ccgaacccac cctcaatccg
     1501 ggcaatatcg atatcagaaa taaagagttc atacatacca aaactgaaga taatgaggac
     1561 aagcccgatc agataaaagt caaccgcccc gataattccg accaggatct cctcatgagt
     1621 aaggacatgt ggccctgtct gaaaataccc cgccagaatt gagtatattt cacttgatcc
     1681 ggcgatgaac aggacgatcg cagagagtgc tccaaagatg acacccagaa ggacgatgta
     1741 gcggacgttc cagagagccc gttcaaagat attttcgatg agatgctggc catgaaaaga
     1801 ttttccaccc tttggcgggt tttctgaaga atctgttttt tccataagat atcctttacc
     1861 gcatcagata ttatcccggg gaggataact tttttttcac tataccggaa attcgccgga
     1921 gtatggcgga atgtcgacct gttctcacat tttctgacca cggtaaggag agttcgattt
     1981 caaacacgca gccatttcag atagaatggg gatcatctct ccctcttttg aaaaccggtc
     2041 acggataagc tctgcaaggc tcccgtgtcg aatcctgtca agaattacgg gaaggtacac
     2101 ccgctcatcc tcattggcat gattccatgc tgcatcatag agccgttcta gttccggcct
     2161 gaatgctgaa gtcccacagg ttattgcctc ttcagtacgt gccagtatgt catcctggtc
     2221 cgtttcaacc ggaagagcag tacacctcat cagcgactgg acaaatgcac agacagccat
     2281 gtccgatcgg atgcattcct gctcatcaag agccttgatc tcgacgcagt tccgtgaaaa
     2341 acgtatgatt agaccactcg aatttaccca ctcttcacag agaatatcct cgccacggtt
     2401 tcgaagctcg ccaaaaacgt cctgctggat ggaacggtaa tcgtcaagac tccggatttt
     2461 atcgggaata atccggttac agatcagggg aatttccttt tggttatccc ggtagtacct
     2521 gagccggttg tcagcaattc cggtaagacc cccttcaacc atcggcgatg atgcagttac
     2581 cgctataaga tacggcaaaa gaggcctgag acggttatgg atcataacca cctgcttttc
     2641 gcttgagtag gagaggttaa tctgcaggga ctggatattc agccacccat gctggtaaat
     2701 attgaagaga cggtcgtaaa cctcgtaata ttcacgttcg tcatgatccc agacagcagt
     2761 gcgatccaga gtgagcaggg ggtgcatccc gagtccgagg agtacatacc ggtcacggaa
     2821 aacatggtaa aacttccgga ctccagaaac gagttgtcct tcaagggcaa tgagtgtgtg
     2881 cgagggagta cggggtatga tctcaagcac ggttttctgc agttctttac tcagtttaac
     2941 attgccaaac aggatctctg attcaaccct cccgcacagg gatttaatga tctgatcgga
     3001 aaccggcagc gggttgaacc ggttatcatt gatagagtat tcgtgctcag taccgattgt
     3061 catatatcat gtcacttttt tttcttggga gttcagaaac cttctcggta tccggctcat
     3121 caatgcagca gattgcgtca atctcatcac aggaaagatt gctcacaagg ttcgtgatac
     3181 tttttactgc cagattatac tgctgctgat atgcttccgg gatctctttt aagagtttta
     3241 ccaaaggaca gattcgttca tattttatga aaatcacgat gacatctccc tgaacatcaa
     3301 acgtgaaagg cccggcccgg caggtttcgg gtttcacggt atggatcctg cacttgccct
     3361 gctctgaaag aatacacaca ccgttttctt ttactttgag acgtttatat cctgtatatt
     3421 caaacatgtc cgtagagatt ccggcagcca gtaacctctc ggaacaggac cgcgaaacgg
     3481 gagggtgtgc atcaacacag cagtgaccac cgcaccgcat acaaatgtct tcagcccgta
     3541 ataaccagtc ttcagtcaaa aaaaatcaca tcgtaaaaag atgcgttaca atctgatcga
     3601 atacatgggg ataacaatca tcttcaccgc tctcaatcga cgggttgtca ttgacttcaa
     3661 taacatatgc atcctcgtta ttatttttta tatcaacgcc gtaaagtccc gtgccgattg
     3721 cattcgcagc atcaatcccc agccgtatga catggggtgg caccttatcg ggagtgacac
     3781 tttcaacccc gcaatagaca agatgcccgt ttaccgatgc ctgtatcttg aaggtatcag
     3841 aggggatcgt gtatttacac acatacagca gcctgcctcc cagcaccccg accctccagt
     3901 cgtatgtgct ctcgacatac tgctgcacaa cgatccagtc tgataacttg ataaaacgac
     3961 gggcaacccg gaaaaattca gcactgtcct gaactttttc cacacggagg gagaacgaag
     4021 ttgagggttc ttttacaatg agcggagatc caagatcatc gaaaatgtcc ataacacgtt
     4081 cgacggtgag atcgtgtttt gacaaaaaaa tcgtttttgg cattgaaatg tgttttttgt
     4141 caagatgtga gtacatattc actttatccg agcatatctg gatggaccgg ggatcatcga
     4201 taacgggaat attataaaaa cttgccatcc gtgccgcaac ataggtcaca ttcatcggat
     4261 ctgtccgggc acggatgaaa agtgcatcga ccgccggtat cttatggata tcaaccggta
     4321 atatgaagtc aacatcgtgt cccatacctt ccgcaacatc cctgcaccgt atgagggcat
     4381 tgagctgctc tgaattcgag agcgttttcc ggtcaacaaa gattccgagc cgactcataa
     4441 gaattcctga cctgaaaggt atgcgttgag aatggttcgc tcttctgccg aaagatgaga
     4501 gtatttagtc ggggacagag aggaaagaag atacgagtcc tctgttttta caaaaaccag
     4561 ggtgacgagg ggaagtaaaa actgttcgta cactttctgg acaagggtgg gaatgtcagg
     4621 accgggtgag gtggttttgc caaaaatacc cacacatgag tgaatcgtag cattatcatt
     4681 tagtttctga tagcagaatg ggtacttccc cttgttcgtt acgtgtttga taacctcttt
     4741 tgcctgatcc atatccctga cgacaaaata atctgagggt gtcgcgaaat aattgagccc
     4801 ataaaggatt gcaggaagcg ggatataacc ctgcgatatg ccccatgtgc acacgggtat
     4861 gccggcaagt tttgcccgtt ccaggctgag gggcaccaca tatgcatcga gaattgctct
     4921 gcttgtgggt tgaacaggtt ctcctttgag ttcatggtgc aggatggaat aataggtctc
     4981 agttttatac gcgtaattct cacttatgat atggaatacc tcatttcgct tcagagtaaa
     5041 aagggaatct tttacgatat ttggatcgcg ttttccattc gtttttggca aagttatctg
     5101 gtaagatggg tcatttgaca cgaggttatc ttccggtatg attgatccgg ttgcagggac
     5161 caggccatcg ggtaatgccg gatcagaata tttttttgga attttatcgc tcctgattct
     5221 gttcatccgc tgaccgcccg gaaatccggt ttctgagaaa tggcaaaaag aacctgcttc
     5281 tctacagaat gtacgagaaa tatgatatca gaataaaagt ttacaaaaaa aatttcatcc
     5341 ccgtctgccg gagttcgcgt gtctgaattc tggtcaggat tccgacagga aaagaaccac
     5401 ctatggtcct cttcatcctc gtaatcgata gtgagacact cgttcatggt caacactaat
     5461 ccaattttta attcattgga ttatcgctga tagctatatg ccttgttctt taaagaattt
     5521 tgcagggatt ctgctgatat tactattatt cataacaatc agaaaaataa tgaccggtaa
     5581 ttaaattccg ggatttgtta ttctatggat ataatccagc agctgctgaa gtcataaatg
     5641 atcataaaat taataatatt ctatcaccat cagtaatcac catgcatatc cctactccgg
     5701 gagaactccg cggacggcga gaagcgctcg gcatgaaaca aactgaactt gcacggcgtg
     5761 cgggtatcag ccagtccatg atcgcccgca ttgaagcagg aaatgttgac ccccgggtaa
     5821 gcacactatc gaagatcatt accgttctga atagtgctga accaaaaaag atacgtgcgg
     5881 cccagataat gcacacaccg gttctttctg tccagccgca ggatgcaatt tcccgtgcag
     5941 ttgaaatttt cgaaaaaaat aatatctccc aattaccggt aatcgaacgg gggatccccg
     6001 tcggatgcat ttctgaaaca gtcatcgtga aagcgatcga acaacagcga ctgcataaaa
     6061 cacacctttt ctttgtcaag gactttatgg agccgggatt tcccacggtc cccccagata
     6121 tggatgtgga aactgtgata aatatcctgc agcagaatca cgcggtgctt gttattgaag
     6181 gaagactggt tatcggggtt atcaccaaac atgacctgat ctctttgatc gtatgaatgg
     6241 ttggattttt tttaaatacc gataagaaaa tccaccttct ttcaactgca aaaataccgg
     6301 atttagtatt cgtaccttcc ctgatggcga taaaaaggct ctgtagaatt cctttgacca
     6361 ttgtatgaaa taaaaaggac agatgcttta aaggagggga cagtgcctgt cccccggacc
     6421 gcccctacgc tgtgcgatac tatggtatta ccttctcagg agttatcgca ttggggggag
     6481 cccaggttga gggggtgaaa cccccatgaa cgttcaggtt ttttgtacat ttcccatgaa
     6541 aatcagatta ttcagatatt attagaaagt ggttttcgca taattatgat gaacgccgcc
     6601 gcaccaaaaa gggacgccac cgcggcgatt atagggactg taaatgcaaa aacctccttg
     6661 ccccgccgtg cctcggagag gccctgaggc ggggagccct ccccaatatg aattttaatg
     6721 attttcatgg tttaggtaag aactcccgtt cgtgcccgtt tctacagaac caataaaaaa
     6781 aagtatattt aaatggttca gagttttgaa acaagatcgc gaagtactaa taccggttct
     6841 tttgccttga ctacgcttga tgcgagcagc actccatcgg taccgaggtc cactgcgatt
     6901 tttacacaat caccggactg gatacctgca cccgtcagta ccttcacctt tggatttact
     6961 gcatgaacgg ccgcaactga tcgcctgatg atatccgggt tggcttttga taccgatacg
     7021 ccactcccta tcagttccgg cggttcaatt gcgacatatt ctggcccaag tgctgcagaa
     7081 cacgcactcg ttgcctcatt attcgagcag ataaccgaga tgagtttttg tgcctgaagg
     7141 gcccggactg atgattcgat atcggcaagg gtaagccgtc tttctgaatg gttgatgagc
     7201 gatccggccg ctccggcgat tttcagagca tcggcagtaa tatggcctgt gtgagctccg
     7261 ggttcaaacc catcaacatg ctgcgcgtat accggaattg cgaagtggtg actgatgggg
     7321 tggagatcaa aaaaagaggg ggcaaggccg atagtaactc cgctttcatg tgcaacaagt
     7381 tgtgcagcat tggcgatcat atgggcacga ttgcccattc cttccttgaa ggtcttggta
     7441 ttaatcagaa tcagtggtga aggcatagtc attcctcatt catgtgcgaa taatttagtc
     7501 cctgatactc ctcttttata gttcagttat catgaatatt tgggcttgtt gctgattgtt
     7561 ggtatctgtt attctttcag gccgcgaata taatggccgg ctgtgattcc gccgagagca
     7621 tacagtttta cggtatcttt gaggagttcc aaccgcttat caggagtttt tatcccgtcg
     7681 tggacaaggg cgattgcttt tgtatagatg cccgtcatat ctcttgcata ccccgttgtc
     7741 cttccccgtg tgtcgatgat aacttcattg attcccatct gcaggagcga ggggagatga
     7801 tcgataaggc atagttctga ggaattgaaa atatgcgacc tgcactcgcc atccacgcgt
     7861 accggaaaaa tgtggcctgt tgaatcacgg ataccataga accggggatg atggcggtct
     7921 ttgtttttct gaacctcctt ttcccagggt tgcaggatgc aatcttctga aaccatggct
     7981 tcactgtttc cctggactat gagagaaaag gatgtaagac acccctggga tctcaccctt
     8041 cggatcagca gttccatttc atctcgcgac agttcaggag acagggtaac ggacctgaat
     8101 atcggggctg actgatacac agactggtga ttatagatat tgagcccgcc tgaaccgaac
     8161 agtccgatgt gcggagttgc ctgcatgagc gcgtaggcag taccgtaatt ttccactaaa
     8221 acgccggaga tattcagatc tgtgcccttt gagagaaacg gaaggacacc tttgaggtaa
     8281 ttgtttctgg taatgcgggg aaacttgagg acaaacctga ccccggcatc attgcacatc
     8341 gctgacaccg tgacaatctg tgcttcaaaa gaggaatgga cggaaatttt ccggcaggta
     8401 ctggtgcctg gtacgaacac cggctcaaag cagatgaagt cacacccgcc ttcgagggca
     8461 ccctgaacac cctcgattga atccgcatat acccctaacg ccagacgggc gggtgtgctg
     8521 ctgttccgcg ctgcaaggga tttggagaca ccatgcattt tgtgttcatt ccatcgacag
     8581 gatgactggt ctatgttctc ctgtgccggt cgggcccgtg ccacaatcat atcttcagcg
     8641 attttcagga actcccgccg cacataattc agccgggcta tcgtaagaaa ccagtttccc
     8701 tcagagtgaa cagtaaaatt gctgatggta aacggggtat ctttactctt ttttacatgc
     8761 aattcgatct gttcaggagt aaggggatga gtaagagccg gttcaagatt aaaatccggg
     8821 gaatacgtga cctggaattt ccccgcgatc cgggaatgaa caagaccttt tatctccagc
     8881 ctgcccaaag catcgatcgt cacgtctgca tcgacaagga ccgggtgccg caatccagga
     8941 gtcggatgag taataatccg gcgtgcccgc tgctcgaatg cgggagaaga tgtgataaaa
     9001 acccctgttc ccggttcaac aggttgtgga acccttaaag aaatttctcc gtctttttcc
     9061 accgggattg aattgaggga aaaaccgaac tcatgagagg gatttttttt gtgcctgaag
     9121 aggagtccgt ctcccctttg gggaattatt gattgtgtgg tacggatctt cacactcttt
     9181 gatttttcat cataccggtt caccacaccg atacaaaaac cccggttatc cggcgcatct
     9241 cttcccatca gatcggttcc gtgttttcca aaaagatatc ctaatgtcag accccggttg
     9301 aaagcaagac caagatcctg tagatcatca gtaacattga gagcttttcc gtctgcaacc
     9361 gaatcgatag cttttcggta cgtactgacg acgattgaaa cgtattccgg tgatttcatg
     9421 cggccttcta ttttcaggga aactaccggg gattttagga ggctgggaag tttttcgtac
     9481 gtgcacagat cttttgggga gagcgggtaa tgatcggtcg agggcagttc acgaattcct
     9541 gatggcctgc cataattatc tgtatctgca gttatcatcg agtagggttt acggcacggc
     9601 tgtgcacaca tgccacggtt cccgcttctt cccccgatca ctgatgaaag aagacattgt
     9661 cctgaataac aatagcagag cgccccatgc gcaaatactt caagtccgat accggattct
     9721 gcggttgcct gagaaatttc ctcaatttct taaatcgtca gttcccgtgc cagcacaacg
     9781 cgggaaaaac cctgttcagc tgcccactcg actcctgcgg cagaatggat agtcatctgt
     9841 gttgaggcat gaagaggtaa cagagggacg atttttctcg caagggcagc tattccgata
     9901 tcctggatga gaactgcatc tgttccgatg gaatacagcc agaggagatg ttccagcacg
     9961 tcatccagct cccggtcatg gatgagtgtg tttatcgtta catagacccg aacgtccctg
    10021 cgatgggcaa agtcaattgc ttcttttaac tcatcatcag taaaattc
//
LOCUS       DASRLV010000214         7304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_481784,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000214 DASRLV010000000
VERSION     DASRLV010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 7304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7304
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481784"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VFC43_08280"
     CDS             complement(<1..226)
                     /locus_tag="VFC43_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31208.1"
                     /translation="MLDMADLPDQARFCPGDQPMACYILPESLPVMTVDLSETPANGQ
                     VIPYLSRRWGIPIFGTLRKSIFTGAIRAVYR"
     gene            complement(420..491)
                     /locus_tag="VFC43_08285"
     tRNA            complement(420..491)
                     /locus_tag="VFC43_08285"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(456..458),aa:Arg,seq:gcg)
     gene            complement(677..1390)
                     /locus_tag="VFC43_08290"
     CDS             complement(677..1390)
                     /locus_tag="VFC43_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31209.1"
                     /translation="MTEIAARIICCITIMLVVSASGCVAPPKDNKTTSQTGNFFNPDQ
                     PTTVITQTPTYVSEVTAFITETTTSGYRTIPPTTLIPPDKYCRIYSTTNTYVYNKTAI
                     AFNLRSPPMFINYTVKPSNETRIKVIDSKLTGGGEQVLTIDTYSPFSWFEVTVRNKSG
                     GAIYLQDGFGKGKGYTEYLNRTLKVLKKDEMQIEFAGNNITATAMVWVKPDTNIDDTS
                     KFNLTTDCAYFDPNSRDFV"
     gene            complement(1730..1814)
                     /locus_tag="VFC43_08295"
     tRNA            complement(1730..1814)
                     /locus_tag="VFC43_08295"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1776..1778),aa:Leu,seq:cag)
     gene            complement(1856..3718)
                     /locus_tag="VFC43_08300"
     CDS             complement(1856..3718)
                     /locus_tag="VFC43_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZK31210.1"
                     /translation="MVRYSVTMDNELTTSIDKSCEKRKISRSEWINEACTTHLSTSSA
                     MNTVGATTLPSAGHVFGHNEHNMPDYPEMYRSFKIDVPEHFNFGFDIIDAWANKDRNK
                     LAMIWVDQEGKEKKYTFWDLMRLSNQIVNMMIKYGVNKGDRVLIMLPRVPEWWTFTLA
                     LIKRGAVYCPAPTMLTPKDLKYRINIADIKMVITSEEHADKIDEIAKDCPTLSCRMMI
                     DGKRAGWISYQVELDYPAPVSAKLVNLPGMKKTRSSDPMVIFFTSGTTGEPKMVLHDQ
                     SYPLGHIVTARFWQDVRTNDIHFTLSDTGWAKSAWGKLYGQWIEGAAIFVYDIRSKFN
                     PTEILPLIEKYGITTFCCPPTIYRMLILADLDKFDFTELRHCVSAGEPLNPEVIKAWK
                     DATGLTIFEGYGQTETVLCIGTFPGMTPKFGSMGKPSPGWHIELHNDAGKPVGLHEEG
                     RIAIRLNPRPVGLFCEYMNNEEENRKSFIDGFYYTGDKAYLDEDGYFWFIGRDDDVIK
                     ASGYRIGPFEVESALLEHPAVQEAAVVGSPDDIRGLIVKAFVILKPGTRPSEALVREI
                     QNHVKNVTAPYKYPRLIEFVDSLPKTISGKIKRNELRDRELKRSLNGNNAERRE"
     gene            complement(3842..5511)
                     /locus_tag="VFC43_08305"
                     /pseudo
     CDS             complement(3842..5511)
                     /locus_tag="VFC43_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329024.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            5675..6229
                     /locus_tag="VFC43_08310"
     CDS             5675..6229
                     /locus_tag="VFC43_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HZK31211.1"
                     /translation="MADKKYESLKIENIVASGVIADSIDLVDVSGKIKSCELNTKRFP
                     GAVYRIENPKIASLIFSSGKVVLTGIRDKKALTDGLRIIMKSLKDAGVDTYKEPKVAI
                     TNIVCSYDIGKYINLNKVVITLNLENIEYEPEQFPGLVYRIKDPKIVALLFSSGKIIL
                     TGGKNLEDIKKGLDFLEQKLESIM"
     gene            complement(6250..>7304)
                     /locus_tag="VFC43_08315"
     CDS             complement(6250..>7304)
                     /locus_tag="VFC43_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZK31212.1"
                     /translation="AEQDGIAFAEKFLHEMDKNRSLIPTDHLITLENTDDGVVCNVCA
                     GHKANEALGRVLSILISARYGTTVGLELDAYRMLLRLPSSIRASDVRDLLLSLDPLHM
                     PGIVRLALKRTVLFKWKLVQIAKKFGAIDPDADYEKISIQRLLEYFDGTVVQQEAYRE
                     LLSEYMDINAAIAIVELIVKGEIQVSLGPHSLIGAGGLLSSRDQIPPPTADKAVIETL
                     KRRLEQDDVLLVCMNCRDWKSRTVVSRVPDHPQCPKCGARLIAALKPYEDELYTLIRK
                     KSKTQEERVIDKRLLKNANIVLSSGRKAVVALSARGVGPENTSRILSTMAEGDDFYRE
                     IMKAERNFIQTHRYWS"
BASE COUNT         1955 a         1692 c         1665 g         1992 t
ORIGIN      
        1 gccggtaaac ggcccggatt gcccctgtaa agatactctt cctgagagtg ccaaaaatgg
       61 ggatccccca cctccggctg agatacggga tcacctgacc gttagcaggc gtttctgaca
      121 ggtctaccgt cattacagga agggattcag gcaggatata acatgccatc ggctgatctc
      181 caggacagaa tcgtgcctga tcaggaagat ctgccatgtc aagcatactt catatgacgg
      241 gaaacattgc tccagaaaat tttaaccgaa tcgcaccggt cgaaatttga caagttgaat
      301 gattaagaat aggtttggac attgaatgtg aagagcccat gccagcctta cattattcag
      361 ttaataattt ggaacagaat tggaatgatg gatataagat tttgacctga aaagagatac
      421 gccccggccg ggacttgaac ccgggtcaaa agatccgcaa tcttctagga tgtccactac
      481 cccaccggga cacaaaattt tggctgtctg ccttactaaa attgctttca tcgcataaat
      541 acctcaccgt ttaacgaatg agagaaaaat tcagtggcta aaaaccgatc gtatctgata
      601 aaaacaagaa aaatgatttg tttgatgtag ttcttttacc ctgttctcga gggtccgggt
      661 aatgccgtag ttctcactac acaaaatccc gggaatttgg gtcgaagtag gcgcaatctg
      721 ttgtcaaatt gaattttgat gtgtcatcaa tatttgtatc aggttttacc cataccatcg
      781 cagttgcggt aatattgtta ccagcaaatt caatctgcat ttcatccttc ttgagcactt
      841 tgagagtccg gttcagatac tcagtatacc ctttcccctt tccaaatcca tcctgaagat
      901 agatcgcccc gcccgattta ttcctgacgg tgacttcaaa ccatgaaaag ggactgtaag
      961 tatcaatcgt aaggacttgt tcacctcctc ccgtcaactt ggaatcgatg acttttatac
     1021 gggtttcatt ggacggtttc acggtgtaat taataaacat ggggggactt cgcaggttaa
     1081 acgcgattgc agtcttattg tatacgtatg tatttgtcgt cgaataaatg cggcaatatt
     1141 tatctggggg tatcagggtt gtgggaggaa tcgtgcggta cccactcgtc gttgtttcag
     1201 tgataaaagc cgttacttcg gaaacataag ttggtgtttg tgtaatcacc gtggtgggtt
     1261 gatcgggatt aaaaaaattc ccggtctgag aggttgtttt attgtctttt ggcggtgcaa
     1321 cacatccgga tgcggaaacc acaagcataa tagtgataca gcaaatgatc cgggctgcta
     1381 tctctgtcat acgttaatat tacagctatt catagtaatt aagtatattc ggatgtgtgt
     1441 ccgggcatag caaaaaagaa ggttcgtgtt actataaact tacctctgca aaagtttttt
     1501 ttgagaaacg aaaaattata atttccaatt atcgcgcact gataaggtca aatataattt
     1561 taacgcttaa tttgcctcgc tgtcaaaaaa tactaacaca caaacactcc ttatcgaaaa
     1621 ctttttatta cgtattatca gccaaaaaac aatgtaaagt tgagaaggta aaaacattat
     1681 agaattgcga aatgattgaa ttcattgaaa taaaccaaag acctggcaat gcgagggatg
     1741 ggattcgaac ccaagaactc ctgcgagatc aggccctgaa cctgacgccg ttgacctggc
     1801 ttggctaccc tcgcgcttgt atacattatg tatgaaaata aataaagata ggggatcact
     1861 cccgcctttc tgcattgttt ccattcagag aacgcttcag ttcacggtca cggagttcat
     1921 tccgtttgat ctttcctgaa atcgtcttgg ggagagagtc aacaaattca attaatcgtg
     1981 gatatttgta cggtgcagta acatttttca catggttctg gatctcacgg acaagagctt
     2041 ctgaaggtct tgtaccgggt ttgagaatca caaatgcctt aacaataagc ccacggatat
     2101 cgtctggtga cccgaccact gctgcttcct ggacggcggg atgttcaagc aaagcactct
     2161 caacctcaaa tggaccaatg cggtagcccg aggcctttat cacatcatca tctctcccga
     2221 tgaaccagaa atatccatcc tcatccagat acgccttgtc accggtgtaa taaaaaccgt
     2281 caataaaaga tttccggttc tcttcttcat tgttcatgta ttcacagaaa aggcctaccg
     2341 gccgggggtt aagccttatg gcaatccgcc cttcttcatg cagaccaacc ggttttccgg
     2401 catcattatg cagctcgata tgccagccgg gcgagggttt tcccattgac ccgaatttcg
     2461 gggtcattcc ggggaatgtc cctatgcaca aaacagtctc tgtctggcca tacccttcaa
     2521 agattgtcag cccggtggca tccttccacg ctttgataac ctcgggattg agcggctcac
     2581 cagcgctcac gcagtgccgc agttcggtaa agtcgaattt gtcaagatcc gctaagatga
     2641 gcatgcgata gattgtcgga ggacagcaga aagttgtgat gccgtacttc tcaattaaag
     2701 ggagaatttc cgtaggattg aatttgctcc tgatatcata gacaaaaatc gctgcacctt
     2761 cgatccattg tccatagagt ttaccccatg cacttttcgc ccaaccggta tcggaaaggg
     2821 tgaaatggat atcgttggtc ctgacatcct gccagaacct cgctgttaca atgtgcccga
     2881 gtggataact ctggtcatga agcaccattt ttggttcccc ggttgttccg gacgtgaaga
     2941 aaatcaccat tggatcggaa ctccttgtct ttttcatgcc gggaagattg acaagctttg
     3001 ccgaaacagg ggcaggataa tccagttcga cctggtaact gatccaccct gcccgtttac
     3061 cgtcaatcat cattctgcag ctaagtgtcg gacagtcctt ggcgatttca tcgattttat
     3121 ccgcatgctc ttcgcttgtt atgaccatct tgatatcagc aatattgatg cggtatttca
     3181 gatcctttgg agtgagcatg gtaggagcgg ggcaatacac tgcaccccgt ttgatcagcg
     3241 caagggtgaa cgtccaccat tcaggcactc ttggtagcat aataagcacg cgatcccctt
     3301 tgttgacacc atacttgatc atcatattga cgatctggtt ggaaaggcgc atcaggtccc
     3361 agaatgtgta ttttttctcc ttcccctcct ggtcaaccca gatcatcgca agtttgttcc
     3421 ggtccttatt tgcccacgcg tcgatgatat caaaaccaaa gttaaagtgc tcgggaacat
     3481 cgattttaaa agagcggtac atctcagggt aatcgggcat attgtgctca ttgtggccga
     3541 aaacatgacc agccgaagga agggtagttg caccaacggt attcattgct gagcttgtgc
     3601 tgaggtgtgt ggtgcacgcc tcattgatcc actccgaacg tgagattttt cgtttttcac
     3661 acgatttatc gatagaagtc gtcagctcat tgtccatcgt gacagaatat cgcaccattc
     3721 tagatcgatt tgatggtgca cataataggt ttttcgattg tattttgcac cagatttagc
     3781 cggctcacct gtaataatta gaattataac ccaaatgaag gaagaataaa gggtggtgca
     3841 gttactgcct ttcaatgtac tttttcaatt ctgatgctct caggtcgctt ctcctgattt
     3901 ttccggaaat tgttttggga agatcctgta caaattcaat ttcccgggga tatttgtagg
     3961 gtgccgttgt gcgctttacg tactttttga gttccctgct cagtgattcg gagggttcgt
     4021 atccgttatt cagaacaatg aacgctttga caatcatccc cctgattctg tccggagatc
     4081 ccacaactgc tgcttcccgc actgaggggt gttcaataag ggcgctttct acttcaaaag
     4141 gtccgatacg atacccggaa cttttaatca catcatcgtc cctcccgaca aaccagaagt
     4201 atccgtcggc gtcccgtagt actttatctc cggtataata ccagccgttt acgcatgttt
     4261 tcctgttctc ttcggcattg tcgatgtatt caacaaagag accagggggg cggggattta
     4321 aggaaacagc aagtcttcct tcctcgccat cacgaacggg caggccatta tcatcgtgca
     4381 cctgaatatt ccagcccgga gatggttttc ccatagatcc tgggcgggga tcaaggctgc
     4441 caaataaggc aacacaacag acggtctctg tctgtccgta accttcgcaa atcgtgagcc
     4501 ctgtgccctc tttccagaca cggattactt caggattcag gggttcaccg gcactacagc
     4561 aatggcgtaa tgaagacaga tcgaaacgat caagatctgc aagtatcagc atccggtata
     4621 tggtgggggg gcagcaaaaa gtggtgatct gatatttttc caggagggga agcacctcag
     4681 ttgcctgaaa tttcccggaa aaatggtaga caaagataca ggctccttca atccattgac
     4741 cgaaaatctt tccccaggcg cattttgccc agcccgcatc ggcaaccgta aagtgcaggt
     4801 cattatgatg aagatcctgc cagagtcgtg cagtgacaat atgtccgaga ggataactgt
     4861 gattatggag gaccatcttt ggttccccgg ttgtacccga agtgaaatag atcaacagag
     4921 gagccgtgct ctttgtccgc tgagtgtcgg gtatactcac ggaccggtgg gataccggtg
     4981 ccggatacaa caactcatac ggccagctcg cccacccttc gagctcagta tcaaccacca
     5041 gccggcaggt aagggtgggg cactgatcgc agatttcctc aacttttgag gtattttcaa
     5101 gatcggtaat aatcatgcgg aattttccag aattgaccct gtacgagata tccctcgcag
     5161 tcagcattgt cgggcagagg gggcaaagac cgcgcccagt ttagtcagtg caagggtaaa
     5221 gatccaccat tcaggaatac gcgggaggag gacgagaacc cggtcgcctt tgttgatccc
     5281 atacttaagc aggatatttg cggcctggtt tgagaggttt gagaggtcaa gaaaattgaa
     5341 tttcttctct tcgcttttct ggttcaccca aatcatggct agtttgttcc ggtctttttt
     5401 ggcccatctg tcaaccacgt cgaaaccgaa attgaaatac tcaggaacat caatggaaaa
     5461 atccctgcag gtcgtctcgt aatcagtaag ggtttcttct gatctggtca tgcaggtaat
     5521 tatcagtttt tttcctattt catttgactt gaaaatcaat ggtgatgaca cgaaaaaaac
     5581 aggaattaca gatgatcgtt tatgcgcaat aatgagaggg gacataaaga ttgtccatta
     5641 gtattatgat tcagggaaaa gcatagtata gtggatggca gataaaaagt acgaatcatt
     5701 aaaaattgag aatattgtag catccggtgt tattgctgat tccatcgatc ttgttgatgt
     5761 ttcgggtaaa attaaaagct gtgaacttaa cacgaagcga tttcccggtg ctgtctacag
     5821 gattgaaaat ccgaagatag cctcacttat tttttcttca ggaaaggttg ttctgaccgg
     5881 cattcgtgat aaaaaagcgc ttacggatgg gcttcgaata atcatgaagt ctctcaagga
     5941 tgccggtgta gatacctaca aagaacctaa agtagcaata acaaatattg tctgttcgta
     6001 tgacatcgga aaatacatca acctgaacaa agttgtcatc accctcaatc ttgaaaatat
     6061 tgagtacgag ccggagcagt tccccggcct tgtataccga atcaaggatc cgaaaatcgt
     6121 tgcccttctg ttctcttcgg gaaagatcat tcttacggga ggaaagaacc ttgaagatat
     6181 taaaaaaggc ctcgatttcc ttgagcagaa actcgagagt ataatgtaat acagcctttt
     6241 tttcagatat catgaccagt accggtgtgt ctggatgaaa tttctttcgg ctttcatgat
     6301 ctccctataa aaatcatcgc cttcagccat agttgacaga atacgggagg tattctccgg
     6361 cccgacaccc cgggccgaaa gggctaccac tgccttcctg ccacttgata ggacaatatt
     6421 ggcatttttt aagaggcgct tgtcgatcac gcgctcctcc tgagtcttgg actttttccg
     6481 gataagggta tacaactcat cttcataggg ttttagcgca gcaataagcc gtgcaccgca
     6541 ttttggacat tgcgggtgat cgggcacacg ggaaacaaca gtcctgctct tccagtcacg
     6601 gcagttcatg cacacgagca gtacatcgtc ctgctccagc cttcgtttca gcgtttcaat
     6661 caccgcttta tccgccgtag gcggtgggat ctgatcacgg gatgagagca gacccccggc
     6721 accgatcagc gaatgagggc caagcgaaac ctgaatttct cccttaacaa tcaattcgac
     6781 gatagcgatt gcagcattaa tatccatata ttccgaaagc aattcccggt atgcttcctg
     6841 ttgtacaacc gtgccatcaa aatattcgag caggcgttgg atgctaatct tttcataatc
     6901 ggcatccggg tcgatcgcac caaacttctt tgctatctga accagtttcc acttgaaaag
     6961 tacggtacgt ttcagcgcaa gccggacaat tcccggcata tgtaacgggt cgagagagag
     7021 gaggagatcc cgcacatctg aagccctgat cgaagaaggc aacctcagga gcatgcgata
     7081 tgcatcaagt tcaagaccca cggttgttcc ataccgtgct gatatcagga tagatagtac
     7141 tcgtcccagt gcttcgttcg ccttatgacc tgcacagaca ttacagacca ccccatcatc
     7201 agtgttttcc agtgtgatga gatgatcagt gggaatcagg gaacggtttt tgtccatctc
     7261 gtggaggaat ttctctgcaa aggcaatgcc atcctgctct gcgg
//
LOCUS       DASRLV010000215         7126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_644224,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000215 DASRLV010000000
VERSION     DASRLV010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 7126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7126
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644224"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VFC43_08320"
     CDS             complement(<1..291)
                     /locus_tag="VFC43_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31213.1"
                     /translation="MSVFLHLPLHGNTVRPSHKGCIAWLHDGQLRGEGCCKALEGLVT
                     RQPGRCRDKRDRGLIFRHWKTLTPSALIASPGHDRTRRRQFPQNSTASGVFEG"
     gene            complement(366..1898)
                     /gene="lysS"
                     /locus_tag="VFC43_08325"
     CDS             complement(366..1898)
                     /gene="lysS"
                     /locus_tag="VFC43_08325"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HZK31214.1"
                     /translation="MTSPTENTAFDQTRLDKIRALQDKGITIFPSTFDRQHTVHDIKT
                     RYADITHDKSADPVSTAGRIYIVRNHGKTIFADIGDESGKIQLYIRKNDLGEEQFELF
                     NQFIERGDIIGVTGHVFRTKLGEITIWVDSITLLCKAVCSLPEKFHGLTDIEKRYRQR
                     YVDLIVNEESRQTFRNRSRIVSLIRRYLDDHGFLEFETPILQPVYGGANARPFTSYHN
                     FLDQKLFLRIAPELYLKRLVVGGFEKVFEIARNFRNEAVDTDHNPEFSMVEIYWAYQD
                     FRDMMTLTEDIISSIVFEVHGKYEIPYGETILDFKAPWKKLSMADSVWEVAGIDIFTH
                     TVEDLKKLAQQHRLEDWEKPRTQREFLVAFFEGLVEEKLIQPTFIYDYPVENSPLAKR
                     HRTKEGFTERFELFISGMEVGNGFSELNDPVDQKARFEDQDEKRRLGDIEAQMIDYDF
                     INAIGYGMPPTGGVGIGIDRLVMLLTNNSSIKEVILFPSMKSVQAEEGDEKDQGTDEE
                     SG"
     gene            complement(2036..3370)
                     /locus_tag="VFC43_08330"
     CDS             complement(2036..3370)
                     /locus_tag="VFC43_08330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZK31215.1"
                     /translation="MIVVLGGGPGGRIASIRLASAGKEVRLIEHGGIGGQCLHFGCMP
                     VCALNDVARTIHSIRIFQHLSLFDSIPQINFLRMLDEMRSIQTKIASVLDAETKSAGV
                     EITYGKTGRLEGKTVFVADEMIAADKVIAATGSFPLIPDIKGTNLPGVYTPHNIFQLK
                     TMPKKLTIIGGGVMAAEFAFIFSTLGSEVTLLSRSAFLKNIDSHLRTLAKKDLAPVNI
                     LENTHVQSIDGGFRVQSLQIVTEGQISHSEADAVLIATGLVPRSDMLNGLKKGPAGEV
                     IVDEHMQTSVPDVYACGDVVGPPYLTPVARYQGIVAADNILGIPRKMDCRFIPQSINL
                     SQELAFCITKNEDMASLAMPGPAGPGTFWSVPAGNTGLAKIIIEPDTGQLRGVCAAGP
                     GGGLITGYIAFLMRHHFSVKDFEHFIEVHPSTDGVYWLAKYAAERLENQKKP"
     gene            complement(3464..4432)
                     /locus_tag="VFC43_08335"
     CDS             complement(3464..4432)
                     /locus_tag="VFC43_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31216.1"
                     /translation="MMEHDMREKAKHAMRLILEAARFEVEEVDDPLDLSAVRDETCLL
                     VLCSNDSDLISQFDKTNYSLMIDDHEMTCKKLLFSIDRDIVTENCIHWGVDEFVRYTG
                     EAVLADILDRELALDLTPSKGKKIPVSAASAGKEEPRKEEASGITIPHYPVKITEQAA
                     MRTGGVQGTAKLRFIPYYLFHYTSSGEQVYKDRRIPFDADGWGAINAINGIKIDLDDK
                     LVEEKEIPGGAEVSDPHIRKEEATERIVNELIDRLTQKVRIKQEKGDAIFYEEKILKP
                     EKKNISVDARQVYIPIWQIRGKKIVEVNAFTGELLSEPMDEGVEIF"
     gene            4475..5248
                     /locus_tag="VFC43_08340"
     CDS             4475..5248
                     /locus_tag="VFC43_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF128 domain-containing protein"
                     /protein_id="HZK31217.1"
                     /translation="MSEITIMTVPFVPLKFVNHSIEDFAMQVTFDPASGDGRVVYNLS
                     VIKNEDLEFAVSTLKDAYKTGVTVSGRVKFLSSGDKMPGYVVPKGFTGICTICSITFD
                     GILIRRGIPITPIGGGVVEIEKRTPMRFTHIILYEHTTIDPLQVLISQKTTAITSVMR
                     TGSGSILANIREFHMEAEHQVGTVLDELSTSSLTGILEVGIPNLPLLGVPVSPQFVAI
                     AAVGGTNPMAAIREGGRWVQTQAMKGLMDISQMDEIRDY"
     gene            complement(5260..6045)
                     /locus_tag="VFC43_08345"
     CDS             complement(5260..6045)
                     /locus_tag="VFC43_08345"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZK31218.1"
                     /translation="MKLPSENPVRVIECLKSLGLTKYEALVYIALLKLVSATASEIHE
                     ISGVPRASVYTVIDQLLDKGLVSVSLSAPKRFAAIPPEDSITRLMDLIEKDAQYARDT
                     LSLVYRERMTPGLGTGELIWNVYGIENIKKRFADLISNAKQEIRIIAHPQFLSPDIQQ
                     ILTTTANQISVEIITPHWAGETPKKMKVYAIKHPEIPKELDMAKDMMAGGVCIIDDRS
                     VIVLLGVGEENAVALFSESDGFVRFFVRYYNLIFDWARKTKVK"
     gene            6111..>7126
                     /locus_tag="VFC43_08350"
     CDS             6111..>7126
                     /locus_tag="VFC43_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HZK31219.1"
                     /translation="MNYYQIIIVFLALGLWITTPVIGVTTYLGGSPQMSAALSGTNEF
                     TPGQDATIQVVVWNSGVNDFKFVTKGTIEQDDLPTTAKMVTIGLAAGNSPVIIKTDPQ
                     VVGDIKSQGIANVAIATKITSDATFGEYQLPLTISYTYLSSSDQEAADVLRFNYKQVN
                     ETIPITIKIKHQVKIEVLEAVPENLNVGTEGYLNLTIKNIGLEDGRKASVKILRNGAS
                     PIIPSDSSVFIGDFPHNSTVTCRYKVAVSDEAEIQSYPVDVLVMYENREGDVVSSTAD
                     TIGIPVWGKISFIITSGTVRVLQGSQSVIQIVYKNTGTRPAYHAQARLSAVEPFKSSD
                     DTAYL"
BASE COUNT         1736 a         1774 c         1776 g         1840 t
ORIGIN      
        1 cccctcaaaa accccgctgg ctgtggaatt ctgtgggaac tgcctgcggc gtgtgcggtc
       61 atgcccgggc gaggcaatca gtgccgatgg agttaatgtc ttccagtgcc ggaatatcag
      121 cccccggtcc cgtttgtccc ggcagcgacc tggctgccgg gtcaccagtc cctccagcgc
      181 cttgcagcaa ccctcgcccc tgagttgccc gtcatgcaac catgcgatgc agcctttgtg
      241 agacggtctg accgtgtttc catgaagggg aagatgcaaa aaaaccgaca aggacgtgat
      301 ccttcccgag tatgattgga agtaacaaaa aatgtgtata tgaatgattg tcaggagaac
      361 aggaattacc cgctttcctc atcggtaccc tggtcctttt catccccttc ttcggcctgc
      421 acgcttttca tggaggggaa caggataacc tccttgattg aactattatt cgtcagaagc
      481 atcacgagcc ggtcgatacc tatgcccaca ccaccagtcg gtggcatgcc ataacctatg
      541 gcattgatga aatcgtaatc gatcatctgt gcctcgatat caccaagccg ccgcttctcg
      601 tcctggtcct cgaacctggc cttctggtcg accgggtcgt tgagctccga gaagccgttg
      661 cccacttcca tgccgctgat gaagagctcg aaccgttcag tgaaaccttc tttggtgcgg
      721 tggcgtttcg cgagtggcga gttctcgacc gggtaatcgt agatgaaggt cggctggatc
      781 agcttctcct ccacaaggcc ctcaaaaaat gccacgagga actcccgctg ggtcctgggt
      841 ttctcccagt cctcgagccg gtgctgctgt gcgagcttct taaggtcttc cactgtatgg
      901 gtaaaaatat cgatgccggc aacttcccac acagagtccg ccatcgagag tttcttccat
      961 ggtgccttga agtccaggat ggtctcaccg taagggatct cgtatttacc gtggacctcg
     1021 aagacgatag atgagatgat gtcttcggtc agggtcatca tgtcacggaa atcctggtat
     1081 gcccagtaga tctccaccat cgagaactcg gggttgtggt cggtgtccac tgcttcgttc
     1141 cggaaattcc tggcgatctc gaagaccttc tcgaaccccc caacaacgag gcgttttaaa
     1201 tagagttccg gggcaatcct gaggaagagt ttctggtcaa ggaagttatg gtaagaggtg
     1261 aatggccggg cattggcacc cccgtatacc ggctggagaa tgggggtttc gaactcgagg
     1321 aacccgtggt catcgaggta ccggcggata agggatacga tccggctccg gttccggaag
     1381 gtctgcctgc tctcctcgtt gacgataagg tcgacgtacc gctggcggta gcgcttctct
     1441 atatcggtaa gtccgtggaa cttctcgggc agggagcaga ccgccttgca caggagggta
     1501 atcgaatcta cccagatcgt gatctcgccg agttttgtcc ggaaaacatg gccggtcacc
     1561 ccaatgatgt ccccccgctc gatgaactgg ttgaagagct cgaactgctc ctcgccgaga
     1621 tcattcttac ggatatagag ctggatcttg ccactctcgt ccccgatgtc agcaaagatg
     1681 gtcttgccat ggttacggac aatgtagatc ctgccggcag ttgagacagg atcagcactc
     1741 ttgtcatggg tgatatctgc ataccgggtc ttgatatcgt gcaccgtgtg ctgccggtca
     1801 aacgtggatg ggaagatcgt gatacctttg tcctggagtg cacggatctt gtcaagccgt
     1861 gtctggtcaa aagctgtatt ttcggttgga ctggtcatcg taaaaacacc cgggggctgg
     1921 gatgatgtgt atcgtaataa tgctattttc gatctaataa taccttcagc atcagcgccg
     1981 gtatacctcc ttccttgttc agggtaattt cagcagaaaa caaattctct gttggttagg
     2041 gctttttttg gttttccagc ctttcagcag catattttgc cagccagtaa acgccatccg
     2101 ttgaagggtg gacctcgata aaatgttcga agtccttgac tgaaaaatga tgtctcatga
     2161 gaaacgcgat gtaaccggtg atcaggccac ccccgggtcc tgcagcacat acacctctga
     2221 gctgaccggt gtccggctcg atgataatct ttgcaagacc ggtattaccc gctggcacag
     2281 accagaatgt tcccgggccg gccggccccg gcatcgcaag agacgccata tcctcatttt
     2341 ttgtgatgca gaatgcaagt tcctgtgaga ggttgatcga ttgagggatg aaacggcaat
     2401 ccatcttccg ggggataccg aggatattat ctgcagcaac tataccctgg taccgggcta
     2461 ctggtgtaag gtagggaggt ccgaccacat ccccgcatgc gtacacatcc ggcacactgg
     2521 tctgcatatg ctcgtcaaca atgacctccc cggcaggtcc ttttttaaga ccgttcagca
     2581 tatcagaacg cggtacgagt ccggtcgcaa tcagtacggc atcagcttca ctatgggaga
     2641 tttgtccttc tgtcacaatc tggagagatt gtacccgaaa acccccatca attgactgaa
     2701 catgggtatt ttccaggata ttcaccggtg caagatcctt ttttgccaga gtgcggaggt
     2761 gtgaatcgat attttttaaa aatgcgctcc gggacaggag ggtgacctcg cttcccagcg
     2821 tgctgaaaat gaacgcaaat tctgcagcca tcacacctcc cccgatgatt gtaagtttct
     2881 tgggcatcgt tttgagctgg aatatattgt ggggggtgta cactcccggc agattagtcc
     2941 ctttgatatc aggaataagg ggaaaggatc cggtagctgc aataacctta tccgccgcaa
     3001 tcatctcgtc tgctacaaaa acggtttttc cctcaagcct gccggttttt ccataggtaa
     3061 tctccacacc ggcggatttt gtttctgcgt cgagcacaga tgcgatcttt gtctggatag
     3121 agcgcatctc atcgagcatc ctgagaaaat tgatttgcgg aattgaatca aaaaggctga
     3181 ggtgctgaaa tatcctaatg gaatggatcg tgcgggcgac atcattgagg gcacaaaccg
     3241 gcatgcaacc aaaatggagg cattgaccgc caatcccccc atgctcgatc agccggactt
     3301 ctttacctgc agaagcaagc cggattgatg cgatccttcc acccgggccg cccccaagaa
     3361 ccacgatcat gggtattcct ttgtcatgga cagagaaatt ttgatccttt tagtgagaaa
     3421 agcaggacgt ctcctgtttt cttagtcctg tgcaaaaggc tcgttagaat atttcgaccc
     3481 cttcgtccat cggttctgag agcagctcgc cggtaaacgc attaacctcg acgatctttt
     3541 tgccccgaat ctgccaaata gggatataga cctgccgggc atcaacagag atatttttct
     3601 tttcgggttt gaggatcttc tcttcgtaga agatcgcgtc tcctttctcc tgcttgatgc
     3661 gtaccttctg ggtaagccgg tcaatcagtt cattgacaat ccgttcggtt gcctcctcct
     3721 tccggatatg gggatcagaa acttcagcgc ctccggggat ctctttttcc tcaaccaact
     3781 tatcatcaag gtcgatcttt atcccgttga ttgcattaat tgcaccccat ccgtcggcat
     3841 caaagggaat gcgccggtcc ttgtagacct gttcgccact actcgtgtaa tgaaaaagat
     3901 aatacggtat gaaccggagt tttgcggttc cctgaactcc cccggttcgc attgctgcct
     3961 gttcggtgat ttttaccggg taatgcggga tggtgatgcc agaggcctct tctttccggg
     4021 gctcttcctt cccggcagat gcagcagaaa caggtatttt ttttcccttc gatggtgtga
     4081 gatccaaggc aagttcccgg tcgaggatgt cggcgagtac ggcctccccg gtgtaccgca
     4141 caaattcgtc gacaccccaa tggatacagt tttctgtcac aatatccctg tcaatagaaa
     4201 aaaggagctt cttgcaggtc atctcatgat cgtctatcat gagactgtaa tttgttttgt
     4261 cgaactggct gatcaggtca ctgtcatttg aacagagcac caggaggcag gtttcgtcac
     4321 gtaccgcaga aaggtcaagg gggtcatcaa cttcttcgac ctcaaaacgg gctgcctcaa
     4381 gaatgagccg cattgcatgt ttggcttttt cccgcatgtc gtgttccatc atacataaat
     4441 tgccatggtc ttatagaaca atttttctat tgcgatgtct gaaattacta taatgactgt
     4501 cccgtttgtc ccgctcaaat tcgtcaacca cagcattgaa gattttgcaa tgcaggtgac
     4561 ttttgatccg gcctcgggtg atggcagagt tgtgtataac ctgtccgtca taaaaaacga
     4621 agacctcgaa tttgcagtct ccacccttaa agatgcgtac aagaccgggg ttaccgtaag
     4681 tggacgtgtc aaattccttt cttccggcga taaaatgccc gggtacgtgg tcccaaaagg
     4741 attcaccggt atatgtacaa tatgcagcat tacctttgat ggaatcctga ttcggagggg
     4801 aattcccatc actcctatcg ggggcggggt tgtcgagata gaaaaacgaa cccccatgcg
     4861 gtttacccac atcatcctgt atgaacacac gacaatcgat cctcttcagg tgctgatctc
     4921 ccagaagacg actgctatca ccagtgttat gagaacggga agcgggtcaa ttcttgcaaa
     4981 tatcagggaa tttcacatgg aagctgaaca ccaggtcggt actgtgctgg atgagctctc
     5041 aaccagcagt ctcaccggga tactcgaagt tgggatcccg aatcttccac tccttggtgt
     5101 acccgtgagc ccgcagttcg tcgctatcgc ggcagtgggt gggacaaatc cgatggctgc
     5161 aatacgagaa ggtggtcgct gggtacagac acaggcaatg aagggactga tggatatctc
     5221 gcagatggac gaaatcagag attattaatc cccagtctct tattttacct tggtttttct
     5281 ggcccagtcg aagatcaggt tgtagtaacg cacaaagaaa cggacaaagc catcggattc
     5341 tgaaaaaagt gctactgcat tttcctctcc cactcccaat agaacgatta cactacgatc
     5401 gtcgatgatg cagactccgc ctgccatcat atcctttgcc atatcaagct cttttgggat
     5461 ttcgggatgc tttatggcat atactttcat ttttttggga gtctctcctg cccagtgggg
     5521 ggtgataatt tcaacactga tttgatttgc cgtagtggta aggatctgtt gaatatcagg
     5581 agataaaaac tgggggtgag caataattct gatctcctgc ttcgcattgg aaatgagatc
     5641 cgcgaaccgt tttttgatat tttctatacc gtagacattc cagataagct ccccggttcc
     5701 aagtcccggg gtcatgcgtt cccgatatac caatgataag gtatcacgtg catattgcgc
     5761 atctttttcg attaagtcca ttagtctggt aatggagtct tccggaggaa tggcagcaaa
     5821 ccgtttgggt gccgacagtg atacagagac cagtccttta tcgagaagct gatcaattac
     5881 agtatagacc gacgcgcgag gaaccccgga tatttcatga atctcgcttg ccgtagcact
     5941 aaccagcttc aaaagagcaa tgtatacgag tgcttcatat tttgtcagac cgagtgattt
     6001 tagacattca ataacccgga caggattttc tgacggcaat ttcattgaaa catcacttta
     6061 tatcctgctg taataaatgt tgttataaca ataacaacaa gtgatgtctc atgaattatt
     6121 accagataat aattgtgttt cttgcgctgg ggctctggat cacaacgccg gttattggtg
     6181 taacgacata tcttggcgga agtccccaga tgtcggcagc actatcgggt accaacgagt
     6241 ttaccccagg gcaggacgct actattcagg tggtcgtgtg gaatagtggg gtgaatgatt
     6301 tcaaattcgt aacaaaagga acgatcgaac aggatgacct gcccacaact gcaaagatgg
     6361 taactatcgg attagcagcc gggaattctc cggttattat taaaaccgac ccgcaggttg
     6421 tcggggatat taaaagtcag ggtatcgcaa acgtcgcaat tgcaacaaaa ataacttcag
     6481 atgccacgtt tggggagtat cagttgcctc ttaccattag ttatacgtat ctctcctcct
     6541 cagatcagga agctgcagat gttctccggt ttaattataa gcaggtgaat gaaaccatcc
     6601 cgataacaat aaaaatcaaa catcaggtga agattgaagt cctcgaagct gtgccggaaa
     6661 atctgaatgt cgggactgag gggtacctga acctcacgat caaaaatatc gggctggagg
     6721 atggcagaaa ggcgtccgta aagatacttc ggaatggagc aagtccgata attccatcgg
     6781 acagcagtgt ttttatcggg gatttccccc acaacagcac ggttacctgc agatacaagg
     6841 tagcggtctc ggatgaagcg gaaatacagt catatcccgt tgatgtcctt gtcatgtatg
     6901 agaaccgcga gggagatgtc gtgagctcaa ctgcggatac tattggtatt cctgtctggg
     6961 gaaaaatcag tttcattatc acctccggaa cagttcgggt acttcaggga tcacaaagtg
     7021 tgatccagat cgtctacaaa aacaccggaa ccaggccggc ataccatgca caagcgcgcc
     7081 tgtcagccgt agagccgttt aaaagcagcg acgatactgc atatct
//
LOCUS       DASRLV010000216         2502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_482532,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000216 DASRLV010000000
VERSION     DASRLV010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482532"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="VFC43_08355"
     CDS             complement(<1..1019)
                     /locus_tag="VFC43_08355"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HZK31220.1"
                     /translation="MQETQINPWSSTPSLDIEKTFTDFGIDPIAPVISELPEIPYFMR
                     RGIVIGHRDYRPIALAIRNKTPFHILTGFMPSGHPHLGHLMVMKEVVWHVQQGGNGYI
                     TIADREAHAVRGLSWEKCNEYGKEYLSCLCALGFEGQTYFQSKNNRLKDLAFEAATKV
                     NFSELTAIYGFGADTELAHADSVITQVADILYPQIDREPAPTVVPVGVDQDPHIRLTR
                     GIAHKMRMFTVEERDGYISVRSKNAPDLALEAVKKAFPHAKKYEGHVDIRDAQCADVA
                     AKVRKIERAHGGFAFFTPSSTYHIFMPGLTGGKMSSSIPESIISFFEPEATVRKKVMS
                     GITGG"
     gene            complement(1029..2039)
                     /gene="endA"
                     /locus_tag="VFC43_08360"
     CDS             complement(1029..2039)
                     /gene="endA"
                     /locus_tag="VFC43_08360"
                     /EC_number="4.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000214 - tRNA-intron endonuclease
                     complex [Evidence IEA];
                     GO_function: GO:0000213 - tRNA-intron endonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-intron lyase"
                     /protein_id="HZK31221.1"
                     /translation="MKASFDGTVVRVGKEGRVLYDQSGYGRLEQEGVKLSDQEALYLL
                     HRHKIEITGYTFNSLFSDFAKRPGFLRSFLVYRDLRERGYVVQAWPHDFRVFKRGEKP
                     GTGESLYLVRVLSERDPIRFVKLIEEVVASRNMRKQYVLAVVDDEEELTYYEIKLQRL
                     AEPDNNTPDVKMNEAILVGKTGMVLAAPQSDLERLGFGKRLDDERLMLAPVELLYLMG
                     KGIIELRKDSIPVRQDEYFTLAGDADSELAEKDKVYTELRGHNFTPRTGYKFGHHFRV
                     YCGRNVHSDMLVHAVEKETSLPMSAISRSVRMAHSVKKKMLFGAVHTKGIQFVEFARI
                     KL"
BASE COUNT          598 a          673 c          618 g          613 t
ORIGIN      
        1 ctgccaccgg taatcccgct catcactttc tttctcaccg ttgcctcggg ctcgaaaaag
       61 gatatgatgc tctccgggat gctgctcgac atcttgccac ccgtgagacc cggcatgaaa
      121 atgtggtatg tggatgaggg ggtgaagaat gcaaaacctc catgcgcccg ctcgatcttc
      181 cggacttttg cggcaacatc agcacactgc gcatcacgga tatcaacgtg tccctcatat
      241 ttcttcgcgt gcgggaacgc tttttttacc gcctcaagtg ccagatccgg cgcgttcttg
      301 gagcggacgc tgatataccc gtcccgctcc tctacggtaa acattcgcat cttgtgggct
      361 atcccgcgag tgagccggat atgcggatcc tgatcaactc cgaccggtac aactgtcggc
      421 gccggctcgc gatcgatctg cgggtagagt atgtcagcca cctgggtaat gacactgtct
      481 gcgtgggcaa gttctgtgtc agcgccgaat ccgtagatcg ctgtgagctc cgagaagttg
      541 accttggtcg ctgcctcaaa ggcaagatcc ttgagccggt tattcttgct ctggaagtag
      601 gtctgtcctt caaacccgag tgcacagaga caggagaggt actccttccc gtactcattg
      661 catttctccc acgagagacc cctcaccgca tgggcttccc ggtcggcaat agtaatgtac
      721 ccattgccac cctgctgaac gtgccagaca acctccttca ttaccatcag atgcccaagg
      781 tgaggatggc cgcttggcat gaacccggtc aatatatgga agggcgtttt gttcctgatc
      841 gccagagcaa tcgggcggta gtcccggtgc ccgattacaa tgccacggcg catgaagtac
      901 gggatctccg gcaattcgga tattaccggt gcaatggggt cgataccaaa atcggtaaac
      961 gtcttctcga tatcaaggga gggggtgctt gaccagggat ttatctgcgt ttcctgcatg
     1021 gtgtgtgctc aaagtttgat tcgtgcaaat tcaacgaact gaattccttt agtatgtaca
     1081 gcgccaaaca acatcttctt tttgacactg tgcgccatcc ggacggaccg cgaaatcgca
     1141 ctcatcggaa gagacgtctc tttttcgaca gcatgcacca gcatatccga atgaacattc
     1201 ctgccgcagt aaaccctgaa atggtggccg aacttgtaac ctgtccttgg ggtaaaatta
     1261 tgccccctga gttcggtata gaccttatcc ttctctgcaa gttcgctatc ggcgtcaccg
     1321 gcaagtgtaa aatattcatc ctggcgtacc ggaatgctat cttttctgag ttcaataatt
     1381 ccttttccca tgagatacaa gagttcaacg ggagcgagca tgagccgttc atcgtcaaga
     1441 cgtttaccga atccaaggcg ttcaagatct gactgagggg cagccagcac catacctgtt
     1501 tttcccacca gaatcgcttc attcatcttt acatcaggtg tattattgtc gggttctgcc
     1561 agcctctgaa gtttaatttc ataataggtc agttcttcct cgtcatcaac cacggcaaga
     1621 acatactgtt ttcgcatgtt tcttgaagca acgacctcct cgataagttt aacaaacctg
     1681 attgggtcac gttctgacaa caccctgaca aggtataacg actctccggt accaggtttt
     1741 tcaccacgtt taaagacacg gaaatcatga ggccatgcct ggacaacata accccgttcc
     1801 cgcaaatcac ggtataccag aaaactgcga agaaaccccg ggcgtttggc aaaatctgaa
     1861 aaaagggagt tgaaggtgta accagtaatc tcgattttat gacggtgaag cagataaagg
     1921 gcttcctgat cagagagttt cactccctct tgttccagcc gcccgtaccc actctgatca
     1981 tagagtaccc gcccctcttt tcccacacgc accacggtgc cgtcaaatga tgctttcact
     2041 accggatata ttgttgttgg gggattataa gggcgcgggg aagggaagaa tactggttgg
     2101 gatccaacct gcaatgagca ccgttaccgg tttctcaccc gtaacggtgc gcgtatccaa
     2161 gcgccggcca ggtcgcccgt atcgttatca gcgcgtcata taattcccgg tcccggaggg
     2221 gaatacccat ctatgaaatc ggcgatggga gcggtgatgc taaccagtgc actattgagc
     2281 tcatatccga ttccagtggg gggatgcgaa tagccgggcc agcgccggat gctgaaacag
     2341 gaagaatacc cgtgtgggta aatgaacaca caaccacagt ccttgtcgca tcgcctccgg
     2401 cacagaaagg tgaaccgcgt ttcgaactca cgttcactat tgacaggaga aggaatcttt
     2461 gtgtcactgc gcgagatatc ataacgggta tgctggttaa aa
//
LOCUS       DASRLV010000217         4238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_968084,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000217 DASRLV010000000
VERSION     DASRLV010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968084"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..353)
                     /locus_tag="VFC43_08365"
     CDS             complement(168..353)
                     /locus_tag="VFC43_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31222.1"
                     /translation="MHPEHNRNRIRCGLGQGLHPVLCLHPLVPDGAIEIGGRTKGKPR
                     YHHPDITPRDMLDQQGE"
     gene            complement(434..982)
                     /locus_tag="VFC43_08370"
     CDS             complement(434..982)
                     /locus_tag="VFC43_08370"
                     /inference="COORDINATES: protein motif:HMM:NF024055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HZK31223.1"
                     /translation="MEKNGIDTGNPKVLRSLVIVFSYHHNNTEKIANACAKVLGAEVK
                     TPQQVKPEEIAEYDLIGFGSGIYSATFDPSVLDLADRLPDAAEKKAFLFSTYGAPAVF
                     AGGDFVKNNHSQIREKLKAKGYTVIGEFGCAGWNTNSFLKYFGGLNKGRPNTEDLKNA
                     EAFAREMKEKSRKQEAEHERKG"
     gene            complement(1002..1715)
                     /locus_tag="VFC43_08375"
     CDS             complement(1002..1715)
                     /locus_tag="VFC43_08375"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HZK31224.1"
                     /translation="MKTDDTNKKTAIIVGVLYIIGTIAGMLSYVFTGPVLGDPDYLVN
                     VSAHEMQIITGALCVLIMGLALAMVPVMMFPILKKYHEALALGYVVFRGALETVMYII
                     LAIVWLFLIPLSQEYVKAGTPDASYFQTLGAVVLKVHDLIGPVQTIVFCLGALIFYYL
                     LYQTNLIPRWLSGWGLIATIPYLAAGLLVLFGFFGPLSTSEIVLEFPLALQEMVMAVW
                     LIVKGFNLSSIASGSATGE"
     gene            complement(1761..2483)
                     /locus_tag="VFC43_08380"
     CDS             complement(1761..2483)
                     /locus_tag="VFC43_08380"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HZK31225.1"
                     /translation="MTFPTSDISRNKTARIAGILWFIIIVTGIFAEFFVRAGLIVPGD
                     AATTAGNILANEWLFRVGIVSDLFMGACFVLLAWTLYILLKPVNKDIALLFVVIAAIS
                     ITIQCINMVNQVAALQILTNPGLAQAFSTGQLHALMMFFLDMHRYGYTIDAIFYGGWL
                     FPLGYLVYQSGFLPKISGILLMAACIGDWIGVAQVFLFPGFEVITYPGFLVAVIAEFT
                     FCGWLMVKGVKESEQVLPEASS"
     gene            complement(2494..2937)
                     /locus_tag="VFC43_08385"
     CDS             complement(2494..2937)
                     /locus_tag="VFC43_08385"
                     /inference="COORDINATES: protein motif:HMM:NF039408.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6326 family protein"
                     /protein_id="HZK31226.1"
                     /translation="MTNLTPGILEDVKIHVKLKISALWIAVMLLFAYGDIFSFFRTGL
                     IEGIIAGKISGFQINQGFLVGSSLYIAIPCLMVFLSLVLRPDVNRWANIVLGLLYTAT
                     ILLFCIGEEWAYYFFLSILESVLLLVIVWYAWKWPEQESPMKSVT"
     gene            complement(2968..3693)
                     /locus_tag="VFC43_08390"
     CDS             complement(2968..3693)
                     /locus_tag="VFC43_08390"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HZK31227.1"
                     /translation="MTTYDTNRKTAIIAGTLLIIATFASILSNLFLKSVHSSDYLVEI
                     SANGNQITAGALLILIAAVTSASIAISLYPVLKKYSEGLALGAVCFRLIEGVFYIVGI
                     LGLVLLLTLSQEFVQAGMPDASFFQILGTLLLAGREWAGFGIGPTAFGLGSLMYYSIF
                     YQTELIPRWLSGWGLAGAVLCMVASVSVMSGLISPLSTIHIVLNLPIALQEMVLAVWL
                     IVKGFNPSAIASLSLRTDINEVK"
     gene            complement(3921..>4238)
                     /locus_tag="VFC43_08395"
     CDS             complement(3921..>4238)
                     /locus_tag="VFC43_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31228.1"
                     /translation="VWVGSLLIGLIVGYCVSYILIRYFDAIPSKNPILKSGIISFIAL
                     IIAIILIDVPQSFFGMSNSSAALYYFLIGVMFNGARFLLLGIVIGCLYTEPAHSLFTG
                     DKK"
BASE COUNT         1175 a         1051 c          947 g         1065 t
ORIGIN      
        1 aagttaatgt tctggcctat tgttggcggg gttactcttt tttttggata ttttgacggg
       61 atcaagggtg cctttgtggg tttcctgtat ggagtggcgg gaggactgtt tcttctccac
      121 gaacggtcgc ggtgcaataa aaagtgagaa tacggtcgca tccagggtta ttcgccctgc
      181 tggtccagca tgtcccgcgg agtgatatca gggtggtggt accgcggctt gcctttcgtc
      241 ctgccaccga tctcgatcgc tccgtcgggc accagtggat gcaggcatag cactgggtgc
      301 agtccctgtc ccagcccaca ccggattcgg ttacggttat gttccgggtg cataccagaa
      361 ttcttttaca gatttcccgt atgtcgcatc tgtcgtagta cttgtcattt ttatccggat
      421 ttacggggtt ttgctatcct ttccgctcgt gctctgcttc ctgtttgcgg gatttctcct
      481 tcatttcccg tgcaaacgct tccgcattct tcaggtcttc tgtattcggc ctgcccttat
      541 tgaggccacc gaaatatttt aagaaactgt ttgtgttcca ccctgcacac ccgaattcac
      601 cgatcaccgt gtaccctttc gctttcagtt tttcccggat ctgcgagtgg ttattcttaa
      661 caaaatcgcc gccagcaaac acggccgggg ccccgtaggt tgaaaagagg aaggctttct
      721 tttccgcagc gtctggcagc cggtcggcaa ggtcaaggac ggatgggtcg aatgtcgcac
      781 tgtagatccc cgagccgaag ccaataaggt catattccgc gatctcttca ggcttaacct
      841 gctgcggcgt ttttacctca gcaccaagca cttttgcaca ggcgttcgcg atcttctctg
      901 tattgttatg atggtaggag aacacgatca caagagatcg taacaccttt ggatttccgg
      961 tatcaattcc attcttttcc atttttttca ccttccctaa tttattctcc tgtggcagac
     1021 ccggaagcga ttgaagacag gttgaatcct ttaactatca gccagaccgc cataaccatt
     1081 tcctgcaggg ccaatggaaa ttccagaact atctcgctcg ttgagagagg accaaagaaa
     1141 ccgaacaaaa ccagcaaccc tgcagccaga tagggtatgg ttgcaatgag accccagcct
     1201 gataaccacc ggggaatgag gtttgtctga tacaataaat agtaaaaaat cagggcaccc
     1261 aggcaaaaga cgattgtctg aacaggacca atcaaatcat gtactttgag tactacagcg
     1321 cccagggttt gaaaatacga agcatccgga gttcctgctt tgacgtattc ctgacttaat
     1381 ggtatgagga acagccagac aattgccaga ataatataca taacggtctc aagcgccccc
     1441 cggaaaacaa catacccgag agccagggct tcatgatatt ttttcaggat gggaaacatc
     1501 ataacgggaa ccatagcaag ggcaaggccc ataattaaca cacagagggc tcctgttatg
     1561 atctgcatct catgtgcaga aacgttaaca agataatccg gatctccgag aacaggtcct
     1621 gtaaaaacat aactgagcat gcctgcaatc gtcccgataa tatacaatac tcccacaatt
     1681 attgcggtct ttttgtttgt gtcatcagtt ttcatttcaa ttcctcctga ttttacgggg
     1741 gccggcacaa ctagtcccgg tcatgagctg gcctccggaa gaacctgttc gctctccttc
     1801 acgcctttca ccataagcca gccgcagaag gtaaattccg caataacggc gaccagaaaa
     1861 cctgggtacg taatcacttc aaaacccggg aaaaggaaaa cctgggcaac cccaatccag
     1921 tccccgatac atgcagccat cagcaggatg cccgatattt ttggcaggaa gccggattga
     1981 tacaccaggt atccaagggg aaacaaccag ccgccataaa atatcgcatc aatggtgtac
     2041 ccataccggt gcatatcgag aaagaacatc atcagggcgt ggagctgacc ggtactgaat
     2101 gcctgcgcaa gaccaggatt ggtcaggatc tgcagggcgg caacctggtt taccatattg
     2161 atgcactgta tggtgatact gattgcagca attacgacaa acagcagggc gatgtctttg
     2221 ttcaccggtt tcagtaagat gtacagggtc catgccagta acacaaaaca cgcacccatg
     2281 aacaggtcgc ttacgatacc gacacggaac agccattcat tggcgagaat gttaccggct
     2341 gttgttgcag cgtccccggg gacaataaga ccggcgcgga caaaaaattc cgcaaagatg
     2401 ccggtcacta tgataataaa ccaaaggatt cctgcgattc ttgccgtttt gtttcgtgat
     2461 atatcactgg tagggaatgt catggtattt tcctcaagtg actgatttca tgggcgattc
     2521 ctgttcaggc catttccagg cataccagac aatcacgaga agaagaacgc tttccagaat
     2581 gctcaaaaaa aagtaatacg cccattcttc cccgatacag aaaagtaaaa tcgtcgctgt
     2641 atacaataaa cccagtacga tgtttgccca gcgatttaca tcgggcctca aaaccaggga
     2701 cagaaaaacc atgagacagg gaatcgcaat gtagagggag gatcccacga ggaatccctg
     2761 attaatctgg aaacctgata tcttccccgc gattattccc tctataaggc cagtcctgaa
     2821 aaaactgaaa atatccccgt aggcaaataa aagcatcacg gctatccata atgctgaaat
     2881 cttcagcttc acatgtatct tcacatcttc cagaattccc ggtgtaagat ttgtcatttc
     2941 aattcatcct catttccttt gatgtactca ttttacctcg ttgatatccg tcctgaggga
     3001 caaggaagca attgcagacg gattgaatcc tttcacgatc agccataccg ccagaaccat
     3061 ctcctgcagg gctatgggga gatttaaaac aatatggatt gtcgacaggg gactgatgag
     3121 acccgacata accgacacgc ttgcaaccat gcacaacaca gctccggcaa gaccccagcc
     3181 tgacagccac cggggaatga gttctgtctg ataaaaaata gaataataca tcagggaacc
     3241 aagaccaaaa gcggtgggtc ctatcccaaa gcccgcccat tcacgccctg ccagtaataa
     3301 cgttcccagg atctgaaaaa aggaggcatc cggcattccg gcctgaacaa attcctggct
     3361 taatgtcagg agcagtacca ggccgaggat accaacgata tagaacactc cctcaataag
     3421 cctgaaacaa actgccccaa gagccaggcc ttcactatat ttcttcaata ccggatacag
     3481 cgagattgca atactggcag atgtgacagc agcaattaat atcaggagcg ctcctgccgt
     3541 tatctggttt ccattcgctg aaatttcaac aagataatct gaagaatgaa ccgattttaa
     3601 aaacagatta ctgagtatgc ttgcgaacgt cgcaatgatg agtaatgtac ctgcaatgat
     3661 tgcggtcttt ctgtttgtgt cataggttgt caattcaatt cctcctcctt tgctggatct
     3721 tgttttgatc cggtgatagt gttttcatct gcatctcctt cccaagctat ctttctgaaa
     3781 aaccggccgt gagtaattca tggcctgatt atgatcctgc aactcctgca gtttttgttg
     3841 tgacgaatct gcggagatgt gaatcaccag gtaaataaag tacaggaacc ctaccattcc
     3901 tgcggtctta ttgactgaat tcattttttg tctcctgtga aaagggaatg tgcaggttca
     3961 gtgtagagac agccaataac tattccgaga aggagaaacc ttgctccatt aaacattacg
     4021 ccgatgagga agtaatacaa ggcagcagaa gaattactca tcccaaaaaa actttgagga
     4081 acatcgatca ggattatggc aattatcagg gcgatgaagc ttattatccc ggatttcaga
     4141 atgggatttt tggatggaat cgcatcaaaa taacgaatca ggatataact gacacaatat
     4201 ccgacaatca aacctataag caaagagcca acccagac
//
LOCUS       DASRLV010000218         4097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1212294,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000218 DASRLV010000000
VERSION     DASRLV010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212294"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(721..1197)
                     /locus_tag="VFC43_08400"
     CDS             complement(721..1197)
                     /locus_tag="VFC43_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZK31229.1"
                     /translation="MDILKIPRMEKLEYDRLIEKGYICRIAFQGEKYPYIAPFLYAFD
                     GSFLYFLSTKYGKKIEYFRKSPYVSVEIEKYTKDLSSYMFVTLQGYLEEVHDSIEKQI
                     IREKFVDLIVERNLSCNILAALGHSPLDPPAAIAEEERSLVWKLVGVKDLVALKNL"
     gene            complement(1275..2642)
                     /locus_tag="VFC43_08405"
     CDS             complement(1275..2642)
                     /locus_tag="VFC43_08405"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZK31230.1"
                     /translation="MLFERVFSEGIAHNSYIIGSGGRAAVIDPRRDCDIYLEIARRNE
                     LVITHIFETHRNEDYVIGSLELQKRCGARIFHGDNLKFSYGNPVKEGDEFIIGSLLVR
                     VLETPGHTEESISLALRDPEVSDQPYMIFSGDTLFAGDIARTDFFGAERKPEMAAKIY
                     DSIMNKILPLGDGVILCPAHGAGSVCGGEIADHPFSTLGYEKKTNHHLTKGREHFISQ
                     RSTESPYYPPYFRQMEKYNQDGIGILHRKPDLVSLSIPEINQLRKSGCQIIDIRSPTS
                     FGAGHIPGSISLWREGLPSFMGWFLDYQKPIIIVDDFNLELDNVILHFIRLGYDNLAG
                     VLSGGFPAWTKAAQEIGTVPTCSVHQLKERLEKEKPFILDVRDIKNWRAVGHISGAHH
                     TYIGELPQHLDEIPTNQSIVVYCDAGYKGSLAASFLSIHKYRGVTNVLGGMTAWKRAG
                     FPVES"
     gene            complement(2722..4023)
                     /locus_tag="VFC43_08410"
     CDS             complement(2722..4023)
                     /locus_tag="VFC43_08410"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HZK31231.1"
                     /translation="MDDIYLIEIRLGRTKWRIRKTIFRFGGLFSLESSIEQHPHVTLF
                     GPLTLNEDTSPKQLIDTIGKITDGYGPIPFTINGWELREGIHGSVIAFTVIPSEPLKN
                     LTSSLAESLASLVQSQNIWDTRPENKWFHITIANRLDLHRALAVFSTLTGQKKAVQDQ
                     GVLQRLKHIVQCMFIWTRGHAVPPVTIDEHGLRLTVMRGDMIFAEFDLLENRWISGDY
                     SHKGKSWQNSLSLFRQKSGFERSDPLPSNPEDIFLIGDLHLGHANIINYCSRPFLTSD
                     VREMDHVLIKNWNYTISPANRVYHLGDLRYGLDAPPAPEYRKKLKGQITFIGGNHDED
                     GSDSVPSVNLEYQGFCFQLVHDPADVKKEFDGWIIHGHHHNNDLRNYPFIDFEHRRIN
                     VSAEVLGYIPVNLKHICTIINERLSNGNKTAILLNYPYVGE"
BASE COUNT         1120 a          942 c          942 g         1093 t
ORIGIN      
        1 ttgttacagg agtcaaaccc gaaaaaatag cacaaaaaaa gttgatacaa aatattttta
       61 aaataattgc tcagtagaaa cagacatgaa cgggaattta cacccgaacc atgaaaatcg
      121 tacaatatat gatgcaccct gcctcagggc ctctccgagg cacggtggga cggatcggct
      181 gtttaaaatt tttgttgctg atccgccgcg ggggcgcccc gtcaggggcg gcggcgttca
      241 tcgtacctga agaatagatc tgataatccc cttcatcata cctgaatgaa tcgattttca
      301 tgggaaatcc tgttttaaag gagcaatctt accgaagacc aggcaggata ttattgtcat
      361 cgggggttat ttttaccgtc aaaaaactcc tgtgagaatt ctggtacacc ggagcctggt
      421 acgagcccgg ttgtacctca tttcccccac cccccgaatt tcagaggtca taattcagaa
      481 aaatgcggaa ccgggccgga attattcaac gatttccgac gggaacggat gaggtcgaat
      541 atagcaatgt tacaaagacc aaaaagcagg aggggatgtg gtttgggacg cggagggcac
      601 cggttagggg atattccagc atccttcccg gaaggcgtca ggcataaaga tatgagaaca
      661 agaagggtgg attctacaga gcaaaaataa ttataaatga catccatccg gatggataca
      721 tcagagattt ttcagtgcaa cgaggtcctt gacaccgaca agcttccaga caagcgaacg
      781 ctcctcttct gcaatcgcag ccggtggatc caggggggaa tgaccaagcg ctgcaaggat
      841 attgcaggat agatttctct ctacaatcag atcaacgaat ttttcccgta tgatctgttt
      901 ttcgatagaa tcgtgaactt cctctaagta accctggagg gtcacgaaca tatacgagga
      961 aaggtctttt gtatattttt caatttcaac ggaaacatac gggctttttc ggaaatactc
     1021 gatctttttt ccgtattttg tagagaggaa atagaggaat gaaccgtcga aggcatagag
     1081 aaacggtgcg atatacggat atttctcacc ctgaaatgcg attcggcaga tatatccctt
     1141 ctcaataagc cgatcgtatt ccagtttttc catacgcgga attttcaaaa tgtccataga
     1201 ctcacaccaa tggagttccc aacattacat gatatacttt attaattttt tttgaaacaa
     1261 taaaaacaat aaaattatga ttcaaccggg aagcctgccc gtttccaggc cgtcatcccc
     1321 cccagaacat tggttacccc ccgatatttg tgtattgaga gaaagctcgc agcaagactc
     1381 cctttatacc cggcatcgca atagaccaca atggattgat ttgttggaat ttcatccaga
     1441 tgctggggaa gctccccgat gtacgtatga tgagcaccgg atatatgtcc gacagcgcgc
     1501 cagtttttaa tgtcccggac atcaagaatg aacggtttct ctttttcgag gcgttccttt
     1561 aattgatgga ccgaacatgt tggcacagtg ccaatttcct gtgctgcttt ggtccaagcc
     1621 ggaaatcctc cggaaagcac tccggcaagg ttatcataac ccagccggat aaaatgcaaa
     1681 ataacattat caagctccag attgaagtca tcgacaataa tgatcggttt ctggtaatcg
     1741 aggaaccacc ccatgaagga gggaagacct tcgcgccaca gggagatgct gccagggata
     1801 tgtccggcac cgaaactggt aggtgagcgt atatcgataa tctggcagcc gctctttctg
     1861 agctggttaa tctcaggaat agagagggat acaaggtccg gcttacggtg caggatccct
     1921 atgccatctt ggttatattt ttccatctga cggaaatagg gggggtaata aggagattcg
     1981 gtactccttt gtgagataaa atgctccctc ccttttgtga gatgatgatt tgtctttttt
     2041 tcatacccta gtgtgctgaa cgggtgatca gcaatttcac caccgcagac agacccggcc
     2101 ccgtgagcag ggcagagtat cactccgtcg cccagaggaa gaattttgtt cataatgctg
     2161 tcatagattt ttgcggccat ttccggtttc ctttccgctc cgaaaaaatc ggtccgggca
     2221 atatccccgg caaaaagtgt atcaccggaa aatatcatat acggttgatc gcttacctca
     2281 gggtcacgca gcgctagcga tatactctcc tcagtatgtc ccggggtctc aaggactctt
     2341 actaaaagcg atccgatgat gaactcgtca ccctctttta ccgggttccc ataggaaaac
     2401 ttcaggttat caccatggaa aattctggct ccgcatcgtt tctggagttc cagcgaacct
     2461 ataacataat cctcgttgcg gtgggtttcg aagatatggg taatcaccag ttcattccgc
     2521 cgggcaattt caaggtatat atcgcagtca cggcgtggat cgattaccgc agccctgcct
     2581 ccggatccaa taatatatga attatgggca attccttcag aaaaaacccg ttcaaacaac
     2641 atagcctgat tccctctatc gtggaattac acactgtggc tgtataaaag ttatgctgag
     2701 gaagtacctg cgacctggtg cctattcccc gacataggga taattcagaa ggatagccgt
     2761 tttatttccg tttgaaaggc gttcgtttat gatagtgcag atatgcttca gatttaccgg
     2821 aatgtaccct agtacttcag cactcacgtt gatccgacgg tgctcaaagt caatgaatgg
     2881 ataattacgc agatcattgt tgtgatgatg accatggatg atccagccat cgaactcttt
     2941 ttttacatcc gcaggatcat gaaccagctg aaaacaaaag ccctggtatt ccaggttaac
     3001 cgaaggaaca gaatctgatc catcttcatc atggtttccg ccgataaagg tgatctggcc
     3061 tttgagtttt ttcctgtact cgggagccgg cggcgcatcc aggccatacc gtaaatctcc
     3121 cagatgatac acacggttgg ccggtgaaat ggtatagttc cagtttttta tcaatacatg
     3181 gtccatttcc cggacatcag aggtaagaaa cgggcgcgaa cagtagttga tgatattcgc
     3241 atgaccgaga tggagatctc cgataagaaa tatatcttca ggatttgatg gcaggggatc
     3301 tgatctttca aacccggatt tctgcctgaa aagggagagg gaattttgcc aggatttacc
     3361 tttatggcta taatccccgc tgatccaccg gttctccaga agatcaaact cagcgaatat
     3421 catgtcccct ctcataacgg tgagtcggag tccatgttcg tcaatggtca caggagggac
     3481 tgcatggccc cttgtccaga taaacatgca ctgcacaata tgcttcaatc gttgtaagac
     3541 accctgatcc tgcactgcct ttttctgacc ggtgagtgta gaaaaaaccg ccagtgctct
     3601 atgtaagtcc agacgatttg ctatggttat atgaaaccat ttattctcag gccgggtatc
     3661 ccagatattc tgactctgca caagggaggc cagactctcc gctaacgatg aggtgaggtt
     3721 ttttaagggc tctgagggga tgaccgtgaa ggcaattaca cttccgtgta ttccctcccg
     3781 aagttcccat ccattaatag tgaatggtat aggaccgtat ccgtcagtta tctttccaat
     3841 agtgtcaata agctgcttcg ggctggtatc ttcattcagg gtcagaggcc caaacagggt
     3901 cacatgcgga tgctgctcaa tgctactttc cagggaaaat aatccgccga aacgaaaaat
     3961 tgttttcctg atccgccatt ttgtcctgcc gagacggatc tcaataaggt aaatatcgtc
     4021 catgaaaggt actctgcacg tatgttctat atgcggtctt tgtggttaaa tctttgattt
     4081 gattttttcg taactat
//
LOCUS       DASRLV010000219         4008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_649173,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000219 DASRLV010000000
VERSION     DASRLV010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649173"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..1339
                     /gene="serS"
                     /locus_tag="VFC43_08415"
     CDS             62..1339
                     /gene="serS"
                     /locus_tag="VFC43_08415"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HZK31232.1"
                     /translation="MLDIRFVRSSPDIVRADLEKRNDTEKLAWVDDLLKKDVRSRELK
                     IETDTLRQRRNTIAREINALRKSGQEASLLMAEAAALPQKIREYDEERDEIAHAIRHY
                     LMRLPNILDPGVPVGKDDTENVEIRRVGTVRTFDFELKNHGQLAADKGWADFERATKI
                     SGAGFYFLKGSLVLLDMALQRYAIDLLVRKGFTPIIPPYMINRPSYEGVTDLEDFEKV
                     MYKIEGDDSYLIATSEHPLGAMHKDEIFEEKDLPLRLAGISPCFRREIGAHGLDTKGL
                     FRVHQFTKVEQFVFCKPEDSWRIHDELLSNAEEVFQNLEIPYRVVNICTGDIGTVAAK
                     KYDIEAWMPREKEYREVVSCSNCTSYQAVRLNIRVRDKSEFESKQNVHTLNSTAIATS
                     RVMRAILENCQCKDGSVEIPRVLRPYINDKEYL"
     gene            1596..3137
                     /locus_tag="VFC43_08420"
     CDS             1596..3137
                     /locus_tag="VFC43_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31233.1"
                     /translation="MEAGENAIRDHFLILAILTGSGEKQQTVLTFLRSAGGERKRECN
                     EWARKLKSLIISPSRETDVVPGLKPERPVLTKTDVPESAQSGPGGTRPAKKKIEIVRP
                     LGTIIEKSPVAPQPVEISSLPSGTFCSRCGNRVPHKSPFCNYCGTPIINVPAPAVQQP
                     AVSREQVPVPPLTSQPVIPRVISGPEPVAPSPVVPKVQVPPLVIPQPDISRGPSEPEP
                     AAPSIEVPKVPIPVPPVTGSAPEPAGSPINQIIHSIEPLIEDSVPRTHPAPVFKKPVS
                     EHTPKSEHTPKPVITEVLAESPPQVVWPVLPTSESPGEPARVDIPAEPESPLQPPAPA
                     PGGKKPNYLAIGIIIIAILPIIAGIVIVANMMAAPSGISGATPTITTVVTTISTPKPQ
                     LSLTPEETTILAPGQTQLMIPPSGVWVRASYSGTYIGLIGTPGNQIEVSGTGDKFYPI
                     STSDGIVAAVLQKKDNSGDKIILEIYKNGVMVKRDTTTTPSGIVEIQLDLRSLPATGN
                     STSSK"
     gene            complement(3222..3683)
                     /locus_tag="VFC43_08425"
     CDS             complement(3222..3683)
                     /locus_tag="VFC43_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31234.1"
                     /translation="MQWIHALEIILVLGIITAGCIGSSSPSKPVISTTPLVVNDTVPI
                     TPDPTRIIDNLQDPIIGIWWENYSFGYDDRFRFNADGTFVESFSLGDKKRTLVTHGTW
                     RANGSNSYVLMDAKNEAYITFIHDPEQNVIYPQKNSISVLTPYRWDVITVD"
     gene            3818..>4008
                     /locus_tag="VFC43_08430"
     CDS             3818..>4008
                     /locus_tag="VFC43_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31235.1"
                     /translation="MDSISVIIANRVICQHQKDYGLGIIIFIAFGEEPVKGNTVAQRQ
                     EINNDDTHHRDPVLHYEVT"
BASE COUNT         1158 a          962 c          897 g          991 t
ORIGIN      
        1 aaattatgat atacatccgt gctataatac atagaaacgc gtacacgaga aggtatactc
       61 aatgcttgac atcaggttcg tacgatcaag cccggatatc gtcagggctg atcttgaaaa
      121 acgcaatgat acagagaagc tggcgtgggt agatgacctg cttaaaaaag atgtccgctc
      181 ccgcgaactt aaaattgaaa cggataccct gcgtcagcgc cggaacacga tcgcccgcga
      241 aatcaacgcc ttgcgaaaat ccggtcagga agccagcctt ctcatggcag aagctgccgc
      301 actccctcag aaaatcaggg aatatgatga ggaacgggac gagattgcac atgcgatccg
      361 tcattatctc atgcgcctgc ccaatatcct ggacccgggc gttcccgttg gaaaagacga
      421 cacggagaat gttgaaatca ggcgtgtggg cacagtcagg acatttgatt tcgaactgaa
      481 gaaccatgga caactcgctg ctgacaaggg atgggcggat ttcgaacgtg cgacaaaaat
      541 ctccggagcg ggtttttatt tcctgaaagg cagccttgtc ctcctcgata tggccctcca
      601 gcggtatgca attgatctgc tggtgcggaa aggttttacc cccataatcc ccccctacat
      661 gattaatcgt ccttcctatg agggcgtgac agacctggaa gatttcgaaa aagtcatgta
      721 taaaattgag ggtgatgatt catacctgat tgcaacaagc gaacaccccc ttggtgccat
      781 gcataaagat gagatctttg aagaaaaaga tctcccgctc cgccttgccg gtatttctcc
      841 gtgcttccgg cgtgagatcg gagcgcacgg cctggataca aaagggctgt ttcgtgttca
      901 ccagtttacc aaagtagagc agtttgtatt ctgcaaacct gaagattcat ggcggattca
      961 cgacgagctg ctgtcaaatg ccgaagaagt attccagaat cttgagatac cataccgggt
     1021 ggtaaatatc tgtactggtg atatcggaac cgttgctgcc aaaaaatatg atattgaagc
     1081 ctggatgccc cgggagaaag agtacaggga agttgtatcc tgttcaaatt gcacctccta
     1141 tcaggcagtc cggctgaata tcagggtcag ggataagagt gaatttgagt caaaacagaa
     1201 tgttcatacc ctcaattcaa ctgcaattgc aacctcaagg gtaatgcgtg caattctgga
     1261 gaactgccaa tgcaaggatg gttccgtgga aatccccaga gttctccggc catatataaa
     1321 tgacaaggaa tacctttgat tcattatgtc cggaaataaa aggggcctgc gaacaaatac
     1381 ttatttgctg ttgatagcgc aactataggt aagggtgtgg tactataggc tatcctgaac
     1441 tggaccgcga cgagtccata atcctggaaa ccagaaatgt aaaatataag tccatctcgt
     1501 ttgatgcgat tcttaccaat aaacgtatta ttcttaccga tagtaaaaag agtggaattc
     1561 cctcgcagga tatccctctc tcaactatca ggaatatgga agcgggtgaa aatgctatcc
     1621 gcgaccattt tttaattctt gctatattga cgggttcggg agaaaagcaa cagaccgttc
     1681 ttacattttt acggagtgcc ggtggggaaa gaaaacggga atgcaatgaa tgggcccgga
     1741 aattaaaaag tcttataata tccccctcca gggaaacaga cgttgttcct ggcctgaaac
     1801 ctgaacgtcc cgttttgact aaaactgacg ttccggaaag cgcacaatct ggtccgggag
     1861 gtacacgtcc ggcaaaaaag aagatcgaaa tcgtccgtcc tctgggaacg attattgaaa
     1921 aaagtccggt ggctcctcaa cccgttgaga tatcctctct tccttccggt actttctgtt
     1981 cacgatgcgg gaatcgtgtc ccccataaat ccccattctg caattactgt ggcaccccaa
     2041 taatcaatgt tccagcaccg gcggttcagc aaccggccgt ttcccgggaa caggtgccag
     2101 tacccccgtt aacatcacag ccggttattc cgagggttat atcagggccc gaaccggtag
     2161 ccccatctcc cgtggttcct aaggttcagg taccaccact ggtgattcca caacccgaca
     2221 tttcccgggg tccatcagag cctgaaccgg cagcaccttc cattgaggtt ccaaaagtac
     2281 cgatacccgt acctccagtg acgggttctg caccggaacc tgcgggtagt cccattaacc
     2341 agatcatcca ttccattgag ccgctgatag aggactcggt tccacggaca caccccgcgc
     2401 ctgtgtttaa aaaacctgtt tctgaacata cacccaaatc tgaacataca cccaaacccg
     2461 ttataaccga agttttagca gaaagccccc ctcaggtagt ctggccggta ttaccaactt
     2521 ctgaatctcc tggggagccg gccagggttg atatccctgc agaacccgaa tctcctctgc
     2581 aacccccggc cccggcgcca ggtggaaaaa aaccaaatta tctggcaatt ggaatcatca
     2641 ttatcgctat cctgcctatt atagccggga tagtaatagt cgcaaatatg atggcagcac
     2701 cttccggaat ttctggagca acaccaacga taactaccgt tgtgacaaca atttcaacgc
     2761 caaaaccgca actttccctt acaccggaag aaacaacgat tttagctccc gggcagacac
     2821 agctgatgat tcctccgtcc ggagtctggg tgagggcatc gtattccggg acgtatatag
     2881 ggttgatcgg aacaccgggg aaccagattg aagtgagcgg cactggagat aaattttatc
     2941 ccatatccac aagcgatgga attgttgcgg cagttctcca gaaaaaggac aattccggag
     3001 ataaaatcat tcttgagatt tataaaaacg gggtcatggt caaacgagac acgaccacta
     3061 caccatcagg tatcgtcgaa atccagcttg atttacgatc cctgccggca accggaaatt
     3121 caacatcatc taaataataa tcactccgtg gatctcagca aaaaagagac agaaggaatc
     3181 gtcctacgct attgaagaaa aatctttaat ccgggaaatt attaatcgac cgtgatcaca
     3241 tcccatctat acggtgtgag cactgaaatg gagtttttct gaggataaat cacattttgt
     3301 tcagggtcat ggataaatgt gatgtacgct tcatttttcg catccatcaa tacatatgaa
     3361 ttactgccat tggccctcca cgtcccgtga gttaccaaag tccttttttt atcacctaaa
     3421 gagaaacttt caacaaacgt accatcagca ttaaaccgga acctgtcatc ataaccaaat
     3481 gaataatttt cccaccagat accgatgatg ggatcctgca ggttgtcaat tatacgtgtc
     3541 gggtctggtg tgatcggaac cgtgtcattc acaaccagtg gtgtggtcga tatgacaggt
     3601 tttgacggag aagacgatcc aatacatcct gctgttatga ttcccagaac cagtattatt
     3661 tccagtgcat ggatccactg catgcttatc ttttcattta gctgatacta taaacctttc
     3721 aaaaaattag tttttagtgt ttttttaccg tctggctgga cgaatcatac agaggttgtt
     3781 gaaggatttt tcttgaatgg aatagtaagt tacttaattg gacagtatct ctgtcattat
     3841 cgcaaatcgc gttatatgtc agcaccaaaa agactacggt ctaggcataa taatttttat
     3901 cgcatttggt gaagaaccag ttaaaggaaa tactgttgct caaagacaag aaataaacaa
     3961 tgatgatacc catcacagag accccgtttt acattatgaa gtcacaaa
//
LOCUS       DASRLV010000220         6436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1052857,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000220 DASRLV010000000
VERSION     DASRLV010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 6436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6436
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052857"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VFC43_08435"
     CDS             complement(<1..312)
                     /locus_tag="VFC43_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZK31236.1"
                     /translation="MEDNHTIWIEKYRPSKLADIVGQDEIVERLSSYVKTGTLPHLLF
                     TGSAGVGKTTAAVTLAHEFFKDSWQMNFRELNASDERGIDVVRNQIKQFARTTPLGNA
                     TF"
     gene            454..1485
                     /locus_tag="VFC43_08440"
     CDS             454..1485
                     /locus_tag="VFC43_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3821 domain-containing protein"
                     /protein_id="HZK31237.1"
                     /translation="MHGKFIVLAFIIIFMYVAPVAGNTLKIAAGAPVYIGESDLDIAK
                     ALNGCHTISWWQNGTESSAPPQKNITLYEINSVSDIINHYTISPKIFTGYTGTWYCTD
                     KKPPKAVFEVLEPTLDIKVWDLDQNQDISGKSVPVSTKITYRVDTNLYQALKPLNRPD
                     ITPTDSFFTVTMTDSYNQNTSNIFTGSAGKPKTQILTFDKQPYITVSPFYWKNGKDWD
                     RSARNAKGEPLYPSSMYTFTISQNLNHIQETYRANGISDFTGKITDTATITFVKDNAS
                     LVTTVTTPPVATTMPLTTITASGTEGVSPLPTSTPVTGKTTYSPLAEWITLLGVGMAG
                     LLMITRNRQ"
     gene            1546..2100
                     /locus_tag="VFC43_08445"
     CDS             1546..2100
                     /locus_tag="VFC43_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZK31238.1"
                     /translation="MKLHKILWTMLVVSVVILPVMATDSPDQLNNAGALYSQSVDLAQ
                     EGKYQEALQASDRALALNVTSMVSVIQANRAGILVMLGRFDEAIEAADISLAREGNLT
                     TAHSIAWYNKGNALRSLGRTDEARTAYARAAELDPTLIPPDLATVTPVVSLTPAKSPV
                     QWSTVLLALSLVSCIWVCLYQKRK"
     gene            2353..3123
                     /locus_tag="VFC43_08450"
     CDS             2353..3123
                     /locus_tag="VFC43_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31239.1"
                     /translation="MEGFIRIFAGEYDSSTLSLQGVDSQSPPWVVTPGGGYCRFMFLS
                     GTLTEVSETGDMVNSRVADPTGVFNLVIGGRNSSLAEAFRNLTIPSFVTVTGRSQIYQ
                     KDGRFDVSVRPEYVQNVNRQVRDQWVLSAAQSTLGRLFLMNQALQGACTDDRVLAAFH
                     HYSITNSRLRELAFMVEGAVNTVQTVECELPVQPDVRTVVMDLIRAGSGPKGISVDQI
                     INQATAYGISQETVLSIIESLIIDDECYQPLKGFVKPL"
     gene            3126..3875
                     /locus_tag="VFC43_08455"
     CDS             3126..3875
                     /locus_tag="VFC43_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZK31240.1"
                     /translation="MEFIGDGPALVIENELRLLIVADLHFGIEADLAAHGLHFKSRSA
                     VRLDHLLKTIDKADPDQLIILGDLKHSIPSLTRQEYFELPEILDALRNRIPLKVFPGN
                     HDIGIERYLLKTEIQPKDGAVLDGVGFLHGHMYPSPQLSGHLMVVGHHHPLLSLKDEV
                     GCAIQAPAYLRAGLDAKGLGLDPDQKDGPPPRVLFMPAFNEIAGYDILQIIKNPFSPV
                     SRFMKKEEAEIILADGTYIGPVRFLNQDETD"
     gene            3862..>6436
                     /locus_tag="VFC43_08460"
     CDS             3862..>6436
                     /locus_tag="VFC43_08460"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZK31241.1"
                     /translation="MKPIEVLHPKVQACIKKRGFSVLSDAQKQAIPLILKGDHVLLIA
                     PTGTGKTESAMFPVFNELLSMPSGGGIKALYITPLRSLNRDILARMQWWCGELGLSVG
                     VRHGDTPLNERRKQALSPPDLLITTPETLQALFMGKRLRKHLAQVRFVIIDEIHDLAG
                     SKRGAQLAVALERLYVHAGEFQRIGLSATVGNPEDIARFLCGKRPFSIVQVPVAKQLD
                     ITVRFVGDNFEEQITILSKALNREGSTLVFVNTRVTAEALGHALYERGDVEVHHGSLS
                     KEVRIDAEERFKRGEIRTLICTSSMELGIDIGRVDHVIQFGSPREVARLVQRVGRAGH
                     QLNTISRGTILATGFDDLLESLVIAKKARANEIERVSPHTNAADVLANQIAAIAVEYG
                     EIEQAKIREILERTSVFTQSGSLLDDVCRQLEEHRLIRLDGSRIITTARARRYLTANL
                     SMIHDERKVPIFDMVSRRTVGTLDESFVVGWVHTGAIFITKGQLWRIIEIADGKLIVE
                     PAKKSQGELPSWEGEQIPVPFSVAREVGGMRRKRDIAAYAAEHDGITFAEKFLHEMDI
                     NRSLIPTDHLITLENTDEGVVCNVCAGHKANEALGRVLSILISARHGTTVGLELDAYR
                     MLLRLPSSLRAADVRDLLLSLDPAHMTGIVRLALKRTALFKWKLVQIAKKFGAIDPDA
                     DYEKISIQRLLEYFDGTVVQQEAYRELLLEYMDINAAIAIVDLIVKGEIQVALGPHSL
                     IGAGGLLSSRDQIPPPTADKAVIETLKRRLEQDDVLLVCMNCRDWKSRTVVSRVPDHP
                     QCPKCGARLIAALKPYEDELYTLIRKKSKTQEERMIDKRLLKNANIVLSSGRKAVLAL
                     SA"
BASE COUNT         1635 a         1562 c         1572 g         1667 t
ORIGIN      
        1 aaacgttgcg tttcccaggg gagtcgtacg tgcgaattgc ttgatctggt tacggacaac
       61 gtcaattccc cgttcatccg aggcgttcag ttcacggaaa ttcatctgcc atgaatcctt
      121 aaaaaattca tgtgccagtg tcactgcagc agtagtcttg ccgacgccag cactaccggt
      181 gaaaaggagg tgaggaagag ttccggtctt cacgtaggat gataaccgct caacaatttc
      241 atcctgcccg actatatctg cgagttttga cgggcggtat ttttcaatcc agatcgtatg
      301 attatcttcc atgagcactc ttcctgctta ctgtttaatt cattctcgga tagtatgggt
      361 agtaattccg tgggaacgtt gaatgtaaaa aatcacacat tcttttttat ccatgtcata
      421 atgtttatca taagtgtttt gaaattactt tccatgcatg gaaaatttat cgtacttgcg
      481 tttatcatca tttttatgta tgtcgcacct gtggccggca ataccctgaa aatagctgcc
      541 ggggccccgg tctatatcgg cgaaagtgac cttgatattg ccaaagcgct gaatgggtgt
      601 catacaattt catggtggca aaacggtact gaatccagcg cgccaccgca aaaaaacatt
      661 accttatacg aaataaattc ggtttcggat ataatcaacc attacaccat cagtccaaag
      721 atttttaccg ggtatacggg cacatggtat tgtacggata aaaagccacc aaaagctgtt
      781 tttgaagttc ttgagccgac actggatata aaagtctggg atctggatca aaaccaggat
      841 atcagcggga aatcagttcc ggtttctacg aaaataacgt accgggttga tacgaacctt
      901 taccaggcgc tcaagccact aaatcgtcct gatattaccc ccacggattc attctttacg
      961 gttaccatga cagattccta taaccagaat accagcaaca tttttaccgg aagtgccgga
     1021 aagccgaaaa ctcagatcct gacctttgat aaacaacctt acatcacggt atcccctttc
     1081 tactggaaaa acgggaaaga ctgggatcgc agcgcgcgga atgcgaaggg tgagccactc
     1141 tatccttcct ccatgtatac gttcaccata agccagaatc ttaatcatat acaggagacc
     1201 taccgggcga atggcatttc cgattttacc ggaaagatca ccgatacagc aacgattacg
     1261 ttcgttaaag acaatgcttc tcttgtcaca acggttacga cgccaccagt agcgacaacc
     1321 atgcccctga caactatcac tgcatcagga actgaaggtg tatcaccatt acctacctca
     1381 acgccggtca ccggtaaaac aacctattcc cctctggcag aatggattac cctgctcggt
     1441 gttggaatgg cgggattatt gatgataacc agaaatcgcc agtgattgcc caatccattt
     1501 ttttttaagg atatactttc aatgaataca agaaagagca attctatgaa attacataaa
     1561 attttatgga caatgctggt tgtttctgtc gttatcctgc ctgttatggc aacagattca
     1621 ccagaccagc tgaacaatgc aggtgcactc tattcccaga gtgtcgatct tgcacaagaa
     1681 gggaaatatc aggaagcttt acaggcatct gaccgggctc tcgccttgaa tgtgacctct
     1741 atggtctcgg tgatacaggc aaacagggcc gggattcttg tgatgctcgg gcgctttgat
     1801 gaagctattg aagcagcaga tatttcactg gcacgtgagg gtaatttaac aaccgctcac
     1861 tcgattgcct ggtataacaa aggcaatgcg cttcgctccc ttggcaggac tgatgaggcc
     1921 aggactgcgt acgcacgggc tgctgagctc gatccgaccc ttatcccccc ggatcttgca
     1981 actgtcacac ctgttgtgtc actgactccc gcaaaatccc cggtgcaatg gtcaactgtg
     2041 ctcctcgccc tctcgctggt ttcctgtatc tgggtctgcc tctatcagaa gaggaaataa
     2101 cccccggtcg ttcttttttc atcccgtccg ggattaatca aaatagattt tctcaccttt
     2161 cagctgcctc catgactgct ccattgagcg tttattcatt tcctctttta aaaaaaagta
     2221 tgatagccaa cggtatttgt ccttttaggg tgtgcgcctc gtattcagac aatcttttca
     2281 tcatagcgat tagtccggta tggtaatgac tatatcttga atacggttac ctgataattc
     2341 ttttatagca taatggaagg gttcatcaga atatttgccg gagagtacga cagctcaaca
     2401 ctatcattgc agggagttga ttcccaaagc ccaccatggg tagtgacacc gggtggtgga
     2461 tattgtcgtt ttatgttcct ttcaggtacc cttactgagg taagcgaaac aggagatatg
     2521 gtgaattccc gcgttgctga tccgacagga gtatttaatc tggtgatcgg tggcaggaat
     2581 tcttcccttg ccgaggcatt ccggaatctt accatcccct cctttgttac cgtcacgggg
     2641 agatcgcaga tataccagaa agacggcagg tttgacgtat ctgtccgtcc tgagtatgtc
     2701 cagaatgtta atcgtcaggt acgggaccag tgggtactat cagcagcaca atcgaccctg
     2761 ggacgtctct tcctgatgaa ccaggctttg cagggtgcat gtactgatga tcgtgttctt
     2821 gctgcctttc accactattc tataaccaat tcccggctca gggaacttgc atttatggtt
     2881 gaaggggcag tcaatactgt ccagaccgta gaatgcgaac tgcctgtgca gccggatgtc
     2941 cgtaccgtgg taatggatct catacgtgca ggcagcgggc caaaagggat ctctgtcgat
     3001 cagataatta accaggcaac agcatacgga atatcccagg agactgtcct ttccataatc
     3061 gagtcactta ttatcgatga tgaatgctac cagcctctga agggatttgt aaaaccatta
     3121 tgaacatgga attcatcggt gatggtccgg cacttgtcat tgaaaacgag ctccggttat
     3181 tgattgttgc agaccttcac tttggcatag aagctgacct tgctgctcac ggtctgcatt
     3241 ttaagagtcg cagtgctgtt cgcctcgacc atttgctgaa gactatcgat aaggcagatc
     3301 ccgatcagct cataattctt ggggacctca aacatagtat tccatccctg acccggcagg
     3361 aatattttga gttaccggaa atccttgatg cccttcgtaa tcgcatcccg cttaaggtat
     3421 tcccgggtaa tcacgacatc gggatcgaac gatatctgct caaaaccgag atccagccaa
     3481 aagatggagc tgtcctggat ggcgtcggtt tccttcacgg gcatatgtat ccctccccgc
     3541 aactctccgg gcatcttatg gtagtcgggc accaccatcc cctgctttca ttaaaagatg
     3601 aggttggctg tgccatccag gcccctgcct acctgcgggc cgggcttgat gcaaagggtc
     3661 tgggtctgga tcctgatcaa aaggatgggc cgccaccccg tgtgctgttc atgcctgcct
     3721 ttaatgagat tgccggctat gatatccttc agattatcaa aaatcctttc tcgccggttt
     3781 caagattcat gaaaaaagaa gaagctgaga tcattcttgc cgacggaaca tacatcgggc
     3841 cggtacgttt tctgaaccaa gatgaaaccg attgaagtcc tgcatcccaa ggttcaggcc
     3901 tgcataaaaa agcgcggttt ttccgtgctg tccgatgcac agaaacaagc aatccccctg
     3961 atcctgaaag gcgaccacgt cctccttata gccccgactg gtacaggaaa aaccgagagc
     4021 gcgatgtttc cggtttttaa tgaactctta tccatgcctt ccgggggagg aattaaagca
     4081 ctttacatca cgcccctgcg ctcactcaac cgtgatattc tggcacggat gcaatggtgg
     4141 tgcggtgagc ttgggttatc ggttggggtg cggcatgggg atacgcctct gaatgaacga
     4201 cgcaaacagg cattgtcccc tcccgatctt ctcattacta ctccggagac tttacaggcg
     4261 ctctttatgg gtaaacgcct tcggaagcat ctggctcagg tcaggtttgt gattatcgat
     4321 gaaatccatg acctcgccgg gagtaaacga ggtgcccagc tcgcggtcgc acttgaacgg
     4381 ctttatgtgc atgccggaga attccagcgt atcggcttgt cggcaacggt cggaaatccc
     4441 gaagatattg cacgttttct ttgtggtaag cgcccttttt ccatagtcca ggtacccgtt
     4501 gcaaaacaac tggatattac cgtcaggttc gtgggagata attttgaaga acagatcacc
     4561 atcctgtcaa aagcgttgaa ccgggaggga tcaaccctcg tttttgtgaa tacgcgggtt
     4621 actgcagaag cgctgggtca tgcactgtat gaacggggtg atgtggaagt acaccatggc
     4681 tctctgtcta aagaagtgag aattgatgcc gaggaacggt ttaagcgcgg ggaaatccgg
     4741 acgcttatct gcacatcatc catggagctg gggatagata ttggcagagt cgaccatgtg
     4801 atccagtttg ggtccccccg tgaagttgcg cgacttgtcc agagggttgg gcgtgccggt
     4861 catcagctga ataccatctc ccgtgggacg atccttgcaa cagggtttga tgacctcctt
     4921 gaatccctgg ttattgcaaa gaaggcaagg gcaaatgaga tcgagcgagt atctccacac
     4981 acgaatgctg cggatgttct tgcgaaccag atcgcggcga ttgcagtgga gtacggcgag
     5041 atagagcagg caaaaattcg tgagatactg gaacgaactt cggtgtttac tcaatccggg
     5101 agtctccttg atgatgtgtg ccggcaactg gaagaacacc gcctgatacg actcgatgga
     5161 agccggatca tcacaactgc ccgcgccaga cgatatctta ccgcaaatct gtcgatgata
     5221 cacgatgaaa ggaaggtgcc tatttttgat atggtttccc gaagaaccgt ggggaccctc
     5281 gatgaatctt ttgtcgtcgg ctgggtgcat acaggtgcaa ttttcattac caagggccag
     5341 ctctggcgta tcattgaaat cgcggacggg aaacttatcg tggaaccggc caagaaaagc
     5401 cagggggaac ttccttcatg ggaaggtgaa cagatccctg taccgttctc agtagcccgt
     5461 gaagttggag gaatgcgccg gaaacgagac atcgcagcat atgcagcaga gcacgacgga
     5521 attacttttg cagaaaagtt ccttcatgaa atggatataa accgctccct gatcccgaca
     5581 gatcacttaa taacactgga aaataccgat gagggggtgg tctgtaatgt ctgtgccggg
     5641 cacaaggcga atgaagctct gggacgagta ctctccattc tcatatccgc acggcatggg
     5701 actactgttg gtcttgaact tgatgcatat cgcatgctcc tgcgccttcc ctcctcatta
     5761 cgggctgcag atgtgcggga tcttttactc tctttagacc cggcacatat gacgggaata
     5821 gtccggcttg cactgaaacg cacagcactt tttaaatgga agctggtgca gattgcaaag
     5881 aaattcggtg caatcgatcc ggatgctgat tatgaaaaga tcagcatcca gcgccttttg
     5941 gaatattttg acgggacggt tgttcagcag gaagcgtacc gtgaactgct tttggaatat
     6001 atggatatta atgctgcaat cgctatcgtc gatctgattg ttaagggaga aattcaggtt
     6061 gcgcttggcc ctcattcact gatcggtgcc gggggtctgc tctcatcacg ggatcagatc
     6121 ccgcccccta cggcggataa agcggtgatt gaaacgttga aacgaaggct ggagcaggac
     6181 gatgtactgc tcgtgtgcat gaactgccgt gactggaaga gcaggactgt tgtttcccgc
     6241 gtgcccgatc acccgcaatg tccaaaatgc ggtgcacggc ttattgctgc gctaaaaccc
     6301 tatgaagatg agttgtatac ccttatccgg aaaaagtcta agactcagga ggagcgcatg
     6361 atcgacaagc gcctcttaaa aaatgccaat attgtcttat caagtggcag gaaggcagtg
     6421 ctcgcccttt cggccc
//
LOCUS       DASRLV010000221         2549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_484794,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000221 DASRLV010000000
VERSION     DASRLV010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484794"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="VFC43_08465"
     CDS             complement(<1..960)
                     /locus_tag="VFC43_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HZK31242.1"
                     /translation="MRLLFELSGENPTLPLAELYCIGKVTDHRLQVAVVEVPDPKEAQ
                     RLAMTQVISEYLGECEPTIPAFRSLLKDLGIETDESFAGRVKKVHGGCFNKNTCSQRE
                     FEKLIGTMISGPVSLDRPAIVYRAILSEDRCYFGKVLVTIDRGTYDIRNPGKRTFFHP
                     GVMMPRMARTLINLTCSHKGDMVLDPFCGTGGILIEAGLLGMQTLGTDFDPVMVRGSL
                     QNIAKADLMVSDATHLPFKNQVMDAVVTDFPYGQSVCIKKTHSMENLYTDSLDEIHRI
                     LKAGKRAVVVTHKDISEIVPPTMAILQRHEQRVHKSLTRRILVL"
     gene            complement(1051..2175)
                     /gene="dnaJ"
                     /locus_tag="VFC43_08470"
     CDS             complement(1051..2175)
                     /gene="dnaJ"
                     /locus_tag="VFC43_08470"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HZK31243.1"
                     /translation="MSADDYYDTLGIPRSADDKEIKKAYRNLARKYHPDVCKDKGAEE
                     KFKGINEAYSVLSDEQKRAQYDNMGHETFTNASKGSYTGGGFGGGGFSTDFSGFGDIF
                     DFFGGGQRRSGPQSGADLLMRIQIRLEDAVSGVDRDIEVMHTEPCPACNGSGSETKRL
                     NTCPRCGGSGQMRQAASSAFGQFIRMTTCTQCGGKGKIPEKVCKECRGAGHSRVKRKV
                     SVHIPAGIDNGMRLRMEGYGEAGDFGAPNGDLFIEVYVQSHDRFTRSGDNLEITVEIS
                     PVQAVLGTLIEIETIDKRHIDLKIPPGIQYNTALKIAGEGMKRRGKPGDLLVRVKIVT
                     PKSISTEQRELYQKLAELEGKSAGPGGFFSGFMGKKKSKK"
     gene            complement(2184..>2549)
                     /locus_tag="VFC43_08475"
     CDS             complement(2184..>2549)
                     /locus_tag="VFC43_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZK31244.1"
                     /translation="FEADDQKKREEIELHNQADTAVFTAEKMLKESGEKLETADKLKV
                     EEGIAAVKKAIADDDLDEIKKAMESLTESVYAATTKIYQKIQAEQASQQQSGAGAAPE
                     PDKNTDDTVVNADYNVKEE"
BASE COUNT          598 a          690 c          554 g          707 t
ORIGIN      
        1 aagaacaaga atacgacggg tcagactttt atgtacccgt tgctcatggc gctgcaggat
       61 tgccattgtg ggaggaacga tctccgaaat atccttatga gtaactacca ctgccctctt
      121 tccagccttg agtatacggt gtatttcgtc cagtgaatcc gtatacaaat tttccataga
      181 gtgtgttttt ttaatacaaa cagactgtcc atagggaaaa tccgttacca ctgcatccat
      241 tacctgattc ttaaacggca gatgcgtagc atcagaaacc atgaggtccg cttttgcaat
      301 attctggagg ctgccccgaa ccatcaccgg atcaaaatca gtccccaggg tctgcatacc
      361 aagcaacccc gcttcgataa gaataccgcc ggttccacag aacggatcaa gtaccatgtc
      421 acccttgtgt gaacaggtta aattgataag cgttcttgcc atccttggca tcatcacgcc
      481 gggatggaaa aaggtgcgtt ttccaggatt gcggatgtca taggtaccac gatcgatggt
      541 gacaagtact ttgccgaaat aacatcgatc ctccgaaagg atcgcacggt aaactatcgc
      601 cggcctgtcc agggaaacgg gaccggaaat cattgtgccg ataagctttt caaattcccg
      661 ttgggaacag gtgtttttgt taaagcaccc tccatggacc tttttaacac gccccgcgaa
      721 tgactcgtca gtttcaatgc caagatcttt taagagactc cggaatgccg ggatagtggg
      781 ctcacattcg ccaaggtact cggaaataac ctgtgtcatg gcaagccgct gtgcctcttt
      841 tgggtcaggc acctcaacaa ccgcgacctg aagacggtgg tcagtaactt tcccgatgca
      901 gtaaagttca gcaaggggga gcgtcgggtt ttcaccggac aactcaaaaa gcagcctcat
      961 cctgatctca ttgatcacaa acgctcaaat aagtgcgtaa atcagtaaat caaaaccaaa
     1021 aaaaacactt ttgccatggg gtctcatata ttacttttta cttttctttt ttcccataaa
     1081 accggagaaa aagcccccgg ggccagcgct ctttccttca agttcggcaa gtttctggta
     1141 cagttctctc tgctcggtgc tgatggattt gggggtgaca atttttaccc gtaccagaag
     1201 atccccgggt tttccacggc gtttcatacc ctcgccggca attttcaatg cagtattgta
     1261 ttgaatcccg ggaggtattt tcaggtcgat atgccgtttg tcaattgttt caatttcaat
     1321 cagtgtcccc aggactgcct gaacaggact gatttcaaca gtaatctcaa ggttgtcccc
     1381 actcctggta aaacggtcgt gtgactggac atacacctca ataaacaagt caccattggg
     1441 tgctccaaaa tcaccggctt caccgtaccc ctccatgcga agcctcatcc cgttgtcaat
     1501 acccgcgggt atatgcacag acactttcct cttgactcgt gaatgacctg ccccgcggca
     1561 ctccttgcag actttttccg gaatttttcc ttttccaccg cactgggtac aggtcgtcat
     1621 ccggatgaac tgtccaaaag cggagctggc agcctgccgc atctgcccgc ttccaccgca
     1681 ccttgggcag gtattcaacc gctttgtctc actccctgat ccgttacatg caggacatgg
     1741 ttctgtgtgc attacctcaa tatcccggtc aacaccggag acggcgtctt cgaggcggat
     1801 ctggatccgc atcagcagat cagcaccgga ttgagggccc gatcgtctct gacccccgcc
     1861 aaagaaatca aagatatctc cgaaacccga gaaatcggtg gagaatccgc cacccccgaa
     1921 ccctcctccg gtgtacgagc ctttggatgc gttggtaaac gtttcatggc ccatattatc
     1981 atattgtgct cttttctgtt catcggaaag aacactatat gcctcgttga tccccttgaa
     2041 tttctcttca gcacccttat ctttgcacac atcggggtga tatttcctgg caaggttgcg
     2101 gtatgccttc ttgatctcct tatcgtcagc actacggggt attccgagag tatcgtagta
     2161 gtcatccgca ctcatttttt agctcactct tcttttacgt tataatctgc attaacaacg
     2221 gtgtcatcgg tgtttttatc aggctctggt gcagcgccag ctcctgactg ctgttgtgaa
     2281 gcctgttccg cctggatctt ctggtagatt ttggttgttg ctgcatagac actttcagtc
     2341 agcgattcca tcgctttctt aatctcatcg aggtcatcgt cagctatcgc tttctttact
     2401 gcagcaatac cttcctcaac ttttagcttg tcagctgttt caagcttctc cccgctctct
     2461 ttaagcatct tttcagcagt gaatacggca gtatccgcct ggttgtgcag ctcgatttcc
     2521 tcacgctttt tctgatcgtc agcctcgaa
//
LOCUS       DASRLV010000222         5841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_1054619,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000222 DASRLV010000000
VERSION     DASRLV010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054619"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /locus_tag="VFC43_08480"
     CDS             complement(<1..1334)
                     /locus_tag="VFC43_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZK31245.1"
                     /translation="MVEGSYASGTSSFPLLGMTIGEMVNSIAEKYPDNEAIVSVHQDI
                     RWTYREFLERVNQVARGLMGLGVEKGDRVGIWAMNHAEWVVVQFATAKIGAIMVNINP
                     AYRTYELEYALKQSEIRTLIIQGRFKTSDYVGMFYEACPEAYEAKPGRISSDKFPFLK
                     NAIFLGNIPYNGMFTWDEMLKKAEEVTNDELIERGDGLTFDDAINIQYTSGTTGFPKG
                     VVLTHHGVLNNGYIIGEGMGFTEKDRLCIPVPFYHCFGMVLSNLACVTHGSTMVLPAP
                     TFDAEEVLKTVEKERCTALHGVPTMFIAELTHPNFEKYNLKSLRTGIMAGSPCPIEVM
                     KQVNTKMHMRDIVIVYGQTETSPGVTMTTTKDPVERRVSTVGRAFPHTELKIVDSKNG
                     KIVPMGEIGEICARGYCVMKCYYNNPSATHATLDSNNWNHTGDLGTMDEEGY"
     gene            1550..1772
                     /locus_tag="VFC43_08485"
     tRNA            join(1550..1587,1737..1772)
                     /locus_tag="VFC43_08485"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1584..1586,aa:Trp,seq:cca)
     gene            2072..3253
                     /locus_tag="VFC43_08490"
     CDS             2072..3253
                     /locus_tag="VFC43_08490"
                     /EC_number="4.1.2.43"
                     /EC_number="4.2.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,6,7,8-tetrahydromethanopterin
                     hydro-lyase/3-hexulose-6-phosphate synthase"
                     /protein_id="HZK31246.1"
                     /translation="MYLIGEALIGDGAEVAHIDLLMGDKEGPIGTAFAHAVSQLSAGH
                     TPLLAVIRPNLLTKPVTLVIPKVTLKTMTQVNEMFGPVQAAVAKAVADCVEEGVFAAV
                     DIENVVILASAFVHPDALDYNKIYRYNYGATKLAIHRAIDKFPDAKTLVYEKDRAAHA
                     IMGFKVTRLWDPPYLQVALDLVDMGKVATVLKELPENDHLIIEAGTPLIKKFGLGVIS
                     ELRKLRPNAFIIADMKILDTGNLEARMAADASADAVVVSGLAPASTMEKAIAEARKVG
                     IYSVVDMLNVQNPAKVIEKLKNKPDIVELHRAIDTEDTAHAWGDIAAIKKAAGGKLLV
                     ATAGGVRVNVVQDALKAGADILVVGRAITASKDIKHSADEFLDKLDKEEIDQFRVMTD
                     F"
     gene            complement(3752..4330)
                     /locus_tag="VFC43_08495"
     CDS             complement(3752..4330)
                     /locus_tag="VFC43_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZK31247.1"
                     /translation="MPRIIQEYRDEVRKKIVEAAYGLFLQKGYHATTMSAIAETLGVT
                     KPALYQYFPGKEELYAAVAEHGREELAAILERSFNKRDLRSGSEILFDTLARYTPQFN
                     GIYSEMMMLAMHNEQIRNLLHADRIEDIRVVERFITRQQETGLVSGQLNPRVLAIACD
                     ALMNGLLIDIMAGMDKEEAKKIWIAAVTRLTE"
     gene            4463..5806
                     /locus_tag="VFC43_08500"
     CDS             4463..5806
                     /locus_tag="VFC43_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31248.1"
                     /translation="MSGWMYSMSATSLSYREKTNGECTGKWAKYLQFISCVFVLVFLS
                     SIVMPVSGAGEPTVTITDYKVTPSVLMPDGLGTITIMVKNTATSATIKENTGLYTTDA
                     TTTRVTDINVNIENVKLEGNGIIVQSKDFQQVGEIGPGQSIQLTFSIQAPSKSGMYFP
                     EVWIDTTGGRSTRYPVPVNVNTAVGIQRQAILIMGSTLTGSVNPGDEIPVTLTVSNAG
                     QTLADDVTLRIDNVSGTVAPKNADLYHMGTIAAGGQKTVDLSLLSDKKSTSGLVRIPV
                     LISYNAIDGIQQTQDTGIDVIMKGKAELGFVSVDTNPPRLTENTPFDLTVRIENTGSG
                     EAKQLSAKIDLPAEGTREAFIGKIKPGNDAPAIFLLEGLKGGNYPYNLTVTYTDDMGA
                     HTFSRPMNMRVPPADMSGTIILGLIVLVILGVIGYRYWYLPKVNGDGKFPWDKKN"
BASE COUNT         1457 a         1479 c         1462 g         1443 t
ORIGIN      
        1 aagtaccctt cttcgtccat ggtcccgagg tctccggtgt ggttccagtt gtttgagtca
       61 agtgttgcat gagttgcaga cgggttattg taataacatt tcatcacgca gtaaccgcgg
      121 gcgcagatct ccccgatctc acccataggg actattttac cgttcttgga atcgacaatc
      181 ttgagttcag tatgcgggaa tgcacgcccg accgtcgaga cacgtcgctc aaccgggtcc
      241 ttggttgtgg tcatggtaac tcccggtgat gtttcggtct gtccatacac gattacaatg
      301 tcacgcatgt gcatctttgt gttgacctgc ttcatcactt caatcggaca gggtgatccc
      361 gccataatgc cggtacgtaa agacttcagg ttgtattttt caaaattcgg gtgggtcagt
      421 tcagcaatga acattgttgg cacgccatga agagccgtgc accgctcttt ttcaacagtt
      481 tttaagactt cctcggcatc gaacgtggga gcaggcagga ccattgttga accgtgcgtc
      541 acacacgcga ggttggagag gaccatacca aagcagtgat agaacggcac gggtatgcaa
      601 agccggtctt tttcagtaaa gcccatcccc tctccgataa tataaccgtt gttaaggaca
      661 ccatgatggg taagaacaac tcctttggga aaccctgtcg tgccgctggt gtactggatg
      721 ttgatagcat catcgaaggt aagcccgtca cctctctcga taagctcatc gtttgtcact
      781 tcttcagctt ttttcagcat ctcatcccac gtgaacatgc cattgtaagg gatattaccc
      841 aggaagatgg catttttcag gaaagggaac ttgtcagatg agatcctgcc tggtttcgct
      901 tcataagctt ccgggcatgc ctcgtagaac atccctacgt aatctgaggt cttgaaccgt
      961 ccctgaataa taagtgtccg gatttctgat tgtttgaggg cgtactccag ctcgtaggtt
     1021 cggtatgccg ggttgatgtt caccattata gcgccgatct tcgcagttgc gaactgaaca
     1081 acgacccatt ccgcatggtt catggcccag ataccaacac ggtccccctt ttccacgcca
     1141 agccccatca gcccgcgggc gacctggttt acccgctcga gaaattcgcg gtacgtccag
     1201 cggatatcct ggtgcaccga gacaatcgcc tcgttgtccg ggtatttttc cgcaatactg
     1261 ttcaccatct cgcctatcgt cattcccagg agaggaaatg acgaagtccc gctcgcgtag
     1321 ctgccctcga ccatccttaa aatatggggt gagaacagga tatatgatta atgatgtgat
     1381 gtgtcctttt ccctttttaa gatcctgagc ggaaaaaaga aggttgtgga aaaaaccggc
     1441 cacctatcag gaaaatttct ggcaaatgag gtttaaaagg aaaactatta aattaattta
     1501 acgcgatttt gtacagtcag ctatgggaaa actatcccgg catgaccgag gggttgtggc
     1561 ctagcccgga atggcgacgg gctccagcgg tctttgcgtt ctttgtgctg cagatgatga
     1621 acaatgggtc tcctgaattc atcttcctgg cctatgccag ggggagcacc agtaacttaa
     1681 tactaagttc tggtgtttga tgatgacccg ttggagcact gatgcatgtc ggagagaccc
     1741 gtcgatcgtg agttcaaatc tcaccaaccc cacattttct tatcatgcag attcctgatc
     1801 ctgacacttg tcactcgtga tcaatggtct cttaacttcc cgtgtatcta aaacctcatg
     1861 gtgtgcaacg gggtatttat tcctgaaacg tgttttgctc tcactcttgc ttacacagga
     1921 gatttggcga cgaatcagca aaggaacaat ccgccgggag aagaattccc gtgggtatat
     1981 ccggttgttt tttcttgagc ctgataaccc gcttcgtttg ataaatttat catagctgac
     2041 agacacataa taacaacgaa cggagagttc catgtatctt attggagaag cacttatcgg
     2101 tgatggtgca gaagttgcac acattgacct tttaatgggg gacaaggaag gtcccatcgg
     2161 gaccgcattt gctcatgcgg tatcccagtt gtctgccggg catacaccgc tccttgctgt
     2221 tattcgcccc aacctgctca caaagcccgt gaccctcgtt attccgaagg tcacgttaaa
     2281 gacgatgacg caggtcaacg agatgtttgg cccggtccag gcagccgttg caaaagcggt
     2341 tgcggactgc gtggaagaag gtgtttttgc cgctgtggat attgaaaacg tcgtgatcct
     2401 ggcctctgca tttgttcacc ccgatgcact ggactataac aagatttacc ggtacaacta
     2461 cggtgcaacc aaacttgcta tccaccgggc aatcgacaaa ttccccgacg caaagaccct
     2521 tgtctatgaa aaagaccggg ctgcccatgc aattatggga ttcaaggtca cccggctctg
     2581 ggacccgccc tacctgcagg tagctcttga ccttgtcgat atgggcaagg tggcaacagt
     2641 gttgaaggaa ctcccggaaa acgaccacct tattatcgag gccggcaccc cgctgatcaa
     2701 gaagttcggt ctaggcgtca tttccgagct gcggaagctg cggccgaatg ccttcatcat
     2761 cgccgatatg aagatcctgg acaccggcaa cctcgaagca aggatggcgg cagatgcatc
     2821 agcggatgca gtcgtcgtat caggccttgc cccggcctcg accatggaaa aagccattgc
     2881 tgaagctcgt aaggtaggca tctactcggt agtcgacatg ctcaatgtcc agaacccggc
     2941 aaaagtcatt gagaagctga aaaacaagcc ggacattgtg gaactccacc gtgccatcga
     3001 caccgaagac actgcacatg cctggggcga tatcgctgca atcaagaaag cagccggcgg
     3061 taagctcctt gtcgcaacag ccggtggtgt ccgggtcaat gtagtccagg acgcactgaa
     3121 agcgggagca gatattctcg ttgtcggccg tgcaatcacc gcaagcaagg acatcaagca
     3181 ctcggcagat gagttcctgg acaaactcga taaggaagag atcgaccagt tcagggttat
     3241 gactgatttt taaatatcat aatgtatatt ttttttgctc tgtagaaaca ggcatgaacg
     3301 ggaattttca cccgaaccat gaaaatcgta caatatacga tgcaccccgc ctcagggcct
     3361 ctccgaggca cggcgggacg tctcggctgt ttaaaatttt tgttattgat ccgccgcggg
     3421 ggcgccccgt cgggggcggc ggcgttcatc gtaactgaag aatagatctg ataatcccct
     3481 catcatacct gaatgaattg atcttcaggg gaaatccttt gaataattct gatgctcttg
     3541 ggggcatgca agcatgcccc cgccgctgtg gcatcccctg attgcgatta tgccatatta
     3601 cctgtacgac ctgtacgcag gtgtcgaaaa atttctagtt ttggtaatgc ggaggcatcg
     3661 caatgcgccc ccgcccccaa tgggggcagg gtcggcgcaa ggggggcgat cataacttga
     3721 aaagaggatt tctaccgtgc caaaaaatca attattcagt caatcgggtg acagctgcaa
     3781 tccagatctt ttttgcctct tctttatcca ttcctgccat gatatcgata agaaggccgt
     3841 tcataagagc atcgcaggca attgcaagta cgcgggggtt gagctgacct gataccagtc
     3901 ctgtttcctg ctgccgtgta ataaatcgtt caacaacgcg gatatcttcg attcgatctg
     3961 catggagcag gttgcggatc tgttcattat gcatggcgag catcatcatc tctgaataga
     4021 taccgttgaa ctgcggcgta taccgtgcaa gagtatcgaa aaggatctca ctgccgctcc
     4081 gtaaatccct tttattgaag gaccgttcca ggattgcggc gagttcctcg cgaccatgtt
     4141 cggcgacggc agcgtaaagt tcctcctttc cgggaaaata ctggtacagt gccggcttgg
     4201 taacgccgag agtttcagca atcgcgctca tggttgtggc atgataccct ttctggagaa
     4261 agagaccata cgctgcctcg acgatttttt ttctgacttc atccctgtat tcctggataa
     4321 tgcgtggcat gggaacccct ccttattgcg cgtttttata aaaaaatttc cctttgggga
     4381 atataccata ggtattatta ggttacccat ggtaagttta ataactttgg gtagagcacc
     4441 tgagatataa caagaccggc gtatgtccgg ttggatgtat tccatgtcag caacatcatt
     4501 atcctataga gaaaagacga acggagaatg tacgggaaaa tgggcgaaat acctgcagtt
     4561 catttcatgc gtattcgttc tcgttttcct ctcgtccatt gtcatgccgg tttctggcgc
     4621 cggagaacct acggtaacaa tcaccgacta caaggtcacg ccctctgtgc ttatgccaga
     4681 tggtctcggg accatcacca taatggtcaa aaataccgca acgagtgcaa cgatcaagga
     4741 aaatactgga ttatatacaa cagatgctac gaccaccaga gtcacggaca tcaatgtcaa
     4801 tatcgaaaat gtaaaacttg aaggcaacgg gatcatcgtc cagagcaaag atttccagca
     4861 ggtgggcgag ataggaccgg gacaatccat ccagttgaca ttttctatcc aggccccctc
     4921 aaaaagcggg atgtattttc ctgaagtctg gattgatacc accggaggaa ggagtacccg
     4981 ctatcctgtt ccggtcaacg tcaacaccgc agtgggaatc cagcgacagg ctatccttat
     5041 catgggcagc acactgacgg gcagtgtaaa tcccggggac gagatcccgg tcacactcac
     5101 ggtcagcaat gccggccaga cccttgccga tgatgttaca ctccggatcg ataacgtaag
     5161 cgggacggtc gccccgaaga atgcggatct gtatcacatg gggacgatcg cggctggcgg
     5221 acagaagacg gttgacctgt ctttattatc ggataaaaag agcacttccg gtctggtcag
     5281 gatccccgtt ctcatcagtt ataatgcaat agacggcatt cagcagaccc aggacacggg
     5341 cattgatgta ataatgaaag gaaaggcgga attagggttt gtttccgtgg ataccaatcc
     5401 ccccaggctc accgagaata ccccgtttga ccttaccgtc cggatagaga ataccggttc
     5461 aggcgaagca aaacaactgt cagcgaagat cgatcttccg gcagaaggga cacgtgaggc
     5521 attcatcggg aagatcaagc cgggcaatga tgccccggcc atattccttc ttgaaggatt
     5581 gaaaggaggc aattacccgt acaacctgac cgtcacctat acagatgaca tgggtgccca
     5641 taccttctca cggccgatga atatgcgggt accccccgct gacatgtcag gcacgattat
     5701 cctcgggctg atcgtcctcg ttattctcgg tgttatcggc taccggtact ggtacctccc
     5761 gaaggtcaat ggtgacggga agttcccatg ggacaaaaag aactaaaggt cgccttcctg
     5821 cttgccctga ggtcactcca g
//
LOCUS       DASRLV010000223         6880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_78448,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000223 DASRLV010000000
VERSION     DASRLV010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 6880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6880
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78448"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VFC43_08505"
     CDS             complement(<1..451)
                     /locus_tag="VFC43_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZK31249.1"
                     /translation="MQWTDLKARLLAVGQVHLTGEPAENYIARSAAGPGAGGSGAVFF
                     AMGSQRVKLAISPVSSIEIAHRGNGTADLFFEGQRIPGYLLEPGFHCPDQAFITVTGS
                     CIFSCSYCSVPRIGGKRKTIKEITEMVESVHDHIHAISITSGVLTSIE"
     gene            complement(433..1329)
                     /gene="mmp11"
                     /locus_tag="VFC43_08510"
     CDS             complement(433..1329)
                     /gene="mmp11"
                     /locus_tag="VFC43_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker protein 11"
                     /protein_id="HZK31250.1"
                     /translation="MVTITEPYIIHYPQIVAVADESGGHVELIEFFDCVGGAMWSKHH
                     YAQSPVVKNVRCVGRTMRYLVTPGTVDLALEGSRFPAGISSCMVNTSEIAITYIGMGG
                     GGVGAAACRTDARGVLRSRSDESGGGKVAGSTVWLPRMQRVLIGVDDTDTPDEGATWT
                     LVHNIAKAVEDEHSVYLSHTIVQLFPVPYRTKNCVSIVVEFATTDTRGLTERFRQLLE
                     RYTLSQKTGMAVYTGFTPSEELVAYGRNVKRGEIKPGVLRDLDDKNLQIILDGRGIIG
                     AVAAIPFYTRYEEALELCSGRT"
     gene            complement(1502..2386)
                     /gene="fhcD"
                     /locus_tag="VFC43_08515"
     CDS             complement(1502..2386)
                     /gene="fhcD"
                     /locus_tag="VFC43_08515"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HZK31251.1"
                     /translation="MEINGVTIDNTYAEAFPTWVCRVIITAVTKDWARKAATEATGFA
                     TSAIGCPCEAGIEGDIPASETPDGRPGVAILICASKKKLKEQVVERLAECVLTAPTTA
                     VFNGITTAEEKIQVKLHFFGDGYEYQKEVGGRKCWVIPIMQGEYVGEEEFGIVKGVAG
                     GNFFVMAENQMAALVGAQAAVEAIDKVPGTITSFPGGIVASGSKVGSLKYKFMPASTN
                     EKYCPTLREKVPDSKIPAGIKAVYEIVIDGIDEKSVAAAMGAGVKAAVMVPGVKFISA
                     GNFGGTLGPFKIELHKVL"
     gene            2651..2917
                     /locus_tag="VFC43_08520"
     CDS             2651..2917
                     /locus_tag="VFC43_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZK31252.1"
                     /translation="MAFAVHINMENCTGCNNCVVACPVDALELHTVDPSSKDKIYKVK
                     DGKSIVLDFKGELCAGCGVCVQACPHDVIKLAGPWESRAQAKVQ"
     gene            2941..4131
                     /locus_tag="VFC43_08525"
     CDS             2941..4131
                     /locus_tag="VFC43_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZK31253.1"
                     /translation="MALFPKFSKKREGVNVVMEQRLLQSVNNLILNSETCTGCGICVE
                     SCPEDAIVLGLVGASRRGAINYAAPVDVDEVKCSYCGVCVVMCPFNALTLKVDGLEKL
                     PILEKEGFPQYDMKAEIDDEKCTRCTICEEVCPRDAIDRNVPAYEGTYKGPVTGAKDR
                     QTAIKTKTTFKVDMEKCSLCGICGALCPAITVKHKEFTAETGKVETVADPVTGLPWDE
                     TKCDACKVCVEACPEECITVEREVISDKIEGKVSIIQDNCCTCQWCSRNCPPEAITVE
                     KIFEGDIEFHAEKCPGGCSTCADICPANAIYLPSSKPAADMKGEVEKTIAVNKDYCIL
                     CGACVNACPGEDIIVMQRTGVRMKGTETDLFKKIKEKLFTKRTSKVKEEVTGQVNLKV
                     MEKA"
     gene            4141..4722
                     /gene="hdrC"
                     /locus_tag="VFC43_08530"
     CDS             4141..4722
                     /gene="hdrC"
                     /locus_tag="VFC43_08530"
                     /EC_number="1.8.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase subunit C"
                     /protein_id="HZK31254.1"
                     /translation="MVRTKGYPEALEKKLRDQRYYSEDSNPEFTKNVKGISRTIAHMC
                     YQCGTCTGSCPSAPRSTYRIRKFMRRAVLGLENESLTDPDLWLCTTCYSCTDRCPRDI
                     APTDVIMAMRNLAFKRDIIPVNFLKTVQAIYSSGHGVPNNDVNRAARERIGLSRDPPT
                     THMYPEYVKGIQKILDHYKLKANADRIVKEREG"
     gene            4729..5646
                     /gene="hdrB"
                     /locus_tag="VFC43_08535"
     CDS             4729..5646
                     /gene="hdrB"
                     /locus_tag="VFC43_08535"
                     /EC_number="1.8.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase subunit B"
                     /protein_id="HZK31255.1"
                     /translation="MSEAKHKFAFYLGCIAPNRYPGVESASIKAMKKLGVELVPLKGA
                     SCCPAPGAFGSIDLNVFYAMAARNLVLAEQMKMDIALVCNGCYKSIWEVNHKLKHNKD
                     LRDGVNEVLKEIDMEYKGTINVYHTAELLYNDKFIGVEKVRASVTNPLTGARIAVHYG
                     CHLTKPHKDREFEDEVMLNTEHPTWMEELVAAIGATPVEYRNKMQCCGAGGGVRGYDI
                     VHALDIANEKLINLKEVGVDALTDICPFCQLQFDRGQIEIKEKFGVSYDLPVLHFAEL
                     LGLAQGMSPQDLGLDLHGISCESFLQKVL"
     gene            5657..>6880
                     /locus_tag="VFC43_08540"
     CDS             5657..>6880
                     /locus_tag="VFC43_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK31256.1"
                     /translation="MAEKKKTTTKKTEAKKQEAPAKQEAKKQAAPSVGSTSKEQPRIG
                     VFICHCGTNIAGSMDITAVQEYAKTIPNVGYVDNYKYMCSMPGQAVINKAIKDNKLTG
                     VVVAACSPRLHEPTFRTATKEGGLNQFRFEMANIREQNSWVHMHDQEGSTEKAKDAIR
                     IAVAKASLLQDLFPKAVPVEHAAMVVGAGVAGMQAALDLANAGIKTYLIESQPTIGGR
                     MSQLDKTFPTLDCSQCILTPKMVDVGRAANIELMTYSEVDAVEGYIGNFDVTIRKKAR
                     GVLTPAEAEARGIVGGGCNGCGDCEAPCPVIKPNEFEVGMKPRKAIYISHPQVVPLIY
                     TIDWDSCIKCGLCVTACGPEKRAIDLEAKDEFVKVKVGTAILATGYDMFPVEKKTEWG
                     YKKFENVINSLEFERL"
BASE COUNT         1823 a         1756 c         1718 g         1583 t
ORIGIN      
        1 cttcgatact cgtgagaaca ccgcttgtta tggagattgc atggatatgg tcatgtaccg
       61 attccaccat ctctgtgatc tctttgattg ttttgcgttt tcccccgatc cgggggactg
      121 aacaatagct gcaggagaaa atgcagctgc cggtaaccgt gataaaagcc tgatcagggc
      181 aatggaaccc gggttccagc aggtacccgg ggattcgttg tccttcaaag aacagatcgg
      241 cagtcccgtt gccgcggtgt gcaatttcga tgctgcttac cgggctgatc gcaagcttga
      301 ccctttggct tcccatggca aaaaaaaccg caccgctgcc accggcacca ggtcctgccg
      361 ctgaccgggc gatatagttt tctgccggtt caccggtcag atgtacctgg ccaacggcca
      421 gaagccgggc cttcaggtcc gtccactgca taattccagt gcctcctcgt acctggtgta
      481 aaacgggatg gcagcaaccg ctcctattat accccggccg tccaggatta tctgtaggtt
      541 tttgtcatcc agatccctga gaactcccgg tttaatctcc ccccgtttga catttcgccc
      601 gtatgcaacc agttcttctg acggggtaaa ccctgtatag accgccattc ccgttttttg
      661 ggagagggtg taccgttcga ggagttggcg gaacctttct gttagtccac gggtgtcggt
      721 agtcgcaaac tcaaccacaa tactcacaca gttctttgta cggtacggta ccgggaagag
      781 ctggacgatc gtgtgcgaga gatataccga atgttcatcc tcaaccgcct ttgcaatatt
      841 atggacaagc gtccacgtgg caccctcatc aggggtgtcc gtatcatcga cgccgataag
      901 aacccgctgc atgcgaggca gccataccgt ggaaccggca actttcccac ccccggattc
      961 atcgctcctg ctccgcagga caccccgtgc atcagtccgg caggcagcag cgcctacccc
     1021 accaccaccc atgccgatgt aagtgattgc gatctcagag gtatttacca tgcatgagga
     1081 gataccggcc ggaaaccggg atccttccag tgcaagatcc acggtgcccg gggttaccag
     1141 gtaccgcatg gttcggccga cacagcggac attttttacc accgggcttt gagcatagtg
     1201 atgctttgac cacattgccc cgccaacaca gtcaaaaaac tcaatgagct ccacatgacc
     1261 acccgattca tcagctactg caacgatctg cggataatga ataatgtacg gttcagtgat
     1321 ggttaccata gtgtcctgcc ctggctgtcc cgagagtaat ccccaccaga ctcgcccttg
     1381 gttcacctta tatctgggtg atcatgttat agccgaaaat ttcgttaaca aaagaatcgg
     1441 cgataaaaat ataaataacg atcgatacga ttttaaaaaa tcttaaaaaa aatggaaaag
     1501 ttcaaagaac tttgtgcagc tcgatcttga aaggtccaag cgtaccgcca aagttcccag
     1561 cagagataaa cttcacacct ggtaccatga cagctgcctt gacaccggcc cccattgctg
     1621 ctgcaacgga cttctcgtca atcccgtcaa taacaatctc atagacagcc ttgattcctg
     1681 ccgggatttt actgtcagga actttttccc gcagagtcgg gcagtatttt tcattggttg
     1741 aagccggcat aaacttgtac ttgagactcc caaccttcga gccactggca acgatccctc
     1801 cagggaaact cgtgatcgtc ccggggactt tatcaatggc ttcaacagct gcctgtgcac
     1861 caacgagggc tgccatctgg ttctctgcca tgacaaagaa gttacccccc gctacacctt
     1921 ttacaatacc aaattcttct tctccaacgt attcaccctg catgatcggg ataacccagc
     1981 attttcttcc accaacttcc ttctggtatt cataaccatc cccaaagaag tgaagcttga
     2041 cctgaatctt ctcttctgca gtcgtgatac cgttaaaaac ggctgtcgtc ggagcagtaa
     2101 gaacgcattc cgcaagacgt tcgacaacct gttccttgag tttcttttta cttgcgcaaa
     2161 tcaaaattgc cacaccgggc cttccgtcag gagtctcact cgcagggatg tctccttcaa
     2221 taccagcctc gcacgggcaa ccgatcgcag atgtcgcaaa cccggtcgct tcggttgctg
     2281 ccttcctcgc ccaatccttt gtaaccgcag tgatgataac acgacatacc catgtcggaa
     2341 atgcctctgc gtaggtatta tcaatagtaa ccccgttgat ttccatatac taacctccgg
     2401 tactacaaag gtacaatgat ggtgattgaa taaattttct aaatgaattc agctttatca
     2461 agcgcataaa aaatacctga ttaagtaaat tttttaggat taacttattt gtttagttta
     2521 gatattgaaa ttaaaattct tcaataaata agcctttttc tggcgtcaat ttcagaacat
     2581 ttatctatat tcttccccca ctttttgata gtcgattttt gtaatcgatc atagctggtg
     2641 cgtgaaaaaa atggcatttg cagtgcacat aaacatggaa aattgtaccg gctgtaacaa
     2701 ttgtgtcgtc gcgtgccctg tggacgcatt ggagcttcac actgtagatc catcctctaa
     2761 agataagatc tacaaagtca aggatggaaa atccattgtg cttgatttta aaggtgaact
     2821 ctgcgccggt tgcggcgttt gtgtccaggc atgtccgcat gatgttatca agctggcagg
     2881 gccctgggag tccagagcac aggcaaaggt acaataaagg aacaacgtga gggttatttc
     2941 atggcattgt ttccaaagtt ttctaaaaaa cgagagggtg taaatgttgt gatggagcag
     3001 cgcctccttc agagcgtgaa caacctcata ctgaattcag agacctgcac aggatgtggt
     3061 atctgtgtcg agtcttgtcc cgaggacgca atcgtgctcg ggctcgtcgg tgcatcaagg
     3121 cgtggcgcta tcaattacgc tgccccggtg gatgttgacg aggtcaaatg ctcgtattgt
     3181 ggtgtttgcg tcgtcatgtg cccgtttaat gcattgaccc tgaaggttga tggactggag
     3241 aaacttccga tccttgaaaa ggaagggttc ccgcaatatg atatgaaggc ggagattgat
     3301 gacgagaaat gcacccggtg cacgatctgt gaagaggtct gcccaaggga tgcaattgac
     3361 cggaacgtcc ccgcgtacga aggaacctac aagggaccgg ttaccggagc aaaggacaga
     3421 cagacggcga tcaaaaccaa gaccaccttt aaagtcgata tggaaaaatg ttcgctgtgc
     3481 ggtatttgtg gcgcactctg tccggcaata acggtcaaac acaaggagtt caccgcagaa
     3541 accggaaagg tcgagacagt cgccgacccg gtcaccggac tgccgtggga cgagaccaaa
     3601 tgtgatgcct gcaaggtgtg tgtagaagca tgtcctgaag agtgcatcac cgtcgaacgc
     3661 gaggttatca gcgataaaat tgaaggtaaa gtcagcatta tccaggacaa ctgctgcacc
     3721 tgccagtggt gttcacggaa ctgcccgcct gaggcaataa cggtagagaa gatttttgaa
     3781 ggcgatattg aattccacgc tgagaaatgc cccgggggtt gctcaacatg tgcagatatc
     3841 tgcccggcta atgcgatcta tctcccgtca tctaaacctg cagccgacat gaaaggcgag
     3901 gtagagaaga ccattgccgt caacaaggat tactgtatcc tgtgtggcgc ctgtgtcaat
     3961 gcatgccccg gtgaagatat catcgtcatg cagaggaccg gtgtccgcat gaagggcacg
     4021 gagacagacc tgttcaagaa aataaaagag aaactcttca caaaaagaac ttcgaaagtg
     4081 aaagaggaag tgaccggaca ggtcaatctc aaagtgatgg agaaggcgtg agggagtact
     4141 atggttagaa caaaaggtta cccggaagcg ttggaaaaga aactccgcga ccaaagatac
     4201 tacagtgagg actcaaaccc tgagttcacg aaaaacgtga aaggaatctc acgcactatc
     4261 gcccatatgt gctaccagtg cggaacctgt accggatcgt gcccgtcagc tcccagaagc
     4321 acgtaccgca tccgtaaatt catgaggcgg gccgtgctcg gtctcgagaa cgagtccctc
     4381 acggatccgg atctctggct ctgtactaca tgctatagct gtactgaccg atgtccacgt
     4441 gatatcgcac ctacagatgt cataatggcc atgaggaacc tcgcattcaa gagagatatt
     4501 atacccgtga acttcctcaa aaccgtgcag gcaatttaca gctcaggaca cggggtcccc
     4561 aacaatgatg tcaaccgtgc tgccagagag aggatcgggc tttcccgcga cccaccgaca
     4621 acccacatgt atccggaata tgtcaagggt atccagaaga tcctggacca ctacaaactc
     4681 aaggcgaatg ctgaccggat tgtcaaggaa agggaggggt aagataccat gtcagaagca
     4741 aaacataagt tcgcattcta tctcggatgc attgccccca accgttaccc gggtgttgaa
     4801 tcagcttcta ttaaagcgat gaaaaaactc ggtgttgagc tggtccctct caagggtgca
     4861 agctgttgcc ctgccccagg tgcattcgga tcgatcgatc tcaatgtatt ttacgcaatg
     4921 gcagcacgca accttgtcct cgcagaacag atgaaaatgg atattgctct cgtctgcaac
     4981 ggctgctata agtcgatttg ggaagtcaac cataagctca agcacaacaa agatctccgt
     5041 gatggcgtta acgaagtgct caaagagatc gatatggagt acaagggcac catcaatgtc
     5101 taccataccg ctgaacttct ctacaatgat aaattcattg gtgtcgagaa agtccgggcc
     5161 agtgtgacca acccgctcac gggtgcacgt atcgctgtcc actatggctg tcacctgacc
     5221 aagccccata aagaccggga gtttgaagat gaagtcatgc tgaacaccga gcacccgacc
     5281 tggatggaag aactggttgc cgcaataggt gcaacaccgg tcgaataccg gaacaagatg
     5341 cagtgctgtg gtgccggtgg tggtgtccgt ggctatgata tagtacatgc gctggatatc
     5401 gccaatgaga aactgatcaa cctcaaagaa gttggtgttg atgctctcac ggatatctgc
     5461 ccgttctgtc agctccagtt tgaccgcggc cagattgaga ttaaggagaa gtttggtgtg
     5521 tcatacgatt tacccgtact gcactttgct gaactgctcg gactggcaca gggaatgagt
     5581 ccgcaggacc tcgggctgga cctccacggg atcagctgcg agtcgttcct gcagaaggtg
     5641 ctgtgaggtg ggaataatgg ctgaaaagaa gaaaacaact accaaaaaga ctgaagcaaa
     5701 gaaacaggaa gcccccgcaa aacaggaagc caaaaagcag gcagccccat cagttggcag
     5761 tacctctaaa gaacagccac gtattggtgt cttcatctgt cactgcggaa caaacattgc
     5821 tggttcaatg gatatcaccg cagtacagga atatgcaaaa accattccga atgttggcta
     5881 tgttgacaac tacaagtata tgtgctcgat gcccgggcag gctgtgatta acaaggcaat
     5941 caaggacaac aaacttaccg gagtcgttgt cgcagcatgt tcaccgcgtc ttcacgaacc
     6001 aacgttcagg acggcaacca aggaaggcgg cctgaaccag ttccggtttg agatggcaaa
     6061 catccgtgag cagaactcat gggtgcacat gcatgaccag gaaggatcaa ccgagaaggc
     6121 aaaagacgcg atccgcattg ctgttgcaaa ggcctcactc cttcaggacc tgttcccgaa
     6181 ggctgttccg gtcgagcacg cggcgatggt cgtcggcgcc ggcgttgccg gtatgcaggc
     6241 tgcgctcgac cttgcaaacg caggtatcaa gacctacctt attgagtccc agcctaccat
     6301 tggcgggcgc atgtcccagc tggacaagac attcccgacc ctcgactgct cgcagtgtat
     6361 cttaacacca aagatggtcg atgtgggcag ggccgcaaat atcgagctga tgacctactc
     6421 cgaggttgat gcagtcgagg gttacattgg taacttcgat gtaaccatcc gcaagaaggc
     6481 acgcggagtg ctcacccccg ctgaggcaga agcacgcggt attgtcggtg ggggatgtaa
     6541 cggttgtggt gactgtgaag caccatgccc ggttatcaaa cccaacgagt tcgaagtagg
     6601 catgaagccc agaaaggcga tctatatcag ccacccacag gttgtcccgc taatctacac
     6661 tattgactgg gattcctgta tcaagtgcgg tctgtgtgtt actgcctgtg gtcctgagaa
     6721 aagggccatt gatcttgagg ccaaggacga atttgtcaag gtcaaagtcg gtacggcaat
     6781 tctcgcaacc gggtacgaca tgttccccgt cgagaagaaa acagaatggg gatacaagaa
     6841 gtttgaaaat gttatcaaca gcctcgagtt cgagcggctc
//
LOCUS       DASRLV010000224         5401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_730527,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000224 DASRLV010000000
VERSION     DASRLV010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730527"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VFC43_08545"
     CDS             complement(<1..711)
                     /locus_tag="VFC43_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HZK31257.1"
                     /translation="MSFTAPEELKTTSVPPDNIWKCHVCGFISRGDNPPQECPQCAST
                     FREFEEISLKKKLRYDGVKFDVLLINGSNHRANNTGYMVDLIEEVLKEKGVSYRRYNL
                     NEFAINHCWCCYSMRDNACRYPCRDQLDDMPAFHEMIIASKAVIVGSPINWNNMSARL
                     KDFLERLNCIQNLYLLKKPGLTEGKIVGITVSGHEDGATKTAMDIFLYFQQMGYVLAP
                     FGVSYRTHGAQFNTITDTE"
     gene            complement(659..886)
                     /locus_tag="VFC43_08550"
     CDS             complement(659..886)
                     /locus_tag="VFC43_08550"
                     /inference="COORDINATES: protein motif:HMM:NF022420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2769 domain-containing protein"
                     /protein_id="HZK31258.1"
                     /translation="MERKEAITLCICKICPTFIDCGEEIAFCLAARGKSTCITNEDGC
                     LCPGCPVLESEGFSHVFYCTRGTENDQRSAR"
     gene            complement(966..1370)
                     /locus_tag="VFC43_08555"
     CDS             complement(966..1370)
                     /locus_tag="VFC43_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HZK31259.1"
                     /translation="MGHSLHTYPWYVRCIISWYCKTIPHRLLGLKSRMTVKKIGTEKN
                     FAYMPMKEIEADGNDILVVKTGKIFTAIGNTCTHMGCKLSGGKMEGTKVRCPCHGSEF
                     NVVTGEVVKGPAKKPEPVYSVREETGELFLDL"
     gene            complement(1286..2515)
                     /locus_tag="VFC43_08560"
     CDS             complement(1286..2515)
                     /locus_tag="VFC43_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31260.1"
                     /translation="MDRKWIMILVVFSLVGIVLLSGCTSQQPATTPTPTVTVQQTPSP
                     KMYSQAETDAKMQWRVLWTDHAVWTRMYIIESLNDSPGADAAAARLLKNQEDIGNAIK
                     PYYGDAAGTKLTGMLKEHILIAVAIINDVKAKNTTAQTADEARWTQNADDIAAFLSSA
                     NPNWRTQDVKDLMHMHLSTTKAELVARYTKDYPADVKAWDTVYTHILTMSDAISSGII
                     KQYPEKFAGPKVFSQKEIDLRNNMRQLWTDHTVWTRLYIIESLNNSPAADAAAARLLK
                     NQDDIGNAIKPVYGDAAGTKLTGLLKDHIMIAVAIINDVKTTNVSAQAADEAKWTSNA
                     DEIATFLAGANPNWPLPVVKDLMHMHLSTTKDELIARYSRNYTADVKAWDTLYTHILG
                     MSDALSAGIVKQFPTGF"
     gene            complement(2660..4852)
                     /locus_tag="VFC43_08565"
     CDS             complement(2660..4852)
                     /locus_tag="VFC43_08565"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HZK31261.1"
                     /translation="MDKKWIIFLLFVSLTGIIVLTGCVSQQPAPNPTATPDLTNQNGP
                     ETATFTAYPALTRLHTPDIPVGFRFVAGGFTAPLDIADPQDGSGRLFVVDQTGYVKIF
                     FMNGTVVEQPFLDVRDRLVKIDPTYDERGLLSLAFHPQFATNGRVFVYYSAPLRAGVD
                     PNWSCTNHLSEFRISASDKNRVDLSSEKILLQVDKPFQNHNGGIILFGPTDGYLYLPL
                     GDGGRADDTGMGHTPGIGNAQDLTKLLGKVIRIDVDHTSPGKLYAIPADNPFLSNSSI
                     APEIYAYGFRNPAYATFDSGGSHKMFIAMAGQRLFESGLIVYKGGNYPWNIREGTHCF
                     NSSNDFLPPAGDCPTTGYSGQPLVGPVIEIGHDVGDTIVGGVLYRGTLMPSLQGTYIY
                     GTWSDENRIVGNGTLLVSKPPADLDLAALPDDASKLTPAQNAMWTTWKMPVVNNANGR
                     INAFIRGLYEDNNHEVLVLINQNGGPGLTPQGSGEIWQMVPATTSGLVSTTARLTGTP
                     TPTATLPKPGMLAAAIQLTIQGDKFNPTNLSVPAGSQVTLTYNDLDLDPHNFALYPSK
                     SISDAVFRSPVITGPVTATYTFNAPATPGTYYFRSDPNTGLLGTFTVTSPTPQTTTVS
                     ATSAPPAQGTTITIILTAKNTAFDTSRISVPAGSKVVMTFINNDANLPHNFALYTDST
                     AGTTIFKGELISGVNTVTYTFTAPSKPGNYFFRCDVHPEMMTGTFVVT"
     gene            5172..>5401
                     /locus_tag="VFC43_08570"
     CDS             5172..>5401
                     /locus_tag="VFC43_08570"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31262.1"
                     /translation="MKKTALSFILVVLIATVFIAGCTQQPAQPTPQPTTVQPADTIKV
                     SDSVLGRILVDAQGKTLYYFANDIPSRANSSC"
BASE COUNT         1238 a         1332 c         1354 g         1477 t
ORIGIN      
        1 ttctgtatct gttatcgtgt tgaactgtgc cccgtgcgtc cggtaggata cgccaaaggg
       61 ggccagcacg taccccatct gctggaaata caggaagata tccatagcgg tctttgttgc
      121 gccgtcttca tgcccggaca ccgtaatacc gacaattttg ccctcggtca ggcccggttt
      181 tttcagcagg tagaggttct ggatacagtt gagtcgttcg agaaaatcct tgaggcgtgc
      241 tgacatgttg ttccagttga tcggcgatcc cacaataacc gcttttgatg cgataatcat
      301 ctcgtggaaa gccggcatgt catcgagctg gtcccggcac gggtacctgc aggcattgtc
      361 ccgcatgctg tagcagcacc agcaatggtt gattgcgaat tcattcaggt tgtagcgacg
      421 ataactgacc cccttttctt tcagaacttc ttcgataaga tccaccatgt accctgtgtt
      481 attggcccgg tgattactgc cgttgatgag gaggacgtca aatttcactc cgtcatacct
      541 gagtttcttt tttaatgaaa tttcctcgaa ttcccggaaa gtgctggcgc actgtgggca
      601 ctcctgggga ggattgtccc ccctggagat gaaaccgcag acatggcatt tccagatatt
      661 atctggcgga acgctggtcg ttttcagttc ctctggtgca gtaaaagaca tgtgaaaatc
      721 cctcggattc aagcaccgga catcccggac agagacaccc gtcttcattt gttatacagg
      781 tactttttcc tcttgccgca agacaaaagg cgatctcttc cccgcagtca ataaacgttg
      841 gacatatttt acagatacag agcgtgattg cttccttcct ctccatactc gttcactctc
      901 ctgatctctc acgtgcttat atattcaggt cacttaaaag tgtgccgaaa aaaaaataaa
      961 aaattttaca aatccaggaa taattcacct gtctcttccc ggactgaata gaccggttcc
     1021 ggtttctttg ccggcccttt taccacttca cctgtcacga cattgaattc cgagccatga
     1081 cagggacacc ggaccttggt gccttccatt ttaccgcctg atagtttaca gcccatatgg
     1141 gtgcaggtat taccgatcgc agtgaatatt ttccctgttt ttacgaccag aatatcgttg
     1201 ccatccgcct cgatctcttt cattggcatg tatgcaaaat ttttttctgt gccgattttt
     1261 tttaccgtca tgcgactttt cagtcctaga agcctgtggg gaattgtttt acaataccag
     1321 ctgataatgc atctgacata ccaaggatat gtgtgtagag agtgtcccat gctttcacgt
     1381 cagccgtata attccgtgaa tatcgtgcaa tcagttcgtc ttttgtcgtg gacaggtgca
     1441 tatgcatcag gtcttttact accggaagag gccagttggg gttggcaccg gcaaggaacg
     1501 tggcaatctc atcagcattg ctcgtccatt tcgcttcatc agcggcctgc gctgagacgt
     1561 ttgtggtctt gacgtcattg ataatggcaa ccgcaatcat gatatgatcc tttaacagcc
     1621 cggtaagttt tgtcccggct gcatcgccat acaccggttt gattgcattc ccgatatcat
     1681 cctggttttt caagagccgt gcagcagcag catcggcagc cggggaattg ttcagtgatt
     1741 caataatgta cagccgcgtc cacaccgtgt gatctgtcca gagctggcgc atgttgttcc
     1801 ggaggtcaat ctctttctgg ctgaagactt ttggaccggc aaatttctcc ggatattgtt
     1861 tgataatccc gctggaaatc gcgtcagaca tggtcaggat atgggtataa accgtgtccc
     1921 atgccttcac atctgccggg taatcctttg tataccgtgc caccagttcg gcctttgtcg
     1981 tggagaggtg catgtgcatg aggtctttta catcctgtgt ccgccagttt ggattggcac
     2041 ttgagaggaa cgctgctatg tcatcggcgt tctgtgtcca ccgggcctca tcagcggtct
     2101 gtgcagtggt atttttggcc ttgacgtcat tgataatcgc gactgcgatc aggatatgct
     2161 cttttaacat cccggtaagt ttcgtcccgg cagcatcccc ataatagggc ttaatggcat
     2221 ttccgatatc ctcctggttt tttaagaggc gggctgcagc agcatcagca cccggggaat
     2281 cgttcaggga ttcgatgata tacatccgtg tccagaccgc atggtctgtc cagaggaccc
     2341 tccactgcat ttttgcatct gtttcggcct gcgagtacat tttgggcgat ggtgtctgct
     2401 gaaccgtaac cgttggtgtg ggggttgttg ccggctgctg gctggtgcat ccactcaaca
     2461 atactattcc caccagggaa aaaaccacta aaatcattat ccattttcta tccatctcaa
     2521 tctccaccct taagggcaat gtaggaaatc catcatacct cttaaaaaga tggtgttttt
     2581 ttccattttt tagaacaaaa aatagaatga atttttcgga ttttgggaaa cggcgaatta
     2641 caaaaaaatt tcttctctct caggttacga caaatgttcc tgtcatcatt tccggatgaa
     2701 catcacaccg gaagaagtaa ttccctggtt ttgacggtgc agtaaacgtg taggtcacgg
     2761 tatttacacc ggaaatcagc tcacccttaa agatcgtggt gcctgccgtg ctgtcggtgt
     2821 acagggcaaa gttgtgggga agatttgcgt cattgttgat gaatgtcatt acaacttttg
     2881 aaccggcagg gacgctgatc cgactggtgt caaatgccgt gtttttcgct gtcaggatta
     2941 ttgttatcgt ggtaccttgt gccggtggtg ctgatgtagc tgatactgtt gttgtctgag
     3001 gtgtgggcga ggttacggtg aatgtcccca gcaacccggt gttcgggtcg ctccggaagt
     3061 aataggtgcc cggggttgca ggagcattga acgtgtacgt tgccgtgaca ggtccggtaa
     3121 ttaccgggct ccggaagacc gcgtcagaga tcgactttga tggatagagt gcgaaattat
     3181 gcgggtcaag gtccagatcg ttataggtga gggtgacctg ggatcctgcc ggaaccgaaa
     3241 ggtttgtcgg gttgaacttg tctccctgta tcgtaagctg gatggctgct gccagcatgc
     3301 cgggctttgg cagggttgca gtcggcgtcg gcgtacctgt cagccgggct gtcgtgctca
     3361 ccagtcctga agtggttgcc ggcaccatct gccagatctc gccagaaccc tgcggggtga
     3421 gaccggggcc gccattctgg ttgatcagga caaggacttc gtggttgtta tcttcataca
     3481 atccgcggat gaaggcgttg atgcgcccgt ttgcattatt tactaccggc attttccatg
     3541 tagtccacat cgcattctgc gctggtgtca gttttgatgc atcatcggga agcgcagcga
     3601 gatcgagatc tgccgggggt tttgaaacaa gaagcgtccc attcccgaca atcctgttct
     3661 catccgacca ggtcccgtat atgtatgtcc cctgcaggga cggcatcagc gtgccccggt
     3721 agagaacacc gccgacaatg gtatctccga cgtcatgacc gatctcaatt acgggtccga
     3781 caagaggttg tccgctgtac ccggtggtcg ggcagtcgcc agccggtggc aggaaatcgt
     3841 tggatgaatt gaagcaatgt gtgccttccc tgatattcca cgggtaattt cctcctttgt
     3901 agacaatgag cccggattca aagagcctct ggcctgccat ggcgatgaac atcttatgac
     3961 tccccccgga gtcaaaagtt gcataggccg ggttcctgaa cccgtacgca tagatctcgg
     4021 gagcaatact tgaatttgac aggaacgggt tatcggcagg aatggcatac agttttcccg
     4081 ggcttgtatg gtctacgtca atgcggatca ccttgccaag gagctttgtg aggtcctgtg
     4141 cgttaccgat accgggtgtg tgtcccatac cggtatcgtc agcccgccct ccgtccccga
     4201 gaggcaggta gaggtacccg tcagtcgggc caaagaggat tatacccccg ttgtggttct
     4261 ggaagggttt atcgacctgt aaaaggatct tttctgatga caggtctacc cggtttttgt
     4321 cgcttgccga gatgcggaat tctgagagat ggtttgtaca gctccagttc ggatcaacgc
     4381 cggcccggag tggtgcacta taatacacaa acacccttcc gtttgttgca aactgcgggt
     4441 ggaacgcaag ggatagcagc cctcgttcat cataggtagg atcgatcttt accaaccggt
     4501 cccggacatc aaggaaaggc tgttcaacga ccgttccatt catgaagaat atcttcacat
     4561 agccggtctg gtcaacgaca aagagtctcc cgctcccatc ttgcgggtct gcaatatcaa
     4621 gaggagctgt aaatccccct gctacgaatc tgaaacctac cggtatatcg ggggtatgca
     4681 gtctggttaa cgcgggatag gcagtaaatg ttgccgtttc aggaccgttc tggtttgtga
     4741 gatccggtgt tgcagtgggg tttggggccg gctgctggct tacacacccg gtcaatacta
     4801 taatcccggt aagggacaca aacagaagga aaattatcca tttcttatcc ataataatct
     4861 ccactcttcg agtattgaca aaaattcctc tcatccttta aaaaattaag tattcttttc
     4921 attttttgga acgaaatctg aaaaaaatct cccagtgccg atgaataaag aaattccttt
     4981 cgtaacatac tgcttctttg cagcccagtt ttacctttga taaatcagtg acataccata
     5041 atgaacggct gggtgctgcg gtacagtcgt gaacccgggg cgtcatagag gaagtttcta
     5101 aaaaatgaaa actatcgaaa gggttatgat ccccgcaggg gattagtctt tgccaaaaaa
     5161 aggtgaattc tatgaaaaaa actgcattat cattcattct ggtcgtttta atcgcgacag
     5221 tcttcatagc cggatgcaca caacagccgg cacaaccgac cccccagccg actacggtac
     5281 aacctgccga tacaataaag gtctctgact cggtactcgg gagaatcctc gttgacgcac
     5341 agggaaagac gctgtactac ttcgcaaatg acattccctc cagggctaac agctcctgtt
     5401 a
//
LOCUS       DASRLV010000225        15776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Methanoregula sp. isolate SMAG_U10198 k141_730867,
            whole genome shotgun sequence.
ACCESSION   DASRLV010000225 DASRLV010000000
VERSION     DASRLV010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963725
            Sequence Read Archive: SRR5665647
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanoregula sp. (soil metagenome)
  ORGANISM  Methanoregula sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanoregulaceae; Methanoregula.
REFERENCE   1  (bases 1 to 15776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:32:26
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,733
            CDSs (total)                      :: 1,694
            Genes (coding)                    :: 1,674
            CDSs (with protein)               :: 1,674
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            complete rRNAs                    :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 6 of 20
            Pseudo Genes (incomplete)         :: 6 of 20
            Pseudo Genes (internal stop)      :: 9 of 20
            Pseudo Genes (multiple problems)  :: 1 of 20
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15776
                     /organism="Methanoregula sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730867"
                     /isolate="SMAG_U10198"
                     /isolation_source="soil"
                     /db_xref="taxon:2052170"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="71.299 N 156.61 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VFC43_08575"
     CDS             <1..199
                     /locus_tag="VFC43_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZK31263.1"
                     /translation="GVIPVLRPLNPVAKLAGTPRPSAERLKKIFAIHERALAEAGLDP
                     RLAVTMCTNCAGCDLVPGKDA"
     gene            196..1467
                     /locus_tag="VFC43_08580"
     CDS             196..1467
                     /locus_tag="VFC43_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZK31264.1"
                     /translation="MRGVEALAEALCRYTDRQYTVPGFPITELGELTKAEMVINEKTA
                     LEYALGDSLSGRRAAVIIKNVGVNACADPLLQAVAQGLVSGLILVAGDDPEALASQTA
                     QDSRYYGELAEVPVIEPDRSSCYSGVETALEASEIFSRIALLRLTPQLLESLVDHNPV
                     PRNDMRGRLSERTWTMNGRVTAAEELYRKMFSWVNTSPLNTWAGEPAGAGAAPGKSRI
                     ITVHPLPDKAAGVLKVQEYGRPFVRDHRGLQPPVPRKRPQSMEERGYYRTFCRGCPFK
                     LMMNIMKERDLSVICDAGCSVLAMTQPYELGLASYGMGASIAVAARSTHVALIGDYAL
                     LHSGLNALIDVYEKELPLLTVIMKNNCTAMTGMQPVYDPIRYLSFAGPVICRTDDELT
                     LKQELVVPDRPRTLVIEGTCPEGSSHETVEY"
     gene            1448..2575
                     /locus_tag="VFC43_08585"
     CDS             1448..2575
                     /locus_tag="VFC43_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase family protein"
                     /protein_id="HZK31265.1"
                     /translation="MKPWNIEICDVTLRDGEQTPGVSFSCEEKVGIARMLDTIGIEVI
                     EAGFPAVSPHEKECVKAVANLGLDARICGFARAREQDVATAIDCNVDMVSIFIPTSEL
                     HVRLKFKKTRGQVLDDALGIIDYATDHGVQVRFAAEDASRTDLPFLKEVYRRASEHGA
                     VLMSFADTVGCLIPSEMQRTMSELVASVDKPLCAHCHNDMGCAVANTITAAEAGAFQL
                     HTTVNGIGERAGNASLEEVLVALRMKGGIDRYDLSLLTELSHMVEKFSGITLPRNKPV
                     TGELAFSHESGIHIAAILEDPSSYEYFPPDMVGSERKFILGKHTGKKALEHVVASIGC
                     ELTEKQVCRVLDLVKDQSEHKCNITPDVLKKLIRKAKEEKA"
     gene            2575..3789
                     /locus_tag="VFC43_08590"
                     /pseudo
     CDS             2575..3789
                     /locus_tag="VFC43_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
     gene            3786..4256
                     /locus_tag="VFC43_08595"
     CDS             3786..4256
                     /locus_tag="VFC43_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HZK31266.1"
                     /translation="MMRSGHAVCMGSDIDTDLVIAGRYLRTKDHAVWVAHVFEDLDPS
                     IANRLKGAVIVAGKNFGCGSSREQAAIAIREAGVVAVVAPSFARIFFRNAINIGLPLI
                     ECDITCSEGSAVMFDLTEGWVENEGKKYPTRPLSPRMQDILSAGGLVNYWRACR"
     gene            4253..5053
                     /locus_tag="VFC43_08600"
     CDS             4253..5053
                     /locus_tag="VFC43_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31267.1"
                     /translation="MIFPERCKEVGYASTKPCGDHVYFLSRYLVRDTGNGHEVLEITT
                     DTASGGMMRRIVASKVIATAMETYRYPEKVQIHDRTRLIEIALDTGFRCTIFTGLDEH
                     MTFVLDPDLSGLLKIHVYDVAPPRPSLSACIRELESAGLFGDLSVSFCHHTRDISLVR
                     ADVYPCRAAGFVKTLDADPMQGGERVAGCITASQFIAECYGKDFVLENICPVENVQDE
                     PFIARCCRSERVGIGTFKGKSGAVVHWGATPSDIARAVNELVIQWKSK"
     gene            5050..6018
                     /locus_tag="VFC43_08605"
     CDS             5050..6018
                     /locus_tag="VFC43_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HZK31268.1"
                     /translation="MTRIAIVEGDGIGHEVIPVAKKVLDLLHPEYEYFSVDVGYHRWE
                     QTGCPCADRDIAELKTAKAILFGAITTPPMKDYQSVVLRLRKALDLYANLRPVKGNGF
                     DIMIVRENTEGLYSGIEEIGTDRATTLRVVTRAGTERIVRTAIRLAKERNGHLTIGHK
                     ANVLKSDVLFRDICSAEAESAGISWNDKFIDALALDVLQHPASYDVIVTTNIFGDILS
                     DMASYLVGGLGLVPSANIGEHYALFEPVHGSAPDIAGKNIANPIAALRSAGLLLSYCG
                     DTQGSNQIESAVQTVIAQGIRTRDLGGSAGTREFGNAVVRALLKRN"
     gene            complement(6157..7029)
                     /locus_tag="VFC43_08610"
     CDS             complement(6157..7029)
                     /locus_tag="VFC43_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZK31269.1"
                     /translation="MAKFCPKCGKPVADESAKFCPNCGLKLPDSSVRENDQNRSGLIP
                     AISPETELPVPLQPIKKRSTLEWVILICVGAVLVVAGLVILSILFLTPVSTITTNASD
                     KNVQSLVTPASATAVPLVVSTPYSDPFPNALPLNSAYHIENTLNNLRYPPELPISTKN
                     DVSIQIKRVALWDSYTVQTPTKYQTVGPSAGKKFVIVIVEVTNYAGNEIIISPYPSNF
                     ELLYDGSHFLPTEIEYPVQAASISYKSESIGRNEQTGGALVYEVPSSLTLNQSHIRLI
                     YLENDEKNPVWHLM"
     gene            complement(7217..7456)
                     /locus_tag="VFC43_08615"
     CDS             complement(7217..7456)
                     /locus_tag="VFC43_08615"
                     /inference="COORDINATES: protein motif:HMM:NF018096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1059 domain-containing protein"
                     /protein_id="HZK31270.1"
                     /translation="MDIQVFCCKDCGLDCSFQVSGMNEREVAKKIIQHMDSDHNMKVI
                     PADFIIKIKHAIKNKSAVKTVHLGPDNKPLIVAMR"
     gene            7863..8444
                     /locus_tag="VFC43_08620"
     CDS             7863..8444
                     /locus_tag="VFC43_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HZK31271.1"
                     /translation="MWWGIGSVILGAAALPLIILIARIVETSLETVRTIYINRGHANL
                     AGWIGIAKTGIWLLSTGIVITNLGDYWNLFAYLAGYGLGTLAGMHIEKMISLGHVIVR
                     LITPADPQSLITELAALGYGMTRIEGSGSFSNTVTIIFMIVPRTELGRLLLILSTQHP
                     DILYTVEDVRNVREGAKIFHKDAKSRILDFFGL"
     gene            8516..8980
                     /gene="msrB"
                     /locus_tag="VFC43_08625"
     CDS             8516..8980
                     /gene="msrB"
                     /locus_tag="VFC43_08625"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HZK31272.1"
                     /translation="MDTPGKQVTQISVFNARTGKTEEVTTVHKTDAEWKAQLTPEQFS
                     VARKQGTEYAFTGKYHASKEHGIYTCVCCGTDLFLSETKFDSGTGWPSFYAPVSRLNT
                     RTHADTFGGMARTEVLCARCGAHLGHVFDDGPPPTGQRYCMNSAALDFIKIP"
     gene            9054..9620
                     /locus_tag="VFC43_08630"
     CDS             9054..9620
                     /locus_tag="VFC43_08630"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HZK31273.1"
                     /translation="MGKISAGRNVFMYPMPVTLLGTLVDKKPDFMALGWVTRVNANPP
                     TIGCGVGRHHHSVRGIEENRTFSINFPSVEMMQKTDYCGLVSGKDVDKAAIFDVYYGE
                     LTTAPMIRECPLSLECQLQSVVENPTNNFYIGEIIASYTEEKYLTDRKLDIRKINPLL
                     LTMPDNRYWTVGACAGDAWSAGKNFKTK"
     gene            9642..10550
                     /gene="uvsE"
                     /locus_tag="VFC43_08635"
     CDS             9642..10550
                     /gene="uvsE"
                     /locus_tag="VFC43_08635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009650 - UV protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HZK31274.1"
                     /translation="MRIGYPCINRSIGCTPSRTFRLASYSDTRLRETIEGNLACLMKT
                     LSYNADHGMLFFRITSDLIPFASHPVCKFPWQIHFADDFLAIGEYIQKHGFRISMHPD
                     QFVLLNTPDQGVLERSIADLTCQAAILDLMELDTSAKLQIHTGGVYGDKPASLERFVS
                     NYQQLDDSVKKRLVVENDERLYTISECMALHERTGIPVLFDVFHHTLNNNGEKTSDLF
                     APLSATWKKHDGIPMVDYSSQQPKKRAGAHAESIDPEDFVVFLNSTIPFDMDIMLEIK
                     DKEKSALLALGLAHDDTRLVLGSGKA"
     gene            10587..11147
                     /locus_tag="VFC43_08640"
     CDS             10587..11147
                     /locus_tag="VFC43_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HZK31275.1"
                     /translation="MDLLTPSLVGIGLSMDCFAVSLAFGSTIKARLSYAAITVALCFG
                     AFQAGMTLIGWGAGISIIKLISSYDHWVAFILLAIVGGKMVWEGISGEDQEKPIKSIR
                     FYPLIVLSIATSIDALAAGVSFGVLQATILLPAVIIGIICFVISFAGVMLGERLETVL
                     GNKMEILGGIILILIGVKILCDHMIG"
     gene            complement(11265..11720)
                     /locus_tag="VFC43_08645"
     CDS             complement(11265..11720)
                     /locus_tag="VFC43_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HZK31276.1"
                     /translation="MKTEDAKKIISTAIDREVEAYTFYRTISDKVKDPALKKLFNELA
                     GEEKQHREFLQGMLSKDILKMHFDAKADYKVVNAMPSPPQSADMKPLDGIIVSIKKEL
                     EAMQMYTQLANLSTDTEQKLLFSQLANMESGHKARLEDLYTNMAFPEVW"
     gene            complement(11870..12916)
                     /locus_tag="VFC43_08650"
     CDS             complement(11870..12916)
                     /locus_tag="VFC43_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HZK31277.1"
                     /translation="MNNLPTPLSFCIILYKDGRIDRLVDRPLEEYLIAIRDASIAWVD
                     CSTKDDNKEIEQIALIAGFSKIPIPKLTTGFYSAYEDYDTELGIMLPSATVQAEKMAV
                     HPLFILIRDNLVLTVHCEEINRLLRFSRYAPPFFKKVAEESLVDKVTMILERIIDENN
                     DRNFEYLREIEMHGDAISKSLIEENLAKRKIAHDIYHMKHVLIDYLNVLWSTKDVVDS
                     LRYGDADLITNDEKLLGRIGILSDNIDRHIELSEQMSNVLSSGLEVMQSIYNNQLQNL
                     NNRFALVTAYLTVLGTAFLVPNTIATIAGSGVMEGTMGAQWWYVPLLILSTVVATLAS
                     FLWVLHVWKMKEEE"
     gene            13096..13698
                     /locus_tag="VFC43_08655"
     CDS             13096..13698
                     /locus_tag="VFC43_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HZK31278.1"
                     /translation="MSWLTRFSAVTLCMFIVSGGCTSITPISPQSPASLTTTGSPPPA
                     LTPSSALTIRVASLSMGSVLPDIYTCKGSGISPAVSWDGVPPGTKSLVLILDDPDAPN
                     GSFTHWIVFNIPPETGELPQGQPNAKVLAIGAQQGDTSTGSRGYYPPCPPIGATHHYV
                     FRLYAVDMDITQPTADRSSIEWSLNGHTLANAEFVTIFKR"
     gene            complement(13710..14921)
                     /locus_tag="VFC43_08660"
     CDS             complement(13710..14921)
                     /locus_tag="VFC43_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK31279.1"
                     /translation="MLPAGGINRFLSPDRQMMYYLFLLGFFAIFSTTLSKNPVLPLFS
                     QAIGANDTVIGLVAAVSPLAGILFSFPVGVLSDYIGRKRLLITSGVVFFSAPLLYLII
                     TDPLWLIPVRFFHGTATAILGPVISAVIAERFPEKKGAMLGQYSSATLIGRTVAPLAG
                     GVIISSFLMYPGLIPYRMVYATAALAAVPVLIMTLMYRENHQVPQGRIPVSRFRSSFV
                     TFFSHAGLRATAFVDMATYFSFGAFETFLPLFLASQGFDPYLTGIIFAVQVLIIAGTK
                     PFFGKIADHFDKRIQIVAGLIILGSSVALIPFTTGFIPILFVSAVFGLGISLSTVATS
                     AYVADIAQKEQIGASMGALSSTMDIGHSAGPLVTGIVITSIGYSSGFFMSFLLAVIIA
                     VFFAISVRDKK"
     gene            15021..15773
                     /locus_tag="VFC43_08665"
     CDS             15021..15773
                     /locus_tag="VFC43_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK31280.1"
                     /translation="MKKCVSDGIVVLVLMAALMFLPVSGIVYPPPTTSSTGLNTTIPD
                     NSDVIATMAAKHFVSATVSSGSPAVGEPVTIGGRVTGGVLSEGVQIWVFAGNYVNVSH
                     VPVKANGTFSHTFPTTGLPLSTYYVYIQSPGANGMFNIDLEDKGIYAGQVVNTGTNAL
                     IFNFTGTGSVQDAAASRALSDAINNQGFDDAYTKLTFQLVPPGTTPIAMETSGIPEPA
                     AATTKSPLPLSITCLAVVIAASSAVLYVRKRG"
BASE COUNT         4064 a         3712 c         4047 g         3953 t
ORIGIN      
        1 cggagtgatt cctgttcttc gccccctcaa tccggtggcc aaacttgctg gaacaccccg
       61 tccgtctgcg gaacggttga agaaaatctt tgcgatccat gaacgggctc ttgcggaagc
      121 cggactggat cctcgtctgg ctgtaacaat gtgtactaac tgtgcgggct gtgatcttgt
      181 gcccgggaag gatgcatgag gggcgtcgaa gctctggcag aagccctctg cagatataca
      241 gaccggcagt ataccgttcc ggggttccct atcaccgaac ttggtgaatt aacaaaagcg
      301 gaaatggtga tcaacgaaaa aactgctctc gaatatgcac tcggtgattc actgtccgga
      361 aggcgggctg cagtcatcat caaaaatgtc ggggttaacg cgtgtgcgga tcctctcctc
      421 caggctgttg cccaggggct ggtttccggg ctcattctcg ttgccggaga tgatcctgaa
      481 gccctggctt ctcagaccgc acaggactcg cggtactatg gcgaacttgc agaagtgcct
      541 gtcattgaac ccgacagatc cagctgttac agcggggtag aaacagcgct ggaggcctcg
      601 gaaatattct cacgtattgc gctgctccgc cttacccccc aacttctgga atctctggtt
      661 gatcacaacc cggttccccg gaatgacatg agggggaggt tgtctgaacg gacctggacc
      721 atgaacgggc gggtgacggc tgcagaggaa ttataccgga aaatgttttc ctgggttaac
      781 acatccccat tgaatacctg ggcaggagaa ccagccggag ccggagccgc accggggaaa
      841 agccggatca ttactgttca tcccctgccg gataaggcag caggtgtcct caaagtacag
      901 gagtacggca ggccttttgt ccgcgatcac cgtggactcc agccaccggt cccccggaaa
      961 agacctcagt ctatggaaga acggggatat taccgcacat tctgccgggg atgtccgttc
     1021 aaattgatga tgaatatcat gaaggagagg gacctgagcg tgatctgcga tgcgggctgt
     1081 tcggtccttg ccatgacaca gccctatgaa ctgggtctgg caagttatgg gatgggtgca
     1141 agcattgctg ttgccgcacg aagtacccat gtcgcgctca taggggatta tgccctgctc
     1201 cattcggggc tgaatgcact gattgatgtc tatgaaaagg agcttccgct cctgactgtt
     1261 ataatgaaga acaactgcac ggctatgacc ggaatgcagc cggtgtatga tccgatccgg
     1321 tacctttcct ttgcaggtcc ggttatctgc cgtacagatg atgaactgac gctgaaacaa
     1381 gagctcgtgg tcccggacag gccgcggaca ctggttattg aagggacatg tccggaagga
     1441 agcagccatg aaaccgtgga atattgagat ttgtgatgta acgctcagag acggggagca
     1501 gacaccaggt gtttcattct cctgtgagga aaaggttggg attgcccgaa tgctggacac
     1561 catcggcatt gaggtgatag aggccgggtt ccctgcagta tccccccatg agaaggaatg
     1621 cgtaaaggca gttgcgaacc ttggtctcga tgcccgcatc tgcgggttcg cacgagcccg
     1681 ggaacaggat gttgcaacag cgattgactg caatgttgac atggtaagca tctttatccc
     1741 gacatccgag ctgcacgtcc ggctcaaatt caaaaaaaca cgggggcagg tgcttgacga
     1801 tgcgctcggg atcatcgatt atgcgacgga tcacggtgtg caggtgcggt ttgcagctga
     1861 ggatgcgtcc cggacggatc tacccttcct aaaagaggtg taccgtcgtg cgtcagagca
     1921 cggtgcggtg ctgatgagct ttgccgatac cgttggctgt cttatcccat ccgagatgca
     1981 gcggaccatg agcgaactgg ttgcatcggt ggacaagcca ctctgtgctc actgccataa
     2041 cgacatgggc tgtgcggtcg caaacacgat tactgcagca gaagccgggg ccttccagct
     2101 tcacaccacg gtgaatggca ttggtgaacg ggccggcaat gcatcgttgg aagaagtgct
     2161 tgtggccctg cggatgaaag ggggtattga tcggtacgat ctctcgctcc tgacagagct
     2221 ttcccatatg gtcgaaaaat tctccggtat caccttaccg agaaacaagc ctgtcaccgg
     2281 cgaacttgca ttctcgcacg agagcggcat ccacattgct gcaattcttg aagatccttc
     2341 ctcgtacgaa tacttcccac ccgatatggt aggaagcgaa cgcaaattca ttctcggaaa
     2401 acatacgggg aaaaaagcgc ttgaacatgt ggttgcatcg atcggttgcg aacttaccga
     2461 gaagcaggtc tgccgggtcc tcgacctcgt caaggatcaa tcagagcata aatgcaacat
     2521 cacccctgat gttttaaaaa aactgatcag aaaagccaaa gaggagaaag cctgatgggg
     2581 acgttatcag agaagatcct tcacgggacc gggggacaat atgttgaccg gggagttgac
     2641 cgggcctatg tccatgacgg caccggtgtg cttactctcg aagcctggaa gtgtatgggg
     2701 gagaggagaa tacaggacgc tacacatctc catgtcctct tcgatcatat cgtcccggca
     2761 aacaacggta cgactgcgac gctccagcac gagctgcggg agtttgcccg aagttcgttc
     2821 attaactttt cggatgtcgg gggcggtatc tgtcaccaga tcatgagtga ggggatagtt
     2881 cttcccggtg aagttgtggt cggcgctgat tcgcacagct gcacgcttgg tgcttttggt
     2941 gcattcgcca ctggggttgg cgcaacggat atggcggcga tctggatgac aggggagacg
     3001 tggttccggg tgccggaatc ggtagggata aaccttaccg gaacgctttc gggtgcagca
     3061 gaggcaaagg atcttgctct cacctacgta ggccggctcg ggacggacgg tgcaacctac
     3121 caggcactcg aatttatcgg agatggcgcg gctgcactct cgatggatga ccgattaaca
     3181 ctctgcaacc ttgctgtcga aaccggagca aaggccggga tgttctatgc ggatgatgta
     3241 acggtgcagt atttggcaga gcgcgggcac acagtcaatg gacagtaacc ggaaccctgc
     3301 cggtatattc aggagctcga tttcgatctt tcggatattg tcccggttat tgctgttcct
     3361 caccgtgtgg acacaataaa accggtacat agtcttgccg gacttcccct cgatcaggtt
     3421 tttgtgggta catgtacaaa cgggcgcttt accgatctcg cccgtttcgc acaaatagtg
     3481 aaaggaaaga aggtcgcaat ccgcacaatc gtcgttcccg cttcacggga agtattaaaa
     3541 gaagcgaccc ggaccggggt gtttgcatcc cttgtcgaag ctggatgtac catcggaact
     3601 cctggttgtg gcccctgtct tggagcacat atgggtgtac tgggtgaagg cgaggtctgc
     3661 ctgtccacgg cgaaccggaa ttttaaaaac cgtatgggtg tcggagggga gatttacctg
     3721 tcatcggttg ctaccgctgc agccagtgca cttgaaggag aaataaccga gccggaggtg
     3781 gtcagatgat gaggagtggg cacgcagtct gtatgggttc ggatattgat actgacctgg
     3841 tgattgccgg gcgctatctt cggacgaagg accatgcagt ctgggttgcg cacgtgtttg
     3901 aagatcttga tccttcgatt gcaaatcgac tcaaaggtgc tgtgatagtg gcaggtaaaa
     3961 attttggctg cgggtcatcg cgggagcagg cggcaattgc catacgggaa gccggcgtgg
     4021 ttgcagttgt tgccccctcg tttgccagaa tttttttccg caatgcgatc aatattggtc
     4081 tcccgcttat cgaatgtgat attacctgta gtgaaggaag tgcggtgatg ttcgacctca
     4141 ccgaaggatg ggtggagaat gagggaaaaa aatatccaac ccgcccgctt tccccgagga
     4201 tgcaggatat cctttcagca ggtggtcttg ttaattactg gagggcctgc agatgatatt
     4261 tcctgaacgg tgcaaggagg tagggtatgc atccacaaaa ccctgtggag atcatgtcta
     4321 tttcctgagc cgttacctgg tgcgtgatac cggcaatggg cacgaagtgc ttgaaatcac
     4381 aaccgatacc gccagcggcg gaatgatgcg gaggatcgtg gcatcaaagg taattgcgac
     4441 agccatggaa acatatcgct accccgagaa agtccagatt catgaccgga cgcgactgat
     4501 cgaaattgca cttgatacgg gattccgctg cacaatattt accggccttg atgagcacat
     4561 gacatttgtg ctggatcccg atttatccgg actgcttaaa atccatgtct atgatgtggc
     4621 acccccccgc ccgagtcttt ctgcctgtat ccgcgagctg gaatcagccg ggctttttgg
     4681 agatttatca gtctcgtttt gtcatcatac cagggatatc tcacttgtca gggctgatgt
     4741 ctatccctgt cgtgcagccg gttttgtaaa aacactcgac gccgatccga tgcagggagg
     4801 tgaacgcgta gccggatgca taaccgcctc acagtttata gcagaatgtt atggcaagga
     4861 ttttgtgctt gagaatatct gccctgtgga aaacgttcag gacgagccgt ttatcgcccg
     4921 ctgttgtcgt tccgagcgtg tggggatcgg gacgttcaag ggcaagtctg gtgccgtggt
     4981 gcactggggt gccaccccct ccgatatagc ccgggcggtg aatgaactgg tcatacaatg
     5041 gaagtcgaaa tgacccggat tgcaattgtt gagggagacg gaatcggtca tgaagtgatc
     5101 cctgtagcaa aaaaagtcct ggatttgttg cacccggagt acgagtattt ctctgttgac
     5161 gtaggatacc accgctggga acagaccgga tgtccctgtg cagatcggga tattgcagaa
     5221 ctgaagactg caaaagcgat cctgttcggg gcgatcacaa cccctcccat gaaggattac
     5281 cagagcgtag tactgcgact gagaaaagca ctcgatctgt atgcgaacct gcggccggta
     5341 aaaggcaacg ggttcgatat catgatcgtg cgcgagaata cagaaggact ctattccggc
     5401 atcgaagaga taggtaccga ccgggcaaca acgctccggg tagtgaccag ggccgggacc
     5461 gagaggatcg tccgaactgc aataaggctg gcaaaagagc ggaatggtca tctaacgata
     5521 gggcataagg caaatgtgct caaatccgat gtactgttcc gcgatatctg tagtgccgaa
     5581 gcagaatctg ccggtatatc ctggaatgac aagttcattg atgcactggc gctcgacgtc
     5641 ctccagcatc cggcatctta cgatgtcatt gtaaccacaa acatctttgg agatattttg
     5701 tcagatatgg cctcctatct tgtcggaggt ctgggtcttg tgccaagtgc aaatattggt
     5761 gaacactatg cgttatttga gccggtgcac gggagcgccc cggacatcgc tgggaaaaat
     5821 atcgctaacc cgatcgcagc tttgaggagt gccggattac tcctgagcta ttgtggtgat
     5881 acccagggat cgaaccaaat tgaatctgca gtacagaccg tcattgcaca gggtatacga
     5941 acccgggatc tcggaggatc tgcgggaacc cgggagttcg ggaatgcagt agtccgggct
     6001 ttattaaaaa ggaattaatg aatgtgatat tctccaaaaa ggcaacaacc ctccccaaga
     6061 ctttccgtat ctcaatagtc atcttcaatg tcgcggggtt gtggatagca tggctaaatc
     6121 agatcatgac ttggaaaaaa caggaaatca aaaatattac atcaaatgcc aaacgggatt
     6181 tttttcgtca ttctcaaggt aaatcaaccg aatatgcgat tgattaagcg tcagagatga
     6241 tggtacttcg tataccagtg cccctcctgt ttgttcgttt ctgccaatac tttcggattt
     6301 atagctgata cttgcggctt gaaccggata ttcaatttct gtcggtaaaa aatgtgaacc
     6361 atcatagagt aattcaaaat ttgatggata gggtgaaata attatttcat tacctgcata
     6421 attcgttact tcaacgataa caatcacaaa tttttttccc gctgacggac ccacggtttg
     6481 atattttgtt ggtgtctgga cggtatatga atcccataat gcaacccttt ttatctgtat
     6541 ggaaacgtca ttttttgttg aaattggcag ttccggtgga tatcgaagat tattgagggt
     6601 attctcaatg tgatacgcag aattcagggg cagtgcgttg gggaaaggat cactgtacgg
     6661 agtggaaaca accaatggaa cggcagtagc ggatgcagga gtcacaaggg attgaacgtt
     6721 cttatccgat gcattggttg ttatggtaga tactggtgtc aaaaatagga ttgaaagaat
     6781 tacgagtcct gcaaccacaa gaactgctcc aacgcatata agaataaccc attcaagagt
     6841 cgatcgcttt tttattggtt gaagaggaac cggcagttcc gtttctggtg atattgccgg
     6901 tattaaacca gatcggtttt ggtcattttc tctcactgat gaatcaggga gcttcaaccc
     6961 acagtttgga caaaattttg cactttcatc tgcaactggt tttccacatt tcggacaaaa
     7021 ttttgccatg tctctctgtt ctccccttaa atcccgataa atagcaattc tatggattta
     7081 acaataagta tatcggtttt tcatggagcg tccggatccg gtttttatct tccggcagga
     7141 taggattcca gattttcccc gtggatatat ggcacgcaga aaagataaga aaaatgaaag
     7201 aaaggggcgt gagtggttat ctcatcgcca caatgagagg cttattatca ggaccgagat
     7261 gaacagtttt gactgcactc ttgtttttaa tcgcgtgttt gattttgatt atgaaatctg
     7321 ccgggatgac tttcatgtta tgatcagagt ccatgtgctg gatgatcttt tttgcaacct
     7381 cacgttcgtt cattccggaa acctggaatg aacagtccag cccgcaatcc ttacaacaaa
     7441 agacctgtat atcaatcgcc tcttacgaat tgcaacatta tccgcttaga ctattaaata
     7501 aatgcaaggc gatgacgcaa tgcagaccgg cggcatacag gttgaactat ctctcatttc
     7561 agcgacagcg ggtgtcaggg acagtcacag gcctgggaat tacaaaaata cctggcattt
     7621 tctcgcatgc agtaatgatg cgggccgggc cagtcaataa aaaaccaata ttttgaagaa
     7681 tgcaatcccc tgggtggcca ttataacctc ccctttaaaa aatacgacaa aagacaccaa
     7741 cggcgcagtc tgcgtgtttt catgctactt atcctgatag tccgtattct ctgaatcccg
     7801 cttaattaca atctcattcc gcaaggtatt caagatgtac cgaaagacaa gtatgatcag
     7861 gagtgtggtg gggaatcgga tcagtaattc tcggggcagc agcattaccc ctcattatcc
     7921 tcatcgctcg tatcgttgag accagcctgg aaacagtcag gacaatctat atcaatcgcg
     7981 gacatgcaaa ccttgccggc tggataggaa ttgctaaaac cggtatctgg ctgctttcga
     8041 caggtattgt gatcaccaat ctcggggatt actggaacct gtttgcctat cttgcaggct
     8101 acggcctggg gacccttgct ggaatgcata ttgagaaaat gatatccctc ggtcacgtga
     8161 tcgtgagact tatcactccc gctgatcccc agtctttaat aaccgagctc gctgcactcg
     8221 ggtatggtat gaccaggatc gaaggctcag gaagtttttc caatacagtc acaatcatct
     8281 ttatgatcgt tccccgtaca gaacttgggc gtttgctgct aatcctttca acacaacatc
     8341 cggatattct ctatactgtg gaagatgtgc gcaacgtcag ggaaggagca aaaatttttc
     8401 ataaggatgc caaaagcagg atactggatt tttttggttt ataacattcc caaagtccta
     8461 gaccgatctg ttctgatcaa aagctctatg ggtctgccat cccatcactc agatcatgga
     8521 tacaccagga aaacaggtaa cccagatctc cgttttcaat gccaggaccg ggaagaccga
     8581 ggaagtcact acggtacata agaccgacgc tgaatggaaa gctcaactca ccccggaaca
     8641 gttcagtgtg gcacggaaac agggcaccga gtatgctttc actggtaaat accatgcttc
     8701 aaaagaacac ggaatctata cctgtgtctg ctgtggcacg gatctctttt tatcggagac
     8761 caaatttgat tcaggaaccg gctggcccag tttttatgcc ccggtttcac ggcttaatac
     8821 ccgtactcat gccgatacct ttggggggat ggcacgcaca gaagtgcttt gtgcacgttg
     8881 cggggcgcat ctcgggcacg ttttcgatga cggtccaccc ccaaccggcc aacgctattg
     8941 catgaattct gcggcgctgg atttcattaa aatcccgtaa gagtgctatg gaatgactgg
     9001 taacaatcct tttttaaggt gccctgccca tagcagagct ggtgaatgaa tctatgggaa
     9061 aaatatcagc gggaaggaat gtgtttatgt atcccatgcc ggtaaccctc ctcggtacac
     9121 tggtggataa aaaaccagat ttcatggcgc ttgggtgggt aacccgcgtt aatgccaatc
     9181 ctccaacaat tggctgcggt gtggggcgtc accaccactc ggttcgcggg atcgaagaaa
     9241 accggacctt cagcattaac ttcccgtcag tggaaatgat gcagaaaacg gattattgcg
     9301 gcctcgtctc aggaaaggat gttgacaagg cagcaatctt tgacgtgtat tatggcgagc
     9361 tgacaacagc tcctatgata cgtgaatgtc cgctttcgct ggaatgccag ttacagtccg
     9421 tggttgaaaa tccgaccaat aatttttata ttggtgaaat catcgcctcc tacactgaag
     9481 agaagtatct caccgatcgt aaactggata taaggaaaat caacccgctc ctcctgacta
     9541 tgccggacaa ccgttactgg accgtcgggg catgtgctgg cgatgcatgg agtgcaggaa
     9601 agaactttaa aacgaaatga ggcaacgttt agaccacgta tatgcgtatc ggatacccct
     9661 gtattaaccg ttcgataggt tgcactccct cacgtacgtt tcggcttgca tcatactcag
     9721 atacaaggct gagggaaacc atcgaaggaa acctggcctg tctcatgaaa actctctcgt
     9781 acaatgcaga ccacggcatg ctctttttcc ggataacctc cgatttgata ccctttgcat
     9841 ctcatcctgt ctgcaaattc ccctggcaaa ttcacttcgc agacgatttt ttagctatag
     9901 gagaatatat tcagaaacac gggtttcgga tatcaatgca cccggatcag tttgtcctcc
     9961 tgaatacccc tgatcagggt gtccttgaac ggagtattgc cgatttaacc tgccaggcag
    10021 cgattctcga cctgatggag ctcgatactt ccgcaaaact ccagatccat accggagggg
    10081 tgtatgggga taaaccagcc agtctggaga ggtttgtctc aaattaccaa cagctcgatg
    10141 attcggtgaa aaagcgtctt gttgtagaaa atgatgaacg actctatact atttcggaat
    10201 gcatggccct gcatgaacgt acgggaatac cggtgctttt tgatgtgttt caccacaccc
    10261 tcaataacaa cggtgaaaag acaagtgatc tgttcgcacc ccttagtgcg acatggaaaa
    10321 aacatgatgg catccctatg gtggattaca gttcacagca accgaaaaag agggcgggtg
    10381 cacatgctga gagtatcgat ccagaggatt tcgtggtttt cctgaattcg actataccct
    10441 ttgatatgga tatcatgctg gaaataaaag ataaagaaaa aagtgctctc ctggcactcg
    10501 gtcttgcaca tgatgataca aggctggttc ttgggtctgg taaagcatga ttttattata
    10561 gttccttgag tacaagtccg ggaatcatgg atctcctcac cccgtcgctt gttggaatag
    10621 gtctttcaat ggattgtttt gccgtttcgc ttgccttcgg ttcgacaata aaggcgcggc
    10681 tttcatacgc agcgatcacc gtagcgcttt gctttggcgc attccaggcc ggcatgaccc
    10741 tgatcggatg gggagccggt atttcgataa tcaagctgat ctcttcgtat gatcactggg
    10801 ttgcatttat cctgctggcg attgtcggcg ggaaaatggt atgggagggt atatctggtg
    10861 aggatcagga aaaacctatc aaaagtatcc ggttttatcc tttgatcgtg ctttcgattg
    10921 cgacgagtat cgatgcgctt gctgcgggcg taagtttcgg agtgctccag gccacaattc
    10981 tcttacctgc tgttattatt gggattatct gtttcgtgat ttcctttgcc ggcgtgatgc
    11041 ttggggagcg tctggaaact gtgctgggta ataaaatgga gatcctcggg ggcattatcc
    11101 tgatcctgat tggtgtaaaa atcctttgtg atcacatgat tggctaaaaa acgcaggtat
    11161 tttaattatg aaatggcggc agaaccaaac cacggttccc tgaactcctc ctaaaaaaag
    11221 tggatgcata atgcatcctt tgaattttga atctgtccaa ccgtttacca gacttcgggg
    11281 aaggccatgt tggtgtacag gtcctcgagc cgggctttgt ggccgctttc catgtttgca
    11341 agctgggaaa agagcaactt ctgctctgta tcagttgaca ggttcgcaag ctgcgtgtac
    11401 atctgcatag cttcaagttc ctttttgatt gaaacaatga tgccgtcgag gggtttcatg
    11461 tcagcagact gtggggggct cggcattgca tttaccactt tgtaatcagc ctttgcgtca
    11521 aagtgcatct tcaagatatc ctttgagagc ataccctgca aaaattccct gtgttgtttc
    11581 tcttccccgg cgagttcatt aaagagtttt ttcagtgccg ggtcttttac cttgtcagat
    11641 attgtgcggt aaaaggtgta tgcctcgact tctctgtcga tggcggttga aatgatcttc
    11701 tttgcatctt ctgtcttcat ttcactactc ctccattaag taaattatta ccaattaccc
    11761 tatataagaa cattggtttt acctcctgac gaagagaaag aatctctttt catccgaaaa
    11821 gatcggtgag gaatataaaa aaagttctct atcctgagaa ttctgtctgt cactcttcct
    11881 ctttcatctt ccagacatga agcacccaga ggaaagaagc cagagtagca actacggtgg
    11941 agagtatgag taatggtaca taccaccact gagcccccat cgtcccttcc atgaccccgc
    12001 ttccggcaat ggtcgctatg gtatttggaa ctaaaaaagc ggttcccaga acagtcagat
    12061 aggcggtgac gagggcaaac cggttattca ggttctggag ctggttatta taaatgcttt
    12121 gcatcacttc gaggccagat gaaagcacgt tggacatctg ttctgaaagt tcgatatgcc
    12181 gatcgatatt gtcagataat attccaatcc gcccaagaag tttttcatcg ttcgtgataa
    12241 ggtcagcatc cccgtaacgc aacgaatcga ccacatcttt cgtcgaccag agcacgttca
    12301 ggtaatcgat cagcacatgt ttcatgtgat agatatcatg ggcaatcttt ctcttggcaa
    12361 ggttctcttc gatgagtgat ttgctgatcg catcgccatg catctctatc tcgcggaggt
    12421 actcaaaatt acggtcatta ttctcatcga taatccgttc cagtatcata gtgactttgt
    12481 ccaccagtga ctcttctgcg acttttttaa agaatggcgg agcgtaccgg gaaaatctca
    12541 ggagccggtt aatttcttca caatggactg tgaggactaa attgtcgcgg ataaggataa
    12601 aaagcgggtg aaccgccatc ttctctgcct gaacggttgc agaagggagc attatgccca
    12661 gctcagtgtc ataatcttca taagccgaat aaaaacctgt agtcagtttt gggatcggga
    12721 tcttggaaaa tccggcaatc agggcgatct gctcgatctc tttgttatca tccttagtac
    12781 tacagtcaac ccatgctata ctcgcatcgc ggatggcaat aagatattct tcgagaggac
    12841 ggtctaccag ccggtcgatc ctgccatcct tatagagaat tatgcagaaa cttaaaggtg
    12901 taggtaagtt gttcatggat ttttgcgggg tactttgcat tttcataaca gcacctcata
    12961 aggtcagacg gtattattgt gccacgtggt atacgagcta ttgataccgc gagtgtatat
    13021 aggtggctgt ttttatgata tccctgcccc cgctagtcgg aatccttaat acaatggatg
    13081 agcctgtttc aaggtatgtc atggttaaca cgtttctcag cggtaactct ctgtatgttt
    13141 attgtgtcag gcggatgtac ttcaattaca cccatttccc cacaatctcc tgcctcgcta
    13201 acgaccacag gttccccccc tcctgccctg acaccttcca gcgcactgac gatcagggtc
    13261 gcctctctct ccatgggttc ggtccttcct gatatctaca cgtgcaaggg ttcaggtata
    13321 tctcccgcag tatcatggga tggtgtcccg cccggaacaa aaagtcttgt cctgattctt
    13381 gatgaccccg atgcaccaaa cggcagtttt actcactgga ttgtctttaa catcccgcca
    13441 gaaaccggag aactccccca gggacaaccg aatgcaaaag tactggccat cggtgctcag
    13501 cagggtgata ccagcacagg ctcaagagga tattatccgc cctgcccccc aatcggggca
    13561 acacaccatt atgtgttcag gttatacgca gttgatatgg atattacaca gccgaccgca
    13621 gatcgcagct ctattgaatg gtcactgaac ggacatactc ttgcaaatgc ggagtttgta
    13681 acgatattta agcggtgatt gacaaggttt tattttttat cccgtaccga aattgcaaaa
    13741 aaaacagcga tgataacagc cagaaggaaa ctcataaaga atccggatga gtagccgatg
    13801 ctggtaataa caattccggt cacaagcggt ccggccgaat gcccgatatc catggtagag
    13861 gagagtgccc ccatcgatgc cccgatctgt tctttttgtg caatatccgc tacatatgca
    13921 cttgtcgcga cggtagacag cgatatgcca agaccaaaca cagcactgac gaaaagaatg
    13981 ggaataaaac cggtagtgaa cgggattaaa gctacagaac tgccgaggat gataaggccc
    14041 gcgacgatct gaatgcgttt atcgaaatgg tcagcaattt tcccgaagaa aggcttcgtc
    14101 ccggcgatga tcagcacttg tacagcaaaa ataatccccg tcaggtaagg atcgaatccc
    14161 tgcgacgcga gaaagagggg aagaaacgtt tcaaaagcac caaacgaaaa gtacgtagcc
    14221 atatcgacaa aagctgtagc ccggagcccg gcatgggaaa agaaggttac aaaacttgag
    14281 cgaaagcggg agaccgggat tcgaccctgt ggtacctgat gattctcgcg gtacatgagg
    14341 gtcatgataa gaaccgggac agctgcaaga gcagcagtcg catataccat gcgataagga
    14401 atcagtccag ggtacatcag aaaagaggag ataatgacgc ccccggcaag gggggcaact
    14461 gtccgcccaa tgagtgttgc cgaggaatac tggcccagca ttgcaccttt tttttcgggg
    14521 aaccgttctg caataaccgc agaaatgaca ggaccaagga tggcagtggc ggttccatga
    14581 aaaaatctta ccgggataag ccagagggga tcagtgatga tcagatataa aagcggcgca
    14641 gaaaaaaaca ccacacccga cgtgatgagt agtctctttc gccctatata gtctgataga
    14701 acgccgacgg gaaacgagaa cagtatcccg gctagtggag atacagcagc cacaagccca
    14761 atcactgtat cattagctcc tatcgcctgg gagaaaagcg gtaataccgg gttttttgac
    14821 agagtggtgg aaaatatcgc aaagaaaccg agcaggaaca gataatacat catctgacga
    14881 tcaggagaga gaaatcggtt gataccgcct gcgggtaaca tgggtgttaa cgggataggc
    14941 agtgatcggg tttaagagta ctgataatag cgaacttgaa aaccaatata ctaatagtga
    15001 ttacagtcaa aaaatcccct atgaagaaat gtgtatcaga tggaatcgta gtgctggtgt
    15061 taatggcagc acttatgttt ctcccagtct ccggaatagt gtatccacct cccacgacat
    15121 cttcgacagg attaaacacc acaataccgg ataattcgga cgtgattgcc acaatggctg
    15181 caaaacattt tgtaagtgca acagtctcct cgggttcgcc agcagtgggt gagccggtta
    15241 caatcggagg gagagtaacc ggaggcgtcc tatcggaggg tgtccagatc tgggtgtttg
    15301 ccggcaacta cgtgaatgtt tcacatgtac cggtcaaggc aaatgggaca ttctcccata
    15361 catttccaac aaccggattg cccctttcaa cgtattatgt gtatatccag agccccggtg
    15421 caaatgggat gttcaatatc gatttagagg ataaaggcat ctatgcgggt caggttgtca
    15481 acaccggtac caatgcgttg atcttcaatt ttaccggaac aggaagtgtt caggatgcgg
    15541 cagcttcgcg ggctctctca gatgcaatca acaaccaggg ctttgatgat gcgtatacaa
    15601 aactcacttt ccagctggta ccccccggga caactccaat cgccatggaa acctctggta
    15661 ttcccgagcc ggcagccgca acgaccaaat ccccactccc cctttcgatt acctgtctgg
    15721 cagtggtcat agccgcttca agtgcggtgt tgtatgtaag aaaacgagga taacgt
//
